LOCUS       DACEUZ010000001        84475 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.001, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000001 DACEUZ010000000
VERSION     DACEUZ010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 84475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 84475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..84475
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.001"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(21..818)
                     /locus_tag="IXG44_000001"
     CDS             complement(21..818)
                     /locus_tag="IXG44_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HAQ1656749.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(815..1999)
                     /locus_tag="IXG44_000002"
     CDS             complement(815..1999)
                     /locus_tag="IXG44_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAQ1656750.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(2024..3271)
                     /locus_tag="IXG44_000003"
     CDS             complement(2024..3271)
                     /locus_tag="IXG44_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virion core protein"
                     /protein_id="HAQ1656751.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            3486..5231
                     /gene="dnaX"
                     /locus_tag="IXG44_000004"
     CDS             3486..5231
                     /gene="dnaX"
                     /locus_tag="IXG44_000004"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HAQ1656752.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            5251..5565
                     /locus_tag="IXG44_000005"
     CDS             5251..5565
                     /locus_tag="IXG44_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HAQ1656753.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            5590..6186
                     /gene="recR"
                     /locus_tag="IXG44_000006"
     CDS             5590..6186
                     /gene="recR"
                     /locus_tag="IXG44_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HAQ1656754.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            6250..6894
                     /locus_tag="IXG44_000007"
     CDS             6250..6894
                     /locus_tag="IXG44_000007"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HAQ1656755.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            6909..7238
                     /locus_tag="IXG44_000008"
     CDS             6909..7238
                     /locus_tag="IXG44_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1656756.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQL"
     gene            7252..8190
                     /gene="holB"
                     /locus_tag="IXG44_000009"
     CDS             7252..8190
                     /gene="holB"
                     /locus_tag="IXG44_000009"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HAQ1656757.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            8226..9050
                     /locus_tag="IXG44_000010"
     CDS             8226..9050
                     /locus_tag="IXG44_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HAQ1656758.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            9043..9390
                     /locus_tag="IXG44_000011"
     CDS             9043..9390
                     /locus_tag="IXG44_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HAQ1656759.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            9459..10331
                     /gene="rsmI"
                     /locus_tag="IXG44_000012"
     CDS             9459..10331
                     /gene="rsmI"
                     /locus_tag="IXG44_000012"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HAQ1656760.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            10440..11561
                     /gene="dinB"
                     /locus_tag="IXG44_000013"
     CDS             10440..11561
                     /gene="dinB"
                     /locus_tag="IXG44_000013"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HAQ1656761.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(11615..12217)
                     /gene="nrdG"
                     /locus_tag="IXG44_000014"
     CDS             complement(11615..12217)
                     /gene="nrdG"
                     /locus_tag="IXG44_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HAQ1656762.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(12532..14721)
                     /gene="nrdD"
                     /locus_tag="IXG44_000015"
     CDS             complement(12532..14721)
                     /gene="nrdD"
                     /locus_tag="IXG44_000015"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HAQ1656763.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            15047..15814
                     /locus_tag="IXG44_000016"
     CDS             15047..15814
                     /locus_tag="IXG44_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1656764.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            15804..17846
                     /locus_tag="IXG44_000017"
     CDS             15804..17846
                     /locus_tag="IXG44_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1656765.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            18127..18276
                     /locus_tag="IXG44_000018"
     CDS             18127..18276
                     /locus_tag="IXG44_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HAQ1656766.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            18287..19801
                     /gene="dltA"
                     /locus_tag="IXG44_000019"
     CDS             18287..19801
                     /gene="dltA"
                     /locus_tag="IXG44_000019"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HAQ1656767.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            19798..21003
                     /gene="dltB"
                     /locus_tag="IXG44_000020"
     CDS             19798..21003
                     /gene="dltB"
                     /locus_tag="IXG44_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HAQ1656768.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            21048..21281
                     /gene="dltC"
                     /locus_tag="IXG44_000021"
     CDS             21048..21281
                     /gene="dltC"
                     /locus_tag="IXG44_000021"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HAQ1656769.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            21284..22552
                     /gene="dltD"
                     /locus_tag="IXG44_000022"
     CDS             21284..22552
                     /gene="dltD"
                     /locus_tag="IXG44_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HAQ1656770.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            22693..23775
                     /locus_tag="IXG44_000023"
     CDS             22693..23775
                     /locus_tag="IXG44_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HAQ1656771.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            23775..24578
                     /locus_tag="IXG44_000024"
     CDS             23775..24578
                     /locus_tag="IXG44_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAQ1656772.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            24648..25388
                     /locus_tag="IXG44_000025"
     CDS             24648..25388
                     /locus_tag="IXG44_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAQ1656773.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            25399..26403
                     /locus_tag="IXG44_000026"
     CDS             25399..26403
                     /locus_tag="IXG44_000026"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HAQ1656774.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(26448..28292)
                     /locus_tag="IXG44_000027"
     CDS             complement(26448..28292)
                     /locus_tag="IXG44_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1656775.1"
                     /translation="MSFFNNIYNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSP
                     ADSNQMKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLL
                     IKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNY
                     AGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSL
                     ILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKK
                     WYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNR
                     FYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNT
                     DKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKN
                     YVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDD
                     LAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGIT
                     EELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
     gene            28504..29022
                     /locus_tag="IXG44_000028"
     CDS             28504..29022
                     /locus_tag="IXG44_000028"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAQ1656776.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            29150..29833
                     /locus_tag="IXG44_000029"
     CDS             29150..29833
                     /locus_tag="IXG44_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HAQ1656777.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            30142..30215
                     /locus_tag="IXG44_000030"
     tRNA            30142..30215
                     /locus_tag="IXG44_000030"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:30176..30178,aa:Arg,seq:tct)
     gene            30374..30733
                     /locus_tag="IXG44_000031"
     CDS             30374..30733
                     /locus_tag="IXG44_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HAQ1656778.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            30856..32487
                     /locus_tag="IXG44_000032"
     CDS             30856..32487
                     /locus_tag="IXG44_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HAQ1656779.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(32549..33190)
                     /locus_tag="IXG44_000033"
     CDS             complement(32549..33190)
                     /locus_tag="IXG44_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAQ1656780.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     gene            33472..33780
                     /gene="rplU"
                     /locus_tag="IXG44_000034"
     CDS             33472..33780
                     /gene="rplU"
                     /locus_tag="IXG44_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HAQ1656781.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            33807..34151
                     /locus_tag="IXG44_000035"
     CDS             33807..34151
                     /locus_tag="IXG44_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HAQ1656782.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            34164..34457
                     /gene="rpmA"
                     /locus_tag="IXG44_000036"
     CDS             34164..34457
                     /gene="rpmA"
                     /locus_tag="IXG44_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HAQ1656783.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            34864..37698
                     /locus_tag="IXG44_000037"
     CDS             34864..37698
                     /locus_tag="IXG44_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1656784.1"
                     /translation="MAKRIDRIYMYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNS
                     SKDLNQLVREGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDT
                     KDIFAKIIGANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAH
                     HVIDEETELIVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLD
                     EIHRLPPEGQEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRI
                     PINIKLPAFEKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQL
                     KSNIQLVCARGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKIT
                     VAPNDTIMKIQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFY
                     KDHGFSFNADSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKK
                     INLGEERHTNDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLR
                     ANQASGRIGVVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNV
                     QEVDEGSGVILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDL
                     DMLHESLKNFRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNK
                     RQETELEVLALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVI
                     LDQLLMESELEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTT
                     GIGDEEYGFKINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLED
                     QIKVKVPIEEMYYLLRLVHNQLDKKEYTVP"
     gene            37889..38380
                     /locus_tag="IXG44_000038"
     CDS             37889..38380
                     /locus_tag="IXG44_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIB"
                     /protein_id="HAQ1656785.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            38483..39472
                     /gene="manX"
                     /locus_tag="IXG44_000039"
     CDS             38483..39472
                     /gene="manX"
                     /locus_tag="IXG44_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HAQ1656786.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            39505..40308
                     /locus_tag="IXG44_000040"
     CDS             39505..40308
                     /locus_tag="IXG44_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HAQ1656787.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            40329..41243
                     /locus_tag="IXG44_000041"
     CDS             40329..41243
                     /locus_tag="IXG44_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HAQ1656788.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            41326..41700
                     /locus_tag="IXG44_000042"
     CDS             41326..41700
                     /locus_tag="IXG44_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HAQ1656789.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            41839..42084
                     /locus_tag="IXG44_000043"
     CDS             41839..42084
                     /locus_tag="IXG44_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ1656790.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            42256..43320
                     /locus_tag="IXG44_000044"
     CDS             42256..43320
                     /locus_tag="IXG44_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAQ1656791.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            43566..43991
                     /locus_tag="IXG44_000045"
     CDS             43566..43991
                     /locus_tag="IXG44_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ1656792.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            43984..44433
                     /gene="nusB"
                     /locus_tag="IXG44_000046"
     CDS             43984..44433
                     /gene="nusB"
                     /locus_tag="IXG44_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HAQ1656793.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            44530..45384
                     /locus_tag="IXG44_000047"
     CDS             44530..45384
                     /locus_tag="IXG44_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HAQ1656794.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            45385..46722
                     /locus_tag="IXG44_000048"
     CDS             45385..46722
                     /locus_tag="IXG44_000048"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HAQ1656795.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            46727..46951
                     /locus_tag="IXG44_000049"
     CDS             46727..46951
                     /locus_tag="IXG44_000049"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HAQ1656796.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            46953..47843
                     /locus_tag="IXG44_000050"
     CDS             46953..47843
                     /locus_tag="IXG44_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAQ1656797.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            47852..48670
                     /locus_tag="IXG44_000051"
     CDS             47852..48670
                     /locus_tag="IXG44_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HAQ1656798.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            48777..49226
                     /locus_tag="IXG44_000052"
     CDS             48777..49226
                     /locus_tag="IXG44_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAQ1656799.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            49244..50926
                     /gene="recN"
                     /locus_tag="IXG44_000053"
     CDS             49244..50926
                     /gene="recN"
                     /locus_tag="IXG44_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HAQ1656800.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(50981..51097)
                     /locus_tag="IXG44_000054"
     CDS             complement(50981..51097)
                     /locus_tag="IXG44_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HAQ1656801.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(51170..52126)
                     /locus_tag="IXG44_000055"
     CDS             complement(51170..52126)
                     /locus_tag="IXG44_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAQ1656802.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            52297..52665
                     /locus_tag="IXG44_000056"
     CDS             52297..52665
                     /locus_tag="IXG44_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HAQ1656803.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            52835..53266
                     /gene="mraZ"
                     /locus_tag="IXG44_000057"
     CDS             52835..53266
                     /gene="mraZ"
                     /locus_tag="IXG44_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HAQ1656804.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            53287..54246
                     /gene="rsmH"
                     /locus_tag="IXG44_000058"
     CDS             53287..54246
                     /gene="rsmH"
                     /locus_tag="IXG44_000058"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HAQ1656805.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIGLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            54251..54652
                     /gene="ftsL"
                     /locus_tag="IXG44_000059"
     CDS             54251..54652
                     /gene="ftsL"
                     /locus_tag="IXG44_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HAQ1656806.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            54653..56845
                     /locus_tag="IXG44_000060"
     CDS             54653..56845
                     /locus_tag="IXG44_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAQ1656807.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKTGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            56871..57833
                     /locus_tag="IXG44_000061"
     CDS             56871..57833
                     /locus_tag="IXG44_000061"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HAQ1656808.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            57847..59208
                     /locus_tag="IXG44_000062"
     CDS             57847..59208
                     /locus_tag="IXG44_000062"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HAQ1656809.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            59234..60322
                     /gene="murG"
                     /locus_tag="IXG44_000063"
     CDS             59234..60322
                     /gene="murG"
                     /locus_tag="IXG44_000063"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HAQ1656810.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            60337..61557
                     /locus_tag="IXG44_000064"
     CDS             60337..61557
                     /locus_tag="IXG44_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HAQ1656811.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            61713..63038
                     /gene="ftsA"
                     /locus_tag="IXG44_000065"
     CDS             61713..63038
                     /gene="ftsA"
                     /locus_tag="IXG44_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HAQ1656812.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            63061..64302
                     /gene="ftsZ"
                     /locus_tag="IXG44_000066"
     CDS             63061..64302
                     /gene="ftsZ"
                     /locus_tag="IXG44_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HAQ1656813.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            64319..64996
                     /locus_tag="IXG44_000067"
     CDS             64319..64996
                     /locus_tag="IXG44_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HAQ1656814.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            65010..65609
                     /locus_tag="IXG44_000068"
     CDS             65010..65609
                     /locus_tag="IXG44_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HAQ1656815.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            65624..65911
                     /locus_tag="IXG44_000069"
     CDS             65624..65911
                     /locus_tag="IXG44_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HAQ1656816.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            65960..66742
                     /locus_tag="IXG44_000070"
     CDS             65960..66742
                     /locus_tag="IXG44_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HAQ1656817.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            66763..67470
                     /locus_tag="IXG44_000071"
     CDS             66763..67470
                     /locus_tag="IXG44_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HAQ1656818.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            67732..70530
                     /gene="ileS"
                     /locus_tag="IXG44_000072"
     CDS             67732..70530
                     /gene="ileS"
                     /locus_tag="IXG44_000072"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HAQ1656819.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(70579..72105)
                     /locus_tag="IXG44_000073"
     CDS             complement(70579..72105)
                     /locus_tag="IXG44_000073"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HAQ1656820.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(72120..72767)
                     /locus_tag="IXG44_000074"
     CDS             complement(72120..72767)
                     /locus_tag="IXG44_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HAQ1656821.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(72951..73280)
                     /locus_tag="IXG44_000075"
     CDS             complement(72951..73280)
                     /locus_tag="IXG44_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HAQ1656822.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            73457..74185
                     /gene="sfsA"
                     /locus_tag="IXG44_000076"
     CDS             73457..74185
                     /gene="sfsA"
                     /locus_tag="IXG44_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HAQ1656823.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            74201..75214
                     /locus_tag="IXG44_000077"
     CDS             74201..75214
                     /locus_tag="IXG44_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ1656824.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            75214..76491
                     /locus_tag="IXG44_000078"
     CDS             75214..76491
                     /locus_tag="IXG44_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAQ1656825.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            76554..79256
                     /locus_tag="IXG44_000079"
     CDS             76554..79256
                     /locus_tag="IXG44_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1656826.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            79408..79725
                     /locus_tag="IXG44_000080"
     CDS             79408..79725
                     /locus_tag="IXG44_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1656827.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            79755..80075
                     /locus_tag="IXG44_000081"
     CDS             79755..80075
                     /locus_tag="IXG44_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAQ1656828.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            80183..81643
                     /locus_tag="IXG44_000082"
     CDS             80183..81643
                     /locus_tag="IXG44_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1656829.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            81711..81932
                     /locus_tag="IXG44_000083"
     CDS             81711..81932
                     /locus_tag="IXG44_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1656830.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            81963..82145
                     /locus_tag="IXG44_000084"
     CDS             81963..82145
                     /locus_tag="IXG44_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HAQ1656831.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            82145..82558
                     /locus_tag="IXG44_000085"
     CDS             82145..82558
                     /locus_tag="IXG44_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HAQ1656832.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            82681..83862
                     /locus_tag="IXG44_000086"
     CDS             82681..83862
                     /locus_tag="IXG44_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1656833.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            84251..84324
                     /locus_tag="IXG44_000087"
     tRNA            84251..84324
                     /locus_tag="IXG44_000087"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:84285..84287,aa:Arg,seq:cct)
     gene            complement(84393..>84475)
                     /locus_tag="IXG44_000088"
     CDS             complement(84393..>84475)
                     /locus_tag="IXG44_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HAQ1656834.1"
                     /translation="HIVRELEDQDKEKINTVFDSLYKNDN"
BASE COUNT        27511 a        14481 c        17917 g        24566 t
ORIGIN      
        1 gaacattcat ttagcaattt ttagaaactt ctgccgccac cgccaaaagt cccgccaccg
       61 ctagaatggg tagtacttcc gccaccgccg cttccaggag gtgggctttt ggggattctt
      121 cttgtcgtga caaaagaatt tgtcagacga tcggtttttt cagttaattt gatactggat
      181 ttttctcgga atggatattt gtagatgcct gatttcaatt gataacgcga aacagtcaca
      241 aaataaaaga caagactaag gataagagcc aaaacaacag caatgatgat ttctactgtc
      301 gtcaaaacct tatagcgtgt aattttgcca gtttcttcgt ctacacgata atgtcctcct
      361 ggaactccgt cttccacata tgttgctgat ttagacaggt aagcttttgc agcattgtaa
      421 taggcagcat ttgtcatctg atcataaaca tcatctaaga tcgaatcgat tctgctatca
      481 gtcaaataat cgatcatatt accagaagtc gaaatgtaga tttcacgctg tcccatatct
      541 aatagcaata ctgagccatt ttgatcattt ccgactgcgt ctcttaagta attatcagca
      601 aagtctcttg gttcttctga attagatgta gtcgttacga tcaagactcg tcctttgatt
      661 ttttcattca gcttttctgc ctgttgttcc aaagattgga tttgatctgt tgtgaataac
      721 ccagcctcat catttaccgt agctgtatct gcatgagtag ccaccggcca gccaacactt
      781 actagcaaaa gaaagaataa aaaaaatttt ttcatcatat caagtaacct ccgatcagaa
      841 agaggatcgc tagcccagcg aaaatcccaa gagcggtcag tcccaatttt ttctggctga
      901 tcggcaggac accgctgacc tttcctgttt gtccattcat tgcgtagtag tagacttttt
      961 tacttgagtc attgcttcga taagtaacaa gccataccgg cagtaatacg taattattta
     1021 cttcgtttgt gattgaagca gaagttcgca caccggtcaa tgttgtatat ccatttgcgg
     1081 tatcttttaa cagcttctct gaatagtctt ttaattcaga ttgaatctgt gatttgattg
     1141 cctcatattc gatatctctt ttttctgctt ggaacccggc taaatactgg cttttaaaat
     1201 caattgcttt atctaacggg aaaggttgca cgccttcgac catcttttgt tgcgtatttt
     1261 ttgataaggc attttttact agttctttaa aagaaagctt gcctgctctt gaaacagcaa
     1321 attgcttcgt ttctgtgtat tcgatgtcac ctatccgcca tatgcggata actgtaccat
     1381 tcgcctgcat ggcaccatct acttcagcat ctgtagccca ataagggaaa tacacacctg
     1441 tcaacttatc gatttgatcc ttattgaaaa aagctttggg aacaaaccat tttttcttgg
     1501 tccaagcgag aaatctgttg attgcttctt ctttctcgac agcaaaagga agtactttat
     1561 ttggcaagaa ttctccgctt aaacgtcccg atagtacgac gggattatgg cagtagtaac
     1621 aataagtcgc agcagttgtc gcatcagtca cgatctctgc accacagttg ggacaaagaa
     1681 aaagatctat cgctgcggta ttatctgcag attcgtccgc tgtatctgaa aaaccgcctg
     1741 cctcgtcaac cgctttgcgc tcggcttcat tcatatcggc aagctgttct tgggctgtaa
     1801 aagtaaaatc ttcggtaggt gaaggttctt ctttgctttc ttcgccagcc atacgcgctt
     1861 ctttttgctt ttgctcatat tggctgactt cttcttctgt atagatattc aggcagtatt
     1921 cgcaatgaaa tttttggtct ttgggatcaa aagtcaacgg accgccgcaa ttaggacatt
     1981 tatgtgtaag aacatccatc agctgcacac ctttctaaat aaattaatta acggggattc
     2041 ctttaaacgg tttaccacag tttggacaga atttagggat gccgttggaa agatccacga
     2101 tctcattgca ttcactgcat cgcatttgta gcttaggctg aacgttttct acaggtttgg
     2161 cagctccgca gttggaacag aattttccgg aattttctgt cccgcaaact ggacatgtcc
     2221 aagtatccgc atttccttgt gcagtttgtt gattttgttt ttctgcttgt tgtttgattt
     2281 gttcttggtt attttgagct gcttgagaaa gataagaacc atttgcattc attcccatac
     2341 ctacacccat aaagccggtc attgctcctg cttcattttt tccggctgct tccataccac
     2401 gtgcaatgga accttgcacg tatccttcac gtacgctagg atcacctagc atcgcccctt
     2461 cgttacgcat attgatcagt ttcacagaat catcggtgta agaaatgctg gctacagcca
     2521 cagaaacaat ctccatccca cgtaattcac gccaagaatc atccaatgca gtatccatgt
     2581 atttgcttaa ttccaaactc ttagatggta cataagaaat ccgttgacca tctgcagaca
     2641 tctgattgat tgctgattgt agcgctgtta ggaattcagc taaatactgc tcattgatat
     2701 cattgatttc cacttgtgtt ttgtttttag ggattgcgtt tgtataaaat aaaatcgggt
     2761 caacgacatg aatagaataa gtgccatgtg cccgtaagaa taattctgcg ttatagaaat
     2821 tgtcaaagta gttcaacggc gcgcttgtac cgaatttgat tccctttatt tcttggagat
     2881 tgatataaaa aacttgttgt ttctgcggtg tgacgccgcc aaatttgaat cggtcaaaag
     2941 tttcagcaat tgcttctttc agtgttccat tgaacatgga aggagctgcg tcatttttaa
     3001 tggtgtaata accttcctca gcggtataat cgatgatttt tccaccatcg accaaaagca
     3061 tcatcatgtt tggatagaca tggacgactg ttccgtctgt taaaacatct tctgttcctt
     3121 tacgattgga gccacgtttg tcatctttgc ggactttgac accttttgtc atgaccgttg
     3181 tatcactcat attgtctggt tcgatgactt ctagccattg atctgccaga ccgccgccaa
     3241 ccatacttgt tgctgctttg ataagaccca taaaaaattc ctcctctgat tcagctagtg
     3301 ccaaacaatt attgtttatt tattatacca taggaagatg tgaaacttct cggactagag
     3361 acggaattga aatggaaaat tgcgtattgt ttaaatacct gaaaaagccg agaaggatag
     3421 tagttttttt acctaatatc acgtataata agagatagtg aattttttaa aaaaggggga
     3481 tccttatggc atatcaagca ctttatcgag tctggcgttc gcagcgtttc gaagacatcg
     3541 ttggacaaaa agctgtgaca cagacactaa aaaatgctat tgtttcccac aaaacctcac
     3601 atgcctactt gtttacagga cccagaggaa ccggaaagac gagtgcagca aagattttcg
     3661 caaaagcaat caactgtccg aacagtaaag acggtgagcc atgcaacgaa tgcgaaatgt
     3721 gtcgatcaat tactgctggt acgcaagaag atgtgatcga aatcgatgct gccagcaata
     3781 acggagtcga agaaatccga tttatccgtg atcgggcaaa ttatgcgcct acaaaggcag
     3841 cgtacaaagt gtacattatt gatgaggtac atatgctttc aacaggagct ttcaatgcct
     3901 tgttgaaaac attggaagag ccaaaagaaa gtgtcatatt catcttagca acgaccgaac
     3961 ctcacaaaat accagctacg atcatttcac ggactcagcg atttgatttt aaacggatca
     4021 atacggcaga tattgtcgaa catcttgaat ttatcttgaa agaatcgact atcagctatg
     4081 aaccagctgc attgaatgtg attgcccgtt ctgcagaagg cgggatgcgg gatgcattga
     4141 gtattcttga tcaggcaatc tcgtttagtg aaggaacaat tactttagat gatgccatgc
     4201 aagtgacagg tagcttaact gacgaaatga tggacagtta cttgagtgct tgcgtgaaaa
     4261 aagaagtacc agaagcattg gaaacattga accaaattct cgcagccggc aaagaatcac
     4321 gtcgctttct agaggatatg ttacaatact gccgtgactt gctgatgtat cagcaagcac
     4381 ctaacctagt tgaggaacaa tcgagtacga tcagaagtac atttacagaa ttggcacaaa
     4441 ctgtttcagc ggatcagctg ttttcaatga taaagatttt gagcgatact caaaatgaaa
     4501 ttcgttttac caatagtgcc gctatttatt tggaagtcgc tacagtcaaa cttgcacagc
     4561 taccgtctgc ttctgttcct tcagcagatg tagcatttgc agatgatcag atagtaaaac
     4621 agctacaaca agagattaag gaactaagaa acgaaatcaa ggaactacgc agtaatacag
     4681 gaattgagaa tacaaaagta cagccggtac agcaaacaaa tcataaaaaa cagacaagta
     4741 cctatcgtgt accgacggaa cgagtatata aagttctgcg ggaagcgaca agacagcatc
     4801 tgaaccaagt aaaagaagta tgggaagact tgctgatgag tctatctgtc actcagcgag
     4861 caatgcttaa agcaagcgaa ccagttgcag ctggacctac aggactagtg attgcgtttg
     4921 attatgagat tgtttgtcaa agagcagcaa atgacgaaga attgcagctt tcggtacaca
     4981 acaacttaag ccgaatgatc aaagattatg cgcctgatcc tgtttatatt acaagagaaa
     5041 gctggccaga acttcgaaga aattatttga cgcaggcaca agaagaggga acagaaaact
     5101 cttttgcttc tgaagactca gaaggagaaa tcgagttgct tcctcaagaa caagaagtac
     5161 cagaagaaac acttgtagta aaagcagagg aactgtttgg ctccgaagct gttacagtgg
     5221 tagatgatta ataaaaagga gtagataatt atgatgcgtg gaatgggcaa tatgcaaggc
     5281 atgatgaaac aagtacaaaa aatgcaaaaa gaaatggcag aagcacaaga gctgctaaat
     5341 aaaacagagt ttacaggtaa agccaccaat gaattggtca aagcggtttt tacaggcgat
     5401 cgtcgcatga aagatatcca aatcaatgaa gaagtcgttg atcctgatga tatcgagatg
     5461 cttcaggatc ttgtcgtaat ggcagtcaat gatgcgatcg cacaaattga tgtggaaact
     5521 gagaaaaaat tagggaaata cgcaaaaggt ttgccaggat tttagtttta aactgaagga
     5581 gtaatgaata tgcaatatcc agaaccaatt gcgaaactga tcgacagcta catgaaactt
     5641 ccaggaatcg gtcagaaaac agcgacaaga ctcgcctttt atacgatcga tatgaaagat
     5701 gaagtagtaa atgaatttgc taaatctttg ttaagtgtaa aacgcgattt gcatttttgt
     5761 agtatctgcg gaaacatcac agaagaagat ccctgcgaaa tatgtagaaa tccttcacgt
     5821 gatcgaagcg ttatcctagt ggtcgaagag ccgaaagatg taatggcaat ggaaaaaatg
     5881 cgagaatatc acggacttta tcatgttctc catggtgtgt tatcaccgat ggaagggaca
     5941 ggtccagaag acatcaatat ttcttctctg ttgcagcggc tgcatgatgc agatgtaaaa
     6001 gaagtgatta tcgcaacaaa tgcaacaaca gagggagaag caacagctat gtatctctca
     6061 cgtctgatca aacccgcagg tatcaaagtg actcgtctag cacatggtct gtctgtaggc
     6121 tctgatatcg agtatgcaga tgaagttact ttattgaaag ctgtcgaagg aagacgtgag
     6181 ttataaattt ctaattaagt gttttttcag atacaatata aaagcagcac aagagatggg
     6241 ggcaacaatg tgaacggact atttattaca atcgaggggc ctgatggtgc aggaaaaacc
     6301 agcatcttga atgaacttta ccctcttttg aaagaagtag caaaaacaga aatcattcaa
     6361 acaagagaac caggaggcat tccgattgct gaagagatcc gagcagtgat ccttgatcca
     6421 aaaaatgatc ggatggatga acgtacggaa gcactgcttt atgcagcagc aagaagacaa
     6481 catctagtcg aaaaggttct gcctgctctg gcaaaaggga aaatcgtttt atgcgataga
     6541 tttgtcgaca gttctcttgc ttatcaaggt gcaggccgac gcatcggcgt taccgagatt
     6601 gctcggttga atgagtttgc tacagaaggt actacgccgg attttaccct ttatcttgac
     6661 gtagattcag acaccggatt aagaagaatc aagaaaaaca gacaaaatca aatcgaccgt
     6721 ttagattcgg aaggtttgga attccaccag cgtgtacgtc atgcttactt aaagctggca
     6781 gaagagaatc cggaaagaat ccacaaagtt gatgcaagaa aaagctttga agaagtgctg
     6841 caaacaagtt atcatacaat tattgaacag tatccgcaat tttttgaaaa ctaggaaggt
     6901 gaacgtttat gaaaattatt ttagcgatta tccaagacaa agacagcaac cgtttatcaa
     6961 atgaattgat tgatgcaaat atccgtgcca caaaactatc atcgacaggc ggcttcctta
     7021 aggctggaaa cagtacgttt atcgtgggta ttgaagatga ccgtgtagaa gaagcattgg
     7081 aaatcatcaa aaaaacgtgt gaatcaagaa agcaatttgt atctacacct gttacactgg
     7141 atatttcgat ggatgggggc gtcccttatc cagtagaagt agaagtaggc ggtgccacag
     7201 tcttcgtctt acctgttgaa ggattccatc aactttagga ggagctgagc tatgaatcaa
     7261 gaaataaact tggctcaaat gcaaccgatc gtgtatcagc agcttcaacg gagttttgag
     7321 catgggcgta tcgctcatgc ttatcttttt gaaggggaga aaggaacagg aaaacatgaa
     7381 gtaggtatat ggcttgcaca gcacctgttc tgtacccaaa tgaaagacaa tctgccttgt
     7441 ggaaaatgca acaattgtca gcggatcgaa aagaaagaac atccagatgt tttggttatt
     7501 gaaccggaag gacaaaccat caaagtcgat cagatcagac gtttgcagac ggaattcagt
     7561 cgcagcggtt acgaatcaag aaaaaaagtt tttttgatca aagaagctga aaaaatgaat
     7621 agcagtgcgg caaacagttt gttgaaattc ttggaagagc cccctggaga ttttttagcg
     7681 gtgttagaaa cagatgcagt aggaagaatt ctccctacta ttcaatctcg ttgtcaagtt
     7741 ctgcattttc aagaactttc gaaagaagca ctgatccatc gattgcaaga agagcagatt
     7801 ccattggaaa aagcaaaact tttggctttc ttaacaaaca gtttaggaaa agcagttgaa
     7861 atctctcaag atgaatggtt taatgatgct aaggacttga tttatcaatg gttcgtttac
     7921 ttgcaaaagc aagatactca agcctttatc tatgtccaaa aaaaattggt aaaatcattt
     7981 aaagaaaaaa cacaacagtt tacaggatta tctgtcttga tgttttatta tcaggaagca
     8041 cagcgtcaag cagtagcaaa cggcgaatat ggcaaagtga aacagataaa ccagataatt
     8101 gaacggatat tgatcgcaga gcagaaacta cgcagcaatg tcagttttca ggctgttgcg
     8161 gaacagttcg ttttgcatac gattttttag cttgccgcaa aggatgatga tcaaggagga
     8221 tggaaatggt agaagtagta ggtgtccgat atagagatgc aggtcatatc tattattttg
     8281 caccaggaga ctctgaatat gtttatagtg acaaggtatt agtcgaatct cagcaatcct
     8341 tacatattgc tactgttgtc cttcctaaaa aagagattga tcccaatgac cttcctgaag
     8401 ctatccgtcc gatcttgcat aaagcgtcac aagctgaatt agaaaaagac aaaaaaaaca
     8461 aaaaagatgc ttatgatgca ttgaaaatcg caaaagaaaa agtgcagttg cgtgagttgg
     8521 aaatgaaaat ggtcaaagcc gaatacacat ttgatcgaaa taaattgatt ttttctttca
     8581 cagcggacgg gcgaattgac tttagagaat tggtaaaaga tctggccgct gtatttcgta
     8641 ctcgtattga gcttcgacaa attggtgtcc gcgatgaagc aaagataata ggcggaattg
     8701 gtccatgtgg gcgtccatta tgctgttctt cttttttagg cgatttcgtg cctgtatcaa
     8761 tcaaaatggc aaaagatcaa agtttatctt tgaatccaac aaaaatttcc ggtttatgcg
     8821 gacgcttgat gtgctgtctg caatatgaga gcgaagaata cgagacagcg aaaaaagaac
     8881 tccctgactt tggaaaagta attgctacac ctgatggtaa agggaaagtc gtagggttga
     8941 atctgcttag ccgtatcgtt agtattcggc tgactggacg ggaaaatcct gtcgaatatg
     9001 aatgggatga attgaaagaa tttatccaag taggtgagat gaatggataa gagatcatta
     9061 tacgacggat tgaatcaatt agaatcagag ttgagaaata cattaacaga acttgttgaa
     9121 atgaagcatg ctttgcatga gatcgtagaa aaaaatacaa ctttggagat ggaaaatcaa
     9181 cgtctgcggg aacacctgcg ggaattgaac caagttgcac aagctccagc tgaaaatgtg
     9241 aagcaggaac tttctaagtc tcgtatgaat cttgaaaaaa tttatgaaga aggattccat
     9301 gtctgctacg atttatatgg atcaagaagg gacaatgacg agccttgtgc gttttgtcta
     9361 gaagtgatct atcgcgaaag cggtcgatga ccaataacaa gaggagactg gagcggagaa
     9421 atactgctcc agtcttttta ttaaggagat tgtgacagat gtataatcaa aaaagtttca
     9481 agggaaatcc ggtatatggg gatttatatc ttgtaccgac gccaattgga aatttagaag
     9541 atatgagcta cagaagtgtt cgtatgctgc aagaatccga tcttatcgca agtgaggata
     9601 cgcgtaatac acaaaagtta ttgaatcatt ttgaaatcca tacaccccaa aaaagtttac
     9661 atgaacataa ttataaagaa cgtgtccctc aattgattgc tttgttaaaa gaaggaaaaa
     9721 cgattgctca agtcagtgac gcaggaatgc cttccatcag cgatcctgga catgagctag
     9781 ttttagcctg cataaaagaa gggatttctg tgatctctat cccaggacct acagctggga
     9841 tgacggcttt gattgcttca gggctagtgc ctcaaccatt tttattttat ggcttccttc
     9901 cacgtaagaa aaaagaacag aaagaaacat tgaaaaagct aaaagaacag acagttacgc
     9961 tgatttttta tgagtcacct tatcggatca ctgctacatt gacgaatttc ttagaaattt
    10021 ttggaaatcg ccaagtcgtt ttgtgtcggg aattgacaaa ggtccatgaa gaatacctta
    10081 gaggaagtac ggaagagcta ctcgcttata tcgaagaaca tccagtaaag ggtgaatgtt
    10141 gtctgcttgt agaagggaat ttgttcgaaa tgccagtgac ggaagttgat gaagagttgg
    10201 gaacgttaaa ggaacaagta gaagcaaaga tcgcttcagg cgaaaaacca aatgctgcta
    10261 tcaaagcagt agcgctgaaa aatgggctga aaaaacaaga agtctaccgt cagtaccatg
    10321 aattagactg agatagtata agttgtctat aatgaaagac taatgaggaa atcagctact
    10381 aacttttcaa atctcttgaa cgcgaacgaa tgttcttgta taatgaataa gaggtgagga
    10441 tgatggagct gcaatttcct ttacataatg atacatcaag aaaaatcatt catattgata
    10501 tggatgcttt tttcgcttcg gttgaagaac gagatcatcc tgagtttcgc ggacatccat
    10561 tagtaattgc cagacacccc agtgacactg gtgggcgtgg agtagtgact acagcaaatt
    10621 atgaagcaag aaaattcgga attcattcag caatgagtgc acaaaaagcg tatgagcttt
    10681 gtccgcaagc catctttaag cctggcgacc atcataaata cagcgatatt tccaaacaag
    10741 tacgggaagt attctatcgt tatacagata ttgtggaacc agttagtatc gatgaagcgt
    10801 atcttgatgt gaccgaaaat aaaatcaatt gcaagtcagc catcaaaatc gcccgaatga
    10861 tccaagcaga tatttgggag agtgtccagc taacatgttc tgcgggagta agttacaata
    10921 agtttttggc caaattagca tcagactatc aaaaacctag aggaatcacg gtagttgccc
    10981 ctcaagacgc tgtgccattt ttaaaagctt tgccaattga aaaatttcat ggggtaggga
    11041 agaaaactgt acctagaatg catgagctag ggatttatac aggaaaagac ctatatgaat
    11101 gcacagaaat gatgttgatt cgaaattttg gaaaaatggg ctattctctt tatcgtaaag
    11161 tcagaggaat ccatgattct ccagtaaacg ttacaagaga gcgaaaatct gtgggaaaag
    11221 agcatactta tgggcagccg ttgcaaacgg aagaagcagt gctgactcag ttgcgccaac
    11281 tagctgaaaa agtagaagaa gcactgagaa gagtccaaaa acatgggaaa acagtagttt
    11341 tgaaggtcag gtacacggac tattctactg tgaccaaaag agtgacattg ccagaatata
    11401 tctataaaaa agaagcactc ttttatcaag caagccttat atgggaggaa atcttgggag
    11461 tagaaaaagg aatccgattg ttagggatta ctttgacgaa tctggacccg atgacttatg
    11521 aaaacatcgt ccttccatta tgggaaaatc aagaaatata aaaacagctt tgagagttga
    11581 aaccggaaat ccgttttctc tcccaaagct gtttttatat caagtcttgc tttttgattt
    11641 gttcataggt ttctgttgca tccgtacatt tttcccaaat cacaacttga ttggattcta
    11701 aagatttttg gacgtcaatg atccgctggt tgctacttcc gcgaaattgt agttttaggt
    11761 cacgtttgct taattcaaag cgtccgtcaa caaggatatc gatttgcgaa aggagttcta
    11821 atttatcttc tgaatcttgt agcaattcat caaaggtata gcctgtccat gaccagatat
    11881 cttttgtcgt accgaactct tttcggatac gtcgtacgac ttgcagacaa acatctgtat
    11941 tcaaaaaagg ttcgccacca agtaaagtta atccttgcac gtaatcgttg cgcaagtcag
    12001 cgatgatttg ttcttctaat tcaggtgtga atggtttgcc atatcggaaa ttttgtgcag
    12061 ctttgttgaa gcagccttcg caggcaaata agcagccact aacgtaaaga ctgttgcgga
    12121 caccttcacc atcgacaaaa ttaaacggtt tgtagtcagc aatatagttt tggctgtagt
    12181 cttttgctaa ccattctttt ggttttggat ttctcataaa aaaacttctt tcatttaagg
    12241 ttcaaagata tagctattga agataaaatc tactcgacca cataaataag aacaaactga
    12301 cctaaatcca ttgttcaaga aagcttgttc ttattttgat agagggcgtt caaagataca
    12361 aagccatcga aagagggtgg tgaaaaaaga cttgtcatag cctcttattc tgaataaacg
    12421 gtgttcaaaa gcttgcatct gcagtaataa gcccaactaa acgaaaaata tgaggaacta
    12481 tttttgttta gctattctta ttacttgatg caggacagct ttttcacaac ctcatttcat
    12541 gtgtttcaca cgtgaggaaa tttctttatg gcggccatgt accatcggac gagcttgcgg
    12601 atttcctaaa tagccacaag tacgtttgac tacatcacat gtttttggat catgatttcc
    12661 acattctgga cattcgaacc ctcgttctgt cggtttgaaa tcaccttcga aaccacattg
    12721 atagcaatga tcgattggcg tattcgtacc aagatacccg attttgtcat atgcaaaatc
    12781 ccatacagct tctaaagctt tcggattttg ttggagcata gggtattcac agtagtggat
    12841 gaatccacct gaacaatatt ttggataatc cttctcaaat tccaattttt caaatggcgt
    12901 tgggttttta cgcacatcat agtggaaact gtttgtataa tattctttat ctgtgatatt
    12961 ttcgatgatg ccgaattttt ctgtatccaa acggcagaaa cggtcagtta gactttcact
    13021 tggagttgaa tagacactga aatgataacc atattcatcc ccccatgcat ctgcattagc
    13081 ttttaattct ttgacgatat caagtgtgaa agcttttgct tctgggtttg tttcccattc
    13141 gccaccgaag aaagctgaag caacttcata caatccaatg tagcctaacg atacagtagc
    13201 tcgacggttt ttgaataatt cattgacaga atcggtacgt gaaagacgtt tcccgaaagc
    13261 accatacata tataaaattg gcgcatttgc tggaatggct tctttacaac gttctacacg
    13321 atagagaagc gcatctttca tgatttctaa gcggtcagac agtaactgcc agaatttatt
    13381 ctgatctcct tttgattcga tcgcgattct tggcaaattc aatgtaacaa cacctaaatt
    13441 cattcgtccg acatttactt cttgaccatt ttcgtctttc cacccttgca agaatgaacg
    13501 acagcccata ggtactttga aacttccagt taattcaacg attttatcgt aattcagaat
    13561 atcaggatac attcttttcg ttgcacattc caatgctagt tgtttgatgt cgtaattagg
    13621 atcttcaggg tttaagttca cacctttttt caaagtgaag atcagtttag ggaagatagc
    13681 tgtccgcttt tcactcccta atccattgat tcgaatctgt aaaattgctt tttgaatttc
    13741 tctttcaaac cagttttctc ctagtccaaa tcctaatgaa gtgaaaggag tttgtccatt
    13801 tgaagtaaat aaggtattga tttcatattc caaactttgc atagcatcga agatatcttt
    13861 tttggttttt gcttttgcat attctttttg gcgttctggc gaatcgatcc attcttccgc
    13921 gtcttttaga tgttttttat agttcaattc tgcaaaagga gcaagcaatt catctgttcg
    13981 gtcggcagaa cagccaccat attgacttga cgctacattg gcaataatct gtgagatttg
    14041 tgctgtggcg gtctggatag attttggcga ttcgacttcg gcattgccga ttttgaaccc
    14101 attgttcagc atgcctttga aatcaatcaa gcagcaattc gtcatcggtg tataaggatg
    14161 ataatccaaa tcatggtaat ggatatcgcc tttttgatgc gcatttgcta tatgtggtgg
    14221 caacattttc agtccgattg attttccaac gatcccggct gtcagatctc gctgtgtatt
    14281 gaaaacatta ctgtctttat ttgcgttttc gttcacgaca gtttggtcct tgttgattag
    14341 cttttcaatc gaaaagttga tatctgttgc tttactgcga gcaaaatctc gtttggtacg
    14401 ataattgatg tactcttctg ctaattcata ttcgtttttt tctagtaata tatgttccac
    14461 gatgttttga atctcataga ttttgacatt ttcagcaaac cggcttttga tttcttggac
    14521 aaccaagtct gcaatcattt gaattttttc ataagtaagt ggtgataatg agccgtgaat
    14581 tttcttttct gctttgatca gtgcttcatt gatcttttgt tcatcgaaat caacatggcg
    14641 gccatcgcgt ttgatgactt tcatctggga tgtctctggc tgctgagtaa cgacagtttt
    14701 ttcttcataa tttactttca ttatcttcaa ctcctgctaa attgttcttg tctatcataa
    14761 aacaaaatcg gggaaggagc aactaaatat cggctatata tagtattcaa tgtgctgtat
    14821 aattggattt ctatactata tgtagtgtat attttattgt gaaaaaaggt gaaaacttgt
    14881 agctggtgga tttgtttttt gcatatttaa ttttgctgaa taaaataatt gaataaaatt
    14941 ttttattcag tccaagtaaa agtgacaaaa acataagagc aaattattcg tagatatgct
    15001 actatgcaaa taaatagaaa tgaaaaatga aagaggagga gaagaattgg aaaagatgat
    15061 cgaagtgaaa catctaagta aaagctacgg cagcaaactt tctcccgtca aagccttaga
    15121 taatatctca ttcacagtag aagaaggcga gtttgttggg attatgggcc caagtggtgc
    15181 aggaaagaca acgctgatga atatcttgtc cacgatcaat ttgcctacga tgggcagtgt
    15241 ctcgatccaa ggaaaagaaa tcacaaaaat gaaaaacagt gagctcagtg actttcgtcg
    15301 aaaaaaacta ggattcattt ttcaagagtt caatttgatc gataccctaa atgcaaaaga
    15361 caatatccta ttgcccctag cagttgaacg gatgactaaa gaggaaatgg aaaagagagt
    15421 aagacatgta gctcaattgt tgaatataga agaactgctc aaacggtatc ccgatgaact
    15481 ttctgtcgga cagagacaac gaattgcagc tgctagagca cttgttgttc agccacaagt
    15541 gatttttgca gatgaaccaa ctggctcttt ggactcgaag tcggctacag aattgttgaa
    15601 ctacttgaag accatgaatc aaaaagaaaa agcaacgata ctgttagtca cccatgatcc
    15661 atatacagca agctactgcg atagaatttt atttattaaa gatggtgtga ttttttctga
    15721 agttgtcaga agagggacaa gaagagagtt ctttgagaaa gtaatagaca tgcaggcgac
    15781 gattggcgga ggaggaaaaa tgaatgctgt ataaactagc cgttaaaagt ttttttcggc
    15841 agtcacgagg ctatcttgtt tattttttca gtttgacttt atccgtaatg atctattatt
    15901 ccttcagcgc aatgacctac gatcaatctt tagtcagaag agcaagtcaa gatacttcga
    15961 tagatggtgg attgagtttt ggtggattta tcattactgt tgttttgctt tttttcgtat
    16021 tcagtgccaa ccgatttttc ttgaatagaa ggcaaaagga aataggcatc tatcagcttt
    16081 ttggtatgaa taaactgcag atatcaggta tatatgtgtt agaaatcatg ataattggat
    16141 tgttcgcctg tatttctggg atattactcg gtattatttt ttccaaactc ttttcaatga
    16201 ttttggttcg aatgatggat atggatctta ctagtccatt ttttatctcc attccatcag
    16261 tagtagatac tttatttgct tttttcatca tccttttagc agtatctgta tattcaatat
    16321 ggaaaatatg gcgctatcca atgattcgaa gttttggaga aaaagaacaa gcagatagtt
    16381 caacaatgcg cttcaggaca aggcaccgat tactcgcagt catcgggtta ctacttttaa
    16441 cgagcggata ttttggagct agtcatttta gggaaatcac ttcatggatg atcagttatc
    16501 attatcttga tacattagtt ttcttttttc cttttttaat ttttttcgtt tgtgtggtcg
    16561 gtacttattt gtttttctgt tatacatttc ggttattttt gatcatattg agtaaatgga
    16621 agatttatta ctatggtatc aattttttga taatgggtaa cacgcaagtt catttgctca
    16681 aaagttggcg tacaaattct ctgattacag tcattctcgg aatttcttta atgacaattg
    16741 gcggcacggc tagtatctct actattttat cctaccagga gaaagtttcc tccccgatgg
    16801 actatcagtt tgatgttgag acagagaaaa aaatcaagcc gattctagca gaggaagggc
    16861 agaagataac taaagaaatc accttgcatt ataaagtcat tgggagtatc tatagtcaaa
    16921 atctcgaaga acctatgttt caaactgcta atctgatcac ggaaacacaa tatcaagcct
    16981 tcagaaaagt taatccagat ttgccgaaca tttctttgaa atcacctggt catactgttt
    17041 tgcttgatca aatgcaaact ctttttcgga atatttcatt atatggatct agtattggac
    17101 tgccagataa tcaagaattg aagattcaac aaatgcttcc aagtgtatta ggtgacgaaa
    17161 caatggtata tctaagtcct gtactgattg tagatgatac cgtttttgaa aaagcaaaag
    17221 gtgtggatta tcaagtagtc aatgtagatg tatcaggtgg aaataatgaa aaaatagatg
    17281 aacgactgag caatgacctg tcgataaagt ggctcaatgc tgtttattat tcttatgata
    17341 tcgtaaatgg aagtctacaa ggagaaatta gcacaaagaa acttccggat gaccaaatga
    17401 aggaagcaca atttagccaa gaaagctctc gtttgaattt ctcttccagc tattcgaaat
    17461 tgcgtgcagt taatcggcga atcggtatca atatttttgt cacactattt gtcgggatga
    17521 tcgtgattat tgctactggt agtattttga cagtccggca attagcagaa gcagaggaag
    17581 aaaaagaaaa ttatcaccta ttgactaagc tggggattcc acaaagaagg atcagacgga
    17641 tgatcttcga gcaaaacgca cttaccttct ttccacccat gatattaggg atcactcacg
    17701 cagtatttgc aatcaatgtc ttcactcagt atataaaaac tgccgattac tggttggctt
    17761 atcttgtgag tggcttattg atccttatct atcttttgct ttatgtcatt acgagtcagt
    17821 tatattgtcg gattatcgag gaatgatata cggaataaga caagataata aatagaacta
    17881 aattttgaat accatttatt tggaataaga ggttgtgaca cgatttttgt cacaacctct
    17941 ttatttttta tattttttaa aagtacgaga aattataaag aaaaaagcag tgatttgatg
    18001 tgctattttt ataaaagcta tgtgacaaaa ctgttagtaa aaaaacggtt tctgtaagct
    18061 agacacatgt ttctttttgt caaaattagt tacactttgt gtgaggttat atgaggaggg
    18121 aatccaatga acaaatggcg ttctaatcca aaattgaatt attggggaaa attcatcatt
    18181 cgaacaattt tttatagtgc agtactgctg ttcctgattt atctgtatca ctataaaaat
    18241 attcaaggag gaacttttat ctataacgaa ttttaggagg aagagtatgg aaatcaaaac
    18301 gattattgaa gcaatcgatg attggggaat aaatgaacct gatcgagttg cttatcaggc
    18361 ggatgagacg catacttttg gtgaattgaa acaagcatca gacgcacttg cttattattt
    18421 agaaaaaaag gtagaaggcg atggaccaat tgtcgttttt ggcaatttgg agtttgaaat
    18481 gattgtctca tttctagggg ttgtcaaatc tggccatgcc tatttgccga ttgaagagca
    18541 tacacccaag gagcgtatcc tatcgatctt tcgggtggct aaaccatcta tggtcatcag
    18601 tatcggggac tggatagaag atattccttc ggttcctgtc ataacaaaaa aagaatttca
    18661 agaaatcaag aagataccag taggatttta tcccagaaat agcgtaaaag gtacagaaaa
    18721 tttttatatt atttttactt ctggtactac tggagaacca aaaggcgtac aaatcagtca
    18781 tgataatcta gtcagctttg ttcaatggac gatggaagac tttgggatca agcctggtat
    18841 gcatttctta gcccaagcgc cattttcttt tgatttatcg gtattttcta tttatcctgc
    18901 attagtttct ggcggaatgc tgaagccgtt aaataaagca gttgttcaag actttcgtca
    18961 attattcgcg acattgccag ccttgaagtt gaatgtgtgg gtttctactc catcatttat
    19021 ggatatttgc ttgatggaac ctacattcaa tggagaaaat gtgccggaat tggacatgtt
    19081 tttattttgc ggagaagaat tgacgaaaaa aactgcagaa agtctacttg atcgattccc
    19141 aaatgcacgg atatataata cttacggtcc aacagaagca actgtagcta tttcaagtat
    19201 tcagattgac cagcatgtac tagatagtta tgaccgacta ccgattggct acgtaaaaaa
    19261 agacacgaaa gtttctattg tccaagaagg taaacctgtt tcaaaaggag aaacgggtga
    19321 aatcattatt gcaggaccaa gcgtatcaaa aggttacctg aacaatcccg aaaagacagc
    19381 tgctgcgttc ttttgtcaaa atgacttagc aagttaccat acaggggatg caggtagact
    19441 agatgaatat gggcttttat tttatgaagg acgtatggat cagcaggtca aattacatgg
    19501 ataccggatt gaactcgggg atatcgaaca ttatctgctc caagacaacc gaatcaaaca
    19561 agctgtcgtc gtaccaaaat accaaggaac aaaagtacag cagctggttg cttttgtcgt
    19621 attagaaaac aaaacgaaac agccggattt tcagttgact aaatctatca aagaacaatt
    19681 actagaagtt gtaatggatt atatgattcc tcagaagttc cattatgtcg atctgctgcc
    19741 tcagacagtt aatggaaaaa tcgatcgaaa aaaattaaca gctgaggtga atcctgaatg
    19801 atgttacctc atatgatccc ttatgcagag ccgttttatt tttttctttt agccattgcg
    19861 cttgtaccga tcgttctatc tttattgata tgggaaaaaa gattaccagt ttatcaatca
    19921 gcagtgacac ttttcttttt atttgtcagc tttggaggta attattggca acagggagcg
    19981 gcgctgattg ggtatgtttt gtggcagacg atacttgtat ggggctattt tgtttatcgg
    20041 caaaggaaaa atcagacagg atggtttttt gccgccgtta ttttagcgat aattccactg
    20101 tttttaacga aagtgactcc tttttttgaa catggaaaag catcgttact tggtttttta
    20161 ggaatctctt atttaacctt taaatctgta caaatgatca tggaaataag agatggcatg
    20221 atcaaaacat ataagccatt tcgttatatc cagtttctgc tgttctttcc aacaatctct
    20281 tctggaccaa tcgatcgcta taggagattt gaaaaagatc agctccatcc gcctgttcgt
    20341 gataaatatg ttgatctttt gggaaaaggc gtccaaaata tttttgtcgg ttttttatat
    20401 aaattcatta tcggttatta ttttggtcaa gtactgcttc ctgtagtggg acgcattgcc
    20461 ttacagcatg gtggtatttc atgggcatta gtaggttata tgtacgtgta cagtatgtat
    20521 ttgttttttg attttgctgg ctatagctta tttgcagtcg gtacaagcta catgatggga
    20581 tatcagacac caatcaattt caataaacct tttttatcat ggaatatcaa agaattctgg
    20641 aatcgttggc atatgacgct gtccttttgg ttccgtgatt atgtatatat gcgattgatg
    20701 ttcttcatga tgaagaaaaa attttttaaa agcaagatcg taatgtcaaa tatcggttat
    20761 tttgctttgt ttcttctaat gggtatatgg catggattga cgtggtacta catcgtatat
    20821 ggactgtatc atgcttgtct gatctgtctg acagatgcat ggatccgatt taagaagaag
    20881 cataaagagc agttgccttc taacaaattt acgcatggct tagccgtatt tttgacattt
    20941 caagcagtat gttttagctt cctgattttt tcaggatttt tagataaact tttttttagc
    21001 taacgaatag tgacatatta atttatataa caaaggagaa aaggaaaatg gaagaacaaa
    21061 ttttatcaat cttagaagaa ataacaggaa cagatgaagt aagaaaaaat cgtgatattg
    21121 atctatttga tgaagggttg cttgattctt tagcatctgt tcaattatta gtcgagctag
    21181 acggagagct ggatatccaa gtccctgtat ctgaattcga gcgagaggat tggagcacac
    21241 cgaataaaat cattcaacaa gcaactgcat tgaagggttg agtatgaaaa agaaactttt
    21301 tgctattttc ggcccggttg ttttagctgt tggcttactg tttgtgtttt ttacctcctc
    21361 ctatcaagta gatatctcta atccagaaac gattagacgt gcctcttctt ccatgtcagt
    21421 gaacgtgttg aaaggagatg cgataaaaaa ttatgcatta tctgaaaaac aatatattcc
    21481 tttttttggc tcaagtgaat taagcagaat cagtccattt catccgtctg ttctggcaga
    21541 aaaataccag cgaaactatc gtccattttt gctgggagca cccgggacac aatcgttaag
    21601 ccaatatatg atgatgcgtt ctgcaggaga tgctatgaaa aacaagaaag tcgtctttat
    21661 tatttctcct cagtggttcg ttaaaaatgg tgtaaaaact gattacttta atacgtatta
    21721 ttctgaactt caaacatacg actggttgtt ttcaatgaag aaagtaacgc ctgctgatcg
    21781 ctatttagct agaagattat taactttctc aaaagtaaaa gagaatgata cgttgacggc
    21841 gattctgcaa acgatcaaaa aagggaagct acctttacct gaatctttga atcagctgcg
    21901 cagccaatgg aacatgctga agagagagga tgaagtattc agcaatattg ggttgaaaga
    21961 tcgacagcaa aaaatcgatc atgagtcaaa acgattacca aaacaatacc aagaaacaga
    22021 attatccatt ttagcaaatc aaattggaga acgagaaacg acgaataatc cattcgggtt
    22081 gaaaaacgat ttttatacgc atcgtatccg agcccacgag ccagagctca aacagtcaca
    22141 aaaaaattgg gattatcgtt tttcgccaga attttctgat tttcagctgg tcttggatca
    22201 attagcaaaa aaccataacg aagtattgtt catcatccca ccggttaacg agaaatggag
    22261 cgattatacc ggactatctc aagaaatgtt acaaggattt gccaaaaaga tcaaatttca
    22321 attaaatagc caaggattca atcgaatagc tgattttgtc aatcaagcgg gtaccaacta
    22381 ttttatggaa gacacgatcc atctaggctg gaaaggctgg ttggctgcag atcaacagat
    22441 cagaccgttt ttagaagaaa atcatattac tgctagtaag tatcatcttg acgatgcttt
    22501 ttttagcaaa tcttggcaac atcaaatacc agataaacta caattaaaat aaagaaaaca
    22561 ggcgagtgtt gctcgcttgt tttctttatt tttgaatatt ctgaataaaa gataaaaata
    22621 ccaagtttgc ctgaacaaaa atcataagca atggtataat cacaatatga atatagaaat
    22681 gggggaaaac atttggatac aaaagaagaa cagctactta ttgatttaac ttctgcaaaa
    22741 ggagtacctg gaaatgaaga agaggtaaga gaagtattca gagaatatgc caaaccattc
    22801 gctgatgata ttttttatga cggtttaggc agtgtgattg ccaaacatgg tgctggcgga
    22861 ggccccaagg tctttatttc tggacatatg gacgaagttg gttttatggt aacaaaaata
    22921 accgaaaaag gattcttgga attccaaact cttggaggtt ggtgggggca agtcatgctt
    22981 gctcagcaag ttgaaatcaa aacacgtgaa gggaaaatcg tccacggtgt gattggttcc
    23041 aaaccaccac atgttttaac acctcaagta agaaataaac cttatgaaat aaaggatatg
    23101 ttcattgata ttggcgcatc aagccaagaa gaagccaaag agtggggcat acgtcctgga
    23161 gatatggtca ctccatatat tgaatacaaa cgaatgaatg gcagtaaata tttactggca
    23221 aaagcatggg ataatcgtat cgggacagct gtttctctgc gagtattaga gaatctatct
    23281 aaagaagcac acccaaatgt tttattcgca ggaagcgatg tgcaagaaga agtaggcttg
    23341 cgaggtgctc gaacaagtac acaccttgta aatcccgata ttgcttttgc tttagatact
    23401 ggtacagcag gagacactcc aggaatgaca ccaaaagaag cagattcgat tttaggaaaa
    23461 ggcccacaga ttttgatttt tgatgcatct atgatcccgc ataaaaaatt attgaatttt
    23521 gttattgatg tagcagagga aatggatatt ccattccaat atacagtgat ttctggcgga
    23581 ggaactgatg caggacaaat gcatttgaca agagatggtg ttccgtctgt agccattacc
    23641 gttcctgttc gttatctgca ttcccataca tcagtgattc atgaagatga ttatttgaat
    23701 acagtgaaac tagtcacaga attagtgaaa cggcttgata gtgaaacagt tgaaaaaatt
    23761 cggagttatg aataatgaat aatagaatac ctatttctga atatcggaaa aaagcacgag
    23821 tttctttgga aaatcaatgg ggaatcaata cttggcttgt tttcttatct ataatattta
    23881 cgagcgtcat ccagaacttt tttgacggtt tcagtccttt tccggaagaa agcatgcagc
    23941 agcacttatt gagttttcta gtcgataatt tacttttatt cgcattcaac tatgcattgt
    24001 tttacattgc gctagtagtt gtacggggtg gcagaccaaa aagtaacctt ttattttcag
    24061 ttttccaaag aaagtatttt ggttcgatac ttggcatcaa tttgatcaat tcagtcatca
    24121 actggctact caacagttta atctttctgc ccatgttttt aatcagcgga gtgagtactt
    24181 atatgcagct gcttttcagt tcaaatgcgg tacctacggg tgtcatgcca gaagattttc
    24241 ttgatatcag ctttgtttta tcgatgatag ggatgatttt tgtttcagca ctcatcatga
    24301 gtattgttgg cggtttattc caatttgctg catggacaaa gttcgactat cctgatttgt
    24361 cggttattca atgtttgaaa tatggctggt ttttattgaa agatagaatt ggtacgtata
    24421 ttttgttgca gctgtctttt atcggctggt atttgctagg agcaatcgct ttgttcatcg
    24481 gtcttttgtg ggtcattgtt tatgtgaatg tgtcagttgc agaattttat gaacaagcac
    24541 ggattgaaaa aggaggtcca gacgcctatt ttacttagca tgtaagcagt ttattcatca
    24601 aaaaaataac ttttgtggta ggataaatct ttgaggggga atgtacgatg aaattaactg
    24661 ttttaggatg cttaggagcg tatccttata aaaatcaagg gacaacaggt tatttgcttc
    24721 aatctgacgg gttcaatttg ttgatcgatg caggtagtgc tacgctgatc aaattacaag
    24781 aacaccttga tccacttgat ctagatgctg tgatccttag ccattatcat catgatcata
    24841 ttgctgacct tggtgtatta cagtattact ggcaacttca tcctaagaga aatccggaga
    24901 cattgctttc aatttatgga cacacaaaag attccattca ctttgaagaa ttgacgttgg
    24961 aaaatgtctc aaaaggacat ccttattttg aagctgaaga attaaaaatt ggtccttttc
    25021 tgattacttt tatggaaaca atccatccag tagtatgtta cgccatgcgt atcgtagaag
    25081 aaacaacggg gaaagtattc gtatttacag gtgattcagg ttatttggaa agtttcgctg
    25141 attttgcgaa agatgctgat ttgtttctag cagataccta tctttttgaa ggaaatgaac
    25201 atcatcatgc ccatttcact tcaaaagaag ctggagaact tgcgaaatca gctaacgtaa
    25261 agaaacttgt acttacacac ctacctcagt ttggtgattt agaacaattg aaaaatgaag
    25321 cgcaaaaggc agcaggcaaa gggattgata tagaacttgc tgcagttgac aaagtgttta
    25381 acatttagga ggaagaacat gatttttgta ccaaatgaaa accacgaccc aagaatcaat
    25441 ttagcaattg aacaatattt gctgcaagaa atgcctttag atgaacctat tttgctcttt
    25501 tatatcaatg aaccttcaat catcatcggc agaaaccaaa atacgcttga agaaatcaat
    25561 cgagattatg tggaagaaaa tggcattcat gttgtccgtc gcttaagtgg aggcggagct
    25621 gtctaccatg attttggaaa cctgaacttt agttttatca tgcctgatga tggggattca
    25681 ttccgtgact ttgcgaaagt aacacagcca atcatccaag cacttcatga attgggtgtt
    25741 gctggggcgg aattgaaagg ccgaaatgat ctagtcattg atggcatgaa attttctggc
    25801 aacgcgatgt atgctaccaa cggtcggatg tttgctcatg gaacaattat gttcgactct
    25861 gatatcaatg aagtagtcaa tgcattaaaa gtaaaaaaag acaaaatcga atcaaaagga
    25921 atcaaatcga tccgttctcg tgtaacgaat atcaaaccat ttcttccaga agaaaagcaa
    25981 aaaatgacaa cagaagaatt tcgtcaagaa atcttgttga aaattttcgg cgtagagtca
    26041 gtagaagaag taaaaacgta cgaattgact gatgaagatt ggaaaaaaat caatcgaatc
    26101 tctgaggaat attatcgaaa ttgggattgg aattacggga agtctccaga tttcaatttc
    26161 tctcgccaaa aacgtttttc tatcggttcg attgaagtcc atttgaatgt ctcagaagga
    26221 atgatcaaag aagcaaaaat ctttggcgat ttcttcggac taggagatat ctctgacgtg
    26281 gaaaaaaaat tggttggaca gaaatatgat aaagcatctc tatcaaaaat agtcagccaa
    26341 attgatgtga agaaatattt tggtgcgata gaaccagaag aattgcttac cttactttat
    26401 tgaaaataaa aaatagcgga atccacatat atggattccg ctatttttta tacaggattc
    26461 gggtaatttc gagagagttg aacagtgatc cgatcaatga aagtatcttt ttcctgcgtt
    26521 agtgttcctg tgtaacgatc cgctacgtct ttcatcatca ttaagtcaaa acgttcctga
    26581 tcatctaatt cttctgtgat gcctcgacta aataggtttg tatcgtaatg tgcactatgt
    26641 tcgcaacaaa tgacgaaatg ttcctcttgt tcgtgtatcg acaaataaat ttttcgatac
    26701 agtggatttt cgatggcatg agtttctcta aaggaatgct ctaaagcgtg gatcaaaagt
    26761 tgagccagat cgtcatcgtt gataggaagt tcttcgggaa gatctgcttg gaaggttatc
    26821 tgaatattcc gtttggcagc taacttctgg taacgcgcta agataagatt tgtcacctgg
    26881 tggttgcaaa aagaacacag catttcgaaa ttttttgctt cttctaatat cttttccaca
    26941 tagtttttag cttttgtttg gtccgtatgc attaaatctt gcagattctc catctcttgg
    27001 acaaattcca ggcgaagtgt atttacttct tggatatgct gtcgaagctg ctcataatgt
    27061 tgctccaaca aatcattttt gatttttaag aaatctactt gtctccgatc tttgtcggta
    27121 tttaagatgt gatcgattag attgtagctg ataataacga ttagaatgat aatatataaa
    27181 atcgtagacc aattgatcgt tgtttgatag atacctaatg ccgtttggat gtaaatcaag
    27241 caatggataa tggctaccaa gacgatccaa ggtgtacaag ctacataaaa acggttattc
    27301 ttatatgctt cgccaatgca tgcaagtgaa gtgactaaag tgcttataac actagcaata
    27361 ttagcaatcg gcattaattc aggaagttct gcaggagtaa aagttgcata aagcagaacg
    27421 ccgataaaaa tcaaaagttg gaacgtacaa aaaattccgt accatttttt atagtaaaac
    27481 attcggctcc aatagtagca gatgatgaaa gctgacgtta agataataaa taaatgagat
    27541 aaaaaagaat gaacgaatgg ttcaaaaaga ataccactta aaatatcttc tgatacagct
    27601 tcaaatccta atgccaaagc aaagcaactt aagagaataa gtgagtattc aagacgtttt
    27661 tgtttataca tcaagtagat cgtatagaaa aaggtcccta tagcgataaa aacagcgatt
    27721 atcatcgtca atacttgcgg cactgattgt gaataaataa aactaatcaa tggtccagtt
    27781 gttcctagat aaactttcgg tggcagtcca gcatagttct gataaggaga tgagactgta
    27841 agggtcaatt tttttccgat ataatttgct ggtaaatcta tcacagccaa tgagagtccg
    27901 ggattgcttt cttcctgata atctttccgt tggtataaaa tcttttcgcc tagatataca
    27961 gtaatccatt gatggtttcc tttgattagt aaatttgctt cgtccaacgg tttttcaagt
    28021 gtcgtttcca taatgaatgt ttcgtcttta tccacggtag gaatcgtacg agtgtacttg
    28081 ctgtcaaact taaaatcagg atcatctttt gtatagaaaa cccacgaatc ttttatttct
    28141 ttcatttgat tgctgtccgc aggagaaaaa ataaaataaa agaaataagc aattcctgtt
    28201 aaaaataaca atgataatat aatgattatg ctccacttta tacgtaaata aaaagattct
    28261 tttttattat atatgttatt aaaaaaagac ataaaggtat tcctttctaa aacttgtaat
    28321 gttagggtaa acgtttttta aactaacgtc aattatttat aataaaaaat gagataaaga
    28381 tgaggaaata gaattagaaa catataatta ttaaagggaa acatttttac agctataatt
    28441 tgaaaaaaat ttatcgttat tatatgatta agaaggattg aataaagaaa gaagttgaaa
    28501 acgatacctg attacaaaaa aataactttt ataagttcta taacgttgat tatattttta
    28561 attcttattc ttctcgtcgt cttggttccc cgaatttttg gtttgactcc ttatattatt
    28621 gaggacaaag aaatgagtcc atattatgca caaggaaatc tgatatatgt acgaccgaaa
    28681 aatacaaatg agattttagt cggagatatt attacatatt atgaaaattc aggaaaaaaa
    28741 atctcgacgc gtcgcgtaat agccgttgaa aacaaccacg aagattttta tacaaaagct
    28801 gatacaaaga cttatattga gcctggagta gtaaaaaaga gaaatattat cggtagtccc
    28861 atctttcaga ttccatatat tggactagtc ctttcaaaaa cggcatttgc atggattcag
    28921 gggttattct ttacgattgc tttttgccta acaggaatca cagcatggaa tacctttgta
    28981 gagttgaaaa agaagcggag acgaacacag aaatttttgt aagaatgtta aaaacataaa
    29041 tgtattccag agatccaacg actcttctga acatgaaaca cgagaaaaca ctcatgcagc
    29101 ttgtagattt agtcagttgt tcgtataatg tgtgaagagg aggagtagta tgcgcaatga
    29161 aatgatgcat cgtcctgtag tcaaagaaga actattggat tttatgcgta cccagcaaaa
    29221 aaaattaccg ggtgagctag gcaaactgga agaagaagct catgtagcag aagtaccagt
    29281 tattccccat gaaacagttg tatttttgaa atttttactt ggacagcttc agcctgaacg
    29341 aatcttggag atcggagcag ctatcggttt ttcatcaagt gtcatggcca cgacaatcgg
    29401 agaaaacgct catgttacga caattgatcg tttcgatgtc atgatcgaga aagctaaaaa
    29461 aaactatgaa cgtctaggac tgacagataa agtgactttg cttgaaggtc aagcagcaga
    29521 tatcttgccg gagttgtctg gtccgtatga ctttattttt atggatagtg caaaatcaaa
    29581 atatattgaa tttttacctg aatgcttacg tttgttaaga aaaggtggcg ttttgatggt
    29641 agatgacatc ttccaaggag gaacgatctt gctcccagat gaagagattc cccgtggcaa
    29701 acgagctatc catcgtaaat taaatgaatt tcttcgtgtt gtgatggatc atcccgattt
    29761 gacatcgaca atcttgccat taggtgatgg tgtgatctta atgacaaagg aatcagaaac
    29821 aatcgagttg tgatacatga ttatggcagc ctgtagataa tcgattgaat cgagggctca
    29881 attcgaactt gatgtaaaag tttctatatt tgtaagtcaa ttaaaggata caggaaatct
    29941 aaataaacag gagtagcatg aagtcaatgt agctcctgtt ttcaattgat aaccttagaa
    30001 cagctgaagg agaatgtctc gttttaaaag attgatttag tagttgggcc aaaataattc
    30061 attatttgct aattaaataa ctatttcgta ttgacagaag caatttaatt atctataatt
    30121 attgatgttc cgctgattct tgtcatagta gctcagctgg atagagcatc cgccttctaa
    30181 gcggacggtc gggggttcga atccctcctg tgacgctaaa aaaccgttag aaactgaggt
    30241 ttctaacggt ttttctttgt tttttgtgcc aatattgttt gttttcaaag gcaaataaaa
    30301 taaaaaaaca tccttatcga gtacaattat aaggagcaaa aaaataaaac tcatcagaaa
    30361 agaggaagaa gatatgacga tactatctag aattttagtg actctagttg caattgaatt
    30421 tttttatatc atgtatatcg aaacggtgag aactgattct gacacgacaa gtagggtttt
    30481 taaaatgagt aaagaagagt taagcagaaa atctgttcaa acattgttta agaaccaagg
    30541 agtgtacaat ggactattag gagtgggact actttatgga gcttatcttt cttccgcatc
    30601 taaagaaatc acaagtatgc tattaatatc tatttttttt gttgcattat atggcagttt
    30661 gactagcgat cgttggattt tactcaaaca aggtgggcta gctctgttgg cattgctttc
    30721 tattttctta taaatataga aaaaataaga atttatctaa caggaaactt tgtgaaaaat
    30781 attactttat ctcatgaaat atgatattct aatgaatggt attttagaaa atttcatgaa
    30841 aaaggagtta gataggtgtt caaaaattat gtttcctcac taaaaaaaga attttcaggt
    30901 tatgatcttt caagatttca gaaagatctt ttggctggga tcacagtagc tgctgtagca
    30961 ttgcctttag ccttggcttt tggggtatca agcggagctg atgcggccgc aggtttgatt
    31021 acggctatca ttgccggtat cgttattggc ggtttatcag gcggttttta tcaaatatca
    31081 ggtccgacag gtgcgatggc agctatcttg atgtctattg cggcaacaaa gggaatgcaa
    31141 ggtgtattgc ttgctacttt tttagcgggt ctatttttac ttgcagcagg acttttccga
    31201 ttaggaacac tgacttcttt tatcccagca cccgtaatta cggggtttac ttcaggtatt
    31261 gctatcatta ttgcattagg tcagatcgat aatttatttg gtgttcactc ggaaggtaca
    31321 aacgttattg aaaaactaag tagttatcaa acgataggat ttccgataaa cacagcctct
    31381 ttgatcatgg gagggctagt cattttaggg atgtttatct atccaaaaaa atgggcgaaa
    31441 cgaatgccta gttcgctgct ggctatcatt ttggcaacag cagttatgct attgttccat
    31501 ttaccagtag caactgtggg taagattcca caaacattga tcagtagtaa tcgtttgaat
    31561 atgggagatt ttagcttttc tgccttgcaa caagtggcag ttccagctat tagcattgct
    31621 ttattaggaa tgattgaaag cttgctatgt ggtgcttcgg ctggccgaat ggcaaataag
    31681 cccttagata gcaatcaaga attaatggcg caaggaattg gaaatctgtt gcttcctttc
    31741 tttggcggga ttcctgctac tgcggccatc gcccgtacaa gcgtggccat caaatctggt
    31801 gcacaaacta gattagctgg tatgatacat gcagttgtct tactgctctc catgcttgtt
    31861 tttgcgccag tgatgtcaca gattccaatg ccagcacttg caggtgtttt gattgtgacg
    31921 gcttggcgta tgaatgaatg ggaaacgatc aaggagctat ttactaataa atattggtca
    31981 gcagttctat tgtttatcct tacgatgatt tgtacagtta tctttgattt aagtattgct
    32041 atcgtgatcg gggtgatcgg cggctgtgta ttttttgttg tcaaaagcgc tgctatcacg
    32101 atttcattag aaacgatcga ttggcaaaga atggggctgc cagaaacgga aaagctgaat
    32161 aactggacag tagtgtatat tagtggtcca ctgtttttta tgtcttcaga aaaactcaaa
    32221 aataaattgg aagaactaaa tcatcacgat ggtgtcattt tctccatgcg aggcgtacca
    32281 agtattgatt tgacggcacg tgcagttttt gatgaatttc aagaaaaagc agagcaaaga
    32341 ggacaaacga ttatctatac ttccttgcag ccagaagtag aaaaacaact ttctcacttg
    32401 tgggaacata cattcacgca tcaacatcaa acagttgctc atgcgttgac agctttacat
    32461 gcgaaatata ctgcaattag ccattaaaac aatgaaaaag aacagacaag actgtaaact
    32521 gaagatttta tggtcttgtc tgttcttttt aatattgctg taaaagtgaa gctagtactt
    32581 gccagtcttg atggataaca ggtacaaggg agcgattgta gaaaatagct gctggatgaa
    32641 aaagtggaag aattcgatag tttttttctg accattcgta ggcatccttt tgttctgtta
    32701 attgtaagac aggccctgta tagagttgac catgattttt ggtaaccgta tattctttgc
    32761 caagtaaacg ttgaagacca atgtttccaa gtgtcgcaat taattttggc tggaccttca
    32821 tcagttcgta atccaatatt ggtgcatgcg caaatacttc ggaacgagta ggtgtccgat
    32881 ttggataaac agtttctgac tgttgtgtcc gtttattgat ccgatgagtc acgcgatacg
    32941 ggcggctcct tacagcactg gtgatataca cctcttctcg tgttaagcca gttgaagaaa
    33001 gtgcctgcat caattctttt ccagcttgac cgctaaaagg aacatgcgta tcaatctctg
    33061 ttttccctgg ggcttctccc acgagcatca gtttaggatg aagaggtcct tgcccagcga
    33121 taaatccttc tacaggacgg ccgttcattc ttaaagaagc ttcttgtatc agttcttttg
    33181 ggtattccat ttatgctcct cctttttctt ttattataag aataaaagaa acacaatgcg
    33241 actattttat ttggtagaac tgtgaggata gaaaagcaga agtattgctt gaatttttgt
    33301 tttacttttt gattttaaat ttagttgact aacggttgct gttctggtaa gctagtactg
    33361 ttgtaattgt ggactccaca gctacaaccg ctcagaacaa gtaataagca cagcatatgc
    33421 tgtcacttcg gatggcgagt ctaagtatat cagaaggagg tgctttatag catgtacgca
    33481 attattaaaa ctggtggtaa acaagtaaaa gttgaagtgg gacaagcaat ctatgttgaa
    33541 aaacttgacg ttgaagcagg agaaaaagtt gtttttgacg aagttatctt agtaggcgga
    33601 gaatctacaa aagtaggcgc tccaactgtt gcaggtgcaa ctgttgaagg tactgttgaa
    33661 aaacacggca aacaaaagaa agtcgttact ttcaaataca aacctaaaaa acattcacac
    33721 cgtaaacaag gtcatcgtca accatataca aaagtggtta tcgacgcgat caacgcttaa
    33781 tagcgtttga gaaagaaggc ctatatatga tcaaaggaac atttaagcga aatgacgcag
    33841 gtcagatcgt ttcattcact ttgaccgggc atgctgatgc tggtccttat ggaagtgata
    33901 tcgtttgtgc gggtgtttct gcgttagcga tcagcactgt caacgggatt gcctcattag
    33961 ccggttttga gccgattgtt gaaatgaatg aagaagaagg cggttatctt tataccgaag
    34021 tcacttcagg aatgacacaa gaacaaaaca atattgctga gattctatta gaaaatcttt
    34081 tattaggatt acaatcgata gaagctgaaa actcagaata tatccaaatt aaaacaatta
    34141 atgataaata ggaggtgcag accatgttgt tatctatgaa tctacaatta tttgcccaca
    34201 aaaaaggtgg cggttctaca tctaacggcc gtgactcaga atcaaaacgt ctaggtgcta
    34261 aacgtgctga tggacaaaca gttacaggtg gatcaattct ttaccgtcaa cgcggtacaa
    34321 aaatctatcc aggtgtaaac gtgggtatcg gtggagacga cactttattt gcaaaagttg
    34381 atggcgtagt acgtttcgaa cgtaaaggcc gtaacaaaaa acaagtgtct gtttatccag
    34441 tagctcaaga agcttaattg aatcttatga ttaaagaggt tgtgacaaga cttttgtcac
    34501 agcctttttt gtttataaaa ataaaagttg tagtggaggc tacgaagttt actttgtctt
    34561 tttacactta atattttttt gccaatttgg aatcaggatg ttttgataac tatcgttttc
    34621 aattgatgga agctatattg tttgctatca gtgaaataat tatatggtaa acagtgttct
    34681 ttttcttgtt tccattataa aaatcgtatc gggatttttc gttcttttaa attgaacatt
    34741 tctatctatc cgcgtgttat caacaaaaca aaatcaggta aatagttgac gaaacagaaa
    34801 taaatacata gtattatgtt ttgaagccta agtacaatag tctatagaaa aggagcgata
    34861 ccattggcaa aacgcattga ccgaatttat atgtatgtca aagaaaaaac cgctcattta
    34921 acaaaggcag aatatgatca aggagtaaca acacaggaaa ttgctgaagt gttagggatt
    34981 cagcgaacaa atagcagtaa agatctgaat cagctagtgc gcgaaggaaa actgataaag
    35041 acagacggac gaccagtacg ttacatatat caaacactcg ttacgcacgt aaagccagtt
    35101 acaaaacatg tagtcagtta caaagaaacg ccggtctctg aagaaaaaaa tacactccaa
    35161 atagatacta aggatatctt tgcaaaaatc attggagcta acggcagcat gaaaaattca
    35221 gtggaacagg caaaagctgc gattttgtat ccgccaagag gattaaattg tttgattaca
    35281 ggacctaccg gatcaggaaa gacttatttt gcacacgcga tgtatcattt tgcaaaagcg
    35341 catcatgtaa tagatgagga aacggaactg attgttttca attgtgctga ttatgccaat
    35401 aaccctgagc ttttgatgag tcatttattt gggtacgcaa aaggtgcttt tacaggcgca
    35461 gatgaagaaa aaatggggat tatcgatcaa gcggatggcg gtatgctgtt tttggatgag
    35521 attcaccggt tgccgcctga aggtcaagaa atgatctttt actttatgga tcatggaact
    35581 tacagtcgtc ttggggaaac aacgaaatcc catgaagcaa atgtacggat cgtaggtgcg
    35641 accacagaag acccaggttc ttctctatta gaaacttttg ttcggcgtat cccaatcaat
    35701 atcaaacttc cggcttttga aaaacgtcct gcaaatgaga aagtcgattt agtaaaaatc
    35761 atgattgccc acgaagcaaa ccgcacgcag cgaaagattt ctttgacaga agatgtagca
    35821 aaagcattga tcggcagtgt gacctatgga aatataggac aattgaagtc aaatatccag
    35881 ttagtatgtg cacgaggatt tctaaatcat atgcaatcac ccgaaatctc gatcacaatc
    35941 gatgatttga cagaagggat tcgtagcgga ttgattcaat tagcaagcaa ccgcagtgca
    36001 atggcagagc tttctaaaat attggagcca aagataactg tagcaccaaa tgatacgatc
    36061 atgaaaatcc aatcggactc ctatgagtta ccttataatc tatatgatat catcggagat
    36121 aaagcagctt tactgaagtc ggatggtttg gatcaagaag caatcaatca tttcatttct
    36181 acagatatca acatccatct gaagtctttc tataaagacc atggtttttc ttttaatgca
    36241 gacagcaaat tagcagaatt tgtagatccg aaaatcatag aagtgaccaa tcagatttat
    36301 gtaatggtca aaagatcttt accatatgag tttcagcaaa actttattta tgctatgagc
    36361 ttgcatatca gttctttttt gaaaaaaatc aacttaggag aagaacggca tacgaatgat
    36421 aacatccgtg agatggcttt tgattttcca gatgaatatg ctgttgcgaa agaagtgcgt
    36481 agttatatcg aaacgtattt tcaagtggag attccagaaa gtgaagacta ttacttgacc
    36541 gttctactcg tctcgttacg ggcaaatcaa gcaagcggca gaatcggtgt agtcgttgca
    36601 gctcacggaa acagcacagc aagcagtatg gtccaagttg tccaacagtt gttagatgca
    36661 gagaacgtac gagcagtaga catgcctttg gatatggatc cgaaaacagc attggaccga
    36721 attgaacgca atgtacaaga agtagatgaa ggaagcgggg tcatactctt agtagatatg
    36781 gggtcccttg cgtcgtttaa ttcgcaaatt caaagagaca ctggaattcc agtacgtact
    36841 gttgatatgg taacaacttc tctagttttg gaaactgtac gaaaggtctc agtattaggg
    36901 acagatctag atatgttgca tgaatcattg aaaaattttc gaggttacgc agaaatatct
    36961 tctgtagaat ctgttcagga agcaaaacct attcataaga aaaaagctgt tttggctatc
    37021 tgcgcatctg gcgaaggtac agcacaaaga atcaaagaat tgatcgaacg agcggtaaac
    37081 aagcgtcaag aaacagaatt agaagttcta gctttgtcga ttgtggaagt gaaagaagaa
    37141 ctgccagtta tccaaaaaga atatcagatc atagctacta ctgggattac tgatttgaat
    37201 atccaagccc catttattcc tttagaacgt tttattgatc aaaatatcga agtcattttg
    37261 gatcagttgc tgatggaatc agaattggag gaaactgaat tcatctccct agatgaagag
    37321 tcagctaaaa atacgtgtgt tgagtttatc tctgataact tcatctttat taatggtagt
    37381 aagttaatcg atccaatgtg gcaattttct acacaaatct ctcagacgac gggaatcggc
    37441 gatgaagaat atggattcaa aatcaaccta gtgatgcaca cagcaggaat gatcgaacgt
    37501 attattcgga atgaaccatt gactgtggaa gaaaacgaat tgacaaatac aacgaatgat
    37561 cctctttata gccaattggc agcctcagtt gttctgttgg aagatcagat aaaagtaaaa
    37621 gtgccgatag aagaaatgta ttatctgcta cgtctagtcc ataatcagtt agataaaaaa
    37681 gaatacactg tgccatgaat acacaaaaac gatacacttt tatagtgtat cgtttttgtg
    37741 tattggttaa gaatccttta aattcaatta gtgtatcgta aaaaagttgg cacgcttttt
    37801 gcttatagta gtgtgtgcta ggaggtggtt gttcacacag aaatgatagg ggtttctgtg
    37861 aagtagtgag gaagaaggag ttttgcagat ggatattcga ttggcacgta tagatgatcg
    37921 tttaatccac gggcaagtcg caacagtttg gtcgaagcaa acaggtgttg aacgtatcat
    37981 tgtgatcagc gatgtagtcg ctcaagataa attgagaaag tttctactaa aagaagctgc
    38041 accgccgggg atcacagcaa atgtaataac cgtgcagaaa atgattcaaa tctatcatag
    38101 tggattatta tcacgagcaa aagtgatgct tttattcacc aatccccaag atgttgaagc
    38161 ggttgtccgc tgcggtgtac agttgaagtc gttgaatatc ggcggtatga gttttactga
    38221 aggcaaatca atgattacca attttgtttc agtaaatcaa caagatattg attcctttcg
    38281 ttacttgaac agtcaagaag tggagttaga gattcgaaaa gtacctgctg acaggaaaat
    38341 ccttttgatg gaactactag cgaaaatcaa aaaggtgtag caagaatggt tgttggaaaa
    38401 ggtcaaataa ttctgttgta atggttagca attattaata tttttgaatt tgttacaaca
    38461 aaagtaaata ggaggtataa atatggtagg gattatccta gcaagtcatg gacaatttgc
    38521 tgaaggaatc ttacaatccg gttcaatgat ctttggcgaa caagaaaacg tcaaagccgt
    38581 gattttgaaa ccaagtgaag gtccagatga cttgagagcc aaattggaag aagcggttgc
    38641 ttcttttgat aatcaagacg aagtgttatt cttagttgat ttatggggcg gaacaccgtt
    38701 caatcaatca aacacattat tcgaagagca caaagataaa tgggcaatcg tcagcggttt
    38761 gaatttacca atgttgattg aagcctacgc atcacgcttt tcaatggaat ctgctcatga
    38821 aattgcagca cacatcattg aaacagcaaa agacggtgta aaagtaaaac cggaagaatt
    38881 agaaccggca gaagcaccga aagcagcagt tgaagacgca caacctaaag gagcacttcc
    38941 agaaggaact gtggtcggag acggaaaaat caaatatgta ttagcacgtg ttgactcacg
    39001 tctgttacat ggacaagttg cgacagcttg gacaaaagca gtgcaaccaa accgtattat
    39061 cgttgtttca gatgcagtat caaaggatga tctgcgtaag agactaatcg aacaagctgc
    39121 tcctccaggt gtaaaagcaa atgttattcc tattagcaaa atgatcgaag tagcaaaaga
    39181 ccctcgtttt ggaaatacca aagcattgct tttatttgaa aatcctgaag acgtattgac
    39241 agctgttgaa ggtggcgtag acatcaaaga attaaatgtt gggtcaatgg cgcattcggt
    39301 cggtaaagtc gttgtaagta aagtattgtc tatgggacaa gaagacgttg aagcgtttga
    39361 gaagcttgaa caaaaaggtg tgaaattcga tgtacgtaaa gtacctaatg attcacgcga
    39421 taacatggat gatattttga aaaaagcaaa agctgaatta gcaaaagcgt aataaataat
    39481 attaaaattt ataggaggct tatcatgtct attatttcaa taattttagt cgtattagtt
    39541 gcctttctag ctggtatgga aggtattctt gatgaatttc aatttcatca accactagtg
    39601 gcatgtacat tgatcggttt agttaccggt aacctcgaag ctggtatcgt acttggcggt
    39661 acgcttcaga tgatcgcttt aggttgggca aatatcggtg ccgctgtagc accagatgct
    39721 gcattagcat cagtagcatc agcaattatt ttagttttag gtggacaagg tgttaaaggt
    39781 gttccttctg cgatcgcgat tgccgttcct ttggctgttg cagggttgtt cttaacaatg
    39841 atcgtacgta caatcgctgt tccaatcgtt catttgatgg atgcggctgc tgaagaagga
    39901 aacatccgta aggttgaaat gtggcacatc attgctgtat gtttacaagg ggttcgtatc
    39961 gcggtcccag cagcagcgtt gctatttatc ccagcacaaa ctgtgcaatc attccttgaa
    40021 tcaatgccag catggttaac agatggtatg gctatcggtg ggggaatggt cgtagctgta
    40081 ggttacgctt tggtaatcaa catgatggct acaaaagaag tatggccatt cttcgttatt
    40141 ggtttcgttg tagctgcaat ttctcaatta acattgattg ctctaggtgc tttaggtgtt
    40201 gcattagctc ttatttactt gaacctttct aaaatgggtg gttcatcaaa taatggaggc
    40261 ggaagcaact ctggtgaccc tttaggcgac attttgaatg actattaatc ttgaaggagg
    40321 aaaataaaat ggcagaagaa aaaatcaaat tatcaaaaaa agatcgttta gctgttgcgt
    40381 ggcgctctac cttcattcaa ggttcatgga actacgaacg tatgcaaaac ggtggctggg
    40441 cattttctat gattccagca atcaaaaaat tatacaaaac aaaagaagaa cgttctgcag
    40501 cattaaaacg tcacttggaa ttctttaata cacacccata cattgcttct ccaattttag
    40561 gggttacatt ggctctggaa gaagaacgtg cgaatggcgc gcctgttgat gatgtagcga
    40621 ttcaaggggt taaagtaggt atgatgggtc ctttagctgg tgttggtgac ccagtgttct
    40681 ggtttacagt acgtccaatg ctaggtgcac taggtgcttc attagctatg ggaggaaata
    40741 tccttggacc aatcatcttc ttcttaggtt ggaacattat tcgttgggca ttcatgtggt
    40801 atacacaaga atttggctac aaagctggtt ctaaaattac tgatgacctt tctggcggat
    40861 tgttacaaga cgtaacaaaa ggtgcttcaa ttttagggat gttcgtatta gctgcccttg
    40921 tacaaagatg ggtatcaatc aaattcttac cagttgtatc aactgtaaaa ttagataaag
    40981 gtgcttacat cgaatgggat aaattgcctt caggtggtga aggaatcaag agtgcatttg
    41041 aacaagttaa taatggctta gccctttctc ctgaaaaggt aacaacatta caagacaact
    41101 tagaccaatt gattcctggt ttagctgcat taggattaac attgttatgt atgtggttgc
    41161 ttaagaaaaa ggtttctcca atcgttatca tccttggttt attcgtagtc ggtgttgttg
    41221 gacacgtcat cggactttta taatccataa aaatatagct cgggcgttgt cccgagcttt
    41281 ttttatatag tatactgttc ataaacaaat atctggaggg agaaaatggt tcaatcaatc
    41341 aatacaaaag tagacttggt aattgatgct acttcgttta cgggtctgac agattacggg
    41401 caaatcatga taggtgataa tggttttgag ttttaccatg cgcgagatag acgcaaatat
    41461 atccaaattc catgggaaga agtggattat gtactagcat cagtcatgct aaagggaaaa
    41521 tggattcctc gatatgccat tcaaacgaaa aaaaatggta catacacatt ttcttccaaa
    41581 gaacctaaaa aagtgcttcg tgcgatcaga gaatatgtag atgaagatag aatggtacaa
    41641 tcattgagtt tctttgatgt agtgaaacgt ggtattcgta gtatgtttaa acgcaagtaa
    41701 aaaataaaga aaacttctaa gaggaggttt tctttatttt tttactttgc agaagaaaat
    41761 atgaaatagc aaaaccaatt atttgtcccc tttaaatccc atatgctaaa attgattagc
    41821 aaagaaggag gttatattat gagttggtta tggacattga ttgttggagc cgtgatcgga
    41881 gcaattgcag gtgcgatcac cagtaaagga aattcgatgg gctgtgtaac gaatatcatt
    41941 gctggactag tcggttcaag tattggacaa gcgattttcg gcgaatgggg accaaaagct
    42001 gcaggtatgg cacttattcc atctatttta ggcgcagtga ttttggtagc agttgtctca
    42061 tttttctttg gaaaacgatc ataagttttt acaaaatagt aaactaaaag gagttgcagc
    42121 gagactgtga ctcctttttg gctttattta tttaaaataa aaagaagttt tccggaaatt
    42181 tttatttaac tgtcgtgtac gaagaaaagt ttgctataat ggaaaaaagc gtgcaaagaa
    42241 agagggagtt tcattatgat gctacgtgta gaaaaattgc gcaaaaaaat gcaagaagaa
    42301 aatttggatt catttttggt taccagtccg tacaatcttc gttacttaac gaactttaca
    42361 ggtacgacag gattagctgt tattactttg gaaaaagcat tttttattac tgattttcgt
    42421 tataccgagc aggcggcagc gcaggctcaa gggtttgaaa ttattaaaaa tgtcggacct
    42481 atttttgatg aggtagcaaa cttagtacgc aaagaagaac tgcactcatt agcttttgag
    42541 gaaacaacag tctcattttt agaatattcg gttttagaag aaatcatcga tgcggaatta
    42601 gtacctgttt cacagatgat cgaagaactc agagaagtga aagatgaaga agaaatcgct
    42661 attatcgaaa aagcatgtca tattgcggat atggcatatg atcacatcct gaaaatgatc
    42721 caaccgggca tgactgagat tgaagtcgca aatcaactag acttttatat gcgttctctt
    42781 ggcgcttcag gtgtttcttt tgatacgatc gttgctagcg gattgcgttc ggctatgccg
    42841 catggtgtag caagcaaaaa aatcatcgaa caaggtgact tgatcacatt ggactttggt
    42901 tgctattacg aagggtacgt ttccgatatg acacgtacat tcgctattgg tgatcctggg
    42961 gaaaaattaa aagaaattta tcaaatagtc ttagatgctc aattagcagt tattgatgct
    43021 gcaaaaccag gaatgactgg tgtccaatta gatgcagttg ctagagatta tatttcaaaa
    43081 catggttacg gggaagcatt tggacattct acaggacatg ggatcggttt agagatccac
    43141 gaaggaccaa acgtttcttc tcgtgcagaa aagcaatttg taccaggaaa tgtgatcact
    43201 gatgaaccag ggatctactt accaggaatc ggtggagtac gtattgagga tgacttgttg
    43261 atcacagaag atggaaatcg tgtgttgact cgttcaccaa aagaattgat catcttatag
    43321 tttcatcaat agaagaagta agcgtaaaaa tcagtgttca cagactttgt tatagaaaat
    43381 cactcgattt aatgatacac tatagaagaa cttttaagct gtaagagata ataaagttgg
    43441 gtgttttctc caattcatag cagtttgcag ctgatcggcg gcagttttct taccacgtga
    43501 tcactatgcc gcatcataga gaattcggat gtatctcgtg cttaggcaaa gggaggaata
    43561 aataaatggc agatgaaaaa aatttgattt taaacgcaaa ccaagattta ggagagatcg
    43621 tgatcgctcc tgaagtcatc gaagtaatta tcgggattgc agcttctaaa gtagaaggcg
    43681 tatacggaat gcgcggaact tttgccagca atgtcactga attattagga cgtgcagctc
    43741 acggaaaagg tgtttactta gcaaacgagg aagatggctt gaaagtagat ctttactgtt
    43801 atctagaata tggtgtttca gttcctaaag tagcaatgga aatgcaggaa cgggtcagcc
    43861 agcaagtcct tttcatgacg gatatcgatt tatctgaagt caatatccat gtagtagctg
    43921 ttgttcctga aaaattagaa caaccaacaa tcgaagaact ttttgaagac gaagaggaag
    43981 agaatgaata aagaattatc aagacacgag attcgggaaa tggcattgca ggctttattt
    44041 ccattagact tcaatgcaga cctaacaaaa gaagatgcga ttttcaacgc aatcgaatta
    44101 gaccaccgcg acatgatcaa cgaagatgaa tcagagtttg ttcctgttta tcttgatacg
    44161 ctagttggcg gtgtctgtgc aaaaaaagat gaattggata aagtgattga aaaacattta
    44221 aaaaacaatt ggcacatcaa tcggatctca aaaatggatt tggtgatctt gcgtatcgcg
    44281 atttttgaaa tgacttatgt gacagatgta ccagcggctg ttgctttaaa tgaagcaatc
    44341 gagttggcaa aaacatttag tgatgaccgt tcacgtaaat tcgtcaacgg tgttttatcc
    44401 aatgtattga aagaattaga agctggggcg taagtcctag cttttttcta tgaatcatca
    44461 taaacctgtt ataaatcggg aaaattctat ggtagaatga aaacacaaaa aatatctggg
    44521 agtggttaga tggcagaatt gattaacgga aaagaattag ctgaaaaaat gcaggcagag
    44581 attgctgaaa aaatccaaaa attgaaagat aatggtatcc atcccggatt agtcgtttta
    44641 ttggtagggg aaaatccagc cagccaaatc tatgtaagaa ataaagaacg tgtagcaaaa
    44701 gaaatcggaa tccattcatt ggtagagcgt tatcctgaga cgatttctga agacgaacta
    44761 cttgcagaaa ttgaaaaata caatcaagat cctagttttc atggtatatt ggtccaatta
    44821 ccattgccaa agcatataga tgaagaaaag atcctgttag ctatccgccc tgaaaaagac
    44881 gtagacggtt ttcatccgat gaatttaggc agactccttg cgggtaaacc ggacaagatt
    44941 ccttgtactc catacggcat catgaaaatg tttgaagctt attctatcga tccggcgggt
    45001 aaacgagcag tagttatcgg acgaagcaat atcgtcggta aaccgatggc tcagctactc
    45061 ttgatggcag atgcaactgt tactgttgct cattctaaaa ccgaaaatct ggctgaactg
    45121 gcacaagaag cagatatttt agtcgtagca ataggacgtg gacattttgt cacaaaagaa
    45181 ttcgtaaaac caggtgctgt agtgatagat gtagggatga atcgtgatgc taacggtaag
    45241 ctgatcggtg atgtgaaatt tgatgaagtg gagcctattg caggctggat cactcctgtg
    45301 ccaaagggtg tagggccaat gactatcacg atgctgatgt atcaaaccgt aaaatctgcc
    45361 gaagcttttg gagcaggtga atagatggca caagaatatt taactgtcac tacattaacg
    45421 aagtatttga aaagaaaatt tgatgccgat ccttatttag aacgcgtata tttaacaggc
    45481 gaaatatcga atttccgttt acgtgccaat gcccatcaat atttcagctt aaaagatgac
    45541 catgcaaaaa tatctgctat tatgttcaaa ggagcattcc aaaaattacg attccagcca
    45601 aaagaaggaa tgaaagtcct tgtcgttggg cgtatttcat tatatgaagc gggaggttct
    45661 tatcaaatct atgtagagca catggagcct gatggaatag gtgctttata tcaagaattg
    45721 gaagaacgaa aagaaaagct gatgaaggaa ggcctttttc aagggcctaa aaaaatgctg
    45781 ccacgtttcc caaagcgaat cgcggtcctt accagtccaa gcggagcggt tatccgcgat
    45841 atcatcacga cggtgaaaag aagatatcca attgtccaat tggttttgtt cccaacagtt
    45901 gtacaaggaa accaagcagc ggacaatgtt gtcaggaata tccagagagt agaagagatg
    45961 ggcaatttcg atacaatgat catcggtcgt gggggtggtt cgattgagga tttatggccg
    46021 tttaatgaag agcgggttgc tcgggcgatt tatcaagcta atacgccggt gatttcatct
    46081 gtaggacatg aaacagatac cacgatcgct gatttagtag cagatgttcg ggctgccact
    46141 ccaacagcag ctgcagaatt agctgttcct gttttgtcag aagagttgat gcgcattcaa
    46201 gaacggcaag ctagattgca gcaggcgttt gtacgccaga tccagcgcaa acaagaacgg
    46261 ttcgagcgtg ctcgacgttc ctatgtattt cggcagcctg aaagattgta tgaaggtcag
    46321 accatcaaat tagaccagtt gaaacagcga ttatatcaag ctacacagca aatttatcat
    46381 gcgaaagaaa aacaaacgat cgcattaagc caccaattgg aacaagtggc tccgctttat
    46441 cgagttaaag cagctcgcca agaaactgct tatctagaaa aacgcttaga agagaaaatc
    46501 caacaatata tgaaacaaca gcagcagcgt tttgctcaag ctgttcaatc tcttgatttg
    46561 ttgagtccat tgaaaatcat gggaagaggc tacagctata cgacgttaaa tgatcaagta
    46621 gtaaaaagtg tcaccgagat tcatcaaggg gatttgctga cgatccatta tcaagatgga
    46681 atcgttaaag gacaagtaca agagatagac aaaggagaat aaaaatatgg aaaatccaac
    46741 ttttgaagag tcactgcaag aattagaaaa aattgtcatg cagttagaac aaggagacgt
    46801 tccattagaa tcagctctag attcgtttaa acgaggaatg gaattaagca aacattgcca
    46861 agatacatta gctaaagcag aagagacctt gacgaaaatg atgacagaat caaatgaaga
    46921 agttgatttt gacggaaacg aggaaagctg agatgagatt aactgacttt tcagctattc
    46981 atctacctgc tgtagaaaaa gaaatcttgt cttttttaga tgagcataca acggatagag
    47041 gactctatga tgcgatgagt tattcagtga aagcaggcgg gaaaagaata cgtcccttat
    47101 tacttttaac ggccgttgct tcctttgatg agcctattga tgttcccgtc tatcaagtag
    47161 cagctgcttt agaaatggtg catacgtatt ccttgatcca cgatgatttg cctgcgatgg
    47221 acaatgatga tcttcgtaga ggaaaaccta ccaatcataa agtattcggc gaagcattag
    47281 ctatattggc cggagatggt ttgcttacag gagcattcca gctaatcagt atggcacact
    47341 taggtaattc tcctaagctt ctgctgcttc agcaattagc tgtttgtgcg ggatcacaag
    47401 gtatggttgc tgggcaagct gcagatatcg agggcgaatc gaaaaagctt tctttagaag
    47461 aattagcatt tattcatgaa agaaagacag ggcatttgat tcgttatgcc ttgctggctg
    47521 gtggcatatt agcgaagcaa cctgaagaaa tcttattgct acttcaacga ttagccgaac
    47581 atttaggttt agcttttcag ataagagatg atctactcga tgtgattggt acaacgaaaa
    47641 cattagggaa aacagctggt aaagatgaac gtatggaaaa aaatacttac ccgcgtttat
    47701 taggcctaga aaagacacgt gaggcgttgg agattgagct gttatccgcc aacaagatca
    47761 tagacaaatt agaagaggac gttttttctt ttgatggcgg gctgatgcgc cagatgatca
    47821 agcaatttga tgtggagaaa tgagggaaag aatggaaaaa gaacgcgtag atgtattagc
    47881 ggtcaaacaa ggattatttg aaacaagaga acaagccaaa cgttcagtga tggctggttt
    47941 gatttacaac gaaaaaaacg agcggtttga taaaccggga gaaaaaattc cagtgagtag
    48001 tgaattaaga gtcaaaggta aaaaacttcc ttatgtttct cgagggggat taaaattaga
    48061 aaaagcactc aaagcatttg atctatctgt tgaaggaaaa acattgttag atattggtgc
    48121 atccactggc ggatttactg atgcagcttt gcaaaatggc gcaaaaatga gttatgcttt
    48181 agacgttggc tacaatcagc tagcttggaa aataagacaa gatcctagag tcgtcgtaat
    48241 ggaaagagtg aattttcgtt atgcaaaacc cgaagacttt accttaggaa ttccagaagt
    48301 tgctgttatc gatgtttctt ttatttcact aaaactgatg ctgcctccat tacatgctat
    48361 tttaaaagaa gagggagaag tgattgcttt aatcaaaccc caattcgaag caggcagaga
    48421 agctgtaggg aaaaacggaa ttgttcgtga ccctcagacc catcaaaaag ttttagaaga
    48481 cattttgtca tttgcggcac aaggtggata tgatgtgctg gaattaagtt attctccgat
    48541 cacgggggga gaaggaaata tcgagttctt ggctcacttg aggaaagtac ccgaaagcgg
    48601 aacaatcaat tcagctatta atatggcaga agtggtttca aatgcacacg aacagtttga
    48661 ccataaataa attgtaactg tgaaaatcaa gtgagaagta tgcggattct tctttattat
    48721 accaaggaaa taagctatca ttgattaaga agaatatgct aaggagcgaa gacgtaatga
    48781 gaaaaaaaga tcggcatcgt ttgattactc gattgttgag tgagcaagat atccgaaagc
    48841 aggaagaatt tgttgaaatc ctgaaaagca aaggaatatc tgttactcaa gctacgattt
    48901 ccagagatat aaaggaactg aaactgatca aggtaccggc ttcagatggc ggctaccgtt
    48961 atagcatacc agctgaaaca aacgaagatg taaatactaa attggaaaaa ctattaaaag
    49021 acgctttcgt ggcagttgac caaatggaaa aatttgttat tttgaaaaca ttgccgggaa
    49081 atgcttcggc agcagcaaat ttgattgata aacgttacaa gaaagaatta ttttcgatta
    49141 tcaatgatga cgataatgtt ttgatgatta cacgaactga agaccaggca gttttattga
    49201 agaaagactt cctgcattac ctttaagttg aggagcgaat gtgatgctac aagaaatcag
    49261 tattactaac tttgcgatta ttccggaatt gcggctttct tttcacgaag gaatgaccgc
    49321 gctgaccgga gagactggtg cgggtaaatc cattattatc gatgctttag gtctgttagc
    49381 aggaggaaga ggttcaagcg attatatacg tcaaggagca gaaaaatgtg tgcttgaagg
    49441 gttgtttgaa ctaccaaaac aagaaggatt ctctgaactg atggttgaat tagggatcga
    49501 aacagatgag gacaatctga ttgttcgacg ggatatgtcc ttaactggaa aaaatgtttg
    49561 tcgagtgaat ggtcatatta tcacattagc taatctgaga agaatcggca gttatttagt
    49621 agatatccaa ggacaaaacg aacatcagga actgctccag cctgaatcac atcttgcttt
    49681 attggatcgt tttggagatg tagctttcca acagaagaaa aagagttatc agcaagaata
    49741 tatctcttat cgtgagctgg aaaaaagggt acgaaaagtg cagcaaaatg aaaaatccta
    49801 tgttcagaga atagatatgc tccatttcca gcaagaagaa atagctgctg ctgatttgca
    49861 agtcggagaa gaagagaaat taaaagaaga aagagaaaaa cttagcaatt atcaaaaaat
    49921 tgctgatgga ttagctgcag gttacggagc actgacgaat aatgaacaaa acagtgtaga
    49981 tggtgttggc ttggccgttt cagaactgca agggatcgcc catctagatg tggagtatga
    50041 agcaatttat gaaaatatcc aaagtgctta ctatttgctg caagatgcaa tcggcgatat
    50101 gagtcgtcag attgatttat tggagttaga tgagagtcgt ttagaagaag ttacgcaacg
    50161 tttagaatta atccgacagt tgaaacgtaa atacggcgaa tccattgagt caatattggc
    50221 ttactatgac gaaatcaccg aagagctagc ttcttctgat ttttcagaag gtcaattgga
    50281 caaaatgaaa gaggaactag agcaaaaaga gcttctactt cagcaacaag ccgcagatct
    50341 tcatgaagcg cgtaaaaaaa tcgcaaaaga attggaaaaa tccattctgc atgaattgaa
    50401 aagtctgtat atggaaaaca ctgagtttga ggtacgtttc ttaaaagaag aaaatagaca
    50461 attgaatcat gatggatttg accaaatcga attttacatt acgaccaatc caggagagcc
    50521 actgaagcct ttggtgaaag tcgcttctgg tggcgaactg tctcggatgt tgcttgcttt
    50581 gaaaactatt ttttcttctg agcaaggtgt gacaagtatt atttttgatg aagtggatac
    50641 aggtgtgagc ggaagagttg cacaggccat cgcagataaa atttctcaaa tatcgaaata
    50701 ctctcaagtt ttgtgcatca ctcatctgcc tcaagttgct gcagtcgccg actatcaata
    50761 ttatattgtg aaagcagtga ttgatggacg tacgcaaact tctgtttcag agttgaaaac
    50821 aaaagaaaga gaagaagaaa ttgcacgtat gctggctgga agtgagatta caaaactcac
    50881 actcgaacat gcaaaagaac tattgaaact cgcaaaaagg acctgagcca cctcgcagtg
    50941 gttcaggtcc ttttcagcag ctgatagcta tattttattt ttacatgaca ctcattacag
    51001 cagtcaatac aacagatcca atcgttaaaa tcagcattgt ccaaatgaca aattttgtta
    51061 ctttagcaaa agtacttgtt tgtttttttt cacgcattgt tttcaacctc gtcattaatt
    51121 agttgattca agtgtacaat cttttttcca tttccacaag agggcttttt tataaaaact
    51181 tccctttttt taaataatag gtagttacga tacaaataaa taatgttaat agaactagcc
    51241 atataaaggc atcatccctt tcagcaatag gtaacttaat gttcattcca tagatactgc
    51301 cgacgattgt cggtatagtt aaaataatcg ttaaagaagt taaaattttc ataatattat
    51361 tcagattatt cgatacgatt gctgaaaaag tctgattcat ttgttctact agttttaatt
    51421 gaatgcgaga agtagtcatt gcctgctctg tttccaccaa tacatcacgt aaatgatttt
    51481 tcaaagcatg attttcttgg aaaagtttat tttgcgctag ttctttcaat gcatctaaat
    51541 tagcagaagt cgcttctctg aaataaacga ggcttttttg gatatccatg atttgataaa
    51601 gttgtttgtt ttcagtggat acttgaagtt tggcttccaa agtatcgcat tgagattgaa
    51661 gcgtagctaa ataactgttg taagagagtg tgacctgcca aatgaattga aagaagatat
    51721 tgagattagg cgaaatatca ctttttggaa aggtctggct aaagactttc tttaaaaaca
    51781 gtggttcatg attcgtaatt gtgataagct tctttttggg cgtcaaaata atagagagcg
    51841 ggtatgtatc cacttgaaaa taaccgctag gacttgtttt agcaaaagga tattgtaaca
    51901 ataataacgc tggctttgtc aactttgatt gattgaattc ttctgtacgg gaattttctg
    51961 catcgtccaa tatggaagtg atgtaatctg acggaatttc aaattgatgt gtcagctgat
    52021 gaatctcttc ttgattagga cgttctacac aataccagat cgtttggtca gtttcctttc
    52081 cggtattacg cagtttctca ttatctaatg taaaatattt gatcatgatt ttcctcctgt
    52141 tgcctgtttc ttttattata acggataggg aattggaata catggaatgt gtttgtaaga
    52201 tatagaatat aactgcataa atttaaataa aatgaaacag ttgtgtaaag tatatacagt
    52261 atacttgaag caacgataaa aaaggagttt acgttcatgg gcacattcac accacttatc
    52321 ttgttttggt atatttaccc ggtaatcgtt ttgtttggtt gccaatttct tgtaaaatta
    52381 ttttcgttaa atcgtcgttt taaaataaaa gcccctgatt tggcgattcc ttttttgtgg
    52441 ggcggtatgc atgcattgtc tagaaataca gggacaattt ctatcttacc gtttttcttt
    52501 atttctgttt tgctaatggg aatttgtatt gctgttttcc aagcatatta ttatgaagaa
    52561 attatttatc cgcgttattt caagatgact tggcgtttgg tttttctgtt gacaatcgtc
    52621 ttgtacgctg tcttgatcat tgtgaacata ctttcttacc tatgaaaaaa atagaaaagc
    52681 tgtaaaaagt cttttaaaag gctttttgca gcttgatttt taaaaaaatg tttatgaata
    52741 aaaaaagttg ttcccggatt tcccagaaaa ggtggtagaa agtgggggat tgtggtagac
    52801 taattttagt tagtggagga atggggggct ttcaatgttg atgggtgaat tccaacataa
    52861 tattgatgca aaaggacgtt taatcgtgcc ctcaaaactt cgtgaagaac ttggcgaaaa
    52921 gtttgttctg acacgcggat tggatggctg tcttttcggc tatccgatga gcgaatggga
    52981 aaatctggaa gctaaactga acgaaatgcc gttggctaaa aaagacgcac gtacttttgt
    53041 gcgattcttc tattcggctg ccactgaatg cgaacttgat aaacaaggac gaatcaatat
    53101 acccagcacg ttaagaaatt atgcagcttt gacaaaaggt tgcgtagtca tcggtgtatc
    53161 taaccgaatt gaaatttggg atgagacacg atggcaagat ttttctgcag cagcagaaga
    53221 gaactttgac gaaattgctg aaagtatgat tgatttcgga ctataagaat aggaagaggc
    53281 tgactaatga ctgaaacatt tcaacactat acagtcatgt tgaaagaaac ggtagatgga
    53341 ttgaacatca aaccggatgg cgtttatgta gactgtacac tcggcggtgc cggacatagt
    53401 gaatatttat taagccaact aagttctaaa gggcatctgt atgcgtttga ccaagatcaa
    53461 aaagcaatcg atcacgccaa gctgcgttta gcttcttatg tcgaacaagg tatggtcaca
    53521 tttatcaaag cgaacttcag agaattagaa gaacgcttga gtgagcatgt agataaggta
    53581 gacggcattt tgtatgatct aggggtttcc tctcctcagc tagatgaagc agaacgtgga
    53641 tttagttatc atcaagatgc accgttggat atgcgcatgg atcagtcagc tcctttatcc
    53701 gcttatcacg taattaatga atattcctac catgaattag taaagatttt cttccgctat
    53761 ggcgaggaga aattttccaa acaaattgct cgtgaaatcg aacgagtacg aaaaacaaaa
    53821 ccaatccaaa caactggtga gcttgtcgaa atcattaaac aagtcatccc agctcccgca
    53881 agaagaaaag gcgggcatcc tgcaaaaaga atttttcaag ctgtgcggat tgcagtaaat
    53941 gacgaactcg gtgccgaaga ggcctcttta gaacaggcaa tcgggttatt gaaagtcaat
    54001 ggaagaatca gcgtaatcac attccattca ttggaagaca gaattgtcaa aagtatgttt
    54061 aaagaatata gtacggtaca agatctacca ccgggacttc ctgtcatacc tgacgaattc
    54121 caaccagaac tgaaagtcat tactcgtaaa ccaatccttc cttcagagga agagctaaca
    54181 gaaaacaatc gatcacgcag tgcaaagtta cgtattgcag aaaaaataag aatgaaagag
    54241 gagtagaatg atggcagaat taaagaaaat gcaagattac gaatatgatt tacctgaatt
    54301 ggtggatcag ccggaacaac aaatgcctga ttcttcttcg aatcaagaaa tcattcttcc
    54361 acaatcaccg gcaaaacgat taaagcagat ttctgctttg gaaaaaattg ctgtggcaag
    54421 cgtggcgatt gctattgttg ctttatgtgt tttaacagtt atgctgcgca caaatatcag
    54481 cggagctgaa aaagaaatca cgtcaataca aacagaaatc aatgataaaa agcaagaaaa
    54541 aacgagttta gaacaagaaa aaaacgaact ttctcgtact gaacggatta aacaaatcgc
    54601 agaagaaaaa ggattgtcta ttaatgacga caatttaagg aaagtgaagt aaatgagtct
    54661 taaaaataaa attcggcggt ttatggaaaa gaaaaatcta aatccgatga acaatcgcaa
    54721 aaaagtaggt atcattttat ttgctacgag tattggatta ttctttttat ttgccgtccg
    54781 tttctcgtat atcgttattg gaggtcatgt tgctggtacc tcattagctg aaaaaacaaa
    54841 acagttgtac caaggaagtg aagtagtaaa agcaaaaaga ggaacgattt atgatcgtaa
    54901 cggtgtggcg ctcgcagaag atgcctcctc ttattcaatt aaagcgatac tctcaaaaac
    54961 atatacctct ggggataaaa aactgtatgt tgaagaaaag aattttgata aaatagcaga
    55021 aatcctgcat aagaatcttt ctatagacaa aaaagatgca ctgaatattt tagaggacgg
    55081 cgcaaaaaaa gagctgtatc aagttgaatt tggtagctat gggaaaaata tctcccaaga
    55141 aaccaagcaa aacatcgaag cggacatgaa aaaagaaggt gtcgctggtc tttattttgt
    55201 cgaccatcag gctcggatgt atccaaatgg cgtcttttct tctcatttta ttggatacgc
    55261 agttccagac aaagatgaga atggcttagt tggtaaacta ggtttggagt ctgcttacaa
    55321 tgatatcttg agcggtaaag atggtaagat tatttatcaa aaagataatt ttcaaaatcc
    55381 tctgccaggt acagtggcag aagaagaaaa agctgtcgac ggacaagata tctacacgac
    55441 tttagacagc agactacaaa gttatttaga aacactgatg gatcaagtaa atgaagaata
    55501 tcagccagaa gaattgacag cagtcctgat gaaagcaaaa accggagaaa tcttggcaat
    55561 gggacaacga cctactttca atcctgaaac aatggaagga ttgactggaa aagatgctat
    55621 ctggcgtaat ttcttggtac aagatagtta tgaaccagga tcaaccatga aagttttcac
    55681 tactgcagct gctatcgaag aaggagaatt caacgagaat gaaaccttcc aatctggaaa
    55741 aatccaagta gaagatgcta cgatcaatga ccatgacttt ggtgaaaaag gtgtgctgac
    55801 catgcgtcaa gctctttcat ggtcaagtaa cgtcgggatg gtcatactgg aacagcgatt
    55861 aggcggacga tggtataact atttacaaaa actgggattt ggacaaagta ctcattctgg
    55921 attagatgat gaagtaaacg gtgctttacc tacattaaat attgtcgacc gagcaatgag
    55981 tgcttatgga caagcggtag gtgtgacgaa tttccaaatg atgaaagctt tcacttctat
    56041 tgcgaataac ggtacaatga ttcaacctcg atatatcagc aaagtggtgg atccccaaac
    56101 tggagaagag cgaacaactc aaacagaagt tctagggcaa cctttttcaa aagaaacaac
    56161 agaaaaagtc cgtgaatata tgagagatgt tgtcgaaagc gaaaactacg gtagtgctta
    56221 tggtgtatat agtgtacctg gatacaatgt atcggcaaaa accggaacag cccaaatcgc
    56281 ttctgataca gggggatatc aaacaggaga tacagcctac ctgtattcga ttgtggagat
    56341 ggttccttct gaagatccag actatgtctt gtatctaaca atgaaacacc ctaagacata
    56401 tgatcgaatg gcattggcta aaatcgcaaa tccattgatg aaacgggcaa tggattttaa
    56461 agaaactgaa gaagattcag atacagaaac aaaaacagaa aaagtatccg tagccgatta
    56521 tcgtaatctt gaagcggacg ttgcagcagc tgatgctcaa aaaagtggac tgcaacctgt
    56581 agtgatcggt aatggtaaaa aagtacaaaa acaatctact gccaacggtg atcagttgat
    56641 ctctggagaa aaactgatcc tatatactgg tggtgataag ctgatgcctg acgtaacagg
    56701 atggtcaaaa gcagatatca tgaagctggg gaaaatcctc ggtattgaag ttagttttga
    56761 tggagatggt tattgtgtca aacaagaact tgctccatat gaaaaaatca ctgaagacaa
    56821 acttagtttt actttagaag aataagaaga gaaataggag agaaaagagc atggaatgga
    56881 cacaagcatt gattcccatt gtcagcagtt gtgcaatgac aattgccgca atgccgctat
    56941 ttattggtta ttttcaaatg aaaaagcaag gtcaggcgat tcgtgaggag ggaccaaagt
    57001 ggcacaatag caaagccgga actcctacaa tgggcggctt agtgtttctg atcggctcta
    57061 ttttgacagg aatttgggtt ggagcttggc aaaaacagct aactcctact cttttcatct
    57121 tgctttttgt attagcgtta tacggagtaa tcggctttct tgatgatttt ataaaaatat
    57181 tcaagaaaag aaacatggga ctgaactcga agcagaaatt gattggacag attctagggg
    57241 gaatcatctt ttatcttgtt taccgttctg aaggttatcc aggtgtattg aatttcttcg
    57301 gactagatct acctttagga atcatttatg gtattttcgc tctcttctgg cttgttggat
    57361 tttcaaatgc agtaaatctg acagatggca tcgatggatt agttgctggc ttaggaagta
    57421 tctcttttgc agcttacggt ctaattgctt ggcatcagca tcagtatgat gtattaatca
    57481 tttgcttaag tgttttagga ggtttactgg gtttcttcgt ctacaacaga aaacctgcca
    57541 agatttttat gggagatgtc ggttcattag cgttgggcgg acttttagcg gcaatttcta
    57601 taatgctaaa ccaagaatgg acattgcttt tgattggttt gatctatgtt atggaaacag
    57661 caagtgtgat gcttcaagtt acttccttta agcttacagg aaaacgaatt tttaaaatgt
    57721 ctccaattca tcatcacttt gaaatgtgcg gatggtcgga gtggaagatt gatacagttt
    57781 tttggttgat cagcattgtg acttcgttga tcacattatg gatcgtttgg taggagatga
    57841 cgggaaatga aaaaaatatc aacatatgaa aataagaaag tgcttgtctt aggactcgca
    57901 aaaagcggag taagtgcggc caaattatta catgaattgg gcgcacttgt caccgttaat
    57961 gatggaaaac cttttgacga aaatccggaa gcacaagagc tgctttcttt agggataaaa
    58021 gtaataacag gtagtcatcc aatcgaattg ttggatgaag aattttcatt gatggtcaaa
    58081 aatccaggga ttccctacag tcatccttta gtagcaaaag cccaagaaat gggtattccg
    58141 gtaattacgg aagttgagct agcttacgaa gtagcggaat gcccaattat tgggattaca
    58201 ggaacgaacg gaaaaacaac gacaacaacg atgacaggac tactactgaa tgcaggagca
    58261 gatcaaggaa ttgcacgttt agcgggtaat attgggtatc cagcaagtgg tgtagcgcaa
    58321 gaagcaaaat cagaagataa aattgtgatg gaattgtcta gcttccaact catgggaatc
    58381 acagatttcc gccctcatat agcggtgatt accaatatct atgaagcaca tattgattat
    58441 catggtacaa gaaaagaata tgtcaaagct aaatggaacc ttcaaaaaaa catgacagaa
    58501 aaagattatc tgatccttaa ctggaatcag tcagaactac aagaattagc gcaaagaaca
    58561 aaagcacgcg tactgccttt ctcgacaaaa gaagtgttgg aagacggtgt gtatgctgac
    58621 gattactcaa tatactataa aaaagaaaaa ataatggaaa tatctgaact tggcgtacca
    58681 ggtaagcata atgtagaaaa tgcactagct gctatttctg tagcaaaatt gtatggtata
    58741 tcaaatgaag ctatacgaga aacgttgcat tttttccatg gcgtaccaca tcgtacgcaa
    58801 tatgtaggag aaatccaagg cagaaaattt tataatgatt caaaagcaac caatatttta
    58861 gcgactaaaa tggctctaag tggatttgaa acgtctaagg tcgtgttatt ggctggcgga
    58921 ttagacagag gaaatacatt tgatgaatta attccttcat taaaaggaat caaagcaatg
    58981 gtcgtatttg gccaaacaaa agaaaagttg atggatgctg gaaaaaaagc aggcatcgag
    59041 acaatagtaa cagccgattc tgtagaacaa gcagttcctt tagcattaga aaacagtaca
    59101 gatggtgatg tagttttatt atcaccagcg aatgccagct gggatcaata cccaaatttt
    59161 gaaactagag gaaacagatt catggaagct gtgaaccgat taaaatagaa aaagtagaag
    59221 cgagcgataa aaaatgaaaa ttttggttac aggcggagga acagggggac atatttatcc
    59281 agctcttgcc tttgtcaatt atgtaaagac tcaagaaccg aatgctgaat tcatgtatgt
    59341 gggagcaaaa cgaggtctag aaaacaaaat cgtccctgat acaggaatgc ctttccatac
    59401 cttggagatt caaggattta aacgaaaaat ttccatgcat aacatcaaga cggtccgatt
    59461 gtttcttaag agtatccgtc aagcaaaaaa aatcctcagt gattttcaac cagatatcgt
    59521 tatcggtact ggaggatatg tttccggagc ggtagtgtac gctgcagcca aaatgggtat
    59581 tccaacagtt atccatgaac aaaacagtgt accaggtgtt accaataaat ttttaagccg
    59641 atatgtggat cggatcgctc tttcatttaa agatgcggct tccttttttc ctgaaaacaa
    59701 agcagttctt gtaggtaatc ctcgagcaca agaagtagca gatactaaaa aatcagatat
    59761 tttgaaaaca tttgatttgg atccagaaaa gaaaacagtc ttgatttttg gcggtagtca
    59821 aggagcactt aaaatcaatc aagcagtatc agttttttta agtgaatata ttccttcttc
    59881 ttaccaagtt ttgtatgctt caggagatag atattatgaa gagatcctaa ataaaataaa
    59941 tcctgaaata caaaatatta gtgtccagcc ttatatcaaa aatatggcag aggtcatggc
    60001 ttccagtgat ctgctggtgg ggcgtgctgg tgcaacttcg atcgcagaat tcacagctct
    60061 ggggttaccg gcagtgttgg ttcccagccc atatgttacg aatgatcatc agactaaaaa
    60121 tgcaatgagt cttgttcacg caggagcagc aaaaatgatt gcagataatg aactaacagg
    60181 tgaatcacta agtcagacag tcaacgaaat catgggtgat gaagaattgc aaaagcaaat
    60241 gtgccgagct tctaaagaac aaggaatccc tgatgcttca aaacgtcttt atgacttagt
    60301 aaaacaaata atcaaaaaat aaaggcggtg gtcttagtga gtaagaaaaa agaaccaaaa
    60361 aaattgtctc atccgtcttc tgatgaaaac aatttgactc cttggcaaaa ggctaaccga
    60421 aaatatctgg cagaacaaga gatgaaaaac caagagccaa aaaaagaaga cataacatta
    60481 gatacacccc tttcagattc tgaagaagaa aaaacggttc aagaggaaca gcaagaagaa
    60541 agccagtccg agaaaaaaat cgaatttttt gaagagatca agcaaaagaa aaacggtgga
    60601 ccatataacg gttctttttt gaaccggctg cccaatttaa aaagtcaacg gcacaaagta
    60661 ctatttcgtc ggttagcttt gatcatcact gtattgggta taccgctgat ttttttgatc
    60721 tattatgttt ctccttatag caaattacaa gcagttgcag tatcaggcaa taagacagtc
    60781 aacagccaag aaatcatctc tgatacaaaa ttgtcattag gagaaaatgt ttggggacag
    60841 tattttcatc gatctactta tatcgatcgc cttaaaaaag cacagccaag aatcgaaaca
    60901 gcaaatatac atttcaaagg gatgaatgaa ttcgatttgg atgtaacaga atataaagag
    60961 attgctttga tcgcaaaaaa caatcaatac tacccagtga ttgaaaatgg aacagtatta
    61021 gatgaaaaag tagcaaatcc tacaaaaaat ctccctatct tggaagagtt caaagactct
    61081 gcaaaaatca aagaattaac aaagcaatac aatcagttat catcagaatt acaaaaagca
    61141 atttctgaaa tcaagtacac gccaagagca agcaataaaa atctgatcca attgaatatg
    61201 aatgacggca atcaagttat tgtaaatatc aataatcttg cgaaccaaat gaaatattat
    61261 tcgcaagtag ccaaagatat ggatgaaaaa ggtgtgatcg acatggaagt tggtatcttc
    61321 tcgcatcctt acggtagcac tcaagaaacg gaagaatcgg gccaatcgga agaaacaact
    61381 gaaagtgaag aaaatgcgga tcaaacaaac gaatcaagtg aagaggcaga aacgcaaaaa
    61441 gcacaaacac ctggatcgtc tagcacaaac ggatcatctg attcaaatga ttcaagtgaa
    61501 acaagtcaaa tagaagataa tccaagtaca actccgtcaa gtttaaatga aaattgataa
    61561 aacaacttta aaaagtaaga atgtactttc taaaaaccag taatttatgg tagaattgga
    61621 agcagtgaat atatctatta ataagtatat tttaaaacgg acgctagttg ttcgcttgta
    61681 tatggaatag taagtaggag ggaatcccat tcatggcaaa aacaggaatg tatgtaggcc
    61741 ttgatattgg aacgacatct gttaaagttg tcgtggctga atatatcgac agccaaatga
    61801 atattattgg agtaggaaac gcaaaatcag aagggattaa ccgaggcatt atcgttgata
    61861 tcgataaaac ggtccaagcg atacaacgag cagtacgaca agcagaagaa aaagcaggta
    61921 tccagatcaa aggcgtaagt gtcggtttgc cagcaaacat gcttgaagta gaaaattgcc
    61981 agggaatgat tgcagttaac ggagattcaa aggaaatcac cgatgaagat gtcagaaatg
    62041 tggcttcagc tgcccttgtt cgttcaatac ctcctgaacg tcaaatcgtg tcaatattgc
    62101 cacaagattt tactgtcgat gggttcgagg gaatcaaaga tcctcgtgga atgatcggcg
    62161 taagattaga aatgtacggt ctgttgttta caggacctaa aacgatcatc cataacatac
    62221 gtaaatgcgt ggaaaatgcc ggattaatcg tcaacgaaat ggtgattact cctttagcgc
    62281 tgactgaatc aattctttct gacggagaaa aagattttgg tacgatcgtc attgatatgg
    62341 gtggcggcca aacaacaacg gctgttatgc atgataagca attgaagttt accaatcttg
    62401 accaagaagg tggcgaattt gtcactaaag atatctctat tgtattaaat acgtcattta
    62461 acaatgcaga agcgttgaaa ataaactacg gggatgcata cccagaacgg acatcaccag
    62521 atgaagaatt tccagtagat gtgattggtc agtcagaacc agtgaaagtc gatgaacgct
    62581 acttgtcaga aattatttca gctcgaatgg aacagatttt caacaaagca aaagatgcat
    62641 tggaccaaat cgaagcatta gagttgccag gtggcgtcgt attgacaggc ggagctgcta
    62701 gtcttcctgg tgttgtggac ttggcacaag aaatcttcgg cgtaaatgta aaattgtatg
    62761 ttccaaatca catggggctg agaaacccgg tatttacaaa tgtaataagt atcgtggatt
    62821 attcagcaaa cttgagtgaa gtgtatcaat tggcaaaaat tgctgttacg ggtgaaacct
    62881 cagcagctcg tcaaatggta gttgaacaag aaacaacaaa cacatatgag agctatgaag
    62941 cacctgaaga aacaatctac gatgagccgg aaccaaaaga gtccggagaa aatgtaaaaa
    63001 ataaaatcaa gggtttcttt acaaatattt ttgattaatt catcctggga ggaataagat
    63061 atggaattct caattgataa taatatcaat gacggcgcag taatcaaagt catcggagtc
    63121 ggtggtggcg gtggaaatgc tgtaaaccga atgattgaag aaaacgttaa aggtgttgaa
    63181 ttcattacag ccaacacaga cgtgcaagca ttaaaaaatt caaaagctga aacagttatc
    63241 caattaggcc ccaaatatac tcgtggttta ggtgctggtt ctcagccaga agttggacaa
    63301 aaagcggcag aagaaagtga acaatcctta cgcgaagctt tagacggtgc agatatgatt
    63361 ttcatcactg caggaatggg tggaggaacc ggtacaggtg ctgcaccaat cgttgcaggt
    63421 atcgcgaaag aattgggcgc attgactgtt ggtgtcgtga cacgtccatt cacttttgaa
    63481 ggaccgaaac gtggccgttt tgctgcagaa ggtatcgctc gtttgaaaga aaatgtggat
    63541 acattattaa ttatctcaaa taaccgttta ttagaagttg tagataaaaa gacaccgatg
    63601 cttgaagctt tccgtgaagc agataatgtt ttacgacaag gtgtacaagg tatctctgat
    63661 ttgatcacag cgccaggtta cgttaacctt gactttgctg acgtgaaaac agtgatggaa
    63721 aaccaaggta cagcattgat ggggatcggt gtggctagtg gagaagatcg cgtagtagaa
    63781 gcaacgaaaa aagcaatctc ctctccattg cttgaaacat ctattgatgg agctgaacaa
    63841 gtacttctta acatcacagg cggtttggat atgacattgt tcgaagcaca agatgcttca
    63901 gatatcgtag caaatgcagc aactggcgat gtaaatatca ttctaggtac atctatcaat
    63961 gaagaaatgg gcgatgaaat ccgtgtgacg gtcatcgcaa caggtatcga tgaatcaaaa
    64021 aaggaacgca agtctagccg accagctaga caagcacaaa tgcaatcacc tgctcaaaag
    64081 actgtattag atatggatca agctaaacca acttcttcag aagaagaaaa cagttttggc
    64141 gattgggata tcagacgtga acaaaatgtt cgtcctagag ttgatgattc caattttgac
    64201 aatatcgaaa aaaaagaatt tgatacattc aaccgcgaag aaacaaaatc tcatggcgat
    64261 gatgaattaa atacgccacc gtttttccgt cgtaaaaaat aaaggagagg aagagcacat
    64321 gattgctgac aacttgcaga aaatcaagca agaaattcag gattcgtgtg ctcttgttca
    64381 tcgtgatccg aaagaagtta cattagtagc tgtaacaaaa tctgttgaat ccgctcaagc
    64441 tgaagaattg gttagagctg gcgttatgaa ttgtgcagaa aaccgagtag ataaattatt
    64501 agaaaagaaa caagatttat ttgattttcc tgaaataaag tggcatttaa ttggtaattt
    64561 acagcgcaga aaggtaaaat tgattgtaaa tgaaattgat tactttcatg cgttggacag
    64621 tttaagatta gcagaagaaa tccagaagcg gttagagaaa gaattatcct gttttattga
    64681 agtaaatgtt tctggtgagg aatcaaaaca tggaatttca ccagatgagc ttttaccgtt
    64741 tgtggaatct ttagctgcct gcacaaaaat caaggttgtt gggttgatga ctatggcgcc
    64801 taaagaagct tctgatgagc aaatccataa agtgtttggt acgctgaaag aattgcgtga
    64861 tcttgttcaa gcaaaaggtt atgcccatgc accatgtagt gaattaagta tggggatgag
    64921 tcaagacttt caaattgcga ttgaagaagg tgcaacattt atccgtgtag gaacagcgct
    64981 attcaaagac gaataaagga ggaacaaaaa tgccgctttt aaacagagga gcaattgcta
    65041 attttttcgg attagctgat gaggaagagt acgaatacca agattatcct gaaacaacaa
    65101 aaaaggctgt acagccaact aaaaaaaata caacacagaa tcaatcaact cattctgtat
    65161 ctgctgcgaa tactcatgaa aaacaaaaaa cggcgacttc acgtcctgta acgcatgaaa
    65221 gtattcagcc ggctgaaaag aaaaaagtaa ttgaactaca tcaatcacct acaaaaactg
    65281 tagcgaaaga aagaacagct tcaagaacct ctacacaacc taaagcaaat cttgctggaa
    65341 aaatcactgt aatggaacca agagcttatt ctgaagctat ggcgattgct aaacgaatca
    65401 ttgctggaga agcaacctta gtgaattttc atttagtaga agaaaaacaa gcgagaagaa
    65461 tcgttgattt tctaacagga acagtttatg cactagatgg agatatcaaa cgtgttggag
    65521 atgagatttt tctttgtaca ccgactggga tggagattga cagttcaacc gctcaatctt
    65581 ttgcagatgc gaatttgttt gatctgtaag aggaggaact acgatttata ctattgcact
    65641 attacttcat ttacttcaac gggcagccta tttctatagc attatcttga tggtatatgc
    65701 attgctctct tggtttccag gcgggtatca atcagggcta ggacgattat tagctaagat
    65761 ctgcgaaccg tatatcagtt tgttcgatcg tctgcctttg aggattggac ccttggattt
    65821 cacaatttta ggggcaatcc tgatcttgaa cttagccgta caaggtttag cacagatcgt
    65881 tatatggata actgtccatt tagtttattg attgaagcaa caaaatcttt attctcagaa
    65941 tagataggag gggaaaattt tggatgcaaa tgtataccag cactttcggg cagatgaacg
    66001 tacatttatc gattcggtag gcgattggat tgaacaggtt caaggacaat atgcacccta
    66061 tctgaccgaa tttcttgacc caagacaatc ctatatttta gaaacactga ttcgacaaag
    66121 cagtgatttg cgtttcatgt tttatggcgg atatgaacag gcagaaagaa aaagatgttt
    66181 gattttcccc gattattacg aacctgtaga ggaagatttt gaaacagcat tgtatgaaat
    66241 ccattatcct agcaaatttg ccagcctaag tcatgggaaa atccttggaa ctttagtggg
    66301 aacggggatc aaacgctctt actttggcga tatcatctcg gacggtgatc gctggcaagt
    66361 atttattgca aatgaggtgg atcattttgt tgctgcacaa gtaacaaaga ttggaaaggt
    66421 tgctgtacgc ttggaaccaa tcaaatacac agaaatgatt atgccgaaag atagctggac
    66481 acaagaaaga ggaacagtta gttccatgcg actagacagt gtgatctctg agatttataa
    66541 tatttccaga cagcggtcca agcagctgat cgaagctggt aaagtaaaag tgaactggac
    66601 agaaaataca agacctgatt ttgtacttga actattagat attatttcag tcagaggtta
    66661 tggcagagtc cagatccaag aacttgaggg gaagacgaaa aaagaaaagt acagggtact
    66721 gttcggtgta ttacgtaaat aaaataaaaa ggtggtttac tcatggcttt aactccatta
    66781 gatatccaaa ataagacatt cccgacaaaa atgcggggat ataatcaaga cgaagtagat
    66841 gatttcttag atttagttgt tcgtgactat gaggaattga ctcaaagaaa tcgagaatta
    66901 gaaaaagcag tcaaacattc tgaagaaaaa cttgagtact tcaatgaatt aaaagatgct
    66961 ttgaaccaat caatcatcgt agctcaagat acggctgata aagtaaaaac aagtgcaagc
    67021 aaggaatccg aagtgatcgt tacttctgca caaaacaaag cagatgagtt agttgcgaac
    67081 gctgaaaaac gtgcacatca attaacaact gatgcagaag aaaaagcaag aaaaatcttg
    67141 acagatgcaa cagaaaaagc aagacagctt gcgacagaaa ctgaagatct taagaaaaaa
    67201 acacgagtat tccatcaacg tatcagcttg atgcttgagt ctcaattaga acaagtgaaa
    67261 agtccggaat gggatgaaat tttacaacca ttctccagct acgtgactga cagccatgaa
    67321 gtgatcaaag aagttttagc aaaacagctt gacaatgaaa atgatactga tgtaaactca
    67381 gacacaggag tcatagaagc acctatgacg gcatttgata cacaggctat tgatatctcg
    67441 atcgtgcctg gtgaaaatga aaacgagtag aacagaaaag cactttgtta ttcttaaagc
    67501 gagccgatga gagtggaagt tcggtaagac actgcatggt gaaagatcct ctacttgaga
    67561 cctgcttttc cgaaattaga gtaagaagca gcggctgacc tcgttattgg tcacagagag
    67621 tttgcactag atagatgcaa ctaaatttgg gtggtaccac ggcatgttcg tcccttgcgg
    67681 ctgaacatgc ttttttgtgt ccggaaaaca aataaaggag tgtatggttg aatgaaaatg
    67741 aaagaaacct tgcaattagg aaaaacaaaa ttccctatgc gtggtaattt gccaaaccga
    67801 gaaggcgagt ggcaaaaaga atgggaagaa aatcgaatct accaaaaaag acaagagttg
    67861 aacgaaggaa aacctagctt tgttcttcat gatggacctc catatgccaa tggtaatatc
    67921 catttaggac attcattgaa taaaatcagt aaagacatta tcattcgttc aaaatcaatg
    67981 tctggctatc gtgcaccata tgttccagga tgggatacac atggattgcc gattgaacaa
    68041 gtattggcta ataaaggcgt gaaacgtaaa gaaatgtcca tggcagaata cttggaaaaa
    68101 tgtcgtgaat atgcactttc ccaagtagat aagcaacgag cagatttcaa acgcctagga
    68161 gttgcaggag attgggaaca tccttatgtt acattagatc cttcttatga agctgcacaa
    68221 gtacgcgtat ttggtaaaat ggctgaaaaa ggctatattt acaaaggatt gaaaccaatt
    68281 tactggtctc cgtcaagtga gtcttcttta gcggaagctg aaattgaata taaagatgta
    68341 aaatctccat ctatttttgt agctttcaat gtcaaagacg gcaaagatat tcttgatgaa
    68401 gatacagctt ttgtcatttg gacgacaaca ccatggacat tgccagcaaa ccaaggtatt
    68461 gctgtaaatc caaaatttac gtatgtattg gtggaagccg atggccgtaa gtttgtcgta
    68521 gcgaaagatt tgctagaaac agtccaatca gaaatcggtt gggaaaatgt aacagttcta
    68581 aaagaatttg ctggacaaga aatggaatat atgactgcaa gtcatccatt ctatgaacgt
    68641 gaatcacttg tgattttagg cgatcatgtt actttagacg ctggtactgg attagtccat
    68701 actgctccag gacatgggga agatgactat atcgctggta gcaaatataa actatctgta
    68761 gtgtcacctg tagactcaaa aggattgttt acagaagaag cacctggatt tgaaggtatc
    68821 ttttatgaca aagcaaatcc tatgatcact gatctattaa aagaaaaagg agctttgctg
    68881 aagctagatt tctttactca tagttatcct cacgattggc ggacgaaaaa gcctgtgatc
    68941 tatcgggcaa ctccacaatg gtttgcatct atcgataaat tccgtcagaa tattttggat
    69001 gaagtagaaa aagtagagtg ggttatccca tggggtaaaa cacgtttgta taatatgatc
    69061 cgtgaccgcg gcgattgggt aatttcccgt cagcgtgcat ggggagtacc acttccgatt
    69121 ttctatgctg aaaatggaga agcaatcatc acaccagaaa caatcgaaca tgtggcaaaa
    69181 ttatttgctg aacatggatc aattatctgg tttgaaaaag aagcgaaaga acttcttcct
    69241 gaaggattta cccacccagg ttctcctaat ggcgaattta caaaagaaaa agatatcatg
    69301 gatgtctggt ttgattctgg ttcttctcat gaagctgttt tacgtcagcg accagaatta
    69361 tcattcccag cagatatgta tttagaagga tcagaccaat atcgtggctg gttcaactca
    69421 agtatcacga ctagtgtggc tatcaatggt gtagcaccat acaaatcagt tatttctcaa
    69481 ggatttgctt tagatggtga aggccgtaag atgagtaaat ctttaggaaa catcattgca
    69541 ccagaaaaag tgatcaaaca atttggtgca gatatccttc gcttatgggt atctagtgta
    69601 gattatgaag cagatgttcg tgtttctatg gatatcttaa gtcaagtggc agaagtatat
    69661 cgtaaaatca gaaatacgat gcgtttctta ttagcgaata cagaagattt cgatcctaaa
    69721 aaagatactg tttcgtacaa tgatttacgt tctgtagaca aatatatgac cgttcgattg
    69781 aaccaagtaa tcaaagaaat ccgagaagaa gggtacgaca aatataactt catgcatatt
    69841 taccgtacag ttatgaactt cttgacggtt gatctatcgt cattctacct ggattttgct
    69901 aaagatgtag tttatattga agcagaagat gattatcaac gtcgttgcat gcaaactgtt
    69961 ttctatcaaa cagctgtagc tttgacaaaa ttattgactc ctatcattcc gcatacagca
    70021 gaagaaatct ggtcattcct gaaagaagaa gaggagtacg ttcagttaag cgagtttcct
    70081 ggttatgaag agtttgcaaa ccaagaagaa ttgatggata cctggactgc tttcatggac
    70141 ttccgtgata atgtcttaaa agcattggaa gaagcaagaa acagcaaatt gattggtaaa
    70201 tctttggaag caaaactaac tgtttatcca aaacaacaag ttcgtgaaat gttaaaagct
    70261 ttggatgcag atattgcaca attattgatc gtttcaccag actactttga agttatggca
    70321 gcagatgaac aagtgccaga taacgctatg gtatttgatg atgttgctat tacagtggaa
    70381 aaagcagacg gggaaacttg cgatcgctgt cgccaagttc gtaaagatgt aggagtagat
    70441 gaaaaacttc ctcatctatg cggtcgttgt gcaaaaatcg ttgaagactt ctatcctgaa
    70501 gctgtagctg aaggattcga agaaaagtaa aagggaaaag ggggtgtgac atgttctttg
    70561 tcacaccccc ttttgttatc aatcgtttaa ttttccacgt tcacgatacc agttatctgg
    70621 ttgccagatc cattcgaatc cgtctttttc aagtagttcg aaagctgctt taggtcccat
    70681 tgatccagct ttgtagttgg gaaaatccgg ttgtgtttgg tcccaagctt ttcgaatgac
    70741 atccacgata tgccaagatt gagcgacttc ttcccaatga gagaagttcg ttccatcacc
    70801 attcaacgca tctaatagta atttttcgta agcttctggg ctattttcaa ccatttctgc
    70861 actatttcta aattccaatt tgattggttc cgtttcaaat ccttgcccta cttctttccc
    70921 attcaaggat aaagaaaatc cttcagttgg ctgaatatag atcgttagaa cattaggtgg
    70981 aagtgtcgta tcgtcacatg gttcatcgac agatgttttg aatacattaa caggaacttg
    71041 tttgaatacg atgttgatcc gagtcccttt ttcagtcaaa cgtttgcctg tacgtacata
    71101 gaaaggaaca cctgaccaac ggaagttatc gatagtgaat ttgcctgcga cgaatgtttc
    71161 agtctctgat tgagcatcca ctttgtcttc ttcgcgatag cccttgaatt gtacaccatc
    71221 caattcacct tgagcatatt gcccgcgaac gaagttttct aacacttctt cttcgttata
    71281 aatgcggata gctttcaatg ctttgatctt ttctgtacgg atttcttttt cggaaaatgc
    71341 tactggcggt tccattgcaa gtaatgctac aacctgtaaa atatgatttt gcaccatgtc
    71401 tttaagtgcg ccactatgat cgtagtagcc cccgcgatct tctacaccga ggttttcgcc
    71461 aaaagtaatt tggatattgt cgatatagcg attgttccat tgagcctcaa aaatattatt
    71521 ggcaaaacgg atagctgaga tgttttggat catttctttt cctaaataat gatcaatcct
    71581 gaaaatatct tgttctggga atacttctct gatttgctgg ttcaattcaa aagcagactg
    71641 gtaatcagaa ccgaacggtt tttctatgat caaacgattg aagccatttt ttgtcataat
    71701 attttgtgat ttcaaatgag aaacgatcgt tccaaaaaat tgaggtgcca tagccaaata
    71761 atagacacgg ttaccgttta attgatagtg tccatctaat cggtcagaca attcttttaa
    71821 tgtctgataa tgcgttgaat catttacatc atgggattga tagtaaaaat ggcttgcaaa
    71881 ggtagttgct tcttcatctg aaggattcaa tgcacggatt gtttctttga caacttcacg
    71941 gtagtgctca tcgctccagg gacggcgagc ggttccgatt acagcaaaat gctcgcctaa
    72001 atttcctttt ctgtaaaggc ggaacaaaga cgggtacaat ttacgctggg ctaggtcacc
    72061 tgtaccgccg aatatcgtaa acaatacatt tttttcatta gacatgtgga tacactcctt
    72121 tatattttat cgacaaaaat cgtgctggcc gccttaaagc tgacggggat tttctttcta
    72181 tcattttgga tagtgatcgg tccttcataa gctgcgatgt caacgatctc atattcttcg
    72241 ttgatattca ggtcaatagt gactaagtaa tccaacaatt ctttttcatc aagcactcgt
    72301 gcgatacgca cttttgtacc gacaggatag gactccaatg tttgtcggcg ttcttcatgg
    72361 acaacttcct cttttttagg aatcgatcct ccatgaggac aatgttcagg atgattcaaa
    72421 tagttttcta aatgatctgc aagatgttcc gaagtcacat gttccaatac ttcagcatca
    72481 tcatggactt cattccagct atagtctaaa tgttcaacta aaaaaacttc ccataagcga
    72541 tgtttgcgaa tcagactgct agctttttgc agtccgcttg cagtcaattg tacaccttga
    72601 taaggggaat gttctacaag ctcttctttc acaagtttag aaatcatttc actgacagat
    72661 gcagctgaga catctagact agatacgatt tgtttattgt tgactttcgt tgtatctcca
    72721 cctagttcta aaatgatttt caaataatct tctcgatttg gtgtcatatt cgatcatcct
    72781 ttcatggtaa aaaaccatca ttagtaagtg taacaaaaaa aacgttaatt gactattttt
    72841 ctaaataaaa aacaaaggat ttttagacgt ttttacaaca aaaaacgaaa gatgatagag
    72901 aagactcatt ctttcgttta taaaggatat atatatacat tgggagggat ctatttttca
    72961 gataacaact tatatgaagt gtctgcagat tcgtcataaa gagtttgttc gggataagaa
    73021 aagataagat aaaaaccata ctgatcacgt acgactaaac gatattcatc tatttctacc
    73081 acagatgtgc caagagactg accgttgatc aaaaacgtgt aatcagatgt aatctgtaat
    73141 tgttgttttt gctggctatc actaaagaac catgtacctt gatactttga gagtccttct
    73201 gacttttccg cttgtttttt ctttttcaag taaataaaac ttgagagaac ggtaatgagt
    73261 gaaccaagaa aatagatcat tttttttatc accaaatcac tcctttatga gaaaataatc
    73321 gcatagacat attctttctt ctttatttca ttgtaccgaa taaaagctaa aatttctcta
    73381 agactttcga ctggtttttt tactgttttt ctacaaatta tgataggata catagtagga
    73441 agaaaggtga cataatttga agtatcagca agtggaaata gcttttttta ttgaccgccc
    73501 gaatcggttt atagcacatt gtatgaaaaa agacggagaa ataattattg cgcatgtcaa
    73561 aaatacaggt agaggaaaag aggtcctgat accaggcgct gaagtggcta ttgtatttgc
    73621 tcctggacct aaacggaaaa ccgcctatga tctgattgct gtaaaaaaga agggtgactg
    73681 gtttaatatc gatagtcaat tgcctaatcg gctggcaatt gatggtattt tagacggcac
    73741 gatcctttta ccaggattaa ctggtccatt tacggtatac aagcgtgaag taacttatct
    73801 tcactccaaa tttgatattt atttagagac ggtggtcgga caaaaagcat ttgttgaagt
    73861 caaaggtatg acacttgaaa ataaagcgat tggtgctttt cctgatgcgc ccacactcag
    73921 aggattgaaa catataggag aattaacgta tgccgcacaa gatggttatg ccgcttatgt
    73981 cttgtttatt gctcagttcg aacatcttca tctggcaacg atccatgaag aaatgcaacc
    74041 agcactagct gatatggttc gacatgcgca gcaaagcgga gttcaaatcc tcgcctataa
    74101 ttgtcaagta tcggcagatg aggtaactgt caagcaggcc attccatttg atgtagatat
    74161 cccttttgaa gatcctatac aatagaaaga aggtaaaata atgatcaact taggtattat
    74221 cggaacaaat tggattactc atcaatttgt ccaagcagct ttaagcacaa aaaaatatga
    74281 attgacagcg gtatattcta gacatttgga aaaagcacag gaatttggtt ctcattatga
    74341 aggggatatc gaattcgcaa cagatttgaa gacttttttc aatattactc atatggacac
    74401 catttatatt gcttctccga atagtcttca ctttagtcaa gctaagcaag caattttggc
    74461 aggaaaaaat gtgatagttg aaaagccagc tttttcgacg ccaaaagaaa tggatgaaat
    74521 catccatttg gcaaatgaac acaatgtgta tttttttgaa gcagcaagaa atatccatga
    74581 atcaggtttt aaaaagattg ccgattactt accattaaaa gacgagataa tcggtgcaaa
    74641 tttcagctat atgaaatatt cttcgcgcta tgaccaagta ttagatggag aagaaccgaa
    74701 tatcttctcg actcatttct caggtggtgc attgatggat ttaggtgttt accttgtata
    74761 tgcagctgtt gcttggtttg gaatgccaaa agaagtccat tatttcccaa gaaagatttc
    74821 tactggtgta gatggtcttg gatgggggat cttacgttat gagaattttg atgtatcgat
    74881 ccagccaggt aaaatcggtg attcttattt accgtcagag atttattttg actcaggaac
    74941 gctagtgatg aatggagtca atgcaattga acaagcacaa tttcatgatc gcgcgcataa
    75001 agaaatcatt gatttggcgg taacagctgc agaaaatcca atggcagatg aagcagagga
    75061 ttttgccaat gtcatggctc atccgaaaga tccagcttgg ggattgttgt atgaagagtg
    75121 gatcgagcta gctagaaatg tgaaccaagt gatttatgat ttaagaaaaa atggcggcat
    75181 caaattcgat gcagataatg aggaagattt ttaatgaacg aatttgtaca agtatttccc
    75241 gaaaattggc agaaaaaata tgaggataaa gggtttatct caccttctct tattcaacga
    75301 gcagtttttc agccactaag taataaacaa agtatagtag cggtttctcc tacaggttca
    75361 ggtaaaacac tggcttatct ttggcctcta ctactaaacg tggaaccagg tgaagcaagt
    75421 gcgttggtca tttttgcttc ttctcaagaa ctagctattc aagtagcaga tgttgcacgt
    75481 gaatggggaa aagacaaaga gttgaaagtc caatctttgg tgggcggagc caacgtcaaa
    75541 cgtcagattg agggtttgaa ggaaaaacca gaaatcttga ttggtacacc aggaagaata
    75601 ttggaattga tgaaagcaaa aaaaataaaa gcacatcaag tgaaaacaat ggtatttgat
    75661 gaagcagacc agctatttga cgctgggaac agccaaatca ttgatcaaat tcttcatcaa
    75721 gctccaacag agtatcagct agcttttttt tcagcgacag cagatcgttc ccttgaaagc
    75781 attgaaaaaa tcactgggaa aacgtttgag acaattgacg tgacagcaga ggacgattct
    75841 cgaaaaggac tgagacatta ctttttacgt gtaccgatac gaaagaaaga tgactacttg
    75901 agacgtctca cgcatataga agattttcat ggtctgattt tctttaatca actgactgaa
    75961 ttaggcgtaa tggaagaaaa attgctgtat agaggtgtac cggtaggaag tctggcctct
    76021 gatcagaaca agctactccg aaaagcagcg ctagaacaat tcaaaaagaa aatggtcaga
    76081 gcacttttga ccacagatgt ggcggctaga ggtttagata ttacaggtct tccttatgta
    76141 gtgaatgcag aagttccgtt aagtgaagaa gcttatttgc atcgttcggg aagaactggt
    76201 cgaatgggta atgaaggaat agtaattaca ctcgtacaag ataataatct aagagattta
    76261 aaacggcttt tacgtccatc aaatataaca cttgaagaga tttttttata cggaggtaag
    76321 ctgcaaactg agcagccaac caaagaagat gcagctcctg cagtaaataa aaagtatgcg
    76381 aaaacttttt ctggaaagga gcagaaccca gaatataaca aagaaaaaat aagaaaaaaa
    76441 gttaaaaata aatcaaaaaa agagagaaat aaaggagcaa ggcgcaaata agcgcctttt
    76501 tatgctatga ttatgaaaac gggaacaaaa ttggttgcca gaggaggggt tatgtgaaag
    76561 aacgtcaaga agagatcttg cagctattga cagaaaacca acaaggattg acagcaagtg
    76621 atgtagctga gcgcttaact atcgatcgaa gcaatgcaag ccgatattta agtgaactat
    76681 acaaggccca tcatattgta aaaaccgctg gccgaccagt cgtttactct ttgccaacgg
    76741 aaaagtcaaa atctgatgaa gtgcatgtcg actcttctac gcaagtgact tttgaaacac
    76801 tcgttggaga aaatgattct ttaaaagtca gcatacagca ggcaaaagct gcgattcttt
    76861 atccaccgcg ggggttgcat acgattattt ttggagaaac tggtacaggg aagtcgatgt
    76921 ttgctgaatg tatgtatcat tttgcaatcg attcagagat gctttctgca gatgcgccat
    76981 ttgtttcttt caattgtgcg gattatgctc aaaatccgca actgctcttt ggacacatct
    77041 ttgggatcaa aaaaggggct tacacaggag cagctcaaga tagtcccggt ctaatagcca
    77101 aagcagacgg cggtatattg tttttagatg agatccaccg tttaccgccg gaagggcagg
    77161 aaatgctgtt tactttcatt gataaaggta tttaccgtcc gcttggagaa agtgcacaag
    77221 tacatgaagc atctgttcag atcattgggg cgacgactga atcttcagaa agtttcttat
    77281 ctacgttcaa tcgacggatt cctatggcaa tcaccttgcc gaatcttgct tcacgttcac
    77341 tggatgagcg ttatgaaatc atctctcttt ttatcaaaca agaagcgaat cgtttgaatc
    77401 aacgtatcga tatcgaaaaa gaagcgattc ttgccttcat gctttatgat gcggaaggaa
    77461 atatcggtca agtgaaacga gacttgaaac tagtctgtgc aaaatctttt ttacattacc
    77521 gtactcatca tgagaaaaag ctaatcattc gaaaagaaga actttccctt caagtacaaa
    77581 aaggattact gaaaatcaag gaaataccgg aacgtctcga ccgattcatg gacagcaaaa
    77641 gtcagtattt gacatttgaa ccaggatttg ctgatgttgt ctggtcacaa gatccagaac
    77701 gaaacatgca ggtctataac gatattgagg aaaaagtcac ttctctttcc gaaaccggtg
    77761 tggagaacat tgatctagag accctcattt caaaagatgt ggatgcttat ttccagacat
    77821 atgtcaaaga attgactaaa agttcgattc ccaaagatct tttgccagat gaaatctggc
    77881 ggttaaccaa tcaattgtat gatgtggctg aaaaacggtt agaccgtatt tataatgaaa
    77941 aagctcgttt tgcttttgct ttgcatctgc aaagtacatt ggatcgtgtg agagaaggtc
    78001 atatgatcgt ccacccagat ttgaataacg tacggaaaaa ttttaagagg gaattccagg
    78061 tagcaatcga tctttcaacg atcattgaag aagaagaaca agttgagatt ccttttgatg
    78121 aaatcggctt tattagtatg tttctttcta tcaatctagg agaaagtgag cctgttaaag
    78181 aaaacaaagt agatgttctc atattgatgc acggcagaca gactgcttca agcatgttgg
    78241 agatggcaca agaactatta ggaacaaaag tagggaaagc ttttaatatg ccactggtta
    78301 tggaagttca aacaatgtat gaagaagtgt tacaatatgt caaaaactat caagagcatt
    78361 tgacgaatgg cctactcttg ttaacagata tgggatcatt aaacaccttc agtgatttga
    78421 tttatgaaga gactggcatt cggacgaaag ctatcacgat gacaagtacc atgattgtga
    78481 tagaagcgat acggatggca agtgttggaa gaagcttaga agatatttat caaaatatcc
    78541 agctttcctt tgaatcgatc gtacgtgatc agttcaaagc ggaaaagtct gaaactttac
    78601 agcgtaaaaa agccgtaatc gtgacatgct ttactggtga aggagtagct gccaaattat
    78661 atcagcgtat tatgccagtg attaatcaaa ataaggtaga actgatccag atgcagtttt
    78721 tagaacgtga atcattcaaa aaacacatcg atgccttatt ggaagaatat gagatcaaag
    78781 caattgcggg aacagttgat gttgattatc aaaatatccc atttttttca gcctatgacg
    78841 tttttgatga tgaaaaacta aatattttaa agcgtatagc tgatgatgag atcccgacag
    78901 ctaaaattgt cgattcactt gctggatcat taagcgatgt cttttctatt gatgaactga
    78961 tccgttcatt gcaaaagaca gttcagcaga tccagaccga tttgtcgatt atcgtggaac
    79021 ctggagtaga tactggaatc gttatccatt tagccttcat gttagacgga ttgatcaaac
    79081 aagaaagcca tcggcaattt gagaaactgt ctattttctc aaaaatttat cgtttggaaa
    79141 tggatatcgt tcgggcaaac ttgatggcac tagaacgaaa atatcaggta aaaattccag
    79201 aagatgatat cgcatttctt acacaaatgt ttcttgaaaa taaaataaaa aaatgaacac
    79261 actaatcata ctgtttacac actaaacatc tgtgtgcgca ctataaaaaa gagctaaaaa
    79321 gcatgaaaac gctatttcag ctcttttttt tattggcatg cttcttgcgt tatataaaag
    79381 tgtaaaaaaa taaaaggagt gttaaaaatg gcaaaaaaaa caatcatgtt agtttgttca
    79441 gcagggatga gtacgagttt attggtcaca aaaatgcaaa aagcagcaga agaaaaaggg
    79501 atggaagccg atatttttgc ggtctctgcc tcagatgcag ataataattt agaatctaag
    79561 aatgtcgatg tattattatt aggtcctcaa gtacgtttca tgaaagctca atttgaacaa
    79621 aaacttgcgc ctaaaggtat tccattagat gtcatcaata tgcaagacta tggcatgatg
    79681 aacggagaaa aagttcttgc acaagcagaa aatctaatga aataaatcaa gtatgcagtt
    79741 gggggtataa caacgtggaa gatcaacaaa atctagaagc catcatgggg ttaatcatgt
    79801 ttggcggtaa tgcaaagagc gatgcaatgg aagcgattgc ggctgctaaa atcggtgatt
    79861 ttgagttagc ggataggaaa atcgctgatg cagaagaatc acttatacaa gcgcatcatg
    79921 cacaaacaga catgttgaca caagaagcac aaggaaatca tatgcaagtg acattattga
    79981 ctgttcacag tcaagatcac ttaatgacat ctattgcgtt tactgattta gcaaaagaaa
    80041 tcatcgaact ttatcgtcgc gtatacagcg aataaataca acttcttttt tgaacggaag
    80101 aagaaaaatt tttgaacgga ttttttattt tgttataagg agaactctct ttatataaaa
    80161 aacttatagg aggaagtaaa aaatgaacgg actatcaagt tggatggaga aatacatcct
    80221 gccggttgcc gcaaaaatcg gagcgcaaaa acatttagtt gctttgcgtg atgcttttat
    80281 cggcacactg cccgcaacaa tggcgggatc agtcgctgta atgatcaatg caattattcg
    80341 tgatttaccg caacagttta acagtaccta tgctgacagt ttagcagcag acaaaggaat
    80401 ctttgccgta ttgaatgtca tcatcggaat caatggtttt gtctggaacg gtacactagc
    80461 aatcgccgga ttgatctttg ccttttcttg ggggtataat cttgcccgag cttatggagt
    80521 aaatgatcta gctgggggga tcgttggttt agctaccttg attcaaggta tttcctttgg
    80581 tccagcatta tcagcagcat tagatgtgaa tgttccttca aatgtcgcag atgcaatcaa
    80641 tagttctgag attggtgcaa cgattgcaga tggcacactg tcagttggat tatggggttg
    80701 gctaaaacta gatcatttga atggaaacgc ttattttaca gttatgatca tgggagctat
    80761 ttcagtcatt attttttgta aattgatgct tgccaacatt acgatcaaaa tgcctgattc
    80821 agtaccacca gcagtatcta aagcatttgc agccatttta ccagcgacga tcgctttata
    80881 cgtcattgct attatcaatt atatcgtagg tcgtgtagct gatgggcaac tgatcattga
    80941 tttggtgcaa aaatatattg cagaaccatt cctaggatta tctcaagggt tgggagcagt
    81001 attgatcgtc acagtatttg ttcaagtatt ctggttcttt ggtattcatg gaccaaatgt
    81061 attggcacca gtgctagagg gaatctgggg acaagcacaa ctaatcaata ttgatatttt
    81121 ccaaaaaggt tataacggaa aaactggtac accagccgtg ttagatgcaa tcgaagatgg
    81181 aaaagcttat atgtgggtcc gcggatcgtt cgatgctttt gcatggtttg gcggatcagg
    81241 cggaacgatc gtcttactga tagctatctt gattttctct aaacgtgcag attatctaac
    81301 agtagctaaa ttatccattg ggcctggtat cttcaatatc aacgaaccag tgatgtttgg
    81361 tctgccaatc gtattgaatg caatcatgtt tatcccattc ttgattgctc cagtagtagc
    81421 aacagcaatt ggatggacag ctacttatct tggcctagtc gctccggtat cgcagcaagt
    81481 cacatgggta gtaccgccgt tcttattgtc attcttagca acaggagcgg attggcgggc
    81541 accgatcgta acattagtat gtatggtcgt gactttcttg atttgggcac catttgtcat
    81601 ctctgcaaac aaaatggacc ctgccgcagg cgaacaacaa taagatgaac gtgaggagcg
    81661 ggacgcttta agtttcgttc ctcataatga attttcgagg aggaactgaa atgattacaa
    81721 ttgccagtgc aatgcaacaa gatgaaatca aagaattgtt agaaggatac aatgaagaag
    81781 gaatgacttt tacatttaaa gaaaaacaag gaatcaagct gttctttgaa acaagtgcag
    81841 aagatccgga agttgcggct aaaaaagcaa aggaactaat caaagcacag ccttggggaa
    81901 cagtccttta ttttcaatca acagctgttt agaaaatggt ttaaaaagat aggatggttg
    81961 agatgataaa aaacggttta tttttatgta gtattggttc tttgatcgtg ttgtatagtt
    82021 tgaatccaca ttcgcaaagg tatgatttgt atagtatggt cacaggtctt tttctaacag
    82081 gattaggaat ctatttcttc atgaagggga aaaaaagaga agaagcaaag gagaaaaata
    82141 actaatggaa atcacgaaaa aattaaatat tccagcatcg tttttttacg ataaagttat
    82201 ggactctgtc cttttcgatg tacgtaaagc gaccggcaaa tcagtaacta gaaaacagct
    82261 gaaaaatttg gagtacgtca aacaattctc gcaaaataac cgggctcgca taaaaatcga
    82321 agaagtcatt gaaaaccagt cttataagtt tcgtacatcg acaacgaaga atgaattcgt
    82381 cgttcattat caaatcaaac cattggacga ttcgacctgt gaggttcatt acacagaaaa
    82441 aatggagtct tatggcatgc ttcaaaagtt gaatgatatg cttttaggaa caatcttgat
    82501 gtattttaaa cggagacgtt tcaaaaaaat gctagatatg atcgaagcaa cgtattaaat
    82561 caatgaaaca tggtactatt tagttattga aaaatgaagt aagctagaag gaatttttca
    82621 taggaagata gatctttcct gcgaatcctc actagctttt ttgtaggaag gggcagcaaa
    82681 atgacaaaca aagtaagaga gctaggtgta tcttcgattg aggaaatgtt tgatttagcc
    82741 acttatgcat ttaatggggc agatacacct gaaaggcgag aacgttttcg tacacttgcg
    82801 gagaattctt ggaattacgg attttttgat gaggaggatc ggctaacaag tcaagtcatg
    82861 gcgacccctt ttccagtgaa tttttacgga caagagtacc taatggccgg cattggctat
    82921 gttgcttctt atccagaagc aaggggacaa ggcgggatca atcagatcat ggaaaagatc
    82981 cttgaagatt gccgtgatcg taaagtaagc ttatcttatc tggcgccatt ctcctatccc
    83041 ttctatcggc gctatggcta tgaacaatca tttgataaaa tcagttatca actaaacagt
    83101 cgtgatgtgc cgtttatcaa aaaaaaatct ggaaaaataa agcgtatgga ttttgaagat
    83161 gcaaaagctg caatcagaag aatttatgaa cagatgccgg aaaaccaacg agctggtctg
    83221 aagcgcgaag actggtggta tacgtataaa ttcaagcaaa aaggcaatta ccagtttgct
    83281 ttgcattttg atgataatga ggaagcaacg ggatatatcg tttatcagct tgcaacgtct
    83341 tcctttatta ttgcggagtg gggatttttg gatcatgatg ctttttgcag ccttgttcgc
    83401 ttcgtaagtt ctcacaatgg tgcttttgaa acatttgaat attcatgcgg ctacggcggt
    83461 aatgatcaaa attatctatt agaaaatcca tttgcttctg taatgatcac accttatatg
    83521 atggcgagga tcgtagatat ttctttgttt ttggaaaatt atccatttaa aaagagacaa
    83581 gctgcatttg ccctacaaat agaagaagat cattatgcga aatggaatga aggaatcgtg
    83641 gaagtaacaa tcgaaaatgg aaaaaatcat ctctcaaaag tagaacaaac aaaactccca
    83701 atcgttcgag gaagtatcca acatatcacg cagctattat tagggtatcg taaagtggaa
    83761 gagttggtat tccaagaaag aatacaggtg acaaaagaat atgaagaaac gttaagctca
    83821 atcttgccac agcaacagcc aattcttaat gactattttt gataaaaaac gggtgatgac
    83881 aatatttttg tcaccaccct tttgtttttc atttggcatt tgaaggaaaa tgttaaagta
    83941 aatcctcttt tttcgtaaat ttgaattttt tgttttcttt tgttttaggg ttttcttttt
    84001 tgtggtcttg gattgcttct ttcgtaatct tttcccgatt ttttttagat gaatctaagc
    84061 gttctgcgta tactttttct tttggcttat cattcattgg aaaggacctc cctacattat
    84121 ttcattctac ctctagttta tcatctgtta aggactagtg gaaacgtttt gcctaactta
    84181 taagctgatt tttttagcag ataagcttgc aagtaaaaaa caaacaagct acaattgatt
    84241 tattgaaatt gttcctgtag ctcagtagga tagagcaatc gcctcctaag cgataggtcg
    84301 ctggttcaat tccagtcagg aacgtataaa taacccctgt atcctttgcg gtacaggggt
    84361 ttgttttgta tttgtgccaa aaatgtgcca aactagttat catttttata taaactatcg
    84421 aacacagtat tgattttttc tttatcctga tcttcaagct ctcgaacaat atgaa
//
LOCUS       DACEUZ010000002        57637 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.002, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000002 DACEUZ010000000
VERSION     DACEUZ010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 57637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57637
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.002"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(57..974)
                     /locus_tag="IXG44_000089"
     CDS             complement(57..974)
                     /locus_tag="IXG44_000089"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HAQ1656835.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            1176..1613
                     /gene="zapA"
                     /locus_tag="IXG44_000090"
     CDS             1176..1613
                     /gene="zapA"
                     /locus_tag="IXG44_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HAQ1656836.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            1671..2219
                     /locus_tag="IXG44_000091"
     CDS             1671..2219
                     /locus_tag="IXG44_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HAQ1656837.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            2334..4694
                     /locus_tag="IXG44_000092"
     CDS             2334..4694
                     /locus_tag="IXG44_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAQ1656838.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            4781..5095
                     /gene="trxA"
                     /locus_tag="IXG44_000093"
     CDS             4781..5095
                     /gene="trxA"
                     /locus_tag="IXG44_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HAQ1656839.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            5277..7055
                     /gene="uvrC"
                     /locus_tag="IXG44_000094"
     CDS             5277..7055
                     /gene="uvrC"
                     /locus_tag="IXG44_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HAQ1656840.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKKFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELANKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGIDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            7200..8099
                     /locus_tag="IXG44_000095"
     CDS             7200..8099
                     /locus_tag="IXG44_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1656841.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            8092..9354
                     /locus_tag="IXG44_000096"
     CDS             8092..9354
                     /locus_tag="IXG44_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1656842.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            9517..11415
                     /locus_tag="IXG44_000097"
     CDS             9517..11415
                     /locus_tag="IXG44_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HAQ1656843.1"
                     /translation="MDRYLDLLSEKFPTTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETEISQFTIFTAYPEYVLTTDWYKKQDKS
                     QLIHRLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFTP
                     KNRKDLSEREQEELEKIHQALSILQFKSEHQLLNRRPEFKMDDRKLLEKINYEESTIV
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTANEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDAKEEGSAIINGHTPVKVKKGESPIKANGKAFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRNTTKGMELQQQITQLQR
                     LLDYRKDD"
     gene            complement(11714..12433)
                     /locus_tag="IXG44_000098"
     CDS             complement(11714..12433)
                     /locus_tag="IXG44_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HAQ1656844.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIEKIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFATHEVKIVSD
                     SLLSDLLPDSYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDNMHKILGVQWHP
                     ELSHRVDSSEQRLFDFFVNEF"
     gene            12770..13102
                     /locus_tag="IXG44_000099"
     CDS             12770..13102
                     /locus_tag="IXG44_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1656845.1"
                     /translation="MKQVLEALEQIRKAEEENEKRARTLRQQLKTYAEEKEQELEIIQ
                     TNSRQQLKQLVEEKEQFEKQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            13089..15092
                     /locus_tag="IXG44_000100"
     CDS             13089..15092
                     /locus_tag="IXG44_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HAQ1656846.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKHQELSLQDL
                     EANYSEENFMNKLEEVLDLKEKWEQLTEKRDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAAIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKVKERFIHATYLIVLQGWVDAEEKQNLMHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLIEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLFVLPRGMKRFVKFFEILSVPSIIWGLIYSSFFGQALPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALIGVMLIKNTAFVYLGAGLSIIAALCILIVPIIQSPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            15111..15581
                     /locus_tag="IXG44_000101"
     CDS             15111..15581
                     /locus_tag="IXG44_000101"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HAQ1656847.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            15602..16186
                     /locus_tag="IXG44_000102"
     CDS             15602..16186
                     /locus_tag="IXG44_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HAQ1656848.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQIETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAIAEMENWDESEQ
                     IQFIKNSLYSLPLTGKVTFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            16201..17187
                     /locus_tag="IXG44_000103"
     CDS             16201..17187
                     /locus_tag="IXG44_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HAQ1656849.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNLDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFEPETIRKRVRQVYDA"
     gene            17177..17488
                     /locus_tag="IXG44_000104"
     CDS             17177..17488
                     /locus_tag="IXG44_000104"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HAQ1656850.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            17510..19291
                     /locus_tag="IXG44_000105"
     CDS             17510..19291
                     /locus_tag="IXG44_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HAQ1656851.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            19284..20660
                     /locus_tag="IXG44_000106"
     CDS             19284..20660
                     /locus_tag="IXG44_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HAQ1656852.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            20667..21302
                     /locus_tag="IXG44_000107"
     CDS             20667..21302
                     /locus_tag="IXG44_000107"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HAQ1656853.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            21332..22687
                     /locus_tag="IXG44_000108"
     CDS             21332..22687
                     /locus_tag="IXG44_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HAQ1656854.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(22879..24273)
                     /locus_tag="IXG44_000109"
     CDS             complement(22879..24273)
                     /locus_tag="IXG44_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAQ1656855.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFNSPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            24365..25336
                     /locus_tag="IXG44_000110"
     CDS             24365..25336
                     /locus_tag="IXG44_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HAQ1656856.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            25333..26115
                     /locus_tag="IXG44_000111"
     CDS             25333..26115
                     /locus_tag="IXG44_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HAQ1656857.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(26284..26655)
                     /locus_tag="IXG44_000112"
     CDS             complement(26284..26655)
                     /locus_tag="IXG44_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAQ1656858.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            26789..27232
                     /locus_tag="IXG44_000113"
     CDS             26789..27232
                     /locus_tag="IXG44_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAQ1656859.1"
                     /translation="MDKPISGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(27275..28084)
                     /gene="yidA"
                     /locus_tag="IXG44_000114"
     CDS             complement(27275..28084)
                     /gene="yidA"
                     /locus_tag="IXG44_000114"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HAQ1656860.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(28103..29470)
                     /locus_tag="IXG44_000115"
     CDS             complement(28103..29470)
                     /locus_tag="IXG44_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ1656861.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(29454..30284)
                     /locus_tag="IXG44_000116"
     CDS             complement(29454..30284)
                     /locus_tag="IXG44_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HAQ1656862.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            30447..30884
                     /locus_tag="IXG44_000117"
     CDS             30447..30884
                     /locus_tag="IXG44_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HAQ1656863.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTGGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            30960..31580
                     /locus_tag="IXG44_000118"
     CDS             30960..31580
                     /locus_tag="IXG44_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HAQ1656864.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            31769..32275
                     /locus_tag="IXG44_000119"
     CDS             31769..32275
                     /locus_tag="IXG44_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HAQ1656865.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            32292..33191
                     /locus_tag="IXG44_000120"
     CDS             32292..33191
                     /locus_tag="IXG44_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HAQ1656866.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            33278..34162
                     /locus_tag="IXG44_000121"
     CDS             33278..34162
                     /locus_tag="IXG44_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAQ1656867.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(34354..34425)
                     /locus_tag="IXG44_000122"
     tRNA            complement(34354..34425)
                     /locus_tag="IXG44_000122"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(34390..34392),aa:Arg,seq:ccg)
     gene            34485..35819
                     /gene="rpoN"
                     /locus_tag="IXG44_000123"
     CDS             34485..35819
                     /gene="rpoN"
                     /locus_tag="IXG44_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HAQ1656868.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            36022..37059
                     /locus_tag="IXG44_000124"
     CDS             36022..37059
                     /locus_tag="IXG44_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SorC family transcriptional regulator"
                     /protein_id="HAQ1656869.1"
                     /translation="MLDEIKMIESVAPDIIEVLQERYKILRNIYWMQPIGRRSLSESM
                     GITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSLENFMDQLLGTHQTEQQLA
                     EYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEGENIIAVMGGTTMAVVAEQ
                     LSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKANGNHRALYVPEQLSLATYN
                     SLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEKELVMLKQANAVAESFGYF
                     FNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAIRAYMKNAPKQTWLITDEA
                     AANEILKGVTL"
     gene            37100..38101
                     /gene="gap"
                     /locus_tag="IXG44_000125"
     CDS             37100..38101
                     /gene="gap"
                     /locus_tag="IXG44_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAQ1656870.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            38211..39401
                     /locus_tag="IXG44_000126"
     CDS             38211..39401
                     /locus_tag="IXG44_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HAQ1656871.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            39505..40260
                     /locus_tag="IXG44_000127"
     CDS             39505..40260
                     /locus_tag="IXG44_000127"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HAQ1656872.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            40376..41674
                     /gene="eno"
                     /locus_tag="IXG44_000128"
     CDS             40376..41674
                     /gene="eno"
                     /locus_tag="IXG44_000128"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HAQ1656873.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            42074..43213
                     /locus_tag="IXG44_000129"
     CDS             42074..43213
                     /locus_tag="IXG44_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HAQ1656874.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(43280..43855)
                     /locus_tag="IXG44_000130"
     CDS             complement(43280..43855)
                     /locus_tag="IXG44_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ1656875.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(43860..44834)
                     /locus_tag="IXG44_000131"
     CDS             complement(43860..44834)
                     /locus_tag="IXG44_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HAQ1656876.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIAFALKVNPNIGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEGAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(44858..44902)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            45117..46085
                     /locus_tag="IXG44_000132"
     CDS             45117..46085
                     /locus_tag="IXG44_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ1656877.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMN"
     gene            46198..46476
                     /locus_tag="IXG44_000133"
     CDS             46198..46476
                     /locus_tag="IXG44_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAQ1656878.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            46594..46830
                     /gene="secG"
                     /locus_tag="IXG44_000134"
     CDS             46594..46830
                     /gene="secG"
                     /locus_tag="IXG44_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HAQ1656879.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            46942..47688
                     /locus_tag="IXG44_000135"
     CDS             46942..47688
                     /locus_tag="IXG44_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ1656880.1"
                     /translation="MRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKADYTV
                     YAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFAVRA
                     LEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSLAEQ
                     QLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLRWYPE
                     SGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            47691..50048
                     /gene="rnr"
                     /locus_tag="IXG44_000136"
     CDS             47691..50048
                     /gene="rnr"
                     /locus_tag="IXG44_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HAQ1656881.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWATELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKTRNGKSGKRE
                     GKQAGKRPERNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            50070..50534
                     /gene="smpB"
                     /locus_tag="IXG44_000137"
     CDS             50070..50534
                     /gene="smpB"
                     /locus_tag="IXG44_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HAQ1656882.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIIRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            50836..51555
                     /gene="atpB"
                     /locus_tag="IXG44_000138"
     CDS             50836..51555
                     /gene="atpB"
                     /locus_tag="IXG44_000138"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HAQ1656883.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMVISLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            51608..51823
                     /gene="atpE"
                     /locus_tag="IXG44_000139"
     CDS             51608..51823
                     /gene="atpE"
                     /locus_tag="IXG44_000139"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HAQ1656884.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVVALILVFGV"
     gene            51914..52438
                     /gene="atpF"
                     /locus_tag="IXG44_000140"
     CDS             51914..52438
                     /gene="atpF"
                     /locus_tag="IXG44_000140"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HAQ1656885.1"
                     /translation="MLNQLAIAEVGNAMLGNVIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            52425..52967
                     /locus_tag="IXG44_000141"
     CDS             52425..52967
                     /locus_tag="IXG44_000141"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HAQ1656886.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNKAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYAYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMNYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLENMRNQLNR"
     gene            52990..54546
                     /locus_tag="IXG44_000142"
     CDS             52990..54546
                     /locus_tag="IXG44_000142"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HAQ1656887.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            54560..55462
                     /locus_tag="IXG44_000143"
     CDS             54560..55462
                     /locus_tag="IXG44_000143"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HAQ1656888.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            55485..56891
                     /gene="atpD"
                     /locus_tag="IXG44_000144"
     CDS             55485..56891
                     /gene="atpD"
                     /locus_tag="IXG44_000144"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HAQ1656889.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            56905..57327
                     /locus_tag="IXG44_000145"
     CDS             56905..57327
                     /locus_tag="IXG44_000145"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HAQ1656890.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
BASE COUNT        19081 a        10148 c        12336 g        16072 t
ORIGIN      
        1 gagaacaccg tttattcgga acaagggact gtgacatgac ttttgtcaca gttccattat
       61 ttttttagca gcttcaaagc tttttctgta ttggcaaaat gaagtttggc atatttttca
      121 agcataggca tgccgccttt ctccaatagt tttgccgcta cttggtcaga tttcgttcca
      181 gctcctgatg gaagggtgat tccagcttca gctgatgctt tgtgtaactc ttccaaaaag
      241 ctggcacgac tgatgataga agcagcagca acagctaaat gatattgttc tcctttagta
      301 acgaagtaca gtttttcttg taattgattt ttttctaaac gcgcatattt tttaaaattt
      361 gcttccggcg taaactgatc gatcaaaatc gcttctggtt tttctggctc gatttttttt
      421 agcagcaaat ggatcgcttg attgtgcaaa gcgactttca tccgaacggc attgtatttt
      481 ggttggatct cattgtattt ttctggagat aagacaagca gtttataggg gatggttttt
      541 tttaagatgt cagcaagctg gatgatttgg ctatctttta actcttttga atctctaaca
      601 ccaagtttac gtaattctgc aagctgatca cgtttcacat aagcagcaca tactgttagc
      661 ggaccaaaat agctgccatt tccgacctca tcactaccta aaacggataa ttgactgata
      721 ttttttggca aagaagaggc tgtttttgct ggcttggctt gtttatcctg tgcctccccc
      781 catttatcag cttctattgc tgcattcgta ccttgaaaca tcacttttcc ggatgtataa
      841 gcggtaatgg tcgtcccgtc tttttttgca gcaaagctag tataaggaat ctgtttattt
      901 aacaaataag agcgatagta tgctttcatt tgactaagtt ggtcgtttgt tgctttgata
      961 atgacgttgc tcataaaatt ctcctcgttt ttatacataa aactataccg caactcacga
     1021 cttttcgttt tctaagagat tgcgagacat agcatttttt taaagtttat catataatag
     1081 ttaagtatgt gaaaaatatt tgtggagaac ggtgaaagaa ccaacttcat ggtacactaa
     1141 ataaaacttt catcaaatag gaggaaatga ttcgtatggc gcacgaaaaa acacgctaca
     1201 aagcagtgat tgccaaccaa acatatacta ttatcggacg ggaaaccaag catcatatgg
     1261 atatcgtaac gaagctgatc aatgaacagt tagcagaatt gaaacaatta tcaccacaaa
     1321 tggataatga acaagcagca atcttgatgg ctgtcaatgc gctttcggat caattgaaga
     1381 aacaagaaag aatcttggag ctggaagaag aaacagcaga attaaagaaa aaaatgatca
     1441 aatttacaga gcttgaaaat cgtgtgaaac gcattgaagc aatcgagaac gaagctcggg
     1501 aagtattgaa agaaaatggt caagcagatc ataccattaa aaatcatgtg gaagcacaac
     1561 agattttaaa cgagaaacga aaaggacaaa ttaaacaaaa atcttctaat taagatcctt
     1621 ttatcaaaat aagaaaaatt taaaaaagtt agaagagaaa ggaacgtagc atgctgagtt
     1681 tgctgatttt atttttattg ctaattgcgt ttttttctgg agcacgtcgc gggtttgctt
     1741 tgcaaatcat attcgcgatt ggatatgtat tgtcatttat tgctgcccag catttttaca
     1801 aaccattagc aagtcatttg gaattataca ttccataccc agctgtcacc ccaacatcaa
     1861 agttagcatt ttttgatcag gtatttgctt ttcacctaga tgaagctttt tatgcaggaa
     1921 ctgcttttct gattattttg ctaattggct ggctgcttac ccgatttgtg ggggtttttg
     1981 tccatggatt gacgtatgtg ccgatcttga gacaggtaga ctggattgca ggaggcatcc
     2041 tgtcactaat catggcttac gttactatat ttttgatttt gcagttactt gcctttgttc
     2101 cattagatgt ggtccaaaat caattcggcg agcatagttt cgctcgtttt atcgttgaga
     2161 gaacgccatt tcttaccaac aagatgtatg agctttgggt aacgaatatt ttgaattaag
     2221 gaaaatagtg ctggaaaata tttcgttttt tgtacagagt agtttattta agaaggctgc
     2281 gtagcgtatg tccggcacag ctcttttttc atagtgaaag ataaggtgat aaaatgaata
     2341 accgaatcct tgaaacattg gaatttgaaa aagtcaaaca gatggtcaga caatttgtag
     2401 tgactgcaca aggaaaagaa gaactggcag aattggtacc agtgagtgag aaacaaacga
     2461 tcacaaactg gcttcaagaa actgaggatg gattgaaagt gcaacgtctg cgaggcggga
     2521 tccctatacc taaacttgaa aatatccgtc cccatatgaa acggatcgag atcggagcag
     2581 acttgaatgg agtggagctt gcacaagttt ccagagtatt gtctacgaca agcgaattga
     2641 atcgtttcat tgatgatctt tctgacagtg aaatcgaatt tgcacgtttg tatatgtggg
     2701 cagatcagtt agtcacgatc cctgttttga gccgccgttt aaaggaagca atcgatgaag
     2761 acggacgcgt gactgatgac gcttctcctg agttaaagag cataagacag aacattcggc
     2821 gaagtgaaca agctgttcgt gaacagttag atgggatcgt ccgtgggaaa aatgcaaagt
     2881 atttaagtga tgcgatcatc accatgcgaa atgaccgcta cgttatccca gtcaaacaag
     2941 aataccgcgg tgtatttggc ggtgtagtcc acgatcaaag tgcttcagga cagactttat
     3001 ttattgaacc aaaacaagtg gtggatttga ataatcgttt gcgtcaatac caaatcgctg
     3061 aacgaaatga gattcaacgt attttaagtg aactttctgc tgaacttgtt ccccatcgtc
     3121 aagaaatcat ccacaatgct tatgtgattg gtaaaatgga tctgatgaat gcaaaagcac
     3181 gatttggtaa agaagtaaaa gccatcgtgc cgggaatcag tgaagacaat catgtcgtct
     3241 tgaaacaagc aagacatcca ctgatcgatc aggaaaaagt agtttctaat gacatcacaa
     3301 tcggaaaaga ctatcaagcc atcgtcatta caggacctaa cacaggtggg aaaacgatca
     3361 cgttgaagac tttaggtctg ttacaactga tggggcaagc aggattaccg attcctgcag
     3421 gtgaagaaag tcaaatcggt atttttgagg aagtatttgc ggatatagga gacgaacagt
     3481 cgattgagca aagtctatcg accttttctt ctcatatgac gaacactgta gatattttat
     3541 caaaagtcaa tgaaaaaagt ctggtattat ttgacgagct cggtgcagga acagatccgc
     3601 aagaaggtgc tgctctagca atcgcaatct tagatgatct tggtaaaaag tctgcttatg
     3661 tgatggcaac aacgcattat cctgaattga aagtttatgg atacaatcgg gcaaatacga
     3721 ttaatgccag catggagttc gatgtggata cactaagtcc aacgtatcgt ttgctgattg
     3781 gcgttcctgg acgaagcaat gcgtttgaaa tctctagccg attaggtctt gatacagaag
     3841 ttattgatga agcgaagcag ttgatgaatg atgagagcca agatttaaac gaaatgatca
     3901 cagatttaga gaatcgccgg aaaatggcag agaccgaata tctggaaatg cgtcattttg
     3961 tttctgaggc acaagaactt cacgatgatt tgaaagaagc ttacagctat ttctttgaag
     4021 aacgtgaaaa agaaatggaa aaagcaaaga aaaaagcaaa cgaagtcgtg tcagaggcgg
     4081 aagaaaaagc agaaaaaatc attgctgata ttcgtaaaat gcaacagcag attgggcaag
     4141 gaaatgtgaa agagcatcag ttgatcgatg caaagaccca attagcaaat ctacatcaag
     4201 aagaaacttt gaagaaaaat aaagtattga agaaggcgaa agaacaaaaa acgttgaaac
     4261 cgggagacga agtgttggtt acgacttacg gccaaagagg gacgctttta cgtaaaaacg
     4321 ggaatcagtg gcaagtagaa atcggtatat taaaaatgaa tgtgtccgaa gacgagctga
     4381 cgcctgttgc tcctcaaaaa gagccgacac aacgagttat ccatgctgta cgatctgaat
     4441 ctagtagtca cgtgccgaat caattggacc ttcgaggaaa acgatacgaa gaagcgctaa
     4501 gtgaggtaga tcaatatttg gattctgcca ttctagctgg ttatcctcaa gtgactattg
     4561 tccacggaaa aggaacaggc gcacttcgca agggaattac tgattatctt aaaaaccatc
     4621 ggagcgtcaa aagctttgag tttgcaccgg caaaccaagg cggaaatggt gcgacaattg
     4681 ttaaatttaa gtaaagctta ctaaaggtaa gcagatggtt cgctatccaa gtatagaaat
     4741 gctatactaa tcatagattt taaaaatgga ggtctttgat atgtcacaag taattacaga
     4801 taagacattt aatgaagaaa cagataaagg acttgtattg attgattttt gggcaacttg
     4861 gtgtggtcca tgtcgtatgc aagcaccgat tttggatcag ttggaacaag aatatgatga
     4921 agaagaattc agaattgcta aaatggatgt ggatgaaaat ccagaaactc ctcaacaatt
     4981 cggtatcatg agcattccaa cattaatgtt gaaaaaagat ggtcaagtag tagaaaaagc
     5041 tgttggtgtt cattcaaaag aacaactacg tcaaatgatc gatcaatatt tataaaaact
     5101 tacagcaaaa ataagctgag ggtgggactg aatccagttc cgtcctcggt ttttttgcgg
     5161 ttgagttaag gttattcaaa ttaatatttt ttgagtataa ttacgttgtc aggctggatt
     5221 gtttttttga cagacatgct ataataactt taattgaata tgaaggggag taccagatga
     5281 acgaacgaat caaaaacaaa ttagctttac ttcctgatca gcccggctgt tacttgatga
     5341 aagataaaaa tggaacgatc atttatgtcg gcaaagccaa aatattaaaa aatcgtgtcc
     5401 gctcatattt tactggcagc cataatacga aaacggaacg gcttgttagt gaaatcgaag
     5461 actttgaata catcgttaca gaatcgaata tcgaagcatt gctcttagag atcaatctga
     5521 taaagaaaaa tgatcctaaa tacaacatta tgttaaagga tgataaaacc tatcctttcc
     5581 ttaagataac gaatgaaaaa tatccccgtt tggtgattac tcgtaaagta ctaaaagaca
     5641 aagcactgta ttttggacca tatcccgatg tcggtgcggc gaatgagaca aagaagatcc
     5701 tcgatcgatt atttcctctg agaaaatgca aacctagtca gacgaaggag ccttgtcttt
     5761 actatcattt gggacaatgt ctgtgtcctt attatttcga cgtagatccg aaagtctata
     5821 cgaaaatcgt tgatgaagtg aaaaagtttt taaacggagg acacgaaaaa atcgagtcag
     5881 agatccatca gaaaatggaa aaagctgctg aaaacatgga gtttgaaaaa gctgcagaat
     5941 accgagacca gatccgtgcc attgagacaa tcatgactcg ccaaaaaatg acaaatactg
     6001 atttactgga tcgagatgta tttggttatg cgattgacaa aggatggatg tgtgtccaag
     6061 tgtttttcgt aagacaagga aagctaattg aacgagatgt ttcaatgttt cctttctata
     6121 acgaagcaga agaagatttc ttgacttacc tcggtcagtt ttatcaggaa aatgagcatt
     6181 tcattcctaa agaagtcttg attcctgata cgatcgacaa gcccagtgta gaagctttgt
     6241 tagctacgaa agtgctgcaa ccaaaacgag gagaaaagaa aaaattagtt gaattggcga
     6301 ataagaatgc ctcagttgct ttgaatgaac ggtttgatct gattgccaga aaacaggaac
     6361 ggacgatcgg agcagttgaa cgtttaggaa acgcgatgaa tatcccgacc ccaatacgta
     6421 tcgagtcttt tgataactct aatatcatgg ggacgaatcc tgtggctgcg atggtcgtat
     6481 tcattgatgg aaaaccggca agaaaagaat atcggaaata taaaataaaa acagtcaacg
     6541 gaccggatga ttatgcatca atgagagaag tcatctatcg cagatattca cgagtgattc
     6601 gcgaagggtt gcctttacct gatttgatct tgatcgatgg aggaaaagga caagtagatg
     6661 tggcaaaaga tgtcttagaa aatcaattag gaatcgatat tcctattgct ggcatggcaa
     6721 aaaacgataa acataaaaca aatgagttgc tattcggtat agatttggct gtagtaccgc
     6781 ttgagcgaaa ttcaccagag tttttcttac tccagcgaat ccaagatgaa gtccatcgat
     6841 tcgcaatcac tttccatcgg cagctacgca gtaaaaacag ttttgcttcc cgattagatg
     6901 aaattgaagg gttaggacca aaacgaaaaa aaatgctgct taaagagttc aaatcgttga
     6961 aaaatatcac agcagccacg acagaagaat tacaagaaat cggactgccg aaaaatgtcg
     7021 cacaaaacgt atttgataaa ttacatcagg aatgaggaaa gggcacttta caaagaaagt
     7081 aaagtgtcct atcattttat gtacaacaaa aagagcgttt acgttttgtt ggtaaataat
     7141 aaatcgctta cgtctggtgt ctaaggatat atgtgatttc ttgaagaagg agtgaagaaa
     7201 tgttagaagt caaggatctt gttaaaactt ttgggaacta cacagctgtc gatcatgtct
     7261 ctttccaaat ccccgatgga aaaatcatgg ggctaattgg ccaaaacggg gctgggaaga
     7321 caacgacttt tcgtttgatc ttggattttt tgacacaaga ccaaggagaa gtgttatgga
     7381 atggccatca tcttggggag aaagattatg atgtcatcgg ttatcttccg gaggaacggg
     7441 gactttatcc gaaagtaacg attcaagacc aactgcttta ttttgcagaa cttagaggga
     7501 aatcaagaaa agagattgaa ccaaaaatcg atttttggat ggaaaaattt caggtaaaag
     7561 ggaaaaagac ggataaagta aaatctttgt ctaaaggaaa tcagcaaaaa gtccagctga
     7621 tcgccacgtt aatccatgaa cctaaattga tcattttgga tgagccattc agcgggttag
     7681 atccggtaaa tgctgaattg ctaaaagatg gaatattgga attgaaaaaa catggttcat
     7741 gtgtcatttt ttctagccac aatatggata atgtagaaaa aatctgcgat catttgatca
     7801 tgttgcgaaa cggaaaaatg gttttgaatg gaaaagtaca tgagattcgt gaatcatttg
     7861 gccgtacgaa gttattcttg gaatctggtt taagccaaca ggaagtagaa gaaatcgaag
     7921 gcgtgaaaaa agtgatcctt catgaagaca atacgttaga gattacctta gaagatcctg
     7981 aagctgggaa agaaatcttt actcgtgcga ctcagttcgg ctacattcct atgtttaacc
     8041 agcaaccgcc aactttggaa gagattttca aattgaaagc aggtgaaatc gatgcgtaag
     8101 ttttggatta ttaccaaaga tgtctattta aaaaatgtta agtcgatttc tttcttgatc
     8161 atgatattag tcccttttat catgctgggt atcatttatg tagcaggaaa ctttgctcaa
     8221 caaaatagtg atgtcaataa aatcggcgta ttggctgata atgagcaaat cagtcagcaa
     8281 atgagccaag tcaaaacaga tgattttgag ttcaaagcag ttgattctga agaaaaagca
     8341 caaaaagagc tttcggaaga aaaaatcgat gcgtatttag tagtctcaac tgagaatgat
     8401 aagataaatg ggatgcttta tagcgaaaat tcattaggac aaagtacaca actactgata
     8461 caacaacaac tgactggctt gcaatcgatg cttcgagcaa gtcaattagg catttcacca
     8521 gaagaagtag ctagcttgag ccaacaagca agtttgactc gacaaaaagt caattttgat
     8581 tcaaaaggaa aaatgacagt tggagaagat aactcgacag tccaatatgt cgtaagttat
     8641 gtagcaacga ttgtcttgtt cattattatt ttgacgtatg cgcagatcat tgcacaagag
     8701 atcgcttctg aaaaaggcac gcggatcatg gaagtgatcc tgtccagtac gactgcacaa
     8761 aagcattttt acggaaaact aacaggtgtt ttacttgttg cattaacgca aatggcatta
     8821 tacggtatta tttttggtat aggatacagt caattcaaag atatggaaat ggtcaaaaat
     8881 atattagacg gtatctcatt ggataacttg attggaccat tcttgtggta ttctcttcta
     8941 ttcatgatct ttggcatttt gattttttct gttttagcag ctttatgtgg ctctttggtc
     9001 aacaaagcag aagatacagc aaaagcaatt ttgccagtga cttatctatc tctaggcgga
     9061 tatatgcttg gattgatact aggagcttca gatccaaaca atatcgttat tcggatcact
     9121 tcgtatattc cattcttatc ttcatatatt atgccggttc gcttagcaaa tgagacagtc
     9181 ggtatgggtg gagcattgat ttccttgttg gtacttgtag ccgtaacatt cttattgatg
     9241 atcttgtctg caaacatgta caaatcgaat gtactcgtgt atagcgaagg cggattatgg
     9301 agttcattga aacaatcgat ttcaattatg agaaatgaac gaaaaaaagc ataaaatata
     9361 gaaaaatata gaaggaagct ggacatttat tgttcccagc ttcttttttg tatagaccta
     9421 tttctatttt tttattatat tggatatata tattcattga aaatagagca taacaacttt
     9481 ataatgttga gtataccaat ttttggaggc gttttaatgg accgctattt agatttactt
     9541 tcagagaagt tccctacaac ggaagatgtc atcacggaaa tcatcaatct cgaagcaatt
     9601 ttgcagcttc caaaaggaac agagttattt ttgagcgata tacatggcga atttcctgct
     9661 tttgatcata ttctgcgtat cggttcagga aatcttaaag aaaagatcaa agaccttttc
     9721 tctgaacaaa tgacggaaac ggagatcagc caattcacta tttttacggc ttatccagaa
     9781 tatgtgttga cgactgattg gtacaaaaaa caagataaat cccagctgat ccatcgtttg
     9841 atcgatcttt tacggtttac gacagtcaaa tacactcgat caaaagttcg aaaaggattg
     9901 ccgaaagaat atagctatat cattgaggaa ctcttgtata tcgatgatcg tatcaacggg
     9961 aaaaaagact atgtaaaaaa aatcattgaa caacttgtct tgatgaagga agaagaaaaa
    10021 tttttaacaa aacttgcgca gacgatccaa aaatctgtca tcgatcattt gcatatcgtg
    10081 ggtgatattt ttgatcgagg aactcaagca gccaaagtaa tggatcgaat catggatttt
    10141 tcctctgtag atatccagtg ggggaatcat gatattttgt ggattggtgc gtattgcggc
    10201 tctgaggcat gtttattgac gcttttacgg attgccgcac gctacaacta tttatttgaa
    10261 ttagagaaag aatatggatt aaatttacgt ccattatttt cttttgccca tcaaacctat
    10321 cagaagaatc cagtatttac ccctaaaaac aggaaagatc tatctgaaag agagcaagaa
    10381 gaattagaaa agatccatca agcactttct atcctccaat tcaaatcaga acatcagttg
    10441 ttgaatcgac gacctgaatt taaaatggac gatcgtaaat tgttggaaaa aatcaactat
    10501 gaggaatcaa cgatcgtttt agaaggtcag ctctatgagt tgaaagggac ttgttttcaa
    10561 acgatccagc cagatgatcc taaaaaattc ttggaagagg aacaaaaagt gatcgatagt
    10621 ttgatgtatt cattccagca ctcgttaaga atgcaaaaac acatgacgtt tttgatcgaa
    10681 aaagggggaa tgtatctcac gaacaatcag catgtactgt tccatggctg tatacctgta
    10741 gatgaaaagg ggcagctctt agcatttgaa ttagaccaat cttatcgcgg aaaagaactg
    10801 ctgggatttt ttgaaaaaca gataactgaa agcgcaaaag atttgactgc aaatgaagat
    10861 ttggcgaccg atttgatttg gtatgcgtgg agcgggccat tttcgccttt attcggcaag
    10921 agtaaaatgg caacttttga gcgctatttt ctcacagagc ctcatacgca cgtcgaaaaa
    10981 agcaatccct attatcactt gcgagatgaa gagtggttca gtgaaaaaat tttggcagaa
    11041 ttcgatgcaa aagaagaagg ttcagcaatc atcaatggac acacgccagt aaaagtaaaa
    11101 aaaggagagt caccgatcaa agcaaacgga aaagcatttg ttattgacgg aggtctatcc
    11161 aaagcttatc aaaaaacaac cgggattgct ggctactcat tactatgcaa ttcttacggt
    11221 ttccaaatcg tgacacatta tccgttcttg cctattgaac aacaatttgc aaaaaaatct
    11281 gatcaaacga atgtaaaaaa agtgatcgag cagcagctcc cacgcaaact taatcggaat
    11341 acgacaaaag gaatggaatt acagcaacag attactcaac tccaaaggct gttggattat
    11401 cgaaaggacg attgagaaaa atgctagcca acattgctca atagactaaa aagcgactgc
    11461 aacaaaaggc gtgtcgcagt ctccttcgga attagctcaa aaaacggtaa aatattagaa
    11521 gctatttttc tgtttatttc tctaatactc aggtcagggc gctgatttca cgacctcttc
    11581 cttaaacggt gttctcctgc caactcctcg gtcacaagtc acaatatcca acaaacatga
    11641 gaagctgttt tttgaaattg ctccttgtga ctcagagttg aacggcaggc tcacgaccta
    11701 atcagaaaaa cgattaaaat tcattcacaa agaaatcgaa taaacgctgt tcagatgaat
    11761 ctacacgatg agaaagttcc ggatgccatt ggacaccaag gattttgtgc atattatctt
    11821 tactttggac tgcttcaatg agtccatcgt tggaaaaggc gatggctttt aacaaaggag
    11881 aaagatcttt gactgcctga tgatgatagg aattcacaaa gtatgaatca gggagaaggt
    11941 cacttaataa actgtccgaa acgattttga cttcgtgagt tgcaaactgc ggggcagtgg
    12001 gctgttgttc gtgttttacg gaccattttg gatataatga aagatcttga taaagtgttc
    12061 cctccaacac gacattcaat agttgaagtc ctcggcagac tgcaaaaatc ggttttcttt
    12121 gtttcaatgc ttccaagatc aatgcctgtt cgaattggtc tcgttgaata tttgtttccg
    12181 tcagttttgg atgagggtct tccatgtaaa gttgcggact aacatcctga ccgcctgcaa
    12241 gaatgagttt gtcgattttc tcaattagtt gaggcgctga atctggagac atgactggca
    12301 gaataagagg taatccttga gcatctaaaa ctgcatccac aaatccctgc ggtgtatagc
    12361 ttacttgatt tccttggaaa gtatctgtcg ctcgaatcag ttgattcccg gcaatgccga
    12421 tgattggttt catttttatt ccccctaaag tagactaatt gtaacaagga tttaaaaaaa
    12481 tttaaactat gtaagacttg cccctatact tatctgttct tgctgtgtta ctatctgtaa
    12541 agagaatttt cgtgtgaaat tcatgacgat agttgaaaat attgcttaat ttgttataat
    12601 aaaaagtaag cagaaataaa aaggttgggc gtaagcttca aggatctagt tcttcaaggg
    12661 gtgagaagga cattttattg aaggggcctg cctatactaa gttatcttta gaggtacata
    12721 ctttcaatga aaaaagaaag catgtacctc ttttttaaat cgaaaggaag tgaagcaagt
    12781 gttagaagct ttggaacaaa tccgaaaagc agaagaagaa aatgagaagc gtgctcgaac
    12841 cctacggcag cagttgaaga cctatgcaga agaaaaagag caagaattgg agataatcca
    12901 aacgaacagt cggcaacagc tgaaacagtt ggtagaagaa aaagaacaat tcgaaaagca
    12961 agaattagaa aaagaaaaaa tgaaattgaa agcagaagca gacaaggtgc aagcaacttt
    13021 gaaagaccaa tatcaaaagc atcagagtca agcaatcggt gcaatcattg aaagggtgaa
    13081 agaaacttat ggccgtcact aagatggaaa aagtgaccct catttcagat aaaagaaatc
    13141 aggagaggat tttacaagca gtacaaggaa tccaaaatgt tgaaatccgg gatctgtatc
    13201 aaaatagaac aaacaaccag tgggtagaaa aatattttcc agataccacg attattgata
    13261 aagaagcaaa attaagcgct ttaggcaatc ggctgaaaga catccaagaa gctatccaat
    13321 tcatacaaca ccacggcgat agccgtcaaa agaaactcca tttgaaacat caagaacttt
    13381 ccttacaaga cttggaagca aattattccg aagaaaattt catgaataaa ttagaagaag
    13441 tccttgattt aaaagaaaag tgggaacagc taactgaaaa acgtgatcgt ttagaagaag
    13501 aggaagaatg gcttcttcat tggcaacagc tggatattgc tccaagtatg tatgcaagta
    13561 agacgaccat ttttgagatg gggactgtga gtgcagcaaa ttttgaaagt ttcaaggaag
    13621 aactaagtaa gcttgatgac gtttatatgg aagaaataga ttatacgcca aaacaagttt
    13681 atctttcgta tgttgcttta aaaaaatcag ttgctgctat agaagaaatt gctgggaaat
    13741 acgggttcaa taaagaaacg tatccttatg aacaaaatcc taaagaaaaa ttgcaagaaa
    13801 ccaaacagca attatccatt gttcacgagc agcaaaaaaa gataacgaca gcgttaggtc
    13861 agtgcagcgg ctatatccaa gaatttgaat gggtagaaga agtcacactt gccgttgcag
    13921 aacgggaaaa agtaaaagaa cggtttatcc atgccactta tctgatcgtt ttgcaaggtt
    13981 gggtagatgc agaagaaaaa cagaatttga tgcatgtgtt aaacgaaact gtatcagaag
    14041 aagatatata cgttagtttc gaaacaccaa actcagaaga gattcagcag gaagtcccta
    14101 ctaaattgaa aaaccatccg cttatcgaac cttttgaatt gctgactgaa atgtacagtc
    14161 tgccaaaata tgaagaagta gatccaacgc cttggatgac accattttat cttgtcttct
    14221 tcgggatgat ggtcgcagat gttggttacg gattgctgat gctcattggg acaatactag
    14281 ctcaaaaact atttgtttta ccccgcggga tgaaacgttt cgtaaaattc tttgagattc
    14341 tctcggtacc atcgatcatc tggggattga tctacagttc atttttcgga caggcattac
    14401 caaaagaact atttggtatc cgcttgcctt tcccaatcct atctacgacg gatgacgtca
    14461 atacgatttt gattttatcc gtgattttcg gtctgataca gatcttggtt ggattattcg
    14521 tggcagcaaa ggaaaatatc aggaggaaag attacttgag tgctgtcagt gatggttttg
    14581 cctggcaagg gatcctgatt gggatcgtta ttgcattgat tggtgtcatg ttgataaaaa
    14641 atacagcatt tgtttatctt ggcgcggggc tatcgatcat tgctgcttta tgtatcttaa
    14701 tcgtgccgat tatccaatcg ccgtctaaag tcaagggtgc tgccaaagga gcgtataatc
    14761 tttacggatt gactggttat atcggtgatt tagtcagtta tacacgtttg atggcactag
    14821 gtatttcagg cggaagtatt ggcgcggcat tcaatatgct agttgctttc atgccgccag
    14881 ccgctcgatt cagtgtaggg atccttttga tcatcgcttt acacgcactg aatatgtttt
    14941 tgacactgtt aagtgcctat gtccatggtg cacgattgca gtatgtcgaa ttttttggaa
    15001 aattttatac aggaggcgga cgtgcctttg acccgctgaa aacggcggaa aaatacgtca
    15061 acatcaatca taagaaaaaa aacaataaat aattggagga tttttataca atgatggatt
    15121 acttaattag tcaaaatggt ggaatggtat ttgcagtatt agcgatggca acagcgacga
    15181 ttttttcagg gattggttca gctaaaggtg ttggaatgac aggtgaggca gcagcagcgt
    15241 taacaacaag tcaacccgaa aaattcggac aggctttgat tctgcaattg cttccaggta
    15301 cgcaagggtt gtatggattc gttatcgcct ttttgatttt tatcaactta ggaaatgata
    15361 tgtcagttgt acaaggattg aactttctag gcgcttctct gccgattgct tttacaggtt
    15421 tgttttcagg tatcgcccaa gggaaagttg cagcagcagg tatccaaatt ttagcgaaaa
    15481 aacctgaaca tgctacaaaa ggaatcattt ttgctgcgat ggttgaaaca tatgcgattt
    15541 taggtttcgt tatttccttc ctattagtat tgaatgccta agcataaagg aggaaaagca
    15601 gatgaacgcc attgaaaaaa tcatctctca gatgaatgaa gcagcagagc aagaaagagc
    15661 ggcgttagag caagaagagc gaatgaagat cgatcaaaac tttgaacaaa aacgtacgca
    15721 aatcgaaaca gaacatcaaa aacaaaaaga aaaacaaatc gaattacttg aaaaaaaata
    15781 ccgtcagctt aggaatcgcc aacaagtaga agtacgacaa gaaaatttga atgcgaagca
    15841 ggaattttta cgacgattgt ttgcagatgc aatagctgaa atggagaatt gggatgaatc
    15901 tgaacaaatc cagtttatca aaaattcatt gtattcatta cctttgacag gcaaagtaac
    15961 attcattgct ggagaaaaat cagcagctta tctttctcag acattgctgg atgaatggaa
    16021 caatgaactt ccctttatga tggttttaag tgatgaaact gttgctgatc aagcgggttt
    16081 tctgataaac gatcaaggag ttcagtataa ttttcttttc agcagccttg tacaagatat
    16141 ccaaggaacg atgagttttg agattgccaa tcaattattc gaataaggag ggatcagtgt
    16201 atggaatacc acgaactaaa tcccttgatt cgcggaagag agcttgaact tttgtccaat
    16261 gagacatttg aacggatgat tcaaacaaat tctgtcgaag cacttggtga aatattgaaa
    16321 tccacgattt acagtccata tatttatgac ggatatgaat atgacttcga acagaatctt
    16381 ttaaaggagc agagccgtct atttcgctgg ttaaaggaat cagcaccgga gcctgaaatc
    16441 gtttggatct atacgatgcg ctatactttc cataatctga aggttttgac aaaagccgag
    16501 atgacaaatc aaaatctgga ccatttgtat atcgacgatg gattttattc attagaaacc
    16561 ttgaaggatg ccattcatac gcaagtttca aatgaactcc ctgaaagcat tatggacagt
    16621 atacgagaag tacgtgatta ttttgaagag tctaccattc ttcagggaat tgacgtgatt
    16681 tatgatcgac agtttttgac agagcagcga cggcttgccg ataagctagg atatcaagaa
    16741 ttgctgacag aagtcattgc ttttattgac ttgaccaata tcacaacgat ggctcgagga
    16801 atattgcaga aaagaacata tgggttcatg acgacggtac tttccagctc gggaagtatc
    16861 gaaaaagaag tatttttgaa ctttgtagat aaagatatgg aagattacac gaattttcta
    16921 ttagagacag attatgctga ttttctgcgc cctgtcatcc gtgagggaaa tcttgatctc
    16981 gtaaagcttg aacagttgaa agatgattat ctaagttcat tgtatcaaga agcacaaacc
    17041 caggctttcg gaccactgcc actactagcc tttttgaacg caaaagaggt agaaagcaaa
    17101 aaccttcgtt tactggttat cggcaaaaga agccatttcg aaccagaaac aatcagaaag
    17161 cgggtgagac aagtctatga cgcataagat cggtgtagtt ggcgacaaag attctgtttt
    17221 gccattcaaa ttgtttggct ttgatgttcg ttatggtaca accaagcaaa ccgttcgtca
    17281 ggcaattgaa gaaatggcaa agaatgagta tggcgtgatt tatgtcacag agcaatgtgc
    17341 agcattgagc ccagaaacga tcgaacgtta caaggaacaa atgatacctg ctatcgtatt
    17401 gattcccaat catcaaggat cattagggat agggctggaa gaaattcaaa aaaacgtaga
    17461 aaaagcagta ggtcaaaata tcttatagta tgaaaaggag atagaaattt tgcaaattgg
    17521 aaaaatcata aaagtctccg gacctcttgt aatggctgag aacatgtctg atgccagtat
    17581 ccaagacatg tgtctagtgg gagatctagg agttatcgga gaaattatcg agatgcgtgg
    17641 cgatgtggcg tctattcaag tatatgaaga gacttctgga attggtccag gcgaaccggt
    17701 tcgctctact ggtgaagctt tatctgttga attaggacca gggatcattt cacaaatgtt
    17761 tgacggaatc caacggccat tagatacctt tatggaaatc acacaaagta atttcttagg
    17821 gcgcggagtt caattgcccg cattggacca tgagaaaaaa tggtggttcg aaccaacagt
    17881 agaagccgga gaaacggtga gtgctggaga tgttatcggg atcgtagaag aaacaaaagt
    17941 tataaaacat aaaatcatgg tgccaaatgg gataaaagga acgatcaaaa caatcgaaaa
    18001 aggttcattc actattgatg aaacagtatg tgtcattgaa accgaaaatg gtgacaaaaa
    18061 tttgacgatg cttcaaaaat ggccagtaag acgtagtcgg ccaatcaaag aaaaattaaa
    18121 cccggatgca cccatgatca ctggacagcg agtgattgat acattcttcc cagtgacaaa
    18181 aggaggagca gcagctgtac ctggtccttt tggtgcaggg aaaacagtcg ttcagcatca
    18241 aattgcaaaa tgggccgatg tggacatggt tgtctatgtg ggatgtggcg agcgtggaaa
    18301 tgagatgacg gatgttttaa atgaattccc tgaattgatt gaccctaata caggagaatc
    18361 gttgatggaa cgtacggtat tgattgctaa tacatcaaac atgccagtag cagctcgaga
    18421 agcttctatc tatacgggaa tcacaattgc ggaatatttc cgtgacatgg gttatgacgt
    18481 tgctatcatg gcagactcca cttcacgatg ggcagaagca cttcgtgaga tgagcggacg
    18541 tttggaagaa atgcctggtg atgaaggtta tccagcttat cttggttcac ggcttgcgga
    18601 atactatgaa cgatctggaa gagtcattgc attaggatca gaaggtcgcg aaggaagtat
    18661 aaccgcaatc agtgctgtat ccccgtctgg aggggatatt tcggaaccag tcacacaaaa
    18721 tacattgcgg gtcgtaaaag tattctgggg tcttgattcc agtttagccc aaaaaagaca
    18781 ttttccttcg atcgattgga tacaaagcta ttctctttat tcaacagaag tagggaagta
    18841 tatggatcat atccttcaag ctgactgggc atccatggtc accgagggca tgcgtatttt
    18901 gcaggaagaa cagcagctga atgaaatcgt gcgtttggta gggattgatt ccctttcaga
    18961 taatgaccgt ctgacattag aagtggcaaa atccattcgt gaagattatt tgcagcaaaa
    19021 tgcttttgat gatgtcgata cattcacttc aagagagaaa caattcaaaa tgttgaagat
    19081 gatcttgacc tttggtgaag aaggacgccg tgcgttgtcg ttaggtgctt atttaagtga
    19141 gatcatggac ggtaccgtat cgattcgtga aaggatcgga cgaagcaaat atatcccgga
    19201 aacagaatta gaaaaactag atcaaattat tggtgaaatc aagcaaacga tgcaaacgat
    19261 cgtttcagaa ggaggcatga gcaatgatta aagaatatcg tacgatcggt gaagtcgtcg
    19321 gtccattgat ggcggttgaa aaagttgctg gtgtcaaata tgaagaatta atcgaagtcc
    19381 gtatgcaaaa tggcgagatt cgtcgtggac aagtattgga agtccaagaa gataaagcaa
    19441 tggtccaaat attcgaagga acaagtggta tcaatttacg tgactcttct gtacgatttt
    19501 taggccatcc tttagaacta ggtgtatcgg aagacatgat cggtcgagta ttcgatggtt
    19561 tgggacgacc gaaagataac ggaccagaga tcctgcctga aaagcatttg gatattaacg
    19621 gagaagtaat caatccagta gctcgggatt atccagatga gtttatccaa acaggtatct
    19681 ctgcaatcga tcatttgaat acattagttc gtgggcaaaa attaccagta ttttctggct
    19741 ctggtcttcc tcataaagaa ttagctgctc agatcgcgcg acaagcaacg gtactcgatt
    19801 catcagatga tttcgccgta gtctttgctg ctattggaat cacttttgaa gaagctgaat
    19861 ttttcatgga agattttcgt caaacgggcg caatcgaccg ttctgtcatg tttatgaatt
    19921 tagcaaacga ccctgcaatc gaacgtatcg ctactcctcg tatggctttg actgctgcag
    19981 aatatcttgc ttatgaaaag ggaatgcatg ttttggttat catgacagac atgaccaact
    20041 atgcagaggc attgcgagag atttctgcag cacggcgaga agttccagga agaagaggat
    20101 atcctgggta tctttacacg aacttagcta ctttattcga acgagcagga agaatccgcg
    20161 gactaaaagg atcggttact caaattccga ttctaacaat gccagaagac gataaaactc
    20221 atccaatccc tgacttgact ggttatatta cagaaggaca gatcatcttg actagagagc
    20281 tttacaagag tgggattcag ccaccaattg atgtactccc gtctctttcg cggttaaaag
    20341 ataagggaac tggacccgga aagacaagag aagaccatgc agctacgatg aatcagcttt
    20401 ttgcagccta tgcacaagga aaacaggcaa aagaattagc cgtagtttta ggtgaatctg
    20461 ctttatctga tgtagacaag atctatgcga agtttgctga gcggtttgaa aaagaatatg
    20521 tcaatcaagg attctacacg aaccgttcga ttactgaaac acttgatcta gggtgggaac
    20581 tgctatcgat gttgccaaga accgaattaa aacgaatcaa agacgacatg ctggacaaat
    20641 atctgccaga agggaagtga gttctgatgg cacgattgaa tgtgaatccg acccgcatgg
    20701 aattgactcg attgaaaaaa cagttgacaa ctgccacaag agggcataaa ttattaaaag
    20761 ataagcaaga tgaactgatg cgtcaattca tcctgttgat tcgaaagaac aaccagctgc
    20821 gtcaagaaat ggaagagtct atgacagaag caatgtctga ttttgtattg gcaaatgctt
    20881 cgatcacgga acctttcatc gaagaattat ttgttttgcc ggcggaaaac gttgatttgt
    20941 ctatcgtaga caaaaatatc atgagtgtaa aagtacctgt gatgaatttt gaatatgatg
    21001 aacagctgac agacacacct ttggaatacg ggtatttgaa ttccagtgcc gaactggatc
    21061 ggtcaatcga tcggtttact accctgcttc caaaactttt ggaactgaca gaaatcgaaa
    21121 aaacttgtca actgatggcg agtgaaatcg aaaagacacg tcggcgagtc aatgcattag
    21181 aatatatgac gatccctcaa ttagaagaaa cgatctatta tattcgtatg aagctggaag
    21241 aaaacgagcg cgcagaagta acaaggttga tcaaagtaaa aaatatgggt accactgact
    21301 aagtggtatc tttttttaaa agaggaaaac gatgattaaa caaagcaaac aaataaagaa
    21361 acgtttgccg gctgttcagc ttctcgcatt aggatttttt ctcctgattt tagttggcgg
    21421 cggcctactt accctaccat tttttagtaa tagtgggcat gggacaaatt ttatcgatgc
    21481 gcttttcact gcaacttctg ctgtctgtgt aacgggattg acaacattga atacagcaga
    21541 gcattggaac agtgctggtc aatttttgat catgcttttg attgagatcg gcggtcttgg
    21601 ttttatgatg gttccgattc tatgctttgc attgataaag aagaaagtca gtctcagcac
    21661 acgtattata ttaaaagaag cgctgaatct agaagaagta tcaggtgtga tgaagctcat
    21721 gctttacatc ctaaaattgg cctttgttat ccaattgatt ggtacggttg ctttatcttt
    21781 tgtatttgtg cctgaatttg gctggttgaa aggtatctgg tacagtttgt tccatgcaat
    21841 ctctagtttt tgtaatgcag ggtttgattt gttaggagac agtttagcca atcaccagca
    21901 aaatgtttat ttgatcatgg tggtatctgc tttgatcatt tctggtgggt taggcttcat
    21961 cgtatggcgt gatttactgg aatatcatca gaaaaagaag atgacgattc attcaaaaat
    22021 cgcattaacc gttacaggta cccttttatt aggcggattc gttgtgttct ttttcacaga
    22081 gcgcaatggg gcaacgctag tggaagggaa tatgtttcaa cgattggcaa acaccttttt
    22141 tatgagtgtg acacctagaa cggctggata ttattcagtt gattacctcc atatgagcca
    22201 tgcaggtctg attttaacga tgtttttgat gtacatcggc ggaacatctg ggtcgacggc
    22261 cggcggattg aaaacaacta cgttaggtgt gttgctgatc cagatgcgtt cgatgttgaa
    22321 aggacgtacg agagcggaag catttggccg taccatccga caaggcgcag tgttgcgggc
    22381 acttacttta ttttttgtta cgttgacgtt gtgtgtagtc gccatcatga ttttatcggt
    22441 gactgaaacg atacctaaaa cttctggcat cgaatatatt gcttttgagg tcttctctgc
    22501 gtttggtaca gtaggtctta cgatgggatt gacgccagac ttgactgcaa tagggaaatt
    22561 gatcattata gcgcttatgt acattggacg agttggtgta ttgactgtgg tcttctctct
    22621 gatgacaaaa gcacaaagaa cacaatcaaa atacaaatat ccagaagaaa gtgtcatgat
    22681 tggataattt catcttctga cagaaaccta gtgatttagt aaaataagaa aataaaataa
    22741 tttttcttgc ttcttggata ctttaagtgc aaaatgttcg tttatttttt gaggcactac
    22801 ccgttgttga acctcgggta gtgcctcttt tttattgaaa ggaatcaata agatttgatt
    22861 cctttattta tcaataggtt ataattcata tttttttatt ttttctttta cgatacttag
    22921 tccagaagct aaaatttcaa ggttattaga ggatagggaa attcgaacga atgcatctgg
    22981 ttgcgcacgt gtagtaaagc gtctggaatg ataaagtcgg ataccgttag caagaaagtc
    23041 attctcaatt atcttagggt caatctcagg atcaactgga agaattcgat agtaagggac
    23101 acgattcgtt ggtttttcta ctttgaaaag agaatcaaaa agctgattgg ctctttttgt
    23161 aagacttaat ttagtttcta agatcctatt tgcttcattt gaatggagga tttgagtgat
    23221 gacttccaca tctaatccag aagttttcac atttatatta aaaattgctt gttctatctt
    23281 gctttggaca tgtactggaa ctacaagata agcaactcgc aatccagaac aaataaattt
    23341 tgtcattcca gctaagtaga ttgtttggtt aggtaataaa ttttggaaag gaggaatgat
    23401 cgtatgttga tagtaagttg ttaaaaatga atggatatca tcttcaataa cccatagacc
    23461 ttctttttca atcacttcgg ctaattgctc tcgtcttttg tgagagattt gaaaccctat
    23521 aggattatta caagaaggca taagaaagac accatgaatc ttcttttttc tacattcctg
    23581 aatcaatata tcaggtcgca ttccttgctc gtcaaaatca attggaacga tctctaaatg
    23641 atataactga gccaattcaa tgaaattagc gtatgtatat cggtctgttg ctattctatc
    23701 acctggagag aaaagtgcag aaagtgtgat agccaatcca ttttgaacac cggaaacaat
    23761 agatagagat tctaaggacg gaatgactcc ttgagttttt aaccaatttg ctgcagtagt
    23821 caattgaaaa tttgtgcctt tgggatcttg gtaattaagc aaaccaacaa gatttgggtt
    23881 attactagtt gattgaataa acgggacaac gaatttatta cattcttcaa aagaactaac
    23941 cagaccaaaa tcaatgatat gttcggttac ttcattggta gagatggtga tggactcaaa
    24001 gacattggga gaattaaatg tgccacttcc aatattcgta tacagcaaac ctttctcaat
    24061 tccatattta taagccttgc caacggtagt aaaatttaaa tctaaataat cagctagctc
    24121 tctttgggag ggtaactgag tgtttttctg taatttattc tttaaaatat cttgttctaa
    24181 ttgttgaatc aacgatcgat aataaggacg cttcaatttt tgtttgtctg gtcgccaagt
    24241 taacgggtaa ttgtcataag aattaatagg cattttctcc actccttaaa attgtatacc
    24301 atacaatttt accattgatt gtattgattt tagaagttaa tcttttgtga ggagggaatt
    24361 gtctatgaaa attattaatt ttgaggaagt agtagcttct tttagttttg aagaagcgat
    24421 tcaagtaatg aaaaaatgtt tttttgatta tgaaagtggt aaaatcagcc aaaatcctcg
    24481 attggtggaa attttacctg acggaaaagg caaaaatatg tttgcgatga tgcctgctta
    24541 tttgggggag aacagatatt ttggcacaaa agtcattacc gcttttccgg ataatcatca
    24601 gtatcattta ccttctcatt taggagagat tttcttgttt gatgctcaaa acggactacc
    24661 caaagcattg ataaacgcaa atgcagtcac ctggattcga acagctgctg tatctgcgct
    24721 ggcaacagat tacttagcaa atccgcatgc agaaacactt gctttaattg gagctggtca
    24781 acaagcattg tcgcatttga ctgcgatttc taccattcgt cccatcacta cagtgtttgt
    24841 ttacgatatt ttagaagaaa gacgaaatac ttttatcgct aaagctaaaa aacagtatcc
    24901 taaaataacg tttataaatg caatatcagt agaagatgca gtacgtccag cggaaatcat
    24961 ttgtacgttg acctctagta aagaggcatt tcttcaaaaa gagtgggtaa gggaagacgc
    25021 acatatcaat gctgtgggca catttactcc tgatacaaga gagataagta catcgttgat
    25081 ggaaacttct caaatctatg tagatgatta ctcagctgcc ttaacagaaa gcggggacct
    25141 gcttatagcc atggcagaag gagatttaga ggaatcttgt attgttggta cattaggcga
    25201 attagtttct gagaaaaaaa cattttataa aggagaatcg aggccgacga ttttcgacgc
    25261 agttggtcta gctattgaag atctttgttg tgcagaatat atttataaca aattagagaa
    25321 aggggcttta aaatgaactt agattattac gtagttgatg catttgcaga tgaggtgttc
    25381 aaaggaaatc cagcagcggt ttatgtgcta gaagaatggc tgccagaagg tacgatgcaa
    25441 aaaattgcca ttgaaaacaa tttatctgaa acagcattta cagtgaagaa aaaccaagaa
    25501 tttgagctta gatggtttac gccagatcga gaaatcgatc tttgtggtca cgcgacatta
    25561 gcaacagcat ttgttttatt taactattac aaaatacctg atgaaacgat taaattttct
    25621 actcaaagcg gtaatctatt tgtgacaaga attaaagatt attattacat ggattttcct
    25681 agtattatgc cgaaaaaagt gccgatattg tctgaatatg aagaagcgat tggggctaag
    25741 attaaagaag cctacttagc acgtgattta tttttcgtat tagaagatga aaaaacggtg
    25801 gctaaattgc agccagattt tacagctatc aaaaattttg aattaggaat aggagtcatc
    25861 gtgacagcag aaagtgtgca aaaagacttt gtttctagaa ccttctttcc aaaattaaca
    25921 atcaatgaag atccagtatg tggatcagca cattctaatc ttattcctta ttgggcagaa
    25981 agaatgaata aaacaaaatt gtcagcttat caaatgtcat caaggggcgg ggatctagaa
    26041 tgtgaattaa aagaagatcg tgtgataata ggaggaaaag cagttttgtt ttctaaaggt
    26101 acctcgttta tttgatttct ttcaaatgaa ttaaaaataa attggtggag agtggcaacc
    26161 ttatagctac aatggttgtt aaaaaaagac gcaaactttt tcaatttaat gaaaagtttg
    26221 cgtctcattt taattattgg tgtttgtttt ttattcaatc aagagtaaaa tgccccatac
    26281 taactatcct cggatgactt cgattttata accatcaggg tcaatgacaa agtagtaaga
    26341 aggcgcagtg ccaggtaatc cttttaagtc agtaatctca agaccggcag ctttatgttg
    26401 ctcgtgtaat ttttctagat catcagctgc tatagcgata tgaccatagc cgtctcctag
    26461 atcatacgca ccatgatcat agttgtaagt caactctaat tcatagtcat ccccaggaag
    26521 tgtcaaataa acaagtgtaa atttgttttc tggaaaatca cggcggcggc tttcttcaaa
    26581 accaaatgcc ttttgataaa attcgattga tgcttcaaga tctttcactc gaacacatgt
    26641 gtgagccatt ttcatagatt catctctcct tttcctttta ctcaatcata acaaaaagaa
    26701 gtaaggaaat agttgatttg tgcttgttca tgagaaattt ctcatgtaaa atggatagaa
    26761 aataaaactg ttaaggtgga aactattcat ggataaacca atttctggtg aaaaaaagca
    26821 atttgctgat tatcaaaccc gttatgcagc atatattgtc atagaaagag atcagcagat
    26881 tgcagtaatc gaggctccaa atggtgcttt ttttcttcct ggcggggaaa ttgaaggaga
    26941 agagacgaaa gaagaagcga ttgtccgtga gctgatagaa gaaatgggaa ttgtggcaga
    27001 aatttcttct tatctgggag aagctgatga gtatttttac tcgaattatc gtgcaactta
    27061 ttattataat ccaggatatt tctttgttgc tgattcttgg aagcgtatag gagaaccaac
    27121 tgaaaagacc aataaaattt ggtgggtgag ccctcaagaa gcgcttgtga aattgaaacg
    27181 tggtagccac cgatgggcag tcgagagatg gatcgaaaaa aataaaaact gaaagaaagc
    27241 gagtagatca gctttctctc agttttttta tttattacag tacatatttt ttgattgctt
    27301 cagctacacc gtgctcgtcg tttgatgaag tgatgacatt tgctgcttct ttgacaagag
    27361 aaacggcatt tcccattgct actcctagac cagcatattc aatcatggat aagtcgttct
    27421 cgttatctcc gatagccatg atttcatctt gatcaatacc aaggtgctgt gcgagattcg
    27481 caactgccgc acctttactt gcttctttat ttagtacttc gaagtaatag ggcgcacttt
    27541 tgaccgttgt gtatttatca cggaaagttt gcggtaaacg cgcaattgct gcatctaaaa
    27601 tttctggttc gtcgatcatc atcattttga cgatgttcat gtctggtgtc atttcttcag
    27661 gtgttcgata ttttagcggc atcttgacta aacttgcttc gtggatcgta taagcgctga
    27721 tatctcgatt aggcgtgtag atcgctttgt catcgatcga atggagatga ctgccgacac
    27781 ggcgtgccat cacttcgatc tctaggaaat cttcatgtgt cagtccgtga cgggcgatga
    27841 ttttacctga tttcgtttgt tgtacgagtg ccccgttata agtaataacg taatcattat
    27901 cttgaaaaag gttcaactcg tttaactgtt cctggacacc tggtaaggga cggccagtgc
    27961 ataaaacgat cgaaacacct tttgcaacag cagcggcaat cgcttctttt acttcttttg
    28021 ttacttctct tttctcattt aataaagtac catcgatgtc gatagcgact agtttgatag
    28081 acataatttc ctccaatata ttttaattta tttcaatcaa tgcgccatta cggatatggc
    28141 tggcaaattc ttgataagtt tcgtcaaata aatcataatg atcacgcaag gacggatcaa
    28201 ccatttcttt tggaaagaaa aatcgctcat ctccttgttc ttgtcccgct aatgcagcaa
    28261 caagctgact aacctgtgat aattctatca aagttccgtc atttcgcaaa atctcgattt
    28321 gtgtgcgatg tctgccttgt tctgggcgat aaaaatcata aggtagatcg tagctagaat
    28381 tcacagcagt gtaataaaca ggattatatc cgaccttttc tactaacaaa gtgagttctt
    28441 ggactaattt tgaatcccgg ttgccagaaa aagtagcaga tttcagtggt ttacgattca
    28501 aaaaacgttt agctaaatca tttaaaatag gatcagcaga ttcagaccat tgagtaaagt
    28561 aagtgcccaa gaccccatca tcaagtttga ggtattcttc taaagtaaag cttccgtcaa
    28621 gaaatggcag aagtaattga gaatggagtt caaaactgct ggcatcttct tgatacaact
    28681 cgtgtgcgcg atgaagcaga tgctccaaaa ttacttccat accacgggaa acaggatgga
    28741 agtacacttg cacatacatt tgatatcggc taacgatata atcttccact gcatgcatgc
    28801 cactcatgga aaaagcgatc ccgccttcat aaggacgaat cacacgtaat attcgggtaa
    28861 gatcaaatgt tccatattct gttccagtga aataagcatc ccgaagcaaa tagtccattc
    28921 gatctgcatc gatctgacta gaaatcattt ggacaacttg tgggttgggg taagtttttt
    28981 ggatcacact agctaccttt tcaggaaaac cttcttctac tcggttaaga atctgataca
    29041 cttctgtttc tggagaagtg atgatctcta cagtgatggc ttcatgattc gtatggaaga
    29101 tatgttcaaa tgtatgagag taaggaccat gaccgacatc atgcaacagt gccgcacata
    29161 aagtcaccag ccgttcctga tcatcccagc ctccgttgcc tattttttct tttgaaaaat
    29221 ttcgagaaaa gatatcgcag attcgtcttg aaatctcata aacgcctaat gaatgagtaa
    29281 aacggctatg ttctgctccg tgaaaagtga aagaagaagt gcctaattgt ttaatgcgtc
    29341 gtagacgttg gacttctttc gaattgatca aatctaagat tacttgatgc tggacatgga
    29401 tatagttatg gactggatca cgaaaaactt tttccatagg cagcatttga tatttataag
    29461 gggttgtcat aatgtagctc ctttatgtta ttacgttgtt ccattctctg gatttggttg
    29521 ttccattgct ccagctggat ttcttttttt agccagtcgg tagaatcctg taaatcactt
    29581 gcagaataca gcttttcaaa agcttttgca aatgcttctt tgacttcttt tacaaaaagg
    29641 ggttggtcaa gcagttcttc aagtgttgcc attgtagcag gatccactgc aggatagcca
    29701 tttgtaccaa agtcttcttt taaagcagat tgataaaatc gcttcatgac ttgcccgcgt
    29761 ttttcctgat taccgttgac acttaagtac atcataacag aaatcccatt tttgatccga
    29821 cgctgagcga taccggcaaa ttttttgcca tggatgctta aatcataagt tccaggacaa
    29881 taagaatggg tgatttcgaa tgcttgtatc ggtgcctttg gaaaagcgta tttagtcaat
    29941 aggagcattt gctcgtaagc gtcatctatg gttatttttt ttgtgtcact ttgggggaaa
    30001 atcaaagaaa tattcaaaat tccttcatct gatacgatcc ctaatccacc tgaatttctg
    30061 atcactggag cgtaacctgc atctttcagt gtttggatcc cgtcacttag aaaaggagtt
    30121 cgaacatcct tcatacctaa gatgactgtg cagtcatatt gccagaaatg taaaaagatt
    30181 tgctggtgtt ctttagaata ttccgttaat atatctgtta atgcaaaagg ggaaaagtcg
    30241 tttttttgat aaatgtgctg atctagtcga atcgcagggt tcattcaacc ggctcctttg
    30301 tataaattca tattttctac aatattatac tctaaaatca aagagttgtt ttggaaaaaa
    30361 ggttttctgt aaaaatggac aagttggttg acaaaacaaa agaaactagg aacaatgtag
    30421 ctatgagaag taaaggagaa tgaataatgg aattatcaca aggaacaccg atttcaatca
    30481 aattgcgtac aaaagtaaaa cagcaagggg aagtgcaaga tttttatttt gatttgaaag
    30541 ggcaaatggt gacaattggc gatacgctgt acatacgtta taaagagatt caggaggaga
    30601 cgggcgatga gattcctgta acgatcaaat tagttccaga tggtcatgta cagctgatcc
    30661 gtgcaggaga aatgcggatg cgtttgaaat ttggctataa agaacggtta gaaacatcct
    30721 atcgaacacc atacggtatg atccagattg ctacttttac gaaagaactg catgttagtt
    30781 tgaaagaccg tcccacagga gggaaggttc gaatcgatta tgatctgttc atgggaccgg
    30841 aaaaaatcgg cgaatattat ttgaccttgg attttacagc gtaaatataa aagaaaagtt
    30901 tgattttttg tgtctgattt tgtatgataa ggagtagact gtgaaaggac gtgtcctgat
    30961 tggaaattag tgtatttgaa ggtgcaaaca aaagcgaatt atcaatgatc gaagtagcac
    31021 acgcgatctt agaacaacgt ggcgatgtca tggatttttc tgatttagct aaccagatcc
    31081 aaaactatct tgggaaatca gacagtgaaa ttcgtgattc actcgcacaa ttttatactg
    31141 acttgaatat tgacggcagc tttatctcat taggggataa tcgttggggc ttgcgttcat
    31201 ggtatgctat cgattcgatc gatgaagaag taaaccatgg aatggacgaa gaggatgaag
    31261 atactccacg tcgccgtaaa cgcaagaaag tcaacgcctt tatcaatgat gatgaagacg
    31321 cgatcgatta caatgacgat gacccagaag atacagatct gacagaagaa gatgacgatg
    31381 atctgtttga tgacgatgat gatgaagatg aagaaatcgc tgcttataat tctgacttgc
    31441 aagaaatcgg agcagatgac ccgacagatg atgaagaaga cttgccagga aatatcgaag
    31501 aagatttaag tatcatagac gaagacgatg atgatgaaga ttatgaagag gaagaatttt
    31561 ctgactctga aaaagaataa acattatttt cttacgagag gttgtgacag agacgatcag
    31621 cctatcagaa taaggaggtg tgacacgatt tttgtcacac cttctttttt gtatataaaa
    31681 atcccaagta aatacataaa aaataaaaaa attgaaactt ttagcgcacc ttgtacgtca
    31741 tattaagtga ggcagaaagg agtggagaat gatttttact ggatataaga aaaaacggca
    31801 gatcgaaaaa taccgtcacc tgctgaatgg actcatcgaa actgattatc aaaaaatgtt
    31861 ccgtatcacg atgaattacg tacatgataa agaagaggcc ttagatgtga tgcaagatag
    31921 ttttcataaa gccctgaact attttgaaaa agaaaaagag atcgagcatt ttagtgcatg
    31981 gttttatcgg atattgatcc gcacggcatt agatagttgg cgcagacaaa aaaggacgcc
    32041 tgtggactta tttgaaattc aagatatccc ccaacagtta cctttagaaa cttccgttgc
    32101 agagctccat atgattttag ataaaataga cagtccggaa aaagagattc tgatcctcta
    32161 cttttttgaa ggatttcgtt tgaaagaaat tgcgactatc ttggatatga atgaaaacac
    32221 agttaaaacg aaaatgtacc gctcattgaa tgctttacgt caaatcctag aatgaaggag
    32281 agaagagtgc catgtattcc caaaaagaaa atttaaaaaa actaaagaaa acttataaag
    32341 aacaagcaat tcccaaaggg cttaaagagg agatccgcca gcgttttatt atcgaagagc
    32401 aatcatttat aaaagcaaag cggagaaaga gacgtctcca agggttcgga gtatctttgg
    32461 ctatcgtaat gattacaaca agtagccttc tttttaatag tcaagttcga agctttgcag
    32521 aagatttacc catcttagga tcagtcattg aattgatctt aggcgaacgc tttactgatc
    32581 gatctgaaaa aatcgatatt caggtaccga agatcaatac acaaaatgag aaagaaaata
    32641 aaacaattca tggattaaat caaaaatact ttcgcgaagg acaagcagat tttgaaaaag
    32701 ctgaaaaaga atatggaaat tttgaaacgg atcattacca agttttggga gattatcaga
    32761 aaatcgtaga cgataaccgg tttctcgtga ttgaaaggca gatcacccaa acagcagcag
    32821 atagccatgt tgaaaaaaga tacgacacaa tcgacaagaa aaacagtgtc cagctctcac
    32881 ttccactttt attcaaagat gacacctatc tatctgtttt gacaaaagaa gtcaaacggc
    32941 agatggccga acaagtcaag gaagatccat ctaagtacta ttggactgag caagatattc
    33001 aagaagggac gatagaaaaa ccaacacttg ttacgcctac acgaagtttt tatttgaaca
    33061 aagagcatca attagttttg actttttctc aatacgaaat tgctcctggt tatatgggta
    33121 caccagaatt tgtcattcct aagtccgtga caaaaacgat tttggcatcg gaagattatt
    33181 tggatcatta gacgagaagt agttgcgact agcagtttca attttcaagt aagtacaagt
    33241 aaataataag aagagaaaaa gagaaagaag agatatagtg aaaagaaatt atttagttat
    33301 cagtattatt gctttatccg tgcttttgtt tttcgaaatt acttcggtgg cctccttgat
    33361 caccgaagta aaaggacaaa cacactcttc ggagcattcg tcacaatctt tcaaacaatc
    33421 agaacaatca accattaaaa gccaatcaac tgaaacagct aacgtcaaag aagccattca
    33481 agaatcagcg gaaaaggtac aaagtcagtc atcacaatca acacaagaca gtacaaaaac
    33541 gagccgagat ccggctgtca aaagcgtcgc aatcagcttt gatgatggac caggagcgac
    33601 cacaacaccg cagcttctgc gaatcttaaa agaaaaaaac gtgcatgcca cttttttcgt
    33661 cttaggagaa aatacggcgc agcatcctga aatcgtgaag cagacagctg aagcagggca
    33721 cgagattggc aatcacacat acgatcatca gaatctggcc actctttctg cccagtcgat
    33781 gacggaggaa gtgacgaaag cagacactga gatcaaaaaa gtcactggca aaacccccac
    33841 ttttgttcgt ccgccctatg gttctgtcac gaatgtagga gcaacgatca tccagcgacc
    33901 gatcatcgaa tggtctgttg attcagaaga ttggaagaca agaaatcctg atttgatttt
    33961 gcagaagatc caagcaacag tatacgatgg ggcaattatt ttattccatg atatctatcc
    34021 tgaaacgatt cgtgccgttc ctcaagtgat cgattatctg aaagagcaag ggtatcgcat
    34081 cacgacagtt ggggatttat tagggcatcc aacggctgtc gagaactatt atggcagaaa
    34141 cgatcatcgg ccagttcagt aagtgagaca agtatcttta ataaataaca cattttaata
    34201 agtagttgaa cacataacta ccttacttgc aaaggcttaa caatatctat acaggtacaa
    34261 caaaaaactg tccaccaatt ttctttggcg gacagttttc acttaaaaat aaagaagcaa
    34321 ctattcgtaa attatgctaa aatagcagta gaacgcgtcc tgagggaatc gaacccccgt
    34381 ctcaagaacc ggaatcttac gtgatatcca ctacactaag gacgcaagca ctgggataat
    34441 tgtaactaaa cagataaaaa attacaaggg gattggacaa aaaaatgaaa tttgagcaaa
    34501 atttttcaca aaaacagcag cagacacaga aactagccat gacgcaacag ttgcaacagt
    34561 ccatccaagt tttacaatat aacagtgagg agctgttggc attcgttgag aatcaagcaa
    34621 tggaaaatcc attggtggaa gttgttgagc cggaatggca gccagattac ataaaagctt
    34681 cttcatcttc ttatgaagga gaagagacaa attacctaaa tcagattcct gatacaaaag
    34741 ggtctttatt tgattcattg atcgaacaag tccatttgaa ttaccgagat acttttttga
    34801 ggaaaatcgt tctttatctt gtagaataca tcgatttgaa cggatttttg acgattagtt
    34861 tagaagaagc cggaaaagag attggagcta cgcctatcca aatgctcgat gcgttgactc
    34921 tgattcagca attagaacca gctggagtag gtgcacgtag tttgcaagaa tgtctcatgc
    34981 ttcaaacaga acgagatgat tatgcgccgg aattagctta tatcgtgttg gaagaatgtt
    35041 ttgaggaact ggtggagcga aaatggaaag aaatcgcaca acgtttttct gttgatctac
    35101 atgctgttca gcagatattc gactatttac aaacgttgag tccaagccct ggccggattt
    35161 tcgaccgtag cagcgaattg tacattattc ctgatgtccg ggtattagtg gacgacgata
    35221 aaaatgtaca agttatctca aaccgaaaaa atcagccaaa tatacgcttt caagagagtt
    35281 acttcaaaca aatgtcacaa caagcagata aagaaactga aaattattta aaagaacgga
    35341 aacaggaatt cgaatggttg aagaaaacga tcctacaacg tggggataca attttgcggg
    35401 ttgctcaagt cattgtttcc cggcaaaaag ctttctttat cgacaaggaa cgtccaatca
    35461 agcctcttac gttaaaggaa gttgcaactg agatagatgt gcatgaatcc acggtcagtc
    35521 gtgcagtcaa cgggaaatat ttggaaacag acttcggtgt atttgaatta aaaaaatttt
    35581 tcactacacg aattcctacg aatagtacag aacagacaga agacctttca gcagatacag
    35641 cgaagaaaaa gctgcaggaa ttagtcgatc aagaagacaa aaacaagccg ttgtctgacc
    35701 agaaattggt tgaattgctg aaaaaagatg agatagcgat ctcaagacga acagtagcaa
    35761 aatatcgtga tttattagga atcccgagtt catcaaaaag aaaacgttat gataactaat
    35821 cagtcaaaag actagaaaaa gtatttcact ttttctagtc tttttagttg aaaaacgaga
    35881 gcaaaactgt tagacttaca gtcgtgaggt tgagatttcc ctttattgat gtgaaattcg
    35941 aatagttaat ttcttttttt atcgtatatg ggtcgtttta agtctacatt ggacgtaaag
    36001 tgtccaacta aggagatcgc tatgctggat gaaataaaaa tgattgaatc tgttgcacca
    36061 gatattatcg aagtgttgca agaacgctat aaaattttac gaaatatcta ttggatgcag
    36121 ccgattggac gtcgaagttt atcggaaagt atggggatca cagaacgagt attgcgaaca
    36181 gaaacagatc ttttgaaaaa tctgaatttg atcgacacct caaaaagcgg tatgacgctg
    36241 acaactaagg gcgaagaagt ttatcaaagc cttgaaaact tcatggatca gcttttaggt
    36301 acgcatcaaa ccgaacaaca gttggcagaa tattttggta accaacgctg tatcgttgtt
    36361 tcaggaaaca gcgaagagca gaccaaagtg gctgatgcat tcggcgaagc gctgagcgaa
    36421 gccctagacc gactacttcc agaaggcgag aatatcatcg ctgtgatggg aggaactact
    36481 atggctgtgg tagccgaaca attgtctaat ctggaaaata agaaacggca taacctgttt
    36541 gtccctgcaa gaggcgggat tggtgaagcg ataactgtac aggcaaattc tgtcagtgca
    36601 agaatggctg ctaaagcaaa cggtaaccac cgtgcacttt atgttccgga acaattaagt
    36661 ctggcaactt acaattcttt actcaatgag ccatctatcc aagaagtctt aaacttgatt
    36721 agtgaagcga attgtgttat tcatagtatt gggcgtgcat tacacatggc agcacgtcgt
    36781 aagatgactg aaaaagaatt agtcatgctg aaacaagcaa atgctgttgc ggaatctttt
    36841 ggctactttt tcaatgaaaa aggtgaagtc gtttataagg tccctagaat cggcatagaa
    36901 ttgcaagatt tggaaaaagt cccgatcatc atggcaatcg cgggcggaaa atcaaaagcc
    36961 aaagcaattc gagcttatat gaaaaatgca ccaaaacaaa cgtggctcat cactgatgaa
    37021 gctgctgcaa atgagatttt aaaaggggta accctttaaa ataaatattt ttttgatttt
    37081 cataaggagg aaatcttaaa tgacagttaa agtaggtatt aatggttttg gacgtattgg
    37141 acgtctagca ttccgtcgta tccaagatgt agatggaatc gaagtagtag caatcaacga
    37201 cttgacagat gctaaaatgt tggcacactt gttaaaatac gacacaactc aaggacgttt
    37261 caacggaaca gttgaagttc atgaaggttc attcaacgta aacggaaaag aagttaaagt
    37321 attagctaac cgcaacccag aagaattacc atggggcgaa ctaggcgtag atatcgttct
    37381 agaatgtact ggtttcttca cttctaaatc agcagctgaa aaacatttaa cagctggtgc
    37441 taaacgcgtt gttatctctg ctcctggcgg aaacgatgtt ccaacaatcg tttataacac
    37501 aaaccacgaa acattaactg gtaaagaaac agttatctca ggtgcttcat gtactactaa
    37561 ctgtttagct cctatggcta aaacattgaa cgacaaattt ggtgttgttg aaggattaat
    37621 gacaactatc cacgcttaca caggtgacca aatgactcta gacggacctc atcctaaagg
    37681 tgacttccgc cgtgcacgcg ctgctgctgc aaacatcgtt cctaactcaa caggtgctgc
    37741 taaagctatc ggtttagtaa ttcctgaatt gaacggtaaa ttagacggag ctgctcaacg
    37801 tgttcctgta ccaacaggtt cattaacaga attagtaaca gttcttgaaa aagaagtaac
    37861 tgttgacgaa atcaatgcag caatgaaaga agcttcaaac gaatcttacg gatacaacac
    37921 agacgaaatc gtttcttcag atatcgttgg tatgacttac ggttcattat ttgatgctac
    37981 acaaactaaa gtaatgacag ttggcgacaa acaattagtt aaaactgttg cttggtacga
    38041 caacgaaatg tcatacactg cacaattagt acgtacttta gaatacttcg ctaacttata
    38101 agatagacaa acgtacaagt gtaaattata gacatgtaca tttaaaagcg gggaagcaat
    38161 cgcgcttctt ccgctttttt tgataataag tttattcagg aggaaataaa atggctaaga
    38221 aaacagtgaa agatatcgat ttaaaagaca aaaaagttct tgtccgtgtt gacttcaacg
    38281 ttcctttgaa agacggcgtg atcactgacg acactcgtat caaagcagca ttgccaacaa
    38341 tcaactacgt tctagaacaa ggcggaaaag caatcctttt ctctcacctt ggacgtgtaa
    38401 aaacagaaga agataaagaa ggaaaatctt tagctccagt tgcaaaacgt ctaggcgaat
    38461 tattaggtaa agaagtaaca ttcgtacctg aaactcgcgg agaacaatta gaagaagcaa
    38521 tccgcaacat gaatgacggc gacgttgtcg tattcgaaaa tacacgattt gaagatattg
    38581 acggtaaaaa agaaagcaaa aatgatacag aactaggtaa atactgggct tcattaggcg
    38641 atgtatttgt taacgatgct ttcggtacag ctcaccgtgc acatgcttct aacgtaggta
    38701 tcgcttcaac tggtatccca acagttgctg gattcttgat ggaaaaagaa atcaagttca
    38761 tcggtgaagc agtagaagaa ccaaaacgac caatgattgc aatccttggt ggcgcaaaag
    38821 tttctgataa aatcggcgta atcgaaaact tgattcctaa agcagataaa atcttgatcg
    38881 gtggcggaat gacttacaca ttctacgaag caaaaggaat caagatcggt aactctctag
    38941 tagaagctga taaagtggaa ctagcaaaag aattaatcga aaaagcaggc gacaaacttg
    39001 tcttgccaat cgacaatgtc tgtgcaccag aattttcaaa tgatgttgaa acacaagtaa
    39061 tcgaaggaga cattcctgat ggtttgatgg ctttagacat cggcccagca tcagtaaaat
    39121 tatttgctga tacattacaa ggtgcaaaaa cagttgtttg gaacggacct atgggtgtat
    39181 ttgaaatgag taactttgct aaaggtacaa tcggtgtttg tgaagcaatc gctaacttag
    39241 aaggcgcaac aacaatcatc ggtggcggag actcagccgc agcagcagaa caattaggat
    39301 ttgctgacaa attcacacac atctcaacag gtggcggcgc aagcttggaa ttgcttgaag
    39361 gtaaagaatt accaggacta gcagcaatca acgataaata aacgaccaag tttgctcaat
    39421 tctgacgagt aatcgatcaa aaggtgaagg gagttttcat gactccctag ccatttttca
    39481 ataaattaag gagtgtgttc ctcaatgcgt aaacctatta tcgcaggtaa ctggaaaatg
    39541 aacaaaactg catcagaagc aaaagaattt gctgaagcag taaaaaacaa aatcccttca
    39601 aatgacaaag ttgattcagt catcggatct cctgcattat tcttacaaga attaatgaca
    39661 tcagctgaag gtacagaact aaaaatctca gcacaaaact gctactggga aaactcaggt
    39721 gcgttcactg gtgaaacttc accagcagct cttgctgatc taggtgttca ttatgtgatc
    39781 atcggacatt cagaacgtcg tgaatatttc catgaaacag acgaagacat caataaaaaa
    39841 gcgaaagcta tctttgcaaa tggtatgaca ccaatcctat gctgtggcga atctttagaa
    39901 acctatgaag caggcaaaac tgctgaatgg atcgaaggac aaattacagc tggtttagct
    39961 gatctttcag ctgaacaagt gtcaaacatg gttattgctt atgaaccaat ctgggcaatc
    40021 ggtactggta aatcagctga cgcacaaatc gctgacgaaa tttgtggagt tgtacgtcaa
    40081 acagttgaaa aactttatgg aaaagaagta tcagaagcag ttcgtatcca atacggcggt
    40141 tctgtaaaac cagaaaatat tgctgaatac atggcaaaag aaaatgttga cggtgcttta
    40201 gtcggtggcg caagcttgca agcagattca ttcttgtcat tgttagacgc tgtaaaataa
    40261 ttgttgaaaa tttgcgaaaa atgcaagaaa tgtttgcact aatgagcaaa attaactaaa
    40321 atcgtatatg agggaaatcc cttaggaaaa aacaaactca aaggagagac aaaacatgtc
    40381 aattattact gatgtttacg ctcgcgagat cttagactca cgtggtaacc caacaatcga
    40441 agtagaagta tacacagaaa gcggagcttt tggccgcgga atggttcctt caggtgcttc
    40501 aactggtgaa tacgaagcag ttgaattacg tgacggagac aaagctcgtt atggcggtaa
    40561 aggtgtaact aaagctgtcg acaacgtaaa caacatcatt gctgaagcaa tcattggcta
    40621 cgatgttcgt gaccaaatgg ctatcgacaa agctatgatc gctttagacg gaactcctaa
    40681 caaaggtaaa ttaggtgcga acgctatttt aggtgtttct atcgctgttg cacgtgcagc
    40741 tgctgactac ctagaagtac ctttatacca ctacctaggc ggattcaaca caaaagtatt
    40801 gccaacacca atgatgaaca tcatcaacgg cggatcacat gctgataact caatcgactt
    40861 ccaagaattc atgatcatgc ctgttggcgc tccaacattc aaagaagctc tacgtatggg
    40921 tgctgaagta ttccacgcat tagcttcaat cttgaaagct cgcggattag caacttctgt
    40981 tggtgacgaa ggtggatttg ctccaaacct tggttcaaac gaagaaggtt ttgaagtaat
    41041 catcgaagca atcgaaaaag ctggctatgt acctggtaaa gacgttgttc ttgctatgga
    41101 tgctgcttct tcagaattct acgacaaaga aaaaggtgtt tacgtactag ctgattcagg
    41161 cgaaggcgaa aaaacaactg acgaaatgat caaattctac gaagaattag tttctaaata
    41221 tccaatcatc tcaatcgaag acggattaga cgaaaacgac tgggacggat tcaagaaatt
    41281 aactgacgta ttaggcgaca aagttcaatt agttggtgac gacttgttcg taacaaacac
    41341 tcaaaaatta tctgaaggta tcgaaaaagg aattgctaac tcaatcctta tcaaagtgaa
    41401 ccaaatcggt acattaacag aaactttcga agctatcgaa atggctaaag aagctggcta
    41461 cacagcagtt gtatctcacc gttctggtga aacagaagat tcaacaatct ctgatatcgc
    41521 tgttgcaaca aacgctggcc aaatcaaaac tggttctcta tcacgtactg accgtattgc
    41581 taaatacaac caattactac gtatcgaaga ccaacttggt gaagttgcag aatacaaagg
    41641 tttgaaatct ttctacaact taaaaaacaa ataattcttt tctaatgaag atacatgatc
    41701 gactattgaa agatagtcga gtatgatcac ttagttaagt aatcgaactc tctacttgct
    41761 gaaatatcag caggtagaga gttttttttg caactagctt ctgtatatga tgtttacaaa
    41821 gaatggtaca agaaaagatc atccttgaaa aaggaaagct aacaataact agcatcatat
    41881 aagagctttt aaggatagaa cagtagaata ggaatctatg agcaagtcct ctcgtataaa
    41941 taaaatagga gggaagtagc agcatatttt tcataaagtg gctctctttc ttcattttta
    42001 ctttggttgc tcctatttga aaattcgagc acactcaaat acaataaaaa gaaatggcaa
    42061 gggagaggat tagatgaaaa aagaagaaaa aatcgcgatt ttacaagaga tcatccgaat
    42121 taaatcagtg aatggaaacg aaggtgaagt agcagcctat ttgaataagc ttttggcaaa
    42181 gcatgacata acaggagaga ttgtttctta cagagatggt agagataatt tgattgctcg
    42241 ttaccaaaag ggccaatcag gaaaagtgtt aggcctatct gggcatatgg atgtcgtggc
    42301 ggctggtgat gaatcgtctt ggacctatgc accctttgca gcagaaatcc atgggaaccg
    42361 tttgtacgga cgtggtgcta cagatatgaa gagtggcttg gcagcaatgg tgattgccat
    42421 gatcgaactg aaagagtcag gaaaaccatt taacggaaca gtcaaattat tagccactgt
    42481 cggagaagaa gtgggtgaac taggtgggga acagctcacg aaagcaggct atgtagatga
    42541 cttagatgcc ttgatcatcg gagagccaac taattacagt ttgatgtata cgcacatggg
    42601 atcaatcaac tatacagtca cctcgcacgg aaaagaagcg catagctcta tgccagacca
    42661 aggttataat gcaatcaatc acctaaatga attcattacg aaagcaaatg ctgaaatgaa
    42721 tcacctcgct gaaacaatcg agaatcctgt cctaggaaaa acaatccata atgttacatt
    42781 gatcagtggc gggaatcaag tcaacagcat cccaagccat gcgcagctcc aagggaacat
    42841 tcgatcgatt ccagaatacc caaatgataa aatcattgca ttgctgcaat caatcgtcaa
    42901 tgaattgaat caagagacag actatcattt ggaattgatg atcgattaca acaaaatacc
    42961 tgtcaaagct gatccagatt ctcctttgat tcattgcatt cagcaacaat tcagccagcc
    43021 attaccttta gttggagcgg cagcaacaac ggatgcagcc gaatttacga aagctaacca
    43081 ttccttcgac tttgtcgtct ttgggcctgg tgtcgttact ttgcctcatc aagtagatga
    43141 gtatgttgaa atcgataatt atttggatat gattgaaaaa tatcaaggaa ttattttatc
    43201 ctacttagcg taagattttt ttaataaatg aaaaaacaga gccagattag cattattttg
    43261 ctaatccagc tctgtttatt cagcctattt ttctttttga atatatcaag cgttgtggca
    43321 acatagtgat gactaatgaa acaacgacga tagacaacaa acgatcaagg tagtctgtac
    43381 ctgcctgtac caagatcaca cttgttgtct gacttaaacc taacccatgt aatagctgaa
    43441 cgatcatact ggatcctgag gaggtgatcc cgccaaataa ctgaacggta atgatcgaag
    43501 aaacgatcgt tccaggtacc gacaaaatca aagcgagtaa taacagtttc aagctgtttt
    43561 tatctttgcc gagatatctt ggcaaaatca atccagcaag caagcctgtg agaatttgga
    43621 ttggcgaata atacagagaa aaaatatctg tagtcactcc acttaaaata ccgctggaca
    43681 atcctgtcag cattccaggt acaggcccaa gcaatgcacc agcaaaaatc gttccgatag
    43741 aatcgagata gattggcaaa cgaagccata aagcaatcgt tcctccaata taattgatcc
    43801 caatacacaa agccatcact gtaagtacgc gagttgccat tttgttttta ttcattttgt
    43861 tagcccatct ttaacctttc caaatctttc ataatcgttt cttcttgtgc atttaaaaga
    43921 acggttaaaa actttcggaa aaaaagacga gtatctactt ttgtcataat tttactgtta
    43981 gcaggtttct gccaaaattc ataacgatca accaatgttt gtccacgact tattccttga
    44041 gtttccacag cagtgaagct tttgaaacct tcacagatgg ccccctcgat aaaataagca
    44101 acagctaacg gatcattgat gacacatcct aaaattcttt cttgtttcca gtggaagtca
    44161 aaataaaaac gagtgatcgc ttttagatac tctccctctt caggattcat ttgacagcag
    44221 tattcgagga tagtcggtgt caagacgatt tctcttgtca catctaaccc aaccatttct
    44281 atcgtttgtt tgagattctc gaatacataa gaagctgctt caggatcaca ccagtaattg
    44341 tattcagcaa ctggcgaaca gttaccatgg cttttgtaag ttccacccat cgagacaaaa
    44401 cgatccatat gtttcccaat atttgggttg actttcaaag caaaagcgat attggtcaaa
    44461 ggacccaaag caatgactga tgtatttgtc ttttctttga aataatcagc caaaaaatca
    44521 acggcatgga gaggttctgc ctgttttctc aagatcatcg ggaaatttgt ttcccctaaa
    44581 ccatccatac catgagtgtc ttgtgcactg atgaaaggtt gcttcaaagg tttttccatt
    44641 ccttgataaa caggaatgtc taaacgatca cagcgttcca ggcatttcag cacattctgc
    44701 gtacctatat ctgcagggac attcccacaa actacggtaa tagcaactac ttcgatatca
    44761 ggggatttga cagctaatga taaagctaaa gtgtcgtcga tccctggatc gcaatcaata
    44821 attacttttc gcattatttc caatctcctt tctttagaga tacttagttt tttattctgg
    44881 gaagtttccg aaccagtcca agacgagcgt cttgattata atgcacgaaa ttagtatact
    44941 ggcttttatt gcgaatgtaa atgctctctt acaagtaaat ctattgagga gaactttaag
    45001 taaatgatac aatgaaaaag aaatggaatt tacgtgtgcc gaaaataaag aatcctatgg
    45061 atcggtacat tggataatag ttagattata gtttggcaga aagcggcggg tgtacaatgg
    45121 ttatttttta tttcattctt attctgataa ttatagggat cagttaccat tttctttctt
    45181 atttttatgc aaagaaatta gtaagagcgg gagtacagag aggatatgac tggtatgaag
    45241 acactggtca tcgtttgatg gaccctgctg cagtcacgtc agtagaaaaa aaacgacaaa
    45301 aattagaaga agagtgtgaa agcttttggg agcagggaac acccaaaaca atcaaaagtt
    45361 atgacgggtt gaaactagct ggtcagatgt tcatccatcc aaataagcag aataaatggg
    45421 tcatttgcgt acatgattac cgaagcacag ggaaaagaga catgtctcat ataggtaaaa
    45481 gatatgcaga gaaaggattc aacgttcttg tacctgattt acgtgcgcac ggagaaagtg
    45541 aaggagaaat catcggaatg ggatggctag atcggctaga tttaattgcc tggatccaac
    45601 tcatcttaga tgaacagccg aatgcgtcca ttattttaca cggcggatca atgggagctt
    45661 ctacgatcat gatggcaagt ggtgaaaaac ttccatcagc ggtcaaagga tttattttag
    45721 acagcggata tgtttctgtt tatgcagaat tccgatatat gttaagtaaa atcactgtct
    45781 ttcctaaaaa aatggtcatg agatatgcga atcattatgc ccagaaatac gccggttact
    45841 ccttaaagca agcatcagct acgagacagc ttggcagcaa ccatctgcct ttattggtca
    45901 ttcatggtga aagagatcat tttgtcccta cagaagcagc gtacacgatt caaaatgcta
    45961 cggcagggga taaagcactg ctgcttgttc cagaagcaga acacttggaa gcatcgatga
    46021 aagatccgaa aacctattgg acagtgattt tttcatttat tgaacaaaga ataacgatga
    46081 attaattttc tcttcttttt tgtaagtaat tcgaggataa ataattgttt atagatcagg
    46141 agactgctaa aataaggtag gaataaaaaa atatcagtta tttgaggagt gcggcatatg
    46201 gaaatcaaag aagaaaaaaa tcgtcttgct ttattcaatg atgaacaaca agagatcgga
    46261 gaaatgacat ggtcagatgc gggcccagac atcatgatca ttgaccatac atttgtagat
    46321 ccagcatatc gtggtcaaaa gttagctgaa aagctagtgt atactggagt agaattagca
    46381 agacgagacg ggaaaaagat cattccttta tgcccttatg caaaaaaaga gtttgaaaag
    46441 aaaccagaat atcaagacgt cttacgtcag tcataagaat aacagctgaa agttttgaac
    46501 tttcagctgt tattccattt atatagagtt gtcacggact tttatctatg ataaagttaa
    46561 agagacgata tcttttggga aggaggctgc gatatgtata atttaatttt gggaatcgtg
    46621 atcgttttat cgattgttat ggtcattgcg attatgatgc agccaagcaa gcaaaatagt
    46681 gcagcgagtg catttaccgg cggtgcggat caactattcg gcaaacaaaa agctcgcggc
    46741 tttgaagctg tcatgcagcg atcaactgca gttatgggag ctatctggat gatcttatta
    46801 tttgtcctag catatttatc ttcgaagtag ttgaataagc ctctcgcaaa acggcgggcg
    46861 gcttttttta ttgcaaagag aacgcttaag aagaagttat gaaagaaact atggtaaaat
    46921 gaggcgaaag aggtgagacg agtgagacaa ttaccaaaac ccctttatgc aaaacatgga
    46981 aagcgagcgg ttttgctgct tcacgcttac tcaggaagtc caaacgatgt tcgaatgctc
    47041 gcacgatttt tggaaaaagc agattataca gtttatgcgc cgctttttaa aggacacgga
    47101 acaatggagc catatgatat tttgcaagaa aaagcagaaa gctggtgggc agacactaaa
    47161 aaagccatcc attttttaca gtctgagcag ttttctgata ttgccgtatt aggattatca
    47221 atgggcggca tctttgctgt acgagcacta gaagaagaat cagtgattgg cggcggtttt
    47281 ttctgttcgc ctttgtctcc tgtaaaaaca aacgtgcctg aaaactttga aaaatacgta
    47341 cgtcaagtgc tgaagacagc aggtaaatca gagaaagaaa tcaatgaaaa agcagttgct
    47401 taccgctcgt tagctgaaca acaattaatg gatatacaag atcaggcagc aatcgtagaa
    47461 agtaggttgt cagatatcca acagccgata tttttagctc aagctgggaa agatgaaatg
    47521 atcgacccta atggcgtatt tgaaactgca cgcaagttga gccgtcagcg agtgacactt
    47581 cgatggtatc ctgaaagtgg tcatgtgatt acagttggta cagcaagacg cgaattagaa
    47641 aaagacgtct tggaattttt agaaaaatta ccttggaatg aggaataaat atgacaaaag
    47701 aaacgataaa agagaaaatt ctctttttca tggaaagcag tcgcaaacgc agtttttcta
    47761 tggaagaaat cgctgaaggt ttagggttcc aaaaaagcga tgattttaaa ttactagtac
    47821 aaacgattgc tcaaatggaa agagaacaga gtatagtttt caataaaaaa ggaaaagtga
    47881 aacttccttt aaaaccaatc ttgatcgaag gaacatttcg agcaaatgaa cgaggatttg
    47941 gatttgtaac gattgatccc gaagaagatg acatctatat cccaaaagaa gcgactggtt
    48001 ttgcaatgga tggagacacc gtagcaatcg atatcatgaa gcgagcaaat acagatatgg
    48061 atcgcggagc agaggggaaa gtagtcgaga ttcgagaccg ggcgactact cagttagcag
    48121 gggaattcgt tgcttattca gaagaagaaa tacaagaaac ggatctttac ggcgttgtta
    48181 tcccgaaaga tagaaagctc aaccaattta aagtatatgc agcagcagag ggagttcgtc
    48241 cagttgacgg aagtattgtg ctgatcgaac tgacccatta ccctgaaaaa gggtatgcga
    48301 ctagtttaga agggataatc aagcaagtga ttggtcataa gaatgaccct ggcatggata
    48361 tcctctcaat tgtcgtggct aatggtatcc ctacgaaatt tcctgatgat gtattgtctg
    48421 aagcagatga agttccagat caaatcagcg aaaaggatat aactggacgt agagatttaa
    48481 gaaatcagct cattgttacg attgatggag aagatgcaaa agatctagat gatgcagtta
    48541 cggttaaaaa attagataac ggtaattatt ttcttggagt acacattgca gatgtttctt
    48601 actatgtgac acaaggcagt caattggaca gagaagcata tgaaagaggg acaagtgtat
    48661 atttgactga tcgagtgatt ccgatgatcc cgcaacgttt atcgaatggt atctgttcac
    48721 tgaatcccca tgttccaagg ctgaccatga gttgcgaaat ggagattgat ccaaatggac
    48781 aagtagtctc tcatgatatt ttcccaagtg tgatccaaac aacagaacgg atgacctaca
    48841 cagctgtcaa tcaaatctta gaagatcaag acgaagaaac gatgcaacgc tatgctcagc
    48901 tcgtacctat gttccaagaa atgggtgaat tgcatcgaat ccttgaaacg atgcgaatca
    48961 aacgtggcgc gatttctttt gaggatcgcg aagcaaaaat ccttgttgat gctgaaggac
    49021 acccaaatga tatccagcta agaagcagag gaattggtga acgcttgatc gaatctttca
    49081 tgcttgcggc caatgaaacg gtagctgaac attataataa attaaatctg ccgtttattt
    49141 accgaatcca tgaacagcca aaagaagaaa aaatgcagcg cttctttgat tttgcttcag
    49201 ctttgggcat tctggtccga ggaacgaaag gaacgatcac accaaaagat ttacagcatg
    49261 tgatcgaagg agtagaagac aaaccagaag ctgcagttat caatactatg ttgctaagaa
    49321 gcatgcaaca agcacgctat tcagaagaca attttgggca ttatggctta gcggcagatt
    49381 actacacaca ctttacatct cctattcgac gctatcccga tctaatcgtc catcgtctga
    49441 tccgttctta cagtgaagat aaaagtgaaa gcaatcaaag ttcgtgggct acggaacttc
    49501 ctgaaattgc agatcatagt tctaagatgg aacgtcgtgc agtagaaaca gaacgtgaag
    49561 tagattccat gaagaaagca gaattcatga tggacaaagt gggggaagaa ttcgatggga
    49621 tcatcagctc tgttgtgaaa ttcggcctgt ttatcgaact gccgaataca gtagagggat
    49681 tgatccatat caatgaattg aaacaggact acttccattt tgttgaaaat catttagctc
    49741 ttgtaggtga aagaactggt ctgactttta aaatcggaca gaaagtgcga gtaaaagtag
    49801 tcaaagctga tccagatgaa cgagcagtag attttgaact tgtttccgca gaagaggtta
    49861 cagcgttgga acggccaaaa acacgaaatg gaaaatcagg aaaacgcgaa ggaaaacaag
    49921 caggaaaacg cccagaaaga aatggcaaga agaacaaacg atatagcgat agtaagcaaa
    49981 cgttcggtaa gaaaaaaggg aagaagccat tttacaaagg tgttaaaaag aagaaaaaga
    50041 aaaaataaaa tgagttggag gaaacagcta tgccaaaagg cgagggaaaa ttaattgcac
    50101 aaaacaagaa agctcgccat gattattcga tcatcgacac gatggaagca gggatggtct
    50161 tgcaaggaac cgagatcaag tcgatacgaa acagccggat caatctaaaa gatggattta
    50221 ttcgcgtccg caacggagaa gctttcttgc ataatgttca tatcagtcct tatgaacaag
    50281 gaaatatttt taatcatgat ccgttgcgca cgagaaagtt attattacac aaaaaacaaa
    50341 tcatccggct tgaaaatgaa ttgaaaaata ctggaatcac tgttgttcct ttaaaagtct
    50401 atattcgtaa cggctatgcc aaggtattga ttggtctggc gaaagggaaa aaatcttatg
    50461 ataaacggga agatttgaaa cgaaaagata tcgatcgaca aattgatcga acattaaaaa
    50521 atttctctag ataatttcat tatccttttt cgcatattcg gtgcttttgc ttttacttaa
    50581 tgataaaatt tgttagcatt taatctgata aaacatgatt atcataggtt ttttcttaga
    50641 tcaggagcac cgtgtataaa gaaaaattcg cttcgttttt gactgaaata aaaaagtgtc
    50701 taattttcat gaaaatgttt tgaattttct gtttcatggt ggtatgatac cgtgtggtta
    50761 gaaaccgatt ggaaaaaacc agatacgcac atgaattgat tttatgtgaa tttcggggaa
    50821 aagaggtgaa atggattgga tgaacgctcg ctaacgttcc acattggacc aatttggttt
    50881 gatggtacag tttgtataat ggtgttactg acctgtttga ttgttttctt cttagcttac
    50941 ttttttacaa gaaatcttaa gttgaaacca acaggcaaac aaaatgcgat agaatgggtc
    51001 attgatttta gtcgaggaat cgtaacagat aatctgccta gaaaagaatt gagcaatttc
    51061 catttgttgg catttacatt gtttttattt gtttttgttg caaacaacat cggtttagta
    51121 acaaaaatcg ttttacctaa cgaaacaaca ctttggaaaa gcccgacagc agacccgttt
    51181 gttactttaa ctttggcatt tataatgatc ttacttactc acttatttgg ggtgaaaaag
    51241 ttaggcttca aaggatattt cgtaaattcg ttcttgaaac catatagttt tatgtttccg
    51301 atgaaactga ttgaagaatt tacaaactta ttaacacttg ctttacgtct ttatgggaat
    51361 atttatgcgg gggaagtttt gctaaccttg attgcaaaaa tggtaataag cttaggctgg
    51421 ttctcattgc cgttagcaat tccattagag atggtctgga tcgcattctc cttgtttatc
    51481 ggaagcatcc aagcatttgt cttcgttact ttatctatgg tttatatgag ccataagatt
    51541 gaagtggaag aataatattc gttttaaaaa caaactacta tttatctaat ttaggaggaa
    51601 aacaattatg aattatatcg cagcagcaat cgcaatcatg ggagcagcaa tcggagccgg
    51661 ttatggtaac ggtcaagtta tctctaaaac aatcgaatca atggctcgcc aacctgaaat
    51721 gtctggtcaa ttccgtacaa caatgtttat cggggtagct ctagtcgaag ctgttcctat
    51781 cttaggggta gttgtcgcct tgattctagt ttttggtgtt taattgaatc agtattaagg
    51841 cagaaacaat ttggtttgtt actgcttttt tacatgaaaa ctagaagtac ttcaaagaaa
    51901 ggaaggctcg cctatgctga atcaattggc aattgcagaa gttggcaatg cgatgttagg
    51961 aaatgtcatc gttgtcagcg gctcattttt gatactactg gctctcttga agcattttgc
    52021 ttggggacca atcagcgata ttttgaaaaa acgtgaagac aagatcgcca atgatttgga
    52081 ttctgcagaa caatctcgca tcaactcagc gaaaatggaa caagaacgcg aacaacaatt
    52141 gttagcctct cgttctgatg cagctgatat catcaaaaat gcgaaagaaa gtggagaatt
    52201 aagccgccaa aatattttga aggatgctca agaagaagca gctcgtctaa aaagtaaagc
    52261 ccaagctgat atcactgtag aacgtgattc agcgctgaac tctgtaaaag acgatgttgc
    52321 agaactatct cttcaaatcg cggaaaaaat cttgaacaaa gaattatctc cagaaatgca
    52381 cgaatcatta atcaatcaat atatcgaagg tctaggttct tcaaatgaaa ctagataaat
    52441 atacagtagg aagacgttac gggaaagctt tatttgaact ggcgatcgat tcaaataaag
    52501 cagaagaaat ttatcaagat ctcgtaagct tacgtcaaat ctatgaacaa gtacctggat
    52561 taggtaatat gttaagtgat gttcgtctag aacctcatga aaaacgagaa atcatggaca
    52621 agcttgttag tggctatgat ggaatcgtta aaaacttttt agaagttgtt tatgcgtata
    52681 accgaatgga tgatcttttg ttcatgatcg aagaatatga acgtcgatat gatgaacatc
    52741 aaggtttgct gttgggaagt gtcacaacag cagttccact ttcagacgaa caacgaatac
    52801 aattagaaaa gaatgttgca aaaacaatga actatcaatc agtcgaatta aaacaaatcg
    52861 ttgattcttc tattattggt ggcgcgattg ttgaagctaa ccatcgagta atcgacggaa
    52921 gcattcgcac gcaattagaa aatatgcgta atcaattaaa tagatagtac aaagaggtag
    52981 gtgaatcgaa tggccatcaa agcagaagaa attagtgcct tgattaaaga acaaattgaa
    53041 aattatcaaa acgtgctttc agtcgaagaa atcggtaccg taacttatgt aggtgacggg
    53101 atcgctcgtg cacatggttt agaaaacgcg atgagtggtg agttgcttga attttcaaat
    53161 ggctcatacg gaatggcgca aaacttagaa tcaaatgatg taggtatcat tatccttggc
    53221 gatttcgagt ccattcgtga aggagataaa gtaaaacgaa caggaaagat catggaagtt
    53281 ccagttggag aggccttgat tggacgggta gttaatccgc taggtcagcc aattgacgga
    53341 ctaggtgaaa tcgttactga caaagctcgt ccagtagaag caatggcacc aggcgttatg
    53401 caacgtaaat ctgttaacga accaatgcaa acaggtctaa aagcaatcga cgctcttgta
    53461 ccaatcggac gcggccaacg tgaattagtt atcggtgacc gtaaaacagg gaaaacttcc
    53521 attgcgatcg atacgatcat caaccaaaaa ggtcaagata tgatctgtat ctatgtagca
    53581 atcggacaaa aagattctac agttcgtaca caagttgaaa cattgaaaaa atatggcgca
    53641 atggattaca caatcgttgt gaatgccggt gcgtctcaac cagcgccgtt gctttatatc
    53701 gcaccatatg ctggtactgc aatgggtgaa gagttcatgt acaacgggaa acatgtcttg
    53761 atcatttttg atgatctttc aaaacaagct gttgcttatc gtgaactttc cttgctgctt
    53821 cgccgtccac caggtcgtga agcttatcca ggggatgttt tctacttaca ctcacgctta
    53881 ttggaacgtg cagcaaaatt aagtgatgat ttaggtggcg gttcaatgac tgccttacca
    53941 tttgttgaaa cacaagctgg agatatctct gcttatatcc caacaaacgt tatttcgatc
    54001 actgatggac aaatcttctt agaaagcgac ttgttctacg caggtactcg tccagcggtt
    54061 gatgccggac tatctgtatc tcgtgtaggg ggttctgcgc aaatcaaagc aatgaaaaaa
    54121 gttgctggga ctttacgtct agacttagca agttaccgtg aattagaagc atttacacaa
    54181 ttcggttctg acttggatgc agcaacacaa gcaaaactaa atcgtggacg ccgtacagta
    54241 gaaatcttga aacaaaaatt gcatgcaccg ctagctgttg aaaaacaagt agtgattttg
    54301 tatgctttga cacatggatt cttagatagt atccctgtag acagtatttt agacttcgaa
    54361 catgaattat tcgaatatct ggataccaat cattcagata tctttgaaac aatccgtaca
    54421 acaaaagatc ttccagaaga agaagcgttg aacagtgcga ttcaggaata taaagacatg
    54481 ttcttagcta caaaaggaaa taattcctct acagaagata aattgaaatc cattcaaaat
    54541 gcatagtgag gtgagatgaa tgggtgcttc attaaatgaa atcaagacac gtatcgcttc
    54601 aacgaaaaaa acgagccaaa tcacgcgggc aatgcagatg gtgtctgctt ctaagttgac
    54661 aaaatctgaa gcttcttcac agaagtttca ggtttatgca acaaaagtaa gagaaatcgt
    54721 tacgcatttg actgctactc agttaaatga tattgcctca gaaaatccta gaggggatat
    54781 caattacaac agcatgttgc tcagccgtcc tgttaagaaa acgggttata tcgtcatcac
    54841 tgcagatggc ggtttagtcg gtggatataa cagctcgata ttgaaacaaa cgatgtctat
    54901 tttagaagaa gaccaccaat cacccgatga ttacgtgatg atcgcaattg gcggaactgg
    54961 tgcagacttc tttaaagcac gcggtatcaa tctagcatat gagctgcgca atctatctga
    55021 tcagccaagc tttgatgaag tccgcaaaat cgtaaatatg gcaacaacta tgtaccaaaa
    55081 cgaagtattc gatgaattat acgtatgtta taaccatcat atcaattcgc taactagtca
    55141 gtttcgtgta gaaaaaatgc tgccaatctc agacttagat ccagaagaag caactacttt
    55201 cgaacaagaa tatatttttg aaccttcaaa agaagaaatc ttgtctcagt tattgcctca
    55261 gtatgctgaa agtttgatct acggtgcgat cgttgacgcc aaaacagcag aacatgctgc
    55321 tggtatgaca gcaatgaaaa cagcaactga taatgctgct aatatcattg atgagctgac
    55381 ggtttcttac aaccgtgcaa gacaaggggc tattacccaa gaaattaccg aaattgttgc
    55441 cggagcttcg gcactagaat agtattgaga ttggaggaaa aaagatgagt tcaggcaaga
    55501 ttgttcaagt aatcggtccc gttgtcgacg tggaattttc tttagaccaa tccttaccag
    55561 atatcaacaa cgcattagtt gtttataaaa atgacgaaaa taaatcaaaa gttgttcttg
    55621 aagcagcgtt agaattaggg gacggagtga tccggaccat tgcgatggaa tcaacagatg
    55681 gtttacaaag aggaatggaa gtcattgata caggcaaagc aatctctgtt cctgtaggta
    55741 aagaaacatt aggtcgcgta ttcaacgtac taggagatac gatcgattta gaaacacctt
    55801 tcccagaaga tgcggaaaga agcgaaattc ataaaaaagc accagccttt gacgaattaa
    55861 gtacaagtac agaaattttg gaaacaggga tcaaagttat cgatttgctt gccccatatt
    55921 taaaaggtgg gaaagtcgga ctattcggtg gtgccggtgt tggtaaaacc gtactgatcc
    55981 aagaactgat ccataatatc gcccaagaac atggtggtat ttctgtattt accggtgtag
    56041 gtgaacgtac tcgtgaaggt aatgacttgt attatgaaat gaaagattca ggagttatcg
    56101 aaaaaacagc catggtgttc ggacaaatga acgaaccacc aggtgcacgt atgcgtgttg
    56161 ctttgactgg tttgacgatt gcggaatatt tccgtgatgt agaaggtcaa gatgtactgt
    56221 tgtttatcga caacattttc cgtttcactc aagctggatc tgaagtatca gccttgttag
    56281 gacggatgcc ttctgcggtt ggttatcaac caacattggc aacagaaatg ggtcaattgc
    56341 aagaacgtat cacatctacg aaaaaaggtt cgatcacatc gatccaagca atctatgtgc
    56401 cagccgatga ctacactgac ccggcgccgg caacagcatt cgcccatttg gatgcgacaa
    56461 ccaacttgga acgtaaatta acagaacaag ggatttatcc agcggtagat ccattggctt
    56521 cttcttcaag tgctttagca cctgaaatcg tgggagaaga acattataaa gtagcaacag
    56581 aagtccaaca cgtattgcaa cgatatcgtg aattacaaga tatcattgct atcttaggta
    56641 tggatgaatt atccgatcaa gaaaaagtgt tagtttctcg tgcccgcaga gtccaattct
    56701 tcttgtcaca aaactttaac gtggctgaac aatttactgg tcttccaggt tcatatgtcc
    56761 ctgtagaaga aacagtgaaa ggattccgcg aaatcttgga aggcaaatac gatgatcttc
    56821 ctgaagaagc tttccgaagt gttggccgaa tcgaagacgt cgtagaaaaa gcgaaaacac
    56881 taggctacta gaaaggggtg ccccatggat caatatttaa cagtaaatgt ggtaactcct
    56941 gccggtttgg tctatgatca ccatgcagct atcgtcgttg cacgtacgac agctggtgag
    57001 cttgggattt tgcctaaaca tgcaccaatc atcgttccat tgacgattga tgaagttcgt
    57061 gtaaaacgaa cagattcaga tacccacgtc gattggatcg ctgttaatgg cggaatcatg
    57121 gaagttcgtg ataatgttgt ttcaatcgtt gctgatagtg cggaacgtga acgtgatatc
    57181 gatgtatctc gtgcggaacg tgcaaaacaa cgcgcagaac gccaaatcga agaagcaaaa
    57241 gcaaaacaag atacgaacga attgaaacgg gcaactgttg cgttgcaccg agcaatcaat
    57301 cgtatcaaag tatctaagca ctcataaaat gagatgcgga tgtttatccg tgtttaagaa
    57361 cagtaaaaag aggttatgac agaagtggtc agcttcaaga cataagaagg aatttacgaa
    57421 aattgcttct caaatttttg tgaaatctca gcttattttt cgaagaagct acttctggaa
    57481 caccgtatat cggaggttgt gaaatcagcg ttttgtcctg agtattagag aaacaaatgg
    57541 aaaatagttc ctcatatttt gccgtttttt gagctaatac cgagggtcag atgattgaac
    57601 accgtttatt tggaataagg gactgcgaca agacttt
//
LOCUS       DACEUZ010000003        57198 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.003, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000003 DACEUZ010000000
VERSION     DACEUZ010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 57198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57198
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.003"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            <1..120
                     /locus_tag="IXG44_000146"
     CDS             <1..120
                     /locus_tag="IXG44_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HAQ1656891.1"
                     /translation="YLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            complement(204..926)
                     /locus_tag="IXG44_000147"
     CDS             complement(204..926)
                     /locus_tag="IXG44_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAQ1656892.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(916..1653)
                     /locus_tag="IXG44_000148"
     CDS             complement(916..1653)
                     /locus_tag="IXG44_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1656893.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            complement(1665..2657)
                     /locus_tag="IXG44_000149"
     CDS             complement(1665..2657)
                     /locus_tag="IXG44_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1656894.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            complement(2672..3415)
                     /locus_tag="IXG44_000150"
     CDS             complement(2672..3415)
                     /locus_tag="IXG44_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1656895.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     regulatory      complement(3489..3593)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(3656..4978)
                     /locus_tag="IXG44_000151"
     CDS             complement(3656..4978)
                     /locus_tag="IXG44_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ1656896.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     gene            complement(4965..5930)
                     /locus_tag="IXG44_000152"
     CDS             complement(4965..5930)
                     /locus_tag="IXG44_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1656897.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            complement(5927..7465)
                     /locus_tag="IXG44_000153"
     CDS             complement(5927..7465)
                     /locus_tag="IXG44_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HAQ1656898.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            complement(7881..8168)
                     /locus_tag="IXG44_000154"
     CDS             complement(7881..8168)
                     /locus_tag="IXG44_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1656899.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            8302..8970
                     /locus_tag="IXG44_000155"
     CDS             8302..8970
                     /locus_tag="IXG44_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ1656900.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            complement(9261..10001)
                     /locus_tag="IXG44_000156"
     CDS             complement(9261..10001)
                     /locus_tag="IXG44_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1656901.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(10131..11078)
                     /locus_tag="IXG44_000157"
     CDS             complement(10131..11078)
                     /locus_tag="IXG44_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1656902.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            complement(11207..11971)
                     /locus_tag="IXG44_000158"
     CDS             complement(11207..11971)
                     /locus_tag="IXG44_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ1656903.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            complement(11975..12364)
                     /locus_tag="IXG44_000159"
     CDS             complement(11975..12364)
                     /locus_tag="IXG44_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1656904.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            12499..13041
                     /locus_tag="IXG44_000160"
     CDS             12499..13041
                     /locus_tag="IXG44_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HAQ1656905.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            13189..13821
                     /locus_tag="IXG44_000161"
     CDS             13189..13821
                     /locus_tag="IXG44_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1656906.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(13955..14080)
                     /locus_tag="IXG44_000162"
     CDS             complement(13955..14080)
                     /locus_tag="IXG44_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1656907.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(14214..15107)
                     /locus_tag="IXG44_000163"
     CDS             complement(14214..15107)
                     /locus_tag="IXG44_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HAQ1656908.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            complement(15289..16215)
                     /locus_tag="IXG44_000164"
     CDS             complement(15289..16215)
                     /locus_tag="IXG44_000164"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HAQ1656909.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            complement(16300..17061)
                     /gene="pflA"
                     /locus_tag="IXG44_000165"
     CDS             complement(16300..17061)
                     /gene="pflA"
                     /locus_tag="IXG44_000165"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HAQ1656910.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            complement(17305..19536)
                     /gene="pflB"
                     /locus_tag="IXG44_000166"
     CDS             complement(17305..19536)
                     /gene="pflB"
                     /locus_tag="IXG44_000166"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAQ1656911.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            complement(19813..22263)
                     /gene="parC"
                     /locus_tag="IXG44_000167"
     CDS             complement(19813..22263)
                     /gene="parC"
                     /locus_tag="IXG44_000167"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HAQ1656912.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            complement(22275..24320)
                     /gene="parE"
                     /locus_tag="IXG44_000168"
     CDS             complement(22275..24320)
                     /gene="parE"
                     /locus_tag="IXG44_000168"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HAQ1656913.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            complement(24486..24920)
                     /locus_tag="IXG44_000169"
     CDS             complement(24486..24920)
                     /locus_tag="IXG44_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HAQ1656914.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            25047..25685
                     /gene="plsY"
                     /locus_tag="IXG44_000170"
     CDS             25047..25685
                     /gene="plsY"
                     /locus_tag="IXG44_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HAQ1656915.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            complement(25817..26692)
                     /locus_tag="IXG44_000171"
     CDS             complement(25817..26692)
                     /locus_tag="IXG44_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAQ1656916.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(26774..27565)
                     /gene="codY"
                     /locus_tag="IXG44_000172"
     CDS             complement(26774..27565)
                     /gene="codY"
                     /locus_tag="IXG44_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HAQ1656917.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(27583..28983)
                     /gene="hslU"
                     /locus_tag="IXG44_000173"
     CDS             complement(27583..28983)
                     /gene="hslU"
                     /locus_tag="IXG44_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HAQ1656918.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(28997..29545)
                     /gene="hslV"
                     /locus_tag="IXG44_000174"
     CDS             complement(28997..29545)
                     /gene="hslV"
                     /locus_tag="IXG44_000174"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HAQ1656919.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(29582..30487)
                     /gene="xerC"
                     /locus_tag="IXG44_000175"
     CDS             complement(29582..30487)
                     /gene="xerC"
                     /locus_tag="IXG44_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HAQ1656920.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(30685..32061)
                     /gene="trmFO"
                     /locus_tag="IXG44_000176"
     CDS             complement(30685..32061)
                     /gene="trmFO"
                     /locus_tag="IXG44_000176"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HAQ1656921.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(32030..34108)
                     /gene="topA"
                     /locus_tag="IXG44_000177"
     CDS             complement(32030..34108)
                     /gene="topA"
                     /locus_tag="IXG44_000177"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HAQ1656922.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(34230..35087)
                     /gene="dprA"
                     /locus_tag="IXG44_000178"
     CDS             complement(34230..35087)
                     /gene="dprA"
                     /locus_tag="IXG44_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HAQ1656923.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(35143..35910)
                     /locus_tag="IXG44_000179"
     CDS             complement(35143..35910)
                     /locus_tag="IXG44_000179"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HAQ1656924.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(35903..36763)
                     /gene="ylqF"
                     /locus_tag="IXG44_000180"
     CDS             complement(35903..36763)
                     /gene="ylqF"
                     /locus_tag="IXG44_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HAQ1656925.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            37023..37421
                     /locus_tag="IXG44_000181"
     CDS             37023..37421
                     /locus_tag="IXG44_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAQ1656926.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            37658..38200
                     /locus_tag="IXG44_000182"
     CDS             37658..38200
                     /locus_tag="IXG44_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAQ1656927.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(38283..38834)
                     /gene="lepB"
                     /locus_tag="IXG44_000183"
     CDS             complement(38283..38834)
                     /gene="lepB"
                     /locus_tag="IXG44_000183"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAQ1656928.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(38986..39213)
                     /locus_tag="IXG44_000184"
     CDS             complement(38986..39213)
                     /locus_tag="IXG44_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HAQ1656929.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            complement(39210..39728)
                     /gene="msrA"
                     /locus_tag="IXG44_000185"
     CDS             complement(39210..39728)
                     /gene="msrA"
                     /locus_tag="IXG44_000185"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ1656930.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            complement(39748..40461)
                     /locus_tag="IXG44_000186"
     CDS             complement(39748..40461)
                     /locus_tag="IXG44_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAQ1656931.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            complement(40464..41360)
                     /locus_tag="IXG44_000187"
     CDS             complement(40464..41360)
                     /locus_tag="IXG44_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAQ1656932.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            complement(41518..42360)
                     /locus_tag="IXG44_000188"
     CDS             complement(41518..42360)
                     /locus_tag="IXG44_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ1656933.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            42492..43145
                     /locus_tag="IXG44_000189"
     CDS             42492..43145
                     /locus_tag="IXG44_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAQ1656934.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            complement(43208..43735)
                     /locus_tag="IXG44_000190"
     CDS             complement(43208..43735)
                     /locus_tag="IXG44_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ1656935.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(43755..44702)
                     /locus_tag="IXG44_000191"
     CDS             complement(43755..44702)
                     /locus_tag="IXG44_000191"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAQ1656936.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            complement(44714..46600)
                     /locus_tag="IXG44_000192"
     CDS             complement(44714..46600)
                     /locus_tag="IXG44_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ1656937.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            complement(46760..48838)
                     /locus_tag="IXG44_000193"
     CDS             complement(46760..48838)
                     /locus_tag="IXG44_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAQ1656938.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            complement(48851..49126)
                     /locus_tag="IXG44_000194"
     CDS             complement(48851..49126)
                     /locus_tag="IXG44_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1656939.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            complement(49488..49943)
                     /locus_tag="IXG44_000195"
     CDS             complement(49488..49943)
                     /locus_tag="IXG44_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1656940.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(50097..51305)
                     /locus_tag="IXG44_000196"
     CDS             complement(50097..51305)
                     /locus_tag="IXG44_000196"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HAQ1656941.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(51417..51749)
                     /locus_tag="IXG44_000197"
     CDS             complement(51417..51749)
                     /locus_tag="IXG44_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAQ1656942.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            51897..52772
                     /locus_tag="IXG44_000198"
     CDS             51897..52772
                     /locus_tag="IXG44_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ1656943.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(52854..53435)
                     /locus_tag="IXG44_000199"
     CDS             complement(52854..53435)
                     /locus_tag="IXG44_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IDEAL domain-containing protein"
                     /protein_id="HAQ1656944.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(53441..54700)
                     /locus_tag="IXG44_000200"
     CDS             complement(53441..54700)
                     /locus_tag="IXG44_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAQ1656945.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(54702..55748)
                     /locus_tag="IXG44_000201"
     CDS             complement(54702..55748)
                     /locus_tag="IXG44_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1656946.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(55983..56258)
                     /locus_tag="IXG44_000202"
     CDS             complement(55983..56258)
                     /locus_tag="IXG44_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008214712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HAQ1656947.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(56501..>57198)
                     /locus_tag="IXG44_000203"
     CDS             complement(56501..>57198)
                     /locus_tag="IXG44_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAQ1656948.1"
                     /translation="EGTTRDAIDTHFVSESGQKFLMIDTAGMRKRGKVYENTEKYSVM
                     RAMRAIDRSDIVLMVLNAEEGIREQDKRVAGYAHEAGRGIIIVVNKWDLVKKETNTMR
                     DFEQEIRDEFRYLDYAPIVFVSAVTKQRLERLPEMIEQVSMNQNLRISSAVLNDIIMD
                     AVAINPTPTDKGKRLKIFYATQVAVKPPTFVVFVNEEELMHFSYERFLENQIRKAFTF
                     EGTSIRIIPRRRK"
BASE COUNT        15706 a        11939 c         9782 g        19771 t
ORIGIN      
        1 tacttaaatg gtgactactc tgctgcaaac caagaaagag tagcagaaca atatgttact
       61 tcacgttatg gttcttggga agcggcgaaa gcattctggg aagcaaacgg ttggtactaa
      121 gattcattta ttaatcaaac agtttacttt tatacaaaga aagccaaaaa gaggtgcgac
      181 actagttgtg tcacatctct tttttattta cctaatctaa tgaaacgttc caagttttga
      241 taagtatttt ttttaacttc ttcaactgat agctctttga tctcagcgat tgtctttaac
      301 tgttcttcaa gcagtgagac ataatctttt tctgaaaaaa tcccccctaa agcccactct
      361 gctgcatgta gtccatccgt ttcaataagt aagtggtcga ttgggactgc tttagctagt
      421 tgccagactg ctggattctt tttcaaatca agaccaatgc tgaaataaca gcctaaatca
      481 atatagtctt gttgatagtc caaagaagaa taccaatgga tcaaatagtg attagggtaa
      541 gcttggatat aatttaagat ctctttttcg catccttttg tgtgcagtac aacaggtttc
      601 tcatttatgg ctgcaaaagc aagctgtcgc tcaaatactt ttttttgtgt agtcatcggt
      661 acattcgtcc aaatattatc taaccctatt tcaccgatga tccttgcttt tttcaaaaaa
      721 ggctcaactt gttcaaacgt atagctatct gctttccaag gatggatacc gtaacttaag
      781 gcttgagtct tacttgctgc aagctgtctg ttttccttcc attcttctgg cgaatcacaa
      841 ttgatgatcg tcaaaatcgt gtgttcctgc tgaaggagtg ctaaactctg actcccatct
      901 aaatgacaat gagcatcact taacatctaa ttcacctgcc aataactgat aaatcgtttg
      961 cttcaactct gattgctgca gtagccattc acgtgaccgt cgtttttcag gcacttgaat
     1021 ctcagcaatc acatgtgccg gacgggcgga aagcacaaga atcctgtctg ataaataaat
     1081 tgcttcttcg atatcatgcg tcacaagtaa agtagtccgc tttgcttttt taggcagctt
     1141 caacatccag tcttgcatct gccctctggt catagcatct aacgctccaa atggttcatc
     1201 aagaagcaag taagaagaag gattcatgat tgttcgtaac aaggccgttc gctgcttcat
     1261 cccgccagat aattgttccg gcaaaaaatg ttcatagccg tttaacccaa agatatccag
     1321 ataatccctt acttgttctt cattgattgt tagatgattg attttttgat ataacgtaac
     1381 gttttgatag acgctcagcc aaggaagtaa catatcttct tgcggcatat aactaaatgc
     1441 tgtatggaga ccagttactt tttgatcgtc tactttgatc gttccagaag tcataggctg
     1501 gactcctgtt aaagttttca gcaatgttga cttaccggct ccgcttggcg cgacaatcga
     1561 tgtaaaggta tctttaggga tagtgaaatg gatgtcttct aatattttca ggttttcaat
     1621 gactacattc aagtggctga tttggattga catatctatc actcctattt tataaactga
     1681 ttagtgaata gttcttgcga aggtacatct tgcttgatca atccgttttc ctgcataaaa
     1741 gctgtataat tgcgccaaac tttttcttcc atcaatcccc aattctcagg atcatccgta
     1801 taattttttg acaagaatgc ttgtgattct tctaaaaacg tccgatcatt ttcttttgca
     1861 tatttttgca acagtttagc actgtcattt ggatccttaa tagcttcctg atagcctttt
     1921 ttcgtggcat ccataaaaga ctgtaccaat tctggatcac ttttgatcag ttgatcattc
     1981 gtaataatta ccggagtata gtaatctaac cgttcatcta attctttcaa tgggatatag
     2041 tttagttcaa tacccgcttc ttttgctttg atcatatccc aaccttcaaa gtaccattgg
     2101 atatctgagg attttcctaa gctttctggc ccttctccat tgcttcctac cattgtcagt
     2161 tttgaaaagt ccccgccgtc tttttccatc acagctttta agacagcttc ttcacttgga
     2221 ctttgccaac ctgcatatgt ttttccttca aaatcttttg gtgaatggat gttactgtct
     2281 gatagggtgg cgaatccaga agtattatgt ttgatcactg tagcaattgc tttgactggt
     2341 atattctgtc catctgcgtg agcataagtg acatcttctt gataacttac accaaattgt
     2401 gctttgtccg cacctactag accaatactt gtcgaattat ctcctggttc gatgatttgg
     2461 acatttaaac cagcctcttt ataatagcct ttttcttttg ctaggtagat gccagtatga
     2521 ttagtattcg gtacataatc caaaacaagt gtcacttttt tcaaatcctt tatttcttgt
     2581 ttgctacttg tttcttttgc agtgccacaa gcggataaaa gcaacagtag cggtaataaa
     2641 agccataatt ttttcatttg atccccttct cttaaaaaat ataaagcata cgataatacc
     2701 cttttttcaa taatgaacaa gcgccattca gcaataaact caatagaatg acacaaatga
     2761 tcgcagcaaa aactttatcg atctggtagc tgttttttac gcgtatcatg taatacccta
     2821 agcctgcttg agcgcttagc cattctccaa cgactgctcc gctgacacaa taagtagcag
     2881 ccacttttaa accggaaaag aagccggata aacccgctgg tattttatat attcgataaa
     2941 tcttccctgt atctgccccc atcgttttca aaaagatgat ttgctctttt gatactttcc
     3001 tcaatgtatc cgaaaaagaa actacgatcg gaaaatagct tatcaagaca acaaggatca
     3061 cttttggaag cataccgaaa ccaaaccata gggtaagtaa tggccctaac accatgattg
     3121 gcaacatttg tgaaatcact agtaacggat aaaaagacat ttctaaaaac ttccaataat
     3181 ccattgccaa ggcagtaata aaagaaagaa cgctagctat taccaaacca agcactgctt
     3241 catttaatgt cgtgacagaa tgggaaagta atacttgtgc atctgccgct aatgcagaaa
     3301 atacagcagc aggtccgggt aaaataaagg caggtatttt ccaaactatg acaacaatct
     3361 gccaaataat taaaatgaat gccatactga ctgctggata aagatttttc ttcattttgt
     3421 tacctccttt cttctaatat gaataacaaa aaacgcttga aatgggcacg acatagtaac
     3481 ccgcttcaag cgtctgctat gtttctccct tcaccagtat taactgacag gttcaatggg
     3541 tctaagttca caacttactc tcatgtcgtc tacgacatcc ccagaaacag gtattcattt
     3601 tttaaagtgt agcacaagaa ggaaaaagca acaagcgctt tagttatcat gctgattatt
     3661 tcaataaata ggagagatca cttgccaatg tcggataccc catgatatag ctatccaact
     3721 cttttttagt caatttttga ttgatgacga gtgtcaaatc atcgatcaat tcgtctgctt
     3781 gctcgctaat cactgcagca ccaatcaaat aattttgctg gtcaaaggtc aattcagcct
     3841 tagcaactgg ttcattgaca cgatgatagg tgaaccaact ggtaagatcc atctctacca
     3901 cttgatctga cgaagaggcg tgactttttg taactcctac ttcagcaagt ttaggactgg
     3961 cgtagacgat cgtaggtata atcggatatt tgataggttc gcttgttgca tccgttatcc
     4021 gcttagctac ataatttcct tcaaaagtgg cgactggtgt caattttggg gttttccttg
     4081 aaacaatatc tccacacgca aaaatttttg gattgctcgt ttgcagataa tcattgactg
     4141 caattccgtg tttatcaaag acgatattcg cttgctcaag tgctaaagat tctgtattcg
     4201 gctgtctccc agtcgcacaa aaaatcatat ctgctactaa ttccgtctct tttccgacta
     4261 aacgatagcg agtaccttct gaaatgatct tttgggtttc aacaccaaaa gcaaactgaa
     4321 tgccagatgc ttccatttga tggacggctt cctctaccaa agacgcctca aattctttta
     4381 atggccgttg attatgatgg acaatggtta ctttgctccc tgcagcattg gcaatcgtag
     4441 ctagctcaaa agcaatataa ccaccaccga taaaaatgat ttctttaggc agtacaggca
     4501 atgataaaaa gtctgcacta gttttcaagt attcctttcc ctccactggt aagatcgtag
     4561 gtctttgccc ggttgccaaa actaagtaat ccgcatggaa gacttcttcg ttgacttcaa
     4621 tagatgaatc atccaaaaaa cgagcggttc ctgaaagatg atcgatttca gcttctgcta
     4681 attgtttttt tcggctttct gggactggat ccgtaaatgt tcgtttgaat ttttgaagtt
     4741 cttcccagtt ggcagttggg atctcgttaa aaccttttcc actcaattgt tttacccgat
     4801 tacgtgcctc tactgcactt aacaagactt ttttaggatc acatccacgg ttaggacaag
     4861 tcccgcccca tagatcttct tcgaccacta gtactgtttt acctgcttct ttcaatccat
     4921 aagcagcact taagccgctc acacctgaac cgatgatgat tgcatcatat gttttcattt
     4981 ccttcctcct ctcgacttaa aaaagcaaag atttctttac ttgtcagcga cccaatactt
     5041 ctttgctttt ctactatcac aatgcttgga tacaccgaaa gaagcgaata tacttctttc
     5101 ataggcgtat caaagcaaac ttgttgacga gcattttctg gtatttcatt taagaaatat
     5161 ccattgacga ctaaatcttc tactcgccac tctttcgtct gtttcggttc atcaaagaaa
     5221 tgccgaacgt agtcgttttt aggattcatc aataactgct ctggtgtgtc acattggata
     5281 atttctccat ccttcataaa tgcaatacga tcacctagct tgattgcttc ttccatattg
     5341 tgcgtaacga aaacgatggt cgtgcccaat tcttcgtgta aggacaaaac caattcttgt
     5401 aatgagtttc ttgaaatcgg atctaatgcg ctaaatggct cgtccatcaa aatcacatct
     5461 gggcttgcag caatagctcg gatgatcccg atccgttgct gttctcctcc agacaactct
     5521 ctaggcatac gatctcgata aatatcagga gataagccaa ctttttgaag aagttcatct
     5581 acacggtctg ctcttttctg cttttcccaa cctaacattt cagggatgac ttcaatattt
     5641 tgcttgactg tcatggtcgg gaacaaagcg atttgctgca gcacatatcc catattccac
     5701 cgcattttct gcagattata ttcatttaac gattttcctt tgaagtagat gtctcctgct
     5761 gatggtttag ataatccatt gatcattttc aaagtagttg tttttccact accggaagga
     5821 ccaaccaaaa caaagatttc tccttttttg attgccagat ttaggtcatc gatgacatac
     5881 tttccatcat actttttttg cacatgtttg aactcgatta atttagtcac tgatcttcct
     5941 cctttactaa tccttcattt ttcaggaaga ttcgtgctac ttctgtcggc tttttctttt
     6001 cgacattgac ttggtaattc atatcaatca tttgatcctc tgttattttt ccgcttaatt
     6061 tattcagtgc tttaacgatt tcaggatgtt ctttagcaaa atcttttttc atcagcggtg
     6121 cgccttgata agctgggaat aaccctcgat catcttctag agtcactaga tcgtactcct
     6181 tgagctggct atctgtggaa taggcatcca caatattgac atctccatta ttgattgctt
     6241 ggtaacgcaa acttggctcc atgctttgta cacttgaaaa attcaagcca tatgcttctt
     6301 ggattccttt atatccatct gaacgatcaa taaattccag tgtgaatccc gctttgatct
     6361 gatcagagat agctgccaaa tcggaaatct ttttaagttg atgttcgttc gcaaaagatc
     6421 tcttgactgc caaggcatac gtattttggt aagccattgg ttttaataat gtcatatcga
     6481 attgcttatt caataattct tctgcttcgt catatgtttt ttcttttgtc aaatcagcac
     6541 ttttcattgc ttcaggtacc ttcaccaagc tttctaggac agtacctgtg aactcaggat
     6601 agatatcgat ttgaccttga tccagtgcat tgaacaaaaa actcgtcttg ccaaaattcg
     6661 gttttaattc aacttctgtt tttgtgttgt cttcaatcaa ttctttatac atttggatca
     6721 ggatttcagg ttccgctcct aattttccag caatcgtgat ggtttcattt ttaaacaagg
     6781 cagactgata taaagaaacg ccgccaaata aacacaccac tgctgctaaa gtaatgatgg
     6841 aatatcgagg tttcttatgc tgcaaccagc gaatcagtcc gctgaatata attgcaagca
     6901 aggcagaact gatcgcacca atcaatgtca aaacaggtgt atttcggtca atacctaaca
     6961 aaataaatgt ccctaatcca cctgctccga tcaatgctgc caaagtagcc gtaccgatga
     7021 ttaaaactaa ggcagttcgg atacctgaga taatgacagg taatgcgatg ggaagctcta
     7081 ctttcagcaa gcgtctcatt ctggacatac caaatgctac cgctgcttct tcaatagacg
     7141 ggtcaatttc agctagacca atatacgtgt tttggaaaat cggaagcaaa gcgtaaataa
     7201 caagtgcaat caatgctgga acagttccga tccctacaaa tgggataagt aaacctaata
     7261 aggcaagtga aggaatcgtt tggagcacac ccgtgatctg caataagact tctgccattt
     7321 ttttatgatt ggctgcccaa atcgctaaag gaatagcaat cagtgctgca atcagcaaag
     7381 aaatgagaga aatggacaga tgttgaaatg cagcttccag caactcgcct tttctttcac
     7441 ttaatgtttg tataaaatta ttcatgcgac cttcctttct gttattcttt aagtataaac
     7501 atttctttcc tttttcccaa ctaacttgtc ttgccttaac cttacagcac gaacaaaaaa
     7561 aagaaaaaaa tacctctaaa tatgctgaca aaaaaagaat ccaagcaaca aagtcggatc
     7621 aactttttgg ttctcgtttt ttcactacga ataccataaa aatctatacg aaattgtcac
     7681 ttggaatcag ctatatcttt tttacacaca caacttttat tttaataaaa agaaacagcc
     7741 aataaagtag aaatctcgtg ggggagaaca cctttattgg cctgttcaac gaatattata
     7801 ctgtatcatg acactttttt gccaaaaatg taaaaaatgg ttgttttgac atcataaacg
     7861 tcttgttttc ttatttttac ttatatcacc actttatttt ctatctcttt cactccataa
     7921 agaggaatga gttcttcttt tgactgataa taaacagctt tctcacggtt cttcatcaaa
     7981 aaaagaaaga tgtctttcat agcaagtggt gctttgccgt gttcaaataa aaagataaat
     8041 tgatttgctt tatttatctg gaccattgtc acaggcacca gtttttgttc ttgagccaaa
     8101 aaaactcctt gatactctgt ttttttgggt gattttgtaa gaattgtcaa taagtctgct
     8161 gctcgcattt ttccactcct tatttgataa ataaaaagaa acagccaaaa ataatttttt
     8221 ttcattttcc tcttgaagta agagtataag tcagtataat aaagaagttg ctgaaaattt
     8281 tcaaaagaaa agaggctaga aatgaaaaaa ttaaatttat ttacagaatt gtgcggaatc
     8341 attttaggcg caggaatcta cggattagct gtgacaggaa tcaatattcc atctaagctg
     8401 gcagatggtg gggttactgg tattgctttg cttttgcata atctttttgg attcgctcct
     8461 tctgtcactt cactgctttt taatttacct ttattgctta tctcattgtt tctttttgga
     8521 aaacacgctt ttctccggac gatcgtcgga acatttgcgc tagttttctt tttacattta
     8581 tgggagagcc ttcatataca ttttgctgta gatagtctac tgatcaatag tttgatgaca
     8641 ggtattcttt ctggcattgg ttgcggtctc gtctttcgat tcggcggaag cacaggcgga
     8701 acagatatca tttatcaagc cattgaaaaa tattttcata ttaagattgg caaaagtctt
     8761 ttcttgatta cttttggtat tttagttgtc tccttgcttt atttggatct cacacatttt
     8821 ctgtatacat tacttagctg ttctattcta tcttatacat tgaataaagt gaagtatctg
     8881 gaaattggat ctgttttgcc ccaattggaa gcaaaacaga ctacagaaac atttgaacca
     8941 cttgatcctc ttgaacagtt agagcactga ttcttatttt tcactacttt atttaattta
     9001 cagaaatatc attatgtaaa ctaaaatgat attattcagt aaaatcttct ttataaaaaa
     9061 ataaactatt tttgcaaacg atctgcgaaa atagtttgtt tttttatttt gccattattt
     9121 tcttacttat tgggagaaaa aattcgctta aactcaaaaa aatttgagga tacgatataa
     9181 actttcggat agagtataaa ataaggaaac gccgcagcgt ttcctttcca actgtagccg
     9241 aatataaatt tgtatatcac ttatttaaaa tagctatttt ctcccaaaga gtttccttga
     9301 tcatcgatca atccttgtaa tgagcgttct aagataccat ggcttttttt cgattttttt
     9361 tcttctgact gctttgtttc ctctaacgtt tttgttcgat tgaatgttcg tacagacggc
     9421 tcaccatttc ttttttcttg atacggcgtc ggttctgtat cgaacaacag ataagagtca
     9481 tccgtttttt tcatcgattc gattaaatga tcctcagaaa ttttgctttc tctagggtct
     9541 gggataatcg atgcgggtac atatttagga acaaaataag aacgctcccg caccttcgtg
     9601 tcaacagatg cgtttcgtgt agtatgcttg gactctgtcc ttgtttcact ttgtctgaca
     9661 gaacgagagg aatcaccaaa aagttttttt cgtcctgtcg atgtgatttc tgattggtaa
     9721 tggccggtat aatccggtaa ttttccttta tgcttttcca aattttcctt acgcggagct
     9781 gacttcaatc catttgttgc acgacgcttg cgacggattt tctcggtgtt cgtgttcgtg
     9841 aaatgaaatt ctttcatctg ttcaaacggg gactgattct gttcagtcaa aagatcttct
     9901 tgtccgtcaa ataatactcg cttcttgtcg ttggttaatt ttacgcctaa ctcatcatcg
     9961 tacgcaggaa aacggaaatg ttttcgtctg atttctttca ttcaaaccat ccttctcaaa
    10021 aatggaactt ttctcaataa tagcacagat tcattgtttt cagtagcaat gcgctgatta
    10081 ttttaagaaa atagcgatga atttcataaa tatatatata gtaataagac ttaattcgtt
    10141 gtctctctga taactagttc aggaaaaaaa cgacgataac cttgactcgc ccctttttct
    10201 tctatttttt ttaacaacat ttctccacat acttcaccca tacgtataat atcttgcttg
    10261 atcgttgtca atttaggtga actgatttgg tctaaaaata ccccatcaaa tccaatcaca
    10321 ccaaattctt cgggcaagtg tcctccagca tttataatcc ctcttactgc tcctagtgct
    10381 agccgatctg aactacatac aaaacaagtt ttatctgtaa aatctgccca gttttctgtc
    10441 atatatcttt ctacttctgt cgaatgattt ggaaaccgca agattttact aggaagatca
    10501 acttccttca tcacagataa ataaccagtt tcccttgaaa tttcaaaagg ctcgtccact
    10561 tcgataccga tataaataat cgtttgataa tctctttcta aggcatattg cgttgctttt
    10621 gctgtaccat attcattatc gctgtctaca taatcatatc cttcagaatt ttccccaaac
    10681 aagacaactg gtttttccaa acggtcgatc caatcgaagt ctttttttct gacacctgtg
    10741 atgatgtatc catcacaaga accgacgtcg aaggcatcat ttgtaactaa ttggagagaa
    10801 taatgcgctt taccgatccg tttagcgatt cccatcagca aattcatgta atagggctca
    10861 gttgtgtcga tttcttccag tataaataat ttgatgatct gcgaccgatt cgtgacaagt
    10921 gcttttgcag caatatttgg acggtaatcc agttcagcca ttgctgagaa aactaactct
    10981 ttcaattcgt cagtcacctg ttccggatga ttgatgactc gagagaccgt catttttgaa
    11041 acattggcac gttttgcaac atctgctaaa gtgggcatgc tctcttcctt tctaacatgt
    11101 tgtcattctt actatcaatt ataccaaatt ttcctaacag agtttattat agcccaaaat
    11161 aaaaaacgga aaatcctcta tcgtcatgag tgattttccg tttcaattat ttatactttt
    11221 ttatcaaatt ttttgctaaa aaagtggaag cttcttccat tagtggtatt tgtaccatat
    11281 gtcgctgtgt ttttgaggaa cgaaaaacaa tttcttttcc gtaagactta tgccgatttt
    11341 tttctacaaa tcgttccgtt tgtatataag ggattttttc gtcagcttgt ccatgccaaa
    11401 agaacagagg acgtccagct aacttttccg gctgcaatga caaatcatag ctcttaaccc
    11461 atgaaatcaa atcaaaatag tcttttggta cgacataatt atattccgat gcgcgcctta
    11521 cgatttcttt tccatattcc aaaggtgcag gggaacccat caaacacacg cccgctacta
    11581 tatgaggatg tttagccaac aaggcgcatg ttgtcattcc ccccatcgaa accccgccaa
    11641 ctgcaagctg atcatgtaga agacctcttt tttctaaatg cttgataagt gtgtcaaatt
    11701 caaacagatt tccgtagata ctatcccaaa atgtatagga tggaatagaa gaaaccggct
    11761 gtttgcgctc cccatgattc atcgcatctg gcaggatcac acgaatgccc tgctgcgcaa
    11821 tttttcttcc ttgcgtcaaa actaactcct tacttgtctg ccatccgtga taatagatga
    11881 caacgggaat acgttcctct cttaattctt tcagaacaac ttctaacact ggaatatgat
    11941 ggacagaacg tttcaatata attggattca attgttactc acctattttc attaaatttc
    12001 ctgatcgcca tagcaaccat gctccaacag ttgtctggaa caggttcagt gtaccaaata
    12061 atataaatgc aggactgaat ggctcgggaa ataagcacag agaacaaagt cccaccactc
    12121 ctaatgctag cgaaaggagt ggataaatag aaaaattcaa aaaactggca acgctaccga
    12181 aaatattacc aataaacagt atcaaaaaga aaaaaacgct taaaattaaa ccatctgtca
    12241 gcgacatctg aaaaacaatg ttctctacct caattggctg ttgaatcgaa tagacaccta
    12301 agtatatact gagaaggaga cttagcacgt ggctggcaca aaacacacga ttgaccgttt
    12361 tcattttaat cacctaacct atctttataa ttctattata atcattttat catttttttg
    12421 tatgtataaa gtttcaattt ttcttcactt acgatttttt ttgatattgt tgttatataa
    12481 atgaaaggag ttttacaaat gattgatcca aaaatgttgg ggatgatctt cctcattaat
    12541 tttgcttata tcactctgaa cacgttacgt ttcatgttga caatgaaagg ctatcgactg
    12601 attgctccac ttgtcagtat ggtcgagatc acgatttata tcgtcggctt atcccttgtt
    12661 ttagatcgtt tagataatcc actgaattta tttgtctatg cgttaggtta cgcagtaggt
    12721 atcagtgtcg gaattttgat tgaagacaaa ttggcattag gatacatcat ggtcacaacg
    12781 attttgccag caaatagcgc agaagataaa agtttgccgg gtatactgag agaacacggt
    12841 tatggtgtga cacagacttc tgcaatgggg ctggaaggtg agcggctagt tctcgaaata
    12901 ctttctccta gaaaaagcga acgtgaactg tatcgcctga tcaaagaaat cgaagagcgt
    12961 gcttttatca tttcttatga gccaaaatat atttcaggtg gtttctggac taaaaaagtg
    13021 agaaaacgac aaggaaaata actcacaaac ttaaaaactg tctaatatct aaattaaatt
    13081 ttaaatgtta gacagttttt atttattatt ttgattaaaa aacatcgaca tatactcatc
    13141 aatttgttat tatactttat atacttatca atttaatttg gagatattat ggaaattcaa
    13201 cgtagaacca ctaatttttt acatttaggc tctttttttg caactttgac gatcttattt
    13261 tttctatgtt attcatacct taagacctca catgcgcctt ctagtgcatt aggctggttg
    13321 cttttggatc attctgatat cacagaagcc gaagaaaatc cgtttttttt gattttgtct
    13381 tctctttgcg gcttactgtt ttctgtttat ttcggtgcct atttttttca tcgacatcct
    13441 ggtacgttaa acgaagcttc aagcgtattg attgcattta aaacgttttc ttttttagca
    13501 attctttttt attcgtttaa tcacaatacg cttttcttct ttatcatgaa tggtttagtc
    13561 gtgattttat ccatttttac ttattttatt ttttcactca tttttagcca attgaaatgt
    13621 ccgctcttct ttcgattagt tcctgtcacc tgttttattt acagcttgtt ttcctttttt
    13681 gtccttgctt taatgccact gaccaaccct tcgacgatcc aagtcgcagg aaatctgttg
    13741 gatatgctct tcatcgtcag tctaggtgta tttatgtggc aaagaaaaaa caaacaaaat
    13801 aaaaagcatt ttgaagcctg actattacga cttcataaat gcttttttag ttttaaacac
    13861 ttcaatctaa aagaataacc caagattgga taaattatta atgactcgcc attcgtttct
    13921 tagtgattct ggattatcaa atggacttcc taatctaatc agctagttct tcccataaaa
    13981 tgccatactt gactgaatca taatattgat tatttaaaaa tctaactttt cttatcacta
    14041 cttcctttgt cattccacat ttctcaccat ttttttgcat tcctttgttt ccagaccatg
    14101 tagtgaatct aataggtggt agataggcaa aatactcaaa taattctttt aaccataaag
    14161 gaagagaaag gctcctcctg caagagtcag tataattttt gcttactgct ctcttaggtt
    14221 ttactcacta aaactgttcc atattctttg atataagaaa atgcatgcgg tataaatttc
    14281 ttgctcagct cagaaaagat ttgtcgttta agaaaggcca tttcctccat tgtcaatgaa
    14341 ggatccaatg ttggtaattc aatctcaaaa ttgttttttt cgacaatagg taattctaat
    14401 gtgaatgatt cttcttcctt tatagtattc gtcacaagta gagactgatg gaaaatacta
    14461 ggccggcgat ttttatattt attcttacgt atcctgtcaa cgataacttc tatttgatca
    14521 aaaaagtacg attcatttac gtctccaata agttcggcta attctttgaa tgccttcgtt
    14581 acaggaagct gattcttctg tacaaaaaag taattagcca aagtaagcgg gaagtgttct
    14641 atagaggaaa gtgtgataaa acggtacttc tcatttcctg cagttgtcct cagcaattct
    14701 gtctctcggc tgtttcttac tccataaaaa tacgcttctt tccgttcatc ttcgaaacaa
    14761 tacgcccaaa taacttctcc catcgctaat ttttgttcat caacagagag tctcttcaat
    14821 tgttcagcta tggtttcagc agccgaagtg gcttgttgca agtaatcttt aggatcaaat
    14881 attggaaagt tcaaatgata tcttcgttct tccctcttga ttaatcctgc atcgatcaat
    14941 aaatcaagaa aatgggaaaa tttattttgc gaaaattctt ttttcaattc tcttagaatc
    15001 gtttcttgtt ctcgatgttc atatagataa tcggtcaatg aagaaaaaag cggatcatcg
    15061 gtcagttttt gataaaagtt ttccttattt tttgaataat agttcatata tgttctcttt
    15121 tctatccgtt ttattttact taaaaggctt ttacgtaaaa aggaggaaga acaagagtca
    15181 taattgactg ttattctcct ccatttcttc ggcctttagt atgttctatg tttcttttta
    15241 tatcaatcgc ttatccattc attttgaatg cttcatacta ccatttgatt agctaaatac
    15301 agccgtcaat tgaggtacga cttgtttttt acgtgaaacg acacctttaa gcaaagcacg
    15361 gttgttggtc aaagttgttt ggaaagcttc ttctactttt tcttttcctt taccagcaac
    15421 aagtaattct gaatcactgt ctaagatgtt tgtgacaact aatacgaaca gatcgtagcc
    15481 atttgctttc atttcatctt ccattgctgc ttctagctct gcttgtcgat ccatcacttc
    15541 attaaggtcc actgtattga cttgagcgat acgaacgttg gaactgccca ttgggaaact
    15601 ttttgcatcc aaatcaagaa gaaccgcagc tgatttatca cttagatttg ttcctgcttt
    15661 caacatttca agtccataag tgtttagatc gacttcagcg attgctgcaa gttcttttgc
    15721 agctgcttca tcttcaggcg tacatgttgg tgatttgaac aacaatgtat ctgaaatgat
    15781 agctgagagc atgattcctg cgatttcttt aggaattgtt acttggtttt ctttatatag
    15841 ttttaaaata atcgtacttg tacatccaac tggttctgca cgatagtaca atggattagc
    15901 tgtttcaaag tttgcaatac gatggtggtc tactacagct aaaatattta cttcgctgat
    15961 atctgaaata ctttgttgga attcattatg atccacaagc atgacatctg cattttctgc
    16021 tttagctgac tctactactc gtggtgcaga tagctgaaag tggtctaatg cgtattgtgt
    16081 ttcttcatta ggtgtaccta gagcaactgc ttctgtttct ttacctaatt cattttgcaa
    16141 gtgtgaaaac gcaatggctg ccccgattgc atctgtatct ggattttggt gaccaaaaac
    16201 taaaattttt gacatgtcat tcgctccttt gtgatttctc ctatttaata atagcaaaaa
    16261 aacggaaaga agcaatactt atcttctttc cgatgcgacc tatcgcgtta aatattttgt
    16321 atactcttct gtatgaagca atcgtttagc attttcgaca cgttcttttg taggtggttc
    16381 gatcccttct aatggatatt tcaaaccaag tgtttcccac ttgtattttc ccatcgtatg
    16441 ataaggaaga atctctactc tgtctacatt tgacaaggtt tgaataaagt tgtctagtcg
    16501 gatcaaatat tcatcataat cacttcgctc aggaactagt acatggcgaa tccaaaccgg
    16561 tttgttgatt tttgacaaat attgagccat ttctaaaata ttttcattgt cccaatgcgt
    16621 tagtttttta tgctcttcat tatcgatgtg tttgatgtca aataacagta aatctgtata
    16681 cttcatcaat tcttgaaaac gtgaaaagaa aggttcttca tacgtaaaag gttttccgca
    16741 tgtatccaat gtagtatgca tcttccgctc ttttgctttt ttaaatagtt cgatcaaaaa
    16801 atcgatctgc aatagaggtt ctccaccact aacagtaata ccacctttat ctccccagta
    16861 aggtctgaag cgttctgctt tatctaaaag ttcatctgct gtgtattctt tccctccgcc
    16921 aatattccaa gtatctggat tgtgacaaaa ttggcaacgc atgcggcatc cttgcataaa
    16981 cacgacaaag cgcagtccag gtccgtctac tgaaccgaaa gtttcgatag aatgaacata
    17041 gccgattgtt ttttcttcca tttaagttca catcttttct ttgatttcat tttaagtaca
    17101 agttagctgt cgaagcgaag ctttctgaac aaaatttttc gcaattgccg cttcgttatg
    17161 tgtaaaattt acagggacgg aacatacaag tcctgcccct gtaaatcgtt tctttcaaat
    17221 aagcatactg attattcgat tttgatgcga tggctattca acgtcctttc gacgctaagt
    17281 cgaaaaactt ttttcacgaa ctttttacat tctgtcgtgt gaagttctca tcaagacatc
    17341 taattgttgt tctcttgtca agtcacggaa tttaactgca taaccagata cccggatcgt
    17401 caagttcgga tatttcattg gattttccat tgcatcacgc aataaatcat tactgaatac
    17461 attgacattc aaatgataag ctccttttga gaagtatcca tctaagacat tacgcaagtt
    17521 ttcgattctt gtatcttgtt cttttccaag acctgaagga ttgattgttt gggtatttga
    17581 aattccatcc aatgcacagc cgtaattcat tttagctgtt gagttcaggc tagctaataa
    17641 cccactttgt tccgcattgt aacttggatt tgctcctggc gcaagtggtt ttccagcttc
    17701 acggccatca ggagtatttc ctgtattttt accgtaaaca acatttgaag taatcgtcaa
    17761 taatgaagtt gttggttttg agttgcggta agttttatga cgtttgattt gtcccatgaa
    17821 gtattccaag atccatgaaa ctaattcatc tgcacggtca tcatcatttc cgtatttagg
    17881 gaagtcgcca tccacttcaa aatcaactgc tattcctttt tcattacgga ttgcttttac
    17941 ttttgcatat ttgatcgctg acaagctgtc tgccgcatga gagatcccag caatacctgt
    18001 agcaaatgtg cgttgcaagt cagaatccat caaagccagc tgagctgctt cataagaata
    18061 tttatcatgc atataatgaa tgatgttcaa cgtattgaca tatagttctg ctaaccaatc
    18121 catcatattc atgaagttat gtttcacttc ttcaaagtct agtatttctg atgtgattgg
    18181 acgatattct ggtccaactt gggcgccagt cacttcatcg actccgccat tgatggcata
    18241 taatagtgtt ttagccaagt tagcacgagc gccaaagaat tgcatgtctt ttccgacaac
    18301 tgttcctgat acacagcaag caatcgcaca atcatcagaa ccccattgtt cacgaagctg
    18361 attatcattt tcgaattgaa tcgaagaact ttcgatagcg attctgctag catatgttct
    18421 gaatccttct ggaaggtgtt cagaatacaa aacagtcaag tttggttctg gtgaaggtcc
    18481 catatttgtt aatgtatgaa gcagacggaa atcattcttt gtaactaatg aacgaccatc
    18541 taatcccata cctgcaatgg ataaagtagc ccagataggg aatcctgaga ataattggtt
    18601 atattcaggt gtacgcgcga atttgaccat acgtaatttc attactaagt ggtcgatcaa
    18661 ttcttgtgct tctttttctg tgattactcc attatccaaa tcacgttgga tatagatatc
    18721 aaggaatgct gagatacgtc cgattgacat cgcagcacca ttttgtgatt tgattgctgc
    18781 taaatatccg aagtatagcc attggacagc ttcttttgca tttgcagctg gtttagaaat
    18841 atcatagcca tatttcgcag ccatttcttt catttgcgct aatgcacgat attgttcagt
    18901 gatttcttca cgtaaacgaa tcacttcttc actcattgta tgataaccag tcatatcatg
    18961 atcttttttc ttttgttcca tcaaatagtc gattccatac aatgccacac gacgatagtc
    19021 accgatgata cgtccgcgac cgtaagcgtc tggaagccct gtaatgattt tgtttttacg
    19081 agcagcacgc atttcagcag tataagcgtc aaatacacct tgattatgtg ttttgcgcca
    19141 gtcagtgaaa atatgtgtca attcatcgtt tggtgtatag ccattgcttt ttagcgcatt
    19201 gttagccata ttgattccgc cgaacggcat gaatgcttgt ttcaaaggtt tgtctgtctg
    19261 tagaccgacg attttttcca aatctttatc caaatagcct gcatcgtgtg aagtaatagt
    19321 tgatacgatt tctgtgtcca tatcatagac accgttacgc tcgaattgga tatcatttaa
    19381 cttttgcaat tcatcccata atttttctgt tgcttctgtt gggccttcca aaaagctgtc
    19441 atcaccgcga tattcagtgt agttattttg aataaagtct ctagtgtcta cgccttcttt
    19501 ccatttggta cctttaaatc cattccattg ctccattgta tggacctcct aaatttctgt
    19561 tttatgtaac agtttttacg tgattagtat aacacagcac cctatctttg caagggtttt
    19621 cttgtttttt atttaacaat ttttatgttt tatttacata tatttaaatc aacgttgaca
    19681 taccaacatt taagcattgt gaacactttc atcttttttt gcaaacgtat aaggatttat
    19741 taaaaatgaa tgaaaaagag acgccacctg tattaataca gatggcgtct cttcattaaa
    19801 atactacaac agttacgact cgatgaaaat ctcaggagca tcgataactt cgatgacttc
    19861 tccacctttt ttctcatcca tcgcaaatga tccattcgat gttcgatcac ctatagggaa
    19921 atcagttggg tcaatcgtta actgctgccc tttttgattc atcaaggtta tttcttttga
    19981 tcttccttcc gacatataga taatacggtg cggatttttt ttcagctcac gcatgaccgt
    20041 cactccccgc ttcgcacgtg aagtttggct gatttcttga gcaagcatgc gtttaacagc
    20101 tcctcgctga gtaacgatta cgatcggcgt atcaccttct gctaacacaa gcagcccgtt
    20161 gataatccta tctccatctt tcaaattcat agacttcact ccagctgcct ttgctccaac
    20221 aacgggaaca tctgttaaag gatagcgcaa acccattcct tgatgcgtta ctaagaaaac
    20281 atctaagcct ggctgatctt ctttcagata cacagcaatc agttcatctt cttccgcctt
    20341 tagcttcata cagctgagcg gacggctctt ataggtgcgc catggttcga actctgtcat
    20401 tcgtgtttgc ttgatcaatc cattcttgct gatgaataca aatgttttct ctgcttgaat
    20461 tttttgatat gggaaaacag ctaaaatcga ttcgtctaca gacagattgg agattgtttg
    20521 agaaatgtgc tcaccagcat ctttccattt caaatcgggt agttcatgga cagggcggta
    20581 gatcacattt gctttatttg tgatcagaag aacatggtcc aatgtactca attcacctga
    20641 gtatagcaag tagtcgcctt ctcgcatccc gatttcttct ggtttggaag cactataaga
    20701 acgaaggctt gttcgcttca catagccttc tcgcgtcaca ctgacaatta cgtcttcttg
    20761 tgccactaag acttgcgtat caattttgat ttcttcgatt tcgtcttcga ttgtcgttag
    20821 acgagcggaa gaataatttt ttttcacttc tcgcaattct tttttcataa cagagaaaag
    20881 ctctttgtca tttgaaagga ttttattcaa ctctgtgatt tgcgcaatca agctttcgga
    20941 ttccttttga agctctgtga tatccgtgtt cgtcaaacgg tataattgca atgtgacaat
    21001 tgcttctgct tgttcttctg taaattgaaa gacgtcgact aaattctttt tggcgtcttt
    21061 tttgtcttta ctttcacgaa tcgttgcgat tacttcatcc agaatcgata aagctttcat
    21121 caatccttct acgatatgct gtcttttttg agctttggct aaatcgaatt gactacgttt
    21181 tgttatgact tgctttcgat gctcgatata acttcttaaa atgtctttca gtccaacttg
    21241 atgcggagtc atatggtcga tagcgaccat attgaaattg taattgatct gtagctcagt
    21301 gtttttgaac aaataattca aaattccttg agcattggcg tcttttttca attcgacgac
    21361 aatttgcaga cctgttctat cactttcatc tcgtacttca gctattccat cgattttctt
    21421 attcaaacgg atctcatcca tttttttcac caaggtcgct ttatttactt catatgggat
    21481 ttctgtgatc acgatttgct gtttattacc cttcattgcc tctatctttg ttttcgaacg
    21541 aagaataact tttcctcgtc ctgtttcata agcttttttg atttcatctt ttccttgcaa
    21601 gatccctcct gttggaaagt caggtccagg aatatattcc atcaattttt caaggctcgc
    21661 ttgtggatga tcgatcatat agattgtgcc atcgatcact tcagctaagt tatgagtcgg
    21721 gatttctgtt gcatatccag cagatatacc tgtcgatcca ttgacgagaa ggtttgggta
    21781 tcgagctgga aggactgttg gttctttttc tgtatcatca aaattccaca cgagatcgac
    21841 tgtttctttc tcgatatctg ctagcatctc tccacttaac ttggataatc tggcttcagt
    21901 ataacgcatc gcagcaggtg gatcaccgtc catactcccg ttattcccgt gcatttcgat
    21961 cagtacttcc cgcaacttcc agtcttggct catgcggacc atcgcttcat aaatactgat
    22021 gtcaccatga ggatgataat tccccatgat atttccgaca gattttgctg atttacggaa
    22081 tcctttgtca tacgtattgc catctttgtt catggcaaat aatatccggc gttgcactgg
    22141 cttcaatcca tcacggatat caggcagtgc acgttcttgg ataatatatt tggaataacg
    22201 tccgaagcgg tcacccatca cttcttctaa agttaattct tgtacttctt gtcgattttc
    22261 catcaggtga cctcctattc tacttcaaat aactgattgt tttcattttt atctgctcgt
    22321 tcttcatcta ataaatcatt agaaactgaa ggagagattt cagtatcctc ttttttatct
    22381 aagatactgc catcttcttc gagcgtaaat tgcacgtgat tttcgatcca ttttcttcga
    22441 ggttctactt tgtcacccat cagtgtggtt actcgacgtt cagcttgcgc agcatcatcg
    22501 atacggacgc ggatcaacgt acgggaagtc ggatccatcg ttgtttccca caattgttcc
    22561 gcattcatct ctccaagtcc tttatatcgc tgcagcatat atcctttgcc gactttttta
    22621 atcattgctg ccaattcgtc atctgtccac gcatattcaa tgacttgctt ctttccttgt
    22681 cctttggata ctttatataa aggcggtaac gcgatatata ctttcccagc ttcaatcagc
    22741 ggtttcatat aacgataaaa gaaagtaagc agtaatactt gaatatgggc gccgtctgta
    22801 tccgcatcgg tcatgatgat tactttgtcg taattacagt cttcgataga aaactcagga
    22861 ccgactcctg caccaatcgt atagatcatc gtattgattt cttcattttt caagatatct
    22921 tgcattttag ctttttcggt attgatgact tttcctcgta aaggtagaat tgcttggaac
    22981 tttctgtctc ttccttgttt agcagatccg ccagcagaat ccccttcgac taaatacaat
    23041 tcatttttct tagggtttcg ggattgtgcg ggtgtcaatt ttcctgaaag aagagattcg
    23101 ccttttttac gcttcttccc atttcggctt tcttctctgg ctttacgtgc tgcttctctc
    23161 gcttctctgg ctttgattgc tttacggacc aacatctggc tcatttcgct attttcctgc
    23221 aaataaaagc ccatttgttc actgatgacg ttatctacta cggtacgagc aactggcgta
    23281 ccaagttttt cttttgtttg cccttcaaat tgcagaagat tttcaggtac acggatagaa
    23341 agtacagcag ctaacccttc tctgaaatca ctaccttcca gattcttgtc ccgttctttc
    23401 agcaaaccaa cttttctcgc atattcatta taagatttcg tcatcgccgc tttcatacca
    23461 gcttcatggg ttcccccatc ttttgtccga acgttattga caaaagataa aacattttct
    23521 gaataaccgt cattgtattg ataagcgacc tctacttcga taccttcttt ttctcctgaa
    23581 aaataaacaa ctggtgtcaa tgtatctttt tcttcattca gataatcaac aaattctttg
    23641 attccttctt cgtaatgaaa aatttccttg actggttctt ctccacgaag atcagaaaga
    23701 gagattttaa cgccttttag aagaaaggca gattctctta accgttcggc taaaatttca
    23761 tatgaaaatt tcgttgtact gaatattgta tcatctggta aaaaatggac agtcgtcccg
    23821 tttgcttttt ttgtttttcc tatctttttc aaagtaccgt caggtttacc gccgttttta
    23881 aatttctgct gatactctac gccatctcta acgatcgtta caattagcca tttcgataag
    23941 gcatttacca cactggctcc tacaccgtgc agtccaccag aagttttgta ccctccttga
    24001 ccaaactttc cgccagcatg cagcacagta aaaatcactt caactgttgg tattccggaa
    24061 gcatgcattc ctacaggcat cccacgtccg ctatctgcaa cagttatact attgtcttca
    24121 tggatggtca catcaatctc actgccataa cctgataatg cttcgtcaac tgcattatcg
    24181 acgatttcat agactaaatg atgcaagcct cggctgtcag tggatccaat gtacatccct
    24241 ggtctttttc taactgcctc taatccttcc aatacttgga tggacgagtc attgtattcg
    24301 ttgtttactt tttttgccaa ggcaaacgct ccttcaataa tactttgcgg caaaaccgca
    24361 ccacgattat catactagaa aaggtcgtga aaaaaaagag ggtagacgag aaatcaattt
    24421 taattatctc ctaaccgctt tttcgacatt cccctctatc gatcatctct tctttgtggt
    24481 catttttatt tttggttttc tttcaactta ccaagttcaa tcttaataca acgatccatt
    24541 acgatatctg tacgtcctgc ttttcttagc atctcagctg cttctttgtt ttcaatccct
    24601 aattgtgccc agaacacttt agcatcagtt ttcaaaaaat cacctgctat ttcaggtaaa
    24661 aattcactcc gtctaaatac atctacgata tcgattggat catcgatttc tgtcaaattc
    24721 gcatagactt tctcgcctag aatttcttca ccagccaata atggatttac tggtatgatt
    24781 ttatatccat tgtcttgcaa tacctgtgca accatgtaac ttgttcgttc tggtttattg
    24841 ctcaagccta cgacggcaat attttttgct tcttccaaat agttgaaaat cgtttcttgt
    24901 gttggatttt ttacagacat ttctgccacc ccttgctttt ctttttattc catctattat
    24961 acttgtatct ttcatttttt caattgttcc tgttacctat tcatgctaaa ataaaggata
    25021 gttttcaact tgaaagaggg taaaaaatga aattgatctt attacttatt ttagcatatc
    25081 ttttaggttc tattccttcc ggtgtctgga ttggcaaatt gtttttcaaa aaagatatcc
    25141 gacaacacgg gagcggtaat actggaacga ccaatacatt tcgtgtatta ggcaaaacag
    25201 ccgggattgt tgtcctgctt atggatattt taaaaggtac attggctact tgtttgccga
    25261 tgatcttcca cctttctggg attaaccctc tttggtttgg ggtatgcgcc attcttggcc
    25321 atacatttcc gatttttgct gggttcaaag gaggaaaagc tgttgcgaca agtgcaggaa
    25381 tgcttttagg atttaatcct gtattttttg tttattcttg tatcatcttt atcgttagtt
    25441 tattttgtac aagtatggtg agtttgagca gtatgatcag tgcagtattg atcactttat
    25501 ctacgatcgt ccttccttat gtagctcctg tgattttggc taagcccaat tggttactga
    25561 cgatcattgc ctttttagtt tcttccttta tcttttatcg gcataaagac aatatcaagc
    25621 gtatcagaaa ccatactgaa agtcgaatcc cgtttggttt gaatagcagc aagaaaaaat
    25681 cctgatactt tttcattcca aagagataaa acgggagtgt gacaaatcga tgtcacactc
    25741 ccttattact tacaggatag cttttttaca caccttggta aacggtgttc cctgctaact
    25801 tctcggtcac aacctcttac ttcactgtga tggaatattt tgtcttgaag atttcatggg
    25861 cacctagttg attgatccct actttttcca tcaagtttcc actactgttc accgtgtcag
    25921 cgattccgca ccacggttcg atacaaacga aaggcgcttc ttttggataa ggagaccaga
    25981 tccctacata aggcatatct ttatagctta aagttacgct atgggaagat ttttcacttc
    26041 gaatggcgta cgcatttaga ccccttgttt caaaaaccaa ggcatcttgc tcaaaaagtt
    26101 cacgattcaa agccagattc gtatttgtct gacctaacgt cttttgatcc atatcgatga
    26161 atgggccttt caatggtagg cgtaatcttg atttcaatgg agaaaatgcc agatagaaat
    26221 cttcaaatga caatccttct tcaagaggta cattgaaagc aggatgtcca ccgatagaaa
    26281 agaacatttc tttttcaccg gtatttgcca cttgataatg aaccgtgatg ccgtctcctc
    26341 ctaattcata gctgatcact aattcaaaat caaatggata cttttctaat gtctccggcg
    26401 tactccttaa gatgaaacta gccatttctt gtccatgttc aatgacttca aaatccatat
    26461 cccgagcaaa tccgtgttgc cccatttgat atgttttccc ttgataaaca tactgatcat
    26521 cttttaaacg tccaacaaaa gggaataaaa ccggtgcatg tctgccccaa tacgctggat
    26581 caccttgcca gatatactct aaaccatgtt cttttgattt cagactgatc aattccgcac
    26641 cgtgttcagc gatttcggcg atcagctggt cattttgaat aataacagtc attgccattc
    26701 ctccaacttc gacttacttt ttaaaatggg aaggctggaa caaacatttc tatctgcccc
    26761 agtcccctta cttctaataa ctgtgttttg ctaactcgtc tttaaaacgg gaattcaata
    26821 cttttaaata agttcccttc atgcctaacg aacgagattc aatgattcca gctgattcca
    26881 attttcgtaa agcattgacg atcacagaac gagtgatgcc gatactatcg gcaatattag
    26941 aagcagtcag acgaccttca tctccatcca atgcttcaaa gatagcttgt acagccttca
    27001 attcgctata agataatgtg ttgattgcca tttggacagc cgttgcactc cgtacatctg
    27061 cttcgatgct tcgagacttc tgataaagga tctgcatacc gacaaccgta gctccatatt
    27121 ctgctaagac tagatcgtca tctgcaaacg attgttccat acgagataag ataatcgtac
    27181 ccaaacgttc gccagctcca aagatcggaa cgacagtcgt cagaccaaat ggatatttct
    27241 ctcttgactc taccggaaat gcagtaatat cactagtaat tgggatatta gcttcagttt
    27301 tcatcaactg tgccactgta tcagtataag attcaggaaa ttgtttctca acaaacattg
    27361 ttttgattcg ttcattgttc acatcgtgac gttcattata acctagtaat actccgtcac
    27421 ttcctatgat ataggtgttg ctgtcaagaa taccacctag tgtgacagcc attttatcgt
    27481 aaggcaaatc tgcttgcatg tcaaaagtat tcttttgctg caaaagtgtg tttatcttgc
    27541 gggttttcgt tagcaagtct gtcattgtcg tcctccgatt ttttataaaa tatagcgact
    27601 taaatcttca tttgccgcaa ttgagccaag tttgtcatcc acatatgctt ctgtgatcgt
    27661 tatttctccc atttgcatat ctggtgcttc aaataataga tcttctagta gtttttccaa
    27721 aatggtatgc agtctgcgtg cgccgatatt atctgtgtca cggttgacat cgaaagcgat
    27781 ttgagcgatc cgttcaattg cttcttttgt aaacgtaact gacacatttt ctgtaccgat
    27841 caacgcgacg tattgtttta ctaaagcatt atttggttcg gtcagaatct tcacaaaatc
    27901 ttcagctgtc aaatcatcta attctacccg tatcgggaag cgtccttgaa gttctggaat
    27961 tagatcactt ggtttagaca aatggaaagc tcctgaagca atgaatagga cgtgatctgt
    28021 ttggatgatc ccgtattttg tgtttacttg tgaaccttcg acaattggca atatgtctct
    28081 ttggacgcct tctctggata cttctccaga ttgctgtgat ttagaagtga tcttatcgat
    28141 ttcatcgata aaaataattc cactttcttc cgctaatttg attgcctcac tatggatatc
    28201 agcatccttg actaattttg ctgattcttc cttgatcaat aactctaaag cttctttgac
    28261 tgttaccgtc cgttccactt ttttcttcgg tgtcaaagca cctagagttt catttaggtc
    28321 gatccccatt tgctccaatc cattgttcat cattggtgca ggtttctttt ggtcttcaac
    28381 ttcaatggtt acttcacgac tatccagtaa acccttttgt agttgttcca agactgtacg
    28441 gcgattgatt cgaatatctt ctgtcacttc ttcttgcggt tcagcggtat tttgctgact
    28501 agcattaaaa agattcatca tttgttcaaa ttgattagtg gattgtttct gttctttttt
    28561 gataccaggt accaagattt ttaccaaacg tttttctgca ttttttttag cacttgtgta
    28621 tacacggctg tactgctctt tttctacgat ttggatcgca ttttctacta aatcccgaac
    28681 cattgattct acatctcttc cgacataacc gacttcagta aattttgttg cttcgacttt
    28741 gacaaatggt gcattgacga tttttgccaa acgtcgtgcg atttctgttt taccgacccc
    28801 tgtaggtccg atcatcagca tattttttgg tgtaatatct tgttgcattt tttcatctaa
    28861 ctgcaaacgg cgatagcggt tacgcaaagc aacggcaaca gatttttttg ctgcgtgctg
    28921 tcctacgata tattcatcta attcacttac gatttgtctt ggtgtttgtt ttaattcata
    28981 catatctact cacctcttac agttcttcta cgataatatt atgattagta aagatacaga
    29041 tatctgcggc aatatttaac gcattttctg caatttcttt cgcactcatg tcgttttgag
    29101 catagttttt cattgctcgg gctgcagcca acgcaaaatt ccctcctgat ccgatagcta
    29161 agataccatc atctggtgtg ataacttctc ctgtacctga aacaagaagc atttctttat
    29221 cattcattac gatcagcatc gcttcaagtt tttgcataga ttgctgtgtc cgccattctt
    29281 gagctaattc gacagctgcg cgttgtagat tcccgttata ttcgtttagt ttgccttcaa
    29341 atttttcttc taaagtaaac gcatctgcaa cacttccggc gaaacctaca acaacttctc
    29401 cattgtagat acgtcgtact ttctttgcag tgcctttcat gacaacagat tcacccattg
    29461 ttacttggcc atctccagcc atagcaaatt tcccgtcttt ttcgacggca cagatagtgg
    29521 ttgaatgaaa tggtgattcg accatgtttg tttcctccta aatttgttct tttccttatt
    29581 tttacgctct tggatggaaa gaacgatagt ttttttgtaa actttctttt gttacatggg
    29641 catagatctg ggtagtcgat aaattggcat gtcctagcaa ttcttggact gtccgcaaat
    29701 ctgccccatt atttaataaa tgagtagcaa atgtatggcg cagcatgtga ggatggatat
    29761 cactatccaa actgcttttt ttgatcattt gattcaagac atattcaatt ccagtaggcg
    29821 tgattggttt tccatgatga ttcacaaaaa cgatatcatg ctcttcgtga tattttgtca
    29881 tcaatacctg gcggccttcc ctgaaatatt cttgcagagc atcttgagca aaagaaccta
    29941 gaggagcata tctctctttg ttccctttac catggatcaa catgacctct gaatcaaaat
    30001 caatgtctga taccgacaaa ctgctacact cacttaaacg gatgcctgac ccgtaaagga
    30061 cttccagtaa agcgcggttc ctcaaatcca aaggactatc tcctgctacg ctgtcaaaaa
    30121 gtgcttgcat ttcattttca taaaaaaaac gtggcagtct caaatttttc tttttgagat
    30181 gaacgtaaga aaatggattt tcttttatca cttcttcttt gagaagatac tgataaaacg
    30241 aacgtaggct ggctatcttg cgactgatag aatttctgct atagctagga cgatgatcag
    30301 ctaatgcact caaatagaca cgaaccgtaa aatattccgc cttcagcaga tcagcgtctc
    30361 cagattcctc caaaaaatgt ttgaaatgga gaatgtcttc ttcatatgct tctttcgttt
    30421 tttctgaata tcctctttcg acaatcaaat aacgcaaaaa tttttgttcc cacgtggctt
    30481 ctgtcatttt ctcaccttcc ttttgtccca gagaataatt tagcacagcg tttataaaaa
    30541 gacaaataaa ttgtcagaat ttaaccaata tttctaagtt tgttcacaat tggcgcaatt
    30601 cctcacattt tgtttgagaa attcttaaga aaaacgcaag aaaaagagcg agactatccg
    30661 tctcaccctc ttcatggagt ttccttactc atttgggatt gcagtttttt ctaattctgt
    30721 ttttattgcc tcattagctt ttaaagctcg ttgtgcaatt gcttcatatc ttgctttttt
    30781 gtctcgaatg cgctctggca gttcaggaaa cagaccatag ttggcattca tcggttggaa
    30841 atgttttcct tccgcatgag taatgtagta agccatactt ccaatagctg tttcttgtgg
    30901 aaactcgact ggttccattt cttttgctaa gcgtgcggcg ttgattccag caagtagtcc
    30961 gctcgctgca ctttcaacat agccttctac accagtcatc tgacctgcaa agaacagatc
    31021 ttcgcgtttc gttgattggt aagtcggttt cagcaattct ggcgaattca taaagctgtt
    31081 tcggtgcatc acaccatagc gaacgaattc agcattttct aacccaggaa tcatccggaa
    31141 aacacgtttt tgctcgcccc atttcaagtg agtctgaaag cccacgatgt tgtataaaga
    31201 agctgcagca ttgtcttgac gcagctggat aacagcataa ggacgtttgc cagttttagg
    31261 atcttccaat ccaactggtt tcataggtcc gaaaagcatc gttttgaatc ctcgctgtgc
    31321 cattacttca attggcatac atccttcaaa atatttttct ttctcaaaag atttaagttc
    31381 tgccacttcg gcattgatta gtgcctcatg gaaagctttg aattcctctt ctgtcatcgg
    31441 acagttcaag taagctgctt ctcctttgtc atagcgggac ttcaaatata ctttatccat
    31501 atcgatcgta gctttgtcta cgattggtgc agctgcatca tagaaataaa aaccatctga
    31561 accattgaat gctttgatgc tttctgctaa tggatgagaa gtcaaaggtc ctgttgctac
    31621 tacggtgatt ccttccggta tttcagtaat ttcttcgttt atcactgtta ctaatggatg
    31681 ctctttgact tttcgggtaa ttgtttcaga aaaagaatcg cggtcaacag ctaaggcacc
    31741 accagctggc actgctgttt catctgccga agtgattact accgaattca atcggcgcat
    31801 ttcttctttc aataacccta ctgcattagc caaactgttt cctcttaatg aattggaaca
    31861 tactagttca gcaaaattac ttgtgtgatg cgcctcagtc gatttgactg gacgcatttc
    31921 atataaacgg acagggactc ctgcttgtgc gatctgccaa gcagcttcac ttccggcaag
    31981 ccctgctcca acaacattta ccattttcaa tgaaagaacc tcttttctat tatttttgga
    32041 tgtcttcttc gtaatctcca tttacacaga ctacttgttt accgcctttg actttcttct
    32101 cgaccagata tccgtcacat tttggacaat ttctgcccac tggtttatcc caagaagtga
    32161 attcacattc tgggaaacgg gaacaaccat agaatagtct gtttttctta gattttcgtt
    32221 caatgacttg tccttcatga catactgggc aagtcacacc gatttctttt acgattggtt
    32281 tcgtattccg acaatcagga aaattactgc aagcataaaa cttcccatat ttccctaatt
    32341 ttatcaccat cggatgtcca cagacatcac aatcgaatcc tgctggttca tctttgattt
    32401 gaattttttc gattttttct tccgcattcg tcagttcttt ttcaaacggt ttatagaaac
    32461 gatcaaccac tttgacccac gcttccgtgc cttcttcaat tttatccaaa tctccttcca
    32521 tttctgctgt gaaatggata tcgacgattt gagggaagaa ttcacagatt aatgaattga
    32581 caatttctcc tagttcagtc ggctcaaatc ttttttgtgt caattttaca tagtagcgtc
    32641 tttggatcgt atcaagtgtt ggtgcataag tggaaggtcg ccctaccccg ttttcttcta
    32701 gtgcacgaat caacgtagcc tcactaaaac gtgctggtgg ttgagtaaaa tgttgttttg
    32761 gttcaatatc aacagattgg actacatcgc cttcttcaag ttctggaaga atattttctt
    32821 tctcttcttt gccgtcatct ctaccttcca catacacttg catgaatcct ttgaatttga
    32881 tttttgaacc gttcgcaatg aagatgacac cattttgttc taatgtcact ttcattgtat
    32941 ctaaaatagc tggtgtcatc tgactagcca caaaacgtga ccagatcaat gtataaagtt
    33001 tcaattgatc tttatctaaa tattgtttga gttcatctgg tgtacgaagc acactcgttg
    33061 gacgcacagc ttcatgggca tcttgtgcgc cttgagcatt tttgatcttt cggtgatcat
    33121 gtctagaaaa ttcttttcca tacgtatctt caataaatgc tgcggcctct gcttttgctg
    33181 tatcggcaat ccgggtagaa tcagtacgca tgtatgtaat caagccgact gttccttgtt
    33241 tacccaaagt gattccttca tataattgct gtgctaccat catggttttt cttgttcgaa
    33301 agtttaactt acgggcagct tcttgttgca ggctacttgt agtgaaagga ttcgcagggt
    33361 ttcttttgcg ctctttcttt tctacttttt taacttcgaa gtctgttcca tcaattctag
    33421 cagtgacttc ttttacactt tgtgcatctg gtaatttctt tttcttaccg tcgattcccc
    33481 agaaatttgc tttgaatttc ttgcgagctt ttttaaaatt accgtcgatg ctccaatatt
    33541 cttctggaac aaattctcgg atctcttttt cacggtcaat aataattttt aaagcgacag
    33601 attggacacg tcctgcactt aaccctttct tgaccttacg ccagagaata ggactgatcg
    33661 aataaccaac gattctgtct aaggtacgac gagcttgctg tgcatctact aaatctacat
    33721 cgatcgttct tggttcctta aaagctgctt tgactgcgtc ttttgtgatt tcattgaaga
    33781 ccacccgatt tttttctttc agatccaacc caagtaggta cgctaaatgc caagcaatcg
    33841 cttctccttc tctatccggg tcacttgcca agtaaacttt ttgagctttt tttgctgcgg
    33901 ctttcaggct tttgatgaca tcgccttttc cgcgtataga aatataatgg ggttcataat
    33961 tgttttcaaa atcgataccc attttacttt tcggtaaatc tcttatatgg ccaacactgg
    34021 ccattacttt atagtttcgt ccaagatatt tctcaattgt cttggctttt gcaggtgatt
    34081 ctacaatcac taaatattta tacgccatta ctgaatggac tcctttccat tgtcgtgttc
    34141 ctactattaa aagaggattc atacaaatcg tagctaatca tatccattca gaaaacgttt
    34201 gtcaagaaaa gcaaatgaaa agtatggtgt tattctttaa attctgccaa gatatcttca
    34261 cccgaaaccg tgcaaactgc accgtcttgg atcaaatgat gacatccggt agaatgttct
    34321 gttaaaatat ttcctggaac agaaaaaact gttcttccaa gatccaatgc ttgctgtgct
    34381 gttatcagag agccgctttt tttcttggct tcaaagacac atgttccttt agacaaagct
    34441 gcgataatcc gatttctcat tggaaaacaa tgctttttcg ggccggattg aggaggatat
    34501 tcactcaaaa gaagatgatc atttgcaatc acctcttgca agtcacgatt ttcagcagga
    34561 tagacttgct ctatccctgt tccaatgact gcgattgtcc gtccaccatg agagattgct
    34621 gcttggtgac cgtatgtatc tatccctttc gcgcagccac tgacgatcac atatccagct
    34681 tctatcactg gttttagtaa ataataaact gtttttttgg ctaaagcaga agtgtgtctt
    34741 ccaccaacaa cgcttaacat gttacaagaa agtaaagata gatcaccatt ataaaacagt
    34801 acaattggcg gataggtcaa gtgaagcagc tgagggggat atttgggatc aaagcaagtg
    34861 ataaacgact gtttcgattg gattttgccc cagttagcgt gaatttcctg ccaacttttg
    34921 cgaaacagct cccggtgttt ggttatttta cctatcttaa tgatgtcttc aaatccgatt
    34981 tctttataag gatactgcat catccagtta gcaagtgccc attttccttg actactgatt
    35041 ccttttgtat aagcaagttt caataaattt tcttctattt gatacatcat aaaaccgcct
    35101 ttcgtctctc tttctaataa gatacgtaaa agcggctgtt ttttattgta aatatttttt
    35161 tacaggtgca aaacttttac gatggattgg cgtaattcct tgtcgttcaa gtccttctaa
    35221 gtgttccttt gttccataac ctgcattatg cgagaaacca tatccaggat aaagcacgtc
    35281 atattctttc atcaattggt ctcgatatac tttagcaatg atacttgcag cagcaatcga
    35341 cacagatcgt gcatcccctt tgattatttt ttcctgctga ataggcaaat ccaaggtcat
    35401 cgcatcgatc aacaaatagc ttggtacaat ggacaatttt tctactgctt ggatcatcgc
    35461 taatttactt gcttcataaa tattcacatc atcgattgtc tgctgatcaa cgatcccgat
    35521 cccgattgcc agggcttttt cttgaacttg ttgataaaga agttctcgct ttttctctga
    35581 taattgtttg gagtcattca gccctaatat gatctcattt ttcggcaaga taactgcagc
    35641 tgccacgaca ggtccagcta atggtcctct tcctacttca tcaatgccgg caatccattg
    35701 gtgaccttgt gcgtaccctt gcttttcaaa gcaaaacatt tcttcatatt tttccttaat
    35761 taatgcttcc tttttcaatc gtcgttcaaa agaagccagt gcttgctgca cgcctttgcg
    35821 tggatcattt cttaatgatt ctagtgtagg atcatctatt ctctgtatgc ttttcaatcg
    35881 ttctttgata ctttggatgg attcagtcat cacttttttg ctccatttct tcgcaacgat
    35941 ccagtgtgta tcgtccaagt tttccttgac gaatatcatg cacgactaat tcgctgccac
    36001 gatcatagtc ttctttatac ccacgttttg ctgtaatatc catcagtaat tccggtgcca
    36061 atttttcttc ttgctcgatt gataaaccat aacgatcgct gattcttcct gaataatatc
    36121 gggcaaaaaa gtctaatccg taaatcgcta aatcatctaa atggacaagc tggtctttga
    36181 tcgcgcctgt cagcgctagt tttttgccga tttcttcatc ttcaaatttg ggccataaaa
    36241 tccctggtgt gtctaacaat tccaactcat tacctaaacg aagccactgc tgaccttttg
    36301 ttacgccagg cttatttcct gtctgtgcaa ttttcttgcc tactagtcga ttcatcaacg
    36361 tagatttccc aacatttgga atacctaaga ccattgcccg aatagcgcga ggcttgatcc
    36421 cccgagcagc atctcttgcc cgtttttctt tcaatgcttc tttagccgca ggtactagac
    36481 ctttaattcc tttactttct tgtgccgtga tagctaatgc aagatggcct tgtttacgaa
    36541 aatattcttg ccacagcttt gtctgttgtg gatctgccag atccgcttta ttcaataaaa
    36601 caaggcgagg tttttgctga atgatctgat ctagcattgg attacgagaa gataaaggta
    36661 atcgtgcgtc taccaattca aatacgatgt ccacatattt cattttttca gaaacttctc
    36721 gtcgggcttt tgccatatgt cccggaaacc attggatggt cattgttctc tccattcctt
    36781 tcataccgga atattccccg gctttacttg tttttttctt tttcaatata aattcacttt
    36841 tttattataa agaaattcca tctattagtt aagtgaaagt gaaccagtcc tcattctatc
    36901 acatcttcac ttttttgatg atgcaaattt ttattttggt ttctatatat gaatctaaat
    36961 ttttgacagc gtttcctaaa taagagatga tagacgaaca aaacagaaag gagacgaaac
    37021 aaatgaatag tatggttttt gttaattttc ctgttgctga tgtcaatcgt tccgttgctt
    37081 tttatgaaaa gctgggtttt aaaaaaaatg aagaattttc tactgaagaa gctggttcaa
    37141 tggtatggga tgacactttt tggataatgc ttctctctca tgatttttat aaaaaattta
    37201 taaaagataa agaaatagct gataataaaa aaacaagtgg tgctttgatt tcgtttagtg
    37261 ttgaaaatgc ggatgaagta aaaaaatttg ctcaaattgc aaaagaaaat ggtggtactt
    37321 tccacgaagt agatatggga atgcctgaag aagaaatgtt ttcattagaa gtccaagatc
    37381 tagacggaaa tacattagaa cccgtatgga tgaagatgta agcagataac taatctgacg
    37441 ttttttataa caaaaaaaca aaggcggatg atacgataag tattctccgc ctctattttt
    37501 tttattcgta caatctcttt tttacagttt ataaaagcag tcttctcacc ctttttttaa
    37561 ttgttagatg ccagtgtatg taaatcacca atgtgtcatg acacattggt gattttatgg
    37621 tatcctttta ttattaactg acaaaggaga gaatattttg aacactttga ttgcaaaaga
    37681 cgtttttaga ttgcccgaaa tgaatgcaag attgttgtcg acctctgctc ttttattagc
    37741 ccttgatgtc atcttataca agatagcgat tggtcctgct tattttcaaa tcacattcgg
    37801 atttttaagt atggcgctta tgggttatct atatggaccg atctgggctg gtttgcttga
    37861 aatgttgtcg aacactttaa atttcacgat atttggttca ggaacgtttc aagctgcttt
    37921 tcttatcacg gctttccttg gtggatttat taatggctta tttttatatc ataaaaagat
    37981 aaacctgcct tccgtcatcg ctgcccaatt attgattatg atcccagtca gcctttttat
    38041 caattcttgg ctgatgtctg ttttgtttgg tgcagatttc aaagtaattt ttctaggtag
    38101 attcatcagg aatatgatcc aaatccctat tcaaataatg gttgtttatt catttatcaa
    38161 aactttagag gctagaaaag tgtttaaacg agctgtttga ttttacgtac taaggtagta
    38221 aactgatttt ggattaaaca ataaagcgtg cagtctcaac agactgcacg ctttattgtt
    38281 tctcactgga tccattttat ttcatcaagt ggataataga caaatcttgc tttacctaga
    38341 atatcagttt tattgatcgt gccaaacgaa cgactatctt ttgaaactct tcgattatct
    38401 ccaagtacaa aataattatc ttccggaagc ttggattgtc ccgttagctc ttgtagtgta
    38461 aagtccgtcg tataggatgc atttgcatga tctgatttca ggtttttagt tagataaggt
    38521 tccttaacta ctttcccatt gatcttcaat tgatcatttt ggtagctgac attttctcct
    38581 ggaagaccga ttactcgttt gatataaatc gtgccatctg ctaattgaaa aacaacaata
    38641 tcaaatcgtc tgatctccga aaatttttcc atcacaacca tatcaccttg cttcaacacg
    38701 ttttccattg aattgccttc cactggtacc gggatgaata aaaatccccg caacatgaac
    38761 gcaaggaata cagaaacaag taagtatttg aatacaagcc agaagcgatt aatatagcgt
    38821 tgtttttttg tcaatctcta tcaatccatc cctctttttt tcattctttc tttattataa
    38881 gaaaaatatc aaaaaaatgc gactagatag ctgaaataaa aataattctt tcaattaggc
    38941 tattggacaa aaaacttttt tgatcataaa gtacaaaact atctcttata tgtcatgatt
    39001 attttctaaa tatttttccc atgcttcgtc aaagatatcc atggacggca aatagtcgac
    39061 attcagttcc agataattag aaatttcttc gtagctagag ctgtgcttag gaaattggat
    39121 gtctttaccg gcttctgtag cgaaaacaga taaagcatca tgtgtgccac cgcctcgcag
    39181 tgtcattaaa taatgaaaaa agcttcgatt cacccttcgt tcctccaatt ttcttcgata
    39241 aaggcagccc ggcgtgcatg gtttctttta aaattttcag gatgcttttt ataatactct
    39301 tgatggtctt cttctgctag ataaaatggt tgggctggtt cgatttttgt aacgattggc
    39361 tgttcgaaac gtccactttc agctaactgt ttcttacttt tttcagcaat gatccgctgt
    39421 tcttctgtca aataaaagat caccgggcga taattgtcac cgcgatcctc gaattgtccg
    39481 aaagcgtcag ttggatcagt ttgctgccag taaagttcta ctaattgttc atatgaaatc
    39541 aatgttggat catagataat ctccactgct tctgtatgtc cagtcgtttg gctttttact
    39601 tgttcataag tcggatctgg tacatgccct ccagtatatc ctgacatcac tgtatggata
    39661 ccaggaagcg tgtcgaaagg ctggatcata caccagaagc agcctcctgc aaatattgct
    39721 cgttccatta tgattccccc tctaatacta ctcatccgat gattttttat tttcttttgt
    39781 cttgctttca gtactgctgg tgctagtttt gttggtattt gagttgtttg aagcatcaga
    39841 ggtatctttt ggcaaatata gactaaaccg aatgtcatca tttactaagt cgattttatc
    39901 tgctttaata tacatcccgt tttgcattcg gaattgatcc aacctaagca tgatagtcag
    39961 atcatctgta ttgacttcga tccaatctgg taatttatag tttctcttga tcatattcat
    40021 cacatctttg attggcaaac tcaatgttcc gatagaaaga cttctagctt ttagttggac
    40081 atttccgttt tccatcacat acggatcaaa atataaatag aaatcgatag gaaaacctag
    40141 tacttcgaaa gtaccattga gtagtgcttc attttccaaa taaaatttat attcaatatc
    40201 actatctttt tgatactcac taaggaaaaa gctgatgatt tgattgactt tttctttatt
    40261 tgaattgatc gttaagacag gatctccttg tctttccgta agagctggta tttctgggag
    40321 ttctggttcc cgattagcaa aaatacggct gcctaaaaat acggtcgaac caagtactac
    40381 cccaagaagg ataaggaagg cgaatttcca gtaatttgtt ttctttgttt tgtttgaaga
    40441 tacgtcagat cttgttttca tgtttattga tcatcctcct tctctaacca ttggttttgt
    40501 gtctcgatca gctgatctct cactgcattt gccatcaatt gataacctaa attatttgga
    40561 tggaaatgat cttcttcata gagcacatta tttttgatgc catttagatt ttctgtcgtg
    40621 ctagttgttt gatctgttcc ttcttctgtg acacctactt cctgattcaa tccttgataa
    40681 agcaaatcat tgattgggat aaagtagcag ttcggcattt tttttgtcac cgtttctgtt
    40741 cctttgttcc aattgttaac aattgtctgc atgtcagtga tctcaggaaa gttcaaataa
    40801 aatggattgt agatgcctaa aatgtaaata ggtgcggttt tattgaggga tcgaaattct
    40861 tccaatagtt ctgtgacatt ttcctgatag tttttcagtg gctttttaaa ggaattgacc
    40921 gttaacccga ataaattatt ttggataact ttcatcaaat cattaccgcc aacagtcagt
    40981 gtgataaaat cagctgtttt gatattgttt cgaatttctt cttttgtttt tactcgcttt
    41041 aagatctggt cacttcgttc accatttaca ccatagtttt ctgtttcaac cgcggtcaaa
    41101 ttataacggt cttttagatc atttgcgaca aggggaacga atcctccttg cttggtttga
    41161 tctcctactc cttcagtcag cgaatctccg atagctatgt agtgaagttt ttctttttta
    41221 tttggatctt ctctcacagt agttactggt ttcattagtg ggccagcttt cggtatagaa
    41281 gcagctaaac ctgcatagat gatgatcgca attaataatg ggaacaaaac gaggatcaaa
    41341 tgtcgtttcg ttaatttcat tttttcacct cttgtattcg attggttcta atgttatcaa
    41401 aaattttatc aaaaaggtag tctttatgaa aaaagaaaac aattattttc ctaattttat
    41461 aaagaaaaag agaataagct gcaagccttt caggctgagg gcttatcctc ttttcgacta
    41521 atctacataa tacatgattg caaacgcgtc tttaccagta tgcgtagcga taaccggatt
    41581 cgtatgcagt acagggatat gcatgtccgg gaataattct tgcaaaccct ctttgaattc
    41641 ttgcgccaat tcttttgcat ccgcatgtga aataccgatt tgtcgcacat ttggcaattc
    41701 tttcagttca tttttcaaat tattaaacca tttatcgaac gttttctttc ctcgcccctt
    41761 agttacaggg attaattcag aatgggcgaa attcatcacc acgcgtatgt tcaaaacact
    41821 agacagcaaa ccagttgttc ggctgattct tccacctttt accaagttat ctagtgtgga
    41881 tattccgatg tataattttg tattgtcttg tacttttttg acttcttcaa ggattttctc
    41941 tactgtttta ccttcttttg ctaattcagc tgctcgaatt acctgaaaag ataatccttg
    42001 gtcagtggta tcactgtcga taaccgttac tttcgttttt gttagattac tggcttgtct
    42061 tgctgcttct actgttccac tcaagccttt tgtcatatgg atcgaaatca cttcactgcc
    42121 gtcttctcct agctcatcat acatcgagat gaattctcca attggtggtt ggcttgtttt
    42181 gggcagattt tttgctttcg ccatcatttc cataaatctt tcgccgtcta gttggtcatc
    42241 atcgggatat actgttccgt cgatcatgat ggatagcggc atgacacgaa tgcctaaagc
    42301 atcgcgtaca tttttttcca tcgtacatga agaatctgtt acgattttta catttgtcat
    42361 tattttctct ctctctttct ttaacaagat gccttattca tgttaaacta catgtaagac
    42421 aatttctatt tttagtataa cagacaaata atgatttttc attaattaca cctagtaaag
    42481 gagccgtttt catggaaaaa acgaaattta atcgaacgta tgaaatatta aatgaagtgt
    42541 ttaatgcagt cacccatgga attgggactg gattgagtat cgcgggacta gtggttcttt
    42601 tagtaaaagg tgcccacttg cattcctccc tccatattgt ctcttatgct atctatggat
    42661 cgatgatgat tcttttattt ttaacgtcta ctttgtttca ttcattgatt tttacaaaag
    42721 caaaaaaggt gttccaagta ttcgatcata gttcgatttt tcttttgatt gcaggaagtt
    42781 atactccttt ttgtttgctg agtatccgag gatggctagg ttggacttta tttgtattaa
    42841 tctggttgtt tgcgattctt ggcattatat ataaagcgtt gacgttgcat aaacgggaga
    42901 gagtatcaaa agtatctacg atcatctata tttttatggg gtggctctgt gtcgtggctg
    42961 ctgttcctct ttatcactct cttggaatga ctggtgttat tttgatggta gcaggcgggc
    43021 tttcttacac gataggcgca ttcttttata gcttgaattc tatccgctat atgcatgttg
    43081 tttggcattt gtttgttatg ttaggtgcag gttttatgta cttctctgtt ttattcttta
    43141 cttaataaaa aaaataaagc tatggatggg acagctctaa ataacagcta gtctatccat
    43201 agctttttta tatcgtcaat tcgcctgtcc cgctttttac aggattctca ttacgctgat
    43261 acgtttcaaa ttgatgcgga taacgatttt tttcatccat ttctccttga ctagtattga
    43321 tcagtgacca atcagaccag tcaacaggtg gaaaataagc atctccatcg aatacatgat
    43381 ggatgactgt acggtataat atcgtacaaa atggcagaaa ttcttgataa acagctgacc
    43441 ctccagcgat gaaaaagata ccgtctgttt ctttttccag agcaagcacg tcttccacac
    43501 tatgcatgac atgcacgcct tctgctttat aggaagaatc tctcgtcaaa acaatcgtct
    43561 gacgattcgg caacggacgg ctacccatcc cttcgaatgt ttttcgtccc atcactagtg
    43621 tatttgcttc tgtcatttgt ttgaagaatt tcagatcatt tggcaaacgc caaggaagac
    43681 ggtcttcttt tccaatcaat ccgttttcat cctgtgccca aattgcagct aacattttct
    43741 catcctttct gattctaaac agcgatagga gctttgatcg ccggatgtgg gtcatatcct
    43801 tctaattgga tatcttccat ctcaaaatca aatactgact gcttttcctg attcaaaact
    43861 aaagtcggaa atgcgcgtat ttctcggctc aattgttctt tcatttgttc gatatgattg
    43921 atataaagat gagcatctcc taatgtgtga acaaattcgc caaccgctaa acctgtttca
    43981 tgtgcaatta ggtgagtcag caacgcataa cttgcaatgt tgaacggaac gcctagaaac
    44041 acatccccac tgcgttgata caattggcag cttagccgtc cctcttgaac ataaaattga
    44101 aacatcgtat gacaaggagg caaagccatg ctaggaacat cttccggatt ccatgcagaa
    44161 acgatcaatc ttctagaatc aggagtcttt ttgattgctt cgatcacttg tttgatttga
    44221 tcgataaagc caccgtcttt tgtttcccag tgacgccatt gatagccgta gatgtgtcca
    44281 agatcgccgt atgtttcagc aaaggaatca tcggttagga tcttctcgca aaaaagcttg
    44341 ctctgctttt tatattcttc attgaattca ggatctgtca aacatctatg gccaaaatca
    44401 gtcatatctg gtccattata gtctggactt ttgatgtagc gttcaaatgc ccattcatcc
    44461 caaatatggt ttttattttg taatagatag cggatattcg tatctccttt taaaaaccaa
    44521 agcaattcgc ttttgatcaa tccaaaaggt actcgtttcg ttgttaacaa aggaaaccct
    44581 ttttgcagat caaaccgcat ttggtatccg aataagctta atgtgcctgt gcctgttctg
    44641 tcttctttac gatgcccttc ttccaacaat tttcttccta agtcaagata cgcttgttcc
    44701 atcctttttc ctcctaatct tctacccatt cactcaagta ttcccaccgt gccatttttt
    44761 cttcaagttg tgcttctgtt tcagatacgt cgttttgcaa ttcttgtaat cgagtgaaat
    44821 tatctccttg atgattcatc tcttcttgga gcaatgagat tttttcttca agttctgcaa
    44881 tctcatcttc aatcgtttcc cattcttttt gttccatgta agataatttc ttcttttcag
    44941 caggttcctt gctctgtatt tttggtttcg ctttttcagc aggctgctgg gtctcttttt
    45001 gcttcagtaa ataactggac atgtttccgt aataaagttc ttgttttccg tttccttgga
    45061 atactaataa cttatctgct accttgtcca agaaataacg gtcatgggaa acagtgatca
    45121 cagcacctgg aaaacttcta aaataatctt ccaaaatcgt caatgttgct atgtccaaat
    45181 cattggtcgg ttcatccaat aataaaacat taggttgctg aatcagcagt ttcaacaaaa
    45241 ataaccggcg tttttctccg cctgaaagtt tgcctatcag tgtcccatgc ataaatcttg
    45301 gaaataaaaa tcgttcaagt aattctgcta ctccgatttg actgccatct gctgtttttg
    45361 cttcttcagc agcttcttgt aagtaagcaa tcatccgttt atctggtgcc atttcttcat
    45421 tttcttgtgt ataatacgca agatgaaccg tttcaccgat agacattgta ccgctatcca
    45481 ggggaattct gccagctaga atattaagca atgtcgattt tcccgcacca tttttaccag
    45541 taatacctag tctttcccga gattgaatca ataaatctaa atgttctagc agtgtttggt
    45601 catttatacg gtaactgcca tctttgattt ccagtacttt tttccctaaa cgttgtgtcg
    45661 ctaggttcag ctctaaatca tcttcttggt ttacttggta caagttctcc tttagatctt
    45721 cgaaacggtt gatccgtgct tgctgcttgg tgccacgtgc ctgtacgcca gccctcatcc
    45781 aagctaattc ctgtttataa agccgcttgc gcttttcttc ttgttctact tctacacgat
    45841 cacgttctgc tttttctaag acgtaagctt cataatttcc tttatactcg tataattttc
    45901 caaatgaaag ttcgaagatt cggtttgcta ctctatctag aaaataacga tcgtgggtaa
    45961 ccatcaatag tgcgccacgg tattgtttca aatagttttc caaccactcg atcgcttcat
    46021 aatctaaatg gtttgttggt tcgtctagta atagtaaatc gggttcatca atcaacactt
    46081 gcgccagact aactcgtttt ttctgaccac ctgataaagt actgattttt ttatctaatt
    46141 cactgattcc taatttttgc aaaataatct ttgcgttggt atctgtagtc caagcatctt
    46201 ctttattcat ttgttcttct gctttagcat aacgtttttg cacatcaggg tcttctccgt
    46261 tttcccctag ttcgatcaat gcttcctcat atgctcgaac agtttggatc aaggggctgt
    46321 ttccttgaaa gactgcttgg agaacagtta attcatcgga aaattcagct gcttgagaaa
    46381 gataaccaat acggtaatca gttggataaa acactgtttg ccgatcaccg tcgctgctat
    46441 cgataccggc aataatattt aagagacttg ttttacccgt cccgttcgtc ccaatcaacc
    46501 cgatacggtc tttttcgtgg accaaaaaag aaatctggtc aaacaaatct ttttcaccat
    46561 aagttttttt gaggtcagtt acttttaatt ctttcaacat gttccctcct tatcttggat
    46621 attgtatcat aaaaatagtg aacttcagga aaattttttt ggtatttgtt ctttttgtgt
    46681 tctcaaaaaa agaacaggac cgattcgatc ctgttctgat atgttttgtt ttttataaaa
    46741 cttgaaaatt tttacctttt tattttattg gactcgtttt ttttgacttt gctcgaaacg
    46801 caaatcggaa agaaaagtta tcagatggtt tgttcatcgt ataagattct aacaaattga
    46861 tattcctgcg atagctgttc gcagaaaacg tcgtgatttt ttcagcaact tcgtattcat
    46921 caatgatttt tctttcaaga taataacctc ttagcatttt tttcgtaaac agactatctt
    46981 gctggatcat ccacgaagaa atcaaattcc ccgagttcat ttttattcct tcaagtttgc
    47041 gttgctggat aaaaaattga accatcgttt catcataaat atcagtcaag ttatctaaac
    47101 tttcaataat caactgagta tttttttgat atgtctctct gatttcttgg atatcttcat
    47161 cactaataaa tgtctgacga tcagcatatc ttctttgcca aaacattttc ggatggagaa
    47221 cgattcgcat taacctacgg acaacaatga accaaaagcc gataaaagac aaaatctggc
    47281 ttgtgataga atgttcgaat cgagaaagaa aacgggagta aaaacgataa ccattagaag
    47341 aaagcttgcc cgaacgataa ctttcatcca atccatcccg ttcgatcgac aaaatcagtg
    47401 cttgtatttc ttgtacttct tgcttttcat catcggttaa atcttccaag tacagatcac
    47461 gtatccgttc ttgataattt tctatcactg cttcagtggc taaaaattct tttgtattac
    47521 gatcaatttc ctttatttct tttcgcaaaa cttcaacgac ctcttctaaa atcatcaaag
    47581 cattcaaatc cgtgcttttc gcttcctctg tttccgtcag cataggcaag acaatgatcc
    47641 ctatgaccaa agtcactaag atgacacccg ccgtcagaaa caaaagcaaa gaccgctggt
    47701 aaaacatcat attattgatt gaaaatggca aaataaaaat cgtagccaaa ctgactgttc
    47761 cctttacacc gccaaatgtt aagatcaaga tctcgttcaa ctgttttttg aactttttac
    47821 tgccatcttt gaatacataa aaaagactga tagaaatgaa tctgatcaca aaaagcatga
    47881 cagagattaa tacgataatc gctaataaaa gaccgtttgg atataaaccg tctccccata
    47941 ctggcgagaa cacttgcgtc aattcgattc ctaaaaatat aaatactaat gcattcagtg
    48001 taaaagcaat agttgtccat gtactgtggg acaagctcga caattctgca tcaaacacag
    48061 agattttcct aaaaccagaa gcttggagaa tacctgctgc cactgcagca atgattccag
    48121 atacatcaaa tgcctctgcc aatacatagg ctagaaaagg aagcaaaagt tcgatcagca
    48181 gataccctgt gacatcttgt gctgatgctt tttcgatcaa atgaattatt ttttgtttga
    48241 accagacaag caaaaagccg atcaaagctc ccccaatact agaaactact agactcatcc
    48301 ccgcgtttac tgctgaaaaa gatccggtga tcagcgcacc aagagcaaac tggaaggcag
    48361 taacaccaga tgcatcattc aaaagacctt ctccttccaa aatatgcatc gcctttttag
    48421 ggatattcac tcgtccagaa agagaactga ctgctaccgc atctgtcgga cctaacgctg
    48481 ctccaaaagc aaagcaggca gcaagtggga cacttggcaa caggaatgat aacgtcgcac
    48541 ctaccccgac aagggtcaaa atgactcctc caaatgccaa gaacaaaatt gtcccgaaat
    48601 ttttgagaat cgaagaaata tctgcttttt ctccttctct aaataacaat ggtgcaataa
    48661 tcataactaa gaacaattca ggttcaaaat cgattgattg tccccactct gtcagaccta
    48721 ataccactcc aaggaaaatt tggatcagtg gagctggaat cattggcaaa atgcggctag
    48781 ctatgttcga taaggtgatc gttactgcaa atacaataac taactcagca aattccatcg
    48841 tccatcccct ttatttttta ttttgtgtaa gcatttttaa aaattgttct tttttcttta
    48901 aaattttggt ttcgactact tcaacctgtg tttggttccc ctctaattca cactgtacac
    48961 gttttgactc caaacgaagc agatcatgtt tcagacgttc caactgacga tacatcaata
    49021 tatttctttt acgcaactta caaagtattt gacggcggtc atccatcgaa agggcgccat
    49081 agcgatcatg caacagtttt ttttgttgtt caaaactctc tctcattttc cagatctcct
    49141 atacacaaac ttccatcatt atagcaaagt tctttttgga tgaaaaatat ttcattgttt
    49201 catcgtttat tgctagttag aaatttccat aaatttttca acgcctcatt ctattccaaa
    49261 tagtatcaga tatcaagtaa aacttaagaa agataaaagt ttctgacaaa aggatgttta
    49321 ctttcccatt cctgcctttc ttgttctttt atcacaacaa aaaaatgggg ctgcgacaaa
    49381 aatcttgtca cagtccctta ttctgaataa acggtgtgga agagacagcc tatcagtagt
    49441 aataagacac atctctttca cagcctcatt tttgcgtaaa aatcgattta ttcgaaaaat
    49501 tccccgtata catcttctac acgttgatct ggattgatca cgatatacaa gttacctgtt
    49561 ttttcgctga tttttgttga ttgatattga ttgtctaatc cttctgttcc tttatctaga
    49621 tatgggaagt acacaagatc aggtgtgcca tttcggtaaa ggatttccat tcgctcgata
    49681 tcttcgtatt tcaatgcgcg attaaacatg cccatttcca gtccgcctag gttgatatca
    49741 tgtgtcgcaa ctgcgtcccc ttcatggaag atttcgattt taaatccagc acacggatgg
    49801 atttcaacaa attcactgcc attgattcgt ccaaaacttg ttgtgatttg tttgatccaa
    49861 agatcgccga tatgacggcg gttgatcgtc caggtttctc catttctaaa ataaaatttt
    49921 agcgctgtca tttctttact cattaaaaag actcctttat gatgtaattt attcttgtac
    49981 tacctcagtt tagcacaaat ttatgtaagt ggtcacatct tatgtttttt cttctaatga
    50041 cgtatctgcc cactttaagt agccaacaaa gctgagcaga tacagtctag tgttttttaa
    50101 atcaattttt ctttcgataa aaaatccaaa acctgttttt tatcattttt tattctttgt
    50161 tctacgacgg ctttttcagc taattgcaaa gcttcactaa cccacttacc aggttttttt
    50221 ccagtttctt ctagcaagat tttaccatct actgctaatt cgcttaatga cttgataggt
    50281 aaacgatcat aggattctcc tacggcatcc atctgaggtg tttcgtcaaa atagatcatc
    50341 aattcttcta cttgcagtgc atgttttttg cctaagaagt acaattcgaa tggttccaaa
    50401 gctccagttt ctcgttgtcg tagtccttgg acaagtgctt gaacgtcttt aatcatttgg
    50461 ttagaacact tccacgattt caaaaaggaa cgaatctcat tttcttttag atccagttgg
    50521 ctgatcaata atgcccatgc ttggctctct tctttcaatg ggacggatgg taattcagca
    50581 aaacgtaaaa gtgcttcgcc ttcttctttc aaaccgggac agcaaatgta gcactctgtt
    50641 tcaataaatg catggattcc cgcggcccgt tcatttccca agagcatctt gatgaattcc
    50701 acatttatac gttctacaga tattttacct aatagctcgt ggttttcttt gatggcgtag
    50761 aaggtttctt cctctaattt gaaccctagt tgactgacaa aacgcaaccc gcgcatcatt
    50821 cgtaaggcat cttcatggaa ccgctcatga ggattgccta cagctcgcaa gatctgttct
    50881 tctagatctt gcagaccatc gaacaaatcg atgatttctc catcttcttt caaagcaaac
    50941 gcattgatcg taaaatctct tcttttcaaa tcttctgcca acgaacgaac gaattcgaca
    51001 tgatctggtc tacggtagtc ctgataagtt gattccgtcc gaaatgtggt gatctcatat
    51061 tgttcatcat tttctaatac caacacagtt ccatgttcga tcccaatatc tatcgttttt
    51121 ggaaaaagct gtttgatttc ttctggaaat gcgcttgttg cgatatccac atcatggatt
    51181 ggctggttca gcaagatatc acgaacactt ccacctacaa aataagcttc atatcctgca
    51241 ttctctaatt ttttcagaac tggaattgcc ttttggtatt ccagaggtaa ttgttttaat
    51301 ttcatttttc caacctttcc tgcgtgtttc ttgtcctgag ataaagactc agttgcatgg
    51361 tgactgtttg acaagtccgt ttcagagtct tcaaggccag gaccacttca tcttgtttac
    51421 tcgtccttac gctcaaagcg attatggtct ctttgattga atttttccat gtttttttca
    51481 aaaacttcag ttaagtcgat attcaatgaa ttagccatga tcatcgtaac gaataaaaca
    51541 tcaccaagtt cttctgctac cgagttaact ggttcagaag cctttttgct tttttctcca
    51601 taagtgtggt tgacttctct tgccaattca cctacttctt cagttaatcg agccatctga
    51661 gctaatggag agaaataccc caccttgaat tgctggatat attcatccac ttcctgctgc
    51721 atactgtata aagaacgttt tttatccatc tttttccctc caaaactatc ttaccaaaac
    51781 tttaacgcaa atcctagata agagcaattg agaaaatcga atttccatgc tacatttagg
    51841 aaggaatttc taaatggaaa ttctgtgaaa atacataatt gaaagctgga attcatatgg
    51901 tcaaacgaaa aacagtaaaa gaagttcttt ttatcattat aggaacaagt atttatgcat
    51961 ttggtcttgt ttacttgaac atcgctaatc atttagcaga agggggagtc tcagggatca
    52021 ccttgatctt aagagcttta tttggtattg atccagctta tacgacttta ttaatcaaca
    52081 tcccattgat tttactagga ggaaagatcc tcggtaaacg ttccttggcc tatactgttc
    52141 taggtacact ttctttgtca ttctttttat ggatctggca aagggttcca ctagaaatca
    52201 atttacagca tgatctactg atcgtttctc tacttgcggg tttagttgca ggtgtaggaa
    52261 gcgggatcgt ctaccgcatg ggtggaacaa caggcggcag tgatattatc gctcggattt
    52321 tagaacaaaa ttacggcatc actatggggc gttccctatt ggcatttgat atcatcgtct
    52381 tacttgcttc attaacctat atcgacttga aacgtatgat gtatacgttg attgtttcgt
    52441 atgttttcag tcgtgtgatt gattcgatct tagatggcgg atattctgca aaaggtctac
    52501 ttgtcgtctc caacaaaagc gaagagattg caccactact gatgaccggt ctgcaaagag
    52561 gtgtgacttt cctttctgga gaaggcgcat tctctggtaa ttcaaaaaaa atcatctata
    52621 tggtcgttag ctctagtgaa ttaactgaag ctaagaggat cgtccacgag attgacgaaa
    52681 aagctttcct ttcaatcatc aatgtgcatg aagtggaagg gcaaggcttt acttatttga
    52741 agccacagac aagattactg aaaaaactat aagaaaaaag acaaagatag gctatgagga
    52801 aagattccac atccagctga cctttgtctt ttttagtact atactatttt taactaattg
    52861 ttatgttcac ttacccggtt acttttcttt tgtgtctgca aaaggatctt tcggttaact
    52921 tcatcagata atttcaaaaa agcatcccta tctccatctt ctagtgcttg atcgatttga
    52981 gcataaagca tttttagttt tgcctcttct tctttttctt gaaaatatcg ttcaattaat
    53041 tcagtatctt cttcactgac tgaagaatcc caagaagaaa atggattgtc ttctaagata
    53101 gatagatatt ctcttgtctg ccaagcattt tcgaatatac attccacata taaaggcttt
    53161 ttccaattca aacggatttc atgaaaaatc tggtctgtgt ccgtaaattc ttttccctgc
    53221 aaaaacaatt tcatgggttc cccttcaaca gatagatcac tgatctgcag tcctcgatcc
    53281 gcaaaattcg ccccttcaac aaagtggaca ttgcttaaaa tcgcttcatg attggcaaga
    53341 taattcaaga tccaaaaaac ttctcttcgg ctaaaagata catgattgac catccacgtt
    53401 aaaaaattct tcttttcccg tacatcaatt agcatttttc tcacctctct gttaggtcat
    53461 ctaataatga cagtgcctcc atatctcctg gttcaagtgt taaataataa gtcaataatt
    53521 cttgcgctct tttcagctgc ccttcttctc ttaagaaaaa ggcatactct ttcaagaaat
    53581 ctggttcatg tgatagttca tgataagcct gttcatagtg tacagctgca acggtaaagt
    53641 cttctaattc attataagca tgggctaggt tccattctgc ataaggatta gcagtttctt
    53701 ccatctgatt gatcacttgt atcacatctt catatctttc atcatccaag taaagattac
    53761 ttaaagtaag taatgtttca tcttgtttgt ctcctacttc taaagctttc agtaaaagtt
    53821 cttctgctcg ctgtctatca tgcagacgga aagcattttc cgaggccagg tgataaagtt
    53881 cgacttggta aggattttct ttgattcctt cttctaacac tgtttgtgct tcttcaatca
    53941 tttcttcttc ttgaagagct tgtcctagat atagatataa cgattgataa tgcggattaa
    54001 cttctcgaag ttcttccaga tagttgattg cctgctgatt ttctttcaat tgaagattcg
    54061 taaaagctag ctggaacagt cgatcatctg tcctttcttc tttcagtgct tcttctaaag
    54121 gtacgatagc tgcttcgaat tctccttgca tacttaagga ttgtcctaaa cgttcctgca
    54181 tcgagattcc tgaaatttca tttatcccgc ttgccaacaa tgtttcataa accactgctg
    54241 cctctttgaa acgatcaact gaaaaataca attcagctag cgcaaactgg atcaatggtt
    54301 catcaggaag caacccagct gcttctttca attttgcttc actcacttca gggattccga
    54361 taacttggta caaatcagct atcgccaata agctttgcgg atagtaatca ctttgcttat
    54421 ccactttttc caggtattca aaagctaagt cgatctcatt atcttcgatt gcaatttctg
    54481 ctaacgggat gttcagtccg tcattttctg ggtattggtt gatcagttgt tcaaaaatct
    54541 gttttgcttc gatcaaaaag ccaatagcca gcaattcttc gcctagttca gctaatatat
    54601 ctgctgggtc ttcttttaat gctttttcta aaaagatttg tgcttgtgac aagtcttcat
    54661 ctgataaagc ttgcaacatt ttttcgctat ttgtattcat ttcaattccc tcttatctca
    54721 ctgacgattt gttgaaatac cgtcattgtt ctcaataatt ctttttcgtt tggatgttgg
    54781 tatccgtcta ttatacctat tttttctaaa acaagtattt ttttgctttt ttctgccaaa
    54841 aaaagaactt tttccaaata ctccatcttt gaaatctgtt ccagccagtg gattggatag
    54901 ccaagcaagt ccagccacac aaaaaagttt tttagttgga aatcaaaagg atatgcttgg
    54961 agattccaca cgaaatgaaa catcattcct aaaaattttt tcatactagc agaaagtaga
    55021 tggccatttt cggtcagata aaaagcttgt tcaaacagtt taccataatt tgacaggttt
    55081 tctgcctcta attgataaaa atagatcatt tcttcgatca tcccggcaaa cggccgagtc
    55141 gttaattgct ttttatttgg atagttttta tacagatttt gcaaaaaacg atgatcacaa
    55201 atcattccgc aacagataaa agtcaaaaag tcaatctgct ttccgtccac ctgccgctcg
    55261 ctgattgttt gatcataaac gatgcgttct ggcagattga tggtttgcag cactacacga
    55321 tttggtagct gcaaaatcgt ccgctgcggt gttaatgctc ggcttagaga acggatcgat
    55381 acagggatca tccatagtga tgaatcaagg gttgtatgtt tttgaaaaaa acctgccaga
    55441 tccatgatcc cttcatttcc aaatccgatg ataaacatgg attgattttc gggatagcgt
    55501 ttgacgaaat caagcaggtt ctttaattct gttaaatgat tgcattgagt gttggcacaa
    55561 atataccagt cattgtttgc ttgatttgca aataacgggt tgatcttatc tgcaaacaaa
    55621 tcataatagc gctgattcgt taaaaataag attggctgat caatggaaag ttctcttagc
    55681 tgactgacta gcgtttctcc gtaaaacact ttcgtcaacg catcttctac ttgataatca
    55741 agttccatac gcatccctcc tcttcttatt tttattttac cattagtaag caaattctag
    55801 aaaagataag agcaaagctc acctcataac ttgcaaataa ataaaaatta aataaaatac
    55861 atgtgttttc tatgtatgac atacggattc ggaatctctg tctcttattt attccttttc
    55921 tgctaggaaa taaaaagaaa agcacttgta gccctagagc cacaagcgct ttttcaactg
    55981 tcttatttaa cagcgtcttt taatgcttta cctggtttga atgcaggtac tttgctagct
    56041 ggaatttcga tttcttcacc agtttgtggg ttacgacctt tgcgagctgc gcgttcgcga
    56101 acttcaaagt taccaaaacc gattaattga actttttcgc cgttagctaa agcatcttga
    56161 atagttgaaa atacagcatc tactgctgca gttgcatctt ttttagttaa atcagtagct
    56221 gaagcaactt tttcgattaa ttctgctttg tttgccatgg atagattcac ctcctccaca
    56281 agagatatga tagaaatatc atttagttaa aactttctga gtggtccaga agtttaaaaa
    56341 gaaatattga ttaacttcaa tattctgtta tcacgatatc atatgaaccg cttaatagca
    56401 aggtttttac caattttcac atgctttttt tagtcataga aacgtaacaa acgaaactgt
    56461 ctttttcttt tgaaaaaaaa acgtcgatat ggtaaaatgc ctattttctc ctacgaggga
    56521 taattcggat ggacgtccct tcaaaagtaa atgcttttct gatttgattt tctaagaaac
    56581 gttcataaga aaaatgcatc aattcctctt cattgacaaa aacgacaaat gtaggtggtt
    56641 tgacagcgac ttgtgtagca taaaaaatct tcaggcgttt tcctttatct gttggtgttg
    56701 gattgattgc tacagcatcc ataatgatat cattcaacac agccgaagag atacgaagat
    56761 tctggttcat acttacttgt tcgatcattt caggcagacg ttccaaccgt tgtttagtca
    56821 ctgcagaaac aaaaacgatt ggagcgtaat caagataccg gaattcatca cgaatctctt
    56881 gttcgaaatc acgcattgta ttggtttctt tctttacaag atcccattta ttcacgacga
    56941 taataattcc acgaccagct tcatgggcgt aacccgctac tcgcttgtct tgttcccgaa
    57001 tcccttcttc agcattcagt accatcaaaa cgatatcaga acggtcaatg gcacgcattg
    57061 cacgcatcac gctgtatttt tcagtatttt cgtatacttt tccgcgttta cgcataccag
    57121 ctgtatctat cattaagaat ttctgaccac tttcagaaac gaagtgggtg tcgattgcgt
    57181 ctcgggtcgt tccttcaa
//
LOCUS       DACEUZ010000004        56096 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.004, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000004 DACEUZ010000000
VERSION     DACEUZ010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 56096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56096
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.004"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(855..1385)
                     /locus_tag="IXG44_000204"
     CDS             complement(855..1385)
                     /locus_tag="IXG44_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferase"
                     /protein_id="HAQ1656949.1"
                     /translation="MRLGLIIKNILNLPKTIYFNYKFLHISDAWRLPILVHRKVRITR
                     KSRGKIEIVLQSGKIVYLGFPVLNTAGNEETIISIEGILRIQGPAFIAQGAHIDIRKN
                     AVLDIGSKFNSTGKMSIICFKEIQFKDGCTVSWDTLFMDTDSHTIFELNTHRILNKNN
                     KIVIGKHCWIGAKSVI"
     gene            complement(1382..1999)
                     /locus_tag="IXG44_000205"
     CDS             complement(1382..1999)
                     /locus_tag="IXG44_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAQ1656950.1"
                     /translation="MIIVIAEIITDILYKLYEPFGFAVLLTILGLVYFMYATDGIAAG
                     EGYKRILMELYLHLKKDRKYRKVCALIFYTAMILFTTLINRTIWLNPLSDVMGDWWIY
                     KYNTTTGTKELSTACLENIILFIPYVYMVMSIKYENIKKPKIWSSIVYGGKIAFYFSA
                     IIEFLQLFLRLGTFQISDFVYNTLGGILGVILYKLILITKRDGER"
     gene            complement(2581..2802)
                     /locus_tag="IXG44_000206"
     CDS             complement(2581..2802)
                     /locus_tag="IXG44_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferase"
                     /protein_id="HAQ1656951.1"
                     /translation="MGSSERIKIGNNVFIGMKSRILKGVHIGNNVVIGANSLVNKDVS
                     DDCVVAGNLAKVIMSLDEYYKKINNMKNP"
     gene            complement(2980..4512)
                     /locus_tag="IXG44_000207"
     CDS             complement(2980..4512)
                     /locus_tag="IXG44_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ1656952.1"
                     /translation="MIINRKQNAKRNIITGTIFKLYQIIIPFFMRTLMIYQMGMEYAG
                     LNNLFTSIFQMLNLAELGIGAALTVSMYKPIAEDNRKEICALLNAYKKYFSIIGWIVF
                     TLGLLCIPFLRFLVKGSIPGNLNMTVLYLMYLINTVLSYWLFSYRKSLIYAHQRNDVI
                     NVSMLITFTFQFVMQAYVLIFLKNYYLYLLASILGQIVLNLMCAVVSQKMYPDYTPVG
                     ELEKETIDDIIKKVKGLVTNKVGGTILRSSDSIIISSFLGLSVLAIYQNYYFIISAIM
                     SIFAIIYESCIAGIGNSLIVESQEKNYIDFCDITTIIGWFLAISCACFITLFQPFMII
                     WVGSDNLMSISFVIFMIVHFFLYEIDQLIGLYKDAAGIWYADRFRPLITAMINIVLNI
                     ILVNWFGLYGVLAATIVSVLLVDLPWLLYNVFQNIFKFESAGKYVWKLISLCVIAFVA
                     SYLSYFACSYVNIGNFKVISLVINLVISVVISILVILLFNIRNKKFKYILKVLTSVLV
                     KR"
     gene            complement(4648..5733)
                     /locus_tag="IXG44_000208"
     CDS             complement(4648..5733)
                     /locus_tag="IXG44_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ1656953.1"
                     /translation="MKKICFISGVISRSGGTERVGSIIANSLSDLGYDVTILSFWNSG
                     EPYFGLDKTIKVDYMLNPKTEGKLYRTYLYPVWKLHKYICKNEFDIVIDIDTLLSLYT
                     TRAIKGTKCKLISWEHFNYWTMEMLKEKKRFKAKKLIKKYAKRLVVLTEEDRQKHIDE
                     YSLSEDFVITMPNPCISNIEYEYHYDKKRFLAVGRLVEEKNFADMISAWKIVEKKVDD
                     WDLIIVGKGELEKDLRQQIKDLNLQRVTIAGHSDHVEEYYKSASCYVLSSVYEGFPMV
                     ILEAQSYGLPVISYDCKTGPRDLVHHNFNGMLVEDKNIDQLAKSMIMFTKNTDLAMRM
                     SLNAYNNVQKFNLKEITKQWVALIENV"
     gene            complement(5748..6467)
                     /locus_tag="IXG44_000209"
     CDS             complement(5748..6467)
                     /locus_tag="IXG44_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAQ1656954.1"
                     /translation="MIPKKIHYCWFGGNPLPKKDRKCIESWKKYCPDYEIIEWNESNY
                     DLNKNRYMADAYKERKWGFVPDYARFDIIYNHGGFYLDTDVELIKSLDILRENHAYMG
                     FEGEIWVNGGIGFGAEEGHSVIKGLRDIYDSLSFYQNDGTLNLKPSPYYITDYLESIG
                     LVRDNSEQIINGLHIYPTDYFAAKDYDTGKIYKTSNTISIHQYNASWKTPKQKFVYRL
                     KSTIGQKKFNRLLALKKFLHL"
     gene            complement(6464..7774)
                     /locus_tag="IXG44_000210"
     CDS             complement(6464..7774)
                     /locus_tag="IXG44_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAQ1656955.1"
                     /translation="MRISIKRWSGADWTLFTLAFFAISIVLVYTFNAPRAILYLGDII
                     NIFVLLNALSFRRRKGIYASKNEMLIVLFLLVSICSSVINLENIGLIIWGIRNNTRMF
                     IVYINCATYLTIKDYYKVFAIIEKLFWISLPLCLIERFMVTYPTGTIVGDMIGGVFWN
                     YSGCNMPLNLILCVTVTVVTIGYFKGEKNFIHFILCVCAALFMSALAELKVFIPELFI
                     IFVAIVFTLKVDFKVVIKILSCVILVSIVLSFFITIFITLNDNGQSYADNFTIQGLLE
                     YASRDSGYDGVGDLNRITGISRVVDEIFNNNFRLVLMGVGIGNAEFTNFFVSEFYKSF
                     GNLHYQYFHHIWLFIEVGILGVSIFLFFIIKTFFNAKRSFMKDKDASLVKVIAIMSVF
                     FFVYNTTLRGETTGPLIYMILAMPEVVKRKEHYYSEKNTKGEIL"
     gene            complement(7801..8391)
                     /locus_tag="IXG44_000211"
     CDS             complement(7801..8391)
                     /locus_tag="IXG44_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein"
                     /protein_id="HAQ1656956.1"
                     /translation="MEISSKEYLKAVLRMEKNLYIESKGQHTKLLKIMKSHPDYNAWR
                     YTRRMRITSYYYSKRKKNIIYAIMYIVSCRRMNSLGRKCGIECGENVFDEGVRFYHTQ
                     GIVINGNAIIGKNCYLYGNNCIGNDGIDPKCPVIGNNVRVCVGAKIIGNVKIANNVVV
                     AAGAIVIKDCLENGAILAGVPAKIIGYVNLTKVFLE"
     gene            complement(8709..9770)
                     /locus_tag="IXG44_000212"
     CDS             complement(8709..9770)
                     /locus_tag="IXG44_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ1656957.1"
                     /translation="MNSSDLISVIIPVYNVEKYVGSCLESVCNQTYKNLDIIIVDDGS
                     KDSSNKICEDYALKDSRIRIIHKNNAGLGLARNTGLEHVKGKYVAFIDSDDFVAANYI
                     EAMLNGIKRSNADTCYAGYYEYYDDNNIISKPARYDSTIFTNNAIIEKVLLGMIGTEP
                     SYKVDFILPMSVWHALYSSEIIQQHNIKFPSERDYISEDIIFHISYLQHSKITSFIKD
                     CIYYYRCNNNNSLTHNFRDNEFDCNIELVKKIEGDLSYILENEIFVNKTRRLLLGRCR
                     TCIQKAVRYASFNKEFDLNSYLSKILMNDELIACIHSYPYKQNPIKQRIFNYFLLNQS
                     IIGVKTLAYLSGMGRNRRF"
     gene            complement(10338..11405)
                     /locus_tag="IXG44_000213"
     CDS             complement(10338..11405)
                     /locus_tag="IXG44_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ1656958.1"
                     /translation="MQRVLMIGNDSSVKGGITSIITQMLNFNWEKKNIEMTFIPSYKG
                     GNAIFKSLYFMYAFAKIIGISLLSKPNIIHMHMSYRGSFSRKYVLNKWFRLLNIPVII
                     HLHGSEFKKWYDSVSVKKQNQIRLLLRESDSTIVLGEKWKKIIKEIEPNCKTVVVENS
                     IVVPEYTIKWNSKMCRFLFMGVLIKRKGVHDLVEAVKNIAEQYPEKKFIFEIAGAGDE
                     EISLKELIEEYNLQSYFEFTGWVVGEKKEECYRRSNVMILPSYNEGLPISILEAISYG
                     MPVIATDVGDISSAVRDGENGYLFQPGDVSALTSAILKINDKDKYVKFSRCSKDLAIT
                     KFNDGNFFDRILNLYQKGSKE"
     gene            complement(11425..11904)
                     /locus_tag="IXG44_000214"
     CDS             complement(11425..11904)
                     /locus_tag="IXG44_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta(1,3)galactosyltransferase EpsH"
                     /protein_id="HAQ1656959.1"
                     /translation="MIFITLGSQKFQFDRLLKAIDDLIEKGVIIESTFAQSGYSDYKP
                     LNYSYKAFLNRDEFTETMQKAEMVITHGGTGAIIGAVKKGKRVIAVPRLAKYGEHVDD
                     HQLQLIRQFDEMNIIYPCYELENLEDAVKETRVRMFNKYVSNTETIINSIEVLIEKI"
     gene            complement(11921..12382)
                     /locus_tag="IXG44_000215"
     CDS             complement(11921..12382)
                     /locus_tag="IXG44_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ1656960.1"
                     /translation="MNKKICFAASSGGHYEQLMMLKPLMEKYESFVITEQTEYSAESK
                     GEKTYYMRQVNRKEKFFLCTILINLFKSIIIYIKEKPDLVICTGVLAMIPICLITKMM
                     GKKLIYIESFAKVTSPTQTGKLMYHFADQFYVQWETMLQFYPNAIYLGGIY"
     gene            complement(12396..13646)
                     /locus_tag="IXG44_000216"
     CDS             complement(12396..13646)
                     /locus_tag="IXG44_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HAQ1656961.1"
                     /translation="MKKKYNIAVAGTGYVGLSIATLLAQHHQVTAVDIIPEKIELINK
                     RKSPIQDEYIEKYLTEKELDLTATLDARSAYKSADFVVIATPTNYDSRTQHFDTSAVE
                     SVIEVVMDVNPDTIMVIKSTIPVGYTESVRETTGSKNIIFSPEFLRESKALYDNLYPS
                     RIIVGTDMTDERLLNAAHVFATLLQKSAIKENVDTLFMGFTEAEAVKLFANTYLALRV
                     SYFNELDTYAEMKGLNTQQIINGVCLDPRIGTHYNNPSFGYGGYCLPKDTKQLLANYA
                     DVPGNLIEAIVESNRTRKDFIADRVLQMAGYYNYAEYNQYDVNRENPVLIGIYRLTMK
                     SNSDNFRQSSIQGVMKRVKAKGATVVIYEPALKDGETFFGSKVINDLEQFKAMCDSII
                     ANRYDTCLDDIKEKVYTRDIFQRD"
     gene            complement(14024..15475)
                     /locus_tag="IXG44_000217"
     CDS             complement(14024..15475)
                     /locus_tag="IXG44_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAQ1656962.1"
                     /translation="MYKKSSRGWVKHIDFIILDLLCIQLAFYLSYVLRQVDWNPYVIP
                     MYQNMAVFIELADLLVIFMFEAYRGVLKRGYYIEFASSVKQAIMLVLLGSLYLISVQG
                     GNDYSRVVLFLMGGLYVVITYITRLLWKHYLKHKMKNEGNKSLIIVTSSSIAEVVIRN
                     VKEHNYKMYKINGVIIIDKSMIGEDVEGVTVVSDSKNAADYLCQGWVDEVFIILNKST
                     SCMKEFIERCSEMGLTVHLNLAKVSDSALGKQSIGRIGEYTVLTTSINVMTTQQAFIK
                     RTIDILAGIVGCIATGIIFIFIAPLIYINSPGPIFFAQERIGQNGKPFKMYKFRSMYM
                     DAEERKVELMKENKMSNNLMFKMEFDPRIIGNKILPDGTKKTGIGQFIRSTSLDEFPQ
                     FFNVLTGKMSLIGFRPCLKSEYEEYDYHHRARIAIKPGITGMWQVSGRSDITDFEEVA
                     KLDTEYIKNWSMGLDFKILFKTVKTVLQRGGSM"
     gene            complement(15796..16560)
                     /locus_tag="IXG44_000218"
     CDS             complement(15796..16560)
                     /locus_tag="IXG44_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HAQ1656963.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKTPVIVHPERNVHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPVYEEVKKKKFGLF"
     gene            complement(16588..17286)
                     /locus_tag="IXG44_000219"
     CDS             complement(16588..17286)
                     /locus_tag="IXG44_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HAQ1656964.1"
                     /translation="MARTQKQKAAQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVVYKTFQLNNAS
                     GLSTALSSSGSVADVIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILSEARQLFDV
                     VIFDMPPVVAVTDAQIMSLKTDGTLLVVRENMSRKESLTKAKELLEMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(17298..18077)
                     /locus_tag="IXG44_000220"
     CDS             complement(17298..18077)
                     /locus_tag="IXG44_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HAQ1656965.1"
                     /translation="MEETISLKEIFETLRKHLVIIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKDAISVNQAENSQMFSIQAISTNAVAAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLMTGLMLGMMVGVGLAFLLELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGTAPARPKATNDKTAPSRRSRSRV"
     gene            complement(18093..19037)
                     /locus_tag="IXG44_000221"
     CDS             complement(18093..19037)
                     /locus_tag="IXG44_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ1656966.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMNFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(19083..19862)
                     /locus_tag="IXG44_000222"
     CDS             complement(19083..19862)
                     /locus_tag="IXG44_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1656967.1"
                     /translation="MEKPFVKLLATVAVGTGAIVICLFGYHLNNQRQYHQRINYAESA
                     ITNQKDTVTSLSKEVDKLYSTKEKIFLNPEITEETVSNLSHKLSSIKLSADDYDIKES
                     ELPKEAAAIQEEKKAVLTQLEDAESKLKIQTAVNKLFTKNVSNWQQAVDDVIIKEKLA
                     SADVAHVRENMSFFKDSAWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSQIEQIRNEKIRAAYATKAQTVAQQMGYSNISY"
     gene            complement(20242..22317)
                     /locus_tag="IXG44_000223"
     CDS             complement(20242..22317)
                     /locus_tag="IXG44_000223"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HAQ1656968.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLAGVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLEDVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIDAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAQLEKQSEVDPELFENDAEKELHKAVEA
                     MKASFADQTIHENYDQLVALRPLIENYFDQTMVMADEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(22319..23236)
                     /gene="glyQ"
                     /locus_tag="IXG44_000224"
     CDS             complement(22319..23236)
                     /gene="glyQ"
                     /locus_tag="IXG44_000224"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HAQ1656969.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPETVKEAE"
     gene            complement(23622..24440)
                     /gene="recO"
                     /locus_tag="IXG44_000225"
     CDS             complement(23622..24440)
                     /gene="recO"
                     /locus_tag="IXG44_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HAQ1656970.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKEIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRHIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICERHWSMDDHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRELIDQLYDEYVGIHLKSKKFINQMKSWAHVLKTSEKDMSEKEKETPEKEE"
     gene            complement(24583..25482)
                     /locus_tag="IXG44_000226"
     CDS             complement(24583..25482)
                     /locus_tag="IXG44_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HAQ1656971.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(25497..25898)
                     /locus_tag="IXG44_000227"
     CDS             complement(25497..25898)
                     /locus_tag="IXG44_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HAQ1656972.1"
                     /translation="MPMDLKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFG
                     ILALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKD
                     MAAGAVLVTACFTVIVGLILFVPKIYHLLFN"
     gene            complement(25876..26352)
                     /gene="ybeY"
                     /locus_tag="IXG44_000228"
     CDS             complement(25876..26352)
                     /gene="ybeY"
                     /locus_tag="IXG44_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HAQ1656973.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLELPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISTER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(26381..28573)
                     /locus_tag="IXG44_000229"
     CDS             complement(26381..28573)
                     /locus_tag="IXG44_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HAQ1656974.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIVCGVTFSSVKQKAIDF
                     REGQVAEESIRANKTIENTEETEQRRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDTINKSYNGKVDKAKEDENIPKPTTEERIATLKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEDQIQYLNVTTEQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSVV
                     LMKFFQLFQTEQAAYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTVILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIIYQGMSFGD
                     ATTWLTLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICRQHHGTTLMRYFYVKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKSEVEKIKEEQEE"
     gene            complement(28597..29568)
                     /locus_tag="IXG44_000230"
     CDS             complement(28597..29568)
                     /locus_tag="IXG44_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HAQ1656975.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            30273..31652
                     /gene="brnQ"
                     /locus_tag="IXG44_000231"
     CDS             30273..31652
                     /gene="brnQ"
                     /locus_tag="IXG44_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAQ1656976.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGANWV
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYNSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFHPMGNLNQSAADTYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKTPFVYQVTMLFAAVPAVLDM
                     LANSPALVSQQRVVASMLEFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            complement(31808..32254)
                     /locus_tag="IXG44_000232"
     CDS             complement(31808..32254)
                     /locus_tag="IXG44_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HAQ1656977.1"
                     /translation="MSLLSRLNDDMKTAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(32281..32457)
                     /locus_tag="IXG44_000233"
     CDS             complement(32281..32457)
                     /locus_tag="IXG44_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HAQ1656978.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            complement(32619..33047)
                     /locus_tag="IXG44_000234"
     CDS             complement(32619..33047)
                     /locus_tag="IXG44_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAQ1656979.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            33139..33999
                     /locus_tag="IXG44_000235"
     CDS             33139..33999
                     /locus_tag="IXG44_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HAQ1656980.1"
                     /translation="MSSDSTEIITFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLMQALQDALKEKALVLHTLINQELIEMTRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEETD"
     gene            complement(34093..34386)
                     /locus_tag="IXG44_000236"
     CDS             complement(34093..34386)
                     /locus_tag="IXG44_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAQ1656981.1"
                     /translation="MPDTQKTKVEKVSQLFKMLSDPTRLKILLYLKDGEQNVTAITQA
                     VEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHVLDILNQTLKHVEHQ"
     gene            complement(34459..36186)
                     /locus_tag="IXG44_000237"
     CDS             complement(34459..36186)
                     /locus_tag="IXG44_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HAQ1656982.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDDEAEHSGLITIIGEEVDAAYLEEIKAV
                     TIDHELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAMRLGEKEGADLLIATDPDADRLGAAVRLPNGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYYAEKTISVTMSGQEGSAKISALMEKFREEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGNSSEDAAEKVAAYE
                     AIIKKMTEE"
     gene            complement(36399..37325)
                     /gene="trxB"
                     /locus_tag="IXG44_000238"
     CDS             complement(36399..37325)
                     /gene="trxB"
                     /locus_tag="IXG44_000238"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HAQ1656983.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYEGVSRFGTENAYGIVQDIKDHGSHKEVICEDKTYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNIPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            complement(37470..38903)
                     /gene="ascB"
                     /locus_tag="IXG44_000239"
     CDS             complement(37470..38903)
                     /gene="ascB"
                     /locus_tag="IXG44_000239"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAQ1656984.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGEDRFPV
                     ITGKKKMFEFDENHFYPAKIAIDMYHHYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNESGLKFYEDVFKECQKYGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIKMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLEASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNEGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            complement(38903..40306)
                     /locus_tag="IXG44_000240"
     CDS             complement(38903..40306)
                     /locus_tag="IXG44_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAQ1656985.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPTMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKAEGIEVQ
                     DELARELSSNTHVMEEV"
     gene            40536..41438
                     /locus_tag="IXG44_000241"
     CDS             40536..41438
                     /locus_tag="IXG44_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ1656986.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSSMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVD"
     gene            complement(41479..43365)
                     /locus_tag="IXG44_000242"
     CDS             complement(41479..43365)
                     /locus_tag="IXG44_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1656987.1"
                     /translation="MGKYQELARKIVENVGGKENINGLTHCITRLRFKLKNEEKANDE
                     ILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAVSTEKMRPFDMLID
                     GISGCFQPFLAILAADGMIRGLTAFLVFLGAFDRGSGTFVMFDNIGDSVFQFMPVIIG
                     LTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSGTIFEAPIYQTVFG
                     IPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFFTVLITMPLGFLLV
                     GPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWAIVPMGLMQFSVNG
                     WQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVGITEPAIYGFTLPR
                     KKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGDLTDVINVLIGIAI
                     AMVVSFALTFFFWKEEADETDDRQKRSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGR
                     GILIYPEKGEVRAPFDGTVMSLFPTKHAIGMVSEAGLELLIHVGLDTVQLEGKYFESL
                     VQQGERVKQGDLLLRFDKKKIEEEGYSLETPVIVINYMDYLDILENQGETVGPDDALI
                     TALT"
     gene            complement(43462..44314)
                     /locus_tag="IXG44_000243"
                     /pseudo
     CDS             complement(43462..44314)
                     /locus_tag="IXG44_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
     gene            complement(44649..46433)
                     /locus_tag="IXG44_000244"
     CDS             complement(44649..46433)
                     /locus_tag="IXG44_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1656988.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGVYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYVRVRLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFVAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQEFFNPMSQMMDFLSTFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQAITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGV"
     gene            complement(46433..48184)
                     /locus_tag="IXG44_000245"
     CDS             complement(46433..48184)
                     /locus_tag="IXG44_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1656989.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGIFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIISWWILLLILAAFLQYLFRYIWRTNIWGSAARLERELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     RMWEKQQLEAKIEGGEA"
     gene            complement(48326..48550)
                     /locus_tag="IXG44_000246"
     CDS             complement(48326..48550)
                     /locus_tag="IXG44_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HAQ1656990.1"
                     /translation="MVSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            48801..50387
                     /locus_tag="IXG44_000247"
     CDS             48801..50387
                     /locus_tag="IXG44_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HAQ1656991.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            complement(50453..52228)
                     /locus_tag="IXG44_000248"
     CDS             complement(50453..52228)
                     /locus_tag="IXG44_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HAQ1656992.1"
                     /translation="MLNILNPLIDQLKKKHTYFILGIAVLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIHYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(52439..53341)
                     /locus_tag="IXG44_000249"
     CDS             complement(52439..53341)
                     /locus_tag="IXG44_000249"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HAQ1656993.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAFMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGAEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKVIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     ISMFKAQAFDPEVFPELLAEEVTL"
     gene            53620..55965
                     /locus_tag="IXG44_000250"
     CDS             53620..55965
                     /locus_tag="IXG44_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAQ1656994.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLLFAA
                     LGIGIGAGYFAYLVEDTQLPTKKELQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIVLSAQVEKHFSKNEIITTYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSEAYYQKAQQTIQNKGYTIHSTIDKDIYAALQTGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLVGSES
                     RVSDYATTWQEGKNAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINEKITTPFKDVISSLNGNLGNA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSQARIRSANYLANLINQAY
                     QANPEIFGTDEKFDLSSDVMKKKVSGFTGQTAGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANPETKESDDKDD"
BASE COUNT        16610 a        11680 c         9086 g        18720 t
ORIGIN      
        1 atttaaaatt aacattgatt ctcctatgaa aatatctttt acctttctcc tgtgaaaagg
       61 aaatttcctt ttttatagtg ttattgatat aatcagaata ttcatcaaac tctgtatctg
      121 gagttaaaaa tctacagtaa catctataag ctttgctagt aatcttcttg ggttctctct
      181 ccattgtcaa gcaacatcaa aacacctact tactattcga ttcttttctg ccaaaaacga
      241 gtcagcatca ctcacaactt atgtgttgct gactccgact agtcctttgt tcaatatcaa
      301 cttattaaac agatataata tttttcttta tttcgtatac tccaagactt ccatctttct
      361 ttcatcttat ctctgaaaaa aacacagcaa agtgccctta ataaatatac attagaattt
      421 gcaaattttt tcatcttaag atatatccta ttcatattct ttacaatcct aatgggtgat
      481 tacaaatcta tttagttcaa gttctacatt ataaacacct ttcttgatag tatcatctgc
      541 tgcacaagcg atgtcgatcc acttattagg ggatttacgt gctcaaaagt ggctattcct
      601 tttgggaagt ccatccataa taatggtcca tggatttttt tagggtcttg tcgttcaaat
      661 ttctcaaaaa ctttattata cttccatagt tttataatta tttggtttca ctttggctcg
      721 ttcaaacacc aacttgtatt tcttttcaac gttcgaactg gagccccccc tataatctca
      781 ttattatttt catgactttt tactaccgtg gaattacaag caacaacaat attatctgct
      841 aaatgaacac ctttctatat cacgcttttt gctccaatcc aacaatgttt acctataact
      901 attttattat ttttattcaa aatcctatga gtattcaatt caaaaatcgt atgtgaatcg
      961 gtatccataa ataatgtatc ccacgatact gtacagccat ctttgaattg aatttcctta
     1021 aagcaaatta tactcatttt tcctgtcgaa ttaaactttg acccaatatc taaaactgca
     1081 tttttcctta tgtcaatatg agccccttgt gcgataaacg ctggcccttg tattcttagt
     1141 attccttcaa tacttataat cgtttcttca tttcccgcag tatttaatac tggaaagccc
     1201 aaataaacta tttttccaga ttgtaatact atctcaattt tccctctcga tttgcgtgta
     1261 atccgtactt tacgatgcac caaaataggt aatctccacg catctgaaat atgaagaaat
     1321 ttataattga agtatatcgt tttaggtaaa tttaatatat tctttattat caatccgagt
     1381 ctcatctttc accatctctt tttgtaatca aaattagttt ataaaggatt acacccaata
     1441 taccgcccaa cgtattatat acaaaatccg aaatttgaaa cgttcctagc ctcaaaaaaa
     1501 gttgtaaaaa ttcaattatt gctgaaaaat aaaatgcaat ttttcctcca tatactattg
     1561 aggaccatat tttgggtttc tttatatttt catatttaat agacattacc atataaacat
     1621 atggaataaa aagaataata ttttctaaac acgctgtaga taactctttt gttccagttg
     1681 ttgtattata cttataaatc caccagtctc ccataacatc agataaagga tttaaccaaa
     1741 ttgttcgatt gattaatgtt gtaaataaaa tcatcgcagt atagaatatc aaagcacaaa
     1801 cttttctata ttttctatcc ttctttaaat gaagataaag ttccattagt atacgtttat
     1861 agccctcacc tgcagcaatg ccatctgttg catacataaa atagaccagt cctaaaattg
     1921 tcaataaaac agcaaaccca aagggttcat ataatttata taatatatct gtaataattt
     1981 ctgcaatcac tattatcata attatttctc caatatcaag tatacactta tcgcttccaa
     2041 ttacacatga tcatattaga gtgtattatc acattctcta atattcaatt tatttttcct
     2101 aaaatattcg aaataatcgt cttgataacg agtaagtatt tttatacgat tgataacata
     2161 gtaacaatat ttttaattat agtaaaaaat catctgcctt tttcatccac ctaatcttat
     2221 ttttcatttt attcaacatc cttaaataaa cttccgttcc taagctctat aatatcttct
     2281 taaaaaataa tattatggtg tcgttttcaa actcgtaatc ttttacattg gaaaaataca
     2341 tacttttctc aatgtagcaa ctcctatctc acagtattca aaaattcttc aatattatga
     2401 aatacttgtg catgttcttt aaataccttg tcagtctgtt tataatcccc tactaaattt
     2461 tgcattgctc ccaactcttc gacaaattgt catctccatc tgtaaacagc cctaaaaact
     2521 catataatgc tttttcatca atatttttcc catattattc tcggtatagt ttcacaagtt
     2581 ttacggattc ttcatattgt ttatcttttt ataatattca tctagggaca taattacttt
     2641 tgctagattt cctgcaacaa cacaatcgtc tgatacgtcc ttgtttacta aactatttgc
     2701 acctataact acattatttc ctatatgtac accctttagt attctagact tcattcctat
     2761 aaaaacatta ttacctattt tgatcctctc cgatgatccc aaagcttctc catactactt
     2821 ttaaaaccga tcaatcataa acatgagtca aaattgtaac tccttcatat catctccaat
     2881 ttcaacaaga gtcttcgttg gaacaaaaac tatacatcgt tcgcctattc ggcagcctaa
     2941 aaattgtagt tactttatat atcattctga tgtctcttct catcttttta ccaataccga
     3001 cgtaagaact ttcaaaatat acttaaactt cttattacgg atattaaaca gaagaataac
     3061 taaaatcgat atgacaaccg atataactaa atttatcact aaacttatca ctttgaaatt
     3121 tccgatatta acatacgaac aagcaaaata acttaaataa gaagcaacaa aagctattac
     3181 acacaaactt atgagcttcc aaacatattt acctgccgat tcaaatttaa atatattttg
     3241 gaaaacatta tataataacc agggtaaatc aaccaacaat actgaaacaa tggttgccgc
     3301 caaaactcca tataatccga accaattaac gaggatgata tttaatacaa tgtttatcat
     3361 tgctgtaatt aatggtctaa acctgtctgc gtaccatatt ccagcagcat ctttgtataa
     3421 cccaattaac tgatcaattt catataagaa aaaatgaact atcataaata taacaaatga
     3481 aatactcata agattatcac ttcccaccca gataatcatg aacggctgaa ataatgtaat
     3541 aaagcatgca caactaattg ccagaaacca acctataatt gttgtaatat cacaaaaatc
     3601 tatataattt ttttcttgac tttctacaat aagactgtta cctatacctg ctatacatga
     3661 ttcataaatt attgcaaata tactcattat ggcagaaata ataaagtaat aattctgata
     3721 aatcgcaagt actgaaagac ctaagaaaga tgaaataata attgaatctg aagatcgtaa
     3781 tattgtaccg cctaccttgt ttgtaactaa tccttttact ttttttataa tatcgtcaat
     3841 agtttctttc tctaactcac caacaggagt ataatcagga tacatctttt gagaaacaac
     3901 agcacacatt aagttaagaa ctatttgtcc caaaatagaa gccaataaat acaaatagta
     3961 attttttaag aaaatcagaa catatgcctg cattacaaat tggaatgtaa aagtaattag
     4021 catagacacg ttaattacat catttctttg atgtgcatat attagactct ttctatatga
     4081 aaataaccaa tacgacaaaa cagtatttat caaatacatt aaatacaaaa ctgtcatatt
     4141 taaatttcca ggaatcgaac ctttaactaa aaatctcaaa aaaggaatgc ataacaatcc
     4201 taatgtaaac actatccatc caataattga aaagtacttt ttatatgcgt tcagtaaagc
     4261 acaaatctct tttctattgt cttctgctat aggtttatac atacttacag ttagtgcagc
     4321 acctattccc aattccgcta aatttaacat ttgaaatata gatgtgaaca ggttgttcaa
     4381 tccagcatac tccattccca tctgataaat cataagtgta cgcataaaaa agggaatgat
     4441 aatctgatat aacttaaaaa ttgttccagt aattatattc cttttagcat tttgtttacg
     4501 attaattatc atatcatatg gctccaaact ttctattttt tattgttacc taatactata
     4561 taatttctaa aatcttgaaa tgtaataggt ttcttctagc tctttgatta attacgctaa
     4621 ttttcatgtt tggacaacca caaaaaacta tacattttca atcaaagcaa cccattgttt
     4681 tgtaatttct ttcaaattaa atttttgtac attattatat gcgttcagtg acattctcat
     4741 tgctaaatca gtattcttag taaacattat catgctctta gctaattggt ctatattctt
     4801 atcttcaacc aacatcccat taaaattatg atgtaccaaa tctctcggac cagttttaca
     4861 atcataagaa ataactggta atccataaga ctgtgcttct aatataacca taggaaatcc
     4921 ttcataaaca gaactaagca cataacaaga tgcagattta taatattcct ccacatggtc
     4981 tgaatgccct gctatagtta ctctttgcag atttaaatct tttatctgct ggcgaagatc
     5041 tttttctaat tcaccttttc ctacaatgat aagatcccaa tcatctactt ttttttctac
     5101 aattttccaa gcacttatca tgtcagcaaa atttttctct tccactaatc gccctactgc
     5161 aagaaatctc tttttatcat aatgatattc gtactcgata ttagatatac acggatttgg
     5221 catagttata acaaaatctt ctgataaact atattcatca atatgttttt gacgatcttc
     5281 ttctgtgaga accacaagcc tctttgcata tttttttatc aattttttag ccttgaatct
     5341 cttcttttct ttcagcattt ccattgtcca gtaattaaaa tgttcccaag aaatcaattt
     5401 gcactttgtt cctttaattg cccttgtagt atacaaagac aacaaagtat caatatcaat
     5461 tactatatca aattcatttt tgcatatata cttgtgcaat ttccaaaccg gatataaata
     5521 agttctatat aattttccct ccgttttagg atttaacata taatcaacct taatcgtctt
     5581 atctaaccca aaataaggct ctccactatt ccaaaagctt aaaatagtga catcataacc
     5641 taaatcagac aagctattgg caataatact ccctactctt tcagttcccc cagaacgaga
     5701 aattacacca ctaatgaaac aaattttctt caaagtactt ttctccttta taagtgtaaa
     5761 aattttttca gtgcaagtaa cctgttaaat tttttttgtc caattgttga ttttaatcta
     5821 taaacaaatt tttgtttagg tgttttccaa gatgcattat attgatgaat gcttattgta
     5881 ttactagttt tataaatttt tccagtatca taatccttgg cagcaaaata atccgtagga
     5941 tagatatgca ggccatttat tatttgttcg ctgttatcgc gaaccaatcc aatagattca
     6001 agataatctg taatataata cggactcggt ttcaagttca atgtaccatc attttgataa
     6061 aaacttaatg aatcgtaaat atcccttaaa cctttaataa cagaatgtcc ttcttctgca
     6121 ccaaaaccaa taccgccatt aacccagatt tccccttcaa atcccatata ggcatgattt
     6181 tcacgaagta tatctaacga cttaattagc tcaacatctg tatctaaata aaatccacca
     6241 tgattataaa taatatcaaa tcttgcataa tctggaacaa aaccccattt acgttcttta
     6301 tatgcatcgg ccatataacg gttcttattt aaatcataat ttgattcatt ccattcaata
     6361 atctcataat cagggcaata ctttttccag ctttctatgc actttctatc ttttttcgga
     6421 agtggatttc ccccgaacca acagtaatgt atcttcttag gaatcataat atctctcctt
     6481 ttgtattttt ttcactataa taatgctctt tccttttcac aacttcaggc atggctaaaa
     6541 tcatataaat cagtggcccc gttgtttcac ccctaagtgt tgtgttatac acaaaaaaga
     6601 ataccgacat aattgcaata actttaacaa gacttgcatc tttatccttc ataaaagatc
     6661 tttttgcatt gaaaaatgtc tttattataa aaaataggaa tatcgaaact cctaatattc
     6721 caacttctat aaataaccaa atatgatgaa aatactgata atgaagatta ccaaacgatt
     6781 tataaaactc actgacaaaa aaattggtaa actctgcatt gccaatgcct actcccatta
     6841 ggactaatct aaaattatta ttaaatattt catctactac tctagagata ccagttattc
     6901 gatttaaatc accaacacca tcatatccag aatctcttga cgcatattct aaaagacctt
     6961 gaattgtgaa attatcagca tagctttgtc cattatcgtt caatgtaata aatattgtta
     7021 taaaaaagga taatactatc gacacaagta ttacgcatga taatatcttg atcactactt
     7081 taaaatcaac ttttaaagtg aatactattg caacaaatat tataaataat tcaggaataa
     7141 aaactttcaa ttcagctaat gcagacataa aaagtgctgc acaaacacat aaaataaaat
     7201 ggatgaaatt tttttctccc ttgaaatatc cgattgtaac aacagtaact gtcacacaca
     7261 aaattaaatt caatggcata ttacaacctg aataattcca aaaaactcca cctatcatat
     7321 ctcctacaat tgttccagtt ggataggtga ccatgaatct ttcaattaaa caaagtggca
     7381 gtgaaatcca gaataatttt tcaattatag cgaatacttt atagtaatct ttaattgtta
     7441 aatatgttgc acaatttata tatactatga acatacgagt gttatttcgt atcccccaga
     7501 taatcaatcc tatattttct aaattaataa ctgagctaca aatagaaact agtaaaaaca
     7561 aaactatcag catctcattt ttgctagcat atattccttt tctgcgtcta aagctaagtg
     7621 catttaataa cacaaagata ttaattatat ctcccaaata taaaattgct cttggggcgt
     7681 taaaagtata tacaagaact atggagattg caaaaaatgc caacgtgaac aatgtccaat
     7741 ctgctcctga ccatcttttt attgaaatac gcatcgttat tcaccttctt tcgctaaaaa
     7801 ttattctaaa aatacttttg ttaaattaac ataccctatt atttttgccg gtacacctgc
     7861 aagaattgca ccattctcta gacaatcttt gataacaata gctcctgccg caacaactac
     7921 attgttagct atttttacat taccaataat ctttgcaccc acgcatacac gtacattatt
     7981 tccaattaca ggacacttag gatcgattcc atcatttcca atacagttat tcccatataa
     8041 gtaacaattt tttcctatta tggcgttacc attaataaca attccttgtg tatggtaaaa
     8101 tcttacacct tcatcaaata cattttcacc acactctata ccacattttc ttcccaaact
     8161 attcattctt ctgcaagaca caatatacat aatcgcataa atgatatttt tctttctctt
     8221 tgaataataa taagatgtaa ttctcattcg acgagtatac cgccaagcgt tgtagtccgg
     8281 atgtgatttc attattttta gtaattttgt gtgttgtcct ttagattcta tatatagatt
     8341 cttttccata cgaagaaccg cttttaaata ttctttactg ctaatttcca ttcatatctc
     8401 ctattaattc aatttatact taacaatttt attcggattt cttgcaacaa gagcatcatt
     8461 caatgctact gaatctacaa cgatggatat gttatcttca tcgttgattc caccggatac
     8521 aatattactt ataaaaatcg taacattttt tccaaaagaa gggatgtccg tgatgtataa
     8581 tgcccatttt ccgatatttc cagttattta aacctaacga atttttacaa tagtacaagt
     8641 atcacaatca ttaatatata actggaatat aaatctccaa aaattcacta tagtacagtt
     8701 ttttatgatt aaaatctcct atttctaccc attccgctta aatatgctaa tgttttaacc
     8761 ccaataatgg attggtttag taaaaaatag ttaaatattc gttgtttaat tggattttgt
     8821 ttatatgggt atgagtgaat acaggcaatc aactcatcgt tcataagtat cttagacaaa
     8881 taggagttta aatcaaattc tttattaaaa ctagcatatc taactgcctt ttgaatacaa
     8941 gtacgacagc gtcccaacaa aagtctcctt gttttgttta caaatatctc attttctaat
     9001 atatagctta aatcgccttc aattttttta actaattcaa tattacaatc gaactcatta
     9061 tctctaaaat tatgtgtcaa tgaattatta ttattacatc tataataata gatacaatct
     9121 ttaataaaac ttgttatttt tgaatgttgc aaatatgaaa tatggaatat gatatcttca
     9181 gatatataat ccctttctga cggaaacttg atattgtgct gttgaattat ctcagatgaa
     9241 tacagcgcat gccaaactga cattggcaat ataaaatcga ctttataact cggctcagtg
     9301 ccaatcattc caagcaacac tttttcaatt atagcgttat ttgtaaatat cgtactatca
     9361 tatcttgctg gctttgaaat aatattatta tcatcataat attcataata tccagcataa
     9421 catgtgtctg cattactcct ttttatacca tttaacattg cttcaatata attagctgca
     9481 acaaaatcat cagaatcaat aaaagcaaca tattttcctt ttacatgttc aagtcctgta
     9541 tttcttgcca atcccaagcc agcattattt ttatgaataa tacgaatcct tgaatccttt
     9601 aatgcatagt cttcacatat tttattagat gaatccttag atccatcatc cacaattata
     9661 atatctaggt tcttatatgt ttgattacac actgattcca aacaacttcc tacatacttt
     9721 tcaacattat atacaggtat gattacacta attagatcac tcgaattcat ccttctatcc
     9781 tttcactcac gttttctcaa ttagtcaatg acggcatcaa cgattttctt aaattctctg
     9841 aaaaaaatag actctgatct tgatgatgtg aatgacatac tatcttcttc tagaaaaatt
     9901 atttccttta aattatttat taaatcaata atatgatcct cgtaagtatg ctttcccttg
     9961 gaataatgaa aatatacttt tgtattacac aatattgcaa ttttatttct taatatgatt
    10021 atttcactca ttcattactg ccacatatac acaacttata accaccccct gcaaagttta
    10081 gggtaagtta tcagataata tcttcctctt catcatccag aatacaaaat ctatttattt
    10141 atagaaatat cattaatgtt tttatataat gtaccgtgct tgtactcttg ctctattaca
    10201 agcactaaaa cactactatt catttgaatt cttacacgga taaaaattta catcgtctat
    10261 ttcaatattt tttctctcta ttttattcac ttaataagtt cattacataa tcgaaacatt
    10321 ttttattttc cttgtcatca ctccttgctc cctttttgat acagatttaa aattctatca
    10381 aagaaatttc catcgttaaa tttcgttatt gccaaatcct tcgaacaacg tgaaaatttt
    10441 acgtatttat ctttatcatt gatttttagt attgcagatg tcaacgctga tacgtcacca
    10501 ggttgaaaca aatatccatt ttctccatca cgtactgctg aagaaatatc tcctacatct
    10561 gtagcaatta caggcattcc ataagaaatc gcctctagaa tagatatagg caatccctcg
    10621 ttataggacg gtaaaatcat cacatttgat cttctatagc actcctcctt tttttctccc
    10681 acaacccaac ctgtaaattc aaaatacgat tgcaaattat attcttctat caattctttg
    10741 agcgatattt cttcatcacc tgctccagca atctcaaaaa taaacttttt ttctggatat
    10801 tgttctgcaa tgtttttaac tgcttctacc aaatcgtgta caccttttcg ctttatcaaa
    10861 actcccataa acagaaatcg gcacattttt gaattccact ttatggtata ttctggaact
    10921 acaatagaat tttctacaac aacagtttta caatttggct caatttcttt aattatcttc
    10981 ttccattttt ctccaagtac aatcgtggaa tcagactctc ttaacagcaa gcgaatctga
    11041 ttttgttttt ttacagaaac actatcatac cattttttaa attccgatcc atgaagatgg
    11101 atgattacag gaatatttaa taatctaaac cacttattca gcacatattt cctactaaaa
    11161 ctccctctgt acgacatgtg catatgaata atattaggtt tactcaataa agagatacct
    11221 ataattttag caaatgcata cataaaatat aaagatttaa atattgcatt tccaccctta
    11281 taactcggta taaaagtcat ctcgatattc tttttttccc aattaaaatt gagcatttgt
    11341 gtaattatac ttgtaattcc accttttacc gagctgtcat taccaatcat aagtactctt
    11401 tgcataacgc tttactcctt atatttatat tttttctatt aatacctcta tcgaattaat
    11461 aatcgtttcc gtgttagata catacttgtt aaacatccga actcttgttt ctttaacggc
    11521 atcttctaaa ttttctaatt cataacaagg atagataata ttcatctcat caaattgtct
    11581 aattaactgc agttggtgat catcgacatg ctcaccatac ttagccaatc gaggaacagc
    11641 aatgaccctt ttaccttttt ttacggcccc aattatagca cctgtacctc catgagtaat
    11701 caccatttct gctttttgca tagtttcagt aaactcatcc ctatttagaa atgctttata
    11761 ggaataattt agtggtttat aatcactata accactctgt gcaaatgtac tttcgattat
    11821 tactcctttt tcaattaaat catcaatagc ttttaacaat ctatcaaatt gaaatttttg
    11881 tgaacctaat gttataaata tcattttttt ctcctattat ttaatatatc ccacccaaat
    11941 aaatagcatt aggataaaat tgtagcatag tttcccactg aacataaaat tgatctgcaa
    12001 aatgatacat caattttcct gtctgagtag ggctggttac ttttgcaaac gattcaatat
    12061 aaatcagttt ttttcccatc atttttgtta ttaagcagat agggatcata gctaaaacac
    12121 ctgtacaaat cacgagatcc ggtttttcct ttatatatat aataattgat ttgaataaat
    12181 taataagtat agtacacaaa aaaaactttt cttttctatt tacttgtctc atgtagtacg
    12241 ttttttcgcc ttttgactca gctgaatatt ctgtctgttc tgttataaca aaactctcat
    12301 acttctccat cagtggtttt aacatcatta actgttcata atgcccccca gaggaagccg
    12361 caaaacatat ctttttgttc atattaacct ctctatcagt cacgctgaaa aatatctcta
    12421 gtgtaaactt tttctttaat atcatccaag caggtatcat atcgattggc aataatagaa
    12481 tcacacatcg ccttaaactg ctctaaatcg ttgataacct tacttccgaa aaaagtctct
    12541 ccatctttca gtgcaggctc ataaatcacc acagtcgcac ctttagcttt tacgcgcttc
    12601 ataactccct ggattgaact ctgacgaaaa ttatcactgt tgctcttcat ggtcagccgg
    12661 tatataccga ttaaaactgg attttccctg ttgacatcat actgattata ttcagcgtag
    12721 ttgtaatagc ccgccatctg aagaacacga tctgcgatga aatccttacg ggtacgatta
    12781 gattcaacga tagcctcaat caaatttcca ggaacatctg catagtttgc caacaactgc
    12841 ttggtatctt ttggcaagca ataacctcca tatccaaagg atggattgtt gtagtgagtt
    12901 ccaatacgag gatcgagaca aacaccattg attatctgct gagtattcag ccccttcatt
    12961 tcagcataag tgtctaattc attgaaatag ctgactcgca gtgctaaata ggtatttgca
    13021 aacagcttta ctgcttcggc ttcagtgaat cccataaaaa gagtatccac gttctcctta
    13081 attgcacttt tctgcagaag tgttgcaaaa acatgagcag catttaacaa gcgctcatca
    13141 gtcatatcag taccaacaat aatacgagat ggatacagat tatcatataa agccttgctc
    13201 tcgcgcagaa actctggact aaaaataatg ttcttactcc ccgttgtctc acggactgat
    13261 tcagtgtagc caacaggaat tgtagacttg atgaccatga tggtatcagg attcacgtcc
    13321 atcacaacct caataactga ctcaacagca gaagtatcga aatgctgagt acgactgtca
    13381 tagttagtcg gtgtagcaat aacaacgaaa tctgcggatt tataagcgct ccttgcatcc
    13441 aaagtagcag ttaagtcaag ttctttttct gtaagatact tttcaatata ttcatcctga
    13501 attggagact tccgtttatt aatcagttca atcttttcag ggataatgtc taccgcggtt
    13561 acctggtgat gctgcgccaa tagtgtggcg atactcaaac caacataacc ggtgccggct
    13621 actgcgatgt tgtacttttt cttcacaata tctcctcttt cctctcttaa gtatggagaa
    13681 tgttactcct acggctattc atcatgtcaa aataacacag cttagaatgt tgataaaata
    13741 attttccaga atgtgataaa atctctttca tttataaacc acacatctaa ttttatctat
    13801 tctccacaag acatgtagag tattctttaa taacaagata cgaccttact aaatacttca
    13861 aaaaattatc atatttaatt cataaatata aatcttcatt ttaaatgtat gcccaaaaat
    13921 ttatctgtaa tgcgtcatca aaatctagat tccaaatctt tatcaatttt tatccactca
    13981 ttctaccact ttagtaatat tttctattta taacactttc gtcctacatt gatccccccc
    14041 tctgaagtac agtttttact gtcttaaata aaattttaaa atctaacccc atactccaat
    14101 tttttatata ttccgtgtca agttttgcta cttcttcaaa atcagtaata tcacttctac
    14161 cacttacctg ccacattcca gtaattcctg gcttgatagc aattcttgca cgatgatgat
    14221 aatcatattc ttcatactct gatttaaggc atggtcgaaa tcctataagg ctcatctttc
    14281 ctgttagaac gttgaaaaat tgagggaatt catctaaaga cgtggatcta ataaattgac
    14341 cgattcctgt cttttttgtt ccatctggaa gtattttatt tccgataatt cttggatcaa
    14401 actccatctt aaacataaga ttattgctca tcttattttc tttcattaat tctacttttc
    14461 tttcttctgc atccatatac atacttctaa acttatacat cttgaacggt ttaccattct
    14521 gacctatacg ttcttgtgca aagaaaattg ggcctggtga attaatataa ataagtggag
    14581 caataaagat aaaaataatt cccgttgcaa tacatccaac aatacctgca agaatatcaa
    14641 ttgttcgttt tataaatgcc tgttgagttg tcataacatt gatacttgtt gtaagtactg
    14701 tatattctcc tatacgacca atagattgtt ttcccaaagc actatctgat acttttgcaa
    14761 gattcaaatg tacagtaagg cccatttcag agcatcgttc aataaattct ttcatacacg
    14821 aagtactttt attcaaaata ataaagactt catctaccca tccctgacat aagtaatccg
    14881 ctgcattttt ggaatcagaa acaactgtca ctccctcaac atcttcacca atcatgcttt
    14941 tatctatgat aatgacacca tttattttat acattttata gttatgctct ttcacattac
    15001 gaataactac ttcagcaata ctactagatg taactataat aagtgatttg ttcccctcat
    15061 ttttcatttt atgtttcaag taatgtttcc ataagaggcg agttatatat gtaattacaa
    15121 cgtataaacc tcccatcaag aaaagaacga ctctagaata atcatttcca ccctgaacac
    15181 taatgagata caaacttcct aacagcacca acataatcgc ttgctttaca gaagaagcaa
    15241 attctatata gtaccctctt tttaatacac ctctatatgc ttcaaacatg aaaataacta
    15301 aaagatctgc cagttcaatg aagactgcca tgttttgata cattggaatc acataaggat
    15361 tccaatctac ctggcgtaag acatacgata gataaaaagc aagctggata cacaataaat
    15421 ctaaaataat aaaatcaata tgtttaaccc atccccttga acttttcttg tacattcctt
    15481 tttactcccc tctttttaat aaaataaatt gtattacttg ttttctctcc taaatatctt
    15541 tctgaaggat tattgactcg acaaattggc ttgtggatat tcttatatat agaacatccg
    15601 taagccaact taaaactcct taacaaaatt tttttactca tattgtagat caaaacaagt
    15661 atccaagaaa tcatatttta tttctttttt ctgcttactc aacaacagct aaaagaaaaa
    15721 tcatcataaa caaattcaac taagccaacg atccccttgt gaaatcaagt tttttcttga
    15781 tttacgaata aagagctaaa ataaaccaaa cttcttcttt tttacctctt catagactgg
    15841 ataactgatt tcatccccgt tgatcagatc ctttgcgact tgtttcatct gtactacttt
    15901 ttccttacca aagtcctttg caatctgctc atagcactct tttaagtaaa aggtgcgctt
    15961 agagacccca tgggcatcag aagcagccat ctgcacgaga ttatgtttga ccatcgtttt
    16021 ggctgttttc tggatgtctt tgccaaatac cccaacataa cttggcgcag tgagttgggc
    16081 caaacatccc atatctagga acggaagcaa atgatttggg tcttcgcgaa aatgcacgtt
    16141 gcgttctgga tggacgatta ctggtgtctt tcctagttct aacaactcaa aaaaaagccg
    16201 ttctgcatat aacggcactt ccagtgtcgg aaattcgatc agaagatatg tatcatctaa
    16261 atctgtaaat aatatttcat ctcggtggat gtcttcgatg agcgttccgg taatacgtac
    16321 ttcttgtcct tccaataacg tcaaaggcaa ctgtcttttc tctaattcgg cttgtaacga
    16381 agcgactaga gaaatgactt gtgatttttc attgctgtat tttccattat tgtggtgtgg
    16441 tgtgcataaa atatgcgtga tgccttgctg aatcgctttt tcagccattg caatacttgc
    16501 ttctaagttt tctgccccgt catcgattcc tggtaggata tggcaatgta gatcgatcat
    16561 ttctcttgtc tcctctcttc ttattcctta atttccgtag taataaccag catctttgct
    16621 atgttctgct ccgttgtaca caacacctaa gactcgtgct tgtaccatct ccagcagttc
    16681 ttttgcttta gtcaaggatt ctttacgtga catattttca cgtacgacca ataaagtgcc
    16741 atccgttttt aaagacatga tctgtgcatc tgttacggct actactggtg gcatatcgaa
    16801 tatcaccacg tcaaataact gtcttgcttc ggataggatc tgatccattc ttggcgagct
    16861 taacaattct gatgggttgg gtggcttagg tccactaggc aaaatcgaca agttctcgac
    16921 tggcgttcgt tggatcacat ctgctacact tcctgatgaa ctaagtgctg tacttaatcc
    16981 actggcattg ttcagctgga atgtcttata gacgacgggt ttacgtaaat ctgcatcgac
    17041 tagtagaact cgttgacctg atttagcaaa aacgactgca atgtttgctg cagtggtgga
    17101 tttcccttct ccaggacctg atgaagtcac gacaatcgtt ttgatttgct gaccagctgc
    17161 ggaggcaaac tggatattcg tccggatcgt acgatattgt tcggcgattg gtgaagatgg
    17221 gtttgttaat gtgatcaaac tgactgcatg agtttgtgct gctttttgtt tctgtgttcg
    17281 tgccattttt cttcctccta gactcttgag cggcttctgc gtgatggggc tgttttatcg
    17341 tttgtagctt ttggtcgtgc aggagctgtt ccttttttac ggataccagc gttcaattct
    17401 ttcgctgaca tttctggaac tgttcctaag attgggaatc ctaatgtatc tgtcacatat
    17461 ttttcatctt tcactgtccg atcgagtaat tccaataaga atgccagacc tacacctacc
    17521 atcattccta acattaagcc agtcattaaa ttcaatttat tatttggcga aacaggtgtc
    17581 gtattggcaa ctgcacctga aatgatcgag attttatcaa cattcatcac atcttttgcg
    17641 ttctcttgga aaacttgtga agttgtgtta gcgatctgtt gagcagccac tgcattcgta
    17701 cttattgcct gaatcgagaa catttgtgaa ttttctgcct gattaactga gatggcatct
    17761 ttgatttgtt cagcagtcat tttcacattg tcttctgttt ctaaacgatc tttgacttca
    17821 ttgagcacta aatcactgac gatcatatct ttatacgtat tgatcatttg caggttcgta
    17881 ttgacatcat tgacatttgc agtgtctgat tgtggcagtg tcacgatcaa ttgtgcttgt
    17941 gaactgtatt ttggtgtcaa gatgaaaaat gttacgattc ctgagatggc caagccagca
    18001 aacattgaga tgatgattat taccaagtgt ttgcgtaatg tttcaaaaat ttctttcaag
    18061 ctgattgtct cttccatttt ttctccactt tcctatttat tctctagatt catttgatct
    18121 tttaattctt ttttcacacg atcaagttct tgatcactga ctttttgata ggagatatta
    18181 tcttgcatga agccttctcc ttgcagctga tcttgcttaa tcgtaccaaa tgcctcccga
    18241 taatctaaag caattttttt catttcatca aagttcatgt ctgttttcat attcgactct
    18301 agagcattca aaacggattg atagttgctg acaccgttta gactcaatac ttttttcgct
    18361 acgccttcta cgatctttct ttggcgccct tgtctaccgt aatcaccatt tgggtcttca
    18421 taacgcatac gagaataaga taatgcctga tccccgttca atgtaatttt accgattgga
    18481 aagtcatagc catcttgtga gaaagtgagg tcattattga cttcgatacc accaactgca
    18541 tcaacgagtt cctttagtcc tttcatattg atagagacat agtgatcgat tgggatgtct
    18601 acatattttt gtacggtatc catcgccatg gcagctccgc cgaatgcgta agcgtggttg
    18661 atcttgtctt ttgtgtcacg acctacgatg tcgacatacg tatctcgagg aatgctgacg
    18721 atcgttgtct gtttgtcttc aggactgact gtggcaacca tcatcgtatc tgatcttcct
    18781 tgatcgaccc gacctaaatc tccagtatca atccccatca atagaacaga gaaagattct
    18841 tgtttgtcca gatcaactgg tttcgttcgt tctccttggg ttcgttccac tgattcgtaa
    18901 gttttctcaa tagaatgtga gagatccaga tataattttg ctcctactcc tacaattgct
    18961 aatactaata cgataagaac tgctaaaata atcaaaaata tttttttccc tcttgatttt
    19021 cttcttcgtc ttgccatagt ttcctccgtt tgctaacctt gcttgaatca acgttttaac
    19081 tgtcaatagc ttatattgct atagcccatt tgctgagcaa cagtttgtgc ttttgttgcg
    19141 tatgcggctc ttattttttc attccggatc tgctctattt gactgagcgc cgtcaaatat
    19201 tgatcgtaag tagcggtagc tgttacttga ccgtctttca gcatcgtatc gaatagctga
    19261 tctagttgcg tgacttgtgc aatttgggta tcagcgaatc ctagatattg cataacgact
    19321 gttttccaag cactatcctt aaaaaagctc atattttcac gtacatgggc gacatctgca
    19381 cttgccaact tttctttgat gatgacatca tccacagctt gctgccagtt tgatacgttt
    19441 ttagtaaata atttattgac agccgtctgg atcttcagtt ttgattctgc atcctctaac
    19501 tgcgttaaga ctgctttctt ttcttcttgg atcgcagcag cttctttagg taattctgat
    19561 tccttgatgt cataatcatc tgccgaaagt ttgatagaag atagtttatg tgacaaattt
    19621 gaaactgttt cttctgttat ttcaggattt aggaaaattt tttcttttgt actgtacagc
    19681 ttatctactt cttttgataa agaggtcacg gtgtcttttt gattggtgat ggcagattct
    19741 gcgtaattga tccgttgatg atattgccgc tgattattga gatggtatcc aaacaagcaa
    19801 atcacaatag cgcctgtgcc taccgccaca gtagccaata acttgacgaa cggcttttcc
    19861 atagattcac tcctaaatat tgatgataga taagtaaaga tgtgattcat tcacatcaga
    19921 atgagcgctt tttattttaa aagcataatt aaactaatat attcatagaa atatttataa
    19981 acaggttttc cctatataat cataccacat acagtttttt tgactaggga aattcctaat
    20041 ttaaaagtaa ataaaacagt aacaagatta taattaatcg ttttcatttt tatttcctca
    20101 cttttcatga gcgttttgaa aattcgaaca aagaataaaa taacaaaaat tattaaaata
    20161 gaattaataa cagctattta taacaaaaaa aacagagaca cagcacgttt cgtgcatgct
    20221 ctctgttttt caaacaaaag actattttgt gatgatttga tccaagcttg ctaatgacaa
    20281 agccatggca gctatttgac ttagttgtgt tagtcggttc ttacgtactg ctggttcatc
    20341 agccatgacc atcgtttgat caaaataatt ttcgatcagt ggacgaagag cgacaagctg
    20401 gtcgtagttt tcgtggatcg tttggtcagc aaaacttgct ttcattgctt ctactgcttt
    20461 gtgtaattct ttctctgcgt cgttttcgaa cagctcaggg tctacttctg attgtttttc
    20521 aagctgcgct tttttcgcta agttcacgac acgtgtcaaa gcttccattg aaggtttgaa
    20581 gtcttcatct gcaagatgtt gtttgaacaa ttgtgctgct gcaaatactt ttgtcagatc
    20641 tttttgttcc gcattcaaaa cagcatcgat gacatcatga cgaatattct tcgttgtcaa
    20701 taattggcgc agacggcctt tgataaagtc gatcacttgt tgttgccctt ctgataaacc
    20761 aatgccgaaa cgatccgtat ctttactgat aacatcgcta atcgtttctt gcagaacaga
    20821 caaaggaaag gcccattttt tcgattcgat gatccgaacg acaccgtatg cttggcgacg
    20881 taaggcataa ggatcgtttg acccgcttgg aatcatccca actgcaaaga aagaaaagat
    20941 gctatctaat ttatctgcaa tcgctaaaac agaacctact gtagactgag gtagctctcc
    21001 ttcactggat ataggcatgt aatgctcacg aatcgctgtc gctacagcag gtttctcgcc
    21061 tttcagaaga gcatattttt cgcccatgat cccttgtagt tcaggaaatt cccctaccat
    21121 gttggttact agatcaaatt tatagatttc agcagctcgt tgcaaatcca tcaattcttc
    21181 ctcagatagt cctacttgtt cgccaatgat acgggcaatg acttgtaccc gttgcatttt
    21241 ctcatagata ctaccgattt tttcatggaa agtaacagtt tttagttttt caacgtattg
    21301 gtcgattgaa gctttcttgt cttcattata aaagaattcc gcatcttcta aacgagccgt
    21361 caatactttt tgattgcctc gaaccacatc ttccaatttt acccgatcac cattacgagc
    21421 agaaataaaa tgaggaagca gcataccttg atcattccgt acctcgaaat aacgttgatg
    21481 ttctttcata gaagtgacta gtacttcttc aggaacagaa agatattttt cttcgaagtc
    21541 gccagaaaag acagttggat attcgactaa attgttgact tcttctagta aatcttcgtc
    21601 tagatcaata acccagtgat ttttttctgc taattcgttt gcttgtgtaa cgatcatttc
    21661 ttgtcttttt tgcggagacg caatgacgaa ttgttcattc aatttttctt catattcttt
    21721 tgcatgcgca aatgtcacat cttcgcctaa gaaacgatga cctcgagagg ctctgcctgt
    21781 tttgacatcc aagatttcga atgggattac ttcgtcatct aacaatgcca cgatccagtg
    21841 aatagggcgg atatattcaa aatcgtattc tgcccaatgc atcgttacag ggaaagtcaa
    21901 acttttgatc acccctgcta agcctgtcaa tacttctttt gcttccttac catgagtgta
    21961 tttcgttaca tagacatatt ccacaccttt taattctcta aaagtgatag cgtctgtcgt
    22021 cagaccttgt ccgcggacaa atccttccgc tgctttcgtc cagtttccat ctgcgtccaa
    22081 agcaattttt ttggctggtc ctttgacttc ttcttcactg tccgcttgtc gttctggaat
    22141 atttgttact ttgattgcta aacggcgcgg tgtggaaaat gtttcgatcg tatcataagt
    22201 caattggttt tcatctaaaa attttgctgt tttttcttct aattgtttca tgcttggtgt
    22261 gacaacatgg gcaggcattt cttccagccc gatttcaagt aataagtctt ttgccatctt
    22321 attcagcctc cttcacggtt tctggatttg ctgtgttttt attcaacaaa gggaatccta
    22381 atttttcgcg ttctgccaca aagattttgg ctacagcacg agccatgttg cggatgcgag
    22441 aaagataacc ggcacgctct gttacggata ctgcaccgcg ggcgtctaat aagttgaatg
    22501 tatggctaca tttcaagata taatcatacg ccggatgaac aagattttca tcgatacagc
    22561 gtttagcttc tttttcaaaa cgatcaaagt tgcttagtag caagtcttgg tcgcttactt
    22621 caaaagaata tttggaatgt tcatattctg gttgtttgaa gatctcgcca tatttcacgc
    22681 catcagccca ttctaaatca tagacacttt caacttcttg gatataagaa gcaagacgtt
    22741 ctaatccata agtgatctca gaagttactg gtttacaagg cagtccacca acttgctgga
    22801 agtacgtgaa ttgtgtgatt tccattccgt caagccatac ttcccaaccc acaccggcac
    22861 agcccatgga tgggttctcc cagttgtctt caacaaaacg gatgtcgtgt tctaatggat
    22921 cgattcctaa aaggcgcaag ctttctaaat aaagctcttg aatattctca ggtgaaggtt
    22981 tcatcaccac ttggaattga tgatgctgat acaaacggtt tgggttttca ccataacgtc
    23041 catcagctgg tcggcgagat ggctctacgt aagcagcatt ccatggttct ggtccgattg
    23101 cccgtaaaaa ggtatatggg ctcatcgtac ctgctccttt ttctgtgtca taagcttgca
    23161 tcagcatgca gccgtttgat gaccaaaatt tttgtaatgt taaaatcata tcttggacgg
    23221 tcaatttttt actcatctgt tttgcccctt tcaattttaa agcgttgctg tcaaataaga
    23281 atacaggaaa gaaaacgaca aaaaatccct atgtctgatt acagacatag ggacgaataa
    23341 ttcgcggttc caccctactt ccgaataaag attcggcagc ttcatttatc gtactcgcaa
    23401 gtgcctcttc aaaaatcgtt tgggtccggc tcccactctc ccgaactcgc taaaccacgt
    23461 gttttttact gatcttggtc aatgtacgta ttcattttgc agttttcttg tttctataat
    23521 agatcctatc actgaacgat ctatttaatc tatggttaat aatagacgga actccctgtg
    23581 tttgtcaaat ggttttgatt ttttgttgta tgttttattt actattcttc tttttcaggt
    23641 gtctcttttt ctttttcaga catgtctttc tctgacgtct ttaatacgtg tgcccaagac
    23701 ttcatttgat tgatgaactt tttgcttttc aaatggatac cgacatattc atcatacagc
    23761 tggtcgatga gttcacggat tgcttgtttg gtttcttctt tcagatggat tccactaatt
    23821 ttatcataag aaatagctga aaacatccga acgaaataaa cagctcgagg atctgcatga
    23881 taacggtgat cgtccatcga ccaatgtctt tcgcatatca caccaccata tttggaagaa
    23941 tagtcaaatt ttccttttgt ttccccgcat acgacgcagt gtgtccagat tggtgcaatt
    24001 ccgaagcgtt gcagtatttg gacttcaaaa atattcgtga tgatttctgc atcgtctcct
    24061 tggtccatcc gttgcagtgc ttgttgcgta aactggaaca aatgcgggtc gtactgatga
    24121 tcttcgattg ccgcatctac tagattcaat aaatatgtgc catagccgct caaaaagatg
    24181 tcttcttgga tgtgtcgaaa cgcatggaca tctttgacac tgcttaaaaa tgacaaacct
    24241 tcacttcgaa aattcccgat ataaactgct tcagtaaaag ggagcaaagc cggagctaag
    24301 ggctgatttt gccgatggat ctctttgata taaaacatca ttttgccaga agattctgta
    24361 aagattttga ctaatttatc tttttctttg taattacggc tagataaaat caagcctttt
    24421 gattctgcaa gctggctcat attctccctc ctaaaaaata aatctggaaa agcagattct
    24481 gccgatccag atcatttatg ttgcgatctt ccgcagcttt tctcaacacg cggattttta
    24541 ttcagtttat ttctatacct aatttacaag aagctcagaa tatcagtatt cgtcttttcg
    24601 gtagccgaag tcttgcagat agactttttt gtctcgccag tctttttgaa ctttgaccca
    24661 aagttctaga aagactttgt ctcctaaaag attttcgata tcttttcttg ctttcgttcc
    24721 tacttgcttc agcatttttc cgcctttgcc gataatgatc cctttttggc tatctctttc
    24781 gacaataatc gtagcctgga tatggatttt atcattctcg tttcgcttca tcgaatcaac
    24841 aacgactgcg acagaatgag gcacttcgtc tcgtgttaaa aacaagactt tctcacggat
    24901 caattcagaa acgatgaagt attcaggatg atcagtcact tgatcatctg gaaaatattg
    24961 tgggccctct ggcatttcac tgactaatac atccatcaaa cgttcgacgt tgtttccttc
    25021 tgtagcagag attggaatca cttgtgcaaa aggcatctgc gttgaataat cttcgatgat
    25081 tcccaacaac tcgtctggat ggattttgtc gattttattg ataatcagat aaacaggaga
    25141 atgcacgttt tttagacgtt caataataaa atcgtctccc cgaccgcgtt tttgatcagc
    25201 actaatcata aataagaccg cgtccacttc tttcaacgca ctgtaagctg cttctaccat
    25261 aaaatctcct aaacgatgct ttggtttatg gattcctggt gtatcaataa aaatcaactg
    25321 agcttctggt gtagtgtata ctccttggat tttatttcta gttgtctgtg ccttgtcact
    25381 catgatggca attttttgac ccacgattcg attcaataat gtggatttcc ctacatttgg
    25441 gcggccgacg attgcgacaa accctgattt atgttctggc atatatgttc tcctttttaa
    25501 ttaaacaaca aatgatatat tttagggaca aataaaatca acccaacgat gacggtaaaa
    25561 caagcggtca ctaaaactgc tccagctgcc atatctttga tttttttccc aatcggatga
    25621 aaatgaaaat ccgtaaacat gtccacgaca ttttcaaaaa cagtattgat gatctctact
    25681 atccatacga gaaaaacagc taaaagcagc cataaccatt ctatccgatc aagttggaaa
    25741 atcacgccca taaacaaagc gaggattccg aacgcaacat gtttcctcat gttgcgttct
    25801 tcctcaaaaa cagttttgac tccttggacg gcaaattcca cggacatcag gaaatgtttg
    25861 tttttctcta cgtttttatc ttttaagtcc ataggcatct aagatctctt tctgtaaacc
    25921 aaacatttct ttctcatcct ctggtgtcat atggtcatac ccgttgatat gcaagaatcc
    25981 atgaaccgct aaaaagccaa gttcccgatc atacgaatgg ccgtaagctt ctgcttgctc
    26041 tgcagcacgt tcagtagaga tcatgatgtc tcccagtaca cgaggcaatg gattctctcc
    26101 atcttcaaaa atcactggga tttcgtcttc gccctcttct tccaaggcaa aactgatgac
    26161 gtctgtcggt gcatcttttc cacggtaatc acgattgatt acttggattg ctgcattgtc
    26221 catgaaagta acagacattt ctgtttcttc tggcaactct aaatatgatg ctgcaaattg
    26281 cagcaagtcg tcgatttctt tcattttctc ttctgagact tggtttgttt catccatgaa
    26341 ggtaatatcc attatacatc gcttctttct attcttcttg ctattcttct tgttcttctt
    26401 ttatcttctc aacttccgat ttcattttag gatatttgat ccgagaatgg aaggtactgc
    26461 ttaaaccgct gatcaatgat ttttctactt tacggatttc ttttaacgtc aatccactgt
    26521 cgtccaattg tccatcggaa atacgctctt caataagatt atggacaaaa ctttcgattt
    26581 tttcgctcgt tggatggtcc attgcccgta ctgccgcttc acaactgtca gctatattga
    26641 cgattcctgc ttcacggctt tgaggttttg gtccaggata acggaattgc tcttccgtaa
    26701 cttccgggtt gcgttccttt gccttaacgt agaaataacg catcagcgtg gttccatgat
    26761 gctgtctaca aatatcgatc accatttgcg gcatattata atcttccaat attttggcac
    26821 catctgtcac atgtccaaag ataatatgtt tactgtcttc tggcaataag aaattgtgcg
    26881 gattttccgc tcctgaaggc aagttctcaa cgaaaaagct ggcgtgtttg atcttgccga
    26941 tatcatgata atagcaagct actcgagtta gtaaagaacg tccaccgatt tctgctacag
    27001 cattagcact tagattggct accatcatgc tatggtgata cgtacctggt gcttcttcca
    27061 gcaactgttt caataaagga tgattgggat tgctgagttc atttaatacg atcacactat
    27121 catcattgac catcaattca atataggggt gcagacctat cgtcagcaag aatgaaaaca
    27181 gtgcacctgt gaatccgcag actagcgtta accaagtcgt tgcatcccca aagctcattc
    27241 cttggtagat aatcaaaatg acatccatca tcacaggaaa aataatgacc cacatagcag
    27301 cggataaccc ttgttctgag atccgtttgc gtctgacgat cgttgccatg agaccagaaa
    27361 atacatagga catcaaaata actgtcagaa agttcgtgcc aatcgaatca taaaaaatga
    27421 aaagtgcgga tactgcttga aaaataccag ccatgatccc tgctcttcga ttcaagaaaa
    27481 agcttaaaac aagtggagca aaagccgcgg ggtaaaacag cggaatataa gcagcttgtt
    27541 ctgtttggaa gagttggaaa aatttcatta gtacgacgct tactgacatt gccgtcacgt
    27601 agaaaagtac gtattttgtt ctttctcctg cattttggaa ttgaagtgaa ttataaatca
    27661 gtacaccgat ttgaagcaaa acagctaaaa ccatggcaat gagtgggaaa atagaagtcg
    27721 tttgattcgt caatcctagt aattccaatt ttttcatcgc tgaagcatca atttgatttc
    27781 cttcgcgaac aataatttct ccttggaaga tcatcactgg ttgtacagct tctcgtgcag
    27841 attgttttaa ttcttctgtc tttttctcat tcaatacatc attaactacg atgccttgat
    27901 ccactaagta gcgaatcatt tgttgctgct ctgttgtgac attcaaatat tgaatctgat
    27961 cttctgcttc ttgtttaaaa gcttccaatt cgctttcacg aacttgttta ctcatctgtt
    28021 cgtcaatgag cttcaaactt tcatccctaa ctgtgtgaat ttccgtttca gtcatttcaa
    28081 atatcgtcga ataaaaattg ttcggcagtt tttggtaaaa cgcaacgttt tctgcattca
    28141 cgttttcaaa atttttcttc agtgtagcaa tccgctcttc tgtagtaggt ttcgggatat
    28201 tttcatcttc cttcgcctta tctaccttgc cattataaga tttgttgatg gtatcattcg
    28261 tcttatcgat caattcgaat aattgcttga tccgattgtt ctggtcatct gccagatctt
    28321 tttgataagt atattctgga gtcactgctt ctgctgccag ttttcttctt tgttcggttt
    28381 cttccgtgtt ctcaatcgtt ttattcgcac ggatactctc ttctgctact tggccttctc
    28441 tgaaatcaat ggccttttgt ttgacactac taaatgtaac gccgcagaca atgataaaaa
    28501 acaagaacag aaggaaaggc aatgctgcct ttccgagttt ttcttgtagt ttctgcagtg
    28561 ttttattcaa catgtgctta tcctcctaag caaagcttat tcttgatgaa gatccgcctt
    28621 ttcataagct ttgatgattt cggcaactac agggtgccga acaacgtcac ttgcttcaaa
    28681 attaacaaaa tcgatttttt tgatttgctt taatgttcgt tctgcatgaa caagaccact
    28741 tgtcgtccca cgaggcaagt ctatctgact tgtatcccca ttgacaatca tttttgattg
    28801 ataaccgaga cgggtcaaga acattttcat ctgagcgact gtcgtgtttt gtgcttcgtc
    28861 caagataacg aatgcatcat ccaatgtccg cccccgcatg taagctaaag gtgcgatttc
    28921 gatcacgcct cgttccatca atcggttcgt atgatccaat ccaaaaattt ggtataaagc
    28981 atcataaaca ggccgcagat aaggatccac tttttctttc agatccccgg gtagaaagcc
    29041 taggttttcc cctgcctcca cagcgggccg cgtcaaaatg attttttgca cttcgccttt
    29101 ttttagggca gcaattgcta atactacggc aagaaacgtt ttccctgttc cggctggccc
    29161 tacaccaaat acgatgtcat gtttggctac cgattcaacg tatttctttt gaccgctgtt
    29221 ttttacccgt ataggttttc cattacggtc tttgacgatc tcttcctcat acatctcaac
    29281 aaaataatct aatgtacctt tttgccccat tttcaatgcc gtcacaacat ctggcgtact
    29341 tacgtggatt cctcgtttga tcaattcctg taatgtccga atgactgagg cagccaagga
    29401 aatctgggat tgctcaccaa tgatctgaat gatttctcca cgtgtatgga tggttgtttc
    29461 cgttgtttcc tcgatcagtt ttatatgctt gtcgtgtgca cctaaaagca tgctgacatc
    29521 atcagcagct gtcaatctaa tctctaaaga actagactct tccgtcaaaa atagacatcc
    29581 cctttttttc gattcattct ttcctatttt accatactca gaagaaacta tatagcaaat
    29641 aagaaaaggt tgtaaaagag tcgcttaacc atacgtacca agaaacgtta aaaaatagcc
    29701 gtttccattc gctaaaggtc tcgttttgat aaaaagaagc tgagaatgta agttatatga
    29761 aagtggagaa caaaatattt cgtttcattc aacaagtatt ctctaggtat ttttcctgtt
    29821 tcaaaaaaag aaaacaaaat agttaaaata ttcttgcatt tcaaaaaaaa caggtgtatt
    29881 attacgtcaa caaataaaca tacagcaaag cgtcgaggaa aacagtaggc tattcaacga
    29941 tttattccca gtgagtcatg taagtgagaa atgacaataa atcgaataga cgaacatcat
    30001 ttccgagtca atgtcgaaaa cgtagatcat tgtggctgcc tccattatca ggctagcgga
    30061 taactttatc aaaaagcgtc cgtgaatgaa gaagcttttt taagcgaaaa aaggtggtac
    30121 cgttctagaa tctagagccc ttttcccaag tggaaaaggg ctttttttta ttttaaggtg
    30181 gtggttttca tggaatctga tattagtggc attactaatt gctgcaacgt ttatatcgtc
    30241 aatcaaaatg ataattattg gaggaaaaat ttatggaaga aacaaaaact tattcatgga
    30301 aacaactatt gatggtcagt tcacttatct tcgggatgtt cttcgggtca ggtaatctaa
    30361 tctttcctgt ccacttaggt caaatggctg gcgcaaactg ggtcagtgct ggcgtaggat
    30421 ttgctatctc aggtgctctt ttcccattac tagctatctt agctgtagta gtgacaaaaa
    30481 gtgatggtct gtatgactta gctaaacctg tcggaaaatg gtatgctgcc ttgttcttaa
    30541 tccttgttca tttgacgatc ggaccatttt tcggtacacc acggacagct gctactgctt
    30601 ttgaaatggc tgctaaacca tttttgcctg aaaaatataa ttcattaggt atgtttttat
    30661 tctctgctct tttcttttta cttgcttatt tccttactgt ccatccaaca cggttggtca
    30721 aatacgtcgg aaagtatcta aactccttgt ttcttgcatt attagcaatc atttttgtcg
    30781 tttctttatt ccatccaatg ggaaatctaa atcaatctgc tgcagatact tatcaaacaa
    30841 acgctttaac aaatggtgtc ctagaaggtt ataacacggt tgatgcagta gctttgcttg
    30901 ccttaagtgt cacttttgtc catgcagtcc gtgaccttgg tttcaaagat aaaaaagtca
    30961 cagaattaac ggcaaaagct ggtagcctga gcgttgcgct agaagtcatg atctattttg
    31021 gacttgttct tctcggtgcg atgagtttga ataagatgtc tttatcagaa aatggcggaa
    31081 ctgcactttc tcagatcact aatttttatt taggtaactt cggcgctagc ttcttaggag
    31141 tgatggtcac attaggtgtc ttcactaccg ctatgggact cgttgtttct tttgcgcaag
    31201 atttccataa actgttccct aaagtcagtt atatgacatg gctgcggtta acgactttcg
    31261 tctcttttgt cgttgcaaat gctggtttag acaatatcat ccaatggtct ttaccagtat
    31321 tgatgctttt gtatccattg tcgcttgcgt tgatcttgct ttccttaaca gcaaaatttt
    31381 tccaaaagac accatttgtc tatcaagtaa cgatgttgtt tgctgctgtt cctgccgtat
    31441 tggatatgct ggcaaattcg cctgctttag tgagtcaaca aagagttgta gcaagcatgc
    31501 tcgaatttta tcatcatcac gttccttttg ctgcattagg acttggctgg atggtcccaa
    31561 cgttacttgg ttacgctggc agcctactgt tttattacgc ctatcgccta tcagggtata
    31621 aacaagaagc aaatgaacta ccagaagaat aaccaaatca aacacaattt aacataagaa
    31681 atattaaaca ggaaaaaacc agctagatga aagaatccta ttctttcgtc tagctatttt
    31741 ttagttctct tttttacgcg cacaaaaaaa ctgcggacaa tgattgtccg cagtttaaat
    31801 gataaaatta agattgtaat agttctttca caatggcatt tacttggttt ccatctgctt
    31861 ttcctttgac tttaggcatg atcgcaccca tgactttgcc aaattctttc attgatgaag
    31921 caccagtttg tgtgattgct tcttgaacaa gttgtcgaat ttcttcgtcg gaaagttgtt
    31981 ccggcatgta tttttcgact atcacaattt cactttttac tttttcagct aaatcgtcac
    32041 gtcctgcttc ttcaaactca tgcaaagaat cacgtcgctg tttcatttcg cgagacaaca
    32101 ccgttaattc ttcttcttct gtcaagtcgt gaccttcttt aatctgctcg ttttgtatag
    32161 atgacttgat cattcggatg acttgtaaag attccttatc tttcgctttc atcgctgttt
    32221 tcatgtcatc gtttaatcta cttagtagtg acacactacc aactccaatt catcacagaa
    32281 ctagaatttt ttgcgttttc tagctgcttc agattttttc ttgcgtctta cacttggttt
    32341 ttcgtagaat tcacgtttac gagattcttg taaagtaccc gcttttgaca cggaacgttt
    32401 gaagcgacga agagcatcgt caagtgattc gtttttgcga acgactgttt ttgacatgtt
    32461 aattccctcc ctccgagctt aaaaagtaac cgtaattgtt attgaaatat atctcaaaaa
    32521 acaagactgt cctgtaaata ttatatctaa tgctgttttg accgtcaagg aaatcttgct
    32581 tttttttaaa attatcaaat gttattttat ttaaatcctt attgacaatt ttcacatctt
    32641 ccgaaaatct cgaatctatg cccttcgatc gtacatccac cgagctgttc ttcgaaataa
    32701 ttcattggac acatatggat ttctttcgtt gttccgcaca ccgtacagat aaagtgatgg
    32761 tgatggccga tttccccttg acagcaatga aaacgaaact tcatctctcc atttaaatcg
    32821 gtttcttcca gtaaatcgat ttctgcaaaa tcatggagat ttcgataaat cgtatcgtaa
    32881 cttactcctt tgaattcctg attcatatag tcaaaaactt ctttagcgga tacgtagcga
    32941 ttgcgcttca ctaaataagt taacaatgct tctctttttt tagtatattt aaaaccgtta
    33001 tttttcaatg tttctagtga ttgttcaatc aatgtctgtt ttttcatgac ttattcctcc
    33061 aaatcaaaat gattacgaag tacacattct agagtatggt ataataaaca taaaaaaaat
    33121 gcaagtgagg ttttatccat gtcttccgat tctacagaaa tcattacttt ttttgtcatc
    33181 tctgactctg ctggagaaac tgcgacaaaa ttagcgcaag ccactatggc ccaatatcct
    33241 accgttgagt tcaatttatt ccgcagaaca tttgtgacag atgagaaaac attgatgcag
    33301 gcactgcaag atgcactaaa agaaaaagcc cttgtcctac acacattgat caatcaagaa
    33361 ttgatcgaga tgacgcgttc tttttgtgaa gaacatcaat tatttagttt tgatgtgatg
    33421 actccgccga tatcagaaat cgagcgctta actggtatca agcccattcg gcaacctggc
    33481 gcccttcatt tattgaatga gaactatttc aaacggatca aagcaatgga atttgctgta
    33541 aaatacgatg acgggaaaga tcctcgtggg tttctagaag cagatgtcgt tttgctaggc
    33601 gtatccagaa cttccaaaac gcctttgagc ttgtttttag ctaacaaaaa tttaaaggtt
    33661 gccaatctac ctttgatccc acaagcacat attcccaaac agctttggga gatcgatcct
    33721 aaaaaaatcg tgggactgac gaataaccca gatattttaa acaatatccg taaagaacgg
    33781 atgcgctctt acggattaaa tcctgatact gcttattccg atatcgaaaa aatacgagct
    33841 gaactcgatt ttgcgaatga tctttatgaa aaattgggct gcgtagtaat caatgtcgct
    33901 tctttatcta tcgaagaaac agcttcactt attttgaatg ctttagacct agaagatcat
    33961 agttattatg gaactgaaac caccgaagaa acagactaac agtttttctt gtctttttct
    34021 taaaaaaact ggactgtgtc agcgaagaag attcgttgat acagtccagt ttgtctctat
    34081 ttgcacctat cgttattgat gttccacatg tttgagcgtc tggttcaaaa tatccagtac
    34141 gtgagaatca tcaagactat acaaaatcgc ttttccctca cgatgggact tgacgacatg
    34201 gttttctctt aataaacgca gttgatgcga tacagcagat tgttccattt ctactgcttg
    34261 ggtgattgct gtcacatttt gttccccgtc tttcagatac agcagaattt tgagacgagt
    34321 ggggtcactc aacattttga atagttggct gactttctct acttttgttt tttgcgtgtc
    34381 tggcaatcca ttcacctcca acttgaaaaa agactgactt ctaaagttta ccactttttc
    34441 catcagtctc aagttttttt attcttctgt catttttttg ataatcgctt cataagcggc
    34501 tactttttca gctgcgtctt ctgaagagtt tccttttaca ccgatataaa atttgatttt
    34561 cggctcagtt cctgaaggac ggatagcgat ccagccattt tcttccagcg tatacttcaa
    34621 tacgtcagat ggcggtgtcg ttagtttttc tacgtttcct tcacggtctg ttctcgtcaa
    34681 ctctttgaag tcttctgttt ggatgacttt tgctccggca aattctgtcg gtgcttcttc
    34741 acggaatttt tccatcaaag cagagatttt tgcagagcct tcttgtccgc tcattgtcac
    34801 agaaatcgtt ttttctgcgt agtagccata tgtttcaaaa atcgtttgca agccgtcata
    34861 caatgttttg ccttgttttt tatagtaagc tgctacttct gctaataaga gcaatgcttg
    34921 aatcgcgtct ttgtcgcgaa cgaaaggttt aactaaataa ccgtaacttt cttcgaatcc
    34981 gaacataaaa gtatgggaat gatcttcttc aaattgttga attttttcag caatgaattt
    35041 gaatcctgtc aatacattga acatggtagc cccatagtgt tccgcaattg ctgtaggcaa
    35101 ttcgctagaa acgattgatt tcaataccgc tgcattttca ggtaaagtgc ctcgcgattg
    35161 gtgtgcttcc aaaatatatt gagcaaacag ggctccgatt tggtttcctg tcaatacttc
    35221 ataggagcca tttggcaaac gaacggctgc gcctaaacgg tctgcatcag gatccgttgc
    35281 aatcagtaag tcagcgcctt ctttctcgcc aagtcgcatc gcatactcga aagcagagtg
    35341 ttcttctgga ttcggtgatt tgactgtagt gaaatccggg tctgcgacag cttgttctgg
    35401 aactagtacg aattgttcga atccagcttg tttcaacgca cgttcgccca acatttttcc
    35461 tgttccgtga agtggtgtgt acacaagctt caaatcttta cccattgttt ccaccaattc
    35521 atgatcaatc gttactgctt tgatttcttc tagataagcg gcatccactt cttctccgat
    35581 gatcgtgatc aatccgctat gttcagcttc gtcgtcagat aatacttcaa cttttaacgg
    35641 attgctgact tcacggacat atttcgtcaa tgcatctgcg tctgcaggag gcatttgtcc
    35701 cccatcttct ccgtagactt tatatccgtt gtaagctgct ggattgtggg atgcagttac
    35761 catgattccc gcaaacgtct tcaaatgacg gactgcaaaa gaaagctctg gtgttggacg
    35821 taaactttcg aatacataag aagggatatt gtgatgtgct aatgttttgg ctgcttccat
    35881 ggcaaattct ggtgattgat ggcgtgagtc ataagcgata gcgacacctc ggcgttttgt
    35941 ttcttcacct tgtgtatcca tgaaacgtgc aagtccttct gttgcttgtc ttaccgtata
    36001 gatgttcatt cgattgatcc caggtcctaa gattccccgc atgcctgcag taccgaattc
    36061 caacggtgca taaaaagcat cttcaagttt ttcaggatct tcacccagat catttaattg
    36121 tttttttaag ttttcttcta aattttcttc atgtacccat tgttcataca cttgttccca
    36181 agacatgaaa ataacacctc tttctgaaat atggttctta ataagtatag cattcactta
    36241 ttagatatga aagtatgagg accaaaaaac gcttaaatca agaaaagatt cttttatcaa
    36301 gcacggttac aagtattcgc ataaatcgga ttcagtagat ttcacgcaaa aaaaagagta
    36361 aaagaacaat tgaaaccgtt ctttcactct ttttaaactt atgcagttgt ttcttctaaa
    36421 gattccaagt atttatacac ttgttgtcca gcaatcccac catcaccaac agcagtagtg
    36481 atttgtcgca aatctttttc acgaacatct ccgatggcaa aaacgcctgg tatattcgtc
    36541 ttcatttcgt tgtctgttgg aatccagccg gcttcgttcg tcaacccagc ttctctaaat
    36601 ggtgcagtta atggatccaa tcctacatag atgaatactc cgtctgctgc caaatcaaac
    36661 tcattgccag ttttgacatt acgtgctttc acacctgtca ctaccatttc atttccaacg
    36721 atttcgtcaa cgactgtatc ccacataaag gaaatttttt cattcgcaaa tgcacggtct
    36781 tggatgatct tttgtgcacg taattcatct ctacggtgga cgatgactac ttcagaagca
    36841 aattgtgtca aatagatggc ttcttctact gctgagtctc ctcctccgat cactactaga
    36901 cgtttgttac ggaaaaatgc accatcacaa actgcacagt aagaaacgcc acgtccagca
    36961 aattcttcct cgccaggtac gcctaattta cggtggatac agcctgttgc gataatgact
    37021 gtttttcctt cgtaagtttt atcttcacaa ataacttctt tgtggctgcc gtgatccttg
    37081 atatcttgca cgattccata agcattttct gtcccaaatc ttgaaacacc ttcatacatc
    37141 ttataagcta gatcaggacc catgattgaa tcgaaaccag gatagttttc gatttctgcc
    37201 gtattgttca tttggccgcc aggagcgcct tgttcgatca tcaatacgga taagtttgaa
    37261 cgtgaagcat ataaagcagc agtcatccca gcaggccctg cgccaataac gattacatca
    37321 tacatatgta gattctcctt tacttaatca agtaagcata ctttacagcc aatgcgaaaa
    37381 aaagtctatt gttctgcttg cgtttctcga atagctttat tatactcgaa aaatccgctg
    37441 aaaaacaata gacttttata aatcattgat caatcgttcg aaagattttc tccatttgaa
    37501 tcaattactt ttttatacca gttgaatgat ttcttcttcg aacgggctaa tgtgccattt
    37561 ccttcgttgt cacgatcgac ataaatgaat ccgtaccttt tcttcatctc acctgtacca
    37621 gctgatacta aatcaataca tccccaagtc gtatagccta atagatcgac tccatctaat
    37681 tctactgcat ctttcattgc ctggatatgt gctgccagat agtcgattcg ataatcatct
    37741 tccacataac cattctcatc gggcgtatcg actgcaccta agccgttttc tacaataaat
    37801 agtggttttt gataacgatc ataaatatca ttcatcgtta ttctcaagcc taatggatca
    37861 atttgccaac cccattcact tgcttccaga tatggatttt tgacagaagc aaagatattt
    37921 cctgcagtct gttccaagag ttcgttgtca gtagtcgaaa ctcgagaaga ataataagaa
    37981 aaggagataa aatcaacagt atgttctttc agcaatgcgg catccccagg ttccattttg
    38041 atctgaatgc catttcgttc catttctttc aatgcataag cgggatattc tcctcgtgac
    38101 tggacatcaa tgaagaaata attttctcga tctgactttc tagaagccca aacatcttca
    38161 ggattgcaag tgtatggata atatgctcct gctgccagca tacagcctac tttgttctcc
    38221 ggatctactt catgagcgat cttcgttgcg atggcacttg caaccaactc atgatgtgcc
    38281 gcttgatatt tgatttgttc ttcattttct ccttcttcaa aatacagccc tgcgcccatg
    38341 aatggtgcat gaaggatcat attgatctca ttgaatgtca accaatattt gacaagtcct
    38401 ttatagcgat tgaagatgac tcggcacaga ttttcataaa aaccaacgag ttctctggaa
    38461 cgccacgcac catattcttt gatcaaatgc atcggacaat caaaatgtgt aatcgtcaca
    38521 agcggctcga tcccgtactt ttgacattct ttaaagacat cctcataaaa tttcaaccca
    38581 gattcatttg gctctttttc atcgcccatt ggaaaaatac gactccaagc aatggacatc
    38641 cgataagtct tgaaccccat ttctgcaaat aaagcaatat cttccttata atgatgatac
    38701 atatcgatcg caatttttgc tggataaaaa tgattttcat caaattcaaa cattttcttt
    38761 tttccagtaa tgacaggaaa acggtcttct ccgattggca caacatctac gttcgctaac
    38821 ccacgtccgc cttcatcata accgccctcg cattgattgg ctgctgtagc tccgccccat
    38881 aaaaaatctt ttctgaatcc catcatactt cctccattac atgtgtattg cttgataatt
    38941 ctcttgccag ttcatcttgg acttcgattc cttctgcttt taaccggctg attgtttcct
    39001 tgaattgcgg taaatactgc tgatgtgcaa taaacaactc atccatgatc cgttttgctg
    39061 tctttcctga tcgtataagc ggattgatcg taaacgcttg caaagctgtt ccataatcac
    39121 cagtcacggc tgcttcgatc gtcagcaatt ccatattctt cattacttgc aaccagcctc
    39181 tttcagcagc aggaagggaa ccaaacgcta catttcgtgc gccttgtgcg ccaacatagg
    39241 cagtgacttc tactacacaa tctgccggca gatcaggaac agctccatca ttctttgtag
    39301 ataccacgat ctgtgtttct ttatttccat agatcgaagc aatcgtttca caggcagcat
    39361 ctgaataata agcaccacct cgtttttgca attgttcagg tttatgatcc aattctggat
    39421 ctttgtataa ttcaaacagc tctgcttctg tttctttcac ttgctgcgct cttgtaccaa
    39481 tcgtttgata ttcttctaaa gcatgttcca gcatttcttc ttcacgatag taatagcggt
    39541 ggtagccaca agggatcatg ttcatttgtt ttaattgttc tttgaaaaac gggatgtcaa
    39601 agatattttt cggcaatcca ttgtcttcat cgtataaacg atcaatcaat tctgccgtgc
    39661 ggtcatttcc tttattatct gctactttgt gccaatggaa gtgatttaga ccggcaaact
    39721 tgtagatcag ctcttctttt ttcttgccaa gcattgttgg ttctgtcatc atcgccatta
    39781 ctggcacatt gcataatccg atcactttat cccatttgcc atatctgata actgcttctg
    39841 tcaccatccc gcttgggttc gcgaaattaa tcagccaagc atttggacat aaacgcttca
    39901 tgtcttcaac gattgccaaa accactggaa cggtacgaaa agctttgaat atcccgccag
    39961 caccatttgt ttcttgccca atcatcccat aataactagg gatccgctcg tctttgattc
    40021 gtgcatctaa ttgaccgaca cgaaactgag tcgtaacaaa atccgcatct ttcaatgctt
    40081 cttcccgatc cagagttgca tagattttga cttcatacgg agaagcatcc cacatacgct
    40141 gagacatctg actgacgatc tcaagctttt cttttccttc ttctacatct acgagccaga
    40201 tttctcgtat cggtaattcc tcatatcttt tgataaatcc ttccatcaac tcgggggtgt
    40261 aactgctgcc tccaccaatc gtagcgattt tgattccttt tgccatgttc tcactccttt
    40321 actttttaca cttttattat cttttttgta aacaaaacaa acaatagaaa ttcgtttctt
    40381 tccagtaaca tttacaaaag ttagtgtttt cttctggatt tttgtaaata tcttgtcaaa
    40441 aaatgtcttt attaagctat ataacataaa tccgtttaca aatcattttc aaaaaagtta
    40501 taatttacgt aaaaaaacaa gaagaaggta tctacatgtt attacgtgaa aaaatcaaag
    40561 aaatctcctt ttctccttca gaaaatgaag tgattcagtt tctcttaagc gattctccag
    40621 cacttggcga aatgaccatc caagagattg ctgagcgatg ttatgtccac ccgtccacac
    40681 tcatccgtgt agcaaaaaaa atgggttttg atggctgggt cagttttcgt aatgcttttt
    40741 tagctgaaac agcctatctt gaagggaatt ttcaagacgt agatgcgaat cttccttttt
    40801 ccaccacaga cggtattatg acgatcgcaa ataaaatggc ttctttagaa cagatgacga
    40861 ttcaagatac actttctctc attcatcatg accaactgca aaaagccaaa caactaatgc
    40921 ttcaagcaaa aaaaatcgtg attttcgcca gtaactcgaa tactttgatt tctcaagatt
    40981 tcatgctgaa aatgaagcgc atcaaaatgg atgtcacgat tgtcaccact tttggtgaat
    41041 cgttctatga agcgtacaat tgcacaagtg atatgtgtgg gattctgatt tcttatacgg
    41101 gtgaaaacaa tgtgatcctt cgtaccgcag aaatattgaa acaaaatcat gtgccaattt
    41161 tagctttgac cagtatcggt gattcaacgc tatcttcaat gagtacagcc gctttgcgac
    41221 ttacgacacg cgaacgactg tattcgaaaa ttggtaattt tacgatcaat acatcgattt
    41281 gttatcttct agacgtgtta tatagttgtg tttttgcaga aaattatcaa cagaatttag
    41341 tccatctaat ttctgtagga caaaaatttg ataaacgccc tatcagctca gccatcatga
    41401 aagagccttt caattttgaa aataaagaag tggactaaaa agaatagtta agcctagtcc
    41461 attctttcac ctaaccgttc aggtcagtgc ggtgataagt gcatcgtctg gcccgaccgt
    41521 ttccccttga ttttccaaaa tatccaaata atccatgtaa ttgatgacaa tcacaggtgt
    41581 ttctaaactg tatccttcct cttcgatttt ctttttgtcg aagcgaagca acagatcgcc
    41641 ttgtttcact ctttctcctt gctgcaccaa gctttcaaaa tatttccctt ccaactgaac
    41701 agtatccaat ccgacatgaa tcagcaattc cagacctgct tcagaaacca tcccgattgc
    41761 atgtttcgtc ggaaacaggg acatgactgt tccgtcaaaa ggggcacgta cttctccttt
    41821 ttcagggtag atcaagattc ctcgacctag tgttccttgt gcaaaagctg catcctttgc
    41881 tgcattcagt ggagcgattt gtccttgtat cggtgttttt acaatttctt tacctgatct
    41941 tttctgtcta tcatctgttt catctgcttc ctccttccaa aagaaaaaag tcagcgcaaa
    42001 ggataccacc atcgcaatgg cgatcccaat caatacatta atcacatctg ttaaatcacc
    42061 atctggtcta atatagttgg gaattgtaaa gatgcctagt ccaccttgat tccaagaagt
    42121 taaattcatt aacccactat acgcgccccc taacgcacca ccgacacagg taaaaataaa
    42181 tattttttta cgtggcaatg taaacccata aatagctggc tctgtaatcc ccacaatacc
    42241 agaaacaatg gctggtattg caattgcttt gtcttttgga tttctaagct taaagtagag
    42301 tgcggtaagt gctgcagttt gtccaaaaga cactactgca acaggtgtca taatattttg
    42361 ccaaccattc acagaaaatt gcatcaatcc cattggtacg attgcccaat ggagaccaaa
    42421 catcaccaga atttgccaga agaagcctac gattgctcca taaataactg gcgaaagtgc
    42481 taaaagtgtt tcaaatccag ccattaaaat atcagttgcg aatgtgaata ctggtccaac
    42541 gagtaaaaaa cctaaaggca ttgtaataag aacggtgaag aatggaacga agaaattcgc
    42601 gatcatctca ggtactaact ttttaattgg tttctgaatt tggctagcta acaacacaat
    42661 aaagataatg ggaatgacag aagaagcgta gttacgcgca atccagggaa tgccgaatac
    42721 tgtttgataa ataggtgctt caaaaatagt gccagaaaat atggtcgtta gtggtgcttc
    42781 tgctgcacca ctcaaagctt ccagcgacaa gctaggattc attaatgctg cgccaagcaa
    42841 catccctacg aattcattta ctttaaattt acgggcagct gttaaaccga taataacagg
    42901 cataaattgg aatacagaat caccgatatt atcaaacata acaaatgtgc cagaaccgcg
    42961 gtcaaacgcg cctaagaaga caaggaatgc tgtcagtcca cgaatcatcc cgtcagcagc
    43021 taggatagca aggaatggtt ggaaacagcc cgatattcca tcaataagca tatcaaacgg
    43081 tctcattttt tctgttgaaa cagcctcatc aaaagtaagg tttcctgctt ttataacctc
    43141 ttcaaacacg actggcacat gattcccgat gacaacttga tactgccctc ccgctcgcat
    43201 taccgttaca ataccgtcca tatttttcaa gatctcatca tttgcttttt cttcgttttt
    43261 taatttgaaa cgaagccttg tgatacaatg tgtcaaacca ttgatatttt ccttcccgcc
    43321 gacattttct acgatttttc tcgctaattc ttggtatttt cccatttcta ttccctccaa
    43381 tttattagtg gaaggtttag ccaactgaat gtcactaccc ttatttcata cgttcttgtt
    43441 aatttatcct ccttttttta tttatttaca acgattctca tgcactaaac gcgcagtatg
    43501 gatcgttaaa tacatttttt cgtccgtgga catctggtag ttgaacgtct cggctaaaaa
    43561 gtctgctatt ttctcaacgc aaccaaacgt atccttatat tttttttgaa taacctgaaa
    43621 caattcctca gaaacttctc ccacttctga tttttctcca tctaggacgc gtgtcagaaa
    43681 aaaacgtaag tgtgtgacga atcgaaaaaa atggactgaa tcttcatcaa atgtaatctt
    43741 atagtaatag cggacgatat ttagaatatc ctgtattgtt atagtgaaca ggtaggtatt
    43801 gtcttgttgg ctttgaatgc cttgtgcatt gactaaatgc aatgcgataa atccggcttc
    43861 atcttctgat aacttgatgt ggtatttatc cgagattttc ttcactgttt tttttcccga
    43921 tggcatactc tttaggaaat agttgtttga tatcatacag caatacattt ggtacaaata
    43981 ttcccatttt gtgacgttcg atagccgtat acaaatgatc cgtcaatgca atatacacag
    44041 aatcattcaa ctggacttgt agagcctctt ttgtatattc aatgatttca caagaaattt
    44101 ctaagtactc aagaggaatc gcttgtgcca attcttggaa ctgttgtgaa agcccgtcat
    44161 ttgtcaactg aaattttttg tcgatgcatg agtcaaaaat cgggtcaccc actttctttt
    44221 tgaatgccaa accttttccc atcacgatca tttcctgatt tctttcatcc ctggttatta
    44281 cgacgttgtt attcagtact ttctcaatca gcatgttttg ctcctttcca tgatatatag
    44341 ggctgtatcc aaaacaaaaa gaaaaaccca aaaagccgtc catggaacca tgggagactc
    44401 atcaggttta gcttgcatca ggcaatcact atccaagttg tatcgttttc tttatattat
    44461 cgaaagcaaa cgcttttgtc aacgctttct tagtggttat tagccgcttt tgactagctg
    44521 tcgtcgtctt ttttaaatcg ggtgactacg ccactaacgc tcacagatga agaatctgcg
    44581 aagctctgtt ttattttcac tctagcgctt agggcaaaat gctactatgc tactgtcaca
    44641 acctcttttt acacaccact gctttgcagt ttgtacatat ctgcatacaa accattttgc
    44701 gctagaagtg tttcatgatt cccacgctct actatatgac ctttgtctaa aactaaaatc
    44761 aattctgcat cccgaatcgt ggacagccga tgggcaatcg ctatcgttgt ccgtccttgt
    44821 cgcatttttg ccaatccttc ttggatcaat ccttcggttt cagtatcgat atttgctgtt
    44881 gcttcatcca atactagtat tttaggatct gtcacgattg ttcgcgcaaa agtgatcagc
    44941 tgtcgttgtc cactagaata gctggcaccg tgttcgatca cttttgagtg atatcctttt
    45001 ggtaattcat cgatgaactt gtttgcttga acaaactcag ctgcatggcg aatctgttcg
    45061 tctgtgatag ctggattcat catccggata tttcctgcaa tatctccata aaacaagaag
    45121 gcatcttgca agaccaatcc cattttctta cgtaactctt ccattggata ttctcgaatg
    45181 tcatgatcat caatcaagat ttggccttca taaaattcgt aaaaccgcat caatacgttg
    45241 atgatcgagc tttttccgct tccggtgtgc cccactaatg caacggtctc acccggatta
    45301 gcgacaaaag agatattgtt caatacattg tgctctccat cataagaaaa gcttacattg
    45361 cgaaactcga tcttacctga agtaatcgct tgatctgctc caggattttg ttgcggtgca
    45421 tattcttctg tatccatgat tttcaaaata cgactaccgg caaccatccc atctgtaaag
    45481 gtgctcaaaa agtccatcat ttgagacatt ggattaaaaa attcctggac ataggtcgta
    45541 aatgcataga tcagtccaac ttctacggga gaatgcaacg catcgatccc aaacaaagtc
    45601 aacgccaacg caatcgccaa tgcatagaga aaattgatga ttggacttaa cagaatcgaa
    45661 ttcgttttga tcatcgcata gcgcgtgtct aaatactcgt tgttcacttg ttcaaattct
    45721 ttagctaaac gagcttcttg gcgaaactgc tggataatac gcatgccgga aatcgattca
    45781 ttcaatttcg tgttcagctg acttaatttt tcccgcatat ttcggtaaat acgagaactg
    45841 aatttctggt aataccagat gacgattcct aaaatcggca agaacaccaa agtccacaag
    45901 gcaatttctc cattgatttg aaacatggca acgaaagaag aaacgactgc aaagatccca
    45961 gtcaatacca tcaaaaagac ataccaaaat tcaaatagcg tttctgtgtc attcgtcact
    46021 cgagaaacgg tcgatcctgc cggtgtctgg tcaaaataac gcatacctag tgtatgcagc
    46081 ttttcaaata atcttacccg aacatactga taggtcttca atgaagccat ggaatacaaa
    46141 taccattgga aaaaccaaat gatacttttg actatgactc caaacaagta cacaccagca
    46201 aaaaacaaaa taacttgtgt cgtagctgac tgtttagcca gatgatcatc cataaaagtt
    46261 tggatgatcc ttggcaatag gacattgatc acagacaggg caaacgcaaa taagatagca
    46321 acgatgaacg ttcttctaaa aggtttggcg aacttcatca gtcgtttgat gatcttcttc
    46381 tgttctttaa gtggaatcga ttttgtccat tccgattgat aattttcatc catcaggcct
    46441 cgcctccttc tatcttcgct tctaattgct gtttttccca cattcgttca taccagccac
    46501 ctaatgcaag cagctcgtgg tgggttcctc gttcaatgat tttcccttga tcgacaacaa
    46561 tgatttcttt tgcatgcatc acgctgctta aacggtgagc tgcaatgatg gttgttttct
    46621 cttttcttat ttcttttaaa ttatgcaaga tagcttcttc tgttttggca tctactgcag
    46681 aaagtgcatc atctaagatc agcaattcag gatttttgat caatgctcga gcaatcgaaa
    46741 tcctctgttt ctgtccgcca gataaagaga ctccacgttc tccaactaag gtatcataac
    46801 cttttggcaa ttccttgatt tcattgttga taaatgctaa agaagccgca tgctctactt
    46861 cttcttctga aagttttgga ttagaaaaac ggatattatc tcgaatggtc atcgaaaaca
    46921 ggaagtgatc ctgaggcaca taaccgattg atcctaataa agcattcaat gaatattctt
    46981 tgatcgaacg tccgccaaat tggattttcc cttgataatc atcgtattca cgcatcagta
    47041 atttgatgat tgtcgttttt cccgcaccag ttttcccaac aatacctaat gtttctcctt
    47101 cttggatcgc aaatttgatt tgttctaatg ctggtgattc ttctttcgga tagttaaaag
    47161 aggaaatagc tacattcagc tcgccttttg ccggggtctg cactgcaccc agttgttcca
    47221 cgatatgcgt cttttcatgc aaaagttcat tgacgcgatc ataagaagca tttcctcttt
    47281 ccaatacgtt gaacaatcga ccaatcgcaa acatcggcca aacaagcata ccgatatagc
    47341 taataaatga gaccatctgc ccgattgaga tcgtgccttc caagacatac tttccaccta
    47401 aaataattgt tgcaacataa gacagaccga taatcaaggt aatgaatgga tcaaataagg
    47461 agtctaaaaa attgactcgt ttgtttttaa taattgcatc ttcaatcttt tcttcaaaat
    47521 ccgcgagatc ttctttttct tgtccaaatg ttttaatgac tttgatccca gtgatacttt
    47581 cttgtgtctt atcattgata ctagaaaaag cttcttgcga atcacgaaaa gcatcgtgga
    47641 gttttgaccc cagcactcgt gaagtaacgg ctagcaaagg cagaggaatc agcgcaatca
    47701 gtgttaagcg ccaatcaata aacaaaatca tcgcaatgat cgttgttccg cccgtaatca
    47761 ctgaatctgc aaaggtcaag attccagctc ctgctacatt ctggattgca ttcaaatcat
    47821 tcgttgcatg agccatcaaa tctccagtcc gatatttttg gtaaaagaga ttgtccattt
    47881 ctgtaaaatg tttgaatagc tgctgccgaa gttctctctc caaccgagct gcacttcccc
    47941 aaatattcgt ccgccagata taacgaaaaa gatattgtaa gaaagctgca aggatcaata
    48001 ataagatcca ccaggaaatg atctttattg ataaattctt atcggcgatc tcatcaatga
    48061 tgatcccaat cacttttgga ggaactaact gtaccaaggc cactgcgaat agggagaaaa
    48121 tcccaattaa ataatgtttc ttttcctgac gaaaaaacca acctaatttt ctaaaaatcg
    48181 acattttact cctcctttgt ctttatcaag agcataggat tgtctgtgct tgtctttttt
    48241 gctgaatagt ctgaatagtc taacaaatta cgaaataaaa aacggactga aaaaatcagc
    48301 ccgcaaaaag tctttgggtc agaacctatt ttttcccttg gttcttcatt tgctgcatca
    48361 tctggcgaat tttcttctca gatggttttt gccccattga catcatcatt gatcgcaaca
    48421 tgtcctcatt aacgggagga tttttttcta aatagtcttt catatattta cgcgctagga
    48481 aaaagccacc tactgcgccg attagcaaag caataatagc aataagtacg acaatacctg
    48541 ttgataccat ataaatctct cctttctatt tccgaattgc tctcaatgat ttattttact
    48601 aaagtttatt gaaaaaagaa aggcgtattc tttagtttca tgcaaaaaat agtggttttt
    48661 ttagagaaga acaaaatttc tttcattaac cagcaagaca caccacgaaa aaaaattagg
    48721 cataccgaat ctttttcttt aattttttct ttctgaggtg tagaatgtaa tcgtagataa
    48781 ataggaaagg tggttttttc ttgaataatc aatccaacaa acaatttcat aagcatcgac
    48841 ttattgaaca tacaaatggt ttatctttag ctgaagtaaa tggaacagtg gatgtaccaa
    48901 agggaaaaag tttttttaaa acacttcttg cttactctgg accaggtgca ttagtagcag
    48961 ttggttatat ggatccaggt aactggtcca cttcgatcac tggtggacaa aatttccaat
    49021 atttattgat gtcagttatt ttgatgtcta gtctaattgc tatgctttta cagtatatgg
    49081 cagcaaaatt aggaatcgtg acccaaatgg atttggccca agcaatccga gcacggacaa
    49141 gtaagacatt gggtgtcatt ttatggatct tgactgaatt agccattatg gcaacagaca
    49201 tcgcagaagt cattggtgca gcaatcgccc tctacttact ttttgatatt ccattggtga
    49261 ttgccgtctt tattactgta ttagatgttt tcgttctctt actactgaca aaagtcgggt
    49321 ttcgtaagat cgaagcactg gttgtctgct taattttcgt cattttattc gtttttgtct
    49381 atcaagttgg cttgtctaac ccagattggc acagtttatt tgctggcttt atccctaaca
    49441 gtcatacatt ctcagactca ccggtcatcg gcggcgaaac tccgctgact ggagctttag
    49501 ggattattgg tgcaacagtc atgccgcata acttatatct tcattcagca gtttcacaaa
    49561 ctcggaagat cgatcaccaa aacgaagaag atgtagcaca agctgtacgt ttttctactt
    49621 gggattctaa tatccagttg tcaatggctt ttctagtcaa tgcactgcta ttgatcatgg
    49681 gtgtcgctgt cttcaaaacc ggttctgtcc aagatccttc cttttttggt ctgtatgaag
    49741 ctttatctga tccatcagct atgagcaatg gtattttgat tgctgtagca aaatctggta
    49801 tcttgtccac tttatttgct gtagcacttc ttgcttctgg ccagaactct actattactg
    49861 gtacgttgac cggtcaagtc atcatggaag gattcatcca tatgcgtatc ccgatttggc
    49921 taagaagact ggtcacacga ttgctttctg tgattcctgt attgatttgt gttttgttta
    49981 caagtacaaa aggaacgatc gaagaacatg ccgctttgaa taacttaatg aatgaatccc
    50041 aggtgttctt ggcttttgcg ttgccatttt cgatgattcc tttactgatg atgaccaaca
    50101 gccaagcgga gatgggtagc cgatttaaaa attcattcat cgtaaaactt cttggctggt
    50161 tctctgtgat tagtctaact tacttgaact tacgaggatt accaggacag atcgaaagtt
    50221 tcttcggcga gcaagccact tccgctcaac tagcattggc tgatcacatc gcttatgtaa
    50281 tcattgttct tgtccttctc ctgttggcat ggacgattgg tgatttatat aaaggaaaca
    50341 aaaaatatga acagcttttg caggaaacgg tagaagaaat cgaataaaaa ctgatttttc
    50401 cacacaaaga cccttgatag ttaggttaaa aatctagcta ccaagggtct ttttatttag
    50461 aagataaagc ttttttcaga tttaatttac tgaaaactag catgcttgtt actacgatga
    50521 attgcgttaa tggcacgcct gcaccagtgt agacacttaa aaaatagtcc tttttttctg
    50581 gcgcataaga acaaaaccat gagacaccga aaagaaaaac caattgtgtc agtattaatc
    50641 cggtgaccac aatcaaagag gaactataat aggcacgatc agcttttgca aatacggttc
    50701 ctagtatcat aagtaatatc cctgctattg aagcaatcag cacgggaata gccagaactt
    50761 ggtaaatttt tatgatgaca ttcgcgactt ttgctgattt tgttaattgg aaattaggtg
    50821 ttctcggttc attactatcg atccaattct ccattagcgg aaggtttaat aattcttgag
    50881 cctgtttgac ttgttcttgc gttccgaaac tataatttcc tccttgatgg aaatctttat
    50941 atcgccatgc ataagcgtat cctgattgaa ggaaatcgaa tacgattggc agatcactca
    51001 tcgtctttcc atctcctgat cccatcagat agaactcttt tttctttttg atggttccct
    51061 tgtgataggc ttcttctaat tcttggttga cttttttcca aaaatcattg gtttcttttg
    51121 cattgttttg gtaataacca gattcgctta ccacatctcg caatgcccaa aagatgatat
    51181 ctccgggtaa ttcttttact tttccacctt gagtccacgc gtgagtcgta tataattcgt
    51241 caatacgggt agaaacagta tggaatgtag gagatgcggc taatgcttta tttagtgcag
    51301 catgtgagac ccatatctca ctgttttctt tattcaagtt cgtttcatca tctattcgga
    51361 tcagttgttt catcaaacgt ccaaattctc cactggaacg gtcattgatg gttgcaatac
    51421 cgtatgttgt ctcattttta tattcgatcc actgatagac gatggaaaag ccaactatag
    51481 gaagtaaaaa caatgaaagt ttcagccatt cccgtttcga gagaagaaag agtcgttttt
    51541 ttaacttctt tacttctttg aaagaaaagt cttgccctag caagatctgg acgaaactga
    51601 tacccaaagc tactacagta aaaggaagaa gccagataga atcttcgcga atgaaccaaa
    51661 agaaaggaaa agcgacagaa agccctaaaa accatggcag caattttctg ctagaaccgt
    51721 atttgcggta ataaatagct aataaacaag cgataatgat ctcaacagcc ggaatcacga
    51781 gggcattacg atatgttcga gtggaatatt catgtgtaaa actaatcgga gaaaacaaaa
    51841 aataaaaata gatacttaaa agaagatact tgttcttcac aatcggttta aacgcgagtg
    51901 caaccaatat actggcaaaa atactgaaca tgccgaacaa aactgcataa gaaagatgca
    51961 gtttatgtgc aagtactaaa aataagctgt aggagattcc tttggaaagt gttgtagtcg
    52021 tatactcccc tagccattta ccatccaaaa tattttgagc atagtgaatc agcagctgat
    52081 catcataggt cgccgacata tctgttccat agattgcttt actatataaa tacaaccgaa
    52141 taattgtaaa taatacggct atgccaagaa taaagtatgt atgtttcttt tttagttggt
    52201 cgatcaaggg gtttaaaatg ttcagcatct tttcgtcctt tcaatcacgt tttgctactt
    52261 gtttgtgaaa cttttcgttt tttacttttc tatcagtata ccctgtttgt ctgaatttaa
    52321 aaagaggggg caaaaggagc atggcgaaaa cgaatgcaaa aagcactcct ttgtttctta
    52381 aacaaaaaag gacaaggcag actgcccaat ccttttttgt tgctgtcagg tagaaaactt
    52441 aaagtgtgac ttcttctgct aataactcag ggaacacttc gggatcgaat gcttgcgctt
    52501 tgaacatgga aatttctttt ccatatggtg cgtaagcttt ctttttatct ttaccgacat
    52561 atggggtttc taatattttc gaaatgttgt caaacattgg atcatggacg attttgttca
    52621 atgtgtcaaa tccaattgtt ccaaaaccga tattggcatg ccgatcttta tgagaacctt
    52681 gtggattttt agaatcattg atatgcagta ctttaagccg atctaatccg atgaccttat
    52741 caaactcttc cattacacct gaaaaatctt cttttatatt atatccggca tcattcgtat
    52801 gacatgtatc aaaagtaacg gataattttt cattcaattt tactccatcg atgatttgcg
    52861 ctaattcctc aaatgttcga ccgatttccg tacctttccc tgccatcgtc tccaaagcga
    52921 tctgtgccgt ctgttctttt gtcaaaactt cattcaaccc ctcaataatt ctagcaatcc
    52981 cagcttctgc ccctgctcct acatgagcgc ctgggtgaag ggtgatttga gtagcaccta
    53041 aagcttccgc acggacaatc tcttctctca gaaattgtac agcaaatgga aacatttctg
    53101 gtttgatcgt atttcccaaa ttaatgatat aaggcgcatg aacgacgata tttgttagtc
    53161 cgttttctgc cataaatgct tttccggcat caatattcat ctcttcgata gcttttcttc
    53221 tagtattttg aggagcgcct gtatagatca taaaggtcga agatccgtaa ctggctgctt
    53281 cttctgccgc gcctaacagc attttctttc cgctcatgct gacatgtgag cctaataaca
    53341 tggatggatt cctccttgaa aggcaattgt ttcgaacaat tcctgtatat tgataagatg
    53401 atgtacaaag aaaatggttt gtttttcttt gccctgttct ccatagtaac tgaaactttc
    53461 cctaaagaaa agcttaagaa ctctttcata aaaaagatca tttcctcacg ttttatctca
    53521 ttcgaaaaag aaattcctgt caaaaacagc cttgtctgtt tttttgtcct attatttgct
    53581 ataattagcg aaataaaacc attagttgag gtgacccttt tgccacgcaa aaatactcga
    53641 aaaaaacaga atagaagaaa aaaagaaaaa tggtttgttc ctaagattat ttttagagtg
    53701 tttcaatcat tgactgtttt tattactgtt cttcttttgt tgttcgcagc tctaggtatc
    53761 ggaataggtg ctggctactt tgcctatctt gtcgaagaca cacaattgcc gactaaaaaa
    53821 gaactgcaga cagaactagg aaacattacg gaaacgtcaa agattgttta tgcagataat
    53881 acagagatat caaaaatcca gacagatctg atgcggacga ctatttcatc tgataagatt
    53941 tcgcctttat tgaaaaccgc gattatcagt acagaagatg aatacttcga taaacatcaa
    54001 ggttatgtcc ctaaagctgt tttacgagca ctagtttcag aagcgactgg catcggctcg
    54061 tctggaggat ccactttgac acagcaatta gtcaaacaac agatcctaac cgatgaaaca
    54121 acattcaaac gtaaagcaaa cgagatcgtt ctttctgcac aagtagaaaa acacttttca
    54181 aaaaatgaga tcattactac gtatttgaat gtctcaccat ttggtcggaa taataagggg
    54241 caaaatattg ctggagtcca ggaagctgct agaggaatct ttggtgtaga tgcaaaggat
    54301 gtgactctgc cacaagctgc ttatatcgct ggtttgcctc aaagccctat cacgtatagt
    54361 ccatacacga atacaggcgc gctaaaagaa gatctttctg ccggtcttgc cagaaaagat
    54421 tttgttttat tcagtatgta ccgtgaagga caaattacga aagaacaata cgaagaagcg
    54481 aaagcctatg atcttacaaa agactttctc ccacaacaaa tcgcggaaca gactgacaga
    54541 gaattcttgt attataccgt gatgaacgaa gcaacaagga ttattgcaca acaattggca
    54601 gaaaaagata atgctgatat gtcagattca tcggtcagtg aagcttatta tcaaaaagca
    54661 cagcaaacga tccaaaacaa aggatatacg atccattcga cgatcgataa agatatctat
    54721 gctgcattac aaaccggcgt agaaaattat ggctacatgt tagatgatgg tgctggtagc
    54781 caagtggaga ctggaaatgt attgatggat aacagaaccg gacgtatcta tggcttcgtg
    54841 ggtggtcgga attacagcca gaatcaaaat aaccacgcct ttgatacaga acgtcaagct
    54901 ggttcatcca tcaaaccggt tcttgtttac ggtcctgcaa tcgatatggg acttgtcgga
    54961 tcagaatccc gtgtatcaga ttatgcaacg acttggcaag aaggaaaaaa tgctggtgag
    55021 aaaatcgtca atgcgaccaa tgaagggtcg aacaccttcc aaaccgtccg tgagtcactg
    55081 gaatggtcca acaatatccc agcttatcac ttatatcaag atgtgttgaa ttccggtgga
    55141 tcgaaacaat acgcttacga gcactacttg gcgaagatga actacccagc aaatgataat
    55201 tggggcgttg aatcggctcc tttagggaca gtagatgtaa cgacgcttca acagacaaat
    55261 ggattccagg ccttagcaaa tggtggagtc tacgaagaag gctatatcat tgattccatc
    55321 acagataatg caggtaatgt gatttataaa catgaaagta attctgtccg tatttattcc
    55381 gaagcaacgg catcgatcat gaatgacatg atgcgtagtg tgatcaatga aaaaatcact
    55441 acaccattca aagatgtcat ctcaagctta aacggaaatc taggaaatgc agattgggtc
    55501 ggaaaaactg gttctaccaa tgaataccga gattcatggc tgatcgtctc aactccatct
    55561 atcactatca gtagttgggc aggacacgat gacaatactg gaatggacag ccaagcaaga
    55621 atacgttcgg ctaattatct tgccaatcta atcaatcaag cttaccaagc aaaccctgag
    55681 attttcggca ctgatgagaa atttgactta tcatccgatg tgatgaagaa aaaagtatct
    55741 gggttcacag gacaaacagc tggaaaagtt actgtcaata aaaaatccat ccaaacgcca
    55801 ggaaaaacag tggagtcact ttgggcaaaa aatggtccga agaaaagcgc ctttaaattc
    55861 ggcgtaggcg gtactgatga aaactataca gattactgga acaccgcttc agcttatgct
    55921 agagcgaatc cagagaccaa agaatcagac gataaagatg attgatctaa tagattaatc
    55981 agaattaaag ggagctgcga caaaaatctt gtcacagctc cctttttccg aataaatggt
    56041 gttcaaaagc ttacatctac gatattaaga caaatttatg taaaaatgtg aaaaac
//
LOCUS       DACEUZ010000005        49058 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.005, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000005 DACEUZ010000000
VERSION     DACEUZ010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 49058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49058
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.005"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            4..816
                     /locus_tag="IXG44_000251"
     CDS             4..816
                     /locus_tag="IXG44_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HAQ1656995.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            813..1613
                     /locus_tag="IXG44_000252"
     CDS             813..1613
                     /locus_tag="IXG44_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HAQ1656996.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1774..3042
                     /gene="rseP"
                     /locus_tag="IXG44_000253"
     CDS             1774..3042
                     /gene="rseP"
                     /locus_tag="IXG44_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HAQ1656997.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3110..4819
                     /locus_tag="IXG44_000254"
     CDS             3110..4819
                     /locus_tag="IXG44_000254"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HAQ1656998.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            5031..9383
                     /locus_tag="IXG44_000255"
     CDS             5031..9383
                     /locus_tag="IXG44_000255"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HAQ1656999.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIQ
                     LGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSFIV
                     KKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYAPE
                     GEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGKKA
                     GVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAVKM
                     YKGNVIESFDEFIDPGHPLSRTTVDLTGITDEMVRGSKSEEEVLRMFLEFSKDTILVA
                     HNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSLEQ
                     HHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILAKN
                     QAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKGVE
                     EARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVATGN
                     VHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEIVV
                     ENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRLEK
                     ELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAPHY
                     YCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPDID
                     LNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSAEV
                     DRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHSIH
                     DNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIYSK
                     TGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDATLA
                     EVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVPDW
                     YIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMCKG
                     KDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFVIE
                     GDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGVLK
                     DLPDENQLSLFDML"
     gene            9527..10000
                     /gene="rimP"
                     /locus_tag="IXG44_000256"
     CDS             9527..10000
                     /gene="rimP"
                     /locus_tag="IXG44_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HAQ1657000.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10024..11199
                     /gene="nusA"
                     /locus_tag="IXG44_000257"
     CDS             10024..11199
                     /gene="nusA"
                     /locus_tag="IXG44_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HAQ1657001.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNSKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11221..11514
                     /locus_tag="IXG44_000258"
     CDS             11221..11514
                     /locus_tag="IXG44_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HAQ1657002.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11511..11822
                     /locus_tag="IXG44_000259"
     CDS             11511..11822
                     /locus_tag="IXG44_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HAQ1657003.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11835..14141
                     /gene="infB"
                     /locus_tag="IXG44_000260"
     CDS             11835..14141
                     /gene="infB"
                     /locus_tag="IXG44_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HAQ1657004.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSSDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14168..14515
                     /gene="rbfA"
                     /locus_tag="IXG44_000261"
     CDS             14168..14515
                     /gene="rbfA"
                     /locus_tag="IXG44_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HAQ1657005.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKKAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(14623..14844)
                     /locus_tag="IXG44_000262"
     CDS             complement(14623..14844)
                     /locus_tag="IXG44_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1657006.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYYRKKKVEFFTFLFLFFG"
     gene            15292..17654
                     /locus_tag="IXG44_000263"
                     /pseudo
     CDS             15292..17654
                     /locus_tag="IXG44_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
     gene            18022..18945
                     /gene="truB"
                     /locus_tag="IXG44_000264"
     CDS             18022..18945
                     /gene="truB"
                     /locus_tag="IXG44_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HAQ1657007.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            18949..19890
                     /gene="ribF"
                     /locus_tag="IXG44_000265"
     CDS             18949..19890
                     /gene="ribF"
                     /locus_tag="IXG44_000265"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HAQ1657008.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            20242..21027
                     /locus_tag="IXG44_000266"
     CDS             20242..21027
                     /locus_tag="IXG44_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ1657009.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            21040..21834
                     /locus_tag="IXG44_000267"
     CDS             21040..21834
                     /locus_tag="IXG44_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HAQ1657010.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKN"
     gene            complement(22014..22466)
                     /locus_tag="IXG44_000268"
     CDS             complement(22014..22466)
                     /locus_tag="IXG44_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1657011.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
     gene            complement(22931..23299)
                     /locus_tag="IXG44_000269"
     CDS             complement(22931..23299)
                     /locus_tag="IXG44_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chaperone"
                     /protein_id="HAQ1657012.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            23449..23931
                     /locus_tag="IXG44_000270"
     CDS             23449..23931
                     /locus_tag="IXG44_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HAQ1657013.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            23968..24978
                     /locus_tag="IXG44_000271"
     CDS             23968..24978
                     /locus_tag="IXG44_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HAQ1657014.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(25093..25806)
                     /locus_tag="IXG44_000272"
     CDS             complement(25093..25806)
                     /locus_tag="IXG44_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1657015.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(25996..26586)
                     /locus_tag="IXG44_000273"
     CDS             complement(25996..26586)
                     /locus_tag="IXG44_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HAQ1657016.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQNEKWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
     gene            27070..27297
                     /locus_tag="IXG44_000274"
     CDS             27070..27297
                     /locus_tag="IXG44_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HAQ1657017.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            27313..28479
                     /locus_tag="IXG44_000275"
     CDS             27313..28479
                     /locus_tag="IXG44_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HAQ1657018.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            28713..29756
                     /gene="hrcA"
                     /locus_tag="IXG44_000276"
     CDS             28713..29756
                     /gene="hrcA"
                     /locus_tag="IXG44_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HAQ1657019.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            29850..30413
                     /gene="grpE"
                     /locus_tag="IXG44_000277"
     CDS             29850..30413
                     /gene="grpE"
                     /locus_tag="IXG44_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HAQ1657020.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            30464..32293
                     /gene="dnaK"
                     /locus_tag="IXG44_000278"
     CDS             30464..32293
                     /gene="dnaK"
                     /locus_tag="IXG44_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HAQ1657021.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            32444..33610
                     /gene="dnaJ"
                     /locus_tag="IXG44_000279"
     CDS             32444..33610
                     /gene="dnaJ"
                     /locus_tag="IXG44_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HAQ1657022.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(33692..34357)
                     /locus_tag="IXG44_000280"
     CDS             complement(33692..34357)
                     /locus_tag="IXG44_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar diacid utilization regulator"
                     /protein_id="HAQ1657023.1"
                     /translation="MELDFSSPYYVVVGQLSDNQQLCQERINPLLELLERVFSYYGQA
                     LLTVHRQQIIILVNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSF
                     LHAKQVCEWIAFHQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLF
                     KKTLACFSKNNGSIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLL
                     VQT"
     gene            34333..34506
                     /locus_tag="IXG44_000281"
     CDS             34333..34506
                     /locus_tag="IXG44_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAQ1657024.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            complement(34551..35288)
                     /locus_tag="IXG44_000282"
     CDS             complement(34551..35288)
                     /locus_tag="IXG44_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ1657025.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(35290..36792)
                     /locus_tag="IXG44_000283"
     CDS             complement(35290..36792)
                     /locus_tag="IXG44_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ1657026.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            37051..39045
                     /gene="uvrB"
                     /locus_tag="IXG44_000284"
     CDS             37051..39045
                     /gene="uvrB"
                     /locus_tag="IXG44_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HAQ1657027.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            39055..41871
                     /gene="uvrA"
                     /locus_tag="IXG44_000285"
     CDS             39055..41871
                     /gene="uvrA"
                     /locus_tag="IXG44_000285"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HAQ1657028.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            42134..43399
                     /locus_tag="IXG44_000286"
     CDS             42134..43399
                     /locus_tag="IXG44_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HAQ1657029.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            43403..44134
                     /locus_tag="IXG44_000287"
     CDS             43403..44134
                     /locus_tag="IXG44_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HAQ1657030.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            44269..45153
                     /gene="rapZ"
                     /locus_tag="IXG44_000288"
     CDS             44269..45153
                     /gene="rapZ"
                     /locus_tag="IXG44_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HAQ1657031.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            45150..46148
                     /locus_tag="IXG44_000289"
     CDS             45150..46148
                     /locus_tag="IXG44_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HAQ1657032.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            46182..47117
                     /gene="whiA"
                     /locus_tag="IXG44_000290"
     CDS             46182..47117
                     /gene="whiA"
                     /locus_tag="IXG44_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HAQ1657033.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            47660..48310
                     /locus_tag="IXG44_000291"
     CDS             47660..48310
                     /locus_tag="IXG44_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1657034.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            48497..>49058
                     /locus_tag="IXG44_000292"
     CDS             48497..>49058
                     /locus_tag="IXG44_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HAQ1657035.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
BASE COUNT        16408 a         8404 c        10563 g        13683 t
ORIGIN      
        1 agaatgttac gtttttttcc gcaaaaaaac aaatatattc aagaaaaaag cgaatttcaa
       61 tttaataccg atggaccaat tcctcagcac attgccatca tcatggacgg caatggtcgt
      121 tgggcgcaaa accgtcgttt gccacgagtt gctggccata aagaaggcat ggaaacagta
      181 aagaaagtca caaaaaaagc atcaaggctt ggagtgaaag tgttgacgct ttatgccttt
      241 tcgactgaaa actggaagcg accaaaagat gaagtaagtt ttctcatgca actacctgtc
      301 gacttctttg atacgttcgt accagaattg attaaagaaa atgtcaaagt tcatgtcatg
      361 ggttatgaaa acgtattgcc agaacatacg caagatgctg taagacgtgc aattgagcaa
      421 acaaaaaaca atactggaat ggtattgaat tttgctttga attatggaag tagagcagag
      481 atcgtgactg ctgtccaaga aattgccgaa gaagtagcaa aaggagaaat acacgcagaa
      541 gaaatcgatg atgaattgat tgctaagcat ttaatgacag gtttcttgcc taaagagtta
      601 caagatcctg agctgatgat ccgtacaagt ggagaagagc gaattagtaa ttttctgtta
      661 tggcagatcg catatagtga gctgtatttt acaaaagcgc tttggcctga ttttgatggt
      721 gcgcatcttg aagaagcaat cgcctcttat caaaatcgag accgtcgatt cggcggtgta
      781 aaaaaaacag aagatgagaa aggggatcag tcatgaaaca acgtgtgatc acagcagctg
      841 tggcgctagt cttgtttatt ccgattatat ggatcggagg catagcggtt gagcttacag
      901 cagcaatctt agcggtagta ggagtttacg agctgttccg gatgaaagga ttgacactct
      961 taagttttga aggagtttta tccgccgttg gtgcagtgtt acttgttttg ccaaaagaaa
     1021 gatggttttt ctttcttcca gagaatgtaa gtacatttat gttattttat cttatagtta
     1081 tgattctttt aggaacatca gttatttcta aaaatacgta tacgattgat gaagcaggtt
     1141 ttcctgtgct tgtcagcttg tatgtgggaa taggtttcca gaattttgtc aatgcacgaa
     1201 cagaaagctt ggctgttttg atatttggtc tatttattgt ttgggcaact gatatcggag
     1261 catatatggt tggtcgacgt tatggtcagc ataagttaag tcctgatatt tcgcccaaca
     1321 aaacgattga aggtgcgctc ggtggtatcg gtagtgctgt tgtagtcgca tttctttatt
     1381 tattgctata cccagcaaaa gaactattca atcatggtat gtttgtcatg cttctgatga
     1441 cgattatttt ttcagcagtc ggacaatttg gtgatttggt cgaatctgca atcaaacgtc
     1501 attatgatgt gaaagactcg ggaaatatct taccaggcca tggtggaatc ttagaccgtt
     1561 tcgacagttt gctgtttgta tttcctatca tgcatttatt tggcattatt tagtaagtat
     1621 acctgcgttt ctttgtgaat ttcataaaag acgcaggttt ttatttttca ttatattgat
     1681 actattggtg tctttggtat actatggtaa aattatagtg ttttatcgtt gttttataaa
     1741 aaacacaaaa aacaagtaaa ggagaaaaaa gctatgaaaa cgattctgac atttatcatc
     1801 gtttttggta tattagtgat tgttcatgag tttggtcatt tcttctttgc gaaacgatca
     1861 ggaatcctcg tccgcgaatt tgctatcggt atgggtccga aaatttatgg gcatcaagca
     1921 aaagacggga caacatatac gcttcgccta ttacctattg gcggttatgt tcgaatggca
     1981 ggcaatgggg atgatgaaac agaaatggca ccaggaatgc ctctttctct tttactaaat
     2041 tctgacggca tcgtagagaa gataaattta agtaagaaaa tccaattgac aaatgctatc
     2101 ccgatggaat tgagccgtta tgatttagaa gatgagctga ctatcactgg ttatgtgaat
     2161 ggagacgaaa cagaagtagt aacctaccct gttgatcacg atgcgacgat tattgaaaac
     2221 gacggaacag aaatcagaat tgcgccaaaa gacgtacagt tccaatcggc taaattgtgg
     2281 cagcgtatgc tgaccaactt tgctggaccc atgaataact ttattttggc aatcgtgtta
     2341 ttcatcattc tggcatttat gcaaggtggt gttcaagtga cgaacactaa tcgcgtagga
     2401 gagatcatgc ctaatggagc tgcggcagaa gcaggcttaa aagaaaatga tgaggtagtc
     2461 agtgtggacg gaaaagaaat ccattcatgg aatgatctga caacagtcat tacgaaaaat
     2521 ccaggtaaaa cattagactt taagatcgaa cgagaaggtc aggtgcaatc tgtcgacgtg
     2581 acaccgaaat ctgtagagtc aaatggcgaa aaagttggcc aacttggtat aaaagcccca
     2641 atgaatacag gttttatgga taagatcatt ggagggacgc gtcaagcttt cagcggttca
     2701 ttggaaattt tcaaagcctt aggatcacta tttacaggtt tcagtttaga taaattaggc
     2761 ggacctgtca tgatgtacca attgtcttca gaagcagcaa atcaaggtgt aacaactgtt
     2821 attagtttga tggcgcttct ttcaatgaat ctcggaatcg tcaatctgct tccgattcct
     2881 gccttagatg gcgggaaatt agtcttgaat atttttgaag ggattcgcgg gaaaccatta
     2941 agtcaagaaa aagaaggaat attgacatta gctggctttg gatttttaat gttgttgatg
     3001 gtgctagtta catggaacga tattcaacga ttctttttct agaaaaagtt gacaaatagg
     3061 gtataataac aaacgataac tattttgagg aaataaagga gtatatagaa tgagacaatc
     3121 aaaaatgttg attccaacat tacgcgaagt tccaaatgat gcagaagtat taagccatca
     3181 aatcttgcta agagctggat atatccgcca agtatcagcg ggtatttatt catatctgcc
     3241 attagcaaat cgtgtattgg aaaaactgaa acgcattatg cgggaagaat ttgaaaaaat
     3301 cggagcggta gaaatgctga tgcctgccat cttgccagca gaactttggg aagaatccgg
     3361 gcgttacgaa acatatggac caaacttgta tcgtttcaaa gaccgcaatg atcgaaaaat
     3421 gatccttgga ccaactcacg aagaaacttt tactgatttg atgcgtaatg aaatcaattc
     3481 atataagaaa ttacctttaa atctttatca aatccaacct aaatatcgtg acgaaaaacg
     3541 acctcgcttc ggtttgcttc gcggaagaga gtttatcatg aaagatgcat attctttcca
     3601 tgcaacggaa gaaagtttgg acgagacata taaagattac gaaaaagcct ataccgaagt
     3661 attcaagcgt tgtggcttag aatttcgctc gatcatcgga gacggcggcg caatgggcgg
     3721 gaaagactca aaggaattca tggcgatctc agaaatcggg gaggatacga tctgttattc
     3781 gactgaaagt gattatgctg caaacttgga gatggctaca agctactatg tatcaaaaaa
     3841 atcgcatgaa acacagcttg aattggagaa agtagctaca cctaacgtaa aatcaattga
     3901 cgaagtagca gatttctttt ctgttgaacc tcaaaaaatc atcaagtctg tattgttcat
     3961 tgcggatgaa caacctgtcc ttgttttggt acgtggagac catgaagtca atgacgtaaa
     4021 actgaaaaac tttttatctg ctgacttttt agaagaagca acagaagaag aagcacaaaa
     4081 atatctaggt gctgagtttg gttctgtcgg accagtcggt gtttcagatg acgtaaaagt
     4141 atatgcagac cgttatgtcc aagatttagc gaatgccatt actggagcga acgaaacagg
     4201 attccactat aaaaatgtga atccagaacg cgatttcact gtgctttctt atgaagatct
     4261 tcgttttgta caagaaggag atccttcacc agacaacaac ggtgttcttg cctttactcg
     4321 aggaatcgaa atcggacata tattcaaaat cggcactcgt tacagtgaat ctatgggagc
     4381 aactgtacta gacgaaaatg gacgagaaaa atttgtgatt atgggctgtt atggtatcgg
     4441 tgtaagtcgt ttattatcag caatcgtaga acaacattca gatgaacaag gaatcaattg
     4501 gccaaaagga attgcgccat ttgatctgca tatcgtccaa atgaatctaa aagacgaata
     4561 ccaaacaagc ctgacgaatg aactagaaga agcgatgaca aaagctggct atgaagtact
     4621 ggttgatgac agaaatgaac gcgcaggagt gaaattcgcg gattctgatt tgattggctg
     4681 tccaatcaga atcactgtcg gtaaaaaagc agtggatgga atcgtagaag tgaaaatcaa
     4741 gaaaactggc gaaatggttg aagtacgtaa agaagaattg gcgaatactt taccaatcct
     4801 cttgaaccaa ggcgaataat aagcgttaaa acacagaaaa taaaaatagc tgaatcagct
     4861 gtttttgaca atctgatgat tcgcttttaa aattggaact gcgatttgaa tcgcagttcc
     4921 aattttttaa tgtaaaactc agaaaaaacg agtatactaa aaggtactga aaaagaagag
     4981 gataagatgc gatcaattgc attgtttctc atcagaaagg gcgtatatct ttgtcagaaa
     5041 aacgagattt atttgaaaaa ctgttggaac aaatccaact agaggaagca gaaaaaaatc
     5101 atccactgct tactgctggt gaaatggata gtgtagtggt ccatcgtaaa tcgcgtttat
     5161 gggaatttac gctacatttt tccaaaattt tgcctattat gctatatcgg tcattgactc
     5221 agcatctgac tatcgctttt aaagatattg cacaagtcaa actgaatatc ttagcagatg
     5281 accagacatt cgatgaacag ctcttgcaag attactgggc ccaagctttg gaaaatcagc
     5341 aatgcgatac accactagca cagcaagtat tgaaaacgca ggtacctgtg ataaaagatc
     5401 gtaaagtcat cttgccaatc gacagtacag gagctatttc ttatttgaag caacaatact
     5461 tgccattagt agaagagttg ttcgtcagtt acggattccc aaaatttcgt atcgaaccag
     5521 aagtggatga gcagcaagcg gaacgtgtat tgaaactttt tgaagaacgc aaacaagaac
     5581 aggcagaagc ctttatgaag caagcagctg aaagtctagt tgtccacgaa caaaagaaaa
     5641 aggaaagaaa agagcaatcg ccagctcttg aaggacccat ccagctagga cgaaatattc
     5701 cagccgatga accaacgaca ccaatgatca gtatcgttga agaagaacga agagtcacac
     5761 ttgaggggta cgtattcgac aaagaagtca gagaactacg ttcaaaacgc aagatcttga
     5821 ctttgaaaat cacagattat acttcatcat ttatcgtcaa aaaattctcg aacggcgaaa
     5881 aagatgaaca agtatttgat gcgatcagtg tcggtagctg gttaaaggta cgaggaagta
     5941 ttcaagaaga tacgtttgtc cgtgatttgg tcatgaatgc gcaagatatc atagaagtca
     6001 aacatacacc tcgcaaagat tatgctcctg aaggggaaaa acgagtagag cttcatgttc
     6061 atagcaatat gagtacgatg gatgcaacaa acagcatctc tgatcttgtg gcacaagcag
     6121 gaaaatgggg acaccgagct atcgccatta cagaccatgg tggtgcgcaa gcctttccgg
     6181 aagcacatag tgctggaaaa aaagcaggtg tgaaaatctt atacggcgtg gaagcgaatg
     6241 tcgtagatga tggtgtccca attgcttata atgatgcaca tgaagcattg agtgaagcca
     6301 catacgtcgt atttgacgtg gaaacgacag gattgtctgc agtatatgac acgatcatcg
     6361 agctagccgc agtcaaaatg tataaaggaa atgtgatcga aagctttgat gaatttatcg
     6421 atccaggaca tccattgtca cgaacaacgg tagatctgac tggtatcacg gatgaaatgg
     6481 ttcgagggtc aaagtcggaa gaagaagtat tgcggatgtt ccttgaattt tcaaaagata
     6541 caattttggt agcccacaac gcggcttttg atatggggtt cttgaatact agctacgcaa
     6601 ggtatggtat tcctgaagcg gccaatcctg tcattgatac gttggaatta gctagatatc
     6661 tgtatcctca attcaaacgt ttcggattag gtgtgttatc taaaaagttt ggtgttagct
     6721 tggaacagca tcaccgagca atctatgatg ctgaagcaac tggtcactta gcatggatct
     6781 ttgtgaaaga ggcaatggac aatcataaca tgctttatca tgatcagcta aatgagcata
     6841 tcggagaagg agattcttac aaaagggctc gacctttcca tgtaacgatt ttagcaaaaa
     6901 atcaggcagg actgaaagat ttgttcaaac tgatttcaat gtcgaatgtc gaatacttcg
     6961 aacgcgtccc acgaatacca cgctcccaat tgaaaaagat gcgtgaaaat ctattgatcg
     7021 gctcagcttg tgataaggga gaaatattcg aagccatgat gcaaaaaggg gtagaagagg
     7081 caagaaaccg agcaaaattc tatgattata ttgaagttat gccaaaagcg gtctatgcgc
     7141 ctttgatcga gcaagaattg gtaaaaaatg aacatgattt ggaagaaatt atccaaaatt
     7201 tggtagaaat cggaaaaagt ttggataaaa tcgttgttgc aacaggtaat gtacattatc
     7261 tgaacgaaga agatgccatt taccgaaaaa ttttgatcaa ttctatgggc ggagcgaatc
     7321 cgttgaatcg tcatagtttg ccggatgtcc atttccgaac aaccgatgag atgctaactg
     7381 catttcactt tttaggggaa gaaacagcga aagagatcgt agtagaaaat acgaataaaa
     7441 tcgcagatat ctgcgaagaa gtgattccag tcaaagatga actttataca ccgaagattc
     7501 caggttcaga agatgaaata tcagaattga gttacacaaa agcaaaacaa atgtacggag
     7561 atccgttacc tgagatcatt caaaaacgat tagaaaaaga gttgaattct attaatggta
     7621 atggcttctc ggttatttat ctgatcgccc aaaaactagt gcataaaagt aatgaagatg
     7681 gctatctagt aggttcacgt ggctctgttg gctctagctt tgttgcgacg atgacaggaa
     7741 tcactgaagt taatccatta gcccctcatt attattgtcc agagtgccaa tattccgagt
     7801 tttttgaaga tggtacgtat ggttctggat ttgacatgcc agaaaaacaa tgtccaaaat
     7861 gtggtgcacg cttaaacaaa gacggtcatg atattccatt tgagacattt ttaggattcc
     7921 atggagacaa agtgcccgat attgatttga acttctcagg tgattatcaa gcagaagccc
     7981 ataactatac gaaagtattg tttggcgaag actatgttta ccgtgccgga acgattggta
     8041 cagtcgccga caaaacagca tatggctatg taaaaggata cgaaagggac aataatctgc
     8101 agtttcgaag tgcagaagtc gacagattag caaaaggcgc tactggagtc aaacggacaa
     8161 ccggccagca tccagggggg atcatcgtta ttcccgatta tatggatgtc tatgatttta
     8221 cgccgatcca atatccagca gacgatcaaa attcagaatg gaaaacgact cattttgatt
     8281 tccactcgat tcacgataat gtattgaaat tagatattct agggcatgat gacccaactg
     8341 ttattcggat gcttcaagat ttatcaggga tcgacccaca aacgatccct acggatgacc
     8401 cggaagtgat gcggatattt gccggtcctg aagtacttgg agtaagtcag gaacaaattt
     8461 attccaaaac agggacatta ggtattccag aattcggcac tcgttttgtt cgagggatgt
     8521 tagaagaaac gcatcctaca acttttgcag aactactgca aatttccgga ctatctcatg
     8581 gtacagacgt atggttagga aatgcggaag aattgattcg acgaggagat gcgacactgg
     8641 cggaagtaat cggctgtcgt gatgatatta tggtttattt gatccatgct ggattagata
     8701 gcgggatggc atttaaaatc atggaaactg ttcgtaaagg acaatggaac aagatccctg
     8761 atgaattgcg agaaacctat ctatcggcga tgaaagaaaa taatgtgccc gactggtata
     8821 tcgattcttg ttcaaaaatc aaatatatgt ttccgaaagc ccatgctgca gcttacgtat
     8881 tgatggctct tcgtgtcgct tatttcaagg tttatttccc tattttgtat tactgtgcat
     8941 acttttctgt acgtgcagac gactttgatc tagtctccat gtgcaaagga aaagatgctg
     9001 tgaaacaagc aatgaaagaa atcacggaca agggattaga tgcttccgta aaagaaaaga
     9061 atcaactgac agtgctggag ttagccaatg aaatgctgga acgaggattc aaatttggaa
     9121 tgattgattt gtataaatcg gatgccgtta actttgttat tgaaggtgat acgttgatcg
     9181 caccatttag agctgtccca agtttgggaa ccaacgtggc gaaacaaatc gttgaagcaa
     9241 gaaaagacgg tccattttta tccaaagaag atcttgccac ccgcggtaaa gtttcaaaaa
     9301 cattgatcga atatatgaac gacaatggcg tattgaaaga tctaccagat gaaaatcaat
     9361 tatcgttatt cgatatgcta taagcatctg ttttcctgtc tgcttgtaat tttaagcaga
     9421 gtatggtata gttcttatag gtaatgatac taactgtgag tgagcggatt tttcgctcac
     9481 tctttttaat ggaattacgc acttaatttt taaggaggcg aaacttttga gtagcgtcgt
     9541 tgaaacagtc acagaaatgg tgacaccgat tttagatgaa caaaattttg aacttgtcga
     9601 agtcgaattt gtaaaagaag gaaaaagctg gttcttacga gtatttattg acaaagaagg
     9661 cggtatcgat atcgaagaat gcgcctttgt cagtgagaaa ctgagtgaaa aacttgatgc
     9721 agcagatcca gatccgattc ctcaagctta tttcctggag gtatcctccc cgggagcaga
     9781 gcggccattg aaaaaagaaa gcgactatga aaaggcactt ggtgattatg tccatgtctc
     9841 cttataccaa ccagtagatg gcgaaaaaca atatgaagga tttcttcaat catttgatgc
     9901 tgaacagctt acattgaaaa tacgcataaa aacacgagaa aaagaattga cttttgaccg
     9961 aaaaaatatt gcaaaagctc gcttagctat ccaattttaa tcacggagag gaatcagaaa
    10021 aaaatgagta aagaaatgtt aaatgcgtta gacgctttag aagctgaaaa agggatctca
    10081 aaagaaatcg tgatcgatgc attagaagca gcattggttt ctgcatacaa acgtcactat
    10141 ggccaagcac aaaacgtcga agtggaattt gatcagaaaa aaggaaagat ccatgtctat
    10201 gcggtaaaag aagtaacgga agaagtgatg gattcacagt tggaagtctc attgaaagat
    10261 gcacttttaa tcaatccagc atatgagatc ggtgatacga tccgttttga agtaacacca
    10321 aaagattttg gacggatcgc tgcgcaaacg gctaaacaag ttattttaca gcgtgtacgt
    10381 gaagcagaaa gaacgattat ttataacgaa ttcagtgctt atgaaaaaga catcatgcaa
    10441 ggaatcgtcg aacgccagga taaacgttat atttatgtca atcttggtaa aattgaggca
    10501 gttctttcta aacaagatca aatgccaaat gaattttatc aaccacatga tcgaatcaaa
    10561 gtttatgttt cccgagtaga aaatacgtct aaaggtccgc aagtatttgt tagccgcagc
    10621 catccagacc tattgcgtcg cttatttgaa caagaagtac cagaagtcta tgatggaatc
    10681 gttgaaattg tcagcatcgc ccgtgaagct ggcgatcgtt caaaagtggc agttcgctca
    10741 aatgatccaa atatcgatgc agtcggtaca tgtgtcgggc caaagggaca acgagttcaa
    10801 gcaatcgtca atgaattaaa aggcgaaaac atggatatcg tcgaatggaa tgaagatcca
    10861 gctgtattca tcgcaaatgc attgaatcct gcacaagtca tggatgtgat cttcgatgag
    10921 acaaattcaa aggcctgcac agttgtcgtt ccagactacc aattatcgct agcaatcggt
    10981 aaacgcggac aaaatgcacg tttagctgct aaattgacta accataaaat cgatatcaaa
    11041 tcagaatctg acatgacgga attttatgaa aatcaagaac aacaaaaaga aacagaagaa
    11101 ctgcatgatg aagcaatcgt tcagtcagat ttaacagacg atgaatatga aacaattgca
    11161 ttcaacaatg aaacggtcga agaaaaacca gaagcttaac tttagtaagg aggcaaagag
    11221 atgaaacaaa ggaaaattcc tttacgcaaa tccgtggttt caggtgaaat gaaacctaaa
    11281 aaagagatgg tccgaatcac ccgttcaaaa gaaggggaag tatcgatcga tccaactgga
    11341 aaaatgcctg gacgaggagc gtatgtctca cttgagccgg aagaagtaca acaagcttgg
    11401 gataaaaaaa ttctcgatcg agtactcgaa acaacattaa cagatgagtt ttatcaagaa
    11461 cttcttgact atgtcactca ccaaaaagca cgtaaagagt tatttggaaa atgaatacag
    11521 ttaatcggca aaaagccatg aatctcatcg gtctagcgat gcgcgcggga aaactaatca
    11581 cgggtgaaga actgaccatt gctgaaatcc gcaaacaaaa agcaaaattg gtgtttgtgg
    11641 caaccgatgc aagtgagaat acaagaaaaa agataaaaga taagagttca tattatgaag
    11701 ttccttgctt tgagctattt tctgaagcag aaatcacgca gatgatcgga aaacctcgca
    11761 aggtattcgg aataatggat gccggatttg cgaagaagac caaggagcta attgaaggtt
    11821 aggaaggtga ttgcatgggc aacaaaagaa tctatgaatt agcaaaagaa ctcaaaaaat
    11881 ctagtaaaga cgtcgtggaa aaagcacaac aacttggcat cgatgtgaaa aaccatatgg
    11941 gtgcgattac tagcagtgat gagaagaaat tgcaacaagc gttcaaaaac ccaagcacga
    12001 aacaacaagc tgcacaacaa gcaagcagaa aaccagccaa tcaacagccg aacgatcaac
    12061 gtaaaaaaga aacaacgaac aatcaacaaa aaaataaaaa taaccgcagt tatcaagatc
    12121 gcggtcaagg gagcggtcaa gtgaatcaag gaaaaaaacc aacaacaaac caaaataata
    12181 cgggaaatac cagcagccaa aatcgtcaag gaacgagcca aggcggcaat caaaaccgta
    12241 ctaacaataa caacaaacca aacaataaca atcgcggcaa atttaataat aataaccgta
    12301 atcgttataa taaaaaaggt aaaaaaggca aacagcaaca atcaactaaa ccagctgtac
    12361 caccacgcaa attccgtgaa ttgccagatg tattagagta tactgaaggt atgaacgtag
    12421 cagatattgc gaaaaaaatt catcgtgagc cagcagaaat cattaaaaaa ttatttatgc
    12481 ttggtgtcat ggtcaatcaa aaccaagcac tggataaaga tacgattgaa ctgttagcga
    12541 cggactatgg catggaacca caagaaaaag tccaagttga cattgctgat atcgataaat
    12601 tcttcgaggc agaagaagtc gatccagata aattagtctc acgtccgcca gtagtaacga
    12661 ttatgggaca cgtagaccac gggaaaacaa ccttattgga tacattgcgc cattcacgtg
    12721 taacaagcgg cgaagccggc ggaatcacac agcatatcgg tgcctaccag atcgatattg
    12781 atggtaaacc aatcactttc ttggatacac caggacatgc ggcctttaca agtatgcgtg
    12841 cacgtggagc aagtatcaca gatatcacga tcttagttgt tgctgcagat gatggcgtca
    12901 tgccacaaac agttgaagca atcaaccatg ccaaagctgc aggcgttcca atcatcgtcg
    12961 ctgtaaataa aattgataaa ccaggtgcaa atcctcagca tgttatgcaa gaattaagtg
    13021 agtatgaatt aattcctgaa gcatggggtg gagaaacgat ttttgttgag atctcagcga
    13081 aattcggcca aaacatcgaa gaattgctgg aaatgatcct gttagtcgca gaagtagaag
    13141 atttaaaagc agatccaaca caacgcgcaa tcggaacagt gatcgaagca cgtcttgata
    13201 aaggtaaagg tcctgtgtca accttacttg tccaagaagg aacacttcac gttggtgacc
    13261 caatcgttgt cggtaatact tacggacgtg tccgtgtaat gacaaacgat cttggccgtc
    13321 gcgataaaga agcaggacct gcaacgccag tagaaatcac tggcttgaat gatgtacctc
    13381 aagcaggtga ccgtttcgta gtcttcgaag acgaaaagac agcacgtgca gcgggtgaag
    13441 aacgcgcaaa acgtgcatta ttggaaaacc gtgctgccac aagccgtgtg actttggaca
    13501 acttatttga aagcctgaaa gaaggcgaat tgaaagaagt taacgtaatc atcaaagcag
    13561 atgtacaagg ttcagcagaa gcactggctg catcattgaa gaagattgat gtggaaggtg
    13621 tccgtgtaaa aatcgtccat tcagctgtcg gagcaatcaa tgaaagtgat gtaaccttag
    13681 cagcagccag caacgctatt atcatcggtt tcaacgttcg tcctacaccg caggcaaaag
    13741 tgcaagctga tactgaagaa gtcgatattc gtcttcaccg tatcatctat aaagccatcg
    13801 aagaaatcga aactgcgatg aaagggatgc tagaccctga atttgaagaa aaaatcaccg
    13861 gtcaaatgat cgttcgtgaa accttcaaag tttctaaagt tggtacgatt gcaggtgcct
    13921 atgtaacaga aggatatatt cgtagagata gcggtgtccg tgtaatccgt gacggaatcg
    13981 taatctatga aggccaactt gcaagtctga aacgattcaa agatgacgtg aaagaagtca
    14041 aaatgggtta tgaatgtggt gcgatgattg aaaaattcaa tgacatcaaa gtagatgacg
    14101 taattgaagg atttatcatg gaagaagtca aaaatgacta aaaactgata aaaggaggcc
    14161 aatcattatg gctaactacc gtgatcgccg agtcggtcaa gaaatcttaa aagaagtcaa
    14221 tgatattttg cgcaaaaagg tccgtgaccc tcgtgtggag aacgtaacga tcacagatgt
    14281 gaaagttacg ggggacttac agcaagcaac gatctattac agcattttat ctgacttagc
    14341 atcagataag aaaaaagcac aagatggatt gaataaagca tcaggattga ttcgtcgaga
    14401 gttagggcac aatctaagta tttataagac acctgaattg ttctttgaac ttgatgaatc
    14461 agttgtctat gggaaccata tcgatgaatt gcttcgcgac ttgaacaaag actaatcaaa
    14521 aaatcccgca aatcctgtga aatcaggggt tgcgggattt ttttgttttc attaagacgt
    14581 attacctaag agttccatga ctttagtctg gtagcgtgtc agctaaccaa aaaagagaaa
    14641 aagaaaagta aaaaattcga ctttcttttt tctatagtat ataaattttt gcatatagat
    14701 aaagatcaag ataccgataa cggaagctag attgtaatct attttagtga cagtcaggta
    14761 tagccaagta actagaagat cagtggaacc tgcaccataa aagtttgagt ttgtcggtcc
    14821 gttgacagtt aaaagaaaaa tcacgttgaa gttattgata tttccgatta cgccgcttcg
    14881 atttggtcac gatccaaatt ttggataatc cctgtagaaa ttagcatagt agctgggata
    14941 ccaattcaca tattgacaaa gataacagag acttttacat aaaacggatc aatcaggaaa
    15001 ggaattgctc gatccagccc cagttttcca acaatgtgtt aactggccta gcgtcattca
    15061 gcaaattatg catgatgagg agcgaaatga actgcggaac agccattgtg ataacgacaa
    15121 gttttttgga tcaatgtaaa aaacgtcagc gaaaggaaaa atttcgtggt tttttatacc
    15181 tgttcatttg gctagatata aaatccaaac aaatgaacag aagtgcttat taggtttcaa
    15241 ttgggagtat ctgcgctaga ataagagtaa taatgaaaag gagtgttatg tatggattat
    15301 tctagtaaag aatactttga taaaatgacg gcttggtggc gtgctgctaa ttatctttct
    15361 gttgggcaac tttatcttaa agataacccc ttactgagac gtacactgaa acccgaagat
    15421 gtcaaaaaac atccaatcgg tcattgggga acgattcctg gccaaaattt tatttatgtc
    15481 catttgaacc gagtcatcaa taaatatgat ttgaacatgt tttatattga agggccagga
    15541 cacggcggac aagtcatggt atcgaatgct tatttagatg gaagttacac agagatctat
    15601 ccagaagtaa cggaagatga gacaggaatg caaaaattat tcaaacgatt ttcatttcct
    15661 ggaggcattg cttctcatgc agcaccagaa acaccaggtt ctatccatga aggcggagag
    15721 cttggatact ctctttcaca tgcagtcgga gctgtattag acaatcctga agtaatctct
    15781 gctgtcgtga ttggtgatgg tgaagcagaa accggtcctt tagctggaag ctggttctca
    15841 aatgtcttta tcaatccagt gatagatgga gctgttttac cgatccttca tttaaatggt
    15901 gcaaaaatag ccaacccaac gatcttggca cgtaaatcag atggagaatt agcaaattac
    15961 tttaacggac ttggctggga acctttcttt attgaaggaa atgattcaga aaaattaaat
    16021 cctgttatgg ctgaaaaaat ggatcaagca atcgagaaaa ttaaatccat ccaaaaagaa
    16081 gctcgcctga agacagcaac agatgtcgtg atgccaaaat ggccagtatt gatcgtacgc
    16141 acaccaaaag gatggaccgg tccaaaagaa tgggacgggg aaccaattga aggaaccttc
    16201 cgagcacacc aagtaccgat tcctgtagat caagaacata tggatcatgc agatgcttta
    16261 ctccgttggc tgaaatccta tgaaccagaa aaattatttg atgcacaagg acgtatttta
    16321 gaagaaatca gagaaatcgc gcctactggt gaccaaagaa tggcaaaaaa tcctatcacc
    16381 aatggtggaa tcgatccaag accgcttatt atgccagatt ggaaaaaata tactttgcaa
    16441 tttgaaaaac caggctccgt taaagcagaa gatatgaccg aacttggtaa attcgttcgc
    16501 gagatcattg agaaaaatcc agaaaatttc cgtatatttg gaccagatga aacaaaatca
    16561 aaccgattga atcaagtttt caaaacgaca aatcgccaat ggatggaaaa aatcgaacca
    16621 gaaaatgatg aatggctttc gccatctggt cgcgtcatcg acagtcaatt atcggaacac
    16681 caagatgaag ggttcttaga agggtacgtt ctgactggac ggcatggttt tttgcaagtt
    16741 acgaatcatt cttgcgagtg gttgactcta tgttgactca gcacttcaaa tggatgcgga
    16801 aatcacatga cctttcttgg cgaaatgatt atccttcgct aaatctcatt gcaagctcaa
    16861 cagtcttcca gcaagatcac aatggatata gccaccaaga tccaggaata ttgacacatt
    16921 tggcagaaaa gaaggcggag ttcatccgtg aatatctgcc tgcggatgca aatacattgc
    16981 tcgcagtcat ggataaagca ttccgctcgt cagaaaaaat caatttgatc atctcttcca
    17041 aacatccgcg tgctcaattt tactcagcag aagaagcagc cgtcttagtg aatgaaggct
    17101 tgaaaatcat tgattgggct tcgacagcaa aagaggagga acctgaattg gtcattgctg
    17161 ctgcaggaac ggaatctaac ttagaagcat tagcagctgt aactttgctg ctggaagaat
    17221 tcccaaaact aaaaattcgg ttcatcaatg tcgtagattt attgaaatta cgtcatccaa
    17281 gtcaagatcc gcgtgggttg agcgatgaag aatttgatca atattttaca aaagacaaac
    17341 cgatcttatt tgctttccac ggttatgaaa cattggtccg cacgattttc tttgatcgtc
    17401 ataaccatca tttaatgatt catggctaca aagaaaatgg agatatcacg acaccatttg
    17461 acatgcgtgt agtaaatgaa cttgatcgtt atcatttggc aaaagacgca gctttaaaaa
    17521 tcaaaggaag tcaagcagaa gattttgcga aaaaaatgga tcagaaatta caagaacatc
    17581 aaaactatat tcgtgaaaat ggtattgatc tgccagaagt attggattgg aaatggaaaa
    17641 atcttgacca ataaaatgat aaaatcgatc ccgcaaaata gaatttttta cattgattaa
    17701 gaaactgaaa aaataaataa aaaaaacgga aaaaagactg gaagaatggc cagtcttttt
    17761 tttcgttttt gggaagtgaa aaccaaacta tttacctgta agccacccat aaatccaatc
    17821 aaatggtgca aagaaaaaat cttccattct attccctcct ttaaaaaagc ataacatgat
    17881 ttctatttac gtgagcatga attttgttac gtttttattg ccgaaaaata aaaaccgttc
    17941 attatgatgt caaattcgtc agtaattgat gcaatgtgtt ataataattc agttaaatca
    18001 aaaatgaaat ggagaaaaga catggacggg ttattacctt tatggaaaga acgcggaatg
    18061 acaagtcatg attgcgtatt caaattaaga aaaatcttac atacgaaaaa aatcggacat
    18121 gggggtacgc ttgacccaga cgtagacggt gtgttgccca tttgtattgg aaaagccaca
    18181 aaagttatcg aatatttgac agattctggg aaaatctatg taggcgagat aactctaggt
    18241 ttcagtacaa cgacggaaga tcgatccgga gaacgggtag aaacaaaacc agtagatatg
    18301 tctttatctg aaaaaaaaat agatgaagtg atggcttcat ttattggtga gatcacccag
    18361 ataccaccaa tgtactcagc ggtaaaagtc aacggtaagc ggttatatga atatgcccgc
    18421 aataatgaaa cagtggagcg tcctgttaga aaggctcaga tttaccgttt tatccggaca
    18481 agtgaattga caagagacga agtgacagga accatttctt ggcgcttcga agtagaatgc
    18541 gggaaaggaa cctatgtccg cacattagct gtagatacag gcaagaaact tggttaccct
    18601 gcgcacatgt ctgatctgac ccgaacagca agtgccggta tgtcccagaa ggatgctctg
    18661 acattagaag aagtagctca agctgtgaaa aatgggacaa tcgatgattg tttgctgcca
    18721 atagaaaaag gagtggaacg gttcaaacga gtagatatta ctgatgaagt atggcagaaa
    18781 gtaaaaaacg gtatgagact tgattatact gtatttgcgc tcccagaaat gccgtcagaa
    18841 gaaattgctt tgttttataa aggaaaagtt gtcagtattt atcagccaaa cccgaaagaa
    18901 aaaaacaaat taaaacctag taaagtattg agaaatgagg aataacgaat gaatatcatc
    18961 aagatcagac atccttacca atcagaacaa attccagaag acgatgtcgt tttagtgctg
    19021 ggatttttcg acggcgtgca tatcggacac cagaaagtca taaagacagg tatggaaatt
    19081 gcacaaaaag aaggattgaa attagcattg atgactttca atcagcatcc atctattgtc
    19141 tttaaaaaga tcgatcctag cagcgtgaaa tatttgacta gccttaagca aaaagaagaa
    19201 aaaatggccg ctttgggtat agattattta tatgaagttg attttacttc ttcttttgct
    19261 catttagcac cgcaggaatt tgttgatcaa tacatcgtag ggttacatgc aaaatatgct
    19321 gtttccggtt ttgattatac gtatggacca aaagacattg cagatgtcgc tcattttcct
    19381 gaatatgcca aaggaagatt caaaatcgtc acagtttcta aagaagaaga acaaggtgaa
    19441 aaaatcagct ctacccgtat tcgtggatta ctagacagcg gacaagtaaa agaagcagca
    19501 aaactattag gttgcacata tgaggtagaa ggggtagttg tccatggcga tgcacgaggc
    19561 agactactag gattccctac agcaaatgtc aaagtaaaga gcacggttca tttgcctaaa
    19621 gaaggtgttt atgtgtcgga aataaaaatt ggggaaacat ggtatccagc catgggttca
    19681 attggacata atgatacatt cggtgaagga cgggagctga ctgtagagtt gtatattttg
    19741 gactttaatc aagatatcta tggtgaacat gtcgcggtac gctggaatca atttttaaga
    19801 gatcaagtga aattttctag tgtagaggaa ttgattgaac aactcaagga ggatgaacaa
    19861 gcaacagctg aatatttttc tcagcaataa aacattatgc ataaaatctc ttgtttatgt
    19921 ataactagca attacaataa aatgggtgat aaataagccc aggaaagggg ctgtgacaaa
    19981 agtcttgtca cagccttttt ttccaaataa atggtttttc agaagtagct tcttcggaaa
    20041 taagccgaaa tttcttgaag ctgaccactt ctgtcacaac ctctttcttt atagaaataa
    20101 aaggacacta gcaattcatg aaaacgattg ctagagcgta tcatttgctt tagactatat
    20161 atcttggtgt acactcactt taaggcggaa ataaaagtta tccgtaataa caggtttagg
    20221 tatgaaaaag gagtgggtac tatgtacaag gaattagaaa acaaagtcgc agtagtaact
    20281 ggtggctcaa aggggattgg aacagcgatt gctcaacgat tcgggaaaga aaaaatgaat
    20341 gtagttgtca actataactc ggataaagaa ggagcagaaa aagcagtcaa agcaatcgaa
    20401 gaggcaggcg gaaaagcagt ctctgtacaa gcagatgtca gttctgaaga aggaatacag
    20461 cgtttacttg atacggcttt agaaaatttt gggacactgg acgtttgggt taataatgct
    20521 gggatggaaa atcagcatcc aacccatgaa cttagccttg aagattggga aagagtgtta
    20581 aaagtcaatt tgactggcgt ttttatcgga acgaaagcgg ctttaaatta ctttacagag
    20641 aataataaaa aaggaaatat catcaatatg tcttctgttc atgaacagat cccatggcca
    20701 acgtttgctc attacgctgc ttcaaaaggc ggggtaaaat tatttaccga aacggtagca
    20761 atggagtatg caccgaaggg aatcagaatc aatagtatag gccctggtgc tatccgtaca
    20821 cctattaatg cggcaaaatt tgatgatcct gaacaaaaag aactcttgga aagtatgatc
    20881 ccactaggac gcgtaggtga accaaaagaa atagcggcga cagctgcatg gttggcttca
    20941 gacgaatcca gttatgttac aggtattaca ctatttgcag atggtgggat gactttatat
    21001 ccttctttcc aaggtggaaa aggataaggg tgaataaaaa tgggtttatt gattgcattg
    21061 atcccggcag ttggctgggg gatccagcca ctgattttga aaaagattgg tggcagacca
    21121 acaaatgaga tattaggaac tggtattgga gcggtgcttg tcggcttact cgttcaatta
    21181 ttcgcttctc cagctggtat ttcagtgact acatttttga ttagtttact ttctggtgca
    21241 ttttgggtta ttggacaaac aggacagtat acatctttca acttgattgg tgtttctaaa
    21301 acgatgccaa tttcgactgc attacagtta gtcggaacct cgttgattgg tgtcttcgct
    21361 tttggagaat ggtctggaag tatggggaaa atcattggag cagcagcaat cattcttctt
    21421 gtatttggtt ctgctttaac ggcaatcagt gatgatcctg gaaaaaaagg cggtatcaca
    21481 aaaggaatta gtctattagc atcaacaagt attggttatt gggtatacag tgcattgcct
    21541 aaactagttg atgcaagcgg actttccatt ttctttccgc aaatgatcgg tgtcttttta
    21601 ggggcagtga tttatgtgat tttccgtaaa ccaaccgcat tttctgaaga caagagttgg
    21661 aaagcaacga tcgttggagc catctttagt gtgtcagcat tggcttatat tttttcagca
    21721 aatgaaaatg gggttgctac tgcatatatc attacacagc taaacgtagt tatctctaca
    21781 ttaggcggta tgtacatcct gcatgaaaat aagacaccaa aagaaaaaaa ttaacgatta
    21841 tcggattatt gctgatcgta gtaggaagca tggtcacggt ctttatttga ggataaagat
    21901 ggtaaaattt catttattaa aaaaatattt gtttgaacca aacagtaagt acttataaaa
    21961 aaatagcgct agaatttcca acatctctgg aatttctagc gctttttaat tgtttaagat
    22021 acaagggaaa tatctgtctg gatcaataat ttttcattga tccattgact aaagtttacg
    22081 acataattga agttaccacc gattagtatc tccgcatgat tgtctttata aagatcgtat
    22141 tgtaaaagac ggtctttttt atttgcgtaa acatcgttat ggtactggac taattgttct
    22201 cggaattgat tgattgctgt aaaatcacga tattgaaggt tgaaacgcat cgtgattggt
    22261 gcatttagca tgaatcgctg catttctttt gctgcttcct ctgcttgatc tccgtatagg
    22321 aactcaaaaa gattacgttc agaaaatttc cattgcccgc caacctttgt tccttttagt
    22381 tttccttcat gaagataatt tctgatagaa cgtgtagtaa cacctaattg ttcggctaat
    22441 tcatttactg agtaaaaatc tttcaatgtt caccctccta tgtgaatggt tctactgact
    22501 tagttgatat ttgcttaatc acgaaatgta gaaagaacga ttttaattaa ggtcattgta
    22561 aaaatcaagt cagaaaattc taacttttat ttctctaaag aaattcgttc tctcctaaag
    22621 tgatattata tttaaatata cctgaaaata atgggcagta ttatgaaaga tcactgatat
    22681 tttctcgtat tttttcctta ttcaaaagtt ttgcaggaaa attgctgaaa aaataatttt
    22741 actaagtaga aaattcaaag ccattcaatc agtgattttt ttcagcacaa gaaagactcc
    22801 ttataaaaat agcagatact ttgtcaatta agtgtttaac cagggatgat aaaaaggata
    22861 ggattgaaca aaacctgtat aaggtgatga atagtttcct cgccagtaaa aggtttattc
    22921 gtataaagct ttatcgccaa aatttatcat gacctttttt atcttcaata ttaaatgaaa
    22981 tcaattgttc aatcaattcc cagttgatta cacttttcca aggaactttg atgacgttat
    23041 ccgtatatac gtagcctgct tcttggatat ttttttcaag ggcacgaatc gctgcttttt
    23101 ccggtgcgat agaaaaatga tttttagcaa aactaaatgc aataataaag gtaccatggt
    23161 cagtaaacat aggttggttc cattttacag ttgtgtctag ctggggatac gtttctgtta
    23221 tcttagcaaa aaggtctttg atcttttgtt gatgttcttt ttctttgatt tgattgatga
    23281 tcggctgaaa tttttccatt ctaaaacgcc tccattaagt ggtttgattt attttaccag
    23341 aaaaacaata gttatgtata aaaaacgaac aaaacacaag taatgttgta accgctatat
    23401 ttatatgata agatgtctca aataataaaa tagaaaagag acataaaaat gattgacttg
    23461 gcaaagaaat tcttacacga aaatatatat tttgagttgt atcatcatcg agccatttat
    23521 acaaatgaag atgctttggt aataaagaaa gaacaaggat ttacaggtac agagaccaaa
    23581 agcttattct taaaagataa aaaagataat aactacgtat atctgacttt tactacgaaa
    23641 caaacggatt tcaaagcatt agggaagatc gtcggtaaaa ggctttctat tgtttcatca
    23701 gaacgcatgg aagaacaaac gggacaaaag cagggcgctg tttctccgtt tggttatgaa
    23761 aaacagattc cgattattat tgatgaagag ctcttatcaa atgaaaaact tgtttttgca
    23821 ccaggtagac cagatcaaac gatggtcatc caaacggttg acttaaacaa aatcatccag
    23881 ctacttgaga tgccgatatt tttgatgccg gcaaacgaaa aaaacaatta accaagtaaa
    23941 atagtgcata cttggaggtg aagcaacatg ataagttatc cattattaga aagaaatgca
    24001 acgattgggg ttactgcacc atcctcaggc gtatcagagg aacaccattc gttactcgaa
    24061 tcagctttaa agagaatgga agaaaaatct ttccaagtag ttactggaaa gacgctttgg
    24121 acgcaagata aggaaaaatc aggtccagca agaaaacgag cagatgaatt caatcaaatg
    24181 atgcaagatg aaaatatcca aatgatcttt ccgacttggg gaggagaatt attgattgag
    24241 attctcgatt ttgtcgattt tgatgctcta caaccaaaat ggatagtagg ttattcagat
    24301 attagtacgt tgctacttgc tgtcacatta aaaacaggga tagccactgc acatggaact
    24361 aaccttattg atttaagagg agagtacttg gatgaaacga cagcaatgtg ggaaagtgta
    24421 ctgaaaacaa gagaaggaga atcgattcta cagcaatctt ctgaaaagta tcaaaaagtg
    24481 tggccccatg agcattcatc atcttctgtt ttccatctca ctgaaccaac aaattggcaa
    24541 tcagtcagcg gagatgcagt aaggatgaag ggacgattat taggtggttg tattgatacg
    24601 atcaggcatt taatcggtac tccttatgga gatgtccaat cttttaaaag aaagtataat
    24661 gatggaaagc cgatcttgtg gtttctagaa aactgtgagc tatcgtcagt tgatttaaga
    24721 agatcacttg ttcagatgaa actggcaggc tggtttgatg gcagctcggg gatcatgttc
    24781 ggtagaagtg cggcagagga tttgttagat tataaaatga aggatgtata cgaagatctg
    24841 tcaaaggaat taaaactacc gatcttgtat gatattgatt gtggtcatgt accgccacaa
    24901 ctgacattga ttaatggtgc ctatgcagaa gtagcggtag aagatagaaa agcgactgtt
    24961 ttgcaaacgt ttatttaaaa aattttttcc tgccttggag tctatcaaag accaataaat
    25021 ctaaatagaa aagaaagtga aagaagctga caaaatgctg tcagcttctt tttgtataac
    25081 atattagaaa gtctaattat ctataactgt cacatcgatt aactgtttca cgtcattggg
    25141 atttcctgta ggaatagcta ctacagatgc attatcaatg atttcttgag ttgtttctct
    25201 aatgaacgct ggataagatg tttgatctgc aggcatttgc caatgtgaag gaactggata
    25261 actagtgatt gtcccgtcat ggttacttga atacactaaa actccctggt aactgtattt
    25321 cccagtgagc ataaccacat ctttcggata gactgcactc cctgcatcat aaggggcgat
    25381 aggtgttcct gcaggataat gaataacatg taattcgaaa tcacccgatg tatctctaaa
    25441 agtattacct ctaatagcta gccaaacacg tgcgtactca atttgtagat cagaatatcc
    25501 tgctaacgga tctattgact gagaagactg tgaggaggtt gtgtctattg ttgaagaaga
    25561 aatatttgtc tcaactgtac tactagatgt agaagaagta ttgggctctg agactttact
    25621 gcttgttgaa gatgtattcg tttcagtaga agctgatttc tgagtacttg atttttcagc
    25681 tgaactggtt tgtctgattg aacttttgtc tgtggtcgca gatggatcac tattttcttt
    25741 actaggagac gtacatccta caaggaataa agagaccaac aataaaataa aacctaactt
    25801 tttcatataa aatacctcat tattttttta ataccagtat agcataatcc tgaatttttg
    25861 aatccgtaaa accttgtcta tgaatataaa tagagtgtat cttctttgat aaatgtcaga
    25921 aaaagtgtga caaataatca tcagtaaaat tacttgtcac actattgata aggagtattt
    25981 aattgaagag aatgattagg agaaaggatc tccgtacttt ttatagtaat catagaatcc
    26041 aagtttgtat agacgaatcg ttttttctct tatttcttct gtttcaaaat ggactgcttg
    26101 aagaatcaag tctgtgaatt ctataggagc tgttccattc gcagtaatca aattacgatc
    26161 tttcacagct tgttgttcta taaaatcttc tttattctga taattttccg attctaacca
    26221 aggaaattgc gcatttccgg tgtgtttgta accttgcaat aatccgctct gagctaaata
    26281 atcaactgcc ccacaaatag cagctactgg tacgtctgtt tttaacaatt tatcgattat
    26341 ttgatataaa tgatgatttt ttatgtccca tgaattgcca ccgatcataa taaaaaggtc
    26401 aactttttca ggtatttgtt cgattaagta atcgattttt gtttttaatc ctccaatgga
    26461 agtgacctct gattggatag aagcagtttt gttttgccat ttctcatttt ggtttagtgt
    26521 gctagtaata taagcacctt cccaatcggc atattcctct aatagaaacg taatcgcagt
    26581 tttcattttc aagctctcct ttactatcta taacaaggac aacagtttta acctacagac
    26641 attcattaaa attgggtagc gacagcgaaa acaacaattc ttaagtttta gacagagtat
    26701 acagatagtc aacttaatta aatacgaact aaataaaaat gacaggtaat catttttgtt
    26761 taccagcatt ataacatttt gcatacatgg atgacaaaag aaccaattcc cacttgcttg
    26821 agtacaatat taaaagaaac tggaaggcaa aaagtaatta tagaaacgaa aaataaatca
    26881 tatcgggcac tcagacatga aaacaaccct agagatatat aactaggtaa ccagtttaat
    26941 gaaagaaaaa atcatccaag aagtcgattc ttggattttt tagtggattt gttcgccaaa
    27001 tacgtttagt atgcgtatat gagaggttgt aagcaaagcg ttgtggtctg agcacttaag
    27061 gaggaatctg tgaaaatagc ttttcatatt tttgcagatt ttgacttaat gccgaaggtc
    27121 agctttgcga ttaccgttta tcgagaggct gtaaaagcac cgtgtagctc cgatgacaat
    27181 aagcaagcat ttttagagct aggtttcctt gattctaaat atgcggctta tttcaatgag
    27241 ttggtgcttg aacgcgattt taagaaggtc gaaaacaggc acgacttttc gacgtaagga
    27301 gaaagaattt caatgataga aacaataaat cccgcagaaa acaaggtttc tgcgtacatt
    27361 catatcccat tttgcgagca tatctgctat tactgtgatt ttaataaagt ttttttagaa
    27421 gggcagccag tagatgagta tgtggaaatg ctgctgaatg aaatgaaaat aatgctagaa
    27481 agtcatccga cagcgaattt agaaacactt tacgtagggg gcgggacacc aacttcttta
    27541 tctgctaaac aacttgatcg actgttatca ggtgctagag agatcctgcc ttttcaaaat
    27601 ggtaatgagt ttactgtaga agcgaatcca ggtgatttga cccgtgaaaa acttcaagta
    27661 atgaaaaact atggcgtcaa tcgtttatcc atgggagtgc agacatttga taatcgctta
    27721 ttgaaaaaaa ttggacgtaa acacaccgca gaagatgtgt atcaaactat gcaattttta
    27781 gaagcagaaa attttaccaa tgtaagcatc gatttgattt atgcattacc gggacaaaca
    27841 ctagaaggat atctggatac tttggaaaaa gctttggctt tggatctgcc tcactattct
    27901 ttgtactcat tgatcctcga aaacaaaaca atgttcatga actgggtacg acaaggaaga
    27961 ctcgaactgc ctgatcagga aacggaaacc agaatgtttg aagagacgat tgaagcaatg
    28021 gaaaagcatg gacgtcacca atatgaaata agtaactttg ggctctccgg acatgaatct
    28081 caacataacc tcatgtactg gaacaatgac cactattttg ggtttggagc tggtgctagc
    28141 ggttatcttg gagatattcg ttaccgcaac aaaggaccaa tccaacacta tttacgccca
    28201 ttacgagcaa atgaacgccc ggtattggaa gaagaaaaat tgacacgaaa aaatcaaata
    28261 gaagaagaaa tgttcttagg actaagaaaa aaaacaggga ttactaaaaa acatttctat
    28321 gatcgctatc aaaaaacaat tgaatcggtg tacggtgatg tgctaaggga attagagcaa
    28381 gaagggttac ttgttaatga agaagaccgt gtttatttga caccaagagg gacctttttg
    28441 gggaacaatg tgtttgagcg ctttttgttt gaaaaatagt ttaaagaata tttgtttttt
    28501 atctgaattt aaataaaaaa agttcaaaaa tacaataaag gactattgaa acgttgatat
    28561 aactggattt acatcaatta tttagaatta gcactcttgt gtttagagtg ctaaaaaatg
    28621 gagtctttct cttgacagga gaagggcttc ttgctatatt tataagtgga ttagcactta
    28681 gaatatatga gtgctaatga gaggtgagga agatgttaac aaagcgacaa aacgacattt
    28741 tacgtctgat catccaacac tatacgagta gtggcgtgcc cgttggttcc aaaacattga
    28801 tgtctgaggg agtcaaggcc agctcagcaa cgattcgcaa tgatatgaaa gcattagaag
    28861 atgaaggatt gctgttgaag actcactctt cttctgggcg gattccatct gctttaggct
    28921 atcgttatta cgtggatcat ttgttaaaac ctgctcgcgt agcagaagat gatttgctgc
    28981 tgattcgtca gtctctaggc agagaatttc atgaaatcaa tgagattatt aagcaatctg
    29041 ctgagatatt atctgagctg acaagctata cgactttttc attgggacct gaaataaaag
    29101 accgccgact gactggcttt cgtatcgtac ccttgaataa tcgacagatt attgctatcg
    29161 tcgtaacaga tatggggaat gtagaaagcc aagtgttcac tcttcccgaa tatatgggaa
    29221 gtcaggaact agaaaaaatg gtacagattg taaacgaccg tctagtcgga gatcctctga
    29281 tgacggtgta ccataaactt cgtacggaga ttccgatgat tcttcataaa tatttccaaa
    29341 cgacagaagg gatgtctcag ttatttgaca ccgttcttgg tcatgttttt gaagataagg
    29401 tttatgtcag tggacaaatg aatctcttgg attatgagcc ccgtcaagat cttgatcagt
    29461 tcaaatcgat gttttctttt atgaaagact cagacggact tacacaaatg atcactccga
    29521 tggatagcaa tattcatatc aagattggtg ctgaattagg caatgattta ttgcagaata
    29581 tgagtatgat tcaagcaagt tacgagatag atggtcatgg aagaggtaca atagccttgt
    29641 tagggcctac tagtatgcct tattctcgga tgttcggtct agtcgatgtt tatcgaagag
    29701 aactagcagc taaacttgct gattactacc gttcgcttga cttgtcaaac tcgtaatgtg
    29761 aataacgaag ctatttgcag ctgatcgttc tctctttctt agtaagagca atcaaacagc
    29821 tgactgaata atgaaaggaa gactgtatcg tgaaagaaaa tcaagaagaa ctagataaag
    29881 aaatgacaga tgctcagcca gaaccggaaa tcgatgtcga agctgcagaa gattcgggta
    29941 tttcagaagc tgaagcggaa gaattcgaaa cagcgaaatt aaaagctgag ttagaagaaa
    30001 tggaagataa atacttgcgg gctcgtgctg aaatcgcaaa tatggcaaat cgaggaaaaa
    30061 atgagcgtga gcaattacaa aaataccgtt cacaagattt agccaaaaaa ttacttccat
    30121 caattgacaa cctggaacgt gcattagcca cagaagtgtc agatgatcaa ggggcaggtt
    30181 tgaaaaaagg cgtggaaatg gtcttggaaa gtttgcgtaa cgcactggaa gaagaaggaa
    30241 ttgaaaaaat ccctgctaaa ggagaagcct ttgatcctaa tcttcatcaa gcagtacaaa
    30301 ctgttccagc gactgaagat acaccagccg atacgattgt agaagtacta caagaaggct
    30361 acaaattaca tgatcgcgtg ttaagaccaa ccatggtcat tgttgcacaa taattgtgcg
    30421 gatataaaac ttacactttc aattaaagaa ggagacgtta attatgagta aaattatcgg
    30481 tattgactta ggtacgacta actctgcagt agcagtattg gaaggaaacg aagcaaaaat
    30541 catcgctaac ccagaaggaa accgtacaac accttctgtt gtatcattca aaaatggaga
    30601 aatccaagta ggggaagttg caaaacgtca agctgtgaca aacccaaata cgatttcatc
    30661 aatcaaacgc catatcggtg aagcaggtta caaagtagat gtagaaggta aatcatatac
    30721 accacaagaa atctcagcaa tgatccttca atatatcaaa ggattcgcag aagactattt
    30781 aggtgaaaaa gtcgaaaaag cagttatcac tgtgccagct tactttaatg atgcacaacg
    30841 tcaagcaaca aaagatgctg gtaagatcgc tggtctagaa gtagaacgta tcgtcaatga
    30901 accaactgca gctgctcttg cctatggttt agataaaaca gatcgcgacg aaaaaatctt
    30961 agtattcgac cttggtggcg gtacttttga cgtatctatc cttgaactag gagacggtgt
    31021 ctttgatgta ttgtcaacag ctggcgacaa ccatctaggt ggggacgact ttgataacaa
    31081 aatcatcgac tacatggtag cagaattcaa aaaagaaaac agcattgact tgtctcaaga
    31141 caaaatggct ttacaacgct tgaaagacgc tgctgaaaaa gcgaaaaaag atttatctgg
    31201 cgtatcaagc acacaaatca gcttgccatt tatcacagca ggagaatctg gaccattgca
    31261 tttggaaatg acattgacac gtgcaaaatt tgatgaatta acagctgact tagtagaacg
    31321 tacaaaagta ccagtacgtc aagcgctaaa agatgcaggt ttatcacaat cagaaatcga
    31381 cgaagttatt ttagtcggtg gatctactcg tatccctgca gttgtagaag cagtacgtaa
    31441 agaaacagga aaagaaccaa acaaatcagt taaccctgat gaagtagttg ctatgggtgc
    31501 tgctatccaa ggtggcgtga tcactggtga tgtgaaagat gttgttttac ttgacgttac
    31561 accattgtca ttaggtatcg aaacaatggg tggagtgttc actaaattga ttgaccgcaa
    31621 tacaacgatt ccaacaagta aatcacaagt attctcaact gcggcagata atcaaccagc
    31681 agtagacatc catgtattac aaggtgaacg cccaatggct gctgacaaca aaactctagg
    31741 aagattccaa ttgacagata ttccagcagc tcctcgtggt attccacaaa tcgaagtaac
    31801 attcgatatt gataaaaacg ggattgtaaa tgtatctgcg aaagatctag gtacacaaaa
    31861 agaacaaaaa atcacgatca aatcttcttc aggtctaaca gacgaagaaa tcgaacgtat
    31921 ggtaaaagat gcagaagcaa atgcagaagc agataaagct cgtaaagagg aagttgacct
    31981 acgtaatgat gtagatgcat tgctattctc tgtggacaaa acattgaaag aattagaagg
    32041 caaagtggac gaagaagaag tgaaaaaagc agaaacagct cgcgatgaat tgaaagccgc
    32101 tgttgaagca aacaacatcg aagaaatgaa aactaaacgc gatgcattga acgaaatcgt
    32161 tcaaaacttg actgttaaac tatacgaaca agcggcacaa caacaagctc aagaaaaccc
    32221 agaagcagct caaggcggag cagatgatgt cgttgatgct gattttgaag aagtcgatgg
    32281 cgacgataaa taatcagttc actgaatttc gagagaaggc caaagttgct actttggctt
    32341 tttcttttga aaaatagttt gaagtatcgg tttttgtttc aaacaaaata tgatatgata
    32401 acaagcgacg tgaaaatgat gagaaccaat aggaggaaag cctatggcaa caaaacgtga
    32461 ttattatgaa gtcttagggc tctcaaaagg agcatcagaa gatgaaataa agaaagctta
    32521 tcgtaagctt tcgaaaaaat accatcctga catcaataaa gaacctgatg cggaagaaaa
    32581 attcaaagaa gtatcagaag catatgagat tttaagtgac ccgcaaaaac gcgctgcata
    32641 tgaccaatac gggcatgcag gcacagatcc aaattacggt gctggtggag ctggtggcgg
    32701 atttggtgga ttcggcggct tctctggtgg cggcttcggt ggttttgaag atatcttcga
    32761 ctctttcttt ggaggaggcg gtcggtcagt cgatccaaat gcgcctcgtc aaggtgctga
    32821 tttgcaatat acggtcgact tgtcttttga agaagcgatt tttggtgtag aaaaagaaat
    32881 caagtataat cgggaagaaa tctgtcatac ttgtggtggg aacggtgcaa aaccaggcac
    32941 acaaccaact acttgtcata aatgtcatgg tgcaggtacg attaatgtag aacgccaaac
    33001 accacttgga cgagtgatga gccgtcaaac ttgtgatgtt tgtcatggga cagggaaaga
    33061 aatcaaagaa ccttgtccaa cctgtcatgg ctcaggacat gagaagaaag cacattcagt
    33121 taaagtgaat gtaccagctg gtgtagaaga aggacaacaa atgcgtttag ctggtcaagg
    33181 agaagctgga gaaaacggcg gaccatttgg tgatttgtat gtggtcttcc gtgtagaaga
    33241 aagtgatatc tttgatcgtg aaggttctga aatttactat gaattgcctt taagttttgt
    33301 acaagcagct ttgggggatg aagtccaagt accaactgtc catggagatg tgaaattgaa
    33361 gatccctgct ggaacacaga caggtacgaa cttccgttta cgtggaaaag gcgctccgaa
    33421 gttacgtggt ggtgcaacag gcgaccaaca tgtcaaagta aaactgatca caccgaaaaa
    33481 tctaaatgaa caacaaagag aagcattacg tgcattcgct gaagctggcg gcatcaaagt
    33541 tgaagaacag caagaagacg gtttcttcga taagtttaaa gatgcttttg gcgggaaaaa
    33601 gcggaagtag tttttctagt aaaaacgcta agtcattaat aattaaaaac cagacgaaca
    33661 atcatatgtt cgcctggttt ttttgtagtt gtcatgtttg taccaacaaa aaagccagct
    33721 ttagaatatg ataatcgtca tagtttctag gggtataacc agtgagagaa tggaacttgt
    33781 tcaatcgata ttgcaaggtg tttttatgaa taaataattc ttctgagcaa tttttgatgc
    33841 ttccgttatt tttactgaag catgccaaag tcttcttgaa caaatgtact tcttcttctg
    33901 tcaggctttt caaaatacgt tttacatata atgttcgttg atctgacggg attgctggta
    33961 aaacgatccc gaggtctaaa tcttcaaaaa agcggatctc gttcacagat tgatggaaag
    34021 ctatccattc gcaaacttgt ttggcatgaa ggaaagattg cggtgtctct tgctctgtat
    34081 aacaaagcga cccaattcca aagtttagct gtaactgata cattttatta aactttgtct
    34141 ggattttatc aagcaatgat agcaatgagg ctctggaaat ccgattgact aggataatga
    34201 tttgttgtct atgtacagtg agcaaagcct gtccgtaata agagaagacc ctttcgagca
    34261 actccaataa aggattgatc cgttcttgac acaattgctg attgtcagaa agttgtccca
    34321 caactacata atatggagaa gaaaaatcaa gctcaattaa gtgagggtgg tatcgactat
    34381 gtacttgata tcatcaatga accgatggta ctttcttatg cgatggaaca ggtagagcga
    34441 ttgcttgttt gtgcaggaaa acaagcgatg cggctgatac gattaggtga gaaaataaga
    34501 agataaaaat acacggtccc ttcatggcga ccgtgcattt ttggttttta ttaaatattt
    34561 aatactttat tcaagaaatc ttttgtccgt tcattttgcg gattttcaaa gatttgttga
    34621 ggcgttccat cttctaaaaa gtttccgtcg tcaatgaaca tgacgcgatt ggccacttct
    34681 ttagcgaacc ccatttcgtg cgtaacgata accatcgtca taccttgttc tgctaacttt
    34741 ttcatgacgt tcaagacatc accgaccatt tcaggatcga gtgcagaagt aggctcatca
    34801 aaaagcatga tgtcagggtt catcgctaga gcacgagcaa tagccacacg ctgtttttgt
    34861 ccaccagaaa gcatttcagg atacatatct tttttatctt ctagaccaac tgtctgcagt
    34921 aatttgattg cttttttctc tgcttcttct ctagattctt tcttcaggtc catcggtgct
    34981 aaaatgatgt tttccaaaat agaaagatga gggaataggt taaagtgttg gaataccatt
    35041 ccgatatgtt gtctcaccaa gttgatatct gtgcttttat ccatcaagtg ggcgccgtca
    35101 ataataattt caccgtcaga aggttcttct aaacgattca agcaacgcaa gaatgtactt
    35161 tttcctgaac ctgaagggcc aatgatacag acgacatctc cttcgttgat ggatacattg
    35221 atgtcgttta atactgtgtt atcgccgtat tttttgacta aatgctcaac taagattttt
    35281 tctgccatct tacttcacct tcttttctaa aacttttgct aattttgtca atgctgtgat
    35341 caaaatcaag tacatgatcg caatgatcaa gtaaacatac gtactttgtg ttgtacgtgc
    35401 aacaatgatc ttaccagttt gcagcaattc gactacaccg attgcggaaa tgatcgttgt
    35461 gtcttttaat gagataacga actgattgac gaatgatggg atcatgatgc gtatcgcttg
    35521 cggcaaaatg attttttgca ttgtccgatt ataagaaaga cctaaacttc tggaagcttc
    35581 catttgtccg acaggaacgg cgttgatccc gcctcgaaca atctctgcga tataagcact
    35641 tgcgttaaga gtcaaggtga taacaccagc aaggaagtca ggaatcgtaa atccaacgat
    35701 accaggcaac ccaaagaaaa taaagaacgc aagaaccatc attggaattc ctcggatgat
    35761 atcgacataa atactagcaa ttgtgcgaag agcgcgtatt ggtgctacac tgaataatcc
    35821 aaaaatcaca ccgattacta tagccaaagc aaaggagatc aaagttagga gaattgttct
    35881 ccaaagtcct ttcaacaatg cttgatagtt gttttggata agtccaagaa atgttgattc
    35941 atcagcagtg gaagactcac tggaagtttc tgaaccatcc gctacatatt ctttaataat
    36001 cttatcgtat tcgcctgttc tttttaattc tttcaatcct tcattgaaca tattaagaag
    36061 ctcaggattt tgtccttttt taacggcaaa tgcatatttt ccgccagatt cttttttgat
    36121 cggtgtttcc aattcttgtc cttgtttgat cgcataaccg attaccggat aatcatccat
    36181 cattgcatcg attgcgccaa cttttaaccc gtcgtacaat tgatcagcag catcaaaata
    36241 tttgatagaa tagccatatt cagattcatg gtcgcttaaa aattcagcac tttcggtacc
    36301 aacttttgct ccgactgttt ttcctttcaa atcttcataa gacttgatcg aagtatctcc
    36361 ttttttgaca gctaattgga tcccgctatc aaagtaagaa tcagaaaaat catagctttt
    36421 ctttcgttca tctgttacag tcattccggc aatcattccg tctgcttgtc ctgattccaa
    36481 agcttgcaaa gcagcagaga aaccacggta gtccattcgg atgttaaatc cttgcagttt
    36541 ggcgatgttt tccatcaatt ttacatcgat gccttcgtac tcgccctttg cgttttgaaa
    36601 ttcaaatgga gcaaaagcag agtcactggc aattacgtat acgtcttttt tgggctctat
    36661 tttttgcatt gtctcgcttg tgcttgactc tgccgttgta gtgttgtcag cttgtgcaac
    36721 atgaccaaac gcaagtgtca caagtcctgt cattaagaaa acaaatgagt gaaataaatg
    36781 ctttcttttc ataatcaacc tcctaaattt ccaatatttt attattatag gcgaaaagtg
    36841 ctgaatcaga caagactgta agctcgaaaa aatgaaaaaa atgcgagttt ctaacatttt
    36901 atgttataaa acataacatg tataataaga taaaaaagtg gcgaaagtat gttcggtgtg
    36961 gtataatttt ttagtaagga atgcctttgc tttccttttc tttttattgg tcgtaaaatg
    37021 aaaaggaatg aatgaaaagg aggacgaaga atgattgaga gagatacctc ccgacacttt
    37081 gagcttgtct cgaaatacca accggctggt gatcagcctg aagcgattaa ccaattagtg
    37141 gatggcgtag tcggcgggaa aaaagcacaa atcttattag gtgcgactgg aacagggaaa
    37201 acatatacga tttccaatct tattgagaaa gtgaataaac caacactgat tattgcccat
    37261 aacaaaacat tggctggtca gctgtacggg gaattcaagg aatttttccc taacaatgct
    37321 gttgaatatt ttgtttctta ttatgattat tatcagcctg aagcttatgt gccttcaagc
    37381 gatacgtata tcgaaaaaga ttcaagtgta aatgatgaga tcgacaaatt acgacattcg
    37441 gctactagtt cattattaga aagaaatgat gtgatcgtca ttgcatccgt ttcatgtatt
    37501 tttggattag gctcgccttt tgagtaccaa aaacaagtag tttcgattcg acagggagca
    37561 gaactagatc gcaatcagtt gattcgtgac ttagtcagta tccaattcga aagaaatgat
    37621 atagattttc agcgcggtcg ttttcgagtg cgcggtgatg tcgtggagat ttttccggct
    37681 tcaagagatg aacgtgcctt acgtgtggag ttttttggag atgaggtcga acgtatccga
    37741 gaagtcaatg cactgacagg tgaagtttta ggagaaacgg aacacgtagc aatcttcccg
    37801 gcgactcact ttgtgacgaa tgatgaacat atggagcatg ccgtagcgaa tatcaaagcg
    37861 gaattagaac agcgtttaac tgtcttgcga aatgaaaaca aacttctgga agctcaacgt
    37921 ttagaacagc gaaccaatta cgatattgaa atgatgttag aaatgggcta cacgtcggga
    37981 attgaaaatt attctcggca tatggacgga cgtaaagaag gtgaaccgcc ttatacgcta
    38041 ttggatttct ttcctgaaga ttttttgatc gtggcggacg aatcgcacgt aaccatgcct
    38101 caaatcagag gaatgtataa tggtgaccga gcgagaaaac agatgctagt agattacggt
    38161 ttccgtttgc catctgcttt agacaatcgg ccacttcggt tagaagagtt tgaaaaacat
    38221 gtcaatcaga tcatttatgt ctctgcgacg ccaggaccat atgaacatga acaaacagat
    38281 accgttatcc aacaaatcat ccgtccgaca gggctgcttg acccagtgat cgaagttcgt
    38341 ccaatcatgg gacaaatcga tgatctagta ggcgaaataa atgaacgagt cgaaaaagac
    38401 cagcgggtgt ttgtaacgac attgacgaaa aaaatggcag aagacctcac agattatttc
    38461 aaagaattag ggatcaaagt caaatatttg catagtgata tcaaaacgct ggaacgaaca
    38521 gaaatcattc gagatcttcg attaggtgaa tttgatgtgt tagttggaat caatttgctt
    38581 cgtgaaggat tagacgtacc tgaagtttcc cttgttgcta tcttggatgc ggataaagaa
    38641 ggctttttac gtagtgaacg ttccttagtc caaacgattg gacgagctgc tcggaatgaa
    38701 gaaggtaaag taatcatgta cgcagacaaa gtaacagatt ctatgcgtct agcgatggat
    38761 gaaacgtcaa gacgtcgtac gattcagcag aagtacaatg aagaacatgg gatcgttcca
    38821 aaaacgatca ttaaagaaat ccgagatttg atttctatta cgaaagaatc ggaagatgat
    38881 acaaaagaag cggtacaagt ctcgtacgaa gaaatgacga aggaagaaaa agacacgtta
    38941 ttgatgaaat tagaaaaaga aatgaaagat gcggcaaaag ctttggattt tgaaacagcc
    39001 gcgaatgtgc gagatatgat tttagaatta aaagcttcaa agtaggagga caacatggct
    39061 aacgataaaa ttacgattca tggtgcacgt gcccataact tgaaaaatat agatgtaacg
    39121 attccacgag ataaattcgt ggtcgttaca ggtttgtccg gttctggaaa aagttcccta
    39181 gcatttgata cgctgtacgc tgaagggcag cggagatacg tagagagcct ttctgcttat
    39241 gctcgtcagt ttttaggaca aatggataag ccggacgtag atagcatcga cggattgagt
    39301 ccggcgattt ctattgatca gaaaacaaca agtaaaaatc ctcggtcgac agtaggtact
    39361 gtgacggaaa tcaatgacta tttacgcttg ttgtttgcac gtgtaggaca tccgatttgt
    39421 cccaacgatc atatcgagat taccagtcaa tcagtagatc aaatggcaga tcaagtaatg
    39481 gctttgcctg agcgcacaaa gatccaagtg ttggcaccga tcgtggtaaa gaaaaaagga
    39541 caacataaaa aaatattcga acgaatccaa aaagagggat acgtccgcgt tcgagtcgat
    39601 ggtgagatct atgatttaag tgaagtgcct gagctggaga aaaacaaaaa acatgatatc
    39661 gcgatcgtca tcgaccggat cattgtgaaa gaagggattc gttctcgcct atttgattct
    39721 ttagaggcgg ccctgcgatt agcagacggc tatgctctgg ttgacgtgat cggtgaagaa
    39781 gaaatgttgt ttagcgaaca ctatgcctgt ccttattgcg gatttactgt tggcgaacta
    39841 gaaccaagac tgttctcatt caatgcgcct tttggagctt gtccagactg tgatggttta
    39901 ggtgtcaaat tagaagtcga tcaagattta gtgattccag atagaacgaa gacacttagt
    39961 caaggtgcta tcgttccatg gaatccaatc agctctcagt attatcctca aatgttagcg
    40021 caggcttgtg aaagtttcgg tatcgattta gatacaccat tcgaagaatt accggaagag
    40081 catcaggaga tcatcttgaa tgggacagat gatcttttcc atttccatta tgaaaatgat
    40141 tttggtggcg ttcgcgatgt agaaacgact ttcgaagggg tattgaagaa tatcgagcgt
    40201 cgttatcatg atacgaacag tgatttcacg cgtgagcaaa tgcgtcttta tatgacagaa
    40261 ttaacttgtc agacatgtaa aggttatcga ttgaatcccc aagctctatc tgttcaaatc
    40321 aatggaacga atatcgggca aacaaatgag ttgtctattc aaaaagcagt agatttcttc
    40381 tcaaacttaa cgttatctga acaagagaaa gtaattgcca aaccgatttt aaaagaagta
    40441 aatgacagac tttcattctt ggaaaatgtg ggactagact atctaacttt gagtcgcgct
    40501 tccggaacat tatctggggg agaagcgcag cgaatcagat tggctacaca aattggatcg
    40561 aatctttctg gagttttgta tattttagat gaaccatcga tcggattgca tcaacgcgac
    40621 aacgatcgac tgattggctc attaaagaaa atgcgtgatc ttggcaacac cctcgttgta
    40681 gtcgaacatg atgaagatac gatgtgggca gcagattatt tgattgacgt cggacctggg
    40741 gccggagaac aaggcggaca aatcgtggct gcaggcactc ctgaagaagt agctaatgat
    40801 gaaaattctt tgacagggaa atatctttca gggaaaaaat caatccctgt accaaaagaa
    40861 cgccgtaaag gtaatgggaa agcaatcaag atcactggtg cttcagaaaa caatctgaaa
    40921 aatattagtg ttgaatttcc attaggagaa tttgtagcag tcacaggtgt gtctggttct
    40981 ggaaagagta cattggtcaa tagtatctta aagaaatcgt tagcgcaaaa attaaataag
    41041 aattctgcta agccaggtaa attcaagaca atttccggct acgaaagtat cgaaaagatc
    41101 atcgatatcg atcaaagccc aatcggccgg acgccgagaa gtaatccagc gacttataca
    41161 agtgtatttg atgatatccg tgggttattt gctcaaacga acgaggcaaa aatgcggggt
    41221 tataagaaag ggcgttttag tttcaacgta aaaggcggtc gttgtgaagc ttgtcgcggg
    41281 gatggaatta ttaagatcga aatgcacttt ttgcctgatg tctatgttcc ttgtgaagta
    41341 tgtcatggta aacgatataa ttctgaaaca ttagaagtgc attacaaagg aaaaagcatt
    41401 gctgatattt tggaaatgac agtagaggat gctgtagaat tcttcaagca cattccaaag
    41461 attcatcgca aactgcaaac gattgttgat gttggcttag gttatgtgac gatggggcaa
    41521 ccagcaacga cattgtccgg tggtgaggca caacggatga aacttgccag tgaattgcac
    41581 aaaatctcta atggaaagaa tttctatata ctagatgaac caacgacagg acttcatagc
    41641 gatgacatcg cccgcttgtt gcatgtatta caaagattag tagatgctgg taacacagtt
    41701 ttagtgattg aacacaatct agatgtaatc aaaacagcag attatatcat tgatttagga
    41761 ccagaaggtg gagaaggtgg aggaacgatc cttgcgactg gaacaccaga agaaatcatt
    41821 aacgtaaaag aaagttatac aggtcactat ttgaaaaaaa taatggtata aagaggatag
    41881 atagctgaga tagttaaaaa aacataaaaa aatgaatttt tgcttgtttt attaatatca
    41941 gtgttctaga atcattacat ggtagttctt tctacactga ttggtataga taaaaacttc
    42001 ctataagttc aatccgttca aaaaagaatt gagcaatcgg aggactttcc tttgattgct
    42061 tttttcttta tggtatgata ttccttgtgt agttggaaaa gaaaaacaag agattatgag
    42121 taagtgagag ataatgatga acagtattga aaatgaagaa tttatcccta tattattagg
    42181 aagcgacatg aacgtttatg gtatggcacg ttcttttaat gaagcatacg gcaaaatctg
    42241 ccaagcatat gcttcggatc agttagcccc tacccgctac agtaaaattg tcaatgtaga
    42301 agttattcca ggatttgata aagaccctgt atttatcgag acgatgcttc gtttggcaaa
    42361 agaaagatat tctgataaga gtaaaaaata tcttttgatt gcttgtggtg atggatatgc
    42421 agaattgatc tcccaacata aacaagagct gtcagaatat tttatctgtc catatattga
    42481 ttattcttta tttgaaaggc tgatcaataa agtaagcttt tatgaagttt gtgaagaata
    42541 tgatcttccg tatcctaaaa cattgatcgt cagagaagaa atgttagtaa atggacattt
    42601 ggaacaggaa ttgccttttg aattcccagt tgctttgaaa cctgcaaaca gtgtggagta
    42661 tctttctgtc caatttgaag gaagaaaaaa agcttttatt ttagagacta gagaagagtt
    42721 cgatttaatt ttaggccgga tatacgaagc cggttataaa agtgaaatga ttgtacaaga
    42781 ttttatcccg ggtgatgata gtaatatgcg tgtgctaaat gcgtatgtag atgaagatca
    42841 tcaagttcgg atgatgtgcc ttggtcatcc actattagaa gatcctacac cagcatcgat
    42901 tggtaattac gttgtgatca tgccagatta taacgaaaaa atctatcaaa ccattaaggc
    42961 atttttggaa aaaatcgaat atacaggctt tgccaatttc gatatgaagt atgatcctag
    43021 agatggagaa tataaattat ttgaaatcaa tttgagacaa ggtcgtagca gtttctttgt
    43081 gacattgaac ggcttgaatc tggctcgttt tgtcacagaa gatcgagtgt tcaacaaacc
    43141 ctttgttgaa acgacctatg gaacgaatca atcagataaa gcaagattat ggatgggtgt
    43201 gcccaaaaaa atctttttag aatatgctcg ggaaaatgaa gacaaaaaac tagctgagca
    43261 aatgatcaaa gaaaatcgtt acggtactac tgtattttat gaaaaagacc gatcaatcaa
    43321 gcgctggttg ttgatgaaat atatgttcca taactatatt ccaagattca aaaagtattt
    43381 tcatgtgaaa gaaggctagt tcatggagaa ttttttcagt attttaggcg gaatgggcac
    43441 gatggcgaca gaaagttttg ttcgcttgat caaccaccga acgaaagcta caaaagatca
    43501 agaatatttg aattatgtct tattcaatca tgcaacagtt cctgatcgta cagcttatat
    43561 tttagatcga tcagaagaaa atccaatgcc gtttctgctg gatgatattg agaaacaaaa
    43621 tttattgcga ccaaacttca ttgtgttgac ttgtaacacc gcacactact tttttgaaga
    43681 gttacaagcg gcaacggata ttcctatctt gcatatgcca agagaagcag caaatgaact
    43741 tgtccgtcag catacgacag ggagagtagc gattctagga acagaaggaa gtatgaaagc
    43801 tggaatctat gaacgcgaag tcaaaaatct tggtttcgaa acagtgattc ctgatactgc
    43861 gcttcaagaa aaaatcaatt acctgatcta tcatgagatc aaggaatcgg attatttgaa
    43921 tcaagagctc tattacgaaa tcttagagga agcggtagag cgcctaaact gtgaaaaagt
    43981 catcctaggt tgcacagaat tatctttgat gcatgaattt gctgaagata atcattaccc
    44041 agtgatcgat gctcaatcta ttttagcgga tcgaacgatc gaacgtgcat tagctgaaag
    44101 aagcgaggca ttggatacag catcggaaaa gtaaaaaaat tggatttggg gcaaatatcc
    44161 taaaagatat ttggtccaaa tctttttgta aaagaaaaag aaaactggta atgatacgcc
    44221 cgggatgata taattttatt atgcacaatg tgagaggggt tcgtaaatat gacagataat
    44281 ttgcaattag tcatcataac aggtatgagt ggtgcaggga aaacagtagc tatccaaagt
    44341 tttgaagata tgggctattt ttgtatcgat aatatgccgc ctcgattgat tccaaaattc
    44401 tgggaattga tcaaagaatc aggtaaagtc acaaaaatgg ctttaggagt ggatttgcgt
    44461 tctcgttcct tctttgaaga aatccaaaac atgctgatcg aaatcgaaaa tacgaacttc
    44521 atcgacactt ctatcttatt tttagatgct tcagacgaag aactagtggc tcgctacaaa
    44581 gaaacaagac gagcacatcc aatggcgatg gacggtttga tcactgaagg gatacgtaaa
    44641 gaacgggcga tactagacga cttgaaagcc caagcttccg tgatcattga tacgacaaca
    44701 ttgacaccac gacaattacg tgaaaaaatc aatgagtcat tcaaatcaaa gggagaccaa
    44761 ggatttcgga tcgaatttgt atcatttggt tttaaatacg ggttgccgat cgattcggat
    44821 attgtgatgg atgtgcgttt tttgcccaac cctcattata tcccggaact aagaccattg
    44881 accggaaaag atcagccagt ctatgattat gtgatgtcct ttgatgaaac gcaatcattc
    44941 taccataaat ttttggatct tctggaaact gtattgcctg gatacattaa agaaggaaaa
    45001 agcagcttga cagttgcaat cggctgtaca ggcggtcaac accgttctgt ggcattgact
    45061 gaacgtttgg caaatgcttt agggaaaaac tataaagtca atgtgacaca tcgggacaag
    45121 gataaaagga aagagacggt gaaccggtcg tgaaaatgaa aacctaccgg ataagaaaac
    45181 caaagatcgt agtggtcggc ggtggcactg gtctgccagt tatcctgaaa agtttacgaa
    45241 atcaaggagc agatatcaca gcagtagtga ctgttgcaga tgatggtgga agcagcggtg
    45301 caatcagaga atcgatcgct atggctcctc ctggtgactt gagaaatgtc ttagtggcgt
    45361 tatcggatat gccacaattc tatgaagata ttttccaata ccgtttcaaa aaggaagatc
    45421 agtttttagc caatcatacg atcggtaatc tgatcattgc cgcagtttcc gagatgcggg
    45481 gaagcactta tgaggcgatt caattattag caaaaatgat gcatgtagat ggacatgttt
    45541 atccttcttc agaaactcct ttgaccttgc atgctgtttt taaagacgga acgactgcag
    45601 taggagaatc taaaatcgcg atcaaccgta aaaccattga ccgtgtgtat gtctgcaaca
    45661 caaatgataa aagtgaagcc aaagcggctc gaaaagtcgt ttcagcaatt atggacgccg
    45721 acatggtcgt tttgggaccc ggaagtttat tcaccagtat ccttccaaat ttagtgatac
    45781 cagaaatcgg cgaagcaatg ctcaagacaa ctgctgaaac cgtctatatc tgtaatatca
    45841 tgacccaaaa aggggaaacg gaacatttca cagatgccga tcatatacgt gttttacaca
    45901 agcatttgaa tcagcctttt atcgatacag tattggtcaa tacggaaaaa gtgcctgaag
    45961 attatatgga cccagagatc tatgatgagt atctagtaca agtcgcacac gacttcaacg
    46021 gattgagaaa tgaaggatgt cgagtgattt ccaccgactt tttagaattg aaagatggtg
    46081 gtgtcttcca cgatggagac aaagtagtag aagaattgtt ccgtttagtt ttcggttcaa
    46141 aatattaagc aatttattgt tggagaaagg aggagaatag gatgtcattt gctgcagatg
    46201 tgaagaaaga actaactggt ttggaagttc atcgagaaca tgcgaaagcg gaattagctg
    46261 ctttgctgcg aatgaacggc tctttaggta ttgtgaacca gcagtttatt ttgaacgtcc
    46321 aaacagaaaa tgctgcaatt gccagacgta tctattctct tttgaaagat cattatcagg
    46381 tacgaagtga gctactcgtt cgtaaaaaaa tgaaattaaa aaagaacaat gtctatatcg
    46441 tccggttgaa gcaaggcaca aaagatgtgt tgatggatct tgatatcatg gatggtatga
    46501 tgttcaatgg acacgtttcg gacgagatta tgggaaattc gcaaaaaatg cgttcttact
    46561 tacgtggtgc ttttatggcc tcaggttcag tcaataatcc ggaaacgagt cgttatcatt
    46621 tggaaatatt ttctatttat gaacagcata accaagacat ttgcgatatg ttaaattatt
    46681 acggtctaca tgctcgaacg cttgaacggc gcaatggcta tatttcgtat ttaaaaggag
    46741 cagaaaaaat tgctgatttc ttaacattga ttggtgcaac caactcaatg ctccgatttg
    46801 aagatgttcg aatcgtacgg gatatgagaa attcggtcaa tcgtctagtc aattgtgaga
    46861 cagcaaatct gaacaagacg attgatgcgg cttctaaaca aatcgaaaac atcgaattga
    46921 tcgaagcaaa ggtcggtcta catgcccttc ctgaaaaact tcaggaaatc gcagaacttc
    46981 ggcttcaaca tccagaggtc agcttgaaag aattagggga aatgattccc tcaggtgcaa
    47041 tatcaaaatc gggaattaat caccgaattc gcaaaataaa tgaatttgcg gaaaaactgc
    47101 gtgaagaagc agtttaatcc aaagaaaaaa ctgatacatg aagcaggaga tgcgaatgta
    47161 tcagtttttt ttaaaagcta tacttgcttt tcttccgtca aactataatt ccaactttac
    47221 tggagtaaca taatgttgaa aggcttctat tgttttttaa tacaatcgta taaaaagcgg
    47281 ggagacaact gaatgaagat tttagtttta tacataaact tttgcttaat atagaagtcc
    47341 tgatatagat aggatcaagt atttatctat aactggagat taattaaaaa ctggaaggaa
    47401 ctgctcgatc aaccttgaga actgtttttc ttacttctgg tcaagattct cgtggttggt
    47461 ggataaggat ggttgataag ttcattggat tagctagtcg aaaacaacca ggtgtaatta
    47521 atgtagaagt tttaacaagg ggatggagtg atttattcag tgataatcat aagttcataa
    47581 atagtaattg ggatgtatgg ttggtatacg ttatacctga acttgaacta tcaagatttg
    47641 gtttagaatt tgagaatcga tgtttataaa gggagggcaa aaaatgtgct tttttacaac
    47701 ttttactatc attatgattg cagtactttt tatttttcta gatatagcaa agcgaaatac
    47761 agcattttta ttatatcggg ttttacttcg agcaggtctc ataacattta tatcgatcgt
    47821 tggatttttt ctattcacag ttattgtatt tatttggaga actacagcac caccattgcc
    47881 agagattaca tatggagaat ttccgtttag tcttgaatat gaaatagatg aggaaagaat
    47941 tttgattgaa gatacggtga ttgcagaatt tataggaagt tttagaggga atgtgacaac
    48001 tcaacctaga cgaagttgga gtataaaact tttaaataat agtggagagt tgaaagcatt
    48061 tcattatgaa ttcttaataa tagaaagtga agatgtaaca atcactttaa gtagtggctc
    48121 tgctggatat tttatgaatg atggtggaag caagccacag ctcacagtcc gtgatcgtat
    48181 tgcaggtttt atgcattttt ctcactatga cttagaagaa gctcgtaaaa tacttgcaga
    48241 atatgggatc acattaatca attgggaata tgcgccaccg ataagaaatt ttttcaatga
    48301 ttattattag tcttataaaa aacagaatgt aaaatttatg tgcgcgagta tttagttttc
    48361 cattttttgt ttgatagata tatagcattg ttgaagcatg ggatagtggt gccaatttta
    48421 tcaatgaggt tggtcaggca gttaacataa ttggtgatgc tataaatcca tttaattgga
    48481 attggaggaa ttaggtgtgg aaacagtgag attatggata ttcatgaaaa attttcgttc
    48541 ggctttgaga tatgttattc tggaatcaaa atcaggttat tatctgattg acacagatca
    48601 ctatactttt tggggaggct acctttttcc gatttttaat tggataatcc cccataaggt
    48661 tatgaaaatt aacttgcctg cagaagaaat aaatagatta atactcaaca aagatttaca
    48721 aaggaaaaga gagaaagaac attctaatgt tgcttggata ggtccagcat ttgcagtagt
    48781 gacttcttca attgtcggtc ctatgctaga gagtatttat agtgattttt ctattggaat
    48841 acgttattta tttgttactt taagtatagc tgttcttttg tgcataaagg gttatgttag
    48901 caagaagaat aaagatattt taacactaat cggtgtaaac aatttatcta gtactaaaat
    48961 tatactgttt cctgaatcta taaaagaagg atcaaggttg ttgataattt ttataatgct
    49021 tatactgttt tctgttttat ttggggtgat gtttatag
//
LOCUS       DACEUZ010000006        47691 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.006, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000006 DACEUZ010000000
VERSION     DACEUZ010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 47691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47691
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.006"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            95..538
                     /locus_tag="IXG44_000293"
     CDS             95..538
                     /locus_tag="IXG44_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ1657036.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(544..1728)
                     /locus_tag="IXG44_000294"
     CDS             complement(544..1728)
                     /locus_tag="IXG44_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HAQ1657037.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            1996..2694
                     /locus_tag="IXG44_000295"
     CDS             1996..2694
                     /locus_tag="IXG44_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HAQ1657038.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            2701..3378
                     /gene="nth"
                     /locus_tag="IXG44_000296"
     CDS             2701..3378
                     /gene="nth"
                     /locus_tag="IXG44_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HAQ1657039.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(3504..4037)
                     /locus_tag="IXG44_000297"
     CDS             complement(3504..4037)
                     /locus_tag="IXG44_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1657040.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYI
                     GYFPNKNNNFYFYRNKL"
     gene            complement(4334..6715)
                     /locus_tag="IXG44_000298"
     CDS             complement(4334..6715)
                     /locus_tag="IXG44_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAQ1657041.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(6705..7352)
                     /gene="recU"
                     /locus_tag="IXG44_000299"
     CDS             complement(6705..7352)
                     /gene="recU"
                     /locus_tag="IXG44_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HAQ1657042.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            7416..7955
                     /locus_tag="IXG44_000300"
     CDS             7416..7955
                     /locus_tag="IXG44_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HAQ1657043.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            8045..8464
                     /gene="gpsB"
                     /locus_tag="IXG44_000301"
     CDS             8045..8464
                     /gene="gpsB"
                     /locus_tag="IXG44_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HAQ1657044.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            8486..8853
                     /gene="rnpB"
                     /locus_tag="IXG44_000302"
     ncRNA           8486..8853
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="IXG44_000302"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            9062..10228
                     /locus_tag="IXG44_000303"
     CDS             9062..10228
                     /locus_tag="IXG44_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HAQ1657045.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            10274..11770
                     /locus_tag="IXG44_000304"
     CDS             10274..11770
                     /locus_tag="IXG44_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HAQ1657046.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            11851..12534
                     /locus_tag="IXG44_000305"
     CDS             11851..12534
                     /locus_tag="IXG44_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ1657047.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            13179..14369
                     /locus_tag="IXG44_000306"
     CDS             13179..14369
                     /locus_tag="IXG44_000306"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HAQ1657048.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            14471..15424
                     /locus_tag="IXG44_000307"
     CDS             14471..15424
                     /locus_tag="IXG44_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HAQ1657049.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            15493..16842
                     /locus_tag="IXG44_000308"
     CDS             15493..16842
                     /locus_tag="IXG44_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1657050.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(17025..17348)
                     /locus_tag="IXG44_000309"
     CDS             complement(17025..17348)
                     /locus_tag="IXG44_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1657051.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            17477..18640
                     /locus_tag="IXG44_000310"
     CDS             17477..18640
                     /locus_tag="IXG44_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1657052.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            18710..20059
                     /locus_tag="IXG44_000311"
     CDS             18710..20059
                     /locus_tag="IXG44_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAQ1657053.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMVFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(20104..20535)
                     /locus_tag="IXG44_000312"
     CDS             complement(20104..20535)
                     /locus_tag="IXG44_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1657054.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            20923..23565
                     /gene="alaS"
                     /locus_tag="IXG44_000313"
     CDS             20923..23565
                     /gene="alaS"
                     /locus_tag="IXG44_000313"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HAQ1657055.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            23765..24361
                     /locus_tag="IXG44_000314"
     CDS             23765..24361
                     /locus_tag="IXG44_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ1657056.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            24483..25631
                     /locus_tag="IXG44_000315"
     CDS             24483..25631
                     /locus_tag="IXG44_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAQ1657057.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(25669..26082)
                     /locus_tag="IXG44_000316"
     CDS             complement(25669..26082)
                     /locus_tag="IXG44_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HAQ1657058.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            26161..26592
                     /locus_tag="IXG44_000317"
     CDS             26161..26592
                     /locus_tag="IXG44_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HAQ1657059.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(26691..26924)
                     /locus_tag="IXG44_000318"
     CDS             complement(26691..26924)
                     /locus_tag="IXG44_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HAQ1657060.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            27107..28777
                     /locus_tag="IXG44_000319"
     CDS             27107..28777
                     /locus_tag="IXG44_000319"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HAQ1657061.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            28924..29310
                     /locus_tag="IXG44_000320"
     CDS             28924..29310
                     /locus_tag="IXG44_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1657062.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            29297..29995
                     /locus_tag="IXG44_000321"
     CDS             29297..29995
                     /locus_tag="IXG44_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ1657063.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            30000..30464
                     /locus_tag="IXG44_000322"
     CDS             30000..30464
                     /locus_tag="IXG44_000322"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HAQ1657064.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            30461..31372
                     /locus_tag="IXG44_000323"
     CDS             30461..31372
                     /locus_tag="IXG44_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAQ1657065.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            31607..32146
                     /gene="pyrR"
                     /locus_tag="IXG44_000324"
     CDS             31607..32146
                     /gene="pyrR"
                     /locus_tag="IXG44_000324"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HAQ1657066.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            32160..33443
                     /locus_tag="IXG44_000325"
     CDS             32160..33443
                     /locus_tag="IXG44_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HAQ1657067.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            33456..34382
                     /locus_tag="IXG44_000326"
     CDS             33456..34382
                     /locus_tag="IXG44_000326"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HAQ1657068.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            34401..35681
                     /locus_tag="IXG44_000327"
     CDS             34401..35681
                     /locus_tag="IXG44_000327"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HAQ1657069.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            35686..36762
                     /gene="carA"
                     /locus_tag="IXG44_000328"
     CDS             35686..36762
                     /gene="carA"
                     /locus_tag="IXG44_000328"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HAQ1657070.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            36767..39952
                     /gene="carB"
                     /locus_tag="IXG44_000329"
     CDS             36767..39952
                     /gene="carB"
                     /locus_tag="IXG44_000329"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HAQ1657071.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERTGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            40006..40782
                     /locus_tag="IXG44_000330"
     CDS             40006..40782
                     /locus_tag="IXG44_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HAQ1657072.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQSSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            40779..41711
                     /locus_tag="IXG44_000331"
     CDS             40779..41711
                     /locus_tag="IXG44_000331"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HAQ1657073.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            41708..42424
                     /gene="pyrF"
                     /locus_tag="IXG44_000332"
     CDS             41708..42424
                     /gene="pyrF"
                     /locus_tag="IXG44_000332"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HAQ1657074.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            42431..43060
                     /locus_tag="IXG44_000333"
     CDS             42431..43060
                     /locus_tag="IXG44_000333"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HAQ1657075.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            43048..43362
                     /locus_tag="IXG44_000334"
     CDS             43048..43362
                     /locus_tag="IXG44_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1657076.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            43400..44041
                     /locus_tag="IXG44_000335"
     CDS             43400..44041
                     /locus_tag="IXG44_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ1657077.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            44072..44776
                     /locus_tag="IXG44_000336"
     CDS             44072..44776
                     /locus_tag="IXG44_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HAQ1657078.1"
                     /translation="MKKNMDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKG
                     EETKIEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSE
                     HIINGKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVN
                     PDVFFPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPI
                     QPLNGREIYYFEEFEQ"
     gene            44773..45675
                     /locus_tag="IXG44_000337"
     CDS             44773..45675
                     /locus_tag="IXG44_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ1657079.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(45833..47539)
                     /locus_tag="IXG44_000338"
     CDS             complement(45833..47539)
                     /locus_tag="IXG44_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin/fibrinogen-binding protein"
                     /protein_id="HAQ1657080.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
BASE COUNT        15522 a         8348 c        10268 g        13553 t
ORIGIN      
        1 ttggtttcag attgggtttt cctacaaaat atttttatct ttgttgaata ggaaacaaaa
       61 aaacaatata atgaaagaaa aagaaggtgt agaaatggat aaaagacaac tgctaataga
      121 agaatatctg gatttagcta atgagattat tcatcgtaat aaaccagaaa tggaaaaaga
      181 atttgcagga tttactctaa atgaactaga agtgattgaa catatcggga aaatccctga
      241 tcctaacgtg acaaaattag cgaatgctag ttacatgact agaggagcca tcagtaagct
      301 aacaaaaaaa ctgatagcta gagggatcat aaacacttat caaagcgaag agaataaaaa
      361 agaaatctat tttcaattga cgccaatcgg acaaaaaata aggaatagac acgagcaact
      421 ccatcaaaaa tgggtacagc gagatgctac tgtatttgaa aatatgtcaa aagaagaatt
      481 tgacacagta tttcgtttta tcggtcgata cagagaattt atacgaagta gcaaatgaag
      541 ctactatttt ttattgtagt gataataggc aaaaatcata agcaaaaatc cgaataccgt
      601 cggaagaaat ggtgctgcag cataaagctg tcctgcgatt agcgggccga tgacgcgcga
      661 tagagattgt atactttggg taccgcccat tacaagacct tgctggttat ctgagacaga
      721 attggatagt tggccattga atacaggagt aaaaatagaa tcaccgaaac cataacagat
      781 aatcccaaat aaaaacaaaa tcggcagtag aagaattcca gataatgtga tgaatagata
      841 ggcgaggatt tcactgaaca tccctattct tgtgatctgc tgttctgaaa attttttgag
      901 caatcgaggc ataatgaaca attgcgagac agtatccaag attccaatca aagagaagct
      961 taaaccaacc aacccagcct tccactgaaa ggcgtcaatc gaaaattgag aaaagataga
     1021 ttgaagagag ccagctgcca accaaacagc aaaacctgct gtcaataaag gaataacgga
     1081 tgaaatcttg aataattgtt gtaattggtg aaaaggtctt acatgggaga aacttaaatc
     1141 tactgaacgt ttttttattg gaaggctttc tagcataaat gtgtagccat aaacggcatt
     1201 taagaaagta aagagtgctc caataaatat aggaacagaa ttccccaact cagctaatag
     1261 accccctata ataggaccca gcgttgttcc tataccgact aaagcaccca tccatccaaa
     1321 aactttggtt ctttcattag gttctgtgat gtcggcaaag taagcatata aggtggagat
     1381 ttctcctgcc gtcaatcctt caatgatacg accaaggaac aacatccata tcgaatttcc
     1441 taaaccaaaa agcaaatatc cgatacttga tcctaataaa ctgataataa ggatcggatg
     1501 acgcccaaaa tgatcactta agctaccgag aatgggggca gataagaagg cagctagagc
     1561 ataaacagac atgagcaaag tgatatagaa agcttgctga tgtatggagt gagaataagg
     1621 cagcgttaaa aacggaagta caggactgac gatagtaaat cctaatccag tgaataagac
     1681 agagacgtat ccaaaaactt tagaatgttt atttgtgaaa ctgtacatgt aaaaactcct
     1741 ttatgcaata atgttaactt ggaaacaaaa atagtgtata gtaattttgt ttccttgtca
     1801 acattctaag gaacagcgaa atctacttaa tttatttatt ccgaaaagtg aagatacgca
     1861 tcaaaaacat gaacaaacaa aaatcaagta gacgacattg taaatctgac cgatcagtga
     1921 agtaaataga aaagaaacct gttcttgctt cccatgtttt ttttacgtat actgtgattg
     1981 agcggaggag aaatcatgtt gaatttagaa aattacttaa gcgctggaca aacagtcata
     2041 tctaatcttt tacttaaaaa ttatcatagg atcggtctac aatctgaaga atttttattt
     2101 gttctacagt tgcatatggc acagcttgaa ggagatactt tccctgattt gcagatgatt
     2161 gctatggata tgggaatcaa acaagatcag atttttcaaa tactggatcg tctcgtaaca
     2221 aatggattta tcaagataga aacgacgatt gtcaacggta aaaaagcaga tcgatataat
     2281 ctttacccca tctatgatct attaggtgag tatttaaaaa cacaagaaaa aaaatacgaa
     2341 gaaaaaacac aagaaaaaga aatccagtca gtgtatcaat tatttgaaca agaatttgga
     2401 cgcccgcttt cttcgatcga gttccagcgg atcggacaat ggctagaaga agaccattat
     2461 cagccggaaa ttttgaagct agcactgcgg gaagccgtat taaatcaggc ttatagtttt
     2521 aattacgttg accgcatttt gctatcgtgg gaaagaaaaa atctacgtac aaaacaacaa
     2581 gtggaagaag accaaaaacg aagaaaacag cagcttcttc aaagagaagt agaacagtca
     2641 gctcaacaag aagaattgcc gaaagtctca ctgaaaaatt ggttggagga gtagcggaat
     2701 atgttaagca aacaaaaaac aatggaagct ctagaaacga tgtatggaat gtttcccgaa
     2761 gcacatggag aactcaagca caataatcca tttgagttat tgattgcagt gatcttaagt
     2821 gcccaggcga cagatgtatc agtgaataaa gcaacgccag atttgtttgc ctcttttccg
     2881 acacccgatg cgttagcaga agcttcaatc gatgaaatca ttcttaaaat aaaaacgatt
     2941 ggtttgtatc gaaataaagc aaaaaacatc aaagcgtgtg cacagcagct gattgaacgt
     3001 ttcgatgggc aagtgccaac gtcaagagaa gaattgatgt ctcttcctgg tgtaggcaga
     3061 aagacagcaa atgtcgtatt gggggatgca ttcgggatac cagcaatcgc agtagatacc
     3121 cacgtggaac gtgtctcaaa acgcctgagg atctgcaaac tagatgctac ggtcatggaa
     3181 gtagaagaaa cactgatgcg gaaagtacca caagaattat gggtaaagac acatcataca
     3241 ctgatttttt ttggtagata tcattgcacg gcaagaaatc ccaagtgcga ggtatgtcct
     3301 ttgctatcaa tatgtcaaga tgggaaaaat cggatgcgtc taaaagaaaa ggcgctaaaa
     3361 aagaaaccac gcctgtagac gtggtttctt taattgcaaa taaatgtatg aattatcttg
     3421 ttattatgga gaaaagctgt ttcgtttatc ttttttgcat gaaaaagtaa atgtacatac
     3481 tcagtagcta ggatggtaac ttattacaat ttattgcgat aaaaataaaa gttgttattt
     3541 ttattgggaa aatacccgat gtagaaaata atgccttcca atatacctat aaaataagtc
     3601 cagtattcca aaggattata taaataagtg acgtatctat ctaaaggtgt aataaaaatg
     3661 ataagtgata ataaaaaaat accatttata actaaacata aactaatgga tttgggcggt
     3721 ttcaggctca tcagaaaaat atagattctg gtgaacaaca accataaaaa agtgagaatc
     3781 attgcatgag agcttctttc cataaatttt cctttaagag tagtgcttaa aagtacagaa
     3841 aaaataataa atgcaaggat aggagcaatt aaagtaaatt tatttaataa taaacaaaat
     3901 ttttcagctt ttttattctt ggaataacga ggtattgttt tcgataaaaa aagatggtgc
     3961 agacaaatta tgataattaa gcatactatc agataactca aaatgaagcc ttctttcaaa
     4021 aaattattca aaaccatata ttgtattttt tatttgataa taacaatggg attattaaga
     4081 agtggcacta aataaaagac tagtgtgtat ttagccgatt taattataaa caagtaaatg
     4141 gataaagtat atgtaatgat aaaagaaaaa tggattttct aatttttaag ctaaatgtta
     4201 gatcacacat gtatatgctg gatagtctta taaaaaatca tgtaatacag cttggtacat
     4261 gatttttatt tgtagctgtt ttttacatag acacaaaaaa gaaaccacgc ttgtagacgt
     4321 ggtttctttt ttactatctg tttccagaag ctgaggtgct gctggatggt gtatggtcat
     4381 ttgcagcatc cgctccgcta gttgctggct cactggaaga agcaggagga gcagatgaag
     4441 acggtgtcga gctttcttcg gatgaagaag aaggaaccgt tgttgaagac gctggctcgc
     4501 ttgtttctgc tgtcgtgctt tcttttgagg actcagcagt tgattcgctt tgactagtag
     4561 agctagagct ttgagtcgtt gtctctgttg ttgagcttcc aggagtctgg acatagctgg
     4621 aagatggaat agttgttgat ggtgtaattg cattcgatct agctgtatat tgatctttgt
     4681 agtacagttc accgcccaca cgaattaatc cgcttggcat ctcccagtct gtatttgtca
     4741 cgttagctga aacatactgc ataagctcgc gataaacatc agaggctacg tgtgacgatt
     4801 ctgaagtgac aggtgtcatt tttttattgt aacctgtcca cacggaaatc gaataatttg
     4861 gcgtatagcc cgcaaatagg atatctggat acaccccaga agaaatacca agttttgcat
     4921 actcgtcatc tgtgtaattg gatgttccgg tcttaccggc ttgatataac ccagcgattt
     4981 gcgcattcgt accagttcct tcagtgatcg tatcttttaa aatgtctgtg atcatataag
     5041 ctgtttctgg agacatggct gtttttccat ctggctcata agtttcttct gttccgtctt
     5101 ggaagacgat tttattaaca tattgaggtt tgtaataagt ccctccattg gcgaaagccg
     5161 cataggctgc tgccattttt aaggaagaag ctccgtattt tgtcccatct tgttcttccg
     5221 tattactaga gatcgcattc gattgatgga tcgtactgta ttcgatccca agatttttta
     5281 agaaagaagc cactttatct gaaccgactt cattgaataa tttgactgct ggaacattac
     5341 gggataagta aagtgcttgg cgtaaagtaa tggtgcccat atattgatta tcccagtttc
     5401 ctacaggagt cgaagtccct tcatagttat acggtgcgtc agtaatagtt tttccagtag
     5461 aatatttcaa atattcaaaa gctggtccgt aatctgtgac aggtttcatc gtagaaccaa
     5521 aatcacggga agtattcact gctaagttgt tgcctaaagt tacgtcttct gcgatgtgac
     5581 gtccgccaat ctgggctttt actttcccgg tattggtgtc gataagggtg gaggcaactt
     5641 gcatctcgtc gtctgggtaa gagacatact gatccgtgtt gacgatatca tatagtttct
     5701 tttgggcgtc cagatccaaa ttcgtataaa tctctaaacc gtctgtgtag acattcttat
     5761 ctgttttttc ttgtacttca ttgatgactt ctttgacata attatcgacg atcttagtat
     5821 tgtcatcgga ttgagttagt tcttgcagac catccgttac cggtacatta acagcttgat
     5881 catattctgt ttgagagatt ttttcgtttt gaagcatcgt atacaaaaca gtatctcgtc
     5941 ttttcttcgc ctgatctgga taaacataag gatcataggc ggaaggggct tgaggcatcc
     6001 cagcaagcaa tgctgtttgc ggtaaagaaa gttctgataa tttcttacca aagtacatct
     6061 cagaagctgt ctccatcccg tacaagccgt ttgacatata cactttattt acgtagtagg
     6121 tcaagatctc ttgtttcgat tttttttgtt ccaatcttac agccatccac gcttcttggg
     6181 ccttgcgttt cagagtctga tcctcggcac tggtggagaa gaaggaaagt ttgatcaatt
     6241 gctgtgtcaa agtacttccg ccttgcagtc cgccagaggt aaagttagag agggcagaac
     6301 cgatgatccg aatagggtca accccgatgt gtttataaaa acggcgatct tctaccgaca
     6361 cgatcgcatc ttctaatgtt ttcggtaatt cattcgcaga aattttttca cgcttttctg
     6421 cgcctaaatc ttcgaatagt tcgccatctt gtgtatatag tttggaagat acggtagcgt
     6481 ctaatttttt atccgtcaat tcgggcgcat cttttgcgta ataccagaaa agtcctacac
     6541 ctgctaaaaa gaggatacag aaaaatgaga gtattcctaa aaggatcttg ataaataatc
     6601 cttttttatg tgttccagag gactttccag aacctttgcc agaattcttc ttcacactct
     6661 ttttaggagt aggctgtttc tgtcgtctag agcttctagt ttgttcattt gccattcttg
     6721 ctttctcctt caatagataa tgttgataaa tagaagtcaa ccgcgtccaa ataaggaatc
     6781 cgcggagaaa taccagtttt gatctctatt cctagttctt cgatcaaaga taaaggtagt
     6841 gatttgcgtc ctttttcctt ttgggcgtcc cagtaagcaa tcaaatgaac actattcaaa
     6901 taaaaacagc gcttcaaaga cgaaaaccat aacaagacaa agcagacccc attttgttcg
     6961 aggcatgctt tcatatgttc gatttgatgg gcatggaagt ttttaaaggg aaaagatgtt
     7021 ttgttcttgg tttcttttgc ttcaaagtcc aagtaaaagc ctcgatagac gccattatag
     7081 tctgtagttg aggcttgggt aaagtaggct tccttgatca cagcagcact tctcttagga
     7141 tagtctactt tcacgatttg gataggggta ggctttttat ggataaccgc caacccgcgg
     7201 gaaagataat atttattact ttcattgatt gcttcttcaa agcgcatccc ccgattgccg
     7261 aattcagtga tctgatttct tttttcttga attttttcga cgggagaggc attcttataa
     7321 taaggttggc catttggata gcgcaatacc atctttaatc acactcctag gccacattat
     7381 agcaaaaaag cggatagaaa gaaaaggaat ctgagatgga taagtcaaaa gtaatctatg
     7441 tgtctgggta tcgaagtttc gaactcggtg tttttaaaga aaatgatcct aaaatccaag
     7501 ttgttaaaaa tgttttaaaa aaagaactcc atcagttagc agaagaaggg ctggaatggg
     7561 tacttgtcag tgggaatttg ggcgtcgaac tatgggctgt gcaaatagta gcggaattga
     7621 aagaagaata tccggaacta tctcttggta ttttgtatcc tttttctgat tttggtaata
     7681 actggaatga agggaaccaa gaaaaaaaac aattagcaga acaattagca gattatgtcg
     7741 aagctgttag ccatcaaccc taccaatcac ctagtcagct aaagaatcac actcgtttta
     7801 tacttgagca tacagtgggc tgtctgctga tctatgatga agaattccct ggaaaaacga
     7861 agtatttttt tgaagatgca cagaattacc aagcagatca tccctacgaa attcggctga
     7921 tcagtatgga cgatcttcaa aattttagtg aataatcgat attgtgtttg atttatcaac
     7981 ttttttccta tacaatgaaa aaggtagaaa tgttattacg taaatgaaaa ctagaggtgt
     8041 aaatatggcg aatttggttt atagtcctaa agacatttta caacaagaat tcaaaacaaa
     8101 aatgcgcggc tatgatcccg ttgaagttga tgaattctta gacaatatca tcaaagatta
     8161 tgaaacttac agcaaagaac tccttgcttt acaagaagaa aacgatcgat tgagtgcaaa
     8221 agtagctcaa ttatctaaaa ctcaaggagc agctcaaaca cgcgtgcaac aaacagaggc
     8281 accaaagagc gcagcagtga ccaattttga tatcttaaaa cgcttgtcaa atcttgaacg
     8341 tgaagtattt ggtaaaaagc ttgatcaaca agcaagtgca gtcaaaccag cacaacctaa
     8401 tccaaataac tacacaaatg cagacacaag cttagatgat aatgaaaaaa cacgtcaatt
     8461 ttaatttttg attaatgaat ttgcggtttt cgggtaatcg cggtctgact tgatcaggct
     8521 gaggaaagtc catgctcgca caggctgtga tgcctgtagt gttcgtgctt agcgaaacca
     8581 taagctaagg tactctttgg agtaacggca ggaaaacaag ctaaggcttc ggctatgctt
     8641 aagtatcctt gaaagtgcca cagtgacgaa gcggctgggg aaacccatcc ggtggaacgc
     8701 ggtaaacccc tcgagcgagc aacccaaaca atggtagggg cgctttttgt ctggaagaag
     8761 aacaggacaa aaaggcagta taactgcaga tagatggtta ccgctttgtt ttttctccct
     8821 ggaaaagcaa agtacagaac atggcttata gaaaaccgca aacatcaggg aattaggtcg
     8881 tgacaaaagt aatgcgttat aagaaacaag aaaaacattt gaaagctatt tttcaatact
     8941 tcagatgaat cgttcttatt acaaaacaaa cttttgtatc accggttatt cggaatttga
     9001 gtaaaagtgt ggggtaggac tgatgtccca cctccttttt ttataaataa ggagattgtg
     9061 tatggaaaaa aacaggaagt tcaacctcgt cgcaactgcg gcaagcgggc tagaagcact
     9121 ggttggtaaa gaattaagag atcttggaat cgaatgccaa gttgagaacg gacgtgcacg
     9181 ttttgaagga acaatggaaa cgatcgctac agctaattta tggttacgta cagcagatcg
     9241 agtaaaaatc gtggttgggg aattcgatgc gtatacgttt gatgaattgt ttgaaaacgt
     9301 aaaagcattg ccttgggaag attttcttcc actagatgca gcttttcctg tagctggaaa
     9361 atcgatcaag tcgaaactat atagcacacc tgattgccaa gcgatcacaa aaaaagcaat
     9421 cgttgaacgt ctccgtacct attatcaccg tccagcttct gtaccactga cggaaactgg
     9481 cgcacatttt cagttggaag tagctttgct gaaagaccat gtgatggtaa ctctggatac
     9541 gacaggacca agtttattca aacgtggtta tcgcttagaa aaaggtggag caccattgaa
     9601 agaaaacatg gcagcagcac ttattatgtt gacaaattgg cgaaaagacc gtccatttta
     9661 tgatcctgta tgtggttcag gtacgctttg tattgaagca gccttaatag gccataatat
     9721 cgcaccagga ttcaatcgtg agtttgcttg cgaatcttgg gattggttca gtcaagaaat
     9781 catggaaaat gttcgatcag cagccgaaga aaaagcagat tatgatattg aattagatat
     9841 tactggttca gatatcaatg gacggatgat tgaaattgcc aaagccaatg cagaagaaat
     9901 cggattaggc tcttcgatta cctttaagca acaagcagtc aaagacttca aaacggataa
     9961 agaatacggg gtcatcgtgg ctaatccgcc ttacggagaa cgtctaggag aagaagaaac
    10021 tgttcggaaa ttatataaag aaatgggcga tgtattccgt ccattaaaaa catggagtaa
    10081 atatatcttg actagcgact tagcgtttga ggaattttac ggacaaaaag caaccaaaaa
    10141 aagaaaattg tacaatggtg cattgcgtac ggatctattc cagtattggg gaactcgtcc
    10201 gcctagaaaa aaagagaaag acgaataatc ttactaattg attcattcaa ttggatgttt
    10261 ggaggaatag ggaatgaaag aaaaagagtt tttacaagaa gtaaaagaaa tcaatcttct
    10321 acaacaagct ttaggaatcc tcgattggga tacacaaaca ggtatgcctg aaaaagctag
    10381 tgcataccga agtgaagtag acagctatct ttacagcatt tatttttcaa aaaaaatcgg
    10441 tccaaaaatc aaagaagcca ttcgctattt ttctgaacat ccggaagaat tgtcggaagt
    10501 tggaactgcc gtttttgcca aagtaaaaga agagtatgat ttggaacatc gcgtaccgga
    10561 agatcttatg agaaaatata cagctgccgt ttcttcagca cacggacaat ggcaaaaagc
    10621 tagagaaaca caacaatttt ctgattttca acacgcattg aatcaaaata ttgaattaac
    10681 aaaacagttg ataccttact ggagaaaaga tgaaaagacg aattatgatg ttttgctgaa
    10741 tcaatacgaa cccgggatga ctgtcgaaat ccttgataag gtatttgatc agcttaaaga
    10801 aggaattctt tcgatccgcc gtacgctcca agaaaaaggg actgaacctg aaacagactt
    10861 tttaaatcgg agaatgacga aaaaacaaca gaagagattt gtagtgaaag taatcgaaca
    10921 acttggttat gattttagtc gtggtcgttt agatgacact gtccacccat ttatgactgg
    10981 aattaatccg aatgatgcac gaatcacgac acgatggaac gaaacagatt tcaaaatggc
    11041 tgtttttgga gtgatccacg aggctggtca tggtatgtat gaacaagaca tcgatgataa
    11101 atatgcgtat acaccggttt atgaaggtac atcaatgggt atccatgaat cacaatcgtt
    11161 gtttaacgag atcattattg gaagtagtcg cagcttctgg aaaaagcaat atcctttctt
    11221 tcaagaatgt gcagaaggaa cattcgatga tattcctttt gacaaatttt atcgttcatt
    11281 gaaccataca aaatcaagtt tggttcgtat cgaggcagac agtttgactt accctctcca
    11341 tatcatcatt cgttatgaaa tcgaaaaaat gattttcaat gaaggggtag atgttgctga
    11401 actacctgaa atatggaatc aaaaatatga agagtatctg ggaatacgac caacaaatga
    11461 tgtggaaggc atcctacaag acgttcattg gtcaggtgca agctttggtt attttccctc
    11521 ttatgcatta gggtacatgt atgcggcaca attacttcat tctttgcagc aagaaataga
    11581 tgtggaaaat gttctagcgt cagacgatta ttctccaatc aaaaactgga tgtcagagca
    11641 tatccatcaa tatggtgcat ctagaaaacc aaatcaatta atcatggatg caacacatga
    11701 accgcttaat ccgcaatacc tgatcgacta tatgaaagac ctgtatttta atgtatatca
    11761 ggtaaaataa aatacaattt acaaagatag ctgaaattca tctaatctat agatgttttc
    11821 agctatttac ttaagaaaga agtggaacga atggataaat tacaagtgat cgctgattat
    11881 agtatggacc aattagcgca tgatcaaaca ggacatggga gcgatcatac caaaagagtt
    11941 gtcaagttag ctgaaaaaat tttggagacg gaaccgcagg cagaccgctt tgtgacgctt
    12001 gcagcagctt acctacatga tacgattgat gataaagtcg ttaaaaatga gaacgaggca
    12061 aagcaacagc ttcgtgtatt ccttcgctct ttgccaatag tagaggaaca gatctcgatg
    12121 attttttcca ttatcgaaaa tatgtccttt tctaaaaatc tctctgaagc ggccgaacta
    12181 tctctggaag gtaagatcgt tcaagacgct gatcgtatcg aagcattagg agcaatcggc
    12241 attttgcgta ctgcttattt cggcggtgga catgggcacc cgatttttga tcctgaactg
    12301 tatccgcaaa cattcaagga caaagaaaat taccgaaaag gcacgacagt gatcaatcat
    12361 ttttatgaaa aactgttctt gctgtctgat aaaatgaata ctgattacgg atatgaagaa
    12421 gccaaacgaa gagaaacctt catgcgtgaa ttcttagaag aattttttct ggaatggtct
    12481 attgacgatg aaaattttac tccagacaat tggatcaaaa ataacaaaaa atgatggagg
    12541 ttgtgacaga agtggtcagc tccaagatat ttcggatcat tttccgtaaa agttacttct
    12601 ggaacactgt ttaccaagag gtcgtgagcc tgccgttcag ctccgagtca caaggaacaa
    12661 tttcaaaaaa tagcttctcg tgtttgatga atattgtaac ttgtgattcg gaattggcag
    12721 gagaacaccg tttattcgga agaataggac tgtgacaaga cttttgtcac agtccttttt
    12781 tcgaattcaa caagtaaaac ttttaagaaa tattcagaga gtaaaattaa aaattgctga
    12841 taaacgaaga tggaaatttt atgtattttt attggtctat gcaataaata aattaaatat
    12901 tcagaataca attgatttta acgaaagggc ttctatcaat ggttgtgttg tatttatctg
    12961 cccaggtaga gtaaaataga tcattatcta aaaggtaatt gttcatacta tataagtcat
    13021 ttgaaagtaa aaagatactg taaataaaaa atgtggttaa ataattctca taatcagtgt
    13081 aagcgttatc aaaaatatag catacttgct ttgtagatga atgacacgat atatgtttgg
    13141 tatgttctgc aagacagatg aatatggagg tggagacaat gtcttccaaa gaactaacaa
    13201 tgtatttaga gaaagaattg gatcaactga aagaaaaggg gttatacaat acgatcgaca
    13261 tactagaaag tgaaaatggt gcttgtatca tcgtagacgg aaaaaagatg atcaatctag
    13321 catccaacaa ttatcttgga tttgccaatc gcgaagaatt gaaaaaagca tgtattgaag
    13381 caaccgaaac ttatggtgta ggagcaggag cagtccgaac gatcaatggt tcgctgaaaa
    13441 tccatcagca attagaagaa aaaatcgcag aattcaaagg gacagaagca gcaatcgcct
    13501 ttcaatctgg attcaactgc aatatgggcg ctatctctgc agtaatgaca aaagaagatg
    13561 ctatcttatc ggatgaatta aaccatgcat ctattattga cggttgccgc ttatccggag
    13621 caaaaatcat ccgaattaaa catcaagata tgaaagacct tgagaaaaaa gcaaaggaag
    13681 cgatagaaag taagaaatat aaaaaaatca tgtatatcac agatggtgtt ttctccatgg
    13741 atggagacat tgcacgattg ccggaaatta ttccgattgt tgaaaaatat ggactgatca
    13801 cctacgtcga tgatgcgcac ggctcaggcg taactggaaa aggtgcagga accgtcaagc
    13861 attttggatt aagcgacaaa atcgatatgc aaatgggcac gttatcgaaa gctataggtg
    13921 ttgttggggg ttatgtagct ggatcgaaaa cactaatcga ctggttgaaa gctagaagtc
    13981 ggccattcct attttctact tcgctaactc caggtgcagc tgcagctgca ttggcttcga
    14041 ttactttgat gcaagaacat ccggaacttg tcgaaaaagt ttgggaaaat gctaattatt
    14101 ttaaagaaga attgaaaaaa gtaggctata acatcggtat gtctgaaaca cctattactc
    14161 cagtcatttt gggagacgaa aaagtgactc agactttctc taagaagctg atcgaacatg
    14221 ggatttatgc taaaccaatc gtttatccaa ctgtgcctct tgggacaggc aggatcagaa
    14281 atatgccgac tgcggaacat acgagggaaa tgcttgatga agcagtagca gtttatcaaa
    14341 aaataggaaa agaaatggaa attatttaac taaatttagt tttttcgtct atttaaaatt
    14401 agagaacatg ttcacgcata tattgttaat catctgaaga tagtagtaaa aaagatcagg
    14461 gaggatcatc atgaaaaaag tattggtgac aggatgttta ggacaaattg gttcagagtt
    14521 gatcacacgg ctacgtcaag aactaggcag tgaaaatgtc attgcgacag atatacgcat
    14581 gcctgaagtc attgagaaaa acgatatctt tgagatccta gatgtaatgg attacgaaaa
    14641 aatgcgcagc atagcagaac aatatcaagt ggacacattg attcatttgg ctgctctttt
    14701 gtcagctgtt gcagaagcaa aaccacaatt agcctggaat ttgaacgtga ctgggctggt
    14761 caatgcattg gaagtcgcta gagaattcca gctgaaattt tttacgccaa gttcaatcgg
    14821 cgcgtttggt ccaaatactc caaaagatca taccccacaa gatactcttc agcgtcctac
    14881 aaccatgtat ggggtaacga aggtagctgg agaactacta tgtgattatt attatgtgaa
    14941 atatggcgta gatactcgtg gtgtccgttt ccctggattg atttcttatg caaccttgcc
    15001 aggaggcggt acgacagact acgcagtcga tatttattat tcagcaatca aagaaggaaa
    15061 gtacactagt ttcattgatc aaggtacatc aatggatatg atgtatatgc cagatgcgat
    15121 cgatgccatt ataaaactca tgaatgcaga tcctgatcga ttggttcatc gaaatgcctt
    15181 caatatatcg gcgatgtctt ttgacccgga agagatcaaa gcatctattc aaaaaatcat
    15241 accagatttt gaaatggatt atgatgtgga tcctgtacgt cagtcaatag cagaatcttg
    15301 gccaaattca ctagatgttt cttgtgcgca agaggaatgg ggcttttcgc cgaaatacga
    15361 cttagatgtg atgacaaagg atatgttgga aaaactgaaa gaaaaattgg tagagacggt
    15421 ataaaacaag aaccacatcc aaaaagtttt ttatactttt gggattggct atggtatgaa
    15481 aggggacaag ggatggaaga atatcaagcc acgccagtaa aagaagtaaa aatagaaaat
    15541 gaattaaaac gtacgatggg attttttact gctttatcga ctgttatggg cacagtgatc
    15601 ggtgcagggg tattttttaa agccgcaagc gtagcagaag ttacaggatc cgccagtttg
    15661 catatgtttt cttggtttct cggtggtatg atttctgtct gtgcaggact aacaggagct
    15721 gaactggcag ctgctattcc agaaacagga gggatgatta aatatatcga acggatttac
    15781 ggaaatacag cagctttttt attgggctgg gcgcaagtcg tgatttattt tccagcaaat
    15841 gtagcagcct tgtcgatcat tttcggtaca cagttcgtca atctatttgg tctttcccag
    15901 tcaatgattg ttcccgtagc tgttacagct gctgtatcga tcctgttgat caatttttta
    15961 ggttctaaag ctggaggggc ttttcagtcg attacgcttg tctgcaagct tattcctttg
    16021 tttgtgatcg tcattttcgg tttgttccga caagaaggag tagacttcca actgtttccg
    16081 atccaagcag gagaaaatct ttcatttttc tcagccttgg gagcgggctt gctagcaacg
    16141 atgttcgcat acgacggttg gatacacgta ggaaatattt cgggagaatt gaaaaagccg
    16201 gcgaaagatt tgccaaaagc aatttcttta ggaatcatcg gaatcatgat cgtttattta
    16261 ttagtaaatg cggtgttctt aagaaccgca tctatcgacg gagtcgcagg aaacagcaat
    16321 gcggcaagtg acgttgcaaa aatgattttt ggcggcttcg gtggaagact ggtcacagtt
    16381 ggtatcttga tttctgttta tggaaccatc aatggctata cccttactgg aatgcgtctc
    16441 ccttatgtta tggcaaaaga aaacaacctg ccttttagca agctctttgc taaacttcat
    16501 gataaaacta aagttcctgt agccgcaggt atcttagagc tagtcatcgc tattggcatg
    16561 atgatgatcg gtggttttga tacactaacg gatatgctga tctttgtcat ttggattttt
    16621 tatacgatgg tctttgttgg tgtcattctt cttcgtaaaa aagaacctga tttgtttcga
    16681 ccatacaaag ttccaatgta tccgtttatt ccactagtag caatcattgg agggactttt
    16741 atcgtatcaa gcacgcttat cactcagacc tttttagctt cgatggggat agtattgaca
    16801 cttgctggta tcccgattta tctctattta aaacgtagat agctaaaaca ttcgaatttt
    16861 attagaactg cacttctctg ccaaacaaaa tattttattt aaatgaggag tacgtggaag
    16921 aaatgtcaaa agtactcgtt aattttgatc acgtaaatgt cgttttgtct acgatgaaat
    16981 agcaaagtaa aagacagaaa tcgggctcaa ccgatttctg tcttttactt tgatttgcgt
    17041 aactgaacta ttcggcgtac gccgcgataa atatttaata aaaccaaaca accagaaatg
    17101 aaaatgggaa gccaaatcag atagacaggg tagccaagca ctttagacaa agcatgtgga
    17161 gtcattttat ccatttcaag aagtgcttgc tgccaagtct gaagattgaa gactagcatg
    17221 aaacccgaaa taatcaggag aataaatcct aaataatgaa ttgtagttct ttttcttaca
    17281 taagcgatcg tagcataaag gaacagtaaa aaaataggta cgccaaaaac aaagtacata
    17341 ttcagcatct cgacaccccc tttgtgttta tcttacttta ctttgaaaga aatgagaaat
    17401 aatagcctcg ttacttgctt gaaacagcaa aggactgcta agataagaga gatacaaaga
    17461 atcaaggagg agtcatatgg caagttatat cgaaccgatt cgttttgcgc tgattgtttt
    17521 tccattttta gccttagcga tctcatctgt tttttttatt tatgaatatc gaaagtatgg
    17581 aacatttcta gtaacaaggg cagttatttt gtattctttc gttttttatt tattgtgtgc
    17641 gtattttttg gtgatcttac cacttccggc aaaggagacg gttgcccaaa tgacagggcc
    17701 aaagatggag ctgcatttat ttgcttcatg gcaacatttc agagacaaca cggtattggt
    17761 cttgtctgaa cctagtacct acctgccggc aatgaagcag agtgtatttt tggaaccagc
    17821 ttttaatgtt ttattactca taccgtttgg catttattta agatattatt tccggctttc
    17881 ttttatgaaa acgatgattg ctagtttctg cctttctcta ttttttgaat tgacgcagct
    17941 aagtggactt tattttatct acccaagacc ataccgctta tttgatgtga atgatttagt
    18001 gacaaataca ttgggtggaa tcattggttt tgtcgttacg ccgttattta catttatgct
    18061 tcctacaaga agacgaatgg atgaactttc ttatgaaaaa ggaaagactg tctcattgac
    18121 tcgacgcttg gtcgcttatt tgatcgactg gacattttta tctcttatta atttggtagc
    18181 ggcgattttc tttaaaattg cgttcgattc aggggcagtt acttctcata tctggtggct
    18241 tttattagaa gtaggggtat actttatgat tgtaccgttt ataaccaatg gatggacact
    18301 tggcaaaaaa attgtgcgta tccgtttggt ggaagaagac cgcgaacgaa tttctttaaa
    18361 agcattgatg atccgctacg ggtatctgta ctttcttttt tatggtttaa gctggttggg
    18421 gcagttgctt cagcaaatca tgaattcgac aacgaatacg gtgttgcaga tgagtgcagt
    18481 attcatttct ctcggtgtct tgttactaca aggtattttt attttaaata tcttgctatc
    18541 atttttgaga aaaaaacgga ttctgtttta tgaaaaagct agtcacacat atactgttag
    18601 cacgattggc caaacaaagg atgatgattc tcgaaattag ctgagcaaaa acgctttgtc
    18661 tgtgttataa tgccaaagtc gtgataattt aaagagaaga ggtgcacgaa tgaataattt
    18721 tgatatattc cgcttcaaac cgtttatcaa tgaagcgtta accgcaaagg gcttcaaaca
    18781 gcccacagag gtccaggaac gtttgattcc tttaattgca aaaggaaaaa atatcattgg
    18841 gcaatctcaa actggtacag gaaaaacaca tacgtttttg ttgcctttgt ttgaaaaaat
    18901 caatgcagaa aaagaggaag tacaagttgt tattacagct ccaagtaggg aactagctgc
    18961 acagatctat gaagcagcta cacaaatcgc taaattttct acaccggaga tccgtgtctc
    19021 gaattttgtc ggcggtacag acaaacagcg tcaattaaat cgtctgcatc accaacagcc
    19081 acaggttgta gtaggcacgc ctggtagaat attagacatg atgaatgaac aagcgctgaa
    19141 gattcacacc gcatttgcat ttgttgtcga tgaagcggat atgacattgg atatggtttt
    19201 tttaaatgaa gtagaccaaa ttgcaagtcg attaccagaa aagctgcagt ttttagtttt
    19261 ttctgccact attccagaaa aacttcgtcc attcttacga aaatacatgg aaaatccaat
    19321 cattgaagaa atcaaaccaa aagcagttat ttctgaagac attgaaaatt ggctgatttc
    19381 aacaaaaggt aaagatgaga atcaaatgat ttatcaactg ctgacaattg gtcatcctta
    19441 tttagcaatc gtatttgcca atacaaaaca gcatgtggat gaaattgccg attacttgaa
    19501 aaaccaaggc ttgaaagtag caaagattca tggagacatc accccaagag agagaaaacg
    19561 agtgatgcga caagtacaga atctggagta tcaatatgtt gtagcgactg atttagctgc
    19621 tagagggatc gatatcgaag gtgtctctca tgttattaat gcagaaattc cagaagatct
    19681 agacttcttt atccatcgtg tcggacgtac tggacgtaat ggcttaaaag gaacggccat
    19741 tacgttatat tcgccaaatg atgagcaagc cattgatgcg atagaaaaat tgggcgtctc
    19801 atttgaacca aaagaaatca aaaacgggga aatcgtcaaa acatacgatc gtaatagacg
    19861 tgccaaacga gaaaaatcaa aagatgtatt agatcccacg ctgattggtc ttgtgaagaa
    19921 gaaaaagaaa aagatcaagc ctggttataa gaaaaaaatc gattgggcaa ttgcagaaaa
    19981 aaataaaaaa gaacgcaaaa tcgaacgcag acagcaaagc cgtactgctc gtaaaaataa
    20041 gaaaaattca cataaataaa aaacaagagg ttgtgacaaa aggcgtgtca caatctcttg
    20101 tttttaagct agactttttt tcattccgat acaaagtact tcatcttcaa taaaaggttc
    20161 tgtgcaagta tgataccctt gtttttcata aaaatctaca gccgtcattt cagctactac
    20221 ataactagtc tgacaacctt ctgaaaccgc ccgtttttca gcagcttcaa gcaactgacc
    20281 gcctaaccct tttcctcggt attcttttgc tacgcaaaat cgttccggct tgaagcaagt
    20341 aggtgttttc ttttcataac gcaaagtagc tactggtctt ttttgatcaa gaaatagata
    20401 ataggtttgt tgcgggatat cttttccgtc gaattctagt tctgcaggaa tcccttgttc
    20461 taatacaaaa acttgatatc tcaaaaagca gctagctgcg cgtatccatt catccgctcc
    20521 aactattttt ttcatcatat tcctccttgt catgaatagc agagatgtat tgattccttt
    20581 attcttatcg taccaaaaat cgtggagtat gaccaaaagg aaatgaaaat aaaaattgag
    20641 atattgacag tgatcaatag aatgcgtata ctaattaagt acgaaaagga tatgatttgt
    20701 tttttaagac tcacagaaaa ttctttcttg gctgaaaaaa gaatagtcac taatcaaatt
    20761 gctccctttt tctattttgg tagaaatacc gacgcagctc agcgttatcg agtttaagag
    20821 gtaatgatta cacatcattg caatcaaggt ggtaccgcga gttttcgtcc ttggctgtga
    20881 tggtgttttt ttgtatttaa tttatagaaa gcaggtaatg acatgaaaca attaagtagt
    20941 gcacaagttc gacaaatgtt tcttgatttc ttccaatcta aaggacatac gattgaacct
    21001 agtgcgtctt tgatccccgt gaacgatcca accttattgt ggatcaactc tggtgttgca
    21061 acattgaaaa aatattttga tggttcagtt gtgccagaaa atccaagaat cacaaatgcc
    21121 caaaaatcta ttcgaacaaa tgatatcgaa aatgtcggta aaacagcacg gcaccatacg
    21181 atgtttgaga tgctgggtaa tttttcgatt ggtgactatt tcaaaaatga agccatccac
    21241 tgggcatggg aatttttgac tgatccaaaa tggatggcat tcgatccaga aaaactttat
    21301 gtaacagttt atccaaaaga tacagaagca aaacgcatct ggcatgaaga agttggcctg
    21361 ccactagatc acattattga tgtagaagac aacttctggg atatcggtgc aggcccatgt
    21421 ggtccggata cggaaatttt ctatgaccgt ggagaagctt ataacgattt agcagaagac
    21481 gatcctgaaa actatccagg aggagaaaat gaacgttatt tagaaatctg gaacttagta
    21541 ttttcagaat tcaaccataa gcctgataac acatttgaac cacttcctca taaaaacatt
    21601 gatacgggga tgggattgga acgcatggtc tcaatcgttc aagatgcgtc aacgaacttt
    21661 gagacagact tgtttatgcc tattatccac gccgtagaaa agctaagcaa acaagtaaaa
    21721 tatggtgaaa acccagtaac agatatttct tacaaagtaa tcgcagatca tattcgcgcc
    21781 ctttcatttg ctattggcga cggtgcattg ccatcgaatg aagggagagg gtatgtctta
    21841 cgccgtttgc ttcgtcgtgc tgtcatgcat gggaaaaaat taggtattga acaagcattc
    21901 ttatatcgtt tagtccctgt tgttggggaa atcatggtaa gttattatcc tgaagtactt
    21961 caacaaaaag aatttattga aaaagttatc cgtacagaag aagaacgttt caatgaaaca
    22021 atcaatgaag gtttggaaat cttaaatcaa gtgatccaag aagtaaaaaa cgaaaacggg
    22081 acgactctag caggaaaaga catcttcaaa ctatacgaca catatggctt cccggtagaa
    22141 ttaacggaag aggtggcaga agatgaagga ctgaaagttg atcatgctgg ttttgaaaaa
    22201 gaaatggatg cacaacgcga acgtgctcgc tctgcccgca gtactgaaaa atcaatgggg
    22261 gtacaatctg ctttattgac agatatcaaa gtagaaagta aattcattgg atacacagaa
    22321 caagaatcag atagtcaatt attggtcatc gttaaaggtg agtctctttt gaatgaagtc
    22381 gcagaaggtg aagctcaaat gatctttgat caaacacctt tctatgcaga aatgggtggg
    22441 caagtagcgg atcaaggatg gatcatgaat gaagtaggcg aaatcgtagc agttgttaca
    22501 gatgttcaaa aagcgcctaa tggtcaattt atgcacaccg tagaagtaaa agcaccaatg
    22561 agtgaaggac aaacctatca tctattgatc gatcaatcga tgcgcaatcg gatcatcaaa
    22621 aaccatacag caacccatct actgcacaag gcattgaaag aagtacttgg cgagcatgca
    22681 aatcaagctg gttctttagt cgctcctgga catttacgat tcgactttac ccactttggt
    22741 caaatcactt cagatgaatt agctgaaatg gaaagaatcg tcaacgaaaa aatctgggaa
    22801 gctatcccag ttgaaacaat tgaaacagat atcgatacag ccaaaaacat gggtgcaatg
    22861 gctttgtttg gtgaaaaata cggacatgac gttcgagtag taaatattgg cggctggtca
    22921 atcgaactgt gcggaggcac acatgtagca aatacagaaa acatcggaat cttcaaaatc
    22981 gtttctgaat caggaattgg tgcaggcgtg cgacgaatcg aggctgtaac aagtaaagaa
    23041 gcgtacgaac tacttgaaga agaagaaaaa caactaaaac aagtagcaac aattgttaaa
    23101 tcacctcaat tgaaagaagt tgtttcaaaa gcggaacaat tgcaacagca agtaagagag
    23161 ctacaaaagg aaaatgaaca attagctagc aagttagcga atcaacaagc tggtgatgta
    23221 ttcaaagatc cagaaacgat caatggaaaa acaatcattg cagcacaagt aaaagtgaaa
    23281 gatatgaatc agctgcgtca attagctgac caatggaaac aaaaaaatat ttcagatatt
    23341 cttgttttag caaacgaaca agaaggaaaa gtcagtctgc tagcggcaat gacgaaagaa
    23401 tcaaatgaag ctggactaaa agctggcgac ttgattaaag caatcgcacc gaaagtaggc
    23461 ggtggcggtg gtggacgtcc agacatggca caagctggcg ggaaaaatcc tgctggtatt
    23521 ccagatgcac ttaaagctgc gaaagaatgg gttgcgcaac aatagggcgt ttacgtttga
    23581 cagaaaaaca aaagttcaaa cattgaacga aacatgatta tgggggcgaa gacaaacaag
    23641 tcttgccctc atactttttt caattggatg gcgcatctaa tcaaatggtc ggattcggat
    23701 ggtctttagt tgacgaaagt gacagaaatt tttacgatta gtataaataa aaaggggtga
    23761 aaaaatgaat agtagaaata agacctttcg actagtctta cgtgcgatct tgttagcaat
    23821 catcatcgta caagcgatgg ttccttggct ggggtttatt ccattaggat tcattagttt
    23881 aacaattatc catatcacag taattattgc tgcagtagta ttaggcccaa aagatggcat
    23941 ggtgattggc ttgttttggg gaatcgctac aattgttcgt gcctatgcca tgccaacaac
    24001 gccatttgat acattagtgt tcacgaatcc gattatctcg gtagttccca gagtacttgt
    24061 tggcttagta gcaggcttgg ttttccactg gatttatcaa aaaaataaat caataacgat
    24121 cagctcagcc tttgcagggg tactgggttc attggtcaat acagtcttag tgttaggatt
    24181 catgggatta ttttatacag gtgcaacggc agatgcctat ggtgtagact ctgctctttt
    24241 atttaaaacc ttagcagggg ttgcagcaat caatgggata ccagaagcaa taggtgcggg
    24301 aatcataaca ccattactag caaaagcgtt atttgcagca acaccgttga aaccagaata
    24361 atgaccatct tttaatagta ataggacgca cgatagatag aaaaaggaag agaggagagg
    24421 aaaacaatcg tcctctccat ctttttacct gagcaacaag caaactagaa ggggtaaaaa
    24481 acttggctaa gaaacaagaa gaaaaaaaga aacaaatacc gcaacagcgg acaaaaaaag
    24541 gagtactagc gggtatctta ggattggtta cgaatctgat cttgtttgtc tctaagctaa
    24601 tgatcgggct tttttcagga agtgtctcca ttatggctga tgcagtgaac agtctatcag
    24661 atactgcttc atccatattg actttgatcg gatttaaaat tgctgcaaag ccagcagatc
    24721 aggaacatcc ttatggacac gaacgcttcg aatatatcag tgggttattt gtttcgatca
    24781 tcattactta tgtaggattt caatttttag agtcttctgc tgaaaaaatc atccatccag
    24841 aaaatgtctc tttaacaccc atcgtctttt ttgtcctaat catttccatc tttttgaaat
    24901 ttgcacaagg caaaatgtat caggttattg cgcaatccat tgattctgaa acattgagag
    24961 ctacagcaac agatagttat aatgatgtct ttactacact ggctgtattg ctttcagcag
    25021 caatcgaatg ggtaactgga tggcgtattg atggatatat aggttttgct ttagcattat
    25081 atatccttta tagtggattg acgatgcttc gaaattttgt atacgaacta ttaggaagtc
    25141 gcccaacgcc agcagagatt gaagaaatgg aagagcattt aaatgcttgc gatgatatac
    25201 ttggctatca tgatttattg gtgcataact atggacccaa taaacgcttt gcatcagtcc
    25261 acatcgaagt agatgaatca atgaatttga atgtggctca taagattatt gatctgatag
    25321 aaaaagactt taaaaagtct ttggatgtgg aacttgtctg tcacttggat ccaatgcctg
    25381 ttgataacga aaaatactat aatatcttgc ggcagttaaa gcaaattctt cttgaaacag
    25441 gtacagggct taatattcat gattttagag taattcatca aggaaaaacg ctgcaatttg
    25501 atgtagtggt ccctgagaaa ttccagtttg ttgatacaga actagaaaga atcttacgta
    25561 gtcaaataga agaaaaaatc ggagattacc aattggaaat tacgtttgat cacaattatc
    25621 ttttatatta aatgagaaac tataaaaaga ctccttaaag tttttaattt aaagagtctt
    25681 tttttctttt aattgtttat ggagagcctg acgtgcaaga ttatctgctc ctttattttt
    25741 gttttcgggc aaccattgca ggatcaataa agaaaattcg gatagaagtt cttggattct
    25801 ttgaaggtag ggaaggaaga gctcgttctt cgttgcgttt ttgtcgaccg tagaaactag
    25861 tgttttgctg tctgaaaaac agaaaatcgt ttcattctgt aagtcgtttt tctttagaaa
    25921 ttctaaagtt tttaaaaaga cttcgaactc tgcttgatgg tttgtcccta tagaaagcgg
    25981 gatagatagc tgttcttgtt taccttgata gacaatcaag attccacctc cactaggacc
    26041 agggttgcct tttgtagagg catcaatgta tacttttaac ataaaaagag attctccttt
    26101 ttgttcaaac taatgttatg gtaagattat atcattactg aatcggtgag aggttagaga
    26161 atgaaaaaaa atattcgatg gcagcctgaa ctggcacata cgatcatcta ttggtcattg
    26221 accttgatca ttctgttttt tagtctgatt ctttctttag aaaacacagg tccttattgg
    26281 aaaagcaatt ttgttttagg tatttttctt gtttttctat ttctaggttt gcggcgttct
    26341 tttattttaa aatcaaactc tgtgattata cgttatgctg ctttttggaa agaccgagaa
    26401 atatctgtcg gacagattcg atctttgacg ccaactaata ctggggtgca aatgatgttg
    26461 caagatgaga aagccccatc tgttttcatg atgcgaaaaa aagatcaagt agtccttttt
    26521 gatcattttg aaaaaaacgg acccaaatca gaatcaattc cttatcctta tacgaaaaaa
    26581 agaaaaagct aattttaaaa atcagaacct aaaaaaacga atttttacag gaaaagaaaa
    26641 gagtgagacg tacggttcat cccgtttagc gtctcactct atttgtttat ctattcatgc
    26701 atactagtat cggtagtctg aacgttgcct ttcttgacat gggcagcttg tgttcctcga
    26761 ttcccttcga tgatctcaaa ttcgaccact tgattttctt ccaaagtttt gaatccctct
    26821 tcttcaatcg cagtaaaatg tacaaaaatt tcttctgtgt catcataact gataaagccg
    26881 tatccttttt tgttgtcaaa ccattttaca attcctgtgg tcaaagcact cacgaccttt
    26941 ctaaagtctg atcatagcct tcattataaa gagtatttat aagtacgtca aataaattaa
    27001 aaacctgtaa ttttatgttt tttttctgaa taaaaaaagg tataattggc atatcttaac
    27061 aaagaagata ttttgttgaa taaagttcgt gaaaaggggt aatcaaatga agaatgatct
    27121 gcaaatttca caagaaacag agctagaacc gattgttgaa gtggctagta aattagatct
    27181 taaggaagac gatttggaac tgtatgggaa atataaggcg aaaatcaatt tttctggaat
    27241 caatcgatca aaggataacg ctgaggggca tttgatctta gttacttcca tcaatccaac
    27301 accagctggc gaaggaaagt caacgatcac tgtagggtta ggagacgcgc tcaataaaat
    27361 caataaaaaa tctgttattg cattgcgaga accctcactt ggtcctgtga tgggaatcaa
    27421 aggtggtgct gcaggcggag gatatgcaca ggtattgcct atggaagata tcaacttgca
    27481 ttttactggt gacatgcatg ctatcacaac agctaataat gcattatctg ctcttttaga
    27541 taatcatatt catcaaggaa atgaattgaa catcgatgcc cgccgagtga tttggaaacg
    27601 ggtagtcgat ctcaatgatc gtgaactgcg taaagtaatt gttggcttgg gtgggccaat
    27661 ccaaggtgtt ccaagagaag atgggtttga tatcacagtt gccagcgaaa tcatggctat
    27721 cttgtgtctt gcggcagacc ttgaagattt aaaggctcgt cttgcaagga tcgttgtcgg
    27781 ctatacattt gaccgtcagc ctgtgactgc tggagacttg aaagcagaag gcgctctggc
    27841 attactgctg aaagatgcaa tcaagccgaa tcttgtacaa acaatttacg gaacaccagc
    27901 ttttgtccac gggggaccgt ttgccaatat cgctcatggc tgcaatagta ttttagctac
    27961 caagacagca ttgagattag cagattacgt tgtcacagag gcaggatttg gtgcagatct
    28021 tggcggagag aaatttttag atatcaaagt acctaacttg aaaaaagcgc cggatgctgt
    28081 ggtgattgtc gccactgtcc gtgcattgaa aatgcatggc ggaatgaaaa aagatgaact
    28141 taaaaatgag aatcttgatg ctttgaaagt aggttttgca aatttaaagc gccatatacg
    28201 taatatggaa caatatcagc tacctgtgat cgtcgcaatc aatgaattcg taacagatac
    28261 agacaatgaa ctcacactgt tggagcactt gtgtgaaaat caggggattc ttgccaagag
    28321 agcgagtgta tgggcagatg gagcagaagg cggcgttgat ttagcaaaag ctgtagtacg
    28381 attgattgat agaaaagaag ctaattacaa gccattatac cgcctagaag aaacgataca
    28441 agaaaagaca gaaacaatcg tcaagaaaat ttacggagga aatggtgttg ttttcagcaa
    28501 aaaagcgcaa aaacaaatag aagagtttac gaagaatgga tgggacaaac tgccgatttg
    28561 tatggcaaaa acacagtatt cattttctga tgatccttca ctgctaggtg caccggaaga
    28621 tttcaccatc acgatccgcg aaatcatccc aaaattaggg gcaggatttt tagtagcctt
    28681 gactggagat gttatgacga tgcctggttt gcctaaaaaa cctgctgcat tgaatatgga
    28741 cgtgacaaat gacggagaag tgttgggatt gttttaagct tgattataat ctaccatcga
    28801 aggagctgta ttttgactac agcttctttt tttagacgat agggaattaa ttttgtttac
    28861 actaggatca ttctttttct ttattgtatt ttgagctata ctaaagcata ataggagggt
    28921 atgatgaaaa cgacatttat aagaaaaacc ggtttttacg gtatgggtag ctcgattcag
    28981 ctaagaatca acaatgaaaa acgcagtgtt ctcagtcatg aacaaaagat aacgcttgat
    29041 ctggaagtgc cctttacgat gcaggtcacc ttttattggc taaaaagccc cgtttataca
    29101 gtgaaagaag caaagcctgc ttatgtgatc acaatgaatt acttgattct tcaaatctat
    29161 ccgtttttat ttctagcaac tgggttgggg gccgttttcc tacaaaattt atttttcagt
    29221 ttttttgcag ttattgtgtt gattgcgttc tttttctata taaaaaaccg ggcatatttg
    29281 atcaaggagg aaaattatgg caaactctga tcttttttta atcagtttca atcgaatcga
    29341 aaaatggatg agagacgaga tgggaaatgc acgaaatatg ggatttaccg aacttgtcag
    29401 acgcctagct caaagaaaac aattaatgat cagaaaatac gaagatgatc ttttacagct
    29461 tgctcagtta aggaatgcga tcgtccatga tcggatcgca gtcgattttg tcattgcaga
    29521 gccaaatgat tggacagtca agcgaatcca gctgatcgaa caagaactga ttcagcctga
    29581 aaccgtgctg cctcgatttg caaaacgtgt aacaggtttt gaacaagatc ttccgttact
    29641 tgaactgtta aagatagtag cagaaaaaag gtattcacaa tttccgttat acaataaagg
    29701 aaaatttgta gcattgatta ctttacgtaa tatcgggttt tggctagcaa aagaaagcca
    29761 aaaaggtccg gttgatttaa caaataaacg agcgttggat ttgatcatcc aaaacggaaa
    29821 gtacacaaat tatcattttg ttccagctaa aacacatatt tacgaggttg aagcgatgtt
    29881 tcgtgagcaa gggactttgg aagccgtact tatcactaaa gatgggaatc ccgatggtaa
    29941 tctgctcggg atcgtacgac ctcgtgatat ttataaacaa atagaaaagg attgattttt
    30001 tgttaattat ttattggata ttcagcgctt tgctagtagg agtggatcag ttggtcaaat
    30061 ggtggatcac tgataatcta gcgcttggag agacgaaaaa tcttatacca ggtattttgt
    30121 cactgaatca tattcgaaac actggagccg catggagtat gctagaaggg aagatgtggt
    30181 tctttactat agtgacactc attgcagtcg ttgtgattct cacattgatg attaaaaatc
    30241 gtgaaaaagg taatcgctgg ttcatgattg gattgagttt gattctagct ggtgcgattg
    30301 gcaattttat cgaccgtatt cgtcttggct atgtagtaga tatgttccag acagatttta
    30361 tgagctttcc gatttttaac gtagcagatg tgacacttgt atgtggagtg atttgtatgt
    30421 taatttatat tattttagat gaaaaggatc aaagaaaaaa atgacagaac ttaaaacgac
    30481 gattcaccat gaatctggta ggatcgataa agtattgact aatttattca ccgattacag
    30541 tcgttcgcaa attcagctgt ggctgaaaaa tggagcggtt tctgtcaatg gacagccagt
    30601 caaagcaaat tacaaagtaa aacaaggaga cgagattact gtcttgatcc ccgaaccaga
    30661 aacattagat gtggtcccag aaaatatccc gttgacgatc gtttacgaag atgaagatgt
    30721 agcggtgata aacaaacctc aaggaatggt cgttcatcct tcagcaggtc atacgcatgg
    30781 cacaatggtc aatgcgctga tgtaccatat caaggatctt tctacgatca acggcgttat
    30841 tcgtccaggt attgttcacc gaatcgacaa agatacttcc ggactgctaa tggttgccaa
    30901 aaatgaccag gcacacgaat cattagctca gcaattaaaa gataagacct ctttgcgcaa
    30961 atatgttgct ttagtccatg gagagattcc tcacgaaaaa ggaaccatca atgcgccaat
    31021 cggccgttcg aaggaaaacc ggaaaatgca ggcgataaga gaagatggga agtcggctgt
    31081 cacacatttt actgtattgg aacgtttcga aggatttact ctggttgaat taaaactaga
    31141 aaccggaaga acacaccaga tccgtgtgca tatgaagtat atcggttatc cgttagctgg
    31201 agatccagta tacggaccga agaaaacttt gaaaggcaat ggtcagtttc tccatgcgca
    31261 aatgttaggt tttacgcacc cgactacaaa cgaaaagatg atctttgaag cacctcttcc
    31321 agaagttttt gaaaaaactt tagaaaaatt aagaaaaaac attgcatttt aatttagata
    31381 acggtatagt aattaacaac gaaagcataa aacttaatag aattccttta agtttagtcc
    31441 cgtgaggcta agaagggtgg gagcaaggat acgtatggtg cgccataagt agattgctca
    31501 ttgacatagt atagctatca gtgtatcagt atacgctcag tctaaacatt cctcctgtcc
    31561 tcttagggca ggagtttttt tatgcaaaaa agtaggggga atgaaaatgc aagcaaaaga
    31621 agtagtagat caggtaacca tgaagcgagc attgactcga attacttatg agattatcga
    31681 gagaaaccat agtatccaag atatcgtatt ggtaggtatc aaaacacgag gaatctacat
    31741 tgcatcaaga attgccgaac gcctgaagca gctagaagat atcgatattc ctgtaggaga
    31801 actggatatc actttatacc gagatgacaa aaaagaaaat ccagaagaac cggaacttca
    31861 ttcttctgac atcccagttt ccttagaagg aaaagaagtt attttgattg atgatgtttt
    31921 gtatacagga cgaacgattc gagcagcaat ggacgcagta atggattttg gcagaccaag
    31981 aaaaatttca ttagcagtcc tggtcgatcg tgggcatcga gaattgccaa tccgtgcaga
    32041 ttatgttggg aagaacattc caacatctcg ggcggaagag attttagtag agatgcaaga
    32101 gcttgatgga caagatcgca tcatgatttt gaaagaggaa gattagagga gagagaaaca
    32161 tgtcggagaa aaaaggattt cataatgatg acgtggtgtt agatatccac gatcgcccaa
    32221 ccccaggaca ttgggtagga ttaagtttac aacacttgtt cacgatgttc ggagcaaccg
    32281 tgttagtccc catcttagta ggtatcgatc caggtattgc gctagtcagt tcaggactag
    32341 gtacattggt gtacttattt actactaaag gaaagatacc tgcgtactta ggaagcagct
    32401 tcgctttcat tgcagcgatg cagcttttga tgaaaacaga tggttatcca gcgattgcac
    32461 aaggagcagt cacaactgga ttagtctatt tgattgttgc gctgatcatc aaaaagatcg
    32521 gttcagcatg gctggataaa atcttgcctc caatcgtcgt cggtccagtc atcatggtta
    32581 tcgggttggg acttgcggca aatgcagcaa caaatgctat gtataatcag ggcacgtatg
    32641 atttcaagta tattgctgta gcgctgatca cattaggatt aacaattttc ttcaacatgt
    32701 ggctaagagg ttttctcgga ttgattccga ttttactagg tattattagc ggatacatcg
    32761 tagccttgct gtttggaatc gtggatacta agccaatcgt agacgctgcc tggtttgctg
    32821 ttcctaactt tgagattccc tttgtacaat atgaaccaaa gctatatctt ggagcaatca
    32881 caacaatggc acccatcgct tttgtgacga tgacagagca tatcggccat cttatggtat
    32941 tgaataaact aactaaacgt aatttctttg aagaaccggg tcttcataag acgttgacag
    33001 gtgatggatt agcacaaatc gtggcagcct ttgtcggagg tcctcctgta acaagttacg
    33061 gtgaaaacat tggtgttctg gctatcacac gtgtccatag cgtgtttgtg atcggtggag
    33121 ctgcagtatt tgcagtcatt ttaggattcg taggaaaatt aagcgctttg attttaagta
    33181 tccctggacc agtcatctca ggaatcagtt ttatcttatt tggagtaatt gccgcaagtg
    33241 gaatgaagat tttaatcgag aacaaaatcg attttgataa aaagaaaaat ctgttgattg
    33301 cttcagttat cttagtagta ggaattggcg gactcgtctt tgaagtaggc acatttacat
    33361 tgtcggcaat ggcattagca actgtacttg gcatcatttt aaatcttatc ttaccagaaa
    33421 cttcacgaag cgaagaaaac taaggaggaa acattatgat catcaaatca gaacgtatca
    33481 gcttaaagca tttattgacc gttgaggcac ttactgatca agaagttatg ggcttgatcc
    33541 gcagagggca ggaattcaag caaggtgcta attggactcc gcaaaagcgc cagtactttg
    33601 ctacaaacct ctttttcgaa aatagtactc gcacacataa aagcttcgat gtagcggaga
    33661 aaaaactagg gctagaagtg attgaatttg aagcaagcac aagctctgtc caaaaagggg
    33721 aaacactata cgatacagta ctgacgatgt cagcattagg cgtagacgta gcggttatcc
    33781 gtcatggtga tgagaactat tatgacgaac tgatacaaag taaaacaatc cagtgttcga
    33841 ttatcaatgg aggagacgga agcggacagc atccaaccca atgcttgctt gatttgatga
    33901 ccatttatga agaatttggt cattttgaag gtctgaaagt tgccatcgta ggtgatatca
    33961 ctcactctcg cgtagctaaa tccaatatgc agatgttaaa acgtctaggc gcacaagtat
    34021 tcttctcagg tcctagagaa tggtacgacg atgaattcga agtgtacgga cattatatgc
    34081 cattggatga actactagat caagttgatg tcatgatgat gcttcgggtg caacacgaac
    34141 gccatgatgg aaaagaaagt ttctcaaaag aaggctacca tcaggaacat gggttaacag
    34201 ttgagcgtgc gaagaagttg caaaaacatg caatcattat gcacccagca ccagttaacc
    34261 gtgatgtaga attagctgat tcattagtgg aaggattaca gtcgcggatc gttcagcaaa
    34321 tgagcaatgg agtattcgtc agaatggcaa tcttagaagc agtgctaaat ggaaaagcgt
    34381 aattatagga ggatgaaaaa atgaaaacac tcattaaaaa cggtcagatc aatacacgaa
    34441 aaaatatgac gacaccagca gaaatttgga tagaagatgg gcgaatcaaa gcaatcggta
    34501 caggattctc agaagccgaa tttgatgaag tgtttgatgc aaagggacaa ttgatcacac
    34561 ctggtttagt ggacgtccat gttcatttga gagagccagg atttacatac aaggagacga
    34621 tcgaagctgg gactcgctca gctgctcgtg gcggtttcac tactgtatgc gcgatgccga
    34681 atttaaatcc tgtacctgat acggcagaaa agctgagaca agtatatgac atcattagaa
    34741 aagatgcagt tgtgaaggta ttgcagtatg cgccaatcac agaaaatctg cgaagtgaga
    34801 aattagttga tcaagaagca ctgattgaag aaggtgcttt tgctttcaca aatgacggtg
    34861 taggtgtcca aacagctggg acgatgtatc tggctatgaa agaagctgca aaaaacaata
    34921 aagccctagt agcccataca gaagatgaat ctttattatt tggcggcgtt atgcacgctg
    34981 gcaagaaggc agaagaactt ggtcttcccg ggatcttaag cgtaaccgag tcttctcaaa
    35041 ttgctagaga tctgttgcta gctgaagcaa ctggtgttca ttaccatgtt tgccatgttt
    35101 ctactaaaga aagtgtccgt gtaatcagag acgcaaaaaa agcaggaatc cacgtaacag
    35161 cagaagtttc accccatcat cttattttga tcgacgaaga tattcccgaa gatttcggct
    35221 tttggaaaat gaacccgccg ctaagaggac gggaggacag agaagctttg atcgaaggct
    35281 tgcttgacgg tacgatcgat tgcatcgcta cagaccatgc cccccatgga ttagaagaaa
    35341 aaagccaaag tttcatgaaa tcaccatttg gtatcgtagg aagcgaaaca gcgtttcaac
    35401 tgatctatac tcattttgta gaaactggtc gattcacgtt ggaacaagtg atcaattggt
    35461 tggcagttaa accggctgag atttttggtt tgaatgcagg aacgctaacg gttggtgccc
    35521 ccgctgatgt agcagttttt gatattacac aaacttgcac gatcgataaa gaagactttt
    35581 tatcaaaagg ggagaataca ccttttatcg gctggaaagt aaaaggcgaa acacaaatga
    35641 cttttgtcaa cggaaaactt gtgtggcaga agggagaata aaagtatgga acgtttgctt
    35701 atattagaag atggaacagt tttcgaagga aaggcttttg gtgcagatgc tagtgtcttc
    35761 ggagaaatcg tttttacaac aagtatgact ggttatcaag aaactatcac tgatcaaagt
    35821 tttaacggtc aaatcattac atttacctat cctatggtgg gtaattatgg ggtaaataga
    35881 gatgattatg aatcgatttc tccaacatgc aaaggagtag tagtaaaaga acatgcacgt
    35941 gtagcttcaa actggcgaaa ccaaatgacg ctagattcat ttttgaaaca aaaaggaatt
    36001 cctggaatat caggtatcga tacgcgcgcc cttactcgca aaatccgtga agtcggaacg
    36061 atgaaagcta gtatagttga tgctggagat tccttcgaac atgcatttga tcagctgaaa
    36121 gcaaccgttc tagcaaccaa tcaagtgcag caagtctcaa ctaacaaacc ttatccaagt
    36181 ccaggaacag gtcggaaagt cattgtcatt gacttcggct tgaaacatag tattttacga
    36241 gaattgagta aaagaaactg taacctcaca gtacttccct ataacacaga tgccgagacg
    36301 attttagcgc tatcaccaga cggagtcatg ctgacgaatg gacctggtga tccaaaagat
    36361 gtgccagaag caatcgaaat gatcaaaaaa atccaaggaa aagtaccgat ttttggtatt
    36421 tgtctcggtc accagttatt tgctttagcc aacggtgcag atacctataa aatgaaattc
    36481 ggacatcgag gattaaatca cccggtacga gaagtagcga ccggaagaat tgattttact
    36541 tctcaaaatc atggctatgc agtcgatgaa gccacgattg atccagaaaa actcatcgta
    36601 actcatgtag aagtaaatga cggaacagta gaaggagtca aacacagaga ttatcccgct
    36661 ttcagtgtgc agtttcatcc agatgctgcc cctggtccgc atgatgcact acatttattt
    36721 gatgaattta tggaaatgat ggacgcagga aaggagcagt aaacagatgc cgaaacgtac
    36781 agatatcaag aaaattatgg tgattggatc tggtccgatc atcattggtc aagccgcaga
    36841 atttgactac gcaggaacac aagcctgcct tgccttaaaa gaagaaggat atgaagtggt
    36901 tttagtaaat tctaatcctg cgacgatcat gacggataaa gaagtggcag atcaagtata
    36961 tatcgaaccg atcacacttg aatttgtatc aaggattctt cgaaaagagc gtccagatgc
    37021 tctccttcca actttaggcg gacagacagg cctcaatatg gcaatggagc tggcagcatc
    37081 aggtatttta gaagaattga acatagaact tctaggaaca aagctttctg cgatcgatca
    37141 agcagaagat cgtgatctat tcaaacagtt gatgaaagaa ttgaatcagc caatccccga
    37201 gtctgaaatc gtcaatacag tagaagaagc tgtagatttt gcaaataaaa tcggctatcc
    37261 tgtcatcgtt cgtccagcct ttactttagg cggaactggt ggaggtatgt gtgaaaatga
    37321 agaagaatta cgtatcgtat cagaaaatgg attaagtctt tcccccgcta cccaatgttt
    37381 gattgaacga agtattgcag gttttaaaga aattgaatat gaagtgatga gagattcggc
    37441 tgataatgca atcgttgtct gcaatatgga aaattttgac ccggtgggta tccatacagg
    37501 tgattctatc gtatttgccc ctagtcaaac attgtctgac catgaatatc aaatgcttcg
    37561 tgatgcttct ctttcgatta ttcgagcact taagatcgag ggaggctgta atgtccaact
    37621 cgctcttgat ccacatagtt ttaattatta tgtgattgaa gtcaatccgc gtgtttctcg
    37681 ttcatctgcg ttagcaagta aggcgactgg ctatccgatc gctaagctag ctgctaaaat
    37741 agcgcttgga ttaacattag acgaaatgaa aaatcctgtt acaggaacga cttttgcaga
    37801 atttgaacct gcattagatt atgttgtagc taaaattcct cgctggccgt ttgataagtt
    37861 tgaaaacgga gaacggcttt taggcacgca gatgaaagca actggtgaag tgatggctat
    37921 cggtcggaat atcgaagagt ctttattgaa agctgtgcgt tctttagaaa tcggtacaca
    37981 ccatatcgaa cttcctgagt tggcagacat cacggacgat gacctaatcc aaaaagtggt
    38041 tcgagcacaa gatgatcgcc tgttttatgt agctgaagct attagaagag ggtacaccat
    38101 agaagaatta gcggatttga caaaaatcga tttattcttt ttagataaac tgttgcatat
    38161 cgtagaaatc gaacagcagt tgaaacagca gccgttggat gacaagatat tgaaaacagc
    38221 gaaacaaaac ggttttacag acaaaaaagt ggcggagctg tgggaaataa acgaagaagc
    38281 cgttcgccag atgagaaaag aaaatgattt gattcctgta tataagatgg ttgacacttg
    38341 tgcagcggaa ttcgaatcaa aaacacctta tttttatagt tcctatgaat atgaaaatga
    38401 aagttcacga tcagaaaagc cctcaattct agtactggga tctggtccta tccgtatcgg
    38461 acaaggggtt gaatttgact atgcgactgt gcattctgtc aaagcaatcc agcaggcagg
    38521 atacgaagca atcatcatga acagcaatcc tgaaacagtt tcgactgact tttctatttc
    38581 ggataaatta tattttgagc cattgacctt cgaagatgtg atgaatgtga tcgagctaga
    38641 acagccgata ggagtaatcg ttcaatttgg tggtcagaca gcaatcaact tagccgaacc
    38701 tttgactgct gctggtgtaa aaatcttagg gacaacgatc gaagacttgg atcgtgcgga
    38761 aaatcgtgat ttattcgagc aagcgctgaa agagttggat attcctcaac ctttagggga
    38821 tactgctaca agtaaagaag aagccgtggt tatcgcttcg agaattggtt atccagttct
    38881 tgttcgtcca agctatgtat taggcggacg tgcgatggaa atcgttgaaa accagcgaga
    38941 tttagaagat tacatggaac atgctgtgaa agcatctcca gaacatccag tactagtgga
    39001 ccgttacttg cttggtggtg aatgcgaagt ggatgcgatt tgtgacggtg aaacagtact
    39061 catcccaggg atcatggagc atattgagcg tactggtgtc cattcagggg attcaatggc
    39121 tgtgtatcca cctcaaacat tatctgaatc gatcaagaaa acgatcgaag attacacgat
    39181 ccgcttagca ttaggtttga attgtgtcgg gatgatgaat atccagtttg tcatccatga
    39241 aaatgaggtt tatgtgatcg aagtcaatcc tcgagcaagt cggactgttc cattcttaag
    39301 taaggtgaca ggaattccaa tggcacaaat tgcgacgaaa gctattttgg gagagaaact
    39361 aaagacactt ggttatgaaa acggacttta tccagaaaca aaagaagttc atgttaaagc
    39421 acctgtcttt tcttttacga aattgcagaa agttgatact tacttagggc ctgaaatgaa
    39481 atcgactgga gaagtaatgg gatctgacag acaattagac aaagctttgt acaaggcatt
    39541 cgaagcttct ggattacact tgcctgaata tggagccgta ttatttacga ttgcggacga
    39601 aacaaaagaa gaagcactag aattagctaa acgttttaca gaaattggct atagtctgat
    39661 aggaacgaaa catacagctg cttatttcga aaaaaatggt ctggtcgttg aaacagtagc
    39721 gaaaatttct gaggaagcag cagaaaaaaa tgtagtcgac ttaataagag atggtaaaac
    39781 acaagtggtt gtcaatacga ttgataaaga tcggggaaat gcttctaaag acggtttcat
    39841 catccgaaga gaagcagttg aacatggtat tccattgttt acttctttag acacagcaga
    39901 tgcgatcgtt cgagtgatgg aatcccgtgc tttttcaact aaccctattt aacggcgagc
    39961 acatgttaag acaaaaagac gaaaaaccgt tgcaggaggg gacagatgaa acaagaaatg
    40021 atggaaatcg ttcagcaaaa acagctggca ccaaagattt ttgaaatgac attgaaaggt
    40081 gatctagtaa aagaaatgca gcaaccagga cagttcttgc atattctcgt gccgcgtgca
    40141 gacttgctat tacgccgacc gattagcttg aatcatatcg accacgaaac aaatacttgc
    40201 cgtatcattt atcgggtgga aggtgagggg actagagtct tctctactct ttctgccgga
    40261 gaccatttag atgtcatggg tcctttaggc aatggttttg atttgtccaa tcttcataca
    40321 ggagacgaag tatttataat cggtggagga atcggtgtcc ctcctctata tgagttatca
    40381 aaacagttag tcgaaaaagg aattaagcca acgcattttt taggttttgc cacacatgaa
    40441 gtcgtttatt atgaaaaaga atttaatcgt ctaggagaaa caagaatcgc tactgacgat
    40501 ggaacatacg gtatgcacgg aaatgtcggt aatttattgt taggaacgaa gacggaacct
    40561 acagctgttt ttgcatgtgg ttcaaacggg ttattgaaga cagtggaaca gctgttttct
    40621 tcgcatcaaa atgtccagtc atcattagaa tcccgcatgg catgtgggat aggggcttgc
    40681 tatgcttgcg tttgtcatac gccggaagat gaaacaggaa caaaaagtgt gaaagtttgt
    40741 gacgaaggac cgatttttaa aataggagag gtaattatat gaatgagaat cctttagcgg
    40801 tcaaacttcc tggcctagaa ttaaaaaatc ctatcatgcc ggcaagcggc tgtttcggtt
    40861 ttggcaaaga atacggaaaa tactatgatt tgaatcagct tggatcgatc atggtcaagg
    40921 caacgactcc aaatgcccgc tttggcaatc ccacgccaag agtggcggaa acaccatctg
    40981 gcatgttgaa tgcaattggt ttgcaaaatc ctggtctaga cgttgttatg cataaacttc
    41041 ttccagaatt agaggagtac aatgagttac ccatcatcgc aaatgttgct ggtgcttgtg
    41101 aagaagacta tgtagaagtt tgtagcaaaa ttggcgaagc accaaatgtg aaagcaatcg
    41161 agctgaacat ttcttgccca aatgtcaaac atggcggaat tgcttttgga acagattctg
    41221 atgttgcttt tcaattaaca caagcagtaa agaaagtttc ttctgttcct gtctatgtca
    41281 aattgtcccc aaatgtaaca gacatcgttc caatcgctca ggcaatcgaa gctggagggg
    41341 cagatgggtt tacgatgatc aatactcttc ttggtatgcg gattgacctt aagacaagaa
    41401 aaccgatttt agccaatcaa acaggtggac tctccggacc agcaatcaag cctgtcgcta
    41461 ttcgtttgat tcaccaagta gcaagtatat ccagccttcc aatcattggg atgggcggcg
    41521 tgcagacagt ggatgatgtt cttgagatgt ttatggctgg agcaagtgct gtagctatag
    41581 ggaccgcgaa tttcaccgat ccgtatatct gtcctaaact aattaaagag ctccctgtgc
    41641 gaatgagcga actaggcatc gaatcgctgg aaagattaat caaagaagtg agagaggaac
    41701 gtgaacgatg aatcaactag accaacgacc aattattgca ttagattttt caactaggca
    41761 agaggtagaa gactttcttc gatttttccc gaaagaagaa aaattgtttg tgaaaatcgg
    41821 aatggaattg ttctaccaag aaggacctga aatcgtacgt tatctaaaag aggctggtca
    41881 taatgtattt ttagatctga aacttcacga tattccgaat acggtcgaaa aagcaatgcg
    41941 gggattggca aagcttggtg ttgatctcac ctgtgttcat gccgctggcg gtattcgtat
    42001 gatggaggcg gcaatgcgag gattagaaga ggggactcca gaaggtggaa aacgtccgct
    42061 acttctagct atcactcagc ttacttctac aagtgaagaa gaaatgcatg ccgatcaact
    42121 aatcgaagtt tctttagaaa aaagtgtgat ccattatgct agctgtgcca aaaaagcagg
    42181 attagatggt gtggtttctt ctgcttggga agtagaagcg atcaaagaaa tggcaggaga
    42241 cgaatttgtt tgcttaactc cgggtataag accagaagga acagtcgcag gagatcagac
    42301 aagagtagtc acaccttctc aggcaaaaaa gatcggctca acttttatcg ttataggtcg
    42361 accaatcact cagtcaacta atccttataa agcatatcaa acgattcaaa cagaatggag
    42421 ctaacctaaa atgaatgttg aacaaagtat cgcaaaagac ttattagaaa ttgaagcagt
    42481 atttttaaat ccaagtaatc cttttacatg ggcaagtgga atcaaaagcc caatttactg
    42541 tgataaccga atcacgatga gctatccaaa agtccgaaaa gagatcgcaa aaggattagc
    42601 aagcaaaatc aaagaagctt accctgaagt acaagtgatt gctggaactg caacagcagg
    42661 aatccctcat gccgcatggg tagcggaaat actggatttg ccaatggtct atattcgaag
    42721 taaggcgaaa gatcatggga aaggtaacca aatcgaagga cgtatatttg aaggtcagaa
    42781 gatggttgtg atcgaggatt tgatttctac aggcggaagt gtcttggaag cagctgaagc
    42841 agcaaaaaga gaaggtgcgg atatccttgg tgttgcggct atctttacgt atgagttgcc
    42901 aaaaggaaaa gcaaatttcg aaaaagccga aatccctctt atgacgttga ccaattattc
    42961 tgtattgatt gaagcagctt tagaagatcg ttatatcgat gagcaagaat taactctgct
    43021 aaaagaatgg aaaaaagatc ctgaaaattg gcagatttaa gcgcgcttga tgaagataga
    43081 gaaatccgat ttgtgtctcc agaatcatca attggaatag caaaaatcca aaaactgatt
    43141 gcgagaagca tcttttcatc aggtatgaaa agaaacttct atcgtgatca gtttttgatt
    43201 tttttaaaat tcatttttct aagactggtt agcagaaaga agagaataat ctcttctctt
    43261 ttttctatta tatatagatt ttctctattt tttggtaagg gagctgtttc aaaaatggta
    43321 aagagcggaa atgttgcgaa tatcagttat ttaatgatat gatattcgga aaaagaaatc
    43381 tcaaagggag gtaactttta tggggttgaa tgaatttatt ttaggattga acaatttaga
    43441 gacgatcaca agggcacctg gattttttaa atatacagag catacagttg cagcacattc
    43501 gtatcgtgta gcatctattg cgcaagttct aggagacatt gaagaagcga atggacaacc
    43561 gatcgattgg aaactgctct atgaaaaatc attgaatcat gactacacag agcgttttat
    43621 tggtgatatc aaaacgccgg tgaaatacgc aagcaaagag ttaagaggga tgctgcaaac
    43681 agttgaagaa aagatgacgg acgaattcat caaccaagag attccagctg agttccaaga
    43741 agtttatcgc cgtcggttgt tcgaaggaaa agatgaaacc gtagaaggag aactattagc
    43801 catttcagat aaggttgacc ttctttatga atcttttgat gaaatcatca aaaataaccc
    43861 ggaaaaagtt tataaggaga tgttcattga ggcagtgact acgattaaaa gctttgaaca
    43921 tcggccttct gttcagtatt tctttaaaga gatatttccg gaactattga accaggaatt
    43981 ttatggaaaa gaggcgtttt tagaaaccgt ctcaacgatt gtaaagaaag aagaaaaata
    44041 aaaatgatat gagatgcagg gggaagtata gttgaaaaag aatatggatg tcgaatgggg
    44101 ctattctgga gacaaaggtc cggaaaattg ggcgagttta tgtgattggt ttgcgagagg
    44161 tgcggaattt ccattgcagt cgcctattca tttgaccaag ggggaagaaa ccaaaataga
    44221 aggagacacg ctttcctttc attatcaaaa gcaacggttt actgacaagg aattcaaaaa
    44281 cacgattcat ttagtacctt ttgaccaact cagttatgta gagttcaaga aagaacgcta
    44341 ttatttgaca gatattcatt tccacttgcc ttctgaacat atcatcaacg gaaaacagga
    44401 agatattgaa tttcattttg ttcatatgaa cagcaaaaaa gagaatctag ttgtgggtgt
    44461 catgtaccag ctaatggtac aggaaggttg gctgtataat caagaaaatg gggaaagttg
    44521 gaacttagaa aaacatgagc attgggtaaa tccggatgtg ttctttccgg agcaaaaaag
    44581 ccatttccat tatattggct cattgacaac tccgcctacc agcgggccaa tccaatggtt
    44641 tgtaatggat catgtaggaa aactgaacca agagtttctt ctccccttcg atggtcagta
    44701 tgctcgaccg aacaaccgac caatccagcc cttgaatgga agagaaattt attatttcga
    44761 ggagtttgag caatgaatat tcagcagatg aaatacgtgg tcgctgtagc aaacaatggt
    44821 agtttcagag aagcggcaaa gaaaatgttt gtctcccagc caagtctttc agctggtatc
    44881 aaagaattag aaaccgagtt agggatcacg ctgtttactc gaacgaatcg aggggcatat
    44941 ttaacagaag aagggcaaga cttcttaaat cgtgccgaga aaatattggt ccagcttgat
    45001 acattagaaa ctcactatct aacgagtgaa aagagagaca atttttcgat cgctgcccag
    45061 cattatgatt tcttaggtcc tttgaccgca cagctactcg aggaattcaa agacgagatc
    45121 aaacaatttc ggatcatgga gacgacaaca gcgagagtga tcgaagaagt caaagagcag
    45181 cacagtgaga tcggcattat ttatatgaat gagcataatc aagctggtat cagaagatat
    45241 ttacaacaag gagagctaag tggccgcagc attggaaaat tcaaaacgca tattttttta
    45301 aggcaaggac atccattagc tgaacacgca gagatcacca aagaagaatt gttatcttat
    45361 cctcaagttc gttttacaca ggatggaaat aatttcccgt atttttatga agaccttatc
    45421 gagatccctg atcaagaaac agtgatttat acgagtgatc gaggtacttt gatgaatatc
    45481 gttcttgaaa ctgatgctta cgcttcagga tctggtattg tgattggagg gattcgcgag
    45541 cacttgcggt taatcccttt ggcagaaggc gaactaaatg aattttatat catttattcg
    45601 gcgaaacgtc cgctcagtga aatcgctcaa agatttatca aagagcttac tcggttactg
    45661 gaccagcaga gctaagaaaa gcagatgaga acgctgttta ttgaacaccg ttgatcaaaa
    45721 aagaggggtt gtgacagaat tttgtcacaa ctcctctttt tgtagcaaac aatttataac
    45781 taattgctgt tgtgggaaag tacgcaaata tgattctaga aacactcatt gattacctgt
    45841 tttgtttcat agctgtaatt ttttcttctt ctggtgtcac gataatcgtc ttttggtttt
    45901 cataaataac ataaccaggc ttggcgccat ttggttttcg gatatgcttt acctgtacaa
    45961 gatcaaccgg cacttgggca gagtatcggt attttgaaaa ataggcagct aactcggcag
    46021 cttctgtaat tgtttcatct gagggcttgt cacttttaat gatgacgtgc gaaccgggta
    46081 tattttttgc atgaagccaa tagtcggttt ttttagctgt cttcattgtc agctggtcat
    46141 tttgaagatt gtttttacca acaagaatga gcgttccgtc agaagaaaag tattgatcag
    46201 gctgagatgg tttcttacgt ttttgttttt ttaagttctt ctttttcagg tatccttctg
    46261 aagtcaattc ttctttaatt gcttcgatat ccattgggcc ggcaatttcc agttgagata
    46321 agacagattc aagatattgg atttcatctt tggcttcttg gatttgttcg ccaatcagct
    46381 taacagcgtt cttcagcttt tggtatcgat ggaaatattt ctgtgcattt tgatttggtg
    46441 tcaatgccgg atctagtgct attttgattg gccgatcttc ttcataataa ttaggtaaaa
    46501 caacttcttt tgcgccgcgt ggtacttgtg tcagaaaagt agtaagtagt tcgccatttc
    46561 tacggtaatt ctcagcgttt tcggattctt tgagggtttg ttcacgtttt ttcaatttat
    46621 ttttatttcg ttttagttca ttttcgattt ttcgaatcag ttctccgcct tgttgttttg
    46681 cccgatcttt ctctgctttt tcgtgataat agctatctaa taattgactg agtgttggat
    46741 aagctgttac agcagatgcc tgttcggaca atacctgata agcaatcggt gtgaaaaaat
    46801 ctttattttc cgtttcgtaa aaagtaggaa taggctgatg gctgatggct gagaaaaatt
    46861 cgtcccatac aaccatcttt tcattaggac gttccagtaa tcttttggtc agctcttgag
    46921 cgatatcgaa accgattccc tgaaactgac gttggattgc ttttggatct agttcagttt
    46981 gggaaagccg ctgaaagatt ttttctttct cggagctaaa tgggttcaag acctcttgtt
    47041 ttggcggagc aacatattct acaccaggaa gcaaagaacg ataagtgttt tgagagcttc
    47101 caatgtgttt gatcgcatcc agtatttttc cagtttctcg attaactaag acgatcgtac
    47161 tatgacgtcc cattaattca acgatcaata tgatattttg tagatcaccc aactcgtttc
    47221 gtttagcaaa gtggaaatgg atcactcgat cattttcgat ttgttcgatg tcttctaaaa
    47281 tagcgccatc caaatatttt cgaagcatca tcacgaagtt aggcggatta tcaggattct
    47341 gataatcgat ttgggtaatc tgtacacgag cataacttgg gtgcgcagat aataataatc
    47401 gatggttttt gccgcgtgaa cgaatcacca gcacgatttc attttcataa ggctgatgga
    47461 ttttcgagat ccggccagat agtaacgttt cacgcaattc gttgaccatc gcgtgggtaa
    47521 atacaccatc aaaggacatg gttcattcct catttctttt cataagtata ttcattataa
    47581 cgaaaaaaag aagaaaaacc aatttggccg aatagtaaag caaaagtcta gaaaaatctt
    47641 ggactaatta cataaaaaac agtgtttcta tcgttgaaat ccaaattttt a
//
LOCUS       DACEUZ010000007        43903 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.007, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000007 DACEUZ010000000
VERSION     DACEUZ010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43903
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.007"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(127..1518)
                     /locus_tag="IXG44_000339"
     CDS             complement(127..1518)
                     /locus_tag="IXG44_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HAQ1657081.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            complement(1731..2117)
                     /locus_tag="IXG44_000340"
     CDS             complement(1731..2117)
                     /locus_tag="IXG44_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HAQ1657082.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            complement(2173..2796)
                     /locus_tag="IXG44_000341"
     CDS             complement(2173..2796)
                     /locus_tag="IXG44_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HAQ1657083.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            complement(2860..3528)
                     /locus_tag="IXG44_000342"
     CDS             complement(2860..3528)
                     /locus_tag="IXG44_000342"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAQ1657084.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            complement(3540..3980)
                     /locus_tag="IXG44_000343"
     CDS             complement(3540..3980)
                     /locus_tag="IXG44_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1657085.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            complement(4039..4710)
                     /locus_tag="IXG44_000344"
     CDS             complement(4039..4710)
                     /locus_tag="IXG44_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ1657086.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            complement(4895..5293)
                     /locus_tag="IXG44_000345"
     CDS             complement(4895..5293)
                     /locus_tag="IXG44_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HAQ1657087.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(5290..6063)
                     /locus_tag="IXG44_000346"
     CDS             complement(5290..6063)
                     /locus_tag="IXG44_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAQ1657088.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(6060..6743)
                     /locus_tag="IXG44_000347"
     CDS             complement(6060..6743)
                     /locus_tag="IXG44_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAQ1657089.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(6910..7770)
                     /locus_tag="IXG44_000348"
     CDS             complement(6910..7770)
                     /locus_tag="IXG44_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1657090.1"
                     /translation="MERPNVKKQALELRSTIGSVLKYTALSILSFISIFPFVWMVLGM
                     TNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVFALLISSMAG
                     YGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINSFLVVILPSV
                     STAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAAAAIITFMSS
                     WNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTAIVFFVLQKQ
                     FVQGMIGSVK"
     gene            7751..8674
                     /locus_tag="IXG44_000349"
     CDS             7751..8674
                     /locus_tag="IXG44_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1657091.1"
                     /translation="MTLGRSIVSFSTFSEESGYVINTDSKDTLRKFVQKFRQEVFPGD
                     FFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSYLT
                     KIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEELSL
                     LKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYEKE
                     INLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEICEK
                     VGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            8671..10407
                     /locus_tag="IXG44_000350"
     CDS             8671..10407
                     /locus_tag="IXG44_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ1657092.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(10470..11306)
                     /gene="pheA"
                     /locus_tag="IXG44_000351"
     CDS             complement(10470..11306)
                     /gene="pheA"
                     /locus_tag="IXG44_000351"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HAQ1657093.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(11303..11812)
                     /locus_tag="IXG44_000352"
     CDS             complement(11303..11812)
                     /locus_tag="IXG44_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HAQ1657094.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(11805..13100)
                     /gene="aroA"
                     /locus_tag="IXG44_000353"
     CDS             complement(11805..13100)
                     /gene="aroA"
                     /locus_tag="IXG44_000353"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAQ1657095.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(13113..14204)
                     /locus_tag="IXG44_000354"
     CDS             complement(13113..14204)
                     /locus_tag="IXG44_000354"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HAQ1657096.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(14221..15387)
                     /gene="aroC"
                     /locus_tag="IXG44_000355"
     CDS             complement(14221..15387)
                     /gene="aroC"
                     /locus_tag="IXG44_000355"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HAQ1657097.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(15389..16453)
                     /gene="aroB"
                     /locus_tag="IXG44_000356"
     CDS             complement(15389..16453)
                     /gene="aroB"
                     /locus_tag="IXG44_000356"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HAQ1657098.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(16486..17508)
                     /gene="aroF"
                     /locus_tag="IXG44_000357"
     CDS             complement(16486..17508)
                     /gene="aroF"
                     /locus_tag="IXG44_000357"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HAQ1657099.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(18167..19141)
                     /locus_tag="IXG44_000358"
     CDS             complement(18167..19141)
                     /locus_tag="IXG44_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HAQ1657100.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(19122..21005)
                     /locus_tag="IXG44_000359"
     CDS             complement(19122..21005)
                     /locus_tag="IXG44_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HAQ1657101.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(21023..23248)
                     /locus_tag="IXG44_000360"
     CDS             complement(21023..23248)
                     /locus_tag="IXG44_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAQ1657102.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            23363..24397
                     /locus_tag="IXG44_000361"
     CDS             23363..24397
                     /locus_tag="IXG44_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1657103.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(24464..25477)
                     /locus_tag="IXG44_000362"
     CDS             complement(24464..25477)
                     /locus_tag="IXG44_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ1657104.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(25555..25743)
                     /locus_tag="IXG44_000363"
     CDS             complement(25555..25743)
                     /locus_tag="IXG44_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HAQ1657105.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            complement(26183..26311)
                     /locus_tag="IXG44_000364"
     CDS             complement(26183..26311)
                     /locus_tag="IXG44_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAQ1657106.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(26314..27687)
                     /gene="rlmD"
                     /locus_tag="IXG44_000365"
     CDS             complement(26314..27687)
                     /gene="rlmD"
                     /locus_tag="IXG44_000365"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAQ1657107.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(27798..28853)
                     /locus_tag="IXG44_000366"
     CDS             complement(27798..28853)
                     /locus_tag="IXG44_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAQ1657108.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(28863..30293)
                     /gene="gatB"
                     /locus_tag="IXG44_000367"
     CDS             complement(28863..30293)
                     /gene="gatB"
                     /locus_tag="IXG44_000367"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HAQ1657109.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(30290..31762)
                     /gene="gatA"
                     /locus_tag="IXG44_000368"
     CDS             complement(30290..31762)
                     /gene="gatA"
                     /locus_tag="IXG44_000368"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HAQ1657110.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(31762..32067)
                     /gene="gatC"
                     /locus_tag="IXG44_000369"
     CDS             complement(31762..32067)
                     /gene="gatC"
                     /locus_tag="IXG44_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HAQ1657111.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVTETEDGFIKVPAIIDNGEAGA"
     gene            complement(32173..34209)
                     /gene="ligA"
                     /locus_tag="IXG44_000370"
     CDS             complement(32173..34209)
                     /gene="ligA"
                     /locus_tag="IXG44_000370"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HAQ1657112.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(34226..36460)
                     /gene="pcrA"
                     /locus_tag="IXG44_000371"
     CDS             complement(34226..36460)
                     /gene="pcrA"
                     /locus_tag="IXG44_000371"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HAQ1657113.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDLEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            36615..38174
                     /locus_tag="IXG44_000372"
     CDS             36615..38174
                     /locus_tag="IXG44_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HAQ1657114.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(38285..40216)
                     /locus_tag="IXG44_000373"
     CDS             complement(38285..40216)
                     /locus_tag="IXG44_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAQ1657115.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(40234..41148)
                     /gene="pfkB"
                     /locus_tag="IXG44_000374"
     CDS             complement(40234..41148)
                     /gene="pfkB"
                     /locus_tag="IXG44_000374"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAQ1657116.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(41145..41897)
                     /locus_tag="IXG44_000375"
     CDS             complement(41145..41897)
                     /locus_tag="IXG44_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ1657117.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(42057..42266)
                     /locus_tag="IXG44_000376"
     CDS             complement(42057..42266)
                     /locus_tag="IXG44_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1657118.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(42277..43605)
                     /locus_tag="IXG44_000377"
     CDS             complement(42277..43605)
                     /locus_tag="IXG44_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1657119.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
BASE COUNT        12176 a         9499 c         7862 g        14366 t
ORIGIN      
        1 ttcacggtgt ttcagaagta gcttctcgga aataagccga aatttcacaa aaatttgaga
       61 aactattttt aggaaacttc ttcttatttc ttaaagctga ccacttctat cacaacctct
      121 ttttgactat gcattcacat cgatcacttt agaatcatca ttgatgacat cgcgatctaa
      181 atctttgatg cgtgcacttg tcacgatacc agctaccatg ctaccgctga cgtttactgc
      241 tgtacgtgcc atatcgatta atggttcaac agagataact aacccaacga ttgctactgg
      301 caagttcatt gcccctaaaa cgatcagtga agcaaatgtt gcgcccccac cgactccagc
      361 tacaccaaaa gaactgattg ttacaattgc cacaagcatc aaaataaatt ccaaactaaa
      421 gacattcact ccagctgctg gtgcgacgat cgttgccaac atagctggat aaacgccagc
      481 acagccgttt tgtccaatag acaaaccaaa acttgctgag aaattagctg ttgcatcatc
      541 tactcctaaa gcttttgtct gagtctcgat attcaatggc aaagctccag cacttgaacg
      601 tgaagtaaac gcaaagctca ataccggaaa ggcttttttg aaatagttga ttggattgac
      661 ttttgcacca atcaaaatca gtgagtggac aatcaaaaca gcaagcaatg ctacgtaaga
      721 agcaacgaca aatttaccta aattgacgat agctgcaaaa tcacttgttg ccaaaacgtt
      781 tgtcatcaaa gcaaatactc cataaggtgt caaacgcaaa acaagcgtca cgatccgcat
      841 aatgatttta tacagactgt cgactaaatc tgaaaagaat gctgcttctt ttggcgcttt
      901 tcttttcacg cctagataag cgattccgac aaatgccgcg aaaataacga ctccaatcgt
      961 acttgttgca cgactacctg ctaaatcagc aaaaacgttt ttagggataa aggataaaat
     1021 ctgctgtgga atcgataagt tttcaactgt ttcctgacgt gttgccagtt cagagattcg
     1081 tgctgtttct gcagctcctt gtgtgaattt agctccttgg agtccgaaca gcattgttga
     1141 accaattccg atcaatgctg caatagctgt tgttccaagt aaagaagtca atacagtcgc
     1201 actaattttc cctagctttt cagaaacttt catttttgtg aatgcaccaa tgatcgaaat
     1261 aaacactaaa ggcataacta gcatttgcaa gaatgataca tatccttgac ctacaatccc
     1321 gatccattcc attgattgag taacgacttt gtcgtctaca ccaaagatca attgaagcag
     1381 accgccaaag acgataccaa ctcctaatgc tgtaaagacg cgtgttgaaa attttgtatg
     1441 gcgcttttgc atacgataga acacatataa taatgccgca aaaactagaa ccactaaaac
     1501 tgtgatgagt gttgtcattt ttctcctcct catattatta atgacaagtc catcgaagga
     1561 agtaaaggtt cccgagaatg attcttcaac catcctcctg aaaaaatttt tttcagtgac
     1621 ttttatattt tagacgattt ttatcgaaat gacattcttt ttgcttacaa ttagtaataa
     1681 aaaaacatac ctgtgatagc tttaatctat cgcaggtatg tttttactgt ctattcttgt
     1741 ttttctgatt gaaaattcaa ttttacccca ggctgatcta aggcgatctc tgtcacctca
     1801 taagacattc ggcgtacgat atcgaataac ggtgctacta gctcgtccac ttcttcttgt
     1861 gtgacatcat tttgaccttc aattttccga ttgaggatat gattgatctg actcacactt
     1921 ccagataaaa catatgtatc aaacactaaa cggaactcta agcgtgctcc tacgatgcta
     1981 tgttctttcg gatagttttc gatcgtttct tttaaaggcg agaagcctac tttcaaatct
     2041 gtttctactt cttggttcgg caatctcata tcataatgaa atgcttctac aacttctttt
     2101 tgtcttctga tttccatata attttatcac tcctttatga aaattataac atatctcctt
     2161 tgaaagggga tactactttt taatgatgct ctgcaaggta aggatttcat tcagatttcc
     2221 taccacataa tctgccggga tctcacccag aaaatgatcg atgtcgtaaa gacaagtagc
     2281 gactcctgca tttttccctg cttcgatatc taaacgacga tcgccgatca tcatcgtgtc
     2341 tgttctttct aaatgaaaag tatcgatcaa ataatttaaa gatgctggat caggtttacg
     2401 aggaaagtcc tgatcgatcc ctactacttc ctcaatcaga tgtgccaatc gatgttcttt
     2461 taacaggccc catgttgatt ctgtcaaacg gtgggtcagg atgaaatgac gaccgccgtt
     2521 gtcttttagc atttccagta cttgtttcgt ttctggaaat ggacgggaaa cttccgtact
     2581 ttctttttca tatcggtgaa ataattcatt aaactctctg gcattcaagt ttgattcatt
     2641 gatcaaataa ctagtagaat actttttcat tttgaagtag atttcttttt tgtccatact
     2701 gataccaaaa tcttttagtg cttgccatgc accatctacc attgcgggat acgtatcaaa
     2761 caatgtacca tcaaagtccc atatataatt tttcatgttc cttttgttcc attcctttgc
     2821 ttcttttttc tatctctcac ttttagataa aggtgttctt taaagtattt ttttgtccag
     2881 aattgtcaaa tcatctgcta atgtatactg gattggttgt gcatttggga tatcgatcgt
     2941 atccatttga tcttcaggaa catcttctaa atattttgtc agtgctcgga tgctattacc
     3001 atgacccaca actagtaaat cttgccgatc ttttagtttt ggtgctagat gatcttgcca
     3061 taagggaatg atccgattca ctgtcatttc cagactttct ccgtatggga tcaaatgcgg
     3121 atccaagtga tcatatctgc ggtcaaatct attttctgcg gttaacggcg gaacttccgt
     3181 atatcctctg cgccatgttt ttacttgatc ttttccgtat atttctttca tcttatcttt
     3241 gtttttaccc acaagatccc cataatgccg ttcatttaga cgccatgttt ttattatagg
     3301 aagccataac tgacccattt cttctagtgc gacttgtccg gtaagaatcg cacgtttcaa
     3361 aacagaggta taaacggcat cgaattgaag tccggcttca agcatttttt gaccggcaac
     3421 tgctgcttgt tcttttcctt tttctgttaa ggcgacatct agccatcctg tccagtaatt
     3481 ttcaaaattt gcttcgcttt cgccatgacg taataatact aatttcattc ttttctccct
     3541 taaacattcg tttcgaattt tataccacat gttttgcatg tcacacgtat tcgtcctttt
     3601 cctttaggtg cacgctgctt ttgatgacaa ttcgggcact caaaaaaagt ataacttttt
     3661 cgatccattt tgacttgctt tttgtttttt atctgatgat atttttcttt gactctatca
     3721 agatatttca aataacgttg attctcattt agtctggggt agattttttt tgataggaaa
     3781 cgaaatatca tccatccgaa aaagacgatg aacaacaatt gtccgattct atagggcaac
     3841 caacgactta gcaaactaaa gacaagcgca gcgatcaata gaccgcgatt caactgatca
     3901 aatttcgcat atcttccctg cataagtttg tgatagttct gctgaaatcg tattagcctt
     3961 tctaaccatg tttgcatcat attatctcct ccatcatcta atatttatta tgacaaaaaa
     4021 atccaaaaag aaaaagcctt aattggcctt ttccattgca acttggtgaa ggacattccg
     4081 atttctggta aagtcaatca caatcacttt ctttttatct tctctgttga ctactcgaaa
     4141 tgtgtccgtc aatggaagaa acagcttctt agatggtagc tgttgcatac gtataaaacg
     4201 atggatcatc gtaatcaatt gaggaaattg aatagacgtg tgaagcagac ctggatattt
     4261 tttcaacagc tgtgtcgttt tgatcagatt ccgtttttta ctgatcactt ttaaaaagct
     4321 ttttagaatc ttttgctgac gtctaaaaat agaaaaagaa ttttcgtcat ctatttgata
     4381 acgcatttgt ttttgcagtt ctgctgcagt cagatggatc ttcccatcgt ttgtatcgat
     4441 cattaaacct tctggaaaca gttcaaccac tgctcgatcc caagattcaa gagaaagctc
     4501 gatataatct tttactggta attccaattc taccttaaaa aagtgcaaaa cagccgacag
     4561 cccttctgct tgatagattt ctcctaatgt tagcccctct agatggaact ctggtgaaaa
     4621 atgcagcaat gttttatttt cctgcaattg aacatataga aatgtagatc gctcaatcag
     4681 agtatctttt aaaacaacta aaaaattcat ttttctcccc cttttttcat catttttaaa
     4741 atagcatatt ttgaaaagaa agtctgatgc ttagagtaga atttcataaa aatttactat
     4801 ttcataagta ttcacgagaa aaatacaaac aaaaaagcgg aaataacgct catccgcctt
     4861 cttgtttgtg tttatttcct cttcaaacgc ttgtctatga ttcagacttt tctctatcgt
     4921 tattttcatc taaaagttct tgcattccga taatttctcg atattgatga atcttagtga
     4981 taccaatgac tttgtctaca tcaatactaa aacaaatacc attacctgga atatcgatgt
     5041 tgatgccttg accaatcgca tccattactt tattcgtcaa actatcgggt actaatgtca
     5101 acacaatatc tttttctggc gaaatgtcca aacctaaaaa tttccctgaa tcatgtacac
     5161 cagaacctct tccatgtaag atcgttccgc ctgctgcacc tgcttctttt gaaaagtcga
     5221 taacatcttc gcccattcct ccgtcaacga tcgtcacaat catgtgaaga ttcaatgcta
     5281 caagatcttt catttgcttt cccccaattt ttgcaatctt tctttttcga ttctgtcttt
     5341 ttcatctttt cgtttataaa tatatcctag tactaaagtg gataatattg gtgtcaatgc
     5401 aatcattgca accacaccaa acccgtcaat gatcggatct cttccttcaa tttgtgcagc
     5461 gattgccacg ctcattgata aaataaatgt cgaacacatc gggcccgttg ctactccacc
     5521 gttatcaaaa gccattgcta aaaagatctt gtccactttt cgtcctaaaa taaatgctag
     5581 cccgtatcct gggatcaaga aataataaag agagaatccg acgagcaaac gccacataga
     5641 caatcctact gctgcgccga tcccaattga aattaccatc agcatgattt tcgatcggat
     5701 tctcccctcg ctcaattctt caacctgttt gatcattaca tgaatcgccg gctcagcaaa
     5761 ccctactaag aaccccatta aaaagccaag gggaaccagt atataattgt aaggcaatcc
     5821 cgcaatagca ctgcctaaat gctgtcctac cggtacataa gccatattga ctccgtgcag
     5881 gaaaaggatc aacccaatag tcgttatcaa gaaacctttc ataatgtcaa agaaacgctt
     5941 tttgcgcata cgaaaactaa tgatattcat taaaacaaat aggattacga taggtgccac
     6001 tgccaaaatg acttccatgg taacgatttc gattccatct atcacttgct gccagaaact
     6061 cattggaaga tcacccccaa aatcatcacg gcaagaatcg gccctaacga agcaataccg
     6121 accattccaa aactgtcatt ttcatttttc ttttgtttga tcatactagt catccctccg
     6181 gcaagggcca agataaatgg aacagttact ggaccagtag tcactccccc cgaatcaaaa
     6241 gcaatcggca taaattcgga actggtgaaa aatgaggcga tcaaaacgcc tatgtagcct
     6301 atagccatca attgataata ggatagcttg aatacgaccc gaagcagcgc aaaggctaga
     6361 aatactccag tgccgacgct gacgatccca atcagcaaca ctctgccaat ctctccgtca
     6421 gatatatcat atacttgttg tcccaaaact tgcacggaag gttcagcaat cgtgatcacg
     6481 atcccgatag caaatcccaa actgaccaaa acggtcaaac ttttcttttt gatcatatat
     6541 ttccctacta gatctccgac ttccatcata gaataatctg ctccaaacaa aaagagcgtc
     6601 attccgatca tcatgattgc cgcaccaacc agaaatgaag cgaggtcttc tccttctaac
     6661 ggtgcaaaaa caaacgttaa aatgacaatc aggacagtca tcggcaatat agcagcaatc
     6721 acttctttaa aattttttag catgtatcct cctcctttac tctttaagtt taccttaaaa
     6781 tgaaagagtt ttcgagcaca aacttaaaac gttatcattt aatttttctc aacataaaat
     6841 aacacacgtc ccaaaagtat ctttttactt ggaaaaatga ctttgaagga acgcgcttat
     6901 tgtgtttttt tattttacag agccgatcat tccttgaacg aattgctttt gcaaaacaaa
     6961 aaatacgatg gcagttggta aagtagcgat gactaatcca gccatgatca ttccatagtc
     7021 tggtgtatag gaagcaccca tagcagataa aacaagcgga accgtccgtt tatcaggaga
     7081 ttgcaaagct actaagggcc ataaataatt gttccagcta ctcatgaatg tgatgattgc
     7141 agccgcggca taggtatttt tagccgtcgg catatacact ctaaaaaaaa tacttagttc
     7201 ttttagaccg tctaatcgag cggcttctac tagatctttt ggaaaagatt taacattttg
     7261 tcgaaagaag aagatcaaaa aagcagtgct aacagatggc aggatgacaa ccaagaaact
     7321 gttgattccc aaaaccgttc cgttcaattg tgaaaacatt cgatataaag ggatcatcaa
     7381 tgccgcaaaa ggaaccatca tcgatagcag tagaatattg aaaattaggt cttttcgttt
     7441 actttgataa atctcaaatc catatcctgc cattgacgaa atcagcaatg caaacacagt
     7501 ggtcaccaaa gcgatcatcg ctgaattcca aagggaacga gtaaaattca gctcattgga
     7561 aaacaaattt tgaaaattga tgatcaagtt actgccaatc tttatttttc ccgatgtgat
     7621 atccacgggt gtattcgtca ttcccaaaac catccagaca aaagggaaaa tggaaataaa
     7681 tgataggatg cttaatgctg tatactttaa tactgaccca attgtcgaac gaagttctaa
     7741 tgcttgtttt ttgacgttgg ggcgttcgat tgtttctttt tctacctttt cggaagaatc
     7801 cggctatgta atcaatactg attccaaaga tacacttagg aagtttgttc aaaagtttcg
     7861 tcaagaagtt tttccaggtg atttttttgt tgtttctgat tcttttgcaa tccaagatat
     7921 caatgaaata ttccaagaga tcagaaattg caccaaagga caacgtttct atcataaggg
     7981 aaagaacctt gcttatcagc atgaacttct tccgttatta accaaccacg agcagcaagc
     8041 aagtagttat ttaacaaaaa tcgttcataa agactacgct ttagctgtca aagaacttga
     8101 gaatttcttc acgacgatat tggaacagcc tattgatcca gcttatttga aacatgaggc
     8161 aatcaatcgt ttctatgcat tactcaacgc tttggctgac gagcatgttg ggaatgaaga
     8221 actttctttg ctcaagatcg cccttccttc tttattagcc gatgctgagt atttcgaaga
     8281 tttttctatt cttgtcaaag aaacacttga gaaactcacc cacttttctg caaatgcaaa
     8341 aaaagacaat ttcgatatct tgactttgat ctacgagttc gtccaggaaa attatgagaa
     8401 agaaatcaat ttacaacttt tatccgaaaa atatcatttt tcatatagct atctctcagc
     8461 catctttact gaaaaatttg gtatcaactt ttcaaaatat ctaaaaaaag tccgtatcag
     8521 taaagccaag ctctttttga cggaaacacg gcttactttg acggaaatct gtgagaaagt
     8581 cggctatacc gaactgggtt atttctccag agtttttaaa gaagagacag gaatgacacc
     8641 ttctcaatac cgtaaaggaa cttttgtaaa atgagaaaaa aaatcaaaga gcatgaactc
     8701 tttgccaaac ttctgttcat tttgttcatc ggtctttttc tgcaagccat cgtcatttct
     8761 ttgtttattt atcatcgatc aagggatgcc tatatccagc tgttcaatca atcgaatgat
     8821 gtggtactaa aaaagattca atctgaattt gaaagtttga atgatacaat cgagaataca
     8881 ttagccgctt ttgattctaa tccagctgtc aaaagctatt tttccaatga tccagcccaa
     8941 catatggaac agcttcagca actaagaaca atccaaaaaa tgaatgactc tctttcaaaa
     9001 atccatccaa tgatcgatta cgatgtattg atctttggag aaaatggaag aacttttgtc
     9061 ggaaacgaca tgctcactgc cgtatctgcc gattcttttt ttcaatctgc gattgctcag
     9121 agagtgaatg aacgtgctgc tgatacacaa atgctttttg cacaccacgg attgactctt
     9181 cgcgataaga aagcacccag cgtcttcttt gtcaaaaaac tgaaaaatac attgaatcac
     9241 gtttacggat atgctgtttt atcgatctca tccaagcaac tagcgaatct ttttcaatct
     9301 gtagtaaatc ctgagatatc gaagatctcc ttcatgaacg aagaacagac gatcatcgca
     9361 tcaaatgaag aaaatacaat tgggagccga tcaatgattt tcaaaaaact aacagtaggt
     9421 gaaacaacga tttcaaatgg acagcagttg acccgtcttc ctctgtaccg ccaggattgt
     9481 acattactca gtcaagtcga tcttcattct ttatctaacc aaatgggcgt gatttttccg
     9541 attattttgt ataatatcgt gacacttgct tttgctggct cattggtatt tatgtatttg
     9601 gatcgacaca caaaatcgat ctaccgcttg attgattcgc taaaacggct agaggaagat
     9661 ggcttaaaag ctactgttcc tgtacaagga agccatgaag tccaattgct agggaatacg
     9721 atcaatcaat tgctaagtaa tgctcagcat cattatgaac agacgatcca agacgaaaag
     9781 aagaaacgtt cgcttgaaat ccaaactatg caagcacaga tacagcccca ttttatttat
     9841 aatacgctga ctagcttgaa atttttgatt tggcagaaaa aaacagatga agcagttcaa
     9901 ggaatcgaca gtttcattac tttactaaga agtacgattg ggaaaaaaga agaagtgatt
     9961 ccagtcaagg aagaaatcaa aagcgtacaa agttacatcg atcttttgag tatgcgatat
    10021 ggcgaaagaa tctcggctaa aataatgatt cctgatgaac tgcagtcatt atctatgccg
    10081 aatatgatcc ttcaaccaat tatcgaaaac gcctatcttc atgcttttca caataaaacg
    10141 caaggattca tcacggtgta cggcaaaaca aaaaatcagc ttcttttatt cgaagtgatt
    10201 gataacggcg atggcttcga tactgaaaat accaaacaaa aaaatgatca tttttcagga
    10261 atcggcatca gccatgtaga tgagcggata cgcctaatgt atggcgatgc atatggctta
    10321 acgatccaat cagtgattgg gacaggaacg actgtaacga tttgtctacc gcttcttcct
    10381 aaaaatcaat cagaatatca aaaataaaga atagccaagc gctgattttt ttattttaag
    10441 ttaaataaat caatcagtat tcttgacttt catctacgcg tctcttctac tgtcagcact
    10501 ggataacagc ccaatatttg actttctcct ccgagcatct tgatttcttg gagcgcattc
    10561 tctactaatg tcattggacg gtccaacaac aaatcgatca cgaaaaaata ttctcccaag
    10621 ctagttttca atggtcggga ttcaatctta cttaagttga tttcccgcca gccaaaagct
    10681 gccagcattt tatgaagcat ccctggccga ttagcaggta gtgtcaagat gacactcatt
    10741 tttactggag ctgactgctg gctggtcatc tttcggtcac cgacgatcca aaaacgtgtc
    10801 tggttcaact cattatcttg gatattctca gctaagatct ccaatccaac atgctgtgct
    10861 gtttctaatg aagcaatcgc tgctgcgtct tctttcggat gttctgctac atacattgct
    10921 gcagcagttg ttgattctgt tgctactaaa ggaacattag gataatgcgt ttctaaaaac
    10981 tgttgtgatt gtgctagtgc ttgtggatgt gaaaggattt ttgtaatctt tgttgctgga
    11041 ttgcctaaaa gctgctgctt gataggaaga acgatttcac ttttaacttc gacttctgga
    11101 tgtttggaaa gaagatcgat tgtgtgatga acagctcctt ccaatgaatt ctctacagga
    11161 acgacagcca gatcgacttg tttgcgaaac aaagcgcgca aacaaacagg gatggaagca
    11221 tacgcagtca attgttctgt aggaaatagc tgacaagccg cctgataagt gaatgaactt
    11281 tctggcccta gatagctgac gttcatatgc ttttgatcct ttctataatg gtatcgatga
    11341 tcttttctgg tgtttgattg gtcgtgttga ttcgatgtgt cgcactttct tcataccaag
    11401 aaagtcgttg tgccaataat gcttttattt cttctcttgt ttttccaaga gctaaaggtc
    11461 gaatagacgt ttcatcttgt tggatacgat caactagaca gtccgcttct gcctctaagt
    11521 aaagaacaat cggctgtgtt tttaaaaaac gccgattttc tgcactttgg acaatccctc
    11581 caccagtcgc gataattttg tcttcatttg acagctttct taaaatatct gactccactt
    11641 ttcgaaagct tttttcaccg taatagtgaa aatattcggc aatcgacaat tggatatgtt
    11701 cctcaatctt tgtatctaaa tctacatact ctttttgtaa acattgagct aaccctttac
    11761 cgattgttgt ttttccagcg cccatgaaac caatcagcaa aatgctagcc atgagcttct
    11821 cctcctgaca caagttctgc taaatcgtca aaaaatgccg gataagagat tgaaacagct
    11881 tcagctcctt ctagttcgca aggtccatca gctatcaaag cagctacttg cagcatcata
    11941 ccgattcgat gatcgcctct gctggacact tttgcgccat gcagaggtgt cggcccatgg
    12001 atgatcagac cgtctgctgt cgcttttatc tttgctccca ttttctgtaa ttcttctgcc
    12061 accgcatcaa ttctgttggt ttcttttact tttaattctt ctgcgtcttt gatgatcgtg
    12121 attccttctg cttgagtagc cactaaagct agaatgggca attcatcaat cagacgcgga
    12181 atgatttctc caccaatcgt tgctttcttc aaatgactcg tcttgacact taaatcagcc
    12241 gattggttct gttcattgtg attcgtctgg gtaatcgctg cgcccatctc ctccaacaca
    12301 tctaagatac cagttcttgt tggattgacc ccgacatttt ttaacaacag ttggctttcc
    12361 ggaacaagaa gtcctgctgc tagaaagaaa gcagctgatg aaatatcacc tggaacagtt
    12421 atctgttgac cagttaattt ttgcggtccg gtcactatga tcgttttatc ttccactgtg
    12481 atccttccgc caaattgacg aatcatttct tccgtatggt tacgggaacg ttctttttca
    12541 acgattacgg atgtgccttc tgcttgtaat gccgcaaaca gcaaagcaga tttgacttgc
    12601 gcactggcta caggcatatt gtatcggata ggtgataaag agcgggttcc tgtcacagtg
    12661 attggtagat actggtcatt ttcttctcct tgcaagtcag cgcccatctc gcgtaaaggt
    12721 tccatcactc gccccattgg acgtttagac aacgaagcat ccccgaacag agtagtcgta
    12781 aaaggctgtc cagccaaaat acccatcaat aaacgtatgg tcgtaccgga gtttcccatg
    12841 tcaattgatt cggttggttg gacaaatgag tggctaccgc gaccatgaat cttgatcacc
    12901 tcttcctctt cttcgatctc gacacctaat tgttggaagg catgtaaagt actcagacaa
    12961 tcttctgcgc gcaaaaaatt ttgtatagtc gtcgttcctt cagcaattgc tccaaacatg
    13021 atgctgcgat gggaaattga tttatctgct ggtatcctaa cagtccctct taatccatgt
    13081 atttgttgta ataattgcat ttatccagcc tcctatttga tccagcaatg ataactggta
    13141 ttagacataa tgattctttt tgcttgtaac aaatcctgtt ggtttttaaa actgatttgc
    13201 agtattccta aaatatcttc cctggtctct tggatcttca aattaatcaa agaaactccc
    13261 gcttttccga tcagtccagt tacttctgca ataacgccag gttcatctgg cacatcgata
    13321 aataaatcaa aaaaggcagg aatcgcgcct tgcttatgga caggcaactg gtctcgactt
    13381 tcttttgccc gattgaaaaa agaaaagatt tccgattggt tctcagtcat gatccattct
    13441 gctatttgtg tcatttcttg ttgccaatct gccaatcgtt ccaataatat ctgcttattg
    13501 ctcaacaaaa tatctgtcca catgacagga tctgaggaag cgatcctagt gatatctcga
    13561 aagcctcctg ctgccagttg tttggctcgc ggatactctt gattgaattg ttctgcctga
    13621 ttgactaagc cagctgccag aatatgtgga aaatgactta gcattcctgt tatacggtca
    13681 tgttctcttg ctgtaagagt tacatatttt gctttggttc cgcaaaatag ttcttgcagt
    13741 ttttcaacat cttttttcgt cttttccaaa ggtgtaaaga tgtaataggc attttcaaat
    13801 agtttagcat ctgctgcctg tatacctgat ttatgagaac cagccattgg atgtccaccg
    13861 acaaaccgaa gatttttctg ctcagcggat gtcacgactt gctgcttcgt actgctgaca
    13921 tccgtaatga tgacgtgatc agacaagttg attctttcaa ggacatcaag ataagctaag
    13981 gttgtacgga cgggaacggc taataagata acgtctgctt gttctgcacc tgtttcaaaa
    14041 tcacttgcaa cgtaatcaac gatctgctgt tctactgctt gctcacgggt ttttgactga
    14101 ctatcccaac caactactat cagttctgga ttggtctgac gaatacaaag tgccatagaa
    14161 gcaccaatca gtccaagccc cacgataaaa acatgttgtg ccatcttatt tgcccccttt
    14221 ttagtaatgt ttcaagtagt cacggtactc cttcactgca gctttcaact caacaagcga
    14281 atcacttggg aattttgaca atagctctgt cgctaccaca gtggctacaa cattttccgc
    14341 aacgacgcta gctgcaggaa ctgctgtact atccgaacgt tctacacttg ctttgtaagg
    14401 ttcttttgta tcgatatcaa cactttgaag tggcttgtac aatgtaggaa tgggtttcat
    14461 gaccccgcga atgacaagtg gttcgccatt ggtcattccg ccttcgaagc cgcctagatt
    14521 attggtacgt cggaaatagc ctctttcttc ttcccatagg atctcatcca tcacttctga
    14581 gcctggtaat gaaccagaag tgaatccgac cccaaattct acgcctttaa aggcattgat
    14641 actaaccaca gcttgtgcga ttttggcatc tagcttcgtg tcccattgta cgtagcttcc
    14701 taatccagct ggaacgcctc caacgagcac ttctacaatt cctccgatcg tatctccatt
    14761 tttctttgtc tgatcgatca aatcttttat tctttgctca actgctggat caacaactcg
    14821 tacttctgac gcttcagaaa tttctttgat ttcttgaatc gagatatttc caggtatttc
    14881 tgcttggatt ccaccaagac aaatcacgtg tcctgctacg atgatgtcaa gttcttccag
    14941 aattttcttg gcaattgcgc cgattgctac acgcatggtc gtctctcttg cagaggaccg
    15001 ttcaagcaca ttccgaagat ctcgatgatg gtatttcata ccgcctacca aatccgcgtg
    15061 tcctggacgc ggacgtgcaa ctcgccgcag tgattggtct ttttcaggca cttctgctac
    15121 agacatgaca gttgtccaat ttttccaatc tttattttct accactaaag tgacaggtga
    15181 accaattgtt ttgccgtgac ggatgccaga cgtgatccga acacgatctt tttcgatctt
    15241 catccttcct cctctgccgt aaccagcttg tctgcgagcg agttcgtgat tgatttcttc
    15301 ttctgacaga ggtaatcctg ctggtagtcc ttcaataatg gctgttaatt ctggtccatg
    15361 ggattctccc gcagtcatat aacgcatctt acagtcctcc taagtaatct ttgaatgtct
    15421 ctgtgggaac aggaacgatt tttgcttgcc caatcttttc tagcaagatg atattcaatt
    15481 ggcttcctct tgctttctta tcatgggtta tcgcgtggaa taattcatct ggatgactta
    15541 gatcgtacgt aatcggcaga tggaattttt gaagtatcct ttctaaagca gctgtcgatc
    15601 cagttggaga caagccgatt ttttctgcat gagaagtgat ggctaccatt ccaatagcta
    15661 ctccttctcc atgactgact accccatatc ctgctgtatt ttcaattgca tggccaattg
    15721 tatgaccaaa atttaaaata agtcgatttc cttggtcaaa gacatcttct tccactactt
    15781 ttcgtttgat ttccaacgta gcagtaatga ttggaacagc atgttccaga agatcttttt
    15841 cgtctttcag ctcgttcagc tgtttccaaa gcgttgcatc tgctatagct gctgatttga
    15901 tgatttcagc gattccttct cgtaaacgac gtaccggcaa agtttttaga acctcaggat
    15961 caatcaatac accagctggc tggctaaatg ttccgacaag gtttttcgct ttgtttgtat
    16021 tgactgctgt cttcccgcca atactagaat ctacttgtgc aagtagcgta gtcggaattt
    16081 gaacgaaatg gatcccacgc atgaacgtcg aggccacgaa tgctgctaga tcacccacca
    16141 ttcctccacc aagtgcaagt atcccatcgc tgcgggtaaa atcattatct gctaaaaaat
    16201 cgtataactc agctgcttgt tctaaagatt tactggtctc acccgccggt acgattgaaa
    16261 ggactggttc aaaattagct tgtctcaatt ggaacatgat tttttctgca tatagtggag
    16321 caacgtttgt atcagtgatc acagctactt tttgaggtct ccaaatcgtt tgaagccatg
    16381 atccagcctg ttctagtgcg cctttttcaa tatgaatatc gtatttttgg ttatcaacat
    16441 gtacagtaag cattcgtttc acttctttct tttaaaaagc tggaattaat cgagtgtctg
    16501 aatcttttcc attgcttctt tcatgatcgc aacttctttc atcatgcgtg agtaagtctt
    16561 ttcattcaac gattggggac cgtctgacca agcatttgca ggatcaggat ggatctctac
    16621 gatcatgcca tctgctccgc ttgccacacc cgcacgagcc attggtggta caagttccca
    16681 aattcctgta ccatgacttg gatcaacaat cacagggaaa tgagttaatt ttttcatcaa
    16741 tgggacagca cttaaatcaa acgtattacg tgtcgctgtt tcgtaagtcc gaatcccacg
    16801 ttcgataaaa atgacaggag atttgtcttc gactgcaatg tattctgcag cattaagcca
    16861 ttcattgatc gtaccggaaa ttccacgttt caatccaaca ggtttacctg ttttaccgac
    16921 agcagatagc agtttaaaat tttgcatatt acgtgcgcca atctgtagga tatcgctgta
    16981 ctcagccacc atatcgatat gcccttcatc catcacttca gtaatcactt tcattccaaa
    17041 ttcatcagcc gcttgacgga tgtatttcaa tccttcttct tctaaacctt ggaaagcata
    17101 tggagaagta cgtggtttga aggcaccacc tcgcagaatt tttgcgcccc cggctttggc
    17161 cattcgcgca cattctctga tttggtctaa tccttcgata gaacaaggac cagccatcgt
    17221 cacgaaactg ccgtcaccaa tttttactcc gtctacatcc acgactgtat tgccaggatg
    17281 gaattctcga gaagtcaatt tgtatgtgtt taaaatacgc acagcatttt ctactccttc
    17341 atagcgtgta aatgctcctt cttgtaaact tcgcgtatca ccttttagtc caatcactac
    17401 tcgttctgtt ccttgatcga ttgagacttt gaatccttct ttttcgatat gatcgacgac
    17461 tttttttatt tgttcttccg ttgcttcttt tttcataatt actatcattg tttttcccgc
    17521 tttcttttgt ttacttatct atcttggctg attttctcta ctttttcaag gtggttttac
    17581 attaattcct actaaatgac cgtgtcctgt tgttctcatt ttcgctcctt ctttcctact
    17641 tatgacccag ataatcaaca aaaaaagatg tccctgtagg aaattgcctc tacagtgaac
    17701 atcttcaacg agccttaaaa agcgctgcaa aaatataaaa tagccactat agatgaattt
    17761 ctctacgtgg ctgtaagtcg tgtttaaaaa tctttagcca tcatagatac aaacctggtt
    17821 tgtttgtacc cacgaagtca tgaatacaaa cctatctaaa atagctaaag taatacgtat
    17881 tcagttttgt aagttttcta gatttcataa aaaatcgctc caaactgatg taagaattgt
    17941 acctagtata gacataaaaa aatcgactgt caatcatttt tatgaaactt tttttaattt
    18001 tctgatagtt aactttgttt ttcagaaaaa taacaattgg ttttattgaa gaaaatagtc
    18061 atgaacaaaa gtatctgaga acttttttac tctcttgttc atgacatata gaataaagca
    18121 gctttgtctt gtaaactccg tttttcaaat aataatctaa agattgctac tgaataataa
    18181 agccgatttc atggtcctct tcgctgctga ttcggtaagg tgactcaaca tccgctcccc
    18241 agctattgat acccccgact cctcttactt ttgcaaaaat cgtaagaacc gttcgtcttt
    18301 ctggcggtag ttcttctata tgtgttgcct gttccagttc ttctgctttg aatggtaaag
    18361 cggaaaaagc aaatggcgta tcctgctgtt caaatcgcaa cgaaaaatct tcttgatctt
    18421 tatccgtatt atccaacgtt tgatttcttg ttagttctac ccatttcgtt tccatatgca
    18481 tgccgcattc ttggggaaca aggtatgggg aaacggggag tcctgccact ttataaatac
    18541 cttcgacacc accagccatc cgatccggat atgtttcccc tgataggcct tcatactcaa
    18601 aaaattttgc tttcgttggc ataatgaacc ttaaacccaa tgcgggtaat tccggtaatc
    18661 ctttttgtcc ataataatgc atattgacaa gcattttacc ctctccagcg atttggtagg
    18721 aaattgtcac ttgggttttt ggtactgtgt tgatttccag tgtaaaaatg atttcaaccg
    18781 tttcagcgta ttcattactt gaatattgat tatttactgg agcaatcggc aaatcgatct
    18841 ttttattgtc caccagtact tctatttctt tgacttttgg atacaatccg gctcctaacc
    18901 acataccgga tttaaatcca aatccgctac ctctgtcatt atcggtcgtt gctcgccaaa
    18961 aaactggaag tgtttcccgg tatagccatt cttttccgtt tttattgagc gattccagtc
    19021 cgcctcttgt ataagaaaaa atataatgcg tattccccga tttgacacct aatgtacagt
    19081 cgccgaagac gattgcaact ttttccgtat ccatatgact tttatttact gtatttttca
    19141 taataatatc ttacctcctg catggcgggt ttttcttgtc catctgcaaa aacgatccca
    19201 ttccctgaga attcatagtc acttggttta tcgtcaaagt caccgccata gcgcatcact
    19261 tctctgcctg tgattggatc agtcacttgg atcgcctgat ctttaaaatc ccatatatag
    19321 cctccttggt acatttcata acgatcaagc aacgtcatgt aagaatccat tccacctaac
    19381 gaattcccca tgtcatgcat atattcacat aagatgaacg gtttttccgc tttattttca
    19441 aggtagtcaa tgatctcttc tggtggcgca tacatgcagc tttttacatc tgaaatcgaa
    19501 tcttcgaatg ctggatttgg aaaattccct tcataatgga caagtcgatc ggggtctact
    19561 tttttgaagt aatcgttcat ttgtcgaatg ttttcttccg cataagattc atttccaaga
    19621 gaccaaaaaa gaatacttgg atggtttttt aatgtttcga aattcgtttt tgctcgatca
    19681 agaacagccg cctgccactg aggtactgca cctggtacgt tgtaagaagg ctcaaatgcg
    19741 cccatttttt gccatgagcc atgggattcg aggttcgttt ccgccatcac atggattcct
    19801 gcttggtcgc ataaataata ccaagtgatc tggttggggt aatgagatgt ccgtacgccg
    19861 ttgatctttg cattttttat gatagccaaa tctttttcca tatcttctat agtgatggcg
    19921 cgtccttttt tcggatgcca ctcatgtcga tttaccccgt tgataatcat ccgttttcca
    19981 ttgaagtaga tgattttatc tttgatttcg attttacgaa aaccgaagga atagtcaata
    20041 tactccgtta ctcttttttc ttcgtctatc gtctccaatt caagcttata taaattgggc
    20101 tgaaaatgcc cccacagtgc cacattttca aatccatcga tgcgaatcgt catttgctct
    20161 tttaacggat attgctgttg atagatttcc ttcccatcag gatctagtat cgctacattc
    20221 agctgactgt ttttggctac ttgctcaaaa gacaaatcta cttcaagttt gccattttga
    20281 catttgtcat ctaagcgagg tttgatccaa agatcttcta aatggttttg tcctcgagca
    20341 actaagaaga catctctgaa gatcccaaaa aatcggaaaa agtcctgatc ttccaagaat
    20401 gcagctgtac ttcttttgta aacctccaca gctagaatat tttcttcttc caaataaggt
    20461 gttaaatcaa attcagacgg agtgaacgaa tcttcggcat acccgataaa atgaccattc
    20521 aaccaaataa acattgcttg ttctacacca gcaaaatgaa tcgagatttg ttttcccttt
    20581 agaccttctt ctaatcgaaa agtcgtcaaa tacgaaccaa ccggattgtc tttagcagct
    20641 gaaaagatcc cttccgcctc tttctcaggg cttgtcgtga aagggggacg gcgaaataca
    20701 tgtccctccc acggatacat cgtattgatg taatggatct tgtcataatt tgctaattca
    20761 atatgactag gaacagtgat ttcatcaaaa gaacaggcat caaaatcttt tttataaaat
    20821 gtttttggtc gtaccgaagg atttggcgaa taacaaaaac gccatgatcc atttaaagac
    20881 tgtttaaggg aggatgtggt ttctacttct tcccttgttg cataacaaac atgatcgcta
    20941 tgtgcatttg attggttcac tctaaaaact tgtggatcat ctaaccattt tatatttgct
    21001 ttcatacttt tttccttctc tctcataact ttttgaaatg aagcagttct gtttggaaat
    21061 caccttgaaa atcggggaag taaagtccaa ctgtcatcaa ttcggccccc gaaaatatcc
    21121 gcttcgaggt ctgttcctga taaagtgcct catcatctaa ccccatcaga tatacttcat
    21181 ggaacgctgg ttgagctgaa gctagtatcc tccctaaaaa cacaatagct tcatcttgtt
    21241 taggagacac aaacatccat gcggcaatat tttcttcaaa aggactcgcc aaacgataat
    21301 aatggccata ctggatcacc ggacgaatcg tttgatagga tataatttgg tcttttactt
    21361 gctttttttc ttcaggagac agctttgtca aatccagttc ataaccaaaa acagcgctca
    21421 tagctgtttc tcctcttgtt ttcaacgaag tagatctgtc ggtctgatga tttggtacag
    21481 ctgatacatg agctgtgatg gacgaaatcg gataagcaag gcttgttcca tattggattt
    21541 tcatacgctc cactgcatcc gtgttatcac ttgtccatga ctgcggcata taataaataa
    21601 acccggcatc aaaccgtcca cctccaccag aacagccttc ccataagatt tccggataat
    21661 cagttgtcaa atgttcaagt agttcataaa gcccgagcat ataacgatgc gctacttctc
    21721 cttgtctctg tgggtcaagg gtaatcgaat agacatctga aagcgaacgg ttcatatccc
    21781 atttgatgta gtcaagagga atcgtatcta aaatctttct catttgtttc tcaatcgtct
    21841 gccgaacatc taaccttgta aaatccagta catgttgtga tctggaagca gaaggcattc
    21901 tacctggctc ctgcataaga aaatcaggat gtgttcgata cagttcagaa tcaaccgaga
    21961 tcatttcagg ttcaaaccat aaaccaaatt tcaatccttt gctatgagca taatctgcta
    22021 tttcacgtaa tccatttgtc aacttgcctt catactcgaa ccagtctcct aatgaagaat
    22081 tgtccgcatc tctttttcca aaccagccat catctaatac gaacatttcg atgcctagct
    22141 cctttgcttc atcaatgatc gccttgattt tttcactatt gaagtcaaag taagtggctt
    22201 cccagttatt gaccagaatc ggccgttcag caaattgatg acgacctctt gcgattcttt
    22261 cacgtagcaa ctcatggtgg atctgactca ttttgttcaa tccctgactt gagtaagaaa
    22321 ggatcatttc aggtgtttga aaagactggc ctgcattcaa ttcccacgaa aaacgatggc
    22381 tgttgatccc accgacgact ctcaattgat tgatctgatc tttttcaagt tcgaaagaat
    22441 ggttgccgga ataaacaaat tgaagtccga tcgcttctcc agtattttct gtcgtatgat
    22501 gatgaactaa agcaataaag ttattcatat ggtgactgct cgttcctcga cggctttcaa
    22561 atacttttgt tccagataaa acagattgcc gtgaaatctg tctttcattg acatgtgctc
    22621 ctggcaaagc aatgacttca tcaaatcgcc tagtatgggc aaagtctagc tggaaactag
    22681 ccgctttttc tagtttgatt gtttctccgg tttcatttct tatctttaca gatcgtgtga
    22741 tcactgctct atcagtaaat atggtataga acaaatcgaa ataaatcgct gtttctctat
    22801 ccttaagtga aatcaccaac gtttgtgcct ctgacttgtc catcacataa gtttgaggca
    22861 agccttcgat ctctggcttc ccatctgtaa tataatggct gtcatatctc agatccagta
    22921 agtctgatcc attcttgcgc tgaatgatcg atgcgggcgt tcgaaagtcc atgctgccat
    22981 gagaagagta ttcttgcggt agtgtatctt tcgacagaga ccggtcagga tttaagggaa
    23041 catttcctga aaatccacga tctcttcggg gataattttt attgtctttg tactgcttga
    23101 ctggtttgcc aaaataaata tgcgccaata cattgctttc ttctatcttt atcagataag
    23161 aaagaaatgt atttcgtaaa tggaagactc ggctcttttc atcaaataag atgaaatttt
    23221 tatctgtttt tctatgcgta atatccatca tttcactccc aacaaaacgg aaaccgttta
    23281 ctttttttta taattcctgt atactgttta ctaaacaatt ttgtcaataa aaatttttta
    23341 tttttttgag gagtgtgtaa aaatggttgg aattagagat gtcgcacgaa aggcaaatgt
    23401 ctctcctgct acagtttccc gtgtgttgaa taacgatcag acgatccatg tggctcagac
    23461 tactcggcaa cggatagtag aggcagctgc tgcgttggat tatgacatca gtactcgtaa
    23521 atatatcaaa aagagaatgc cttcgattgg tgtgatctct acgatcggca aagcgagcga
    23581 agaacaggat atctattaca aacatttgcg agtagggatg gaagaagaag ctcgacgtct
    23641 gcatttaggg atgaatcgaa tctataatct atctgataat cctaaacaat gggaagatct
    23701 agatcagtta ggtgcggtga tcgttgttgg gactgttacg aaagaatcga ttaccgcctt
    23761 gttaaaacaa aacatgcaaa tagtcgtcgt ggataaccct gacatccagc aagaggtcga
    23821 tatggtgtat gcagatcttg aacggatgac taaaaccgta ctggaattgt tcttagaaaa
    23881 tggtcataaa agaatagcct atatcggggg atataaagtc gatatggatg aattcggtgg
    23941 gaagcagatt tctataaatg aaaagaggat gcgtgcctat cagcatttta tgatcgatca
    24001 tcacttagaa aaatatatgt cttatcatct aggtggctgg acagaaaaac aaggtgaggt
    24061 acttgcgact aatctattag aaaatgaatc ccctcttcct actgccattc ttgtcgggag
    24121 cgatccaatg gcaattggtg tctatcgggc attgaaacaa aaaaatatcc aaattggaga
    24181 agagattgtt gtagcaagct ttgataatat cgagacaatc gcaaacctct cccctggctt
    24241 gaccagtgtt caaatcaatg cgaaatcaat tgggcaagca gctgttcgaa tggcattgga
    24301 gcgaattgac ggtatacggg acgaaccaat cattttgact tatcctgcac gattgattgt
    24361 tagggaaagt tttatcccaa gaacaaaaag gtattgaaag agaggttgtg aaaagccgct
    24421 tcgtttcttc atactcaaaa cgaaacggct ttttcacaac ctcttactta tacttattca
    24481 caaaatccaa tgtctgctgg taacaatttt gcgattcttt tagcgtgtaa tcaaattggt
    24541 attcatgggt aatctctttt tttgtatctg tataaaacaa ggattgcacg ggtaccttta
    24601 attctgtcaa ccgattttct aatgcttgac cttgctcctg gaatgagtat gcatttccgt
    24661 cagttatata cgttggcgga aattccttag aaacatggtc agccacacta gcttctttca
    24721 attgctcact cgttttccaa tcttttgttc caatcaacga ccacgcaacg gttttgacga
    24781 aaaacttcat aaatcgatca tccgaatgct gctgtgctgt tagcttcaaa tcaacaggtc
    24841 cgcaataaga aatggttcct ttcagactat cttttggaag tagctgtttg attttcattt
    24901 gtttagcata cgcatcattc gtttgtatag ctgcatattg caatgcgatt tgtgaacctg
    24961 cactatctcc accaaaaaat aattgttcca tgtctagcaa tttgtcgtct ttttcttcag
    25021 ctaataaatc tttgatcagt tcattcactt gaagaacttg attagggtat tctgaatcag
    25081 gcgctaattc ataattcatc gcaaccactg cgatgttggc gtctgaaact aattttgtcg
    25141 caaattcttt gacacccgat ttatctccgc cgacgaatcc tccaccatgt acccaaaata
    25201 gaacaggaac aggccccttg ctgttttttg gatagtagat gtcataagta ttctctttat
    25261 acttggactc atacgtcaaa tctgtttttt tcgccacgtt tttttctgct tgatcgaacg
    25321 tttttttatc agtgatttga acagtatttg aaaacatatg gttgatcaag aatgctccag
    25381 gtctggggga aagctggaag gcaagaaaga ttgcaaacgc gatcccaaga atgattccga
    25441 agaaccatcc gataaattta agaattttct ttttcataag gctccctcag tagttagtat
    25501 taagcattct gattatacca tagtgttatt tcctatcaag ctctcctaga tgatttaatg
    25561 attttcccca aacttcttct gcaatgcttt tgacaaaaga aattttttcc attgttcttc
    25621 aaacaaaaca ttttcttctt ctgttgaagc aaatccacat tgaggactta agcacaaacg
    25681 atccaaaggc agatatcgtg tcgcttcgta gatccggtca atcacttgct ggtgctcttc
    25741 catttcgcca tttttcgtta cagtcaaaat ctaagacaac taatttatct tcagagactt
    25801 tctctaaaag aacaaaactc ccagcacgtt cactatcgta ttctaaataa taggcacgga
    25861 cattttcttg agtaaaataa tgggtctgct acgtcgtcat cccccctgat gccgccaaaa
    25921 tcgaataata atttccgcgt caaacatgta tatttagtgt tgaatcattt gagaatatga
    25981 aaataaaaat ctttttgatc aatcagttaa tcaaattgaa ataacgaaaa atctgagaca
    26041 gaatgacgtt cattctgacc cagatttgat tttgcacaac tcaacagtcc ttattggcaa
    26101 atcgccaaaa taatgtagac taactaaaaa gactaagaaa caataacaaa agttaacact
    26161 tcttagtctt tctttagcac gtctatttat taaagatttt agggcctttt ctcatgtttt
    26221 tacgatcatt cttaggtgtt tccatttgtt tggatcggcc tccaccttgt ttctttttga
    26281 tcaattcttt cattttttct ttttggtcca tttttattct cctttaacga atgaagcgac
    26341 taattcgata tgatgggttt gcgggaaaag atctacaggc tgaactttct tcacggcata
    26401 acctgcttct gcaaataact tagcatctct tgcaaaagtt gccggattac atgagatata
    26461 gacgattcgt tctggtgaca tttcgattgc tgattcaata aaatgatgat ctaatccttt
    26521 tcgtggaggg tcaactacga tcacagatgg tttgatccct ttttcttgcc attttttcat
    26581 aactttttct gcttttccga cttcataatg aacattttcg attttattat ttaaagcatt
    26641 gaactgtgca tcttcaattg cttctggaat aacttccatc ccatacacct tatccactct
    26701 gtctgctaaa gacaagccaa tcgtacctat tcctgaataa gcgtcgatca caatgtcttt
    26761 tttatctaac tcggcaaact cgatggctgt ttggtacaga tactctgctt gttgagtatt
    26821 tacttgataa aatgactttg ctgagattcg ataggttttt cctaatagct gatcttcgat
    26881 atagtctctg ccatataaaa ctttttcttt gtctcctaaa atcacatttg ttttttcttc
    26941 attgatattt tggatgaccg aaaccacttc tggcaattct tcatgaatag cttcagcaat
    27001 ttccttggat ttaaagaatt tttctttacg agtaactaaa acgaccatca tttcatggga
    27061 ataatgacca cgacggataa tgatgtgacg aagctgtcct tcattttttt cttcattata
    27121 tcctcgtact tggaaccgtt gcaagatatc tctgactact ttgattgctt gatcgatgac
    27181 aggatcttgg atataaaaat cttcgatagg gactagatca tggctattct ttttgtaaaa
    27241 accggtggct aactgtccat cgatacggcg aacagggatc tgtgctttat tacggtagcc
    27301 gaatggttct gccattccga ccgttggaag tacaggaact tctggcatct tagcaatctt
    27361 cgccattaca tgctctactt gtttttgttt gaagacaagc tgttgttcat acttcaaatg
    27421 ggctaaaggt gcgataccta tgcgaagtaa tgcattgttt tctacaggct gacgatcagg
    27481 tgattgtgta ataaatgttt cgacttttcc atatccaaac ttattgcctg cttttactac
    27541 tagtatttct acttcttctc cagggagtgc attttcgata aataatggat aaccatcgat
    27601 ttttgctacc cccattcctt cataactcaa gtcgacgatt tcgactgtat atcgttcgtt
    27661 tttctttact atttgctgtt ctgtcatatt ttgctccttt aagcctattt ttcaatccta
    27721 ctgaactcta ctataaatca gctaattaca caactaaaac cggcactgac cttactacgt
    27781 tcagcaccgg cttttgatta attcttttga ttaggtgttt cgtctgctat ttttccgtca
    27841 ccgtcgatat cttcatccgt taaacgttcg acttctttta cgaattcttt gctgacaacc
    27901 tctaattctt cttcatctgt acctgtgatt gcttcatcag gaatctgatc ggtattggca
    27961 aagaactcaa tgtgttgatg aagattttgg aaatgcatcg gtgcttcccc gccgtattca
    28021 ccatctaagt tgatcattac tggtcgattg atatcatctt ctgtttcaac gaacaaactg
    28081 cttgttttgg tatagattat tcttggatca tcgacatgtc gaccgccatt cagcatgagt
    28141 gctgcaatat gcaagatctc aaagatattt gctgtcttca caatgattaa agaaaacttc
    28201 ccatcatcca atttggcatc tggcgcgatc tgttcgaatc ctccaacaga gttcgtcagc
    28261 cctaggaaaa acatcgaagc atttccgata tattcccctt catcatactt caagcgcatt
    28321 ttgattggct ttactcgagg gagcatctct gctcctttcg caagataagc aagataacca
    28381 aaaatactct ttagttctga tggaacttca taagtcaatt ctgtcaaata gccacctgca
    28441 gcgatattga tgaagtaact gttatcagaa aggccgatgt ccatttttac ggtttggtgt
    28501 ttttttatca cttctgctgc tgctttaata ttatctctag gaatttttaa tgctcgagca
    28561 tagtcgttcg ttgtaccagc tgggatgatg gccattttag gacggtgttc aagcggagcg
    28621 attccattaa ccacttcgtt gattgtgcca tcgccaccag ctgccactac tagatcaaaa
    28681 cctagttctg cgactctaca tgcttcattc ttggctgaat ctggttctgg tgtagttgca
    28741 taggcgctgg cttcaaatcc tgcttcttct aatgcctgta ggatatccgc caaatttttt
    28801 ttcaataatt cttttcccga ggtggggtta taaattacgc gtgctttttt cattttcagc
    28861 ctctatcgtt tcgctaattc atctttaagt agcttattca cgacaccagg atttgcttgt
    28921 cctttagttg ctttcatgat ttgaccgact aggaagccaa ccgcacgatc ttttccattt
    28981 ttgaaatcat cgacagattg ttggttgtta tccaaaattt cattaatgat tggcagtagt
    29041 ttgttcggat cagataattg cacccaacca tttttttcaa ctacttcttg ggcttctccg
    29101 ccatttgtga tcaattcacg gaagactttt ttggcgattt ttgagctgat cgttccatcg
    29161 gcgatcaaac gaatcattcc tgctaagttc tcaggtgtca attttgtctc agctaattcc
    29221 agtttctcac tgttcaagta agcagaaact tcacccatca accagttaga tgcttgcttg
    29281 gcatctgcac cattttccaa aacagcttca aaaaaatcag acatttcttt tgtaagtgtc
    29341 aataccattg cgtcatactc tggaagtcca aactcacgga tatagcgttc gcgacgagaa
    29401 gctggcatct caggcaaact agcttttact ttttgaatcc attcgtcatc gatcacgaat
    29461 ttcgggatat caggctctgg gaagtaacga taatcactag acccttcttt gacacgcatc
    29521 aaaactgttt tattcgttgt ttcgtcaaaa cggcgtgttt cttgttggat ttctcctcct
    29581 gaaagaagaa cttttgcttg acgtttttct tcaaaagcta aacctttttt cacgaaagat
    29641 aaagagttca agtttttcaa ttctgctttc gtaccgaact cttcttgtcc ataagggcga
    29701 agagaaatat tggcatcaca gcgcatagag ccttcttcca ttttcacatc gctgacttct
    29761 gtaaattgga taattgatcg caatgcttct agataagcgt atgcttcttc tggtgaacgc
    29821 atatcagctt cagaaacgat ttcgatcaaa ggcgtgcctt gtcggttcaa atcaacgtaa
    29881 gaataaccgc caataccatg catgtttttt ccagcatctt cttctaaatg gacacgttcg
    29941 atccggattt ttttcttttt accttctact tcgatttcga tccatccgtc atgaccgatt
    30001 ggttgatcaa attgggaaat ttgatatgct tttggattat ctgggtaaaa gtaatttttc
    30061 cggtcaaaat gagtatcttt agagatttca cagtttaatg ccaaagctgc tttcatcccg
    30121 aattccagcg cagctttatt cattactggc agcacgcctg gataacccca gtcgatcacg
    30181 tttgtattgc tgtttggttc tgcaccaaaa tgagctggcg ctggagagaa aatttttgag
    30241 ttggttttta actcgacgtg gacttctagt ccaatgactg tttcaaaatt cattcgtctt
    30301 tccctcctaa aatagctggt tgttgtttat ggaattctgt tgcttgttca aacgcatatg
    30361 ccgcttgata catcgttttt tcatcaaaat aattaccgat gatctgcaat ccaactggta
    30421 atccttctga taaacctgcc ggtacagaca tacctggaag tccagccaag ttgacaggga
    30481 tcgttaagat gtcactcatg tacattgtga ttggatcgtt aatgttctca ccgattccga
    30541 atgcaactgt tggtgaagat ggcccgatga tcaagtcata atctgcaaag actttttcaa
    30601 aatctcgttt gatcaatgta cggacttgtc ctgctttttt gaagtaagcg tcataataac
    30661 ccgcgcttaa ggagaatgta cctaacatga tacggcgttt tacttcttca ccgaatcctt
    30721 ctgaacgtga gttgacatat acatcatcta atgatttcac attttcagag cgataaccgt
    30781 agcggatacc atcgaatcgt tgcaaattgg aacttgcttc tgatgaggca atgatataat
    30841 aaacagccac accgtatttt gaatgaggca agctgacttc ttccacaaca gcacctaatg
    30901 aacggaatgt ttctgctgct ttcaatacag cttcttttac gccaggggcc acaccttcgc
    30961 ctaagtattc tttaggtaaa gcaatcttca ttcctttgat gtcgcctgtc agtccctctg
    31021 caaaatcagg aactgaaacg cctgaagaag taccgtcttt ttcgtcataa ccgctgatag
    31081 ctgtcaatgc taatgcatta tctttcaccg tacgggtcat tggtccgatt tggtcaagag
    31141 atgaagcaaa ggcaatcaaa ccaaaacgtg agacacggcc ataagtaggt ttcatcccta
    31201 cgatgccgtt aaaagctgct ggttggcgga tacttccccc agtatcacta cctaaagaaa
    31261 ctggaatttg tccagctgca actgctgctg cagaaccacc agaagagcca ccaggtactt
    31321 ttgttgggtc ccaagcattt tttgttttct tgaaataaga agtttctgta cttccaccca
    31381 tagcaaattc atccatgttt agcttaccaa cagggatcat atctgcttga tagattttat
    31441 ccatgaccgt tgcatcataa attggttcaa aattgtaaag gatcttagat gcagcggttg
    31501 tcaagatatc ttttgttacg atattatcct tgataccaat tggaatacca gcaaggacgt
    31561 tgctttcatc aatccctttt tcatctaaag cttttgcctg ttccaatgct ttttcttcgt
    31621 tcaatgtaat gaatgcctcg acctcaggct ctgtttcttt gattcgttca aatgttgttt
    31681 ttgtcaaatc ttgtacagtg atttctttcg atacaagcat atgatgcagt tcttctagag
    31741 aacgattgtg taattctgtc attatgcgcc agcctcccca ttatcgatga ttgcgggtac
    31801 tttgataaag ccgtcttctg tttccgtcac atttttcaat aattcatcgc ggttccatcc
    31861 agcttgcgcc cgatcttgtc gcatcacgtt tactgtttca accacattcg acgtaaatgg
    31921 aacaccttcc gtatctactt cttccagcat ttcaaccata tcgatgattt tacctaattg
    31981 gtcggtaaaa cttgcaagtt cttcatctgc gaacgcaagt tttgctaatt tggcaacgtg
    32041 tttcacttgt tcttcactaa tcgccatact accccttctt tctaaacgag ttaaaaactt
    32101 tgctctctat tttaacacaa gagtgccgtg tctgaaattg tttattgaaa tttgtctgaa
    32161 aaaactcatt tttcattctt ctactgagcg acttttagcc aatgcatctg ccatttgatc
    32221 ttctgtccaa acttcgattc ctaattcttg tgctttggtc agcttgctgc cagcatcttc
    32281 tcctgctacc acgatatccg tttttttcga tacgctacca gtcactttcc cgccaagatt
    32341 ttcgatcgtc tcttttgctt cttcacgagt aaatcgtgtt agtttaccag taaggactac
    32401 tgttttttct ttaaaaggag actcgacttc ttgcaattgg gcatttcgaa gccctttgta
    32461 ttcgaaattg acgcctgctt gttttaattc attcatcaat tcatggactt cttcgttact
    32521 gaaataagtg actacactgt cggcaatgat atcaccgatc gtatcaagcg caataatcga
    32581 ctcatagtca cttttactca atgtttctag atcaccgaaa tgttctgcta aaatttttgc
    32641 tgctttcgct ccgacatgtc ggattcctaa cccgaaaatc aaccgctcga ccgaattatc
    32701 tttgctgttg tcgattgctg tcaaaatatt atttgctgat ttttctttta ttttgtccaa
    32761 cgtcaataac tcttcttctg tcaatttgta aagatcagca acatctgcta ctagtttttt
    32821 atcatacatc tgttctagta cgcgcggccc caacccatcg atattcatcg catttcttga
    32881 aacaaagtgg ttcaaacctt ctttcatttg agcagggcat ttaggattga tgcaccgtaa
    32941 ggctacttct tcatccaaat ggacaagttc acttccacaa actggacaat gtgtaggcaa
    33001 ttggtattct tcgctgtctt taggtcgttt atctaacacg acttgcgaaa cttctgggat
    33061 gatgtctccc gctttataaa tcagaactgt atctttcaag cggatatcct tcaacttaat
    33121 ataatcaccg ttatgcaggc tggcacggct tactgtcgtt cctgctactc ggactggtgt
    33181 catgatagct gttggcgtta ctacccctgt tcgaccgact gtccattcga tgttttcgat
    33241 tagcgtttca acttcttctg gcggaaactt gtaagctgct gcccatctcg gtgctttgat
    33301 agtaaaacca agttgatctt gtagcgagaa ttcattcact ttgatgacga ttccatcgat
    33361 ttcatagggc aaatccacac gtttgtcatg atattcttca atgtatgacc acacttcatc
    33421 aatcgaatga catagacgtt tttcccgatt cgtgtggaat ccaatttttt ctagctcttc
    33481 tagcgcatca tactgggttt ttgcttgcat aggaccgaaa tcagctactg tgtataaaaa
    33541 agtgtccaga tttcttttcg ctgtgatttt agaatctaac tgacgcaaag aaccagctgc
    33601 ggcgttccga ggattcgcaa agatatcttt tccctctgct tctctgtctt ggttcaattg
    33661 gacaaatgac cgttttggca tgaaacattc gcctcttact tcgatgttca ttggttcttt
    33721 taatttgatt gggaccgacc ggacagtttt taggttttca gtgatgtttt ctcctacagt
    33781 tccatccccg cgagtcgcac ctctcacgaa atttccatct tcataacgca aagaaacgga
    33841 aagtccatct atttttagtt cacagcaata gtcaactaca cgtcccactg ctttttgtac
    33901 acgttgatca aaagctatca gctcttcttt gctgaaaacg tcgttcaaac tgtaaagcgg
    33961 aatatcatga gtgacttttt caaaaccttc taatactttt ccgcctactc gttgagtggg
    34021 agaatcagac gtgatcaaat caggatattg ttcctcgatt gctactaatt ctgcgtattc
    34081 tttatcataa acatagtctt ccactgttgg tttatcttct acgtagtatt cgcgagacca
    34141 ttggttcagt cttgtacgaa gttcttctgc tcgtttagca gcttcgtcaa tcgtcatttc
    34201 atgttccatc atttaccctc cactactaag ctttttcaat tggcgcaaag gcagccaaaa
    34261 gacgttttac tccttgactt gggaaagcca catctagttc tagatccttc gacgtcccgc
    34321 ttactcggac gactgttcct agaccccatt ttttgtgttt gactttatct ccaggtttcc
    34381 agctgttatt ttcccctcca gtagcacttt tagagggaac aaccgctgtc tctggctgtt
    34441 tatagctata tgctgttttt ttagcgaata caggcgtttt tttaggcgta ggacgcatgc
    34501 cttcgatttc cagtaactcc tgatcgattt cttctacaaa acgagaaggt cggttatatt
    34561 gcgtccgtcc atataatgtt ctggagaaag catttgttaa atacaacgct tcttctgctc
    34621 gcgtgatccc cacatatgcg agacggcgtt cttcctccag ttcgctttct tccatcaatg
    34681 ctcgagataa aggaaaaacc ccctcttcta gaccaatcag gaatacaact ggaaattcta
    34741 atcctttggc tgcatgcaaa gtcatcaatg tcacttggga agcatcttct tctaaattgt
    34801 caatgtcgga aacaagtgcc agatcattta agaagaccgt tagtttttct tctggtgcat
    34861 cagcgtcttc ttcattttgc tgttcgaatt gtttgtcgaa ttcttgtgtg accgttaaaa
    34921 attcctccaa gttttcgatg cgtgcttgtg cttctaatgt attttggatt tttaagtctt
    34981 ctagatatcc gctacgatcc aaaacctctt tcgtcaattc tgtcactgtc aagtatggga
    35041 tcatctgagt gacttcttgg atcatctctc caaatgcacc taattgctgt cctgcttttc
    35101 caccgatatt tgccagatcc acattttgcg ctgcttcaag cagtggccat tcatgaagag
    35161 aagcaaaact gcgtagtttt tcgatagatc ctggtccgat gcctcgtttg ggcgaattaa
    35221 cgatacgctc aaaactgatt gaatcttgag gatttaccag cacattcaaa tacgctaaaa
    35281 tatctttgat ttctttacga tcgtagaact tgtgccctcc aaccatttta taaggaatat
    35341 ttgctttcaa cagagtttct tccattacac gagattgtgc gttcgtccgg tacaagatgg
    35401 caaagtcgcc gtaattccga tgattgcttc gaatctcttc ttgcatccgg tctacgataa
    35461 aacgggtttc gtctcgttca ttatctgcac ggtagtaagt gatcttgttt ccttctttgt
    35521 tttctgtcca caagtttttt ggttttcgat tgctattgtt ttcaatgact tggtttgctg
    35581 cgctaaggat gtttttcgtg gagcgatagt tttgttctag caaaattact gcagcatctg
    35641 gataatcttt ttccaaatcc aagatgttct gcatatctgc accgcgccag ccgtaaatac
    35701 tttgatctgc atctcccacg acgcacaggt tcctaaatct acctgccaat aaattgacta
    35761 gcgtatactg tgcgtgattt gtatcttggt actcatctac atgaatataa tggaatttgt
    35821 tttgataata cgtgagtgaa tcaggatgtt cttcaaataa tcgaatcgta ttcatgatca
    35881 ggtcgtcaaa gtccatacat tgattatatt gaagctcttt ttggtaagca gcataacatt
    35941 ttgccgcaat ctcttcgtaa taactgcctt gcatttttgc aaaatcctga ggggtctgca
    36001 ggctgttttt tgcttgagag attgttccta atatacttcg aggatcatat tttttcggat
    36061 caatgttcag ttcttttagt atccgtttca tcaaggtcag ctgttcagaa gaatcgatga
    36121 ttgtaaagtt ccggttatac ccgataaaat caacatctct tcttaaaata cgcacacaca
    36181 tcgagtgaaa agtcgatacc cagacatctt cgcctccaga tgcgagaatc gcattgacac
    36241 gttctttcat ttcttttgca gccttattgg taaaagtaat cgctaggatg ttccaaggat
    36301 tgacttcttt ttcctcaatc aaataggcaa tgcggtgtgt cagcactctc gtctttccac
    36361 ttcctgcacc tgccattagc agtaatggtc cgtcagtgtg aagaacagct tctttctgac
    36421 gggggttcat cccgtttaat aattcagctt tggatgacat taacaacatc ctttctttta
    36481 tcaatcttgt taattatagc attttcttgg ctttcattaa actagctagt tcaaaatcat
    36541 ttgtttcttt ttatggattt ttccttgtca attcgttgat ttcgcgctaa attaaaagaa
    36601 agcgaggcgt ttgaatggaa aaaaaaggaa aaatcaagaa attagataag actcagatat
    36661 attttattct aaaaggaata tttgttggac tgctgacagg aatcgtcgtt agcctgtttc
    36721 gattatctat cgaaaagcta agcgatttag ttcgaagtat ctatgaaatg agtcgggaac
    36781 agccgattta cttaattggc ctagctattt gctgcatcct tgcagcattt ttcgtgggtt
    36841 atctggtcaa aaatgaaccg gatatcaagg gaagcgggat tccacaagta gagggacagc
    36901 ttcgcgggga attatcaatg aactggttct ctgttttatg gaaaaagttc attggcggtg
    36961 tactttcagt tggtgcgggc ctttttctag gtagagaagg accttctata cagcttggcg
    37021 catccattgg acaaggagct ggacaattgt ttcggagtcc atcttcagaa gaaaagattt
    37081 taatttctag cggtgcaagt agcggacttg cagcagcatt caatgctcct atcgcaggat
    37141 tactctttgt tttagaagaa atccatcact ccttttctcc acttgtttgg ctgacatcat
    37201 ttgcttcagc aatcactgct aacttcgtct ctctttactt tttcggcttg agacctgttt
    37261 tatatctagg tgaccttcct tctttgccgt tgaaatatta tggttcctta gtgatcttag
    37321 gagtacttct aggcatctta ggttttattt atcagaatgt cttgctggct ttgccaaaat
    37381 tttacaaccg attgccttta ccagcttatt ttcacgggtt cgttcctttc ttgctgattt
    37441 tgccaattgg ttatttatgg cctagtactc taggcggagg caatcaaatc gttttagctt
    37501 ttggacatgc ttccttgcca atcattatga ttatcggttt gtttgttctt cgattcgttt
    37561 tttccatgat ttcttacggt gggggtcttc caggggggat tttccttccg atattgacat
    37621 tgggggcttt aatcggtagc ttttatggaa acattatggt tgatttcggg atggatccaa
    37681 tctacatcaa ggattttgtc gttattgcca tggctggtta tttcacagcg attggaaaag
    37741 caccgctgac agcaattatt ttagtgacag aaatggttgg ctctcttaat catctgatgc
    37801 ctttaggatt ggctgctctt gttgcttata tcgtcaatga ccttctcggc ggtaatccaa
    37861 tttacgaatc attattggaa cgtctacttg gaaatactca gaccgcctca ctaaaaggtg
    37921 caaggacaac ttttgatttt ccagtaacgg cagagagttc tttagacgga ttaatggtac
    37981 gagacttcaa ttggcctaaa aatatgctgc taatctctat acgcagagga aatcaggaaa
    38041 tcttgactca cggtgatacg atcatgaatg taggagatat tttggttatc ttgactgatg
    38101 aagcaaactt gccaagaatc aagcaggaaa tcgaggctaa atcttctatc acaaatttga
    38161 agcaatcatt ttaacgaaca tggaaaaact attttacaac aactattcat ctacactact
    38221 tgattctata tgattctata aaaaaagaag aggctgggaa aacccagcct cttctttttt
    38281 atttctatgc ttctgctact gcaggagctt ttctcaataa accataaatc acaccagata
    38341 cgactgctcc gatagcaata aacaccaaat acatgaccgg gtgactcagt aagaatacaa
    38401 cgaaaatccc gccgtgagga gccagtaatt tgattccgaa agctccgact aatccgccag
    38461 ttaaagcaga accaataata aaacttggaa tggcacgaat cgggtcagct gctgcaaatg
    38521 gaatcgatcc ttccgtgaca aacgatagtc ccatcacgat gttcgtcaaa ccagcatctt
    38581 gatctgtttt gctgaattta ttcttaaaca aacgtgtagc aacgaaaatt gccaatggag
    38641 gtaccattcc tccagccata acagaagcca tcacgacact tccgccagtt gcaacagtcg
    38701 tagctaaagt tgctgtacca aacacataag cggctttgtt gattggaccg cctaaatctg
    38761 ccgccatcat tccagctaat aatgcaccaa gtaaagcagc attcgaacca ctcatacttg
    38821 tcaagaaacc gttcaatgca tcattgattg ctttcatagg gacattgacc agcaacatca
    38881 agaaaccagt aatcagtaag ccaaacactg gataaaagag gatcgtttta attccatcca
    38941 atgattttgg taagttcttg aataatttac gtaaaaatac gatgacataa ccagctaaga
    39001 atccaccaat cagtgcacct aagaatcctg caccgcctgt attagctaat gcaccagcag
    39061 caaatccaac gatcaaacca gggcggtcac cgatacttga agcaataaat cctgctaata
    39121 ccggcagcat gaaaccaaat gcagcttgtc cgatttggtt gaaccagcta gcagcttgat
    39181 tgtaattccc tagatttgat aattgatcat gtggcacgcc aataaattgg tcgatcatga
    39241 aagaaagcgc aatagcgatt cctccaccaa taacgaaagg taacatgtgt gatacgccgt
    39301 tcatcaaatc tttgtaaatt ctttgtccga tagtaccatc cgcatttcct tcttctgaac
    39361 tgctttcttt tccatcacca tggaatactg gtgcagtacc actaagggct agattgatca
    39421 actcttctgg tttacggata ccatcactga caggacgatt gatcagttct tttccgtcaa
    39481 agcgattcat ctctactttt ttgtctgctg caacgattac accttctgca cgggcgatgt
    39541 cttcagcagt caatcggttt ttgattcctt ctgaaccgtt cgtttcgact ttgatctcca
    39601 cacccatttc tttcgctttt ttcttcaaag catcttctgc catgtaagtg tgagcgatcc
    39661 ctgttggaca agctgtaacg gcaacaacga attttctttc tggatgagca gaagcttctg
    39721 cagcttgtgt ttcttgttct gctttttctt ctgcttcttt ttgctcttca gctgtttgga
    39781 acaagtcttg gacttcttct ggtgtatttg cttcttttaa ttttcctacg aaatctgcat
    39841 caatcagcaa tcgagatagt gctgctaatg cttgcaaatg tgtgtcgttt gcgccttctg
    39901 gagctgcgat catgaaaaac agataagttg gctgtccgtc taaggcttca taatctactc
    39961 ctttttggct ttttgcgaat aaaactgttg cttctttaac tgcactgttt ttcgcgtgag
    40021 gcatggcgat tccatcacca agacctgtag aagtttgtgc ttcgcgggct agaattcctt
    40081 ctttatatgt ttcgatatcg gaaatccggc cggcatcaaa cattttttgg accatttcat
    40141 cgattgcgcc ttttttatca gtcgcttgca aatccatgat catcgcttct ttgaccatta
    40201 aatccttgat attcatgatt atcctccttg aaactaatca atttttgtga tttcaacttc
    40261 tggcagcaac tcatcaatca attcgcgagc tgccaagtca tcagaaaaag ctgttgcgct
    40321 tccgcaagca actccccatt taaaggcttc gattggatct ttcgtacggc tgtaagcccc
    40381 aataaagcca gcaatcattg aatcccctgc acctactgaa tttttcaatg ggcgaactaa
    40441 tacattcgtt ttataagttc cttgttccgt gaagagcaaa gcaccgtctc cagccattga
    40501 gatcagcaca ttctgtgccc cttcagctag taattttttc ccatacggaa tgatttcttc
    40561 tacagattgg aaggttgtgt tgtaaagctc agctaattcg tgattgtttg gtttgatcaa
    40621 caacggtttc ttaacaagtg cattttttag gtcatcacct gttgtatcaa tcacgaaatc
    40681 tgcacctttt tctcgaatga tatcaatcag ttcttgataa aatccttttc gcaggctagc
    40741 tggcgcgctt cctgacaata cgacgatatc tccttgtcct actgttccta atgcttgctt
    40801 caactcatcg atttcttcat ctgaaataga tgggcctaag ccattgatct cagtttcagt
    40861 gtctgatttt agtttgatat tgattcttgt atcttctttt acggttgtga acaaagactg
    40921 gatttgttct ttttcaagcc acttcgaaat aaattcgcca gtgaaccctc ctaaaaaccc
    40981 tagtgctgta gattgagaat cgatccgttt caaaatacgg gaaacattga tcccttttcc
    41041 tccaggtaac ttgaagtctt ccttcattcg gttcaaatcg cctagtttta acccatctac
    41101 gcgtacgata taatcaattg atggattaag tgttactgtg taaatcattg tattgcctcc
    41161 ttaattgttg tcttttcacg gaattcatta acgaattcta atggacaacg ctcggttatg
    41221 atcgtggctg cttccaaagg tgcaacagag gtgaaggaca actggttgaa tttggtatga
    41281 tcaatcaata caaatgtctc ttcggaattt ttcatggcta aacgtttaac agctgcctct
    41341 tctggatccg gagtcgtgaa tccttgttca agatgggcac cgttcattcc cataaatgct
    41401 ttattaaaat gaaaattttt tagttgttct atcgcattag atcctaaaac agcattagtt
    41461 gccagcttta tcgttccacc taaaataatg gtagcaagct ccaaatcaac taaatttgcc
    41521 gcatgttgaa cggaatttgt cactactgtg atctgttttc cttgcagaaa aggaatcatt
    41581 tctaacgtag tagagccagc gtctaaataa atgacatctt cgtcattaat ggtttcagct
    41641 gccgatttgg cgatttgctg tttttcgtga acgtttttga gtgatttttc actcatattc
    41701 tgctcaaaat tcaataattg ctctttctta gctccgccat gaactctttc tagaaaccca
    41761 ctattctcca gctcttgtaa atcacgtctt atcgttgatt cagaagcatt caaaaggttt
    41821 acaagatctt gtgatttcac gattttctgc tgttcaagca agtctaggat tttttgtctg
    41881 cgttcctctg taagcatttt cacgatcacc tcaatacaaa taatagcata ttttttttct
    41941 actagcaata taaatcagtc aaaaacgttc ataaaaatta taaatcagtc aaaaaaaaaa
    42001 taaaaaacca gtcagaaaga ctggtttttt atttttttgt ctatcatcaa gccgttttat
    42061 cgttttttat tttcgcttct tgtttttttc atataaacag aagcaggttt tctttcctta
    42121 tgaagaagat aataataaag tcccatcccg ccaaacatga ccaaaaaacc gatgactgat
    42181 cgaataggaa aattaggaac aaaaaagaga cagacggata ataatgatgc cggaaccatt
    42241 attcgataat agagttgttt atccattttc ttcacctcat attttttgta aagtaatcaa
    42301 ttgttctaat tctcggtaat ctaaaaattg cacacgcaga tgctgctcac ctttcttgac
    42361 atcgaattgg atacttcgcc gctttttctt tattcgtttg aaggtaacat cagagagtct
    42421 atgtttgatt tcctttaacg gtaattctga cagggattct gaaggaacat agattcttaa
    42481 ggataaaaga tactcttgat atttttcatc tgttacctct acttctagat attccctaat
    42541 ataataacaa taagcttcga ttccattatc caaagcaaca gtcggatcga taactttata
    42601 gcacatcatg cgtgatcgat tctcgtcaac ccaaggaaaa tacgcctgag catttagtgt
    42661 tccgcgaaca cgcctttctt tataatctcc ttgttggata tcataacttt ttgttacaaa
    42721 aaatgaagca ttctttttag gaacgggttg catttgtgca ttctgatact ggacagattg
    42781 gatcgtatgg tcagtgtaaa tgtattcaac gaatggcggt ttcaataaac cgacaccatt
    42841 gtcccaagca agatgatcaa acccaagctg gtccctaaaa taggattgaa acatttgatt
    42901 gtttggcttt tgccagttaa aggggtctcg ataaagctgg atacatatga cttcgttttc
    42961 taattccttc actttttcta cttgttggat gtcgagaaaa atcggtttag ggatgatttg
    43021 atgataatag cgactataat acatttccca acacgaagtg aaacataaac cttggtaaaa
    43081 aagatcgtat cctggaaatt ggttgcaatc gactactact tccccgtttt gttgtttcat
    43141 tttaggtaaa tgttcgattt catccttttt ctcaaacaat agccgttgag agatctgttt
    43201 tgtattgtga tagataaact cgctataagc tcgcatatag gcgaacaccc catgagcaat
    43261 catttttttc tgaatgaagt ctcgaatcaa aaattgcttt tctttgaaaa cagagataga
    43321 caatacattt tttatcaata attgattatc ttccaagcga aaactacatt cgttctcgtc
    43381 atttcctaca actaaccaac tttcgtctct taccgatgct agcattattt tttcttgaag
    43441 ctttcgaaga tcatacggat ccgcttgttg tgtgaaagag cggataaatg ttggctggat
    43501 caacagacgt gtcaaaatag cccaatcgct tatttcctgt tttttttctt cttcatttaa
    43561 gtctgtgagc tggatagtca tcaataattt atcttcgcca atcattttct cacctctttc
    43621 aaatcttttt ataatttaat tataaccaat gataaagaaa aactaaaact ttatgtctta
    43681 gaaaccaatt tggacgatta catgatagac taagtaaaaa gaaacaaaga ggtgtttaca
    43741 atggggttga aatttgaacg tacagataat ttagataaac ttttcgaatc atttgctgtc
    43801 gatccggaaa aaacagttgc accaaaagaa gagatcgatc gatttctaaa accacagcca
    43861 caaaatgaaa agaaaaaaga caagaaacaa aagtaagtta agc
//
LOCUS       DACEUZ010000008        38176 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.008, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000008 DACEUZ010000000
VERSION     DACEUZ010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38176
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.008"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            308..1192
                     /locus_tag="IXG44_000378"
     CDS             308..1192
                     /locus_tag="IXG44_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1657120.1"
                     /translation="MAFETIGEKLRQARLNKKISLDELQQITKIQKRYLEAIDNDDFD
                     QLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQPETVSTSRKAMHQ
                     EESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDTASSLKVEGSVEES
                     SSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITDATKPVTLEFTAVN
                     RVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNATIKANGESVPVNP
                     GENNQSPKNVNLTLQYAE"
     gene            1401..1979
                     /gene="pgsA"
                     /locus_tag="IXG44_000379"
     CDS             1401..1979
                     /gene="pgsA"
                     /locus_tag="IXG44_000379"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HAQ1657121.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            2219..3460
                     /locus_tag="IXG44_000380"
     CDS             2219..3460
                     /locus_tag="IXG44_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HAQ1657122.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            3569..4618
                     /gene="recA"
                     /locus_tag="IXG44_000381"
     CDS             3569..4618
                     /gene="recA"
                     /locus_tag="IXG44_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HAQ1657123.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            5029..6585
                     /gene="rny"
                     /locus_tag="IXG44_000382"
     CDS             5029..6585
                     /gene="rny"
                     /locus_tag="IXG44_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HAQ1657124.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(6664..7470)
                     /gene="proC"
                     /locus_tag="IXG44_000383"
     CDS             complement(6664..7470)
                     /gene="proC"
                     /locus_tag="IXG44_000383"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HAQ1657125.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLTDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            7611..10184
                     /gene="mprF"
                     /locus_tag="IXG44_000384"
     CDS             7611..10184
                     /gene="mprF"
                     /locus_tag="IXG44_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAQ1657126.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            10338..11189
                     /locus_tag="IXG44_000385"
     CDS             10338..11189
                     /locus_tag="IXG44_000385"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HAQ1657127.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            11434..12372
                     /locus_tag="IXG44_000386"
     CDS             11434..12372
                     /locus_tag="IXG44_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HAQ1657128.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            12395..13075
                     /locus_tag="IXG44_000387"
     CDS             12395..13075
                     /locus_tag="IXG44_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAQ1657129.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            13048..13872
                     /locus_tag="IXG44_000388"
     CDS             13048..13872
                     /locus_tag="IXG44_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAQ1657130.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            14039..14857
                     /gene="purR"
                     /locus_tag="IXG44_000389"
     CDS             14039..14857
                     /gene="purR"
                     /locus_tag="IXG44_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HAQ1657131.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            15360..16733
                     /gene="glmU"
                     /locus_tag="IXG44_000390"
     CDS             15360..16733
                     /gene="glmU"
                     /locus_tag="IXG44_000390"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HAQ1657132.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            16827..17798
                     /locus_tag="IXG44_000391"
     CDS             16827..17798
                     /locus_tag="IXG44_000391"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAQ1657133.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     regulatory      17804..17906
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            17972..18541
                     /gene="thiT"
                     /locus_tag="IXG44_000392"
     CDS             17972..18541
                     /gene="thiT"
                     /locus_tag="IXG44_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HAQ1657134.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNSWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     gene            complement(18622..19395)
                     /locus_tag="IXG44_000393"
     CDS             complement(18622..19395)
                     /locus_tag="IXG44_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1657135.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            19661..21361
                     /locus_tag="IXG44_000394"
     CDS             19661..21361
                     /locus_tag="IXG44_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HAQ1657136.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            21331..21549
                     /locus_tag="IXG44_000395"
     CDS             21331..21549
                     /locus_tag="IXG44_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1657137.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            21569..22459
                     /locus_tag="IXG44_000396"
     CDS             21569..22459
                     /locus_tag="IXG44_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HAQ1657138.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFALDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            22477..22761
                     /locus_tag="IXG44_000397"
     CDS             22477..22761
                     /locus_tag="IXG44_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1657139.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            23248..23862
                     /gene="gmk"
                     /locus_tag="IXG44_000398"
     CDS             23248..23862
                     /gene="gmk"
                     /locus_tag="IXG44_000398"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HAQ1657140.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEERMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            23867..24172
                     /locus_tag="IXG44_000399"
     CDS             23867..24172
                     /locus_tag="IXG44_000399"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAQ1657141.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            24425..26833
                     /gene="priA"
                     /locus_tag="IXG44_000400"
     CDS             24425..26833
                     /gene="priA"
                     /locus_tag="IXG44_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HAQ1657142.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            26848..27339
                     /gene="def"
                     /locus_tag="IXG44_000401"
     CDS             26848..27339
                     /gene="def"
                     /locus_tag="IXG44_000401"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAQ1657143.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            27332..28270
                     /locus_tag="IXG44_000402"
     CDS             27332..28270
                     /locus_tag="IXG44_000402"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HAQ1657144.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKEAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            28270..29628
                     /gene="rsmB"
                     /locus_tag="IXG44_000403"
     CDS             28270..29628
                     /gene="rsmB"
                     /locus_tag="IXG44_000403"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HAQ1657145.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKTLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            29641..30381
                     /locus_tag="IXG44_000404"
     CDS             29641..30381
                     /locus_tag="IXG44_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HAQ1657146.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            30378..32447
                     /gene="pknB"
                     /locus_tag="IXG44_000405"
     CDS             30378..32447
                     /gene="pknB"
                     /locus_tag="IXG44_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HAQ1657147.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            32612..33511
                     /gene="rsgA"
                     /locus_tag="IXG44_000406"
     CDS             32612..33511
                     /gene="rsgA"
                     /locus_tag="IXG44_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HAQ1657148.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            33524..34174
                     /locus_tag="IXG44_000407"
     CDS             33524..34174
                     /locus_tag="IXG44_000407"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAQ1657149.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGQFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            34175..34813
                     /locus_tag="IXG44_000408"
     CDS             34175..34813
                     /locus_tag="IXG44_000408"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HAQ1657150.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            34968..35822
                     /gene="mreC"
                     /locus_tag="IXG44_000409"
     CDS             34968..35822
                     /gene="mreC"
                     /locus_tag="IXG44_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HAQ1657151.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            35826..36335
                     /gene="mreD"
                     /locus_tag="IXG44_000410"
     CDS             35826..36335
                     /gene="mreD"
                     /locus_tag="IXG44_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HAQ1657152.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            36590..38128
                     /locus_tag="IXG44_000411"
     CDS             36590..38128
                     /locus_tag="IXG44_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1657153.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTVPESSATEE
                     SKTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTPEQPKPVTPAPAP
                     SGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTGRDIGGWTVPQES
                     AGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESVKVGSVQWFAPDF
                     AVSM"
BASE COUNT        12553 a         6828 c         8305 g        10490 t
ORIGIN      
        1 acggaaaaat atctccttat attttgccgg tttttgagct aataccgagg aacagctgat
       61 tgaacaccgt ttaagaaaga ggtcgtgagc ctgccgttta actccgagtc acaaggaaca
      121 agttcaaaaa acagcttctt atatttattg gatattgtga cttgtgaccg aggagttggc
      181 aggagaacaa tgtttattcg gaaaaaagga gctgtgacat gacttttgtc acagcaccca
      241 aacataacgt ctttcgttgc aacagcagat gcttcggaga atcaaacaga ctggagaaga
      301 tgaacgagtg gcttttgaaa ccatcggaga aaaattacgg caagcacgcc tgaataaaaa
      361 aatatcttta gatgaattac agcagattac aaaaattcaa aaacgttatt tggaagcaat
      421 cgacaatgat gattttgacc aattgcctgg taaattttac gtgcgggcat ttattcggca
      481 atacgctgaa gctgtaggag aagatggaga ccatttagtc gatgtttttg atgggaaaag
      541 caaattgaca ggatcggtcc ctgaacgtcc gcagcctgaa acagtaagta cttctagaaa
      601 agcaatgcat caggaagaat cgcatccaag tcgcttttgg acaagtcttc ctgtcatttt
      661 attaggattg gttgctttgg cgattgtcgt tgtcgtgggc tatatgactt ggcaagatcg
      721 tcaagctgat ccaataatcg gagacacagc ttcttcgctt aaagtagaag gatcagtcga
      781 ggagtcctct tcctcagagc aaagcacgca gaccaaagag tcttctacag tagaatcaac
      841 aacaaaatca agtgaagaaa agaaaatggc aatagctaca ggagaagatt ctggcagtgc
      901 catcgcagta aatatcacag acgctaccaa gcctgtaacc cttgaattta ctgctgtcaa
      961 tcgtgtatgg ttaggtgtgc tagtagacaa tgcctatgtt tatcaaggga cactggcagc
     1021 aaatgaaaca cagagtaccg tgttaccgga aacagcaaca aatgcgacca tcacgattgg
     1081 tgcagcaagc aatgcaacga tcaaagcgaa tggagaatcc gtaccagtta atccagggga
     1141 aaacaatcag agtccaaaaa atgtgaacct tactcttcaa tacgcagaat gatcgtataa
     1201 attagtttac gcatctgttt acttgtaggt ataaaaaaga aggatcgtga caaatctgtg
     1261 tcgcggtcct tcttcgttaa ttcaaaaaga tcaatttcaa taaaaatact cctttgaata
     1321 aattcatttg gaaaagtaaa taatatcggt ggaaacttcg ctgatagtca cgtagaatag
     1381 ggaagaaagg agtcgatagt gtgaatttac caaataaatt aactgtactt agaatttgta
     1441 tgatcccgat tttcattttg gttgtaggtt tacctttaga ttggggacaa tttaacgtag
     1501 gagacaccac actggctgtc acacagctag taggtgcagt tatttttgct ttagctagta
     1561 tcacagactg gttagatgga aaaattgccc gtgcgcgagg ccttgtcact aattttggca
     1621 agtttgcgga cccgttggca gataaaatgc tagttatgac tgcttttatc atgttggttg
     1681 accaaggaaa ggcccctggt tgggtagtag cgatcattgt ctgtcgtgaa ttagccgtca
     1741 caggcttgcg tttactactt gtcgaacatg gagaagtcat ggctgcagct tggccgggaa
     1801 aagtcaagac ggctacacaa atggttgcta ttatcttgct gtttatcaat aatatcccat
     1861 tcagcctgat cggtattcca gtagatcaaa tcatgcttta tgcttgtctg atctttacta
     1921 tctattcagg tgtggattac ttcatcaaaa acagcggtgt gttcaaagga tcaatgtaag
     1981 tatcgtttag tggagaggtt gtgagcctgc cgtctaactc tgagtcacaa gggataattt
     2041 caaaaaacag cttttcatgt tttatgtata ttgtgactta tgaccgaggt tttggcagga
     2101 gaacactgtt tatttggaac aaggagctgt gacaagattt ttgtcacagc tccttgccct
     2161 ctttttaaat aaaaaaatat aaaaatacag cgtatactat ataagggagc gggaagtaat
     2221 gaaagcagaa ataatagctg tcggaacaga acttttacta ggacaagtcg tgaatacgaa
     2281 tgccactttc ttgtcagaac agctagctga tctaggtatc gaagtttatt atcaaacagt
     2341 tgtaggagat aatcagcagc gtttagaaga gttgattgct ctagctgaaa caagaagtga
     2401 actcatactc ttatgtggcg gattaggacc gacagaagat gacctgacaa aagaagcaac
     2461 tgcggctcac ctcggtaagt ctctcataca aaatacggaa ggatacaaaa aattattggc
     2521 atattttgaa acaacccatc gaaaaatgac caaaaacaat ctgcaacagt cccagattat
     2581 cgaaggcggg gttcctctac caaatcgtac agggcttgct ttagggactt tttatcaaac
     2641 agatacacat gcgtatatcc ttcttcccgg tccgcctaat gagttgaaac caatgtttgt
     2701 ggaacaagta cgtcctttac tagaggaacg ttttccttct gaagaaaaac taatttctaa
     2761 agttttgcgc ttttatggga ttggtgaatc gcgactggtc actgagctaa aagatctgat
     2821 tgagacacag atcaatccaa cgatcgcacc atatgccaaa ccaaatgaag tgacattacg
     2881 actgacagtg aaaacaaatg atgttcaggc agggaatcaa gctcttttag cattagaaga
     2941 aaaaatccaa gaacgagtag gggaatattt ttatggatac ggcgatgata attcgctagc
     3001 aaaagtcgta gtggaactac tgaaagaaaa caagcagact gtaacagcag cggaaagttt
     3061 gacagccggc gccttccaag ccgcattagg cgacattgct ggtgtatctg aagtatttcc
     3121 aggcggattc gttacctact cgctccaaac aaaagctggt tttctggaga ttgatccgga
     3181 gctattagca gaatatggaa cagtcagcaa agaatgtgtg gagcagatgg ccattcaagc
     3241 acgtgagata gcaagaacag attacagtgt agctttcacc ggagtggctg gaccggacac
     3301 gttagaaggt caatctgcag gaacagtatg gatcgctatt tcatctaaag acggcgtggt
     3361 ttcggaaaaa tatcactttg ccagagatcg tgctgctatc agatataatg cggttatgca
     3421 aggatttgat cttttgagaa aacagctttt aagaaaatga taaatgcgaa tatttgttcg
     3481 gttttttctt gctttttttc actaaaaaag atatgataag gataatgaaa tgttgctctg
     3541 atttgtaaat ataaggagga ttatcgtatt ggcagatgat cgtaaagcag ccttagatgc
     3601 tgcactgaaa aaaattgaaa agagctatgg taaaggctct attatgaaat tgggcgaaaa
     3661 aatcgaccaa caaatctcta caattccaag tggctctctt gcgttagatg tcgctttagg
     3721 tgtcggcggt tacccccgtg gacgtatcat cgaagtatat ggacctgaaa gttcaggtaa
     3781 aacaacagtt gcactacacg ctattgcaga agtacaaaaa aatggcggaa cggccgcttt
     3841 cattgatgct gagcatgcgt tagatccgca atatgcacaa aaattaggtg tgaatatcga
     3901 tgaactactt ctttcacagc ctgacacagg agaacaaggt ctagagatcg ctgatgcttt
     3961 agtatcaagt ggggctgtag atatcgtagt agtcgattca gttgctgctt tagttccacg
     4021 agcagaaatc gacggcgaaa tgggtgactc acatgtcggg ttacaagcac gtttgatgtc
     4081 tcaagcattg cgtaaactct ctggttcgat caacaaaaca aaaacaatcg ctattttcat
     4141 caaccaaatc cgtgaaaaag taggcgtgat gtttgggaat ccagaaatca ctcctggagg
     4201 acgcgcattg aaattctacg caacgatccg tttggaagta cgtcgtgcag aacaattgaa
     4261 acaaggtaca gatattgttg gtaaccgtac aaagatcaaa gtcgtaaaaa acaaagtagc
     4321 acctcctttc aaaatcgctg aagtagatgt catgtatggt ttagggatct cacaagaagg
     4381 agaacttcta gacatggcag tagaaaaaga cattgttgat aaaagcggtg cttggtattc
     4441 ttataaagaa gatcggattg gtcaaggacg ggaaaacgct aaaatctata tggctaatca
     4501 tcctgaaatg atggcagaag tttctgctct agttagagca gcttatggta ttggtgaaga
     4561 agtcgctgtc ccagaagatg aaaaaggaca agaagaattg cctttagtag aagaataaaa
     4621 attatgataa acaaaaacgg ctggtctgaa gagtaggtac tctccttgac ctgccgtttt
     4681 tttattgaat aaagaaatcg tgagttattt aattatacga gtgggtacat aaaataaaaa
     4741 tatctgttca tctacaaaga ataatttaat agaaaagttt ctcaatttaa aggtttacct
     4801 atatgaaact agtgtcattt attttaaaaa ccatcgttta atgaataagt agaaagccgt
     4861 ttctttgttt atttaaagtt gacactacgg ggtacaaaca ttagaataaa gttgtgcatg
     4921 ttttttgtac acatatatgc gaagatgcat attgacgagt ttagaataat ttatcataga
     4981 agctgtatag atgataaaaa tgaaaaagat agtatggagg tggattttat ggtattcaac
     5041 attctcctcg ctatcatcgg tttaattgtc ggtcttggtt tagggtttat ggtcgcaaag
     5101 tcacgtcacg agaaagaaat agctggtgca caatcatcag ctgctggaat catcgatagt
     5161 gcaaaaaaag aagcagaaac tctaaaaaaa gaagcattgt tagaagctaa ggaagaaaat
     5221 cagaaatacc gttctgaagt tgaaagtgag ctgagagaaa gtaagttaga attaaaatct
     5281 caagagaatc gtttgattca acgcgaacaa acgctgaatc gtaaagatga ctcgttagaa
     5341 aaacgcgaga actcacttga aagtaaagaa gaagcactta gttcaaaaca gcaattaatt
     5401 gaagagcgag aaaaagacgt tgaaaaaatg attgaagatc aacaaaaaga actagaaaaa
     5461 attgctggtt tatcaaaaga ggacgcacga gatgtcatca tgaaatcgac tgaagaagag
     5521 ctgaatcatg agttaactct aatggtaaaa gaatctgaac agcgtgccaa agaagaagca
     5581 gatcgcaaag cgaaaaactt attatcatta gcgattcaac gctgcgcagc agatcaagtt
     5641 tcagaaacga ctgtttcagt cgttaccctt ccgaatgatg aaatgaaagg tcgtatcatt
     5701 ggacgagaag gtcgtaatat tcgaacgctt gagacgctga ctggtatcga tttaatcatc
     5761 gatgatacac cggaagcagt tgttctttcc ggattcgatc caatccgaag agaaatcgca
     5821 cggatgacct tggaaaaact gatacaagat ggacgaatcc atccagcgcg tatcgaagaa
     5881 atggtcgaga aatctcgtaa agaaatggat gaacgtattc gcgaatacgg ggaagaggca
     5941 gcgtttgaag tcggtgctca tacacttcat ccagatctaa tcaagatcat cggacgtctg
     6001 cgattcagaa ctagttatgg acaaaacgtc ttgaagcatt cggtcgaggt tgcaaaatta
     6061 gctggtatct tagcagcaga gcttggcgaa gatatccaat tagcaaaacg tgccgggttg
     6121 ctccatgata ttgggaaagc acttgaccat gagattgaag gatctcacgt tgagatcggt
     6181 gcggaattag ctgccaagta caaagaaaac ccagtagtaa tcaacgcgat cgcttctcat
     6241 catggagatg tggaagctac ttctgttatc tctgtccttg tagctgcagc agatgcgctc
     6301 tcagcagcaa gacctggtgc gcgaagtgaa tcattagaaa actacatccg tcgtttaaga
     6361 agtctggaaa atatttcgaa cagcttcgcc ggtgtagaat caagttacgc agttcaagct
     6421 ggacgcgaag ttcgggtaat ggtcaagcca gaagaaatct cggatttaga cgctgttcgt
     6481 ttagtacgtg atattcgtaa acgaatcgaa gaagaactgg attatcctgg acacatcaaa
     6541 gtaatcgtta ttcgggaaac tcgtgcaact gattatgcta aataataaaa aatacaaaag
     6601 aagagccgcg gcaaaagcca cagctcttct ttttttgtaa ggtacgaaaa aacattgcta
     6661 tcattatttt ttattcaaca tctcaagatc tttctcgatc gtcgtattga ttcctttgat
     6721 gattgctggg atgaatcctg cttcttctaa ggcaactaca cctgcaacag ttgtaccgcc
     6781 aggagaagaa acttggtcta caagttccca tggattttta tcacttgaaa ggaatttttg
     6841 acaagaaccc ataattgctt ttgcagcaat ctcagtggct gtctgtttgg acaaaccatt
     6901 cagtacacca gtccgtccca tcgcatcgat cagcatgtaa acgaatgcgg gtgagctgcc
     6961 agcaaggcca atgaatgtac tgaaatctga ttctgctaaa ggatagacgc tgccgattgc
     7021 ttcaaaaatc atcgtagctt gctgaagctg ttcctccgtg gcagcttcat ttttgcaaat
     7081 cgcagaaacg cctgctccta cgctgacatt gacattcggc atcacacgta cgatggaggc
     7141 ctgaggatct tcaagcgctg tttctaaatc tgttaatgat tttcctgaag caatagaaat
     7201 gaataagact ttctggctat attctcttag ttcttccaat actttttcaa tgatttttgg
     7261 tttcacggct aaaagaacga tatctgattg tgatgcaact tcctgattac ttttgcatgc
     7321 atggacgcca gtttcagcag caaatttttg tgcattttca aaatgtccac tggaaacgaa
     7381 cacctcttct ttcgagactg ttcctttttt taataaacca tcaataatag cttgtgccat
     7441 attccctgtg ccgataaatc ctactttcat gttatcctct cctttagcat taggatatct
     7501 ttctttttct atcatcgcaa gcaaagaaca ggtctaaagt ggaaaaatta aaataaataa
     7561 gataacattg gggtatgaca caaaagtata caagttagga atgggaaaat gtgaaaaata
     7621 aacgatactt gatgctgcaa tggataaagg aacatagtct gattttaaaa ctgatttttt
     7681 tcggctccgt gttagtcttt gtggcaaatc aagtcgcaaa tattgctaat ggcatgagtt
     7741 ggcaagatat ttggcaaagg atgggacaac aaagccgcat gacgatcctg ctcatgattc
     7801 tggctggact gctgggtgtc actccgatgt tgttgtatga ttgggtgacg atcagagtct
     7861 tagaaaaaca aggaaaacca aaaatggaaa gaaaagagtt tcttcttgct gcgtgggtaa
     7921 caaacacgat caacaatctc gccggttttg gtggtgtggt aggagcaagt ctgcgggcta
     7981 gtttttatgg aaagcagaca aatagaaaaa tggttttagc cactgtatca aaagtcgcac
     8041 tatttatgct gactggtttg tctgtatggt cgtttcttac atttattgat gtctttttta
     8101 ttcagtcaga aagtattttt cgaagctatt ggatctggct tttcggcggt agttttttag
     8161 caccagtcat tttctttttt gcttatctaa agagaaagac attgttcttc gagttttatc
     8221 caaaaggctt gcttggtctg ctactggcat cctttggaca atggacggga gcccttgcgg
     8281 tatttttgat catcggcaag ttgatgagaa tagatatttc gatgctttcg atctatccta
     8341 tgttcatgat tgctacattg ataggaatgc tgacgatggt cccaggtgga atggggactt
     8401 ttgacgtttt gatgatctta ggaatgagtc aattgtctgt tcggcaagat gtagcagtag
     8461 tttggctatt atattatcgg ttgttttatt atgttctacc ttttatgacg ggacttctat
     8521 tgttcatcac acatacagga gtcaaaatca atcgattttt agataacatt ccgcgactgt
     8581 ttttccaaaa ggttgcgcat atcgtgctcg taagtgctgt ctattttgcg gggatcatga
     8641 tggtacttct atcaacaatc actaatctgt ctgcaatcag tcggctattt caagtattat
     8701 tgccgttctc attcaatttt ttagatcaga cagtgaatct attggttggc tttttattac
     8761 tcggccttgc tagagcactt tcagtaaaag tcaaaaaggc atttctgcct acgattgctt
     8821 tactatcttt tggtatttta aatacaatca cacgtacgtt atcttggcag ttgatattgg
     8881 tatatgtctt gattctgtta gctgtgtggt tatccagaaa agaattttat cgaacaaaat
     8941 tcgtttattc ttggggagct ataatctttg atgctagctt gttcggcttt ttgtttatcg
     9001 tttacgcaat tgcaggctat cacagcggac gctttttagt cggtcgaata acaggaaatc
     9061 attttatttt gtttccttca gatgatgtgt ggttctcagg gcttattggt ttgggcatct
     9121 cattgctagc tttagtggcg ctttatcaat atttagctga agatactacg aagcttggca
     9181 gtgagtacga aaaagaccgt tttgaccgtt tattggaaac atacggaggc acagctacaa
     9241 gccatcagct tcgcttgtca ggttacagtt atttttatta ccaagaagac gaccaagatc
     9301 aagtcgtttt tggttatcag atcaaaggaa acaaatgctt tgttttagga gatccagttg
     9361 gcaatcctaa aatgatgcgg gcagcaacga atcagtttat gaaacaagca gattgtttag
     9421 gctatcagct tgcgttttat aagatatcag aaagctacgt agtgatgctt catgaattgg
     9481 gttttcactt cacgaaagta ggtgaagcag ggattgttga tctagcaaca gaagacttgt
     9541 ctcggctgag tcagcaactt gaatatcaac gtttgatgaa agaaggatac cattttactt
     9601 ggtacgatca actgccggaa gaattacatc aagctgtaaa tgaagtttca caaaaatggc
     9661 ttcaaggtgc acgagaaaaa tacttctctg tgggacgatt tgataaatca tatgtattat
     9721 ctagcggtgt cggtattgtc cgtaaagatc aaaaagtcgt tggcttcatc accgctcagc
     9781 cgatgacgaa gaagcaggcg ggatatgatt tgttgcgggt cttacctaaa gaatctgagg
     9841 aattagctga ctatctgtta gccaatctgt ttattgtata cgagaaaatg ggctactcgg
     9901 aagccaatct aggcttagct ccattagcta atgttggaga aacagatttt tctttctttc
     9961 aagaaaaagt catgcatatc gtatataatt atggtaattt tttctattcc ttccagtctg
    10021 cttatgaaaa gaaattaaaa tatgtcgatc attgggaagg acgttatttt gcttatagga
    10081 aaggaagtaa tttcggattt gctaccatgc agctactcag tctgatagga aaagaaaaag
    10141 gaaatgttcc gagtttggca gaagaagtgc tcacagaatt ataagaatca ctagaacttg
    10201 tttggattat gaagagaaaa ttctgtgaaa gctgttgaaa cgatgacaaa atcaaccact
    10261 aggcagacat aagctatact tttatttttc cctatgatag aatacttgcg acttagactt
    10321 ttcgaatggg tgagaggatg gaattgattg aaaaagcgcc agccaagata aatttaggac
    10381 tggatgtatt atatagacgg caagatgggt atcatgagtt ggaaatggtg atgtcgagcg
    10441 tagacttggc agatcatcta ttttttgaag aaataacaga agacaaaatc attgtagaaa
    10501 caaataaagc atttttaccg gtagatgaac ggaacaatgt ttatcaagct gctttattgc
    10561 taaaaaataa atacaatatc aaaaccggtg tcaagatctt tgttaagaag aatattccag
    10621 tagcagctgg attaggcgga ggaagtacag actgtgcagc tgctctaaga gggatcaacc
    10681 aattatggaa tctgggctgt tcgttagaag aactggcaga aatcggtttg gaagtcggta
    10741 cagatgttcc ttactgtgtc tacggaagta cggcatttgt cggtggcaaa ggggaagaaa
    10801 tccaagtatt accttctatg ccacagtgct gggttgtgtt ggtcaaacca agaatgagtg
    10861 tctctacacg tacagtgttt agagaagtag atacgcaagc catccaacat caagacatcc
    10921 acggacttgc tgaagccatc cgagagcaag attatcagca gatgatcaaa aaaatgggaa
    10981 acagtttaga agatataacg ggaaagcggc atccagtcat ccatcaaatc aaagagcgta
    11041 tgatcaagta cggagcagat gctgcattga tgagtggaag cggaccaacc gtttttgcct
    11101 tgtgtcaaca ttattcaaga gcgcagagag tatataatgg gttaaaaggt ttttgtgagg
    11161 aagtttattt agtacgaacc ctgcaataaa atataagaag taaaataggt gatgaaaaac
    11221 tgaggaatcc taaaatggta ttcttcagtt ttttattgta gtgaaatggc tagatatgag
    11281 cagcaactca tccattttac gatttaatca agaatataca tgcttaaatc agaaattgat
    11341 ggatttctat tgactgtgtt tttttatttt gatacattag ttcacgttaa taaatcgtaa
    11401 aattccgatt tgttcattgg aaggagcata taaatgaaaa aatatctttt gacagctacg
    11461 gtaatgatag gtgctttact gtttgcagca tgtggaaata cgaacaagga agcagacaaa
    11521 aaagaggatt tgacgatcgt tacgactttt tatccgatgt atgattttac aaaagagatc
    11581 gtaggggatg aaggaaacgt gaaacttttg atccctgctg gaacggagcc gcatgatttc
    11641 gaaccaagtg caaaagaaag agcagaaatt tccgatgcag atgttttcgt gtataatagt
    11701 tcagacatgg aattttttgt ggattcgtta aaggattcag tggatagcaa acagaccttg
    11761 atgatcgaag cagctaaagg aatcgaccgt ttagaatcgc aagaagcaga cgaacatgaa
    11821 gaatcagaag aagggcatgg tcattcacat gaatacgatc cacatgtttg gctcgatccg
    11881 gtattagcta taaaagaagt cagaacgatt gctgaagaat taggcgaaaa atatccagat
    11941 aaaaaggaaa cttttacgaa gaatgccgat gcttatatca aaaagcttga agcactggat
    12001 caaaaatact ccgatgagct aaaagatgca acaaaccgca catttgtcac tcagcatgct
    12061 gcttttgctt atttagcgaa tcaatacgac ttaaaacaag tagcgatttc gggcgtttca
    12121 ccagatcagg aacccacacc aagtcgctta gctgagctaa aagaatttgt gaagaaaaac
    12181 aacatcaaag tgatttattt tgaagaaaat gcctcttcga aggtagctga gacattgtcg
    12241 aatgaaacag gagtgaagct agaagtgttg aatcctttag aaagcttgac taatgaacaa
    12301 atcaaagcag gcgaaaatta tatttctgtc atggaaaaaa atctgaaagc tttaaaagaa
    12361 agtatcaatt aagttttaga atggaggttg taaggtgcgt tatatcgaaa tatctgattt
    12421 gaccttttac tatgatgatg aacctgtact agaagatgtc tcttatcatg tagatgcggg
    12481 tgaatttgtc attttaactg gagaaaatgg agcagccaag tcaaccttgg tgaaagcatc
    12541 gttaggatta ctgaaacctt caaaaggaat gataaagatt gctgagaaaa atcaagaagg
    12601 aaaaaaatta agtatcggtt atattcctca acaagttgca tctttcaatg caggttttcc
    12661 tagtacggtg atcgaattag tccgttcagg aagatatcca agaggccgct ggttcaagcg
    12721 tctctctgaa aaagatcatc aacatgtaca gcgtgcttta gaagctgtag ggatgtggga
    12781 tatgcgaaat cggcgaatag gtgaattgtc tggcggacaa aagcagcgaa tcagtttagc
    12841 gcgtattttt gctactgacc ctgacttatt tgttcttgat gaaccaacaa caggtatgga
    12901 tgaagaatcc agaaatgaat tttatcgtct tctccgacat agtgcgcatg tccatgggaa
    12961 atcagttcta atgatcaccc atgatcatga agatatcaaa cagtatgcgg acagacagat
    13021 ccgactggtg agaaaggagg attctcaatg gcgttgcttt catatgaatt catgagaaga
    13081 gcgtttcttg ccgctatctt cattgccgga atcgccccga tgctaggtgt gtttttagtg
    13141 atcagaagac aatcgctgat ggctgatacg ctttctcatg tctctttagc aggggtagcg
    13201 ttaggattct ttttgaacct taatcctaca cttacgacca tgctagtcgt tgtattagca
    13261 gctgtgatat tggagtatct gcgaacgatc taccgaacct attcagaaat ctccattgcc
    13321 attttaatgg ctggtggttt ggcgctagcg ttagtattga tgaatttgag cggagggaat
    13381 tcagcaacaa gtatccaatc ctatctgttt ggttcgatcg tgaccattac atgggagcaa
    13441 gtcatctttt taggaatctt attcgttgcc atcggtatat tgttcttttt attcaaacgg
    13501 ccgatgtatg ttctgacttt tgatgaagat accgcccacg tggatgggct acccgttcat
    13561 ttgatgtcga tgatgttcaa tgtgattact ggtgtggcta ttgctgtgat gatcccgatt
    13621 gccggtgcac ttttgatatc cgcaatcatg gttcttcccg cagcaatcgg tatgcgatta
    13681 ggcaaaagct tcaatgtcgt gattgctatc agtgtttgtg taggatttct agggatgctg
    13741 tcaggactaa ctagctcgtt ttatctagat acacctccag gagcaacaat cacattagtc
    13801 tttattgctt tgttcctgat tgtaaatatc atcaaacgaa tattggttgc cttcagaaga
    13861 aaaaataact aaattataaa gaaaagaagc cttataaaaa cgaaaaattt tttataggct
    13921 tctttttatt ttaataaata ccgaactatt ttcttatttt ttcataaaat gctcgaattt
    13981 ttgaagaaaa acttatataa tgtaaaacga taaaatttct taaaaggagt tggaataggt
    14041 gaaagtacgt cgcagtgaac gtctagttga catgacacag tatttattga atcacccgca
    14101 tgaattgatt tccttaacaa gctttgccga acgttatgaa tctgctaaat cttctatcag
    14161 tgaagatttg ggaatcatca agaaaacatt taaagaacga gggtatggaa ccttggaaac
    14221 aatcccgggt gctgcaggtg gtgtattgtt catccctgaa atttcatatg aagaggcgaa
    14281 aaaatatatt gaatctctag cagaacgtct atcagaacaa gaccgtctgc ttccaggagg
    14341 atatgtttat ctctctgatt tgttaggaga acctgaactg ctgcgtcaag ttggaagaat
    14401 cattgcttct aaataccttg gtgaaaaaat cgatgctgta atgactgtag ccacaaaagg
    14461 cgtacctatt gcccaagctg tgtcctatta tctgaatgcg ccattcgtta ttgtacgccg
    14521 ggactcaaaa ataacagaag gatcaacagt aagtgtcaac tatgtgtctg gatcttctga
    14581 aagaattgaa aagatggaac tgtctaagag aagtttgaaa cgtggttcac gtgtccttgt
    14641 agtggatgat ttcatgaaag gcggcggaac agtcaacgga atgaagagct tgattgaaga
    14701 attcgaatca gaacttgttg gcatcactgt atttgccgaa tcaaaattca acggtcatcg
    14761 cgccatcgat gattatacgt cactgcttta tgtcaaagat gtagatactc aaacgaaaaa
    14821 tattacggtt gtgccaggaa attatttttc agaataagag gttgtgaaaa agccgcttag
    14881 tttcgagtag taagaaaaat tttgtgaaaa tggctttcca cattttttca taaatttggc
    14941 ttaataccga gaaactggct tttgaacacc gtttatctag aggttgtgaa atcagcgtcc
    15001 tgtcctgagt attagctcaa taaacggaaa aattgctcct catattttgc cgtttattga
    15061 gctaataccg aaggtcagct gattgaaaaa cgtttaatga agaggttgta agcctaccgt
    15121 ttaactctaa gtcacaagaa acgatttcaa aaaacagctt ttcatgtttt atgagtagtg
    15181 tgacttgtga ccgaggagtt ggcaggagag caccggttac caaatagata ggcccgttat
    15241 ggtctatcta tttttttatc cagaaaacgc tgggataaaa gaaaatcact tgttttgttg
    15301 aaaaaatggg tatactggga taaactgaaa tcagcaaact atatttagga gtgacgcagt
    15361 tggacgcacg ttatgcaatt attttggcgg cagggaaagg tacccgcatg aagtcaaaat
    15421 tatataaagt tttacatccc gtttctggta aacctatggt agaacatatc attaatcggg
    15481 tcagtgaaac gaaaccagat gaagtgatta cgatcgttgg acacggagca gaacaagtta
    15541 aagcacaact aggagaacgc agtaaatatg cgttacaagc agaacaattg ggaacaggac
    15601 atgctgtttt gcaagctgct tctttcttgg aaggaaaaaa aggaacaacg ttagttatca
    15661 gtggcgatac accattattg actactgaaa cgctgaacaa tctttttgaa tatcaccaag
    15721 gaaaaaatgc aagtgcaacg atcttgacgg cacaagcaga aaacccaaca ggatatggac
    15781 gaatcatccg tgaccatatt gggatcgtag aaaaaatcgt ggaacaaaaa gatgcaacac
    15841 cagaagaagc tcttgtacaa gaaatcaata caggaactta ctgttttgac aacgaagctt
    15901 tgttcgatgc gttaagcaaa gttggaacaa acaatgctca aggtgaatac tatctaacag
    15961 atatcatcga aatcttaaaa gaagaaggcc atacagttgc tgcttatcaa acggatgatt
    16021 ttgaagaatc aatgggagtc aatgatcgga tcgcgttagc taaagcaaat gagatcatga
    16081 gaaagcgaat taaccagatg catatggtca atggcgtaag ctttgtcgat tcagcaacga
    16141 catacatcga tgcaggcgta gaaatcggtc cagacacatt gatcgaagca ggtgtacaaa
    16201 tccaaggaaa tacagtcatt ggttctgatt gtgtcatcgg gtcacattca aaaatcgtag
    16261 acagtaggat tgaggatcac gtcgtcattg aaaactcagt cattgaaagc agccatgtca
    16321 agaaacatgc agatgtggga ccttatgcac atttacgacc aaaagctgaa attggcgaaa
    16381 acgtccatat cggaaacttt gtggaagtga aaaatgctca aatcggcaaa ggaactaaag
    16441 tcggtcactt gacttatgtt ggtgatgcta cgctgggaga agaaatcaat gtcggatgtg
    16501 gtgttgtctt cgtcaactat gatggaaaga acaaacatca tacgactgtc ggcgaccaca
    16561 gctttattgg ttctagcacg aatatcatcg gacctgtcga ggtagctaaa aacagttcga
    16621 ttgctgccgg gtcaacaatt acagacaata ttccagaata tgcgctggca attgcgagag
    16681 caagacaagt caataaagaa ggatatgcaa aaaaactccc ttatctaaac taagactttt
    16741 gcaaaatcac ttgatttatt taaaaaatat gactactatt tattaggtag aattctagag
    16801 ttgtgaaaaa gaaagtggag gtccccatgt caaaacatta ctttgaccca agattaaaaa
    16861 tttttgcgtt aaattccaat cgcccattag cagagaaaat tgcagcagca gtcggcgtcg
    16921 agttaggaaa atcatctgtt actcaattta gtgatggaga aattcaagta aacattgaag
    16981 aaagtattcg tggatcgcat gtctatgtta ttcaatcaac gagcagccca gtcaatgata
    17041 acttgatgga gttgctgatc atgattgacg cactgaaacg agcaagtgca aaaacaatca
    17101 acgtagtcat gccttattat ggctatgcgc gtcaagaccg taaagcacgt gcacgggaac
    17161 caatcacagc gaaattagta gcaaacatga ttcaaaaagc tggtgcaaca agaatgctga
    17221 ctttggacct tcatgcagtc caaatccaag gtttctttga tattccagtc gatcatttga
    17281 tgggtgcgcc acttattgcc aattatttct tagaaaaagg aatcaaaggc gatgatgttg
    17341 tcgttgtttc accagaccat ggtggtgtca cacgggcacg taaattagcg gaatttttga
    17401 aatctccgat tgcgatcata gacaaacgcc gtccaaaagc aaatgttgca gaagtaatga
    17461 atatcattgg acaagtcaaa ggcaaaactt gtgtactgat cgatgatatg atcgatacag
    17521 caggtacgat cacattggct gccaatgctt tgaaagaagc tggtgcaact agtgtgtatg
    17581 cttcatgtac acatccagta ttatccggcc ctgctttaca aagaatagaa gattcagcta
    17641 tcgagcgtct agttgttaca gactcgatct atcttccaga agatcgtaaa atcgacaaaa
    17701 tcgatgaagt aagtgttggc ggactaatgg gagatgcaat caaacgtatc catgaaaaca
    17761 aaccagttag tccattgttt gaaacaaaaa ataaataata aataaactca caaaggggag
    17821 tccaattggg ctgagattga atctatttct aaacccttcg tacctgtatc ggttatgcga
    17881 gcgtaggaat tgtgaataaa cagctgtttg actattttcg aaagctgctt cctcctttgt
    17941 gactttttta ttcaaaggag gaagtttttt tatgggaaaa aatagagttt gggtagaagg
    18001 aacgattgtt gcagcgttag cgatggtgtt gtcgttgatt ccgatccaga tcggcagcag
    18061 tttttctatt tcattagggc agattccatt gactcttttt gcgttaagaa gagggacaaa
    18121 ggcagggatg ctggcaggtt tcatttgggg catcctacat tttccgttgg gacaagttta
    18181 ttatttaagt gtcgttcaag tgttgattga gtacccgatt gcctatactt ttgcaggcgc
    18241 agcagggctc atggcttcaa atgtccaaaa aggctatgta gacgataata cgaaacaaat
    18301 tagaaaaagc atcgtgtttg gtgcattgat tggcgcactt tccagatatt tttggcattt
    18361 tattgcagga gtggttttct ggggagcgta tgcactatgg gggatgaatt cttggctctt
    18421 ttctttcgta atgaatggtg ccagcgggtt gagtacagct attgtaacga tcatcgtttt
    18481 attagggatc caacaaacga ctccttcgat ttttgtgcca gataaacgac aggagctgta
    18541 aataaaaaag aagcaagaaa cttgagacag aatcatgtcc tagtttcttg tttccttttt
    18601 attttttgat aagaaataga tctacttcaa tggcagataa gaaagatcgg catcttctac
    18661 caattgccag tgtcgatcgg tgacagagta gattaaagta gcgatgattt cctcttctcg
    18721 ataccgggtg ccttttaatt tccaaatata atcttcaatc acatagattg ggatacgata
    18781 tttaccatcg atcaaaccaa cttgatcaaa agaaaaaatg atcccttcct gcatcaaatt
    18841 acgcatttct ttcaataatt ccacactgat tttttcaaca ggtgtttctt ttttgaaaaa
    18901 agattttttt acattgatct tatctgacaa gatcgaactt aatttactag caaaaatcct
    18961 ataaaattca tctaaatagc gataataaat ccgattcagc tgatcattaa gatcgaagta
    19021 tacaaaatta ttctgtagtt tataaaaaaa cggcgaatga agatgtgttt tcatatgacc
    19081 aaaatataat aattctgaaa tttctaaagg tgtcaattct ttcagcatcg ttaaatcagt
    19141 aaagtcgatc cacttcaatt catcgaatcc acgcctgtta cgagaagagg aaaagaaacg
    19201 ctgaatattt tctgtaccac ggattacttt cagccctgta tgggattcat attctccagc
    19261 agaagcagat ggatcaagca agagcaagtt ttgaggaaaa tgcacgatac tgtttgcaaa
    19321 gtcagtagca gtcagccctt ttgttaaaac agcattactt gtggtatcaa tatgtacata
    19381 aactgactca gtcatcatgt tcctcctctt atttcctctt aatctctttt attttacaac
    19441 atttgcatta aaaaagcttg tttcttttat tttaaaattc cattacaaaa tcacgttttc
    19501 attgaaaaca ctctcatttt caattaattt ttgtgatttt tgtatttttt atttaaagga
    19561 tgttttatga attgttttct tatggaatct acaaagtcga atacgagact catgaccttt
    19621 ttatgacgta ctggtatact tgaagaaaaa ggagagcgcg atggagataa aaaaagttta
    19681 cttttcacag aaaatgtcgc tgtattcgcc tatccagcaa gcaaaacaac cccttccatt
    19741 ttttcaaagt catcaagaaa gtagagacgc tttttggcgt tctgcaaaaa agcaatgtgc
    19801 ttttaaagca gtatcgaaaa aatataccgt ctattttact attgctaaaa gtagaggaga
    19861 tgttattttt aataatctgt tgattgaagg tgaaactttt gaatggaaaa aattttttcg
    19921 gtatatggaa gcttgcgctc gtttttttat taaagaaaac tgttgttttt tatttcaatc
    19981 tcctctatca gaagaatggt tgcgtatttt tcataaaaac ggctatcaag gaaccacaca
    20041 attaaataaa aagctggtct atcatacagc acttgtttta ggtggaggag gagcacacgg
    20101 tgcttatcag attggtgtgt ggcaggcgct gaaggaacat gatatcaact ttgagattat
    20161 cacaggtacc tcggtaggtg cgttgaacgg cgcattgatt ttacaaggag atatgaaaaa
    20221 agctgtgaat ttatggaaaa agctgtcgac aagacaagtg cttgcgttac cagaaatggc
    20281 tgcagttgaa gaccttcgtg aacgcttcta tcgagaaaga cgccaaatga cgaaaacagc
    20341 actaattgaa ggcggggttt cttctgctcc attggaaaag atggtcaaag acaatttgga
    20401 tttgtcctta ctcaaacata atcccaaaat acgtttatat acggtgtcta ctaaactgcc
    20461 agaactagcg gaagtagtga ctgatatcca acagacaaaa acagaagaaa taccagattg
    20521 gatactcgcc tctgcttcat tttatcccgc tatggcttat cgaaagatcg gaaaaaataa
    20581 atatgcagac ggtgggtatc gcaacaaaat acctattgat attgcgatta acaaaggagc
    20641 aacagaggca tttgtcgtag atgtgcaagg gccaggccca gcgaaaaaaa ttcgtatgcc
    20701 agacattttt atccattgga aatgtcaaac attgtggaca ctaggcagct ttctgctttt
    20761 tgatagccaa aggaaccaat taaatttaca attaggctat ctggaaatga aaaaacgatt
    20821 aggctattac tatgggaact ggtatacatt tcattctgtt aaacctgcaa gaacatactg
    20881 gcgaggattt ttgtcttact taacaaaaga aattcagtta gaattatctt ttttcaaaag
    20941 cataaaattt tggcaaaaac tgcgtaattt atataagaac agggtcgtac cagagacatg
    21001 cggattggcg atgctagaac ttttagcaaa aaaacatttt ttactgccca atgaaatcta
    21061 tcaagtagac atgatgattc aaatgatctg ccaacaagat ataaaaagta tgccggatga
    21121 tttattggca caaattggtc aattaagtac tgaagaatgg cgaaggtatc gaaaatacca
    21181 gcaaaaaata caaaatgagc gcacgaaaac tgtttatttt gattatctgc tacaaggaaa
    21241 acgcaaggat cgtttgcagc atgaattgct taagcaacca gttgacacat tactgatcct
    21301 atatttatat tatctcaagg aggaacaacc atggcacaag aattttcata tgaaatcgta
    21361 gaagaaatcg ctatattgtc agaaaacaac aaggggtggc gtaaagaact gaacctagtc
    21421 agctggaatg gtcgcccgcc aaaattcgat cttcgagatt gggcgccaga ccatgaaaaa
    21481 atggggaaag gcctgacatt gacgaacgaa gagtttgaac aactgcaaaa agcaatagaa
    21541 aatatgtaat gaggaaggac cctgtcgaat gaaaagtatg actggttttg gcaaagccat
    21601 ccgcgaaaca aaagcgtatc agttagatat tgaaataaaa agtgtcaacc agcgttttct
    21661 agatgttcaa atacgaagtc ctaaaatcct gaactttgtt gaaaacgatt ttcgtcagct
    21721 tattaaacag cagttaagcc gcggtcgaat cgaagtattt gtgaatctag cttatattgg
    21781 caaaaacgaa aaacagatcg tcatcaactg ggaattgatt gattcactta tcactcaatt
    21841 gacagacgga attcaacaac gatacggtag tcaaaatcaa gtgtcaatta gtcggatggc
    21901 ggaacgcttc gcgctggacg aaaacttcgt aacggtagaa gaaaaaccgg aagagagcat
    21961 ggaagaattg gcacagttag ctctagatac attgaaaagc gcacttgatt ctattgaggc
    22021 gagtcgagaa aaagaaggtc aagctttagc tttagtgttg aaaaagaata ctgaagaatt
    22081 caaagaagta ctaaaaaaac tcgatagttt tgtagaaatc tatgaaaagg aatttcaaat
    22141 aaggtatcaa aaaaagctgg aggaatttct tggagcgaaa gtcgatgagc aacggttgtt
    22201 gacggagcta gccatcttgt tggaacgcgg agatattcac gaggaactgg atcgtctggc
    22261 gatccatgta caaaaactag atgaacttat cgaagcacaa tgtcctgtag gtcgggaatt
    22321 agatttcctg atccaagaaa tgaatcggga aatcaacacg atcggttcca aatcaaatgc
    22381 aatcgaaatt aaaaatcaag tcgtccagtc taagacgatc ttagaaaaaa tcagagaaca
    22441 agtacaaaat attgaatgac gaaagaggta gagataatga aatattatga cgtaacgttc
    22501 cacgaattaa gtggaaaaac tgtggttaaa cgggatatcc cttctgaaaa aaacggcttt
    22561 gacgtatgga aagatgcctg tgcagactat aatgagaacg aactgtttat tttgatcaac
    22621 gacggtgctt acgtgacgat gaatcggaaa tttattgttc gaattgacac agaagaggta
    22681 gaagatccga cagaaaaagc acgttctcga aaagatgaga tcatgggtgt agtgaatact
    22741 ttgtccaata tgggctttta gcaaggatgc tgaacaaaga aatacaataa ataatcagaa
    22801 ttggatttct tcgcttagct tctttgctct aggaatacat ttaaaggttg tgacaaaatt
    22861 cgtgtcacaa tcttttttta ttttgaataa gtgatgttca aaaaccattt tctcggtatt
    22921 aagccaaatt tatgcaaaaa tgtgagaaac tattttcaca aaatttttct tcatactcga
    22981 aactaatcgg cttcttcacg acctcttata cacggcgttc aaaagcttgc atctgcgata
    23041 ataaccccaa ccaaacgaaa aacatgagaa gctgtttatg tttggctgtt cttattactt
    23101 gatgcaggac agctttttta cgacctcttg gtacagggta gcttttttac caatttctag
    23161 ctataattaa agataacctt gaaagttgca gaaaaaaagt gcaaaataaa cgataagtac
    23221 aaaaagacta gttgaaagga cgtcgtcatg tcagagcgtg gattattaat cgtgttgtct
    23281 ggtccatccg gtgtaggaaa aggcacagta cgaaaagcaa tttttgagag tgaggataat
    23341 gattttcagt attcgatctc aatgactacc cgtaaaatgc gtgaagggga ggtcgaaggc
    23401 gttgattatt tcttccgaag caaagaagag tttgaatcaa tgatcgaagc tggagagatg
    23461 ctggaatacg cagaatatgt cgggaattat tatggaactc ctttgtcata tgtcaacaag
    23521 acattggatg aaggaaaaga tgttttcttg gagattgaag tccaaggagc aaaacaagtc
    23581 aaagaaaaag tgcccgatgg tgtattcatc tttttgactc cgccagattt agcggaatta
    23641 aaatcacgga tcgtaggccg aggaacagat gcagatgaag taatcgaaga acgaatgaaa
    23701 gttgcaaaag aagaaatcga aatgatggct ctttacgact atgctgttgt aaatgatgaa
    23761 gtgccgctag cagttgaacg aatcaaaaac attattgcca gtgaacattt tcgagtagat
    23821 cgcgtaatcg gtaaatatat taaaatgtta aaggagatgt agtcttatga tgttaaaacc
    23881 ttcaattgac tcactattag atcgtgtaaa ttcaaaatat tctctagtga ttttagcaag
    23941 taaacgcgcg catgaattgg atgcaagtgc acagcctact ctagattctt ttgattcagt
    24001 aaaaagcgtc ggtcaagctt tagaagaaat cgatgcagga aatgtagtca atgatccaca
    24061 tcctgaatta aaacgtgaac gtttgaagat ggaagaagag gaacgtcaag cacaaaaaga
    24121 gcgtgaacaa cacgaattgg aatcacgtat ccgtgaagag caaaaaatgt aataaagaaa
    24181 aatagagcga acatctaaaa agaaaagcaa acccagctgt ttccttttca aacattcgcc
    24241 acttattgtc tggtagaaaa ggctgtgtct gaactttttg acacagttct tttttttctt
    24301 atctgctgtt aattttttgt taggatttgg cagaagataa gacaaatcca tcgatctcta
    24361 gtaaaatagg caaggaactt tttgtcgctt gataagaaaa gacaaaaaaa ggaggaaacg
    24421 aaccatgaca ttaacagctg aagtcattgt ggacgtacct actatgcaga ccgatcagcc
    24481 attcacttat ttggttccct ctgaaatgga aacagctttg caagtcggga tgcgagtaga
    24541 agtccctttt ggtaatggca atcgtcatgt ccaaggattt gttatggcta tccaaaaatc
    24601 agaagaatca gctaatccat ctttgaaggc agttattcgt ttgctagacc ttgctccggt
    24661 ggtcaatgaa gaattacttt cattagctga ttatatgaaa aaaattactt acgcattcaa
    24721 aatcacttgt ctacaaacga tgcttccgag tgtgatgaaa gcagaatatg ataaattgat
    24781 ctatcctttg gcagacacac cagaagttca ggtattgttt gatcagcgag aagtgatttc
    24841 ctggaaggaa gcagaagaag aaggcagcct ttctcaattg attcgctggc gccaagagca
    24901 attagtagat atcaaatatg aagtccatac acgaaataaa gtaaaaacga ttcgtttggt
    24961 ccgttctttg ttaactgaaa agcaaatcga agaagaatgg gcaaaattac ggcaaaatgc
    25021 caagaaacaa aaagaacttc ttttgtgttt atctgaactg agccaagaag aaccaattgc
    25081 ttatttcaaa aataaaggaa tcagtactgc tgtcttgaat caaggaaaag aaaagggctg
    25141 gctggagttt atagaatcag aacgttatcg cgacccttat aaagaccgag tatttgacca
    25201 aacgacggca ttggaactga atgcagaaca aaaaaatgct gtcgaacaaa tcatcacagc
    25261 aggtcagcag caaaaagatc aggtcttttt gctggaggga atcactggca gcgggaaaac
    25321 agaagtatat cttcaggcaa tcgctgatgt tttgtcagaa aataaaacgg ctattatgtt
    25381 agtgcctgaa atcgcattga ctccgcaaat ggtcgagcga ttcaaaggac gcttcggaga
    25441 atcagttgcg gtcttacata gcggactttc ccaaggtgaa aaatatgatg aatggcgcaa
    25501 gattgagcgg gaagaagcgc aagtagtagt aggcgcacga tctgctattt ttgccccgct
    25561 gaaaaatatc gggctgatca ttatcgatga agaacatgaa tcaagttaca aacaagatga
    25621 gacaccgcgt tatcatgcga gagatctagc aatttggcgc agtaaatatc atcattgccc
    25681 aatagttcta ggaagcgcga cgccctcttt agaatcaaga gcacgagctc aaaaaggcgt
    25741 ttatcagtta ttgcagttaa accaccgagc caaagcagct gcacagcttc cagctattga
    25801 gatcgtagat atgcgtgaag agtttcagaa tcatcggacc tctacttttt ctgctaattt
    25861 gcaggaaaaa atacaaaatc gattagacaa aaaagaacaa actgtcctac tgctcaatcg
    25921 tcgaggatat tcgtcttttg tgatgtgtag ggactgtgga tttgttcttc catgtcctaa
    25981 ttgcgatatc tctcttacac tgcatatgga tacacgttca atgcgctgcc attattgcgg
    26041 acatgaagaa cctattccca atcgctgtcc taattgtggc ggcaataaaa tccgctacta
    26101 tggaacaggt actcaaaaag tagaagagga actccgagaa ctttatccgc aagctaggat
    26161 tttgcgtatg gatgtagata ccactcgaag aaaaggggca cacgaacaga ttttacaaaa
    26221 attcggtgca aaagaagcag atatattatt agggacacaa atgattgcta aaggtttgga
    26281 ctttccagaa gtgacattag ttggggtgtt gaatgctgat acctccctga atttaccgga
    26341 ttttcgttct agtgagcata cattccaatt gcttacccaa gtttcaggac gagcagggcg
    26401 agcggaaaaa gcaggcgaag tcgtgatcca aacattcaat cctcaacatt acgccataga
    26461 attggctaaa aaacaaaatt atgagcagtt ttatcagcag gaaatgcatg tacggcatcg
    26521 tggaggctat cctccttatt actttactgt gaaaataacc gccagccatc ctgaagaaca
    26581 agtagcggca aagaaaatct ttcagattgc caatcagcta aaagaagtgt taagccctca
    26641 aagcttgcta ttagggccaa cgcccagcat gattttgaga gtgaaaaacc gatattatta
    26701 tcaactaatc atcaaatata aacatgaacc gaatcttcct catgttttgg atgaaatttt
    26761 gaatggaagc cagaaagaac agcgacaagg tctttttgtg gcaattgaca acgaaccatt
    26821 aaactttatt taagaaaggg ccaaattatg cgctatccta tattgatcca ccctaatgat
    26881 aaattaaaaa gaactgctca gccaatcgat gtaatcacgg acgagacaat cgctttatta
    26941 gacaatttat atgagacaat gattgcgaat gacggaatag ggatcgctgc tcctcaagta
    27001 ggacaaaaca agagaattgc tgtgatcgaa gtagatgaag gcgagaaatt tgaactgatt
    27061 aacccagaaa tcatcgaagc aaaaggtgaa agccttgacg tagaaggttg tctcagtatt
    27121 ccgcatgtct atggaacggt caagcgagca gatgaagtga ccgtgcgtta ttatgatcga
    27181 gacggagaag agatagaagt gacggctttt ggctatttag cccgtgcctt ccagcatgaa
    27241 atcgatcact tagacggtat cttgttcatt gagaaaatga tacagcagat cccggaagaa
    27301 gaacttgaag aatatatgga ggaacatctt gatgactaag ctagtattta tgggcacacc
    27361 tgctttttct gtgccgattc ttgaagggct gatagaatca ggttacgaga tccaggcagt
    27421 ggtaacacag cccgaccgtc cagttgggcg gaaacgagta attacgccga caccggtaaa
    27481 agaagcggct ctgaagcacg gtatccgtgt gctgcagcca gagaaaatct ccggttcgcc
    27541 agaaatggaa gaaatcattg aattggtgcc tgatgtgatc gtcactgcgg catttggcca
    27601 attcttgcct gaaaagttat tgcaagtacc aaaattaggg gcaatcaatg tccatgcttc
    27661 tttacttcct aaatatcggg gaggtgcgcc tgttcattat gccatcatga atggggagaa
    27721 agaaactggt gtaacgatca tggaaatgat caaaaaaatg gatgcaggcg gaatctatgc
    27781 gcaagaaagc ataccaatca ctaaacaaga tgatgtaggg acgatgtttg aaaagcttag
    27841 ccttttagga agaaagctat tgctagaaac attgcccaat attttggatg gacaaaaacc
    27901 agtacctcaa gacgagtcag aagcaacttt ttcgccaaac atcacacggg aacaagaagc
    27961 aatcgattgg cataaaactg cagaagaaat tgacaaccaa gtacgcggaa tgcgtccttg
    28021 gccaattgca ttcacgacct atgagggcat acgctggaaa ttattatcgg ttgaagtatt
    28081 ggcagaaact acagacaaag cacctggtac gatcatccgg aaagacaaaa aaaatctatg
    28141 gattgcttgc ggtaaacaga ccgtcttagc catcaatgaa ttacagccag ctggtaaagg
    28201 aaaacaggcc gtacatgatt tccttaacgg aagcggtcag caagttcaga ttggtcagca
    28261 ggtgaaataa tgaaaaaaat ccctaaaaaa atgaaaaaat cagtacgata tatggcatta
    28321 ctgacattag agcgagtgaa caagggtggc gcctactcga atcttttgtt gaatgagatg
    28381 atcaaacaaa gcgagctaaa tcaaaaagat gtgggtttat ttacagaatt gatctatggt
    28441 acgattagcc atcagcgact attggaattt tacgttgcac cattgatcgc taaagcaaaa
    28501 aaagtggatg actgggtcaa gacgcttctt tatctttctg tataccaatt agaattttta
    28561 gataaagtcc ctgcacatgc tattttaaac gaggcggtcg aaatcgcaaa agtaaaagga
    28621 aatcctggga cagggaaatt tgtaaatggt gtcttgagga attatcagcg tcaaggagca
    28681 ccagatctgc aaggcattgc tgatcctatc gaaagacttt ctgtcaaaat cagcctgcct
    28741 ctatggatga ctaaacgact ggtggatcaa atcggatacg aagaaactga aaagctcggt
    28801 ttatcgctat atcagcctag ccatgctagt gcccgagtag atacacgccg actttccaga
    28861 gatcaggcca tcactgtttt gcaagaagaa gagatcacag ctgaaaaaag ccaagtcagt
    28921 ccatacggta tagtagcgaa aaaagggcat ttagcttcta gctcattgtt ccacgatggc
    28981 gtcatgacga tccaagatga aagttcgatg cttgtagcgc cgtctatgca aatcgagcct
    29041 gaacatcatg tcttagatgc ctgtgcagca cccggaggta agacgacgca tattgcttct
    29101 tttttagatc cgtcatgcgg tggagaagtg gtcgctttag atgtacacga acacaaagtc
    29161 aaactcattg aagataatgc agcacgtcta ggtgtctctt ctgctgtcta cgctcaaaag
    29221 atggatgcta gagaagtcag taaacagttc gaagcagagc agtttgatcg gattttagta
    29281 gacgcgcctt gttcaggtct tgggctgctt cggcgaaaac cagatattcg ttataaaaaa
    29341 cagccggatg aattagcgaa tttaccaaaa atccaattgg aaattttaga aagctgcgca
    29401 ccaactttaa agtcttccgg tattctgact tatagcactt gtacgatctt aaaagaagaa
    29461 aaccaagacg ttgtttctgc tttcttggaa agacaccctg attttgagag gattgatgtt
    29521 ttgaccaatc ctatgctaca atcatcaatc gaagagaaaa tgttgacttt gtatccgcat
    29581 cagttttaca cagatggatt tttcatttgt tgtttgcgca aaaagtaatg aggtgaaaaa
    29641 atgcagattg aatatcaatc agatgtagga agacgacgta acacaaatca agactatgcc
    29701 agtgtattta ccagccgttc aggtctcaag cttgctgtat tagcagacgg gatgggtggt
    29761 catcgtgccg gagacattgc cagtcagatg gcggtaacga atctaggcga agcttgggaa
    29821 aaacaagaag ctgttgacga tgaaaaaatc gcccaatggt tcattcaatc gattcaagaa
    29881 gaaaatatgc tgatttatca gcgtggacaa gaacagccgg aatataacgg tatgggaaca
    29941 acaatcgtag cggccgctct gtcagatgac tggttcacga ttgccaatgt tggggatagc
    30001 agagcttatt tgattcgtga agataaaatc atgcaattga cggaggatca ttctttagtc
    30061 aatgaactag tcaaatcagg agaaatcagc gaagaaatgg cagtgaacca tccaaggaaa
    30121 aatattttaa cacgttctgt cggcatgcct ggaacagtcg aagtagatgt ttctactcat
    30181 ctttggcaac taaaagatcg actgctgtta tgttcagatg gcttgacgaa catgctgtct
    30241 gaagaagaaa taggaacgat catcaatcaa gaaggcccat tgtctcataa agtaactgag
    30301 ctgattgatc gtgccaatga agcaggcgga gcggataata tcaccgttct tcttatcgaa
    30361 tataaggagg acagtgtatg atcgagttgg gcaaaaaatt aaatggccgt tatcaaatca
    30421 ctggaaatat cggtagcggt ggtatggcaa atgtattttt ggcgcacgat ttaatcttgg
    30481 atcgtgatgt agcagttaaa gtcttacgat ttgactttca aaatgaccag actgcgattc
    30541 gacgatttca gagagaagct ttagcagcaa cagaactcgt tcatccaaac attgttagtg
    30601 tttacgatgt tggtgaagaa gacaacatgc agtatctggt gatggaatac gtcaaaggaa
    30661 tggacttgaa acgatatatc caaacacatt atcctgttcc ttatgaaaca gcagtaaata
    30721 ttatgcagca gatcctttct gctatttctt tagctcataa ccatcaaatc atccaccgag
    30781 atttgaaacc ccaaaacgta ctgatcgata atgaaggtgt tgtaaaaatc acggatttcg
    30841 ggattgctat cgctttatct gaaacatcga tcacgcagac gaatacgatg ctaggatctg
    30901 ttcattatct gtcccctgag caggccagag gaagtatggc aacgaagcag tcagatattt
    30961 atgctttagg gatcatcctt tatgagatgt tgacgggtag cgtaccgttt gatggtgaat
    31021 ctgctgtaac aattgcttta aaacattttc aagatgatct tccatctata aaagcacttg
    31081 atccaaatgt gccacaagca ctggaaaacg tgattttacg ggcaacggct aaagaacctg
    31141 cagaccgtta taagtcagcc gaggaaatgt ctgatgatct ttctacagta ttatcaccag
    31201 ctcgtgcaaa cgaagaaaaa tggcagcctc atgtgatgga taatgaaaca aaagttatca
    31261 caccccttac ggaagatgca ccaatgcctg attcattcaa atctatgcct ctgccaaaag
    31321 acaagccaga aaacgaaacc ttaccagagg aggaagaagt tctgcctgat cctaaaaaga
    31381 aaggacagaa aaaatggtgg ctgatcctat tagctattct tgctgtttta ggaatcggaa
    31441 cagctgtatt ctttgcttct ggcgggcgag gagaagttgt tgttcctgat gtttctgatc
    31501 tttctgaagc ttctgctaga gaaacactcg caaaagcgaa attagaagta gcaacaaaaa
    31561 cagaagaaat tgcagatgac aaaatcgaag aaggtaatgt agtgaagaca gacccggcag
    31621 cgggaacgac agtcaagcaa aaacgcgaag tcactctgta tattagtagc ggcacgaaaa
    31681 aaatcaaatt agatgattac agtggtaaaa gttatgaaga tgccgaagca agtttgaaag
    31741 aattagggtt ttctgctgac ttgattcaaa aagaagagga attcagttca gaggtagaaa
    31801 gcggaatgat catttcgcaa tctccatcag aagatacaga agtcgatcct aaaacagaca
    31861 cgattacttt cacagtcagc aaaggaccag aaacagtgac tttgagtagt catgtaggag
    31921 aaaactacga aactgttcgt aattggcttg ttacacaagg attcaatatc aataatatta
    31981 gtgctactta tgactatagc gacactgtac catcaggttc gatcatcagt caggatccag
    32041 cctcgggtga agttgtagca gaagaaactt acatcaattt tgtcgtaagc caaggaacag
    32101 agcctaaatt gaatgatatc tccggctata caaaaagcga agcacaaagt tatttagcta
    32161 gtgtcggtgc agaatatatc gggcatgaga cttccgaata ttcaaacacg gtggataaag
    32221 ataaagtgat tcgtacagta ccaggggcag gaacgacaat taccaaagga atggttgtga
    32281 acgtaattta ctctaaagga ccagatccag cgtcttctag ttctgaaaaa gactcttcag
    32341 acagttctag cagctcaagt agctccagta gcacaagcaa ctcttcaaaa gaagaatctt
    32401 ccagctccac aagttcatca gctagcacct cagaaagttc aaaataagtg ctgaataaaa
    32461 tatatcagaa gggaatgcga cattgatttg ttgcattctc ttttttgtgt ttctatctct
    32521 tttgatcaat tttcaagcca tatactttga gaagtttgca tggaataaca cctactttcc
    32581 tcttcgtatg gtaaaataaa caaaaacgga ggtggcactt atcaaaggac aaatcagaaa
    32641 agcaatcagc ggtttttact atgtatacgc aaacggagag acctatcaaa caagaggaag
    32701 aggaaatttc cggaatcgga aaatcacgcc gcttgtcgga gatcaggtga tctttgagag
    32761 tgaaaatcaa acagacggct atcttctgga aatcttgccg agaaaaaatc aattagttcg
    32821 cccaccagtc acaaatgttg atcttggaat cattgtcaca agtatcgtag agcctgcatt
    32881 ttcttataat ctacttgacc gctttctagt taccttggaa tacgagtcga tcgaaccgat
    32941 tatttttttg acaaagaccg acttgtcaga cgacccagaa aaaatcgaag agatcaagca
    33001 aacctatact aaaataggct atccggtgat tgtacccaat catacgggtg atacgacaga
    33061 actgatccgt tacttcccag aacggctatc ggtctttatg ggtcagtctg gagcaggaaa
    33121 atcgacgcta ctgaatcaaa tatcaccgga actgaattta gcaacaggag agatttctga
    33181 tgcacttgga agaggacggc atacaacacg tcatgtggaa ctgattccta tctatggcgg
    33241 gttagtagca gatacaccag gatttagctc gattgacttt ttgacaattg aagcaggaga
    33301 actgccgaga caatttcctg aatttgtaga agcttctgtc cattgcaaat ttagagagtg
    33361 catgcatgct aaagaacctg gctgcgaagt aaaacataga gtagaaacag gtggtatcgc
    33421 acagacacgt tacgacaatt atttgcaatt cttacaagaa atcgaaaaaa gaaaaccaat
    33481 gtatgcaaaa aaagaaggta aacgaaaata ggaggaaaca agaatgaaga tagcaccatc
    33541 cattttgagt gcagattttg ccaatttaca aagagacatt gagttagtgg aaaaaggagg
    33601 cgcagattac atccacgtag atgtaatgga tggccaattt gtcccaaata tcacattagg
    33661 gccaaatatt gttcaggcaa tccgtcctgt tactaaatta ccgttagacg tccatctgat
    33721 gatcgttgat ccagaaaact acattccagc atttgcaaaa gctggagcag atatcattac
    33781 tgtccacgtc gaagcaactc ctcatattca tcgggcactt caaatgacaa aaagcttagg
    33841 agttaaagct ggagtagtta tcaatccagg aacaccagtc tccatgatca aacatgtttt
    33901 gcctttggct gatcaagtac tagtcatgac tgtcaatcct gggttcggcg gacaaagctt
    33961 tattgaagaa acagtagaaa aaattacgga actcgctgat ctacgtagag agaacaattg
    34021 gacttatgca atcgaagtag atggcggaat cgttccagaa acagcaaaaa tctgtaaaga
    34081 tgcaggggcg gatgtcttcg ttgcaggatc atatatctat ggagccgaaa atccagtcgg
    34141 acaaatcgaa cgattaaaag aggcattaga ctaaatgaaa attttattag ctgctggagg
    34201 taatccgcaa aactggccgc agtctctgct gtccaaatac gatgcctatg tcgggataga
    34261 ccgtggtgcg ctgtacctgc ttcaagcagg ctacccagta gatctggcag tgggtgattt
    34321 tgattcattg tcgaacgaag agcagaagga agtatttgca aaagccaaag agtatacccg
    34381 ttcccctgca gaaaaagacg atacagatac acagttaggt atcttaaaaa catttgaacg
    34441 ttacccgcaa gcggaagtca ctttgattgg agctacgggc ggaagattgg atcatctttt
    34501 agccaatctc tggttaggta tggaacctcg cttcaaaccc tttgcccatc agcttattat
    34561 cagagattgt caaaattatt tgtcttatta tttgcctgga gagcatatag taagacagat
    34621 accagatatg aagtatttag cttattgctg cttgactccc gtctcgaatc ttacattaaa
    34681 aggcagcaag tatgtattgg aaaataaaga agtagaatat ccgacatctt atgcaagtaa
    34741 cgaatttctt tcagataccg cagaattttc tttttcagat ggtatgattg cagttattca
    34801 aagcaaagat tgatccataa acaaataaaa aaatttgtct ttcaggctaa aaatgacaga
    34861 atctattatt tgctaaaaaa aagcttaaga ggagcaactt accttttgtt tcacggcatt
    34921 tagtgataaa attgaacaca agagtctaat taattcgagg tgtctttgtg aagaagttca
    34981 atcctaataa aaatattatt attaccttgg ttattgttat catcgtggtg acgatactaa
    35041 gtgtgaccgc tgcgcagcga gcgaatgaag gaaagcccaa tttcttccaa tccatcgtta
    35101 atgacagtgt tgcaatggtt gataaaacaa tctctgcccc agtacggtgg gtacgaaatg
    35161 gagtagattc tgtccataat cttttttcaa cttatgccga aaatgagcgg ctaaaagaaa
    35221 aaatcgatag ctatgatgac gtttcacagc aaaacaagaa ttacgagaga gaaatcgaag
    35281 cgttgaaaaa agaacttgag ttgaatcaga cgctgactag ttacgaaaaa gtaacggcaa
    35341 atgtcattac tcgttcgcct gacagttggc aggacatgct gatcgtagac aagggttcta
    35401 aagacggtat cgaagcaaat atggcagtca tgtcgcaaaa aggattgatt ggccgagtga
    35461 tcgaggtcaa tacggcttcg tctaaaatcg aattactgtc atcctctaat gaaagctcca
    35521 atcattttcc agtacgggta tcttcggcta atggcgaagc gtttggtttg cttaaaaact
    35581 atgatgaaaa gctccatgcc ttagtggtga cccaattaac tggtgatacg gatatcaaag
    35641 aaggggatgt tgtccagaca tccggtcttg gagggaattc tccagctaac ttgccgatcg
    35701 gtacggttat taaaacgaaa ccagatagtt atgggctgga tcgggaagtt tatgtgaaac
    35761 cttatgcaga aatgtatgac gtgtcagttg tgacgattgt ccaacggcta gcggaggatt
    35821 agtccatgtt gaagaaagaa acaatgaagt actatctgcc aatcgttttg ttctttttga
    35881 tgttgataga tggtcattta acaagaatgc taggggagtg gtcgaaaggc acctatatgt
    35941 caaatgccca ctttctgata ttggcattat tatgttgcag tatggcgttt gaaaaacgtt
    36001 atttactgat taccacgatt gttctcgggg ctatctatga cgcttactat attggcgtta
    36061 tcggtatcta tgcagtagct ctccctttaa ttgtatggtt gatgtatgta atgaaagacg
    36121 ttatccatgt caacatcttt actgaatttt tcagtatgat catctttgtc acgggttatg
    36181 aattgtttac gatggtggtc cagttgattt ttaaattagc agtagtaaat aacacgtatt
    36241 ttattacaag gtttttagga cctacactgc tgttgaacat gattatattt gtattattca
    36301 tttttccctt taagaaatta ttcagtgatg aataatttct ttttttattt ttaggtcgaa
    36361 agcgaaaaag aatcgttgtt atcttaagaa atgctagact taacgcggct ttttcatgat
    36421 cattttctga aacgaacagt tttgtcattt ttaaaagttg ttttgaaaca tttttgtaac
    36481 ataactatta tatcgttgac acactgctat ggtacaattt ttatatcgtt aagaaagact
    36541 taaatatatt tagatattta ttaaattaag ccagtcggag gaatgaagag tgaaaaagag
    36601 tttaatatca gcagtaatgg tatgttcaat gaccttgact gccgtagcat caccgatcgc
    36661 tgccgcggcg gacgattttg attctcagat acaacagcaa gatcaaaaga ttgcagactt
    36721 gaaaaatcaa caagcagatg cacaatcaca aatcgatgcc ttagaaagcc aagtttctga
    36781 aatcaacaca caagcacaag acctattagc aaaacaagat acattgcgtc aagaatctgc
    36841 acaattagta aaagacattg cagatttaca agaacgtatt gaaaaacgtg aagatacaat
    36901 ccaaaaacaa gcacgcgaag cacaagtaag caacacaagc tcaaactaca tcgatgctgt
    36961 attgaatgct gattcattag cagatgctat tggacgcgtt caagcaatga caacgatggt
    37021 aaaagcaaac aacgacttga tggaacaaca aaaacaagat aaaaaagctg tagaagacaa
    37081 aaaagctgaa aacgatgcta aactaaaaga attagctgaa aaccaagctg ctttggaatc
    37141 tcaaaaaggc gatcttttat ctaaacaagc tgatttgaac gtactgaaaa cttcattagc
    37201 tgctgaacaa gcaactgctg aagacaagaa agctgacttg aaccgtcaaa aagcagaagc
    37261 tgaagcagag caagctcgta ttcgtgaaca acaacgtcta gcagaacaag ctcgtcaaca
    37321 agctgcacaa gaaaaagctg aaaaagaagc acgcgaacaa gcagaggctg aagcacaagc
    37381 tactcaagct tcatctactg cacaaagctc agcaacagaa gaatcatcag ctacacaatc
    37441 ttcaatgact gaagaatcat catcagctac acaatcttca gcaacagaag aatcaacaac
    37501 acctgaatct tcaacagaag aatcaacagc gcctgaatct tcagcaacag aagaatcaac
    37561 aacagtgcct gaatcttcag caacagaaga atcaaaaaca gtgcctgaat cttcaacaac
    37621 agaagaatca acaacacctg cgcctacaac accatcaaca gatcaaagtg ttgatacagg
    37681 aaacggcaca ggaagtagta ctccggctcc aacgccaaca ccaacacctg aacaaccaaa
    37741 acctgtaaca cctgctccag ctccttctgg aagtgtgaat ggtgcagcaa tcgtagcaga
    37801 agcttacaaa tatattggta ctccatatgt ttggggcggt aaagatccaa gtggctttga
    37861 ctgctcaggg ttcacacgct atgtttacat gcaagtaact ggtcgtgaca tcggtggttg
    37921 gacagttcct caagaatcag ctggaacaaa aatttcagtt tctcaagcaa aagctggaga
    37981 cttgttattc tgggggtcac aaggcggaac ttaccacgta gcgattgctt taggcggagg
    38041 acaatatatc catgctcctc aaccaggcga aagcgtaaaa gttggttcag ttcaatggtt
    38101 tgcccctgac tttgctgtca gcatgtaaga atgattaatc aaaaagagtt agcccattct
    38161 ggactaactc tttttt
//
LOCUS       DACEUZ010000009        37222 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.009, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000009 DACEUZ010000000
VERSION     DACEUZ010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37222
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.009"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            147..1016
                     /locus_tag="IXG44_000412"
     CDS             147..1016
                     /locus_tag="IXG44_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAQ1657154.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIISSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            1225..2250
                     /locus_tag="IXG44_000413"
     CDS             1225..2250
                     /locus_tag="IXG44_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAQ1657155.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            2289..3239
                     /gene="manA"
                     /locus_tag="IXG44_000414"
     CDS             2289..3239
                     /gene="manA"
                     /locus_tag="IXG44_000414"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HAQ1657156.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIASEPGK"
     gene            3333..5036
                     /locus_tag="IXG44_000415"
     CDS             3333..5036
                     /locus_tag="IXG44_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1657157.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSIIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKTSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            5038..7071
                     /locus_tag="IXG44_000416"
     CDS             5038..7071
                     /locus_tag="IXG44_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ1657158.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRCVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            7082..7522
                     /locus_tag="IXG44_000417"
     CDS             7082..7522
                     /locus_tag="IXG44_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ1657159.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAETIEEVQMIFEHGHI"
     gene            complement(7722..8849)
                     /locus_tag="IXG44_000418"
     CDS             complement(7722..8849)
                     /locus_tag="IXG44_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1657160.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLSLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(8852..9529)
                     /locus_tag="IXG44_000419"
     CDS             complement(8852..9529)
                     /locus_tag="IXG44_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1657161.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            9767..11560
                     /locus_tag="IXG44_000420"
     CDS             9767..11560
                     /locus_tag="IXG44_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HAQ1657162.1"
                     /translation="MKRREGETKWNGRFPKNNISLKARVQKLGSTLSSMVMPNIGALI
                     AWGVLTALFIPDGYLPNEAFATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATM
                     GVIVGTDIPMMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLAL
                     LGFSAIGPVVDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLG
                     TEQVAEAGKSILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYF
                     PYVMMKPLLFLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILG
                     VIVAAAVSFGISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQK
                     IIFACDAGMGSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERA
                     KQKAPSARFVSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEI
                     LLVHDDRSGSATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQ
                     MAAPQSIHLSVNSMVNPQKYESIAQYLKQTA"
     gene            11575..12012
                     /locus_tag="IXG44_000421"
     CDS             11575..12012
                     /locus_tag="IXG44_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ1657163.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            12025..13176
                     /locus_tag="IXG44_000422"
     CDS             12025..13176
                     /locus_tag="IXG44_000422"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAQ1657164.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(13238..13936)
                     /locus_tag="IXG44_000423"
     CDS             complement(13238..13936)
                     /locus_tag="IXG44_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobyric acid synthase"
                     /protein_id="HAQ1657165.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(13929..15281)
                     /locus_tag="IXG44_000424"
     CDS             complement(13929..15281)
                     /locus_tag="IXG44_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HAQ1657166.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(15504..16904)
                     /locus_tag="IXG44_000425"
     CDS             complement(15504..16904)
                     /locus_tag="IXG44_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HAQ1657167.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            17065..17802
                     /locus_tag="IXG44_000426"
     CDS             17065..17802
                     /locus_tag="IXG44_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HAQ1657168.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            17927..18406
                     /locus_tag="IXG44_000427"
     CDS             17927..18406
                     /locus_tag="IXG44_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HAQ1657169.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            18403..19170
                     /locus_tag="IXG44_000428"
     CDS             18403..19170
                     /locus_tag="IXG44_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1657170.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            19400..19969
                     /gene="lepB"
                     /locus_tag="IXG44_000429"
     CDS             19400..19969
                     /gene="lepB"
                     /locus_tag="IXG44_000429"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAQ1657171.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(20073..20675)
                     /locus_tag="IXG44_000430"
     CDS             complement(20073..20675)
                     /locus_tag="IXG44_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAQ1657172.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            20781..21250
                     /locus_tag="IXG44_000431"
                     /pseudo
     CDS             20781..21250
                     /locus_tag="IXG44_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            21356..21940
                     /locus_tag="IXG44_000432"
     CDS             21356..21940
                     /locus_tag="IXG44_000432"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAQ1657173.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            21943..23016
                     /gene="prfA"
                     /locus_tag="IXG44_000433"
     CDS             21943..23016
                     /gene="prfA"
                     /locus_tag="IXG44_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HAQ1657174.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            23009..23848
                     /gene="prmC"
                     /locus_tag="IXG44_000434"
     CDS             23009..23848
                     /gene="prmC"
                     /locus_tag="IXG44_000434"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAQ1657175.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            23841..24020
                     /locus_tag="IXG44_000435"
     CDS             23841..24020
                     /locus_tag="IXG44_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1657176.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            24042..25067
                     /locus_tag="IXG44_000436"
     CDS             24042..25067
                     /locus_tag="IXG44_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HAQ1657177.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            25242..26486
                     /locus_tag="IXG44_000437"
     CDS             25242..26486
                     /locus_tag="IXG44_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HAQ1657178.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            26639..27586
                     /locus_tag="IXG44_000438"
     CDS             26639..27586
                     /locus_tag="IXG44_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ1657179.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            27720..28349
                     /gene="upp"
                     /locus_tag="IXG44_000439"
     CDS             27720..28349
                     /gene="upp"
                     /locus_tag="IXG44_000439"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HAQ1657180.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(28488..28730)
                     /locus_tag="IXG44_000440"
     CDS             complement(28488..28730)
                     /locus_tag="IXG44_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1657181.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            28851..28923
                     /locus_tag="IXG44_000441"
     tRNA            28851..28923
                     /locus_tag="IXG44_000441"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:28884..28886,aa:Thr,seq:cgt)
     gene            complement(29042..29680)
                     /locus_tag="IXG44_000442"
     CDS             complement(29042..29680)
                     /locus_tag="IXG44_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAQ1657182.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(29803..30435)
                     /locus_tag="IXG44_000443"
     CDS             complement(29803..30435)
                     /locus_tag="IXG44_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAQ1657183.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            30786..31085
                     /locus_tag="IXG44_000444"
     CDS             30786..31085
                     /locus_tag="IXG44_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAQ1657184.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
     gene            31580..33745
                     /gene="acm"
                     /locus_tag="IXG44_000445"
     CDS             31580..33745
                     /gene="acm"
                     /locus_tag="IXG44_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HAQ1657185.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(34017..34502)
                     /locus_tag="IXG44_000446"
     CDS             complement(34017..34502)
                     /locus_tag="IXG44_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HAQ1657186.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     gene            34982..35623
                     /locus_tag="IXG44_000447"
     CDS             34982..35623
                     /locus_tag="IXG44_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ1657187.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            35642..36277
                     /locus_tag="IXG44_000448"
     CDS             35642..36277
                     /locus_tag="IXG44_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ1657188.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            36295..37110
                     /locus_tag="IXG44_000449"
     CDS             36295..37110
                     /locus_tag="IXG44_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ1657189.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
BASE COUNT        12796 a         6381 c         7504 g        10541 t
ORIGIN      
        1 tttataagag tttttactca ttaatttaca aaaaacaagc aaaatgcgaa ttttctcaca
       61 ttttttttaa caaaatcaaa gttattgctt gaaagaagaa gaatccatcg ttaaaataaa
      121 gcttgacgta gaggggagca agaaatatga aagattttca attgggggct gccacctatt
      181 taaaaagtgt agccattcgg gtgaaagacc gagataaaat gattgatttt tacaagaacg
      241 ttgttggctt tgatttgaaa agagaagaaa acgaattagc cattttagga acagaagaag
      301 ctggaagtga aatgttgctg ctggaagaaa gtcctagagc gaatgatcac atgggggtga
      361 tcaaaaagct gaaccgtttt tcattgatta tctctagcac agaagaaatg gcagatatcc
      421 tttgtcgtat ccgtaaaatg aattatccaa tcaaagaagc tttagaagat catggacgta
      481 tggggatttt gctcgtagac ccagaagaga atgaattgga aatttaccat gaagagaata
      541 agaacgacca aagagaaatt ccagaaacaa tggatcaaga agcattatta agaaaatctg
      601 aagaaaaatt cagtaaacta tcaacaggtt cttattttga aaaaatccat ttaaatgtta
      661 ctaacttaac aaaagaacgc caatttctta atcatgtact tggtttgaag gtacataatg
      721 aagaagaggg catccatgta ttgaatgaag gagatttcca tgttggtttg acagaagcta
      781 gcggaggcgc aattgatttg cctacagatg aagtgattgg acttgatttt ctgaaattca
      841 tgatcgatac tgaaagcatg gaaggactga ttgctcactt gcagcaacta tcagttgatt
      901 tcttcttaga caaaaagcac acagtactca caatctatga tgctatagga atcgaatggt
      961 ggttcgtcag tgagccaaaa gaaaagaata aaactgacat tcgatgcgac gcttaacttt
     1021 ttctttctta acgattgtcg catgtctcgt cattttatga acggtcatac tatcgcttat
     1081 tctagagata gaaagagaaa atagtatacc gggggaaact gcgcggtttt tcgcgcagtt
     1141 tttttgtgta aaaaaggttg attcctcata gattaagctt taagcttgga ctgtaagaat
     1201 ctgatagaaa agcaggagaa aaagatgtat ccacacacga aacagcttat tttgaaaaag
     1261 atggaagaag gattttatcc aggtgtagca gtcgccttca taaaagaaaa tcaagtagaa
     1321 acatttacag ccggctttgc ccaagtcgtt cctgaaaagc agctgatgac agaagatctg
     1381 ttgtttgatg cagcctcact gacgaaagtc gttgcaacca cttcattagt tttacgtcta
     1441 ttagaagatg gaaagataga actcgatcaa ccgcttcata aatatcttcc tgaatttgaa
     1501 aatcagacaa tcactttgcg tcatttactg acacatactt ctgatatcaa aacatggata
     1561 ccaaacagag ataagctatc tcaggaagaa ttgatcgccg cttatttgaa gctagaacca
     1621 ggtgagcaac taggaaaaga agtaaaatat acagatgccg gtacgattct tcttgggttg
     1681 atgctagagg aaatttacca agaaactgca gtggaaatct ttcaagaaca agtattggaa
     1741 ccattaggaa tgatgaacag cttatttctt ccttttcctt ccaaaaaaat cgttcccaca
     1801 gaacaactgc cttcgggtga ggttttgcgt ggacaaactc atgatccgaa agcaagagtt
     1861 ttggctgaac atgcaggtaa cgcgggtctc tttattgatt tgaaagacag cattactttt
     1921 gtccaaatgt atttgaacaa gggaaaaatc cgtaatggtc gttttttaga agaagagacg
     1981 attgattctt tactgcaaga tcaaacaccg aaaaaaaatg gctatcgttc tttaggatgg
     2041 gacttaaaaa aggatgtaga agacggtcat gcgattcttt ttcatactgg ttatacaggt
     2101 acgtttttct tgattgatat aaaaacacaa gaagcgttga tatttttgtc aaatcgcgta
     2161 catcctgtcg atcatcgtgc ggagtatctc atatgtcgag atgaaattgt tgaaaacttt
     2221 ttgaaagaga aagcggtatt accgaaataa tgctataata gctatgaaaa atgagaggag
     2281 tttgaattat gcaggaacct atgtttttaa aaccagtttt tcaagaaaaa atttggggag
     2341 gaagtcgttt acgttcagtc tttggttttg atatccctaa cgataaaatc ggagaagatt
     2401 gggcgatcag tgcgcatcct catggtgtca gtgtagtaga aaatggcgaa ttcaaaggga
     2461 aaagattaga tgaactatgg tcagaacaca aggaattgtt tggccatcct tcagaaccag
     2521 tgtttccatt attgatcaag atcttagacg cagaagatga tctatctgta caagtacatc
     2581 cagatgatgc ctatgggatg aaacatgaag gtgaattagg aaaaactgaa tgctggtata
     2641 tcatcgatgc tgaacctggt gcagaaatca tctatggtca tcatgcaaag acaagagaag
     2701 aactagctga gatgatcaaa gatgggcgct gggatgacct tttgaagaaa gtaccagtta
     2761 aaaaaggtga tttcttttat gtaccaagcg gaacgatcca tgctattggt aaaggaatca
     2821 tgattttaga aacacagcaa agttctgaca cgacttaccg cgtgtatgac tacgacagaa
     2881 aagatgcgca aggacaaaca agagagctgc atatccaaca atcgatcgat gtaacaactg
     2941 tccctgcgaa aacaccagaa ttacaaatca aagaagtccg aaaagggaat tcatcgatcg
     3001 tcacttattt agaaactgag tttttcaatg tatatgagtg ggatattaaa ggaatcacat
     3061 ctttcaaaaa acaagcccct tatacattga tgacggtaat cgaaggagca ggagaccttg
     3121 tcgttgacgg aaaaacatat ccattagaaa aaggaacaag ctgtattatt ccaaatggcg
     3181 tttctgagtg ggcggtccaa ggtgaactag caatcattgc gtctgaacca ggcaaatagt
     3241 tcccagatag ctgaaagcag cagacggttt ttcgtttgct gctttttcta cataaaatcc
     3301 gtccatcaat aaatagaagt ctggtgaaaa agatggaata caaatggaaa gcaaacaaca
     3361 gcagtcgcgt gtctatgaaa caactgagaa aagctggaca tcacttaaat gaaatgatca
     3421 tgccaaatct aagtctgttt atcgcttggg gaatcttgtc attagcaaca aaaggtgtgt
     3481 ctggcgattt gcacaccaca ttagaagagg tatgccattg gatgatccag ctgcttttac
     3541 ccatcttgat cagttattca ggcgggaaat cggtaggggg acaaaaaggg gcagtcgccg
     3601 gtgcaattgc ttctttaggg ttgatcgctc gttccaatgc tccacaaatc gtaggagcga
     3661 tgattatcgg gccgcttgcc ggcatcgtat atgaacaatt tgtttctaga ttccatgtga
     3721 aattcaaggc tggatatgaa atgcttgtca gcaacctact agtaggactg actggtagcg
     3781 ttatttgtat aattggaatc gtttttattg aaccagtttt aaatagtgtt cacttgttgc
     3841 tcgcctccgt tgtgtcttgg ctagtcacaa tgaatctgtt accacttgtc agtctgatct
     3901 tagaacctct aaaagtacta tttttcaata atgcaatcaa tcatggtgta ttgacacctt
     3961 taggaatcga attctcacaa acagtcgggt actcttcctt atttttgatg gaggcaaatc
     4021 ctggacctgg tttagggatc ttactttcta tcctttgttt tgcagaaaaa caggaaaaag
     4081 taaatgcgag tggagcattg atgatccatg cgattggcgg gattcacgaa atctattttc
     4141 catttgtctt gctgcgaccg tatctatttt tagctgtaat ggctggtggg gcaagcggca
     4201 cgttgatatt tcaatggtta gatacgggat tagctgcacc tgtatcgcct gggtcactac
     4261 tcgtgatttt tgccaatgcg ccttatccac aactagctgg ggtaagtata gggatagcaa
     4321 tcagtacatt tgtgaccttc atttgtgcag cgatactatt gaaattacca aataaagaaa
     4381 aagagaacaa aacggtcaaa gaaaggggag aaatcttgga accatctatc attcattcca
     4441 tcatttttgc ttgcgactca ggtatgggat caagtgctat gggagccgcc ctgctgaaaa
     4501 agaaattagc agcagaaaaa atcgatcttt ctgtcggata cagctctatc tatgaattga
     4561 ataaccaagt aaatcagctg gttatcgtgc aacatgaact agcgggtctt gcaagagaaa
     4621 aagtacctca aagtcaaatc tatgtactgg atcacttttt ggaaacagat atcttgctgt
     4681 cacgaataaa agaagcgatc caatttccaa agcgggaaga aaaaaaagag atagcagata
     4741 tcacagaaca aaaaacgtcg cctgctaata ttgtattttt gtataaaaac aacagacgcg
     4801 gctcacaaac tatgggcatg acgattttaa gtcaattagc aaaaaaagcg aaaaaacagt
     4861 ttgttatctc aaaagaatca atagaaaaaa tcaaaatcca agaagaaacg atttatatcg
     4921 cacaaagaga actagctgta actgaacagc tagaagaaaa gatccctcgg ctagttctag
     4981 ttgaacattg ggtagctacg aatgaatacg aaaaactcgt gaagggagaa atataagatg
     5041 tatttatcgc ccagagaaaa acaattatta gcagaatttc tcagtagccc gtcacctgta
     5101 tctatccaaa aaatgatgaa tttattgaag gtcagtaaac ggactattta ccgcgaatta
     5161 gataatcttg aagtctcatt gaaatcggta aatgcttctt tgaaaaaagc agctagaggc
     5221 tcatttgtca ttgaggcatc agaagaagta ttagagcaat taaaagaaaa tacagatggg
     5281 aaaagtaatg acttatccac tgctaaacga caacacgcga ttttagccga gttgttactg
     5341 acgacagagc cgttgagtct tgctcatttc atggaatcat atcgcatcag taataccact
     5401 ttttatgcag atatcaaaca gttggaagaa agtattcgcc aactgccttt ggaaatcatt
     5461 cggaaccagg gatacgagat cgaaggaccg gaaaaatacc gccggcttct gacagcgaat
     5521 gtattagaat tagaaatcaa tgaatatgaa ctttttcata gtatttcttt cgatgcatca
     5581 ctgaattatt tttttcgatg cgttgatccg caacatctat ctcttgcacg caaagtaatc
     5641 ggggaagaat tgatccaaaa aaagaccaat ctaagtgata gaaagcttga acatttggtt
     5701 ttgatgctga cattgacgat ggagcgtgtg acgaaaaatc atttactgac aaatgagaca
     5761 tataatagtc tggcaaacaa agaattacta cagacagcta aacgattgtt ttcacggatc
     5821 gcttctgaga caaaacaact ttatccagta aatgagatcg tcttttttgc aggattatta
     5881 aatgacttca cgaactcatt tgatcagaat ttctttgaag agacgttcga tacacagctt
     5941 gcttatttag taaaacaact gattcaagaa gtaagtgaag agacgaatgt ccatttttat
     6001 gaagatgaaa ctttgtacaa aatgctgttg actcatttat ctggtgtgtt ttcaagagcc
     6061 gttttacaag aagagacact gaccaatccg attttagaaa aaatcatggt tcaatacgag
     6121 gaagttgcga atgcaatccg taaagcagta gagcggattt ttcaagaaaa aaaattatcc
     6181 gaagaagaga tcgcttatat ggtccttcat tttgcgaatt cattggaacg aagtccgaaa
     6241 gaaagcaata tcagtattgc aggattttcg ccaagtggat tagcttctac tagcatgttg
     6301 gaactacgat tgaaaaggta ttttcccttc atccaacaaa ttcatttcta tcggattgct
     6361 gatttaaaaa aaatcgattt acaagaagaa tatgatgttg ttatttctac atctatttta
     6421 ccaggatacg gtggaaaata tattttagtc tccccgctac tgctagaaga tgaagtcaag
     6481 cagctaaaag aagctttttc gcatattgag aagaaaaaaa ggcaaaaaat tacgtataac
     6541 agcaataacg agtggatgat agaagatact tatgaggaaa caatgcattt catggatcaa
     6601 atcaatcaat tactcggtcg tttttttatc caagaactgg ataatcctga gacgatttcc
     6661 gaaacgattc gcagcttatt tccttatttt cctgaaggac tagtaacgga tgaagaggtg
     6721 gttcatcgcc ggctgcttaa gcgatatgaa caagcaccta tcggtattcc tcatacaaat
     6781 atggggttat tccatacatc cagctcattg atttctaaac cgatcttttg tattttcaat
     6841 ttgaaacatc cgttgaccat tgaagggatg gatagacagc cagtcgattt gtcccgtatg
     6901 ttagttatgc tggcgccttc gccaatccaa gaaaaagacg ggattctttt aggaaagatc
     6961 agtggagcaa tcatcatgaa tgacttgaac accgagatct tccgctcagg aaataaagaa
     7021 atcgtttatc agttgttagc gaaaatcctg attgaagaaa taaagaaatg aaggtgaaaa
     7081 gatgatacag ctaccagcag aaaaaatatt gctcgatcaa tcctatcaaa attggaaaga
     7141 agcgttgacc gagatcggcc agtggatgac ccaacaaggg ggaatcaaac cggcatatat
     7201 ccaatcaatg atcgaaagac aagaaaaagc gagtgtctat atcgggaatt tcgtagcctt
     7261 gccacatggg actgacaaag atgaagaagt agtagaagaa ggaatctatt tagtccaagt
     7321 gcctgatggt gtggactttg gaacaaaaga ggaaccaaaa atcgcgacac tcttatttgt
     7381 agtggcttta aaaaaagaac atcagttagc ggtgctccaa gatttagcct ttttctgttc
     7441 agacatcgac caagtaatgg cgctcagtga tgcggaaacc attgaagaag tccaaatgat
     7501 ttttgaacat ggacatatat aaccaatgtt cacagtaaaa aaagctgcct tgcatcaagt
     7561 aataacttca accaagataa aaataagagg agctattttt gttgggaggt tcttattact
     7621 gcggatgcaa gcttttgaaa actatttatt ctagaaaacc gtttatttga aataggggac
     7681 ggtgacaagg tttttgtcac cgcccctatt atttttgtgt tttaatagct cttttttata
     7741 tagatagtgc ccatacctat gaataataaa acgatactcc atataccaag tgtcaagata
     7801 ccattcatga aagtaacctg ttctgaagct agtggcgaga aaatatctcc tcctatgatc
     7861 acattttgaa aatcaacata agaagttggc aagtaatgac tgattgattc tatccatcca
     7921 atcgaagaaa acaagcgata ctctgctaag aaaagacaaa gaagtgtacc taaaagattg
     7981 actagagaat gttcaaataa agcagataga agaaaactga ttgtacttaa aaagagcacc
     8041 cacaacaaca ggaacagaaa gctctggaaa agatactcat acatcggcag gattcgtatc
     8101 tcctgaccaa tgatcgttcc tacaggataa cggaagtctc ccaaaccatt tttgattatt
     8161 actagtatag agacgatcaa aaatggcatc acgagactta gcatagctgc accataataa
     8221 atggtaaaac ggatgctatg tttctttacc atactatccg gtaatagatt gactagagag
     8281 attacctgct ttcttttttc ataagagatg atggaagaaa gaaacaaggc ggttatacat
     8341 aaaatcaaaa aactagaaat cattcctgaa aaaatatttt cgtagtaatt tgctaaagag
     8401 agatgcgctg gcaagtcaaa aatagaatat ctttggatct gatgttcttt catataacgg
     8461 agcaactcga cacgtttttg ttgttcaatg attggaattc cttgtctttg accagaaatc
     8521 agtcgatcca gtatatcttt ttcataatga taagttgctt cgaccatttg ctggacattc
     8581 cctgtgtttt ttgcatgtaa gattgcttcg atcagtttgt tgcttttctc taaactttcc
     8641 acaaattctt tctcattctc atcttctttc aacgtttcaa tcaaacgttg catctcttca
     8701 tagttatgat tcagttcatc gaattctgta taagcaagtt tatgatcaaa aaaaagtggg
     8761 aatgcaaaga gcacgaccaa caatagagga aataaaaagt ttactttgtt ttttagtaag
     8821 agattgcgtt caaatgtaag taaagacata gttacacctc atataattta cgataaatag
     8881 ctaaaatctc ttccatattt gaggattctg tgacgatttc tccatttttt agaaaacatg
     8941 ctttagaaca aatcgattgg atattcgcga tagcgtggga agaaagaaga atcgtcttcc
     9001 cctctttctg ccattgtctg atgaggctat tcaagatatc aatacttcct gggtctaatc
     9061 cgttcaacgg ttcatcgaat aacatgacag atgaatcact gatcaagtaa gcagcaagca
     9121 agacatgttg tttcattcct agagacattt ttccaatcgg caaagtaagg tactcagtca
     9181 tcttcaatcg ttggaaaaca gtttggcatt ctactttgct ctgccaatag tatttgatca
     9241 aagtcaaata atcaacagcc gatagatagt cggataactg attcgttgat tctagaaaaa
     9301 agaaagaccg gcgataatct gtataagcag taggagagat gttgtgaagg gacaattgat
     9361 cataagttgt tggcagcagt tgggcaatgc cattcagcaa agttgttttg ccaaatccgt
     9421 tcggggcaat caaagcagct acttctcctg attctactaa aagattcaat tgtcgaatca
     9481 cacgtttttt accacgtttc atgttcatgt ttttgatcag taatgtcata gataggcctc
     9541 caatataaaa tttaataaca taatagaatg gaaaacatct aaattaaatg ctaacaaaag
     9601 ttattgtttg catattataa aaaaattaaa ataacagttt tatttaaaat tgaagttttc
     9661 tgataccgaa atctttttat tttgctgaca tactcaaacc tttggcacaa agtttcgtgc
     9721 caaagtacga ctatttctct ttttagtaag cggatacaat tagagtatca aaaggagaga
     9781 gggagagaca aaatggaacg gacgattccc aaaaaacaac atttcgctaa aagcgagggt
     9841 acaaaaatta ggcagtactt tatcaagcat ggtcatgcca aatattggag cactgattgc
     9901 ctggggtgta ttaacggctc tatttatccc agatggttat ttaccaaatg aggctttcgc
     9961 taccatggta agcccaatgc taacttatct gattccttta ttgatcggtt atactggcgg
    10021 aaaagtaatt gctggtgacc gcggatcagt tgttggggca attgcaacga tgggtgtgat
    10081 tgtcggaaca gatattccaa tgatgctagg cgcgatgatc atgggacctc ttggtggata
    10141 tgccatcaag aaatttgatc agctattcca aaaacggatc aaatccggtt ttgaaatgct
    10201 agtcaataat ttttcagcag gtcttatcgg ttttggatta gcgttattag gtttttctgc
    10261 gattggtcca gttgtcgatg cattgactca agcaatggca aaaggtgtag agatcatttt
    10321 atctgcccat ttgattccat tgacaagtat ttttattgaa ccagcaaaaa tcttattttt
    10381 gaataatgca atcaatcatg ggattttgac accattggga actgaacaag tagcagaggc
    10441 tggaaaatct attctattct tgcttgaagc aaatcctggt ccaggacttg gcgtattact
    10501 tgcttttact ttgtttggca aaggagcagc aaaatcttca gcaccaggag ccatgatcat
    10561 tcacttttta ggcgggatcc atgaaatcta ttttccttac gttatgatga aaccgttatt
    10621 attcttatca gttataggcg gaggaatgtc aggaagtttc attttccaat tgatggatgc
    10681 tggactaaga gcgccagctt ctccagggtc gatcattgcg attctagcga tgacaccgtt
    10741 caattcttac cttcctgtta ttttgggcgt gattgttgcc gcagctgttt cgtttggtat
    10801 ttctgcagta attttgaaag cggatgcgaa agatacaaat gaagacttcg aaaaatctgt
    10861 tcaagagaca aaggcagcta agcaagcttc caaaggtgcc caaggtattc ctcaaggagc
    10921 tacattctct ggcgtacaaa aaatcatttt tgcttgtgat gcaggaatgg gatcaagtgc
    10981 gatgggcgct tcgatcttga gaaaaaaagt acaggaagca gacttgcctc aaacggtgac
    11041 caatcaagca atcagccaac tgacagatga ctcgaataca ttgatcgtta ctcaggtcga
    11101 actacaagaa agagcaaaac aaaaagcacc gagcgcgcgt tttgtctcag tagaaaactt
    11161 tttgaattca cctcgatatg atgaaatcat tcaagaatta agcagcaagc agaaaacatc
    11221 agtttctgaa attgagaatg agccagcaga agagtttctt tcagcaaaca tcaaacaaat
    11281 caaagagatt ctacttgtcc acgatgatcg ttccggatca gcaacaatgg ggatgaccgt
    11341 gctcaaagat atattagaca agaaccattt aactatcccg ttaagaaaag tcaatatcca
    11401 agatttaaaa gatgaacctg attcactgat cgtagccaaa gaaagtttag cggaaaaagt
    11461 acagatggct gcacctcaat ccattcattt atcagtcaac agcatggtca atccgcaaaa
    11521 atacgaatcg attgcacaat atttgaaaca gacagcttaa gggaggaaaa cagcatgcaa
    11581 agtttgccat tcgacatgat cgagctcaat cgatctttta aaaccaaaga agaagcgatc
    11641 cgctattgcg gtcagatgct tgtagaagca gggtgcgttg atgaggaata tatcgatgcc
    11701 atggtagata gagacaaaat gttatctgtt tatatgggga attttatcgc gattcctcat
    11761 ggaacagatg aagcaaaaaa atatgtgaaa aaatccggca tctgtgtcgt tcaagtacca
    11821 gatggtgtag attttggtac cgaagaagaa gaaaagattg cgactgtttt gttcggtatt
    11881 gcaggagtcg gagatgagca tttgcaactt gtacagcaaa tcgcacttta ttgcagtgat
    11941 atggacaatg ttgtccagct agcagacgcg ctcagcaaag aagaagtaac ggaaaatcta
    12001 gcaattgctt aaggagagag aaaaatgaga gcagtacatt ttggtgcagg aaatatcggt
    12061 cgaggattta ttggagaggt tttggcagct aatggatttg agattacttt cgtagatgtg
    12121 aatcacacga tcattgatgc tttaaatgaa agaaaagaat acgaaatcga attagcagat
    12181 gaatcaaaag aaaaaatcac tgtaaaaaat gtcaaaggaa tcaacaatca aacaaatcca
    12241 gaaaaagtaa tccaagaaat cgctgaagca gatttagtta ctaccgccat tggcccgaat
    12301 atcttacctt ttattgctga gttgattgca aaaggtatcc aagaaagaga aaaggaagga
    12361 agcacaacat ctttagatat cattgcctgt gaaaatatga ttggcggttc agcatttcta
    12421 gaaaaagaag tgtataaata tcttccggaa acatcattta ctgacaaata tatcggcttt
    12481 ccaaatgcag cagtcgatcg aatcgtccca ttacaacacc atgaagatcc tttattcgtc
    12541 caagtggaac ctttcaaaga atgggtcatt gacgatagcc aaagaaaaaa taaaaaaatc
    12601 caattaaaag gggttttata tgtagatgat ctagaaccat atatcgaacg caaactattt
    12661 agtgtcaata ctggtcatgc gactgtcgct tatacaggtg ccttgttagg ttaccagacc
    12721 attgatgaag caatgcaaga tgcgttagtt gtggcacaat taaaatcagt tttgcaggaa
    12781 acaggaagtt tgctgattgc caaatggggg tttgatgcag agcagcatca tgcgtatatt
    12841 gaaaaaatca ttcatcgctt ccaaaacaaa aatatctctg atgcgatcac acgtgtcgct
    12901 cggacaccat tacgtaagtt aggttatcaa gaaagattta cacgtccagt gagagaacta
    12961 caagaacata acttgacttg cccacatttg acggcaacaa tggggattat ttttaattac
    13021 tatgatcctg aagatgaaca aagccgtcaa ttgcatgaaa tgaagatcca tgaaaacttg
    13081 gaacaattga ttcaagaagt cacaggaatc aatgatccga aaacaatcgg gaatatcaag
    13141 caaaacgtga accgttatgc caagcaagtc gcttaaaata ggatacgaaa aaggggaatg
    13201 tgacgaaatt tttgtcacat tccccttttt cgaataacta ggctgtttgt ccttcaagat
    13261 agggcagatc caattcttta ccgtaacgtt gttctaaagc taggcggatc aaacgagctg
    13321 ccaggttctc atttcttgca aggataggtc catggaaata agaaccgaag acatttcggt
    13381 aaatgacacc ttcagattgg tcttcaccat tatttccttg accttgtacc actttcccca
    13441 aaggtcgttc accttcacct aagaaagtcc gaccattgtg gttttcaaat ccataataag
    13501 tttcattgaa ttcttcgtta tggataacga tatcaccgat aaaccggtta ttttcctgac
    13561 ttaacgtgta gtgatccagt gcactgattc cttggatctt ttctccatta gctcccatgt
    13621 agtagtgacc tagcaattgg tagccaccac aaatggctaa catgacgcca tcattttcga
    13681 tatagttgat caaagaatct ttctttttct ggatgtcttt tgaaatgatt aattgctcaa
    13741 agtcttgacc accgccaaag aaaaccaagt catatttatc agcatcaaaa ggttcatgta
    13801 tactaacgat ctccgtatgg caagaaaccc ccattttttc tgctatatat ttcaacatca
    13861 aaaggtttcc gttatcacca tatgtgttca tcaaatttcc ataaaggtgg gcgatattga
    13921 gttcataatt agccacggtt cattcctcct ttgataaatc cttgagcagc tagttcttta
    13981 cgaagctgca atacagcagt gtaagtagct aaaatataga catgctcagt tggcaattct
    14041 ttgatttttt cgattacttc tggtaattct tttgtttctg tcaatttatt ttctggaatc
    14101 ccggcaactt ttagacgtaa agtcatatct gtatgacggt ctcctccaga aataacagct
    14161 gggatatcca tatcggcaaa gttttcaaaa ttaccatccc aaatccagct gacatcgatt
    14221 ccgtctgcgt aattggcatt taataaagcc actaaagaaa atggataagg agctagacca
    14281 atcatatcga ttacttgatt gatacctaca ggatttttga caagtactaa tgtacatttt
    14341 ttcccattga tattgatgac ttcttgtcgg ccgaatactt tctcgtcata tcctaaacct
    14401 tgtttgattt tatcaggatc gacattgaag tactctgcca ctgcagtagc tgctaaagca
    14461 ttgtagacat tatacatacc gccaacttcg attccgtatt catgaccatc gatcacgaaa
    14521 tctgctgaca cattgtccat ccgtaccata tcagttaatc gataatccaa ttcaggacgt
    14581 ttgaagtcac aatttggaca gtaatatttc cctaagtttg cataagtgat cattttataa
    14641 tgcaagatgt gatgacaatt tggacataag acaccgtctg tattgtaatg agccatttgt
    14701 tcacgatctt cttcatggtc aaagccataa tattttctag gattgactgt ttcgatagaa
    14761 ttgaagattg gtgaatcacc gttactgata atcgttgcat ttggtgactt tgcggcaccg
    14821 tctacaatca ttttataggt cgtatagatt tctccatacc gatccatttg atcacggaaa
    14881 atatttgtaa ataagaacag ttctggtttc atatattctg tgactttgct caaacttgct
    14941 tcatctattt ctaggacagc aaactttttc tgtccttttt ttggttttgc ctgcaagaaa
    15001 gtcgatacaa ttccctgtac catattcgca ccagtagggt tggttagaac ttcatcgaat
    15061 tgctggcgca aaatattgac tgttaaagcg gtagtcaatg ttttaccgtt tgttccggtg
    15121 acaacgacga cttggtagtc ttttgccaaa ctatctagta tatgtggatc gactttcagc
    15181 gctagtttac ctggtaaact tgatccgcct ttaaaaaaag tttttaatgc ccattgcgac
    15241 gtttttccaa ctgcaatcgc aaaatgactg cgtattccca ttatgtaacc tccaaaaacg
    15301 atttgctgac gaataaattc aactaatcat accatatgtt gaaaaaaagg ttaaattgct
    15361 tttcttattc ttaagaaaag gttacggctg aaagtgcatg aaatcggaca aaatcgcatg
    15421 atttaacaaa aaaacagctt gaaacaaaat gattgtttca agctgctgtg attttattat
    15481 tccctcttac ctatattccc attttaaccg ataataattt tctcgcttgg atattcatag
    15541 cctagatctc gtgtttcttt tggcacgaat aacatcaatg tatatagcat tccgatccgc
    15601 ccaataaaca tcagtgtagc aatcgtgatt ttgcccacaa cagacaagtc tccggtaatc
    15661 cctaaagata aaccagttgt tccaaacgca gaagttactt cgatgatgat cgagatcagc
    15721 gtttgttctt ctgtagcaga taaaaaaacg atacaaaaga aacacatacc aagagaaagc
    15781 ataaagacaa cgactgattt tcgcacatca tcttgatcga ttctccgtcc aaaaatattg
    15841 atgttatctt cacttttgag gaaggaatat agatacaaac cgatgattgc tacagtagtt
    15901 gttcgaatcc cgccgcctac agaactagga ctgcatccga taaacatcag taaagaaaaa
    15961 atgatcagtg ttgtgatttg aaaatcaccc aaatcgtgaa tctgcagacc agcattacga
    16021 gtcgtaatcg aataaaacat cgaattgatc catttgacac tcatattgga atcttggaat
    16081 aaatggtcct tttccaatag ataaatcaag acagttcccg aaacaaacaa gataacaaaa
    16141 gctaacacag ctaatttggt aaacaaggaa aaacgaaacg gaagcttcgc attggaacgt
    16201 ttgtataata gccattcccg gcactccatc aatacaggaa aaccaattcc tccgacaaaa
    16261 atcaaaaaca tgatcaggat caagaaaaaa tagtcatgtg caaagggctt gatggaatcc
    16321 ccagtgacgt caaacccaga gttcgtgaca gccgatatcg cctgataaaa accgtaaaaa
    16381 acggcatcac gccaacgatc aaaatagcct ctgtaataaa aataaataga aaagaatgtc
    16441 ccaaaaagta attgaaacca gattaaaata gcaaaagtga tccgaatcag tcggacgata
    16501 ccagacaaac gcggttgatt catatccgtc atgatcagct gtcgctgttt cagtgtgatc
    16561 cgtcgtttag aaaaaatgac aaaagcagta gaaatcatca tgatccccaa accaccaact
    16621 tgaaataata tttccagcag tataataccg ttatcattga acacagaatt gatatcaaaa
    16681 gtcgatagtc cagtcacact cactgtactg attgccatga acagcatgtc cacaaaagaa
    16741 acatggctat ctggttcgcg aaaaatcggc atataaaata gaacgagtga taagaccgtc
    16801 atcaatatat aataaaaaac gatgatttgg atcgatgaga aatgattggc agcaaacttc
    16861 tgtcctcgcc gctgcagcct tcgaaagaat aaatcaacat gcacagtact atcctcctga
    16921 caatagtcgt ttctaccagt atacaagggg aattaggaat actcaaagaa attatcaaac
    16981 agataacaat acagctttcg attaaaatag aaatggctta taataaggaa aatcctagaa
    17041 aaaagaagga ggaggaataa caaattgatt aatgctcaac aaatcgtaga aaaaatgaaa
    17101 aatcaaaaaa taaattatga tacagttctg aaaaaaatga tcatgcaatg ggagaaagaa
    17161 gaagtaaagc ctaagatatt aatacataca tgctgtgctc cttgcagtac gcacacattg
    17221 gaattcatgt ctcaatatgc agagatcacg ttattttttt caaattcgaa tattcatcct
    17281 aaaagtgagt atttgcgaag attacatgaa caaaaacgat ttgtagacca atttaatgag
    17341 aaaactgacc atcatgttgc gctaatcgtt gatgaatacc gtccaaatga attcaccaaa
    17401 atggttttaa caaaccatct tgaaaacgaa aaagaaggtg gtgctcgctg cagtgcatgc
    17461 ttcaatatgc gattagatct tgttgcgcaa aaagcgcaag aactaggata cgattacttt
    17521 ggaagtgccc tgaccatctc gccaaagaaa aatgcagctt tgatcaatca gatcggaatg
    17581 gatatccaaa agatttacgg gacacaatac ttgccaagtg atttcaagaa aaacaaaggc
    17641 tatgaacgct ctttgcaaat gtgcaaagag tatgatattt atcgccaatg ttattgtggt
    17701 tgcgtgttcg ctgccaagca gcaaggatgc gatctgaaag aaatcaatcg ggaagcaaaa
    17761 gcttacttaa aaacatcaac cgtagttttt gaggaaaatt aaagaaatct ttcggaaaaa
    17821 cgtaagaaaa aaataaagaa tttatggtag aaataaggta gactgtataa tttgtccgta
    17881 gtaaagtaag gaaacaagaa taaatttcaa tgaaggaaga gataagatgc gaaaagtagc
    17941 acctattttg aagccaatcg ctgtattaac aatctcgacg attgtcgcga ctactacagg
    18001 tattgtgaat agttccagct accgtgcacc aagttcaagt tctagtactg tagaagaaac
    18061 aacaccaact acttcagtct cgacatctga accaacaaca acggaaagtt tgaatgaaac
    18121 cactacgaca agtacatggg acgatacaac aactagttcg tcgtatgatg agacatatac
    18181 aactgacagc tcaagcgaaa caagtaatac ggtaccagat ggcactccat cggattcaac
    18241 caatccaagt acaagtccgg atacaagtgc tagctccgaa acgataccaa gcagtacgac
    18301 tgtagatgat aacacgcaaa atgatgatgc agcacgtaca gcacagcaaa accaagattc
    18361 aggaaatacc caaacgtata accagcaagc aggtgattct caatgacatt tttactagac
    18421 cttatcaacg gggtgcataa attccttggc tatttagaca tcagtcctaa atatcttaac
    18481 cgtggatata cgattttaag tgtgattccc acgctttatt tattgagaat tgtttatgga
    18541 ctttggcaaa accaaaacta tcttcaattt ttcttatatg gacttgcatt tctagtgctt
    18601 gtttatttta ctgttttaaa tgtattttat tactttttag ataaaaacac gaaagccgat
    18661 gtcactcagc tttttgtgaa gtatttacct gatgaagcgt tcaacatcca gacagaagaa
    18721 tcgatagata atggaaattc gatcgataaa gtgaatacac aagaggtcat ggtttcttat
    18781 gacgaagatt atcaattgaa attagccgag aatatgcggt atttgattgg aaatggtgag
    18841 atcaaaacaa atgaactcgg tcgcatggat ggatttctgg tcgatagaaa tacattgtat
    18901 ccttattact atgtcaagaa aacaggagag aaagagtatg ttctaaatat tggacgaagc
    18961 tactcggaca tggaaaaagt agggaccatt cgacttgact caacagaaga cacattggaa
    19021 cctgtcggat tgttcatcgt cggcggtgat tttgtcaaag acgggttacg ctatcatgag
    19081 ccttatcgtc tgaaattgat tgcaaaaaag gcacagtcgc aggaagaacc agtggtttat
    19141 agtcgtagcc aaagaagaag acattcataa tgtgatgaaa cgccgtgtat cagtggttgc
    19201 gataaaaacg tttattcgga ataaggggga tgtgacacgt cttttgtcac gttccttttt
    19261 tatataacga gaaagataag acgaacacat cacttattct ttcctttcat ttataaacat
    19321 tgtagaataa agaaagtaca acgaagtatt gtgaaaaaac gtaataaata acgaggaaaa
    19381 tacaagtagg aggtaagctg tgaaagaatt tttgaaaaat tgggggattt tgatccttgt
    19441 attagcggct attttattag ctagagtgta cgtgtttaca ccagttactg taaacgggca
    19501 ttcgatggac cctacattaa gtgatggaca acgattgatt tcatctaaga tctcaaacta
    19561 tgaacgaatg gatattatta ctacaaagga accgggggat gaagaacgga tgattgttaa
    19621 acggatcatc ggtatgcctg gagatacagt caagatggaa aatgaccagt tgaccattaa
    19681 tggcaaaaag tatgatgaac cttatctgga tgagttcaaa aaagaatttt cagaagataa
    19741 gttacaagga gaatatgctt atagctcagg attccaagca caagcagaaa gttcatccac
    19801 ctttacgagt gactttgaat atactgtgcc aaaaggaaag tatcttgttc taggcgataa
    19861 ccgcttgatt tcaaaagaca gccgaatgtt tgggttagtc gacaaagata tgatccaagg
    19921 aaaagttgtt ttccgttatt ggcctctttc agaaattaaa atcatgtgat gaaaaaataa
    19981 aaagaagctg tgacatgttt tttgtcacag cttctttttc cgaaaattcc ttcttatttc
    20041 ttggagttgc tcactttttt tgcaatctca ttttaagctt tttggctgat ggataagaaa
    20101 agcgaagcgt ttttgttcat aaatagcttt tccccacgat ggaaggtatg actagtagca
    20161 gagacaaagc ccgttttttc caatcgatta ttcaattctt cttggctaaa gccattatgg
    20221 actttcggat gactaatttg ttcattcttg tcaaagtcta cgatgatcaa ttgaccgcca
    20281 ggagctaaaa tttcataaag ttttgtaaga atattctccg tatcaggaat gtgtaaaagt
    20341 acaagtgata gaaggatgag attagcttgg acagcaggaa attcgacaga agcatcagcg
    20401 tggatcgttc ggacattttt cagatctgct gcttggatct tttcttctgc cattttcagc
    20461 atagtttctg aagcatcggc aatgatcagt tctttgaatc tttccgcaag cggtaaacta
    20521 acaagcccag ttccgcctcc gtaatccagc aggacttttt ggtggctgtc gcgcggtaga
    20581 taagtagtta gttcttcgcg aataatcttg gctaatgctt gacggtcagg ggaatcataa
    20641 tggttagcga tggaatcaaa aatatttttt gtcatcaaat agctccttac gtaaaacttt
    20701 gaaattttaa cttattatag cacgtgaaaa gaaagaaagc atgctatact caattcaaaa
    20761 agggagaatc tagaaagaat atgccgatat cgtttgtaaa agatagagag gaaaaaggaa
    20821 agtgtgtccg agaaatatta ctagacttgc ctgagtggtt tgggcttcct gaatcaacgg
    20881 aaaaatatat cgaggaatca agtaaactac ctttatggtg tgaaaaaaga aaagaagaat
    20941 atttaggctt tatcactctt tcacagacaa gtgaagatac tgccgaaatc tattctattg
    21001 tatgggaata aaaaaagact atcatcacca aggattagga acaaaacttt ttaaagaagc
    21061 agaaggttac gcagcaaaac attacaaata tttgcaagtg aagactgtag acgaggggca
    21121 ctactctata tatgatcaaa ctatttgttt ctacgaatca ttaggatttt caaggttgga
    21181 agtttttcca aatttatggg atgaatggaa cccatgttta gttcttgtca aaaaattgga
    21241 acaaaagtga gcaatagatt taatactaat ctgtgcttga tgaaaattct cactgtaaaa
    21301 aatgctgaaa atcaagtata ataccatagg aacaaactgg aaggtgaaaa agagcatggc
    21361 acaactattt tttaagtatg gcgcaatgaa tagcggcaag acgatcgaga tcctaaaagt
    21421 cgcacataat tatgaagaac aaaataagcc agttgtctta atgacaagcg gtattgatac
    21481 aagagatggt ttaggcgttg tttcaagccg aatcggactg aaacgtaatg cgatgccgat
    21541 atttgaagaa acagacgtat ttgacgtaat tcagaaaatg gaaagcaaac cctattgtgt
    21601 gctgatcgat gaagcgcaat tcttaaaaaa gaaacatgtc attgctttta cgagaattgt
    21661 ggacgaattg gatattccgg ttatggcatt tggtttgaaa aatgattttc gaaacgagtt
    21721 atttgaagga tcaaaatatt tattgcttta tgcagacaaa atcgaagaaa tgaaaacaat
    21781 ctgttggttc tgccacaaaa aagcaatcat gaatttacat tatattgatg gaaaccctgt
    21841 ttatgaaggc gatcaagtgc aaattggcgg aaatgaagct tattaccccg tttgtcgaaa
    21901 acattacttt cacccatcaa tgattacaga aggagaataa gcatgtacga tcaattacaa
    21961 gcaattgaag accgttatga agagcttgga gaacttttaa gtgacccaga agtgatttct
    22021 gatacaaagc gttttatgca gctttcaaaa gaagaagcaa acacgcgcga aacagtcgaa
    22081 gtttatcgtc gttataaaga agtagtacaa ggaatcaccg atacagaaga actgcttggt
    22141 gaaaaactgg atgatgaaat ggctgaacta gcaaaagaag aattatctga attaaaaaaa
    22201 gaaaaagaag tcttagaaga aaaaatcaag atcttgcttt taccaaaaga tccaaatgac
    22261 gataaaaata tcatcatgga aatccgtgga gcagcaggcg gagacgaagc agcattattc
    22321 gctggcgatc ttttcaatat gtaccaaaag tatgctgaag cacaaggctg gaaaacagaa
    22381 gtgatggaag ctaacgttac cggaatcggt ggatataaag aagtgatcat gatgatcacc
    22441 ggagacaacg tatattcgaa attaaaatat gaaagtggtg cgcaccgcgt ccagcgtgtt
    22501 ccttcaactg aatcgcaagg gcgtatccat acttccaccg caacagttgt cgtgatgcca
    22561 gaagctgaag aagtagagat cgatattgaa gacaaagata tccgtacaga tatctatcat
    22621 gcttcaggtg ctggcggaca gcacgtcaat aaaacagcat ctgctgtacg tttgactcac
    22681 ttaccaacag ggatcgttgt tgctatgcaa gatgaacgtt ctcaaatcaa gaaccgtgaa
    22741 aaagcaatga aaatcttgcg tgctcgtgtg tacgataaga tccagcaaga agcacaaagc
    22801 gagtatgatg ccaatcgaaa atcagcagtc ggaactgggg atcgttcaga acggatccgg
    22861 acgtataatt tcccgcaaaa tcgtgtgact gatcatcgta tcggattaac tatccaaaaa
    22921 ttagatcaga ttttagcagg gaaattagat gagatcgttg atgcattgat catgtatgat
    22981 caaacatcaa aactggaagc tatgcaaaat gggtaaaatg acctatcgag aagtcctgtc
    23041 acgggcttct tcttttttag aggatcatgg gaaagaagca tatagtatcc aatttttatt
    23101 tttggaacga aaacaatgga aaaaacttga ttggctcctt catatgaacg aagagatttc
    23161 agaagaagaa cagcggttga tcgaaaccga tctgcgactt ctgcttgcag atcatccacc
    23221 tcagtatttg ctaggatatg cagattttta tgatcatcgg ttaaaggtaa ctgaagcaac
    23281 actgatccct cgaccagaaa cagaggagtt agtcgaatgg tgtttagacg agacgcctgg
    23341 tgtaccgtta gaagtgatag atattgggac agggactgga gcaattgcta ttagtttgaa
    23401 agcagcaaga aaaaattggc atatatctgc tgtggatctt tcagaagaag cattagaagt
    23461 agcgaaagaa aatgctcaga aagaaggaac gaaaatcagt ttttatcatg gagatacatt
    23521 aggaccagta atggatcagc aatttgatgt gattatttct aatccgccgt acatcagtcg
    23581 gaatgaatgg gaactgatgg atgaaagtgt ccggtcattc gaacctaaaa tggccctttt
    23641 tgctgagaac gatggattag ctatttatga aaaaatagca gttgaagcac caagcgtact
    23701 aaaatcaaat ggaaagattt ttttagaaat cggatttcgc caaggtgaag cagtaaaaaa
    23761 aatatttcag caagcatttc cagataaaaa agtggcgata aaaaaggatc tttttggcaa
    23821 tgaacgaatg atccaagttt atggttgaag ttgtggatga aaaatttttt atttgttttt
    23881 ttattataaa aaaacactta tttgacaagg atgtaaacga ctttttggtt tatcaacaag
    23941 tttatccact attgacaaat agattatcca caaacagtgg aaaacttgag gataaactca
    24001 gacaaaataa gaatgtatga aaaaggggtt gtggataaac tgtggaaacg aaacgatatg
    24061 atgaaacaga attgcaagag gcagccgaag cactgaaaga aggagaactt gttgcttttc
    24121 ctaccgaaac agtatacgga ttgggcgcca acgctctttt accaaacgct gtcaagaaag
    24181 tgttttctgt aaaagggaga ccgcaagata atccattaat cgtccatgtc gcctcttttg
    24241 aacaagtaaa agagtatgtg gacaactttc atccggcagc agagaagatt gtgaagaact
    24301 tctggcctgg accattaact ttgatattta agatcaaaaa agacactttg ccctcagttg
    24361 tgacgggagg attatccaca gctgcatttc gtataccgga taacaaaaaa acactagaag
    24421 taatcgaact ttccggtgtt ccgcttgtcg gtccaagtgc aaatacgtcg ggaaaaccaa
    24481 gtccaacgac tgcagatcat gtatatcatg atctacaagg aaaaattacc gggattattg
    24541 atgatggagc tacgcgaatt ggtgtagaat ccactgttct tgatttaagt gatccaacag
    24601 ccatgcctat gattttacga ccaggtgcag taacgaaaga acaaatcgaa gcagtcatcg
    24661 aatctcctgt tgctattgat cagcatcttg taaaagaaaa tgaaacacca aaagcccctg
    24721 ggatgaaata caaacactac tcacccgata cacgtgttct gatggtcaga gatggcgatt
    24781 ggtcaactgc agttcaatgg gcaaaaaata agaagatccg tgcaggtgtc atagcaagcc
    24841 ccgaaattgc ggatcaagtg cggacagata ctgcagctgt ttatatgtat aacgacaact
    24901 cagtagaagc ggcggctaaa ggattgtttg caggtctaag aggattagat gaaccaacac
    24961 ttggattaga tttgattttt gtacaagtgt atccagaaac tggattgggg aatgcttaca
    25021 tgaatcgctt gaaaaaagca gcaggacaaa attattttga aaaatgatca attggttatt
    25081 gtcgcattct tttagtatga tacaatgcta tcaagtaaat cttctatcag tgaacgaaaa
    25141 actaagcgta actaatcgta atagaagaga tacgaatatt tggcaatttg tttttgatgg
    25201 gaaaaataaa attgtaggaa aataaaaagg agctgttgtt tgtggtagat tacaaaacgt
    25261 ttgaccctga tctttgggct gcgatcgcca aagaagaaga aagacaagaa cataatttgg
    25321 agctgattgc ctctgaaaac tttgtctccg aagcagtgat ggctgcacaa ggaagtatct
    25381 tgacaaataa atacgcagaa ggttatccag ggcatcgcta ttatggtggg tgtgaatttg
    25441 tcgatatcgt tgaaaattta gcgattgacc gtgcaaaaga attattcgga gcgaaatttg
    25501 cgaacgtcca gcctcactcc ggctctcaag ccaacacggc tgcttatctc gctttggtag
    25561 aaccagggga cacgatctta ggaatggatc tttccgcagg cggacatcta acacatggat
    25621 caccagtcaa cttcagtggc aaaacctatc actttgttgc ttacggtgtt gatccgacaa
    25681 cagaagttat tgattataat gtggtacgta ttttagctag aaaacatcaa ccaaaattga
    25741 tcgtagcagg tgccagtgct tacggacgaa cgatcgactt tgcgaaattc agagaaatcg
    25801 cagatgaagt aggcgcaaaa ctgatggtag atatggcaca tatcgcagga ttagtagcag
    25861 caggacttca tcctaatcca gttccttatg cagatatcac aacgactacc actcataaaa
    25921 cattgcgtgg ccctcgaggc ggcatgattt tgacaaacga tgaagcatta gcaaaaaaaa
    25981 taaatagtgc ggtatttcct ggtattcaag gcggccctct agagcatgtg attgcgggta
    26041 aagcagtagc attcaaagaa gcattagatc ctgcatttaa agaatatagt gaacaaatca
    26101 tcgccaatgc aaaagcaatg gtcaaagttt tcaatcaagc aattggaacc agagtcattt
    26161 caggggcgac agataatcat ctgatgttga tcgatgttcg cgagttaggt ataaatggaa
    26221 aagaagcaga gagcattttg gacagtgtga atatcactgt aaataaaaac tcgattcctt
    26281 ttgaaacact aagtccattc aaaacaagcg gtattcgaat cggcacgcca gcaatcacta
    26341 ctcgcgggtt caaagaagaa gatgctgtaa aagtggctga actagttgtc aaagcattac
    26401 aagcaaaaga tgataacgct caactagacg aagtcaaaac aggtgtacgc gaattaactg
    26461 aaaaatttcc gttgcataaa aaataaaaat ttaaattcca ttagtaaaca gaaaagttca
    26521 atgttgggct tttctgttta ttttttggga aaacggattc tttgacaata tcggtgatgc
    26581 tctctagtat atgaggtgta attttagtct tcacactaaa tctcagggag gaaaagtcat
    26641 gactgatcgt atctcgttag aaaaggaagc agtagcattc agtgatgcaa atgcgcctca
    26701 tccgcgtatc tatgaactag caccggaaga aggaagagtt ttactagaag aagtacaaga
    26761 atcacctgtg aacaaatatt ccgtcgatat tgaagacttg atggtggata ctggcgaatg
    26821 gggaaaactc aatattcgtc tcgtacgacc agaagggaat atggataaat taccagttat
    26881 tttttatatt catggagcag gctgggtttt tggcagtgct caaacccatg ataaattgat
    26941 cagagaactt gcggttcgta cgaattccgt cgttgtcttt ccagaatata gtcgatcacc
    27001 agaagcaaaa tatccaacag caattgaaca aaattatgct gtgttgcaac agctgaataa
    27061 attagccaag tcaaaaaatc tggatctcga gaatctaatc gttgcaggag attcagtagg
    27121 tggtaacatg gctacagtca tgacgatctt aacaaaagaa cgaaagggcc tcccaatatc
    27181 caagcaattg ctgtattatc cagtcacaaa cgctgatttt gatacagatt cctatcatga
    27241 attcggagaa aactattttt tgacaagaga aggaatgatg tggttttggg accaatatac
    27301 gacgaatgaa gaagagcgtg ctgaaatcac agcttcgcct ttgagagcaa atataaatga
    27361 actaaatgat cttccttcag caatgatttt aacgggagaa gcggatgtat taagagatga
    27421 aggtgaggct tatgcgcgca aacttcgtga tgcaggggtt cctgttacac aagtacgctt
    27481 ccaaggaatc atccatgatt ttgtcatggt aaatgctatg gatcagacaa aggcaacgcg
    27541 tgcagcaatg gcattatcta cttcttggat caagaaaaaa gaataagctt tgcttgtccg
    27601 caggaaaact tcttttcaaa aacggatttt cttaggaaaa atggcgagtt tgactagtca
    27661 atcacttgat tttgttttac aattaaaaag aaacgacaga acaaaggaga gcttaaaaaa
    27721 tgggcaaatt tcaagtgatc gatcatccat tgattcaaca caaactaact attattcggg
    27781 acaaaaactg cggaacaaag gtttttcgtg aagtagtcga tgaaatcgct atgttgatgg
    27841 catatgaagt atcacgggat atgccattgg aagacatcgt gatcgaaaca ccgatcactg
    27901 aaacaacaca aaaaacgttg tctggtaaaa aagtagcgat tattccaatc ttgcgcgcag
    27961 gtattggaat ggttgacggt atcttggaat tgattccagc tgctaaagtg ggacatatcg
    28021 gtctttatcg agatgaagaa acattggaac cacatgaata tttcgtcaaa cttccagaag
    28081 acattgatgc acgtcagttg tttgtagtag atccaatgtt ggcaacaggc ggatcagcaa
    28141 tcatggcaat cgatgcattg aaagaacgtg gcgcgtcgaa cattaaattt gtttgtttgg
    28201 tagcagcacc tgagggagta aaagctttgc aagaagcgca tcctgatatt gacatctata
    28261 cagcttctct agatgaaaaa ttgaatgaac atggttatat cgttcctgga ctaggggatg
    28321 cgggtgaccg tttgtttggt acaaaataag taaatacgaa aaagggtgtg acacaacttt
    28381 tgtcacatcc ttttttggtc atttttaacg tttgatgcag aacagctttt tcacagcttt
    28441 cgtttgcata taaaaaaacc atcccaaaaa tgcagagatg gtcaaattca aatctttttt
    28501 agttctttgt agttaacgat tactacaccc ttactttgcg tcatcaagtt cgcattttct
    28561 gcagtttcat caatctcaac aacagcggaa tttttcaatt gctttgaaat cgttccagtt
    28621 tgcgacttgc cgtgaattgt gaacattacg tgagtattga tcttgaattt ttcaccaatt
    28681 tccgatggta cgatagttgt atcaaatttt ccaaaagttg ccattttcat aaaacctgcc
    28741 tttcacatca attataccac gttttatgaa aaatatcatg aaattaaact aattttccat
    28801 cggactcttt acaatcagcc aaatagagta tataatttct tttggataag gccagcttag
    28861 ctcagttggt agagcaacgc actcgtaacg cgtaggtcgt aggttcgatc cctatagctg
    28921 gcattaaaag agaagacaga tatatcaagg atttttaatc cattgataca tctgtctttt
    28981 tgtttaaagg aaataaagta tgctttctta gcaatagagc atgtattatt tctataaaaa
    29041 tttaggagaa taaaacgact agcaatatag ctgtgaaatc aaatatcaca tgaactgccc
    29101 aacttgtaat gatcgagttg cttttaatgg cagcttggtt gaataacaat cttgccaccc
    29161 cttgtatcaa aaggatgtgt gctacagtgt gtaaaatatt tccattatca taagtactgt
    29221 aatggataaa tccaaagatg attgctgaca agaaactagc gacaggaatg ctgaacttac
    29281 tagaaaaaat agcgagaaaa tacactgaga acaattcttc tccaagtatc atgataggca
    29341 aaataataaa taataacggg ctgaaagcct catttattgg attgcctgtt aaaccccatt
    29401 tcaaaatttg ttgcaaaaat accgagactg cggcactgat tactacattg ataaaaaaat
    29461 atttggctat atttttgaca ggagcgacag gtttcgagaa catttttttc gtatcttttt
    29521 taccaaaaac aagtgtaaat gctataaatc cagtgatagt taaagaaaaa atgctaataa
    29581 tggatggcgc aaatagcaac cctaacaaac cgagtacaac gacaatagtg cccaatatcc
    29641 cttttttaag ttctattttt tttcctaaaa tgttattcat gataataact cctactattc
    29701 agtttttgaa gatagagtca gtatacatga agagttaaaa taggcaatgt ttttttatgg
    29761 atatttgtat tgctaaaaat ttatcttata tattcatttt gattatacta cttttatttg
    29821 taaaaatttt ctagcaaaac caagtgcttg caactgatta agcgtacaat tttttagatg
    29881 ttccgagcgt acttcaatca tttcaaagga gttgctactt aaatcgatat tttttaaatc
    29941 agtatcaaaa aagctagcat tttctaaatc attagtagaa aacattattt tagtgaggga
    30001 acagtctaca aaatctgcgc ttgataagac agagtgctca aaggtacttt cttttatgtt
    30061 tgaaccagat aaatttacat agtccatttt acaattctcg aataacgtgt tcccaatata
    30121 actttccatc aatttagtac ctgttaattt acaattgaca aatttcacgc gatgcaacat
    30181 gctttttgtc aatatacagc ctgaaaaatc acagttttcg aatatacagt cggtaatctc
    30241 cattctttga aaatcattgg aagcaaaaaa tgcatacttg aaaatagatt tgtaaataat
    30301 tggatggcag attatttggt tttcgatgac ctctttttgt gaaaggatat tatacagttc
    30361 aaaatcttcg ttttcataga tatgaaaaaa ggatccttta ttcaagcttg cttcattaac
    30421 tttaggaaaa atcatattta attacctcca ttttcataaa gtcatattaa caaaagctaa
    30481 accaacttac attagcagaa aatgagatag gttttcgcct ttttcgataa cgatagtgtc
    30541 gctatctctt gaaacgttct tagaattgcc ctgagagaca caaattaggc gtaattctaa
    30601 taaaaatctt agaataaaaa tttttactga tttaatttag aatgttttta aaataaaaat
    30661 cgttgccata acagtgtatt gaaaggtttt aacaatccag taatgattta aaaaaatcta
    30721 gatgtagcgt atactttgtt tatagaatgt agtgggaaga aatatatttt tgggaggaaa
    30781 tcgtgatgtc taaaagtgaa gaaatagaaa acatggtact aaaattatat gatctcgttt
    30841 taaatccaga gatcaaagaa aaggaacgta tacttttgat agacgcaaag acaggcttag
    30901 aaaaaggtca gtattatcct aaagtcataa ataatttgga acgtagttta cgtccgttag
    30961 caatcagggg agaattgtct aaaccagtat caccgtttta tatggagata tcaacaatag
    31021 gaaaatttga aaaagaatta ggtcgaggaa tggcttcggc accgattact tttgggcatt
    31081 tataaaaacg agttattcat ctcaatgaat taaaaagtat agtcaatctc ataatgcagg
    31141 ctaggttcat ttatttgaac agagtctgta tttttttatt ctaaataaag aaacagatgt
    31201 ttctttatat tgtattttat cctttaatta acacaaaaac agctagtata aactaataat
    31261 gttaattaaa agtgtatttt atgaaactat cgttatttat taatgttgca tttttgtaag
    31321 ttaaaaattg gaaatataga ttgaaaaaat gaaagcaata agtaaaataa aatgaatata
    31381 tgcgaaaaaa taatttacat taggaaaaac cttgtaaaaa cagttttttg gcatatttat
    31441 tttttataaa tatttgtaaa ataactatca attttttagt taaaagattg gaaatgagtg
    31501 ggggaggaat tgaagaaagg atttttgaga gatgatatag taggttttat ttttagttaa
    31561 aataggggag atagaaaaaa tgaaaaagtg tagaaatatt attttatcaa tgttatttat
    31621 tattactaat ataacatcac tgattcctgt tcatgtctat gcggatgcag gcagagatat
    31681 cagcagtaat gtcacttcgt tgaccgtgga cccaacgaat attacagatg gaggaaacat
    31741 aaaagtaaaa ttctcgtttg acgaaaaaaa acaaaatatc caaccaggtg attatctttg
    31801 gatcaattgg ccaagtgaag gaaatattcg cggtgaagga ttccaaaaag aaatcccatt
    31861 aatgattgaa aataaaaatg ttggaacttt aacggtcaga aaagattctg cgcaggttgt
    31921 gtttaatgaa aatattaaaa acctagactc agtagaaggt tgggggcaat ttgagataca
    31981 ggccagaaac gtaaccgata ccggtgaaga aaatacggga ccattcaccg tgacgagcgg
    32041 tgataaaaca gctactgtaa atgtgacaaa gccagcttct ggttcttctt ctagcgtttt
    32101 ttattataaa acaggagaca tgctacctga agatactaag cacattcgat ggtttttgaa
    32161 tattaacaat aacggcactt atgttgaaca accagttaaa atcagtgatg aaatccaaag
    32221 tggacaaaga ttagatccaa gtacgtttga aattaatcaa atacacttag gtgaacagaa
    32281 ggtttataga ggagaagagg gaatccaaca atttttacaa gatttcccca gcgctacatt
    32341 taatttttca gtaactgata attacattga aataactata ccaaaaaact ttgtcaatct
    32401 taggaaaata atggtgagtt ataagacgat catagaaaac ccagaacaaa taaattttga
    32461 gaatcattcg gaagcttggt ttaaagaatt taataaacct gctgttgatg gagaaagttt
    32521 caatcataca gttaaaaata tttcagcttc tggtggagtc aacggtacag ttagaggcga
    32581 attgaagatt ttcaaatata tcaatgacac agagattggt atcccaaatg tgactttcga
    32641 attaagacga gcagatgaac agcctattca aggacagtct agtatcctgt tgacttcgaa
    32701 tgaacaggga gaagctagta tcaaaggcct gcaagtagga gactatgttg taaaagaaaa
    32761 agaagcaccc aattggattg attttgatcc actaagttca aatgaactca aattctccat
    32821 aaacgaaaat gatacagagg gagtcagttt acctatttat aacaagaaaa aggttacgaa
    32881 catcacagca actaaaattt ggaacggtgg aaccactcca agaccgagta tttattttaa
    32941 attattcaga gcaagtacaa acaattggga accagttcct gatgcagaaa caaaacgatt
    33001 agacaatgga ataacttcag tgacttggga agatatccaa cagtatgatg atagtggaaa
    33061 cgaatatacg tttaaagtgc aagaagtaga tcaaaacgga aatgattatg tcccttcagg
    33121 ttatcaaaaa attgaaaacg ggctagtcgt tacaaatgag aataaagaag tcatttcagt
    33181 ctcaggacaa aaaacatggg aagacaatga gaaccaagac ggcaaacgac cgacgacgat
    33241 cacagtaaat ctattagccg acggtcaacc gatccaacac aaagaagtaa gtgaaaaaga
    33301 tgattggcgc tatcgtttca caaatctgcc aaaatacaga gacgggcaag aaatcatcta
    33361 tactgtaacg gaagacaacg tgcccgaata cagtacgacg attgacgggt ataacatcaa
    33421 aaacagttat acaccggaaa aaacaaatat atctatacca ggtagccata taacaccgtg
    33481 gaaaaatttt ccagagaaga cagagagaga gaaaattaga aatctattct ctcaaaaatt
    33541 gcaaatattt gctcatacag gagaatccag cgaacaaaga agcaattata ttagtaagag
    33601 agctgtacct aaacagataa caaataaatc aaaaagcatt cttcctaaaa caagtagtaa
    33661 aaaatctagt tttgctgtaa tcattggttt attgatagta actatctgtg ctgggatatt
    33721 cttacaaaaa tataaaaaag tttagttaga atataatatt ttttgttaaa tatgactgaa
    33781 atcaaaagaa taaaattccg atttataatc gaaaaaagaa gcatttttat atatgcttct
    33841 tttttcgtag aatcaataaa tatggatttg gctaactatg gaagaatact cgtattagtt
    33901 ttcagcgttc atactagttg ttacattgtt tgattggcca aaagaatttc tgatatattt
    33961 tgtgctaaat aaagaaaaag acgcttgaat tgggaaataa gaaaactact ctatcattac
    34021 aaattttcat aaactggcag atgttctaag taaacaagta tcttttcaaa gtcggaaaaa
    34081 tccataatca acgtagtggt attgatattt ggatgaaagc ctacggtatc ttgacgatca
    34141 atagcagcat cgatcacgac ttggattttt ttctctttgt cgtgcatcaa tgctaatgga
    34201 gtcactgtac ctgctggaac gcctaggagg tcaggaagct gttcttcaga aacaaaagat
    34261 aagcgctttt cttttaattg ttcagctagt tgttttaaat cggcgtgctt ttcatcaggc
    34321 aagatgacaa gatagatttg ttttcctttt cttgatttca ataatagatt ttttacttgc
    34381 ggaccaggta gggaaaaagg gacatttttg actgacgtaa tcgcaggatg atcgactttt
    34441 tgaaaagaga tacccagttt ttttagtagt tcataagctt cttgttcggt tgcacgactc
    34501 atcgcgcatc accctccttt ttttattagt gtatcataaa aaagagttga caaatgaaaa
    34561 ataatcatgt aaattacacg taaagttaaa acaaatgaca tcgaaaagaa gagtagccgt
    34621 taggtgtttt tagagagtct ctggttggtg gaaagaggca gcagttaacc gtgaaacaca
    34681 tcttggagtc gattttctga agcgtatagt aggaaaatcc ggggagcccg ttacagctgc
    34741 ggtcgttgct agatgaccag ataaggctgg cttttccgtg aggagtcggt aaaaaaaggt
    34801 ggaaccacgt gattaacgtc ctttgatctt cggatcaaag gactttttgt tttcatctga
    34861 acggcaacga ctgtgtgagg ttttttctgc aaggaaaaaa taaacagagg tggaaccacg
    34921 tataatacgt cctcttggca tttgccaaga ggattttttt attttaaagg aggaaaagaa
    34981 gatggagtat ttgattgaag ttatgccgca attattagta ggagcactca caacattgaa
    35041 attattttta tttaccttga ttggctcatt accattaggg attgttttag cattaggatt
    35101 gtctttgcat tttaagccgc tacgctactt gcttcaaaca tatgtatggg ttatgcgggg
    35161 aacaccgctg ttgttgcaac tgatttttat tttttatggg ttgccgacga ttgggatcgt
    35221 ttttgatcgt ttcgaagcag cactcattgc ctttatctta aactatgcag cttattttgc
    35281 agagatcttt cgtggaggga tacaatcgat tcctcaaggt caatatgaag cagcgcaagt
    35341 tttgcaatta agtccctttc aaacggtgac gaaaatcgtc atcccacaag tgacaaagat
    35401 tgttttacct tctgtaggga atgaagttat caacttggtc aaagattctt ccttgatcta
    35461 tatattaggt ttaggtgacg taatgcgtgc tgggaaaatc gcgatggaac gcgacgcgac
    35521 tttactgcct ttgctaggcg tggcaatcat ctatcttaca ttgactggta ttctaacaat
    35581 cgtaatgaaa caagttgaag gaaaattaaa ttattaccgc taactgatag gaggaagaaa
    35641 tatgattgaa ttgaagaaag tgaacaaagc atttggtgaa aaaaaaataa tcaaagattt
    35701 gaatctaatc attccagatg ggaaaacatt agctatcgta ggtccttccg gtggcggtaa
    35761 aaccacgctt ttacgtatct tggctggctt agaaagttct gacagcggag aattttattt
    35821 agacggtgta ccgttcaacc cattcgagct taaaatgaaa gaacaagttg taggagtcgt
    35881 attccaagac ttccagctat ttccacatct gaatattttt gaaaacatta ccttagcacc
    35941 aaaaatggta ttgaaacaga caaaagaaga atacaccaaa aaagcagaag aattagcggc
    36001 ttcattaggg attagtgatt tattgaatat ctatccttat cagctttctg gcggacaaaa
    36061 acagcgggta gcgattgcca gagcattggc tatgaatcca cgggtattgg cttatgatga
    36121 accaacaagt gctttagatc ctgaattacg ccagcaagtc gagcgtttga tcctatcatt
    36181 gaaagaagat ggggtaaccc aaattattgt cacccatgat ttagtatttg cagaaaatgt
    36241 cggtgatcaa ttactaaaag tttccccaat tcattaagaa aggagaagat agagatgaaa
    36301 aaaaccagtt ttctattcgc tttgatcgca ggtcttcttc tttttgcagg ctgttcgaac
    36361 aaaaaaactt cagcagataa ttgggcgact atcaacgaag agaaaaaaat cgttataggt
    36421 ttagacgata catttgttcc tatgggattt agagataaag acgggaagct cacaggattt
    36481 gatattgatt tagccaaagc agtctttgaa caatatggga ttacggtaga ctttcagccc
    36541 attgactggt caatgaagga atttgaactg aacaatggaa cgatcgattt gatatggaac
    36601 ggctattcca aaacgccggc aagagaaaag aaagtgcaat ttacaaaacc ttatatggaa
    36661 aacgatcaag tgctgatcac acctaaagaa tcaggaatca caaactttgg acaaatgaat
    36721 aataagagat taggtgcgca aaatgggtca tctggttatg atgtatttac gaaacaacca
    36781 gaagtattaa aagagatagt caaaaaccaa gatgctgttt tgtatgatag ttttaatgaa
    36841 gcattgatcg atctgaaaag cagacgggtc gatggtctgc tgatggataa agtatacgca
    36901 gactattatt tgaaacaaag aaacgaacta gaagacttca acataacaaa aggttcttat
    36961 gagagtgagg attttgcagt tggtgcacgc aagtcagata gtacattagt gaaaaaaatc
    37021 aatgaagccc tagatcaact agagaaaaca ggtgaattcc aaaaaatatc cgataaatgg
    37081 tttggcgaag atgttacgcc gagaaattaa caaatagggg actgtgacaa aagacgtgtc
    37141 acagtccctt tttccgaata aacagtgttc tcctgccaac tccttgtgac tcggagctga
    37201 atggcaggct cacaacctct tg
//
LOCUS       DACEUZ010000010        37112 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.010, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000010 DACEUZ010000000
VERSION     DACEUZ010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37112
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.010"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(136..2289)
                     /locus_tag="IXG44_000450"
     CDS             complement(136..2289)
                     /locus_tag="IXG44_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HAQ1657190.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFAPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKALELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYTPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTAAKPLASAKMDLSAKTGTAETVAEGHPDITTVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHMNLTIAKEIMDAYYDMFMAK"
     gene            complement(2583..3431)
                     /locus_tag="IXG44_000451"
     CDS             complement(2583..3431)
                     /locus_tag="IXG44_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ1657191.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(3655..5595)
                     /gene="thrS"
                     /locus_tag="IXG44_000452"
     CDS             complement(3655..5595)
                     /gene="thrS"
                     /locus_tag="IXG44_000452"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HAQ1657192.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(5932..6111)
                     /locus_tag="IXG44_000453"
     CDS             complement(5932..6111)
                     /locus_tag="IXG44_000453"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HAQ1657193.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(6158..7981)
                     /gene="cadA"
                     /locus_tag="IXG44_000454"
     CDS             complement(6158..7981)
                     /gene="cadA"
                     /locus_tag="IXG44_000454"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAQ1657194.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            8259..10538
                     /locus_tag="IXG44_000455"
     CDS             8259..10538
                     /locus_tag="IXG44_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ1657195.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(10631..11278)
                     /locus_tag="IXG44_000456"
     CDS             complement(10631..11278)
                     /locus_tag="IXG44_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1657196.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(11457..12176)
                     /locus_tag="IXG44_000457"
     CDS             complement(11457..12176)
                     /locus_tag="IXG44_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ1657197.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(12307..13458)
                     /locus_tag="IXG44_000458"
     CDS             complement(12307..13458)
                     /locus_tag="IXG44_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HAQ1657198.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(13675..14415)
                     /locus_tag="IXG44_000459"
     CDS             complement(13675..14415)
                     /locus_tag="IXG44_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ1657199.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(14419..14742)
                     /gene="rsfS"
                     /locus_tag="IXG44_000460"
     CDS             complement(14419..14742)
                     /gene="rsfS"
                     /locus_tag="IXG44_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HAQ1657200.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(14799..15392)
                     /locus_tag="IXG44_000461"
     CDS             complement(14799..15392)
                     /locus_tag="IXG44_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ1657201.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(15376..16023)
                     /locus_tag="IXG44_000462"
     CDS             complement(15376..16023)
                     /locus_tag="IXG44_000462"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HAQ1657202.1"
                     /translation="MNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHLV
                     MAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIELA
                     RGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPGY
                     TTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(16036..16347)
                     /gene="yhbY"
                     /locus_tag="IXG44_000463"
     CDS             complement(16036..16347)
                     /gene="yhbY"
                     /locus_tag="IXG44_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HAQ1657203.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(16363..17472)
                     /gene="yqeH"
                     /locus_tag="IXG44_000464"
     CDS             complement(16363..17472)
                     /gene="yqeH"
                     /locus_tag="IXG44_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HAQ1657204.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNEIQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(17469..17996)
                     /locus_tag="IXG44_000465"
     CDS             complement(17469..17996)
                     /locus_tag="IXG44_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HAQ1657205.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(18176..18961)
                     /locus_tag="IXG44_000466"
     CDS             complement(18176..18961)
                     /locus_tag="IXG44_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HAQ1657206.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(18954..19823)
                     /locus_tag="IXG44_000467"
     CDS             complement(18954..19823)
                     /locus_tag="IXG44_000467"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HAQ1657207.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(19824..21203)
                     /locus_tag="IXG44_000468"
     CDS             complement(19824..21203)
                     /locus_tag="IXG44_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HAQ1657208.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(21211..21630)
                     /gene="fabZ"
                     /locus_tag="IXG44_000469"
     CDS             complement(21211..21630)
                     /gene="fabZ"
                     /locus_tag="IXG44_000469"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAQ1657209.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(21655..22131)
                     /gene="accB"
                     /locus_tag="IXG44_000470"
     CDS             complement(21655..22131)
                     /gene="accB"
                     /locus_tag="IXG44_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HAQ1657210.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVISEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(22134..23369)
                     /gene="fabF"
                     /locus_tag="IXG44_000471"
     CDS             complement(22134..23369)
                     /gene="fabF"
                     /locus_tag="IXG44_000471"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HAQ1657211.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(23388..24125)
                     /gene="fabG"
                     /locus_tag="IXG44_000472"
     CDS             complement(23388..24125)
                     /gene="fabG"
                     /locus_tag="IXG44_000472"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HAQ1657212.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(24137..25054)
                     /gene="fabD"
                     /locus_tag="IXG44_000473"
     CDS             complement(24137..25054)
                     /gene="fabD"
                     /locus_tag="IXG44_000473"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAQ1657213.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEATPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(25161..25385)
                     /locus_tag="IXG44_000474"
     CDS             complement(25161..25385)
                     /locus_tag="IXG44_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAQ1657214.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(25438..26400)
                     /locus_tag="IXG44_000475"
     CDS             complement(25438..26400)
                     /locus_tag="IXG44_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HAQ1657215.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(26410..26850)
                     /locus_tag="IXG44_000476"
     CDS             complement(26410..26850)
                     /locus_tag="IXG44_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ1657216.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            27156..28121
                     /locus_tag="IXG44_000477"
     CDS             27156..28121
                     /locus_tag="IXG44_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAQ1657217.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(28178..28408)
                     /locus_tag="IXG44_000478"
     CDS             complement(28178..28408)
                     /locus_tag="IXG44_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HAQ1657218.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(28494..29381)
                     /locus_tag="IXG44_000479"
     CDS             complement(28494..29381)
                     /locus_tag="IXG44_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAQ1657219.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(29470..30693)
                     /locus_tag="IXG44_000480"
     CDS             complement(29470..30693)
                     /locus_tag="IXG44_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ1657220.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(30710..31789)
                     /locus_tag="IXG44_000481"
     CDS             complement(30710..31789)
                     /locus_tag="IXG44_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ1657221.1"
                     /translation="MLYYISEGGRKVKVLLYFESEKMLAKSGIGRALDHQKRALTEVG
                     ISYTLDEKEDYDILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQL
                     SPIVKKYLVGLYQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQ
                     KFREYFKLSPDQKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIR
                     KIVKEDHPENVLFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVR
                     DIPVYNGWLEDSKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEE
                     LKNVYESVLNEKVSSKLKKVNQVKD"
     gene            complement(31920..33647)
                     /gene="ptsP"
                     /locus_tag="IXG44_000482"
     CDS             complement(31920..33647)
                     /gene="ptsP"
                     /locus_tag="IXG44_000482"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAQ1657222.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
     gene            complement(33647..33913)
                     /locus_tag="IXG44_000483"
     CDS             complement(33647..33913)
                     /locus_tag="IXG44_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HAQ1657223.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            34392..36626
                     /locus_tag="IXG44_000484"
     CDS             34392..36626
                     /locus_tag="IXG44_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAQ1657224.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            36774..37094
                     /locus_tag="IXG44_000485"
     CDS             36774..37094
                     /locus_tag="IXG44_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAQ1657225.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
BASE COUNT        10436 a         8032 c         6469 g        12175 t
ORIGIN      
        1 cttttttgcc gagtaaacgg tgttcaaaag ccagtttctc ggtatgaagt caaatttaag
       61 caaaaatgtg agaagctatt ttcacaaaat ttttcttcat actcgaaact aagcggcttt
      121 ttcacgacct cttttttatt ttgccatgaa catatcataa tacgcgtcca ttatttcttt
      181 agcaatcgtc aagttcatgt ggtcttgttc gtctaataag ttcggcaaga caacactgat
      241 ggcaatttcc ggattgtctg ttggtccgta tgccacgata ttactattga ctgtcgtaat
      301 atctggatga ccttccgcaa ccgtttctgc agtccctgtt tttgcggaaa ggtccatttt
      361 cgcactggct aatggtttgg ctgctgtgta aggatcggta ccatggacaa cttggtggaa
      421 tccttcacgg aggatcgcca tattttctgc agaaatattg accgtatttt ctactgttgt
      481 gccaatttct ttcttcactt cacccaagtt tccattttcg tcattgccat agatcgcttt
      541 gactaggtgc ggctgtactc gtttcccgcc attagcgact gttgaagcat attgagctaa
      601 ttggattggt gtatatgtgt caaactgtcc aaatgccaaa tcggtaaagt tacctggagt
      661 ataccattct ttgccattat ccgcattgaa cttcttcata taatcaacag aacgtgagat
      721 cccaggagat tcattcggca aatcgatccc agtcgttgta ccaagaccaa actctttgaa
      781 agagtctctc aattgctgat aagctttagc ttcttcatct aaactaggta gtcccattcc
      841 aggtgtatag tctagtccta acatctttaa cgccactttg atcatatacg tattagaaga
      901 caattccaat gctttgactg catcaagagc aacttgtccg cttcgattaa agacagagct
      961 tttttctgaa gcaccttgca gacgaatcgg ctcatcaatc aaaacttgat ttccgctgat
     1021 cgcgttactt gcccagcctg ctgtcaaagt accagccttg actacagacc ctggagcaaa
     1081 ggcgctagta atcgtgccta aagcattctc ggtgatttct ttggaacctt gttcatgact
     1141 gaaaccagtc attgctagca cttctcctgt ctgaggattc atagccactg cataagcacc
     1201 aggcgaatac tgtgctttgc cgttgttgat caatgtttga taattccttt tcaaaatttc
     1261 ttctactttt gattggaatt ctgcattcat agataacatt aaattagaac ctttttcccc
     1321 agagaagatt tctttttgat tcgaaacatt tccttcatta tcaagggaaa cttcatattg
     1381 ggttttggtt ccttgcagga catcttcata ttgtttttcc aaataacttt gtcctacacg
     1441 gtcattccga gaataacctt ttgctaggta tttctctgct tcatctgctg gaagtccttg
     1501 cttttcagtc gtcacactgc caagaatact tttcagtgaa cttgctgcat tatattctcg
     1561 tgtccagtct gtgccagtcg aaactcctgg aagttcagaa gcgcgttctg ccacaacagc
     1621 taactcttga tcagtaacac cagagttttt gatatagaca gtgtttaatt catatgcact
     1681 attcatccgt ttaaagatgg tagcaatttt taattgctcc tcgtttaaat taatctcatc
     1741 agctgtgact ttattgacca tcgcactata ttgttcgctg ttccctaatt gcttttcttt
     1801 tgccgaaagt ctttttgtgg cttctttcaa atgtttggga tcagccagcc aaaaatcttt
     1861 tttatctcta tctgtcagat tttcatctac cggaacatcg atcagcgagt tcagcttcgt
     1921 tgcgatttca agcaaatctt tagccgtaac ttgtgttcct ctcgtatatg tgatggcctg
     1981 attcgcttta ttcgtaacta aagcttttcc tgtggagtcg tagatcattc cgcgaggagt
     2041 actttcttgg accgtaatcg tagaagtaga cttcactcgc gcctccatct cttcgccgtt
     2101 cacgatctgc aaataaccca atctagcaat caacgcaaca aataacgtaa atataatgaa
     2161 aaaaagaaaa tttaagcgaa aaggaacatg ggaccggcga aacgttttgt tcttaggttc
     2221 ttgtgcacct tttcttattt tttctaacca agaagtattt aatatttttt tcataaaatc
     2281 tttcatcatt tcctatcctt ccgcctattt acattcatgc ttcattgtac ccaaaaactt
     2341 cttaataaaa aagaaaaaat gggcggagca agaaattctt tacctaattt caagataaaa
     2401 tcaacttttt gtttcacagc aatcacttaa agttacatat ccaactattt ttttcattaa
     2461 aaaataaact tatttttgaa aattatgttt tcttaagtcg tgaaacactt tgatggatca
     2521 tttatttgtg gaaaactacg ttttgttatt ctaaaaaact tatataaaaa atagtaaggt
     2581 ttttatttcg aatccataaa atattcttgg attgcttctc gtgttttttt cactttttcg
     2641 ctaagctttt cgtttttagc catatagaaa taaaaaatga tatcgatcgt agcaaattgg
     2701 gccaaaagtg aattagttgc cgcacttcga ttcgtgattt cttttggtct agatgtttgt
     2761 acgagtacat cggctatctt gctaagcggg ttatttccaa attgcgtcaa cgcaatgatt
     2821 tcgatattca tgcttttcgc cagctcggct aatgcgacta catctgcact tctacctgag
     2881 tttgaaacaa gccaaagcac tttctttttc tcattgctga caagttgcgg gagcaagacg
     2941 tggatatctt tttcaaaaat gattggctta ccgacacgag accatttttg cagaatatct
     3001 tctactacta aagaagaggc gccaacccca tataaaaaaa cagtctctgc cgattccaaa
     3061 cactctacga ctttttggat cgtttcttct tctagaatat ctactgtttc ttttatcgct
     3121 acttgtgcat tgttaccaag tttgtttttg atagagtaaa aggactcatt tgcttctata
     3181 tcactaaaac cttcatcgat tccagtctgt agttctgttg aaatcgtgat tttgaagtcc
     3241 gtcagacttt gaaaaccgat ttttttggaa aaacgtacaa ctgtcggcgc actcaacccg
     3301 gaagcttctg ctaattcatt agctgtcatt gtagggacct tctcaatatt accgataatg
     3361 tagttggcca actttttttc tgctgaagaa taatcttcca tcatactttc aatacgacta
     3421 attacgctca taagttcctc caccctttta atgatagtct atgtaagtat accatatcag
     3481 acccttattc acggtcgtat actggataaa aaacaaaaaa acaatccgcc caacagaata
     3541 tcttatccca tcaatcccac agattctatt gagcatctgt gtctttgagg aatacctgtt
     3601 atctattgtt cggattgctt tctatctact acttttttac aggaaattta cctattagtt
     3661 ttctctgctg taatttttga cttctgcttc catagcatca ataaagatat tcaaatcttc
     3721 tacagctgtt tctttacttc cataacgacg tacgttcact gttgcatctt ccaattcctt
     3781 gtctccaaca actaattggt aagggatctt ttgtgtttga gaggcacgta tcttatagcc
     3841 cattttttca ttacgatcat caacttctac acgcaacccc ttcatttgca aacgttcttt
     3901 gatttcataa gcataatcgc catgtgcatc aacagaaaca gggatgattg ttgcttgtat
     3961 aggtgccaac catgttggaa aagcgccttt gtatacttct gttaaataag cgacaaaacg
     4021 ttccattgta gaaacaattc cgcggtggat aactactgga cgatgcgtat tttccccgtc
     4081 ttctccaaca tatgtcaaat caaagcgctc tggcaataag aagtctaatt gtatagttga
     4141 taatgtttct tctgtaccaa gagctgtttt gacttgaaca tctagttttg gtccgtaaaa
     4201 ggctgcctcg ccttctgctt caaagtaatc tagttctaat tcatccatag cggctttcag
     4261 cattgcttgg gctttttccc acattgcgtc atcatcaaaa tatttatctg tattgtttgg
     4321 atcgcgatag ctcaagcgga aacgataatc tgtaatattg aagtcagcat atactgccac
     4381 catcaattca agcgtacgtt taaattcatc tttgatttga tcaggacgaa caaatgtatg
     4441 accatcattc aacgtcattt ctcgtacacg ttgtaaacct gacaaagcac ctgatttttc
     4501 atagcgatgc atcattccta attcagcgat acgaattggt aattcacgat aagaatggat
     4561 cgtgtttttg tagaccatca tgtgatgagg gcagttcatt gggcgaagaa caagcatttc
     4621 gccatctccc atatccattg gcgggaacat gtcttcttga taatgatccc agtgtccaga
     4681 tgttttgtac aactcaacat ctcccattat tggtgtatat acatgttggt aacctagact
     4741 aacttctttg tctacgatat aacgttcgat cgtgcgtcgg attgttgccc cttttggaag
     4801 ccagaatggc aaaccagaac ctacttctgg ggaaaccatg aacaagtcaa gttcttttcc
     4861 tagtttacga tggtctcttt cttttgcttc ttcacggaga cggatatatt ctttcaaagc
     4921 tttcttgtca aaaaaggctg tcccgtatac acgttgcatc atttgattgt ttgagtttcc
     4981 acgccaataa gcacctgcca ctgataataa tttaaatact tgtatacggc cagtggatgg
     5041 aacatgtggt ccacgacaca aatcgatgaa ttcgccttgt tgataagcag taatcacttc
     5101 ttcttctggc aattcactaa tcagttctac tttgtacgga tcacttgcaa aaatttctaa
     5161 tgcttcctgt tttgaaagaa cacggcgttc gatcggcaga ttttctttga cgattttcat
     5221 catttctgct tcgattgccg gcaaatcttc tgcagttact tggttttgtc cattatctgt
     5281 atcgtagtaa aaccctgaat cgatagctgg tccaacgcca aagtggatat ttggatagag
     5341 gcgacgcatg gcttgggcca tcaaatgagc tgctgaatga cggactaagc caagtgcatc
     5401 ttcatggtct ggtgtgatga tctcaatact tgcatcttcc tcgattggac gagtcaaatc
     5461 aacgagtttt ccgttcactt taccagctaa agcttttttt gctaagcttt ttgaaatgct
     5521 ttcagcaatt gccaatgttg ttgttccaga ctcaaattct ttgactgcac catctggaaa
     5581 agtaattttt atcataatag tttcctcctt gaaatgtttt gattgatcag atcatcaagc
     5641 tgaaaaacaa aaaaatccta gcatctcttg aaaagagata ctaggacgaa aagttcgtgg
     5701 ttccacctaa tgttcaaacg caaatgcgtt cctcaagcgt tgtaacggtc cgaccgccct
     5761 tgcttcaaca agggtttcga aagtggtcat cctttggttt tctcctggaa aagtttcagc
     5821 ctagcttttc ctctcttatc gaagactctc aaattcagtt ggctttctca tcaatcaatt
     5881 atgtgtttat ttaaccacta ataaatagaa aaatcaagtg ggaaactaaa cttatttttt
     5941 ccattcccca tttacaccgt atgttccttt tttcatctca ttgatgatga catggatgtg
     6001 ttcttttggt gcctgcgtgt ttttatgaac agcttccgtg atatctttca tcatattttc
     6061 taattgttcc ggactgcgac cttctactaa ttcgacatgt acgaatggca taatgctctc
     6121 tcctctttct gatcgttctt ttttcaactc gattctatca tactatgttt gtcttgggta
     6181 ctgaatcagg aaaaatggct ggatcttttt ttgctctcaa ggcactcaaa atagagaccg
     6241 tatccacgac ttcttgcaaa acagctccga ataatgcggg gattagtccc gtacttgcaa
     6301 ttaacatcaa aaaaatacaa ataaagatgc caatcagtac agactgtttc gctattctca
     6361 tcgtatctct tgaaatcact actgcttttg ctacacgcaa gagatcatct ttcaagatga
     6421 ccgcatctgc actttcacta gcagctgtag aaccatgagc ccccattgca ataccgatat
     6481 ctgcagcagc aagtgctggt gcgtcattca cgccgtctcc taccatcgca acaggtcttt
     6541 gatctttagg gatactttct aaaatggtca gtttatcttg aggcaagcta cgggcatgta
     6601 cagaatctaa ttggactttt tctgcaatcg tttgagcaat ggcagatcca tccccggtga
     6661 tcatcatcaa atttttcaca tcaagttttc ttagctcctt gattgtcttt gctgcttctg
     6721 ggcgaatctg atccgaaaat aggatcgttc ctgcaaaaga tcgatcaatt gttacataga
     6781 ttgctgtatt ggacgtttct atttcttcag ctgaaacaaa agaggcacgt ccaatcttta
     6841 cttcatgatt attgactact gcttggattc cttgaccggt cacttctttc agttgactaa
     6901 caggatacag ttggctgttt ttcacgttgt tcactaacga acgagctaag atatggctag
     6961 attcctgttc tgcactagct gcatacagaa gtagctcttc tttttttatt ccattgactg
     7021 gctgaatcgc ctctacttcc aatatccctt tggtaagcgt tcctgtttta tcaaaagcaa
     7081 tcgtttttgt acgtgcgagc ttttcaatcg ttgttccagt ttttacaacg atcccgtttt
     7141 tacttgaacg gctcattcct gcaactaaag caatgggcgc agctagaatc agtggacaag
     7201 gtgatgccac taccagtacc tcagcaaatc gtaaagggtc ttttgagaac caccaagaaa
     7261 taccaccgat caaataagca atcaacgtga aagggactgc ataacgatca gctaaacgga
     7321 caaatcgggc aggcttttct tttgattctt cgactagttt aataatcatt tgatactggc
     7381 tgtctgcggc acgtttttca gcgatcattt ggatcgatcc gtctccattt aaagaccctg
     7441 acataatctg atctccaggt ttcttggttt ccgggcggga ctcacctgtc aaagaagatt
     7501 catcgaccga agattctcct ttggtcactc gaccatctgc gggtacaagt tcgccaggct
     7561 tgactagtaa ctggtcatca atatttattt catcgattac tatatcctgc aattttcctt
     7621 ctttgaatcg atgggcaatt cttggggtgt tatctaacaa agactgcagt tctttacccg
     7681 ctcgtttatt cgcgtaatcc tctagactat ctccgcccgt aagcatgatc aatatcatca
     7741 aactagccca gtattctttg acggctaatg ttgcaaggat tgcggtaatc gctaatacat
     7801 ctacgccata tcttcctgaa cgtaacgtct tgatcatttc caaaaacatc aagaaggcga
     7861 gaatccctcc agtaaatgcg acaatcgcaa atgcaagggc cggctggtga aaaacaaatt
     7921 cacttaataa agctatccca ccaataagca aagtaagtaa aaattttttg aaatgtgtca
     7981 ttgttttttc tcctctttta tgtgacttag agaggttagt aataaatttc cagctgcctc
     8041 tttcatctta agcatagtcc ttgactagca ctttttaaaa caataggcct taattgaaaa
     8101 caactatcac tatcagcgtt tccaaaattc atcagttcta caaacaaaaa tattcagcaa
     8161 ggattacttt ttcgattcat cagttaaaaa gtaccttttt tcgaaaaact atgctatact
     8221 tttaattcgt tatttacggc ggaaggagca aacatgcgat gaatgagaaa caactagaac
     8281 aaacgcacgt ggatgaaacg atcgaactaa tccagatcga acaaaatatg ctggatacaa
     8341 aacaacaaga attgactcag gaaatgaatc attcgacaaa agacacagct aatcacaaga
     8401 ttcgagctgg ttccaatgaa gctttttatg aatctgctgt tgaatatcgc caacatgagc
     8461 aggaattgct tttgaaatac catactcttg aagcacagca aaaacgcctg caaacgttgt
     8521 ctgtcatgaa agatagtcct tattttgccc gtatcgactt taaagaagac cgtgaaacag
     8581 aaacattata tctaggtatc gcttctttaa gagatctggc agaagaaaca atcgttattg
     8641 actggcgcgc accaattgcc aatctttatt atgaaggtga gattggtccc gcattttatg
     8701 aaactgaaac agatagaata gatgtcgatc tgcagttgaa acgacaattt aaaatccaag
     8761 aaggacgtat tttatctatg gtagatacgt cggaagtaat caatgatgac tttttgctag
     8821 agatattgga tgaatcatca agcaaccata tgaaaaacat cgtctctacg atacaaaaag
     8881 cacaaaatgc aatcattcgg gacacaacaa gtaaagtaat gctgattgaa ggaatcgccg
     8941 gaagtggaaa gacatccgct ttgcttcaac gtattgcttt tctgttgtat cataatcgca
     9001 agtggctcga ggctgaaaac gttcttctat tttcacccaa ccatttgttt tctgattaca
     9061 tctcgactgt attgccttca ttaggtgaaa gcggcgtgcc tacacagaca ttcaaaaatt
     9121 atttggtgca gctgctgcca gagttttctt tactagatga acagcaacaa gaaagtggtt
     9181 ttttgctagg ggaaaaagat ccgatacaag tgatgaaaag cggattgacc ttagtcgatc
     9241 agattgatcg ctatatccaa ggcatcacaa actacggtcc cttgttccgt gatatgaaaa
     9301 taaatggccg tacgatccta tcaaaggaat cgattcgcca atggtataaa gaaacaaatg
     9361 aacaattacc tttacatcat agactttctc tattacaaac gaagctcctg aaaaaattag
     9421 gcggtctgca aaaagacgaa acacgtcagc aatgggtcaa agatctggcg gaagaacagc
     9481 tccaagaact atacgcaaca gatccaaatc ttgaatatac agaacaaaag gagcgttcat
     9541 tacgtaaaaa actggccaac caaatcgtac gtaaaagatt ccgtcgaatc catcgaggga
     9601 tcatgaatta tcaatttgtc aatataacga aacaatatct ccactttcta caaactgtgc
     9661 cactttctct tctaacacga cataaaatca gcgagaccag gtggcaggaa catttgacaa
     9721 ctgttcgtac acacttgcgt caaagagaat tgcgtcaaga ggatgctgta ctattctttt
     9781 tactgatgcg ccgaacatat ccagtgacgg ttacacacaa agctcgctat atctttattg
     9841 atgaaatgca ggatttcgca ccatcacaag tggcattgct gcaagccttg ttcccaaaag
     9901 caaatttgac tttctgtgga gatctaaatc aaaatgtctt tggaaatgaa acgatcgttg
     9961 gatcattgga agaccttttc gtggaccgtg aagtcactcg tttccaatta acgacaagct
    10021 atcgctccac aaaagaaatc actgattttg ctaatcattt tattgtgaaa gaacatcaag
    10081 tagaaacaac agcaagaaaa ggagaccttc ctttgctcat ccaaggaaca acagatgata
    10141 tcaaattgaa ttggttagca gataccattc gaacgactca gggaaaagcc cggtattggc
    10201 gtacagcaat catagctaaa acacaagaag aatgcgaagc tctttatgct caactgccag
    10261 acgagttgaa acaacaagtc cagctgattt taaatgagag tgactttatg aagcgttcca
    10321 ttattttact tccagccttt ttagcaaaag gtctggagtt tgaccgagtt ttcttgtggg
    10381 atgtagatgc agaacatttt tcttctcagc aagacaaatt gatcctttac accatgtgta
    10441 cacgtgcgat gcatgaattg acccttttta cctccaataa tgaaagtcct ttgttaaaca
    10501 atatggatat ttcatcttat gagaaaaaag tactgtaagt aaaaagacaa ctgctatcat
    10561 taatgttagc agttgtcttt ttatttacaa taataattgc agaaaaagta attcaatcat
    10621 ttaaggtcat ttattctata tataaatcca aatctgtttc aggatatttt aatttgacaa
    10681 gcgaaataat cattttcagt tcttccatgt tttctggata ttctgggtca aataccttgc
    10741 tatgacggcc aatcccgcca ttgtcgatca atcggtaaat tcgattccat tcaatgaatt
    10801 ggtcaattga ttggaaattg ctgattggat agccgccaca atccaaaatc tctgatacac
    10861 gaaatgctag gtatgcacta tcgatcactt tatttttttt cgtgtctatc ttctcatttc
    10921 tttcgataag aattttttca ggacgatgtt tcaagctcca ctctaatagt agaaagaaag
    10981 cctccatccg atactcttct tcaaataacc gaaaggctga atcagaacga cgaaaaagca
    11041 gcggaatcag ataattttgt ctataaatca gcattggtgc gcggtccacc atacactcta
    11101 aataacggtt cagataccct gtaccgacat agtttcctaa tcgatagcct tcaacgccga
    11161 atcttgcgta tttttgcgct aattttgaac aatcagttac gttcacagct aactcttcta
    11221 ctttgcggta accgcttaag atacgttcaa aaggttctaa agtgattttt gcttccatat
    11281 tcttccccgt ccatcttcca catacttcat atcatttcac atgatactta tgctctcatc
    11341 ataccacagg ttgaattaga aaagaaaata aatcacacaa aaaagcaaga caatctgtta
    11401 gtgagattgt cttgcttttc gaaaaagact gaatactttt cgttaaaact ggttgcttag
    11461 tcttctaatg ccacattatg gaacacatgc tgtacatctt ctagatcttc cagtgcatca
    11521 atcatttttt caaatgtttc ttgatcttct ggagatagtg ttacctcgtt ttgagggatc
    11581 atttggatct cagcgattga aaaatcagtg attccttttt cttttaatgc ttcttgtata
    11641 ctatggaaat ccgctacttc accgtaaacg atgatttgtc cttcttcttc cattacatcg
    11701 cgaacatcga tgtctttttc cattaggtat tcaaggatct cgtcagcgtc ttctcctgca
    11761 aaagcaaaga ttgctgtatt gtcaaacatg taagaaaccg ctccggaaac acccatgttc
    11821 ccgccatttt tcccaaatgc tgcccgtacg tctgctgctg ttcgattcac attgttcgtc
    11881 aatgtatcaa caatcaccat tgagccgctt ggtccaaatc cttcatagcg caattcagag
    11941 tatgtttcat ctcctgcacc ttttgctttt tcgattgctc ggtcaataat atgttttggt
    12001 acattataag ttttagcgcg atcaatgacg aaacgtagtt tttggtttgc atgcggatca
    12061 ggatcacctg attttgctgc cgcgtagatt tcaatcccaa attttgcata aatccggcta
    12121 ttattagcat ctttagcagt tttctttgct acgatatttg cccatttacg tcccatattt
    12181 tcacttcgct ttctgatttg atagtttcat aacgcaaaaa cgtcctgttc cattatactt
    12241 gtgaatcacg attctgtgaa gctttttttc tttcttcctt cattcttctc atttttagtc
    12301 aattatctat actcggattg ggatttgccc gaaattctgt tccggaatat tgcctcccaa
    12361 ttgatagatt tgatcacttc taactaacag ttccgcttct tgtgcctctt tttttccgat
    12421 tcgggaataa atagaaatct cttttttttc ttttaagaat cttcttccag catcggtaaa
    12481 tccaagtagt cgaagataat ttttattttg ttggacagga atctcttgct ttttgatatt
    12541 cagcaagaca taacaagata atcgttggat ccgtgtccat gtatatcttt ttgtttttac
    12601 ctttgacaca aaatcctcaa aactatcagc agttgcagct gctgttttca tgcgcgattc
    12661 tattccttcg gtcatctgat agatatctgt cagttccagc aaagtagaag ataacaaccg
    12721 ataacgcaaa tacggccaat aagattccca agtctgcagc gaacaagttg aaagatcttc
    12781 ttctgtctga ggcggcacaa gtccagctat tctttttcct tcttctaacg catggcgaat
    12841 ggctgttgca ctggctatcc tctgatctgt gatgattttt tcatgatagc ctgcactttt
    12901 acgtcggatc ggatgtaatt ccatagggtg gacatacttg gcattttcct ttgcataact
    12961 taatcccaag atatgatttg gcgaagaaaa atcaagcgta atatcgggat acagactgcg
    13021 aaaaacctca gtcatttttt gcggataact atacgtttga tcagacagtt cttgaaatgc
    13081 ctcttctatc ctatcttgat tctcttggac aaagcgtccg aaactgctgt aatcaaacga
    13141 agtctcttct tctgttccaa aacacaaact gtcacagtcc agtgtttgca agatttttac
    13201 tgcaccttta gcaaaatagt cagctgactg aaccgcccat tcgacgggaa gctccactac
    13261 taaatccaca ccattttgta aagcttcatt cgcgcgatgc catttatcga tgatagccgg
    13321 ttcgcctctt tgcaaaaaat tcccgctcat tacagcaatg acgacctctg ctcctgttat
    13381 ttttcgtgcc atttccacat gatagcgatg tccattgtga aaaggattgt actctacgat
    13441 aatcccgcag cttttcatgc gcatcctcct tcatagttat cgataaaaaa gaaatagaga
    13501 caagtaaaaa aaggcaaaaa tcggaaaaaa tacccgaact atataaacta ttctcgcaaa
    13561 cacaatactt ctgcgacgaa aagctgcact gcaagagcaa tgcctatatc gttcggatat
    13621 ataatttatg ttgaagattt attcttttct tgttaactcg agcaggcaaa accgctagtc
    13681 tttgtatgct acaaagaacc agcgctttga tttctctgat gctgcttcgt ctgtaaaatc
    13741 agaaaatgct tcgactttcg aaaagccgct gttttccagc attcttaaat aactttccaa
    13801 tggataggta cgctcttgat gtagttcatc ttcacgaata aatcgaccag acccttcttg
    13861 ttcttcaacg aaaaaggtaa gaaaatgttc gatgctgtgt ttctcgcttc ctggataact
    13921 atcccacaaa aaagcaaaat tatccccttg ataatgatag ctatattctg gaaaaacctc
    13981 atctacttga taggtggagt gtacgtcaaa taaaaatcgg ccattttctt ctaacgcttg
    14041 atagacttca tcaaacactt gctgtacctc ttgtctattt ttcatatagc acagcgaatc
    14101 agaaaaacag gtaattgcct gatattgtcc gatttctgat aaatcgagca tattgccttc
    14161 cacaaattgg atcgatgcgt cttcttcata tgctcgctgg ctggcaatca tcaacatctc
    14221 ttctgaaagg tctaaccctg tcactttata tccatctttt gcaaaggcaa ccgctaatgc
    14281 tccagtaccg catgccagtt ccaaaatatt ttttgtgtct tcttgtaaat gacgtttcga
    14341 aaaagctaac cattgctgat aaagtgtttc gtccatcact tcatcgtaaa tgaaagcaaa
    14401 tgtttcataa gccatatgtt aatccaacca ttcactaaga tcgactagcg gagcatctga
    14461 ccatagtttt tccagattat agaaatttcg ttctggcgca tggaaaacat gtacgatcac
    14521 atctccaaga tcgatcaata cccattttcc gccttctttt ccttcaatac gttttacttc
    14581 atatttgttt tcttcttctt tatcaatgat ttcttctgta attgcgttga tttgacgttc
    14641 gctatttgct gaacagatca tgaaataatc tgccaacaag gaaacatttc gaacgtctaa
    14701 agcgataatg tcttctgccc gttttgaatc tgctgcttca atcgcaattt ttaattgttc
    14761 tttactatct atgatgattt cctcctaata tgagcagctt atttttttgc tacccagtaa
    14821 ttatatgttt cgattgtttt tggataaatc tgttgttctt gcgcaagcaa atgggccaat
    14881 gtatgttttg tttcaaatgc tacagcttcg tctagatcag cccatgcaat ggctcgtgca
    14941 tcttgtaccc caggaaaatc tcgacctggt tcaatatagt ctgctacata aatgattttg
    15001 tcaaggagac tcatcttagc agccccagtc gtatgcaccc ggattgcatg aagaatctct
    15061 gcatcggtga ttcctaattc ccgctctaca aacgaagcgc caacaagacc atgccagatt
    15121 gcattattat aattcaataa ctccggatca aaaccgtctc ttacgattac cattttgaac
    15181 tcctcatctg gtcgttcttt tgcataatca tgtgtcaatg cagcaatact agctttttca
    15241 ggagaagcac cgtatttttt ggcaagagcc acggctgttt cttctactcc aagcacatgc
    15301 ttgaagcgtt gttcgctcat tctcatctgt attttttgca tcaggttttc tctagaataa
    15361 gcagtatatt cattactata gtccatattc atacaaccct tcattttgaa tataatcaat
    15421 tactttatct ggaacaaggt agcggataga acagccttct ttgatctttt gacgtatttt
    15481 cgtggaactg atgtcgatct caggtacatc tacccaaatt acaggatatg gagtatccgt
    15541 tgtgtatcct ggtcgacgta tacccacaaa gttgaccata gaagtcagct catcgatttt
    15601 ataccatttt ggcaaatatt ctaccatatc cccaccgata ataaagtaat aatctgtatc
    15661 cggattgttt tgcgtcaatt ccttcatagt atcataggtg taacttttcc caccacgagc
    15721 taattcaatc gtttcaatct gcaaaaaagg attgtcttcc accgctaatt ccaacatgtt
    15781 gagtcgatgt tttgcatcaa tcgtctgttt ctcatcaaca tgaggtggct gataagaagg
    15841 catcaaaaat acgcggtcaa gacctaaatt ccggccagct tgttcagcca tcactaaatg
    15901 tgcaaggtga acgggattga atgttccgcc caagatccct acttgctttt tcttctctaa
    15961 gaattgaggc atttcttcag gaaataattg tgaacgaaca aatgtcttag cttgcgtatt
    16021 catcatggtc actccttata ttgcttttac ttcttttgat agcctttgat acttttcttt
    16081 tgtggaaggt ttgtataata caataacacg tccaatgatt tggacgattt cgcaatctat
    16141 ttctttttct aatacttgag ctgcttcttc tgcaatctca tctgtatttt gaagtaacga
    16201 tactttgatc agttctcttt tttctaaagc ttcttcaatt tgaacgatca ttgctgaatt
    16261 gatcccacct tttccgattt gaaaaatcgg ctgaagatga tgcgctttgc tacgtaaaaa
    16321 acgcttttgt tttcctctta aactcataat tttccttctt ccttaaatca atgctttgcg
    16381 tttcaataca tctacaccct ttggcgccca gcccgcaatt actactggcg ctgttacagt
    16441 gatccagcct agaccggcaa aaacgatgtc tgttttttct ttcacagaaa actcaaaacg
    16501 aacaagctcc ggaaatgcct cgacttcatc aggacgagga ggctgtaata atccacctac
    16561 atgtttttga taaaattcgt ctgctttttc cagtttcgtt cgatgaatat ttacatcatt
    16621 tgacacataa gcaataaatg aagcacgatt gccgctgata aaatcaaatc ttgcaagtcc
    16681 gcccaagaaa agtgtttgcc cttcattcag ctgataaact ttaggtttga tttcttttaa
    16741 cggtgcaatg atccgcaaat cttttttacc taagtagtgc gccatttgat ggcgatggat
    16801 aattcccggt gtatcaatca aaaaatgccc gtcatctaaa ggaatctcga ttttatccaa
    16861 ggtcgttcct ggaaactgcg aagttgtgat tacatcttgt actcccgctg tatttttgat
    16921 gatttgattg ataagcgtcg attttccgac atttgttact ccgacgatat agacatcccg
    16981 tccatcgcgg tattcttcga ttttttctaa tagttccgtc atctcatgag atttcttagc
    17041 actagttaat aatacatcta ctggacgaat tcctgcttcg taagcacgtt ccttcatcca
    17101 ttgtgttaat ttagtttttt tcaatgattt aggtaatata tccactttgt tcccaactaa
    17161 aagtactgga ttgtcaccga caaaacggtg tagtcctggg attagtgaac cattgaagtc
    17221 aaaaatatcc accacattga cgatcaatgc atctttttgc cctaattcat tcagtaagcg
    17281 caaaaagtcg tcatccgtca atgagacatc ttggatctca ttataatggc gtaatctgaa
    17341 acaacgctga caatagactt ccccggaagc taaacctttt tccaaagccg tttgcggcgt
    17401 gtatcctaat tcaccgggtt tgtttgtttg tataacggca ccacagccga tacattgtaa
    17461 ttcttctgtc attcgattcc acctcgccag cccatatcag ggtgttttgc taatagatag
    17521 gccataattc ttttttccca aaaacgatta aattgtgtct tccaactatc tgttgcgata
    17581 atgggtttta caaggatgct acgtatccct gcacgattgg ctccacgtat atcagtcata
    17641 atctgatcgc ctaccattac tatctcatca tctgctaaat ccaattgttt ctttgctatg
    17701 ttgatccctc ttgctaaagg tttcattgct cgtgcaacat acaaaagatc aaatttttca
    17761 atcgctcgcg cgacacgttt tgaattattg tttgaaacaa caacaacagg gattcctgca
    17821 tttttcattt ctaaaatcca agtaagcaac tcttctgttc catctggatt attccatgca
    17881 atcaacgtat tgtctaaatc ggtcaatact gctttgatgc ctagattctt taattgggca
    17941 ggtgtaatct gatagatggc ttctaccatc catgttggtt tgtaatttga aaacatgctg
    18001 tctcctcatc tgcaaaaaat gaaggagcac ataaagtact cctcaacccc gtattatact
    18061 gcatatcagc aaaaaaaact acctctatac tcgttcgtct ttcagaaaaa gcatttcttt
    18121 acaaaatgac caaaaataaa gattgatcac agaaaaattt agaaacaaaa aacaactaat
    18181 attggcggaa cctcttgtat cgtttttcca gtaatttatg ctgatctaat cgaacaagtt
    18241 ccgtcaatct ttcaatcaac gcttttttca taatgtgtgc gactttatat cgtggcaact
    18301 gctcgccgtt gaacgtttca ggaataactc gatcaacgat tcctaactcg cgtagttctg
    18361 gcgcagtaat tttcatcaac tcagctgctt ctgcggcgcg actaccgtct ttccacaaaa
    18421 tcgacgcaaa tccttctgga gaaagcacag cataaatcgt ttcttccagc atccagactt
    18481 catccgctaa acctagagca agggcaccgc cacttccacc ttcaccaatg atgatggcga
    18541 tgatcggtac ctttaaatca gacatttcga gcagatttct ggcaatagcc tctccttcgc
    18601 cgcgctcttc tgctcctaca ccgcaatatg cacctgctgt attgatcaag gtaatcaccg
    18661 gtctattgaa cttttccgct tgtttcatca gtcgtaaagc ttttcgatag ccttcaggat
    18721 ggggagaacc aaaatttctg cggatatttt cctgcagatc ccgtcctttt tgaataccga
    18781 tcactgttac cggtttgcca tctaatcgag cgattccacc aacaatcgct tcgtcatctg
    18841 caaataaacg atcaccatgg cattcctcga aagaatcgaa gatctcttca aaaaaatcca
    18901 agcttgtcca tcgttcttta gctcttgcta actgaacgac atcatgtgct gtcttatcca
    18961 cgatctatcc atcctttctg tgtatgcaat tcaagaagcg tatggatttt ctgtttcaat
    19021 tccatgcggg gaacaatctg atcgacaaaa ccatgagata ataagaattc agccttttgg
    19081 aaatcttctg gaagttcctg cttgatcgtc tgttcgatga cgcgtcttcc agcaaaaccg
    19141 atcaagcttt ggggttctgc tagaataata tcgccttcca ttgcaaaact ggcagttact
    19201 cctccggtcg tcgggtccgt taaaacagtt agataaaaaa gaccttcatt gctatgccgt
    19261 ttgacagcag cagagatttt tgccatctgc attagagaaa agattccttc ttgcatccgt
    19321 gcgccacctg aagctgtgaa caagacgact ggaagacttt cctttgttgc tcgttcgaat
    19381 aatctggtga tcttttctcc gacaatcgtt cccatacttc ccatgataaa attaggatcc
    19441 atcactccaa taatgaaggg aatcccctga atggttgctt taccagtgag gactgcttca
    19501 tgaagtcctg ttttatcttg cattttttta attttttccg tatattcggg aaatgctagc
    19561 ggatctttgg ttacaagatc cgtatcccat tcttcaaaac ttttttcatc aataatcaaa
    19621 gctagacgct gccatgcacc gattcgaaaa gcgtatccgc aatgagggca tactttttct
    19681 tcaccgatgt ctttcgtata aaggatatgt ttgcaatttg gacactttgc ccacatgtta
    19741 tcaggaacag atggcgagtt agcaggtttg ctctctctat taggattgat ccgaatatat
    19801 ttctttttct taaacagagc catctatgcg cctcctatct ccgtttctgg tgtccaattc
    19861 ggtaaaaatt cttcttgtaa aaatgctgtg ctgtaatcac ctgctatgac tgcaggatga
    19921 ctaatcaaat ccatctgaaa ttctgcgttc gtgatgattc cttctgtgat aaattcactt
    19981 aaggctcgct gcatcttcat taatgcttca aaacgcgtat ttccatggac gattattttt
    20041 gcaatcattg aatcataata tggcgggatc gtatatcccg ggtaaactgc actgtctact
    20101 cgcagtccca tcccacctgc tggaagcagt aagttttgga tcttccctgg agaaggagca
    20161 aaatggaaag caggattttc tgcattaatc cggcattcga ttgcgtgtcc ttggaatact
    20221 acttcttctt gacacacatt caaaggctca ccggctgcta tctccacctg tttttttact
    20281 aaatcgattc ctgtgaccat ttctgttact ggatgttcca cttggattcg agtattcatt
    20341 tccatgaaat aaaaatcgcc ttcttcatcc atcagaaact cgatcgttcc ggcattttca
    20401 taattgactg ctttagctgc tcgaacagca gcgtctccta gtgcttctct ttttgtttgt
    20461 gagatcacta cagaaggaga ttcttccagt actttttgat tgttgcgttg taaagaacaa
    20521 tctcgctcgc ctaaatgaat gacatgacca aatcgatcac ctaagatctg aacttctata
    20581 tggcgagcag gataaatgat tttttccaaa tacatatcat catttccaaa tgccgctgct
    20641 gcttcttgct gagctgaaga aaaatgctgt gggagctctt cttttgattg aactttccga
    20701 atgcctttcc cgccaccacc tgcagctgct ttcagcatca ctggataacc aattctgtct
    20761 gctatttcca atgcttcttc tactgtagat aatacaccag tacttcctgg aatgactggg
    20821 acatttgctt cttgcatcaa ttgacgcgca ttgatcttat ttcccattgc atcgatcgtt
    20881 gcacttttag gaccaataaa tgtgatattg cactcttcac acatggaggc aaatcggcta
    20941 ttttcagata aaaaaccaaa tcctggatga attgcttcgg cttttgtcac gattgcagcg
    21001 ctcagtactt cctgaacatt cagatatgaa tcaacggctt ttgccgggcc aatgcagatt
    21061 gcttcatctg ctaactgtgt atgaagtgct tcggcatctg cttctgaata tatcgctacg
    21121 gtttggatcc ctagttcgcg gcatgcacga atgatacgta cagcgatttc accgcggtta
    21181 gctatcaata ctttagaaaa catatttcac ctatccaatc ataaaggtta attccgcttc
    21241 ggctactttt ttaccgtcta ctttagcgat tcctttgcct attccggctg aagctttcac
    21301 tttcaatatc tcaacttcaa ggtataaggt atctcccggt gtaactttct tacggaattt
    21361 tgctttgtcc aatccgccga aataagccgt ctttcccttg aattgttcaa gagaaagtaa
    21421 tgcaactgct cctgcttgag ccattgcttc aacaatcaat acccctggca taacaggttc
    21481 ttctggaaaa tgtccttgaa aaaagggctc attgatcgtt acatttttct tagcaacgat
    21541 ccgttctcct tcaaccattt cttccactcg atcaatcaaa agcattggat aacgatgtgg
    21601 aatgatttct tttatttctt gtatgttcat tgttttccct ctttcctgct ttgcttattt
    21661 ttgctgaatc ctaaagagtg gctgtccata ttcgacaact gactcgtttt ctacaaggat
    21721 ttctgtgatc actccgtcgg cctcactagt tatctcattc atcagtttca tcgcttcaat
    21781 aatgcagata acttctcctt tggcaacttg atcacctact tttttgaaag ctggtttgtc
    21841 gggcgctggt tgcagataaa cgattcccac gattggcgaa acaacctcga ttccatcaaa
    21901 tactgccgat tctttttctt tcattatttc tggagcagtt gttgcttgtt cagatatgac
    21961 attgactggc tccgaaactg gcaacgtttc gtttgtttga accatattca tgcctcgact
    22021 tgttttattt ttattcatat acaattcaaa ttgaccttca cgcaaatcga attccgttag
    22081 cgaggattga tcaaattgac tgactaattc tctgatttca ttgatatcca aaattattcc
    22141 tcccagcgtt tcatgcacag aaccgcatta tgtccgccaa accctaatga attactcatt
    22201 gcataagtaa aacttgcagg tttgctttcg tttttgacaa ctgtcaagtc aatctcagga
    22261 tcttgttttt ctacattgat cgtaggtggg ataaattgat gttctaatgc cagcaaagtt
    22321 gccaaggctt cgatcccgcc ggctgctccc aataaatgtc cagtcatcga tttcgtgctg
    22381 ctgacgtaag tatctctgaa attttcacct aatgcgattt ggattgcttt tgcttctgcc
    22441 tggtcattgg ctggtgtact tgttccgtga gcattgatat atccgacttg ttccggctgt
    22501 acaccagctt cttccatcgc aagcttcatt gctttagatg caccagaacc atctggagta
    22561 ggagctgtca tatggtaagc gtcacagttt gcaccgtatc caacgatttc tccatagatt
    22621 ttggcaccgc gagcttgtgc atgttctaaa gattcaagaa ctagaacgcc tgctccttca
    22681 cccatcacaa aaccattccg attttcgtca aacggaatcg aaccacgatt aggatcagtt
    22741 gattctgaga gtgctgttaa agctgcaaat ccagcaatcc ccatctcaca aatcgttgct
    22801 tctgttcctc cagcaagaat gacatcagaa tagccatgtt tgatgttacg gaatgcttcc
    22861 ccaattgaat tcgttccgct agcacaagct gttacgatag ctgtacaaat accttttgca
    22921 cctacacgca aagcaatatt tccagctgcc atgttggcaa tcgccattgg tacgaacatt
    22981 ggagaaactc gttttgcacc tttttcatgt agacgaataa cttggttttc gattgttgga
    23041 agaccaccga tcccagatcc gatgatgacg ccaaaccggt cttgattcat tttttcttta
    23101 tctaaaccgc tcatctccat tgcttctaaa gcagcgtaga ttccatagat cgaaaacata
    23161 tccattcgtt tgctatcttt tttaataaaa tatttatcgt aagggaaatc tttgacttca
    23221 cccgctaagg taatccctgt ttctgaagca tcaaatttag tgatcggtgc gatgccattc
    23281 gtcccctctt ttaagctatt caaaaagctg tctgcgtcat ttccaatagg tgaagtgact
    23341 ccatagccag taattaccac tcgattcatt tgtttgctct ccttttctta tccgtgcatg
    23401 accaatccgc catcgacatt gatgacttgt ccggtgatat atggattttg tgctaagaaa
    23461 actgctgttt gtgcaatatc ttctacttgt ccgaaacgct gcatcggaat ttgcttttcc
    23521 gcttgttcct tgactttgtc tgctaaaact tccgtcatat cagttgtgat gaatcctggc
    23581 gcaatggcat tacatgtaat gcctctagtt gcagcttctc gtgccacaga ttttgttaat
    23641 cctactacgc cagctttact agctgcataa tttgcttgac caatatttcc aatcaaccca
    23701 gatacactag agagattaat gatggcccct tctcgctgct tcatcatttt tttcaatacg
    23761 tgctgcgtca tattgaaggt tccaatcaaa ttgatatcca gacatttttt gaaatcttct
    23821 gcatccattc gcatcacaag tttgtcattt gtgattccgg cattgtttac taacacatgg
    23881 atcgatccaa gcttttcttc tgcttctttg atcatttgtc ccgctttctc ataatcagag
    23941 atgtcaccgg aaactcctac gcattttaca ccgaatgctt cgatttcttc tatcttttct
    24001 tttggaatct ctccgcgacc attcaaaatg atatttgctc cagcttttgc aaaagctaag
    24061 gccatcgctt tgccgattcc tcttgtacta cctgtgataa atacattttt tccttttaca
    24121 tccataacgt tcctccttat agtattgcta ttgtttcatc aaatgtctgc ttattctcaa
    24181 cacgcagaac tggtattgtt ttatcgattt ttttcataaa tccgcttaat acttttccgg
    24241 ggccgacttc gatgacttgt tcgatgccga ttgtttttaa cttatggata ctctcataga
    24301 aacgaacagg ttgcatgact tgctgttcaa gtagtcccgc gattgtttct ttcttcatta
    24361 tttccgttgt tgtgttgctt atgataggaa aagaaggttc agagaattga atctgactta
    24421 ggtccttcgc cagttttttt gctgccggtt ctaaaatagc tgtatgaaaa ggcccgctga
    24481 catttagagg gatcattctt ttgaaccctt tttcttttaa tagtaaaaca gcttcgtcta
    24541 ctgctttttc ttcgccacca atcacgatct gctgaggtgt attataattt gcaggagata
    24601 cgatgccgta cttgctagct tcatgacagc tttcctcgat cgtttcaatc ggcgcattca
    24661 tcactgcaac cattttcccg cttccagctg gggtagcttc tgtcatataa gcgcctcttt
    24721 ttgctactaa cgcaactgct tcagtgaagg atagcgcatt actagctact aatgccgaat
    24781 attctcctaa actcagtcct aaagctgcat ctgggatgat tccatgctcc ttcaataagc
    24841 gataatacgc gatgctgact gttagaatag ctggctgtgt atattgcgtc tgatccaaac
    24901 gttcattttc tgtaaagcaa agttcagcca tatcgtagcc gagaatctca cttgcttcat
    24961 caaatgtctg ctttacaacg gcttcttcat ataaatcttt tcccatacct tgatattgtg
    25021 caccttggcc actaaataag aatgctgttt tcatgttttt ctccaatata tccagaatat
    25081 atgtttgatt cttcctattc aaaaaggccg agacattcct atttcaggaa atgtcccagc
    25141 ccccaaccta attttaatga ttatgcagtt tgtttttcta cgtaagtcac taaatcttga
    25201 accgttttga ttccgtcttc tgtttcgatt ttcacgtcaa attcatcttc aatttcattg
    25261 atgatttgga ataaatccaa gctgtcagct tctaagtctt cttggatgtc agtagtcaat
    25321 ttgatttctt cagtttcttt tcctaattct tctgcgatga tttcttgaat tttttcgaat
    25381 accataataa tatattcctc cgatattgtt tgctttttat ttttataatt taacagtcta
    25441 caactccatg agaatacttc cccatgttaa acctccgcca tagcctgtaa atattacttt
    25501 ctgctgtttt cctaaggtga tctttccact agaaactgct tcatccaata agatagggat
    25561 gcttgcagca gatgtattcc catatttatc catattggtc aggaactttt cttgcggcat
    25621 ctttactttt cgggcaattt tctcaataat tctgaggttt gcctgatgca aaagcagata
    25681 atcaactggt ttgtctttca tgacttcttg gatattgttt gcaacgtctc gtacggcaaa
    25741 atcaaaaata tctctgccag tcatttgcaa ataatacgaa ccttctgaat cttctgaata
    25801 gaacggattt ttattttgct ggtagccagc ggtcaatgag ctacttctgg taccgtcagc
    25861 tgctaatttt tctttcagca attttttctg tggattcgct tctaagagaa tcccacctgc
    25921 tccatcgcca aacaacacag cagtcgtacg atcagaccaa tcgattagct tagatagtgt
    25981 ttcaccacca atcaccagtc ccttagttga gccgcagcgg atcatttttt cagccatgct
    26041 taacgcataa acaaatcctg agcacgcagc actgatatca aaagcgatag catgagttgc
    26101 tcccaatttc ccttgtactt gtgctgctac agatggtgtc gtgtaatcgg gcgacattgt
    26161 tgctactaaa ataaaatcca aatctttagg gtttagattt ttctgtttta tcaatttttc
    26221 cgccactaaa atgcataaat cagaagtctc ctgatttgtt acacatcttc gttcacgaat
    26281 acccgtcctt gacgaaatcc attcatcaga ggtatccatc atcttggaaa gatcattatt
    26341 agtaataatc ttttcaggaa ggacgctggc tgttgcagta atcattccaa attgttccat
    26401 taaatatcct cacttatagt cttgcaagaa gtcgtgcaag tttttcagtg catccagcaa
    26461 ggcatgctgt tcctccgtag ccattccgtc taagatgttc ttgaccattt cacgatgaaa
    26521 atgctgatgg acccgaaata gtaactttcc ttttttcgtt agtcccaact ttacaacgcg
    26581 gcggtcatct tcactgcgga tacgttcgac atagcctttt tttactaact tgttaatagc
    26641 agtggtcaag gtcccaacag taattgatag ttctttggct acttctgaag ttgttttttt
    26701 cttgtacatc ccgattgcct cgatcgtgtg catctctgtg attgacagat ctttgaattg
    26761 agattttttc agctcagact cttcgattgt caaaatatca ttgaagacac taacaagata
    26821 atcatttact gtttttaaat ctggttccat gttccaccgt ccactaatta gtttgattat
    26881 caaatctttt gatgttcaaa ctatatctta acttttttca aaatgcaacc ttaaaagatc
    26941 atttttttac tatatgagct ttttttaaga ttttactcat ttaaaacaac tgataaatca
    27001 gcatttataa tttcttttat tagaaaaatc atctatctca catattttga ttatcaaagt
    27061 attttctgtg aacagaacta tagaattccc taaaacttgt ccattatttc aaaatttgtt
    27121 atagtctttt tattcaaagc gtaaaaggag atcctatgtt aaaagaaaaa atgaaaactc
    27181 tccccatgct gccgggtgtc tatcttatga aagaccgaga tggtaaggtc atctatgttg
    27241 gtaaagcaaa acggctcaaa aatagagtaa gcagctattt tcatcagaat aaacagcatt
    27301 caaaaaaagt attacgtatg attcatcata taacagattt tgatttcgtc gtagtagaca
    27361 cggaattaga tgctttattg ctcgagtgtc aattgatcca acattaccgt cctttttata
    27421 accgacaaat gaattatttc agtaattata attatgtaca tattaccaat aagggatttg
    27481 tattaacaga tactcctacg gctcgaacat acgggccttt tcgtctctat aaaaaaatgc
    27541 cgtctatttt gcgtataatg gaagaaactt atcaaatgcc ttggttgtct gaaattagtt
    27601 tgttagcttt gcgagtccag cttcctgacc tacaggaaat gacttttgaa cagaaaaaaa
    27661 aggaactcca agggctgttt caaggccgaa ataaaaagct actgacttac ttgaaaaaaa
    27721 gacaacaaca tttcatctat cagttgaatt tcgaaaaagc cggtatgctt caaaaagata
    27781 tcgaattagt tacttatttt attagacgga tccaggaaca aaagcagttt ttaagaacac
    27841 catcgctcac cttcagcatg ccattagcag cagatgagag tcagaaaaag cactacctcg
    27901 tttgttatgg tcaacttgca gaaacaatga ttacctctgg tgacgtttct cctgacttct
    27961 attatgaaaa gaaagaagct catttatctt tgaaacgtca attgtcaaaa gaagaaatcg
    28021 atcctgtaca aatcttgatc agttaccgca aaaaattaga aaaagaacag tttgaaattg
    28081 aactgttaaa caaaaaagaa gctgaaaagc aactaaatta gttgcttctc ggcttctttt
    28141 aggcatcttc aacttcaaat gtcaattatc ctaaaattta ttggattaag tcatagattt
    28201 ccatcgctac gatatcgata ttatcaaatt cgtattgctg atgagtatct gtttcagtta
    28261 attcaaatgt atcggtcgct ttatcataag ttactgtaca cttctctacg ccttcttttt
    28321 caaaacggcg aacttgcagt tcattatctg ctgactcgac catagcctcc agtcgtttta
    28381 tgattgcgac taattgtgaa tgtgtcatcg gaggtcctcc ttttcttatt tctgttatat
    28441 tgtatcaaaa gcatagccta cttgcattga ttttctctaa aaaaactcgt tttttatgat
    28501 tttttttctg gtatttttcc ttcaggccac caaagtttga tcaaagcttg ggttccgtca
    28561 ttctcaaaac ctttatctgc caactcttca tatagtttca atgcttgggt agttgctggc
    28621 aatacaagat ccattttttc tgcttcttct aatgcaatct ttaaatcttt gataaaatgt
    28681 ttgacaaaaa aaccaggtgt gtaatcttct tttaaaatac gaggagaata attgcttaaa
    28741 gaccagttag ctgcacttcc acctgaaagc gtctcaatca ctttctttaa atcaagtccg
    28801 tttttttgtg catagacaag catttccgtc attcctgtca tcgtacctgc aatcatgatc
    28861 tgattagcca tttttgtatg ctgacctttc ccagcagaac catgcaatgt aaatgtcgta
    28921 ccaaattctt tgaataaagg cagtacttga tcataaacgg cttcttctcc accgaccatg
    28981 atcgtcagtg taccattctt tgctcctaag tcaccaccgg ataccggtgc atctaacgca
    29041 tctgcgcctt tttcttttgc tgtttttgca attttttctg ccaggcttgg tgtacttgta
    29101 gtcaaatcaa ctaaaatatg tcctgagata tctgcagaaa aaatccccga ctgtccatag
    29161 tagatttctt ctacatcttt ggggtaacct accattgtaa agagaatatt actttgttct
    29221 gctaccgctt ttggtgtatc tgcccagata gctccttctg ttactaaagg atcagctttt
    29281 gcttttgtcc gattatagac agctacttcg tgtcctgctt ctagtagatg gcgtgcgaca
    29341 gcacttccca tgacgcctgt tccgataaaa ccaagtttca ttttttcgac ctcctaaaaa
    29401 tcaaaaatcc ttgcttctat gctagtatac gctagaaaca aggatttttt cataaaaaat
    29461 gtttgaattt tatcttttaa atgaatttaa ttttattttg attcgttcaa tcgagtcttc
    29521 ttcttctttt cttcgcatct cttcttcatg ataaacaacc atgtcttgat aaaattcgat
    29581 catagcttcg ccgaaatgtg tagatgaaat atcataaagt ttagcttcta aaacttttgg
    29641 atctttagga acttcactgg aaatataatc aataagtgct ggtgcaaagt cttcgtcggt
    29701 ttcaaaagtt ttccctaatg aaggatgatc gaacaatcgg ttcaaataag catttccttc
    29761 cacgacacaa ggtgttccgg ctgccatcgc ttctgtataa gtaagccctt gtgtttctga
    29821 agtagaagca ctgacaaaat aatctgccgc tttataataa agtgctacat cattgttgtc
    29881 cacttctcct gtgaactgca caaattcttc taactgcagt tctgagatca attcctctaa
    29941 ctcttctaca taaggtcctt ttcctacgat aactaatctg gcgttaggat aagcggagat
    30001 gacctgagga aacccattaa tcaaggcttg gatatttttc tcataagaaa tcctgcttaa
    30061 agaaagaatc atcaaatggt tttcctgaag acctagtgac tcccttaatt tgctgatatc
    30121 ctgctgagtg atatcagaac gtttgaattt ttcaatgtcg ataccagtag gaatcacgcg
    30181 aagcggtgct attacaccat agctgcgtaa tgtatcaata acacgttcac ttggacaaac
    30241 tacacctgtc gtatgatttg caaataatct agacaagtat tttacatggg attgcctgat
    30301 cacttttcct ttaccgatat aatgaaggta gtcttcatac atcgtatggt aagtatggat
    30361 cagaggtatt ttcaacttct tagctaccat cttcccaagt atccctgctc cgaattctgt
    30421 atgggtatga ataagatcta gttctagttc tttggcaacc aaataagcat accacatgcc
    30481 tctaacaaca actcttcgat ccttgaaaga aataaatggt acacttggca ttcgaatcac
    30541 atctttttcc aacttatccg cattaggatc ggtcgttgta aaaatataga cttcatgccc
    30601 atgtctttct aattcttgtt ttaatgtttt gatcgatgta gcaactccgc tgacttgcgg
    30661 aaagtaggta tcggtaaaaa agccgatttt catgtttttc gcctccaaac tagtccttga
    30721 cctgatttac tttttttaat ttgctagaga ctttttcatt cagtacactt tcgtatacat
    30781 ttttaagttc ttcaccgatt ttattgatac ttcgctcttt tgctgtttga taaccggctt
    30841 gtgtcgtact cggtagttct tgattggtaa tctgttggat cagtcggata aattcttcgt
    30901 ttgtcttccc catatagcag ttcttgctat cttctaacca gccattatac actggaatat
    30961 ctcgaacaag tacgttttgt ttgcttgcta aagcttctaa aacaacgatc ccttctgtct
    31021 cttcataaga agggaaaaag aatagatctg cacctgaata cgcaccttct atgatctctc
    31081 cacgaatgta accaggaaac aggacatttt caggatgatc ttcttttaca attttccgga
    31141 tgttccgagg aatcgagtac atgggaacat gaccaaacca aataaatgtg tattcgggca
    31201 tcttcttagc aatttctacg aaatcaatga ttcctttacg ttcgaaaaac aaaccgacac
    31261 aaatgattac tttttgatcc ggagacagtt tgaaatattc tctgaacttt tgttctttca
    31321 ttggatcagg gtgatatttc tctaagtcga tcccattgga aatagcttga atcggcacac
    31381 ggataccata gctttccaac aactgttttg aataaggtgt tggcgtgatc agatagtccg
    31441 ctttttggta caatcctacc agatattttt ttacgatagg agaaagctgg ttcgatccga
    31501 taaaggaatt tcggaaatct tcctctgtag aatgcgcatg atagacaact ttttttcctt
    31561 ctcttctggc tttattgacc atattatgac tattgattcc gtatgtattg atgtgcagga
    31621 tgtcataatc ttctttttca tctagtgtat aagagatacc gacttccgtc agagcacgtt
    31681 tttgatgatc aagcgcacgc ccgattccag attttgcaag catcttttca ctttcaaaat
    31741 ataataatac cttcacctta cgtccccctt cacttatata gtataacata gcctacaaac
    31801 tgtcactcaa acttaagccc tatttttgtc tattcttact gacaaaaacg ttttcttaaa
    31861 aaagaacaaa aaaactggcg tagctctttt acaagccaca ccagcttttg atacgttgtt
    31921 tattttacat attcatgaac aagttcaacg acttcttcca tcatatcgca ttctttcaat
    31981 gcacgatcag caagttcagc cattttcgct gtgtccaagc gtttcattaa actacgcgtt
    32041 ttaagaatag atgtcgcact cattgagaac tcatctagac ccatacctac tagcaatgga
    32101 acagctgttt gatctccagc catttctcca cacatacctg cccattttcc ttcagcgtgt
    32161 gctgcatcaa ttacattttt gatcaaacgt aaaattgatg ggttatatgg ttggtacaag
    32221 taagaaacgc gttcgttcat acggtctgct gccattgtgt attgaatcaa atcgttcgta
    32281 cctacagaga agaaatctac ttcttttgca aacttgtctg ctaagactgc tgctgctgga
    32341 atctcgatca tgatgccgac ttggatcgta tcagatactt ccttgccttc acttatcaat
    32401 ttttgttttt cttcttcaaa gatcgctttt gctgcgcgga attctttgag tgtagctacc
    32461 atagggaaca tgatacgtaa attaccatga acagatgcac gaagcaatgc acgcatttgt
    32521 gtacggaaca tttcgtcgcc ttgttcagat aaactgatac gcaatgcacg atatcctaag
    32581 aatggattca tttcatgagg taattgcaga tatggtagtt ctttatctcc accgatgtcc
    32641 attgtacgaa caactacagg ttttccttcc ataccttcta atacagcttt gtaagcttca
    32701 tattggtcat cttctgttgg gaagtctgga gaatccatat acaggaattc agtacggtac
    32761 agaccaactg cttctccacc attattatgg acaccaacta gatcttttgg tgttccgatg
    32821 tttgcagcta attcaaaatg cttgccgtca gctgtgacag tttctgcatt ttttaatttt
    32881 tcccattctg ctttttgtgc tgcgtaatct gcacctgctt tttcaaattc agctttttgt
    32941 tcatctgtag gatcgatgat tacatcgcct tcgatcccgt tgacagccaa aatatcgcct
    33001 tctttgactt tagcagtaat ttcttttgtc ccaacgattg ctggaatttc taaagaacgt
    33061 gccatgatag cagaatgtga agtacgtcca ccaatgtctg ttacaaacgc ttttacaaaa
    33121 ttgcgatcta attgtgctgt atcacttgga gtcaagtcat gtgcaacaac gaccacttct
    33181 tcgttgatca ttgatggatt tggtaaagta acgcctaata agtgcgctaa aatacgttta
    33241 gcaacgtcac gaatatctgc agcacgctct tgcatataag cattatcttc cattgcttcg
    33301 aacataccga tatacatatc cgttacttct tttagcgctg attcagcatt tacgctgttg
    33361 tctttaatat tttgcttgat ttgacctacc atttctggat ctgaaagaac catcaaatgg
    33421 gcatcaaata cttgcgcttc tgcttcacct aagctttgcg ctgctttttc acgaatttgc
    33481 tgcaattctt cagtagattt agctaatgca gcatccaaac gagcttcttc agccgcagta
    33541 tcttctactg ttgccttact gaatgttaaa tccggttgaa ctagcaggta agcttttgca
    33601 acggctacac cgtcactagc ggcaatccct tttagcattt caaccattat tcagataagc
    33661 cttctttctt cattgtttca acgattgctg ccattgcatc agcttcgtca gcaccttctg
    33721 cagtaatagt aacatcagaa ccttgaccta cacctaaaga cataacgccc atgattgatt
    33781 tcaagtttac tgatttacct ttatactcta agtttacatc agagttaaat ttgcttgctg
    33841 tttgtactaa tagtgtagct ggacgagcgt ggatcccagt ttctgctact acgtgaaatt
    33901 ctttcttttc catattaatc ggtctccttt aagtgagtgt tgtttttatg tgagggtttt
    33961 cttttttagt aattcagaga atcctaacaa atgccaccct ttcatctgta aagattaaca
    34021 ttttttttcg ttagatacaa cagttttcat tcaatttcag ataaaagtat tcaaattatt
    34081 ccggattctt tttcatcctt ttcttcacat tttataacaa acgtgtttat ttcctttttc
    34141 ttgtcgcttt ctcttctcct gacgtttatc attctttcct ttgaccctct ttgttttcat
    34201 atgaaacgaa ttacttgctt ccctttttat ttttagtata ataagagtca aaggtcagga
    34261 atggtcaata gagatttcac cttttctacc tcataaatct cttaaaaaac taggggttat
    34321 tcttttacct catgaaaaac gagatgtaaa atatggatgt acccattttt gtgaaaggac
    34381 gtgaatgatt catgctttgt caaaactgcg gcaagaatga ggcaacgatt cacttgtatg
    34441 ccactgtcaa tggtcagcgg acacaactcg attactgtca gagctgttat caaaaaatca
    34501 aaaatcaaca aaatggaggt ctgactaata tggcccaaaa tgatccattt ggatttggca
    34561 gtttggacga tcttttccgt tccatgtctc gccaaatgca acagcaagga aattatgagc
    34621 aaacaccacc aacacaattt ggcggaaaca acaacttcaa tggtggtcag ccacctcaag
    34681 gcaatacaga aggtttattg ggagaatacg gaatcaatat cacagaacaa gcacgacaag
    34741 gtgatatcga tcctgtgatc ggacgcgatg acgaaatcaa acgagtcatt gaaattttaa
    34801 atcgccgaac caagaacaat cctgtcttga ttggagaacc aggtgttggt aaaacagcag
    34861 ttgttgaagg tttagcacaa aaaatcgtag atggtgatgt accgcaaaaa ttaatggaca
    34921 aagaagtcat tcgtttagat gtcgtatctt tagtccaagg tactggtata cgaggacaat
    34981 ttgaagaacg tatgcaaaaa ttgattgaag agatccgtca agcagaaaat gtgatcctat
    35041 tcatagatga agttcacgaa atcgtcggtg ctggttctgc tggtgatgga aatatggatg
    35101 ctggtaatat tttaaaacca gctttagctc gaggagaatt gcaaatggtt ggtgctacta
    35161 ccttaaatga gtaccgcatc atcgaaaaag acgctgcttt agaacgtcgt atgcaacctg
    35221 tacgggtaga tgaaccaact gtcgaagaaa cgatcagtat tttgaaaggt ttgcaaaaac
    35281 gttatgaaga ctatcatcat gtaaaatata ctgatgaggc catcgaagca gccgcaactt
    35341 tatccaatcg ttacgtccaa gatcgcttct tgccggataa agccatcgat ttgctagatg
    35401 aaacaggttc taagaaaaac ttgacgatcc aaatcgttga tcctaaaaca atcgagaaaa
    35461 aactgcaaga agcagaagaa caaaaagttt tagcttctcg agaagaagat ttcgaaaaag
    35521 cagcttatta tcgcgatcag atcaataaac ttcaaaaaat gaaagaacgt cagctcactg
    35581 aagaggaaac acctgtgatt accgaaaaag atatggaaaa aatcgttgaa caacggacag
    35641 gtattccagt gggtgaatta aaagaaaaag aacagacaca actgaaaaac ttagcagatg
    35701 acttgaaagc tcatgtcatc ggacaagata acgctgtaga tcgagtagcg aaagcgattc
    35761 gccgaaaccg tgttggatta aacaaacaga atcgtccaat cggctccttc ttatttgttg
    35821 gaccaactgg tgtaggtaaa acagagcttg cgaaacaact tgcctacgaa ctattcggtt
    35881 cacaagattc aatgattcgt ttcgatatgt ctgaatatat ggaaaaacat agtgtctcta
    35941 aattgattgg ttctcctcca ggttatgtcg gatacgaaga agctggtcaa ttaacagaaa
    36001 aagtacggag aaatccttac agtcttgtct tgttagatga agtagaaaaa gctcatccag
    36061 atgttttgca tatgttcttg caaatcttag atgatggtcg attgacagat gcacaaggaa
    36121 gaacggtcag ctttaaagat acgatcatca ttatgacaag taatgccgga actggtaaag
    36181 tagaagcaaa cgttggtttt ggtgcggcac gcgaaggtgt tactcgttct gtattgaacc
    36241 aattaaacaa ctactttaca ccagaatttc tgaaccgttt tgacgggatc atcgaattca
    36301 gtgcattatc aaaagaaaac ttgatgacaa tcgtcacact aatgctagac gatgtcaacc
    36361 aaatgctggc agcacagcaa cttcacatcg aagttcctac gaatgtcaaa gaaaaattag
    36421 ttgacttagg ctatgatcca tctatgggtg ctcgtcctct tcgacgcacg atccaagaac
    36481 aaatcgaaga tggtatcgct gaattttatc ttgatcaccc ttctatccat gagttgaaag
    36541 caaaactgga taaagatgga aaaatcatcg ttacttctaa accagaacgt ttggctaaag
    36601 aatcagccga agaaacggct gaataaatcc agttttatag cgtgagacgg tatttctttg
    36661 agaaatgccg tctcattctt ttttcagaaa agtcaaaata ctgttcattt tataaaaaag
    36721 aaattataat aagagaaaca agcaattaaa aatacataaa agaggtgagc gccatgaaat
    36781 tggtaaatgt aacaaacagc cattcccgtc ttgtattaaa ccagcttgaa aacactgatg
    36841 cccatctagt caaggtctac acagccggaa ataccactat tgtctacact gaagcccctg
    36901 aacataatga aattctgctg atcaatgaca aacgtaaaat ccagccgaaa gaaatcgaag
    36961 atgctaaaaa ttatttctta gataaaatca aagacgccgt ttaccacgag gatgaaatca
    37021 aagtaatcga attggcaggc cttgttgaaa tctcgattcc aaaacatgaa ttaggcgcta
    37081 cattagccgt gtaaacaaat tttgaacata at
//
LOCUS       DACEUZ010000011        35438 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.011, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000011 DACEUZ010000000
VERSION     DACEUZ010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35438
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.011"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            <1..1152
                     /locus_tag="IXG44_000486"
     CDS             <1..1152
                     /locus_tag="IXG44_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005236379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1657226.1"
                     /translation="PSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSA
                     EAPKEKVETSGSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKED
                     IENFLAGGPSSAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPT
                     RKAIAKAMVNSKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALT
                     ATVKKYPVLNASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEI
                     NEKAKLAHDGKLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIV
                     NAEGEIVVGRVMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            1160..2566
                     /gene="lpdA"
                     /locus_tag="IXG44_000487"
     CDS             1160..2566
                     /gene="lpdA"
                     /locus_tag="IXG44_000487"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HAQ1657227.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            2728..3003
                     /locus_tag="IXG44_000488"
     CDS             2728..3003
                     /locus_tag="IXG44_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HAQ1657228.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            3009..3776
                     /locus_tag="IXG44_000489"
     CDS             3009..3776
                     /locus_tag="IXG44_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HAQ1657229.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            3944..5773
                     /gene="typA"
                     /locus_tag="IXG44_000490"
     CDS             3944..5773
                     /gene="typA"
                     /locus_tag="IXG44_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HAQ1657230.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            6034..7806
                     /gene="recQ"
                     /locus_tag="IXG44_000491"
     CDS             6034..7806
                     /gene="recQ"
                     /locus_tag="IXG44_000491"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HAQ1657231.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            7920..8222
                     /locus_tag="IXG44_000492"
     CDS             7920..8222
                     /locus_tag="IXG44_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HAQ1657232.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            8225..9388
                     /locus_tag="IXG44_000493"
     CDS             8225..9388
                     /locus_tag="IXG44_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAQ1657233.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            9414..12842
                     /locus_tag="IXG44_000494"
     CDS             9414..12842
                     /locus_tag="IXG44_000494"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HAQ1657234.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            12863..13987
                     /locus_tag="IXG44_000495"
     CDS             12863..13987
                     /locus_tag="IXG44_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1657235.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            13977..14414
                     /locus_tag="IXG44_000496"
     CDS             13977..14414
                     /locus_tag="IXG44_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAQ1657236.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            14398..14736
                     /locus_tag="IXG44_000497"
     CDS             14398..14736
                     /locus_tag="IXG44_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HAQ1657237.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            14855..15415
                     /gene="rsmD"
                     /locus_tag="IXG44_000498"
     CDS             14855..15415
                     /gene="rsmD"
                     /locus_tag="IXG44_000498"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HAQ1657238.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            15412..15903
                     /gene="coaD"
                     /locus_tag="IXG44_000499"
     CDS             15412..15903
                     /gene="coaD"
                     /locus_tag="IXG44_000499"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HAQ1657239.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            15893..16936
                     /locus_tag="IXG44_000500"
     CDS             15893..16936
                     /locus_tag="IXG44_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAQ1657240.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            17095..17823
                     /locus_tag="IXG44_000501"
     CDS             17095..17823
                     /locus_tag="IXG44_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComEA"
                     /protein_id="HAQ1657241.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            17939..18184
                     /locus_tag="IXG44_000502"
     CDS             17939..18184
                     /locus_tag="IXG44_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAQ1657242.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            18181..18399
                     /locus_tag="IXG44_000503"
     CDS             18181..18399
                     /locus_tag="IXG44_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAQ1657243.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            18535..19074
                     /locus_tag="IXG44_000504"
     CDS             18535..19074
                     /locus_tag="IXG44_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HAQ1657244.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            19067..21340
                     /locus_tag="IXG44_000505"
     CDS             19067..21340
                     /locus_tag="IXG44_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HAQ1657245.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            21378..22424
                     /gene="holA"
                     /locus_tag="IXG44_000506"
     CDS             21378..22424
                     /gene="holA"
                     /locus_tag="IXG44_000506"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HAQ1657246.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(22513..22767)
                     /locus_tag="IXG44_000507"
     CDS             complement(22513..22767)
                     /locus_tag="IXG44_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HAQ1657247.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(23167..23436)
                     /locus_tag="IXG44_000508"
     CDS             complement(23167..23436)
                     /locus_tag="IXG44_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1657248.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(23906..24361)
                     /locus_tag="IXG44_000509"
     CDS             complement(23906..24361)
                     /locus_tag="IXG44_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HAQ1657249.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(24499..26427)
                     /locus_tag="IXG44_000510"
     CDS             complement(24499..26427)
                     /locus_tag="IXG44_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAQ1657250.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(26624..27457)
                     /locus_tag="IXG44_000511"
     CDS             complement(26624..27457)
                     /locus_tag="IXG44_000511"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAQ1657251.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(27649..28353)
                     /locus_tag="IXG44_000512"
     CDS             complement(27649..28353)
                     /locus_tag="IXG44_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ1657252.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(28376..29002)
                     /locus_tag="IXG44_000513"
     CDS             complement(28376..29002)
                     /locus_tag="IXG44_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAQ1657253.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            29328..31703
                     /locus_tag="IXG44_000514"
     CDS             29328..31703
                     /locus_tag="IXG44_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HAQ1657254.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(31727..32218)
                     /locus_tag="IXG44_000515"
     CDS             complement(31727..32218)
                     /locus_tag="IXG44_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HAQ1657255.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            32405..33325
                     /locus_tag="IXG44_000516"
     CDS             32405..33325
                     /locus_tag="IXG44_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ1657256.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            33479..33823
                     /locus_tag="IXG44_000517"
     CDS             33479..33823
                     /locus_tag="IXG44_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HAQ1657257.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(34013..34513)
                     /locus_tag="IXG44_000518"
     CDS             complement(34013..34513)
                     /locus_tag="IXG44_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1657258.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            35041..35124
                     /locus_tag="IXG44_000519"
     tRNA            35041..35124
                     /locus_tag="IXG44_000519"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35075..35077,aa:Leu,seq:cag)
BASE COUNT        11775 a         6170 c         7296 g        10197 t
ORIGIN      
        1 ccgtcacctg tcacagggac agttaaaaac gtgattgttc cagaaggcac tgtagcaaat
       61 gttggggatg tattagtaga aatcgatgct cctggacaca atagtgcacc gtcaactagt
      121 gcgccatcag ctgaagcacc aaaagaaaaa gtggaaacat caggttcagc aagtgtcgtt
      181 gaagctgccg atccaaataa acgtgtattg gctatgccat ctgttcgtca atttgcccgt
      241 gaaaaagatg tagatatctc acaagtaact gcaactggaa aaggtggcag agtaacaaaa
      301 gaggacatcg aaaacttctt ggcaggtggt ccaagcagtg caccagcgaa atcagaagcg
      361 ccagaagcgg cagcaccgaa agaagcagct cctgcagcag aaagcaaacc agctgcacca
      421 gcaaaaccat tcaagtccaa cttaggagac ttggaagaac gtgtagcaat gacgccaaca
      481 cgtaaagcta tcgctaaagc aatggtcaac agtaagcata cagctccaca cgtgacattg
      541 catgatgaag tagaagtgtc taaactatgg gataatcgga aacgatttaa agaagttgct
      601 gctgcaaatg gtacgaaact tactttcttg ccatatgtcg tgaaagcttt aacagcgact
      661 gttaagaaat acccagtatt gaatgcatct atcgacgatg caaaccaaga aatcgtttat
      721 aaacattact acaatatcgg tattgcaact gatacggatc acggactata tgtaccaaat
      781 gtgaaagatg cagaccgcaa aggaatgttt gctattgcag acgaaatcaa tgagaaagca
      841 aaattagctc atgacggcaa actatctgca gaagatatgc gcaatggaac aattacgatc
      901 agcaatatcg gatctgtagg tggcggttgg tttacaccag tcatcaacta tccagaagta
      961 gctattttag gtgtaggaac gattgcacaa caaccaatcg taaatgctga aggcgaaatc
     1021 gttgtcggtc gtgtgatgaa actgtcatta agtttcgatc accgtatcgt agatggagca
     1081 actgcgcaac aagcaatgaa taatatcaaa cgtcttttag ctgatccaga gctattaatg
     1141 atggaaggat gataaacaaa tggtagttgg agattttgct gtagaattag atacagttgt
     1201 aattggtgcg ggacctggtg gctatgtagc tgccatccgc gctgctgaaa tgggacaaaa
     1261 agtagcaatc attgaaagag agtatattgg cggtgtttgt ttgaatgtcg gctgtattcc
     1321 ttcaaaagca ctgatagctg caggacacca ttatcaagag tcactagatt ctgaaatgtt
     1381 tggagtaact agtgaaaacg tcaaactaga ttttgcaaaa acacaagaat ggaaagaaaa
     1441 caaagttgtc catacgttga cttctggtgt tggtttccta ttaaaaaaac ataaggttga
     1501 aacaatcgaa ggagaagcgt tcttcgtaga tgatcacaca ctacgcgtca ttcatccaga
     1561 ttcagctcaa acttattcgt tcaataacgc aatcatcgca acaggttccc gtccaatcga
     1621 gattccagga tttaaattcg gaggacgtgt gttagattct actggcggat tagcattgaa
     1681 agaagtacct aagaaatttg tgatcatcgg tggcggtgtg attggtgccg aattgggcgg
     1741 agcgtacgca aacttaggag cagaagtaac gattcttgaa ggctcaccac aaattttacc
     1801 aacttacgaa aaagatctag taaaattagt agaagatgat ttcaagaaaa aaggcgtaac
     1861 agtcgtaacg aatgcaatgg cgaaagaagc agtcgataac ggtgacagcg tgacagtgaa
     1921 atatgcagtg gacgggaaag aagaatccgt tactgctgat tatgtgatgg tcactgtagg
     1981 tcgtcgtccg aatacagatg atatgggctt agaacaagcg ggtgtcgaag taggagaacg
     2041 aggattgatc acggtggata aacaaggacg tacgaacgta cctaacatct atgccatcgg
     2101 tgatatcgtt ccaggggcgg cattagcaca caaagccagt tatgaagcaa aaatcgctgc
     2161 tgaagcaatc tctggcaaaa aagtagctgt agattataaa gcaatgccgg cagtagcctt
     2221 cactgatcct gaattggctt ctgtaggtat gacgatcaaa gaagcaaaag atgctggtat
     2281 cgaagcaaaa gcatataaat tcccattttc agggaacggt cgggcattat cgcttggtaa
     2341 aacagaagga ttcatccgtc tagttacgac gatcgaagat aacgtgctta ttggcgcgca
     2401 aatcggtgga gtcggcgcaa gtgatatggt ctctgaatta gctttagcaa tcgaatcagg
     2461 aatgaatgct gaagatatcg ccttgacgat tcatcctcat ccatctcttg gcgaaatcgt
     2521 catggatgct tcggaattag cattaggttt accaatccat atttgatttg aataatacag
     2581 gcgaaacgca actgaaaaat cagttgcgtt ttgtttttta gaattatcaa taatattatt
     2641 atgtaaactt aaaaatatga caacttcttg gtttattacc acttcctatg ctacactgaa
     2701 gaggaatatg aaataggaga gttgaagatg caagaatatc agtatccact tgatttagat
     2761 tggacaacag acgaaatggt cattgtaatg aagatgtggg aagatctgga acgtgcgaat
     2821 gaaaaaggga taaacaatga agcttttttg aagacttatc agcagtttaa aactgttgtc
     2881 aaatccattg gtgaggaaaa acgattggga cgggaatttg aaaaagcttc cggttattct
     2941 ttatatcgta cagtgcagca agcaaaaaaa aatccaaaca aaactttgag aatgaacggg
     3001 tgagataaat ggaacagatg attgatcata tcaagtcctg gcttgaagaa gctggtgaga
     3061 aaatcaaatc ttctcaacaa acattaacaa tctcgcaaaa atcaaatcga acggatctag
     3121 taacaaatgt cgatcgaaac atccagaatt ttctaatcga aaaaatccat tcctttgatc
     3181 cagaggcgaa aatattggca gaagaagaag ggtgtaatca attatcggat ctcgatggaa
     3241 gagtctttat catcgatcct attgatggca ccttgaattt tgtgattgaa ggggaaaatt
     3301 tttgtattat gctcgctgtt tatgaatccg gcgttggaaa gcttggtttc atttatgacg
     3361 tgatgagaaa tgaacttttt tggggtggaa aaggaatagg cgtgtataaa aatgatgagc
     3421 cattgcctag accagcaaat aaatccttat cggaaggctt acttggtgtg aatagttatc
     3481 ttttcggtca taataaatat ggaatccgag aaattgcgga aagcagcatg gggacacgta
     3541 tgtacggatg cgctggtctt gaactagtgg caatcttgaa aggaaaccac attgcttata
     3601 tttcaaacct tagcccttgg gattatgcag cgggaaatgt cttattggaa gagtttggta
     3661 tggtaacaag cggtcttttt ggtactcctt tgacatttga aaaaagggaa tactatttag
     3721 cagggacttc ttgtgcatat gcagaaatcg cagacctttt gaaaaaagaa ggctgaaaaa
     3781 aaggtagaaa tcattcacaa aaatgataaa aacgagcaaa agattaattt tctcgaaaaa
     3841 atgaaaacat tttactatat ttcatgaaaa aagtctgcta taattagcag tcgagtaaaa
     3901 tttgccctgt cataaagaac caataaaaaa ggagttagtt acattgaatt atagaaacga
     3961 tatccgtaac gtcgcaatta ttgcccacgt tgaccacgga aaaacaacat tagttgatga
     4021 actattaaaa caatcagata cattagacgc acatacgcaa ttgcaagaac gtgcgatgga
     4081 ctcaaatgcg ctagaaaaag aacgtggtat cacgatttta gccaaaaaca cagctgtaga
     4141 ctataaaggt attcgcgtaa acatcatgga tactccagga cacgcggact tcggtggcga
     4201 agtagaacgg atcatgaaaa tggttgacgg cgttgtgttg gtcgtagatg cgtatgaagg
     4261 aacaatgcct caaacacgtt ttgtattgaa aaaagcactg gaacaacata tcacaccaat
     4321 cgttgtcgta aacaagattg acaaaccttc tgctcgtcct gagcatgtcg tagatgaagt
     4381 tcttgaacta ttcatcgaat taggagcaga tgatgatcag ttagactttc ctgtgattta
     4441 tgcatctgca ctaaatggta cgtcaagctt gtcagatgat ccagcagatc aagaaccaac
     4501 catggcacct atttttgata caattattga aaaaatccct gctcctgtcg ataacagcga
     4561 cgaaccatta caattccaag tttcattact tgactacaac gattatgtag gtcgtatcgg
     4621 aatcggacgt gtatttagag gaacaatcaa agtaggagac caagtagcat taattaaatt
     4681 agacggaaca gttaagaaat tccgtgtaac gaaattattt ggtttctttg gactaaaacg
     4741 tctagagatt caagaagcaa aagctggaga cttgattgct gtctcaggta tggaagatat
     4801 ctttgtcggt gaaacagtta ctcctgtgga tcatcaagat gcgttaccta tccttcacat
     4861 tgacgaacca acattgcaaa tgactttctt agttaataat tcaccatttg ccggtcgaga
     4921 aggtaaattc gtaaccgctc gtaagattga agaacgtttg atggcagaac ttcaaacaga
     4981 tgtatcctta cgagttgaac caacaaactc tccagatgca tggacagttt ctggtcgtgg
     5041 agaattgcat ttgtctatct tgattgaaaa catgcgccgt gagggatacg aattgcaagt
     5101 atctcgtcca gaagttattg aaaaagaaat cgacggtgta aaatgcgaac cgtttgaacg
     5161 cgtgcaaatc gatacaccag aagaatatat gggtagtgtt atcgagtcct tgagcttacg
     5221 taaaggtgaa atgcaagata tggtccatac aggaaacgga caaatccgtt tgacattctt
     5281 aacacctgcc cgcggtttga ttggttattc aactgaattc ctttcaatga cacgtggtta
     5341 cggaatcatg aaccatactt ttgaccaata tctgccaatg cttcctggtc aaattggcgg
     5401 acgtcaccaa ggggcgcttg tttcgatcga tacaggaaaa gcaacgactt attctatcat
     5461 gagtatcgaa gaacgcggta cagtcttcgt agaaccaggt acagaagtat atgaaggaat
     5521 gattatcggg gaaaacagcc gtgataatga tttgactgtc aacatcacga aagcaaaaca
     5581 aatgacaaac gtacggtctg ctactaaaga ccaaacatct gtcatcaaaa aaccaaaaca
     5641 attgacgttg gaagaatctt tggaattctt gaatgatgat gaatattgcg aagtaacacc
     5701 agaaagcatt cgcttgagaa aacaaatttt agagaaaaat gctcgtgaaa aagcatctaa
     5761 aaagaaaaaa taatctgtaa aaggatgaag agagaaacca gcttgttata aaaaatagct
     5821 ggtttctttt tttgtccaac tcttttctgt ggagaagtca tctttgtcaa tggacataaa
     5881 ttcagaaaat acgccgaaag acgtttaaaa gacttttaaa aaacttattt ctagagagtt
     5941 tttgtttcat aataagataa tgtttcacga aaggagatca acttgtcctt cgaagaaaat
     6001 cgaagtaaaa tggcaagaga aaggagcggg aagatgaacg acttattaaa acaatacttt
     6061 ggctatgatg agtttagacc tggtcaaaaa gaaattatcc agaaagtcat tgatcaggaa
     6121 aatgtattag gaatcatgcc taccggaagt gggaaatcaa tctgttacca gcttccagct
     6181 ttactattag atgggcttac tgtggttgtg tcccctttaa tctctttgat gaaggatcag
     6241 gtggacgcgg cgaatcaatt aggtataccc gccactttta tcaatagctc gttagatggt
     6301 tacgaaacag ctagacgctt ccaagaaatc gatagacagc agtaccgttt actttatatc
     6361 gctcctgaac gttttatcat gccggatttt atccaagcaa tgaagcgatg gaacgtccgt
     6421 atgatcgcta ttgacgaggc acattgtatc tctcagtggg ggcatgattt ccgcccaagt
     6481 tatttacaga tggcgaacca gctagatcaa ctgccaaatc gtcctgtaat tgttgctttg
     6541 acagctacag cgactgtgca agtagctgct gacataaaaa gacttttaaa aattccagaa
     6601 aacaaccata ttcagactgg ttttgaacga gaaaatctac gatttcaagt aattaaagat
     6661 caaaaaaaag aacaatatct aatcgaatat ttaaagatca acaaaaatca atctggtatc
     6721 atttatgcag caacgagaaa agaagtagac cgtctctatc atttgcttaa aaaattcgat
     6781 ttttccgtgg gtagatatca tggtggtcta aatgagaatg agcgaacaga aatgcaagaa
     6841 gcctttttat acgatcgctt gcagctgatt gtagcgacaa atgcatttgg tatgggtata
     6901 aataaaagca atgtccgttt tgttatccat tatcaaattc ctggctctct agaagcatac
     6961 tatcaggaag ctggtcgggc gggaagagat ggtctttcca gtgaagccat cctgcttttc
     7021 tctcctcaag atatccaagt ccaaaagttt tttatccagc agtcccaaag agaagaaggg
     7081 caaaagcaaa aagaatatga aaaattaaaa gcgatgacag aatacgtgta tattgagtcc
     7141 tgtcttcagc aatacatcct aaactatttt ggcgagacaa gcagtccgtg caaccgttgt
     7201 ggtaactgtt tagatgacag agaaatagta gaagtaacga cagaagcaca gatggttctt
     7261 tcttgcttaa aacgtatggg tgagaactat ggtaaacaaa tgctgatgaa agtcttagca
     7321 ggatcgaaag aacaaaagct gcaagcttta ggttttggac atttatctac ctatggactg
     7381 atgaaaaacc aatcgcaaaa agaaacgatg cagttaattg aatatttgat atcgaatggc
     7441 tatcttctga ccatcaatgg tgagtatcct gttttgaaag taacagaaag agggatacaa
     7501 gtattgaaag gacaagaatc tgtttatcga aaggaaccca agaaagtcca gcagctatcg
     7561 gatgaagaaa ccgatacact atttgaagtg ctgcgggaac tgcgaacgga tctagcatca
     7621 gaagcaggcg ttcccccgta tgtcgtattt tcagattcca cattgaaaga aatgagccga
     7681 atacgtccat caagcagatt ggagatgctt cagataaaag gagttggcca aagcaagctt
     7741 gataaatatg gcgaagcctt tttatctcgg ataaaaaatg cggactctaa cgttctggat
     7801 aagtagaaag aacgaatacg taaaaatgaa taaaaacaac tggttaaatc tgtttttttt
     7861 gctccttatg aaaatcaaat atgttataat taaggaatta gaatgggagg taagcacaga
     7921 tggattcttt gtcgaaaaaa cttgcgatcc aagtattaaa tgatgaagca gaacggatca
     7981 aacgattaat ccgaaatcaa aacaacagtc tctgtatctc acaatgcaaa gccttcgaag
     8041 aagtggtaga tacacagatg tacggctttt cccggcaagt cggttttgct gtccgtatag
     8101 gagtaatcgg aaagaacgag ggccagctga tgttaagtga gctagaacga gaattgaatc
     8161 agttatatag tgaggtttac caagaaattt atgataaaaa agaagttggc aaggaggaat
     8221 agacttgcct aagaaagtga aaaaaaggca tctattagac tatagcatct tgattcccta
     8281 tttaatttta tgtgtattgg gattgatcat ggtatatagt tctacgtcgt atctattatt
     8341 agaaaacgga caaaatccgt ccgcatcagt cattaaccag agtatttttt gggtgcttag
     8401 cttgattgct atcgcactac tttataaaat gaaaacagat gttttgaaaa accagcgttt
     8461 gatcatggca gccatcgcag ttttgacgat tttgctgctg attgttgtgt tttttggtaa
     8521 ggagatcaat ggggcgaaag gttggctgca aattgcagga ttctcgatcc aacctgccga
     8581 atacctaaaa atcatttcta tctggtacct gtctctgact ttatctaaaa gacaaaacag
     8641 tgtacaaaaa gattttcttg ggactgtgaa acgtccactt gcaatggtta tcggacttac
     8701 tgcattagtc gctattcttc ccgattttgg taatgcggca gttatctttt tgattatttt
     8761 ggttctgctt ttagccagcg gagtcaatta tgtgtatact ttgattgtag gagtaggagg
     8821 cttttgtcta agtactttta cgatctggct gatcaatatc acaaacggaa agatcttacc
     8881 cggacgttta cagtatatct ataatcgatt tgctatttac caaaatcctt tctcagacga
     8941 attaaataaa gggcatcaac tagtcaacgg ctactatgcc atgttcaacg gaggactatt
     9001 tggcagggga ttaggtaaca gtatccagaa aaaaggattt ttacaagaag cacaaaccga
     9061 ttttatttat gcaatcgtag tggaagaact gggagtgatt atggggattt tgatattagc
     9121 gttgctattc tttatgattg tgcgcattgt ccttgtaggt atccgttcaa aagatccgtt
     9181 caattcgctt ttgtgcattg ggatcggcgc aatgtttttg atccaagtat ttgtcaatct
     9241 tggaggaatt actggcgtta ttcctttgac agggatcacg ttcccatttt taagtcaagg
     9301 aggctccagt ttgctgatgc tttcgatttg tgtcggcttt gtcctcaaca tcagtgcgga
     9361 tgaaaaacgc aagagcttag gtttataata aaaaaagatg taggagtggt tcgatgaaaa
     9421 aagttttagt tgctaacagg ggtgaaatcg ctgtacgtgt atttcgtgct tgtacagaat
     9481 taggaattaa aactgttgga atctatgcag aagaagatga atattccgta caccgtttca
     9541 aagcggatga agcatattta gtaggacaag gaaaaaaacc gattgatgcg tatctagata
     9601 ttgaagggat catttctatc gcgaaagaat gcggagctga cgctattcat ccgggttacg
     9661 gtcttttgtc ggaaaacttg aattttgcgc aacggtgcga agaagaaggc atcatttttg
     9721 ttggacccaa actgcatcat ttagatattt ttggagataa aatcaaagca aaagctgcag
     9781 ctatcgaagc ggggattgct tcgattccag gcacagatgg accaatcgct tctatagacg
     9841 atgccttgga gtttgcgaaa caatacggtt atccgattat gatcaaggca gctctaggcg
     9901 gcggcggacg cggcatgcgt gtagctcacg atgaaaagag tgcaagagag ggctacgaac
     9961 gtgcgaaaag cgaagcaaaa gcagcctttg gtagtgatga agtgtatgtt gaaaaataca
    10021 tcgctaatcc taagcatatc gaagtacaga ttctcgggga tacgcatgga aacgtcatcc
    10081 atctttttga aagagattgt tctgttcagc gccgtcacca aaaagtagta gaagtagcgc
    10141 cttgtgtctc tatgaatgaa cagcaacgtc aaaaaatctg ccaggcagct gtccaattga
    10201 tgaaatatgt cgggtatgtc aacgcaggta cagtagaatt tcttgttgaa ggagatgatt
    10261 tttactttat cgaagtcaac cctcgcgtcc aagtggaaca tacgatcaca gaaatgatta
    10321 cagatattga tatcgtaaca acgcaactgt tgattgctca aggtttggat ctgcataaag
    10381 aaatcggatt gcctcagcaa gaaggaatta aattaaatgg ttcagccatt caatgccgaa
    10441 tcactacaga agacccgttg aataactttt taccagacac agggaaaatc gatacgtatc
    10501 gttcacctgg tggttttggc gtacgtctgg atgtcggaaa tgcttacgcg ggctatgtag
    10561 tgacaccgta ttttgattca ttgctagtaa aagtatgtac gcatggtgct acctttgaaa
    10621 cagccattca gaaaatggaa cgttgcttga aagaatttag aatccgtgga gtgaaaacaa
    10681 acattccatt tatgctcaat gtcatcacac atcctgaatt ccaatcagga aatgcaaaaa
    10741 caaccttcat tgatagtaca gcaacattat ttgaatttcc tcgactacga gaccgcggaa
    10801 acaaaacgat gaaatatatc ggtgaaatta ctgtgaatgg ttttccaggt atcgaaagtg
    10861 gcgaaaaacc attttatgaa gaaccacgta tgcctaaaga tctaatcaca agaactgatt
    10921 acgtaacggc taagaatgta ttagatgcga agggtgccga tgctttggtc gaatggatca
    10981 aaggacagga aaatttgttg ttgacggata cgacatttag agacgcccat caaagtctgc
    11041 tagcaactcg tgttcggaca aaggatttca agcagattgc acgtttgact ggagaaggtc
    11101 ttcctgaact cttttcaagt gagatgtggg ggggagcaac atttgatgtg gcttaccgct
    11161 ttttgaatga agatccttgg caacggttga gaaaaattcg ttctttaatg cctaatacgt
    11221 tgctgcaaat gcttttcaga ggttcaaatg ccgttgggta ttctaattat cctgacaatg
    11281 tattggtgga atttgtcaaa gaagcagcag cgcaaggaat cgatgtattt cgaatctttg
    11341 atagtttgaa ctggactcca caaatggaaa aaagtatcca agctgtacgg gatactggta
    11401 aaatcgcaga ggcagctatt tgctatactg gcgatatcaa cgatccaagt cgggcaaaat
    11461 acaatgttca gtattataaa gacatggcaa aggaactaga acaactgggt gcgcatatca
    11521 ttgcaatcaa agatatggct gggctgctta aaccgcaggc agcttatcgt ttgattagtg
    11581 aattgaaaga aacgacagat ctgccaatcc atctgcatac tcatgatacc agcgggaatg
    11641 gaatcatcac ttattctgct gcttcaaaag caggagtaga catcgttgac gtggcaatga
    11701 gtgcaatgag tgggaatact agtcagccta gcatgagcag cttgtattat gcactggtca
    11761 atggtccacg gctgccagaa atcacgattg aaaatgcaca gaaattaaac cattattggg
    11821 aagatgtgcg catgtattac aaaccgtttg aaaacggctt gaatgctcct gaaacagaag
    11881 tatatatgca tgaaatgcca ggaggacagt attcgaatct acagcaacag gcaaaagctg
    11941 tcggattagg tcatcgctgg gatgaaatca aacaaatgta tcatactgtc aacttgatgt
    12001 ttggcgatat cgttaaagta actccatcat ctaaagtagt tggagatatg gcactattta
    12061 tggtccaaaa tgatcttaca gaagaagata tttatgaaaa aggcgaaaca ctaagtttcc
    12121 ctgaatctgt tgtgactttc ttccaaggag aactagggca acccgttggc ggatttccta
    12181 aaaaattgca aaagattatt cttaaaggca gacctgcatt gaatgaacgt ccaggtcttt
    12241 tggctgaatc agtcgacttc aacgaagtaa aaaaagaatt ggcagaaaaa attggctatg
    12301 aaccaaaaca agacgaagta ttaagctatt tgatgtatcc gcaagtcttt ttggactatc
    12361 aaaaagcata taatcaattt ggcgatgtta cattactgga cacaccaaca tttttccaag
    12421 gtatacgttt gggtgaaaca atcaatgtac aaatcgaacg cggaaaaacg cttatcattc
    12481 gtttagatga aatcggagaa cccgatatcg aagggaatcg cgtactgttc ttcaatttga
    12541 atggacaaag acgtgaaatc actatcaaag ataattcgat catcagtacg gtccaaaccc
    12601 gattgaaagc tgaaccgaca aaccgagaac aaattggagc aacgatgtct ggttctgttt
    12661 tggatgtatt agtgaaaaaa ggcgataacg ttaaaaaagg tgatacgttg atgattactg
    12721 aagcgatgaa aatggaaacg gccatcgaag cacgttttga cggagaagtc gcacatgtat
    12781 atgtgtcttc aggagacact atttcgtctg gtgacctttt gattgaagtg actgagaaat
    12841 aataagcgaa aggtggggaa acatgaaaag actgggtttg ttttggctga ttctgctatt
    12901 agttattgtg gcattctaca tggaaccagt cttgtttccg ccaaaacttg aagtgaagga
    12961 aaactctgat caacaaatat caaaccaaat gacaacgtgg aaataccagg agcttaaagc
    13021 aactggtttt tctacttata tcggaaaacc gatcgatcag ctggaaaagg attttggcca
    13081 agcctatgat cgattaacta gcggatttgg ttttgaaaca agatattatg aaaataaagc
    13141 tagtcatctc acatttgaag cgaacatcca agacagcaga gtcacaactg ttaaagtact
    13201 gcaaacaggg cctgctgata tcgcgccgtt ttatttaggg atgacgatgc aagatcttac
    13261 gaaaatcact actatctata ctaatttcac ttttgagtac catgatacag aagttggaat
    13321 cgaactaatg gaagaggata tgaattatcg tccgctaatt gcttttgata acgacacttt
    13381 tgctatttta ttttttgatc aaacaacaaa cggcctattt tcagttgtgt atttagataa
    13441 agatagtctt ttgaaactat tgccatatca agtgtttggt gaaggccttc cacattatca
    13501 agcagataaa tcggctgatt gggaatcaat caatcgcgtt aaagaacgaa aaagtacgac
    13561 tttattaaat atgcttagaa gagaagacga acttcccata tacaatcaaa cacttgcctt
    13621 tcaaaaccac agtcagctgc tactacatga ttttttgaca aggcctgagg aaatattgtc
    13681 agaggaacgt atggctgatt ggcaaaaatc gctaggcagt gctcaaacga cagtgaaatt
    13741 ctcactgacg aatgatgaat tggagaagtt aatcagtaaa caaaaattag aaaatactag
    13801 tgggctattt attcatccgg cgattgacgc tactttttcg tttttatatt ggtacagcga
    13861 tccttacaac catgaccgtt tcatggaccc gggaacggat tctgtaggca ttgctttttc
    13921 taaagaaaat atgttagtct tattgcaaga agaaaacgga gaaagtagtg atagcagtga
    13981 tcgttaacga agagctgttt caactggagg atcaatgcct ccaagtcgct gaaatgataa
    14041 aaaagagtaa aacgatggct tcttatcttg aacataaaca gtcaatggaa gaatctgcaa
    14101 aagtttgcca gttgaaagaa gcgttcttgt ctaagaaaga agcatttgaa aatgtagcgg
    14161 catatggtga gtatgcacct gattatcgtg aaaaaaaacg agctgttcga aaagcaaaaa
    14221 gacagcttga tatggatgag catgttgcag ctttccgata tagtgaaaca cagctgcaaa
    14281 gtctgttgga tcaaattgtc caagtacttg ccgaagaagt ttctaatgaa atcaaagtag
    14341 atgcaggtag cccgtttttt gaaacgaaaa aacactttgg atgcggaggt aattgtcatg
    14401 caattagaca atagtgaatt tgaaataaca aaacgacgcg ggctgatcgt atgggtctac
    14461 agcttaaaac aattaaagaa tctgaaaaag ttcggcttgt tgcattatgt ctcaagaaga
    14521 atgaaatatg tcgtgcttta cttaaatgaa gagacattgg aagaaacaga agaacgaatc
    14581 cgtaaattac attttgttcg aaacgtagaa cgttcttacc gaccggatat cgaaatgaat
    14641 tttgccgaaa aaataggaaa caaaacagta ttaaaagacg aaggtttcga aattgaagag
    14701 ctaaatacag aaatccgatt agctgaacat ctttaaaaat ctcttatgag cagtttttaa
    14761 ataaaaaata acttggataa cgatcgattt ttgatcattg tccaagtatt tttttggtat
    14821 actagtacca gtaccatttc taaggagtga gtagatgcgg ataatatcag gaaattatgg
    14881 cgggcgccgt ttaaaaagtt tagcaggggc gaacaccaga ccaactacag ataaagtaaa
    14941 agaatccata ttcaatatga ttggaccata ttttgatgga gagacagtat tggatctgtt
    15001 tgcaggaagc ggtggtttag ctatcgaagc agtatctaga ggctgcagtc atgcggtatg
    15061 cgtagacaaa aattaccaag cgctaaaaat catcaaagaa aacatcgaaa ttacgaaaga
    15121 accagagaaa tttaccacaa taaaacttga tgcggacaaa gcaatcgaac ggctagcagc
    15181 ggagaaacaa caattcgact acttgtttct tgatccgcca tatgcaaaac aaaaaataat
    15241 cgatcaaatc cataaaatgg aagaactggg acttttttct aaaaccgcag ttatcgtatg
    15301 cgaaacggat aaaacagtcg aattgccaga aactgtggct ggattccatc aaataagaaa
    15361 acagatttat ggcataacgg ccgtaactat ctatagaaaa gagggaaatc aatgaaacgt
    15421 gcattattcc caggaagttt tgatcctttt acaaaaggtc acttagatac ggtggaacgt
    15481 gcggctaaat tattcgatga ggtagtaatt ggtgttttta tcaatacttc aaagaaaagt
    15541 ttgtttcccc cagaagaaag aatgacattg atcacaaaag ctgtttctca tcttcctaat
    15601 gtaaaagtca tgcatcaaga aaatcaattg acggtagaaa cagcaaaaga gattggtgca
    15661 gatgcgttga tcagaggcat acgaagtatc aaagactttg aatatgaacg agagattgct
    15721 cagatgaatc atcatctata tgatgaattg gaaactgtct tcttattagc aaaaccagaa
    15781 tatagtcacg tgagttccag tattttaaaa gaagtactgc actttggcgg agatgtgagc
    15841 agctacttac cgccagtgat caacgaagct ttagctggaa agcgtgaatc aaatgaaagc
    15901 taaacaatgg ttgaaaacag tactgattat catatttgga atgttgatca ttggatttgt
    15961 agtagttccc ttgccgtatt atgtagaatc accgggagcc acgatcgatc tgaaagaact
    16021 aattacggtc aatgataagg aagataaagg ttctgggtct ttctctctta cctctgttgg
    16081 tatcaggcaa gctacaggtt tcttagcttt aaaagctaaa atgacccctt ttgaagaatt
    16141 gatcagtgaa gaagaattga ctggtggggc gacaaatgaa gaatataatc aaatgcaaca
    16201 gtattatatg gattcttccc aaaatgcagc aatcgaacaa gcgttaaaac ttgcagacaa
    16261 gccttataaa atggaattca aaggcgtata tgtcatgagt gtagagaaaa catcgaattt
    16321 ctttggcaag ctttcagttg gagatacagt aacaaaagta gacgggaaaa gctttcaaag
    16381 tacagaagca tttatggatt atgtaaaaag ccaaaaagtt ggacaaacaa tcgaaatcag
    16441 ttatttgcat aatggtgaag aaaaaacagc ttctggtgat ctgatcgagt taccaacaga
    16501 taaaaaggca ggaataggta tcacgcttac tgaccatacc gagatcgaat cagatacaga
    16561 tgttcgtatt aattctggct ctatcggtgg gccatcagcg ggattgatgt tcactttaga
    16621 aatctatgaa caagtcactg gtaaaaactt gcgacatggt aagcaaattg caggaactgg
    16681 aacgatcaac agtgaaggag aagttggccg aatcggagga atcgataaaa aagtggcaag
    16741 tgcggataaa gctggcgtag agattttctt tgcaccagat gatacgattt cagacgatgt
    16801 taagaaagaa tatcctgaaa taaaaaccaa ctacgaagaa gcaaaagctg ccgctaaaaa
    16861 attgaattcc tcaatgaaaa tcgtaccagt aaaaacagta caagaagcgc ttgactatct
    16921 agagaaaatg aaatgaaact aaataattaa agaggggttg tgacacgagt tctgtcacaa
    16981 cccctctttt tcgtcaaaaa tcttcacgat aaaaataaaa acaaaagaaa aaattgacgt
    17041 aattagatgt tgaagggacg attttgccag agctcactgt ttcttcaatt ttaaatgaaa
    17101 ggagtaatga atgattttta ctggtcattc tttgaaaaaa gaacaatgat agaaaaaatc
    17161 agcaagaaaa tactagcttc acccaaattg atcatttgga tgtctatgat agttcttatc
    17221 ttttgtatcg caagtggttt tttattatca aatttgctat tttatagaga aacagaagag
    17281 actgctgcaa aagaaatact ttcttcgttt agttccagtg gagaagatcc atcagctgaa
    17341 ggaaacgatg tagaagagga tgctcgtcca aaggtcatgt acaccgatat caaaggaagt
    17401 gtgaaggagc ctggtatcta ttccttttct tcagaagaac gggtctatga tgtactaaag
    17461 cgtgcaggtg gattattaga agaagcggat agcgatcgaa tcaacttttc tgcgaagata
    17521 gaagatcagc aagtcctata tatccctgct gtaggtgaag agctgccaga acatcttgat
    17581 cagttggcat cctcggaagg aaaacagcct acagcagatt ccgagcctcc caaaataaat
    17641 atcaatacag ccactccatc agaacttcag cagattcctg ggatcggatc agtcaaagcg
    17701 caagaaatca ttcgatttag agaagaaaac ggatccttcc agaaagtgga agacctgcag
    17761 gagatttctg gtatcggtga aaagacagtt gaaaaactaa aaaacttcgt tacaattaaa
    17821 tagaatgatt caaaatcgac tggcacgagc aataaatgga cgctaatgga tactaagaat
    17881 aatccaattc taagagcttg gcaggacgag gaggtagtat ttttggatag gaaatagctt
    17941 gattgttcgc aaagaaacat taaaaaaacc aatgctgaat gtctatctcc aaaataaaat
    18001 atctgggatt catattatga atacggctgt cagcggcaat aacagtcaag ctctacgaga
    18061 acgctttgcc aaagatgtac ttagctatac agcagataaa gtatttattc tcattgggac
    18121 gaatgatcta gcagagcata aacaactttc taaagaaacg taccaaaaaa tctgcagtgg
    18181 atgatagttc agctgaaaaa agactatagt tctgatcatg ctttctttat cacgccacca
    18241 gcagtcgatg agacaaaaca aaaatatcgg aataacaagt tgatcaaaga atatatctct
    18301 gttctcacag aagtagcaaa acgccatgat tgtgaagtat cgatttttat acggaatttc
    18361 ttgaacaaat ggattcgaag aatttttata atgggctaat ggatgacgga cttcatttcg
    18421 gcagtgaagg gtaggatctt ttgtcagaca ggatgattcg ttatatttaa agaatatttt
    18481 gtttaagcaa tgagtacttt ttaatcaact tgaaaaaacg gaggaatata aaacatgtta
    18541 gaaaaatctt caaaagcatg cgatacgaat aaagaaaaag ttactgataa gtcacataat
    18601 gaacgcattc cttgggacca gtactttatg gctcaggcag tactgttatc tttacgtagt
    18661 acgtgtacac gtttagaagt aggagcgaca ctcgttcgag aaaaaaggat cattgctggc
    18721 ggctataatg gagcagtcag tggagatgtc cactgcatag atgaagggtg ttacgtcgtg
    18781 gatggacact gcttgcgaac gatccatgca gagatgaatg cactcctaca gtgtgcaaaa
    18841 cttggtatcc cgacggaagg ttcagaaatc tatgtcacac attttccatg tttggcctgt
    18901 acaaaggctt tgttgcaagc tggtgtgaaa gggatcaatt atctccatga ctatcgaaat
    18961 gatccttatg cccaagcgtt gatcgaacaa ttaggtgtat ctgtacacca agttgcttta
    19021 gactcacagc atttttcaaa acttcaaaat gaattaacag gaaaacatgt ctagcgtttt
    19081 agaaaggcat gttcgaaatc ggttgatttt tccggcaatt acgtcgatga ttatctgttt
    19141 gttgtattac actgacaatt ggggttatcg tttgcttctt gccggaattt ttttatggtt
    19201 ttcgctaaaa gaaggacgtt ttatcgcgta cttgtgtatt ttgtgtagca taacggtatc
    19261 catttcttat aggctacaag agtattcatt tttagaggaa aaaacaacat ctgcgcaaat
    19321 tactgtattt acagatacga ttcgtgcaaa tggtaatttt ttgacaatgg aaggaaggtt
    19381 atcagagaat aaacaaaagc tccagctttc ttatcaagta aacacagaag cagaattgaa
    19441 aacatggctt aacttccgag gaaagggaat agaattgact gctgaaggcg agttcatctc
    19501 tccagcaaaa aagcggaatc tttataattt tgaccaagaa gcttacttca ggaaccatag
    19561 aatttctgga cggttccaaa ttcaaaagat tgaacagatc accatgcaaa atcactggcg
    19621 aaactggctt cagagaaaaa gaggatcttt tatctccttt gtccaatctg aatttccgca
    19681 aaaaacaagt gtatatatta attctttatt atggggctat aaagatgcag gattcagaga
    19741 accagaagaa atttttaggg agagtggttt gctgcatctg ttcagtctat cgggtctaca
    19801 catccaattt tatttagggt ggctgtatta tttgtttcga cgtatcggat ggccactaca
    19861 cgtcagtatt attccgttga gtctactaac tatctgttat gtttctattg caggaagttc
    19921 tatttctgtt ttgcgtgcta gtttgctgtt tttattgcga ttgctagtca aattgctgaa
    19981 taagcattat tccaccatgg acttatttgc agttgtcttt tggcttcttt tactatttga
    20041 tcctcgattt ctttttcatg cgggtggaca gcttagtttt ttcatgagtt ttttgtttat
    20101 cctgatttct tttgatggag attggttgaa aaaaacggcc tctcacgtcc tgtttactat
    20161 gatcacgatg ccgttgattg tatggcattt ttatgagtgg cctattttag gtagtctttt
    20221 tacattactg attgcaccgg tattcaagtt tctttttctt cctggtgtgt tatttctcac
    20281 actcttcaat cgctggctgc cggaaacttt acttttgctg atagaagagg gattaatctt
    20341 atttgagcga ataatcgatt tttcttcagg gtcgtctctc attataggac gtttgcccct
    20401 tgtgctatct gggatgtata ttttcggatt actgatttgt atggataggt tgaaagaaca
    20461 acctgtgaaa cacttgtttt atgttggtct tttttctttt atttttttga ctttgccatt
    20521 tttacgcttt tcttcaacga tcgcttttat cgatgtcggt caaggagaca gtatcttttt
    20581 gcaaagtcct tttcgaaaag aaacgatttt gatcgatact ggaggtcagt tacactttga
    20641 aagagaaaag tgggcgcaag gtatgatacg tcctcctgct gaaagaaata tcgttccata
    20701 tctaaaaggt caggggataa gtgtaatcga taaactcatc ttgacacacg acgatacgga
    20761 tcatgtggga gaattaccca cgttatctca gcattttact atcaaaaaaa tttatatcgg
    20821 ttggggcgct ggacgaaatg agccattaca aaggcattta tctgaagctc aaaaaaatgg
    20881 tactgagatc attgaaatca aacatgggga caggatcagc ggttattatg atctttatgt
    20941 attgactcct tttgaaaagg gagagggaag aaatgaagat tcgattggtc tatggatgga
    21001 atataacaat aggcgctttt tatttttagg agatttgaat caagcaatgg aaaaacaatt
    21061 gcttttgatt tatcctaatc tgaaagccga tgtagtcaaa ttaggtcatc acggtagccg
    21121 tacgtcaagt aacacagatt ttctttccca tattgaagca aaacatggga ttatatcctg
    21181 cggcgtaaat aatcgttatg gtcacccgca cagcgaagta ttggagacac tagaagaaaa
    21241 tcagataaaa acatggcgaa cggatcaaca aggaatgatc acctatcgct ggcatccagt
    21301 ttttcatcct catgggctag tggaaacaat gatagactag taattctcat ctgctcatgg
    21361 taggataatt tttgaaagtg gtgagtaatt tgagtataca agcagagtta caaaaaatac
    21421 gtaaagaaaa acttgcatct tgttacttga ttttaggaac agaaaagttt ctccaagatc
    21481 aagttcggac agaaattgtc aaaaaaatcc aattagaggg cgaagatgac ttaaattttt
    21541 taagttttga catggaaaat gcaagtttag atgaggtagt agctgaagca gagacgttgc
    21601 cgtttttcgg agatcaacgg ttagtattcg tagagaatcc ttatttttta acaggtgaaa
    21661 aagtaaacaa tggaatcgaa caaaatactg atttattgac ggcttatttg aaagaaccat
    21721 tagaatcgac tgtattagta ttctttgcgc catatgaaaa gttggatgaa cggaaaaaag
    21781 taacgaaaca attgaaaaaa acagcaatta tcatcaatgt ccaacagttg aatgaaaaag
    21841 aagtacgtca atatctgatg aataccttag agaattcctc aatcaaaatg gaacgaagtg
    21901 caattgattt attcttgagg ttgaccgact tagatttatc caaaatgatg agagagttac
    21961 agaaattggt cctttatgga caaaatcaac agcaaatcac ggtaagagaa gtagaagaat
    22021 tagttcctaa aacgttagaa cataatattt ttgacatgac ccaatatatt ttaaaaggga
    22081 agacggaaca agctttgcgt ttatacgaag atttagttat gcaaggggaa gaaacaatca
    22141 aaattaatgc gattttgctt tctcaattac gtttattttt acagacaaaa tttcttgtaa
    22201 agattggtta tcagcaggca aatattgctg aaacattgaa aatccatcca tatcgtgtaa
    22261 aacttgccat gcaggaagta aaaaaattcg atgaacattt gctggtacag ttgtttgatc
    22321 aattagttga gatggattat caaatcaaaa cgggacaaat cgaaaaagaa ttaagttttc
    22381 aactattcgt tttgcgcaca gggcagttgt taagaaatcg ataagaagaa agacgagggc
    22441 aaagcatgcg ggaaggtcgt ttgtttttgt cctttttttg acaaataaaa aaatcggccg
    22501 aagccgattc ttttatttag cgattttttt gcttaaacga gatttgtcgc ggttagcttt
    22561 gtttttgtgg attagacctt ttgattcagc catatcaata gctttaacag cttctttata
    22621 taacgcatct acgttgtcag cacctgaagc tacagcatct tcaaattttt tgattgcagt
    22681 acgcatagcg cttgtttgag atgaattttt cacattagcg ttttcgcttg tacgaacacg
    22741 tttgattgca gattcaatgt ttggcattag tttcacctcc gataacttaa gttcttacgc
    22801 gtgttaccgc ataattcaac attcattatt atacctaaag gcagcgtgct ttgcaatatg
    22861 aacatgacaa gtttttttcc ttaacagtct tttattagga ttttttttca gtaaaaacgg
    22921 atagtacaag ctgaaaaaaa tcaaaaagac gctattgtgg gaggcaatag cgtcaaacca
    22981 aactagattt ttatttatct atttaaacaa agtttgaatg ggaatgtttg gtactaagtt
    23041 attatataac aaagagaacc gtttgcaaag ttttttttga aaagggatac gattccctgt
    23101 tgttctttgt ttttctatta agaaactgca gttattttat aaccagtatc ctttgatcag
    23161 taaaggctat tctggtgtac cgattatttt aatgtattca tcgatatttt ttatcaacat
    23221 tttttttatg accaactggt tccgacggtt ccatttgcca cttacttcca gcacatattt
    23281 atttaggggc aaacgtaaga tatattttgc caaatagcga tctttaagca ggccattgat
    23341 cacacggtcc tcaagctgca agttgatccc agtcagcatc attggctgtt cttgtaaaac
    23401 gtacgtatca attaaaagac cttcaaaagt ttccacaagc gtcttccttt cttttcaaag
    23461 taacctgaaa acaggcacta attcttccat tttgatattc gttgaagtgg atgcgatcat
    23521 tctttctttt gtccactgta atctagtata acaaaaaaga gcagaagagc caaaaagaaa
    23581 aaagagcatc gaattctata ggaagtctca ttggcacggc ctaaattatt acaaaacatt
    23641 gattaaaaaa agcccctgca tcgcaggagc atcccctata tcgttttaat aggtcaatca
    23701 aaaagcagta tcaaacacat tttgtatcaa atcaggaaat aatagataag ctaagcccag
    23761 aatcaaaagt accgctagaa gaaaccagcc gattgttcct atgaaatact ttaatttttc
    23821 gaacattgta tcacctcgtt gtcagtatat cacggatcag taaattgggg gtgttaagaa
    23881 tctcctaaaa tagacaaaat ttgttctatt ttcctaaagc aaatacttgt aagatacagt
    23941 aagtgatacc agaagcaatg accggtccgg cggcgattcc tcttaagaag acaacaccta
    24001 aaatcgtgcc gaaaacaagt gcaacagtga tttgtggatc agaagcaatc aatcctacac
    24061 ccttggaaga taagacagcc actagcccac cgcaaaaaac agctacccag ccaatagggg
    24121 atttaaaggc ttctaataga tcacgtagac cgatttctcc tgttgcgata gggattagaa
    24181 tagccgctgt gatgatcgtc accccaatat tgattccttt gccttgaagc caagggaaaa
    24241 aggaacggga ggctggtatg agcttcataa tcaaaacaaa agctgccgct atcatcagac
    24301 tttgattttt agctaaaatt gctacaagta aaataattcc taaaaaaagc caactttcca
    24361 tgaaatatat tcctcacttt cttcgaaaca atcataacat gtttttagaa agttacaaaa
    24421 gtaggaattc atccttcaag acctattcat aagctgtttt tttcataaaa taaacaaaat
    24481 cataaaaaaa cactttgctt atttgacaga agacagccgc gctttgtata gtggacaaga
    24541 cttacatttt tttgaagaat cagaaagatc aggtaaaggt cttagtaaac gcaagccatt
    24601 cagaataacc aatattgtgc ttccttcgtg tcctacaact ccaagaggta gatttaaaaa
    24661 ttgcaggaag ttagaagcaa tcaacaagag aataacaaga atcgaaaaaa taatattttg
    24721 tttgatgatc cgccgcattt ttaaagatag ccgatgactg tagacaaatt tatttaagtc
    24781 gcttttcatt aagacgatgt ctgcgatatc catagcggca tctgtccctt gccccatagc
    24841 gacacctaaa gaagcggctg ctaaagcagg cgcatcattg actccatcac caaccatcgc
    24901 atttacttgg taaagttcat tctctttttt gactagttgt gttttttctt ccggagtaca
    24961 gcttgcatag taatgttcaa tccctacttg ttcagcaatg gcctttgctg tgccagaatg
    25021 gtcgccggtt atcatacttg tatggatgtt ttgggattta aagtaggaaa ttacttgttg
    25081 ggtatttgct tttggaatat ctagcagacc taaaacagca attaactgct gatctttaga
    25141 aagataaatc acggttttgc cttgattttc caaacgttcg atcatttccg caatctcttt
    25201 gctgattatc atcgtctcag gatcatatgc gtgcttgcca actttccatt gcgcacctaa
    25261 ataggtagtt tgtaatccaa aaccagtaat ttcttctatt ggtaagtatt ggatttcttc
    25321 aggtattgtg caatcgaaat attgcaaaat cgcatgagca agtggatgcg tcgttttttg
    25381 ttccatagca acaagaagtt gttgtgccaa atccttttca tcaaagaaaa agctgtcagt
    25441 tacaactggt tttcctttag tgagcgttcc tgttttatca aaggcaatcg cttttaattc
    25501 agaaagcttt tctaaatgga taccgccttt gattaaaatc ccattttttg cagaatttga
    25561 aagagcagct agagaagctg gtgtagcaga tgcgacaagc gcacaaggag aagccacaac
    25621 taataatacc attcctcgat aaaagctttc atcccaactc caacctagga aaaagtgggg
    25681 gagaaggatc attagaggaa cagcaagcaa aatcaatttt acataattat tttcgattcg
    25741 ttcgataaat gaagctgttt gagttggcgt attctgcgct tcttctacta attggataat
    25801 cttagcaaag acagtttcgg aactcgtttt tgttactcga atcgtgatgg gatttccttg
    25861 attcagcgtt cctgcataca tctcatcgcc taaatatttt tctacaggca cagattcacc
    25921 gcttaatgca gactcatcga tcgttgcctg tgtgctttct aaaaagccat ctatcggaac
    25981 agctgctcct ttagcgacaa ataccaagtt atccaccttt agttgagaaa catctacttc
    26041 ttgcatttgt ccattttctt ttaataattg ggctttttgt ggttgaagat tcatcaaagc
    26101 agtaatttct ttttgacttt tattagttgt gtattcctct aatgcaccgc ttaaacaaaa
    26161 gatgaatgtc aacattgctc cttcaaagta attaccgatc aaacaggcac caatagcagc
    26221 taaagccatc aataaatcca cattcagtgt ccgatcattc cacaattctt tcaagccttc
    26281 cgatgtttgt ttatatccac cgatgataat ggcactgata taaaaaagtg gggtgatggt
    26341 aagagagaat cggtcgaaga taaaaccgaa gatcataaat aaaaggcagc cgattgttgc
    26401 tgcaaaagct tttttctgat tagtcatcgt tattagacct ccttgtcaaa acaaggatag
    26461 cataataaaa atgtaattga taataataac agctaaatga gaacgataat ggttatcatt
    26521 tggtgattaa gcttgatgtt gtttttatta tttataatga ttattattaa agaaaataaa
    26581 aaaccagaac atttctcaaa taagagaagt gttctggttt tttctacatt gcaataaacc
    26641 aataaccaat caaaatgata ccaaggataa tacgatacca gccaaaagcg gtaaagtcgt
    26701 ttttcttcaa atagcttagt aagaatttga tagctaagac ggaaaccaca aaagatacga
    26761 atgttcctac aagaagaaca acagtttctt ccattttgaa tgaattccct tcagctaaat
    26821 atttgacgat cttcaagccg cttgcgccaa acatgacagg aatccctaag aaaaatgaaa
    26881 actctgtagc gacaaaacgc gaagcaccaa tcaagataga tcctaggatc gttgcgccag
    26941 aacgggatgt tccgggaaca agtgccaaga cttggaataa cccaacaaga agagctgctt
    27001 ggtacgtaaa gtcattcaga gaagtccaac gaggtgtttt gtttttgttg cgtttttcga
    27061 caataataaa ggcaataccg taaacgatca aaacaatggc aactggtaag aacttgtgga
    27121 aatttgcttc taaccaatca tccaaaggca aaccaatgat gatggctggt atgcaagcaa
    27181 cgatcacttt aaaccaaagt gtccacgtat cttttttttc taccgttgat ttacttggag
    27241 aaaatgggtt taacttgtga aaataaagaa cgacgacagc caagatcgcc cctaattgga
    27301 taacgacatt gaacatcgac ataaattgtg aactggcatc cagtttgatg aattcatctg
    27361 ctaggatcaa atgaccagta ctactgatgg gcagccattc cgttattcct tcaataatac
    27421 cgagaatcac tgctttgata atatttgcaa aaaacataaa ttccttcctt cccaattaag
    27481 atacatctta tagtataccg ttattttttg agaaacccaa tctgtttttt aatattaaga
    27541 aaaacttgag aaataaagca gataagtaca cgaagagaaa aaagaagaca aagccctcgt
    27601 tcagactttg ccttttatga ttctctggta aaaattaaaa tatccccatt atagaaacga
    27661 gtcgcgacta tgtgttttcc cgttatcgat ataagacgta tcattgatgc cgtggataga
    27721 aaatcgaccg tttgcataag tgacctttgt tacgctgcca tttaaaagcc cagcacgaac
    27781 aggctgttta ggatcgatca gattaagcaa aaaggcgatc gtcaaaccat gagatacgac
    27841 taaaacattt ccaccaccat ttttttcgca gtgatgggcg atatcttcaa atcctgtcca
    27901 tacacggtct cgcaatgttt cataagtttc cgcccaccca gcggtatcta agttgtatac
    27961 agcattagca atttgttcaa aagaaacttc tgtggtaaac atttcatcat atgttttaaa
    28021 attcaataca cgagggatca caccccacat ttccatgtca tatccacctt caagagaccc
    28081 gaaacaccat tctctaatcc ttttttcaat aaaataagga atttcttttc ccttagaatg
    28141 ttctcctaga ataatacggg cagtttcgat tgcacgacca ctgtcactag aataagcaaa
    28201 tgaaaagtcg acatttttta acccgcgacc taaataatga atgccttctt gtccttgctt
    28261 tgtgaggggt gtatcacacc agccttgtac tcgttcaatt gtattgaaca tcgtttttcc
    28321 gtgtcgtacg atataaagtg tagtttctgt cattttattc tcctcctaag tcgttttaac
    28381 gattgaaaaa aggatttgtc cttttttcct gttcaatcgt cgttgcatcg ccatgtcctg
    28441 gatagactgc aaattcatca ggtaatacaa acagctgggt ccgaatacta tgaagtaatt
    28501 gttctaaatt ccctgtatac agatcggttc taccgatact tcctctaaac agggcgtcgc
    28561 cagaaacgac aaaatcatca aaaataaagc tgacgcttcc tatagaatgc cctggggtag
    28621 ggacgacgcg aaaagacatg tttcctaaag tataagtttt catttcaaat tcatattctg
    28681 caggcgagac aataacatca gcaatatcat catgtctacc gagtccagaa agattcaaaa
    28741 tcggatcacc tagccatttt tgctctagtg ggctgacata aactgggatt tgataatacg
    28801 cgcgtaaagg ttccactgca ccgatatgat cgtagtgagt atgggtaagc aaaatcgcta
    28861 ccggctgttg gtttgttttt ttgatttctt gtttgattcg ttccgcatct tctccagggt
    28921 caatgatcaa aagtgcttct tcgttgtaga ccaaataaca gttttcttca atcattcctg
    28981 tttttaatct ttcgatatgc atgtgcattc ctccttgata gccaaaactt ctttcatcca
    29041 ttagtataac gcattcattg ctaggaaaca aaaacaggta cttatttctc tttacttatc
    29101 tgaattatca gactatatga aaagtatttg gatattctaa agagaaggaa agtaagtttt
    29161 catagcattt taatatgtcc ttgttacatt agattcatcc aaaaatccaa attctatatc
    29221 ctatatccct gcctaatcag cagggtgttt ttttatgttt ggaaagagag ctctttttgc
    29281 taaactaggt tttttcaggt aatcttagag agaaagaggt ggtggaaatg atcaaacagt
    29341 caaaaatatc tgtgcggcac ttagtagaat ttattttgcg acgtggaagt atcgacaatc
    29401 gtaaaaaaag taaccatact gcattagaag gagcgagaat ccaccgcaag ctccaaaaag
    29461 atgcgggaga aaactatgaa aaagaagtct ttttaaaaac aacagttcaa ttagatgaat
    29521 attacctgat cgttgaaggc cgtgcagacg gtattttcaa aagagacggt atttattata
    29581 tagatgaaat caaaacctcc gaaccacgat ttgaagattt agaaccagaa caagtggaac
    29641 ttttctttca tcaagcaagg gtgtatgcct atatctatag tcaagaacat gatttacaag
    29701 aaataaatct gcaattgact tacttccaga cggcagaaga agtgatcacg agaaaggttg
    29761 aacatcagac aagagagcag ttagaaactt tttttaaaaa gctgactgaa gattataaag
    29821 aatggcttgt ttttcaagaa aactggcgca ctgtacgcaa tgcctctttg atggctttga
    29881 aattccccca tgatgagtat aggaaaggtc agcgtgaact agcggtagct gcttataaaa
    29941 cgatccgtac gaaacagaaa ttgtttgtag aagcacctac cggaacagga aaaacaatct
    30001 ctacgttatt ccccgctttg aaagctgtag gggaagaaga gggagaaaag atcttttatc
    30061 taacggctaa aacaataact agacaagtag ctgaagatgc tatgacagca ttaaaagata
    30121 caggagctga agtaaaaagt gtcactttga cggcgaaaga caagatctgc tttttagatg
    30181 agacgacctg taatccagat cagtgcccat atgccaatgg ttattacaat cggattaatg
    30241 aaggtttatg ggatcttttg aatcatgaga atcagatcac tcgtgaagtc atcgaaactt
    30301 atgcaagaaa gcatacactt tgtcctttcg aactatcatt agatgtcagt atctggtgtg
    30361 acgtaatcat tggggattac aattatttat ttgatccaac tgtttattta agacgttttt
    30421 ttgaagatga aaaaaacgag gactatctgt ttttgattga cgaagcacat aatttggtga
    30481 atcgttcccg agagatgtat tcggcagaac tatcttatga aaaaacgaaa cgaagtaagg
    30541 aagctatacc aaaggaattc aaaaaacttc atcgccgttt caacaaacta ttaaaagaat
    30601 ttgattcgat ccgagaaata gcaaaagaag atcattggga ttatcatcat caaaaagcac
    30661 cggctgaatc tttagtaaag gcaggttatc aattaagcga aaaaattaaa gaatggctgg
    30721 cagaatttcc tgagcaccca tcacaagaac aattgcttcc ttattatttt gatttgcttc
    30781 attttttgaa ggtcagtgaa ttttatgatg atcactacga aacaacagtc gaaaaaacgt
    30841 atcgcgatct gatagtaaaa gagttttgta ttgatccaag cttgtttctt gagcagtcgt
    30901 tggataaagg aaaaagcagt attctttttt ctgctagttt ttcaccactc tcttattatc
    30961 aggaaacact ggggggacaa aaaagtctgg cttacaaact gccgagtcct ttcccagaag
    31021 gaaaccagca agttttcatc gcaaattacc tagagacgac ttatcgaaaa agggaacaca
    31081 gtctggcaaa attagtagag acgattcatg cttttgctga tggaaaggtc gggaattatt
    31141 tcgttttctt tccttcttat caatatttag atcttgcggt ggaagcattc agacagcaat
    31201 atcccgacag caatgtgcta atccaagaaa cggacatgaa tgaggaagaa cgcgaacgat
    31261 ttttaacaaa atttcaaaca aatcccgctg aaacattgat tggattttgc gtacttggcg
    31321 ggattttttc agaaggaatc gatttgagag gggaccgatt gatcggcagt atgatcgtag
    31381 gtgtaggttt gccgcagatg aatcatgagc aggaactgat caaatcatat tatgatgaga
    31441 aggaacacct tggatttgct tacgcttatc agcttcctgg aatgaacaaa gtcttgcaag
    31501 ctggtggtcg agtgattcgt ggcatggcgg atcaaggaat cgttgtattg gcagatcaac
    31561 gattttctag tttttcttat cgacgtttat tcccacaaca ttggcaaaca gcaagagaag
    31621 tacgaagtgt tggggaattg gaagaaggaa tacagcgttt ttggttatca aaagaagcag
    31681 taaatcaaaa aaacgaggaa tgacagacgt cagcctcgtt ttttgattaa cgatagttag
    31741 tgaattgcaa ctcgattggt aagtcaagtt tacgtaataa agcaatcaca gcttgtagat
    31801 catcacggtt cttacctgtc actctaatct gatcttcttg gatttgtgct tttactttca
    31861 gtttttcatt tttgatagct gtagtgattt tcttcgcgtt ttcacgatcg attccgttga
    31921 ccaaatcggc agtttgtcga gctttgccac ctagagcgtg ttctgattcg gaaaaatgga
    31981 tatttttgat tggcacattt cttttgatca gcttaccaaa taatacatct ttgatctgtt
    32041 cgattttgaa atcatcatcg ctcaatacaa ctagattgct gttctctaat ttgatttcta
    32101 ctgtagaatc cttgaagtca aagcgattgg acaattcttt tgttgtttgc tgaatcgcat
    32161 tttttacttc ttctgtattg acttctgata caatgtcgaa gctagcatct tttgccatag
    32221 gaaccctcct gatatacata taaatattta ccatacatct atcttatcat aggattaggg
    32281 cagattctta cctgtttgct aaaattcttc tcatttgagt tcgaagtatt ttattttaat
    32341 agataaactg ttattcttac atttggaata ccaaagtagg ggagaggtgc ttgtgagtaa
    32401 aattatgact gcgtatcata aaaatgaaag attaaagaaa atagtggtta ttttgataac
    32461 cggattgacc gctgcggtcg ctttaaatta ttttttgatt ccagccaatg tcttttctgc
    32521 agggatgaat gggatcgctc aaatcatttc ctcgctttta tatgaatggt ttcatatcaa
    32581 gatagacaca ggaacattta tttttctttt gaatatccct gtgtttgtat tagggtttct
    32641 caaagtagga aaacgagcaa cgatccttag ttttgtgaat gttgtatgtg tatcggtgat
    32701 gacgatgctg cttccagtcg gacagatcac tgataatatt ttgatgaatg ccctagtagg
    32761 cggtgtttta ttaggtcttg gcgtaggatt atctttaaaa atgggcttta caactggtgg
    32821 gatggatatc atctctcttg tactttcaca aacaactgga aaaacagtcg gaaactacat
    32881 gctgattcta aatggtatca ttgtagtcat cgcaggcttc ttctttaact gggaaagtgc
    32941 cttgtatacg attatttcca tttatgcaat gactcaagta attgatgcca tccacacgag
    33001 tcatcaaaaa gtaacagcaa tgatcgttac cgtcaagcca gaagaggtaa cagaagaact
    33061 agcaaaacgg atggtacgtg gaatgacatt actgccgtct gtcggtggct attcaaaaaa
    33121 acaagggaac atgatcatga tggtcatcac ccgctacgaa atgtatgatc ttgatcagat
    33181 cgttcatgaa atcgacgacg atgcatttat caatatttta ccaactcatt cagtatttgg
    33241 ccggtttgca aatgaaaacg aacaaaaaat gtatcgaaca actggcgtat tgccagaaat
    33301 aaaaaatcgt tcacaaaaaa aatgaggaat caggtttatt ttagaataaa tttaatctat
    33361 tacatctaat aagaacattt ttataaattt aaacgagaat cttacttcta aattctcatt
    33421 tacaagcagc taaaaaaaga gtatgataaa taaaaatcgt gttaaggagt gtcgtacgat
    33481 gagcgaagca aaccaagaac ggtctggaca agaaatagaa ctaatcaaag aacgtattct
    33541 tgctgccttg gaaatggtga ttgatcctga attaggaatc gacatcgtca atttaggctt
    33601 gatttatgat atagaattca atcctgaaaa tggagaaaca gtcattaaga tgactctaac
    33661 aactatgggc tgtccattag cagatatttt aacagaatct atccatgacg cattaaaaga
    33721 agtgccagaa gtaacgaaag cagaagtcaa actagtctgg tatccagcat ggacgacaga
    33781 taagatgagc cgttatgcac ggattgcgtt ggggatacga tagaaatctt ggctgactag
    33841 ctatgagaag caaataggaa aaacaaaaaa gccttttatc ctttctagaa gaaaagagat
    33901 aaaaggtttc tttgtttata aaattttgtc tgctatatta taagttaatt aaataaatgt
    33961 gttctattga taaaggacat tcaaatccct ttccttttgg aatccttctt ttttacatca
    34021 gtttgatttt cgtatagtat tctttctcat cttcacgata aatcgcagcc ccattttttt
    34081 gttgtgcttt aagactgcca agattttttt tatttacaga caaaagtgcc tcatcaatgc
    34141 cgattttttt tgcctcttgt agtactaatg ctaatccttg tgtggcatat ccttttttac
    34201 gataattagg ggagatgccg taaccaatat ggccagcgcc gtttcttaat gtttcgttca
    34261 gtttatggcg caaattaaaa attcctacat actcatgttc atcactttct aaaataaaga
    34321 cggttgcggg aacgtaactc tcaggaagat cgattcctaa agacgttttt tcgcattttt
    34381 ttacataatc taaaaattca gcaaatgtat agccataagc attgttgata aatccactct
    34441 catttggtcc aaagctagtt tgtagctgat aagctttctt taaatctgct tcccatagtt
    34501 tgatcagttt catcgtattc acctctttaa gtaaccttat tcatttgttt tctactatag
    34561 cactattgtt gtaaatagcc atgcctattt ttatttattg tgtcatatcg tattaagaat
    34621 catcgaacaa tacaaatatg aattacaata aaaaaattat ttgaagttcg ttcattcgtt
    34681 acttatgatc tctagaagaa agaaaacgtg aattacttca gcgtctagaa ttccagaagg
    34741 atagtaatca acagaagaaa taatcaatat ccaaatgaaa gaagatagct gaatcgagaa
    34801 aaagaggaaa aaagaaatga agaaaaaaat aaaatagtta ctaatcccag agtcgttcgt
    34861 ttaacattga aatcgatcat atggataaaa cagatttttt caccaaccct caatagatga
    34921 tggagttgct cggatgaatt atttgtgtag atggcataag ggagggcgta cagaaatgaa
    34981 aaagaacaaa aatatgttct tgacaaaaaa cacatgtaat cctaaactaa aagatgtgat
    35041 gcggctatgg cggaattggc agacgcgcta gcttcaggcg ctagtgaggg tgacctcgtg
    35101 gaagttcgag tcttcttagc cgcataattt gtttttttat aaagagaagg tacacctgga
    35161 gcgatgagaa agaaaataac atattatcta aaagaatata ggaaaaattt taaacaacgg
    35221 aaccagattc aaataaatca aaaaaaattt gtgtgaaggt gggacagttt aaaacctaat
    35281 agattgattc aaatagtaga tttatagatt ctgatacaag aaatactggc aaacgatagt
    35341 taaggaataa agactaaact taaactcaaa aagaggtcat gcgaaagaat aaatcgcatg
    35401 acctcttact atattttgaa tgtgtttaaa cgttcaaa
//
LOCUS       DACEUZ010000012        34668 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.012, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000012 DACEUZ010000000
VERSION     DACEUZ010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34668
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.012"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(311..2203)
                     /locus_tag="IXG44_000520"
     CDS             complement(311..2203)
                     /locus_tag="IXG44_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1657259.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(2200..3927)
                     /locus_tag="IXG44_000521"
     CDS             complement(2200..3927)
                     /locus_tag="IXG44_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1657260.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(3943..4593)
                     /locus_tag="IXG44_000522"
     CDS             complement(3943..4593)
                     /locus_tag="IXG44_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1657261.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            4797..5093
                     /locus_tag="IXG44_000523"
     CDS             4797..5093
                     /locus_tag="IXG44_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAQ1657262.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            5285..5905
                     /locus_tag="IXG44_000524"
     CDS             5285..5905
                     /locus_tag="IXG44_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HAQ1657263.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(5997..7163)
                     /locus_tag="IXG44_000525"
     CDS             complement(5997..7163)
                     /locus_tag="IXG44_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ1657264.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(7175..7528)
                     /locus_tag="IXG44_000526"
     CDS             complement(7175..7528)
                     /locus_tag="IXG44_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAQ1657265.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLKTVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            7656..8678
                     /locus_tag="IXG44_000527"
     CDS             7656..8678
                     /locus_tag="IXG44_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HAQ1657266.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            complement(9117..9551)
                     /locus_tag="IXG44_000528"
     CDS             complement(9117..9551)
                     /locus_tag="IXG44_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1657267.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLMKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(9661..11520)
                     /locus_tag="IXG44_000529"
     CDS             complement(9661..11520)
                     /locus_tag="IXG44_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAQ1657268.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(11638..12798)
                     /locus_tag="IXG44_000530"
     CDS             complement(11638..12798)
                     /locus_tag="IXG44_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HAQ1657269.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(13105..14529)
                     /gene="araA"
                     /locus_tag="IXG44_000531"
     CDS             complement(13105..14529)
                     /gene="araA"
                     /locus_tag="IXG44_000531"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HAQ1657270.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(14545..15243)
                     /locus_tag="IXG44_000532"
     CDS             complement(14545..15243)
                     /locus_tag="IXG44_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAQ1657271.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(15200..16861)
                     /locus_tag="IXG44_000533"
     CDS             complement(15200..16861)
                     /locus_tag="IXG44_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HAQ1657272.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKG
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            17079..18479
                     /locus_tag="IXG44_000534"
     CDS             17079..18479
                     /locus_tag="IXG44_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HAQ1657273.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKGLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIVGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(18731..19810)
                     /locus_tag="IXG44_000535"
     CDS             complement(18731..19810)
                     /locus_tag="IXG44_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1657274.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(19896..20855)
                     /locus_tag="IXG44_000536"
     CDS             complement(19896..20855)
                     /locus_tag="IXG44_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HAQ1657275.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(20867..22390)
                     /locus_tag="IXG44_000537"
     CDS             complement(20867..22390)
                     /locus_tag="IXG44_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAQ1657276.1"
                     /translation="MDEEVKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHE
                     TADGDGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIE
                     PNSFGLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQ
                     KKPFGVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPR
                     LPSYPEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFD
                     RQIEEIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMED
                     TLLFGSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAK
                     GQVLKQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLL
                     DQGEHYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(22400..22963)
                     /locus_tag="IXG44_000538"
     CDS             complement(22400..22963)
                     /locus_tag="IXG44_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAQ1657277.1"
                     /translation="MKIVETKLYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(22969..23832)
                     /locus_tag="IXG44_000539"
     CDS             complement(22969..23832)
                     /locus_tag="IXG44_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1657278.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(23846..24742)
                     /locus_tag="IXG44_000540"
     CDS             complement(23846..24742)
                     /locus_tag="IXG44_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1657279.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(24917..26188)
                     /locus_tag="IXG44_000541"
     CDS             complement(24917..26188)
                     /locus_tag="IXG44_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1657280.1"
                     /translation="MKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTLTFWNGF
                     TASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFIALNYPD
                     FAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMYMYWNND
                     LFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLANGGKLV
                     NDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIEINGPWL
                     NNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFLKYWNTT
                     EIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQISAINNDI
                     INPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(26178..27695)
                     /locus_tag="IXG44_000542"
     CDS             complement(26178..27695)
                     /locus_tag="IXG44_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAQ1657281.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            27893..28783
                     /locus_tag="IXG44_000543"
     CDS             27893..28783
                     /locus_tag="IXG44_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HAQ1657282.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(28856..29680)
                     /gene="nadE"
                     /locus_tag="IXG44_000544"
     CDS             complement(28856..29680)
                     /gene="nadE"
                     /locus_tag="IXG44_000544"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HAQ1657283.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(29692..31164)
                     /locus_tag="IXG44_000545"
     CDS             complement(29692..31164)
                     /locus_tag="IXG44_000545"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HAQ1657284.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(31801..32163)
                     /locus_tag="IXG44_000546"
     CDS             complement(31801..32163)
                     /locus_tag="IXG44_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAQ1657285.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            32386..33138
                     /locus_tag="IXG44_000547"
     CDS             32386..33138
                     /locus_tag="IXG44_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ1657286.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(33278..34201)
                     /locus_tag="IXG44_000548"
     CDS             complement(33278..34201)
                     /locus_tag="IXG44_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ1657287.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(34216..34488)
                     /locus_tag="IXG44_000549"
     CDS             complement(34216..34488)
                     /locus_tag="IXG44_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1657288.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT        10232 a         7212 c         5981 g        11243 t
ORIGIN      
        1 agaggttgtg agcctgccgt ttaactccga gtcacaagga gcaatttcaa aaaacagctt
       61 ctcaggtttg ttgactattg tgacttgtga ccgaggagtt ggcaggagaa caccgtttaa
      121 ggaagaggtt gtaaaatcag cggtttgacc tgagtattag agatacaaaa cggaaaaata
      181 gcttctcata ttttgccgtt ttttgagcta ataccgaagg acagctgatt gaacaccgtt
      241 tattcggaaa aagggactgt gacacgactt ttgtcacagc ccctaaaaaa aataataaat
      301 ttgtactatt ctatgaagct tcttcttcag aaaactgact gttataaaga tctgcataga
      361 atccgtcttt ttccatcaat gtatcgtggt ttcctgtttc tacgatcgaa ccatgattca
      421 tcacaataat gttgtctgca tcgcgtattg tagacaagcg gtgagcaaca acgaaacttg
      481 tccggttttc caataggcgg ttcatcgctt tttgaatcag tatttctgtt cgtgtatcta
      541 cactagatgt tgcttcatca aggatcagga catctggatt tgctaagaat gcccgcgcaa
      601 ttgtgattag ctgacgctgc ccttgcgaaa tattgcttgc atcctcgttt aatacagtgt
      661 tatatccttc tggcaattta cgaacaaaat cgtctacgtg agcagcttta gcagcctcaa
      721 tgatttgttc gtctgttgca tcgtcgttcc catatttgat attgtcatag atacttcctg
      781 taaacagcca agtatcttgc agtaccattg aaaactgtgc gcgcaattca tcacgtgaaa
      841 gatcacgggt atctacacca tcgtaacgaa tactacctcc agaaacatcg tagaaccgtt
      901 caagaaggtt gatcagtgtc gtcttccctg caccagtcgg acctacaata gcgaccatct
      961 caccaggttt gacatccaag ttaaaatcag tcattagcaa gttatctggt gtgtagccga
     1021 attgaacatt ttcaaaacga actttgtatg gtgtatcttt ttcttctgga aggccagatt
     1081 tttcattgga catctcttct tcatccaaca cttcaaatac ccgttcggct gaagcaactg
     1141 ttgattggat agtgttcatc aaacttgcaa gttgagtgat tggttgagag aactgtgttg
     1201 tatattgcaa gaacgcttga acatccccta atgtcatgtt cccatttgca acttttactc
     1261 cgcctagtac agcgacgaac acatagccta gatttttaac gaaattcatc aacggcatga
     1321 tgattgccga aataaattga gctttccaac cagcggcata cagattctga ttttcttttt
     1381 caaatacttc ctggtctttt tcagtatgat tgaatgtttt aacaatggta tgaccaccat
     1441 atgtttcttc tacttggtta ttcaataaac ctaaactttt ttgctgatct gcgaaaaaca
     1501 cttgtgattt cggcgctacg atcatcacga tgatcaagct taaagggatc atcaataacg
     1561 cgactaacgt caattgccag ctgattgaaa gcatcatcac gatgacccca atcaatgtga
     1621 ctgcactagt tacaaattga gtcaaatttt gctgcagcgt gcttgcgatg ttatccatat
     1681 cattgattgc tcgagacatg atatccccat ttgtatgtgt atcataatag gaaatcggta
     1741 cacgattcat tttggcttcc aaatcatgac gtaattcata aactgttcgt tgggaaacac
     1801 gtgtcataat aaattgctgt aaaaagttga atacagcaga aatcaaatac atcacgatta
     1861 ctgtcgcaat gatctgcgcg attttatcaa agtctatcgg aaattttgtt acttgttggc
     1921 cttgttgcat ctgttgagcg ccagccatta cacctttgaa tatttctgtg gttgctttcc
     1981 ccaagacctt tggtgtttgg atttggaaga tcacggctgc tattgctagt acaaatacag
     2041 cgatgatcgc aaccatacgt ttagacatat agccaaacaa tcggcgtacc gttccccaga
     2101 aatttttagg cttttcacct tttacaccga gatttctgcc aggtccacca tgtcctggac
     2161 cgcctgctgg tccatgagag ggacgtgtgt ttgttttact catgctaagt cctcctctct
     2221 tagttgtgaa tgaacgattt cttgataagt ttcgtttgtt gctaatagtt cttcatgtgt
     2281 tcctttgcca accagttttc cttcatctag cacaaggatc atatctgcat ccattactgt
     2341 acttactcgt tgagcaacta aaacggtgat actttctttc atattcgttt tcaatgcttg
     2401 gcgaagattg gcatccgttt tgaaatccaa cgctgagaag gaatcatcaa acacatagat
     2461 atctgctgtt ttgactaatg cccgtgcaat tgccagacgc tgtctttgtc caccagagaa
     2521 gtttcctcct ccttgttcca catggctatc taagccatct tccatctctg aaacaaaatc
     2581 tttagcttgt gcgatttcta atgctttcca aatcatttca tctgttgcat ccggcttacc
     2641 atactgcata ttgtctcgga tcgttccaga gaacagaact gctttttgag gaacaaagcc
     2701 cattaattct cgcaaattat attgagtcat gtcactaatg tccgttccat ttatttggat
     2761 cgaaccagat tcgatgtcat ataatcgagg gatcaaattg actaaggtcg ttttacctga
     2821 accagtcccc cctatgattg cgacgatctc gcccgacttg gcttggaaat caatatcttc
     2881 caatgcaagt ttttctgctc catgatatcg ataattgaca tgattgaagg ccaatgttgc
     2941 gttttttcca gcaaaagaaa ctgttttagg attttcaggg tcatggatac cgattttttc
     3001 atccaacact tcattaatac ggtcagctga agcttgtgca cgaggcacca tgataaaaat
     3061 agcggaaagc atcataaagc tgatcaagat ctgcatcgca taagtcataa atgcaatcag
     3121 attaccgact tccaaagtca tatctgctat gtagtgtcca ccaaaccaag tgattgctac
     3181 gtttgtaccg ctcatgatca atgtcatcat tggcatcatc aatgcgacga tcgtattaac
     3241 tttgattgcc gtatttgtat aatctttatt cgctaaatca aaacgatttt cttcaaagcg
     3301 tgtcttatcg aatgctcgaa tcactctgac ccctgtcaat ccttctcgaa ataccaggtt
     3361 taaacggtca gttttctttt gcatactttt gaataatgga acagcaaagt acatgattcc
     3421 gccgatcaaa ataatcatta ctgggatgac aactaagaag atttttgtca actgatgatc
     3481 tttttgataa gcaagtatgc tcgcgccaat caacgtgatt ggtgcattga tcatcatacg
     3541 taaaaacatt tgtgtaacca tttggatttg agtcacatca ttggtcgttc ttgtgatcaa
     3601 agaggccgtt ccatatttat caaacgcatc ttttgtcaga ctctctgatt ttttatagac
     3661 atccgtacgc aattgcttac ccagtttctg tgattcacga gtagcaaaaa atgtatttgc
     3721 gatagcagct aagatactga tcaaagaaaa gccgatcatc acaaaacctg tatgccaaat
     3781 ataatcgaca tctccttgcg ctaccccttt atcaataata ttggaggtaa gtgtcggcaa
     3841 atataaatct gccagtactt ggatcaccat gaaaactgca gctgcaattg ctgaagtcaa
     3901 ggagatccgt tttacgattt ttagcatcta gttcccttct ttctatttat atactccgtt
     3961 tttcagccaa gaaagtttca aattgaattc actgattgct tgctctactg agtaatcttc
     4021 agatgttgaa aataagcgat acgaatgcgc aattgttgta aatacagtat tcatcactaa
     4081 ttggatcagt aattccaatt catgatcatt ttttactttc agcttcgtcc gatcgatcaa
     4141 ttcaagcaac tcttttgcat cttgtttgtg ttctttatgt ggatcgtgag gcgttttttg
     4201 gcgactcgta tgaggagcga cttttcttga ggcacggtaa tccatgttca taaacaagtt
     4261 acgataaaaa gaagcattct cgccttccaa aatctcataa atcattttag aaaaatagga
     4321 ttcgaatcct ttgaatagat caccatcgga ttctttcatt agttgtaaca tatctttaga
     4381 gctgtcacgt ctcattgtct caaagtaata aaaataaaga tcctctttat cttcaaaata
     4441 ctgatagaaa ctgcctctag ggatatccgc taatttgacg atattagcaa tcgaagcttc
     4501 ttttaagggt actctagaaa actcgattct cgcagcctcg attagtcttt tttgtttttc
     4561 tttaggcaaa tggaaaaatg tctgtgtcgg catatggatt ctcctttctt tcaagatgac
     4621 acattgtcat atgacgctat gtcattataa cacctcctac ctgttttgca agacatttgc
     4681 atagtttttt tcattatcat ccgatacatt aaaaaagtga taagaggaat ccttccattt
     4741 gaattaatga gagttgccca ttaaaatagc cacatactaa acaaaggatg agataaatgg
     4801 cccaatcaat tagtgtccaa gacttcaata aattgcctct cgatgctgac agcatcgttt
     4861 tagatacaag aaatacctta gattacacaa aaaaccactt aagccactcg ctttctatcc
     4921 ccgctgctat cttgccaaga caattaaaaa atctagatcc aaagaaactt tattatattc
     4981 taagctatac aggcaggcga tcagaagcat tagccgatca acttactgct aatggttttc
     5041 gtgctgttta tatcattggt gggatggaag ctcttcagtc gagtgttgct tgaatattgc
     5101 ttgttagatt caaaagaggc tgtgacaaaa aatcgtgtca cagcctcttt tataccctgt
     5161 ctttttttag ttatttattc ttcaacctta tcaatatgac ttgatagcct tttctttttc
     5221 gagtaaactg attttgttag tcatagttat aacaatcata gcaataaaag gagaaggtaa
     5281 gcatatggca tctgttttag tagtcaaagg tcatccccta actgcagaag aatcgcgcac
     5341 agttaaggca ttgacgtctt ttttaacaag ttataaagaa aatcatccag atgatgaagt
     5401 gactgttttg gaactttatc gcgatgatat tccggaaatc gatgaagaat tattatcagg
     5461 ttgggaagca ttacgtgctg gtgcagaatt cacttcctta tcagaaagtc aacaacaaaa
     5521 aattgctcgt ttcaatgaat tgactgatca gttccttgct gctgataaaa tcgttatcgc
     5581 aaacgcctta tggaatctaa atatcccaac aaaattaaaa gcttggttcg ataccgtcaa
     5641 tgtagctgga aaaacgttcc gctatacaga aaacgggcca gaaggattag tgacaggtaa
     5701 aaaagcactg cacatccaat ctaatggcgg tgtatacaac ggacaagact ttgcctctca
     5761 atatgtaaaa ggtattttaa actttgtagg catcgatcaa gtagatcagc tattcatcga
     5821 aggaatcgat tatgaccctg accgtgcaga tgagttgatg caaaatgcat tagataaagc
     5881 tgctgcttta ggtaaatcat tttaaaaatt gagataaatg catacaaaag agacagagga
     5941 aaaaatcttc aaagcagcat ttgtgccttg gatattttcc catctgtctc tttttgttat
     6001 tctttctgct ttttcaacgg attttcttca taaagagaag agattgttgt gtaccattca
     6061 aaaacatgag taatcactac tttagctgct gcataaacag gaatccccaa gatcacaccg
     6121 atgattccga acaatttccc agaagtcagc agaactaata aaatcgttac tggatggatt
     6181 gctaactgat tccctagtac taatggagag atcaagcgtc cttctattgt ctgctctact
     6241 gcaaaaacga tcagtacttt gatgatcatg aatggacctg ctacaatggc taaaaagata
     6301 gccggaatca tcgctaaaaa tgatcctaag taagggatca ggtttaaaaa acccgcaata
     6361 atccccaacg tgacagcata ctctaaacca atgaccgcaa aaccaatcat aaacatgatt
     6421 gccacagcaa aagccacagt tagttgtcct cttatataag aagaaacttg actattcatt
     6481 tcactcagca cattcaacgt cggtttttgc atgcgtgtcg gtaaaaattt gacaaaataa
     6541 ggtgctaatt gtttaccgtc tttcaataga taaaataaga taaacggcat cgtcagcaga
     6601 gccactaaaa tagtcgctac cgcgccgaca aaacttccaa gactctgaac tgtactctta
     6661 gatatatctt ggatcatatc gcctgccgaa ttcatgaact tatcacctgc tgtttccaac
     6721 tgttcgcgaa attggttgaa cagcgggtcc cgcaaaatct cggttaattt attatcaatc
     6781 gtatctacat attgagggaa gttactgatg aagctcactg tttgttcctg aatcttaggg
     6841 atgatcacta cgctccccca tgcgattagt gcaacaacca aaacaaacag cccaatgata
     6901 ctgtaaagcc ttggtacttt tcgcttttca aagtaatcaa ctaccggatt catcaagtaa
     6961 tacaagattc ctgctaaaat gataggcaga cctacgattg ctaaaaattg ccagattggt
     7021 gcaaacaaat atgaaacttt tgtaaataaa aagacgatca gcaatattaa caatacaaca
     7081 agtaaggctg tgactacttg attatttaaa aaccagcgcc aaaaccacga taccggtctg
     7141 ttcttcttat tttttccttc cataattcct cctgttagtt ataaaaaatt tggcttccca
     7201 aaccaatagc aggcagtcta tgcggatgaa gatagattcc atttgcaatt tgtccttctt
     7261 ctggacaagc atcaaaaatc aatgatacgt gagctactgt ctttaaattc ccattggcca
     7321 tcggaccgac ataatcgatc gtatatacct gatcatcaaa agcaattgtt ccgcctgctt
     7381 tcaaagaaaa aggttcgtct tttgatactt cttgaatcac cgaatatttt cgtaaggcag
     7441 ctgttgctgt tttatcgaac aaaatcagca tgggttcttc cttgtttatt gcttctgtcc
     7501 caatttctgt aatctttgcc tgaatcattg cactctctcc tttaattgct ttcaaaaaca
     7561 gtataacaca ttctcattct ttagccgtgt ggaataatta tgtgatagaa taaaaacgaa
     7621 aactatttca ttacaattag gaggaatcag ctcatatgat cgaaaagata attttaggaa
     7681 cctatacgcg tcgcgaaagt aaaggaatct atacgatcga ccttgataca gaaaaagaag
     7741 aattagccaa cctttcatta gctgttgaag aaaatagccc tacctatgta gcaaaaagta
     7801 aagctggcaa tctttacaca gttactagtg tggatggatt aggtggtgcc ggtgcgtacg
     7861 ataaggattt tcattttttg aatgcggtaa cagagtctgg cgctcctttg tgttatgtag
     7921 cggttgatga agcacgtcaa ttactatacg gggcgaacta tcataaaggt gaagtaaatg
     7981 tctaccatat attaggagat ggcgaactga aagcagcaga ttcaatattc catcaagaag
     8041 cgacaggtcc tcataaaaac caagatcatg cgcatgtcca ctatacggat ctgactcctg
     8101 accaacgatt agtcgtatgt gatcttggta cagatcgtgt gtatacttat gatgtgtccg
     8161 aaaacggcaa attgaatctt gtaactacct tcactgcaga gccaggaaca ggccctcgcc
     8221 atcttgtctt tcatccaaat ggacaattcg cttatttatt tggtgaatta gacagcacag
     8281 tccgcgtgct ttcttataat aaagaaacag gtgcttttga agaattacag aaaatatcga
     8341 ccttgccaga agaattcgac ggtgaaaatg gaggggcagc tatccgcatt tcaaatgatg
     8401 gcaagttcct ttatgcttct aaccgaggac ataactcgat tgctgtattc gcagtttttg
     8461 aagatggttc ccacattcaa aacaagcaaa tcatctcaac agaaggggat tttccccgcg
     8521 atttcgcact agatccaagc aataattttg tcgtgtgtgc caatcagaac accgataatc
     8581 tgacattata tcgccgtcat cctgaaacag gattactgga attactgaaa aaagatgtct
     8641 atgtaccaga atgtgtctgt gtttattttg atcgttagta agaaaaggag aatgaaatga
     8701 atcagcaaga cgtattagac agactacggg aagaactaaa tattcctttc tttgatggga
     8761 aaatcgaaga taaagagtat agcgaagagg aatatcagaa actcaaagcc gatttacaga
     8821 actactttga acaatatgta cagaatgtag aaaactaatc agctttccat actatctgaa
     8881 aagaacatca ggacaagact tttccgtcta tcctgatgtt cttttctaca aataaaaaac
     8941 cgccgagatg gttagactcg acggaaaagg agtatttact cgattttgag taagtacaag
     9001 gttgttgttg gttataggta tgtcattatc ataaaggatt ttgatcactc tatcataaag
     9061 gtaatattac atttatatta gataactcta gtttttattg ttatcaaaga tttcttttat
     9121 tttttcaaca acaaattagt cattttttgg atggcagctg tcaattggtc tgtcgcttgg
     9181 tcttgttttg cttcagatgt attgaataga tcttcatcta cccctgattc aagatcgata
     9241 ctttggatca tttcttcaca gcctgctaca tatgaaagat aagctttttc aaaattttta
     9301 tgaatgccca ttgcttttgc tggtggacgc aaagtactga ctttcataag cattgcccga
     9361 tatttttttg ttccttcttc aaataacagg atgatctctt caagagtcga tttgtccagt
     9421 tcacttgttt ttccttcatc aattgcgttt ctgatcactt catatttcgg atgcattcct
     9481 tctccgattg cttctgtttc cttgacgatt tgatcgatgg actgcacata atagccaata
     9541 ttcggtaaca tgttcgtttt cctactttct ccattgttgt tattatttat tctacttgtt
     9601 tttatcctca aatgacaagt acttgaaaaa atttaatggt ttctatacag ctcgccattc
     9661 ttattcgtgc tcaaagaaac catctgctaa actatattgt ttccctgcga caatgtcata
     9721 ttggatcaat tgatattcgt cgtaaatctc ttgttgcgag cgattgaatt gcgcttcttt
     9781 ataccactgt ccgttttgat agtacagttc tcgttcaaac gccggaagtt cctgttctaa
     9841 tgataaaagc agctgataaa agccatttgt tgtctgattc gtctgttcca ttagattcgc
     9901 tgcaaaataa aacggactgg tgattgcatc gtgagtcgtt tgcttttcta attctccttt
     9961 actgtcgaac ataagaaatt gtgtttcatg caatgtttgt ttgtcatttt ttgcttggat
    10021 cgtatctgaa tagatccctg gaagatgatc cccccaaaaa acaactaaag tccttctaga
    10081 aagattcttc aattcttccg tgaactgctt cagtgccgtg ctgctatagg aaatatcttg
    10141 cagataattc tctaagtcaa gtgtcccact tccatctgaa gtttttgcag gatagtctag
    10201 attatcatac tttccatcgt agggcatatg tgtctgcatc gtgactaaat gaatgaactg
    10261 aggtgttttt tcttccttga gaagtgtcat tacttctttg taagctgact catcagaaat
    10321 atatggatta ttctctatcg tatccgtata ggtcatcgta cgctcgctga tgaactggtc
    10381 aaatcctaat gtctgataaa catcctctct tttatacatc gaagtattgt atggatggat
    10441 cgctgtcgtc tggtatgatt gtgcgtttaa cgctgaaacg atagaaggaa gctgatccat
    10501 ttttggtaca agcatcgtgt aaggggtggt gagttgcgca ttaaataacg ccatagaaaa
    10561 accagtcaat gcttcaaatt cgatatttgc tgttccaccg ccatagtttt gagaaagcat
    10621 attgccacta tatgtttgat cagctacttc ataataatct gccaatggct caccagatac
    10681 ttctacgccg tttaatcgac taggatctga gaaactttca ctcatcacga acacgatgtt
    10741 cggcgactct tcctctacag ttttattttt ttcatcggct agcttctggt atttttcagt
    10801 gatttctttg atcttttctt tcgaatatcc ttcaggttca tccattggtt ctaccttcag
    10861 attgtacaaa aacccaccga taaaaccagt gttatagtag ttcattttct ggctgtaagg
    10921 tatccatagt gccgttttat tatatgcttt tcgcaacaaa ttatcgggat tgttgaaatt
    10981 actgatatac cctagcagtc caattgccac aagcagtgtc gttaatcgga tcaattggaa
    11041 tcgccttcct tttttcaggc ttcgaaacat gtaccagcaa aacaatccta tcacggttcc
    11101 tgcggcagct aagatcactg taaacagcat agtgccggtc atatctttca gcaatccgat
    11161 ttcagtgatc atcttcaaat cgtctggata aatcggttct tgccgataac tcattttcat
    11221 ataatttgca aaacctaata caccgatggc tacgacatac gtaagggaac ctaaagggac
    11281 agatcctgct agtgcaatca gaaaaataag aataatcagc aagacgagac aggctaagaa
    11341 gaatttttcc gtatgccaag aaaaagcaaa tttcaacgca aggtctacgg atagatcatt
    11401 ttgacaccac tgcaaaaata aattactaaa aacaacaaga aaaaagatac tgatgattgc
    11461 tgctgttcct tttaaaatct ttttctgttt ggtcgaaagt ctgcttatat atcttttcaa
    11521 atgcttgctc cttcatttct atcaataaaa gaatgttgct tctcttattt tagttgtacg
    11581 aaattcgaaa gtaaagaacg gaaagaagaa ttaatgaaat ccaaatattc tctttcttta
    11641 tggcctttct ggtttcttta ataagcggac ctttcgtact ccagaaattt tttctagctg
    11701 ttgtttcact tgattgagct gttcaaaatc agtactgtct atgtcaatca atatgtagac
    11761 atgctcattc ttatggttac gcgatatatc tgcgatcgct agctcgttct cattcagcag
    11821 agaaaatatc tctgctaata tattggtttt cttttgataa aatatagtga atcgaaacgg
    11881 cgcttggaaa aacattcgtg cggatggata attgaccgct tctctaaccg taccgaacaa
    11941 aagataatct tttaaggctt caactgccaa acgtccgctg tctgccaaag cttcctgagt
    12001 cgatccccct agatgaggca acataagtat acggtcattt tctaagaact cttcttctgg
    12061 aaaatcagtc aagtatttag ccagatgttg tttttccaat gcttgcataa cagcatattt
    12121 atcaacaatt tcacttcttc ctacattgat aagtacagca tcttttttca ttttttctat
    12181 attcgcctga tcgatcaatc cttttgtatc ttctgtcaac ggcagcatga ctacgatgta
    12241 atccgaggta gaaagaactg tttctaaatc tgcttgccga acataatcca actgggcatc
    12301 gcggatagaa taacccaaga catccatccc taagctatag caacttagcg ctacttttgt
    12361 gcctatcgct ccaagaccaa gaagaccgat ggttttgcct tgcaattctc gtcccacata
    12421 agcacttctt ttgttttctg cctgttctaa aatattagga cctgttagtg tttgtaccat
    12481 acgacttgct tcgattatcg gacgggaaga taagagcaaa cagcacaaaa caagctcttt
    12541 tactgcatta gcatttacac ctggtgtatt catgacgatc gtcccgtttt cagaagcttt
    12601 ttcaacgttg atcgtgttca ccccaacacc agctcttgaa attgctagca aatctggtgt
    12661 aagccattca tctttcactt tcgtactacg aataactatt ccttcggctg gcaaatcatc
    12721 tattattttg aaatattccc cttgtaactt ttctatctct tgttctggaa tagcatcaat
    12781 caaatgaacg tgatacataa aaccccctcc ttactaacaa gtatctcgaa atttctgcaa
    12841 ttagacaact gtttaccatt tgacttccta gaagcaaaaa gagcagttag agcagagtag
    12901 gctgagtgat aaaaataagg ctatgacaag ttactgccat aagccttatt ttcgagagca
    12961 aaatattgag ggtgagatat cttgataaat ggtgttccgt tagctgacat tgataataag
    13021 actaaatttc aaaaaagttg aaaagcaaat ttcgtcaatc tcttcttatt tcttaaaact
    13081 aagtggcttt ttcacaacct ctttttatct aataaccact gtttccaact caactaattt
    13141 tgcccacgtt tcaatttgat ctgttgttag atttagcgag acaacggtat gatgaccgcc
    13201 acctgcctga atccatgaat ggacaccttc atggaaattc ggtttagttt tccataacac
    13261 acgtgcaaca ggtaaattag gtgcttcagt cgttggttca aaggcttcta cttcattgat
    13321 cagcagttta taatgcgtac cgaaatctgc catcgatacg actacccctt ctcctgcttt
    13381 cccatcaaat acaagacgcg ccggatcttc tctatctccc attgataaag gagaaacaac
    13441 gattcttggt ttcgtcgcag caagcgtcgg atctacttcc atcatatggg attccaagat
    13501 tgcttcttgg ccactagcta gctcgtaagt ataatcttcc atgaatcctg tatctttccc
    13561 tcgagccatg attttcagca accgatcaat cgctgcagtt ttccagtcgc cttccccagc
    13621 aaaaccgtat ccttctgcca tcaaacgttg tacagctagt ccaggtagtt gtttcattcc
    13681 atacaggtct tcaaaattag tcgtaaaagc gttatagcct ccttcttcta agaaacggcg
    13741 aataccgatt tcctgctgag cttgtacttt tacatgttcc tcccatgtct tttcttcata
    13801 atcaccatag tcgaaggtat acaattcctt gtactcttca aataattgat cgacttcttc
    13861 gtctgatacg cgatcgatta cttgtacaag atctccgata ccaaaataat caacagtcca
    13921 accaaattga atctgtgctt ccactttgtc tccttctgtg acacccacgt ttctcatgtt
    13981 gtcaccaaaa cgagccactt ttattccaaa actttcatta taggctactg caacatccat
    14041 ccaatcagca atctcttttt ggatctcagg tcgtttccag tagccaacga cgattttatt
    14101 ttgtttattt aatcgagcat tgatgaaccc atattcgcgg tcaccatgag cggattgatt
    14161 taaattcatg aaatccatat cgatcgtttt ccaaggaatg ctttcgttat attgcgtagc
    14221 taaatgaagc aatggttttt gtagaagctt tgtcccgcgg atccacattt ttgctggtga
    14281 gaaagtatgc atccaagtga tcacacctgc tacttcttct cgataattga cctctttcat
    14341 gatttttgtg atcgtatcag cagtaactgc taattcttga aggcggattg gataaggcaa
    14401 ttggccattt tcattcagtt catcgaccat ttcttgtgca tgtttcttta cttctcgcag
    14461 tgcttcttcc ccatataaat gctgtgatcc tacaacaaac caaaattctt tttctccaat
    14521 attcaacata attagccact ccttttattt gttttgtccg taatacgcat tttttccgtg
    14581 ttttcgtaaa taatgtttat ctaaaatccg ttgaggcagc tcttctgaaa atgaattgag
    14641 ctgtctggta aataaattca tcttgcatac ttcatctagt acgactgcgt tcattaccgc
    14701 actttgcgca tctttccccc aagtaaatgg accgtgtccg tgtaaaagta cccctggaat
    14761 tgctaaggga tcaattttgc gttctttgaa tgtttctatg ataacttttc ctgtttcata
    14821 ttcgtacccg ctgtcgattt cctgttgtgt caaaaatcga gcacaaggta ccgatccata
    14881 aaaagtatct gcatgcgtcg tccccatcgc aggaacatcc aatcctgctt gtgcccagat
    14941 cgtcgcccat gtcgagtgag tatggacgat tcctccgatt tgaggaaatg aacgatataa
    15001 aacagcatga gtaggcgtat ccgaagaggg attgagttta ccttcaatga tatgattgtc
    15061 caagtccaca acgaccatgt cgtctgctgt catctcttca taactgactc cgctaggttt
    15121 gataacgaaa tatcgtgttt caggatcaaa agcgctcaca tttccccatg tatatttgac
    15181 gagtccgtgt ttaggcaagt caagatttgc ttggtatact tcttctttca gttgttccag
    15241 cattttctat cactcctttt tcttgcaatt gatgaatcgc tgttttttca atgtcgatcc
    15301 cctcacggta acgtctcata aatatctgat agcctgctat ttcttctgct gaaggtgtga
    15361 tggtgattcc ttggtcttgt ccaaacacgt cttctgccaa gaagttttct aaagacttcc
    15421 catgtcgatc catcatatag gctgctaata acgcgattcc ccacgctcca ccttctccgg
    15481 ctgtgtccat cactgtaact ggtgcctcca tagcagatgc tagaatccgc tgtccaactt
    15541 ctggtgtttt aaaaatcccc ccatgcccca ctaaacgatc aatttggaca ttctcagatt
    15601 tcaatatatc cattccgatt ctcattgcac caaaagcact ggctaaatgg atccgcatga
    15661 aattcgctaa atcaaaacgg ctacctggtg tacgtacaaa taatggcctt ccttcattta
    15721 ctcctgtaat gttttcaccg gaaaaataac cataagaaag taatcccccg caatccgtat
    15781 cgccttctag cgctttgttg aataataccg tgaataattt atctctgctt atctccatac
    15841 ccaaagaatt ggtaaattct tcaaaaatac tgacccaagc attgatatcc gaagagcagt
    15901 tattcgtatg aaccatgcct actagttctc ctgaaggtgt agtgaccatg tcgatttccg
    15961 gatggacatt ttctagttct ttttctaata cgatcatagc gaaggcggaa gtaccagccg
    16021 aaatgttccc tgtccctttg gctacactgt tcgtagcgac cataccggtt cctgcatccc
    16081 cttctggcgg acaaaccggg attcctggtc tcaaatttcc cgtagggtcg atcagataag
    16141 cccctgattc gcttaacttg ccagcttctt ctcctgctac tagaacttta ggcaaaatgt
    16201 gatccaagtc aatcgctacg tgcgcttttt cgattaactg accaaaaata tttttcattt
    16261 cttgatggta ggttttcgtt gaaggatcga taggaaacat gcctgatgca tctcctacgc
    16321 ctagtacttt ctcaccagta agctgccaat ggacataacc tgcaagcgtt gtcaaaaaat
    16381 taatttcttt cacatgttct tctttattca aaatagattg atacaaatga gcgatactcc
    16441 atctctgcgg aatattgtat tgaaattttt ccgttaatat tcgttctgct tcttcagtga
    16501 ttgtatttcg ccaagtacga aatggtacta gtaactgttc cttgctatca aatgctagat
    16561 aaccgtgcat cattgcactg aacccaatcg acccgattgt cgtaagattc tcgccataga
    16621 tttcgaatac ttctgctgcc atttttcggt aactgacgcg cagtcctttc caaatatctt
    16681 ctaatgaata ggtccagata ccattttcta gtttgttttc ccactcataa ctaccagcgg
    16741 caataggagt aaaagatgaa tcaatcaata cagctttgat cctagttgat ccaaactcaa
    16801 tccctaaaga tgtacgacca tctatgacat ctaacatact ttgcgcattt acttccttca
    16861 taatgattta actccctcct tctcgtcaaa cctgatcaaa ccgtcttttt agtagcgctt
    16921 acaatgatta ttgtatactt ttgtacgtac atgtcaagca gttatttaca agaatacaat
    16981 aaaaaacact tgttttgtac gtacataagt tataaaatga aattgcttac aaaatctgat
    17041 caaccgcctt ctcgaaattc tagaaggaga gaatacttat gaaaaaagaa aaaaaagttt
    17101 cctcatcgtt tatttatttt tttggcgcat ttggcggcat tctatttgga tatgatattg
    17161 gtgttatgac cggcgcctta ccttttttac agcatgattg gggtttggca ggtaaagctt
    17221 ctttgatagg atggatcacg tcttcggtca tgttaggtgc gatccttggc ggctctttat
    17281 ctggtcttct ttctgataag cttggacgac gaaaaatgat tcttctttct gcacttattt
    17341 ttatggctgg ttctgtcctt tctgcatctg cccctcacaa tggttcttat tttctgattg
    17401 cagcaaggat tcttctaggt ttagctgttg gcgctgcatc tgccctagtt cctgcttata
    17461 tgtctgaaat ggctcctgct agactaaggg gaagattgtc agggatcaat caagtgatga
    17521 ttgcttctgg catgctcctt tcctatgttg cagattattt gctaaaaggg ttgcctgaaa
    17581 cgatggcttg gcgcgtgatg ttagggctcg ctgctgtccc cgcgctgatt ttatttttcg
    17641 gtgttttagc tctaccagag tcaccgcgtt ttcttatgca atctggccgc ttggaagagg
    17701 caaaaagagt attgaactat attcggacac caaaagaagc tgaacaggaa ttcgaacaaa
    17761 tccagctgaa tgtcaaacaa gaaaagacaa ccggaacttc ttggcatact ttgtttttag
    17821 aaaaatatcg ttccttagtc attgccggta ttggtgtagc agtattccag caatttcaag
    17881 gagctaatgc tattttttac tatattccgc tgatcgtaga aaaagcgact gggcatgcag
    17941 cgagcgatgc attgatgtgg ccgatcatcc aaggaatcat tttagttgct ggttctttgc
    18001 tctttttggt tattgcagac aagttcaatc ggcgaacttt attgaaaata ggcggtagcg
    18061 tgatgggact ttctttcatc ctaccagctg ttttaggaac tgtactggat gctcatacaa
    18121 attcgttgtt gattctcttg tttttatgta tttatgtcgc attttattct tgcacatggg
    18181 ctccactgac atgggtaatc gtaggcgaaa tattcccact agctgtccgc gggcgagcat
    18241 ctggcctagc ctcttctttt aattggattg gttctttcct agttggcttg ctttttcctg
    18301 tgatgactgc ttctatgtct caagaaatcg ttttcggcat ttttgggatc atctgctttt
    18361 taggggtttt atttattcaa gagatcgtac cagaaactcg tgggaaatct ttagaggaaa
    18421 tcgaacaaag tgcatctaaa aaaacttatc ctaaacgtat ttctatccat cattcttaaa
    18481 gaggttgtga cagaagtggt cagcttcaag aaataagaag gaaccgctta gtttccagtc
    18541 acaaggaaca atttcaaaaa acagcttctc atgtttgatg aatattgtga cttgtgacca
    18601 gttggtagga gaacaccgtt tagtggagag gtggtgaaag aaacgttcag ccttgagcga
    18661 tgacaaaagc tctcacaaac attgcgacga gtaaccgtag gagcaataag aggcggtaaa
    18721 aacccgtctc ttatttttcg ttttctttca ctgtttcccg ttcaactagt acaggttcat
    18781 agagaatatt tggctgtgcc tgtccttttt caatcgttgt gacgatccac cgtgcggcat
    18841 cttgtcccaa cttttctttt ggatgcgtca atgttgtcaa ttttttattc cctaaatggc
    18901 ttaaagaaga atcgtctgtt cctacgatcg ataggtcatc tggaatatga tagccatttt
    18961 ctgtcaactt atctagcatt aaactagcta tttgatcgtt atagcaaatg atgccatcta
    19021 aagcttgttc ttctttcaac cgttgtactg ttttttcagc gattgtttct cgtgtttccg
    19081 tggtgaaagt aatgatatct tccgtcaaaa aacgcacatt atactgttca cacgcgctga
    19141 taaaaccttt catcctgtat tttccttgaa gatcgtcgat ttttgtgatg aacaacagac
    19201 gctgatgtcc tttttttatc aaatattctg ctgccataaa accgactttc acatcatcta
    19261 aacaaatata agaggcattt aactcttcat aagcagcatt gatcatcacc atcggaatgc
    19321 cttgttctct caaagcaaca taaagtgcta agtttgggtt atactgatta cttttcgtag
    19381 gctcaacgat caaaccatct accccataag caatcatttt cttcaaacac tctttctcca
    19441 attcgtgatc gttattcgta ctggctaaaa gcaatgaata cccagattct cgtaattcat
    19501 tttcgattcc tcggataatc gacggaaaaa tataatcaga gagatatgtg gtgatgacac
    19561 cgatcgtttt tgttctgttc aatggtgttt tttctttctt atagctgtca tctacatatg
    19621 tgcccgaccc tttttcttta cgcagatagc cctcatttac caacacagcg attgcttgcc
    19681 gaatcgtatg tctgctgacg tcatatattt cttgcagctt gaattctgag ggaatggttg
    19741 tatttacttc gtattcctca cgaaggatct tttgttttat ctcttctgca atatcttgat
    19801 attttgccat ttgccttgcc cctttttatt tgtacgtaca tatatcataa cgagtctgca
    19861 aggtttattc aagcaaaaat caaggtttct tgtgctcata aggcatacgg aacaggttca
    19921 ccaaattctg gaaatccttg ctcattccac gtaaagcatt gcgcgttcgc gtgccgatta
    19981 ggattgtcta acggatcccc tttttggttc ttctctggtc gagcatgata gatcaaaata
    20041 tcaaagcacc cgtcttctga aaccgtaaac gagttgtgac caggaccaaa ttgcttattt
    20101 ttttcagaag aaacgaatac tggctccgat aatttatgcc atgattcttt taataaaaga
    20161 ttcttgtgct catcagccca cagcaggccc atggcatagt tttcatctgt tgcactacca
    20221 gaatacgtaa tgaatatttt cccattgcga gtaaggactg ctggaccttc atttactgaa
    20281 aaaccccgtt tttcccattc atactcaggt atagaaagga ggatctgctt cccctttaac
    20341 gtccaaggat tatccatttc ggatagataa agattagaat tcccagtaat ttcaggatct
    20401 tgttgggccc acacataata tagctttcct tgagaagaga aagttgttcc gtctaaactg
    20461 aaagtttccc ttttcgtttt tatttgtccc ttttctatcc aatctgtatt cattggatct
    20521 atatgctgat tttcaagtac gaacatgcgg tgatgatgag attcatcacg tatagtttta
    20581 tgattgcttg cagcaaagta aatataccat tttccattga tataatggac ttcaggtgcc
    20641 cagatcaatt cactcatcgg tccttcttca tgagcctgcc atacaatcag gcttgcagca
    20701 tgttctaatt cattcagtga ctttgccctt cttaattcaa tcacttggta gcctggaacc
    20761 gatcctgtaa aataataata accatctgta tgtttatata cccaaggatc tgcccgttgc
    20821 cgaacgatag gattattata aagtgtcttc atcataacaa atttcctcac attttcttta
    20881 ttctaattac attccaagag tatttttcta tccctacatt taattcgtca tgtttttcta
    20941 ttgaaaaata gtttatttca ggttttattt tttctggttc ttcacgtgta tttttgtcca
    21001 acagatcgct actggttaat gtgtagtgct ctccttgatc tagcaaattg aaatcatgtt
    21061 caatcgttat cttttgttgt tcatttgtgc gattgatcaa gaaaatagtc agctgattgc
    21121 cgttatctac gatcacactg tccacataag gaacattttt tatcttttca caatcataaa
    21181 cagggcaaat gatttgttgt ttcaatactt gacctttagc atatttcgat aagtgttgca
    21241 tcacataata agttggattc ctccatgctt gtcctttagc atctgtcagt attaaaggta
    21301 ttgtgtttac taacaatgat tgacaactaa tctttacgcg atctgcgtgt cgtaaaatag
    21361 ccagccctaa tgaaccaaat aataatgtat cttccatagt aaagtaacac caatcgatgg
    21421 gactaccgac ttcaaaatcc ctatgtcttt tttccggttg ggcatgtaca ttccattcat
    21481 caaacgctag atacattgtt ttctcacttc tcaaatatcc ttttacataa tcacaagtag
    21541 caacgatttc ttcaatttga cgatcgaatc gataagcttt tgcaaggtat gtcgctgtat
    21601 catcacgctc atttttagga ggtttagtca agtcttcttc atcatactta tcaatatagt
    21661 tatgcaaagc taaataatct acattctcat aagcttcttc taatgcgata cgatcccatt
    21721 cagggtacga tggtaaacgt ggcgtagagc ttccaactaa gattgtttca attgaatcat
    21781 ccactaactt catgactttt gaggcttcat ttgctaacct accatattca taagcactct
    21841 tagcccccac ttgccatgga ccgtctagct cattccctaa acaccataat ttaacgccaa
    21901 aaggcttttt ttgcccattt tttattctta aatcactcca gaaggttcct tttggaaaat
    21961 tacaatattc tactaaattt tttgcttcat caattccttt tgttcctaaa ttcaccgtga
    22021 atatcggttt cgcttgtaca tgactgatcc atttaaaaaa ttcgtggagt ccaaaagaat
    22081 ttggttctat acctcgccat gctaaatcaa ttttttttgg tcgttgttct ttcctaccta
    22141 cagtatccac ccaatcataa ccagatgtaa agtttcctcc aggatagcga atgacaccta
    22201 attctaattc ctttacataa gagaggacat cttgtctaaa cccgtcacca tcagctgttt
    22261 catggcccgg ttcataaatc cctgtataga cgacacttcc catatgctca acaaatgagc
    22321 caaacagccg agaactgatc tctcctaccg taaaatcctc tctgattttt actttcactt
    22381 cttcgtccaa cagctttccc tacctttcat ttcctatagt caagataata gatacaagtt
    22441 tccatacgcc accagcaaag ataaaaataa aaggagggaa aagaaatatg agcagtacaa
    22501 atcctaataa caatacgcaa caaagaatat ttctcaaaaa atcaaaaaat actttccatg
    22561 cactttgttc aaaaatttgt aaaaatgtga tatctttgtt ttcagaaatc acatatatcc
    22621 aggataaaag aaacatggaa taaataagta aaactacata tccactaatt gaaagtacga
    22681 ttgtctgttc ttctaataat tgattaacaa gaaaataaat aatgatcgtt aacagactaa
    22741 ggaacaagtt cttaccaaaa tgttgcttta ttccttgaaa gaaaaaataa atgatatttc
    22801 ctgatccctc acctttccat tgcgccatta cataaaaaac agcacatgta gcaggcacga
    22861 tagtaataac aggcaaacac acgattaccc agagaatacc taataagatg taatcagaaa
    22921 ccacaatcaa ctgtccataa agtttagtct caactatttt catcttcctc actctttcat
    22981 tccagacata gctgttcctt gaacaaaata tttctgtgca aaaatataaa ccactagtaa
    23041 tggcagcatt gaaataaccg ttccagccat tacaggtcca taaaaagaaa tgttctgccc
    23101 tgtaaataaa gctaaccctg ctggtaaagt tctcattttt gaactacttg tcaatatcaa
    23161 tggataaagc aagtcattcc aactattcat cagcgtgaaa atccctaatg ataaaagagt
    23221 aggtttgctc aatggcaaca tgattttcaa aaaaatatta aactcactta atccatcaat
    23281 gcgtgctgcc tcttccaaag aatctggtaa agtaataaaa aaggaacgca tcatgaagat
    23341 accaaatgca gttgtcatac gaggaatgat caaacctgca taagtatcaa gcatgccaag
    23401 caaattgact tcaataaata gaggaatcat gaagacttgg aatgggatca tcattgtcag
    23461 caaaacgaaa tagaacaaaa ctgattttcc tttgaaacgc atacgagcaa atgcataccc
    23521 cgcaagagaa tcaaagaata cagaagtgat cactgtccct ccagcaaaaa tcactgtatt
    23581 tttgatgtaa tctaataaag ggatcgttgt ccaaacctta atatagttat ctagggtata
    23641 gctatctgca aatattctag gcggaaatga gataatgtct ttttcgtgtt taaaagaaga
    23701 aataatcagc cacaaaaaag gaaacgcaat aaccaacaat ataactagca tactaatcgt
    23761 tcgaatgatt tttttaccac taactgcttt tcgactattc acagtaactg taatcgagct
    23821 ttctggtgcc atacaggttt ccctcctatt tcatttgttt ttctttccgt gacatgtaca
    23881 cattagtgga gatagctata gttgcaacaa tgataaacaa aaccgaagac agtgctgatg
    23941 catagcccaa ttcataaggt gctgtaaatc ctcgactgta aatatattga acagctgttt
    24001 ctgtcttaaa ttgtgggcca ccagcggttg caacatatac ttggtcaaac acttgcatcg
    24061 aaccaatcag attcgttaaa atacaaaaac ctaaggaggg aacgatctga ggtagcgtaa
    24121 tgttaaaaaa ctgctgtcga tttgttgcac catccatttg agcagattca tacaaagagg
    24181 gagagattcc ctgaatagca gtcaagagaa tcgtcatagt aacaccaaaa ttcttccaaa
    24241 cagtcatgat ggctaccgtc ggcatagcaa gggttggatc gcgaaaaaat tgagggatcg
    24301 gcaagccgac ttgtgaaaac aagtaaggaa tcgcccctac attcggatca agcaacatag
    24361 aaaaaataat cgcaattgac gtcaatgaac aaataactgg aatgtaaaat gtcgaacgac
    24421 aaaaacgatt gaatctggta tttttagata atgctactgc tgctaataaa ccaacgataa
    24481 tttgtgttgg cgtttcgagt acggtgaaat ataaagaatg aatcaacgaa ttaactgctc
    24541 gttcatcact aaatatacgt gtgaaatttt caaaacttac aaaatttctt gatgtaaaga
    24601 aaatattcaa atctgtaaaa ctaatagcaa atgttcctaa aagcggtatg acagtaaata
    24661 ataataaaac taaaaaagat ggagttaaaa acaaatacgc agcacgatca ggacgattcc
    24721 aatatttggt gaatccgttc atatagtcca cctcatcttt cattttgata aaatatacaa
    24781 tcttggttta aacgaatctg aataagatat ccgaacaatc gctgtgattt ttgcaataac
    24841 acctatctgc aaatactagg tcgatcattc agatatctgt atcttgccaa cagtctatcc
    24901 tgtataaagt tctgtactat ttttgcagta attgattgat ggcatcatct gcatcgttca
    24961 tgagtgtctc aggatcttct cctgctaata atttttcaat cagtggattg atgatatcgt
    25021 tattgatcgc agagatttgg gtaaaacctg gtaaatatgg ctctgcatag tccatttgtt
    25081 tcgataattc agatacaata ggatcatttt ttacttcttc atcttttgct actgaatcca
    25141 agtaggcagg aaaaccattt tcgatactcc attttttacc aatttcagtt gtattccaat
    25201 atttcaaaaa ctcatagaca gcttcttttt tattattatc tgtacttgcc ggaatggcat
    25261 atcctacgcc atctaaaata gctgtttttt tgtctgaatc actcccttgt ggtaaggtag
    25321 tcacattata atcaatctcg tttgctttca atccgttatt taaccatggc ccattgattt
    25381 caatggctag ttgaccagca ttcattaaat tgtccatttc ggcgccagaa atcgattctg
    25441 gaccagtctt gtctacatgg atcatttttt ggatcgcttt taacgtttta aggttctcag
    25501 gagacgcaaa ttcagactta gtataatcat cgttcactaa tttaccgcca tttgctaaca
    25561 tccagttata taacggtgca gttccatcct tattgaagac aaaacctgat acatttttac
    25621 tactatcagt taattttggt gccatttctg tcaattcttc ccaagtagta ggtggttgtt
    25681 cagtatctaa tccagcttgt ttaaacaaat cattattcca atacatatac atgccttgta
    25741 cctgcatggg gacaaaatac tgtttacctt ctacttcacc taattcaata gccgtttctt
    25801 taaaatcaga tttatctact ccatcatatt cccagaaatc gtctaatggt tgtaccgctc
    25861 cgttttctac atactgtgca aaatctggat aatttaatgc aataaagtca ggtgcttttt
    25921 tagaagaaat cgctgtagga agtttttcat tcaaattggc ccaagtcatg atatccattt
    25981 caattttgat gtttttatca tttgttttat taaattcatt aacgatttct tgcaatattt
    26041 caccatcaga tgcagtaaac ccattccaaa aagtcaaagt cacttcttta tcagaatcag
    26101 attctgctga acttccgcca ccgcaagctc ctaaaatacc tgttgcagct accaacaaag
    26161 agccaattaa ccatttttta cctttcattt tttgttcctc cttcatctga taaacgacat
    26221 ctgaccacat tccaagatag cggtttcaat aaacaattga ttttcccatc ttcagctttt
    26281 acatttgttg aatgacttgg ttttacttgt tcacttttag gaagattagt tgcttttgta
    26341 tcaaatccac tcatttcaga aaaatcaatc actccctcta atgataattc agacaagtga
    26401 acagttagat tcatttcttc ttctccacgg ttaactgcaa atatgacaag ttctttgtca
    26461 tttattacgg ctatactttc taaatacggc acttcttcaa aatcttccgt cgcatagttt
    26521 gctgaacgaa tttgtgggct taacacctct ccttgtccat acacagcagc ttgcataaat
    26581 ggataataga tcgtctgtct ccaagcttct ccgtttgttt ctgtcctgat tggtgcaata
    26641 acatttacca attgagcaag acaagcaatc tttactctat ctgcatgttt caataaagtg
    26701 attaatagac agcctaatag taatgcatct tcaaaattat agttatcttc tagtaaagga
    26761 ggagcaatct gccaaggcgc tgcttgctca tcttgttccg ctgaatgata ccaaatattc
    26821 cattcatcaa aggacaggtt gatctgcttc ttactatgtt tcttcgcctt gactgtatcg
    26881 cacatcgctg tcacttcaga aatgaaccga tccatgtcta aggaacgtgc aagataatga
    26941 gaaagatcat ttttttggtt gccataatat tgatgcagag acaaataatc tatttgatcg
    27001 tacgcttgat caagaactgt taactcccaa tcaccaaatg taggcatttg ccgattcgaa
    27061 cttccgcata aaaccacctc aattgaatca tctacgagtt tcatcgcctt agccgtttcc
    27121 tctgctaacc gtccatattc ttccgctgtt ttatgtccaa tctgccaagg accgtccatt
    27181 tcattcccta aacaccatgt cttaattcca aaagggctct cttgaccatt cgaacgcctt
    27241 aaatcactcc aataagttcc tttaggaaaa ttacaatatt cgactaaatt acgagctgca
    27301 tctactccac gagtacccag attgacagcc atattgattt cggcccccac atgctgacac
    27361 cagttataaa attcatgaat cccaacttca ttcgtttcaa ttgtccgcca agctaaatcc
    27421 aaccttctag gtctatttgc tatgggccca atactgtctt cccaacgaaa tcctgacaca
    27481 aaatttccac caggatatct aatcatggga atgttcaatt cttttaccaa gtgaatgacg
    27541 tcttggcgaa atccactttt attagctgat ggatgatctg gctgataaat cccttcatat
    27601 actgctcgtc ccatatgttc aataaatgaa ctgtacaacc gcggatcaac tttgcctaca
    27661 cgcttgacac tactaatcaa cattgtggct tccataactg acctccttta atttatacat
    27721 gaataataca cctcgttgaa agcgttgtca ataatataaa tgttaaactt acttattttt
    27781 tatcgatttt aattaaccaa aatattaata atgattaaaa caaataattt aatataactt
    27841 ttattaacaa atatgttaat atataagaaa aaccttttaa gaggtgatat atatgcaact
    27901 acaaataaac gaagaatcct taccggtata tgaagcatta gctagtaaaa caaggatcaa
    27961 gatcatccag ctactttcca aaaaaaagat gaatgtcaaa gatttggcaa aagaacttgg
    28021 agtgagcagt gctatcacga cgatgcacgt gaaaaagcta gaggaagcaa acatcatcaa
    28081 aacagaaaaa gtaggacaac aaaaaatttc gagccttcgt gtcgataaaa tcgatatcag
    28141 ttttccagaa aaaatattca atgcttttga tacgaaagaa acatcgatac ctattggtca
    28201 ctatacaaat tatgcaattg aaccaacatg cggtttagct acgatccatg attttatcgg
    28261 gaaagtggat gaaccacgct attttatgga tcctcgaagg atggatgccc gcattctctg
    28321 gtttaccagc ggatttgtcg agtatcaggc acctaatttt ttaaatacag aggatacatt
    28381 ggagatgttg gaagtttctg tggaaatttc ttctgaattt cctttttcaa atgacaattg
    28441 gccttcagat atcactttca gcctaaatgg tgtggagcta ggaacatgga cgagccctgg
    28501 agattttgct gatatccgcg gtaaatatac gcctgattgg tatccagata atcttaatca
    28561 atacggcttg ctgaagacga tccgaattac caaacacttg acgaatatga atggagaacc
    28621 cttgtcaaac atcacgatca acgacatccc taaagagcaa gacacttggc atttgcgaat
    28681 cgaagtgaaa gacgatgcca aacatgtggg gggctgtact ttatttggta aaggattcgg
    28741 taattacgat caggatatca aattaaaagt ttactacagc tgatattttt cttttctatt
    28801 ttataaaaca aaaacagcca atccctatga agggacggct gtttttgctt tattgttatt
    28861 tccagaaatc atccaaaacg gaaattggca aatgtcgttt atgttgtgtc ttgttccacc
    28921 atgcttcgat tttttcttgt gcttgagcgg gaacttcttt tccttctaga taatcatcaa
    28981 tttcattata tgtgacacct agtgcgactt catctgcaat cattggtttc ccatcttcaa
    29041 gatctgcagt cggaattttt gtgtataatt tttccggtgc attcaatgct tgaagcaatt
    29101 gtttcccttg tcgtttgtcc aaacggaata atggcaaaat gtcggctcca ccatcaccaa
    29161 atttcgtgaa gaatccagta atgttttctg cagcatgatc tgtccctaat acggctcctg
    29221 ctctttctcc agctacggca tattgcgtaa tcatacgttg acgtgctttg atatttcctt
    29281 tgttaaaatc gctgattccc acacctgctt cgcttaatac ttgtacttgt gcatcaacag
    29341 ctggtttgat atttactcgc agactaacat cgggctggat aaagtcaaga gcagcttttg
    29401 catcttcttc atctgcttgt tcaccataag gaagacgaac agcgataaat tgatagtttt
    29461 catcttttgt ttcttcacgc atttcttcca tcgttagttg cgccaaacgg cctgctaaag
    29521 tagaatcttg gccgccgctg atacctagta caaaagtttt aagaaacgga tattttttca
    29581 aatatgcttt cataaagtca atacttttac gtatttcttc ttttggatca attgttggtt
    29641 tgacacctaa ttcattgata atttctgtct gcaggctcat ttattctcct cctatgcttc
    29701 tgtatgaagt tctgctacgc tttttttcat tcgttccatg atagccattt tatggttcca
    29761 gcattcagta gacagatcga ctgggtattt ttgcggattc aagtctcgtt tatactcttc
    29821 ccatagtgaa tctagatttt cttttgtata ttctttgatt tcgtttagtg caggtaattc
    29881 gtaaactctt tttccttcga taaagatatc ttgtaaaact gggcgagctg taaagtctcg
    29941 aacagtttta ttgataaacg tatgcactgg atggaacatg aagatagctt cttcttctcg
    30001 agggtcttca tcccataatg tcacgtagtc tccttcagat ttcccatcaa aatttcgagt
    30061 gatgcgccac acttgttttt tccctggagt agttactttt tctgcattgc ttgataactt
    30121 gatcgtatcc atcatttttc cttcatcatc ttcgatcgat actagcttga atactgcacc
    30181 aagggctggc tgatcatagg ctgtgatcaa ttttgtccca acaccccata catcaatttt
    30241 agctttttgc atctttaaat tcaaaatcgt tgcttcatcc aagtcattcg aagcatagat
    30301 ttttgcttcc gtaaatccgg ctgcatctaa ttgttcccga acacgcttag atatataggc
    30361 catatctcca ctgtcgatgc gtacgccttg gaagttgatt ttgtcaccta attctttcgc
    30421 cacgcgaatc gcacttggca cacctgattt caacgtatca taagtgtcga ctaagaacac
    30481 gcagtcttta tgggttttag cgtaggccat aaatgcttca taatcatttc cataagattg
    30541 gactaacgaa tgagcatggg tcccgctagc tggtatccca aatatcttcc cagcacgtac
    30601 attgcttgtt gcatctgctc cgccgatata tgccgctcta gttccccaga ctgctgcatc
    30661 cagttcttgt gcccgtctag ttccaaattc caatagtgga tcttctccga tgacagattt
    30721 gatacgagct gcttttgtcg cgatcaacgt ttggaaattg accatattca acagtgctgt
    30781 ttctactaat tggcagtgcg ctaaaggccc ttccacttgg attaatggtt catttgcaaa
    30841 aactagttct ccttcacgag cagaacgaac tgttgcttta aattcaaaat tttgtaaata
    30901 ctccaaaaaa ccttcaggat agacttctaa tccacgcaag taagcaatat cggaatcatt
    30961 gaacgtcaga ttctctaaat aattgactaa ccgttccaat cctgcaaaaa ccgcgtatcc
    31021 gtggttaaaa ggcatttctc gaaagtaaca ttcaaaaaca gcatgtaaat cagctcgacc
    31081 taattcccag tatgtctgca tcatattgat ttgatataga tccgtatgca gcgtcaaact
    31141 gtcgtctggg taagtattat tcatcttgta ttactcctct tggcaagttt tcatttccta
    31201 cccattataa caaaaaaaat taataggaaa agaaatcttc cattttttct gaaaaagcac
    31261 atgttttacg gatatttctt gctaaaaatg aactccatct gctaaaacta tcctttctta
    31321 ttggtattta acctaaacga gaaagaatac ttattttatt caatgtatcc gagacacgaa
    31381 gaaattagcg ttttctattt ttcttacttg ataaaataag tagattttcc tactacaatc
    31441 gtagccgtta agataccgat gattgaacaa atataaccaa atgtctctac aacataatca
    31501 atggcaaaag tttttgaagc aataatacgt ctttcctttt ttgcttctta tccccaaaga
    31561 aacggatata acttattatg tattgaattt ttttggaaag gcgaattatg acaagatata
    31621 ccttgcgttg aaaaaactcg cggaaaaaat atcacacgtt tttaaaaaga tacaaagtaa
    31681 gaaagcagct gtcaatagaa tggaaacaaa aatggcgaga catcaatcag tctcgccttg
    31741 ttttttattg tatacattag atatggagca ggttaccata aagatgatta ttagtcgtta
    31801 ttaaatagaa tccttcgtat ttttttgttc tatcactaca atatctttat ttggacgttt
    31861 gaattgtatt ccggcacgtt ctagctcctg tttcactcct acatggactt tgacatcttc
    31921 tcgtgtcatt tttaaaacgt gatgaatcgc atagtcgatt tcttgccttg tgattttttt
    31981 cttcgtattg atcatcacga cttgcttttt atcatatgtg tctaccaata gcagttgtgt
    32041 tcgatctaac gaaaataatt tgtaataatt aatagccgta ttcccaaaaa agaattccac
    32101 tactttagga taaaatgtct taatatttaa attactattc acaggtgttt cgattagctt
    32161 catctcattc ctcttttcta tatagtcgat ttagatccaa tcttaagtta tgttaccatc
    32221 tttcctagca ttaaggaatc ggttacaatt gaacctttaa agacaaaagg atttaccttt
    32281 tatcacttaa tagtataaca taatttcgtg agaaaaaaat acaatttttt cttgaccatc
    32341 tcgttacgta aaggattatg cttacatttg tcaggaggaa aaataatgga gtacacaata
    32401 aagaaaatgt cagagatatc aggagtcagt ccgagaacct tgcgctttta tgatgagatt
    32461 ggactgctaa aaccagctag gatcaattct tcaggttatc ggatttatgg caaaaaagaa
    32521 gtcgatcgtc tgcaacacat cttattttat cgaacaatgg cattcaagtt ggatgatatc
    32581 caagaggtct tagacaatcc atcttttgac caccagaaag ctttaatcaa gcatcagcaa
    32641 atgttgctgg aaaaacgggc acaaatcgat accctcttga caactgtcca acagacactt
    32701 gatatgtacg aaggaggaag aaaaatgagt gacaaagaaa aatttgaagg gtttaaacaa
    32761 caaaagttga aagaaaatga agaatcctat ggacaagaga ttcgacaaaa atacggaaaa
    32821 aaaaaggtta cagcatccaa tgaaaaatgg atgaatatgg ataaagaaac gtatgaagaa
    32881 atgcaaactg ttgaaaagca gatgttattt gatctcactc tttacttaaa agaaccagaa
    32941 gatgaaagct tagcagaccg tatcttccaa gctcataaga agtggttctc ttacagctgg
    33001 cctgaatatc agccaaaagc gcataaagga ctaggaatga tgtacgaagc cgatgaacgg
    33061 ttcactgcat attatgacga gcgaagtggt catggtgcag caaaagcttt aaatgaaatt
    33121 atccaaaaaa aggcgtaatc ttttacatga tccaacaaaa aagtacacgt aaaatgacct
    33181 aataacaaaa cagcagacac ccaagttaga tttttatgct aacttttggt gtctgctcca
    33241 ttctttcaca agttttttca tcctacttta aatatattca tcgttgaata aacgtataag
    33301 gtattttcac tttatccttt ttttctgtaa gaaacaattg aaatgccatt tctccgcaag
    33361 cttccagatg gtggtcgatg gttgggaaac caagaagctc gctgatcagc aaattatcgc
    33421 gtccaatcac cactggtgtc ttatcttgca gatagctttg caagatgcct gctgcaacca
    33481 tatccgaatt tgtaaagaca ccatcaactc caagctcttt aaaaaagaag cctgcttgtt
    33541 ttcctttctc tgcatctata caatcccaaa aaacaagtga ttcatcaaaa gatggaaaaa
    33601 gttgatgaca tagctgcaaa gtgatttttg agttataact tagcctgcca cttcgcccca
    33661 acgtgatgcc caattgcttt ataccttgct gcttcaaata agtaagacct tctgtaattg
    33721 attgttctcg atcaataaag acacagcttg cctgttttcc cttgatttct tcacaaaaaa
    33781 ctagaggtcc atatttttga tatgccaata aatcatctag tgtattggca cgagacgtaa
    33841 tgatcaatcc atcgaaagct ttagctgcaa attcttcaag ataccttcgc tctttttctt
    33901 tatgatagcc cgtcggtaaa agtgtcactt tgtaatcata atcaaacgct gcttcaatga
    33961 tcccactgat caactgatca aaatacggat gattggtata aggcagaatc actccaatgc
    34021 ttttcgtttt tccataactt aactgtctgg catttgcatt agggatataa tcgacttctt
    34081 tcatgaccgc aagtatttct ttcctttttt cctcatctac atatggatgt ccattgatta
    34141 cacgtgaaat agtagaaact gaataacctg tgagctttgc gatatctcga atgtttgcca
    34201 ctgctgcttc gccacctata caaaaagatt ttcttgtaaa tagtctacca cagctggacc
    34261 gcattcaaaa aactgataca tttttccttc atgcgaaaaa ctgatccgct gcccgcccca
    34321 atatatatct tttgtcaaat tttcgatttc attcattggt atttctttta ctatattcct
    34381 attcaaccct tgaaaactgt acaacgttaa tacggatccc ttgaactcga caaacgcata
    34441 aaaataattg ttcgttgtgc catctgttat ctctacataa gtattcattg caagccctcc
    34501 ttttgatact ttgagtatag tgtatgaaac gcgtctcata gcaaggagtt tgttttgcca
    34561 aagaaaagtt ttattcctct ttcattatta ccaaagtctc ttattttaaa ttgaatacat
    34621 tataatgata ctgtaaatat atctttatta ttagtgaatg gatattta
//
LOCUS       DACEUZ010000013        34296 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.013, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000013 DACEUZ010000000
VERSION     DACEUZ010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34296
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.013"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(<1..962)
                     /locus_tag="IXG44_000550"
     CDS             complement(<1..962)
                     /locus_tag="IXG44_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1657289.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHT"
     gene            complement(1261..1896)
                     /locus_tag="IXG44_000551"
     CDS             complement(1261..1896)
                     /locus_tag="IXG44_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1657290.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(2009..2644)
                     /locus_tag="IXG44_000552"
     CDS             complement(2009..2644)
                     /locus_tag="IXG44_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HAQ1657291.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            complement(2678..3139)
                     /locus_tag="IXG44_000553"
     CDS             complement(2678..3139)
                     /locus_tag="IXG44_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4429 domain-containing protein"
                     /protein_id="HAQ1657292.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQEEFDFKKKELLGL"
     gene            complement(3269..3700)
                     /locus_tag="IXG44_000554"
     CDS             complement(3269..3700)
                     /locus_tag="IXG44_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAQ1657293.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDK
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(3718..4038)
                     /locus_tag="IXG44_000555"
     CDS             complement(3718..4038)
                     /locus_tag="IXG44_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1657294.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            4337..5113
                     /locus_tag="IXG44_000556"
     CDS             4337..5113
                     /locus_tag="IXG44_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HAQ1657295.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            5128..5331
                     /locus_tag="IXG44_000557"
     CDS             5128..5331
                     /locus_tag="IXG44_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1657296.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            5347..5685
                     /locus_tag="IXG44_000558"
     CDS             5347..5685
                     /locus_tag="IXG44_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HAQ1657297.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            5672..5851
                     /locus_tag="IXG44_000559"
     CDS             5672..5851
                     /locus_tag="IXG44_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1657298.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(5894..6364)
                     /locus_tag="IXG44_000560"
     CDS             complement(5894..6364)
                     /locus_tag="IXG44_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1657299.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            6451..7149
                     /locus_tag="IXG44_000561"
     CDS             6451..7149
                     /locus_tag="IXG44_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HAQ1657300.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            7327..7668
                     /locus_tag="IXG44_000562"
     CDS             7327..7668
                     /locus_tag="IXG44_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1657301.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            7661..8332
                     /locus_tag="IXG44_000563"
     CDS             7661..8332
                     /locus_tag="IXG44_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HAQ1657302.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            8338..9024
                     /locus_tag="IXG44_000564"
     CDS             8338..9024
                     /locus_tag="IXG44_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1657303.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGG"
     gene            9027..9776
                     /locus_tag="IXG44_000565"
     CDS             9027..9776
                     /locus_tag="IXG44_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1657304.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            9788..10057
                     /locus_tag="IXG44_000566"
     CDS             9788..10057
                     /locus_tag="IXG44_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1657305.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            10219..10521
                     /locus_tag="IXG44_000567"
     CDS             10219..10521
                     /locus_tag="IXG44_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1657306.1"
                     /translation="MDKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVIGTAEQLASLTGLHEKIVRKRARCGYTDAKKRTFRYVEESS"
     gene            10518..10679
                     /locus_tag="IXG44_000568"
     CDS             10518..10679
                     /locus_tag="IXG44_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAQ1657307.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            10676..10981
                     /locus_tag="IXG44_000569"
     CDS             10676..10981
                     /locus_tag="IXG44_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1657308.1"
                     /translation="MSVDKKAAMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RRTPRKIAIWHDDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            10981..11337
                     /locus_tag="IXG44_000570"
     CDS             10981..11337
                     /locus_tag="IXG44_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HAQ1657309.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKTRSIQHGFI"
     gene            11297..11542
                     /locus_tag="IXG44_000571"
     CDS             11297..11542
                     /locus_tag="IXG44_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1657310.1"
                     /translation="MIKKLVQFSMDLYDIESGATLSVESDHLIINFGGKRQIILWVVD
                     DVLFPEIVHDFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            11539..11958
                     /locus_tag="IXG44_000572"
     CDS             11539..11958
                     /locus_tag="IXG44_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HAQ1657311.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVTGNIYENSELLEEQR"
     gene            11955..12512
                     /locus_tag="IXG44_000573"
     CDS             11955..12512
                     /locus_tag="IXG44_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HAQ1657312.1"
                     /translation="MNKQEAIKKLESIKAIGNDAIAACYNESINSGITLMKKIDEPQK
                     PVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLA
                     WATRQSYETLLSLYNGYEVEKEPLWAIKNADGNYLTKCALWGKDGVNYSFECNPSHRL
                     LFTDKATADAAALLVNGTVEEVVER"
     gene            12509..12805
                     /locus_tag="IXG44_000574"
     CDS             12509..12805
                     /locus_tag="IXG44_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1657313.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKSKFKVGDMVRCEA
                     EEFVYPFRGYVKKILSNSAIIRIENTMKCDRSTAKHKHYLAVARLVDMEVIKSK"
     gene            12882..13295
                     /locus_tag="IXG44_000575"
     CDS             12882..13295
                     /locus_tag="IXG44_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAQ1657314.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIETERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGKIIAYK"
     gene            complement(13753..14028)
                     /locus_tag="IXG44_000576"
     CDS             complement(13753..14028)
                     /locus_tag="IXG44_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1657315.1"
                     /translation="MYKPYMISYDLNNPGQKYDKVFEIIKEFGAYIKLQKSFWLVKTN
                     LNPNQMCEKLNTVLDNNDSLFICELQKNYQGRATEENWKFINEHIFS"
     gene            14482..14688
                     /locus_tag="IXG44_000577"
     CDS             14482..14688
                     /locus_tag="IXG44_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1657316.1"
                     /translation="MLGQCFGNHARFDSLPATLNAYDGYDYRKKIVKKLYGATYGNVV
                     SVLSVHHQASVTVAEVEDAAVNGE"
     gene            14884..15051
                     /locus_tag="IXG44_000578"
     CDS             14884..15051
                     /locus_tag="IXG44_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAQ1657317.1"
                     /translation="MYRPQYLEQKYEVITVHKGNGEKVYEYRRPIKSDTYKRKENNEV
                     IPLYGKRIAKH"
     gene            15077..15421
                     /locus_tag="IXG44_000579"
     CDS             15077..15421
                     /locus_tag="IXG44_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAQ1657318.1"
                     /translation="MTEEFYRWLLQLTREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            15426..15707
                     /locus_tag="IXG44_000580"
     CDS             15426..15707
                     /locus_tag="IXG44_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1657319.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            15810..16124
                     /locus_tag="IXG44_000581"
     CDS             15810..16124
                     /locus_tag="IXG44_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HAQ1657320.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            16102..17796
                     /locus_tag="IXG44_000582"
     CDS             16102..17796
                     /locus_tag="IXG44_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAQ1657321.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNADLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            17816..18994
                     /locus_tag="IXG44_000583"
     CDS             17816..18994
                     /locus_tag="IXG44_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAQ1657322.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            18957..19643
                     /locus_tag="IXG44_000584"
     CDS             18957..19643
                     /locus_tag="IXG44_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HAQ1657323.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            19643..20803
                     /locus_tag="IXG44_000585"
     CDS             19643..20803
                     /locus_tag="IXG44_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAQ1657324.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGGLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            20813..21688
                     /locus_tag="IXG44_000586"
     CDS             20813..21688
                     /locus_tag="IXG44_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1657325.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWAQLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            21685..21996
                     /locus_tag="IXG44_000587"
     CDS             21685..21996
                     /locus_tag="IXG44_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1657326.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            21986..22339
                     /locus_tag="IXG44_000588"
     CDS             21986..22339
                     /locus_tag="IXG44_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAQ1657327.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            22329..22730
                     /locus_tag="IXG44_000589"
     CDS             22329..22730
                     /locus_tag="IXG44_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1657328.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            22723..23127
                     /locus_tag="IXG44_000590"
     CDS             22723..23127
                     /locus_tag="IXG44_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1657329.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            23139..23747
                     /locus_tag="IXG44_000591"
     CDS             23139..23747
                     /locus_tag="IXG44_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAQ1657330.1"
                     /translation="MAKTKTVVTTFDNVSIKRIAFNFKNAGNAIATNCNGQLDGETEM
                     QTVVKKCGATEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     GEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            23767..24129
                     /locus_tag="IXG44_000592"
     CDS             23767..24129
                     /locus_tag="IXG44_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1657331.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            24132..24314
                     /locus_tag="IXG44_000593"
     CDS             24132..24314
                     /locus_tag="IXG44_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1657332.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            24331..27762
                     /locus_tag="IXG44_000594"
     CDS             24331..27762
                     /locus_tag="IXG44_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAQ1657333.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVKKLERELIN
                     QQTAQQRLSNEIDKTSNALAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     ATAGQSASEAEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLSKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDFIVAIGGLTAAFGILLPAILAVSYLFGPMML
                     IIGGIIAVIAGVIVAIKNWGAITDWFSGLWKKFTDWLGDTWESIKDGASSVWDGVKET
                     WSGFVDWVQDIWQGVSDWFGELWSGLVEGASNIWQGVQETWQTFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNGIWTAISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWNSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISGIFDNLNISIPHIPLPEFIMEGSF
                     NPLKGQIPHLRVKWHAKGGIFTKPTLLGGMNGVGEAGPEAVLPLKRSVLQEIGDRILS
                     STSVSSRAQTIQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKIQNDNASAWR"
     gene            27813..28550
                     /locus_tag="IXG44_000595"
     CDS             27813..28550
                     /locus_tag="IXG44_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAQ1657334.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            28560..30851
                     /locus_tag="IXG44_000596"
     CDS             28560..30851
                     /locus_tag="IXG44_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1657335.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYSINGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKVLAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHEKSLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLLNDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGADYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDNGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            30875..33001
                     /locus_tag="IXG44_000597"
     CDS             30875..33001
                     /locus_tag="IXG44_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1657336.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDNVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNETFDAFMAEIKGRVDSLNQQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSIDFGDYDYSENPNLMPYITEPWVG
                     PLLGNGHTVKDSVKRVITHTKTRTANSGDILSLGLGIPCTAEANNRYLITTLRPSTTY
                     TLSVTMSVGSDWTGETNTIGVRLRYLNEQGGIELPINALIPANVERDKMVTHTFTGIT
                     KDNVTSITNCYVEIFSLNSEYKGTVSVSYDVKLKAHYPNLLDGPYWLGKVPLGENIAD
                     PTVVFPHKTSEYMVYGRRNTENYIADQTYTISMKATKLTVQSFAVYIAAGRVKVGDMK
                     PTEGLANTWELTFTVTKQHIDSGVTNYLEIYQYPSATKGAVQIEWLKLEKGNTRTPNI
                     SEYKYRGTGMRDSNNPKDYVWDLAPEYVEDNLATDIKISEITGKANNYTDGKVSEINS
                     QLTASINEVDTTAKDAQTKANANATAIDELDNKIDERINDTATTTLTVTNGNTGSAKL
                     YREGKTVSIYFVALNGKSSGGNDSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSI
                     GADGAIKWRRNSSYGSDYTFAITYTI"
     gene            33164..33610
                     /locus_tag="IXG44_000598"
     CDS             33164..33610
                     /locus_tag="IXG44_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1657337.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            33612..33749
                     /locus_tag="IXG44_000599"
     CDS             33612..33749
                     /locus_tag="IXG44_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAQ1657338.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEEKADKIISKEESA
                     S"
     gene            33787..34080
                     /locus_tag="IXG44_000600"
     CDS             33787..34080
                     /locus_tag="IXG44_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1657339.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKTLFRERRK"
     gene            34077..>34296
                     /locus_tag="IXG44_000601"
     CDS             34077..>34296
                     /locus_tag="IXG44_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAQ1657340.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGN"
BASE COUNT        12032 a         5248 c         6941 g        10075 t
ORIGIN      
        1 gtatgcctta gtccatgcaa agtaaattgc ttttcaatcc ctaattcttt taattttttg
       61 cgcaaatatt tgctgacggc gtttgatgaa actaatccat tttttaagtt gaaaaaaaca
      121 aaattatgag ggtttttgat ttcaaaattc tcgaataatt ctttttgatt gattttaaac
      181 ttttttaaga gatcaatcgt atgtttatca atggaaattt tcctatttga agtttcgttt
      241 tttgtttttc cccattcatt taatttataa ttccatgttt tattaatagt gatgatttga
      301 tcttcgaaat cgatatcttc ccatgttaat cctagaagtt cagcaaatcg cattccagtg
      361 gcaccagcaa ctaacacgag cataggagaa gagtactgcg cagaaaggtt ttcttctacg
      421 agtttcatta aagatttaaa ttgatcataa tctaaatatt tatcttcttt tggcttcaat
      481 gaatcttttc ctttgattac tgcttttcta gttggatcaa acggaattaa tccttcttct
      541 acggcatctt tcaacgacgc tctaatatgg ttattaaact taataacggt agattttaca
      601 tggtctttag catacttatt taaaaagcgt tgatatccca ctctatctaa atcagaaatc
      661 aataccgctg gcatatattt ctttatgttc attaacgtat cttcgtattt tctataagtg
      721 ataggagata ctgttccttc tttataaagt tgcatccaat cctcaaagta atctgataat
      781 aatagatttt tgcgatcccc tttaaggcct ttgttcaagt tatattctaa ttcgttggcg
      841 gcatcttttg cttctccttt tgtcttaaat cctgattttc ttatcttcgt gtattttccg
      901 tcatcttttt tgtatgaaat ttcgtattgc caactatttc cacgtttgac taatcttgcc
      961 ataattgaat cagctctctt tctctgatac aataggaact ataaagaagc ccattgtttg
     1021 ggttttatta ttttttgcac atcctttttg cttgccggcg caaggatgtg ctatttgtcg
     1081 ttttaatggt ataattggat gtgtattaga tcatccaatc agaggtgttt attatgatta
     1141 aagtgatttt taaaagaata actgaacttc cagagtttag attatttgtt gattattggt
     1201 atgtctggtt ttttattgtt gcattgctga taggtctaga tgtcatatta gaaagaaaac
     1261 ttaagtaaga gttgagttaa taaattgaat tcctaaatga gatattttgt agcccatatt
     1321 ttgtttgccg acatcaccta atactatgga gctaatcgtt ttttctaaca gaccaaaatc
     1381 agataatcta tttaagctac catctattag ctcttgtgaa tagccagaca tgctgatatt
     1441 tccagtgaaa ttttcgtttc cacgactcat taagcatgct aacaattgaa tctcaatcga
     1501 tgttaattga ttcagtacaa gaagataata ctcctcgtta tcccatgaag cgttgttaat
     1561 tcgaagtaaa caattcttat aaagattttt atacatatct attttatttt gtgcctttga
     1621 tttttctacc tcgtcattta tcccttcgaa tattccaatg agttggtcct tgttctctaa
     1681 gttgaaaaca tcttctggaa tttgatcgcg aatgttttct aatgattcgt ttaaattttt
     1741 gtagaatttc tcgatgcgtt tgaaacgctt ctcgttttta gacccaaaat aaagtgactg
     1801 cacaacgcca ccaatataag gaattgctcc aataccgcct tcaactatta attcaagata
     1861 ttcttttttg gttagttttt cattgctatt atccatagtt acctcctatt ttgtcatttc
     1921 ctttacaagc attgtgactc ctgatggcac tcttttgtat aggttttatt attgtgcaca
     1981 atctaactct tagtggggcg tggttcattt attttttact tactttacta ttaatcatat
     2041 cccattgagc ataggatttt tctatgttcg atcctcttga actaatttct cctgtaaaag
     2101 ttactttatc gccctttgta aaggtatcag cattgtttaa gtccttcgca aaaattacat
     2161 atgaaacttt aggtgttgca gaaatatcat tccaactttt atcatcgtac tctttgtctg
     2221 ctataatggc tattctagta gtcattgatt cgataacagt tccttcaaaa gtatatgttt
     2281 taccactaat atatttagta aaagctgtat tttgctcagt agctggagta atagaataat
     2341 aactatctaa gaaagcaccc cagtcatgat tatttgtctc taaaaattct tcaaattgag
     2401 ttaggctttt attgctgctg ttagatgtag tagtttgagt atcagatgat gagctttcct
     2461 gtttgttatt gtcatttgcc tcgtttgatg aggaaagcga gaaacctacg gtagtaaaaa
     2521 taatcccgat aataaggaga atcataggtg ttttcttatt tgtttttttg ataaatctta
     2581 caattaacat gattaaacct ataataaata aaacaatacc agtaaaacca aatataccgt
     2641 ccattaatag atcctctttc tcttttgata ttgaatatta taaaccaagc aattcttttt
     2701 tcttaaaatc gaattcttct tgattaataa tcccttcatc taacaactct ttatactttt
     2761 taatttcatc tgcacttgaa atttgtgttt ctttggaatt attatctaat atagattgtt
     2821 gcttctctat atattttttt aaattttcca tttcattcca atattttttt gtaaacataa
     2881 cggtgttttc atctttcgtg gccgctaata tacctccgcg actttcgttt ccgcctagca
     2941 aggtaaattg tatataacca ttactcatac ctggtttttt taattgtacg gctgatatat
     3001 ttttaaaggg gatagttttt tctcctttaa taccttgatt aactaaattt ataaatcctt
     3061 ttctaataat tgaaatagaa tcatcagtta ctttaatcaa ggtttttcct ggagatttta
     3121 ttaaaatctc tttttccatt ttttcttcct catttctgtg atatgatttt ttttgtagaa
     3181 gctcatatat gaggaaagag cccgtgttgc agcacgggct tttttaatgt tttgctgaaa
     3241 tcggtttttt taataattct tgatgtattc atacatattg ccttgcgtaa gaatattttt
     3301 tcttaaaata gcattggcag acagcataac aagtgtatct gagcttattc tgaatagaat
     3361 aatactccat aaatttttcg agattaaact gagatttatc agtcagttcg ttctcaatat
     3421 agatatttaa taaaattaga atagctattt tatctgcttc tgtttcaaat tttgagtgaa
     3481 aagttgtaga agtatcgtac aaaactgaaa attcaaaatg agaggcgctg aaatgtgcaa
     3541 gttcatgaga taaatgaaag gcttctgcag tttcaccgta tagattttca ttcaaaaaaa
     3601 tgattctggg ttttggatag tagaaaccag gctctttcat ctccatataa actactttca
     3661 aattgtattc gctcaacatt tctttcaatt tcaaatacat acaaaccatc actccaacta
     3721 ttcattttcc tctaaagctt tagcaattgc aatcgcttta cgcattgtct ccttagatat
     3781 ttcttttcca tcaaaagaaa aaacagtatc gtcttctgat aaatccacat gtttaggggt
     3841 ttctctttct tctctaccta gaaggtagtc tacagagaca tcgaaatagt cagcaatccg
     3901 acttaattca tctgaattag gagtattatt tttccacttt gctagataac cgttagaata
     3961 tccaaaatta atttctaact gccgtataga tatctttttc ctcttcgcca agtcttttat
     4021 tatttcatag gtattcatcg ataaaacaac ctttctaaat gtttacaaaa aaagtttaga
     4081 aaaatacgca gaaataattt gactaattct gagtaatgag ctatactata tcttgtaaac
     4141 agatttaaca actaaaaaga caacaaaaaa cactattgat atgtaaatgc agaccgccaa
     4201 gaaagcttta aaatcaatgt ttttatgtct tatttaatta tgctcttagt atagagtatt
     4261 actcagatac tgtcaattga atttagaaaa aagttgttaa atttgtttac gaatataaaa
     4321 gaaaggaatg agaaatatga acacaccgca aatttttaat ttcgaacaaa acgaagttcg
     4381 gacaatttta gtaaatgatg aaccatattt tgtaggcaaa gacgttgcga gtgttttggg
     4441 ttattcaaac actaaagatg ctttgtcgcg gcatgtagat ttggaagata agatggggtc
     4501 gcgaatcacg acctcaggtc aatcaagaga gatgacaatc atcaacgaat ctggtttgta
     4561 cagtttaatc ttaaaatcaa aacttccctc tgccaaaaaa tttaaacgtt gggtaacaag
     4621 tgaagtgttg ccagcaatta gaaaacatgg aggttatcta actccagaaa aagtagaaga
     4681 agctttgctt aatccagata caatcattca attagcaact caactaaaag aagaaagaac
     4741 tggaagatta atcgcagaac aaaagattgc agagtacgaa cctaaaatct cctatttaga
     4801 tagcatatta tcttctacag attcagtaac aattagtcag attgcagcag attatgggat
     4861 gtctccacaa cagatgaata aattacttca taaactaggt attcagaaaa aagtcggtaa
     4921 ccaatggtta ttgtgcaaaa aacacatgaa ccaaggatac acaaaatctc atacaactga
     4981 gatcccgaaa gccgatggtg gcactaaaat tgtaatgaat accaaatgga cacagaaagg
     5041 gcgtctattt atctacgaat tactaaaaaa agaaggatat taccctcaaa tggatttaga
     5101 ggaaattggt tagaaaggag ttttagtatg actgacattg cagaaatcac tcaacgagat
     5161 agagaaaaaa tcaaagaata tgtcgaaagt tcaaagttct taacttacac catgcttgct
     5221 gaaagatttg gaattagcaa aagctactta tctttaattt taaacggtaa aaagacttct
     5281 gcagaagcaa acagaattat agattcgatt atcactatgt acgaattgta aagggaggaa
     5341 cagctaatgc aatatctaga agcaaaaatc ccaattccag aaggctatgt aattatctcc
     5401 caagtggatt atgaggagtt aaaaaaagct gatgatactg gtagatggat gacgttgcca
     5461 gaagtgctag aacggattaa cagaaaatat gattggttta cttctagagt tttaaagaac
     5521 ccaagatata gaaatattat cgatatcgag aaaaacaaaa atggatttgt ctattatcca
     5581 gttgaaggaa gagacacata tctattttta agaagtaaaa cacttgaatt tttagaaaca
     5641 aatttttcgg aaatcttaag gaggcaagcg gatgggaaaa tttaatagag cgctagtgtt
     5701 cagcgcaccg ctaatcatct acgctttagg actttgggga agcaggcaag cgttgatagg
     5761 aacgattgtt tacatggttt ggatttttat ggggcttgat gaagctgaag ctgagtacag
     5821 agcgaaaaag ccaaccgagg gggctgacta aaaatgaata gaaaagagaa actagaatgt
     5881 atattactat tactcagttt aattctttca ctaatttctc tattgggaag tttgtatttt
     5941 tgatatcaac aattttgtgg atttgggttc tatttagagt aaatatcatt tttgtttggt
     6001 gattaaggag tttattaaaa ttaattttcc ctacttcaaa tgcaaggtaa caagatatag
     6061 cacttttagg aggaatattt agcggtacag cttcagagta tattctatta tcatcgactg
     6121 atcctccttt agcaattaat ttcttaaatg gagtagctga ccatctgcgg tttaagttag
     6181 cggatgaaag ttctaaatta actaacatag ctggttctgt tgaatagtta gaaatgatta
     6241 cttttgtata aatcatatct gcagcgaagt aagaagcatt aagttcaact tctagttgag
     6301 ggcgcattcc ttttattttt atccctgtta aaaacgtact caatataaaa cttattattg
     6361 acatccattg aaaaattgtt aagtgaaaaa aattcaaaat aaacaccacc agtttttaac
     6421 taaattatac caaaaaggag agaagaaata atgcaagaat tagtaatttt gaaaaataaa
     6481 gaagctgtga ctacgagctt acaagtggca gaaagcttcg agaaaaaaca tcaacatgtt
     6541 ttaagagata ttgatgcact aaaaaaagat gtgtccaatt ttggacagat gtttgtagaa
     6601 ggtaatgaac cagattcata tggcagaaat cgacgagttt tcttcattag tagagatgga
     6661 tttttcttgc tggctatggg gtttacagga aagaaagcta tctacttcaa acaaaaatac
     6721 attgaagcat tcaacgaaat ggaagatgtt attcgcaaga atactgttcc tcaaacaatt
     6781 gaagatatga tgatctatca attagaagaa atgaaagatg ttaaaaaaga tgtttccatg
     6841 cttaaagata ctatgcgaat tagcggacaa caagagtttg aaattaagca aaaaggaaat
     6901 atgaaagtta tgggagttct agggggaaaa gaaagccgag cttatgaaga aatcagcaaa
     6961 aaagtattct caaaattttg gtctgaattt aaacgtacct tttcaatccc aagatatggc
     7021 gagttacctc gtaagagatt cgatgatgct gtttcattta ttgaaatgtg gttgccagaa
     7081 actgcgatcc gtatggaaat tgatcaactg aacagacaac agagactttt tggtgatgac
     7141 aatgaataga gctgaagcgc taagaatagg gacggcaatt gctaattgct ggtggaaata
     7201 ctataaacca atcatcctaa gccaacaaca tattgacaag caaaaagcat ggcaacaaat
     7261 aaaaaagtga ctccgccggc aagcaaagag tcacaaagaa aacacatcat aaggagattt
     7321 tagcatatgg aaaaagaact ttccactcta gatcaatatt tgattgatcc tgattggggc
     7381 aagccgaaaa ttgaggaaac aagtggtcga aaaatcagac gaaatctttt gacgaatgaa
     7441 gaactagctt gggatcaaga tgatttaggc aaccatgtaa ctatttggga tcatgtttat
     7501 cttatccatc tatcgaagca ttcgaataaa cctgaatata tttacgtcat cgaagatggc
     7561 ttgattgatg cgctagaaga gtacgacaga gataacttga ttgatatctc ttattacgga
     7621 ccaggtaaga aatacattgc tgaaatggag gcagaatttg atgagtgaaa tcaaagggac
     7681 aacgaacttt gaaaaacttt ttagtcgtaa gttaaataaa attctcaaga aaaaaggaaa
     7741 ttttgattat ttatcttggg ctcacgcgtg ggagattatg aaaaagaatg atccacaggc
     7801 aacggtaact attaatgagt acaaacacta cagggttgtt tctggaactc atcaagactt
     7861 tcttgttgag gaatataaac cttttcttat ggatgaaact gggacttatg tatctgtctc
     7921 agtaacggtt aaaggacaca cggaaaccga attatttcct gttttagatt atcgaaacca
     7981 accagttgtt aaaccaaatg caatgcaaat caataactca ttgaagcgat gctttgtgaa
     8041 agcattggct ctacacggac tgggattata tgtatttcaa ggggaagata ttccaacacc
     8101 acctagaatc gatacaaaga aattaaacat gctagagacg attctagaag ctttcaatga
     8161 gcagatgggt aaagatatga ccaaaacctt aatcgaatat gttaatgagc agacagataa
     8221 attagggctc ttagctgata acgttgaaac tattgaacag ttaagctatg agcaatgtgc
     8281 cttgatggag cgagcaatag cagctaagag aaaagaatta gataagaagt gatatgagtg
     8341 tttaaaccat taatcgattc atattcagcg gttctgaaaa agttcaaagg aaaagacata
     8401 agcgcaacca tcaatgagga agtgaacatt gatcgactaa agacgatgta tgacggctac
     8461 gatggtgatc gagtcattga aattcgtttt attgatccta gacgtttcac cgtacagcaa
     8521 cgaaacttca tctatgcgct gataggcgat atttttatcg atacaggcat gccaacggac
     8581 ttctggaagg aattcttcta ctttcgtttt gaaggtgtca cagggcgcaa aataagcctg
     8641 aaagacgaat cgaatacaac tgtgagtgat gccaatgtct tagcaaatat catcttagat
     8701 ttcatctttg aacatcatat tcctttcaaa gaaggctatg agattttacc tgcgaatcaa
     8761 gaatattact tctacaaatg catcacaaaa agagtctgct gcatctgtgg caaaacagga
     8821 gctgacatcg atcactttga caaagcgcta ggaagacgaa agcgcaaaga agttgatcat
     8881 gcagagtaca catttgcagc actctgcaga atccatcaca cagagaagca caaaataggt
     8941 gtgattaatt tcaaaaataa atatcaaatc aaagggatca aattaaacca gaaaacaatc
     9001 aaaaagttaa atattggagg gtaaaaatga cagaacatcg aagttattat gcgattatac
     9061 cagccaacgt aaggtacgac aaaagactta aaccaaatac taagttgtta tacggagaga
     9121 taacggcctt gtgtaatgaa agaggctttt gttgggcagg caatgagtac tttgcagatt
     9181 tatatggtgt gaataaagag accatatcgc gatgggtaag tgatttgatt aagtttggat
     9241 acttgaatcg ggaaatcatt tacaaagagg gtaccaatca aataatcaat aggtacctac
     9301 gaattaatca ataccctatt gacgaaaaac gcaatacccc tattgacgaa aaagtcaaag
     9361 ataataatac atctattaat aatacattta ataatacaaa agaatatata agagagttac
     9421 cgccttcgaa aaaatcgaag gctaagcccg tccgtcataa atacggagag tataaaaatg
     9481 ttcttttgtc agatgagcag atggagaaac tcaaaacaga attccctaat gattaccaag
     9541 agcgaatcga acgactgtca gagtattgtg aatcatctgg taagacttat aaaaactatt
     9601 tggcaactat tcgaagttgg gcaaggaaag aaaaaagtga acctaagaac gcaagcagtg
     9661 gatacaagcg cacaggaaga cgagagaagc ttcctgaatg ggcaatcgac caagaagcct
     9721 atctcaagaa aaaagcgcta gaacgagcta atagacaatc aaaagcacca ttctaagagg
     9781 tggaaaaatg aagatcgatt atctagaact aattaatgaa atagcgaatt ataaaaaggg
     9841 cgaggaatta gacgtcctga gagacgtata tgatcaactc gaagaagctg gaattgaagg
     9901 aattaagaat gatcgttcga gttggagtaa gctcagatac tatttcgcac tttatatcga
     9961 tacaacacaa ttaagaaatt tagcatatac caaattacta tttgttgatt gtgttaaagg
    10021 cttgcaaaaa catcttaatg aacttgagca ggtgtaatca gatggaccta aagacattta
    10081 cagcacagat tgaactaatg catcaagaag ctttaagaca aagtgtatcg tacgaagaca
    10141 agtggctcaa cacgttccat ggcggacgtg agagcgcact tgatcaagta ctcaaattac
    10201 tgaaaggaga atgtcgggat ggataagaaa gcggcaatgc agcgaattat cgaattgact
    10261 tattcagaag attggcaaaa tgacaaagaa gctgcttcag aagtgatgag gcttggaaga
    10321 gcgatgtggg cagacaagag caacaagcca agaccacgaa aaatcgcaat ttggcacggt
    10381 gacaaacttc tagtgatagg gacagctgaa cagttagcaa gtctcacagg cttgcacgag
    10441 aaaatcgtga ggaaaagagc aaggtgtggc tacacagacg ctaagaagag aacgtttaga
    10501 tacgtggagg aatcatcatg acaacagaag aagtgattca aatgcgtatt cgaagccttc
    10561 agcgtgagat tgacgatctg gaacgaacaa aggcagtgat ggtcaatgaa acggcgagaa
    10621 aggcaatcga tttgcacata gagaatttaa gaagggaaat ccatcgattg gaggaatgag
    10681 cgtggataag aaagcagcaa tgaaaagaat tgctgaatta accaagtcag aatcttggca
    10741 agaagacaaa gaaatagttg cagaagtcca aaagctcggt aaatcaatgt ggactgaaaa
    10801 gcccaaacgg agaacgccga gaaaaattgc aatctggcat gatgaccgaa ttttagtaac
    10861 aggtactgct gaacagttat ctgaaattac tggattaagc aaaaacatta tctgggatag
    10921 agctaggagc ttatggattg attcaaaagg acgacagttt aggtatgtgg aggagagata
    10981 atggatctca ttacacaata cagtgacatc atcctcaaga aaatcatgat gaagattcag
    11041 aaagataaaa aatcaaaaga acgagctgaa ttagttaagt tagaaatggc tgaaacagga
    11101 gcaggagtgc gaagtagcag gcattggaaa gcagcagcaa acattgaatt ttattacaac
    11161 gaaattcaaa aagggttcga tcagatgcgt gagctggatc ggcaaacaaa ttggagcaag
    11221 aaacttcatc aagatcgttt caaatttgta gagaagtata aagaaatatt agaagagtat
    11281 ttgaggagga cagcaaatga taaaaaaact cgttcaattc agcatggatt tatatgatat
    11341 cgaatcagga gcaacactat ctgtggaatc ggaccatcta atcataaatt ttggtggaaa
    11401 gcgccagatt attttgtggg tagttgatga tgtactgttt ccagaaattg ttcatgattt
    11461 cgaagaatca aaagcggttg agtttgaaat agtgaaaaaa gtaatggaat tgattgaaaa
    11521 atacgaggag gacagcgaat gataccgaag tttagagcgt gggataaacg aaagaacgta
    11581 atgagagatg tagccgtctt gcattttact aaaaacggca aaacaaactt tattgaatat
    11641 tggataaatc ctaccgaatt gaaatcatat cacgtgcgaa acatcgacct catgcaatcc
    11701 acaggaatga aagataagaa tggtgtggat atatttgaag gggatatagt attagtcagc
    11761 gtgcgaaatg gcttcgatta cttagataat aaagtctgta ttgtcaaaaa ttcaatagat
    11821 tattccggat tagtttgtgc cactgttgat gaagacttag agtatcaaat ttttaacaca
    11881 gagctgtttg aagaatacac gtacgaagtc accggaaata tatacgagaa tagcgagtta
    11941 ttggaggaac agcgatgaat aaacaggaag caatcaaaaa gttagaaagt attaaagcga
    12001 taggaaatga tgcaatagct gcttgctata acgagagtat aaattcaggt attacgttaa
    12061 tgaaaaaaat agacgaaccg cagaaaccag ttattccaca actcgtggcc ggttggcttg
    12121 agaaatctac ggaccctttt acaaaagctg aaaaaatagc gtatttaatc aaatctaaag
    12181 atggtgattc atattatttc tgtgattggt ttgtacgaga tggcatagtg acgcaagagc
    12241 aaggagaaga attactcgct tgggcaacaa gacaatcata tgaaacacta ttgagcctat
    12301 acaacggcta cgaggttgag aaagagccgt tatgggcaat aaagaatgcc gatggaaact
    12361 atcttactaa atgtgcttta tggggaaaag atggagtaaa ttatagtttt gaatgcaatc
    12421 catctcatcg attgcttttc actgataaag caacagcgga tgctgcagca ttgttggtga
    12481 atggaacagt ggaagaggtg gtagaaagat gaaactaaaa gacggatttt acgctagcag
    12541 tcacggtatc ggcggtttaa tgctagatat gccgacaaag aaccctaaaa cacgcaagaa
    12601 atcaaaattc aaagtcggtg acatggtccg ctgtgaagca gaagagttcg tttatccgtt
    12661 cagaggatac gttaaaaaga tactgtcaaa ctcagcaatc attcgtattg aaaatacaat
    12721 gaagtgtgat cgttcgactg caaaacacaa gcattattta gctgtagcga gattagttga
    12781 catggaagta atcaagagca aataaaaaaa gccggatcgc tccgactgat tcaataaatc
    12841 caacacattt attatatcac ataaaggagc ggtttgactt gatgcaattg ttacgagagg
    12901 tagatttcaa acagacaaga tgtaatgcga gagatgtgct gaagaacttt cggcgtttgg
    12961 agcggatggc aggtcgctct ttgatagata ttaagtcgcc gattataacg gatatgccga
    13021 aggcaccgaa gcatggcaat aaggcagaag acgcgattat tcagatgatg gatatagaaa
    13081 cggagagaga tgcgattcta gcggctttga tggctcttag tctgattagt cgtcagatac
    13141 tctactacag cttctgtgtg ccagatagct tctcaaacta cagaattagc cgtgaagtgg
    13201 gttattcaga aagaagtata caacggatga agtcggaagc tctaatagag tttgcagaag
    13261 catataaaca cggaaaaata attgcttata aataatttgg cggttttttg gcggaatgat
    13321 ggcggttttt agacatttac cagtgttatt atggtagtgt cgaaagatta ggaaacagga
    13381 tcgacaaaat aaacgaaggg aggcaatctc cctcatcgtt ttaaacttct tcttagatag
    13441 acagcaacta attatgaaga aaaggatgtg aattcaactc ctcctaaatt cttcatcggt
    13501 tgctgtttat tttgattaga cagcagcact cacaaaacta aacatgttct tgtcgtcttt
    13561 caactgctgc tgtctattaa tttatgtatt ggagggaaga aaaatgaaat tatcagtaga
    13621 aggcactcca gaagaaatac aagaattgct ccaagctatt ggtagtagca aggagcaaaa
    13681 ggtaagtgtt tctaattcag atattgacgg tatttgcaat tgtttaaaca gagaaaaaat
    13741 atctaatcct tactaagaga aaatatgttc gttaatgaat ttccaatttt cctctgtagc
    13801 tctaccttga taattttttt gcagttcaca gatgaataag gaatcgttat tatcaagtac
    13861 tgtatttaat ttttcacaca tttgatttgg attcaaatta gttttaacta accaaaaaga
    13921 tttttgtagc tttatatatg ccccaaattc ttttattatt tcaaatactt tatcatactt
    13981 ttgccctggg ttattaagat cataactaat catgtagggt ttatacatgt tgtcttgcac
    14041 atccttgttt tatatttcag cggaccactc gctgataaat aaaattatac gcttagtatt
    14101 tattttcaca atattaattt atcgtaataa cttttgagta aatagaacaa aaaacctgca
    14161 ctagtttccg ctagtacagg cagtgactat atcggttgat aatctagcat atattaaaaa
    14221 taattgcaag aaaagaaatt tatagtggtt tataccaaat tatcaaagca atgttatttt
    14281 gttgctgtct attgtttttt aattattcac acgataacta aaggtgggtg aagagaaatg
    14341 attccattaa taatttcaat ttttgcgctc tgtcttaatg tctatatgat tggatttaaa
    14401 aatgggcaaa ataaaaaata gtagcagcca agaataattt tatagtgtca ctgtggcgga
    14461 aagggtagac gctaagcatg tgtgctaggt caatgcttcg gcaaccatgc aaggttcgat
    14521 tccttgccag cgacattaaa tgcctatgac ggttacgact accgaaaaaa gatcgttaag
    14581 aagctatacg gtgctacgta cggcaatgta gtaagtgtgc tatctgtaca ccaccaagct
    14641 tcggtcactg tggcggaagt agaagacgca gcggtaaatg gcgagtagcc tcgtgagagc
    14701 ctggtaagtt ctcgtgagtg gtgcaatccc actccagcga ctttaagcaa ccgaggcatc
    14761 ggcggtttaa aaatataggg gtgcgcaatt tcgtacgcgt tttgtgcatc gtgcaagtta
    14821 ctattacata ttagatcact ctttgagtgg tctttttatt tttgcacaaa ggaggaaaca
    14881 acaatgtata gaccacaata cttagaacag aagtatgaag taatcactgt tcataaaggt
    14941 aatggcgaaa aagtatatga gtatagaaga ccaataaaga gcgatacata taaacgaaag
    15001 gaaaacaatg aagttattcc attgtatggc aaaagaatag ctaagcatta aataagattg
    15061 cgaaaggaga cggaacatga ccgaggaatt ctatagatgg ctattacagt tgacaagaga
    15121 agatcgtttg gttaagttct atcagtctcc taaatggcgc aggcttagag agaaagcgat
    15181 gaaacgagat cactatgaat gccaagagtg tagaagacta ggtaagtatc atagagtaga
    15241 gaacgttcat catataaagg aagtcaagga tagacctgac ttagctttag atttagataa
    15301 tcttatttgt ttatgtgttg aacatcataa tgaagttcat ggcagatatc ttacagcatt
    15361 agataaacaa gagaagaaga tagaaagctt tgctaacttc gatgcaagtg aaaggtggta
    15421 agtgcatgat catcaatgat aatggcagag agtatgatac agaaaagatt gaagagtatt
    15481 catcttatac tcagggatta attaaacgtt tgatatacgt tcgctatgta ggtattaggg
    15541 atctgttatc agataactgt tgtagtaaat acaaagtgaa tcaagtaaga gaagcgttga
    15601 ataaagataa taacgtcgaa agaataaaaa atgtttttgg atatagtatt gaagagatta
    15661 attattacat tgacttcgct gaagctttca ttccgatggt gagataaccc ccccttaaaa
    15721 taaatcgcaa attttttggg ggtgatgaaa cggagggggc tgtcaggaaa agagattttt
    15781 tcgaacttta tcatgaaagg agggctaaaa tgtttaaaaa cgaattgtct caaaatcgct
    15841 acagagaaaa attacgccgc tctttaataa gccaattgga aagtcagaaa acaaatattg
    15901 agccattctt agataatgtt gatcgttata tcagtttatg ggaaacggcg atatcactgg
    15961 aagaagatat atccgagaac ggcattagac tggagaatgg taaaaagaat gaatcagtag
    16021 cgttgcttgt ttctgtcaac aaacaaatgg gattgatgtt ggataaactt gccattactc
    16081 ctgaattggt aggtgaagca aatgaatcaa ttcctgagtt ataagcatat tgaaaattgg
    16141 ttcaaagcta tagaagaagg cactatcaag gtatgcaaag agcaattatt gctaaaaaat
    16201 tatctagaag aaagagtctt tactagagaa gatatttact tcgataagca gatggtagag
    16261 gattcaatca atataccagc acaatacttt ccattcgaat taattccgtg ggaaaaattt
    16321 ctacaatgtt ttatttatgg tgttcgatgg aaaaaagata aaacactagt gttcaataga
    16381 tatctttcat taatgggacg tggtaatggt aaaactggtt ttgcttcttg gaacaacttc
    16441 tttctactaa ccgctaaaca cggtattaaa aattatgata ttgatatcta tgccaataat
    16501 gaaagccaag caaagactag ttttgatgat gtatttaaag taattaaaga tcatcctgat
    16561 ttagataaaa aagtatttaa agctacgaag gaagttattc aaaatatcgc tacaaacagc
    16621 aaacttcgtt ataacacggc aaatgctaga acaaaagatg ggaagcgacc aggtgcaaac
    16681 cgctttgatg aaattcacga aaatgaagat tattcaatga taaatgtggc tacttctggt
    16741 ggtggtaaaa ttcgagatta tagagaattt tatgatacaa ctaatggtca tgttcgtggt
    16801 ggtccgcttg atgacattat agaagaatca aaaatgattc tttctggaga acttggaatt
    16861 gacaaggatg gagcagaatt ttctagtttg tttccattta tttgtcgctt ggataacgat
    16921 aatgaagttg atgatcccga catgtgggaa aaagcttgtc caactattaa ttacaatgca
    16981 gatttaaaac ggaaaatgtt tcaagaatac tctcaaatgc aacgtaatgc tggtttaaga
    17041 cttacgttca tgaccaaacg aatgaacaga cctatggaag atacacgatt tgctgttgct
    17101 tcatatgatg atgttctgca tacgaaagaa aaagaatttc ctgaaaaaat ggatgaagtg
    17161 ataggaacag tcgattttgc tgatagacga gattttgcca gcgttgggtt gctaggaaaa
    17221 tacgataaag atgtttattt tacacaacat acttttatcc acgaatcagc ccttcgatta
    17281 caaaacatca aacgagaggt tatagatatt tctatagatc aaggaaaatc acagatcgtt
    17341 catggaaaaa atatagaagc tgattatatt gtaggttggt ttcttgaaat gagtaataaa
    17401 tattatatta aaaaaatcgc tatggatatg taccgtgcaa aaatattgaa gcccgcttta
    17461 gaagaagcag gttttactgt ggaaattgtt cgaagcggat ctgttacaca tggtatgtta
    17521 aaagatctgg ttgatgacct ttttattaat caacgtttat tttttggtga cgatgcgatt
    17581 atgcgttggt attgcatgaa tgtatatgaa gagcatattt ctaatggaaa tatacgctat
    17641 gaaaaaatag aacctgaaac tagaaaaacg gatggctttt tttcattcct tcatggtttg
    17701 aattttttag atgatattta tgattctgct cctgtaacag tcacaaatag ctcagtagaa
    17761 aatacaggaa ctggatttac tcctctagta ttctaacttg aaaggaggtg agaaagtggg
    17821 gatttttcaa aaggcggtag gatacttcac aaaaaaagca acggttcctt tagaagaata
    17881 cttttgtaaa ttgcaagttg attttgtgta tcgaaaattt gcgattgaaa cttgtattga
    17941 tttgattgca aatgcgatga gtaaagcgga attcaagtca tatgaagatg gaaaaaataa
    18001 aaagaatgat ctttactata ggctgaatgt agctcctaat aagaaaaata atgcaacaga
    18061 atttagaaaa aaactgatca ggagattaat attctacaat gaagtattga tcgtttctcc
    18121 gtctaataat tctagcgaaa tatttattgc ggatagttgg gatgtcacag aatatgcatt
    18181 gaaagatgat gtgttttctc aagtgcaaat taacaacata gtccttgata gagaatttct
    18241 agaaagtgat gttatctata taaaatacgc agatcaacaa attaggcaac tagtcgatgc
    18301 gtattatcaa gcgtatggga aactcatttc tagtgccatg aatgtttaca agcgctctaa
    18361 cgctcgtaga tacgtactga aagggaattt attccgaccg caagacaata caacacaaga
    18421 tcaaatcaat aaaatgatga catcacaatt taaggctttt atggaagctg ataatgcagg
    18481 tgcggtattt caattacaaa atgagtacac attagaagat ttcagcggaa acttccaaag
    18541 caattcaaga gatataaaaa acttaataga cgacatcttt gagatgacag cagcagcgtt
    18601 tcacgttccg aaaaacctac taaagggaga catgagtggg ttatcggatc aagtggacgc
    18661 ttttttaatg ttcgaaatca taccgattgc tgaacttatt caggatgcgt ttaacgctag
    18721 tctctatgaa gtagaagaat acttgtcagg gaattttgta cgtgtggata caactatgat
    18781 caagattact agcttcaaag atttggttga cgctattgat gtaggcatta gaaatggagt
    18841 atttacaatc aacgaaggaa gagaacgcgt tggaaatgat cgctctgata aggcgatggc
    18901 agatgaaata tttataacta aaaacaatca acaagtatcg aaaggaggtg aggcgaatga
    18961 cgacaatgaa aacatttcta gcagtaaaga atgaaggcgc agtaccgcaa atttttattc
    19021 agggatttat tggttctagt tggttctttg aagggaatac tgacaaggga atcaaaaata
    19081 ttttggatag tctaggtgat caagaagaaa ttgaagtagt aattaattca aacggtggag
    19141 acgtatttca agggattgct attgggaact tacttaagtc aaataaagca aaagttaacg
    19201 ttgtgattaa cggcttagcc gctagtgctg cttcaattat cgcaatggct ggcgatacta
    19261 taaaaatcta caacaatgca caattgatga ttcaccgcgc ttccacatac ggagaaggaa
    19321 atgtcgatga cttccgtacg attgctgacc aactggaatc aattgataaa tcggtaaagg
    19381 cttcatataa aacacgattc aatggcacag atgaagcatt gcaagaactt cttgaaaaag
    19441 aatcgtttat ggatgcagaa acagctttga gttatggatt ggtcgatgaa attatcgatg
    19501 cagaaaatag ctcaggtact gaagctaaaa aagaacaaag cgttgaagaa attttgaatg
    19561 acgttaaaga aaaaagagca gaaaaaattg ctgcatttac agcagcatta aataaaacat
    19621 ttggacaagg agatgtaaaa taatgacagt taaaaattta aaaggtgtaa cagctgcaag
    19681 cgaccaattg atgaaagctt ttaaagatgg taacgaagaa tcttttagcg cagctatggt
    19741 aagcttatct aaggaaattc aggataaaat tttagaagaa gcaacagcaa aaaatcaaga
    19801 tcaattagta ttaatgaacc gtggtcagcg tgtattaact acacaagaaa caaaattcta
    19861 taacgaagta gtgaaaaacg aaggttttgc aggggtcgaa gaattagtgc cagctactgt
    19921 atttgaacgt gtatttgaag atttagaaca atctcatcca ctattgcaaa aaattacttt
    19981 tgttaacaca actggtgtaa cagaatggat tgtgtcacgt ggagtcaatc cagcatggtg
    20041 gggtaaactt tgcgaagctg ttaaaaaagt tttagataat ggctttgatg taattaacat
    20101 gaagcagttc aagctatcag gttatattcc tgtatgtaag gcaatgcttg atttaggtcc
    20161 agtatggtta gatcgttatg tccgtactgt tttagtagaa tcattgagaa ttgcattaga
    20221 acaagcaatt gttgatggta ctggtaaaga tatgccagtc ggaatgatgc gtgacatgag
    20281 caaacaaact agcggagaat atgctgaaaa aaaagcagaa cctattacag ctttagatgc
    20341 tgtaactatg ggcggtttga tggcgcgact atcaaaattc aatatcgaag gtgtgaatga
    20401 tccgatttat cgtaatgtga atccttctga tgtggtccta attgtgaatc caactgatta
    20461 ctggtctaaa gtattcccag ctaagactgt actaactgct aatggagaat acgtacaagt
    20521 attgccagta ccagtttcag atttgcagtc aacggctgtg ccagaaggaa aagcagttat
    20581 tggggtagcc tcagattact tcatgggtgt aggatctaca ctaaaaattg aagcttcaga
    20641 tgaataccat tttgttgaag acgaacgcat ttatctagct aaacaatatg caaacggtca
    20701 acctaaacgt aacgatagtt tcattgtatt agatattagc gctttgggaa ctactactac
    20761 aactacaaaa ccaacaacca caacaactac aacacaagcg taggtgatca gaatgaagta
    20821 tattctttgt cagccggcaa tcaatcggtt taaatgggag cttgaagttt gtttaactaa
    20881 tctgaagaaa ctaggaatca aagatatcgt attgcttttc agcagacacg atgatcagat
    20941 tcctattttt tttgagaagg aatatggtgt tgaagttcat gtgtacgatg atctgcggga
    21001 cgacaaagag tatattcctt cgattaaacc atatttatgg tggaaatatt tagaagaaga
    21061 tcattcgcgt gaggacgacc gatatttcta tatcgattcg gatgtcattt tcaataaaag
    21121 aattaatttg cgcaaattgc cttctaaaga tgatgtttgg tattgtagcg actgctgtag
    21181 ttatctaagt cttgattata ttagaagctg tgaaaacgga gaaaatattc taaaagatat
    21241 ggcaaacatt gtaaatgtta cagtagaatc tttggaaact ataaacacta attcaggagg
    21301 cgcacagtgg gttattaacc gtcctaaagc gaattattgg aaaaaggttt atctggattc
    21361 taatcggcta tatcgctacc ttagagggca aaaaacaaat atacaaatct ggacagccga
    21421 gatgtgggca cagctttgga acatgatgta tttcaatatt ggtcctaaag ttcacgagga
    21481 attagacttt tgttttgcta ctgatccaat agaaaaagtt aaagaagtaa aaatcttaca
    21541 caatgctgga gtaacaacaa acgatgaaga tttatttttc aaagggagat acgtgacttc
    21601 tacgcctttt gatgaagatt tatcatttgt aaacaagaaa aaatgctctt acgcatatgt
    21661 taaagcaatt aaggcggtgg ttagatgacg cctgaacaag tgactgaaga attgctaata
    21721 gctgtgaagg ataatattta cgttacctgg aacgaagaag atgagtcaat taaaaagatg
    21781 atagctaaaa atgctgttta tcttcaaagt aaagtgagta caacactttc tttttctcct
    21841 gaaagcttag aatacggatt gctaatcgaa agatgtagat acgactggaa tcgtgcttta
    21901 gatgagtttg aacaaaattt cgctagtgag ttattaggtt tcattcaaca ttatgcgcta
    21961 caagaatata ttgcaggtga tgtgaatggc gaataatcgt agactcgaag aaacattcaa
    22021 cgatggttgg ttaaagattt tgacgcaaac taccaaaaga aatgaactag gaaaaaagat
    22081 tggtgtagaa gatacagaaa tcacttcttt aaaatttaga aatctttcca tgagagatag
    22141 tgatataaca gctatggatg cgatgggatc gaaattaact aagaaagtaa agactccatt
    22201 tcatccaatc gccaagaaat ttaataaaga tcaatatttt atcgtaatcg atagtatgcg
    22261 ttacaacgtt atctatgccg attacgataa tttttatatc tatttttatc ttgaaagtgt
    22321 gggtgaatat ggtgattgat aattctaaag aaaaagaacg tttaaataag caaatttctg
    22381 ctatcaaaac ttccttagaa gagcattttg gcctcaaact ctttcaagac tccgtaggcg
    22441 aggatgagct acctgatgat tttaattact tcattctcga aacaggagaa atagaaatga
    22501 tcactgagcc aaaatatagc gtgggtcaaa atctatatct aactttctat tcagaaaata
    22561 gagaagattt aacaggagac tcactagata ttatttcatt gattcaaaat cgttcgattc
    22621 gttttcagag aatggatccc aatcatttaa aactagagaa ccaagatcgc tatatcgatc
    22681 aattggtatt tacgtttaga cgattattga agagtgattg tcatggctaa aaatagttgg
    22741 gagctaaaaa taaatggaca tgatgaactt cttgtgcgga tggaacgcta ttcaagcgag
    22801 agcgaacgac tgattaacga agcattgaaa tcaaaaggtt cggctattgc agtggatagg
    22861 attacagaaa aaattcctgt ttctgaagca gatttaagaa gagggcacca acacgcaaaa
    22921 aatagtcgtc cacttaagac tcaatacatt aatttgggtt tcatcattag acctacaaga
    22981 aaatttgagt atttaaaata tcctgatttg gggataggta cttctaaaag aaatcagccg
    23041 gacgaattta tgagaagagg attaggtctt gcacttgatc caattacaga acttctgatt
    23101 cgtcaattcg ataaattaaa taaatagggg gaacaacaat ggctaaaaca aaaactgtag
    23161 taacaacgtt cgataacgta agtatcaaac gaattgcttt taattttaag aacgcaggaa
    23221 atgcaatcgc aacgaattgt aacggacaat tagatggcga aacagaaatg caaacggtgg
    23281 ttaaaaaatg tggagcgaca gaagtaaaat caaaatctaa accaatcaat atgacggtaa
    23341 caattactgc acatgtaccg atggaagttt atcgacgttt caatgggttg aaacaagatg
    23401 gacgtattaa accaggcatt tactcttacg gtcctgattc cgtaggcgaa gatttctcac
    23461 ttgctgcaga gatcgtggat gacttcgaag aaaatagaaa gttagttggt atgttagcat
    23521 gcacttcgaa tacaggatta acattctcta ttgaaaatgg tgcggatgaa gtagctgcgt
    23581 tagaactaga aacaaaagtt atgcaagatg aatttggtaa attttatcat gaagcaattg
    23641 ttgcagaact tgaagaagac ttaacagatc aatggatgac gaatctatct gctgatgtga
    23701 ttaaaaaaac ttcagttgtg acaactacgg ccactcaatc acagtaaaaa aaacggaggt
    23761 agcgaaatga acgaagatta ctcaaaaatt gaactaaacg atggaacaat tttgaattta
    23821 gaacctaaac tgaatatcaa gaaattattg atgatcaata gagattttaa cacagacgag
    23881 tttgcaaaaa tgactgtggg aaaaggatcc atggatattt ctgttattca aggtgcaaag
    23941 gctgtgtata ttgcttaccg ccaagcgaac atgactgatt atatttcatt cgatgaattt
    24001 atcgataaat gggattttga tatggctact gccagctata tttatcaatt gatgatgttc
    24061 aaacaagcac gcgatgctta tcaaaaagaa tttgaaaaag caaataagga aaaaaagctt
    24121 caaaagtaaa aattccaaag ctcttagttg aaacgtgggt cgatgtctat tcgatgttga
    24181 ccgacgtttt ttctatgcct tcagatttgg ttttaagcga tatctgttta gatgacattt
    24241 tgcaaatggc ttacaacaag agtgcttatg aaggatggaa aaactatgca ataaaccaat
    24301 cccagaaaaa ctaaagaaag gaggtaaaaa atggctaaaa agagaacaga agcagaagta
    24361 actttcatag ctaacgatga cggattgaaa tctacgttaa aagaaatcag tgctgaatta
    24421 actaaaaata gagcagaatt aaaactagaa caagctcaat tacaacagac tggttctgaa
    24481 tcagacaagt taggaagtaa attatcttct ttagaaaagc agtatgaatt acaaagtcaa
    24541 aaagttgaag taactagcca acgtttagcc aatgcgaaaa aatattatgg agaaaattcc
    24601 accgaagtta agaaacttga aagagaactg attaatcaac aaacagcgca acaacgtttg
    24661 tcaaacgaaa ttgataaaac gagtaatgca ctagctcaag caaaaggcga aatacagacg
    24721 tacgagtcta caatgcaaca gttggatagc gaacaaaaaa atgttcaagc tagtgcttct
    24781 ctgattgaat ccgaatacaa aaaatggcaa gcaactgctg gtcaatcagc ttctgaagcc
    24841 gagaaattag cgaaagccca agaatatgtt tctcaacaat ctgaaaatgc ggagaaaacg
    24901 atagatatcc taagacgaca gttagaagct acacagtctg aatttggcgc tacatccaca
    24961 gaagcaatgc agatggaagc gaagcttaat gatgctgaac gtgaatttga agagttagga
    25021 caagctgcta aaaatgtaga tacaactaac ttggacgata tcggaagcaa aatagatatg
    25081 aataatttaa tggaagcttc tgacgtttta agcgacattg gcgataagct tacagaatta
    25141 gggaaacaag cagtggactc tgctaacagt gtaggtagtt cccagagtaa gatacaagct
    25201 aattttggtt tgtctaaaca agaggctgaa gaattaacga atgtagccag agacatttat
    25261 tataaaggtt ttggagaatc gttagatcag tccacagatg cattgatttt ggtaaagcgt
    25321 aatttaggag atttaaataa tcaagattta caaaatatca cggaacaagc tatggtccta
    25381 gaaaacacca tgggcgctga tatggatgaa acgttacgtg gtgtaaatgg cttaatggtc
    25441 aatttcggct tgagtgctca agatgcaatg gatttaatgg tttcgggtac tcaaaacggt
    25501 ttagataaaa cgcacgaatt aggcgacaat atggcagaat atagccaatt atggagtcaa
    25561 atgggatatt cagctgatga aacgttcgga atgcttcaaa atggtttaga tgcgggtgct
    25621 tataaccttg ataaagtcaa tgacttagtt aaggaaatgg gaatatcgtt aacagatggt
    25681 cgatttgagc aaaacatgga tatgtttagt gaaagtacta gaaaagcttt tgaagagtgg
    25741 aaaaatggcg gaggaacaca aaaagacgtt attaattcca tgattcaaga ttttagcaat
    25801 atggatggtc aatacgacca attaaataaa gcttcgacaa tttggtctgc gcttggcgaa
    25861 gataatgcga tgaaagttgt ccaatcttta actgatgtta accatacatt tgatgatgtt
    25921 agtggatctg cacaaaaaat gaatgaagat tctactactc cgttgcaaga gttgaacggg
    25981 aaaatagctg aattaaagga ttcattagct cctataggca acacaatcat agatgcactc
    26041 gaaccagtaa ttgattttct aggaaagatg gctgatgcgt ttaataatct tccacaacca
    26101 gtacaggatt tcattgttgc tataggtggt cttacagcag catttggcat actgttaccg
    26161 gcaatactag ccgtgtcata tttatttggt ccgatgatgc ttataattgg aggaattata
    26221 gctgttatag caggcgttat tgtggcaatt aagaactggg gtgcaattac tgactggttt
    26281 agtggtttat ggaaaaaatt tactgattgg ttgggtgata cttgggaaag tataaaagac
    26341 ggagcctcat cagtttggga tggggttaaa gaaacctggt ctggatttgt agattgggtt
    26401 caagatattt ggcaaggagt ttctgattgg tttggagagc tatggagcgg attagttgaa
    26461 ggagcttcca acatctggca aggagtccaa gaaacttggc aaacattcgt tgattgggtt
    26521 tcaaatattt ggaacggagt caaagaagta tggtcgatta tttgggcaga cattgtagga
    26581 attgttcaaa taccatggac attaataacg tcattgattc aagctggtat taatattatc
    26641 gtgggtattt ttgatgtagc tggacagtta ttgggcgcag cttggcaagc tgtttggaca
    26701 cctatttctg atttccttaa aaatacttgg gatactatga cacaatggat aagtatcgct
    26761 tggaatggga ttgtaactac attccatact atatttgatc cagtagtggc atggtggaat
    26821 ggtatatgga cagctattag tactacggct tcaaatattt ggaattcaat tagtgcaaca
    26881 gcttctagta tttggaacag tatcaagaat acaatcacta gcttggtaca agcagctgct
    26941 acagtaattc aaaatatttg gtcaactgta tctagttggt taggtggaat ttggaattca
    27001 atcagctcta cagcatcaaa tatctggaat agtgtgacta gtagtataag caatgctata
    27061 aacgcagcta aaagtgccat tcaaagtgtt tggaatagta tatcttcgtg gattagcgga
    27121 atttggaacg gtatcaaaaa tactgctttg aatctttgga atggaattac aagcactatt
    27181 agctctaaag taaacgatgg aaaaaatgca atttcaagcg gttggtccaa tctaacaggt
    27241 attgtttccg acatattcaa taatgttaaa agtacaattg ctaacatatg ggaaggcatc
    27301 aaaaagactg ttagcgctcc aattgattgg attagagaca aaatcagtgg catttttgat
    27361 aatttgaata tttctatacc acatattccg ttaccggaat ttatcatgga aggcagcttt
    27421 aacccgctaa aaggtcaaat cccccatttg cgtgtaaaat ggcatgctaa aggaggtatc
    27481 tttactaaac caactttact aggtggaatg aacggtgtcg gtgaagcagg acctgaagca
    27541 gttttacctt tgaaaagatc tgttttgcaa gaaattggtg atcgtatctt gagtagcaca
    27601 tcagtttcat ctagggcaca aacgattcaa cctgtgaata actacgaatt caatttcaca
    27661 attgatggta acgcagatga ggttactatg aagcaaacaa ctcaacaaat cattgatagc
    27721 attacaaaaa ttcaaaatga taatgcttcg gcatggcgtt aaacaggaga gtatttctcc
    27781 tgttttttta gtattaaaaa ggatgtgaaa aaatgactga ttgtatacat tctataatcg
    27841 atggatttcc tgattatttg cataaattgg ctttagctga aagaccaacc ataccttctc
    27901 caaaaagaca gagagttgaa acttctgttt taggtaggtt aggtggctta gtacaagatt
    27961 actcgtttga agatatgtcg tttacattgc actataacta tttagaggat gtggaagacc
    28021 atcaagcgtt caagcaatcg ttttatatca tgcgtcattg gttaaactat gcaaagaaat
    28081 tagaattctc tgacgatccc aacgtctatt acgttatcca gactatcgat attggggatg
    28141 cagaaaacga tattgttgaa tggggagagt tcgatgtaaa tattactgcg aaaccattcg
    28201 caagagttca agaagatgta cctataaccg tagataaacc acagtcattt aacttgctga
    28261 ataatagttt agaagaaagt tttccaaaga ttatcatcac tccttcagct acttcatgcc
    28321 agttcatctt aaatgattat gtgtttagtt tcgaaggctt agtagcagga actgatgtag
    28381 tcattgatag tgatttgatg ctttgctacg aagagcaatc ggacggagat attttagatc
    28441 ggtccaacaa aatgaagacc atgcaatatc cgacattgca agtggatatc aatcatttta
    28501 attgcactgg tttgagcaaa atacaaattt atcgtaacgg gttaaggtag gtgaaataga
    28561 tgatcgataa tttaataact atttacgata aaaatgacgc gaataattta gctgaacatt
    28621 tatatgatac gcaaggttta ggcgctttgt cagactggtt aacagctact gttagcaata
    28681 aactaaacgg agccgagata tttcagggta cttattcaat aaacggaact aatgcagatt
    28741 tgattgtaga aggacgtatt attcagtgtt atgtagatga aaatcgagca aaacagcgtc
    28801 tacggattta ttatgcaaaa acttctgtaa taggcaatac gatagaagta aaagctgaac
    28861 ctattttcaa tgatataaga aaatcggtgt tgaataaata tgacagcgga acagaaaaga
    28921 tcactgctac tcaggcatgg caaaacgcaa aagttttagc gaaaccagct atcccttcgc
    28981 agttttcttt ctcgtcatta gtagatacgc ttgctaatgt gaagatagaa aaggcgaatt
    29041 ttttagaatt ctttggtgga aaagagggat ctattctaga tcgatttcat ggtgagtttc
    29101 taaaagataa taacacatta cgtcatgaaa aaagtctagg tacggatcat aaaatcaaag
    29161 cgatttatac taaaaactta actggtcttg acttagagat tgatgctcaa agtgttttag
    29221 ttggagttta tccattcatt agcagctctt cagaaggaga agacgagatc actctaccag
    29281 aagaagttat tttcacggat tacgtggatg attatcctgc tggatatgtt tcttttgttg
    29341 attttaaaga caaagcgact gatgtagcca cattaaggga agctgctaaa gactggttga
    29401 aaacaaacat agataaacaa aaaccacaag tgagtggttc gattgaatta gtaccattga
    29461 ggcatcaaag aggctatgaa aaatttgttg atctagaaaa agtttcgatg ggtgacggag
    29521 tagatgtgta tcatccacag ttaaaagtga atatgtcagc gagaattgtg gaatatacgt
    29581 ttaatgttct aaccaattca tacgataaat tagttgtagg aaacgtcaaa acaaacttct
    29641 tagaaaacac agagaataat gtcagcaatt tgattaatga tgccattgat caattgaaaa
    29701 acggtggcga aatcagtgat ttactcaatg atattgtaga tcatcaaact gatatgatta
    29761 ctggtcaaaa tggtggttat gttttattag atcctaaaga agcgcctagt cgtattttga
    29821 ttatggacac accagataag aataccgcaa ggaatgtttt acaaatcaac aatgctggta
    29881 ttggtttttc taaaactggc attaatggaa catatgaaac ggcatggacg ttagatggcg
    29941 gattcaatgc ctcgtttatt acggctggtg aaatagtagg aattactatt agaggtacta
    30001 cattaattag tgatggtgct gattatagaa caagtattgc taatggcaaa atgacttggt
    30061 actcaaaaaa agttaacaaa gatattatgg agctagaagc acgtgattat gtaagtgctg
    30121 atgccggtat tgtatcatac accatgaaaa ctggtggtgg tttcatgatt agaaatccac
    30181 agggtaactt ggtttttagt acgtgggata atggtaataa cagaccgttt ctatcttttg
    30241 gtgcgcccaa tttcaggtat agcaatgcta gttatgtaac ttctggcgac ggtagttctt
    30301 taagcattaa tggtagtgcg ggtaactcat gggaatttaa ggtagctggt aggactatga
    30361 aatttactag tgatggtatg ctaacgttac caggttgttt ttttggttca tgggaagatg
    30421 ggaaacttgc taggtttgaa caatcaacgg tacaagtata taaagatttt actgttagag
    30481 gtactaaaaa ctcaactgta ccaacagaac attatggaca acgactattg aacgcttatg
    30541 aaactccaga atattatttc gctgattatg gggaagccgt tacaggtgac aatggtaaag
    30601 ttcgtgttga tattgacccc atgtttgctg aaacagtaaa tctaagtcgg tatatgacac
    30661 atgtgacacc tacagaacta gttttgtgtg ctgttactca tgaagatatt gaccatttca
    30721 tcattgaaac tagtaagcca aacgtattag ttagatggaa tttagtggca caccgtctag
    30781 ggtatgaaga tattagatta aaagaggata cagcatatga tagcacagtg cttgaccaaa
    30841 aacgttttta aaacgaagac aaggaggtat ataaatggct agcagtttat ataatttggc
    30901 tttagatttc agcaaagaat taaactacac caaagctatt atggctcgtc aaggtgataa
    30961 agggattacg gtgacggtta aaccgtttct aaatggcttg cagatggata cgagtggcgg
    31021 aacatttact ttaaaaggaa caacaccatc taaccgttac gtagataatg ttgcaactag
    31081 tgtaactagt gaagaagtca cgttttctct tgatggcaca tttatgagtg aagcaggata
    31141 ttataaacac tgctacgtag aatatagaaa agacaatcaa attttaacaa cgcaagatat
    31201 catttttttc tcactaggag tgtctgacat ttcgcaaggc caagccgatg aatatgtttc
    31261 gcaattagaa gagttgattc gaaagtacaa cgaaactttt gatgctttta tggctgaaat
    31321 caaaggtaga gtggatagct taaatcaaca gattactgat ttaactggtc aagctaaaac
    31381 gctacaagac aagttagatg ctctgaaaga agaaatttct aagttaggta acttacaagt
    31441 gatgtacagt aacagcatcg acttcgggga ctatgattat tcggagaacc cgaatttaat
    31501 gccatatatt acagaacctt gggttggacc tttgttaggt aatggacata cagttaaaga
    31561 tagtgttaaa agggttataa ctcatactaa aactagaaca gctaattccg gcgacatatt
    31621 atcgcttggc ttaggtattc cgtgtacagc ggaagctaat aataggtatc ttataaccac
    31681 tttacgacca tctacgacat acacattaag cgttactatg agtgtcggtt cagattggac
    31741 tggagagacc aatactatag gggttaggct tagatattta aatgaacaag gtggtataga
    31801 actgcccatt aatgcattaa taccagcaaa tgtagaacgc gataaaatgg tcactcatac
    31861 atttactggt ataacaaaag ataatgtaac tagcataaca aactgttatg ttgaaatatt
    31921 ctcacttaat agtgaatata aaggaacggt tagtgtcagc tatgacgtta agttaaaagc
    31981 acactaccct aacttactag atggccctta ctggttaggt aaagttcctt taggcgagaa
    32041 tattgctgac cctacagttg tctttccaca caaaactagt gaatatatgg tttatggtag
    32101 acgcaataca gaaaactata tcgctgacca aacatatacc atatcaatga aagcaactaa
    32161 actaacagtt caatcgtttg ccgtgtatat tgctgctggc cgcgttaaag taggagacat
    32221 gaagcctact gaggggttag ctaatacatg ggagttaact tttactgtaa ctaagcaaca
    32281 tatagatagt ggggtaacta attatctgga aatataccaa tacccgtcag ctacaaaagg
    32341 agctgttcaa attgagtggc tcaaactaga aaaaggcaat actcgaaccc cgaatattag
    32401 tgagtataaa tatcgtggta ctggtatgcg tgattcaaac aatccaaaag attatgtttg
    32461 ggatctagca ccagaatatg tcgaagataa tttggccaca gatattaaaa tttctgaaat
    32521 tactggtaaa gcaaacaatt ataccgatgg gaaagtatcg gagattaatt cgcagttgac
    32581 tgcttcaatt aatgaagtag acaccacagc taaggatgct caaacaaaag cgaatgctaa
    32641 tgcgactgct atagatgaat tagacaataa gatcgatgaa cgcattaatg atacagctac
    32701 taccacatta acagttacaa acgggaatac cggatcagca aagctttatc gtgaaggaaa
    32761 aacagtttct atatattttg tggctttaaa cggaaaaagc agtggtggaa atgattcaac
    32821 gatactaaca attccagaag gctatcggcc accaattagt tttgagcaac tggttggctc
    32881 gatagaccgt tctactttga acagtgctca gttatctatt ggtgcagatg gagccattaa
    32941 atggcgaaga aactcaagtt atggatcgga ttataccttt gcaattactt acacgattta
    33001 gaaaagcgtg aatcgatatg aaggcagcat atagaccaat tgaaccttac ggattcgagc
    33061 aaatcattgt gaatgatgaa gaacatttac cggaagaatg cacagaagtc gaaccaccga
    33121 ttccaaattg gaaaccgaaa ttcaattact gggagggaaa taaatgaaaa acatttggaa
    33181 atatggacgt actggcggag agtacgcagg aaaagtattg gacgacatgc ttgtatccgt
    33241 tccttacaca gatcagccac cgcttgaagg aattcgtgct gatggcgaac cgttaacaat
    33301 cgctgatcaa atgtttgatc ctaaattgaa tcaatggatt attttagcga acgcactaga
    33361 tcacaacgat ttaaacaatc tcaaagcaat gtatgagtcg ttagaaaatg agaacggcga
    33421 tttaaaacag atcaatgcca aactcatgct aagcgatgta gcaattaaac aggaaaatac
    33481 tgcattgaaa gaaaaagcgg atagtttagc acaaatcaat tcaaaaatga tgcttgcttc
    33541 gttacaaaat agcaaagaca tttcagaaat taaagagcaa ctaaatccag cttcaaaggg
    33601 aggtgagtag tatgtttagt tttagcgatg tgaaaatgat gtatgattgg ggctgtttca
    33661 ctaacgaaca agtaatggtt ttcgttccgt tgtgcattac tgaagaaaaa gcagataaaa
    33721 tcattagcaa agaagagagc gcatcttaat tgatgtgctt tttattttga ttcaaggagt
    33781 tgtcacatga ttaatttagg ggaatgggga gcgatagcag gatcaataac cgctatcgtt
    33841 tctttgattt tattagtaat aaaaccaatt actgcatctt tctcgaagat tactgagact
    33901 ctttcaaaag taagtcacaa tttagatttg ctgactaaag atttagaatc gagcaaatca
    33961 gatcgattga tgattcatga agaactaaag aaacacgatg aaagattaga tacacatgca
    34021 gaaaaattgg tagaacacac gcaacaaatt aaaactttat ttagggagag aagaaaatga
    34081 ataataaaac gttcgaagta ctaaaatggt tcgcactggt aattattccc gcactagcta
    34141 ctttcgtggg gttagttggt aaagcgctca attggcagta cacagatatc tgtgttgtca
    34201 tcattactgg ttttggcgcg tttttaggga gtgtgttggg tgtatcaaat cgaacctaca
    34261 aaatgttctc ggctgaaagc gaagaaggag gaaaca
//
LOCUS       DACEUZ010000014        31325 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.014, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000014 DACEUZ010000000
VERSION     DACEUZ010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31325
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.014"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(341..3490)
                     /locus_tag="IXG44_000602"
     CDS             complement(341..3490)
                     /locus_tag="IXG44_000602"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HAQ1657341.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDSQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDGVYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNEKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSSEQINRMIDQMEPVKKESYLDPAVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQLIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLAIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PDKTKGLIVTFRKPATMEKNVEDATKLYSKAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSPTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYLPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
     gene            complement(3503..4702)
                     /locus_tag="IXG44_000603"
     CDS             complement(3503..4702)
                     /locus_tag="IXG44_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAQ1657342.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLIDLVVRLNKSTNSSRLPKVEF
                     EDIVAGEGRLNKDVSQKFDNRKGIEFLPNDILYGKLRPYLKNWLKATFTGVAIGDFWV
                     FRAKKSDSDFIYSLIQADRYQKAANDTSGTKMPRSDWKKVSDTVFYIPNDLKEQQKIG
                     IFFKKLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWEERKLGE
                     LGKTQSGIGFPDAEQGGSEGTPFFKVSDMNNIGNEYEMRNANHYVSNEQIERKKWKPI
                     KDVPAIIFAKVGAAIMLNRKRLVTSPFLIDNNTMAYLFNNTWDIYFGKILFETINLPR
                     YSQVGALPSYNSSDIENISVKVPVKDEQQKIGTFFKQLDDTIALHQRKLDLLKETKKS
                     FLQKMFV"
     gene            complement(4692..6287)
                     /locus_tag="IXG44_000604"
     CDS             complement(4692..6287)
                     /locus_tag="IXG44_000604"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAQ1657343.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAIAPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVDVLEHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEMWLQGALEVFKHEQ"
     gene            complement(6871..7578)
                     /locus_tag="IXG44_000605"
     CDS             complement(6871..7578)
                     /locus_tag="IXG44_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1657344.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENG"
     gene            complement(7643..8479)
                     /locus_tag="IXG44_000606"
     CDS             complement(7643..8479)
                     /locus_tag="IXG44_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1657345.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(8497..9906)
                     /locus_tag="IXG44_000607"
     CDS             complement(8497..9906)
                     /locus_tag="IXG44_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ1657346.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(9920..11743)
                     /locus_tag="IXG44_000608"
     CDS             complement(9920..11743)
                     /locus_tag="IXG44_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1657347.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(12586..12912)
                     /locus_tag="IXG44_000609"
     CDS             complement(12586..12912)
                     /locus_tag="IXG44_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1657348.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(12958..13320)
                     /locus_tag="IXG44_000610"
     CDS             complement(12958..13320)
                     /locus_tag="IXG44_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HAQ1657349.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(13357..13572)
                     /locus_tag="IXG44_000611"
     CDS             complement(13357..13572)
                     /locus_tag="IXG44_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1657350.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            13699..14034
                     /locus_tag="IXG44_000612"
     CDS             13699..14034
                     /locus_tag="IXG44_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1657351.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(14110..16107)
                     /locus_tag="IXG44_000613"
     CDS             complement(14110..16107)
                     /locus_tag="IXG44_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1657352.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSFLIYLAKGAGI
                     DETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(16205..16480)
                     /locus_tag="IXG44_000614"
     CDS             complement(16205..16480)
                     /locus_tag="IXG44_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1657353.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            16712..17446
                     /locus_tag="IXG44_000615"
     CDS             16712..17446
                     /locus_tag="IXG44_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1657354.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            17485..18627
                     /locus_tag="IXG44_000616"
     CDS             17485..18627
                     /locus_tag="IXG44_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1657355.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(18722..20113)
                     /gene="sufB"
                     /locus_tag="IXG44_000617"
     CDS             complement(18722..20113)
                     /gene="sufB"
                     /locus_tag="IXG44_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HAQ1657356.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(20118..20588)
                     /locus_tag="IXG44_000618"
     CDS             complement(20118..20588)
                     /locus_tag="IXG44_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HAQ1657357.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            complement(20575..21810)
                     /locus_tag="IXG44_000619"
     CDS             complement(20575..21810)
                     /locus_tag="IXG44_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAQ1657358.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            complement(21807..23093)
                     /gene="sufD"
                     /locus_tag="IXG44_000620"
     CDS             complement(21807..23093)
                     /gene="sufD"
                     /locus_tag="IXG44_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HAQ1657359.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            complement(23106..23879)
                     /gene="sufC"
                     /locus_tag="IXG44_000621"
     CDS             complement(23106..23879)
                     /gene="sufC"
                     /locus_tag="IXG44_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HAQ1657360.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            complement(24076..24924)
                     /locus_tag="IXG44_000622"
     CDS             complement(24076..24924)
                     /locus_tag="IXG44_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1657361.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            complement(24948..25634)
                     /locus_tag="IXG44_000623"
     CDS             complement(24948..25634)
                     /locus_tag="IXG44_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1657362.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            complement(25627..26661)
                     /locus_tag="IXG44_000624"
     CDS             complement(25627..26661)
                     /locus_tag="IXG44_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1657363.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            complement(27056..27394)
                     /locus_tag="IXG44_000625"
     CDS             complement(27056..27394)
                     /locus_tag="IXG44_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HAQ1657364.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            complement(27396..27752)
                     /locus_tag="IXG44_000626"
     CDS             complement(27396..27752)
                     /locus_tag="IXG44_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HAQ1657365.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            complement(27758..28945)
                     /locus_tag="IXG44_000627"
     CDS             complement(27758..28945)
                     /locus_tag="IXG44_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAQ1657366.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            complement(29136..29981)
                     /locus_tag="IXG44_000628"
     CDS             complement(29136..29981)
                     /locus_tag="IXG44_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ1657367.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            30215..31180
                     /locus_tag="IXG44_000629"
     CDS             30215..31180
                     /locus_tag="IXG44_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HAQ1657368.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
BASE COUNT         9064 a         6565 c         5280 g        10416 t
ORIGIN      
        1 ggttacatgg tatctgaaac agctaaaacc ttgtcattat agcaaaaaat cactttatct
       61 ccgtttcatt tcagatacat ttcacttaag cggattccaa aaaaacaaaa gaaaaaggaa
      121 cccctagtta gcctatgctt tttattggtt cctctcaaaa cacggtcata taacaacggg
      181 gtggggtaaa tgaatgaaaa aggtggagga aacattattc aatagctgtc ttaaatactt
      241 cgttataaaa tgtgaacaaa taaaagacat ctatccctat tttctacact tccacttgac
      301 taccatcttt gaatcgtaca attttcttaa ctcttctcgt ttacctcaat tcattctcaa
      361 gtggaatcaa ctcatcatct aacattttac gccatgctcg cttcatttct tgtgggtatt
      421 taaatggctt cgcttctgga tgtttggctt tatatccttc gtaatcctta ctgttgataa
      481 ttgctttcat attaggaatt ggatcttcac ctggtagata ttgcagatta gaagcatgta
      541 attctttctc agaaacaaac cactcattgg aaaagacatg aatcaaatca tcactcgctt
      601 gtgtaaaata gcgacgttta atgacaaaga tatcttcatt actatctaaa gtccccacat
      661 caaatgcatt taaaatttgt cggtaaactt ctttgactcc ttctgtttta ttcaatttag
      721 ccaaagcttt ttcaatttct tctcgtgcat ctgaactatt cgcagcataa gttcctagta
      781 attgatcaat ataagttgaa tcaatatcat acaacttagt ggtattttca ctataaaatt
      841 cgatcgttga aaaatcttct ccttcttttt ctggttcttc ctctattaat gaacccttca
      901 cggtctcgta cacgccaatt tgttcttcca aaagttgaat ttgattgttc aaagttggag
      961 atttcaccat gtcatcattg taatcgtcat aagtcactaa cgattcataa gcacgattca
     1021 attcttgaaa tgctttgacg tactcgatac ggatttcaag tggcgtatgt tcatcaatgg
     1081 attccatgga tgaaacctct tttaacttac catatgcttg tttgaatctt tttttggatt
     1141 cctgataact tggataaatc aaacctgatt cttcttttgc ttttgaatac agttttgtcg
     1201 catcctcgac attcttctcc attgtcgctg gcttacgaaa cgtcacaatc aatccttttg
     1261 tcttatcagg atacgtacga ttcgtacgtg aaaaagcttg gatcaatcct gcatactcta
     1321 gattacgatc cacaaatagg gtttgaatcg taggggcgtc aaaacctgtt aatagacgat
     1381 ccactacaat cgctaaatcg atttgtttcc caaattgttt gaattccgct ctttttcgtg
     1441 ccaaacgatt attgatgtct ccgttataac gctcaatgtc tgctaatgac caggaagtat
     1501 cataatactg attatattcc tcaataattt tttgcatttc ttcttgttgg gcacttgaat
     1561 ctcggctatt ttcttctaat gaataggtaa tagcgattcg tggaaaatca ggatcctcca
     1621 tcgttcgtcc ttcacgaatg agctgattgg gaaactcttt tgttaaccaa tcaggctctt
     1681 tcgtcatctc tttgatggcc cgataatact ttttggccat gtcaatggaa ctcgtggtta
     1741 agattgcaga tttcgttggc ctgccatttc gaaaatcaaa ttttgtatag gcattatctg
     1801 gacggaacat tttatgaatg actttttgga tatgttcatc tgcttcaaaa acagctgggt
     1861 ccaaataact ttcttttttg acaggttcca tttgatcaat catacgattg atctgttctg
     1921 atgaatactc tgcatatgtt tctacttctt ttaatttccg atagatttta ttctctagtg
     1981 aagtcggctc cacggtgttt tcatgctcta cttgaaatcc taagacagag ccatcttcta
     2041 gcgcattctt aatggtatag gtatgcaaga cctctccata ctgatcatgg gttgtacgtg
     2101 ccaattgccc tttggcttgt ttccgattct caggaaaaat cggtgtacct gtaaagccaa
     2161 accaagtgga tttcggaaac atttttttga tttcttccat attttcagca cttaacgctc
     2221 gatgacactc atctactaca aacacgatat gttggcccat taatttttgg aagcgattgg
     2281 ttcctttttt ctcttcttgt ttctttgcat atttcaacgc cgcatctagt ttttgacggg
     2341 ttgtaatgat tacaacattt gcattggcat ctgccaacag ggtttcactt aattctttgg
     2401 cacttcccgt tccaacgatc aagctattcg ctttggcgtt cccggaagaa atcccagtat
     2461 taaattccga agcaaatttt gtaaactctg tggtggtttg attatctaaa tctttgcggt
     2521 ccactaacat gatcgttcga tcgatccccg attttcgtgc taaaagctta gtagatacaa
     2581 aactagtcaa cgtctttcct gaaccagtcg catgccacac ataccctgat tgatgtttat
     2641 ttgcggcaat aaacaaggct tgaatcgcat gtacttgata aggatgtaac accatgaggg
     2701 ttttattatc ttgatcttca ctgacgattg tataatccgc aatcaaacga tgagcatccg
     2761 ggatatttaa cacttgttta caaaactcat ataagttttc tactttttca ttgtctttcg
     2821 ttcgccaact aaataaaaac ttagggtgca agtctttcgg taaggcattt gcaaaatacc
     2881 gagtggtttg ttcattggaa accacaaaca actgtaaggt cgaaaagata ttgttccgaa
     2941 acataccttc ttctgcatat tttttgattt ggttaaatgc ctgataaaca ccatcttttg
     3001 cagttacttg tttcagctcg atttggacaa tcggtaatcc attgattaat aaggtgacgt
     3061 caaaacgtcg atcccgtgct tctatgttac ttccacgttt tgcaatttga tggaccactt
     3121 cataagtaga gattcctcca ccaatgtctt gattggaata aaggataagt gagacagaac
     3181 ccaattgcga atcttcccgt tcaatcgtga tccgagccat cccattttca ccttttaacc
     3241 atttcgcagc atcaaatggg gttttcgttc gcagcagtaa ttccgttttg atcgtttcaa
     3301 attctttatc ggtcaaagga aaatcattca gttctgcttg attatttgag ataatttttt
     3361 ggcgtaaatt gacccaaagg tcttcttctg attttagatc cggacgatac gtccactgat
     3421 tatgcccttc tccaagtaca cgaatcaact gttcttcgac ttctgcttcc tcacggtgac
     3481 taattttcat tcttcttccc tcctagacaa acatcttttg taagaagctt tttttcgttt
     3541 cttttagtaa atctaactta cgttgatgaa gagcgatcgt atcatctagc tgtttgaaga
     3601 aagtaccgat tttttgttgt tcatccttta caggaacctt aacactgata ttctcaatat
     3661 cagaactgtt ataagaaggc aatgccccta cttgggagta tcttggcaaa tttatagttt
     3721 caaacaatat tttaccaaaa tagatatccc aagtattatt aaataaataa gccatggtat
     3781 tgttatctat taagaaaggg cttgtgacca aacgttttct attaagcatt attgctgcgc
     3841 caactttagc aaatataatc gctggcacat cttttattgg cttccatttt ttacgctcaa
     3901 tttgctcatt agatacataa tgatttgcat ttctcatttc atactcatta ccaatgttat
     3961 tcatgtcgga aactttaaaa aatggagtac cttcagaacc accttgctcg gcatcaggaa
     4021 atccaattcc cgattgagtt ttacctaatt ctcccaactt acgctcttcc caatcttccg
     4081 taaaaccagg aaaccggatt tctggcactt ttgctccgtt ttcagggaac attttttgta
     4141 agaatccttt tttcgtttcc ttcagcaaat ctaacttacg ttgatgaaga gcgatagtgt
     4201 cgtcgagttt tttgaagaat atgccgattt tttgttgttc ttttaaatca ttgggaatat
     4261 agaatactgt atctgaaact tttttccagt ctgaacgagg catttttgtt ccagatgtat
     4321 catttgcagc cttttgatat ctatcagctt gaataagaga ataaataaag tccgagtcgc
     4381 tcttcttagc tctaaaaacc cagaaatccc caatcgctac gcctgtaaaa gttgccttga
     4441 gccaattttt taaatatgga cgtaactttc catatagaat atcatttggt aaaaattcaa
     4501 tcccttttct attatcaaat ttctggctca catctttatt cagtctacct tctcctgcca
     4561 cgatatcttc aaattcaact tttggaagtc tactggagtt tgtactttta ttaagtctaa
     4621 caactaaatc aattaactta cgctcttccc aatcttccgt aaatcctggg aaacggattt
     4681 ctggtcgtgt atcattgctc atgtttgaac acctccagcg caccttgtaa ccacatttcg
     4741 ttttcaggtg ttgtttgcag ggaagagata gcttctaaaa gttctttttc tagcacttgc
     4801 tgttcttgtc gtatcttctt gatgtcattt ccgacatgaa ccatatcaat tggttcttct
     4861 tcttcgaaag tatctacata gcgtgggata tttaagttat agtcattttc tttgatttca
     4921 tcaaaggttg ccaaatgagc gtatttctcg acatcttttc tttcagcgta ggtttcgagg
     4981 attttttgaa tgttttcttc cgatagttta ttttgatttt tccctttaac aaattcacga
     5041 ctagcatcaa tgaataatac atctcgagtt tgacgattct tcttcaacac aatcaccgtc
     5101 gttggaatcg atgtcccaaa gaacaagttc gcaggcatac cgatcacagc ataaatactg
     5161 ccatcttcta gtaatttttg acggatgacc ccttctgcag cgccacggaa taaaactccg
     5221 tgtggtaaga cgatggccat ggtacctgtt tccttcaaat gatagaaccc atgaagaaga
     5281 aacgcaaaat ctgctttgga ttttggcgct aatttcccat aacgattaaa ccgagaatca
     5341 tctaagaaag tcgtatctgc tgaccaatta gccgaataag gtgggttcat tactactgca
     5401 tcaaatgtgt aaggttcatc tgttggccag tccttattca acgtatcccc attgcggaga
     5461 ttcatttctt cggcgtcgac accatgtaaa atcaagttca tttttgctag attgtatgtc
     5521 gttgtattta gttcttgccc atgatattta acgttatctg ggtgtgttaa gtagttacgt
     5581 acgtttaaca tcaatgaacc tgaacccatc gttggatcaa aaacactaaa gaatcgtcgt
     5641 tccttttgat cgagcgttac gatttgcgcc atcatatcag aaaccatatg aggtgtgtaa
     5701 aattctccgg ctttcttccc tgcttctgaa gcaaattgac tgatcagaaa ctcatacgca
     5761 tccccgatca cgtccccatc gtgttctaaa acatctacat cattcaattt cttgatgact
     5821 tcggtaatcg tcacatttcg ttgttgctca tctgttccta gtttttttga ttggagatcc
     5881 acatcatcaa ataacccatt aaattgattg taggtagatg ccaattgcac aaaggctttg
     5941 tttagatcgt tcaattggaa cgttgcttgt tttgcttgat ccgctaagac gttgaaaaga
     6001 tactctggtg caattgcata tcccaaaata tcaacgattg tggcaatcag atcttctttt
     6061 gattcttcat ctgaaagtaa ttcacgatac aacattgttt gtttgcttac tgtgtcatat
     6121 tcctctaagg attcatctgc taaaagaacc acttgttcca acaatcgatc ggataaatat
     6181 ttatagaaaa tcagtcccaa taaataattt ttatattctg acgcatccat tttactacgg
     6241 agattatctg ccgcactaaa taatttttga tttaattctg cacccaaaaa gatacactcc
     6301 tatctcttat ttaaagttgc tatataccct cttattttag cataccccct tttataaaaa
     6361 gcaaaaaaaa aagaaagaaa cttattcttg ctaataaact acgcgaaagg agtctaacgt
     6421 tgtttgatat aacttagact gatcgttaac ttatttttta tatttctaca tagttatctt
     6481 tagaaccatt tcagatacat atgaaacaat tacatatgat tttttcataa aaaaacagta
     6541 ctgtctagta gtataaaata tacaaatttc ttaaaaattt tttcactaaa atcactacct
     6601 gaaacagtct aaaacactta tatatcaacg tttaatgaaa ctatctccgt ttcatttcag
     6661 atacaaataa atatagtcgg gctccaaatc cgaaaaaaaa tacccccagt agcttatcct
     6721 aagctactgg agatgtataa gtacggtcat ataacaacgg ggtagggtaa ataaatgaaa
     6781 aagatggaag aatcattatt caataacttt tttaaatact tcgttgtaat cagttacctg
     6841 tccgttctta aataccacag aataatcttt ttacccattt tcaagtaacc gaagtggaat
     6901 gtctttacca acttgctctg tcactgcatt actgatacct gttagattag ctaataaatc
     6961 tgataaagat tgagcatctg tagaaatctc tgtatcagta tttatcgtga ttcctttatc
     7021 ttcatttaca agcttccaag taggtggtat agctgtgtat gtttcgatga atgaatcaat
     7081 tctttcatct atcgtcttcc cagtatctac ttctgtctca ttactcttag atgaatcgat
     7141 tctttcatcc atcgtctgct ccgtaactgc ttctgtctca ttattctcac cggaaactct
     7201 cttcacaccc aaacttatat attgtccatc tttagtgtgc atagttaaaa ttcccacgct
     7261 tttactaata aattcactat aaccagttct ttcaatagag acttcaatgt atttcacact
     7321 atcatactca aaagaaaaat agacatgtaa aagcattttg ttgtttacat ctacataata
     7381 atcctttgac tctacttgag accttcctat acggaataca gctttactgt ctgatatctc
     7441 tataccagca tccttattat taatctgcca atctccaagt agctcttctg gcaagctagt
     7501 agtttcttgt gaacacttta ccacttcact ggtagaggtc gaaacattca cctccgcact
     7561 gttagaagaa ttttccaagg aatcaggagc cttagcacaa ccaacaagtc tttatatatt
     7621 gtgcagccga tattttcttc attcagtaca cccttgaaat atgcagtgtt agataaagca
     7681 gttcgtcctg atttgctgag gcacccatct cttcttgaac gtactccttg attttcaagg
     7741 ccgcccgata ctctttcgga aaacgctgct tcactgaagc cagcaaaaaa tcatccttct
     7801 ccggcaactc ttccttctta atcattcggg caataaaaaa ctgtaaatgc gtcacaaatc
     7861 ttaagtaaag catcgaatct ttatcaacct ttttttcggg aaacagatat cctagcatag
     7921 ccaagatatc ctttagtgct cttaccgaga gcatggtttc ttccatattt gcttgttcac
     7981 tttgggcatt tacaatgtga aacgcaatgt ttccagcctc ctcttctggc aactgaacat
     8041 tcattttttt gttgatcaat tggatagcat agcagccgat ctcaaattct tttgggtaat
     8101 acttttgaat ttcaatcagc aggcgatttt gaaaaacaat gcctttttga ctgcgttcaa
     8161 tggcatgctt caagtgatca accaaggtaa aaaatagact tttattcagc ttaaagtcaa
     8221 acttggcgtt ggcataatcg ataattcgct tgacgcaaat aattagatca tccggagcag
     8281 cctcaaacag tttaatgtaa tcctttttta gagcatcaga aagtgaatct aatttaaata
     8341 ctttttggat tttgtcagcc ggaaccaaat cgcctttctt tagttgaaat ccaatccctt
     8401 tccccatcaa aattacttct ttgccttgct tatctaagca aagaattaaa ctgttgttta
     8461 acgttttaat tactctcatt agattcaccg ttctctctaa gctaagccat ttcttgcgat
     8521 tactttttga taccaaaaga agctttcttt ttttattcgt tttaattctt taagattctc
     8581 atcggttcgg tcaacatata taaaaccata acgcttattg aagccttgat gggtactaac
     8641 aacatcaatc gctgaccaag gtaaataacc aaaaacatct acaccatctg ttaaagctgc
     8701 ttgaatagcc agaatatgtt tggctagata atcaattcga tcctggtcat taattcgtct
     8761 atcttcctca actatgtcgg gtgccccata gccattttca gtaattaaaa taggtaaatc
     8821 atagcgatcg tacaatttcc tcaatgttaa tcgtagtcca gtagggtcaa ctacccagcc
     8881 atatggcgtt ttatctacat aaggattttc agctgcccgg tagacacctt cttctcccaa
     8941 catgatctgt tgatcccccg ctctagcgga aacatctgat ccgtcatttt tgctggcagc
     9001 gatggtggca gttgaataat aattaatcgc aataaaatcg ggtttaccgg aggataaaat
     9061 tgcctgatcc tctgcctcaa ttttaggata taatccacga tcattcaaat agttttcaaa
     9121 caaataattg tattttcccc gaactgctac atctaagaaa gaccagcctc tcaatgtttc
     9181 ccaattatgg gctgcaattg catcttctgg actggaggta gcttgataca tagccgttaa
     9241 attgattgct ggaccgattt ttcctttaag attcaactga tgatacaagt gaattactct
     9301 agcttgagct aataaaacat ggtgattaat ttgaaagagt tctttttttg aaggcagctc
     9361 tccacttggt ggcaaaccaa tcgctccagg atgaaggatc atcgtgtttt gctcattaat
     9421 tgtcagccaa tagttcacct tgtcaccata ctcagtaagc aatacctgta catagtccac
     9481 aaaataatcg atagttgctc gatttaacca accgccctgc tcctctagaa aataaggtaa
     9541 atcgaaatgg tacatggtga caatcggttc gattccctga ttcaggcatt catcaattac
     9601 ttgatgataa aaagaaattc ccttttgatt ggttttatta ttttcaatat ccataattcg
     9661 cgtccaagcg atggaaaagc gaaaagcttt taagcctaat tctttaaaca gctgtatatc
     9721 ttctttatag tgatggtaaa aatccgaggc aacttcaaaa ttagctaccc cttctgtatg
     9781 ctgatacttg tcaataatag acggttgttt cccatcctcc tgataggcac cctctgcctg
     9841 gtaggcacta atggaggcac cccataaaaa cttctctgga aatgctgaaa gctgtttgta
     9901 ttgcacaaaa atcctctcct taactaattt gcatcaatgg ttcgtgttcc tttaagcttc
     9961 cgcttgcttc actaattcct gcgtaatttt cactgttggt cactaccaca atcactgtat
    10021 catcgtagcc agcgtttcta atggcagcga aatcggcgtt gattaataat tgtcctgctg
    10081 acacctgatc tcctgctttt acaaatactt caaagggttc cccttttaaa ttcacggtgt
    10141 cgatccccac atggatcaat aattcaatac cttctttaga acgtagacca atcgcatgtt
    10201 tagattctaa aatagtaatg acttctccct caacaggtga ataaatgctg ttttcttctg
    10261 gatgaatggc tacgcccttt cccaatagtc ccttagaaaa gacagcatcg ttcacttcag
    10321 aaataggaca cacggttcct tttaccggat tttcaacaat gactgcttcg gctattgccg
    10381 tttgctctga ttcatcagaa gtatcgtcaa atccaattag ataagcaatg ataaaggttg
    10441 caacaaaggc gattacacac gtaataattg cgtgaacaat attcattgga ttttctccaa
    10501 taaaggctgg taaggctgct aagccagggg aaacaaaggc atatctgact aaaccgctca
    10561 agccagcata caaaccagca attgcaccac caatcatgga agcaactaat ggttttttca
    10621 gcttcaaggt tactccatag agtgaaggct cagtaattcc cattaaagca gtgatcccag
    10681 tggacacagc tagctgcttc aactctttgt ttttagtttt cgcggctaca gcaaaggtag
    10741 cacctgattg agcaatatta gaagccagca ttcctggccc attgatcatt tcagaacccg
    10801 tactgctcaa ttgcatagta gcgattggcg tcattgccca agctgtaccc gtaaccacca
    10861 aaaatggctg caaaccaccc atcaaaaatg gaattaacca agctgctttg ccgttaatcc
    10921 attcagcccc cattgctata agatcgttca gcaacgttcc aaacggtcca atgacaacca
    10981 acgctaaagg ccccataatt aaaaatacca gcatcggctt agtaaagaat ttaataaacg
    11041 aaggcgaaat tcgatccgca aagggctcta cataggacat gatccaaaca gtaaagataa
    11101 tcggtaaaac tgatcccgca taatctgcag ctaataccgg aattcctaga aaagaaacgg
    11161 catcgccagt agaaaaaagc tgtgaaatat ttggatgaag caaaatgcca gcaacagtaa
    11221 tcgccaaaat tgggttgaca tcaaacttta tggctgcccc ataggctaat agtactggca
    11281 taaaataaaa ggctgcatca gcaatagtat tcaaaatgat gtaagtttga ctactgtctg
    11341 atagtacacc agtcaaagtc aagacggcca aaatggcttt taccatcccg gcacctgtta
    11401 tagctggaat catcggtgta aaggtggtag aaatgacact aatcaatctt gaaataatac
    11461 tttcttgatt gtctgaagag gggccctttt taattgaagt tcccacttcc ttttgaattt
    11521 ccttgaaggc tgtttggact tcattgccaa tcaccacttg gtattgtcca ccgctatcca
    11581 ccacactaat tacaccaagt gttttttcta aggctttcgt atcggctttc cttttatctt
    11641 tgagttcaaa acgcagtctg gtggcacaat gggttaagct gaccacattt tcttttcccc
    11701 caacctgttg caatacattt cttcctactt ctctgtaatc cattattctc tctccttaaa
    11761 gcagtaaaaa agaccaagtc aaaccacgcc ccaatcaaca ttgaagtgtt agtttgtctt
    11821 ggtcttgcct gcataatcag taacatgcca ataacgttat tttgctgtaa tccaataata
    11881 gcacattatg atagcgcaat caacagggaa tttaattttt tatcttttcg tttgaccgta
    11941 tagggtaata taacagttag atcacatcaa ctagggtata tctgcgtata cttactgcta
    12001 aaataagtat atctaatatt aactatacca gtaaaaccat tcctacataa ccagcgggaa
    12061 attcccaaaa tcgtgaaagc atcaaaaatg catataacgg atcacacaac ttcaacaatt
    12121 ctgctcttgg gaaaaattga tgaatttggt gagtaaagga tggaataaac agcattccaa
    12181 caatataggc aacaatagtg atacttgttt ttttggtaaa aatcagtaag caatagccaa
    12241 tttcaatcat tccgagatat aaaggtatca atagaatgat ttgggctagt attcttaaaa
    12301 attaactagc aaatgtttaa actgatttat ttctaatcta catgtagtaa gtatttcaat
    12361 tccaccaatt atcaataaaa ataccaaaca tatccataat aattttttct ttttcatttc
    12421 ttttatattg ttataacagt gaataacata aaaattcccg acaatcattg taatattttt
    12481 ttgtcacctg tattagtatt taatttatag gaaactataa aaactcccta gtgaatttac
    12541 acctttggga gttcctgttt ggacggtatg cataatacat ttaaatcaat ttaacattaa
    12601 cacctcagca tatggtatct ccacatatat acggtctttc tttggatagt actcaatatt
    12661 tatgttgata gcacttccat tgtctaatat agctttagcc cggtatgtgt tatctccatt
    12721 ttcatgagta atcgtcacat catcacatga tcgatttaag ttgtactgtt cttgtaaaat
    12781 ctgtgttacc aatggttttg atgacttctc taatccagat gtacctgtca tttttgaaaa
    12841 aataatacct ataatagcca agacaacaat acctataatg acaaacttct tgttcatatt
    12901 tactttattc aactttttat ctcctatctc caaaataatt tgttcttatc cctcttatca
    12961 gtacattgac atccctaaaa gtacaccaaa gattgctcca gctacccctg ctatcatcaa
    13021 aatcgtaccc attttctcat ctttttctcg atataagtag cccataataa cagcaacagc
    13081 accaaataca acaggaataa atgctaacga tactaccata gaaatccaac caataatcaa
    13141 atacagcttc cctgtctctt gtccatgtac gatttgttcc ttcatttcat taattgtctg
    13201 ctcagtactt ttctttactg tgtctgttcc ggttgtctca tctgtttctt gtagtgctcc
    13261 acaaaactgg caatacttac tttcatctgt tatttctttg ccacattttc tacaatacat
    13321 gtcttctcct cctaaaatca ttcaattgct tattagttaa ctgtttactt tattaaatac
    13381 atcagaaaac agctcattca ttggtacact taatatctca gaaagcatct tcatttgata
    13441 gggtttagga atcgttctcc ctgtttgcca cgaacttaca cattgctgtg taacattcat
    13501 tttttttgct aactccgttt gagaaagtcc tttttctttt ctgtatcggc tgagattact
    13561 tacttttttc atgattctcc cctcctgttt tattatatac aacaatatga tgtatttcaa
    13621 ctcataaaat ataaaaaact tcaaatacca ttacaaatac aacaacttgt tgtactatac
    13681 tgagatgggg gtgtattaat gaaacagaaa caatatgcaa aggtcattag agaaaagcga
    13741 aaagctctcg ggtggacaca aaaagaatta gctgaaaaaa tcttctctac acaacaggca
    13801 gtagcaagat gggaaaattc tgtaacagag cctaacctcg atagcttaac agctctttct
    13861 agagccctag gaacccctgt aagccatttt ctagacaatg ttgtcgtaga ttatgaggaa
    13921 gagtttttgg ctctctatcg ttctcttagc actgaagatg cagttcgaac aattgactat
    13981 atgaagctat taaaaaggca agaaaatgaa cgcaatcagc tattgaaaga ttgaaaaata
    14041 aataacccac cgaactttaa cggtgggtgt attgagatga aataagatta agagctatgg
    14101 ataacctgtc tagtttaata caattgggtc ttttctcttc cttactctcc gtccatcatc
    14161 tcttgttaca cttgtaaatt gatacagagg aactatccca gctcctgata atatcaattc
    14221 ttttattgtt tcatctatcc ctgccccttt cgctaaatat attaaaaaac tactacttgc
    14281 tttcacattg tatagtttct taatatcctt agaagctcgt tcaagagtga atccttgttt
    14341 aacaaagtag cctctgtttt ttattaaagc atttttcacg ctgttatccc caacattacg
    14401 taggcgttca caaacagtgt taataagttc tttctctgtc attagatatg tcccttgcca
    14461 cgataagccc tgtaaaactt catgtgcttc atctaaaatc aattgttttt ccttgctgag
    14521 ttctgatcgg gaattttgag gaaatactct tttagcctct ttttcgttaa aacatatctt
    14581 aacccattca taagaaaaat attgatatgt aaaatgaatt ttttccagaa gtggaaggat
    14641 atcttcattc aatttttgaa tcacagaagt tgcattaaaa acgtctaatc gatagacatt
    14701 gcttctctta tgattaaagt ttcctaacgc tttctcttcc tttaacctat ttaggtattc
    14761 gagcatcttc aaaggaacat cttcatcatc taattttttt atcaatccta taaaagtcat
    14821 aagtgatagt aacttattac atttatctat gtcataagtt ttaccatagt aagaactcat
    14881 ttttttagct aattgttccc ccgacaaaat acaagtcaca ctttccttac tatcatcaaa
    14941 aaaattaaca tcagaaagca agacatcata gacacgtcgc atttcctcta gataattcct
    15001 tattgtcctg aaaagtttgt ctttcattga ttcaagagtg tcaaaaagta cttttgattc
    15061 ctctttcaat tttcgaaaat gtgtaggata caagtctagg ttctctaata gccaattaaa
    15121 tgtttctacc cgactgtctt tctttaaaat tagcatcaca actccaataa aattttcact
    15181 gatatcacaa ttactggaat gacaataaaa aatggaatgc ccagttttac tggtaaaaat
    15241 actagcagag ggattatgat cgttgtgaaa cagacatctt aagtttttac ttgacttaag
    15301 taatttattc atatcaatgg ttaacaatct ttcataaatc tcattaaaag acatgtcgga
    15361 ggaatcaaac aggcagtctt taaaccactg tttcatttct tctttgtcgt cattttttat
    15421 caacttaaca aaatcggttt cattgactag ttcatactct ctccctgaat cactttgaca
    15481 atttttagaa catgtcatgg taaaatcaat cggaagctca gtaatatcta acatgttgtc
    15541 ttcattaatt attatcactg catctgactt atatcctcca aaaaacattc tattcgaagc
    15601 atttacattg tcatcacacc cggggatgtt ctttttcaag tattcgtaaa cagcaaccat
    15661 tttaccatag tcagtaattg cttcttttaa tagaaagaca atcttgtatt tttctagatt
    15721 ttcagagtac cgaatacttt tttgtatcgc aaacgcagaa ttatgaacta aattgataga
    15781 taaaaccttt tctatgctta agtatctact atcatcttta ctcaaatcta ctaccttccc
    15841 cttctcatct ttaacagtgt tatcgacatc cagaataatt actctggttt ccacaaaact
    15901 ctccttgtca ttgcctttat tctttgtctt gcttggaata aatgattgtg atgaatttag
    15961 tatctctaca aactcatctc ttgtaacttc tctatagtta tctaaacacc tatctcttac
    16021 atcagttttt tctttttttg taactggctt ctctaataat ttttcttgat ctacataaat
    16081 tggaatatta atcattgatg tttccatttg tgtttcctcc ttataaaatt ctgttaaaga
    16141 ctaaaacata cagataataa aaacaaactc tttattacta tatgttttag tcttatttgt
    16201 ttactcaacc tttataccgt tccatgaaac tatcaatatc agatttttta aatctaatga
    16261 ccgtatcaat ttttataaca ggtaacccgt attgagaaac atattttcta atcgttccta
    16321 ctgatacacc tagatattca cttgcctgct gatagtccat atactctaga gtgactccag
    16381 aaataatgtc ttctagcata ggctttagca ctgtaattat ctctttcact tgttgttcaa
    16441 cttgtataga aacaatgcta tcaatgattt gtagagacat atttccccct cctttcgtga
    16501 ttgcttgaac aaattttcta agaattatct tcgtattttt tgctcaaaat aaaagtattt
    16561 tgttattaac cgattaatca aacaaaaaat gattaccagt tcatcgacat acacataata
    16621 cctcgataaa aaaggggtgt caacagcttt gtaaaattta tttacaacac cccttttttg
    16681 aggtatacta attttaggag gtgtggtcaa tatgataaaa cgtccaccaa taaattattt
    16741 agaacgaaaa aagatactcg gaacaaaaat aaaagctata agaaagagta aaaaattaac
    16801 acaaccagca tttggtctta tgattaacaa cggtcaatta attgacaaaa aaactatcta
    16861 cgaatgggag aagggaacct atttaccaat accagagcga ttatcccgaa ttgctgattt
    16921 gggaaatatg agtatagagg agttagtttg tggcaatgta gaagaatata ttttgggaat
    16981 tattctttat agagatagca ttgtactaga tggtataaca tttccagata aaaacctgtt
    17041 ccagcatctt cgtcaacagt tcccaccagt acactctaat ttagatacat ggctggatag
    17101 atattccaaa ttagaaccag agatgcaaga atttattgct aacaagactt gtaataaagt
    17161 taaaaatgaa aaaattagtc ttttcaatat cttaaaaata gaagaattat tcatcaatgc
    17221 tattgtagaa gaatttgaca ataacattct tttcctcact agcagtatag aagaactact
    17281 agaaagaatg gtagatgaat ggctacctat tcaattgaaa gacatgtctt atcccgaaga
    17341 agctgtgaga gaaattactg ataatataaa caagctagaa caaacgattt catcaattgg
    17401 gaaaaaatac actaagaaaa aaatgaaggg aggtgatacc atctagtggt aagtactcct
    17461 tgtaaactaa gaggagatga tttaatggca aaaatatcaa agtacaaaaa gaaaaacggt
    17521 caagtggcgt ggatgtataa aggacacatt gccaccgatc cacgaactgg tgaaaaaata
    17581 aatactacga gaagaggttt tagtagcaaa caagaagctc aaaaagatta tgacgagtac
    17641 agacatcaaa tattatacgg tgttaaaaag aaagcacctg atatgacatt tgaagacctg
    17701 tataatgaat ggataactca tcagcgtacc agtgttaaag cttcaacaat cgcaatctca
    17761 gtaagatacg caaataatca aatacttcca gcatttggta agcttaaact atcgaatatc
    17821 tctgtcccat attgtcaaaa agtggtagat gaatggcaca gtaaatatga aagttacgat
    17881 tatatgagaa aacagactgc acaaatttta cgctacgggg ttgcaatgca atatattgac
    17941 aataatccta tggaaaaaac acttcttcca cgaaaaaaag agtatgagaa aaatcgtaaa
    18001 ttttactcaa aagaggagct taataattta ctggatgctt ttaaagattt tggtaacatg
    18061 aaacaatacg ccttcttcag attattggca tacacaggta tgagaaagag tgaagtactc
    18121 gctttacagt ggaaagacat agacacgttt aataaagaac tacatgtcaa taaaactctt
    18181 gctgtagatg aatttggtaa agttataatt caatctccga aaacaagagc ttctagacga
    18241 gttatctctt tagatactga gactctttct atcctcaaca actggaaatt acagcaaaaa
    18301 gaagagtatt taaaacttgg atacaatact agctctaaag agcaacatgt ttttacaaca
    18361 gttaaaaaca ctctatacat tccaaacact gttaatgatt ggttaagata tattttgaaa
    18421 aaatataact tgcctcgtat tacacctcat gggtttagac atacacatgc tagtttatta
    18481 ttagaagcag gagaatctgt caaggtagtt caacaacgat tagggcatga aaatagtaaa
    18541 gtgacattag acatctatgc acatattaca aataatgcac ctaagaaaac aggtcaagac
    18601 tttgctgata tgatggctca tcagtaaaag agctggtcaa aatctggtca tttctagtat
    18661 acaggcaaaa caaaaaagct agaaccctta tatatcaagg gttctagctc ctataacagg
    18721 tttacccaac actgccttcc atctcataac tgatcaatcg attgagttcg actgcatatt
    18781 ccattggaag ttctttggtg aatggttcaa caaagcccat gacgatcatt tcagttgctt
    18841 cagattcggt caggcctcgg ctcatcagat agtagagttg ttcttctgag atttttgaga
    18901 ctttggcttc gtgttctaaa gagacttggc tgttgtggat ctcattgaat gggatcgtat
    18961 cggatttcga ccattcgtcc atgatgatcg tgtcacattc gatatgagag atcgaacccg
    19021 cactgttttt ggcaaaggtt acttgtcctc gatagtttac ttctccgccg tcttttgaaa
    19081 ttgatttaga gacgatagag ctagatgtat ttggtgcatt gtggatcatt tttgcacccg
    19141 tatcttggat ttggtttgct cctgcaaagg cgatggagag catggtgcct cgtgcacctt
    19201 ttccatctaa gtaaacactt gggtatttca tcgttgtttt tgcacctagg tttccgtcga
    19261 tccattcaac ggttgcgcct tcgtatgctt ttgctcgttt ggttactaag ttgtacacat
    19321 tgtctgacca gttttggatc gttgtgtaac ggcaatacgc atcttttctt gtaaagattt
    19381 ctacgatggc ggcatgcagg ctgttacttg aataagtagg tgccgtacaa ccttcgacat
    19441 agtggatgct tgctccttcg tcaacaatga tcagcgtacg ttcgaattgc cccgtgtttt
    19501 ccgcattgat ccggaagtac gtttgtaatg gaacatcgac tttgacgcct tttggcacgt
    19561 aaataaaggt cccacctgac catacggctg aattcagcgc tgctaatttg ttgtcggttg
    19621 gcggtacaag ttttgaaaag tactctttga ataattcagg gtattctttc aaagcagagt
    19681 cagtgtcagt aaagacgatt cctaactttt cgaattcatc tttcatatta tggtacacga
    19741 cttctgattc atattgtgcg gaagcaccgg ctagataagc tcgttcagct tctggtatac
    19801 caatcttttc aaatgtatct ttgatttttt ctggtacgtc ttcccagtca cgagcgggtt
    19861 tgtcacttgg tttttggtaa tatttgatgg catcgaaatc gatgtctgaa aggtcggggc
    19921 cccatgtttg cattggcatt ttgttaaatg tttctagaga tttcagacgg aattcaagca
    19981 tccattctgg ttcgcctttg attcttgaca tctcacggat cacttcttct gtcaagcctt
    20041 ctcctgtact gaaaaccggt tcgacatcat cgtgaaaccc gaatttatat tcttctaatt
    20101 ctggtacgcc catgttctca ctcctcttcc ttgtggtaat gatggaaagc tgcggtacca
    20161 tcgttttcca ttgcttttcc tagtgctttc caggcaagtg tcgcacattt gatccgagcg
    20221 ggaaatttag ctacaccacc aagcatagca gcatcgccta attggtcggg atctttgact
    20281 tcttttcctt ggacaagctg tagaaagtct acagttaaat gttctgcttc ttctattgat
    20341 tttccgatga cggcgtctgt catcatcgag gcacttgccg tactgatgga gcagccactg
    20401 ccgtcaaagg cgatattttt gatgattccg tcttttacat cgacttgcaa ttcgatcaca
    20461 tctccacatg tggggttgtt caattcgatc ttgcttgtcg agtcagtcaa gcttccacga
    20521 tgatgcggat gggaagaatg atccaagatc acttgacggt ataaattgtc taatttagat
    20581 agtcccatgt tggaaaaact cctttgttgc catgattgct tcaactaaac ggtcggcgtc
    20641 ttgcgttgtg ttgtagaaat aaaagctcgc gcgagcagtc gctgatactt caaggtactt
    20701 caatagcggt tgcgcacaat gatgacctgc tctgacggct accccttcca tgtctaaagc
    20761 tgtcgcgaca tcatggggat gtaacccatc caaattgaat gcaacgactc ctgtccgaaa
    20821 attcgggtct tgtggaccat aaacagtcaa tccatcaatt gcttgtaatt ttggcaacac
    20881 ataagcggct agttcttctt catatcgatg gatattgtcc ataccgagtt tttgcaaata
    20941 atcgattgca tggcctaatg cgatagcgcc ggcgatattt ggtgtccctg cttcgaattt
    21001 ccaaggtagt tctttccatg tactgtcgta taattcgaca aaatcgatca tttcgccacc
    21061 aaattcaaca ggttccattt cttctaacca ttttcgttta ccatataaaa caccaatacc
    21121 tgttggtccg cacattttgt gtccgctgaa agcaaagaaa tcacagtcta gtgcttggac
    21181 atcaatggcc atgtgcgggg cagattgagc accatctact acgatgaccc cgccgttttc
    21241 atggacaagt tcagctaatt cttgaacagg attgaagaca cctaagacat tggatacatg
    21301 agcaatggag acgattttgg ttttgtccgt aatctttttc gctgcatcct ccatatccag
    21361 gaagccttct tctgttagtt cgatatattt caagacagca cctgttcgtt ttgccaactg
    21421 ctgccacggg ataacattag aatgatgttc catgtaagag agcactatct cgtctcctgc
    21481 tttgatatag cggtcgccga aggttctggc tacccaattc aagcttgtcg tcgtaccacg
    21541 agtaaacagc acttctgctg tttcttttgc atggatgaac gcccgtactt tttctcttgc
    21601 ttgttcataa tcatgggtcg cgcgttctgc taacgtatgg acaccgcgat gaacattggc
    21661 gttatcgtgt tcgtaatagt acgctaatgc ctggagtact tgttttggct tttgggtcgt
    21721 cgctgcatta tccagataaa ctaaaggttc atcattgacg tcttgaaata agataggaaa
    21781 atcattgcgt agctttttag catcgatcat ccattcaact tcccttcgat tacttcgact
    21841 aattctttcc gaacttgttc tacagggatt gctgttaaga cagaaccgag gaatcctcga
    21901 atcactaagc gctctgcttc ttctttatgc aatcctctac tcatcagata gtacatttct
    21961 tctggatcaa ctcgtcctac actggcagca tgtccagcag ttacttcgtt ttcatcgatc
    22021 aaaagaatag gattcgcatc accacgtgct ttatcagaaa gcatcaagac acgactttct
    22081 tgttgggcat ctgcaccttt tgcccctttc aagatgtggc cgataccatt aaaggtcaat
    22141 gttccttttt ctcggatcac tccgtgttgc aaaatatgcc cgatcgtatg aggggcttta
    22201 ttggttactc gtgtatcgat tccttggatt tgtctgcccg agctgattgc cacgattttg
    22261 acttctgcgt gtgcaccttc tcctgctaaa tctgagtcaa agtcagcgat cacatgaccg
    22321 tcattcatga caccaattgc ccagtcgaca gaagcatctc gtaaaatgtg cccacggcga
    22381 ttcatataag aagtaatatt ttctcctaat tgatctacgg ctgaatattt gatttttgaa
    22441 ccagctttag cgatgacttc cacgatgata ttacctgatg attttgctac ttgttctttg
    22501 gttgtctgga aacgttccag ataagaaaat tcactatgtt catctgccac gatcaacaca
    22561 tgcttgaaga agtgctcatc ggatgcgcca tcttgaatga ataaggactc gagtggttca
    22621 tcgatcacga catttttagg cacatacagg aaaataccgc tgttcataaa agctacatgg
    22681 agtgcagtca attggtcttc gttcatgttt actgcttttt tcatataata ttcttggact
    22741 agttctggat attcgatcat tgcagtgaac aagtcggtaa aaatgactcc tttttctgct
    22801 aattccacag gcagttgttc aaaaatcgtc catgaacctt gttggacaag cacaggattg
    22861 tctttcattg catcaaatgc cggaatcgtg cctgtttcac tgatggtttg tgtttctttc
    22921 acatcaaata acgaccagcg atggaatttc acccgatcga tatgcggcaa tggcagttca
    22981 tctgctttcg ctagtgcgtc ttgacggaag gttctcatcc aatcaggttc gcctttactt
    23041 aaagaaaaat catttaccgc gtcaagataa tcgagccagt ttttttcttt cattcttgct
    23101 cccccttacg cttcttcttc tttatagtcg attcctaatt ctttactgat accagcgtag
    23161 ccttctgctt ccagacgttt agctaggtca gcattccctg tcatgactac tcggccttcc
    23221 atcatgatgt gaacgacatc aggtgtgata tagttcaata atcgttgata gtgggtgatg
    23281 atcaatgcac cgaaattgtc tccgcgcatt tcatttactc ctttagcaac gactttcaaa
    23341 gcatcgatat ccagacctga atcgatttcg tctaaaattg caaatgtagg ttctaacatc
    23401 aacaattgca ggatctcatt ccgtttcttt tctccgccag aaaaaccttc gttcagataa
    23461 cgttcagcca tttcttctgg catatttagt aaagccattt tttcatctaa ctttttcaaa
    23521 aatgccataa cagaaatttt gtcgttttca tcacgtttcg cattgatcgc tgcacgcatg
    23581 aattccgcat tcgtgatacc tggtatctca cttggatatt gcatagctaa aaataagcca
    23641 agtcgcgcac gttcatctac ttccaagtct aaaacatttt tcccgtcaaa caaaatttcg
    23701 ccttctgtta cttcatagtt tggattgccc atgatagcgg cagataaggt agattttcct
    23761 gtgccgttag gtcccatgat ggcatggatt tctccggttt tcattgttag gtttacgcct
    23821 ttaaggattt ctttttcctc gatcgacaca tgtagatttt ttatttctaa gacagacatg
    23881 cattataccc ccaaattctc tttcgttctc actcatttta gactaattga gaatgaagcg
    23941 caatgacctt ccttactaaa atcaaatatc ttttatttat tattaaacaa ttttgatttc
    24001 taaattagaa taggtataaa aaagatgaga aataagccat tacagactta tttctcatct
    24061 tttttgttcc ataccttatt ttgcatcagc ctttactggt ttaacagagc cgccccattt
    24121 gtctgtgatc cattcttgga cttctttgcg gtgaagcact tcaatcagtt ttttgatttt
    24181 gtcactgtct tcatcacctt tacgagtcgc aataatattc acgtaaggag agttgtcctt
    24241 ttcaagcaat acggcatctt ctaatggatt caaccctgcg ccgtaagcga agtttgcgtt
    24301 gattgcaact aggtcacctt catcattttg gtacgtagaa gctaaaagtg atgggtcgat
    24361 cgtatggttg aacttcaagt tcttagggtt ttcttcaata tcatcgaatg ttgctgtttc
    24421 cagatctact ccatctttga ctttgactaa gccagcatct tgtagaattg tgatgatgcg
    24481 tccccagtct gattcagaat ttgaagtaat gattgttgca ccatctttta attcggaaac
    24541 atctttgact tttttagagt acaagcccat tggttcgatg tggacagcac cggcattgac
    24601 aaagtcatat cctttttctt tgatttcttt gttcaaataa ggaatatgct ggaagtaatt
    24661 ggcatcaatg tcgccttctt ccaaagattg gttcggtaat acatagtcat cgaatttgac
    24721 gatttcaaga tcgattcctt catctttcaa taatggtttc acatgttcca aaatctctgc
    24781 gtgcggtgtc ggtgaagcgc cgactttcaa tgtcgtgtcg tctgctttat ttgctgaatc
    24841 tttgctgtcg ctgttgttac cgcatgctgc taatcctaca gtcagcaaaa gcgctgctgc
    24901 aaatccaaaa attttctttt tcatatccta catcccccta tatcctatta tcttttgtct
    24961 gttttcttag ttaagaaatc accgattcct tgaacgataa atacgattag taaaataatt
    25021 aaagtcgcaa cgaatgtcac tgttagatta ccacgctggt agccttcctg ccaagcaaga
    25081 cctccaagtc cacctgcgcc gatcgcgccg gccatcgcag taaatccgat catcgagatc
    25141 gttgtaactg ttaatccaga aatcaatgcg ggaatacttt caggtatcaa gaccttccag
    25201 atgatttccc agtaacttgc tcccattgca tctgctgctt ccagtacacc agggctgact
    25261 tcacgaaaag caatctctac tagacgggca taaaatggtg ctgcggataa aacaagagct
    25321 ggaatggctg cttgcgcacc tagcatagtg cctacgattg ctttagtaaa tggaatgatc
    25381 aaaaccatca ggatcataaa tggcgttgac cggaaaatat tactgatgat cgatacgatt
    25441 ccataaatga ttcgagcata cggtttgttt ttgcgtccaa tagagtaaag gatcaatccc
    25501 aaaactaatc cgatgatcgt cactaagacc attgagatta atgtcatata tagtgtatca
    25561 aggatagcct gattcatcgt ttccgggttg atttgtttaa aatctagata tgtttctgaa
    25621 aagcttttat ccacgatgga tcacctcaat tcctactcct tgttctttga tgaacacttc
    25681 tgcttgatct aattctttgg tttcaccagt catcaagaca gtcaatgaac caaaggaacc
    25741 ttctttcact tgtttgatct ttccatacac aacgttgaca tcgatgttat agcgtcgaat
    25801 cgcttgggaa atgactggct cgttggcatt tctttcgcta aaggtcaaag ttgccaaacg
    25861 gccgcttgga ttttctttga tgagttcagc taatagttct gtcgaatcag cttctggttc
    25921 gatgtcttgt tgtacaaagc gtttggttac ttcttttttc ggttgtctga agacagtcaa
    25981 tacgtctcct tcttctacga ctttccccaa ctccatcaca gcgactttgt tgcagatttt
    26041 gcggatcaca ttcatttcat gtgtgatcaa gacgatggtc aaattcaatt tcttattgat
    26101 cgctaacaat aagtctaata cttcttctgt tgtttgcggg tccaatgcgc tagtagcctc
    26161 atcacaaagc aggatctcag gatcatttgc caatgctcgt gcgataccga cgcgttgttt
    26221 ctgtccgccg gaaagttgtg aaggatatgc ttgtccgcgt ccttctaacc cgactaaacg
    26281 caacagttct tctgcccgtt ctttacgttt gctctttggt acgccagcta attccagcgg
    26341 cagctggata ttttcaagta cagttcttga ccataacagg ttgaaatgct ggaagatcat
    26401 accgattttc tttcggaatg tacgtaattc tttgtttttt aattttgtaa tatcttgatc
    26461 gtttacgatc acttcgccgc tcgtgggaag ctcaagtcca ttcaggagac ggactaatgt
    26521 actttttcct gcgccagaat agccgacgat tccataaata tccccttttt caacgtttag
    26581 tgaaacgtca ttgacggctt ggatgtttcc gtttttccca ttgaatgtct ttttgacatt
    26641 cgttagttgg attaatgaca tgccattcac atctttctct tatgattttt caaacaaaaa
    26701 agccttcgcc ctcttgcctt aagcaaaagg acgaaagctt gaacttccgt gttaccacct
    26761 tgatttaccg ttacttcgca ataacagcct cttccagtac ttggacttag cttctttccg
    26821 tatactgtcg cgctgtaacg ggcactcccg tagtaagttt gcttacgctc gcttactctg
    26881 ctctaagacc atcttccatc tcattttcgt tacccatttt cagcaaacag ggctctcttc
    26941 aaacgtccgt gacatgtact cttcttttca aagctttctc gtttcatata ggattgctta
    27001 tgattatagc aactggaaga atcagtgtca acttttttca agtgattttc tgattttata
    27061 attcgtcaaa ttcttttgga tcaacatctt tgaaacttaa gatccacgca ttcatttcat
    27121 ctgaatcatt caagatattg atatcttcac cgattttatc attaacagaa gaaactgtac
    27181 ccgttagcgg gctggcaaat tcattgaccg ctttttccgc ttcaacttcg atcagaggtt
    27241 catctttttt tagcgattgt cctgcttttg gcagattggc aaacgtgatg tctccaagtt
    27301 cttcttgcgc ttcatttgtc aaaccaacac agtattcttt tccattaaat aagatccaaa
    27361 ggttgtcctt tttcttcaaa tgcttgcctg ccattttata tcagttctcc ttcataaatt
    27421 ttttcattga atccaattgc aacaagctgg tcctctcgta agatcagtgg tcgcttgatc
    27481 agcataccat ctgtcgataa gacttggctc gcttttgaag ccgtaaactc gcctacttga
    27541 tctttcagtc caagttcccg atatttcatc ccgcttgtgt tgaacaggcg acggattggt
    27601 acttgactcg tttctagcca agtctcaatc gttgaggcct tcggtacttc tttgatcata
    27661 tcaacggttt gataactaac gccatgctgg tctaaccacg ctttggcttt tttgcatgtt
    27721 gaacatttcg ggtaccagta aaaggtgtac aattttctta cctccttgaa gatcttgcag
    27781 gtgcttcatt ttggtatctc atcgataata tcaacccgat accgatcatg ttccctaaaa
    27841 tcgaggatcc tccctgacta atgaaaggca atgggatccc tgttaaagga agtaatccga
    27901 tatttgctcc aatattctca aagacatgga acagcagcat catgatcaaa ccgcttgcga
    27961 tataggcgta aaattcatta ttcgtatcga aacacacccg aatcatacga tagattaaaa
    28021 taaaatacaa gaaaatgacg aatgcaccac cgataaagcc gaagttttca ccgataacgg
    28081 taaaaatcat atcggactca cgaacaggca catagatatt gcttacgttg aatcctttac
    28141 caaacaagcc accggtacca attgccaaaa gtccttgggc cggctgataa ctcattcctg
    28201 acgtatcatg gaaagggtcc agccatgaat caatacgtgc aaactgataa gatttgaatc
    28261 caactttata aagcaagtct cttccgcctt cagtcgtaac caagaagatc gttcctgctc
    28321 caacgactac tgccaagatt actacaggtg cgatgatgcg ccaagagatt ccagacatca
    28381 ggaaaatccc gccaaagatc gcaagaaaga caagcatcgt tccaaagtca tcttgggcca
    28441 tgatcaatgc taaaaccggt attgtcacga tcaagagctt cccaatcagc caaaagtccg
    28501 atttcatcgt ccgttctttt acttgcgtat tatgtttagt tacgatcagt gccatcatca
    28561 agatgtacgc aatcttcatc aactctgctg gctggaaagt aaacgcaccg aatcgaaacc
    28621 agtttttaga ccctgttgaa gcagctaaat tctgatcata gaatttgagc aagagtccca
    28681 ttaccactaa ccccaaacca taaaggtaag gagtcagttt ccagatccat ttggaattca
    28741 aatgcatgat gacaacgatt gctgccgctc caactaaata ccagacaact tgcatcccta
    28801 cgcctttcaa gactgctgga ttgttcggat catggctaag tgctacatat aatgagagca
    28861 gaccaatcaa acataacaga aagactggta aaatcacgcc ataatcaata cgattatcgt
    28921 tcgaagcttt taattttttt tccatgtttc atcccttttc ttaaaaacca tactgctgat
    28981 tattatagcg gtccagttag ataatggaaa gtctttccat agacatttgg gaaaaattaa
    29041 ctttccgttt gtgcagttgc ttgagcaaat gcagtacaaa agcctactgc ttatctttag
    29101 tgtatcatag aacttttttt gatacatgca attatctacc agctgagatt ctcctcgctg
    29161 tgagaagttt ctgtgctttc attgtatcga aggatctctt cggcaatctc tagcagctga
    29221 tcagaaacca gattggttcg ttggatgaaa taaaaatatc gatggaaatt caacggagtc
    29281 attggcacat tttctggaac agagcgctta gacaaaatcg agcgaccgat tccttccttc
    29341 agcaacgcaa caattacatc gttacttttc acgatcatct tcttatcttg gatattcatc
    29401 tcttcaaaat aacgctgcgt gtaatgaaaa acaccagagt ttgcttctct tacaagccac
    29461 aatttgcttt ttgggtcccc agcgatgact agctggtctt ccatcaaaga gtatctttgt
    29521 acgccggaag tctctaatgg cttttcgata aaaccaaaat cggattcatg tttcgctact
    29581 tgttctaatg attcatgaga attcgtgatt tcgatctcaa aatgcacttt aggaaacctt
    29641 ttgatcagat gtttcatcaa ctgaggcaaa agatagacag caaaggtgtg ggaagcaacg
    29701 atccgacata cttcttcttc attttgttca ttgagcaatt tttgctgagt ttcttcccat
    29761 tcttctaaca aaaccagtgt ccgttcatat aataagtcgg cttgcttagt tggaagcatc
    29821 tcttttcttc cgttacgttg aaaaagatcg atattcaatt cccgctccaa ctgtttgatt
    29881 tgattcgaga ctgctggttg agataaaaac agtacttctg ctgctctgga aaaatttctt
    29941 gtttcatata ctgctcgaaa agtcgttaat aacttaaaca tggcaccact tccataactt
    30001 tttatgataa ttactataaa aactatttga tttaaatata caaaaaacca tctataatag
    30061 aaatgtacaa caaaacagtt gctgatccaa ctagtaactt agatgaagac aatcagctgt
    30121 ctgcttgctc gtaatcatac attcctattc attttacaat gtattcgttc atcttggtac
    30181 taaaatctat taaataaaaa ggagaatatt atatatgaag aactttttga aaattattcc
    30241 gatcccgatc tgcgggttga tccttgggtt agcttccctt ggaaatctgt ttaaagacta
    30301 tcaccatgtt gctttaggca atctaactgg cggtatctct atgattctta tgatattcat
    30361 ttttgttaaa ttgtttatct tattcgaaca cacgaaacaa acgttgcatg atccaatcat
    30421 cgcctccgtt tcaccaactt ttacgatgtc actcatggtt atttgtactt attttgtttc
    30481 atatgaaagt attgcaccaa ttgtcaaatt catttggctg tgcgctgtta tcttccaagt
    30541 gatccttgtc ttttatttca attatcacca tgttatcaaa gctgatcttt caattgaagc
    30601 aatttatcca agctggttta tcatttacgt tggatttggg gtcatcactg taactgccgg
    30661 gaacttcttc ccacttattg gaaaaatttt cttttggatt tcattgatct gctatgcgtt
    30721 cttattgccg atcatcatca aacgtgtctt tttcgtaaga aatatggctc atccgacacg
    30781 tccgctgatt acgatcgttg cagcacctgg ttctctttgc ctaacaggtt atcttaaaaa
    30841 ctttgctgaa cctaattttt atcttgtcgt agcattgttc gctttatctc aagtattgta
    30901 tttcgttgtg ctgtctatgc ttccacgttt gttcaagctt cgtttttatc caagctatgc
    30961 agcctttaca ttcccgcttg ttatctctgc aacagctata tttactactg ttcattactt
    31021 caacagttta ggtatctata gtagcgtatt ggatttctta aaagttgcgg aatgtctgtt
    31081 tgcaagtgta gttgtcttct acgttcttgc tcattacgta cgtttcttaa ttaaagaaca
    31141 tagcaaacat ggaaaatttg tttctaaaaa aatcgcttaa taatcgattc aataggttgt
    31201 gaaaaagctg ttctgcatca agtaataaga acggccaaac aaaaacagct gttcatgttt
    31261 ttgtactggt tgggcttatt accgcagatg caagcttttg aacaccgttt accaagaggt
    31321 cgtaa
//
LOCUS       DACEUZ010000015        30947 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.015, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000015 DACEUZ010000000
VERSION     DACEUZ010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30947
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.015"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            82..1395
                     /locus_tag="IXG44_000630"
     CDS             82..1395
                     /locus_tag="IXG44_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAQ1657369.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEIAKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            1392..2111
                     /locus_tag="IXG44_000631"
     CDS             1392..2111
                     /locus_tag="IXG44_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HAQ1657370.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            2239..2790
                     /gene="raiA"
                     /locus_tag="IXG44_000632"
     CDS             2239..2790
                     /gene="raiA"
                     /locus_tag="IXG44_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HAQ1657371.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADATALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            3012..5546
                     /gene="secA"
                     /locus_tag="IXG44_000633"
     CDS             3012..5546
                     /gene="secA"
                     /locus_tag="IXG44_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HAQ1657372.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPHHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKSFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            5672..6773
                     /gene="prfB"
                     /locus_tag="IXG44_000634"
     CDS             join(5672..5734,5736..6773)
                     /gene="prfB"
                     /locus_tag="IXG44_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078989099.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HAQ1657373.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRGLFDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            6878..7564
                     /gene="ftsE"
                     /locus_tag="IXG44_000635"
     CDS             6878..7564
                     /gene="ftsE"
                     /locus_tag="IXG44_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HAQ1657374.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            7548..8441
                     /locus_tag="IXG44_000636"
     CDS             7548..8441
                     /locus_tag="IXG44_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1657375.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            8593..10041
                     /gene="cls"
                     /locus_tag="IXG44_000637"
     CDS             8593..10041
                     /gene="cls"
                     /locus_tag="IXG44_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAQ1657376.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWVLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGGKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            10378..11235
                     /locus_tag="IXG44_000638"
     CDS             10378..11235
                     /locus_tag="IXG44_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HAQ1657377.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            11350..12270
                     /gene="pstC"
                     /locus_tag="IXG44_000639"
     CDS             11350..12270
                     /gene="pstC"
                     /locus_tag="IXG44_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HAQ1657378.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            12270..13154
                     /gene="pstA"
                     /locus_tag="IXG44_000640"
     CDS             12270..13154
                     /gene="pstA"
                     /locus_tag="IXG44_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HAQ1657379.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            13169..13975
                     /gene="pstB"
                     /locus_tag="IXG44_000641"
     CDS             13169..13975
                     /gene="pstB"
                     /locus_tag="IXG44_000641"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAQ1657380.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            13991..14749
                     /locus_tag="IXG44_000642"
     CDS             13991..14749
                     /locus_tag="IXG44_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAQ1657381.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            14762..15439
                     /gene="phoU"
                     /locus_tag="IXG44_000643"
     CDS             14762..15439
                     /gene="phoU"
                     /locus_tag="IXG44_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HAQ1657382.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            15677..16708
                     /locus_tag="IXG44_000644"
     CDS             15677..16708
                     /locus_tag="IXG44_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAQ1657383.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGKLMNTDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGW
                     LFFFTSIFIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISILFVV
                     IGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTRP
                     YRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSM
                     TNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLF
                     FLTVRRLKR"
     gene            16705..17472
                     /locus_tag="IXG44_000645"
     CDS             16705..17472
                     /locus_tag="IXG44_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAQ1657384.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            17572..19140
                     /gene="liaX"
                     /locus_tag="IXG44_000646"
     CDS             17572..19140
                     /gene="liaX"
                     /locus_tag="IXG44_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HAQ1657385.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            19440..19757
                     /locus_tag="IXG44_000647"
     CDS             19440..19757
                     /locus_tag="IXG44_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HAQ1657386.1"
                     /translation="MKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVAF
                     GSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDEW
                     SDF"
     gene            19757..20107
                     /locus_tag="IXG44_000648"
     CDS             19757..20107
                     /locus_tag="IXG44_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HAQ1657387.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            20274..21212
                     /locus_tag="IXG44_000649"
     CDS             20274..21212
                     /locus_tag="IXG44_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HAQ1657388.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            21227..22057
                     /locus_tag="IXG44_000650"
     CDS             21227..22057
                     /locus_tag="IXG44_000650"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HAQ1657389.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            22101..23126
                     /locus_tag="IXG44_000651"
     CDS             22101..23126
                     /locus_tag="IXG44_000651"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAQ1657390.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHHASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            23141..24079
                     /gene="galU"
                     /locus_tag="IXG44_000652"
     CDS             23141..24079
                     /gene="galU"
                     /locus_tag="IXG44_000652"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAQ1657391.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYIMSLGKTLEAEGSKINHNPKGITEIKEEKKK"
     gene            24204..24644
                     /locus_tag="IXG44_000653"
     CDS             24204..24644
                     /locus_tag="IXG44_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HAQ1657392.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTQDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIVTKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            24647..25249
                     /locus_tag="IXG44_000654"
     CDS             24647..25249
                     /locus_tag="IXG44_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAQ1657393.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSSELTGIAKEKAQGLSQQAGDLAGNVKQKVSESAEEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDLKKPADELGSIAKDTSEDIMIDVKDAADKAGETVNEG
                     MSEAKPVADDTKEMLKMAKEDAKEAKKEMKDTMTDSTTNH"
     gene            complement(25312..26415)
                     /locus_tag="IXG44_000655"
     CDS             complement(25312..26415)
                     /locus_tag="IXG44_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAQ1657394.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVDRFDAISDILTGTDSYTDLTSIIQRLQLIKTVEEKDKLIEAGKWADVAF
                     EIGFKTVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGSTKVSP
                     NELVLFDLGVIWNGYCSDATRTVAYQKPTEFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLE"
     gene            26625..27626
                     /gene="ccpA"
                     /locus_tag="IXG44_000656"
     CDS             26625..27626
                     /gene="ccpA"
                     /locus_tag="IXG44_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HAQ1657395.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            27981..28376
                     /locus_tag="IXG44_000657"
     CDS             27981..28376
                     /locus_tag="IXG44_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1657396.1"
                     /translation="MPKKRRKTIVIILTILTIGIGGKFYMDKREMQRQKDLLAVEKQS
                     VKVLKNTFADIKEVKIEKSARNEMTGSYRIVILMTNKQDQSIYFSYSFWKERNEIGSY
                     GIVDEKKQKEGNTLNKVKVTYSNGNEESI"
     gene            28376..28627
                     /locus_tag="IXG44_000658"
     CDS             28376..28627
                     /locus_tag="IXG44_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1657397.1"
                     /translation="MITDKQYSELSDAVYWLDPKHRDYVPEMQENLSFKINGTMYKIL
                     KIKNSFDGMQAMAVAPIVHSKLEKNFKNKKIPANFRVLK"
     gene            28670..29065
                     /locus_tag="IXG44_000659"
     CDS             28670..29065
                     /locus_tag="IXG44_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1657398.1"
                     /translation="MSRKIRKLSLAILLIVILGIGGKVYMDKREAQRQKDLLAVEKQS
                     VKVLKNTFADIREVKVEEFKKNPVTGSYGALVTMTNNEGKSVYFDYSFWKERNELGGF
                     GIENRAVQKTGVTIKRVKVIFSNGQEELV"
     gene            29062..30318
                     /locus_tag="IXG44_000660"
     CDS             29062..30318
                     /locus_tag="IXG44_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HAQ1657399.1"
                     /translation="MTINDKHYNDISEKVYWLDPKYPRYNEGYKKNSVKEFAGMEFQI
                     LQIKDSLDGMQAMSVAPIKNGKVDLSQVVIAFAGTNLSDWKDLETDARSIMNLYGNRP
                     GASFSEKATTNKILSVSGLKTAGQINSAIAFANEVKKEYGEAAISTTGHSLGGFLALY
                     VAAEKQWKNVGFNGPDPYDLLSPEAKKWVEENPGMLTNYRNHADFIGNFGGNGTGAEV
                     RISMETAFLNLLASHSLTSWKFDENGKLIIPENENNVKARKQQKENQATQLFSYQLQE
                     LNNIRKKLRLSGGQLTVNEQIYLDDTQARLVVEKLSAKMKSAMEEVIRINQRGIFELE
                     KKWQEGLRQARVVAPSLTEQEIIEALASVGATKENLVDSAKEVYLLRINKARRMGEAF
                     DTLAKEIQDKINEIVARDRELAQQFN"
     gene            30330..30653
                     /locus_tag="IXG44_000661"
     CDS             30330..30653
                     /locus_tag="IXG44_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1657400.1"
                     /translation="MKLEELERDYRSERRQLEAQEDELIHFKNQGLRLMEQEEQRLHH
                     VLKNLALDNEPLFQARRSYSNYQEHYQQALQVERRKLYQQQDELERSYRQKIRQITME
                     KSSYE"
     gene            30646..30912
                     /locus_tag="IXG44_000662"
     CDS             30646..30912
                     /locus_tag="IXG44_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1657401.1"
                     /translation="MNEFLMQLLKELEKNLHRSNVRSVGMGISQQIWAEKRLTDQNYQ
                     MTAEEAAQEFSNRHVHLKGSVKGQFAEKLTELFQRQEKKLKQFR"
BASE COUNT        10412 a         5327 c         6670 g         8538 t
ORIGIN      
        1 aatagaacag tataactatc gaacaagcga tccaaaaaat agatcgcttg ttttttcgta
       61 tattttttta gggcgaataa tatgaatcat ttttggggta gaaggcttct gacgagagat
      121 attaaagcaa tagactgtga ggaggtaaat ttaccaaatt atgagaagat cgcagcagtg
      181 gaacgtttgg gcaactggat tctttgtcat cgctgtggaa tgaggacgtc cgtcattgat
      241 ggacagcttg cagattacgg ttatttttgt acacattgtc tatatttagg gcgatgcgac
      301 actcaacaag atttatattt gtttgatcag cctgaagcaa aacctcgaga agtcgtcttt
      361 ttttggacag gacagcttac tgaaaaacaa acggaaatcg caaagagaat actttgccat
      421 tcagaaaaaa gacatcatct tatttgggct gtaacaggtg cagggaaaac agagatgctt
      481 tatcctattt tagtggaaac tttaaaagct ggaggaagag tagcgatttg tacaccgcga
      541 gtcgatgttt gtaatgaatt gtttttacgc tatcgttatg tttttccaga agaaaaaatc
      601 acgttgctcc atgggaatac agcaacggct tataccttta cttcttttgt tatctgtacg
      661 attcatcagc tataccgttt ttatcggtcc tttgatttaa tagtgatcga tgagatcgat
      721 gcgtttcctt ataacgggga tgcaggatta gctcatgctg tgcaaacagc gattaaacca
      781 gacggacgat tcatctactt aagtgccaca cctgataaaa agttactaaa ggaaatcaag
      841 cagacgtttg acatccacaa acttgctttg cgctttcatc ggcgtttact tcctgaaccc
      901 gtcttggttt tcatgaataa ttggcgacaa aaatgcaaga acaagaagaa gataaaatct
      961 ttggtgcgga tcattcatca attaattaaa gaaaaccata tccttttatt ttgtccaagt
     1021 atcacgatga tgcatcgttt gaaagccaca cttcaagagg ctcttccgag atatgagatc
     1081 acagatgttt cttcaagaga caatcagcgg gcggaaaaag tccagaaaat gcgagaagca
     1141 aaatatgaca tactgttaac cacgatgatc cttgaacgtg gagtcacatt tgaaagaata
     1201 tccgttatcg ttttaggagc ggatcatccg gtttttacta aatcttcttt ggtacaaatc
     1261 gcaggaagag ccgatcgtaa agggccttat acgaacagtc aagtatactt tttctatgaa
     1321 gaaaaaactg cagctattcg aaaagcttgt caggaaatca aagaaatgaa tgaagaaggg
     1381 aagaagatgc tgtgaggtgc agttgctgca gtaaagatat cgtccaaaat ttaacattca
     1441 gcgagattat tggtgtttcg aaatacagat tacctcagac ttgttcagtt tgtcgcgcac
     1501 aatttcattt attggaaaca agaggctgtt ctggctgtca aaaaccaatc catttcaatc
     1561 tagagagtac gaaaaagaaa gtaaagtcta ttcccgttta ctgcagtgac tgtcaagaat
     1621 ggaagaagaa atatccgacc tatgattttc agcaccgtgc cttttttgtg tacgatgaaa
     1681 atatgaaggc ctggctcaat caatacaagt ttatgggaga tatccgacta gcaggcacct
     1741 ttgcatataa atggacgcag ataaaaaaag aatacccgac atacatctat tgtccaatac
     1801 ctttatctga aaaaaggcga acagaaagag gattcaatca agtaagcgag atgttgaaag
     1861 cagcaagaat tccttttcag atgctactca aaagagagaa gcatctgctg gctcaagcaa
     1921 aaaacacgcg aaaggaaaga ttggagatgc ctcaaccttt tgaacttgca gtcacctttt
     1981 ctaagataca aaataaaaaa atattgatta ttgatgatgt ctatacaaca ggacagactc
     2041 ttttccacgc agcagattgt ttgcaggtcg cacagccgaa aatcatccga accatttctt
     2101 tagcaagatg aattagatgt ggaaatttga tagcgattta atttgcaaat aacctagagt
     2161 tcgatataat agaattaaga agagggaaag agtggtcctt ctccttcttg attgtggtag
     2221 acgaaagggg taatttttat gtttagatat aatgtacgcg gcgaaaatat cgaagtaact
     2281 gaagcgattc gcgactacgt tgagaaaaaa gtaggcaaac tagaacgcta tttcagtgac
     2341 gcacccgatg caacggcgca tgtgaactta aaagtttata cagaaaaaac agcaaaagtg
     2401 gaagtaacga tcccattacc ttatcttgta ttacgtgccg aagaaacttc tccagattta
     2461 tacgcaagta tcgatttggt tgttgacaaa ttggaacgcc aaatccgcaa attcaaaaca
     2521 aaaattaatc gtaaatcccg tgaaacgggc atgaatactg cagatgctac tgctttattc
     2581 aatgaagagg acgaaaacga aaatgattct gaattagata tcgtacgtac gaaacgcctt
     2641 tcattgaaac caatggatag tgaagaagca gtcttacaaa tgaacatgct gggacataac
     2701 ttctttattt ttgaagatgc agaaacaaat ggtacaagta tcgtttaccg ccgtaaagat
     2761 ggaaaatacg gactgattga aacagactga tcagccttac ttaaataaca acgaaaactt
     2821 gcagcagcta ctgacagctg ccgcaagttt ttttgagttt cctcctcttt tagatgaaat
     2881 atgaataaat tatgaagttc ttactttaat acgcttaaga tatttcaatt atctataact
     2941 aatgataaaa tgaaaagtaa agtctacata gtctggataa tacacagaag gaaaaggaga
     3001 ttacacaacg catggccaat ttgattcgtt cacttatcga aaacgataag aaagaactaa
     3061 aacgtttaga tagcattgca aaaaaagttg aagcatacgc tgaccaaatg gcagcattaa
     3121 ctgatgaaca attacaggca aaaactgctg aattcaaggg cagataccaa aaaggagaaa
     3181 cattagatca attacttccg gaagcttttg cagtcgtccg agaagcagca aaacgtgtat
     3241 tggggttata tccacaccac gtacaattga tgggtggaat cgtgcttcac gatggaaata
     3301 tccctgaaat gcgtactggt gaaggtaaaa ccttgaccgc aacgatgcca gtatatctaa
     3361 atgctctgac tggcgaaggg gtacacgtag ttaccgtcaa tgaatattta gcaacccgtg
     3421 actcgacgga aatgggcgaa ctttataatt tcttaggatt aacagtgggg ttgaatatca
     3481 actcaaaatc ttcggatgaa aaacgcgagg cttataattg tgatatcact tatagtacga
     3541 ataatgaatt aggatttgac tacttgagag acaatatggt cgtttaccgc catcaaatgg
     3601 tacaacgtcc gctgaattat gccattgtcg atgaagttga ctcaatcttg attgatgaag
     3661 cgagaacgcc gctgatcatt tcagggcaag cagaaaaatc cacagcttta tatacaagag
     3721 cagacaactt cgtgaaacgt ttgaaagaag aagaagatta taaaatcgat gtacaatcaa
     3781 aaacaatcag tttaaccgaa gcagggatcg aaaaagcaga agaaacattc ggtttggata
     3841 acttatacga cattgaaaat actgcattga cccatcactt ggatcaagca ttgagagcca
     3901 actatatcat gcttttagat atcgattatg ttgtccaaga cggaaaagta ctgattgttg
     3961 accagttcac tggtcggatc atggatggac gccgttattc agatggactt caccaagcaa
     4021 tcgaagctaa agaaggcgta gagatcgaag acgaaacgaa aaccatggcg acgatcactt
     4081 tccagaacta tttccgtatg tacaagaaac ttgcgggaat gactggtaca gctaaaacag
     4141 aagaagaaga attcagagaa atttataaca tccaagtata ccagattccg acaaatcgtc
     4201 cggttatccg tgatgatcgt ccagatttgc tttacccaac attacaaagc aagttccacg
     4261 ctgtcgtgca agatatcaaa gatcgctacc gcaaaggtca acctgtgtta gtcggaactg
     4321 ttgcggttga aacatctgag ttattatcag atatgctgaa tcaagaacgt attcctcatg
     4381 aagtgctgaa tgcgaaaaat cactttaaag aagcagagat catcatgaat gctggtcaaa
     4441 aaggtgctgt gacgattgct accaacatgg ctggtcgggg aacagatatc aagttaggac
     4501 ttggggtaag agaacttggt ggtctagcag ttatcgggac agaacgacat gaatctcgtc
     4561 gtatcgataa ccagcttcga ggacgtgcag gacgtcaagg agaccctggt atgtcacagt
     4621 tctacttgtc attggaagat gatttgatga aacgattcgg ttcagaacga atcaaatctt
     4681 tcttagatcg gatgaagatc ggtgaggaag atgctgttat ccaaagcaag atgctgacga
     4741 aacaagtaga atctgcacaa aaacgtgtag aaggaaataa ctatgacaca cgtaaaaacg
     4801 tcttacaata cgatgatgtg atgcgtgaac aacgtgaagt aatctataaa cagcgacaag
     4861 aagtaatcat ggaagaaaaa agcttatcca agccactaat gaacatggtc aaacggacaa
     4921 tcagtcgtgt agtagatgcg catacacaat tagagaaaga aaattggaat ttggaaagta
     4981 tcatcgattt tgccggcagt actcttgtcc atgaagatag tattactctt gcagatatcg
     5041 aaggaaaaac agctcaggag atcaaagagt atctaaacga acgagcacaa gaagtattta
     5101 ataccaaagc ggctcaacta aatggtccag agcaactgtt ggaatttgaa aaagtcgtga
     5161 tcttgcgtgt tgtggactct aaatggacag accatattga tgcaatggat caattacgtc
     5221 agtcaatcgg cttacgtgct tatggacaga acaatccatt agtcgaatat caaacagaag
     5281 gctataagat gtttgaagat atggtaggcg ccatcgagta tgaagtcact cgaatcttta
     5341 tgaaagctga gatccgtcaa aacgtacagc gtgaacaggt ttctcaagga acagcttctc
     5401 actcagaaga cggagatgcc aaagaaaata gtaatactag cgcgaaaaaa cagccaatcc
     5461 atgtgaacaa ggtcggaaga aatgatcctt gtccttgtgg aagcgggaaa aaatataaga
     5521 actgtcatgg aaaaaatgca aactaatttc tgacagcagg attggtgaga caaattcttt
     5581 ttttaagaat tcgtctcacc ttctttctgt tatttgctga ttctgttaga ctagatataa
     5641 tgaaaaaagt atagtgaaag aaggaaaaaa catggaaata tccgaaattc gaaatctcct
     5701 gacagaaatg caggaaaagg tcacaagctt caggaggtct ctttgactta gaccagctgg
     5761 aagaagacat tgcggaagca gaagcaaaaa tggctgaacc tggtttttgg gatgatagcg
     5821 aagctgctca aaaagtgatc agtgaaaaca atgcaaataa agaaacttat gatcaattcc
     5881 attctcttgc tgaagaactg gaagaaatcg aattgatggt ggagatgttg caggaagaac
     5941 ctgatgcagc catgcaagaa gaagcagaaa cacgtatcaa tgcgctagat gaaaaaatga
     6001 agacatatga attatctatg ttgttagatg gtccttatga tcgaaacaac gccatcgttg
     6061 aattgcatcc aggggctggc ggaacggagt cccaagattg gggcagtatg cttttacgta
     6121 tgtatacacg ctgggcagag cagcatgggt atcaagtaga gacactagat tatcaagcag
     6181 gtgatgaagc aggaatcaag agtgtgaccc tactgatcaa aggacacaac gcatttggtt
     6241 atctgaaatc agaaaaaggt gttcatcgcc ttgtacgtat ctcgcctttt gactcagcga
     6301 aacgacgtca tacttctttt tgttcggtag atgtgatgcc agagcttgat gaaacgatcg
     6361 atatagaaat caatccagat gatttgaaaa tcgatacgta ccgagcaagt ggtgcaggtg
     6421 gtcagcacat caataaaaca gaatctgctg tacggatcac tcacttgcct acaggaacag
     6481 tagtagctag tcaggcacaa cgttcacaat tgaaaaaccg tgagcaagcg atggggatgt
     6541 taaaagctaa gctttatcaa ttagaagtgg aaaagaaaga acaagaagca gctgcacttc
     6601 gaggagaaca aaaagaaatc ggctggggtt cacagatccg ttcttatgtt ttccatccat
     6661 attctatggt aaaagaccat cggacaaacg tagagacagg aaatgtccaa gcagttatgg
     6721 acggtgacct tgatttattt attgacggct atttaaaaat gcatctacaa taagaaccca
     6781 ttgttaaatt gaaataaaat tgtaagattt tgcaagcatg ttgaaacatt gccatgctat
     6841 aatagctaaa gactgaaaaa gatatggaga gaacgccatg attgaaatga aggatgtaat
     6901 gaagaagtac tcaaatggta cgaccgctat ccgcaatctc tcaattgtca tcgaacaagg
     6961 agagtttgtc tatgtggttg gtccgtctgg agccggtaaa tctacattca tcaaattgat
     7021 gtatcgagaa gaaaaagctt caaaaggaac tttgaacgtc gcaggtcatg atctaatagc
     7081 aatcaaaaac cgcgaagttc cttatctacg cagagaaatc ggcgtggtgt tccaagacta
     7141 taaattatta ccaagaaaaa cagtatatga aaatgttgcg tacgctatgc aagttatcgg
     7201 cagaaaacct cgggatatca aaaaacgagt aatggaagtg ctggaccttg ttggtttgaa
     7261 acacaaagtt cgagtctttc ctagcgaatt atccggtggg gaacagcaac gtgtctctat
     7321 tgctcgggca atagtgaata cgcctaaagt acttatcgcg gatgaaccga caggaaatct
     7381 ggatccagaa aactcatggg aaatcatgaa attattagat cggatcaatg cccaaggaac
     7441 tactgtcgtg atggctacac ataacagtac gatcgtaaat acgattcggc accgagtaat
     7501 tgctatcgaa aacggccgca ttattcgaga tcaagcggaa ggggaatatg gatacgatga
     7561 ttagaacctt tttccgccat ttattagaaa gtatcaagag tttgaaacgt aacggctgga
     7621 tgacagttgc ttcagtcagt gcggtaacga tcacattgac tttagtcggt gtattcatgg
     7681 cagttatcat gaacgcaact aaattagctc aagatataga aggaaacgtt gatgtctctg
     7741 tattcgtaga tatcggtaca aaagacgaag agatgaaaaa gctagaaaaa gaattgaatg
     7801 acttagatca tgttaaaaaa gttgaatttt caagcaaaga acaggaattg aaaaaaatcc
     7861 aaaacgaaat gggtgattcc tggaaattgt ttgaaggtga caataacccg ttatatgatg
     7921 tgtatgtagt aagtgctact gatccttctt atacaaaaca agtagcagaa gaagcagcag
     7981 atttgaagaa tgtttctcgt tctgattacg gtggagcttc ctcagatcgg attttccaaa
     8041 tcgctggtgc agttcggaca tggggattag ttgcagcggc attgctgctg ttcgtagcga
     8101 tgtttctcat ttcgaacacg attcggatca cgattttgtc tagacagcgt gaaattcaaa
     8161 tcatgcgttt agtcggtgca aaaaatggat atattcgctg gccgtttttc cttgaaggtg
     8221 gctggatcgg tctgatcggt gcgattcttc cgattttgat catcacattt ggttatgcat
     8281 ggtttttcca attgatcaat ccatcattat tacgttccca ttattcattg attcatccac
     8341 aaaatatcgt ttggaaaatc aatcttttaa tggtcggcat tggtgtgatc attggttcac
     8401 ttggctcgat catttctatg agacgtttct tgaaattcta ataccacaat ataaataaaa
     8461 tgagaaagtt ggacagtttc gttccagctt tcttttttta ttggctagga gaatagaagg
     8521 aagcggcttt tttcccttta ctttaaaaaa caacttgggt attcttaagt tggtagaaga
     8581 agaggtgaag ccgtgaaaat tatcgttgac aattttttta cgatcctttt gattatgaat
     8641 attttgcttt cctttatcat cgtttttcga gaaagaaagg aaactgctca aacatgggca
     8701 tgggtgctcg ttttgatgtt cattccagtt gtaggattta ttttatatat ttttttagga
     8761 agagggattt ccaaagagaa aatctttgat ttgaagatcc aagggaaaat cggtaagaac
     8821 ctggaaattg aagaagacaa acaggcattg atgcgagggc tttatcctca tccgcctact
     8881 ggacaagtag atgtgaaaca attgatctat atgctcacgg tatttgaaag tacgctctat
     8941 acgactaaaa atgaaatcac tttatttaca gatggtcgtg aaaaattcga tgcgctgatt
     9001 gaagatatca agcaggcaga agaccatatc cacttccagt attatattta tcgcagtgat
     9061 gcattaggtg aagaagttcg agatgcattg acagaggcgg caagacgagg gggaaaagtc
     9121 agagtccttc ttgatgcatg gggttccaca caagtttctt ccagcttttt tcagaatttg
     9181 aaaaaagcag gcggagaagt cgcatttttc tttcctctat ttgttccgta tattaatcct
     9241 cggatcaatt atcgcaatca tcgtaaaatc gtagttattg atgggaaaat cggatataca
     9301 ggtgggttca atgttggaaa tgaatatcta ggattggtaa agaaatttgg ttattggcga
     9361 gacaatcatt tgcgtattta cggagaagcg gtgtatacct tgcaaaaccg ttttttgatg
     9421 gactggaatt ctcagcatac aaaagaagta ggctattctc ccaaattttt tccgtccatt
     9481 cactctactg gtgatatagc tgttcaaatc gtgacaagcg gaccagatac cgaacatgaa
     9541 caaatcaaaa tgacttattt gaaaatgatt tcgttagcca aacgggagat attgatccaa
     9601 acgccttatt acataccgga tggatcgatt catgaagcac tgaagttggc tcttctttca
     9661 ggcgtaaaag ttcatatcca aatacctaac aaaccagatc atcttctcgt ttactgggca
     9721 acgtattctt tcgctgcaga gctaatcgag tatggagcaa ggatcgaaac atatgagaat
     9781 ggatttattc atgcaaagac gatgattatc gatggtggca ttgtttcagt tggctcagca
     9841 aatatcgatg ttcgttcatt tcgcttggac tttgaggtca atacactggt ttatgatgaa
     9901 cgaatggctt cacgtgtaag aaaagctttt tttgaagact cgaaaatttc tacgcatctc
     9961 acaaaagaaa tgtatgaaaa ccgaggaata atcatcaaga tgaaagaagg attagcaaga
    10021 ttgatttcgc cgctccttta aaacataaaa aggacatgag aaccatcaac agatggagat
    10081 ctcgtgtcct tttctaacgg ggccatttct gagagtacgt cagaaatgga gaaagcgtct
    10141 cgcatgagcg aaattaactt tggggtatct cttgtttttt ggggaaagca agaaataagc
    10201 taatatatgc tattaaaaga gacacatttc aaagccagta tagcatagaa acttttaatg
    10261 agatagtttt ttttacaatt atgagaattt attttgttat ttaatgattt tgattcgttt
    10321 tcatcaagta atgaaaatga taaaataaac atactttagg attaaaggag atttctaatg
    10381 aaaaaactta aattattctt accaataata ggattagcta ttcttttatc aggctgttca
    10441 aagtggattg atcgaggaga atccattacg gctgttggct cttctgcttt acagccttta
    10501 gtagaaacag ttgctgaaac ttaccagaca gaaaatcctg gaaaattcat caatgtacaa
    10561 gggggcggaa gcggaactgg tttgtctcaa gtacagtcag gagcagtcga catcggaaac
    10621 tctgatttgt ttgcggaaga aaaatcgggg atcgatgcct cagcattagt cgatcatcga
    10681 gtagcagttg ttgggatcac tcctattgtg aacaaggata ttggcgtaaa agacatcagt
    10741 atggaaaacc tgaaaaaact atttacaggc gagatcaaaa actggagtga agtcggtgga
    10801 aaagatcagc cagtagttat attgaaccgc gcttcaggaa gcggaacccg tagcacgttt
    10861 gaaaaatggg tattggacgg agaaacagct gttcaggcac aagaacaaga ttcaagcgga
    10921 atggtgcgcc agatcgttgc cgacacaccc ggtgcaatca gctatgttgc tttttcttat
    10981 gtttccgatg atgtagatac gttgaatgtt aacggtgtag aaccgacaga tgaaaatgtg
    11041 acaacgaaca aatggacgat ttgggcatat gagcatatgt acacaagaga tcatccaaaa
    11101 aaactgacaa aagaattttt agactatatc ttatctgatg aagtacaaga taacatcgtt
    11161 ggagaactag gttatatccc tgtttctcat atgaaagtcg aacgtgattg gcaaggaaat
    11221 atcgttaaag agtaatcttt acacaaaatt tacaaactta gctaaacttt tacatgttat
    11281 taaaaccaaa ttgatgttca atttgtacac tgagtgagta tgttaaacct gattaagggg
    11341 gagtcatctg tggaagatgt gaaaaaagta ttattaacaa aatcaaaacg ttcaaaaatg
    11401 gaacagcgtg gaaaattcat cagtttttta tgtatcgcgc tgatcgtgtt ggttgtatta
    11461 tctatttttt attttgtagc tagtaaagga ttggccacct tttttactga taagatcaat
    11521 gtatttgatt ttctgtttgg tacagattgg aatccaagcc aaatcggagc aaatgggaaa
    11581 ccaatggtag gggcattgcc tatgatcaca ggttcgttta ttgtgacctt tttatctgct
    11641 atcgttgcca cgccatttgc cattggtgca gcagtattca tgacggagat ctctcctaaa
    11701 aacggaacga agatcttaca gccggtcatc gagctgcttg taggtattcc ttctgtcgtt
    11761 tatggtttta tcggtttatc agttatcgtg ccttttgtca gaagtatttt tggcgggact
    11821 gggtttggta tcttagctgg tacatttgtg ttgttcgtta tgatcttgcc gactgtcaca
    11881 acgatgactg ttgatgcatt aaaggcagtg ccacggcact atcgggaagc ttcattagcg
    11941 cttggtgcca caagatggca aacaatctac aaagtcgtgc tacgagcagc cgtgcccggg
    12001 attttaacag cagtcgtttt tggtatggct cgtgcgtttg gtgaagcctt agctatccaa
    12061 atggtcatcg gtaatgcagc attgatgcca acaggtctta ctactcctgc ttctacattg
    12121 acgtccatct taacaatggg aatcggaaat acaatcatgg gaacagttga aaataatgtg
    12181 ttatggtcat tagcgctgat cttgctattg atgtcattat tctttaatat cgtgatccgt
    12241 atgattggga agaaaggaga gctgaaataa tgaacgcaaa acgctcagat aaaattgcta
    12301 caggtgtttt atatatagtt tcggggatca tcgtattgat tttagcagct ttactacttt
    12361 atatcttagt tcggggaata ccgcacattt catggtcctt cttgaccgaa ccttccaaag
    12421 cctaccaaga aggcggtgga atcgggatcc agctatttaa ctcgatttat ttgcttttga
    12481 tcacgatgat catcagtttt cctatttctt taggtgcggg tatctatctt tcagaatatg
    12541 ctggaaaaaa ctggctaaca gatgtgattc gcacgtcgat tgaaatcttg agctcgttgc
    12601 cttctgtcgt tgtcggttta ttcggtttct tggttttcgt tattcaattc cagtatggtt
    12661 tctcgattat ttctggtgca ttggctttga ctgtattcaa cttgcctctt ttgactagaa
    12721 cagttgagga gtctttacaa gcggtccatt acacacaacg agaagcagga ttggccttag
    12781 gtttgtcacg ctgggaaaca gtgataaaag tggtggttcc agaagcgaca ccagggattt
    12841 tgactggtgt gatcctaagt tctggaagaa tttttggtga agcagctgca ttgatctata
    12901 cggctggaca aagtgcacct gcgttagatt ttacgaactg gaatccatta agtgtttcaa
    12961 gcccgatcag cattttccgt caagctgaaa cattagctgt ccatatttgg aagatcaata
    13021 cagaaggcac gatgcctgat ggtgtttctg tttcggctgg cgcttctgcc gtgttgatta
    13081 ttgctgtttt actgttcaac ttcggcgctc gtgcgattgg caaacgatta taccgaaaaa
    13141 tgacttcagc ctaaggagaa gaagcaaaat gaaaaaatat aactggaatg aaacaaatgt
    13201 catcaccatg ccaaaagaga aagaagtagc actatatacg gatgatctac atgtctggta
    13261 tggcgacaat gaagcaatca aaggagtgga tctggaattt gaaaaaaata agatcactgc
    13321 actgattggt ccttccggct gcggaaaatc tacgtatctt cgttcgttga atcgaatgaa
    13381 tgatgggatc gctaatacga aaatcactgg gaaaatcatg tatcaaggcg tagatattaa
    13441 tgcgcctcaa gtggatgttt atgagatgcg caaacggatt ggcatggttt ttcaacgtcc
    13501 taatccgttc agcaagtcga tttatgacaa tattacattt gctttgaaac agcacggcga
    13561 aaaaaacaaa aagaaattag atgaaatcgt tgaaacaagc ttgaaacaag ctgccttgtg
    13621 ggaccaagta aaagacagct tggacaaaag tgccttggct ttatctggcg ggcaacagca
    13681 acgtttatgt attgctcgtg cgatcgcgat gaaacctgat atcttgctgc tagacgagcc
    13741 ggcaagtgct ttggacccta tttcgacagg aacggttgaa gaaacacttg taaacttgaa
    13801 agaagactat acgatcatca tcgtgacgca taacatgcaa caagcagctc gtatcagcga
    13861 ctatactgcc tttttctatt taggaaaagt aatggaatat gacaaaacta acaagatatt
    13921 taccagacca aaaattcagg caaccgaaga ttatgtgtcc ggtcattttg gttaagacgg
    13981 aggcctcaac atgacaaaag agattatttc atctaaagat cttcatctgt attatggaaa
    14041 gaaagaagca ctaaaaggga tcgatctaag cttcaatcaa ggggaaatca ctgcgatgat
    14101 cggtccttct ggttgcggaa aatccactta tctgcgttct ttgaaccgga tgaatgacct
    14161 gattccgaat gtaacgatca ccggaagtgt tttatataaa gacaaagaca tctatggtcc
    14221 aaaaacagat gttgttgaac taagaaaaga aatcggcatg gtattccagc agccaaaccc
    14281 atttcctttt tctatctatg aaaatgtcat ctatggattg aaattaaaag gggaaaaaga
    14341 taaaaaagtc cttgatcaag tagttgaaga aagcctgaag gcagcgtctg tttgggatga
    14401 tgtgaaagac aaattgcata aaagtgcttt atctttatcc ggtgggcagc agcagcgtgt
    14461 atgtatcgca cgtgttttgg cagttaatcc ggaaatcatc ttattggatg aaccaacaag
    14521 tgcgcttgat cctgtttcta ctgggaaaat cgaaagtatg ctgttagaat taagagagcg
    14581 gtatacgatg atcatcgtga cccataatat gtctcaagct tcacgtattt cagataagac
    14641 ggcattcttt ttagatggtc acttgatcga atacaatgat acgaagaaga tattcatgaa
    14701 tccagaaaaa caagagacag aagattatat ttcaggtcga tttggataag gaggaaacgg
    14761 aatgctacgt acacaatttg aagaagaatt gctgaatctc cataatcagt tctacgaaat
    14821 ggggatgatg gtgagcagcg ccgtgcacaa gtcagttcgt gcatacatca aacacgataa
    14881 aatcttagcc caagaagtta tcgaaaacga tataaatatc aataacatgg aaacacgctt
    14941 ggagaagaaa agttttgaaa tgatcgcatt gcaacagcca gttacaactg atttgcgtat
    15001 gatcattacg gtcatgaaag ctagctcgga tttagaacgt atggccgatc atgcggtgtc
    15061 tgttgctaaa tccacgattc gcgtaaaagg gcagacgaga ataccggaaa tcgaaaaaga
    15121 gatctccgat atgtccgatt atgtgaaaaa aatggtggac aacgtcctag ttgcttatgt
    15181 caaaacagac gaaaaagacg cacgtacgat cgccaatatg gatcaaagag tcaatgacta
    15241 tttcaatcga atctataatg caacaatcaa aaatatgcag gaaaatcctg agacagtcat
    15301 cagtggaaca gactacttga atgttgccag ttatctagaa cggatcggag actatgtgac
    15361 aaacatctgt gaatggatcg tctacttggc tacagggaaa atcactgagt taaatacgaa
    15421 ccacaatgag attttctaaa ctgaagctgt gattatgccg tttgtttagg aaaagaggtt
    15481 gtgaaatcag cggttgtgaa aaagctgttc tgcatcaagt aataagaaca gtcaaacaaa
    15541 aaatagctcc tcatattttt cgtttggctg gacttattac cgcagatgca agcttttgaa
    15601 caccgtttat tcgggaaagg gtttgtgaca aaacatgtca caaacccttt tcatcatttc
    15661 atagaaagga taaagaatgc aaaaaaaaat tagcatcatc actatatttt ttatttgtgt
    15721 gcttacttca tgggttattc agcatcaagc cgctttatat gaagtacctg tcatcgagat
    15781 agaaaaagta cgaataaaag aggatcatca attgattaca ggaaaactga tgaacacgga
    15841 tcaaaaagga gaatcgattt atttatcggt tccttatcaa aaaaatcaaa tagaaacagc
    15901 aaatataaaa gcgagacaaa tgtatctagt ccaaatgcat ggagatgaat ggcagctagt
    15961 agcaaaaaaa aatgatggct ggcttttctt cttcacaagt atcttcattg gcgccatgtt
    16021 gcttattggt ggaaaacagg gtctatttgc tttaggaagt ctcatcatca atatcgtttt
    16081 acttttactt ttgttgctca tctttactaa aacagaaggg atttctctat taaatatatc
    16141 tatcctgttc gtcgttatag ggatgattat tagcatattg gctttagatg gatggaatag
    16201 aagcagtata atcaaaattt cagctgcagt tacgagtgtt tttttagcat tttttatttg
    16261 tcttttgaca atgaatcatt ggaaagacca aggactgcgc tatgaggaac tgaattatct
    16321 gacacgaccg tatcgttctg tttttctttc tagcgtactg attggaacag ctggaggaac
    16381 actcgatacc gttatcacag tcattgcgac attagaagaa ttgactaagc gacaaccaga
    16441 aataacagcg aaacagttat ggctttcagg aaggcaagtg ggtcgagatg tgattggctc
    16501 gatgaccaat atccttttgt tcgtctatat cagtgggtca gttccatcgc ttcttttata
    16561 tttaggaaat caatggtcgt tcaaagaaac gatagaacag catttatcat tggaattttt
    16621 acgcgtcatt gctggcagct taggaatagt attatcgatc cctatctcgg tcctcttttt
    16681 tctgactgta aggaggctga aacgatgaat gtacttatta tacttgttgg gattttagca
    16741 atcagcgtgc tacttattgg cggtacgcaa ggtatgtata ttcttttagg cttattcatc
    16801 aatttaggga tctttttcct gttgttattc ggttatcatc agaagtggcc gatactggca
    16861 ttgtcggtca taggattttt attgattgct gtggttatct tattttttat caacggttat
    16921 aatctaaaaa tgcgtgcagc ttttgtgagt atactgatct ttttattttg tttcctacta
    16981 ttgcttccta tcactgattt tttagcaatc caaggtttta catcggtaga actagaagaa
    17041 ttatctggct tagataagac tttagcgatt gattttagat tacttacgag atcgttgctt
    17101 ctgattagtc ttagtggtgc tgttttagat gcgagtgtag caatttctag tgggacattc
    17161 gaagtatatc aggcaaatcc gcaattatct ttttaccaat tgcgccattc tagtttcgct
    17221 attgctaaaa aaatattggc gtcaacagta atgacacttc tttttgcatt catgggaagt
    17281 tctttagcgt tgatcctctg gtttattgat ttagacattc cttttcagca agtgatcaat
    17341 gaaaaatcat ttgttctaga atatacgatg gctattctta ccacattgtc cgcactactt
    17401 gttcttcctt taacctgcgt gactacagca tatctcttta caaaaaagaa agaacaactc
    17461 aagatggagt aaaagttaca acttaagtcc tatgacattt ttactgaatc agtgcataat
    17521 atggatgtaa ggaaaagctt gcaaacaatt ttgcttaagg aggcaatttc catgaaagaa
    17581 agagaacgta ttttagattt agtgaaaaaa ggaatccttt cgactgaaga aggcttagat
    17641 ttattagaaa gcatggcaac tgaaaaagac gaaaagcaaa tcaaaaaaga agcagacaag
    17701 gtaactgcta gtcataaaga aaaagatcag gcaagtcaat tgatcgataa acttgaaaac
    17761 ggcgaagaag aaatttctga gccagttgat ccaaaggaaa gagaaagaca agatcaagaa
    17821 aacttagaaa agatcttaga tgaactagca actgaagcga ataaaacttc ggcgcgtcta
    17881 gatgaagtaa atgctcaatt cgctgacaat aaggcagcac gcaatgagaa acaggaagca
    17941 ttgatgcagc tgaatacaaa agaagaatta ggcgagttga cagaagaaga attagcacag
    18001 cgtcagacgt tagaagcaga aatcaaagaa ttagaagcta gtggcgatac actgttagaa
    18061 gaacaattga aattagaagc agaattaaaa gatatccgta agaatcaatg gtctgagaaa
    18121 aaagaaacgt ttactgataa gtttgaattg ccagatgatt ggaaagatca agcaacagat
    18181 acactgaatc aagtaggcga aaaaatgtca gaagctggat cacagttagg caaattcttg
    18241 aagaaaacat tccaaactgt ttcagaaaca gtgaatgaca acatggaatg gaaagatgtc
    18301 agcttacgtg tccctgggat tgcgacaacg aaatttgagc atgaatttta ctatgaagct
    18361 cctgcagcaa gtatccttga tatcaaggca gcaaacggaa acgtgacgtt aaaaacgtgg
    18421 gacagcaacg atgtgaaagt tgaagctaag atcaaacttt acggaaaaat gggtgctgaa
    18481 ccatttgaag cattctctga acggagccag attgaggtta atgaagatca tatctccttc
    18541 caaattccaa ataaacgtgt gcgtgcagac cttgttttct acttacctaa acgagtatac
    18601 gaccacgcag ctattaaact tctaaatggc aacatcatga ttgaaacatt ggaagctaaa
    18661 gatatttata caaaatctac gaatggtaac atcatcgtgg accaattaac tgcaacgatg
    18721 ctggaagtag aaggtgtaaa cggcaatatc gatattcgaa acgggaacat cttagattct
    18781 attatcgaaa cagtaaatgg tacagtgaca tttggtgcga cagcagaaaa cctttctgtg
    18841 tctttggtaa atggtgatgt acgtcttacg attaaagaag ataatttgaa aaaagtagaa
    18901 gcaagttcag tgaacggaaa tgtcaaagtc gctttaccag atggcatcgg tttggaagga
    18961 catgctaaaa caagtcttgg aagtatcaac agccgtctgt ccaattacga agtagttcgt
    19021 gagaaaaaag aacggacaaa ccaaatgttg caattccgtc gtgtttcaga tggtgaaatc
    19081 gcgcaagtgc aactttcaac gacaacagga agtatctatt taaaagatac agataaataa
    19141 agaggttgtg aaagagatgt tcagccttga gcaataagaa gcgattttgt aaaatagctt
    19201 cccctatttt acaaaattgt gccttattgc cgatagtcta tctcttgaac accgtttacc
    19261 aagagattat gaacctgcca attatcgccg agtcacaagg aataatttca gaaaaagctc
    19321 gtcacgtttt atgaatatgg taatttgtga ccgagaattt tgcagaaaga caccgattaa
    19381 gaagaaggtt gtcacaacga ttttcttcat ttaaaatagt agaacagagg tgagacatga
    19441 tgaaaaaaaa gttgacaaaa tctaataaaa atgttgtttt gacagggact ttagcaggaa
    19501 taggagaata catcgggatt gatccgactg ttatccgtgt gctttatgta ttcctgagct
    19561 tagttgcttt tggaagtcca attattttat acattttatt agctttgatt atcccatctg
    19621 cgcgttctgc acgaacaaac tatggacata acaatgcata ttatgaaaaa aacaattatc
    19681 gtgaacaaac tcgtgaacaa acaaaaaaac gcaaagaagc ggaaaaaatc gatgatgacg
    19741 aatggagtga cttttaatgt cgtactttca acgtatttta gttaatacgt taacattcat
    19801 atcattggct gttctttttc ctgcgatggt ccatgtgagc agtatttgga cggccgttct
    19861 tgccagcttc gtattgtctg tgttgaatat gtttgtcaaa ccgattttga cgatactgtc
    19921 cctgcccttt acattagtca cattgggatt gtttacgttt gttatcaatg cagcgattct
    19981 taaaatgacc tccttttttg tgggagcaga acattttagt ttttcttcat tcggtgcagc
    20041 gttgttagta gctatcttga tgtcgcttgt caatgtaatt gtcagcgaac ataacagtag
    20101 aaaataaaag aaagctgtgc cgaactatcg gcgcagtttt tttatttaga atcaggaatg
    20161 cgttttctat tacattggta attaaataaa aacgtttttt ttaattggta gtacttttgg
    20221 ctagacaatg ataaaatgat agagaacact ctaatagaaa tgaggtagca tgaatgtcag
    20281 aagttgtaaa agttagccaa ttagtgaaaa aattatcttt ggaaatcgtg acaggcgacg
    20341 agaaaagctt agaccgtgtc atcgtaactg gggacatctc acgcccagga ctagagttga
    20401 ctggttattt taattattat tcccataatc gtttacagct atttggcagt aaagaaatca
    20461 gttttgcgga acggatgatc ccagaagagc gtttgatcat catgagaaga ttatgtagtg
    20521 aagatatgcc cgcatttatt atttcaagag gattaccagc tccagaagaa atgatccagg
    20581 cagcaaatga aaatgggata gctgtactgc gttctcccat ctcaacttct cgtttattag
    20641 gagaattatc cagttattta gatggtcgcc ttgcccctcg aacaagtgtc cacggtgttt
    20701 tagtggatgt ttacggatta ggtgtactga ttcaaggtga cagtgggata ggaaaaagtg
    20761 aaacagcgtt ggagttgatc aaacgcggcc atcgattgat cgcagacgat cgagtagatg
    20821 tttatcagca agatgaatta acggtgatcg gagaaccgcc taaaatcctg gaacatctga
    20881 ttgaaatccg aggaatcggg atcattgatg taatgaatct ttttggtgcg agtgcggtac
    20941 gtggctctat gcaagtccag ttggctgttt atctggaagc atgggcaaaa gataagaaat
    21001 acgaccgtct gggctctgaa gacacgaccg ttgaaatcgc ggaagtgggc attccacaaa
    21061 taaaaatccc tgtcaaaaca ggacgaaacg ttgcaatcat cattgaagta gccgcgatga
    21121 atttccgtgc acgtacaatg ggctttgacg caacgaaaac atttgaagaa agattaagtc
    21181 gcttgattga agaaaactca ggcaacgact agcaaggagt gacaatatgt tagcacaaat
    21241 caatcgagtt gcttttgagc ttgtagggat tcctatctac tggtatgcat tgatcatcgt
    21301 atcaggtatc attatcgcta tgtggcttag cagcagagag gctgttcggg taggattaaa
    21361 agcagatgat gtgacagatt ttatgttgtg gggattgcct atctccatta ttggcgctcg
    21421 cttatattat gttttatttg atcttcccca gtatatagct gatccaatcc aaatattcaa
    21481 tatccgttca ggaggcttag ctatttatgg cggactgatt gcaggcgcga tcacgctgta
    21541 tttttttact aaatatcatt ttatttcttt gtggactttt ttagatatcg ctgcaccaag
    21601 tgtattatta gcacaggcaa tcggtcgttg gggaaatttc atgaaccacg aagcatatgg
    21661 accagacacg acgcgaagct tcttagaaag tctgcattta ccgcaattca tcattgataa
    21721 tatgtatatc gaagggatct atcgtcaacc aacatttctt tatgaatcac tttggagttt
    21781 gattggattt attttgcttt tgtttttacg aaagaaacca caccttttga aaaaaggcga
    21841 agtcctttta tgttatctaa tgtggtatag tttcgggcgt ttctttattg aaggattacg
    21901 tacagacagt ctttactttt taggtgtgat ccgtatttct caattattat ctgctgtgtt
    21961 atttgtcggt acattgattc tatttatttg gcgcagaaaa aaaggaaatt taccggatta
    22021 tgaccgctca ttaggaaaaa atcaacaaat tatctgattt ttagagcgtt aaaaatgaaa
    22081 aaaatttcag gagtggtaaa atgaattata gagtagcggt tttaggacca ggctcttggg
    22141 gtactgcttt ggcacaagtc ctcgccgaaa acggacatga cgttcggatt tggggacatc
    22201 atgcttcaca agtagaagaa atcaacaccc aacacacgaa tcaacgttat ttaccagatc
    22261 attatctacc cgattccatc caagcattca ccaatatgga agatgcagta aaagacgcag
    22321 atgcggtgct attcgtcatt ccaacgaaag caatacgttc tgttgcaaaa gaactagtgc
    22381 ctaaattgaa tacaaagcca gtgatcatcc atgcaagtaa aggattagaa cagggaacac
    22441 ataaaaggat ctctgacatc ttgttagagg aaatcccagc agaaaaaaga aaagctgttg
    22501 ttgttctttc tggaccaagc catgcagaag aagtagcaaa acatgacatc actacgatca
    22561 cagctgcaag tacagatgca aaagcagcaa aatatgtaca agagctgttc atgaataatt
    22621 attttcggat ctataccaat cctgatgtga tcggtgtaga gatgggggca gctttgaaaa
    22681 atattattgc tattggtgct ggcgctattc acggcttagg gtttggcgat aatgcaaagg
    22741 cagcaatcat gacgcgcgga ttagctgaga tcagccgttt aggtgtagca atgggtgcca
    22801 acccgctgac ctttatcgga ttaagcggaa taggcgatct agtcgttact tgcacaagtg
    22861 ttcattctag gaactggcgc gcaggcaact tgttaggaca agggcagccc ctagaagagg
    22921 tgctagccaa tatgggaatg gtcgttgaag gtgtgaatac tacccaagca gcgatggaac
    22981 tagcccaaat gatggaaatc gaaatgccca tcactcaaac aatctatgaa gtgctgtatg
    23041 aacaaaaaga aatcaaaaca gccgcaaaag aaatcatgtt acgtgatgga aaaaaagaaa
    23101 atgaattctc attaacaatt ttttgaagga gcgaatgtat atgaaagtta aaaaagcagt
    23161 tattccagca gcaggattag gaacaagatt tttaccagca acaaaagcta tggcaaagga
    23221 aatgttgcca attgttgata aaccaacaat ccagttcatc gttgaagaag cgttgaaatc
    23281 aggtatcgaa gacatcttga tcgttacagg aaaagctaaa cgaccaatcg aagatcactt
    23341 tgattcaaat attgaattag aaacaaacct aagtgaaaaa ggaaagaccg aattattgaa
    23401 attagtagaa gaaacgacag atgtcaactt gcatttcatc cgtcaatctc atccaaaggg
    23461 tttaggacat gccgttcttc aagcaagagc ctttattgga aatgaaccat ttgtcgttat
    23521 gctaggcgat gatataatgg aagacgaagt accattgaca cagcaattga tcgatgacta
    23581 tgagcgtaca cacgcttcta cgattgccgt tatgcaagtt cctcacgatg aaacttcaaa
    23641 atacggaatc atcaacccag gggaagtact ggaagatggg ttatacaatg tcaaaaactt
    23701 tgttgaaaaa ccagatccaa gcaaagcacc aagtgattta gctattattg ggcgttactt
    23761 attgactcca gaaatctttg atgtattatc agaacaacaa ccaggtgctg gaaatgagat
    23821 ccaactaact gacgcaatcg atacgttgaa taaaacacaa cgcgtatttg cccgtaaatt
    23881 cacaggtaaa cgttatgacg ttggtgacaa atttggtttt atgaaaacaa gtattgaata
    23941 tggtctgaca catccagaag taggggcacc tttgcgtgaa tacatcatgt ctttaggtaa
    24001 aacattagaa gcagaaggaa gcaagatcaa tcataatcca aaaggaataa cagaaatcaa
    24061 agaagaaaag aaaaaataat ctttttttct aacgagcttc agcggctaat tgaataaaaa
    24121 gtagttgaag ctcgttttta tttgtttaaa aaacacgaaa atgctatcct tatagaaaag
    24181 agagacaaga agggagtttg atcatgtcag gaggagaaat tgcaggattg atcgcagcaa
    24241 tcgcctttgc gatacttgtc gttttcatcg ttcgcgtatt gttgcaagtt tccaaaactt
    24301 tagaaaaagt agaccagact gtcgcagaag caaatacgac aattgagatc gtcacacaag
    24361 acgtagatct tttatctaga caagtagaag gcttgctagt caaaagtaat gagctgctct
    24421 tggatatcaa tggaaaagtc gcaacgatcg atccattgtt cacagcagtt gctgatttaa
    24481 gtgaaagtgt atcggaactt aatcattcta gtaaaaacat cgtgacgaaa gtcggcggaa
    24541 tcggaaaaac gacagctaaa gcgactgttg ccggaaaagt cggtggaaca gcgatgaaat
    24601 tttttaaaaa caaaaaacat actgaaacga caggaggaaa ataagaatgg ctaaaaaagg
    24661 tggattttta ttaggtgcag tagttggagg tacagccgca gcaatcgccg cattattgtt
    24721 agcacccaaa tctggtaaag aacttcggga tgaattggct gctcaagcag atgatttgat
    24781 ggacatggct tcagattaca cagatatggc aaaagaaaaa tcatctgagt taacaggtat
    24841 tgcaaaagaa aaagcgcaag gtctttctca acaagcaggg gatctagcag gcaatgttaa
    24901 gcagaaggta agtgaatcag cagaggaagc aggaagtgaa tcagatgata tcttgttaaa
    24961 cctcaaagaa caatcagctg atttatctaa tcgtttcaaa aaaacagcag aagatctgaa
    25021 aaagccagca gacgaattag gtagtattgc taaagatact tcagaagata tcatgatcga
    25081 tgtaaaagat gctgcagata aagcaggaga gacggtcaat gaaggaatgt cagaagcaaa
    25141 accagttgca gatgacacaa aagaaatgtt gaaaatggca aaagaagatg caaaagaagc
    25201 taaaaaagaa atgaaagata caatgacaga ttcgactacc aatcactaag attactcatt
    25261 tctagaataa aaaaggtgag agcggaccag ctctcacctt ttttattttc attactctaa
    25321 aacaagtaat tcttttggtg tagtcgtgaa tggcacacaa ccatcacttg tcacatacac
    25381 acaatcttcg atacgtaccc ctactttttc aggaatatag attcctggtt caagagagaa
    25441 gcacatccct tcttcaatca ctaagtcgtt tccttctaca agagaaggga attcgtggac
    25501 ggttgttccg attccgtgac ctaaacggtg attgaaatat tcgccgtagc catagctgtt
    25561 gatcacgttt cgtgcgattt gatcaagttc acctgctgtc acacctgggc gaactgcctc
    25621 ttgtgcggca agctgtgctt caagagtgat gttatagatt ttttcttgga actcagttgg
    25681 tttttgataa gcaactgtac gagtagcatc actgcaatag ccattccaaa taacgccaag
    25741 atcgaataac actagttcat ttggtgaaac cttcgtgctt cctggtgtac catgagggct
    25801 tgcagcattt gctcctgcaa ggactaatgt gtcgaaagac atttgtgaaa caccttgttt
    25861 ttttaattgg tattcgattt ctgcgataat tgcttgttca gttacgcctt cttttactgt
    25921 tttgaagcca atttcaaagg caacatcggc ccatttacca gcttcgatca gtttgtcttt
    25981 ttcttctacc gttttgatca gttgcagacg ctggataata gaagttagat cggtgtaaga
    26041 atctgtacct gtcaagatat cagagatagc atcgaagcga tcgacagtca gagcattctt
    26101 ttcaatacct acattaccag gttgcccata gcgtgcgcga agctcttttc cgatgatctc
    26161 ccaaggattt tcgctgtcta agtagccacg tacatcgtac gcccagctgc tgttttgagc
    26221 atcttcaact tccagagcag gagtgaaaag gaaaggatct tgttccagag ggataaaaag
    26281 agctagtaca cgctcgtgag gatcactttc atatccggaa aagtaagcga tgtggccagg
    26341 gtcgctgata taagccaatt ctacttggtg ttgtgccatc cattgacgta gttcattgat
    26401 ttttacgtta ttcatttggc aacctacttt ctaaattaat tttgtattca ataattgtac
    26461 cactgattga ttaaaagacc aacgttctta aaactgaaaa aagtaaaaat tttcatgaaa
    26521 acagagataa aacggttgca attgttgaga ttatttgata tcctatttta gaaatgtaag
    26581 acgtattttc agaaaacata taaaatatga ataggagaac acaaatggaa aagcaaacaa
    26641 tcactatcta tgatgtagcg agagaggcaa atgtttcgat ggctactgtt tcacgtgtcg
    26701 taaatggcaa tccaaacgtc aaaccagcaa caagaaaaaa agtcttggaa gttatcgatc
    26761 gcttggatta tcgtccaaat gcagtcgcac gtggcttggc aagtaagaaa acaacaacag
    26821 ttggggtaat cattcctgat gtcagcaata tgttctttgc ctctttagca cgtggtatcg
    26881 acgatgtggc aacaatgtac aaatacaata tcatcttagc aaactcagat ggcaacgatc
    26941 aaaaagaagt caatgtattg aataatttat tggctaagca agtggacggc gtgatcttta
    27001 tgggtcacca tatcacagac gaaatccgtg gagaattttc acgttcaaaa acgcctgttg
    27061 tactagctgg ttccattgat ccagatgaac aagtaggtag cgtaaacatt gattatgtgt
    27121 cagcaacaaa agatgcagta aataaattag caaaaagcgg caataaaaaa atcgcttttg
    27181 tcagcggtgc gctgattgat cctatcaatg gtcaaaaccg tctaaaaggc tataaagaag
    27241 cattaagcga aaacaatcta ccatatagcg aaggattgat ttttgaggca caatacaact
    27301 ttaaagatgg tctgtctcta gttgaccgga tccataatag cggtgcaaca gctgtctatg
    27361 tttcagacga tgaattagcc attggtatct tggatggttt attagaccgc ggcgtaaaag
    27421 taccagaaga attcgaaatc atcacaagca acaattcttt actgacagaa gtagctcgtc
    27481 ctcgtttgac aagtatcaca caaccattat acgatttagg agcggtatcc atgcgcctat
    27541 tgacgaaaat gatgaataaa gaagaagtcg aagaaaaaac gattatcttg ccttatggaa
    27601 tcgaagaaaa aggatcaact aaataataaa agatcaatca tttaaaggtt gttccaaaag
    27661 tggtcaactt ctggaacaac gtttatacga gaaagggggt tatgacaaaa ttttgtcata
    27721 accccttttt ttttgtgact ttaaaaaaag agaaaatggc ggaaacaaac cttacgtcca
    27781 tcgtatacga cacaatctgt gcgtagatgc tacattttgt tcactactat caaaattact
    27841 ttaaaatgaa aacgtgataa aaaagaaagt agaaaagtag ataagtaact ctaaaaaatc
    27901 caaaaaaatc caggctcgat tgcgtactta tcttaaagga gaaacaatcc atcatatata
    27961 acccgaaagg agacttctat ttgcctaaga aaagacgaaa gaccatagtc atcattttga
    28021 ccattcttac tataggaatc ggagggaaat tttacatgga caaaagagaa atgcaaagac
    28081 agaaagattt actcgcagta gaaaaacagt ccgtcaaagt tctgaaaaat acctttgcgg
    28141 atatcaagga agtgaagatt gaaaaatctg ctcgaaatga aatgacgggt tcataccgga
    28201 tagttattct aatgactaat aaacaagatc aatcgattta tttttcttat agtttttgga
    28261 aagaaagaaa tgagatagga agttacggaa tagttgatga aaaaaagcaa aaagaaggaa
    28321 atacgctcaa taaagttaaa gtaacttact ctaatggaaa tgaggaaagt atttaatgat
    28381 tactgacaaa caatatagtg aattaagtga tgctgtttac tggctagatc ctaaacatag
    28441 agactatgta cctgaaatgc aagaaaattt aagttttaaa attaacggca ctatgtataa
    28501 aatcctaaaa atcaaaaact ccttcgacgg catgcaggcg atggctgtag cgccaatcgt
    28561 ccatagcaaa ttggagaaaa atttcaaaaa taaaaaaatt cctgcaaact ttcgcgtact
    28621 taaataatga aggtgtcaat acttacataa aaagaaagga ggcacgttct tgtcaaggaa
    28681 aataagaaaa ctcagcttag ccattttatt aatcgtcata ttagggatcg gagggaaagt
    28741 ctatatggac aaaagagaag cacaaaggca gaaagattta ctcgcagtag aaaaacagtc
    28801 agtcaaagtt ctgaaaaata cctttgcgga tatcagggaa gtgaaggtag aagaatttaa
    28861 aaaaaaccct gttaccggaa gttatggtgc acttgtgaca atgacaaata atgaagggaa
    28921 atctgtttat tttgattata gtttttggaa ggagagaaat gaacttggtg gttttggcat
    28981 tgagaacaga gcggtacaaa agacaggagt cactataaaa agagtaaaag taattttttc
    29041 taacggacag gaggagttag tatgacaata aacgataagc attacaatga tattagtgag
    29101 aaggtttatt ggctagatcc caaatatccc cgctacaatg agggatacaa aaagaactct
    29161 gttaaagaat ttgctggtat ggaatttcaa atcctacaaa tcaaagactc attggacggc
    29221 atgcaggcga tgtctgtagc gccaatcaag aacggtaaag tggatctttc tcaagtagtg
    29281 attgcttttg caggaacgaa cttaagtgat tggaaagatt tggaaacgga tgcaagaagt
    29341 atcatgaatc tctacgggaa tagaccagga gcaagttttt ctgaaaaagc aacaacaaac
    29401 aagattttat ccgttagtgg attgaaaaca gcggggcaaa tcaattcagc gattgcattt
    29461 gctaatgaag taaaaaagga atatggagaa gctgctattt ctactaccgg ccattcatta
    29521 ggtggattct tagcattata tgtagcagca gagaaacaat ggaaaaatgt cggcttcaac
    29581 gggccggacc cgtatgatct cttatcacca gaagcaaaaa aatgggtgga agaaaatccc
    29641 ggtatgctca ccaattaccg aaatcacgcc gattttatcg gtaattttgg tggtaatggc
    29701 acaggtgctg aagttcggat cagtatggaa acagcattct tgaatctgct tgcttcccac
    29761 agtttaacct cttggaagtt tgatgaaaat ggaaagctga ttattcctga aaatgaaaac
    29821 aatgttaaag ctcgaaaaca acagaaagaa aaccaagcga cccagttgtt ttcctatcag
    29881 ttgcaagaac tgaacaacat acgtaaaaaa ctacgattaa gtggcggcca gctgacagtt
    29941 aatgaacaaa tttacttaga tgacacacaa gctaggttag tagtggaaaa gttatcggca
    30001 aaaatgaaaa gcgctatgga agaagttatc cgcatcaatc aaagaggaat ttttgagcta
    30061 gagaaaaaat ggcaagaagg cttgcggcaa gcacgtgttg tagccccttc gttgactgaa
    30121 caggaaatca tagaagcatt agctagtgtg ggcgccacaa aagaaaactt agtcgattct
    30181 gcgaaagaag tctatctgct acgaatcaat aaggcaagac gtatggggga ggcctttgat
    30241 acattggcga aggagatcca agacaaaatc aatgaaatcg tggcacgaga tcgagaatta
    30301 gcacagcaat tcaattaaag gagaagtaga tgaaactaga agaattggaa agagattatc
    30361 gaagtgaacg aagacaatta gaagctcaag aagatgaatt gatccacttt aagaatcaag
    30421 gattacgatt gatggagcaa gaggagcagc gactgcatca tgtattaaag aacttagctt
    30481 tagataatga gcctctgttt caagcaagac gaagttattc gaattatcag gagcattatc
    30541 aacaagccct acaagtagaa cgacgaaaac tctatcagca gcaagatgaa ctagaacgtt
    30601 cctatcgtca aaaaatcaga caaataacta tggagaaatc atcttatgaa tgaattttta
    30661 atgcaattac taaaagaatt agagaaaaat ctacatcgat caaatgtacg ttcagttggt
    30721 atggggatat ctcagcaaat ttgggcagaa aaacgattga cagatcaaaa ctaccaaatg
    30781 actgcagaag aagctgctca agaattttct aaccgacatg tacacttgaa aggatcagta
    30841 aaagggcaat ttgcagaaaa actaactgaa ttattccaaa gacaagaaaa gaaattaaaa
    30901 cagtttcgtt agtaaagtta tatagaaaaa ttttgtgaaa atggttt
//
LOCUS       DACEUZ010000016        30766 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.016, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000016 DACEUZ010000000
VERSION     DACEUZ010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30766
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.016"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(338..1291)
                     /locus_tag="IXG44_000663"
     CDS             complement(338..1291)
                     /locus_tag="IXG44_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ1657402.1"
                     /translation="MKKLVVGILILASFGLAACGNSDNTSQADTKKSSTQAETALTIK
                     DSNGDQIEVPKNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(1314..2069)
                     /locus_tag="IXG44_000664"
     CDS             complement(1314..2069)
                     /locus_tag="IXG44_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1657403.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKKLVQEIGKTIVIVLHDINFAASYADEIIAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            complement(2066..3025)
                     /locus_tag="IXG44_000665"
     CDS             complement(2066..3025)
                     /locus_tag="IXG44_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAQ1657404.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFAWALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            complement(3018..3968)
                     /locus_tag="IXG44_000666"
     CDS             complement(3018..3968)
                     /locus_tag="IXG44_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAQ1657405.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFPQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            4118..4570
                     /locus_tag="IXG44_000667"
     CDS             4118..4570
                     /locus_tag="IXG44_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HAQ1657406.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPVEKEKPKKKKSFWDRLF"
     gene            complement(4658..6925)
                     /gene="gshAB"
                     /locus_tag="IXG44_000668"
     CDS             complement(4658..6925)
                     /gene="gshAB"
                     /locus_tag="IXG44_000668"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HAQ1657407.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEAIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDGIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(7112..8110)
                     /gene="ftsY"
                     /locus_tag="IXG44_000669"
     CDS             complement(7112..8110)
                     /gene="ftsY"
                     /locus_tag="IXG44_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HAQ1657408.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKIEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            complement(8122..8931)
                     /locus_tag="IXG44_000670"
     CDS             complement(8122..8931)
                     /locus_tag="IXG44_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ1657409.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMVHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            complement(8940..12521)
                     /gene="smc"
                     /locus_tag="IXG44_000671"
     CDS             complement(8940..12521)
                     /gene="smc"
                     /locus_tag="IXG44_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HAQ1657410.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLLKEVAEKETEIQKAEASLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYVQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            complement(12541..13227)
                     /locus_tag="IXG44_000672"
     CDS             complement(12541..13227)
                     /locus_tag="IXG44_000672"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAQ1657411.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            complement(13575..15356)
                     /locus_tag="IXG44_000673"
     CDS             complement(13575..15356)
                     /locus_tag="IXG44_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ1657412.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            complement(15382..16296)
                     /locus_tag="IXG44_000674"
     CDS             complement(15382..16296)
                     /locus_tag="IXG44_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1657413.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            complement(16323..17285)
                     /locus_tag="IXG44_000675"
     CDS             complement(16323..17285)
                     /locus_tag="IXG44_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1657414.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            complement(17285..18229)
                     /locus_tag="IXG44_000676"
     CDS             complement(17285..18229)
                     /locus_tag="IXG44_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1657415.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            complement(18230..19234)
                     /locus_tag="IXG44_000677"
     CDS             complement(18230..19234)
                     /locus_tag="IXG44_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1657416.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            complement(19502..19744)
                     /gene="acpP"
                     /locus_tag="IXG44_000678"
     CDS             complement(19502..19744)
                     /gene="acpP"
                     /locus_tag="IXG44_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAQ1657417.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            complement(19866..20867)
                     /gene="plsX"
                     /locus_tag="IXG44_000679"
     CDS             complement(19866..20867)
                     /gene="plsX"
                     /locus_tag="IXG44_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HAQ1657418.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            complement(20916..22952)
                     /gene="recG"
                     /locus_tag="IXG44_000680"
     CDS             complement(20916..22952)
                     /gene="recG"
                     /locus_tag="IXG44_000680"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HAQ1657419.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKEFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LLTGSTKAKERRELLEKLEQGEINIVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            complement(23069..24751)
                     /locus_tag="IXG44_000681"
     CDS             complement(23069..24751)
                     /locus_tag="IXG44_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HAQ1657420.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKETPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            complement(24803..25165)
                     /locus_tag="IXG44_000682"
     CDS             complement(24803..25165)
                     /locus_tag="IXG44_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ1657421.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            25435..25623
                     /locus_tag="IXG44_000683"
     CDS             25435..25623
                     /locus_tag="IXG44_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HAQ1657422.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            25861..28530
                     /locus_tag="IXG44_000684"
     CDS             25861..28530
                     /locus_tag="IXG44_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAQ1657423.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEVSFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEKPRNTHRRRRRK"
     gene            complement(28621..29241)
                     /locus_tag="IXG44_000685"
     CDS             complement(28621..29241)
                     /locus_tag="IXG44_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ1657424.1"
                     /translation="MFLINATDEILSVNKVFYTVPAEKMSRVSSDINTITSRIGLTKA
                     MHMVLKYNQEDPPVPKTYEAIDLIIVDEIDRLKLQHLEQLRDIYDRNDLAMVLIGMPG
                     IEKRLSRYPQLYSRIGFAHEFDNLSKDETHHILEYKWADLGIDIKLEDFSDYEAITTI
                     IKITKGNFRLIHRLFAQIDRILRINQMDKITVEVVEAARDSLVIGI"
     gene            29353..29754
                     /locus_tag="IXG44_000686"
     CDS             29353..29754
                     /locus_tag="IXG44_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1657425.1"
                     /translation="MKKLFSALFILLFGFIGIGTSGNIVKASTMVEQTSEMAKTFSQL
                     TPEEQSYFKSKGFSDKDTFYTQKIEPQDPKTRFSANVLRLTASTKKITNTQGRTEYII
                     TSPNSAMYRISTRLSLGSAKSYTQTVNLANS"
     gene            29889..30227
                     /locus_tag="IXG44_000687"
     CDS             29889..30227
                     /locus_tag="IXG44_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic metallochaperone ArsD family protein"
                     /protein_id="HAQ1657426.1"
                     /translation="MIISYYVLNRSDDEGLFVFQSLHEDTMLKEFYNRIKNKQIETNL
                     YFLSDSILPFISNPKILNLLQTKDLKSLPIIVIDEEIVKYGAYLSVEELEKLTNIGMS
                     IQEDNGVHGT"
     gene            complement(30608..>30766)
                     /locus_tag="IXG44_000688"
     CDS             complement(30608..>30766)
                     /locus_tag="IXG44_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HAQ1657427.1"
                     /translation="IKNHEKFLIRNYKISGGGCYLLECRFTSKKSMNSFLEQLNEYAN
                     YKLSIVID"
BASE COUNT         8598 a         6498 c         5447 g        10223 t
ORIGIN      
        1 agaagagatc ggtacaaaat aaaaatatct aaatttaagt tttatctaag tttcgctttg
       61 tacctttgat tcaaatcaaa ggaggcgatc ttgatgctga aaaaatggat cacactattt
      121 tttgccactc cccattctag aatcaacccg tatgatgtag agaatgaatt cacgttgaaa
      181 aaagatttcc caaataaata aaaaccatcc gtaaaatcat caatacgatt ttacggatgg
      241 tttttgataa cttctttgag gataggctct tgctatttaa gaatgtaaga aacattattg
      301 ttacatagta tgagagttta atcctctgtt atttatgcta tttaaatgct tgattcacat
      361 cttcgatcat cagtttcatt gattctaaac cgcccccgct taaataccag acatctggtt
      421 caagggagat gatctgctga tttttaccag cattcgtttg tgctaccagt tcattggcag
      481 aaatatcatc tttgctgtcg tctccaccaa tagcttttgt tctgtctact acaaataaga
      541 tatccggatt tttttccagc acatattcat aagaaacact ttgaccgtga gtggatgctt
      601 cgatttgatc atctgcttgt tcaaagccaa ataaatcatg gatgaaaccg aatctagaac
      661 cagatccgta ggctgataat tgcccttcat tcacaagtgt gactagtgct tttttgtcag
      721 tagcttctgc tttctttttc gtctgatcga ttgcactaga taattcctct aattgagttt
      781 tagcttcttc ttgcttacca aagatctccc ctaaagcagt gacattttgc tggaaagatt
      841 cccatggatt tttcgcatct aaagaaagga aaatggtcgg agcaatttga cttagatctt
      901 tttgaaaatc tctttgccgg ccagaaatga tgatcaggtc tggctgaagc tgattgattt
      961 tttctaagtc tggttctttg atcccacctg ctgattccac ttttttatag gatgaaagat
     1021 actcaggaag gctgcttgta gctgctccaa ctaccttatc tccaacacca agagcatcca
     1081 tcgtatctaa agaaccattg tcgaatacga cgactttttc aggattcttt ggcacctcga
     1141 tttgatcgcc attactgtct tttatcgtta atgccgtttc agcttgcgta ctgcttttct
     1201 ttgtatctgc ctgactggtg ttgtcactat ttccacatgc tgctaaacca aatgatgcta
     1261 gaatcaagat acctactact aattttttca tctcttctct cctaatgtta gttttagaaa
     1321 taaagacaga atctgcgacc ttcaatctca caaattcgta tgtccatttc gtacaattcg
     1381 tccagtacat ctttttttat catttcttgg gtcgttcctt gcgtgaataa ctgcccatct
     1441 ttcattgcaa taatttcgtc tgcataactt gcagcaaagt tgatatcatg aagaacaata
     1501 acaattgttt tcccgatttc ttgtactaat ttttttaaga tctgcatgat ttgaaccgca
     1561 aaattcatat ctagattgtt caaaggttca tccagtaaaa tataatccgt gtcttgcgct
     1621 agcaccattg caatatacgc acgttggagt tgaccgcctg aaagagtgtc aatcgattga
     1681 tccgctattt cttctaaccc aaggtgttcc agtgattcct gaacgatttc ctcgtctttt
     1741 gtcgtcaatt ttcctttcgt ataaggaaat cgtccaaatt ctactaattc tttcacggtg
     1801 atttggacat ttatttcatt cgattgtcgt aggatcgata attttttggc taattcattg
     1861 gatttccaag cctttacttc gttatggtct aaatagattt cacctgtatc ttttggtatc
     1921 aaacgactga tcatcgacaa caccgttgac tttccagcac catttggacc gataaaagca
     1981 gtcaacttct tttcaggaat cgttaactgt acatctttaa ccactgattt tccatggtac
     2041 agtttattga ccttttgtaa atcaatcata ggatactctt cctttcgcga atcaattttg
     2101 tgacaaaata aattcctccg acaaattcaa cgacgacact cactgttgta ttccagccga
     2161 aaatctgctc tactgcgagc tgtcctcctg tcagaaacag catagctacc agaaagcttc
     2221 cgataaatag ctgaccatga cgataagtag aaaagacacg ataactgata tttgcgacaa
     2281 taaaccctaa aaaagtaact ggacccacaa gagccgttga tgtccctgtc aaaagactga
     2341 cacaacataa aatcaaaaat tcaaaacgat ctgtgtctat cccgaggttt actgcttgtt
     2401 catttccgag atggaaaacg tctagcacat gtcgatatcg aaataacagg taccagcagc
     2461 cgataatgat caaagaagca ggcaaaagat actgactctg gatattgcca aagcttgcaa
     2521 ataatctgcc ttgaagcaaa tcatattcat tcggatccat gatgacttgt aaaaaggtac
     2581 tgacactacc aaaaagcgta cctaaaatca tccctgacat cagtaataaa aaaagattcg
     2641 ctctcatttt tttcaaaaga aagaaaaata acaatagact cagcccgacc atcagcccaa
     2701 cgttcaataa gaaaaaaact gtacctgtct gctgaagcat cgtcactcct ccgataaaga
     2761 agaaaaggac cgtttggaca aggacatata acgactcaag tcccaatact gaaggagtca
     2821 aaaactggtt atgcgtaatt gtctgaaagc tgattgtggc actgctgaca gctagcgaga
     2881 caaggataaa tgcaaaaatt ttcttactcc tcaatgccca tgcaaaatgc cagtttccat
     2941 atgtatggat cgtcaagtac attcctgtaa caaataaaac aagaacaagc ataccaataa
     3001 aaatagatgg ctttttttta agcatatcgt cgccccctta tcagaagata aaggaagata
     3061 gccccaccga tgattccaac gatcacactg actggtattt cataaggaaa aatcaacacg
     3121 cgagcgacaa gatcgcaaaa tactaagaac aaactacctg ctaaagcagt tacgccaatt
     3181 gttttgtgca cttgatctcc atatattcta gagacaagat tcggaatgat cactccgata
     3241 aaaggaatgc ttcctaccat gatcaacatg actgcactag ccaatgatac taagccaatg
     3301 cctaaaaact gcatcttctg gtaatctatt cccaaattta ctgcaaacga ttcgcccaga
     3361 ccaatgatcg taaatcggta agcaaatagc caaaccaaga gcaacaatgg aacaacagca
     3421 tacaataact cataatttcc tcgtatcaca gtcgcaaaat ttccttgaag ccatgaagtc
     3481 atattttgta ccaactggaa ttgataagca aaaaaagtag caactgaacc aatgatattc
     3541 cctaacatga ctccaatcaa cggaagcatc aattgatttt tctgggggaa caatcgcgta
     3601 agggataaaa aaagcaatgt tcctgcaaaa gcaaagcaaa atgctgtcaa ggatcggaca
     3661 atcaaggatg caccaggtag gaaaatcata gccactaaaa tacctaaacg cgcactatcc
     3721 atcgtgcccg cagtcgttgg tgaaacaaat ttattttggg tcaaatgttg catgatcaac
     3781 ccacaaatac tactggtcgc tcccacaagg atcaaacaaa tcgttcgtgg caaacgagtc
     3841 actagcaaga tttgtaagct ttgcccatct aactggagca gttctgacaa ttgtacattc
     3901 gatactccga tgaacaatga aatactactt aaaataacta ataatgctac tagtcgtccg
     3961 tagcgcatcc aatccacctt tctaattgag aatgattttc attcttattt taagtgaatc
     4021 gttgaacaaa agcaaccgct ttttcatatg ttttaggtat tattgggaat atgctataat
     4081 cgtaagaaag acaaagagaa gaaggagggt attttttatg gcacaagatg atgtaaaaag
     4141 ccatttggat aagggacttt atggtacacc tttagtgaac cctgaagaac agcataaata
     4201 tttagggaca ttcagggaac gctgctatct tagtatgact gttgctgaaa tgatccaacc
     4261 aaaaaacaaa gaaaactttt taaaagaact caaaaaacat ccagatgctt ccatactctt
     4321 gaatggggct ctaccccttt ctgtccaaaa ccaatatatc cagctagcaa cgaaaaacaa
     4381 tagccgattt actgtagtca acgattacgt cactgcaaat cctgaggaat tcggattgct
     4441 cttaacagca aaagaagcag taaatgaacc tgtgattgat atcgaaaaaa aatacccaga
     4501 agaaaactct tcgccagttg aaaaagaaaa accaaaaaag aaaaaaagct tctgggatcg
     4561 tttattttaa aacgaaaaaa cacataaatt tttacatgca aaaaagcttt tgatcagatt
     4621 gacatcaaat aggacaatct gtcaaaagct ttttctttta aaatacttca ggatataaca
     4681 atttcaatac attcatcgtc aatcttctgc cttttcctgc aaagggataa acatgcatat
     4741 gcatggctgg attgaaattt gcttcgatga tcccatatgc ttttttatca gttgtaggat
     4801 cgatttcttt atctggtata atcaaatcga taccactaat cttagctcct agtgcttcta
     4861 ccgcagaaac ggcgattttt ttgtaagctt cagaaaattc atcagtcata tcgatcgaat
     4921 caccacctgt actgatattt gagttctccc ttagataaac aatttgattg gcttggggaa
     4981 ctgattcgat cgtcaaccct tgttctttca acatcaattg ttctaattca cctaattgga
     5041 tcaattctaa aggtgcacga tgattcgttc cacgcaaagg gtcactattt ttttcttcaa
     5101 caagttcttt cactgttcgg ataccgtcac caataacatt agcagggact cgcaacatga
     5161 tggcttggac ttctccgtcg atcacaaaga aacgatattc cgttcccggc ataaattctt
     5221 ctactaaaac cgaactgtct tcacgaaaag caatggctaa acctgcttga taatcttcca
     5281 gactagctcc ttccttgaaa atcgtgatac ctaacccgta attggttgat ttaggtttga
     5341 tcacaaatgc ctgctcggca aatctcggat aagcttttac cgcttcttcc atggatgaaa
     5401 attctgcgcc tcctggtacc cggaacccag cttcttttaa gacttttttc gttactgttt
     5461 tgttttccat gataagtggg acgatatagc tgtccttact tgtcatgttt gcatttttca
     5521 catattctac atggtcttgg aattgcagac gcaaaaattg atccgactca tctaatactt
     5581 tgacttggac acctttttgg atggcatcga acataaatat ctgagtagaa agttccattt
     5641 cgcgaaaacc ggccaactga tagggacgtt catggctttc tttataatat tgatgtccca
     5701 atgccacagc aacttcttgc tgactcgata gctgtgcttc ttgatagagt ttcgctgcta
     5761 acgtttgttc aggatgatcc atttgagtca atgcttcctc tactaaataa agagaatcca
     5821 accaatccaa ttcaaccagc atttgcttca tcccttcaag aatttctcta ccttcttgat
     5881 gaaattctgt ttgatctgat ggttgttcta gtgctacttt gttatttcga gtcgtacctt
     5941 ctgctaccca ttggtcacag tcttcttttt cttctgtcca taacatgtac atcaggaata
     6001 attgtaagaa ccgaacttgc tctggattga tacccaatct tgcataagga ttcaaatcga
     6061 tattccgcaa ttcaatataa cgaacacctg ttgttgctaa atctgcgact ttctttccgc
     6121 ctcggaaacg aagcggtgta taaaattctt tctcctccga aagtttccct tcttcgatca
     6181 ttcgctcgat atctgctaaa tactgtttca tagaagcata ggaaactttc acatttggat
     6241 gattcgtata gcccagcgca ctgttacgaa tactgcgaac aggttcttgc ggatgtgatt
     6301 catctaaaaa gtagttcttc tcactcattg gtgaagcacc gaataaataa gtgatcatcc
     6361 agcgatagcg cataaagttt ctcgccgttt tcaaataaag ttctgttttg aattcggaaa
     6421 aatcttgaaa ttcttcctga tggctgaaca atgttcttag caactcatcg ccaaattcaa
     6481 aattgaaatg gataccgctt accatctgtt ttcgcttccc gtattctttt gctaaataac
     6541 gtcgatacaa gacatcttcg aaatttttta attttgcaat aatgatcgct tcgtcttttt
     6601 ccggtaaggc aggtggcata cttaatggcc agatcatctc ttctttcggt atagaacgag
     6661 cagtcacatc ataaacagca gcgagatact gaaataattc gggaatagaa tcggtaacag
     6721 gagtgatcat ctctgtttgt gtttcgctaa aatctgtttg gatatagggg tgataggatc
     6781 gatcgccaaa gattgctgga tgatcggttt ttgctagatt tcctgcaaga tcaacccgct
     6841 gtccttcccg ttctatacca aatcgagctt ggtcgataaa agggcgcgca ttgacatgca
     6901 ataataattg cttaaaattc atcattaagt gacctccaat tcatatgaat ctaattataa
     6961 tcaaaaagtc taagaaaaag aacgaaattg tttgagaacc gcttacaaat aagtataaaa
     7021 atgaaggcat caaaaacaca gctatgttaa aaacaatcat tcaaacaagg agctgcaact
     7081 tttgtcacag ctcctgcttt ttagacatgg gttattcttc ttttagtaat cctttgaaca
     7141 atccaacgac aaaatcattt ggatcaaacg gctccaagtc atcgattcct tcaccaagac
     7201 cgaccaattt cactggtaaa tgcaattcat tacggatagc aagtacgata ccgccctttg
     7261 ctgtaccatc taattttgtc aaaacaagac cggtcacatc tgttgtttct ttgaattgtt
     7321 ttgcttgaac catcgcgttt tgaccagttg ttgcatctag tacaagaagt gtttcttggg
     7381 gtgcttctgg taattcccgc tggattactc gttttatttt ttctaattcg ttcatcaagt
     7441 tcactttatt ttgcagtcgg cctgcagtat caactagtaa aacatctgcc tgctctatct
     7501 ttgctctttc caaagcatcg aatacgacag ctgctggatc gccgcctgca ttgccacgaa
     7561 caacttcaac accagcgcgt tcgccccaga caactaactg gtcgattgct ccagcgcgga
     7621 aggtatctgc tgctgccatc aatacttttt taccttcgtt tttgaactga tgggcaagtt
     7681 taccaatact tgttgttttc cctacaccat taacaccgac aaacaaaata actgttagtc
     7741 cgtcttcttg gatatttagt tcatttactt cattgattcc ttccgcttca tagagatcca
     7801 caagtttctc gatgatcgca ttttggactt ctgccgattt tttcgcattt ctgagtttca
     7861 cttcttgacg taatgcttcg gtgattcgta tcgctgtttc aaaacctaca tctgcaccga
     7921 taagtgtttc ttctaattct tcaaaaaagt cttcatcgac tgttcgaaaa ttggcaaaca
     7981 actcattcat ccgttcacca aatgttttac gcgtcttttc taaacccttg tcgtacttct
     8041 cctgtacttc aacttcttct gttttctctt cgccagtaaa agcacgcttg attttatcaa
     8101 aaaatcccat ttgactcccc cctactctaa tacaaatttt tggatcactt tggctacgcc
     8161 attttcttca ttgctggctg tcacgaaatc tgctgcttct ttgacgaaag gcacagcatt
     8221 ctccatcgct acgcctagtc cagcgtattg gatcatcggt aaatcatttt cttcatcacc
     8281 gattgccatg atttcttctt gtttcaaacc aagatctttt gccaataaag aaataccata
     8341 agcttttgta acaccttttg gcataaattc caaaagcata cttctcgttt tgatgatctc
     8401 atacatttca tggaattctg ctggtatttc tttgatttta ggatctaatt cttcttggtc
     8461 ataagcgatt actgccttat taagcacaaa ttcatctgta atatcgctta tatccgctgg
     8521 ttggaattgc agcagcttat tcaacacatt gtacaaggat tgcttccttg gggcactagg
     8581 cagctgtaaa accacattgt cagacaaaac atctagtggc agatccaatt gctgagccag
     8641 tttgtataag cgttggatat ctgatacttc catcactttc ttttccatga tttcaccagt
     8701 atcatttttt tgtaccaaac cgccgttgaa agtgatgctg tagtcacctt catctaccag
     8761 tcctaattct tgcagataat gcaccatcgc agctaagggg cgtcctgtac atagaacgat
     8821 cttgacccct tgctgttttg cttgtgctaa tgcttgttta ttctcatctg aaatcagctt
     8881 ttgttcattt aataatgtac cgtccaaatc gattgctact aatttgatca ttttcgttcc
     8941 tacactttcg tttcgttgat tttcgcctct tcattgatat cttccatccg tacagaaacg
     9001 atttttgata caccggattc ctgcatcgtc actccgtaca gtacatctgc tgcgaccatc
     9061 gttcctttgc ggtgtgtgac tacgataaat tgtgtgtcat cttggaaatc gctcaagtaa
     9121 cgaccaaaac gtttcacgtt ggcttcatct aacgctgctt ctacctcatc taagatacaa
     9181 aacggaacag gacagacacg gataatagaa aagagcaacg cgatggctgt aagtgctcgt
     9241 tctcctccag aaagcaaact taaactttgc agttttttgc caggaggttg tacttcaatc
     9301 tcaatacctg ttttcagcaa atcagttggt tctgtcagta ctaattcagc tttaccgccg
     9361 ccaaacatat ttggaaagac aattttgaat tcttgacgga tagcttcaaa tacttctttg
     9421 aatcttgtcc gcacttcatc atccatctca tccatcgttt caaacaattg gtttttcgct
     9481 gctaacagat catctcgctg tgttgctaag aatgtatgtc tttcactgac ttgttcgtat
     9541 tgttcgattg aatttaagtt tactggtccc agtttttcaa tctgttcttt taaggatgaa
     9601 acctttgttc tactgtcttc gatatcggtt gtttcttgat agtcttggga agctttctca
     9661 aatgtcagct gatattctga ctgcagatag gtcaaatgat tatctaaaat catttcagct
     9721 cggtcttttt ggacttctaa tttggattgt tccgacagtc gtgctttttg ctcacgatta
     9781 cgctctgcca agactgcttc aagcttatcg atctcctttt gccttctatc tcgctgctct
     9841 ttggcttttg ccaactcggc tttcaatgtt tctcgctgag cagataattc gttaatttgt
     9901 ttttccaaac tttcttctgt gacttcatga tcagaaaagt ctgctgtcaa tgtcgccaac
     9961 tgcttttcta tcgcttcttg acgttcggtt gcctctgctt tttggactcg ggcaccgcgg
    10021 agctggatct gcagatgatt aaactgctct ttcaagacag caagatctgc ttgttcttgt
    10081 gctttttgac tttgtacttg tgctcttctg gcttccatct ggtcgctttc ttgacttaac
    10141 gatttgattt cttcgtcgat tttttgtcgt tgcgattcaa gatctgtttg tttgtctttc
    10201 aattcgttcg tttgcttttc gtaatcttcg ataaactgct gcagttcacg tgtctcaaaa
    10261 gttgagattt gcttttcttt ttcaaaacgt tcaagttcat ttatgatgtt ttgcagctga
    10321 tttgtcgctt cttgttcttc aaaacgtaat tgttcgcctc gtgtacgcaa aacttcttgt
    10381 tcttcggcta aacgagctgt ttcttgttgc agctcctgaa cttttttttc ttgtgcttgg
    10441 agttgtttat ctgcttcttc aaattcacta gtcaactgtt tcaattcttg attttgtaca
    10501 aacaaactgc cttggtttcc tcgtttattc gctccacctg tcatggatcc tccggcattc
    10561 atcacatcac cttctaagga aaccactcga tattgatagc ggatcgtttg cgcgattgca
    10621 ttggcactag ttaagtcttt ggctaaaagg atcgttccta gcaggttttg aaccacagtt
    10681 tggatttgat cgggataact gacttgttca ctggcaatcc ctataaaccc ttctacagct
    10741 gctgcttgtg tcaatatatg agccggtaat tgtcttggtt tgattgtcgt cagtggaagg
    10801 aatgttgctc ttcctcctct ttgctgtttc aaatacgtga tggcttgccg agcatctttt
    10861 tcattttcaa cgatgacatg ctgggctgcc ccacctaaag ctgtttcgat agctaacgtg
    10921 aaatctgcag gaacatcgat cagttcagcc actgctccga caatacccga aagctgctgt
    10981 ttatgctgca agaccaatcg aaccccttgg taaaaaccaa aatagttttc ttggatttct
    11041 tgaaggcttt tcttacgagc acgtagctgc tggacttgat tcattaattg atacatttta
    11101 ggctgttctt ttgctaatgc ttcttggaac tttttcccgt tttgttggat catttctaac
    11161 ttctcttttg tttcagttaa agatgatttt agctgcgcct gcttttgtgt caattcttct
    11221 ttttgtaaga tcaatcggtc tactgatgcc tctacttccg attgcttggc taacgtctgc
    11281 ttactttttg ctgtttcttg tacatattga cgttctagat acttcagttc gtttcctaca
    11341 gctgcttgct cttgcatcaa atcaacatat tgatctctta gttctgccag taattctttc
    11401 gttgattttt ggtatttttc taattcttgc tgtgttttta tgagacttgc ttcagctttt
    11461 tggatttctg tttctttttc tgctacttct ttcaacaagc tttcttgaag cttttcaaaa
    11521 tgtttaacct ttttttgtgc ttctgctaat gaagtttgat attcttgcga gcttttttgt
    11581 gtatgctttg tccgctcctg caaaacatct ttttgtcctt ctgtctgctt cagtttttcc
    11641 gaaagatcta ataatacttg ctgatttttt tcgatcagac gatcggcttg cgcattttct
    11701 ttacgctgct tcgccaaaat ggattcttgt tcttggatcg actcagatag tttgcctaat
    11761 tctagattga atttcgcaag ctgtgcttgt ttgttgtccc aatctttttt tgctgtcttg
    11821 atttcggcaa ccatcaaaga gacatcggtt tgagtcagtg tttcttttag ccgtaaaaac
    11881 tcctttgctg cctcactctg ggcggccaaa ggagttaact gttcttccaa ttcgtggatg
    11941 atgtcttgga cgcggctgag gttgtcttct gtttcaaaaa gcttctgttc tgctttcttt
    12001 ttccgctgct tgtatttcaa gacaccagct gcttcttcaa agatcccacg acggtcttcc
    12061 ggtttactac tgaaaatcgc ttctactttt ccttgagaaa tgattgagaa cgattctttt
    12121 cctaatccag agtctaagaa caattcttgg atatctttta atctgcagct ttgtttattg
    12181 atgaaaaact cgctttcccc agtccgtcga taacggcggg ttacactgat ctcattgtac
    12241 tccaatggca gataatgatc actattgtct aaaatcactg tgacttctgc aatattcagc
    12301 tgcttacggg tatcggaacc ggcaaagatg atatccggca tttttccgcc tcgcagactt
    12361 ttagcagatt gttctcctaa tacccagcgg atagcttctg taatattgct ttttccactg
    12421 ccatttggtc caaccactgc tgtgacgcta ttttcaaagt caatgactgt cttgtctgca
    12481 aatgatttaa aaccggctat ttcaattcgt ttcaaataca aagctttccc tcacttactt
    12541 ttacagtgct gccaatgcat tttccgccgc agcttgttct gctaattttt tcgattttcc
    12601 ttgtccagtg ccaatcaatt gatcatctac ataaacttcg atccaaaata ctcgttcatg
    12661 agctggacct tcttcattga tcaatcggta ttcgattgat acatcacctg atttttgcaa
    12721 gacttcttgt agttttgttt tatgatccat ctcatgtgaa aaagcacctg ctttgacttt
    12781 aggaaaaatc actttttcga tgaatgcatg ggtcgtgtcg aatccctggt caagatataa
    12841 cgcgcctaaa aaagcttcga ataggtcgca aagcaatgct ggtcgcgtac gtcctcctga
    12901 attctcttct cccttgccca acaaaacata ttgatcaaag tgacattctt tggcaaattt
    12961 cgacaagctg tcttcgcgaa cgatcgcagc tcgtgttttt gtcaattttc cttctggcaa
    13021 atgaggatat aaacggaata aatactgaga aaccatcagt tctaaaacgg catctcctaa
    13081 aaattcaagt cgctcattat ccgataattg cagattgcga tgctcattca cataggatga
    13141 atgggtaaaa gcttgttcaa gcaaatttag atcgtgaaaa acgatgttat agttttcttt
    13201 tagttctttt gtcagctggt tgtccatttt ccacattcct tttatggata ttttttcgat
    13261 acttccttat tatactggtt ttttgtcaaa tttgaaatgg ttcggtcaaa aggtaagtaa
    13321 agctttaata atcctagtga ttgacgagaa gttgtgaaaa attcgtttag tttcgagtat
    13381 gaagaaaaaa ttttgtgaaa atggctttcc atatttttgc ataaatttga cttaataccg
    13441 agaaactgga ctttgaacac cgtttattgg agaagtcatg aaatcagcgc cttgacctga
    13501 atattagaga aataaacaga aaaatatcaa aaaaccgaaa aaggacatcg ccctttttcg
    13561 gtcattttta gtgattatga acgtgaatct gctgtaacag aaacatttgc ccatgggttt
    13621 ggtacatcat aagaccaatc ccagtctttg acacggtcgt tgactggcaa tacttcatta
    13681 cggaacaacg ttggaatcgc aaatgcttct tcaaatgcat attgttgcca tttttgatat
    13741 gcttcttttt ggtactcttc atcaaatgct ttgcttgaac tgatatcgtt gatcaatttt
    13801 gtattttctt ctgattcata acgagtgtag ttgaacgcag catttggtcc ccatagtcca
    13861 cttggatttg gatcagttcc tgtattccaa gcagcttggt aaacatcaat ttcaggatca
    13921 tcattttcta atttatcata gaaagaattg aattcgatca gacgacctgt tgtgtattgg
    13981 acatccaaac cgatttcttt ccactgttgg atatagtaat ctgccaatgg ttgcgctgtt
    14041 tccccaccgg acatcgaagc aaagttgatt gttagtttat ttccatcttt gtcttctctt
    14101 aaaccatcgt catccacatc tttgtagcca gcatcatcta atagtttttt cgctttgtct
    14161 agatcttgtg tgaatccttt catctcatca tcatggtatg actggaatac tggaggaatc
    14221 aatgaagtcg cattgcttct caaacctgca tagaaacgct cacctactgc attattatct
    14281 aatgcatagc ccattgcttg acgtaatgat ttatcagcca tttttgaatc tggattgtat
    14341 tccacactct ttgtatctgc attccattta cctaacttga atcctagata agtataagaa
    14401 gtttgttcac ggcctaagtt tgtatagcca gcaatttctt tataggtatc gtaagtatct
    14461 gttggcattt ggaaaatcat atcatataat ttagagttca atgcttcagt tgcacttgcc
    14521 atcggtacag atttcatcgt gatctttttc aatttaggcg taccattcca gtagtgctcg
    14581 tttggcaagt attctactga ttctcctgca acgatattac tcatgtaata agggccaaaa
    14641 gtcactggat ttttacgtac ttgatcgctt gattccatat ctttgattgc tacaccttct
    14701 aacgtatgtt ttggtgctgc atatgaccaa ataccaccac ccgattgaag catgcttggg
    14761 tttacttctt tatattcgat ttcaactgtt ttgtcgtcta tttttttgat tccggagatt
    14821 gtgtcggcac ttccatcatg atattcttcc atacctacga tgttacggaa agtgtcgtca
    14881 taacggatac cagtgtaatc tttattccca atgatttcgt aagggaaaat cacatcgtcc
    14941 gccgttacat cttcaccatc tgaccatttg acgttgtctt ttactttgat tgtcgctttt
    15001 ttcgtatctt catctagatc aagcgttgca gctccgtcgt ctgtgattct gaaatcgtca
    15061 tcgtttgcaa atagcggttc atgagaagga gccataaaag ctgcatcata tgaatcttgg
    15121 taaaactccc actggaacaa tcctttaaac tgactgtccg ttgcgaccgc agcttctaat
    15181 tcgccgtctt cgattgcatc atcattattt tttgttgcaa ttggaaaagt taattcctct
    15241 ttgttattgc cgttgcctcc agagttgttt ccagaggtat ctgtacctcc gcccccgcat
    15301 gctgcaagaa cgacagcaga catcagcgct gcaaatccaa aaatcgtttt cttcattcct
    15361 atttccccct tcgcttctat actagcctaa ccgttggcgc gcatctgcag aacgcttgaa
    15421 tgcttgtcca acataattga tactcaacat caaaactaaa ataaggattg atgcgggtaa
    15481 ccagatccat tgccgattca ctaagacttc tccgttactt gcataaccga tcaatgtacc
    15541 taaactaggt acatttgccg gcaagccgaa acctaagaaa gtcaaagttg tctcgatacc
    15601 gatgtttgcc gcaaagttca tcgtcaaatt agtaatgatt aatgaactta gattcggcat
    15661 gatttcgcgg aacatgattt taaaatcatt ggttcccatt gtttttgagg cactgacata
    15721 atctcttctt gcttcagata atgtcttgct tcggaataat cgggctttag ctacccaata
    15781 aaatgcactc atgatgaata taaaggacca gacattgtat ctggaaataa tcgatacgaa
    15841 gacgataatg atcagcatga tcggtagaat catgataaaa tcaacgatcc gcatcaagat
    15901 gttgtcaatg atccctccgt agtagccaga gatgatccct aatccaaccc cgatgatcga
    15961 agtaaggatc gtgatcgcaa atccgatcgt aacagagttt ctagcaccta tgatcaattg
    16021 tcctaataca tcacgtccgc cttcatctgc tcccagaatg aacttttgtc cttgtgtcga
    16081 aactgttcct ggtgcagcgt atttgtcgaa gatactaaca tacatcactt tgtcttgatc
    16141 ggtcagcatc gcaccgataa agatcactaa taataaaacc actagcaaac ctaatgaaaa
    16201 aatagcaatt ttatcttttt taaattctcg agcaatcatt cgtatcccca taggaggaat
    16261 actttcttga actgctgtat tgtttttttc actcatcgat ccattctcct ttctgattgg
    16321 ttttattgaa tccgaatacg cgggtcaacg atactcatga tgatatctga aagtaatgtc
    16381 cccactagcg tcgcgattcc taaaatcaat actaaggatg tgatcacggt atagtcccgt
    16441 tggccgatgg aatcaataaa tagttttccg actcccggat aaccaaaaat cttttcgata
    16501 actacagaac cagcaatcaa cgaagtgatt tcatacccca tttgagaagc catcggcaac
    16561 gctgcatttc ggaaaatatg tcgactgaat actttattcg tcggtacccc tttggaacgg
    16621 gctgttctga caaaatcgag cgatttcgaa tcgattactt cacttcgcag atattggatt
    16681 gtgacggcag tcgccaaaaa tgcttgggta attgatggta acagaatata tttcaaacgg
    16741 tctatccatt gagtgataaa tccaccttgc aaacctagtg tttgtgatcc acttgttggg
    16801 aaccagccta aacgataacc aaaaacaaat agcatgatca agccgaagat aaataaagga
    16861 acagcaaaac taaagaagtt gtagattacg acaatcttat ccaagattga attttgatac
    16921 cggccagcaa gcatccccag aggcacagca atcaaataag taaggacaac tgtcaagatc
    16981 gatagatata aagtgttgat gatccgttcg ccgatcaagt cagcaacaga gcgtttgtat
    17041 aagaaacttt gaccaaaatc gccttgcaaa gcatttccga tccaacggaa gtactggaca
    17101 taccatgggt cattcaagcc agctgcttgt cgcatctgtt cgatcactgc cggatcctga
    17161 tttggattga tcaaaccggt aaatggatcc cccggcatcg cttgggctaa taagaagatc
    17221 agaacactta aaataataac ttgcgggatc atcaataaaa cacgtcgcag aatcgttttc
    17281 cacattagct tgcacctcca tctttcaacg ccactttgtg tgtagttgtg atcgtacgca
    17341 aatcgtagac tcgaccagtc gcatcatagt attctttttg gttttggata tactcttgtt
    17401 ctactcttcg tcgattttct ttgtggcttt ccctattttc tacatcgatt tgcggaatag
    17461 ctgaaagcag acgtttcgta tagatatgtc gcgggtcatt ataaatgtct tctcttgtac
    17521 cgatttctac aaatcgacct ttgtacataa ttgcgatatt gtcgcacatg tgttttacta
    17581 cacctaaatc atgtgaaatg aataaatagc tgaggccgta ttcttgctgg attcgtttca
    17641 tgaaattcaa tacttgcgct tgaacagata gatccagagc agatacaggt tcatcggcta
    17701 cgatcagttt tggattggta gcaacagcac gggctactcc tagacgttga cgttgtccgc
    17761 ctgaaaattc atgaggatat ttatatagcg catcttccgg catcccaacg atatctagca
    17821 gtccttttac ctttttcttt tcttcttgat cgcttaaccg ttcaaaattt cggatgggtt
    17881 cagcaatgat atccaacact cgctttttag gattcaaact ggacattgaa tcttggaaga
    17941 tcatctgcac atccttgtta taacccattt ttttgcggtt gcttcgcttc gttacatctt
    18001 ttccttcata catgatttgt ccggctgtca ccttttctaa tcccacaacg gcttttcccg
    18061 tagtagattt tccagagcca gattctccca ctaaaccata tgtctttcct ttttcgataa
    18121 taaaatccac accatctaca gcatatacat gatccgttac tcgattgaag aatccactgc
    18181 gaatagggta atgaactttc aggtctttga ttgtgattaa ttctgtcatc tacacctctc
    18241 ctttttcgtc tctaaagtgg aaatgcttat agcaggagca acgtaccaag tggttcggtg
    18301 cgacttcatg aaggataggc tcttcttcat gatcgcttgc tggtatccat ggaatacgtg
    18361 gtgcaaaccg acaaccttta cgaggcatgt ttttcagaga aggaacgaca ccttcgatca
    18421 catggagttc tgcatcatga tttccttctt gtggtataga gttcagtaaa gagcgtgtat
    18481 aaggatgctt aggatgatca aacagttctt ttacacctgc tacttcaacg aactgtccgc
    18541 catacatcac tgccactcga tcagccattt ctgctacgac tcctaagtca tgagtgatca
    18601 agatgatccc tgtttttgtt tcttcctgaa ggtctttcag taaatcaaga atctgtgctt
    18661 ggatggttac atctagggct gtcgtcggtt catctgcaat gacgattgga ggtttgcagg
    18721 cgattgcgat tgcgatgatg acacgttgtc tcataccgcc tgatagttca tggggatatt
    18781 ggcgtccaac tctcgcagga ttaggaatcc ctacttgttc tagcaattct agtgcacgag
    18841 cttgacgctg ttctgcattc atctttgtgt gataggaaag tccttctttg atctggtctt
    18901 cgattctcat gagaggattt aatgcagaca acggatcttg gaaaatcatt ccgatatcat
    18961 ttccgcggat tttattatag agcgtttcgt tcaggtcgat taaattcaga tcgttatata
    19021 aaatctcacc tgtaattttt gtgttattag gatcaagtaa tcccataatc gtagtagcca
    19081 aggtactttt cccgcaccca gattccccaa cgattgctaa aatctcgttt ttgtctaaag
    19141 taaatgaaac gtcatctact gcatcgtaat agtcatcttt gagtcggaat cctgtatgta
    19201 ggttctgaac ttctaataat tggttatcgg acaatcgaat acccctctct gcttcctaaa
    19261 tggaagtttt tcttaataaa aaaggacatt acacagaata aagcgtttta taacaaaggt
    19321 tattcttatc tttgtgtaat gaattattaa taattatata gaaaatcgct ttactttgca
    19381 acataatttc tgtttatcgc cagaattatc agaaaaatga cttttacgtg tcaaaaagat
    19441 taaaaaaaga aggataaaaa gctgtatgca gctttttatc cttctttgct tttatttgaa
    19501 attatttaag atggctgctg atataatcta ctgcgccacc gactgtttca atttgttcag
    19561 cgtcttcatc tgaaatttcc gtaccgaatt catcttcaag ctctaaaacg aattccataa
    19621 tactaattga atcagctttt aaatcatctt tgatgttcaa ttcatccgtc actttatcgg
    19681 tatctatctc aaaatgattt gaaataattt tcgctacttt gttaaatact tcttcgcgag
    19741 tcaacttttt cacctccaag aatcatacac ttgtcaatta cctacaccat ggctaatttt
    19801 actggcattt ttcaggaatg tccacctttt tttcaatgga acggattgct tcagatatcc
    19861 aaatactatt cttcgctttt ttcaaattgt tcgactaatt gtccaactac atctgtctca
    19921 agcattgtgt ggatttggcg gatcgtatag cgaacagcat cggggcctgt tgctccatga
    19981 gttttaatga ctggtgcttt caagccaaac aaaacggcac cgccatgttt ggaataatcc
    20041 atttctgctt tcaaagaacg taagccgtct tttaaaagca tagcgcccat ttttcctttg
    20101 atcccttcat ttagaatagc agattttaat agattcatca tattcattgc tgttccttca
    20161 atagatttca aaacagcgtt gcctgtaaat ccatctgtga ccactacatc agccacacca
    20221 ttgagtagct cacgagcttc tacgtttcca ataaaattca agcttgtttc attctgaagg
    20281 agttcatagg cttttttcgt cacttcacta ccttttgttg cttctgttcc gttgtttaag
    20341 agtgcgacac gaggacgtcc gatacctcgg actttacgtg cataaaaaga acctaaaata
    20401 ccgtattgca acaaatgttc tggtttgttt tctgcgttag cgccaagatc cagcatatcg
    20461 aaaccggcac cttctttccc tactactggc aaggtagaca tcaagccagg gcgttcgata
    20521 tttttgatac gtcctacgat gaacaaccct gctgctagta gcgcgcctgt attccctgct
    20581 gaaaatacag catctgcttc accgtttttc actgcttgtg cagcaaggac cattgatgca
    20641 gtcttttttc gtcgaattgc ttttactggc tcatcatcac tattgatttt ttcgtctgta
    20701 tggataatcg tgatgttttt ttcatttgtt aagtatttct tgatttctgc ttcttttccg
    20761 taaagtaaga attctacttc tggaaaatct tgctgcgcta aagccacgcc ttctacaatt
    20821 gcttgaggtg cgtgatctcc gcccattgca tctacggcaa ttttcattgt ttccactcct
    20881 ttttccgtca tcagtataaa gtatagcaca gtctgctaat cgaaataacg attatcctga
    20941 ttttctattt ggaggctttg cgctaaaggc tgatactcag gaagcatctg ccaatccttt
    21001 atctgccaaa gggctgcagc ctcttctctg gctacctcca aaacatttcc atccgtcacc
    21061 aagtcagcaa tcgcaaattg aggcagacca gattgacgga agccaaatac ttcaccagga
    21121 ccacgtaatt ccagatcttt ttcgcttagt acaaaaccat tggtggtctc tgtcatgatt
    21181 ttcatccgtt ccacacctag ttcattttta ggattagcaa ctaagataca gtaggaagca
    21241 tcacttccgc gtccaacacg tccacgaagt tgatgcagct gtgccaatcc aaatcgatcc
    21301 gcatccatga tcagcatgac ggtcgcgttc ggcacgttta cccctacttc aattacagta
    21361 gtcgaaacta atatctgcat ctggttgtct ttgaaggctt ccatgattac ttctttttcc
    21421 tgattcttca ttttaccatg taacaagcca acttcatatt ttggtgcaaa tagttcacga
    21481 agtttttcat agatttccac tgcatttttt acatctaatg cttctgattc ttcaatcagc
    21541 ggacagatga catacatttg atgtcctctt gccagttctt ttttcgtcca atccagtaca
    21601 ctgtccaatt gaggtgttct tacccagcgt gtttcgatcg ggatacgtcc agctggaagt
    21661 tcatcaatga tagatacatc catctcacca tatgctgtaa tagctaatgt tctaggaatc
    21721 ggagtcgctg tcataaataa gacatctggt cgccagcctt tttcacgaag tactttccgc
    21781 tggttcacgc caaaacggtg ctgctcatcc gtaataacta gacctaaatt ctgaaattcg
    21841 actccatctt ggatcaatgc atgtgtgccg atcacgatgt taatttcccc ttgctctagt
    21901 ttttcaagga gctcacgacg ttcttttgct ttcgttgacc ccgtaagcaa tgcgatggtt
    21961 acttcattgg gatcaaacag ctgttgcaag ctttccatat gttgttgtgc caagatttct
    22021 gtaggcacca tcaacgcacc ttggaaacct gctgtaacgg tagcgtataa cgcaatcgcg
    22081 gcaaccactg tttttccgct acctacatct ccctgcaaaa gtcgctgcat atgctgcgga
    22141 ctgcgaagat cccgacagat ttcattcgtt acacgttttt gtgcatttgt cagttcaaaa
    22201 ggtagctttt gagtaaattc tttcaatttt tggacatcat aaaggatctc caaaccattc
    22261 gtttctgctt tttctgcttt tttcaatcct tgaagacgca tttgaaataa aaagaactct
    22321 tcaaaaacga cacgtcgttt ggcttgatgg ttttcttcgt ggtcttttgg aaaatgcata
    22381 cttcttacag cagaagctcg gtcaagcagt cgatatttct ccacaaggtt gtttggcaga
    22441 ttttcttcaa tcaatgtgcc gtattcttga aacccttttt tgattagatc aactaatgtc
    22501 gtttgacgaa ttgatttgtt gacgtgatat atcggcgaaa agtcatccat tgtttgagaa
    22561 cctaaaatct tcatcccgtt caatgcttta cgctttgcat cccatttacc gtatacggcg
    22621 atttcctccg ataaaatgac tttttctttt agataaggtt gattaaaaaa agaaacgttg
    22681 aacacatcat gttcttgcat catacgaaat tgcaatcggc tttttttata accaaatcga
    22741 ttcatcacag gaggcgagac tacgattcct ttcaaagtaa ccttttcttg atcttggatc
    22801 tcgtttagat ttctttcctg aatatcttcg tagcggaaag gataataagt cagtaagtct
    22861 tcaatcgttt ggatgcccaa gctggcaaga ctagctgctc ttttttctcc gactcctgcc
    22921 agcatagtga ttggatcatc taaagaacgc ataaattttt cctccctccc ttaacaaaaa
    22981 aaggacttgg tcagcgatca tacctgatca ctaaccgaag gccttttatg aatgaatact
    23041 gtgttagaaa gatattctga cacagaaatt attcagcaga aaatagataa ggatagacag
    23101 gctgatctcc ttggtggatc tcgatttcca aatcaggatg tttttctgtt agtttctcag
    23161 caaattcatt tgcttgttca actgttcctt catcacctgc caagatcgtt acgatctctg
    23221 tgtcctcatt gatcatttgt tctagtgtac ttaacgctgt tgctgtgata tctgggttag
    23281 agatcaggat ttttccatcg atcatgccca aaaagtctcc ttctttgatc tctacaccgt
    23341 caattgaggt atctctgatc gcgtgagtga cttgaccgct cacaacgcta gacaacattt
    23401 ctgtcatcgt ttctttgttt tcttctaaag aagcttgatc attgaatgaa agcatagctg
    23461 tcaatccttg ggaaatcgtt cttgaaggaa caactgctac tgggatatct gctacttctg
    23521 ctgcttgatc agctgccatg aagatgttct tattgtttgg caagacaatc actttatctg
    23581 cgtttacttc ttgaatcgct ttaacaatgt cttccgtact tggattcatt gtttgcccac
    23641 cactgataac ataattagcg cctaaacttt caaacagctc ttggacgcct ttaccagcag
    23701 cgatcgctac gataccatac gggatacgtg gtgtttcttt tttcgcaaac tcgttcactt
    23761 cttcatcgtg ttccaaaatc gtctcatgct gcaagcgcat gttgtcgact ttgattttga
    23821 ctaatgaacc gaatttttgt ccataattca agacttctcc tgggttttct gtatgcacgt
    23881 ggactttgat gatctcgtca tcattgacca caagtaaaga atctcctata ccatctaaat
    23941 agttacggaa ttcttcgtaa tcgaatgtac tatcgactgt aggtccttct ccgattttta
    24001 ccatgatttc tgtacagtaa ccaaaatgga tatcttctgt cgccatttga ccttggacac
    24061 tgcgatgatg ttctgcattg accatatcat ccatttctgc tggagaaggt tcatatgttt
    24121 catccgcttg gaattctcca ttcaatgcac ttaaaaagcc ctcataaaca aacagcagtc
    24181 cttgccctcc actgtctacg acaccgactt cttttaggac tggaagcaag tctggagttt
    24241 tagctaaagc acgctttgcc ccttttacaa cagctgtcat tacttcaacg acatcatcag
    24301 tactttttgc tttcttttct ccagctttcg ctgcttctct agctactgtc aaaatcgttc
    24361 cttctactgg tttcattact gctttatacg cagtttcaac tccatgagtg aaggctgcag
    24421 caagatcgga agcatttaat gagacaacat ctggaatttg cttagaaaat ccacgaaaca
    24481 attgagacaa aataacgccg gaatttccgc gcgcacccat cagcaatcct tttgaaagca
    24541 ttgctgctaa ttctcctact ttttctgagg ttgaatctac tactgcctta gctccactag
    24601 tcatagatag attcatattt gttcccgtat cgccatctgg cacagggaat acatttaatg
    24661 aattgacgta ttctgcattt acttgcagac gattcgcacc tgcctggacc atttcctgga
    24721 actggcttgc acttatttct gttacactca cctagataat cctccttcag atcgactgct
    24781 gagttaccag ccagtctcct gcctagtctg gcagtacacg aacaccttgt acaaagacat
    24841 taactgaatt ggcagttacg cctaataatg cctctagatt gtatttcact ttttcttgga
    24901 cgttgcggga aacttcagag attttcgtac catagctgat aatcgtataa acatctacag
    24961 caataccatt atcttcttgg cggacaacta ctcctcttga gtaattttct ttacgcaaaa
    25021 tctcagtaat attatcttta atttgatttt tgctggccat tcctacaaca ccgaacacat
    25081 ctgtcgcggc gccaccaaca actgttgcaa ttacatcatt ggtaatttca atggttccag
    25141 ctgacgtttg aatttttaca gccatgaatg agcctcctta acgggtaaaa aactcccatt
    25201 tttcttctat catattttat catagcgcaa cttgaaataa aaggcagtta cccaaagtac
    25261 tcaaaaacct tgcaaaattt caggtataac cttgtttaat ttacaattat tcttctcaat
    25321 tgtcaattgt atctatgaaa atgtacttgc aaatttccat tctatatgat atgttagtct
    25381 ggtatgagct gaattattct attgctccaa atcaaggcaa aggaggaaat taccatggca
    25441 aaagtttgtt actttacagg tcgcaagaca aaaagtggaa acaaccgttc acacgctatg
    25501 aactcaacaa aacgtactgt taaacctaac ttacaaaaag ttcgcgttat ggtcgacgga
    25561 aaacctaaaa aagtttgggt gtcaactcgt gctttgaaat caggaaaagt tgaacgcgtt
    25621 taattgaatg atacttggac cgttcagata gtgaacggtt ttttatttaa taaaaatttt
    25681 taagggtggg acttttgatt cctacccttt ttttgcgttt atttttgtta tacgaacaaa
    25741 ttatgaagtt atttattcta aattttattt ttttgtgaaa attagactct ttttaagcag
    25801 agaaaaacaa ggatatgata aactgaatac aacttcatag aaatttggtg gaaaataaat
    25861 atgaaaaaaa atttaaagtc attctttaga gaaaacggac gctacgcagc agctagtttc
    25921 ctgattccgt ttttgatttt agcacttgtt tatctaagta tcgggatcta tcctggaagc
    25981 agcagaagta ttttggcaag tgatgccttt tcacagtttt ctaattttca tgccagcttc
    26041 cgaaatgttc ttcttggaaa gcaaagtatt ttttatacat ggaatgcttc tctaggattg
    26101 aattatttat ctttaatttc ttactatcta ggcgggatct tcacaccact ggtgttcttt
    26161 tttccaaacc agatgatgcc agatgcactg tatgttctga ctttgcttaa ggtcggctgt
    26221 gccggattgg ctttttggtt tcttgcacgt acgttcaaga ttcctcgctg ggggcatgtt
    26281 gcacttagcg tcagttacgc attgatctca tttatcactg cacattcgga gatcatcatg
    26341 tggctggatg catttgttta tcttccatta gtgattttgg ggattcatcg tgttatggat
    26401 cttaaacggc cgaaactttt atttatcagt tatttactct tgtttttatc cagtttttat
    26461 atgggtttta tgattggcgt attttctttc ttatatttta tcgcccgtct attgacaaac
    26521 tggtcagaat ataaaaaaac gattgttcct tatggtatca cttctttact ggcaggcggc
    26581 gcttcaatga tcattgtatt gccagcagtg ctggatcttc ggtcaaatgg cgaggcactg
    26641 agtcagatca ctacgttgaa aacagaagcg acttcttatc tggatctaat catgaaaaat
    26701 atgattggtg tttatgacac gacaaaatac ggatcgattc cgttcgttta cgcaggtctg
    26761 ctaccattag tcctctgtct gttttatttt gccagtcgga aagtaccgtt gaaaaataaa
    26821 tttttgtacg gcagtctttt cgccatactt atcgcaagtt tctatcttgt tccattgaat
    26881 ttgttctggc atggtatgca tgcaccgaat atgtttttat tccgttatgc ctatctgttc
    26941 tctttcttag tgctcctgct tgcaggaaaa ggatgggaaa agatagaaca agacgatcgt
    27001 gggctgttgt taggaatcat tttggtcgta gctgctctct ttgcggtggc atggggcatc
    27061 aaacctgaag gaagctacac ctatgtgacg atgacttcct ttgtattgac agtggttttc
    27121 ttagggattt atgcttttgc tgtgggattt tatcagcata aaaaactttc attcaatcat
    27181 ctgagtatat tgttattatt gatcatgtct gctgaagcac tggtcaatac gaacagtatg
    27241 atccatggca ttcttgatga ttggaattat gcttcccgca gtttatatac tgatccttat
    27301 cctgacttga aagctcttgt aaatgaaaca aaaaaagatt ccgactcgtt ttaccgcttg
    27361 gaaaatctgg atccagtatc tgcaaacgat tcgatcaact atggttacag tggtatcagt
    27421 ttgttttctt ctataagaaa tcgtaattct tcctcttacc tggataaatt aggtttccgt
    27481 tcaagaggga caaatttgaa tatccgttat aacaataata ctttgctaat ggatgggttt
    27541 acaggaatca aatataacat ttctaaaaac gaaagcacgt tcagtaaata tggcttccgc
    27601 caagctggtc aaagtggcga ctatacactg tatcagaatg tcaatgcatt gccactagca
    27661 tttactgcac cgttgtccgt taatgatgtt gagcagccag tgacagataa tttgaccagt
    27721 cagacaaact tgatcaatac attgtctggt ttaaatgaac agtattttac attctataca
    27781 cctactttga agaatcagtt gaatacgacg atcacaaaga caacgactgg cgtcacttac
    27841 tcagaaacag cagataacca gccaaaagag ctcacttggg aagtccatgt gcctgcaaat
    27901 acacaagctt acttaagttt gttcccaacc aattttgcac aattagaaag ttctagtgcg
    27961 acgatcactg tcaatggtac atcgcgaaaa acgcaaatca acatttctgg tcaatactat
    28021 gatcttggct attatcctca agagacaaca gtgacattta ctgcaagttt ttatggaacg
    28081 aaagaagtaa gtttcatgga gcctaaagtc gttggtctag atgttgtagc ctatcaagcc
    28141 gcaatgaatc agatcaaaga aaaaggtgtc gaaatggaag ccactggcag aaaagcttct
    28201 gggactgtaa ataccgctga agattccatg ttggtgacaa ctattcctta tgacaaagga
    28261 tggaaagcta aaattgatgg gaaaccagtc aaggtcagca atttcaaaga cgcattcttg
    28321 atgataaaag ttccagcagg taaacatact gtcactttca gttacttgcc tgaaggattt
    28381 acgataggtg caatcctttt tgtcctatgt attgcattgt ttattcttta tgtctggtac
    28441 cttgaccgca atcgcccctt ttatggcacg attgatccag caatagaaga aaaaccgcga
    28501 aatacacaca gaagaagacg aagaaaataa agaaacgatg gaatctgtgt cactcttcta
    28561 tttaaatggc aaaaatttca atctttaaat gacaaaaacc acctgatttt tggtggtttt
    28621 ttatatacct atgactaaac tatctcttgc tgcttcgaca acttctacag tgatcttatc
    28681 catttgattg attcttaaaa tccgatcaat ctgcgcaaat aaacgatgaa taagcctaaa
    28741 atttcctttt gtgatcttaa tgatagttgt gatagcctca taatcagaaa aatcttctag
    28801 cttgatatca attcctaaat ctgcccattt gtattccaaa atatgatgtg tttcgtcctt
    28861 actaaggtta tcaaattcat gtgcaaatcc tatgcgagag taaagctgcg gataacgaga
    28921 caatcttttt tcaataccag gcataccgat aagaaccatt gccagatcat ttctatcgta
    28981 aatatctctt agctgttcta aatgctgaag ctttaaacga tcaatttcat cgacaataat
    29041 taagtcaatt gcttcatagg ttttaggcac aggaggatct tcttgattgt attttagtac
    29101 catatgcatt gctttagtca gacctattct actagttatt gtgttaatat ccgaactgac
    29161 acgagacatc ttttcagctg gtacagtata aaaaacttta tttacactta atatttcatc
    29221 tgttgcattt atcaagaaca agaaaaagtc tgaatattca aaaaataacg gtgtacattt
    29281 ttaaaaggag atgtataatc gagatttaga aagcgttttc ctaaaaattt aatgaattag
    29341 gaagtgatta ctatgaaaaa attatttagc gcacttttta ttctgttatt tggctttatt
    29401 ggtattggga cttctggaaa tatagttaaa gctagcacga tggttgaaca aacaagtgaa
    29461 atggcaaaaa cttttagtca attaacacca gaagaacaaa gttacttcaa atccaaagga
    29521 tttagtgata aagatacttt ttacactcaa aagatagagc ctcaagatcc gaaaacacgt
    29581 ttttctgcaa atgtattgcg gttaaccgca tctactaaaa aaattactaa tacgcagggg
    29641 cggacagaat atattataac aagtcccaat tcggcgatgt atcgtatttc tacacgcttg
    29701 agtttaggta gtgcaaaatc atatactcaa acagtaaatc tagctaattc ttaaggttat
    29761 ggtggaggaa tgtattttac atttactggg aaaagacaat attacagtgt aaaattagct
    29821 atgcaagttt ataatacatt tggtgtaggg tcagtctctt gcactgcggg aggattgaca
    29881 ataggaaaat gataatttct tactatgtct taaatagaag tgatgatgaa ggactcttcg
    29941 tttttcaatc attacatgag gatacgatgc taaaagagtt ttataatcgg attaagaata
    30001 aacagataga aacaaattta tattttttaa gtgactctat cttgcctttt attagcaacc
    30061 ctaaaattct taacttgttg cagacaaaag atttgaaaag tttaccgatt attgtcattg
    30121 atgaggaaat tgtaaaatat ggcgcttatc taagcgtaga agaattagaa aaattaacta
    30181 acattggaat gagtattcag gaggataatg gggtacatgg aacatagaat gaatatgcac
    30241 agttgagaac ggaatttgac atttatttag accaacaggg tataccctgt tggtcttttt
    30301 gtgcgttcat gatatacttg gtgtcatttt tatatttaaa tggcacaaat atttctaatt
    30361 atttgtcatt taacgctgaa aatttttgct atttatctaa aaaagtgaca ggatactcat
    30421 ttggccttca tagaattttc taaatatgaa tcatcaatga atttaattaa aaaaataagt
    30481 agggcaaaat ataaatgttt ttgccttact tgtttccatc agtttagttt taaattgata
    30541 agttatatag ttatggttga atgtgtgtct cgcatattag tatttttacc atctgctagt
    30601 tttattatta atctataaca atagataatt tataattcgc atattcatta agctgttcca
    30661 aaaagctgtt cattgatttt tttgaagtaa atctacattc caaaagataa cagccccctc
    30721 cactaatctt ataatttcta atcaagaatt tttcatgatt tttaat
//
LOCUS       DACEUZ010000017        30755 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.017, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000017 DACEUZ010000000
VERSION     DACEUZ010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30755
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.017"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(48..1112)
                     /locus_tag="IXG44_000689"
     CDS             complement(48..1112)
                     /locus_tag="IXG44_000689"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HAQ1657428.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKDPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAQEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(1109..2194)
                     /locus_tag="IXG44_000690"
     CDS             complement(1109..2194)
                     /locus_tag="IXG44_000690"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HAQ1657429.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            complement(2207..3184)
                     /gene="mvaD"
                     /locus_tag="IXG44_000691"
     CDS             complement(2207..3184)
                     /gene="mvaD"
                     /locus_tag="IXG44_000691"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HAQ1657430.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKKGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            complement(3177..4121)
                     /gene="mvk"
                     /locus_tag="IXG44_000692"
     CDS             complement(3177..4121)
                     /gene="mvk"
                     /locus_tag="IXG44_000692"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HAQ1657431.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            4443..5096
                     /locus_tag="IXG44_000693"
     CDS             4443..5096
                     /locus_tag="IXG44_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAQ1657432.1"
                     /translation="MIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFYAN
                     PEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEVRV
                     YDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYDYY
                     KELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVSI"
     gene            5181..6086
                     /locus_tag="IXG44_000694"
     CDS             5181..6086
                     /locus_tag="IXG44_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAQ1657433.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWEIALIISLVLSIWMLIALWRRIR"
     gene            complement(6088..6720)
                     /locus_tag="IXG44_000695"
     CDS             complement(6088..6720)
                     /locus_tag="IXG44_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAQ1657434.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGNQSYLDAKN
                     MVLRESHIQKLTMQKTGLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTTIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            7040..7564
                     /gene="infC"
                     /locus_tag="IXG44_000696"
     CDS             7040..7564
                     /gene="infC"
                     /locus_tag="IXG44_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HAQ1657435.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            7636..7836
                     /gene="rpmI"
                     /locus_tag="IXG44_000697"
     CDS             7636..7836
                     /gene="rpmI"
                     /locus_tag="IXG44_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HAQ1657436.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            7889..8248
                     /gene="rplT"
                     /locus_tag="IXG44_000698"
     CDS             7889..8248
                     /gene="rplT"
                     /locus_tag="IXG44_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HAQ1657437.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            8600..9838
                     /locus_tag="IXG44_000699"
     CDS             8600..9838
                     /locus_tag="IXG44_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HAQ1657438.1"
                     /translation="MATVWMGLALVGNTFSPNSAMEGQGVIDVLTHIYTTGPAEYAKS
                     ILVNVFFGAFFGRVLVDSGIAATLIRKVVELGGDKPRITMSLLCIVTAVIFMSMTGIG
                     PVISIAVIVLPILMSLGISVPVALFSFMGSIMAGIFANIVNFKQYQTIYAGFNPAAES
                     YTYNDYFQIGMIGMIVSLVVVLTVANISMNKKKRYAMAANVPAEGGDAPMISWLAVLL
                     PVLGVVLLDLQIILGFILAGIWALLFTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGF
                     LMTLAMFNNSAAYASPYFSAIFGDWIPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSA
                     ILAVVLAVNPTMSPAFLFPLFAITTIAPQHLDITQSWVAWGLGYTKVTSREYMKKSIP
                     TGWIIGAILCLITFLLYGNA"
     gene            9886..12015
                     /locus_tag="IXG44_000700"
     CDS             9886..12015
                     /locus_tag="IXG44_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HAQ1657439.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLRENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKNLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKAIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPHTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            11990..12679
                     /locus_tag="IXG44_000701"
     CDS             11990..12679
                     /locus_tag="IXG44_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1657440.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEIYEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKEIE
                     KRCTEYDLDYK"
     gene            complement(13087..13773)
                     /locus_tag="IXG44_000702"
     CDS             complement(13087..13773)
                     /locus_tag="IXG44_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1657441.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(13909..14103)
                     /locus_tag="IXG44_000703"
     CDS             complement(13909..14103)
                     /locus_tag="IXG44_000703"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1657442.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(14153..14593)
                     /locus_tag="IXG44_000704"
     CDS             complement(14153..14593)
                     /locus_tag="IXG44_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HAQ1657443.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELEQLFQAYASLDDVNS"
     gene            complement(14597..15442)
                     /locus_tag="IXG44_000705"
     CDS             complement(14597..15442)
                     /locus_tag="IXG44_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HAQ1657444.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVDWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLEKQSKEQWLVIDGSALSLLVANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAVYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            15591..17009
                     /gene="pepV"
                     /locus_tag="IXG44_000706"
     CDS             15591..17009
                     /gene="pepV"
                     /locus_tag="IXG44_000706"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HAQ1657445.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(17085..18287)
                     /locus_tag="IXG44_000707"
     CDS             complement(17085..18287)
                     /locus_tag="IXG44_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1657446.1"
                     /translation="MKLFKKIFFAFVLLIASYCVLLTITFFIPQHSIESNAEKSLQMV
                     EKEGMYPSINQGNMLGTRLDNFTDHLMIRKTKADPELNSLENAMSMADYPRYWHGYQL
                     FLRPLLLGMSLGSIRMIYAAILFLLIGLTSYFLTKCSDIYLTTALLISLAIGNAATFF
                     FSMQFSNIWILTLLSVLLFLTKPQLSNTNQQLFLYFFVIGSLANFFDLLTTPVISWGI
                     PVIIIYYVTNKYLMDKRSSLSKQVGSFVFTGIFWGLGYGLTWVAKWILSSIILNKNIV
                     KDAINQILFRTEGNDKYPLHRLEMLKSNIRLMYPKVAILLLLITCLVFLYFAYRKRNS
                     WRPFQLLVLFVLAAVTPYIWYNILANHSQIHYWFTYRTQIITSFAILSAFAFLIPPEK
                     SKDELNYF"
     gene            complement(18274..20061)
                     /locus_tag="IXG44_000708"
     CDS             complement(18274..20061)
                     /locus_tag="IXG44_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1657447.1"
                     /translation="MHKKKENLFPTFFATLFASGFAFFTSYGLGTLNPTVIIGLAMVL
                     LLAFKQYGIELFSIKNKVAAIARLFFSAAFALTLSIGRQIQFQYVSANYTKTTIADGS
                     IFYFLVYFSIFVYSFLFLQSIYLSMPKLETFFTAQNTQAFSSRKYFLFFTVFLVLCWL
                     PYLLALYPGVVLPDSLSSVAQSMGDVPISNHHPVLFTLLVKLFVHDLPTDTINHGVFL
                     FSIFQTLITASAISYSLCWLLKRNIHFFPVLISLCYFAFAPVFPIYALNMQKDTLFSI
                     ACFLFSLAVFDFIFQREKPTILNQIYVIILALLTTYLRNNGIYVVTGTGLICGLIILK
                     QKRYQAFFQVVLGYTVIAGLLIHPLMARYAIPTETAEQLGVPLQQISRTIVMDGDLSK
                     KDKAFMNQLLPIKDYEAYSPSLADSIKWHKNFNNEFLNTHKKEFLSLWARNLPSNIKI
                     YTDAYILETFGFWVPGTKNSYGFLDTRVNTNNYGMKQVDLIEHLTGLSGMKKIIDARD
                     FFGSGTLLWLAIGSALLAIFKKKYRYLLLFLPGMLVFATIMIATPVAFSLRYVFVLAL
                     GLPVYILSPFLLDKREKMKTDIGDTHETI"
     gene            complement(20074..21027)
                     /locus_tag="IXG44_000709"
     CDS             complement(20074..21027)
                     /locus_tag="IXG44_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ1657448.1"
                     /translation="MESREKIAVLIPCYNEEATISTVIEDFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAAHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVKNTITYLYKTKIRDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMCKDYKPLLFFGIWTIFFFLFGLVMGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYQNKEMK"
     gene            complement(21212..21562)
                     /locus_tag="IXG44_000710"
     CDS             complement(21212..21562)
                     /locus_tag="IXG44_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ1657449.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKTSFLKEGAIEGAWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLDISRKKGAID"
     gene            21762..22841
                     /gene="pepA"
                     /locus_tag="IXG44_000711"
     CDS             21762..22841
                     /gene="pepA"
                     /locus_tag="IXG44_000711"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HAQ1657450.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            22984..23304
                     /locus_tag="IXG44_000712"
     CDS             22984..23304
                     /locus_tag="IXG44_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAQ1657451.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VIKG"
     gene            23326..23790
                     /locus_tag="IXG44_000713"
     CDS             23326..23790
                     /locus_tag="IXG44_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ1657452.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKALEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKQMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYAIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            23986..24591
                     /locus_tag="IXG44_000714"
     CDS             23986..24591
                     /locus_tag="IXG44_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HAQ1657453.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVASYGMICSARELHLPNAPEKKGILVLPEDAVVGEVFPK"
     gene            complement(24630..25919)
                     /locus_tag="IXG44_000715"
     CDS             complement(24630..25919)
                     /locus_tag="IXG44_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAQ1657454.1"
                     /translation="MDRKNVTPKMKKNKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVMMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            26347..26826
                     /gene="rlmH"
                     /locus_tag="IXG44_000716"
     CDS             26347..26826
                     /gene="rlmH"
                     /locus_tag="IXG44_000716"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HAQ1657455.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYGLAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            27179..27493
                     /locus_tag="IXG44_000717"
     CDS             27179..27493
                     /locus_tag="IXG44_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ1657456.1"
                     /translation="MELKFVIPNMEKTFGNLEFAGEDKVVQRRINGRLTVLSRSYNLY
                     SDVQRADDIVVVLPAEAGEKHFGFEERVKLVNPRITAEGYKIGTRGFTNYLLHADDMI
                     KE"
     gene            27509..27895
                     /locus_tag="IXG44_000718"
     CDS             27509..27895
                     /locus_tag="IXG44_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ1657457.1"
                     /translation="MMRLANGIVLDKDTTFGELKFSALRREVRIQNEDGSVSDEIKER
                     TYDLKSKGQGRMIQVSIPASVPLKEFDYNARVELINPIADTVATATYQGADVDWYIKA
                     DDIVLTKDSSSFKAQPQAKKEPTQDK"
     gene            27924..29309
                     /locus_tag="IXG44_000719"
     CDS             27924..29309
                     /locus_tag="IXG44_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAQ1657458.1"
                     /translation="MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQIN
                     WQDFNLSQADKIDIPYLIISFSVAILICLLVAFVFKRVRYDTVKQLYHRQKLAKMILE
                     NKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKNGLIQIRVEITLGKYQDQLLH
                     LEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRLMKNVWWEY
                     DKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRK
                     EDLLSCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTK
                     ENTAVMNKLKQIVMLGRQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYG
                     MMFGSDVQKDFFLKRIKGRGYVDVGTSVISEFYTPLVPKGYDFLEEIKKLSNSRQSTQ
                     ATCEAEVAGVD"
     gene            29312..29465
                     /locus_tag="IXG44_000720"
                     /pseudo
     CDS             29312..29465
                     /locus_tag="IXG44_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            29488..30693
                     /locus_tag="IXG44_000721"
     CDS             29488..30693
                     /locus_tag="IXG44_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ1657459.1"
                     /translation="MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIE
                     TGKVKPSEDLQQSLWEALERFNPDAPLEMLFDYVRIRFPTTDVQQVVENILQLKLSYF
                     LHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGRGCRQFESYLLAQQRSWYEFF
                     MDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRSGELVRKEE
                     KECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAV
                     RDLLVYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKP
                     EPYSFQRTLNWLSHQVAPTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVK
                     EQDVITTKK"
BASE COUNT         9741 a         5771 c         6256 g         8987 t
ORIGIN      
        1 tgcttccgcc gtttatacgt gtttagagcg tgggataaaa gtgttcttta cttttgtccc
       61 acgctctttt ctgtgcgtct tccatacttc actaagtcga tccctcttgc ttcgcaccaa
      121 tccttcactg gtccggaaaa gatcagtgat tgttggtgta gggccgcggt attcgttgcg
      181 ccgaccatag tataaagcag acgaagctct tcttgccatt gcttcatcag tataatagtt
      241 tcttctacgc cgtgattcat cagatgcgtc agtacggttc ctgaagtacc cactgcattt
      301 gcgccgagac aaagggcttt aaatatgtca taggcattgc ggatacctcc ggaggctagg
      361 atttccatag aagtgtctgc ttcatttgct tcaagcaatg aagagactgt cgattgcccc
      421 cagtcagcta aataactcag ttcccgcttg gagcgacggg cgttttcgat ctgggtgaag
      481 cttgttccgc ttcgaccgct gatatcaact gtatgaactc ctaaagcagc tagatcattg
      541 agcgtttctc ttgtcatgcc aaaaccgact tcttttacga tgagggggac gtcaatggct
      601 gtttgagtct cttgaataag tactttccaa ttggtaaaat cacggtctcc ctcaggcata
      661 accaactctt gaggtgcatt caaatgaatc tgtaaggcat ctgcgtgaaa caatcggata
      721 gcttcttggg ctctttcgac agaagtccct gcaccgatgt tggcaatgat tttcccgtga
      781 ggatattctt gtcgcataat cgtataagta tcggctaaag aagggtcttt caaggcagcg
      841 ctgactgaac cagtggcaat catcaaatct gcttctcttg cgacgattgc aagatcttgg
      901 ttgatttttt ttgttttttc gcttccgcca gtcatcgcat tgatataaaa tgggctgctt
      961 agtgtaaacc caactgcctg tgtacttaga tcgacatcag aaactgctat ttgcggcaaa
     1021 gggttatgga tgatccgtac gaagtcaaat tcgttttttt gtttatcatg aaaggcttta
     1081 gctagtgaaa catgttcgtc ttttcgattc atcggttttc ctttctttga tcgctataga
     1141 catgtaacgg cagtggagtg atttctgctt tttcccatgc actcattaaa ggaaggatgc
     1201 cagatttctg gtcaacaatt acgattccgc aatcgccacc acctgcacca gaagattttg
     1261 cggctccttc atactgttca gctaaattac acaatttgtt caaagcaggc gtttcgatga
     1321 cgaccccagt gattgcagat aaatcatgca gtaattgtcg gttttttcga atcatctgtt
     1381 ggatcaacgt tacattattt tctttaaacc ctttgatcat ttcgttgaca cattctgtac
     1441 tgttttttaa gaaaagctga taagccacca ttttgtcttc tctcgaacgg tgaacttgat
     1501 cgaccaaatc agaagtagag gcagggctac ccgtccaacc aatcaataaa cgtaaatctt
     1561 caggagcaat caatggctca atggatagac ctggccaatc cagggcaagt aactcactga
     1621 tagaatgctg agtttcttgt tcttggagcc aaggatggtc gaaggttgag aaagcgatcc
     1681 agccaccata acagctagca gcgatgtcgc cgcaggaacc attatcttga actgctaaat
     1741 tggctagtgc tgcaatcttg aaaatctcca attgagataa attcaacgcg taaaaaacat
     1801 tcaaggcttt gaccgttgca acggtcacgg caccgctaga acccaatcca tatttccggc
     1861 catttgaact gtctaactca cttgttactt tcagatcata aaaggataaa agaatgtttt
     1921 tttcttgtgc atacttttca gtcaaacgaa tagcagcaag gatataatga aaaggatttt
     1981 cccgaatatc taaaactaat tctccgttgc gtcttgtcca acgtacaggc atcccactat
     2041 attgtgcaga ttggatactt ccgacttttc gtgcggattc tacagtgact gttacgaatt
     2101 gatcgactgc agcgataact gctggatgtc ctgtttcaac aactgcatat tctccggcaa
     2161 tatagagttt gcctggtgca gatacttcaa tcataaaaat ctccctttat tcaataatcg
     2221 caattcctgg accagcatgg gcagtaatta cttgttcttt gctaaataat ttggtaaatg
     2281 tttcttgtac ctcagataaa tggctgtttt ccactaaaac tttgacatta gggccagcat
     2341 ccatcgtaaa gtagcatgga atgccttttt ttctcaattg ccgcaccgca tccatcgctt
     2401 ttaagctgtc aggagaccag taagtgaatg gaggctgggc agctaaagtc gttccatgca
     2461 tttttagtcc gtttctttcc atcgtttctc cgagaagttg gaaatctttt gttttgattg
     2521 cttgtttcaa ttgatataga tccccttcaa cggaatctaa ccagccttga taaaaactgg
     2581 atgtttcgac tgttcgacgc atcccatttc tgctggacac ttcttttttc tggtcgttga
     2641 tcaaaacgaa aaccattgct aaatcgtctt cgaaagaatc ggaagggact ggcttagcgt
     2701 aagaacttaa gtcgtcatgg cctttttccc attcaacgaa accaccgaaa atacttcggc
     2761 aagctgaacc agacccgcgt cgagcaaatc ttgaaagaga gagatcgtct aatccaagct
     2821 taagagcagt attgcaagct cctgctaaag cagctagccc gctggcagag gaggctaatc
     2881 cagctgcggt aggaacgaaa ttctgactga ttactgaagc ttttagtgag aggccggctt
     2941 ctttacgaaa aaggtcaaga aactgactga ctttttttgt tgctttttcg tcttgtaagg
     3001 tgccatctag ataaaattca tctaccatat agctatcaga aaagatgact tctgtctctg
     3061 tataaaatgc atccaatgtt aacgaaaggc tgttgttcat tggaaggatg agttcttcat
     3121 ttttcttacc ccaatatttg attagagcaa tgttggtata tgcgcgtgct ttgcctttaa
     3181 acataggtat gtactcctaa tccttgtatc caagtttcag cagcacccgc atcttcaagt
     3241 gcttgactga tttcttgggc ttttgttttt gtttgtgcta atgcaatcat acagccaccg
     3301 cgtccaccgc cagttaattt ggcacccaat gccccggtat cttttgctgt ttggatcaat
     3361 agattaagaa aatcattgct gattgtcagc ttttccagaa gtgactgtga ttcatccatt
     3421 gtctgtgcca acgtttctgg tgaattctca atgattgctt gttttgcttg cttcgtcaag
     3481 tatcctaatt tttggatcat ttgtccagtt tcatgaggct gggtttcaaa gaggtgagcg
     3541 acatctttga cggcttctct tgtttgtcct ttgattcctg tatcagcgac aatcaaaaaa
     3601 gcatcgatgt tcaaagaaaa gtagtcaaaa ggatgccctt ttgtaaaata aatcggctgc
     3661 aagctgctag tagcggctgc atcgattcca ctaggattac cgtgggcgat tttttccgaa
     3721 agctggacat tttctaatag tatttcacga gacaaaggaa atgctaagta atcataaaaa
     3781 gcacgagtga ctgctgtggc gactgcagcg cttgatccca ttccacgttc agcaggaatc
     3841 gtgctttcaa tggtcagttg caagtttttt tggatgtcat gctgtttttt taagtgaatg
     3901 aataattttt tgatattttt taatgcatga ggagcttcgg ctaaatttcc tgaatagtag
     3961 gaagaaacca gttgatcgtc cattgcatcc agctcttcaa ggaatgcggt gacttttgtt
     4021 gcatagaaag gaaaggcaat cgctggttct ccataaacaa ccgcgtgctc gcccatcaat
     4081 atgatctttc cgcttgattc tccttggcca tagtttgcca taatctaact ccttctcacg
     4141 taaaagtgat ttttaagcaa aaaataaagc gcccaaaacg agttgcgctg cttcacgaat
     4201 ttttttgtcc actaagggtc tcaaaaaaca ttcctccttt attttactaa aaccatctca
     4261 gaagtaaaac taagaatacg ttttctaaag aaaaaacact tttttcttaa gaattttttg
     4321 actttttttc ttctttgact agaaaaaata caattgacac tactagatat tgggggtata
     4381 tttaaaaaag gttggagtta aacacaatat ttagtgatga agtgagctgg aggtaaatga
     4441 gcgtgatcgt tttagcaggt acaattggtg ccggaaaatc aagtttaaca gaaatgatgg
     4501 ctgaacactt tgatagtcag gcattttatg aatcgattga tgataatgaa gtcttgccct
     4561 tattttatgc aaaccctgaa cagtatgctt ttttgctgca aatttatttt ttgaataaac
     4621 gttttgcaag catcaaacaa gcaatgaaag atgacaataa tgtattggac cgttcgattt
     4681 atgaagattc actgcttttc catctaaatg cagatctagg aagagcaaca gaaacagaag
     4741 ttcgtgtgta tgatgaactg ctggaaaaca tgatggaaga acttccctat gctgcccata
     4801 aaaaacatcc ggatttatta gttcatattc gtgtatcttt tgatacgatg ttagagcgta
     4861 tcgaaaaacg tggtcgttcg tatgaacaat tgtcctttga cccaagtcta tacgattatt
     4921 acaaagaatt gaacagacgt tatgatcaat ggtatgaaga atacaaagaa agtccaaaaa
     4981 tccaaatcga tggagaccgc tataattttg tggaagatcc tcaagcaaaa gaagaagtct
     5041 tgaaaatgat cgaagaaaaa ttagcagaga ttcgccaaac caaagtatcc atttgattta
     5101 cagttgctca aataaagggt aaacctttat ttgagcgact tttttcttta tactggaaac
     5161 aaatgtcagg aggatagagt atgcgggaaa aacactattt taatcaagac cgtaattttt
     5221 ggatcaatgt acagcaaaac gatgtgctag ctgttaaaaa cttaaaagaa gaatatggca
     5281 ttagtgacga gatgctgact tattctctag ataaaaatga acgggcgcgc gtagagtacg
     5341 attcttttga tgaagcgtta ctgcttgtat caaatgttcc gcaccagcaa aaagtcgata
     5401 atcattatga aactagtcct atcgctttta ttttaaaaga agacggtttg tttacattta
     5461 ccacgccgaa taccgagtac gtgattcgac taattcgatc gcttttggaa cggatgccag
     5521 acatgagtgt atacagttta ttgtttcgta cattgttttt gatttctgat tccttttttc
     5581 ctttaatcga ggaagtgaat agtgaacgtc agcggcttaa tctgaaatta agagaaaaaa
     5641 caacgaacaa gaatctgctg caactttcag atttagagat cggtttggtt tacttagtta
     5701 caggaacaaa acaaaatgta gtacttttag aacaaatcaa ggcactggct atttatcgca
     5761 agttgtcaga aaaggaaaaa gaagagttgg acgatgcact gatcgaagca cgacaagcag
     5821 ttgaaatgac gaacttagct tcgcagattt tagaccagct ctctggcact tataataatt
     5881 tgctgaataa caacttgaat gacacgatga aatttttgac ggtctggtcg ctgatcttga
     5941 ccgttccaac tattgtgaca ggattcttcg gaatgaatct ccagttaccc tttacccatt
     6001 ccgttttcgg atgggaaatc gccttgatca tcagtttggt cttatctatt tggatgctta
     6061 ttgcactgtg gcggcggatt cgttgatcta gtctttgatc tgcaaaccaa gggcttgggc
     6121 gatcacaagt gcttgctctt gattgacgat tagtcccgcc aaagaatctt gagataaagc
     6181 gattgttgtg aagaaattag tcgtaaagtc taatcccttc agagaagttc gaaaccagtt
     6241 cgaaccggtt aactgattgt cagacaaaaa aaggtttttc caagtgacct cataaaattc
     6301 gctgtcttct aattgacaat gatcgaagga gacagctttc aagtttgtac tactaaagga
     6361 agccatcgaa gcaatacaat cagtgaaagt acagtctcga agataacttt cggcaaaatt
     6421 cgtacctgtt aatttacatt gatgaaacac gacttggtgg aaactgccac caatccactc
     6481 cagattggaa aaatcacatt tttcaaaaat gacattactg cattcaaaat gacgtaaacc
     6541 tgttttttgc atcgtaagtt tttggatatg actctctctt aataccatat ttttggcatc
     6601 gagatagctt tggttacctc cgttgatcag aagaccagag aagatctcct catcttctaa
     6661 ataaaactct ccctttgtaa gaagaggaag agaaggtgga ataggttcga tttttttcat
     6721 gtgggttcac ctcgtttttc tttagtatag caaagaagcg ggaatcactg ctagaaagcc
     6781 tgctaatcta atcattttca gaaaaggaag acaaactgta tgaaaaacat tgacaaaaag
     6841 attagttttt gctatactat aatggttgag aaataaaagc agaagcaccc gcttctcgcc
     6901 ttagttgata atgtcactgg gctagatagt tttcactggt tctattaggt gaagtacgcg
     6961 cgggttctgt aagagaactc gtttttttat tgccgtttat tcggtaagct tttctttctc
     7021 tcataatatt tggaggtgaa tgaccatagc aaaggatatg atggttaacg acggcattcg
     7081 tgcacgagag ttacgattga tcggttcaga tggtgagcaa ttaggagtta agacaaaagc
     7141 agaagcattg aatattgcag aacaagcaaa cttggatctt gtgttagttg cccctggtgc
     7201 gaaaccacca gttgcgcgaa tcatggacta cggtaaatac cgtttcgaac aacaaaagaa
     7261 agaccgcgaa gcgcgtaaaa aacaaaaagt gatcaatgta aaagaagttc gcttaagtcc
     7321 tacgatcgac ttgaatgact tcaatacaaa acttcgtaat gcacgtaagt tcttggaaaa
     7381 gggcgataaa gtgaaagctt ctatccgttt caaaggccgt gccattaccc ataaagaaat
     7441 tggtcaaaaa gtcttgaatc gcttagctga agaaactgcg gatattgcaa cagtggaaca
     7501 aaaagcgaaa atggacggac gcagcatgtt cttgacgctg gcaccgaaaa acgacagtaa
     7561 gtaatttgaa tgactaactg gctgttggac agccgatagt cggataacga aagattttga
     7621 ggaggaaata tagtcatgcc aaaacaaaaa acacaccgcg gatcagcaaa acgtttcaaa
     7681 cgtactggta aaggcggatt gaaacgcttc cgcgcgttta caagtcaccg tttccacggc
     7741 aaaacaaaaa aacaacgccg tcaattgcgt aaagcaagca tggtttctag tggcgacttc
     7801 aaacgtattc gccaacaact agcaaaaatg aaataagaag tacaaataac agaatctgaa
     7861 gatttatttt tattaggagg aattatacat ggcacgtgtt aaaggtggaa cagtaactcg
     7921 taaacgtcgt aaaagagtgc taaaattagc aaaaggttac tacggttcaa aacacacttt
     7981 atttaaaaca gcaaaagaac aagtaatgaa ttcatacaac tacgcatatc gcgatcgtcg
     8041 tcaaaagaaa cgcgacttcc gtaaattgtg gattgctcgt atcaacgcag cagctcgtat
     8101 gaatggcttg agctactcaa aattaatgca cggtttgaaa gttgctgaaa tcgacatcaa
     8161 ccgtaaaatg ttagctgact tggctgttaa cgatgcagca gcatttactg cattggctga
     8221 ccaagcaaaa gaagctttgg ctaaataagc taaacaatta taaagaggaa agaagataac
     8281 gaaaagttat gttctttcct ctttttttat ttacaatcct ccatcaaatg aaacaggaca
     8341 ataacgtata agtctaaaat aacataatta aggtttttta attgaaaact gaacatttat
     8401 ggtcatcctt taatgaaagc gctaactaca taagttagaa tgagtttgac tgatatatta
     8461 gtttgcttat cagaaaaaca aaatggacgg aggaataaca tgagtgatat tattattggt
     8521 attttgttag ttatctcatt ctttttcatg gtctggtact gtgtaaaagg ctacaacttg
     8581 atggtcggct tttgtcatca tggctactgt atggatggga ttagcgctag tcggaaacac
     8641 gttttcacca aactcggcga tggaagggca aggagtaatt gatgtgttga cgcatatcta
     8701 tacgacgggg ccagctgagt acgccaaatc gatcttggta aatgtcttct ttggtgcctt
     8761 tttcgggcgt gttctagtag atagcggaat cgcagctacc ctgattcgaa aggtagttga
     8821 acttggaggg gacaagccaa gaatcacgat gagcttacta tgtattgtaa ccgctgttat
     8881 attcatgtcg atgacaggaa tcggaccagt gatttctatt gcagttattg tgttgccgat
     8941 cttgatgtca ttaggtattt cagtaccggt tgcactgttc tcatttatgg gctcgattat
     9001 ggctggaatc tttgctaata tcgtcaattt caaacaatat caaacgattt atgctggttt
     9061 caatccagcg gctgaaagct atacttataa tgactatttc caaattggta tgattgggat
     9121 gattgtaagc ttagttgtgg tactaacggt cgctaatata tcaatgaaca aaaagaaacg
     9181 ctatgcaatg gcagcaaacg taccagctga aggtggcgac gcaccaatga tctcatggtt
     9241 agccgtatta ttaccagttt taggggtcgt tcttttagat cttcaaatca tcttaggttt
     9301 tattctagca ggtatctggg cattgctgtt tactggaaaa ttgcgcggag gttataaaga
     9361 aatctgtcgt cagtttgcga aattatttac agatggcgca gtcgatgttg caccaatggt
     9421 tggtttcttg atgaccttag cgatgttcaa taattcagct gcgtatgctt ctccttattt
     9481 ttcagctatt tttggagatt ggataccgca atcaccatta gtcttggcaa tcgtatttgc
     9541 tatattgaca ccactaggat ttttccgcgg tccaatgaat ctagtcggtt caggctcagc
     9601 tattttagct gtcgtgttag ctgttaatcc aacgatgtcg ccagcctttt tgttcccgtt
     9661 atttgcaatt acaacgattg ctcctcagca tttagatatt acacaatctt gggttgcttg
     9721 gggattaggt tatacaaaag taacttctcg agaatatatg aagaaatcca tcccaactgg
     9781 ttggatcatc ggtgcgatct tatgcctgat cacgttctta ttgtacggga acgcctagta
     9841 aacaaaacgt cgataaaaac gattagataa atggaggaaa agaaaatgaa acgttttgtg
     9901 cgaatgggaa ttgatgttgg cggtacacat acgaaagcag tcgcaatcga taatgcgacg
     9961 cacgaaatca tcggaaaatc ttcagtgaaa acaacccacg atgatgtccg tggagtagcc
    10021 gcaggtgtcg ttcagtcttt tcaaaattgt ttgcgggaaa ataatatcag tccagaagat
    10081 gttgtgtttg ttgcacattc taccacacaa gcgactaatg cactgattga aggagatgta
    10141 gcaaaagtcg gcgtaatcgg gatggcaaaa ggtggattag aaggtttttt agcaaaacga
    10201 caaactagat tgaacgatat cgatttaggg aataagaaaa aaatagagat tgtcaatgca
    10261 ttcttacctg tcaaaaactt aaatgtagac cgtgtttcag aaacgatttc ttctttagaa
    10321 agagaaagag cggaggtctt ggtttcttct atggcatttg gagtagataa tggggaacca
    10381 gaacgagttg tttatgaagc agcatctgtg aaagcgatcc ctacaacgat ggcttctgac
    10441 atcacaaaat tatatggatt gacaagaaga actcggacag cagcaatcaa cgcatctatt
    10501 ttaccaaaaa tgctagatac ggcaacctcg acagaagact ccgtacgaga agcaggggtc
    10561 aatgtttctt tgatgattat gcgcggagac ggcggagtaa tggaaatcaa tgaaatgaaa
    10621 aaacgacctg ttttaaccat gctttccggt cctgctgcat cagtcatggg ctctttgatg
    10681 tacttgagag cgtctaatgg tgtttatttt gaagtaggcg gaacaacaac caatatcggg
    10741 gtcattaaaa atggacgtcc agcaatcgat tattcaattg taggtggcca cccaacatat
    10801 atctcttcat tagatgttcg ggttctaggt gtggcaggcg gttcgatggt acgggctaat
    10861 cagtcaggaa tcatcgatgt cggtccacgt tctgcacata ttgccggtct agattatgca
    10921 gtatttacgg agacagaaaa aatcaaaggt cctaaggttg aatttttctc tcctaaagaa
    10981 ggagaccctg ctgattacgt caaggtagtg atggaagacg gagaagaagt gactattaca
    11041 aatacatgtg cagccaatgt tcttggatta gtgcaagaag aacatttttc ttatggaaat
    11101 gtcccatctg cacgaaaagc catacaggca ttagcagact attgtcatac gacggttgaa
    11161 gacatcgcag agcagatcat ggaaaaatcc tatgctaaga ttgaaccggt gattttagaa
    11221 ttggcagaca agtatcactt ggagaaagat cagatttctc ttgtaggtgt agggggcggc
    11281 gctgcctcat tgattactta ctttagtaat aaaatgggtg tgaaatattc gattcctgaa
    11341 aacgccgaag tgatttcttc tattggtgta gcacttgcaa tggtccgaga tgttgtagaa
    11401 cgtatcatcc cttcgccaag taaagaagat atacgatcgt tgaaaaatga agcgatgaat
    11461 aaagcgattg aatcaggcgc tacaccagaa tcgatcgaag tccacgtaga aatcgatcca
    11521 catacttcaa aagtaacggc tatcgctact ggttctacgg aagtgaaagc aaccgatcta
    11581 accaaggaga tcacaacaga agaagcacta gagcttgctg cagaggatat gcgattaaat
    11641 aaaaatgaag tttgcttatt ggaaaataca cctttctttt atgtatgcgg cgaacagaat
    11701 cgttcaaaaa atgctggaag tcttcggatc atcgatcaaa aaggatttat caaggtccaa
    11761 cgtggacatg cttcatgtat gaaaacaact gcagccaatt atatgacagc tgtcgaacaa
    11821 ctttgggaag atatggcagt ttatcaaact gaattgattg cccgaccaga attttatctc
    11881 tgcttaggag caagagtctc tgattttaca gcgacggatc tggaacaact acagcttttg
    11941 atggatctgg aagtatctac tatggaaccg gaggaagaag tgatcgtcgt tgccggaaat
    12001 atcaaacaaa cataagttag aagagttact aaacgaatta caacagatcc ccgaagaaat
    12061 ttggggattc taccagtttc ggcgagattt attttggaaa aagattcctt tgtccaagca
    12121 gaaaattctt atacaacaat caatcgattg tggtatcgaa acggcatgta gcatcaaaaa
    12181 aaagtaccct tttgccgatg taggagaaat ctatgaacaa atggctgttc caatcgtttc
    12241 ctgtgaatca gagcaaataa acgaacgaat cacttttgca acttatgcag aagatgaagg
    12301 gatccgattg atgacggagc cgttagaaaa attaaaatgt tcaggactag cttcgatctc
    12361 taaagaaaca gcgcaagctt tgattattgg acatgaacta tttcaccaca tcgaagcttc
    12421 tgttaaggga atttatacgc aaagcgaaaa aattgtgctg tggcggttgc ctttttatac
    12481 acatcaatca aacattcgtg cattgagcga gatagcagcg atgagttttt caaaagagat
    12541 gaatcaaagc gatttttcgc cttatgtatt agaggcagtc ttactttggc catataatga
    12601 aattcgtagc caagggatct tagaagaaat aaaagaaata gaaaaaagat gtacggagta
    12661 tgatttagat tacaaataaa gagactgtga caaaagtcgt gtcacagtcc tttttttccg
    12721 aataaacggt gttctcctgt caactcctcg gtcacaagtc aaaatattca acaaacatga
    12781 gaagctgttt tttgaaattg ttccttgtga ctcggagatg aacggcaggc tcacgacctc
    12841 ttggaaaaag gtgttcaaaa gcttgcatct gcggtaataa gaacagccaa gttaaaaaca
    12901 taagaagcta tttttacttg gctgttctta ttacttgatg cagggcagct ttttcacaac
    12961 ctctcaataa acggtgtgca atcagctgac cctcggtatt agctcaaaaa aaacggcaaa
    13021 atatgaggaa ctattttgcc gtttatttct ctaatactca ggtcaaaacg ctgattccaa
    13081 aacctcttat ttgatgggtc tgtcctgaat atcttcaaaa taatcggggt aattatgaat
    13141 cacgtattct tgctcctcga taatcatatg taaatgcatc gtgcaaagaa atccgacttc
    13201 atcaaagtta tgatcaatca ttgattgaag cattgtctga tgttcatcta atactcgacc
    13261 ccagtctaat tctgaaatcg tcaaacgtaa ccatctgaaa cggttcaagt gcgtactata
    13321 actttctagc caatcccaaa tttctcgttt tccagcaatt tcataacaag ttcggtgaaa
    13381 atcagtatcc aaatcaaaaa aagctttttt attttgagcg atcactgcct gatcggtttg
    13441 ttctagtatt ttttctagtt tagcttgatc ttgcttggtc attttcgcac tgcattcttg
    13501 caggatcggt ttttccaact tttcccgcgt ataacgggct aatgaagcag atcggatatc
    13561 gatttttgaa acataagtgc cggactgtgg aatggttttt acaagctctt gattgcgcag
    13621 ttggatcagt gcttctcgaa tcggtgtacg cccgatagct agttgttctt ctaacacttt
    13681 atcggagatt ttacttccag gtgctaactc ggaataaatg atttgtttac gtatagattc
    13741 ataagcttgc tgttgcaaat tattgataac catttaacct tcctttctat gcagatataa
    13801 tctattataa cataaatcgc ttacaaatcg tgtgggcata aaaacacagt ctgcaaaaaa
    13861 agccctgata ggcatgccta ttagagcctc gaaaagaaaa atattgaatc aatctggtcg
    13921 tacctcttgg atcggtttga cacgaccgcc gattctttta gcaaaagctt cggattcttt
    13981 tttgtctttg aaaatgagca gattgtctaa gtttttttcc cgagtgcgac agccagtttt
    14041 atcgatgtaa ccgtgcatta ctttaacgac atacatgggg atcacctgct tttttctttt
    14101 cattatacga actactcgac acaatacaag aaaaatgctt agacggtatt tattaagaat
    14161 ttacatcatc caaagaagcg tacgcttgga ataactgttc taactccgga ttgactttgc
    14221 tcagcttcac taatcgttgc gaatcagaaa gtaaaaaaca gtgtttacta ctgccagtag
    14281 tttttaattt tttgtcttca gttcccacaa tttcgtagct aaaatccaac cgtgttcccg
    14341 tatacttttg gatagttggg tggatgctca ctttttctcc gtaatggatc atttctttat
    14401 agctgcaatt tacttccaga acggggatga tgatcccaga ttcttcgatt tttttatagg
    14461 gaaaccctag atgtaccaat aaggctaccc gagcctcttc aaaccagcgg atataattgc
    14521 tgtgatgaat aatccccatt tgatctgttt cataataaaa aggttctcgc acataacctg
    14581 taaatttctc catgactcat gcctcgtttt ccattgattt tttacaaaaa taattcatcc
    14641 acttcggaag tgcttcgctg atttgtgtcg gcaaaacaat atattgttct tttgccagct
    14701 ggtctccaat gaaactatga agataaacag ctgctgcaat cgtttggtca ttttgaggaa
    14761 actgggctaa aaatcctgtg atcatcccag ctaaagtgtc acccatgccg cctgttgcca
    14821 tagcagcaga acctaaagga ttggtgtaga agtgctggtg accataaatt tttgtctcat
    14881 gacttttcaa aacgagcaca gcaccgattt cctgttgctt ctttttgttt tctttatccc
    14941 gttgttcttg tatcggtaga gaactcaacc gttgccactc catttgatga ggtgtaagta
    15001 cagtttgttc gggatagggt agcaaaagat gattagcaac aagtaaactt aaagcagagc
    15061 catcaatcac cagccattgt tcttttgatt gtttttctag tacttgtttg aaacgttcca
    15121 aactcttaga agaagtaccc aatccgggac caatcaagag tacatctgct tgctttatta
    15181 cggaaagaaa ctggtcagta tccgtccaat ctacaatcat tgcttctggc agatgactgt
    15241 gaagagcact ccgatttttt tcatcagtta ctacagtcgt gagacccgct ccactgtgta
    15301 cggcagctaa agcactcata atgattgctc cgccatattg ttcattgccg ccaatcaaaa
    15361 cgatacgtcc aaatgttcct ttatgtgaaa tctttggacg aggtgtgatg actcttttca
    15421 ctagttgttc gtttatcagc atcgaaaaac ctccttttac tcattatagc gtatattttt
    15481 tttagagaaa aacagataag aaaaggaatt gtcagatttt ttgaactatt tttttctgta
    15541 atttgtggta tcatgaacac gaagacttaa ataagtggag gattaacttt atgacaattg
    15601 attggcaaaa agaagtagaa gcacgtaaag aagacttgct tgaagacttg aaaaatcttt
    15661 tacgtgtcaa cagcgagcgc gatgattcaa aagtaacacc agatgcacca tttggacctg
    15721 gacctcgcga cgcattgaaa catatgttag cttatggcga acgtgatgga tttaaagtaa
    15781 aaaatgtcga taactatgca gggcatattg atcttggcga aggagacgaa acgctaggga
    15841 tatttggaca catggatgtc gttcctgcag gagatggctg ggacacagat ccttatgaac
    15901 cagtaatcaa ggatggaaag atctttgcac gcggatcaag cgatgacaaa ggtccaagta
    15961 tggcagctta ctatgcgatg aagatcatta aagaacttga cttgaaacta tcaaaaaaag
    16021 ttcgcttcgt agtcggcagc gatgaagaaa gcggctgggg cgatatggct tattatttcg
    16081 aacatgaaga agaaccagat tttggtttct caccagatgc tgaattccca atcatcaatg
    16141 gtgaaaaagg aaatgtttct ttagctttac gcttcaaagg cgacaatgca ggagattatg
    16201 tcttgaaatc ttttgtttcc ggacttcgcg aaaatatggt gccaggaaca gcaactgcag
    16261 cacttcaagt accaagtgca gatgcagcta tcgcgatgga agaagcattt taccaattca
    16321 tcgaagcaaa tccagtaagc ggaacgattg aagcggacaa tacgtatgta aaaatcgaat
    16381 tagttggtaa aggcgcacat ggtgcaagcc cacaatctgg tatcaatgca ggttctttct
    16441 tggcattgtt cttagataac tatgaattcc taggatctgc taaacaattt atccatgtag
    16501 cagctgctta tgtccatgaa gatttttatg gagaaaaact aggagtcgct tatgaagatg
    16561 aaaaaatggg caaattgaca atgaatgctg gactatttgc ttttgaagaa aatggtacag
    16621 aggaagcgaa cttcatcaat atgaatttcc gttttccaaa aggtgtaaca gtagacggat
    16681 tgcaaagtga catcgaacaa acagtggggc aagaaggcgc aacagttact cgaggtgctc
    16741 gcgtaatgga accacattac gtaccaatgg atgatccatt ggtggctaca ttattgcaag
    16801 tctatgaaga ccacactgga gaaaaaggct acgaacaaat catcggtggc ggaacttacg
    16861 gacgtttgtt gaaacgtggc gttgcttatg gagcgatgtt cccaggatat actgatacaa
    16921 tgcatcaagc aaacgaattt atgagtcttg atgacttgtt cagagcaaca gcaatctatg
    16981 cagatgccat ctatcgtttg gcgaaataaa aatactaaaa aactccggct atctctgaca
    17041 gctggagttt ttatgtttaa aaatgaatta gtacatcggt attcttaaaa gtaatttagt
    17101 tcgtcttttg atttctcagg agggatcaaa aaggcgaaag cactaagtat agcaaaactt
    17161 gtgatgattt gcgtacgata cgtaaaccaa taatgaatct gtgagtgatt agctaaaata
    17221 ttgtaccaaa tatacggcgt tacagcagct aaaacaaaca aaactagaag ttggaacgga
    17281 cgccagctat ttcgtttacg ataagcaaaa tagagaaaaa caagacaagt aatcaatagc
    17341 aataatattg ctacttttgg atacatcaat ctaatattgc ttttcagcat ctctaatcga
    17401 tgcaacgggt atttgtcgtt tccttccgtt cgaaagagga tttggttgat tgcatctttt
    17461 acaatgtttt tgttcaatat gatcgatgat aggatccatt tagccaccca tgtcaaacca
    17521 tagcctaatc cccaaaagat accggtaaaa acaaaactgc ctacttgttt gctcaatgaa
    17581 ctgcgtttat ccattaaata cttatttgtt acataataaa taatgatcac aggtatcccc
    17641 caagagatga ccggtgttgt caacaagtca aaaaagttag ctaaagaacc aatgacaaag
    17701 aaatacaaga ataattgttg gttggtgttg gagagttgtg gttttgttaa aaatagtaat
    17761 acagacaata aagtcaaaat ccaaatattt gaaaattgca tactgaagaa aaaggttgca
    17821 gcatttccta tagcaagcga aatcaacaag gctgtcgtta aatagatgtc gctacacttg
    17881 gttaaaaagt agctcgtcaa tccaatcagc agaaaaagaa tggctgcgta aatcattcga
    17941 atactcccca aactcatccc tagcagtaaa ggtcgtaaaa aaagctgata accatgccaa
    18001 taacggggat aatccgccat agacatcgcg ttctccaagg agttcaattc aggatctgct
    18061 tttgtttttc gtatcatcaa atgatcagta aaattatcta accgagtgcc tagcatattt
    18121 ccttgattga tagaaggata cattccttct ttttcaacca tctgtagaga tttttctgca
    18181 ttcgattcaa tagagtgctg cggaatgaaa aaagtaattg tcagtagtac gcaatagcta
    18241 gcgatcaaga gaacaaaagc aaaaaatatc tttttaaata gtttcatgag tatctcctat
    18301 atctgttttc attttttctc ttttatctag taaaaaagga gagaggatat aaacaggtag
    18361 acctaatgct aaaacaaaca cataccgtaa actaaaagcc acaggagtag caatcataat
    18421 agtagcaaaa acaagcatac cgggaagaaa aaggagtaaa taccgatatt ttttcttaaa
    18481 gattgctaaa agtgcactgc cgatagctaa ccacagtaat gtgccggaac caaagaaatc
    18541 acgagcatcg atgatttttt tcattcctga caatccagta agatgttcaa tcaaatctac
    18601 ttgcttcata ccataattat tcgtgtttac acgagtatct aaaaagccgt aactattttt
    18661 tgtaccaggt acccagaacc cgaacgtttc taaaatataa gcatctgtat agatttttat
    18721 attacttggt aaatttcttg cccatagtga tagaaattct tttttatgtg tattcaaaaa
    18781 ctcattgtta aaatttttat gccacttgat tgagtctgca agactaggag aatacgcttc
    18841 ataatctttt atcgggagta gctgattcat gaatgcttta tctttcttag acaagtcacc
    18901 atccataaca atagttctag aaatttgctg caaaggaaca cctaattgtt cagcagtttc
    18961 tgtaggaata gcataacgag ccatcaacgg gtggatgagt agtccagcaa tcactgtata
    19021 acccagcacc acttgaaaga atgcttgata ccttttttgt ttcaaaataa tcaagccgca
    19081 aatcaaacct gtgccggtta ctacataaat cccattattt cttagatagg tggtcagtaa
    19141 agcaaggatg attacgtaaa tctgattgag aatcgttggc ttttctcgtt gaaaaataaa
    19201 gtcaaaaacc gctaaggaaa ataaaaagca agcgatagag aacaaagtat ctttttgcat
    19261 gttcaaggcg tagattggaa aaaccggtgc aaatgcaaaa tagcataaac taatgagtac
    19321 cggaaaaaaa tggatgtttc tttttaacag ccagcaaaga gaataactta tcgctgaagc
    19381 tgttatcaat gtttgaaaaa tcgaaaataa aaatacaccg tgattgatcg tatcagttgg
    19441 taaatcatga acaaatagct tgaccagtaa ggtgaataag actggatgat gattgcttat
    19501 aggaacatca cccatagatt gggcgaccga acttaacgaa tcaggcaaaa ccactccagg
    19561 atacaatgcc aacagataag gaagccaaca cagaactaaa aagacagtaa agaataaaaa
    19621 atattttctt gaagagaaag cctgtgtgtt ttgagcagta aaaaatgttt ccagttttgg
    19681 cattgataaa taaatcgatt gtaaaaataa aaaagaatac acaaaaatgg agaaataaac
    19741 aagaaagtaa aaaatacttc catcagcaat cgttgtcttt gtatagtttg ctgagacata
    19801 ctgaaattgt atctgtctgc caatagaaag tgtaagagca aaagctgcag aaaaaaacaa
    19861 tcgggcaata gctgcaactt tgttttttat tgaaaacaac tctattccat attgcttaaa
    19921 agcaagtagc aaaaccatgg ctaaaccaat gattactgtt ggattcaaag ttcctagacc
    19981 ataagaagta aaaaaagcaa aacctgacgc aaataaagtt gcaaaaaaag taggaaatag
    20041 attctccttt ttcttatgca taaaaaaggc ctcctacttc atttctttat tttgatattc
    20101 gtggtagaca tggtataaat tcaattcata ttctttcttg gcatttgtga cgaccgtatc
    20161 caaaatcaac cctgttacta atgaaagaag ggctaaagtc atcaatccag tacttaaaat
    20221 cgcagaaggg atgcgtgtaa taaagcttgt cagcatatat tcacgaatca caggaatccc
    20281 catgaccaat ccaaataaaa agaagaagat ggtccagatc ccaaagaaaa gcaaaggttt
    20341 ataatctttg cacattttga tgatcatcat gatcactttg aatccgtcag agaatgtatt
    20401 caattttgat tcgctacctt caggacgatc gcgataatcg attggaatct caacgaactt
    20461 gaatttttta tctaaagcat gaatcgtcaa ttctgtttcg atttgaaaac cagaactcat
    20521 aatagggaag cttttgacaa aaattcgatt gaatcctcga tagccggtca tgacatcgcg
    20581 aatttttgtt ttatataagt aagtaattgt atttttaacc agattattcc caaaatcatg
    20641 gaaaggacgc ttattttcat caaaataggt tccatttgat agacgatcgc caatgaccat
    20701 atctgcttcg ccagaacgta atttctcaag caaaccatgt gcagcttctg caggataagt
    20761 atcatcacca tctaccatca aataataatc ggcatcaata tcaaaaaaca tttgacggat
    20821 tacattccct tttccttgac gtggttcttt ctttacgatt gctccgcctt gaacggccaa
    20881 ttcgtatgtt cgatcggtcg aattattatc atagacataa atatctgctt ctggtaattc
    20941 ccttttaaaa tcttcaataa cagttgaaat tgtcgcctct tcgttataac aaggaatgag
    21001 cacggcaatt ttttcacgtg attccatttg tcactctcct tatataataa aactaatctt
    21061 atttcaataa taccgtataa taactacaaa tactagctat tccaagagga aatagcccta
    21121 tttttataaa aatcacccac tataaaagaa ggataggata cctctctttt agatagtggg
    21181 tgcgtattcg ttatttcatt tttttgacgt attaatctat tgcgcccttt tttcttgaaa
    21241 tgtctaatac ggttcctgta tgagcatctg ctaaaaattc ataataaaca gtttcgccat
    21301 cttctatccg ggaaagccca ccccgataag cactcgaccg aacggcgaat ttccgaatag
    21361 gttctttttc aaaagagatc caagcacctt cgattgcgcc ttcttttaaa aaagatgttt
    21421 tgatattttc aagcacttgg tctgctgata acactttttt gcgatgatac caaatcgttg
    21481 aagctacccc actcaataat cctagaccga tcccaacggc taacccgcct ttaaaatagc
    21541 ttaattcgct ttcttcatac atgtggatat acctccttct tctcacttgc tactctttgt
    21601 gtttatttta ccacactctc atattttctt tgaaataaat aaccgctacc agaaaaaata
    21661 agctataata aatacgatcg gtctgcagaa aaaatcatat tttatccgca gatcaatata
    21721 agtctgagag gagtttagtc tgaaatttag acgaatagca tatggaagaa aaaacatttc
    21781 aacgaatcaa agagttaaca gaattacaag gaacaagcgg ttttgaacaa gacgttcgcg
    21841 cctatatgga aagtcatatg gaaccgctgg tagatgagtt acagttagat ggtttgggcg
    21901 gcatttttgg gctgcgccac catgaagaag cggatgcacc tcgtgtaatg gtcgcagcac
    21961 atatggatga agtcggattc atgctgacac aaatccaaga aaacgggctt tttaaagtcg
    22021 tacctctagg cggatggaac ccttacgttg tttctgctca acgttttaca cttaaaacct
    22081 ctactggtaa gaattatcca tgtatttctt cttccgttcc cccacattta ttacgaggaa
    22141 caagcggaca aaaatcagta gaagtaacag atattttgtt cgatgctggg tttgaatcaa
    22201 gagaagaggc aatgagttac ggtgttcttc caggagacac aatcgttcct tacgctgaaa
    22261 cgatcaaaac agccaacggg aagaatatca tcagcaaatc gtgggacaac cgctatggct
    22321 gtacaatggt attagaagct ttagaagcgt tacaaaatga aacattaggt catacattga
    22381 ttgcaggcgc gaatgtccaa gaagaagtag gcttgcgtgg ttctaaagct tctgtcaata
    22441 aattcaagcc agatctgttc tttgcagtag actgctctgc agcagatgac acagtgacga
    22501 aaaatggcac attcggacat ttaggagaag gtacattgat gcgtatccaa gatccaggtt
    22561 tgatcatgct tcctcgtttg cgtgagtact tgctagatat cgctgaaaca aacaacatcc
    22621 cttatcaata ctttgtttca aaaggcggta cagatgcagg tgctgctcat acacaaaatg
    22681 aaggaatccc aagtacagtg attggcgtag tgggacgtta tatccatacg catcaaacga
    22741 tgttcagtat tcgcgatttt gaagctgcac gagaaatgtt gattcaaaca ttgaaaggtt
    22801 tagataaatc aacagtgaac acgatcgtat acgggaaata acgcgatagt gaaactataa
    22861 aatctggcaa aagcaacctt ttgtcagatt ttttttataa aaagaggatc aatgtttgtt
    22921 agttcgaaag aaaggtgtat aatttaattg aaaaatacat aaacagaaag tagaggaaaa
    22981 agaatgatta tcccaaatag tttagaagaa ttagcaggct acgtagaaac tggaaaaagt
    23041 gtatttttct ttactgccgg ctggtgcgga gactgccgtt ttatcaaacc gcaaatgcct
    23101 gaaatcgaaa atgacttttc tgattggcaa tttattgaag tagaccgtga caagtacata
    23161 gatgtagccg ctgaatggaa catttttggt atcccaagtt ttgtcgtgat ccaagacgga
    23221 aaagaattag gtcgtctagt gaataaagat cgtaaaacaa aacaggaaat cgaatcgttc
    23281 ctagaaagtg taataaaagg ctgacatctg ttaggagggg tcaagatgaa tcaagaatat
    23341 aagaacattt tagtaggtat tgacggtagt gaacaagcac ttgctgcttt caaaaaagcg
    23401 ttagaagtgg ctcgccgtaa taatgggaaa gtatatgtag caaacgtaat cgatcagcaa
    23461 ttttataatt ttatgggcta tgctcctatt gatcaaaatc tagtcgatca gcaaactgaa
    23521 acagccaagc aaatgatcga agaatgtaaa gaatatggaa aatctgttga ttacacaaat
    23581 atagaaggaa ttattgcata cggctctgtt cgtgaatcca tggcagtcaa attgccaaaa
    23641 aaatatgcaa tcgatctaat catggttggg cagtctggac taaatgcagt cgaacgcttt
    23701 atgacaggaa gtgtagccag ctacgtcata cgtcattcac cttgtgatgt cttgatcgtt
    23761 tctgacgaag aagaacagcc aagtaattaa gcaagtggaa cttgtataac ctttttaatc
    23821 aaacagtaaa gggactgtga caaaagtctt gtcacagtcc cttgttacga ataaatggtg
    23881 ttccagaaga agcttcttcg gaaataaagc atgaaaaaaa tctcatgcag tgaatgcaat
    23941 tttttgttaa gataaatctc agcaaaggat aatggaggaa agaacatgat ttttgcttat
    24001 aacaaagcac acgtcggaga cactttgatg gttatcatag ccgacgataa aggaacagaa
    24061 aacactgtcg aacgtaaatg gaatgttgct cggatcaaag atgaacaagg tcaagttgtt
    24121 gcttggaact ttttccatat ctccgatcat ctaacaattg aagggaacgg ccaagtaagc
    24181 ttaacagatg aagaattagc gaaattgaat cgattgatta aagaagcagg attcgaagaa
    24241 cagcttgaag ctgatcatga accgaagatc gttgtcgggt atgtaaaaac atgcgtcccg
    24301 catcctgact ctgaccattt atcgattact gagacagaag tggacaatgg gacagtattg
    24361 caaattgttt gcggtgcacc aaatatcgaa gctggacaaa aagttgtcgt tgcaaaacca
    24421 ggagccatga tgccagatgg aatgatgatc tggccaggaa ctttgcgagg ggtagccagc
    24481 tatggaatga tttgttctgc tcgtgaattg catttgccaa atgctccaga aaaaaaagga
    24541 atcttagtat tgcctgagga tgccgtagtc ggagaagtat ttccaaaata aagaggttgt
    24601 gacaagtctt tgtcacaacc tctttattat taattttcac tattttgatt ttgttttaat
    24661 gccgattgat cgatcgtcaa ttcaacagta gttgtttttt ctttcgagcc ttcatagtaa
    24721 gtgattttga cagaatctcc cacttttttc ttatacaaag cagattgaag ttctacacca
    24781 gaagaaactt ctttgtcatc gatcttagta atgacgtcgt attgtttcaa cccagctttt
    24841 tctgccggtg tagctgtttg gacatttgtc acgatgacac cgttagttac agatgaagga
    24901 atcttcaaga tttgttcttg ttgctgagta gaaacggcag ataagtcgac cattgtgata
    24961 cctagagctg gacgtgtcac ttttccgtct ttttccagtt ggttgatgat attcactaca
    25021 tcgttacttg ggatggcaaa tcccattcct tctacgctta cattagaagt cgattcagaa
    25081 gtacttgcga tcttacttga gttgataccg ataacttgtc cttcgatatt cactaaagga
    25141 ccaccagaat ttcccgggtt gatcgctgca tctgtttgga tcgcattgat attgactgtt
    25201 tcgttcgatt cattcgtact tgttacttgt cggttcaagg aagaaatgat tcctgaagtg
    25261 acagaattcg catattcaga tcctaatggg gaaccaatcg caatcgcagg ttcaccaacc
    25321 ttcaaagcac ttgagtcccc aaaagaggca accgtttcga ctttgtctga attgatcttc
    25381 aacaccgcta aatctgaata ggcatctgtt ccaactaatt cagcttttac cttcgtaccg
    25441 tctttcatca tgacttccaa accttgctgt ccatctacta cgtgattgtt tgtgactaca
    25501 taagcagtat tcccgtcttt tttgtagatt acgccgcttc cttcactata agcttccaaa
    25561 ttactatcat ctgagctgct actttcttct tgctgcccga ataatccgcc aaaaccgctt
    25621 gattggtttt gactttgtaa gttgataaca gaaacgacag cgtcttgaac tttatctacc
    25681 gctttggtga tatcagaatc tacattgact ttgacatttt ccacaactgt ttcaccggca
    25741 gaattttgat tgccgcttgt agcagtagaa ctattgttgc cagtgcccat cgctgcataa
    25801 aaaatgccag ctgtcagtag tcctccaacg atccctccaa caagccccag acccaactta
    25861 cgccagaggc tattattttt attttttttc atctttggtg tcacattttt tctatccatg
    25921 tgttcctcct ccaattacat tcatttatct actgttagta tagtcttatt tgttgacttt
    25981 agtctacttt gagactttga actttttatg aaaaacttta ggaaaaatca ttgttttcag
    26041 atgaaaatat ttgattttct ttttccacag atattgtgta aaactacgcg gataggattg
    26101 gaaaaataag ggataaaaca tatccacagc ctgtgtataa agtggataaa ttcttataaa
    26161 acgtatgttt gcctaatgaa tgtgatgaaa taaggtaaaa caacgctgtt ttctgtggat
    26221 aaacctgtgg atgttgtgca taactgttgg ggaaatcttt ttaacaaaaa tagtcaaaat
    26281 aaacatatag acgaaaaaat tgattataat aaacaaagtg agcgtgaaga tcaaaggaga
    26341 gaaaacatga atatcaaaat tatttcagta ggaaaattaa aagaaaaata cttgatccaa
    26401 ggtatcaatg agtatgtcaa acggctgaat gcctatgcga aaatcgaatt gatcgaagtt
    26461 cctgacgaaa aagccccgga gaatttaagc gaagcgcaaa tgagacaagt aaaagaaaaa
    26521 gaaggagaac ggatcttagc gaaaatcaaa gaacaagaat acgtctatgg cttagcaatt
    26581 gaagggaaaa atcccacaag cgaatctttt gctaaacaaa tcgaccaatt aggtatccaa
    26641 ggaaaaagcc accttgtttt tgttatcggc ggttctttag gactcagcga agccgtcatg
    26701 aaaagaagca atgctcagat ctcttttggc aaaatgacgt atccacatca attgatgcgc
    26761 ctgatcctcg tcgagcaaat ctaccgtgcc ttccggatca acgcgggggc tccataccac
    26821 aaataatgat attttttaag agaaaaatag cataaaaatc tagttatccg cataaaaact
    26881 ggacttatca cactttatca aggtcaaaac cactcaattt actactaatt tactacttat
    26941 gaatgagctt tgatacgacg atttatcctt gaaaagtgaa gatataaaga tacttccaat
    27001 aaaatttgaa tatttaatag gtagacactt caaaaaatga ggtgtctatt tttttacccg
    27061 attttgaaag gaagtgaact tatgaaaaca aaaaatcaag aatcaaaagg tcgttcccca
    27121 ctctttaaga ccatcaaaca ttcattcagc caataaaaaa gaaaggatag gtaaaaatat
    27181 ggaacttaaa tttgtgattc ccaacatgga aaaaacattc ggcaatttag aatttgctgg
    27241 cgaggataaa gtcgttcagc gaagaatcaa cggacggcta actgtcttat caagaagcta
    27301 taatctctat tctgatgttc aaagagcaga tgatattgtg gtggtgcttc ctgctgaagc
    27361 tggcgaaaaa catttcggct ttgaggaacg tgtgaagtta gtcaatccac gtattaccgc
    27421 agagggctac aaaatcggca ctcgtggttt tacaaattac cttttacatg ctgacgacat
    27481 gataaaagaa taaagaaaga gaggaaaaat gatgagatta gcaaatggca ttgtattaga
    27541 taaagacacg acttttggag aattgaaatt ctctgctcta cgtcgtgaag tgagaatcca
    27601 aaatgaagac gggtcggttt cagatgaaat caaggaacgt acctatgact taaaatccaa
    27661 aggacaagga cgcatgattc aagtaagtat tcctgccagc gtgcctttga aagagtttga
    27721 ttataacgca cgggtggaac ttatcaatcc cattgcggac accgttgcta ctgccaccta
    27781 tcaaggagca gatgttgact ggtatatcaa ggcagacgat attgtgctga caaaggattc
    27841 tagttcattc aaagctcaac cacaagcaaa gaaagaaccg acacaagaca aatagtcgct
    27901 aggtagaaag gagacttttt cgcatgaaac agcgtggtaa aaggattcgc ccatctggta
    27961 aagatttagt ctttcatttt acgatagcgt cactcctgcc tgttttcctg ctggttgtcg
    28021 gactgtttca tgtgaagaca atccagcaga tcaactggca ggattttaac ctatcacaag
    28081 cagataagat tgacattccc tatttaatta tcagtttcag tgtcgcaatt cttatctgct
    28141 tgctggtagc gtttgtattc aaacgggttc gctatgatac ggttaaacaa ctttaccacc
    28201 gtcaaaaact ggcaaagatg atacttgaaa acaagtggta tgaatctgaa caggtcaaaa
    28261 cagagggttt ctttaaagat agtgctggtc gtacaaagga aaagataacc tacttcccta
    28321 aaatgtatta tcgacttaaa aatggcttga tacagatacg ggtggaaatc acgctgggaa
    28381 aatatcaaga ccaactctta cacttggaaa agaaattaga gagtggcttg tactgtgagc
    28441 tgacggataa agagttaaag gattcctatg tggaatatac tttgctctat gacaccatag
    28501 ccagtcgtat ttctattgat gaagtagaag ctaaagatgg taaacttcgc ttaatgaaaa
    28561 acgtatggtg ggaatatgat aagctccctc atatgttgat tgctggtggt acaggtggcg
    28621 gtaaaactta ctttatactg acactgattg aagccttgct tcatacagat tcaaaactgt
    28681 atattcttga cccgaaaaat gctgaccttg cggacttagg ttctgtgatg gcaaatgtct
    28741 actatagaaa agaagacttg ctttcttgca ttgaaacatt ctatgaagaa atgatgaaac
    28801 gtagtgagga aatgaagcag atgaagaact ataagactgg caaaaattat gcttacttag
    28861 gtctcccggc acacttctta atctttgatg aatacgtcgc tttcatggaa atgctgggaa
    28921 caaaagaaaa caccgcagtt atgaataagc tgaaacagat tgtcatgtta ggtcgtcaag
    28981 ctggcttctt tctaatactg gcttgtcaac gtccagacgc aaaatattta ggcgacggaa
    29041 tccgtgatca gtttaatttc agagtggctt taggtcgtat gtctgaaatg ggctatggca
    29101 tgatgtttgg cagtgacgta caaaaggatt tcttcttaaa gcgaatcaaa ggtcgtggct
    29161 atgttgatgt aggaacaagt gtcatatcag agttttatac tccccttgta ccaaaaggat
    29221 atgatttctt ggaggaaatt aaaaagttat ccaacagcag acagtccacg caggcgacgt
    29281 gcgaagcgga agtcgcaggt gtggactgat cttgctggct ggtgtggcaa tagccacgcc
    29341 agcacttaac ccctcggtat ctaacagggg ggtacaaatc gacaggaaac agtcaaaaaa
    29401 acattagaaa atcctttggt tacaagggat ttacaaaatt tcagcgtatg tcaaatgggc
    29461 tttaaaagtt gacatacgcc tttttgattg gagggatttt tactgaatga acaaacttgg
    29521 ttacagcatt taaaagaaaa acgcttggct tatggactat ctcaaaaccg tttagctgtt
    29581 gcgactggta ttacaaggca gtatctaagc gatattgaaa caggaaaagt caagccatca
    29641 gaggatttac agcagtccct ttgggaagct ctggaacgct tcaatcccga cgctcccctt
    29701 gaaatgctgt ttgattatgt aagaattcgc tttccgacaa cagacgtaca gcaggtggtc
    29761 gaaaacatct tacaactgaa actgtcctat tttcttcatg aggactatgg tttctattct
    29821 tattcagagc attatgcttt aggcgacata ttcgtccttt gctcccatga actggacaaa
    29881 ggagttctgg tggaattgaa aggtcgtggg tgcagacaat ttgaaagcta tcttctggca
    29941 caacaaagaa gctggtatga gttctttatg gacgttttgg tggctggcgg tgtgatgaaa
    30001 cgccttgacc ttgccattaa cgataagaca gggattttaa atatccctgt actcactgaa
    30061 aagtgccaac aggaagaatg tatctccgtc ttccgcagtt ttaaaagcta tcgcagtggc
    30121 gaactggtac gcaaagagga aaaggaatgt atgggaaaca ccctctatat cggttcatta
    30181 caaagtgaag tttatttctg tatctatgaa aaggactacg agcagtacaa gaaaaatgat
    30241 attcccattg aagacgcaga agtaaaaaac cgttttgaga ttcgattgaa aaatgagcgt
    30301 gcctattatg cagtccgtga tttactcgtc tatgacaatc cagagcatac cgcctttaaa
    30361 attatcaatc ggtatatccg ttttgtagat aaagacgatt ccaaacctcg ttctgattgg
    30421 aaactgaatg aagaatgggc ttggtttatt gggaacaatc gtgaacgatt aaaactaacc
    30481 acaaaaccag agccttactc cttccaaagg acgctgaact ggctatctca tcaagttgcc
    30541 ccgaccttaa aggttgcgat taaacttgat gaaatcaacc agacgcaggt tgtaaaagac
    30601 attctcgacc atgcgaaact gacagaccga cacaagcaga ttttgaagca acagtcagta
    30661 aaagaacagg acgtgataac aacaaaaaaa taactcaaat acaaattcat tgaatataga
    30721 gaggagaaca tttttatgaa ttttggacaa aacct
//
LOCUS       DACEUZ010000018        30177 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.018, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000018 DACEUZ010000000
VERSION     DACEUZ010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30177
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.018"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(<1..737)
                     /locus_tag="IXG44_000722"
     CDS             complement(<1..737)
                     /locus_tag="IXG44_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ1657460.1"
                     /translation="MRYSTILISTFAALSLSLTINQTVHTEEIETAKWTETKWANVNR
                     ITFQDEFSSFNSGVAKLRKGQTATIKAKIDYSGDLAPILQSAVTFENVDPALSIGYDE
                     NTLTIQHKQAIFTFTVTLNQDLLKPALFTVKVSDGLPNDHHHLTPYSQRQTIEQDSAI
                     DSGGDLVEEPTDKPENENKPEVPPTENPDGEQKPEIEPGEEPDTETQPEPDNESKPEI
                     TPGEKPDVDPEEKPDVTPEPDTDSGNQT"
     gene            885..2438
                     /locus_tag="IXG44_000723"
     CDS             885..2438
                     /locus_tag="IXG44_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ1657461.1"
                     /translation="MQTQEAIKTFLLKKKSNTKLDIFLFLSEHRFFITTQYLADHFHM
                     SESNFLLYIKELEQDFERLNLTELHIDKQKPFLKLNFEGIDPAYCYYRLFGRYCNESV
                     SYQILTSLFSCQTNSIISLSQQTNYSASYLYTKMKKINAFLALYGLSISFSNNGRKSF
                     SGKEVQIQYAALDIYWNIFSSTATEFYDEDPQVVAFMMNTFFKKEVLDRLNSGMIDKL
                     YFLLKICKENFPATSVKDVQKEFTEYEYIDFFIDPSVDILRPNLPICREQRILVNILA
                     RLSITKIESEETSLEQYKLLKKANVPHVLYSREMVESFCTVFDLFIPEKEKILYSLAF
                     ARNKLYNGFLNFNQPNTPLPTFMLYQEHSVLKEVQTAIEQFYMDFREQNQHLLPVILE
                     KENVQWMVEDLVHLYDRYKKQPRIVIGVNYTRDFYISKDLIVKIEQIFSNESIKIQND
                     FMENCNIVISDCPLKQLPSHIKKIYILKGIITPEDWKKVIMRISEYIFELKEEALMDQ
                     SKKENQVTS"
     gene            complement(2546..3628)
                     /gene="uxuA"
                     /locus_tag="IXG44_000724"
     CDS             complement(2546..3628)
                     /gene="uxuA"
                     /locus_tag="IXG44_000724"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAQ1657462.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFELPGWEP
                     ERLAHIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLP
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(3619..5265)
                     /locus_tag="IXG44_000725"
     CDS             complement(3619..5265)
                     /locus_tag="IXG44_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAQ1657463.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAALLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPDMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDFQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            5449..6111
                     /locus_tag="IXG44_000726"
     CDS             5449..6111
                     /locus_tag="IXG44_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1657464.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            6255..6818
                     /gene="ahpC"
                     /locus_tag="IXG44_000727"
     CDS             6255..6818
                     /gene="ahpC"
                     /locus_tag="IXG44_000727"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HAQ1657465.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            6828..8357
                     /gene="ahpF"
                     /locus_tag="IXG44_000728"
     CDS             6828..8357
                     /gene="ahpF"
                     /locus_tag="IXG44_000728"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HAQ1657466.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     RTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            8514..8864
                     /locus_tag="IXG44_000729"
     CDS             8514..8864
                     /locus_tag="IXG44_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HAQ1657467.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            8963..9493
                     /locus_tag="IXG44_000730"
     CDS             8963..9493
                     /locus_tag="IXG44_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1657468.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            9647..10798
                     /locus_tag="IXG44_000731"
     CDS             9647..10798
                     /locus_tag="IXG44_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAQ1657469.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(10907..12946)
                     /locus_tag="IXG44_000732"
     CDS             complement(10907..12946)
                     /locus_tag="IXG44_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HAQ1657470.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEQPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSYQVTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLINPIQQAAMYSVFANNGTLVY
                     PKLIADKETKDKKNVIGETALQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGKENSFLFAFNPDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(13004..13759)
                     /locus_tag="IXG44_000733"
     CDS             complement(13004..13759)
                     /locus_tag="IXG44_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAQ1657471.1"
                     /translation="MKVSQKIILSILLLLTLVVGYFSIEFIHGFSSAKQSSTVKKVEP
                     KSVPTTLNVALIGSDARSKDENGRSDSLLVAQYNQKTQQAKLISIMRDSYVDIPGYGM
                     NKINAAYSYGGVDLLNQTLKENFKFEAPYYASITFQDFIDCVNELFPGGVKIDAEKSL
                     DLDGVYINKGEQVMDGNTLLQYARFREDKEGDFGRIRRQQQVIKAISQQLKDVTSIFK
                     LPKAVGKLLGSIQTNLPESVLLDCGMDFLKDIC"
     gene            complement(13851..15014)
                     /locus_tag="IXG44_000734"
     CDS             complement(13851..15014)
                     /locus_tag="IXG44_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAQ1657472.1"
                     /translation="MKKRKKIDWWILGPYLTLSMIGLLEVYSASSYRLLQADENTKSL
                     LLRQLIFIFLSWSVIFLARSVKLHYLLHPKIAGYGLALSIFFLVLVRIGIFGVTVNGA
                     QRWISLFGIQFQPSELANLFLIFYLSWFFRDGNSSPKDLKKPFLITVGITFLILFQPK
                     IAGALMILSIAWVIFWAAAVPFKKGIYLIVTFSALLIGAAGGVLYLGNKGWLPQMFNH
                     AYERIATLRDSFIDSHGAGYQMTHSFYALYNGGIFGRGLGNSITKKGYLPETETDFIF
                     SIITEELGLIGALCVLFLLFSLCMRIFCLSSRCKNQQAGLFLLGFGTLLFVQTIMNVG
                     SIAGLMPMTGVPLPFVSYGGTSYLILSLGIGITLNISSKIQAEELPLYRPEKQ"
     gene            complement(15531..15737)
                     /locus_tag="IXG44_000735"
     CDS             complement(15531..15737)
                     /locus_tag="IXG44_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1657473.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            complement(15753..16367)
                     /locus_tag="IXG44_000736"
     CDS             complement(15753..16367)
                     /locus_tag="IXG44_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HAQ1657474.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(16514..17197)
                     /locus_tag="IXG44_000737"
     CDS             complement(16514..17197)
                     /locus_tag="IXG44_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAQ1657475.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RSNTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(17306..17764)
                     /locus_tag="IXG44_000738"
     CDS             complement(17306..17764)
                     /locus_tag="IXG44_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HAQ1657476.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(17848..19611)
                     /locus_tag="IXG44_000739"
     CDS             complement(17848..19611)
                     /locus_tag="IXG44_000739"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HAQ1657477.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(19832..21292)
                     /locus_tag="IXG44_000740"
     CDS             complement(19832..21292)
                     /locus_tag="IXG44_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1657478.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(21500..22426)
                     /gene="glsA"
                     /locus_tag="IXG44_000741"
     CDS             complement(21500..22426)
                     /gene="glsA"
                     /locus_tag="IXG44_000741"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HAQ1657479.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(22449..23876)
                     /locus_tag="IXG44_000742"
     CDS             complement(22449..23876)
                     /locus_tag="IXG44_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1657480.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(23907..25121)
                     /locus_tag="IXG44_000743"
     CDS             complement(23907..25121)
                     /locus_tag="IXG44_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HAQ1657481.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            complement(25462..25704)
                     /locus_tag="IXG44_000744"
     CDS             complement(25462..25704)
                     /locus_tag="IXG44_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ1657482.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            complement(25736..26293)
                     /locus_tag="IXG44_000745"
     CDS             complement(25736..26293)
                     /locus_tag="IXG44_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ1657483.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            complement(26306..26494)
                     /locus_tag="IXG44_000746"
     CDS             complement(26306..26494)
                     /locus_tag="IXG44_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAQ1657484.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            complement(26507..27073)
                     /gene="amaP"
                     /locus_tag="IXG44_000747"
     CDS             complement(26507..27073)
                     /gene="amaP"
                     /locus_tag="IXG44_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAQ1657485.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            28303..28776
                     /locus_tag="IXG44_000748"
     CDS             28303..28776
                     /locus_tag="IXG44_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HAQ1657486.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            28785..29012
                     /locus_tag="IXG44_000749"
     CDS             28785..29012
                     /locus_tag="IXG44_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1657487.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            29648..30019
                     /locus_tag="IXG44_000750"
     CDS             29648..30019
                     /locus_tag="IXG44_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1657488.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
BASE COUNT         8984 a         5808 c         5182 g        10203 t
ORIGIN      
        1 gtttggtttc cagaatccgt atctggttct ggagtcacat ccggtttttc ttctgggtct
       61 acatctggtt tttctccagg ggttatttca ggttttgatt cattgtctgg ctctggttga
      121 gtttctgtat cgggttcttc tccaggttct atttccggtt tctgttctcc gtctggattt
      181 tctgtaggcg gtacttctgg cttattttca ttttctggtt tatctgttgg ttcttcaaca
      241 agatctcctc ctgagtcgat agctgaatct tgctcaattg tctgccgctg actatacggc
      301 gttaaatgat gatgatcgtt cggtagtcca tctgatacct tgacagtaaa aagcgctggt
      361 tttaacaagt cctgatttaa tgtaacggtg aaggtaaata tcgcttgctt gtgttggata
      421 gtcaaagtgt tctcgtcata gccaatcgac aaagcgggat caacattctc aaatgtaaca
      481 gctgattgta gtataggtgc taagtctcct gaatagtcaa tttttgcttt gatggtcgct
      541 gtttgtcctt ttcttaattt tgcgactcct gaattgaagg atgaaaattc atcctgaaag
      601 gtaattcgat tgacgttggc ccacttggtt tctgtccatt tggctgtttc tatctcctct
      661 gtgtggactg tctgatttat agtaagcgat aagcttaagg ctgcaaatgt tgatattaag
      721 atggttgaat atctcataac tctctcactc cttgattttt ttgtatgcaa gaaataagat
      781 agtagagatt ctacctatat gtttactgtt tttttgtcta aatttatgaa aaaaacagta
      841 atattttcaa gttttaacaa tttaagtaaa aaggagcctc tttcatgcaa actcaagaag
      901 cgattaaaac gtttctcttg aagaaaaaaa gcaatacaaa attagatatt tttcttttcc
      961 tatctgaaca tcgtttcttt attactacac aatatttagc agatcatttc catatgtctg
     1021 aatcaaattt tttactatac atcaaagaat tagaacaaga ctttgagcgt ctcaatctaa
     1081 cagaactgca tattgataag caaaaacctt ttttaaagtt gaattttgaa ggaattgatc
     1141 cggcttactg ctattatcgt ctatttggaa gatactgcaa cgaatcagtc agttatcaga
     1201 tcttgacttc tttgttcagc tgtcagacaa attcgattat cagtttaagt cagcaaacaa
     1261 actatagtgc ctcttatttg tatacgaaaa tgaaaaagat taatgcattt ttagcattgt
     1321 acggtttgtc tatctccttt tctaataatg gcagaaagtc tttttctgga aaagaggtac
     1381 aaattcaata tgcagcatta gatatttatt ggaacatctt ttcaagtact gctacagaat
     1441 tttatgatga agatccgcaa gtagtggctt ttatgatgaa tacttttttt aaaaaagaag
     1501 tacttgaccg actaaatagc ggtatgatag ataaattata ttttctatta aaaatatgta
     1561 aagagaattt tcctgcaact tctgtgaaag acgtacaaaa agaattcaca gagtatgaat
     1621 atattgactt ttttattgat ccctctgtag atattttgcg acctaatctg ccaatttgtc
     1681 gcgaacaacg catcttagtg aatattttag ctcgactttc tatcacgaaa atcgaatcgg
     1741 aagaaacaag cttggagcaa tacaaacttt taaaaaaagc aaacgttcct catgttcttt
     1801 attcaagaga aatggtggag tccttttgta ctgtatttga tttgtttata cctgaaaaag
     1861 aaaagattct ttactcattg gcattcgcac gaaataaact ttataatgga tttcttaact
     1921 tcaatcaacc aaatacgccc cttcctactt tcatgcttta tcaagaacat agcgtgttga
     1981 aagaagtcca aaccgccatt gaacaatttt atatggattt cagagaacaa aatcaacact
     2041 tgttaccagt tattttagaa aaggaaaatg tgcaatggat ggttgaagac ctagtacatt
     2101 tgtatgatcg ctacaaaaaa cagccgcgaa ttgtgattgg tgtgaactat acgcgtgatt
     2161 tttatatcag taaagatctg atcgttaaga tagaacaaat tttttcaaat gaatcgatca
     2221 aaatacaaaa tgattttatg gagaactgca acatcgttat cagcgactgc cctttgaaac
     2281 aattgccttc tcatatcaaa aaaatctata ttttaaaagg aatcatcaca ccagaagact
     2341 ggaaaaaagt gattatgagg atctcagaat atatctttga attaaaagaa gaagcactca
     2401 tggatcaatc gaaaaaagaa aatcaagtta cttcctagac caaataagat acataaatcc
     2461 gaatgcttat taacaacctc cggatttatg tatcaagaat ttgaacatcc ttcttattta
     2521 cataataaaa ttacagtttt tttcactaat tatctttact tttgtttcct ttttcgattg
     2581 cttcccataa tccatttaga taaacagcac ctaatgctcg atcatataag ccgtatcctg
     2641 gtcttcctgt ctcattccag atcattcttc catgatcagg acggatgtat ccgttaaatc
     2701 cgttgtcata cattgttttt agtatgcggt acatgtctaa tgaaccgtat tcggatttat
     2761 gcggtgcctc atagaaatct cgattagcta aaaatttaat atttcgaaca tgtgcaaatg
     2821 ggattcgatc tctttgacaa aattctgcta aaatcccata tacatcattt tctggatctt
     2881 ctgcaatact ccctgtacaa agagtgattc cgttataagg gctgtctact atgcggcaca
     2941 accaatctaa atcttctcga ttctttacga ttcgaggtaa tccaaaaatc gagtatggcg
     3001 gatcatctgg atgtacagcc attttgaccc ctacctcttc acatgttggg atgattgatt
     3061 ggagaaaata ggcaaaattt tctcgaagtt tttcttcgtc tactgatgca taggcttcaa
     3121 gcaaggattt gatatgagct aagcgctcag gttcccatcc cggcaattca aattcattgc
     3181 tggaatgttc gatgcgcgcg acgatttctc ttggatcatc tggaatatca tcagatataa
     3241 aagctaacgt ggatgagccg tcttctaagc ggtagtcaag gtcagatttc acccagtcaa
     3301 atacaggcat aaaattatag cagatgactt ctattccata ttctgccaag tttcggattg
     3361 tctgcttata gttttcgata tactgctcac gggaaggtaa tccaatttta atggcatcat
     3421 gcacattgac gctttcaatt acttttagtg ttaacccacg agactcaatt tcttctttta
     3481 gttctttgat cctctcccta ggccatacct ctcctactgg tatatccatc aatgtgccta
     3541 caatcccttt gaccgcagga atctgtctga tttcatctaa ggtaatcttt tcttcctgtg
     3601 atccgtacca tctaaaagtc atttccatga taaatttcct ccatgttctt gtaagtaatc
     3661 ctgtaatgtt tttcgtacgg cattcggtcc tgctaccatc ttgatgaaca tttcttctat
     3721 ttttctgcca agctctgctt ggtaaagatc tacaccaaaa atttctttat ttgacaaaat
     3781 aggagccatt ttctcatgaa tatgtgatgt atcttgcgta cccagttgaa tatcggaaag
     3841 cttatgctga aagtctgtca atagtgggtc tggactcggg atgaatggtt ttccttgatc
     3901 atcaatgcca ataaggtatc gaagccaagc agcgagagtc aatggtatga atacaagtgt
     3961 ctgcgggtct ttaacttcta tttccatata ttttttaatc gtctcaccat aacgaatcgc
     4021 caccttttga gaggtatccg tggcgattcg ctgaggcata tcaggaatat ttggattaga
     4081 aaagcgcaca gtaattactt cttccagaaa gatctttgga tcaatgatac ctggagactc
     4141 cacaactggg aggccttcat tgtacccgat ttcagaaact agcagcgata aatctggatc
     4201 tttcatctcc tcagctatgc tcctataccc cagtaaacaa cccataatag ctaaagacgt
     4261 atgcaaagga ttaaggcaag tcgtcacctt catcgtatct gctttatcta ccgttgcacg
     4321 atcagtcaag aatactccag ctttttcgaa tttcggacgt ccattgggaa aatgatcttc
     4381 catgattaaa taatggatgt cctctgtatt gacaaatgga gcaatattat ttccttttgc
     4441 ggtttggaaa atcgacatat ttgaaaagcc ctcttttgcc aacattttgc taactgacaa
     4501 ggaaggattc ggggtgatac gatcaatcat cgtccatgga aacgcaacaa tttcttcatt
     4561 cgataagtat tggataaatg tattttgtac ataaccttcc ttttgccatt cttttgcgat
     4621 cgttagcaaa gaagtacgta attgactacc gttttgtgaa aaattatctg tagagacaag
     4681 tgcaatcggc caagctccag ataaaaaacg tttataaagt agcgcagcaa gaatactcat
     4741 tgtatgtgat ggcttctttg gaccattttt tatatcgact gcaatcggtc ctatatattt
     4801 attatttgta tctttcaatc gataaccttt ttcagtaata gcaaaagtaa ctaattgtaa
     4861 actaggattt tcaaagtgtc gaatcatctt atcaaagtgt tcagaacaat ctggatgaca
     4921 atacagactg tcagctgtcg cagctaatat acgtttgacg aactctccac tgcttggcat
     4981 gaccacttca agaatcaaat catcaaaatc ttggtaagcc ctttcaatta tttcttcatc
     5041 gaatgtttca caaacaatga caccagtgga caattcgcct agatccgcta aacgttgagc
     5101 gatttctgca tgaaatcctc gataaagatt tccgccaccg aaatggatcc agacaggatt
     5161 tttttgtcca ttttctctta atgatgcacg gtcaaaatga ggtatttcta cttctttttg
     5221 agataattca gggataaagt tcctccagtt gtttaaatca atcatttttc ccctcctaaa
     5281 ctaacatatt agtttttgta ttcaagtgaa ttttatgcaa taaaaatcgc tatgtcaacc
     5341 aaaattttac tatttataca aatttactag ttaatgtgac ataccagatt gtttttcaga
     5401 cctcttctgt tatactacgt tcatagaaat gattttggag gaataaagat gaaaagcagc
     5461 ttgcaatctc aagcttatca agcgattcgt aaacaaatta tctatgctga cttagaacct
     5521 ggaaggaaaa tatctgaaaa ggcactggag gaacatcttg ctatcggccg gactcccatt
     5581 cgcgaagcat tgattcaact acgtcagcaa gaactcgtgt atacgatacc tcaaagtggt
     5641 acgtatgttt caaaaattaa tcttgtttct gcagaaaatg ctcgctttgt ccgagaacag
     5701 ttggaagagc aaatcatgat tgaatgttgt tccaaattgg acacacaaac acgaatagtg
     5761 ctagaaacga ttttagaaga acaagaaaaa gcagtcaaaa taaatgacga acgcgctttt
     5821 ttccgtgcag ataatttatt tcatgaagct tgtttcgaaa ttgcaggaag aaaagaagtt
     5881 tggaactggt tagatgacca taatacccat ttagaacgtt tccgctggtt acgagtgacc
     5941 attcaggggc ttccatggga aacgatcatg gaacaacatt atgatttact caaggcttta
     6001 gttgataaaa aaccagaaga agctcgattt caaacgggat tgcacttaca catgatgctg
     6061 aaagaacagg aaactgttac tcaacgttat cccgagtatt tccagcagta gcattttctt
     6121 gcgacctctt ctatttttat aattaacctt cgtttttgag taatctttat taaataacaa
     6181 aatatccatt gactttgcaa ctccaactaa attacaataa ttataaataa ataattatta
     6241 ttggaggttt tactatgtca ttgattggga aaaaaatcga agaatttaat gcacatgctt
     6301 atcatcaagg agaattttta gaagtcagtg aaaaagatct tatggggaac tggagtattc
     6361 tttgcttcta tccagctgat tttacttttg tttgtcctac tgaattagaa gacttacaag
     6421 aacaatacag tacattgaaa tctttaggtg ttgaagtctt ttcttgctct accgataccc
     6481 actttactca caaagcatgg catgatacat cagatgctat cggtaaaatc gaatacgtga
     6541 tgatcggtga tccgtctcat cagatttctc gtatttttga tgttttggat gaagagcaag
     6601 gattagctca gcgaggaaca tttattattg atccagacgg aatcgttcaa gctatggaaa
     6661 tcaatgcaga tggaatcggt cgtgacgcta gtgcactgat tgataagatt cgcgctgctc
     6721 aatatgtacg tacccaccca ggtgaagttt gtccggcaaa atggaaagaa agtggcgaaa
     6781 cattgaaacc aagttttgat cttgttggaa agatttaggt ggtggatatg ttagacaaag
     6841 aaactaaaca aaatctcgaa caatatttag cactgattga atctcctatt gttttttctg
     6901 ttagtttgga taatagtgaa aatagtcaaa aactagctga atttacgaag gaaatagcag
     6961 aaatgtctcc taaaatctct tgggaaaaaa gagattcgat gcgcacacct agtttttcta
     7021 tcaatccagt tggcaaagaa agtggaatcg tttttgctgg tatccccctc ggtcatgaat
     7081 tttcctcttt tttactggct atgttacaaa ttagtggacg ggcaccgaaa atcagtgaat
     7141 ctttgagtct taaaatcaaa aaaataacac aaacattgca ttttgacagt tatgttagct
     7201 tgacttgtca taattgcccc gacgttgtac aggcattgaa tatattagct gtattaaatc
     7261 caaatattac tcatacgatg attgaaggtg ggatgttcca aaaagaaatc gatgataaaa
     7321 aaatcatggc agtgccaact gtgtttttaa atggagaaga atttagtagc ggacgcatga
     7381 cgatcgaaca aattgttgaa aaaatcactg gccctttgat tgaagaagag ttgtttgaaa
     7441 aagaacctta tgatgtatta gtgattggcg gtggaccagc agctagtagt gcagctatct
     7501 atgcagcacg taaaggaatc cgtacaggac ttgtcgctga ttctttcggc ggacaagtcg
     7561 ttgaaacact agggattgaa aatatgattg gtacctctta cacagaaggt ccaaaattga
     7621 tgcagcaagt agaaagtcat gtgcgtagtt accctatcga cattatgatg aatcaacagg
     7681 ccgtttcctt aaataaagag caacatttta ttaatattgg cttagctaac ggtatatcac
     7741 ttaaagcaaa aaccgccatc atcgcgacag gtgcgcactg gcgttctatc aatgtaccag
     7801 gcgaaaaaga gtttaaaaat aaaggaattg cttattgccc acactgtgac ggccctttat
     7861 ttaaaggaaa agagattgct gttatcggtg gtggtaattc tggtgttgaa gcagcaatcg
     7921 atttggctgg gttagcaaaa catgtatatg ttctagaatt cttaccagaa ttaaaagcag
     7981 atcaaatctt acaggataaa ctagtctctc ttgcgaatgt cactgttatc aaacatgcag
     8041 cgacgaaaga aatcagcggt acgaatcagg tagaatctct ctcctatatc gatcgccgta
     8101 cgatggagat gcatacacta gctgtttcag gagtattcat tctagtcggc ttactcccta
     8161 atacggattg gctagataga acgatcgaaa tgaatccaag aaatgaaata gtgaccgaga
     8221 aaaatggcgc aacgaatatc cccggcgttt tcgcagctgg tgattgtaca gatagtccat
     8281 acaaacaaat cattgtttca atgggttcag gagctactgc agctttagga gcattcgatt
     8341 atctgatgcg cacttgagct atgaagattg taaaaagtca gagagcggaa aaaaggatgg
     8401 tgacaagaat tttgtcacaa tcctttttgt ttttttgaat aatcgctttt tcttctcttt
     8461 ttcttatata ctataagtag taaataattt gaaaaaaaga aaggtgactg attatggcgc
     8521 aaaatcgtat ttttacgaca agttttgcaa gcgtttatcc tatgtatgta gcaaaagcgg
     8581 agagaaaaga tcgtataaaa gaggaagtag atgaaatcat ttgttggctg acgggttatt
     8641 cccaaacaga actagaatat caactacaaa aacaagtgac tttcgaacaa ttttttgcgg
     8701 aagcccctgt ccccaatcct tcacgttcct tgattacagg tgttgtttgt ggtgtacgag
     8761 tagaagacat cgaggataac ttgatgaaag aaattcgtta tttagataaa atgatcgatg
     8821 agctagcaaa aggcaaaaaa atggaaaaga tcctcagaag ttgatttttc cagagatatt
     8881 cttctccttc gctcattttg taacatttaa tttggtaaaa tgactagttc acgttaaaat
     8941 aagtcaaagg aggttttctt atatgtttga gacgattaaa caaaatattc aaaaatacgc
     9001 cttattaaga gctgctgttt atattatttc tgggattgcg attgtgctaa acccttctgc
     9061 tgtttttcat tttatcggtt atctgatcac cgcctatctt gttttgcttg ggttgttgaa
     9121 tctttttgag gcttaccgtt ttaaaaggaa aacaggttct tggagtatcg gtctgttcag
     9181 tggtcttttt ttcctgatct tagctctgat cgtctatgct tttgcacctg caatcgtttc
     9241 tatcctgccg attcttcttg gattagctgt tatcatcaac agcttgttcc agttgttctt
     9301 cagcatgaat acaaaagcta aaagttggag tgtttatagt agtattctgt tgttgattgg
     9361 cggattcgtc ttattgttca atccattcaa aagtttgatg cttctattcc aaatattcgg
     9421 ctttattttg attttcatgg gcatccagga gattatcaat ttcatcagaa taagaaaaat
     9481 gactatacgt taatcatttt tagttattaa aatagaccgt ataaaaacca acaatccaaa
     9541 aaaatctttt ggattgttgg tttttttgtg agtatatttt cctatttttt caataatgta
     9601 tgttatgcta agttcgggct atattttata caaaggaatg atgagaatgg aatttattgg
     9661 tattttatgc ctgatattga tttcaacaac gattggctca catatctcgc ggagattagg
     9721 tatcccagca gtgattggac aattacttgt tggtgttttg ctaggccaag ctggattgaa
     9781 ttgggtcaaa cctgatattc tagtccatga cttttcggag attggtgtga tcttattaat
     9841 gtttttagct ggtctggaaa gtgatttatc tcttttgaaa aaatatttcc gacctggtat
     9901 gttcgttgct ttgcttggga tcatcttccc ggttgttttt ggctggttga caggcaaagc
     9961 attccaagtt tcgggaaatg aagcgatttt ctttgggatt attcttgcgg caacttctgt
    10021 cagtatttca gtagaagtat taaaagaact aaacgtagtg aatacaaaag aaggatcaac
    10081 aattttgggt gcttctgtgg tagatgacat tttagtcgtt ttagttctaa gtttcagcct
    10141 atcttttcta acgggggaag cttctagcaa tgcttcttta ccgcttgttt tgattgaaca
    10201 gctaatttat tttgttttta ttttcttatt agtcaaatgg attgcgcctt tcctactttc
    10261 atttgcagaa aaagtgtacg caaattcttc tattatcatc gtatcgctag ttatttgttt
    10321 aggaatgtct tacgtagcgg atcttgttgg gcttagctct gtcattggtg ctttttttgc
    10381 tgggatagct gtcagtcaga caaaggttaa agaagaagta taccacaatg tagaggcttt
    10441 aggatatgca gtatttatac cagtattttt tgtcagtgta ggattagaag ttgatttttc
    10501 tgactttagt gaacaactac tctttatttc tatattgacg cttgtagcga ttttgaccaa
    10561 attgcttgga ggttattttg gtgctcagat tgccggtttt tctcaacata gttcattgat
    10621 ggtcggtgct ggtatgattt cgcgtggaga aatggctttg atcattttac aaattggaca
    10681 gcaaagtcaa ttgatcgaac cacagtacta ttcaccttta gttatcgtga ttttattaag
    10741 tacacttatt tctccacttg ttctcaaata ttttacaaaa aaagtttatg aagtgtgagt
    10801 aaatcaaaaa gaagttgtga aagagacatc gtgaaaaccg tttatttgaa aaaaggactg
    10861 tgacgataac gccacagtcc ttttactgta cttaaaaagc aattttttat tgataatttt
    10921 ggttgaggta ttgcaatagt tccgatgctc gtttggttgc tgaatcatca tcttctttat
    10981 tttccaacat gctgaccatc atataacctt ggttatcagg attgaaagca aacaagaaac
    11041 tgttctcttt cccctttaca tcctgttttt ctttgatttc cgctgtacca gttttcgctg
    11101 ccaacggaat ccctaaagca gaaagagaat gtgctgtacc atttacatct tgcacaactt
    11161 ctctcagatc tggcacgatc gtttgtaatg ctgtttcgcc gatgacattc ttcttatctt
    11221 ttgtctcttt atctgcaatc aatttaggat agacaagtgt gccattgttg gcaaaaacag
    11281 aatacattgc tgcttgctgg ataggattaa ttagaagttc gccctgtcca tatccagtat
    11341 cagctagcaa gatatctgag ttaaagctat cttcattaga aatttgtgct ggattcatac
    11401 taattggcaa atcaaggtct tcaccaaaaa tgaatttatc taagcctgta cgaaattttt
    11461 tctcacccat tttcaacgtt tcttgtgccg catatatatt atcggaatag atcaaagcag
    11521 tttttaagtc tacttgtgat acatcactac tgacacgcgt tacttgataa gatccccaag
    11581 aactgtcttt ttgccattta agcccgttga tcgtcaacac ttcatttgga tcgatagtgc
    11641 cgttgtcgag accgattgct gctgtaatca ttttaaacgt agatccagga gcataacctg
    11701 tcgcaaatcg gctgatgaat ggctgttcag gattttcttc ataagctttg taatcttctt
    11761 gtgagatccc gtttgtcatt ttgtttggat catagcttgg agagctagca agcgcaagaa
    11821 gatcaccggt ttttggcgtt gtcgcaacag ttgatccagc ttttcctcct agactgtcaa
    11881 aagctgtttt ttgtgccttc gcatcgattg tcaatttgat atctttgcca ttttgaactt
    11941 catgttcgat cagaactttt ttctctacac cgtctgcatc tgtaatgctt aatttcccac
    12001 ctgttgtccc acgaagatcc ttatcaaaag ccatctccaa acctgagcga ccgatttttc
    12061 cattactgct taattctgga ttcttatcaa tatcttctgc tgtgatatct cccacgtaac
    12121 caatcagctg agcagctgct tcacccaaag gataatatct gccgtctact tcttgaatgg
    12181 tagctccagc tggaagttct ggcgttgctc catcaatgat tttcaatggg acaaagtaat
    12241 cgggttgtac ccagctttgt gaaatcgcct gattgatagc atcttctgtt aagtcgaagg
    12301 aggaagcaat cgctttgata ttggctgttt tttcgcctcc atctccaagt ttacttggaa
    12361 cgactcctaa ttgttttagt ttgcctgttg ttgctaatgg ttcaccattt cgatctataa
    12421 tattccctct tgctgcttct tgtgtggtca ggcttacttt gtcatttcct tccatttctg
    12481 gaaaaaccaa gttgggttgc cagttgatcg tggtttgacc atcatttctg tctaatgttc
    12541 ctttataaga aagatctttc aattcaccta atgaggtatt catctttgct ttatatgaaa
    12601 aactgtaagt agaatcgtct tttttatcta cttttagatt cgatatctga agtcctttga
    12661 catcagcagc accgtatata ttctggtatt tatctaagat ctctttttca gataatgcac
    12721 ttttatttgc cgcctttttt gatgtcattt ctgcagcttt gttataatct cccttgttca
    12781 aagcttggac aaattgctca actgtctttt ctccagcttc tactgcttgg gtttcttgat
    12841 agtgctggta gtaaaaataa ccgccacccg cagctgctat taagatcact gcgccggcaa
    12901 tataagcgcc tgttcgattt ttgccgtgct tgtcacttct tttcattttt tcctccttct
    12961 cttgcttgtc attcctttat ttcactattt atacctgttt attctaacat atgtctttga
    13021 ggaaatccat accacaatcc agaagcacac tttctggaag gttcgtttgg atactaccaa
    13081 gtaatttccc aaccgctttg ggtagcttga atattgaagt tacgtctttc agctgctgag
    13141 agatagcttt gatcacttgc tgctgccttc tgatcctccc gaagtctcct tctttgtctt
    13201 cccgaaatcg agcatactgg agtaaagtat taccatccat cacttgctct cctttgttta
    13261 tatatacgcc atctaaatct agagattttt ctgcatcgat cttgactcca ccaggaaaca
    13321 gttcattaac gcaatcgata aaatcttgaa atgtgatact tgcataatag ggagcttcaa
    13381 atttgaaatt ttcctttaat gtttgattta ataaatcaac tcccccataa gagtaagctg
    13441 catttatctt attcattccg taacctggaa tatcgacata tgaatctctc atgatagaga
    13501 tcagttttgc ttgttgtgtt ttctggttgt attgtgcaac cagaagtgaa tccgagcgac
    13561 cgttttcgtc tttcgaacga gcatctgaac caatcaaagc cacatttagt gtggtaggga
    13621 cacttttcgg ctctaccttt ttcacagttg aactctgttt tgcagacgaa aatccatgga
    13681 taaactcaat cgaaaaatat ccaactacta atgtaagcag taacaaaata gataatatga
    13741 ttttttgaga tactttcatt gcgaattcct ctttctaaaa gaacctctcg cttgaaaaat
    13801 aaaagattgt actttatgat aattcaaact gagagtttct cagtttctct ttattgtttt
    13861 tcaggtcgat agagcggcaa ctcttcggcc tgtatctttg aagaaatatt caacgtgatt
    13921 ccgattccta aagaaaggat cagataactt gttcctccgt agctaacaaa tggcaatggc
    13981 acaccagtca ttggcatcag ccccgcgata cttcccacgt tcattatcgt ctgaacgaat
    14041 agcaaagttc cgaatcctaa aagaaacaat ccagcttgct gatttttaca gcgactgctt
    14101 aaacaaaaga ttctcatgca taatgagaac aataagaaaa gcacacacaa tgcaccgatt
    14161 agcccaagct cttctgtaat aatcgaaaaa ataaagtctg tttctgtctc tggaagatac
    14221 cctttttttg tgatactatt tcctaatccc cgcccaaata tgccaccatt atatagtgca
    14281 taaaacgaat gggtcatttg atagcccgca ccatgactat cgataaaaga atctcttaaa
    14341 gtcgcgatcc tttcataagc atgattaaac atctgtggaa gccaaccttt atttcctaaa
    14401 tataataccc cgcctgctgc tccaatcagc aatgcagaaa aagtaacgat tagatagatc
    14461 ccttttttaa atggaaccgc cgctgcccaa aatatgaccc acgcaatcga aaggatcatc
    14521 aatgctccag caatctttgg ctgaaataaa atcagaaaag ttatacctac tgtaatcagg
    14581 aatggttttt ttagatcttt tgggctacta tttccgtcac gaaaaaacca gcttaaataa
    14641 aaaatcaaaa aaagatttgc cagttcagaa ggctggaatt gaatgccaaa cagagagatc
    14701 caacgttgtg cgccgttgac agtgacaccg aatatcccta ttcttactaa tactaaaaag
    14761 aaaatcgata aggctaaacc gtatcctgct attttagggt gaagtaaata gtgtagtttg
    14821 actgaacgag ctaagaagat cacgctccaa ctcaaaaata tgaaaattag ttgacgcaat
    14881 aatagacttt ttgtattttc atcggcttgc aataaacgat aagaactggc actgtatact
    14941 tctaaaagac cgatcatgga caaagtcaaa taaggaccca aaatccacca atcgatcttt
    15001 tttctttttt tcaccgcatc tcctcctcac acagcaatcc acttgtcagg tgaggagcga
    15061 gtttgtctca tcaaataagt cagtcacaaa aatataaaga aaaggtcaga aaccttttcg
    15121 aacaagaatt ctgacctagc atcatctcat cgtccgagtt ttagcactat acaacgtaaa
    15181 gaaaacattc ttagctattg gaaaaagcct taatccgaca gtttctgttt tacccattgg
    15241 cgactttcga cgtttctggg caacagccta tgtttgtata ggagcctcac ctaacaaggt
    15301 tattactata ttctatttac gagttcatga tagacttttc ctaatgatta ggcaatggaa
    15361 tttgaaatga aatagtgaaa tttaacaatc tttaatattt tttagaaaaa aataacaaaa
    15421 aagttgaaag aatcccgttg caaattcttt cagttgcacc aggattcttt gataatttct
    15481 tagttttatg tgttttttta tatgtttttt tcacttttcg gaataaatat ttatttgtta
    15541 aagtaattaa acaatccgct aatcgtttga tctggcattt ctatttcctt accatctaaa
    15601 gtaacagtag taacgacaaa atggtcttgc aatggcggaa cttctaagta ctgcccttgc
    15661 agaaaatcat tcactgtttc atattctttt ttcatttctt ttgaatctgt cgatggttta
    15721 tagcttattt ttaacataac tagtgctcct ttttattcgg gacgtgcaat cgtgaaaatt
    15781 tctcctattg ctgcataatc atttcctgcc aaacggctta cagcattcaa ttttcttgga
    15841 tcgatccttc ctgtcttatc atcatagact gtttcattta aatgatattt ctgtacttct
    15901 agcaaaaata aatcagctgt tactttattt ttattccgta tctcgacatg ttcatataag
    15961 gatgtttcaa aacgaacttt tgcttctgtc aatgctggta ctgaaatgat tactgaatca
    16021 gttaaagtca agtcagtcag gtacagttcg ctttcctcat taggcagatt ggctgctgtt
    16081 ttatttactt cttctacgtt gtcctcatca aggatatgga taacagcttc tcttgtctca
    16141 agaatattct ttgctgtgtc ttttactgca tcttgttttc tctggatcgc caaggataag
    16201 atgggtcgat tcgaactgac gatattgaag taactgaagg gtgcgatatt cactatatct
    16261 tttgctgatt tagtcgttac tactgctact ggtctaggga tgactgaacc aatcagcaac
    16321 ttgtaatttt cacgttctgt caattctgaa ggactccgtt caaacatggt ttcgccttct
    16381 ttctataaaa ttcgttaaaa agcagatctt gaagacaaaa tgaaaagtaa tgcttttttc
    16441 atcttcttag aatctgcttt aaactatcta tcaatttttc ttttttgtca agaatctgtg
    16501 ccgtgcgtgt tttttagtta tttgctttac ttgcggcttc gacatatctt ttagtgatca
    16561 attgcggacg ggtgataata gatccaacta cgactgaaaa agcacctaat tcatatgctt
    16621 tctctacttg ttcaggagag tcagtatgtc cttccataat tactgggata tcgacttgtt
    16681 ctatgatttg attcaataaa tcaaaatcag gtgtctgcgt ctttcgtgaa tattctgtat
    16741 agcctgctaa agtcgtagac aaaagatcaa aaccatcttc ttgcgcttga agcgcttcct
    16801 caaaagtcga aatatctgcc atagctaacc gaccgtgttc gtgaatcatt tggacaagtt
    16861 cagcagttgt tttttctgtc ggacgtttac gagaagttgc atccagtgca atgatttctg
    16921 cttgcgtctc caaaagttct tccacttctt tttctgtcgc agtaataaac acttctgacc
    16981 catcgtaatc tcttttgata atccctatta ccggtaaatc aacgattcgc ttgatttcat
    17041 tgatatcttc tgtcgtattt gatcgaatac caactgctcc gccttctttt gcagccaaag
    17101 ccattctccc cataataaaa gaactgtgta gcggctcgtt ttccaaagct tgacaagaaa
    17161 cgacgactcc gcctttgatt tgttccagtg tatccacaat atctcctcca gtaatgttat
    17221 ataaaaatac agcttcatat aaaaatgtaa cccttttcta ttcaggagtc aataatgagc
    17281 tatttctttc agcagacctt tcttactaaa agaaaattac ttcaaattct tcgtatacga
    17341 ccagcttctc ttctgtaaat tctatgttca acgcttcttt ataagcatgt ttaaaaaact
    17401 cagcatggac tttccgccaa taagctaatg tccgatcccc ttctccttct ttccaagcat
    17461 gctctttcgt gacttcacta aaaggcaaga tggtcacatt tatatttttg atgatacaaa
    17521 ctggttcatt ttttccattc aggaccacgt ctaattgacc aacttttggt aaaggctcat
    17581 tctcttgttt atacagatcg taaccacttg atgtaccagt ctttatcttt tttacaacta
    17641 gttcgctcaa ctcgtctgcc attttttctg tatccccaaa tgaaaagaaa cctaattttt
    17701 ctgattgata actaggatat ttttcttgga actttttgac atattcttct acttgctgat
    17761 acataacatc cacccctttg ctttttataa tgaaaaaacc atccagatac tagtataaac
    17821 aattatctgg atggtgagca taatgaatta ttgaatattc aagacacgca gactatcttc
    17881 aacgatttgt tttggtgtta aatgataacg ttcataaagc tgccctacag gaacgctatc
    17941 cgtgaattct ttctctgcac caaaatttaa tacgttcatt gaagaatcac cataataacg
    18001 actgatattt tcgccaaatc ctcctgacaa actaccatct tctaatgtga tgactaattc
    18061 gtgtgtttct tttaatttat ctaatgtttc tacgtcattt tctgtgatcg ttcctggatt
    18121 gatcaacgtt gcttcgattc ctttttttct aaaagctttt actgtttctt ttcctaattc
    18181 aagaaaaccg cctaaagcta aaattgctac ttttcctcca ttatttactg tttgataggc
    18241 tggaatacta taatcatttg cgatagctgt accatgttct acaccatgtg taggaattcg
    18301 gattgctaca ggctgcgctg tttgttcgaa cgcccaatct agcatagaga tcatatcctc
    18361 ttttgttgct ggagcaagat atttgatgtt cgggatactt gataagtaag gaatgtcaaa
    18421 tgttccttgg tgtgttcggt cactagctga gatcgttccg ccaccgacta aaataatcac
    18481 tggattctcg ttgatcgcta aatcatgtga taattgatca tatgcccgtt gcaagaaagt
    18541 acttgattgg aagacgactg gacgtactcc ctgagaagcc aaagcagaag cgaaagtgat
    18601 agagtgctgt tcagcgatcc ccacgtcaaa ataatgatct ggatgttttt cttggaatgt
    18661 tttcagtcca aaggcgcctg gtatcgctgc attgactgcg gcaataggag ctgtacttgt
    18721 ttgcattttc ttatccaaat aatccatgat gatcatatta aatgtttctc cagaagttac
    18781 ttgctttgcc tccccggtgg acagatcaaa tggtacatgc caatgatatt tcattttatt
    18841 atcgattgcc ggctgataac cgcgaccttt ttccgtatgg acgtgaagta caatcggatg
    18901 atcaatatct tttacttctt caaataaacg gatcaccgtt tccagatcgt ttccattatc
    18961 cacgtaacga taatcaaatc ccattgcttt aaataggtta ttgctgcttt taccgttggt
    19021 ttgacgaagc tcagctaagt ttttatataa actgccatga ttctcatcaa tagacatctc
    19081 attatcgttt actaatacga ttagattgga ttgtaaaaca ccaccattgt tcaatccttc
    19141 aaaagctaag ccaccactta gggagccgtc accgataaat gctaccacat ttcctgattc
    19201 tttcttcaga tctctcgctt tagctaaacc tgttgctaag ctgatagatg tagatgtgtg
    19261 ccccactgta aaatagtcat gtttactttc atgttggttg gaatatccgc tcacttcacg
    19321 gtaatgtcct ttttcaaaac cattttttcg accagttaaa agcttatgac cataagactg
    19381 atgggaaaca tcccaaacga ttttatcctt tggtgaatca aatacataat gaaacgcaat
    19441 cgttaattct actacaccta gattcggacc aacgtgccca cctacatcgc tgtccttttg
    19501 caagattttt tggcgcatct cttctgccaa ctgtttcatt tcctcaacag ataatttttt
    19561 gacatctgcg gggctattga ttgcatccaa tacatgtaat actgtactca taatacattc
    19621 tctcctccgt actatcttct tttaatgaaa atagatttgt tacactttat ctattctact
    19681 acttagagtt gactctaagt caatagcatt ttaaaaaaat ttcagagaaa gcttgttata
    19741 taagtagatc ttcgcacgaa tcaggactca aataaaaacc ttctacagac aattaaaaag
    19801 ctttgtagaa ggtctcctaa ttaaggtctc atcaagaaat atgttttttt aataggtgaa
    19861 gttcctgagc aatatgatac aaatggatgt ttgcaccttt taatgagatt ggatagatct
    19921 ttgtcttttc agtttctaat ccattttgat agctagcagc atcgcacaaa accactcttt
    19981 ttttcgcaca tagacattca tcaggcaatt cttctaatga acgaaataaa ataggattta
    20041 tttcagcgat gccttcttcc gcatacaaat gaaattgggt tcgataaaaa aattgtttga
    20101 ctgaactttc tgaaacaaca aaaatttcta tttcctgtct ccccgcttgt tcactgttga
    20161 tcaaagaaag acttttgacg aattttcgaa tgaaattttc attccatctt agttggattt
    20221 gatttttctt tttccactct tccaatattt ctacaatttt tccgtacaga agttgttctt
    20281 tttctgatag aagatagatc ctttccggta aaaatacttg tccatctgcc cacaacgtac
    20341 ggacaaattg taaaaaggat tgcttgaaaa taaaattttc tgcatcgtcg atttcagcga
    20401 tactaataat ctgtaaaatc aattcattca gtgataggtc attgagaaca aaatttttat
    20461 aaacaacttc gaaatctttt cttaacagct ccatgttctc attggtataa tttctactgt
    20521 tgaacaaaga aaaaatataa tagacatctt cttcttgtaa gtctagaatt cctttttgct
    20581 cttccaatcc ttgacagact aattgaaata acggcttcgt ttctgcgctt ttcttatctt
    20641 cttctttcag tccaagggga aatgcctttc ttccgattgc gatctctatt cctcgtatga
    20701 taaaggactt ttcatcttcc gaatatcttc ttttaaaaaa atgagtctct acataatcaa
    20761 tcaactcgtt acaagcttct tctacatacg gatttttatt ttcatagcct tcccaattga
    20821 cataacgtac taaagctagc aagatgttcc gcgagtcaaa ttctgggata atgatttctt
    20881 tatttttcaa ataatcattt tctttaaaaa aatccaatac gatttttttg attgtatata
    20941 ctttgctcaa agaaataaat tcaagatcag ccagttctgt actcgtgaat ataccattaa
    21001 aataataact taaaaacttt aaaaaataag attgaccgta cagaaactgt gtgagctcag
    21061 aaagagcaat accatgtttg aatacgagtt tatacttatg cttctctctt acttgttctt
    21121 ttatacaata ttctagacac tcggctagac tttctagatc gtgaaggatc gttggtgtag
    21181 tcacccttag agaaatagct gtttgttcga tgtctatttc ttggaattca aataattgtt
    21241 cacacagata cacttttcta aatatatttc gctcgatgta attttccagc attcctgata
    21301 cacgcctcca gtttttctta tgattttatt taatcatatc tgtaaatcgt atttttgttt
    21361 cctttttatt tagaaaagcc ctttcaaaaa ccaataaaaa aagaaactgg gacaaaagtc
    21421 aaacaagctc acaatttcag cctcattgta ggtagctttg agccgtttgg acttttaccc
    21481 cagcctcttt caagaatggt tattcaaaga tgtcagcgtc tagttctttg actacatctt
    21541 tcaacaattg gataccagct gcgctgttac caaatggatc tagtgccggg ctgaagacac
    21601 ctatgccgta acgatttggc acagctgcca tcaacccgcc gcctacaccg ctttttgcag
    21661 gtacaccaac atggacggaa aactctcctg attcatcata caaaccagca gttgtcataa
    21721 ttgatttgac gattgtagca ctttcttcag taatgatttg tttgccattc caaggagcaa
    21781 ttcctttatt tgccagcaca gcagctaatt ttgctatgcc tgtagcggtg acattgaggg
    21841 agcattgtct gaaataagca tcgagtatct ccggtacgtc tccttcgatc atttgattac
    21901 ctttcatata gtaagcaaga gagcgattga tatcacctgt tcttgattca gaatgataga
    21961 tttcttcatt caatgtgata ttaggatcat cacagatctc tttcataaat gacaagattc
    22021 gctcaaattt ttcatctgca tctttaccgg caatcaacga ggttgttgca atcgctcctg
    22081 cattaacaaa aggattcatt ggatgtttgc gatgattgat ttccatattc atgatagaat
    22141 tgaaggcaaa accggttggt tccgtgttga ttgtttgaaa gactttttct attccgttgt
    22201 ctttgattgc taataacaat acgggaactt ttgacatgct ttcgatggca aagcgcacat
    22261 cggcgtctcc tccctcaatc agttgttttg tatccaaatc ataaatcgcc atacctaatt
    22321 gtttgggatc cacttcagct aatgctggaa tgtaatccgc cacttgtcct gtttgataat
    22381 agtggaaatt ttttccaatc aattgttcta attcttttac ttccatttga ataaccagct
    22441 ttcatttttt atttttctgc atgctggaat ttatgacggc atgcgtagat cgtaaatgga
    22501 ataactaaga cgataagata agaaacaatc agaatcgttt cataaatccc atgctgtcca
    22561 gctgcgatac tagcaggtgg aacaaatgag atacaaaaag caaaaagtga agtcaaaaga
    22621 cctaaacaag caacgattac ttttacaact tttccgcctg gaacctgata ggctctttca
    22681 agactgtcat ctttaaaaac cagcttcaaa tagcccgcga aaaacataaa gtacaacatc
    22741 aggtagatta caacagtcaa tgacatcgct gcaagaaatg aaagattatt ccccccgccg
    22801 cttaatgtaa agacggctgc ccagattgtt gcaacgatcc catttgcgat catcaaatga
    22861 gcaggaacac cgtgtgtgtt ttcttttgca aaatattcag gaagtaatcc ttcttctgct
    22921 gctacgtgaa gtgcttttga aggagaggtt acccaagaag caatttctcc gatgacacct
    22981 aaacaaacaa gaatcgcaag aattttcgca atccatgcaa gatttggatc aatgtgtctt
    23041 aataggaatt ctaacgattg aatcacccct gtattaagag acaaaccatg ttgaggtact
    23101 gtagtagcga cggtcaatcc gccaaaagta tctaaaatga ttgccagaat aaccaataca
    23161 aacattgcta atggataatc ctttttaggg ttttccattt catttgcatg actggcagat
    23221 gtttctacgc ccatatatgc taaaataaag gaaacaaaaa caaccagcgt aggcaatttc
    23281 gaaaaatcag gaatcaatgt tttcatagac agtatcgttt gtagtggagc gcctgaagcg
    23341 acataatgga tccctaagcc aaacaagact aatgctggca acaagatacc agctacaaaa
    23401 ccgatccgag caatcaatga agtgtatttg gttcctttta actgtgagag taaaatagcc
    23461 cagaaaataa tcaataggat tgccaatttc agatatttat tgttttgtat agcaccaata
    23521 cccgttgcat aggataatgc accagttaag aaatacagca tggtcaagta gccaactgtt
    23581 atctcaaacc attggaaaaa gattgcggca aaaccgaatc gttttcccaa tgtgcgactg
    23641 acccaagtat agacaccgcc tttttcccat cctttcaccg ttgccatctc tgctgcgcac
    23701 aatgccacag ggataaacca tagcaaaccg cctaataaca agaaaaatac taagctgaag
    23761 cctgaggtcg cgaatgtggg atattcatat aatgaaacga ccattgagat ggtcatagcc
    23821 aaaaagccaa agaacgttaa tttttttgca ggaattttcg ttttgctttg ttgcatgaat
    23881 cttcctcttt tctatttgaa tttgtttcaa ttgagtttgt tgccaaagaa gaaaaagtcc
    23941 aatccttctt cttgttcttc ttttgtagta gtgatcgttg taaagtgcat cgccgatttt
    24001 aaaatcactg ttgtcagcac ataggcatac acggcagtaa tgatgacacc aatcacttga
    24061 atccctaaga agtaccagct gcttggattc gttagtaaat ggctgttaga tgcaaagatc
    24121 cctatcagaa tcgtacctgt gaatccgccc atgccatgaa caccccacac atcaagtgca
    24181 tcatcccatt gtttcttttt acgaaaatca acagcagcat gacagacgat acccgcaatg
    24241 atcccaatca gcatggcact ttttgcttct acataaccgg cacaaggcgt aatggctgcc
    24301 aatccagcaa cggatcctgt tagtacgtcg acaaaatcaa agccatttcc ttttactttt
    24361 gcaatgatca gccaagtaat catcgcaaat gcaagagcga tgaatgttga accaaatgca
    24421 ttgactgctt gttcatctgc tcgaagtgca ccaccggaat taaaaccgaa ccagccaaac
    24481 caaagcagtc ctgtaccgat agcagctgcc ataagactgc tatgctgact tctttctcct
    24541 aaaggaatct ttctttgacc aagtgtgagg atacatgcta aagagccgaa tccagctgtc
    24601 gtgtggatga ctgctccacc agcgaaatct ctaaatccca tctgatccaa aaatccgcca
    24661 ccccatatcc agtggcaaac agggaaataa atcagcagat tccaaaaaat aacaagtata
    24721 atatagcctt ttaaattcaa tctccctgca aaagctcctg tcatcaaagg aacggtaatc
    24781 acgataaaca tcagttggta aagaaagaac attaaaaatg gaatcgttgc accatgaaac
    24841 atgttgggga agcttgtaac atgtctcatc aaaaagaaat ctgccgggtt tccaataaca
    24901 ccgccgatat cccgtccaaa ggccaatccg aatccaccaa atacccacat aatcgttgtt
    24961 atcccaacgg agagaaaaga ttgagacatg atcgttagta tattattctt ccgtgctaat
    25021 ccgccgtaaa aaaaagctag accaggagtc atcaggcaaa ccattgctgt acataaaatc
    25081 atgaatgctg tatctcctgt attgataggt aatgtattca tttttttcgc ctcctttcta
    25141 cgattacgag tataaaagaa ggtgtagttg tttttttctt ctgttttttt gaattaatac
    25201 ttgcatttat aaaagacaga caattaataa aagcttgatg tgttgtgctc tttttcgttt
    25261 ttcacacaaa taaagacaaa aaataaacgg tcttcttttt atagaaaacc gtttattttc
    25321 gtcttttttt atttttaaag atattttttc ataaggaata aagaatactt catataaaat
    25381 atataaaaaa gaaccgcagc gagtgtcgct acggttctca aatttgttct tcatttcaac
    25441 ataagttttt cttcaatttg cttataattt acgtgcaaag aatgaaacga ctaatactaa
    25501 aatgattgca ccgatcaatg atggaatgat agccattcct gcaattgttg gtccccaagt
    25561 tcctagtaaa gcttgtccaa ttgctgaacc tactaaacca gcaatgatgt ttgcgatgat
    25621 ccccattgat gatcctttat tcgtgattgc tcctgcaatt gctccaatga taccgccgac
    25681 gattaatgcc caaataaatc ccattttaaa ttcctcctaa ctgacttctt acttcttatt
    25741 gtacacgtgg ttcgttattt tctttgactt tttgtgcacc tttgcctaca gcagattttg
    25801 ctttatctgt ttggcgtgaa gcaaattgtc ctgtggattt tgctgcttct gttactttgt
    25861 cttgtactgt ttcttgatct ttttggtatt cttcttgtgt tttgatgtct gcaacattca
    25921 cattcacttc gataacatct aaatgtgtca tattgtttac ttcagtactg atcaattctt
    25981 tgatttggtt gtaaatatct tcgatgtctt taccgtattc tacgatcaca tctagatcca
    26041 cagctacttg tttcttacct acttctgtat tgatacctgc tgttacgtta tctgtgttga
    26101 ctaatttttc agccatattt gagaaaaatc cgccatctaa tgtcaataga ccatctacat
    26161 ctttcaatgc ataaccgatg attttttgga tgactttgtc ttcataagtc aattcaccat
    26221 tcatcatgcg gttttctcct ttttcatttg ccaaaactga tccatttgtt gtattcatgt
    26281 ttttgttttc catctttgtt tcctcctatg atttaaaaaa ttgatcaact attcctgact
    26341 gtttcaaata aaaaccgatt cctgcgccaa ttgctgtcat gattatgatc aacaatgtct
    26401 tgaaaaatcc aatcgttaac cataaaacag ctagcagtag tccaagaccc ccaaagataa
    26461 tagggagctt gtatgctgaa aatagttctt tcattatttg gccctcctat tccactcgag
    26521 agtgcttgta gtctcttgta tcttcttgtt cttcataacc gctgtatgtg actgccactt
    26581 tgaccgtttc ttttgttccg agtactttag ttacttcttc ttcgatatct tgcatcaata
    26641 cacctgtttt ccctactaat gaagaagttc gtttcaagtc tccgttgaca tggatatgga
    26701 ttcgattttt cgttgaacgt atccgtactt taggtgaatg aatgaactct tcttcgtgta
    26761 aatgggaacg taccaaccct tcgatcgctt ttttgtcgat tgttagtttg ccatcttccc
    26821 gtttcataac gaaagttcct cttgactttg gatagaaaag aacgactagc aagatgataa
    26881 tagcaagaac ggcaaatact acagctgccc agaaaatata ttgttgcatg tagtaattcg
    26941 ttaaagtaaa gaagcgaaat cgttccaatt gttcaggcat aatatacggc gcattgatca
    27001 acgcaatcgc tagaaatact gaaagtagca gtaaactcat gataacaccg attattttta
    27061 accccttatt cattttccac ctcctataaa cttattttta ccctgtaaat aactctaccg
    27121 acaatcacag tatagatagc gaacagttag catagcatca gtaaataaag cgctaccaaa
    27181 cattgctgga tcataacggc gttttattgc cagtctgata ctgaaataac aattgcttac
    27241 ttattctcta tttacttttg attgctggct atacttaaag gatattattc aagattaaaa
    27301 attgcaaaga ttatgctcag ttattattac aagattctat ttatttatta aattttttcg
    27361 gagaattaaa aaatacaata aataacttta gttactttaa tgcgttctta ctttattttt
    27421 atattatcat ttttcttatt cacttctttg gtttatacta ttcttataaa taaaaatgag
    27481 gtatgtccta tgatgaaaag attgtactat tcattgatca taacgattgg ctatttgatt
    27541 gtatcaaatc taggaaatat ggtatttggt atcagtaaag aattcagttg gactactaca
    27601 ttatgggaat ctctattctt tttcatcttc gtatttttgt tacaaaatta tcgaaaaaaa
    27661 taaacatgtg ccatctatct gttggcacat gttttaggat ttatggtact ttttattcgg
    27721 caatccattt tatttttcta cttcttctta atacctctaa cacttttgat ttataaacta
    27781 atccaatgca tctacttgcg cttattaagc tatttcagct agatagtacc accccatgcg
    27841 tcaatgcttg attttccttt tgtataacat tgtataagca ataaaggaca aataagtggt
    27901 gattacacca aaccattgaa taagaaaaat tctgatacgt aaataaacat gaacttgaaa
    27961 aaattaatga atcggttagc tactacgtag ttgtcttttt tctcatacag ttctgttaac
    28021 ttacacttct cttcgctaga taataaaaaa tcctccaact gtattgtcca atataatccc
    28081 aggcatctaa actagcatta tagaagagac acacatagaa tctaaaataa ctggttttaa
    28141 atgtttctta caatcaaaaa aagctgttaa atgcactcca caccttcata ctctatcatt
    28201 ttttataaac gatatatttt tattgttttt cgataaaaaa tagttgtttt acaataatta
    28261 ttctgctata ttttagctat caaagataaa agagggataa aaatgaaatt taaaacattg
    28321 ctattaaaat ttgtcgtttt aggtataagt gccgtattta ttttgttcgg cgtacttttt
    28381 tatactcaga tcgctacaag cgaaaaagga ttcaattttg attggcagac cacattattt
    28441 gtttctgtca tttactttgc ccttctccta gggatcgtta ttgcttattt cttattgaaa
    28501 ctattgaaaa aaatcgaaga taaccaagct ttttccactc aaagcttaaa catactaaaa
    28561 aaaatcgaac gaaccatttt gtttattggt ttcgcctttt ttggcttact tcctaaattt
    28621 atgaaagtag cggatttaga cgatgctcca ggtttgatgt tggtaggaat cgctgtcgta
    28681 ttcattcctt ttgcagtata cacattaaca agcgtgctgg aaaaactgct tgaacatgcg
    28741 attttactta agacagatca tgatttgaca gtgtgaggtg cgacatggca attatcgtta
    28801 atttagatgt agtattagct caaaaaaaaa tgagtgtgac tgaattgagt gagaaagtgg
    28861 gcattacgat ggcaaacatc tctattctaa aaaatggaaa agcaaaagcc atccgttttt
    28921 ccactctcga aaaaatctgc caagttttgg agtgtcaacc aggtgatttg ttgacctata
    28981 aaaatgactc gaatactata ccagaagaat aaaataaagt agaacaaaca gtaaataaaa
    29041 aataatctga atgatagatg tgtgagactt ttagaagtta tttacgtcta ttattcagat
    29101 tttttatttc gattgaatac cgttttatct ataaataaga tatgtactga ctcatacaaa
    29161 gggggccttt tcttctccat gtactatcac ttttcctgat acaactggtt tcctgttacg
    29221 ttgtagttgg atctgctttt agtataatac tttattcatt ccaccagact gatagcagtt
    29281 tgagcagtct aaataatcat gtagtattac aggccattct gtttcatacc ttgaagtgct
    29341 taaaacttca gctaatggcc cattgtgtta actcttactc cattcttttt tagtaagctc
    29401 ccatatatac tgtaaagcct tctatatatt atgtcatgtt gctttttaac atttactatg
    29461 tagttttgat aaaatgattt gaataacagt gatggacaat tcttttttat ttttaacatt
    29521 attttgataa taatgattat ttatttaaaa atacattaga taacatttgt tacgacacga
    29581 aaaagcttca caaacaaata tcgttatttt aaaatgaggg cgaactcaca caattaggag
    29641 gaatagtatg ccatttattg tttacttttt tctttcatta ctcgtgatct atattccatt
    29701 accaacattt ttcctgctcg tcaatcgtct agcagagcaa catgatacga ttgctgctct
    29761 tacgaaactt tgtttacttc tatttgtttg tctagattac tttacgagct attattggct
    29821 aaaacagaca agagtaaaaa agagatatta tttcttcctt gtctttttca atctctttga
    29881 gtttctgatc cattgctttt tacttcatca gtatcatacg atccatttgt ggatcatctg
    29941 ggttttcaaa gtggtcgtat tattttgtat gttcaccttt cctatctcaa gaaaatttag
    30001 ggaggcattg ttttattaaa gaagcttctc tgtatcaata aagacacatt taaggaaaaa
    30061 cttttaaaaa tctaattggg aatttgcccg cataaataag aggctgtgac aaaagtcttg
    30121 tcacagcctc ttattccgta taaacagtgt tcaaaagctt gcatcttcga taatatg
//
LOCUS       DACEUZ010000019        29904 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.019, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000019 DACEUZ010000000
VERSION     DACEUZ010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29904
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.019"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            22..399
                     /locus_tag="IXG44_000751"
     CDS             22..399
                     /locus_tag="IXG44_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1657489.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            1005..1619
                     /locus_tag="IXG44_000752"
     CDS             1005..1619
                     /locus_tag="IXG44_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ1657490.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            2392..4131
                     /gene="yfcC"
                     /locus_tag="IXG44_000753"
     CDS             2392..4131
                     /gene="yfcC"
                     /locus_tag="IXG44_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAQ1657491.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            4288..5472
                     /gene="iadA"
                     /locus_tag="IXG44_000754"
     CDS             4288..5472
                     /gene="iadA"
                     /locus_tag="IXG44_000754"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAQ1657492.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            5583..5786
                     /locus_tag="IXG44_000755"
     CDS             5583..5786
                     /locus_tag="IXG44_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAQ1657493.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(5887..6549)
                     /locus_tag="IXG44_000756"
     CDS             complement(5887..6549)
                     /locus_tag="IXG44_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1657494.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(6562..8064)
                     /locus_tag="IXG44_000757"
     CDS             complement(6562..8064)
                     /locus_tag="IXG44_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1657495.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            8612..10291
                     /locus_tag="IXG44_000758"
     CDS             8612..10291
                     /locus_tag="IXG44_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAQ1657496.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            10418..10896
                     /locus_tag="IXG44_000759"
                     /pseudo
     CDS             10418..10896
                     /locus_tag="IXG44_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
     gene            11115..11795
                     /locus_tag="IXG44_000760"
     CDS             11115..11795
                     /locus_tag="IXG44_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAQ1657497.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            11803..13134
                     /locus_tag="IXG44_000761"
     CDS             11803..13134
                     /locus_tag="IXG44_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ1657498.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            13149..14111
                     /locus_tag="IXG44_000762"
     CDS             13149..14111
                     /locus_tag="IXG44_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HAQ1657499.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            14222..14560
                     /locus_tag="IXG44_000763"
     CDS             14222..14560
                     /locus_tag="IXG44_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HAQ1657500.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            14639..15346
                     /locus_tag="IXG44_000764"
     CDS             14639..15346
                     /locus_tag="IXG44_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HAQ1657501.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(15449..16930)
                     /locus_tag="IXG44_000765"
     CDS             complement(15449..16930)
                     /locus_tag="IXG44_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ1657502.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
     gene            17239..17775
                     /locus_tag="IXG44_000766"
     CDS             17239..17775
                     /locus_tag="IXG44_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1657503.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            17853..19223
                     /locus_tag="IXG44_000767"
     CDS             17853..19223
                     /locus_tag="IXG44_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NOL1/NOP2/sun family putative RNA methylase"
                     /protein_id="HAQ1657504.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            19290..19625
                     /locus_tag="IXG44_000768"
     CDS             19290..19625
                     /locus_tag="IXG44_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1657505.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(19706..20563)
                     /locus_tag="IXG44_000769"
     CDS             complement(19706..20563)
                     /locus_tag="IXG44_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HAQ1657506.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            20670..21185
                     /gene="tadA"
                     /locus_tag="IXG44_000770"
     CDS             20670..21185
                     /gene="tadA"
                     /locus_tag="IXG44_000770"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HAQ1657507.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            21208..21292
                     /gene="ffs"
                     /locus_tag="IXG44_000771"
     ncRNA           21208..21292
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="IXG44_000771"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            21502..22887
                     /locus_tag="IXG44_000772"
     CDS             21502..22887
                     /locus_tag="IXG44_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HAQ1657508.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTDIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            22924..23601
                     /locus_tag="IXG44_000773"
     CDS             22924..23601
                     /locus_tag="IXG44_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HAQ1657509.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            23609..24373
                     /locus_tag="IXG44_000774"
     CDS             23609..24373
                     /locus_tag="IXG44_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HAQ1657510.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            24375..25034
                     /locus_tag="IXG44_000775"
     CDS             24375..25034
                     /locus_tag="IXG44_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HAQ1657511.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(25064..25573)
                     /locus_tag="IXG44_000776"
     CDS             complement(25064..25573)
                     /locus_tag="IXG44_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HAQ1657512.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(25685..26683)
                     /locus_tag="IXG44_000777"
     CDS             complement(25685..26683)
                     /locus_tag="IXG44_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ1657513.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            26884..27468
                     /locus_tag="IXG44_000778"
     CDS             26884..27468
                     /locus_tag="IXG44_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ1657514.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            complement(27545..28720)
                     /locus_tag="IXG44_000779"
     CDS             complement(27545..28720)
                     /locus_tag="IXG44_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ1657515.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            28802..29182
                     /locus_tag="IXG44_000780"
     CDS             28802..29182
                     /locus_tag="IXG44_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HAQ1657516.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            29256..29618
                     /locus_tag="IXG44_000781"
     CDS             29256..29618
                     /locus_tag="IXG44_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1657517.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
BASE COUNT         9731 a         5003 c         6197 g         8973 t
ORIGIN      
        1 aaaaaaaggt gtgtgttcac tatgggaaaa ttagtcgctg ttgcacagtc gctgcttgat
       61 atactcaagt ggctgatgga aggaagcaat tggatatttg tgttgccact attgcttatt
      121 agtctattta tcatgtttcg catggtcaga aaaaaagaaa cgtttctgat ttttatctgg
      181 tatgcagcat ccgttggggt tgctaccttg atggttgaat tggcgttagg tagttgggaa
      241 gcttgcgtat ttgctttatt ttttgctgta ctgtatctaa tcatttatat cccatatatg
      301 aaaagaaagt ctcggatagg cagaaaaggt agacatacaa ctactttttc tagtacagat
      361 tctctcgaac aaagtagaag aacgagaaga agacggtaat atttagtagg tatacgcaaa
      421 aaaagataaa ataatggata aactattgag atgagagatt tatttaattg gtggaaaaaa
      481 gcttttttca cttatataaa agaatctttt ttaaaggaat gcttgttgta tcgggataag
      541 ccattccttt ttttatgtta tttatatata tataaatcat accaatgttt ttttgtattt
      601 tcttgcttgc acttggataa gagattactt acaataaatt cagatagaaa cagctgaata
      661 aaaaaaggct ccctgaccct tagttggcgc taagaatcag ggaatgacca gtcacacaca
      721 tacaccctga ccgatcattt tgccatagtc aatgcagtgc atcgacctaa tttattgtac
      781 ctaatctagg gtgaaaattc tacccctatg tagatatttt ccatggtctg cacgttgttt
      841 tatttgctat gtcaaggtat tgggcgtatt cgttcaatgc cttttttgtg tttgaaacag
      901 caaagaccac atgtgttact gtctaactac tgttttttta tgtacttcct tagattaggc
      961 agctgtttct attcatgtca agtatagcta ggcgggagag ataaatgagt ttatgttatt
     1021 ttgactatta tcaccaattg gaagagaaat taaggataga agagccgctg atgcttcaag
     1081 ctggcacttg gatcgcgaaa caaattaatc aaggtggtag attgcagata tatgctagcc
     1141 gcacactaag tggtgtagca tttgaatttt gggatcaaac cccggatctt gtaccaagta
     1201 tattgattga aaatccagcc ggaggtgtct atgaaacgct agaaggtgca ggacaggcaa
     1261 ttattgatca gctttctgtc aaatcagaag acatttttct tcttctttcg aatgaaggaa
     1321 gaaacccggc aatcatcgaa ctagctcagt ggatcaaaga aaatggtcat ccattaatca
     1381 ttgttactgg atttgatttg tcacgaagta tcaaatcgca acatagcagt ggtttgcgat
     1441 tatttgaatt tgctgatttg gttttggaca accacgccaa tttacatgat gcagtcttga
     1501 ctttgccaaa tctagatcca gcgatctgcg gaacagcctc tcttgcaacg atctttttgc
     1561 tgcagcaagt tttgtatttt acagcaaaac agctagtcca ggacacaagt ggaaaatagt
     1621 tatccacatg ttgacaaagt tccttaaaaa aataatgttc cacataaaaa tggagcgttt
     1681 gaaaaaaaga aaacgattat taatgaggat taacaacgac tacttttttt gttttagtta
     1741 ttttaagatg gatcatcttt gattttaagc ttgttttgtt taaagtttgt tatttttata
     1801 agttgtttca cagttatgag aaatgagtta taagaatatg tagtgtcaga aaaataaaaa
     1861 gtagtggaaa aagagacaag aaagaatcca tatggattct ttcttgtctc ttaatacacg
     1921 ttgatgaaag agtcttcccc aaaactcttt acaaagtaat tatagtacat ttgttatcat
     1981 tattgcaatg aagtttacct gatataacaa aattgtccaa caggatacct ttttctaata
     2041 gaaataaaat acacaaaaat tgaaggatag ataatggata tttataagtg tttattattt
     2101 tttttgcaat gaaattcaat ttttcaatga ttgtaaaatg aaaaaaacag gacgagattt
     2161 tctctaatct aaaaataaaa aaagaatttt aaggaaaaac agagaaataa aatacttaat
     2221 ataaatggaa agagaaaaaa ataatcatac ataggagaaa aaaaacaaaa tagaaaaaag
     2281 tgattttcta ataaattatt catgtggaaa tgtcattttt actgtataat tcatttgatt
     2341 atccattttt tattcatcaa aattactgaa aaaagaaagg aagaaataaa tttgaagaaa
     2401 acaaattctt caggaaaacc gaaacgcgac ttcactaaat tatcgacacc ccatacgtat
     2461 gtcattattt ttggtgtagt catctttgca tggatactga cttttgtcgt tccagcagga
     2521 aagttcagca cgcaagatat tgaatacaaa gatgcaaatg gcgaaacaag tactcgtact
     2581 gttttacgtc aagattcctt ccgttatgct tacgagctgg ataagtcgta tgtgtttgat
     2641 caattagaag aactgcaaga tcatccagca gaacgtgaaa aactggatgt tccggaaaaa
     2701 ggattggaaa aagtcattgc tgacggagaa aaaaatctta cgcaggaaaa actagatgaa
     2761 atctccttga cagatgatgt gctttacgat cagtacggcg aaaatatcta tgatacttct
     2821 aaaaaattgc ataaaacagc caaaatatgg ggaacagatg attttggcgg ctttggtttc
     2881 ttaaattttg tttttgaagg actagtatct ggagataagt atggatctgc tgtagggatc
     2941 gctgcattga tccttgttgt tggaggtgct tttggtatta ttatgcgtac cggagcgatc
     3001 gacgcaggta tctatgcgtt tatcagtaaa actaagggac tggagcgttt agctttaccg
     3061 ctattattct ttgctttttc attcggtggc gctacattcg ggatggcaga agaggtaatt
     3121 ccattctcaa tggtgatggt tccatttgtc attgcgttag gatatgactc gatcgtagca
     3181 gtaaccgtga cttatgtcgc atctcaagtc ggaaatgcga cctcttggat gagcccgttt
     3241 agcgtagcgg tggctcaagg gattgctgga atccccgtgt tgtctggtgc gacattccga
     3301 ttgattatgt gggtagttgt tactgcatta gccgctggtt atatgatgat atatgcagaa
     3361 aaaatccgta aaaaaccaga aagttcgttg acttacaaat cagatgatta tttccgttct
     3421 catatcaaaa agacatctga tgaaaataaa aaattcatgc ttgggcataa attgatctta
     3481 ttagagatgt tagttgtcct tgtctggatc gtttggggcg tgactcaaaa aggctattac
     3541 attccagaaa tcgcttctca attctttgtg atgggattag ttgctggaat cattgctgtt
     3601 cttttcaaat tagatggaat gaaagtcaat gacatcgctt cttctttcca atctggtgca
     3661 gctgatttag ctggtacagc aatcgtagta ggtatggcca aagggattct tcttgtcttg
     3721 ggaggatctg atgcatctgt tccttcagct ttgaatacga ttttgcatgg gatcgggacg
     3781 gcgttgaccg gtgttccagc tgtcattggt gcttgggcga tgtacatctt ccaaagtttg
     3841 ttcaacttgg ttgttacatc aaattcagga caagcagctt tgactatgcc aatcatggct
     3901 ccactggcag atttagtagg tgtatccaga caagtagcag ttcttgctta tcagcttggt
     3961 tcaggtttta tggacgcttt tacacccgta tcagccagct tgattggtgt gctaggtgtt
     4021 gcacggatcg aatgggcaaa atgggcaaga ttccaaatca agatgcaagg attcctgttt
     4081 gttttaggaa ctattttcat catgattgcc attgcaatag gtctgcagta gtactggaga
     4141 cctgtaaata aaaaagtaat ccaaacagta gataaggact ccaagaaccg tcattttacg
     4201 gtaaaatcaa aggagtaaag atcctatcgt ttggattatt ttttaagatc ctatcatcag
     4261 ttatacttaa agaaagagga aaaatgtatg aaaatcattc gacaaattga agtgtttgca
     4321 ccagattact taggaaaaat ggatgtactg attgccggtg ggaagatttt ggctgtagaa
     4381 gaacagctag aaggcggcta cgaaggagtg gaagtagaag aactttcagg tgagggaaaa
     4441 gtactaaccc cgggtttcat tgactgtcat ttccatattt taggtggagg aggagaaggc
     4501 ggttatcaaa atcgaacgcc agaagtcaca ttgagccaat tgactacagc tggtgtaacg
     4561 acagttgtcg gttgtttggg aacagacgga gaaggtagag atatgactgc gttgatcagc
     4621 aaagcgaaag gtctggaagc agaaggaatc tccacttatg tatacgaagg ttcgtaccga
     4681 ttgccggtaa aaacagtaac agattctatc atcaaagact ttttgacaat tgataaaatt
     4741 atcggaatcg gagaaatcgc agtatccgat caccgttctt ctcagccaag ttttgaagag
     4801 ttcgctcgtg cagtagcaga tgctcgtgta ggaggcatgt tatccggaaa agctgggatc
     4861 atcaatgtcc acttaggcgg aggaaaaaga aagctagagc tattgacacg aatcgtagaa
     4921 gaaacagaaa ttccgatcac acaatttttg cctactcatg caaatcgcac accagaatta
     4981 tttgaagctt gcgtggcatt tgccaaacgt ggtggcacga ttgacttcac tgctagcgaa
     5041 gatcctgatt tttgggagaa gacagatggt gaagttcgct tcagcaaagc actgaaacga
     5101 ttgatcgagg aagatgtttc tttagataat ttcacgatga cctcagatgg acaaggaagc
     5161 ttgccttatt ttgatgaaaa caatcatttc ctagggttag gagtaggaag tgccaaggca
     5221 ttgctagtag ggatcaaaga agctgtgcaa aaagaaagta tccctttgga aatcgcctta
     5281 cgagctataa ccagtaatcc agcacgtata ttgaaattgg ataaaaaagg aaaaatcgaa
     5341 attggtgctg atgcagatct gtgtatcttg gataaagaaa cgcttgatat tgatacagtt
     5401 atcgctaaag gagaaataat ggtccaagaa aaagaagtca aagtatgggg gactttcgaa
     5461 aaaagttttt aatcaacagc tctaactatc ttttttagaa aacgagcgtt ctgtttgtct
     5521 gtatctgttg ttgatgttac cctgaaggta gatcaataaa caaactaaga ggaggaagca
     5581 caatggcaga tttaaaagga cgttctaaag atatgaaaga taaagtagtc ggtgaaggaa
     5641 aagaagcata cggcaaagca acagacgaca agagcaaaga aacagaaggg aaagcacaat
     5701 cactaggtgc tgacatcaaa gaaaaagctc gaaatttagg cgacgaagtc aaagacaagt
     5761 tttctcgaaa agacgaaaaa aaataaaaac cacttattac taattgaatc aagcaaataa
     5821 aaaatccgaa ccatatctgc gagacagata tggttcggat tttttatttg atcattatcc
     5881 tgttttttaa acttcgattt tttgaaattt tttatttttt agttcaaaga cttcatcaca
     5941 gctttttgct acttcaatct catgagtcac gatgataata cagcgatttt gttcatgggc
     6001 gatttcttgg aaaagtttga cgatatcttg tgaagttgtt tcatctagat ttccagtagg
     6061 ctcatccgct acgattagtt gatgttcaca gcagatagca cgaacgatag cgactcgctg
     6121 ctgctgacct cccgaaagtt tggtcacctg tttttttgct aatatttcat caattcctac
     6181 acgagccagt cgatctaaag caacttgtcg ataattctct tgcttggtct tagtgatttc
     6241 cattgctgct atgacgttgt ctagagccga taaataagga agcaagttgt aagcttggaa
     6301 aataatcgag acatcatttt tacgataact tcttaacccg attttgttca gtgaacgatc
     6361 ttgatagtat acctttcctt cttttggtgc atctagtcca gaaataagcg aaagaaaagt
     6421 cgtttttcca gaaccacttg ctcctaaaat agcgtacatt tttccagctt cgaaattcag
     6481 ttctacatct ttataaagcg catcttgcgg atcttgatac caaaatccca attgttctgt
     6541 ccgtaacatc aaattcctcc tttagtttaa taggattttt cttgggttta atcttaatat
     6601 ccctatagaa gctaaaaaga cagaaaacag actgattcca aatgctactc cggctaatag
     6661 tcccaattgt gcaggtttga cattgatttc caattcgctg acttgatcag aaacagcaaa
     6721 tggattattc gtggttcctt gttgaccatc gcgttgaccg ccttgttctt gtctgttacc
     6781 tggcatttgt ccaccgccag gagcttgctg gttttgtgtc gtggtttctg tctgttggtc
     6841 aagtagctgc tgtcccacgg cattcgcgac gacgtttccg ctaagcgaag caactcctaa
     6901 tgcaagaatc atacaaatag ccacttcggt aaacaattgg agaatgattt tacttctgga
     6961 ttctcctaaa gatagcaaga cgcctaactc atgccgacgc tcccgaatgg taatcataat
     7021 gatcagagta agtatgataa taccggctac agccactagt aaaacaacat ttttagaaaa
     7081 acttgccacg ttgtttaaag gttccaacat tgactgatac atggaatcat ttgattgtaa
     7141 gctaaatgta tctgtgtcga tcagcttctc agcttctttg agaaactcat ccattttatc
     7201 tggatcagat agagtgtaaa tagcagaatc tatcgtgtta tcgtcagatg tcccatttag
     7261 ttcatttgca aaagtgtaag aagtgtaaag tgtattcgac ggattcatga agttgaattt
     7321 catacccata ctgccgcttg tttcgctgga ttcataaatt cctttgatcg tcatttcata
     7381 agtggtatct tcatcttcag tactcgtgat tgtgaaagta tctccgactg ccaggtcatt
     7441 tgtttctgct agagtagaat caatgactac attgtttgtg cctttgtcgc tgtcatctat
     7501 tccttcacca tccgtgattt ttgctgttcc atcagaaaaa ttactatttt gtgcagttgc
     7561 tgacacacca ctgatttgaa agtctgcttg agtcatttgc ggattcccgc ccattcctcc
     7621 agcaggttca ccgcctgcat cagtagtttc tgaactgtct tcacttgaat cagaactagt
     7681 gatggcagtt attccatctt ttgccatagc tgaggcggaa ctttcaaagg aataactctt
     7741 tacattatct aattcagcta ttttttccgc gtccgttaag gaaactggtg taagagaaaa
     7801 actgcctgga tctggacgtt cttcagtggt agaaccgctt gaagaagttt gttcctgttt
     7861 tttaaatgaa gcttctcgat tggcacttaa agtcactgtt gctccaactt ctttttgtgc
     7921 ttgattcgct gcttcttctg cagcgcttcg aatcgtgagg ccagctagca caaaaataag
     7981 aatggctgtg aacacggcaa acaataacaa tgaacgtccc cattttactt ttgtactttt
     8041 ccatgcacgt ttcataaaat tcatattttg catccctcct agtagatgca tttgataata
     8101 gacaacctat cttaaagcta gcttaaatct tatgctgttt gcacaccatc aacatgaggt
     8161 tctgtatata taaaaagtca ctatagttat tgcaaaaaat gtggtaaaat gaaaaaagta
     8221 tgtttcaaac cgacgaagaa gaaatgaaag aggaatagca aaagaaatcc tagatgctac
     8281 agagggattt ttacgtgcat agttgttcta acaattgctg atactagcga tttagtttat
     8341 ttgttgtata ataatgggga acagctgaat acttatataa gtattctggc tatagcgttt
     8401 cgcaaaagat ccattttgtt aatgaagtat atccaaaata aaatgcccat ccaactgtca
     8461 gaacgcggat gtgctggtaa ttttcctgct gattctccat aagttgagaa atagtgattt
     8521 tgcatatgct tcattggaat ggctcatata tttagataaa gaagaaaatc gacatcttta
     8581 aacttggatg tcataagaag aggtgaagtt tgtgagtacc attaaaatta tcccattagg
     8641 cggcgttcgt gaaaatggaa aaaacatgta tgtcgcagaa gtgggcgagg atatttttat
     8701 tttagattgt gggctaaaat atcctgaaaa tgagttgttg gggatcgacg tcgtaatccc
     8761 tgattttacg tatttagaag aaaacattga tcgtgtagct ggtgtctttt taacacatgg
     8821 acatgcagat gcgatcgggg cacttcctta cttgttgtca aaaatttctg taccagtatt
     8881 cgggacagag ttgacgatcg agttagcaaa attgaatgtc agtcgtaatg aagcagccaa
     8941 aggatttaag gatttccatg tggtagatga gcatacagaa atagactttg gtgaaacagt
     9001 agtgagcttt ttccgtacca ctcatacgat accagattca gtggggatca atctgaaaac
     9061 aaaagaaggt agtatcgttt atacgggaga ttttaaattt gaccaaacgg ctatcccaat
     9121 gtatcaaaca gattatgcac gattggcgga aatcggcaaa gaaggagtct tagccttact
     9181 aagtgaatcg gcaaatgcag aaaatcctca gcctgtagct tctgaagccc aaatagcaga
     9241 tgaagtattt gatacgatca aatattggga agggcgtatc atcgtcgctt gtgtagcaag
     9301 taatttgcaa cgagtgcaac aagtattgga tgcagcagaa aaagcacaac gaaaagttgt
     9361 cttgactggt caagatttcg aacgaattat tcgcacagcg atgcgtttag aaaaacttca
     9421 attaccaagt gaagatctgc ttgttacgtt gaaagacatg aaaaactatg atccagaaga
     9481 actgatcatt ttagaaactg gccgaatggg tgaacccatc aagtctctgc aaaaaatggc
     9541 aaacggtaca caccgtaatg tcaagattga agaaggagat ttggtttata tcactactac
     9601 gccaagcatc gcgatggaaa ccatcgtggc gaaaacggaa gatatcattt atcgagctgg
     9661 tggaacagtg aagttgatct ctgagaatat gcgcgtttct ggccatgcca atcctaatga
     9721 cttgcaatta atgatcaatt tgatgaaacc aacatatttt gtgccagttc aaggagagta
     9781 tcgtgaatta gctgctcatg cagatttggc tcatgcagta gggatgccat acaaaaatat
     9841 ctatattact ggacgcggag acgtgctgga atataagaac aagcgtatga gtgttgcagg
     9901 aactacaagt gcagaaaatg taatgattga tggattaggc ataggtgata tcggaaacat
     9961 tgttttacgt gatcgaaaaa tcttgtcaga agacgggatt ttcgtagcgg ttgtcactat
    10021 cagtcggcga gaaaagaaaa tcatttcaaa acctcaaatt acttcacggg gattcgttta
    10081 cgtaaaagct agccgtgatc tgatgaaaga aagcagcaat atgattgaaa caatcgttga
    10141 gaaacattta gaaagcaatg attttgagtg gagcaaatta aagcaagaca tccgagatca
    10201 attaggccgt tacttatttg aacaaacaaa acgccgccca gtgatcttgc cagtcattat
    10261 ggaagctact caacgtcgaa ataaaaaata aacaaaaaga gtaagatgaa cctctctata
    10321 gagtgctcgt cttgctcttt ttttgcgaaa aacaaataat ttttgaatac agcatccctc
    10381 gctttataat aaacgataga ataaagggag tgaatcaatg atgagtcaaa caaaagtcga
    10441 acgttattta gaagaacaaa agatcagcta tcaaccactt gattttagtc atgtggtaga
    10501 agaatctttc acagaagaac tacaaaaaag agggattgat ccagctttga tatgtaaaac
    10561 tctcgtagtg aaaggaaaca aaactggggt gatcatcgca ttgattcctt tgaacgatca
    10621 cttagattac aaaaaaacga gacaagtgac gaaagatcgt aaagttggtt ttcctggaat
    10681 ggattttgta ttaatgatac aggatatccc cacggagcaa acacccctat tggaataaaa
    10741 ctcgcacatc ctgattattg ttttttagca gacagttctt taaaagaaaa agaagaagta
    10801 attgtgtcaa gcggtgaaat cgggagaagc cttcggataa aagtagccga tttagaaaag
    10861 atcgtccatc ctatttacgc tgatttaatt agttaaggga cgagaagaga agctaagcag
    10921 agcagagacc tcaaacttga gattatatct gcttggtact tggtgcatcc tgtattatct
    10981 gttacaattg ctttgtacat actgtactat caatccaatg aaaatatatt tagcagctat
    11041 attttttcat tgatgtgcaa gtgatgaaat cgtttttaat aaaaaagata cagagaaaac
    11101 gcgaggtgta aaatatgaaa attacgtatc atggacattc ctgtttaaca attgaaatgg
    11161 aaaatggcca acgcgtgatg attgacccgt ttattacagg aaatccattg acagacttaa
    11221 ctgcagaaga agtaaagaca gattggttgc tgattactca tggtcatagt gaccacatag
    11281 gagatatgat tcctattgca aaagaaaatc aagccacaat tatcagtatt gttgagattg
    11341 ctgattatgc gaacagcaga ggagtagatt cattcggtat gaatatcggt ggaaaacatg
    11401 cgtttccatt cggcgtggta aaattcgtac atgcacagca tagttcaagc tatgaagtag
    11461 acggtatcgt tcagtatatg ggcgagccta gcggaattat tattcaagca gaaggaaaaa
    11521 cgatttatca tgcaggggat actgcttatt tcagtgatct aggattgctt gcagaagaat
    11581 tcgatattga tgtcgctttc ttgccgattg gcgataacta tacgatgggg ccagaagatg
    11641 cagcaaaggc tgcgcaaaca attcgtgcca agaaagtagt tccaatccat tacaatactt
    11701 tccctgtcat tgaacaagat cccgaatctt ttgtagagtt actggaagat agtgaaggaa
    11761 aaataatgag cgtctcggaa acgctggaag tgtaggcaga aaatggcgac aaaacatgat
    11821 ttgattttag aatatattga aagtttacct gttggcaatc gaatttctgt aagaagcatc
    11881 gcaaaagaat tgaatgtcag tgaaggaaca gcgtaccgtg caatcaaaga tgcagaaaat
    11941 gtcggactag tttcaacaat tcaacgagta gggaccatcc gtatcgaacg caaattaaaa
    12001 aaacatattg aacgtttgac atttggcgag gtcgtcagga tcattgaagg ggacgtttta
    12061 ggcggggcgg caggattaga caaggtcttg aataaattcg tcatcggcgc gatgactaaa
    12121 catgccatgg aacgctatat tacgccagga tcactaatga tcgtcggcaa ccgggcggaa
    12181 gtccaaaaat tagcattgga agatggcgcc gcagtattga tcacaggagg attcaatacg
    12241 actgctgaga ttgcaaagct agctgatgat cttgaaatgc cgatcttacg cacctcttat
    12301 gatacattta cagtcgcaac catgatcaac cgagcgttaa gcgaccaatt gatcaaaaaa
    12361 gatatcatgc ttgtcagtga tatctatatg ccagctgaaa agactcatta tttgcagcag
    12421 acagatacga tttctgatta tcagcgtttg tcagaagaaa cgcagcattc gcgttttcca
    12481 gtcgtcaacc gccatcatcg tttgatgggg attgttacgg caaaagatgt tttagggaaa
    12541 tcgccaaacc agatcatcga tcgggtgatg acgaaagaac caatcagtgt aaagaaaaca
    12601 atgagtattg cctctgttag ccatcaaatg atctgggatg ggctagaagt catgccggtc
    12661 gtttccgatg acttgacatt ggaaggcttg attacccggc aagatgtaat gaaagcgatg
    12721 cagcttgtcc agcgacaacc gcagattgca gatacgattt cggatcagat atctggaaat
    12781 atccatacga ttgatactga ccgtgaagga aatcgtttgg aaaaccctaa gttcaaattc
    12841 gtagtagcac cacaaatggt caatggcgtt ggaaccattt cttttggcgt acttagcgaa
    12901 atcatttctg atgtcgcaca aaaaacaatg gtgatgaacc aaaaacgaaa tatcttgatt
    12961 gaacaagtga acctacatta tttgcggctg atccaattgg aaagcgaatt agacattcgt
    13021 ccacgtgtat tggaaatcgg cagacgttcc gctaaactgg atatcgaagt gtttattgaa
    13081 aacaccatcg tttccaaagc aatcgttgtt tgtcaagtaa tggaacgttc ttagaaagca
    13141 ggagtgaaat gacagtacag caagaaatag tagatacaat aaaaaaatac gataaaatca
    13201 ttatccacag gcatttgcgc cctgatcctg atgcattagg atcacaagta ggattagccg
    13261 aactattacg ggaaagtttc ccagaaaaag aaatacgtca agtaggtgaa acgattgaag
    13321 ggcttcgctt tttagcagaa atgcaagaag ttgaagatgt cttctaccaa ggagcgctag
    13381 tcatcgtgac tgatacagct aattcaccca gaatcagtga tcaaagatat cagttggcag
    13441 aaaaactgat caaaatcgac catcatccca atgatgaccc ttatggagat cttgtgtggg
    13501 taaatacaaa agcaagtagc tgtagtgaga tgattgcaga atttgctttg atgtttcctg
    13561 aatctttaaa gatgaacgtt aaggctgcac ggttacttta tgcaggaatc gtcggagata
    13621 cagggcgctt tttgtatccc tccacgacgt cccgtacatt agagattgct tcgatattga
    13681 gaaattatcc atttgatgca agtgctttga acagagagat tgaacaaatg ccgatgaaag
    13741 tcgctaaact ttccggttat ctttatcaaa atatccaggt ggatgaaaat ggtgcaggaa
    13801 gaataatcct tccgcaagaa attcttgata aatatggaat cgatgattca gagacagctt
    13861 cgatcgtttc acttcctgga gtgattgacg aagtgctggc ttgggggatt tttgttcagc
    13921 agccttcagg gtattaccgc gtacgtttac gttctaaggg gccgatcatc aatgaattgg
    13981 ctaaacgcca tcatggagga ggccatccat tagcaagtgg tgcgaatgca aaagatttag
    14041 cagaagtaga ggaaatctat aaagagatcc aagatatatg ccaatcgtat aagagcagaa
    14101 aaacagaatg atacttcgtt cgaaaacgtg ccttttgtag taaaaatcac aaatgtatct
    14161 tttcaagatc ttctttttcc gttacaatag tgctcactaa agattttaaa ggagaaagaa
    14221 gatgacatta ccaaattgcc cagaatgtgg ctcagagtac acatatgaag atcgaggctt
    14281 gttgatttgc ccagaatgcg ggaacgaatg gaacaaagaa gaacaagaaa ctgtcataga
    14341 agacggatta gtggtattag atgcaaatgg aaaccgtctg ttagagggcg atagcgtgac
    14401 agtgattaaa gacttgaaag taaaaggtgc gtcaggtgcc attaaacaag gaacaaaagt
    14461 caagaacatc cgtttagtag aaggcgatca taatatcgat tgcaaggtag aaggatttgg
    14521 accgatgaaa ctgaaatcgg aatttgtgaa aaagaattaa agaatattca aggtcttttc
    14581 acattctttt cctactacta atgataaaat aaagtaaacg aaaaggagtg aaacgaaaat
    14641 gtatggttat ggaatgtatg gttttttaga tccgacattt cttttggtca ttcttggatt
    14701 agttatttcc ggaattgcgt cagcgtacgt caatagtact ttccgaaaat acgatcaagt
    14761 gagaagcaaa aacaatgtga ctggtacaca agcagcacaa tatatattac aaagtcaaca
    14821 gataaataat gtcggtgttc aacaaattgc cggagatctc acagataact acaattcagg
    14881 taataagatg ttgagtttgt ctcaagcaac tgcacagtct acttcagttg ctgcgatagg
    14941 tgtagcagct catgaatgcg gacatgcggt acaagatgct tcgggttatg tcccattgaa
    15001 gattcgtgca gcacttgttc cagcagctaa ctttggttcg atgatttcgt tccccttgat
    15061 tatggtaggt gtattgctta gctggaactc tacgttaatc aatatcggga ttttcgcttt
    15121 ttctttagca ttacttttcc aattggtgac attaccagtc gaatttaacg cgtctcggcg
    15181 agcaatccaa attttgagtg aaggcggact actgacggaa gaagaagtac cgatggcaag
    15241 acacgtatta tttgcagcag cactgactta tgttgcagca gcgctttcga cattcctgca
    15301 gctgttgcgc ttgatcttat tgttcggcgg caaccgaaga gattaaaata aagaagttgt
    15361 gaaatcagcg ctttgatctg agctaatacc gagagttagc tgattgaata ccatttattt
    15421 ggaataagag gatgtgacaa gaattttgtc acatcctctt tgttttgttc tcataacctt
    15481 ttttcatata cagttcatga accgctttta ttaacttgcc aaataacact gtattgtgcg
    15541 ttgtatccag atagaaagtc tccgtttcat ctgaaatttt ctcatgctga tctgtaatga
    15601 tcaaatcagc atgttctgaa tatgcggtaa ttttaacagc atctttgcga aataactgct
    15661 gcaaatggct ttgaagatga taatttcctg taaactgttt ggttatctgc aaatggatga
    15721 gtaactgctc tttcgtttct gtttgtagta agtgttggat caattgacta aggtattgac
    15781 tgatttggtt tctttcccca ttttttgaaa aaattgtatc gattttttct cttaatttct
    15841 tttcctgtat aaaagaacta gaatgattgg acgtggcttc tggaaagcaa agtgtagaaa
    15901 aactactgct ggattcaccc aacagttcga aaaaaacaac tgccaacaca agaaaataaa
    15961 aataaattgg tattttattt tctaaatggt tgagaggaaa ctgttgagtt aagtaagtca
    16021 tcaattgacc gacttgtcga caaataaggt ttttgcttgt ggaaaataaa aaaccaatct
    16081 cctgtttttg tcgaaaagag atttgatttg gaaaaaaaca atagaaaaaa taattaaagt
    16141 aggctctctc tgtttctgtc tcttcacaag ggcgactcac tgaaaaagaa gtaaagtatt
    16201 ctgaataatc atgtgtatgc tgcaataagt ccatcaattc agtgatttca gaagaagttt
    16261 ttggaaaata tgcctgttgt ttgtttcgta atatgaataa agttaacagc aagtagatat
    16321 ttttttcttg ttttgaaatt ttttctattt gctcatctgt actgttcatt tttagtgcat
    16381 ttaattcaag tgaggaaaat ggccaatcgt tacctccaaa tgtatctgaa agaaaaaagt
    16441 aaagatagag acggatagat aactcatttc cttcaagagt acagcaattt tttacagata
    16501 ggatccgtaa atggaatttt tttaaggaat gattgagctg ttttgtcagt ttttcgagat
    16561 aggagggcga tatataaagt tgttcaagta aatatttttt tggtacagca gtttgtggat
    16621 aattgattag taacatcaaa tagttaaaac gattggattc ttttaggtag cataatttta
    16681 acttagaaaa aacagctaac tttttagttt gatcggtttc tttggtttgg atctctcctt
    16741 tatgattaga ataaataaat tcatcaatca ataaaaattg cttgagtgat acatttattt
    16801 ttcggatcag ttctttgatt gtccgttttg gaatatgtaa gtcattggat aattcttgta
    16861 aagaacaatt atttttttga atgaccattt gtagaacaag taactctttt tttcttgctt
    16921 caattagcat aaatacctcc tttttattct gtttttgtat atagttagac aaaaactgtg
    16981 agtggaaagt catttcagtc tgtgttattc tcacagagtg tcgccttctt ttttatataa
    17041 cgttatttaa tttttttgtt accttgttta ttctattgtt tatttcctag ggtattttgt
    17101 caagatattc ttgtttttaa ggcggaaaaa ttgattttta atggatataa ctaaatatgt
    17161 tttgataatt atcaataata tttttagaat tatttatgtt atttatttat gggtagatga
    17221 aaagagacgg aggtaagaat gatggggaga gtaacaaatt atagtaaaga atatttgctg
    17281 ttcaaaagta tggtatacgt ggaagaatat ggaatgaagt caattactgc aagagagtta
    17341 gcagagttct gcggttgttc tacatacccg atttatacac atttcaaatc aatcagcggg
    17401 ttaaaagaga agatattaga agaaatcact gtttattttg aaaactactt agctgaatgc
    17461 agttccgatg atgttttaag cattgtctac ttattgaaag attttttttc tatccatgaa
    17521 agtattcgtg aaatcattgc aaaatctgat ttagacatgg attcgctatt taagcggcca
    17581 tttttctcat atgtcaaagg tcacacgaag atcaaagaag aaaagtcgct tgaattggta
    17641 tggcttaatg cattgggaag tttgcgctca gatgattctt ctcaagattt tcttgaaatg
    17701 atgaacgctg tttgggataa aaacgaggtg acggatgaat acttggaaaa tatcttaaag
    17761 gaacagtcac cttaggcagc aacatgaaaa agtagatcag ccacctttat ttttcgtaaa
    17821 aaagattgta aaatgtctaa cgaaacattg gaatgaagga ggaagcgaca acgttaccac
    17881 agcaatttat caaaaaatat cgtcttcttt taggcgaaga agcatccgat tttttttctg
    17941 cgcttgaaca aggaagcgtg aaaaaaggat ttcggtggaa tccactaaaa ccggcaggat
    18001 tggacatggt ccaaacttac catagcgaag aattacaacc cgctccctac agcaatgagg
    18061 gatttcttgg cactgtcaat ggaaagtctt tcctccatca agctgggtat gaatatagcc
    18121 aagaaccaag tgcaatgatc gttggaacag ctgctgcagc aaaacccggt gaaaaagttt
    18181 tggatttgtg tgcggctccc ggtggtaaat caacccaatt agctgctcaa atgaagggga
    18241 aagggttatt agtgacgaat gagatctttc cgaaacgtgc gaagatttta tcagaaaata
    18301 tagaacgatg gggagtttcg aacgcaatag tgacaaatca tgcacccgca gaacttgtcc
    18361 ctcatttttc gggctttttt gatcggatcg tggttgatgc tccttgttca ggagaaggaa
    18421 tgttccgaaa agatccaaat gcaataaaag agtggacaga agaatctccg ctatactgtc
    18481 aaaaacgtca gcaagagatt ttgtcttcag caataaaaat gctaaaaaat aaaggacaac
    18541 tgatttattc gacttgtacg tttgcaccag aagaaaatga agagatcatt agctggcttg
    18601 tagaaaatta tccagtaacg attgaggaga ttcctttgac tcaatccgtt agttcaggac
    18661 gttctgaatg gggaagcgta gctggtttgg aaaaaacaat acgtatatgg cctcataaag
    18721 atcagggtga aggacatttt gttgcaaagc tgacttttca tggacaaaat cagatgcata
    18781 aagaaaagaa gacacgaaaa aaaagtaaag tgcaaatgac aaaagagcag gaaaaactct
    18841 ggacggagtt ttcaaatgat tttcattatg aagctactgg aagactttta gtgttcaacg
    18901 accatctttg ggaagttcct gagttagccc cttctctcga tggtttaaaa gtcgtacgta
    18961 caggtttgca tttaggagat ttcaaaaaga atcgatttga acctagctat gcattagcgc
    19021 ttgctacaaa aaagatagag aatatacctt gcttgccgat cactcaaaag gaatggcaat
    19081 cttacacagc aggagaaaca tttcaacgtg atgggaatca aggatgggtt ttgctagtat
    19141 tagataagat acctgtcgga tttgggaaac aagtaaaagg aacagtaaag aactttttcc
    19201 ctaaaggatt gagatttcac tgagtggatt agttcgttta tttttttgtc tatgctatga
    19261 tggagataga taaaaaggag ctgatactta tgaaaacatc aacaaaagta acaatcggat
    19321 taagcgtagc agccgctgcc agtgtagcga cagcagtcgt tgtatcggga aaagtaatgg
    19381 aaaagatcca tcatatgact actcggacga aagtaaaaaa attcgtctat gacaaatttg
    19441 atggaaatga aaaattacta gaaatcgtag atggtctttc agatagtgac ttggattctt
    19501 tgatgggaat gttagcaaaa atcaaatcag gaaaaaagaa aatctctgtt tatggtgatt
    19561 caatcaaaga ttcaaccgaa gatgtaaaag accggctaat gcattttgtt gaaaaaatga
    19621 tgtaagagaa actagctttt gaacactatt cattcgaaat aaggggttgt gacaaaatta
    19681 ttgtcacaac ccctttaatc tatctttaat tatgttcatg aagcaataaa agatagcaaa
    19741 acagtaaatc gttggctttt tttagattga aaccagactg ttcttgaaat ttttcgattc
    19801 gatattttag agtattacga tgaagaaaca gtgtttttgc agcagagctg acattgccta
    19861 attcattcca taattcttga atgatcactt ccatttcatt ttgtttgatc agttctatgt
    19921 aacttttgat caacttacta tcttttacag tatcttttgt atagtaatgt aaagccacat
    19981 cttgcataga aaaagtacgg ctgtgagtat aaagattgtt ttgctctgtt aaaaaaattt
    20041 ttctttcttc agcaaacaaa gggacgaggt cggtgccagg tgaatggaaa gaaccaacga
    20101 atacagacgt tgtcgtatca aaatcagcat ctaaagatag aaaaataccg ttgatttcag
    20161 tagcatcaag ataatcacgg tttatttgtt cgactaagag cgcatctgtg tctgtataaa
    20221 aaaagaaatc agcagaatag cgaaataatt ccaaaatagt atcttgccat tcttttttta
    20281 agaattctcc ttttgattcg acgtgaagct ggatcaaacg aaagcttttt tcttgaacac
    20341 aagaagcatc tttaaaaaga tagtgatacc atgggtgttt tttttgatcg ttgattacag
    20401 ggaataacgc tttcaaaagg ctggtttctt gttgagataa tgaatcttgt ttgatccaga
    20461 taaaagcatc atcaattgca aaagaaagga tatccgcttc tgatgctgga gagttttttt
    20521 tctgtgcttg tgggtaaaga gcaagtaatt tatccatttt catcttgttc acctcttgta
    20581 tttattttac catagaaaaa aaagttagtt acagcctttt tatcatatgc taaagtaaaa
    20641 caagaaaaaa caaaggcagg gaagggtaat tggtaaatac aaatcttgat gcagtagaaa
    20701 aagaaaaatg gatgcgcctt gcattggcag aagcaaagaa agcagaaatg cttcatgaag
    20761 ttccgattgg agcagtggtt gtattggatg gaaaagtgat tggaagagga tataacctta
    20821 gagaaaccac gcaagatgct actacacatg cagaaatgct tgcaatcaag gaagcctgtg
    20881 aaaaagtggg aagctggcgc ttggaagatg ctgcactttt tgttacctta gagccttgtc
    20941 ctatgtgcag cggcgcgatg attttgtctc gtgtagcaga agtttatttc ggcgcgtatg
    21001 atccaaaagg cggaacagcc ggtaccttga tgaatctttt ggaggacgag cggtttaacc
    21061 accaagcata tgtagaaggt ggcattttag aagacgaatg tgggatgatt ttgactgact
    21121 ttttccgaaa gctccgccag cgaaaaaaga aattaaaaaa agactggtca acgaatgaaa
    21181 aataaggtat actattcttt gccgaaaggc taggacaatg gtgggcttgt gaagcgtgtc
    21241 aggtctggaa ggaagcagca ctaagcaggt tccgccatgt gtctgattat aatgaaacga
    21301 caacagactt tcgggtctgt tttttttatt tttatagtac tgtaaaaatg aatgttcctg
    21361 attttttagc cagactgatt tatcgaatat tgtacttata caatttgctt caaggcagga
    21421 aaaactctga aatagagctt gtttcaatgg aagaaaaagc tgcctatggt atgataaaga
    21481 taaagagagg cgagagggta catggaaaaa atagtgattt tacatacgaa tgatctgcat
    21541 tctcatttag aaaattggcc aaggatcaga aggtttttgg atcaacgtaa acgagaaaac
    21601 gaaaagaaag aaaaaacgac aacgattgca gtagatctag gtgattttgt ggatcgttgg
    21661 catccattgt cagaagcaac gaatggacag gaaaatatct tgctgatgaa tgaagttggt
    21721 tatgatgcag cgacgatagg aaacaatgaa ggcgtaggaa attccaaaga agagttgaat
    21781 catttatacg atcatgctga gtttgatatc atcctggata acttattcga taaaaatacg
    21841 ctccagccac cggcatggac gaaaccatat aaaatcatta caacgaaaca ccagacgaaa
    21901 atcggactaa ttgcatttac tgctccattt ccgcttactt ataatccgaa tggctgggac
    21961 atacggaatc cttatgatat tttgccagaa ttagtcgaag atttacgttc acaagtggat
    22021 gttttagttt tgatgagtca tctcgggatt caggatgatt tccaaattgc aaaagaaata
    22081 ccagcaattg atgttatttt aggatcgcac acccatcatt tattcagaga aggtcacgta
    22141 atcaatgatg tccaaatagc agccgctgga aagtacgggc agtatgtcgg tgaagtccat
    22201 ttaaccatcg atgaagagaa aaaaatcctt aagcattcag ccaaagccat ccctaccgaa
    22261 acaatgacag cttttacaga agatgaacaa gaaataaata gttatctaga acgtgggcat
    22321 gaattattaa aagaaaaaga agtagcagat ttgccatttt ctctgagtct agatatcttc
    22381 aatgagcatt cgtttatcca agtagccttt gaggcggtga aagaacgagg aaagacggaa
    22441 gcagcaatat taaacagtgg tctgttttta actgatattc caaaagggcg ggtcaatcag
    22501 gatcagcttc acactgcgtt gccgcatccg atgcacttgt taaatgtcac attgggtgga
    22561 aatgatctga tccgcttagt tttagaaata gaaaaaaatc gtaacttttt acgtaactat
    22621 cctatgaagg gaatgggatt tcgcggtaag atttttgggc agatggtcta taatggaatc
    22681 acatatgatt ctattaacca tcaagtcttt tggaagaaca agccaatcga tctgaataaa
    22741 aattatactt ttactaccgt agatcatttg atgtttgttc cattttttcc aacgattgag
    22801 attgctggaa aaaatgaatt tttgttccct gaatttatcc gaagtgtggt aggagattac
    22861 ttgaaagctc actacccaat caaataaaaa caaggtataa taaaaaagat aggtggtgaa
    22921 cgcatgtctg caaaacaaaa tgaacagcag aacgttgagc ataaaacaga aattgctaag
    22981 aaaaaatcat cagctgaaca ggaaacaaca gtcaccgaag aactgacagc tgctttggaa
    23041 gaaatcgttg aggagcctaa agcagaaaaa gtcaaaggcg agctagtgac actgttagat
    23101 gacagtaatt ttttgatagg tgaaagacat tatcggcttg tgtccgatta tcgtgaagga
    23161 tttaatgcag aaaagcttgg agaacgttac agtgatgtac tagcccgata tgattatatc
    23221 gtaggagatt ggggttatga acaactacgc ctaaaaggat ttttccaagc ggataatcga
    23281 cgggcgcatc cagatcagcg aatcgattcc ttggaagact atctttacga atattgcaat
    23341 tttggttgtg cgtattttgt catcgagaga atcggcggta aaaaagaaaa gacaactcag
    23401 cgccgaaaaa agaaaaaaaa ccataatcgt gcgtatatcg atgagaaaaa aggaccagtc
    23461 gcatctaatc gcaaaaaacc ggttattaaa aatcgaaaaa cagagtcagc aaaaacctct
    23521 gaaaaagtaa aaaaacaaga acaacctaaa caagtagaaa aaacaaaaac agcatttacg
    23581 atcagaaaaa gggaagagta gtggatgaat ggactataaa ggatatttaa ttgatttaga
    23641 cggaacgatt tatcgtggga cacaacccat accggccggt aaacgatttg tggaagaatt
    23701 gcaaaaaaga aaattacctt tcttgtttgt taccaataac acgactaagt caccagaaac
    23761 agtagctaat cgtctagcag atgagtttga cattcatgta gctccagaga cagtctacac
    23821 agctactttg gcaacaatcg attttatgaa agcagatgga aaagggaaaa aagtctatgt
    23881 tatcggagaa gcaggattga ttgacttgat tttagctgcc ggatttacat gggaagaaga
    23941 ggctcctgac tatgtagtgg taggcttaga taattatctg acctatgaaa aagtagttaa
    24001 agcaacactt gctattcaaa aaggtgctac tttcattgga acgaacccag ataagaatat
    24061 acctactgaa cgcggccttc ttcctggagc agggtcagtc atctcttttg tagagacagc
    24121 aacccaaacg ccgccgatct acatcggaaa accagaagcc atcattatgg ataaagccgt
    24181 tgaagtatta gggctgcaaa aagaagaagt aatcatggtt ggagataact atgaaacaga
    24241 tatccaagct gggatacgca ataatataga tactttgctc gttttatcag gttttacaaa
    24301 agaagaagat gtacctgggt tgcctatccc agctacctat gtgaaacaat cactggatga
    24361 atggagcttc taatatgaaa aattcgagat ggcagtggat ggaatatgct ggattgttca
    24421 gcctatttct gacactgatc agtctggcag ttggtgtgac gatcaacttt cgaccactat
    24481 acgtatttga tatcggacat ttgcaaattt tggattatac ttctttagat caagagacgt
    24541 tactgaaaaa ttttgatcac ctaatgaatt atctcaataa tcccttcaaa accatcctca
    24601 gcttgccaga tttcccagtt tctgctagcg gagcccatca cttttatgaa gtgaaaattt
    24661 tgtttttagt ggattatgct gtgtttttca tcactttgat cccgagtatt ttgtttatca
    24721 aatatttgca aaagaatgat cggctttggc ggttgattcg tccgtttcaa atcggcatgt
    24781 tactgccagt tgttttcggt tttttcatga tgataggatt tgatcgcttt tttatccttt
    24841 ttcatgaaac gtttttcaac aacgatgact ggttattcga tcccgttact gatccaatca
    24901 tcaatgtact tccagaagaa tttttcatgc atagctttat cttatttttt gtattattag
    24961 aattattttt tgctgttttt cttttcttag gaaaaaatag cttgaaacaa acaaaaaaga
    25021 aagaactcgt ctaaatagac gttttctttc tttttttgtt gaattattga gtaaggcgac
    25081 ttcttttttc tggttctttg tgtgctaatc gactggcagc agcagcggcg atcgctccga
    25141 cgatatcatc taagaaggta tggacatgaa cgccatcgtg gctgtttagt tcttttaaaa
    25201 taccaggttt caccttgtcg atataaccat aattcgtaaa gccgatcgta ccgtaaacat
    25261 tcacgattga caaggctagg atctcatcga taccgtaaag gccttcgtca tcttcgataa
    25321 tatgttgcaa cggactcatc aattttttct tttctgctaa tatatctaat tggattccag
    25381 tgataatggc gttatgtact tcacgctttg ttagcacgct gttgacactt tcagtacaat
    25441 ccgccaaagt caaatcagaa atatagtctt tttgtaaaaa catgacaagt tgtgcgatat
    25501 cttctacagt tacgccacgt tccttcaata gttctcttgc cttttcttct aaagtctcag
    25561 ttttgacgac catattattg ctcccccttt attttttctt tctctatttc atgttaacgg
    25621 aacatgaaaa aaatgcaaga aactgctttc gggtttaact tgaaatgcaa agagatataa
    25681 aaaatcaact aggacgaaga tttttatcgt ataagcttgt gctgtgacca ggctgagttc
    25741 gttcctttgg attaatatag tgtaaagcgt tgttcactgc agtaggtgct tcaccgagcc
    25801 cagtagcaat cagtttcact tttccttcat aggtacagat gtcaccagct gcataaacgc
    25861 ctggaataga agtagacata tctgatttta cagtaatggc atttctggta ctatcaagtc
    25921 cccaagaaga aagatgttct aaattagaag agaatccata attgacgatc agagaatcaa
    25981 tcatcaaatc aatcgtctcg tcacttttca ctttttttaa ccggatatct gtcagttccc
    26041 cattattgcc tgataatcca tcgatgagat agggagtaag caagttgaca gatgaagatt
    26101 tcaagcgtga tacactatgt tcgtgcgcac gaaattccgg gcgacgatga atcaagtaaa
    26161 cttcgcttgc aataggctct aacatcaacg tccaatcgat agcagaatca ccacctccag
    26221 caatagcgac tttttttcca gcatacttca tcaaatcatt cacgaaataa tccaatccgt
    26281 ggttttcaaa tgattcagca ttatctaaat tcaattttct tggctggaat gaaccatttc
    26341 ctaaagctaa aatgactgct ttggaataat ggatgccttt atttgtagta atctcgataa
    26401 cttcgtcttc acgagtaagc gagagaactt cttcttttaa atggaatgta tggttaaaag
    26461 tagttaattg tttttctaat tgatcgatta attcacttgc ttttattgct ggatacccag
    26521 gaatatcgta aatatatttt tcgggataca aagttgctaa ttgaccacca agttgaggta
    26581 gactgtcaat gatttttgtt ttagcttggc gcattcctgc gtaaaaagcg gcaaacatcc
    26641 ctacaggacc tgcaccgatg attgtaatgt catagatttc cataaaaatt cctccctttt
    26701 cacactatag cagaatttaa ttgagaatgc ttctcaaaaa tgtttgaatt cgaaatcgaa
    26761 acggtaagtt atagaatgta tctagtgatg aaaaataaaa gaaaacaaat ctagtatttt
    26821 tacgagaggt taggtattat gttattatta ttatgaagta tgcaaatatt aggaggaaac
    26881 aatatggcat tcccacaact agatttaaaa agtgaaaaag gaccaaaagc tgttatcaaa
    26941 acaaaccgtg gtgacattac tgttcaatta ttccctgaac tagcaccgaa gacggttcaa
    27001 aatttcattg aactttccaa aaaaggttat tatgatggcg tgatttttca ccgcgttatt
    27061 cctgatttca tgatccaagg cggcgatcca actggaacag gaatgggtgg cgaaagtatt
    27121 tatggcgaaa gttttgaaga tgaattcagt cgtgaactgt tcaaccttcg cggagcttta
    27181 tctatggcaa attcaggtcc aaacacaaac ggaagccaat tctttattgt aaataacaca
    27241 aatgtgccag ctaatatgct tggacaacta gaaggtgctg gattcccatc agaaatcatt
    27301 gaagcgtata aaggtggcgg aactccttgg ctagatttcc gacatacagt ctttggacat
    27361 gtactagaag ggatggacac agtagatgaa atcgcaaatg tacaacgtgg tccacaagac
    27421 cgacctgtac atgatgtagt gattgaaaca attgaaattt cagaatagtt tggtatggta
    27481 cggagtaatt atgaaaaagg acgaaaccta gtaaggtctc gtccttttat tttgaataaa
    27541 tgatttaatc gattgtctct aaagcatact tgattcgatt aagtccttcc agtaaagttt
    27601 cttctggaca agcaatattc aagcgcatat gcccatgacc ggaaggacca aacgtgatgc
    27661 caggatttaa tacgactttt cctttccgaa caagagtgtt ctccaattct tggtcgtctg
    27721 aaatataggc ggtaaaatct aaccacatta agtaagttcc ttcagggttc attattctca
    27781 ctttcggaag atactgttca aaaaactgat taactgtttg aacatttttt tctagataag
    27841 gaatcagttg gcttaaccat tctccacccg tctcataagc cgcttgggtt ccaatcagtc
    27901 caaaagtgtt gatttcgtgc tgttggttca tcactaaatg tttttcaaaa tcagctttca
    27961 tctctggatt tttgataaat accattgaat ttttgattgc agctagattg aaagtcttgg
    28021 ttgcggaagt aaaagcaact agtagatcat ctaaattagg atcaatcgtt agcatggaag
    28081 taaatgtatg atcgaataaa gttaaatctt gatggatctc gtcactaaac agaatcactt
    28141 gatgttttaa acacaattcg cttaactgtt tcaattcttc tttgctccaa acacgccctc
    28201 caggattgtg aggattgcat aaaatcattg cttttacatt gttttcttcg atttttttct
    28261 ccatgtcaga caagtccatg atgaaatgat tatttttttc tagcaaagag cttcgaacga
    28321 tgtgacgttg atttgtttct ataatagagg aaaatggagg ataaactggg tcatgaatca
    28381 aaatagaatc gcctggtttt gtaaaagttt gtactgctgc agctaaactg gttaacacac
    28441 cacttgtaaa aacgatattt tctttcgtca actccacagc atggtgttgg cgttcccaat
    28501 gaatgatagc ctgatatagt ttatcagaca gagtggcata accaaagatt ccatgtttga
    28561 tgtattcact aaacgcttta gctaccccac cgggagacaa gaagtccata tctgctaccc
    28621 acaaaggaag taagtcatcc atttgatatg tctcagcaat tgcgtcccat tttacacttt
    28681 ctgtatcttt tcttgaaatc tgtttatcaa attcgatcat cttaagacct ccagtcattt
    28741 tcgtcgcttc tttttttgag ctatttacag tataatgata aatgcgaaga gagggaagta
    28801 gatgacttat aaaattggac aaataattga aggaaaagtg acagggatac agccttatgg
    28861 cgcattcgtt tctcttgata acgagacgca aggacttatt catgtttcag aagtacaatc
    28921 cggttatacg aaaaatatcc attcattgtt aaaagtcgga cagccagtaa cagttcagat
    28981 catcgacatt gatgaatatt ccaaaaaaat cagtttatct ttaagaacgt tagaaaaaac
    29041 aaatccagct gttccatatc gcagaaaaag gtatttcacg aataaaaatc gtaagatcgg
    29101 atttaccact attgcggagc agttacctat ttggatagaa gaagcattat ctgaactaga
    29161 acggaaagaa aataagaaat aaaaagtgta ttttttctga aaatgtgcta gaatttttat
    29221 gtaagccata gaacaagttg atcgaggtgg aaattttggc aagcaaaaga gttgctggta
    29281 caattatgtt gcatttggag gatggtacta aaaaattttt ggtccattca attgatgaca
    29341 agttggaatt tgcgttagcg gaactttctg aaggaaagac tggtttagcg aatatactta
    29401 atttcttaaa agaaatagtt catatcgatg ttagcaagat tagtttgatg gaattgacaa
    29461 atacacatat taaagaggaa aatgttccgt tgtttgtctt tgaaactgaa caaaagaatc
    29521 aaagagaaga attaggcgaa ggatatattt gggaagaacc aggtcaaatg cgctctgttc
    29581 ttggaactta cgaagtagag ggagtgcctt tcttctaaaa tacagattaa gcgaacaaag
    29641 aagtttgaat ttttttcaaa cttctttttt tattgtttgg tccaaaaaat acaatgaaat
    29701 gttctgctaa agatagtttt ttagtgcatt ttattttcaa aatatgttga aaacggttgt
    29761 ataacgaata ataataattg atcgaaaaat catttcacaa agtaatgctt aatttatagt
    29821 tgaccaagga agaaatacat gataaattta acagcgttga ttctgaagtg gatgttatat
    29881 cacatttcta aaaaatttaa aaaa
//
LOCUS       DACEUZ010000020        29110 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.020, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000020 DACEUZ010000000
VERSION     DACEUZ010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29110
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.020"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(<1..1031)
                     /locus_tag="IXG44_000782"
     CDS             complement(<1..1031)
                     /locus_tag="IXG44_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1657518.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMVALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTV"
     gene            complement(1113..1802)
                     /locus_tag="IXG44_000783"
     CDS             complement(1113..1802)
                     /locus_tag="IXG44_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAQ1657519.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(1908..2276)
                     /gene="rplL"
                     /locus_tag="IXG44_000784"
     CDS             complement(1908..2276)
                     /gene="rplL"
                     /locus_tag="IXG44_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HAQ1657520.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(2329..2832)
                     /locus_tag="IXG44_000785"
     CDS             complement(2329..2832)
                     /locus_tag="IXG44_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HAQ1657521.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     gene            complement(3242..3931)
                     /gene="rplA"
                     /locus_tag="IXG44_000786"
     CDS             complement(3242..3931)
                     /gene="rplA"
                     /locus_tag="IXG44_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HAQ1657522.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(4035..4457)
                     /gene="rplK"
                     /locus_tag="IXG44_000787"
     CDS             complement(4035..4457)
                     /gene="rplK"
                     /locus_tag="IXG44_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HAQ1657523.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            4800..5468
                     /gene="sdaAB"
                     /locus_tag="IXG44_000788"
     CDS             4800..5468
                     /gene="sdaAB"
                     /locus_tag="IXG44_000788"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit beta"
                     /protein_id="HAQ1657524.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            5481..6353
                     /gene="sdaAA"
                     /locus_tag="IXG44_000789"
     CDS             5481..6353
                     /gene="sdaAA"
                     /locus_tag="IXG44_000789"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HAQ1657525.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(6444..7463)
                     /locus_tag="IXG44_000790"
     CDS             complement(6444..7463)
                     /locus_tag="IXG44_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAQ1657526.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(7636..8523)
                     /locus_tag="IXG44_000791"
     CDS             complement(7636..8523)
                     /locus_tag="IXG44_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ1657527.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(8600..9145)
                     /gene="nusG"
                     /locus_tag="IXG44_000792"
     CDS             complement(8600..9145)
                     /gene="nusG"
                     /locus_tag="IXG44_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HAQ1657528.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(9246..9416)
                     /gene="secE"
                     /locus_tag="IXG44_000793"
     CDS             complement(9246..9416)
                     /gene="secE"
                     /locus_tag="IXG44_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HAQ1657529.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(9440..9592)
                     /gene="rpmG"
                     /locus_tag="IXG44_000794"
     CDS             complement(9440..9592)
                     /gene="rpmG"
                     /locus_tag="IXG44_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAQ1657530.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            9754..10398
                     /locus_tag="IXG44_000795"
     CDS             9754..10398
                     /locus_tag="IXG44_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ1657531.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(10454..10957)
                     /locus_tag="IXG44_000796"
     CDS             complement(10454..10957)
                     /locus_tag="IXG44_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAQ1657532.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(10970..11016)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            11554..12519
                     /locus_tag="IXG44_000797"
     CDS             11554..12519
                     /locus_tag="IXG44_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAQ1657533.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(12618..13790)
                     /locus_tag="IXG44_000798"
     CDS             complement(12618..13790)
                     /locus_tag="IXG44_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1657534.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(13777..14922)
                     /locus_tag="IXG44_000799"
     CDS             complement(13777..14922)
                     /locus_tag="IXG44_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1657535.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(14936..15868)
                     /locus_tag="IXG44_000800"
     CDS             complement(14936..15868)
                     /locus_tag="IXG44_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1657536.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            15985..17064
                     /locus_tag="IXG44_000801"
     CDS             15985..17064
                     /locus_tag="IXG44_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HAQ1657537.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGISPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            17061..17690
                     /locus_tag="IXG44_000802"
     CDS             17061..17690
                     /locus_tag="IXG44_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1657538.1"
                     /translation="MITVVIIDDDPFVTKSLQTILESTKEIRVLGIGHCAKDALVLYE
                     THRPDVLLTDIRMPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYL
                     IKQNLQAIIPSVKSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKE
                     VAAGKNNKEIADALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(17800..18027)
                     /locus_tag="IXG44_000803"
     CDS             complement(17800..18027)
                     /locus_tag="IXG44_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ1657539.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(18130..20718)
                     /locus_tag="IXG44_000804"
     CDS             complement(18130..20718)
                     /locus_tag="IXG44_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HAQ1657540.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(20738..21397)
                     /locus_tag="IXG44_000805"
     CDS             complement(20738..21397)
                     /locus_tag="IXG44_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ1657541.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(21446..21949)
                     /gene="trmL"
                     /locus_tag="IXG44_000806"
     CDS             complement(21446..21949)
                     /gene="trmL"
                     /locus_tag="IXG44_000806"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HAQ1657542.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(22170..23036)
                     /locus_tag="IXG44_000807"
     CDS             complement(22170..23036)
                     /locus_tag="IXG44_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAQ1657543.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            23145..23786
                     /locus_tag="IXG44_000808"
     CDS             23145..23786
                     /locus_tag="IXG44_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HAQ1657544.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(23848..25209)
                     /gene="mgtE"
                     /locus_tag="IXG44_000809"
     CDS             complement(23848..25209)
                     /gene="mgtE"
                     /locus_tag="IXG44_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HAQ1657545.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(25227..26126)
                     /locus_tag="IXG44_000810"
     CDS             complement(25227..26126)
                     /locus_tag="IXG44_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAQ1657546.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(26128..26925)
                     /locus_tag="IXG44_000811"
     CDS             complement(26128..26925)
                     /locus_tag="IXG44_000811"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HAQ1657547.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(26903..27607)
                     /locus_tag="IXG44_000812"
     CDS             complement(26903..27607)
                     /locus_tag="IXG44_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HAQ1657548.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            27738..28313
                     /locus_tag="IXG44_000813"
     CDS             27738..28313
                     /locus_tag="IXG44_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HAQ1657549.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            28431..29108
                     /locus_tag="IXG44_000814"
     CDS             28431..29108
                     /locus_tag="IXG44_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAQ1657550.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
BASE COUNT         8566 a         6168 c         5232 g         9144 t
ORIGIN      
        1 aatacagtat tatttcggtt gcttgctgct tcacctagcc cttgtactcg tgtttgattg
       61 gcgacttgac caaattgatt cccgacgtct aataaaataa tagctaaaag caaaatggcc
      121 aaatggcttc cgccaaccaa taaagcaata aagcttattg tttgcataac aagtcccata
      181 tacacgattt ttctttcaga ataagaatct gccagtcgtc ccacgatagg tgcagcaact
      241 gctccagaca aaccaaaaat cgctaatatc cctgcttcga atgttcccca attgtaggcg
      301 ggactgctga tataaaaaat caacgtagac caaaagatcg aaaatgttcc aaacatgaaa
      361 aatccactga ctgccgcttc tcttaataga cgttgagaag caactaaatg cggcaaactt
      421 ttaagtgaat caaggtatga aacagctgta ctactttgct ggacagaacg cggcattttc
      481 cagtaaagca gaagtgtaag aattccgact gcacataaag caaataaata gatcatccgc
      541 caagaaaaaa gactggcaac aataccagat accgtccgag aaagcaaaat ccccacgagt
      601 aaaccgctaa gcaatgtacc catgaccttc cctcttgctt ttggtcccgc tagtaccgca
      661 ccgtaaggaa tgatgatttg cggcacgatc gaaagtaatc ctattaaaaa agaagcgcat
      721 gcaaataacg taaacgaagg cgcaaaaaaa gcggccaaca gagagacggc agataacgct
      781 accattcgaa tgatcaattt tggacgatcc accacgtccc ctaaaggaac taaaaacagt
      841 aaccctaacg catagcctaa ttgggtcaac atcgtcaata tcccgactgc acttgtgcta
      901 acagataaac tagctgctac ttttgttcca atcggctgga tgtaatacat attggcaaca
      961 acaacgccac aagtgattga taatagtaaa ataagcgaag ttgttaaagg tgttttttca
     1021 ttactcttca ttgatttccc ccatttccta tcttagttta caacttatgg aaaagattgg
     1081 aaatagaaaa atacaactat tcgccacact tattatttgt gcttttgttt tccttgtttt
     1141 ttcttgcgat ctttataaat gaagacaact aaccctacta gcacagtaac acttgcaacc
     1201 aacgtgactg cagttttgtt atatccgtga aacagagctt ctccaccaca cgcatacaac
     1261 acagacgaag ggatgactcc aatcacgatc gacaatatga tttgctgtag aggcaatttc
     1321 atcgcatctg cagcaaaatt gaccatagaa gatggaataa ccggtatcat atagcctaac
     1381 attacaccta tttttggatg tttcatttgt cggattgctt gcacccaatg attcgtttcg
     1441 gttttcttat ctaaaaattt taagcgatgc ataaggaaaa ttgcagtgat gttaccaaga
     1501 gcattaccca aaatgttgat gatcgcacct gtcagtggtc caaagctcaa accagcaatg
     1561 actccaacta ctgaagtagg aatacctgga acagcacaca tcaccgctgt caatacgatc
     1621 aagagtatcg atgcaaaagt cccatgcgaa cgaatatgtg cgagtagctc tgccttactc
     1681 gctctcggat ttaaaaataa ttggatgtct gtctgatatt ctaaatacaa ccgatagccc
     1741 actaccagta tcagaatgat tcccaacgag atcaacatca atcggattat tttctttttc
     1801 atatttcctc ctgaaaaaaa tctatattca ttaaataata gtttaacata aaaacaaaaa
     1861 agcaccgaaa tccaacatag gatctcagtg cttttaaata gtaaaaatta tttaacagtt
     1921 acagatgcgc caacttcttc aagtttagct ttcaattctt cagcttcttc tttagatacg
     1981 ccttctttaa ctggtgcagg agcgccatca actacagctt tagcttcttt caagcctaaa
     2041 ccagttgctt cacgaactgc tttgataact ttaacttttt ggtctccagc tgcagttaat
     2101 tctacagtga attctgtttg ttcttcagca gcagcagcgc caccagcagc agctacagct
     2161 acaggagcag cagcagatac gccaaattct tcttcaatag ctttaactag gtcgttaagt
     2221 tctaggatag ttgcttcttt taactcagca acgatgtttt caatgttcaa tgccattata
     2281 atttcctccg tttaattgtt cttttttatt taatgcttta tgcggcaatt aagctgcatc
     2341 tccatcttct ttgtctgcca ctgctttgac agcgtaagcc acgttgcgga ctggcgcttg
     2401 tagtacagat agcaacatag atagaagtcc ttcgcgattt ggcaattttg ccaatgctgt
     2461 gatttcttcc aatgaagaga ctttaccttc gatgataccg cctttgattt ctaatgcttt
     2521 tgcttcttta gcaaattcgt ccatgatttt cgctggtgcg actacatctt cgttactgaa
     2581 tgcaacagct gtaggtccag tgaatacttc gtccatgcct tcaaggccag cttttttagc
     2641 tgcacgtgaa agaatagaat ttttgataac tttcatttct acgttagcat cacgaagttg
     2701 tttacgtaaa cgtgttactt catcaactgt taatccgcgg tagtcaacga ctactactga
     2761 tgcagctgct gtaaattttt cgcttacttc atcaacgatt gctgcttttt ttgcgataat
     2821 tgcttcactc atttttgagt ttcacctcct gaattttgat ggaagagttt tttgcactga
     2881 aaaggcaagt cacttttata gttactttgc tcaaccagta ttattcatca cctattcact
     2941 tcgttcatag tgattctact cgctttgctc gaaaggaaac tcctttcgct ttgctcaacc
     3001 agtattgttc atcatctctt cacttcgttc atagtgattc acaataaaaa actctatgcc
     3061 accgcagaca tagagggact attgaatata ttcaataaac gtcctcggta ggaaattaag
     3121 gcttgatgcc acctactgtc ttcggtacaa gttaattatt aacctcaact agcttagcat
     3181 gctaaactag ttgagtcaag aattattttt cagttggatc aatcaacatt ctaatattta
     3241 attagaaaga agcttggtca acatggatac caggtccaaa tgttgttgtg atcgttacat
     3301 ttttcatgta ttgaccttta gctgctgatg gtttagcttt tagcaataca tcatggattg
     3361 ctgcaaagtt ttcaactaat ttttcatcgt caaatgaaac ttttccgatt ggtacatgga
     3421 tatttccagc tttgtccaca cggtaagtta ctttaccagc tttcacttcg tttactgctt
     3481 ttgttacgtc cattgtaact gtaccagttt tagggtttgg cattaagcct tttggtccta
     3541 atactcgtcc tagacgacct acagtagcca tcatatcagg tgttgcaaca actacatcaa
     3601 agtcaaacca tccgttttgg attttttgaa ccatgtcgtc gtcacctacg aaatctgcac
     3661 cagctgcttc agcttctttt gctttgtctc ctttagcaaa aactaaaaca gtttgtgttt
     3721 taccagttcc gtttggtaat acaacagcac cacggatttg ttggtctgct tttttagggt
     3781 ctacgttcaa acggtatgca acttcaactg ttgcatcaaa ttttgcaatg tttgtttctt
     3841 ttgctaaagc tactgcttcg gcaacaggat aaactttact tgaatcaact ttttttaatg
     3901 catcttgcat ttttttgctc tttttagcca ttttggttcc tccttgattg tggttataac
     3961 ggttgtacct cccacgtgtt ctatatcttt caatatagag ccaactgaga agctgctgtc
     4021 ttagggtgta tagattattc tacagtgatc cccatgcttc gtgcagttcc ttcaaccatg
     4081 cgcattgctg cttcaacgtt agctgcgttt aggtcttcca tttttaattc agcgatttct
     4141 cttacttgat cgctagaaac tttcgcaact ttatttttgt ttggttcgcc tgaacctttt
     4201 tcgattttag ctgctttttt caatagaaca gcagctggcg gtgtttttgt aacgaatgtg
     4261 aatgaacggt cttcgtatac agaaataaca accggaatga tcaaacctgc ttgatcagct
     4321 gtacgagcgt tgaattcttt tgtgaatccc atgatattga tacccgcttg acctagtgca
     4381 ggacctactg gtggagctgg agttgcttta cctgcaggaa tttgcaattt aacgattttt
     4441 tctactttct ttgccacgag acatacctcc ttaagtccgt gatgtggtta attggagatg
     4501 catatttctc ctcccacgtt ttatctgatc tgaaaaaatc cagacaaata atgtgcaaaa
     4561 caatgcacac tggtacagta tagcaaattt atttgttttt tcaagatttt tttctgctat
     4621 ttttacattt tgcttgaata aacgctaaat ttattctaaa aaaacaacaa tttttcgttc
     4681 tcatttcttc ttttatgtta cacttgcgct agcatggttc aacagatgaa agcaaacaat
     4741 aacacggttt tttagaccct tgtacaaaga ttcctacata acaaaatgag gtggagaaaa
     4801 tgaaagattt aaggtataga agtgtctttg atattattgg acctgtgatg attggtccaa
     4861 gtagctctca cactgccgga gctgctagaa tcgggaaaat catacgtagt atatttggcg
     4921 aacagcctga ttctgtagat atttatctat atgaatcatt tgcaaaaacc tatcgggggc
     4981 acggaacaga tattgcatta gttggcggcc tgttagggat ggaacccgat gatgaacgtt
     5041 tagctgattc tttgaaattg gcatatgaag ccggtatgga agtcttgttt gttcctaaga
     5101 gtgaaaaagc cgaacatcca aactctgtaa agatgcttct aaaaaaagga acacgaaagc
     5161 tttcagtcac tggcatttcg atcggcggtg gcaatatcca gatttctgaa ttgaatggat
     5221 tcaaaatttc tttaagcatg ggtactccta ccttgatcgt tgtccaccaa gatgttccag
     5281 gtatgattgc taaagtgact agtatacttt ctgagaccaa tacgaatatc ggcactatga
     5341 cagtgacacg ggaatcaaaa ggtgaaaatg ccattatgat aattgaagta gatgatcctc
     5401 aagtagaaga aacggtaaaa agactcaaac agctgccaaa tatcgacagc gtaaattatt
     5461 tcgattaagg agctaaaaaa atgtttttat cgattgaaga attagtagat caagcaaaag
     5521 cttatccaag tgtagcagaa ttgatgattg cagttgaaat ggaaatgagt ggccgttccc
     5581 gtagtcacat catcgagatc atggaaaaaa acctctcagt aatggaacaa tcaatcaacg
     5641 agggaaaaga aggtgttacc tccgtcacag gaattaccgg tggtgatgca aaaaaattag
     5701 atgattattt aaatgaggca gactttttaa gtggagaaac aatcttaaaa gctgtacgca
     5761 atgctgtagc tgttaatgaa gtcaatgcaa aaatggggtt gatttgtgct acaccgactg
     5821 ccggaagcgc gggtgttgtt cctggtgtgc tgatggctgt ccgagatcgg cttgatttga
     5881 cacatgaaca gcaattggaa ttcttattta ccgctggcgc tttcgggtta gtcatcgcaa
     5941 acaacgcttc tatcagtggt gcagaagggg gatgtcaagc agaagttggt tctgcaagtg
     6001 caatggcagc agcagctttg gtttgtgcaa aaaatgggac tcctcatcaa gcagcacaag
     6061 cagtagccat tacattgaaa aatatgatgg gactgatttg cgatcccgtt gcaggcttag
     6121 ttgaagtccc gtgtgtcaaa cgtaatgctt taggctcttc tcaagctttt atctctgctg
     6181 atatggcatt agcgggtatc gaaagtgtca ttccgccgga tgaagtcgtt gctgcaatgt
     6241 accaagtcgg acgtcaaatg ccgtcgattt tcaaagaaac agcagaaggt ggcttagctg
     6301 ttacgccaac tgctaaacga cttgcggaag agattttaaa taagagggcg tgataaagct
     6361 ccttaatttc gagtattaag agattagtta tcttcataac aaaaaacgaa gagaggacta
     6421 attgcccttc tcttcgtttt ttattaatcc atcaatttcg tattttttac ttcatcgatc
     6481 gtttttgttc ctgcaagttg catcgtgatc gacaattctt tgttcaggtg ttcaaaaaca
     6541 gatgttactc cttctgcccc acctagattc aacccataaa tcactggtcg gccaatcgca
     6601 actaagtcag caccacttgc cagagctttg aagacatgct ctcctcgacg gacaccagag
     6661 tcgaaaatca ctggtacacg tttgttgact acttcggcaa tttttggcaa tacttcaaag
     6721 gacgcaggac ctccatctaa ttgacgtcca ccatgattgg atacccagat accatctgcc
     6781 cctgcagaga tagcgattac tgcatcttct ggcgattgta ttccttttac gataactgga
     6841 agatgggtga tttctttgat tgttttgatg tcatctggtg tcaagccttg ttttgcagct
     6901 gcataaattt ctgcgattcc ttttccttcg ccattaccac ttgcactttg ttcgctatac
     6961 gcagctaagt taggcattgg taatggaaat tggaattgat tgatgacgtc ttcttcacgg
     7021 taaccaccga gcgttgaatc tgccgttaaa atgatagctt tagcccctgc ttctactgct
     7081 ttgttcaaaa tgaactcatt gaatctgtcg tctttactca tatataattg gaaaaactgc
     7141 ggtgcgcctg gcgctgcatc ggctgcgtct ttgattgtcg tatttgcata agtgctgatg
     7201 gaaaagattg atccagcagc agctactcct ttagctgtat cagcttcccc tttttcatgt
     7261 gccaatcctt gggccgctga tggcgcttgg ataatcggtg tatctaattc aattccgaaa
     7321 acagatgtat gcaaatcggc tgagtcgatt ccgcgtaata tccgtggcat gattttcttt
     7381 gtattaaaag aagtcgtatt ttctttcatc gtccactcat cttcagaacc accgcgaata
     7441 tagccaaaag ctcctgcttc catgcgctct tttacacgac cttctagtta ggcaatgttt
     7501 acaatatcga ttggatgttc ttcattactt gtatgataag tcatagtaga tccttctttc
     7561 atcttcttga ttacatataa atcatactaa acttacaaaa agtaagcaaa catttactct
     7621 cagatacttt tatcgctatt ctttccaaat aaatcgactt acaagctgat ctacttcttg
     7681 gttttcatga agcatactat gagaggcatt ttcacctgtc actacttttt cctctatttc
     7741 atttccgcgt tgcttcaaca acgcatagac cgctaatgca ctgtttaacg gtactgttcc
     7801 atcactcaag tctgtttcat ctagttgacc agcaatcaag aaaaatcgag tggttatagg
     7861 aatattaccg atcatttgct ggtaatcttg atagcggtta gaaacgacag caggcccctt
     7921 tgctaactca tctgtcagag actgcgcact atcatcgaca aaatcattaa aaggcgcgcc
     7981 aattgctgtt aatgtctgaa cttgtggcaa tgacgtatcc tgtccatacg ttcctaaata
     8041 gcgtagtgca ctaactcctc ccatggaatg ccccactatg ttgactttat ctatgccgta
     8101 gttttctttc agatacaaaa gacaagtttt gatccattct gcttgattcc actcgttatt
     8161 tttattatct tcaaacaata cttggaccat cggattgtct gcttttccag acaaatctcc
     8221 tgttgcttgt atttgtccat ctgttcctac tgttaaaacc aattctttta ctgcttgctg
     8281 atttttttcc aaacgccgca acatcccttt aaaggaattt tctgtcccgc tgtagccgtg
     8341 gaaaaaaagt gttggaatag tggttttttc tatcacttta ctggattctg ccgttttttc
     8401 tttatcagaa acttcagtag tatttatctt cgtcgtctgg caaccactta acgcaaacaa
     8461 actgataagg actaaagctg ctgcagttgc acgtttgcat aaatttttga ttggttctgt
     8521 catacacctc ttcctttcca tatagaaaaa agaaagactt gaaaggccaa tcatatgatc
     8581 gacatttcaa gtctctttat taatcgattt tatctacttg atcgaaatca agttctgtac
     8641 tggtttcacg tccaaacata tcgatgttga ctttcaattt ttctttttct ccgtcaactt
     8701 ctgttacttg tccttctaat ccagcaaatg cgccttcgat gattttcacg acttcgccaa
     8761 cttctacttc tagatcattt tgtcgcaggc tcataccgat agaacgcaag atatgattga
     8821 cttcttctgg aagtaatggt gctggtttgc ttccggctcc gtgtgaacca acgaatcctg
     8881 tcacaccagg tgtgttgcgg acaacatacc atgaagcgtc tgtcatgatc atttcaacaa
     8941 gtacgtatcc tgggaatgtt ttatgaacga tttctttttc tttcccattt ttgacttcag
     9001 tttctttttc ttcaggaaca acgacacgga aaataaagtc ggccattttc atactttgcg
     9061 cacgagattc gatatttgct tttactttgt tttcatatcc tgaatatgta tgcaagacat
     9121 accagttttt ttcaaaagat tccattgaag gctcctcatt tcattgtaaa ataaaaaaac
     9181 ctcagtacac ccgaagtttt tctttcgctt cattatagca ctaaaagtac gttgtggcta
     9241 gcgagttatt ttaaaatcca gccaaataag ctttggatgg ctgtatccat tacgtaaaac
     9301 atcaatccga aaataataga agtttcgatg actactaacg tatctttacg gagttgtttt
     9361 ctgcttggcc aagttacttg tttgatttca tcttttacgc tttttagaaa tttcatgaga
     9421 caacctccct tgctactgtt tatttcgttt ctttatgtaa tgtatattga ttacaatatt
     9481 tacaaaactt attgatttcc aaacgttcgc cgctttttcc ttcactgact gattttgtgt
     9541 agttgcgaga cccacaaacg gaacaagcga gtgctgcttt tttctttgcc atttacttct
     9601 gcccccttgc tgagaatcag acttatactt cataacagta tcacgtaact ttttacttgt
     9661 caatttgtga aataaactat ctattgtttt ggataaaatt atgatatgat taatttgaaa
     9721 ttaagctaaa atgagacaat tggaggtata gcgatgctat taaaaacact tgtatataag
     9781 aaaaaagatc tgacaaccgt tacagagact agtacattag aagaagcatt aaaaatttta
     9841 gaaacttccg gataccgttg tgtgccgatc ttagattctt caggtaatat tttccgtgga
     9901 aacatttaca aaatgcatat ctatcgtcat aaagcaaatg gcggtgatat gacgcttcct
     9961 gtcactcatc ttttgaaaaa cgctacaaag ttcatttacc tggatacttc tttcttcaaa
    10021 gtattcttta cgatcaaaga gcttccttat attgctgtcc ttgacagtga aaatcatttt
    10081 tatggtattt tgacacatag tacgttgctt aatatgctct ctcaatcttg gagcgtagaa
    10141 caaggaagct atgtactgac aattgcttcc acaggaaagc aaggagattt ggctgctatt
    10201 gcaaaaatca tttcaaaaca cagcagcatt gccagctgta ttactcttga tatcgaaaaa
    10261 gacgaataca ttcgacgtac attgatcacg ctaccggcta acactgaagc gaaagtatgt
    10321 gaaacgatcg tcaaccactt agagaaaaag aattataaag tggtagaaat tgaaaatttg
    10381 caaaaccaag aagcataatc aaaaagcgaa aaaagaaaac agctaatctg ctttcttttt
    10441 tcgcttttta taattagaca cgcttttcaa aatcaagcgc gcgatctaaa taatacatca
    10501 atggtgctga aacagccatg acgataaact cacttaatgc aagcgtgcca taggttgtcc
    10561 aaaatgcttg gccacctgtt ggaactaaca tataagcaat cagtcccatg ctgatggtaa
    10621 agaagatcgt gttcaatact aaacgcatct ttacattttt cactttgttt tgcaataaag
    10681 ctgtcaggcc taaagaaagc aatgtttggc cgccaccaaa aaccacatcc atgataccaa
    10741 aaccaaagaa ggcattatag acgatgaccc cgcctaaaac tccccaaagc aatttacggt
    10801 taaagacaac taaatgattc aaactttctg aaatacgaaa ctggatcggc cctgaggata
    10861 caggcgcaac taaaatagtt aacgctagat aaagagccat gatgatgcca tttacaacga
    10921 ttacggataa ccgttgtctg cttttcgttt tttgcatttt ctctcctctt ttctcctagt
    10981 ttttttaacg cgggatggtt gatgaaccgc ggttatgtga aacaaacaat aattataaca
    11041 gacttttcat ttatttcaat ccgtcttatc tgcttttaaa aaagcaaaag cttaaacaat
    11101 gtaaacgttt gctaaaaatc gtgggatatg gtataaagta agtgtaaggg gtcataagaa
    11161 ttcccagtaa agcagacgaa gtaggatctg caagaacaga attgttgtga aacttaaata
    11221 aataaaaaag gatgtgggaa tttgagcaac ctcttactag agaacggtct agcaagcagc
    11281 tgaagatcta agtggacatg ccgaaccttg gaacttctcc ttacgtaagt aatggatcat
    11341 gcgcttgcta ggcttttttg tgtttttcgg tcttcggacc tttttcattt tcctagactt
    11401 ctgctataat aaccaagaat taattttgta acagattttt ttgaaaaaac aagtttcttt
    11461 cattcaaaaa atagaaaaag cttgctaatt attggttaac cagcaaacag gagtattaaa
    11521 atatcttgtt ttattgaata gcaggaggaa atcatgaaat tccattatca tatattgatc
    11581 aatccatcgg caggcagcgg aaatggctat aaagtagcag aaagaatttt accggtatta
    11641 aaaaataagc atattgacta tacacttcat tatagtgaat acaaaggaca cgatgctgag
    11701 attgctgaaa ctttggcaaa agaaacattg attccttgga tagaagaaga acagaaaaca
    11761 atcgatactt tccccctttt gatcatcgta ggaggtgacg gcaccttaca tcaggtactt
    11821 gatactttct atcagatgga agtagaattc ccagttgctt atattccagc aggttctgga
    11881 aatgattttg ctcgaggggc aggccttcca aaaaatccca aaaaaggact tcagctgatt
    11941 ttagcagctc aatcaccaga aaaagtccac ataatggctt atgaagaaaa aataagtgaa
    12001 aaaaaaggaa tagctgttaa taatttcggt ataggattag atgcagctat cgtccatgcg
    12061 acaaatcatt cttccacaaa aaagcgattg aacaaataca acttaggatc attttcgtat
    12121 ttattctcta ttctccgtgc tttattcact caaaaaggat ttccaattct ggtagatgca
    12181 ggaggaaaac aattaagttt ttcaaacgca tttttatgta cagcaacgaa gcatcctttt
    12241 tttggcggag gaatcgcgat tgttccaact gctgatgcgt ctaaaccagt cattgatttt
    12301 gtcgtggtcg aacgaatcaa tttctttaaa atcttgtggc tcattctttt acttatacag
    12361 aaaaaacaat tgaaatcgaa atatttccat cactacacaa caaacagatt acgtatcgtt
    12421 tcaacaacac cgcaacatgg tcaagaagat ggagaagaaa tggaaaaacg accttttgat
    12481 atcacaataa ccacaactaa acagttgttt tggtgttaat aaaagaggtt ttgtcagaag
    12541 ctacatctgg aacaccgttt attcggaaaa aaggactgtg acacgacttt tgtcacagtc
    12601 ctttttttac tatgcagtta attctttgcc caattgggtt gccatcgata gtttctttct
    12661 ttacccaaaa taaattggat cattgaaaac accgcagcaa aagcaactaa gacacaacca
    12721 ttgaaccaaa aagattctgc aaatgtttga ttgtaactga gtgtttcact gatcaagtta
    12781 ttattttgaa caaaccaata tgttggtgtg aatgccgcta ttttgctaac catttctggc
    12841 aagtattctg ctggtacaaa caccccaccg ataaagcaac tgcctgtgat aaaaatatct
    12901 ttgatcccac cgctggtttc tgagtttttt accaatgaag tgatacaagc cgaaaagcta
    12961 atgatcggca ataaaaacaa gaaagtattt aaaatatgat accataccgt tatatcccac
    13021 gaggtgtgga tcactaacag aaccattaga agtaagaagc cactgaacaa cagcagcgag
    13081 taactaatca aactaaaggt tactttgcga ttcaagtttc gtttggtaat gggcgcactc
    13141 tggtttcgtt tactgatctc tttgcgatta aacgctagat tgatgacgct gaaaccgcta
    13201 aaaatggata gaaacaaccc ataagacaag aaattaaagg ttgtgcccgt caacagcttt
    13261 ttcatccgct gactatatcc cgcacttagc tcaacttctc cagtaaccga gagtgccatt
    13321 tgtgtctgtt ccatagctgc atcgattgtt ttatcttctt cttgaaacag aatcagtgtt
    13381 tgcaaaaagg tgttcacttg ctgtgtaaca actgttttag aaaaggcatc tgggcgactc
    13441 tgtatcttga tcgtcgccat ctttcctact tcgacttgtt cggtaaagtc tgcaggcaga
    13501 tgtaagataa tgtttacacg attaaaataa agagcatcat cgatttcttt ttgtgaggtt
    13561 ccatccaatg taacgactgt ttccttgtca ttcaaatatt tacgaaggcc atcgactaca
    13621 ggcgactctt cttcactaat caaggcgatt ttcgcatctt gcagagaggt attttcaaca
    13681 tcaatatttt gcgcaaaaaa gaatgtggca acaacagtga taattacacc tatcagtaaa
    13741 ttcgatttat ttgctttaag caatgtccaa atcgttttat aaagcgtcat aacgcacccc
    13801 tctttccatc caagcatttc ctaacgcaaa aaggaaaata aatccgatca accatagaag
    13861 attggtataa aaactagcca aatcagtgta gaaataaagt ttatacaaac tttcgctgat
    13921 caagttgacg agatttaatt gaccaacaat cggaatatga agatcaatcc aatacttcac
    13981 ttgttccgac cccatcatac cagcaaaaaa gctcatcgca atcgtcactg aaatcaagat
    14041 gctatccttt tgcaccagat tcatttttgg tatcaaatta ccaaccaatg tacctaacaa
    14101 aatcgcacac aacgacccca gagcggtaac aatcatcaac cattgccaac gttcaccaaa
    14161 gtctacttga tagataaaac gaaagacagc taacataata taaatctctg tcaaaacaat
    14221 cgtaaagctt gctgccaaat tagcgattga aactaacgat tgctttctag gaatcaagga
    14281 caagcgaata ccatttgacg attgatttgc ttgctgatcc tgtacattgc ggacccccca
    14341 catcgtcccg tagagaatcg ccattccaat cagagtgaag aaataaaaac ttttgaaact
    14401 catattacga cctgcatgaa cttcttgaaa agatacttct tgttcaaaaa cagtcatgat
    14461 gggcgcgaga tcaacgttct ccacttctgc cactttactc gaaattatac caatacgctg
    14521 taaaaattga tccatgacgt ttttcatcaa cgtctgactc attcctgctt gacccacatg
    14581 gagcgaaaca gatgaaccat caatgagata aaaaccagcc aaatcatctg catttagctt
    14641 ttcattcgct tctacttccg atagttcctg tacttcaaat aatggtttat cattgacttc
    14701 gacttgttct aagactgtgg taaaattttc cgaaagcgct gtttgatccg ctttgattac
    14761 aattccagta ttaatcgttt caagattgcc accttgatca atctcgccaa aggccgccat
    14821 aaacatcaat cctaaaagaa ttggaaaggc caatgtccaa aacaacatgg atttatttcg
    14881 cagcagtatt tttgtgcgat atttataaaa atgccaaaac attatttccc ctcctctagt
    14941 ctctcaaatc cttgccagta atctctaaaa agacatcatt caaggacgct tctttggttg
    15001 ctatttttcc gtaactgatc ttttcttctt gtaagattgt aagaatcggc aacaatactg
    15061 tatttagttc agtagttgtt acttggagct gacctttttt ataagcgatt ccatcagaag
    15121 ctattgttcg ctgaatcgct gacaacacat tttctgggag taatgggatg tcgatcgtca
    15181 aacgatccgc cgtccgtacc atatctttta gagattcttt ggttccttta gcaatcacct
    15241 gtccttgatc catgatcacg atctggtcgc aaagaatttc tacttcttcc atgtaatgtg
    15301 ttgtatagac aatggttgct ccttgtcgat tcagctcctt gatactttct aaaattttat
    15361 tccgactttg ggggtctaca gctactgtcg gctcatctaa aaaaattaac cgcggcttgt
    15421 gggcaatccc acatgcgatg ttcaaccgac gtttcaagcc accactaagt tctcctggac
    15481 gaaacgaaac atatttttct aatcccacta attgaatcac ctcttcaatc aaggctttcc
    15541 ttttctcttt ttcggaaaca tataaaccgc aaaaataatc gatattttct tgcacactta
    15601 gttcgtcaaa gaccgcaacg tcttgaggaa cgaccccaat cattgccttg acagcataat
    15661 tttcaggagt cattgttgtc ccgaacaatt taatggttcc tttgtcataa tttaacaaag
    15721 aaagcataca gttgatggca gtcgatttcc cactgccgtt tggacctaac agacctaaga
    15781 cttctccttc ttgtacttgc agatcaaaat gattcaatgc tacaaaatct ccatagcgtt
    15841 tgactaattt atcaatttcg ataatcatca tgaaaaactc cttccaaatt tcttgttgat
    15901 ctgactataa taaagtgcaa gaaaagaaac tagtgacgat tggctaaacc acacatgaca
    15961 tttgtcacaa tgaaggagtc tgctatgcat aaatctcggc tactcaacga aactagcttt
    16021 ctactggtta tctcattgct tttgctggta agtcaaggat tcgttgctat gcaagttatc
    16081 tatttattga tcgccgtttt attcactggt gtcagttatt tactgcctac aaagccaaat
    16141 tggattttcc aagttattct atttctttta tgtgcctttt tacctgatct tgtcctgttt
    16201 ctgccagcga cgatgcgagt gatcgcccct gaagaaagcc accttgatta cttgtttttg
    16261 ttaggttttt tgttgatttc gacggttggt aatcatcccc ccatcaaggt cgcctttgtc
    16321 gtaattttgc tgtttttttc cttttactta cgaaaaaaag atcaacgaac gaccgatctt
    16381 gagaaaaagt acattgtctc caaagatact cattgggaaa aacaacaaga gttatcggaa
    16441 caaaataaag cattggccct gtcacaagaa acacaattag ccttgcaaat tgtggaagaa
    16501 cggaaccgaa ttgcccgaga catccatgac aatgtcggtc atctgctgtc aagtgcgatt
    16561 ttacaaattg gtgcgcttga aatgatcaat caagaaccaa aactaaaaga accattagaa
    16621 aatttaagtg ccaccgttca cactgggatg gatcggattc gtcaaagtgt ccatgacctc
    16681 catgagacat cgatctcttt tcaacactcc ttagagtttc tgatcgctga ttttccttct
    16741 tctgttacga ttgaaggaaa cttgtttgaa ggattgaatg aaacgcagca aaactgcttc
    16801 ttaatggtca tcaaagaagc cttaaccaac agtatcaaac atagtcatgc caaaaccgtt
    16861 gttttacatt ttcgaacgtt gccggccttt tatcgcttgc aaatcacgaa tgatggcatc
    16921 agcccacaaa aaaattctgc tcccggtatt ggtttgaaaa cgatgcgtca acgtgttcaa
    16981 gaacaaaagg gacaagtcca tattttccaa gaaaaccagc aatttcagtt aacgatcatt
    17041 ttaccaaggg agggacacca atgataacag tagtcattat tgatgatgat ccatttgtga
    17101 cgaaatcgtt acaaaccatt ttagaaagca cgaaagagat ccgtgtgtta ggcattggtc
    17161 attgtgccaa ggatgcgcta gtcttatacg aaacccatcg tcccgatgtt ttgctgacgg
    17221 acatccgcat gcctaaacaa acgggcatcg atgctgccaa agaaattttg gctaaatttc
    17281 ccaaagccat ccttttgctt ttgacgacat tcaaagatga agaatacatc cgcgaagcat
    17341 tttccatcgg tgtcaaaggg tatttaatca aacagaactt gcaagcgatt attccttctg
    17401 tcaaatccgc ttataatgga caggccgtat ttggtaatga aatcatcgag actttcactc
    17461 aaatgatgaa gaataaaccg acgattgata gtcatgctta tgcttctttt agtgaacgtg
    17521 agcttgcaat catcaaagaa gtagctgccg ggaaaaacaa taaagaaatt gcagatgcct
    17581 tgtacttgag tgacggcact gttcgcaatt acatcagcca attactagaa aagctagatt
    17641 tacgggatcg cacgcaattg gctatctatt tttatcagca tttgcaataa gtcttatcta
    17701 acacaaaaaa acctgaatga tgctccttcg catcattcag gtttttttca catcttctat
    17761 tcaattgtcc cgcaaaccat ttcctcacga ttgaaagaat caaggattcg cagggctgcc
    17821 aggattcgtt ggattgttcg gcgtgttagg cggaaaaata ggcgccttgt ctaacgtttt
    17881 ttctagtttt gattcttcac gatcgtctcg ttctacatca ggactttcat tcaatttgaa
    17941 ttctttttcc atctcatcta actttttaaa gtcattgcca gattcttttg ctaatttcgc
    18001 taatttatct tcggtttctt tagccataaa gtaacgcccc ccttttgatt tcagcttagc
    18061 atatcacact gaaaaaacaa aagaaaacgc taaccaacga tcatttttag ctatttctca
    18121 tctttaaaat caagcatttt ttgccggtat aaaatcgtag ggagaactgt tttccatacc
    18181 aatcatcgga tctactaact ggttatcgat cacttctgct gtaagaacga aatctttaga
    18241 aacagcaggt tctacctctt ctttcttttc ttctccaaca aatgaagaaa acagatctgg
    18301 ttccactgtt tccattactt ggtttttcgt ttcattcgtc ccactgtcat cttctacagg
    18361 tagcttttct tttacaaccc gtggctcttt tgtatagttc ccatcttcaa atggatcatt
    18421 tccgaccaaa gaatcttcat aagctgctag tttggtccgg agagtgatat tcattttatc
    18481 attatccatg atccttgatt tcaacgtagt catccggctt gctgattcat tcgctacaca
    18541 agtgacatag gcttgctctt cccctaacaa aatcaacagt tccttactgc gagtcactgc
    18601 cgtgtagagc aaattacgct gaagcatccg ctgatattga tggaccatgg gcaaaataac
    18661 catcttgaat tcactgcctt gtgatttatg gattgagcaa caataagaaa gagtgatttt
    18721 attccattcg tttcttttat aagtgacctc atttgcatca aactggataa ctaattcatc
    18781 cactttatct tcagaatctt ttgccaagat gatacccgtg atttctccca tatctccatt
    18841 gaatacattc aattcgggag tattgactaa ttggaggact ttgtctccta tccgataaac
    18901 ggtgtcgttc caatgtactt ccttttttcc aggtttccct ggattgaata tctcctgcat
    18961 cattttattt aacgcatcga tccctgcagg tccccgatac attggtgcta atacttggat
    19021 atcttgtgca cggaatccct tatttttcgc tttttccaca attttagaga tcaagggttc
    19081 aatttgataa gcatcacttc ggaaatagga acgatctttt tgattctttg tgaaatcttt
    19141 tggcagacgt ccttctttga tctcatgggc aagaggaata atactagaac catcaccttg
    19201 ccgataaatc tgattgagtt ccattttggg aatttgttcg atttccaaaa gatcatggag
    19261 tacttgacca ggaccgacag agggaagctg atctttgtct cctactaaga taacttgcat
    19321 attcgtcgga atcgctttga ataatgtgtt agccagccat gtatcgacca tagacatctc
    19381 atcaacaatc aaaagtccgc cttctaattc tttcgtactg actgaaggtg ttttttcccg
    19441 tccattcagc cccagaagac gatggatcgt actggcaggc agacctgtcg tttcgttcat
    19501 tcgtttagct gctcggccag ttggtgctgc aagtagaata ggaaaaattt cttgggtata
    19561 ttttgaagga tccaattcca gtccgttcaa ctctgcaaaa agattgacga ttccgttgat
    19621 cacagtagtt ttccccgtac ctggtcctcc tgttaaaata aatagagggg aacggattgc
    19681 ttcagcgata gcttgttctt gcgaaggtcc atactgtatg ccgaatattt tttcgatacg
    19741 ccgaatattc ttctgcactt ctttttcagg ataatggatt tcttttttac gactgagaag
    19801 gcgctggatc gaattaccga tcccccattc agaaaagtac aaactgtttt cgtaaagatt
    19861 agtctcttct tgttgtatct ttccttcttc aacaagttgg ataattacag tagcaacctg
    19921 atcaggctgg atttcaattg ggcgacttgt ttctaaaata cggatcgttt gctgaagcaa
    19981 tgtctccgcc ggtacgtatg tatctcctgt ttgaactgca tgctgaaaaa tctgatgcaa
    20041 gatagctgct cgaattcttc tcggtgaatc tgctgcaata ccgatttgtt cggctagatt
    20101 atctgcacgt ttgaacccga ttccttctat gtcttcgact agctggtacg gattttcttc
    20161 gatgatatcc aaagactcgt tcttataggc ctggtagata ctgaaagcta actgactacc
    20221 gaacccgtaa cgattcagtc ccatgatcac ttgatccatc ccgtggttta gccgaatcgt
    20281 ctcagcaagc atttctcttt ttggcttcgt caaaccggag atttgtttta ataccgaagg
    20341 atcagcaatg attttttcga tagcatcttc tcccagcaat tctacaattt tttctgctgt
    20401 tttcttcccg attcctggga atttttcgct ggaaagatac gctacgatcc catttgcagt
    20461 tgttggcttt tcttgctcat atctttcgag tttaagctgc cttccgtatt taggatgatc
    20521 cactaattct ccataaaaac gataagtctc attttcttga atatcgccga aacttccggt
    20581 gactacaatc tctttttcac ggtaatcagc tgtggtttca ctgatgtgta ccaaaagcac
    20641 tttgtaaaaa ttactaggat tttgaaaaaa aatcgcggca actgtaccaa cgaaataagg
    20701 catttcttct ggctgcatcg gaagccgcct ccttcttcta tagtaaaggt tctggtgctg
    20761 tttcagttcc taaaaaatct gtttggttcc atactttcag ttcatatggc aataatctgc
    20821 aaggctcttc agtctctaaa atcgtcaaac tactattgaa caatccaccc atttcacgca
    20881 actcacttag ctcttttcct gtcatccatt gaatggctgc agttaaagaa gctccatgtc
    20941 ctacgaataa aagtgggcca tctttatgtc gggcaaccgc atctgctaca acagtttcga
    21001 tacgtgtgat tgccgattca atcgtttctc cgtgaaaagg agaaggatca tacagatcta
    21061 aatgattacg caaattcggc agattatccg gatatttctg gtacatttca tcgatcgact
    21121 gaccttctaa agtacccagc cctagttctc ttagcttatc tgtataaaaa atctctaccg
    21181 gacgagcaag ctctttgttg ataccttctg ctgtatcacg tgctcgcttt gctgtactgg
    21241 aataaattgc tttaaaagga acatatttta cctttttccc aaatgctttg atttcttcgt
    21301 aactttgtgg caaaagcgga gaatcgccat tgctcccttg aaaacgtctt tcctgattcc
    21361 attgtgtctt cccatgtcgg gtaaaatata gttccatacg cagcaaactc ctgtctttct
    21421 tttattttac tctataataa actgtctaat ccagcttgtc attttcatag tgatgcgtca
    21481 attctaaatt agggaatcct tgctggcgaa gtgcttcata cactatcatt gcagctgtat
    21541 tcgataaatt caatgagcgg acatgctcat cattcatagg gatgcgtaaa cacttttctt
    21601 cgttttcacg cataaatgtt tctggcaaac cggttgtttc ttttccaaat aaaaagaagt
    21661 gctcttctcc atcagcaaaa ttttcgtcac tatatacttt atgggcaaat tttgagatca
    21721 aatgtagttt acgattacct aaaaatgtta aaaaagcttc taaattactg tggtaagtaa
    21781 tatttacatc attccaataa tccaaccctg cccgttttag atgtttgtca tcagttgaaa
    21841 aacctagtgg ctcgattaaa tgaagcggag tattcgtggc tgcacacgta cgggcaatat
    21901 ttccagtgtt tgcagggatt aatggttcaa ataaaacgat atgattcatc aggatcattc
    21961 ctctcatatt tatgttcgtt tctattttct gacaatttga tttttgcata aaaaaaaacg
    22021 tatcaactcg gtgtcagtca ctgcgagtcg ttacgttagt gaagctcttc tcactaactt
    22081 ttatcagaac aggaacctga taaaaaccaa tgaaaaaaca acttgttgct aatataacac
    22141 cctcaacttt tttttgcaac ctctactcat taaaaactgc tagaaaaacg ttttctctta
    22201 gttcctttta ataattctat gtcaattgta atcttcttca aaggattctt atataagtct
    22261 gctttgattc gctcttctac ctgccattct aaaggtgaca tggccaaaag atctttttga
    22321 ttttcctctg gtatctcaaa ttcataggtt acacgctgac gttcagttaa tttcattgct
    22381 tcagcaaact tggctactac acgttcattg gaataactct gtttttcagg ttgattcgga
    22441 taaaaagcct gacgtagttc ctttaaataa ttttcttgtg gcaccacctt caacacttct
    22501 ccatcttctg agagtacacg ttggaattct tggtaatgag aaggcgaaaa gatatttaaa
    22561 accgcgttga atgtttgatc agcaaacggc aaattggtca aatcagctac acaccaaaat
    22621 gcttcggcag attgttctgt agctgcgtag actccttctt ttgaaatatc aaatccaaca
    22681 gcattttgaa tctttttcaa ttttgaaagt tcattcaaga agctaccttc tccgcagcca
    22741 acatctaata gcgagacttc ttcaggtaaa tgctgcgcaa tagtctcaat aacgggctga
    22801 tacatgccag attggatcat tctacgcctt ggaacgaaca ttccttgatc gtattctgtt
    22861 ttaaccgcat gggataaaaa ataaagtgtt ccttttttgg aaaggtcaaa acggtgatta
    22921 tttttgcata tcaagccatt atcagacttt tttacttctt cttggcaaac cggacagcgg
    22981 aacagctgat gattttctgc caaaaactgg cgtcctcggt caattttttt taacatagtt
    23041 ccccctcatt tctgttttat cttaccgtta gatgctcact ttggcaactg ctaaaaatat
    23101 gatacaatgc ctagaagaaa gtgaaagata aaggagaaga ttagatgatc aaagaatttt
    23161 gcgctgaaaa ctacacatct attcctctag caattgctag aggagccgat cggatcgaat
    23221 tgtgtgacaa cttagcggtc ggtggcacaa ccccgagtac tggtgtgatc gaagaaacgt
    23281 taagttatgc tggggaaaaa gatatccccg taatgacgat tattcgtcct agagggggcg
    23341 acttcgtcta taacgatatc gaattgaaaa tcatgcatac tgatttgatt gaagcaaaga
    23401 aattagggac ggatggcgta gtatttggtt gtcttactcc ttcaggctgg ctagacgaag
    23461 aggccttaga actattgatc gaaacagcag aagggttgca gatcaccttc catatggctt
    23521 ttgacagtat tcctgaagat cggcaattcg aagcaatcga ttggctggta gaacatggtg
    23581 ttgaccgtat cttgactcat ggcggtcctg ctgacaaaac tattgtagaa aatttcccac
    23641 ggcttaaaga attgattgcc tatacagatg gccgcttaat cattttgcct ggtggtggtg
    23701 tcactgcaga gaatgcagca catgtagcta aagaattgaa cgtaagcgaa gtacacggaa
    23761 ccaaaatcgt ggatcttcag ttataatctt ataaacaaaa aaacatttgc aacaggcata
    23821 atgcttattg caaatgtttt tgttttttta cataaaatag cccataaata aagaagcaat
    23881 attgaaataa atcagtacac tcgtcaaatc gctcaaagtc gtgataaagg gaccgcttgc
    23941 tactgctgga tcaaatccta gacgatccat tagcatcggg attaaactac ctgcaagatt
    24001 cgccactgtg attgcaaata gcatagccat ccctatgaca aaccccaaga taaaattatg
    24061 tttccaaaaa cctacgatta gaaaaatcgt gatccccgta acagctcctg tgacaacgcc
    24121 ggtcaagact tcagccaaaa caagtcgaac aaatccatga tctttctcat cagaaatcgc
    24181 aagtcgacga acagccacag ctagcgactg ggttccagca ttaccggcag ttcctgtaat
    24241 cagcgaaatg aagactgcta aaatactcgc ttcacttacc aagtcttcat agtgactgat
    24301 taaagaagcg gtcgacatac ctaaaaacaa cagagtgatc agccaaggca aacgtcgaga
    24361 cgctgccttt actggatttt cattaatttc ttccacattg accccggcaa gtcctgaata
    24421 atcgctagca gcttcatcat caataacgtc gatgatatca tcgactgtga cgattcctaa
    24481 caaatgatca tcataatcgg taacaggaag agctaagaag tcataatctc taaatgtttg
    24541 tgccacatct tcttggtcgt cgcctacatg aactgaaagc actcgttcgc tcatcagatc
    24601 agaaatcatt gcgtcgtcat cattcacgat taggtcgcgc agagaaataa cccctactag
    24661 ctgaccttct tgattaacga catagatgta atagattgtt tcagctacat ctgcttcgtt
    24721 cttcagcacg tacatcgcag agcgaacagt ttgattggct acgatcgaaa caaattcagt
    24781 tgtcatgatc gcgccagccg tttcatcctc gtaatggaga agttccttga tttcactggc
    24841 atcgtctgcc gacatcaaac ttaaatattt agcaatttgc tttttatcca gcgtattaag
    24901 caagtcaaca gcattatctg tatacatctc agaaagcata tcagctgcat agctaggacg
    24961 catttcatct aggtattctt tcatatgttc gttgtcttct tcaatcacat cgaacatatc
    25021 cgctaattct ttaggcgaca gataggaata gatcagctgc ctgttctctt ctgtaagaga
    25081 ttggtagaat tgcccctgct catagatatg catctctaaa aaactgtttc tgaatgcctg
    25141 aatattattt tcccttaaat cttgagtcaa ctgggcaaat ctttcctcta attcttgatt
    25201 ttcatccaaa ccaatctcac ctcgccttat tcttcaaacc gtctcacaac tgcctccatg
    25261 tctgcggcta aaggctgctt gaactccagc aactcttctg taaatggatg gtaaaaacgc
    25321 agatcataac aatgaagggc ttgtcttttt attcccagat ccattcgccc gccatacata
    25381 tcatcaccga gcagggcaca gccaattgcg gaaaaatgaa ctcttatttg atgtgtcctt
    25441 cctgtatgca gctggatatc aactaacgcg atttctgtta gtcgcttctc tatccagtac
    25501 tcagtctgtg ccgaacgtcc ttcatcagag accattcgct tcaatagcga agaataatct
    25561 cgggcgatcg gcaaatcgat atgtccatgt gccgataaat ctgaaagcct tccactgact
    25621 aaggcctgat attttttcac aaacttcttt tcacgaagtt gcatatctaa ttttgcgtgc
    25681 gcaaatccat gctttgcaaa aagcatcaag ccagatgtat cccgatctag tcgcgtcacc
    25741 acatggatta cttggttttc gtaaccttgt tttttataat aagcctttac acgattagcc
    25801 attgtcccat ttggatggta ttgggcagga atagacgaaa cgcctgctgg tttatttact
    25861 accagaacgt gttcatcttc atacacgata tcgatcggtg tttcatccaa aagtacggtt
    25921 tcatgttctc cttcagcagg aatcacaatg gttaccttat catccttagc tagagaaaac
    25981 aagacatttt ctacttgatc attaactttg atttgaccgc cttgaaattt tattttcgct
    26041 aacagaccct ttgagatccc tttttctttt aaaaaatatt ttacttgctg agaatgatct
    26101 ttctcataat cccatgtaaa ctgcatatca atcctcacta ataaaggcat ctttcacacg
    26161 atgccaaaaa tgcatatgtc gataagaagc aaaatggatc cgttcttctg ctattcgata
    26221 ataaattgag cggatatttg cttttgcgac attcaattga tcaaccgtta caagataatc
    26281 ttctgtattt tccaatttta tttctagcca ttcatcatgg gcaatcacga tcggagaacc
    26341 caaagtacga aacactcgat tattcagtga agcaatctct gccaactgaa aggcattgat
    26401 actaggatgg atcacagcac cgccgacact tttattatat gctgtcgaac cagtaggcgt
    26461 agaaatcgtc aatccatctc ctctaaaact ttcaaacagc tcgtttttga tgtatacatc
    26521 ggctaccatc gtacgattgg cccgtttaat cgttgattca ttcagtgcta aaaaatgctg
    26581 gtccggtgtt tcatccaaat aactgatccg aacatccagc aagggataac taacactttg
    26641 ttcccggttc gttcgtaagc tgtctactag ttcttccagc tcgtaatccc gccaatccgt
    26701 gtaaaaccct aaatgacctg tatgcacgcc caagaaacgc acatcattca gacaatgact
    26761 gtagtgatgg aacgccgaca ataatgtgcc gtctccgccg actgagatga ccaattctgg
    26821 ctcatgatca tctcgttgaa tccctgcttg ttctaaaagt gcgttcaaac gttctgtcac
    26881 ttctatagtc tttgcttctt tattatgaac gatcgctact ttcatcccct gcctcctctc
    26941 tttcgctttc tttcttcaaa agcgttcgat agtagacatc atctgatttt cctcttccat
    27001 gggagaataa ccgctgtgct tcttggattt cttcacggat actggacatc tcttcatcca
    27061 actgataggc agcttctgcc gcacgctgaa gtcgttcact catttcttct ggaaaaacgc
    27121 cttgatattt ataattcagc gaatgctcaa tcgttgccca gaaattcatc gcaagagtcc
    27181 gaatctggat ttccgccaaa attttcttct caccgctgat cagctgtaca ggatactcga
    27241 tcaccagatg ataagaacgg tagccacttg cttttttatt tgtgatgtaa tctcgttctt
    27301 ggagaacttt catgtctttt cttttacgga tgatctcaac aacttgatgg atatcttcta
    27361 caaactgaca catgatcctt aacccggcaa tatcagacat ttcttcttct aaacgtccca
    27421 ttggaatatg ccgtaattgt gattttgtta caatactatc gactggcttg actctcccag
    27481 tgacaaattc aatcgggaca tgtcgatttt gttcacggaa ctgtttgcgg atcccccgca
    27541 gtttcacttt caattctgat acagtttgtt catatggtgc taaaaatgct tcccaatctc
    27601 gttccatagc tgatacctct acttcattaa cattcattgt ttattctacc atatatgcaa
    27661 agttttttcg tactcttgat ttatgcattg accaaaaaaa gaaaattagg caaaatagac
    27721 aaaaaggagt cgatcaaatg acacaaatgc ttgaaatcga atataaatct atgctgacaa
    27781 agcaagagta cgagatattc atcgctcact atcaattaac agataaagat ttccgtgtac
    27841 agactaatat ttatttcgat accgttgatg aacagctgaa gaaacaaaat tgtggcctac
    27901 ggatccgttt cgttggtcat caagctgaat atacattaaa aacacctgct gaaaaagggc
    27961 gtctcgaaac aacggatacc tttataacag aagaagcaga agcatttatc ttcgaaaaac
    28021 gtctgcctcg ttcaggcgca gtttttcaaa aactttctga tttgaatatc gatcctgctt
    28081 cattaatcca aaccggaaag cttacaacaa aacgagctga attccctatt gaagaaggac
    28141 ttttagctat tgatgaaagc tggggagaca atctacacga ttttgaactg gaactagagg
    28201 taggggatgc atctgtggga aaaattgctt ttataaattt tttaaaacgt ttttctttac
    28261 cttatcgtcc tgctaagaac aagatccaac gtatgcttga agctaaaaat tagaaacctt
    28321 tcattttcac aaggcacttg agttcatttt tttacttttt tctgttaagt tataggatgt
    28381 gaaagaatct agtcactagt tgtatagaat ctattttggg ggagattcat atgatcgaaa
    28441 tctatttatt tgttaatcct ttaggggaat actgtttcga aatggaacaa cagttattac
    28501 aatttatcga agaagagtac gggacaccat caaaagaaaa gatgcaattt cgctttcttc
    28561 ctttggtcaa tttgcagaca attggggatg tcatgcaaca aaaaggaatt tctcagaaca
    28621 atcttgagga acgtaatcac ttattctcaa tgacttattc tgcagcattg gactgcaaag
    28681 cggcgcagtt tcaaggaaag aaaaaaggcc gccgtttttt gatcaaattg caagaagcag
    28741 tcggctgtaa tgacatacca tattctcaag aacttgctga atccatattt gaagaaatcg
    28801 gcggcgattt gaatatgttt aaagaagatc gtcagtctga cttcgttaaa gaagtattct
    28861 tttctgatca aaaaattgct cgggaaatgg gcatcacaaa acacccttct gcagtcgttt
    28921 acaattatgc ctgcgaccgt gattttggtg ttttagtaga agtaaaaggt acaagtagcc
    28981 tttggagaat cacagagctg tgtaaaacaa cagataactc atatcaagtt tttaatggtg
    29041 aacatttaca tacaaatgat tctcagttcc attcaagaac tagccattta cacttgcttt
    29101 ctaattaaaa
//
LOCUS       DACEUZ010000021        28835 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.021, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000021 DACEUZ010000000
VERSION     DACEUZ010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28835
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.021"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(54..1283)
                     /gene="rpsA"
                     /locus_tag="IXG44_000815"
     CDS             complement(54..1283)
                     /gene="rpsA"
                     /locus_tag="IXG44_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HAQ1657551.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(1418..2098)
                     /locus_tag="IXG44_000816"
     CDS             complement(1418..2098)
                     /locus_tag="IXG44_000816"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HAQ1657552.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTREVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(2147..2782)
                     /locus_tag="IXG44_000817"
     CDS             complement(2147..2782)
                     /locus_tag="IXG44_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ1657553.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(2848..4251)
                     /locus_tag="IXG44_000818"
     CDS             complement(2848..4251)
                     /locus_tag="IXG44_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HAQ1657554.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(4238..5269)
                     /locus_tag="IXG44_000819"
     CDS             complement(4238..5269)
                     /locus_tag="IXG44_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1657555.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            5300..5521
                     /locus_tag="IXG44_000820"
     CDS             5300..5521
                     /locus_tag="IXG44_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HAQ1657556.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(5686..6423)
                     /locus_tag="IXG44_000821"
     CDS             complement(5686..6423)
                     /locus_tag="IXG44_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HAQ1657557.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(6576..7916)
                     /locus_tag="IXG44_000822"
     CDS             complement(6576..7916)
                     /locus_tag="IXG44_000822"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HAQ1657558.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(8153..9676)
                     /locus_tag="IXG44_000823"
     CDS             complement(8153..9676)
                     /locus_tag="IXG44_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HAQ1657559.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(10054..10665)
                     /locus_tag="IXG44_000824"
     CDS             complement(10054..10665)
                     /locus_tag="IXG44_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ1657560.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(10788..10913)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(10995..11711)
                     /locus_tag="IXG44_000825"
     CDS             complement(10995..11711)
                     /locus_tag="IXG44_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAQ1657561.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(11717..12286)
                     /gene="scpB"
                     /locus_tag="IXG44_000826"
     CDS             complement(11717..12286)
                     /gene="scpB"
                     /locus_tag="IXG44_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HAQ1657562.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(12270..13067)
                     /locus_tag="IXG44_000827"
     CDS             complement(12270..13067)
                     /locus_tag="IXG44_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HAQ1657563.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(13037..13408)
                     /locus_tag="IXG44_000828"
     CDS             complement(13037..13408)
                     /locus_tag="IXG44_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAQ1657564.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(13427..14314)
                     /gene="xerD"
                     /locus_tag="IXG44_000829"
     CDS             complement(13427..14314)
                     /gene="xerD"
                     /locus_tag="IXG44_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HAQ1657565.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            complement(14410..14883)
                     /locus_tag="IXG44_000830"
     CDS             complement(14410..14883)
                     /locus_tag="IXG44_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAQ1657566.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
     gene            complement(14988..15851)
                     /locus_tag="IXG44_000831"
     CDS             complement(14988..15851)
                     /locus_tag="IXG44_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAQ1657567.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(16047..18146)
                     /locus_tag="IXG44_000832"
     CDS             complement(16047..18146)
                     /locus_tag="IXG44_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAQ1657568.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(18366..19475)
                     /gene="rpoD"
                     /locus_tag="IXG44_000833"
     CDS             complement(18366..19475)
                     /gene="rpoD"
                     /locus_tag="IXG44_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HAQ1657569.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(19499..21340)
                     /locus_tag="IXG44_000834"
     CDS             complement(19499..21340)
                     /locus_tag="IXG44_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAQ1657570.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(21522..23915)
                     /locus_tag="IXG44_000835"
     CDS             complement(21522..23915)
                     /locus_tag="IXG44_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ1657571.1"
                     /translation="MFLILEHARLKHVIIFESWRKMEKKKMNGLTQQEVVQKMASGQQ
                     NDYQEDASKSTKQIFSDNILTLFNFLNFGIGICLLFVGAYSNMAYLAIIVVNIVIGIY
                     QEIHARNLVRKLSIVSQSEVLVIRDGKEQSISTKELVLGDLVKIGAGEQIPADLQVIS
                     GRAEANEALLTGESDLIVKETGAELLSGSFLTSGSVMAEVIRVGADNYAVRLTSEAKT
                     HKAIRSELVDSIRKVSKFTSFVIIPLGIILFLEALFIRDASIKISVIASAAALLGMLP
                     KGLVLLISIALTTGVTKLAKKRILVQDMYSIETLAHVDTLCLDKTGTITEGKMSIQRV
                     EALHPLYEKAIPEIMGTYLAESSDNNITMRAMRDYFELSNRYDVTNKVAFSSDRKWGA
                     MEFPELGVVYVGAPERLVEEDRLPDSLKTAQANGYRVLMLGIAPNQQLNEEKPAFVEP
                     VALFEIDDPIRKNAQETLAYLHEEGVDLKVISGDNPFTVSNIARRAGLPNYDAYVDLS
                     QITEEMEVREAAHRYTVFGRVSPQQKKLLVNELKESGRTVAMTGDGVNDVLALREADC
                     SIAMAEGDGATRQIANLVLLDSDFTTLPEVLFEGRRVVNNVTKVSGIFFIKTIYSFIL
                     SIICAVTAIGFPFIPIQITLIDLAIEGYPSFFLSFEQDKRKITYRYLPTALINALPNA
                     LLVVVNIIAVYLLGKTFAWGNAETTTLMYYLLIGISCMAVVKACYPFNPLRIFLAVTT
                     IVGIYVAAMLFHGILEVNLGLGESVGYFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(24022..24882)
                     /locus_tag="IXG44_000836"
     CDS             complement(24022..24882)
                     /locus_tag="IXG44_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HAQ1657572.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(24941..25573)
                     /locus_tag="IXG44_000837"
     CDS             complement(24941..25573)
                     /locus_tag="IXG44_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HAQ1657573.1"
                     /translation="MKRKRLSIIRILFYMIILTVIGAVLFLGGRTYRSVKRVESYQIE
                     ITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSESLHGEAEIIDNPADSIDQGVK
                     YLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHGGENTVELAEAYSKDVLSPLLG
                     NEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKMNEKIIETYEKVFK"
     gene            25782..26501
                     /locus_tag="IXG44_000838"
     CDS             25782..26501
                     /locus_tag="IXG44_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ1657574.1"
                     /translation="MKKEKKYAKMPDGSEIYYEKSGQGFPLFLLHGNDGSGRFFSEQV
                     PVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEKIDQADFLGFSDGAN
                     LALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVWLLSLFRPSLRKNLL
                     VIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKTIPKASFVLVKEQGH
                     ELARKDPERFNREVLQFLSET"
     gene            26527..>28835
                     /gene="mprF"
                     /locus_tag="IXG44_000839"
     CDS             26527..>28835
                     /gene="mprF"
                     /locus_tag="IXG44_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAQ1657575.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLG"
BASE COUNT         7929 a         6134 c         4943 g         9829 t
ORIGIN      
        1 aaaaactgag acaaaagcag ctgctttcat ctcagtcaca gatacaaaat caattatttt
       61 tcagaatcat cggttgcttt ttcttctact tcttctttca acgcatcacc taaaatgtct
      121 cccatagtaa atccagtatt ttcttctggt aattcataag actcaacatt ttttggttct
      181 tcttctgaag ctggtttttc ttctaaagct ttgatactca atgcaatacg atgttcttct
      241 ggatggatct ctaatacttt cactttcact tcgtcacctt catgaagcac ttcatgtggt
      301 gtcgcaatat gtttatgtga aatttgagag atatggacta gcccttctac tccagggaac
      361 acttcaacaa aggcaccgaa gcttgtcaaa cgtttaactg ttccgtctaa tactgtgcca
      421 actggagctt ttgtatcgat ttctgaccaa ggtccaggct gtgtgtcttt gattgacaag
      481 gaaacacgtt cttgttcagg attgatagac aacactttga catttacatc atcattgaca
      541 gccaatacat cacttggttt tgctacatga ctatgtgaaa tctctgagac atggactaga
      601 ccgtcaattc cgcctaggtc gacaaatgcg ccaaaatcag taagacgtgc aacacgtcca
      661 ctgagaacat ctccctcatg gatagttgaa agaatttctt ttttcttttc ttctctttcg
      721 ttttccacta ccgctttatg cgataagatc aaacgatttt ctgatggttc gatttcgatg
      781 attttaaagc tcaatgtttt gcctttatag tctgaaaagt ctgcaacaaa atgatcttcc
      841 accattgatg cagggacaaa accacgtaca ccgacgtcaa cgactaaacc gcctttgaca
      901 acatttgtaa caggtgcttc gatgaccttg ccctcttgga aatctctttc gatatcttcc
      961 caaacttttt tagcatctaa acggcgtttt gaaagtagat agctgccgtt ttctttatct
     1021 ttgccgatcg tactgataac gactaaatct aatacatcac cgacttttac tacttcgctg
     1081 atatcttcta ccggcaaagt tgataattct tttgccggaa cgactccttc aactccagcc
     1141 ccttcgattc cgacaatcac ttgtttgtct tcaatgacta aaacttcgcc cttaacaaca
     1201 tcgccaactt ttacttcttg tacgcttttc attgcgtctt ccattgactg gttgttctct
     1261 tgattttgat caaattctgt cataacactt gtcctcctaa ccaacattct gccgtaagta
     1321 cggtactgtt tttagtttaa ttgattattc aaaaaaaata aagcgatttc gttcgttttc
     1381 cttgaaaaaa caattgtttt tcattttttt tcttaattta aaaaagaaga taaccttttt
     1441 tcaaaacaac agcttttatt gctgcaacga cttctggaat cgatttgcca gtcgtatcga
     1501 tacgaacagc atcctctgct tgcttcagcg gagagacttc ccttgttgaa tcataatggt
     1561 ctctttcctg tatctctttc gtcagtgttt caagatcagt cggaattcct ttttcttggt
     1621 tctctttgaa acgtctttct gcacgctcac ttacgcttgc tactaaaaag attttcactt
     1681 ctgcattcgg taatacagct gtcccgatgt ctcttccgtc catgacaacg ccaccgcgtt
     1741 cccctatttt tcgttggaca gcaactaatt cttctctgac ttttccatgt gcagagactt
     1801 cagaaacagc ttttgtgaca tctggttgac ggatttctaa agtcacatct tttgatgcta
     1861 tgaatacatg ctgtccatct tcttgttgcg aaaaagagat aggatacttt ttgatcagcg
     1921 aaactagccc ttcttcatct gaaaaagata cattgttcgt aatagctaaa taagttactg
     1981 cgcgatacat ggcaccggta tctgtataaa tataattcaa ttcttttgct aatatcttgg
     2041 caacggtact tttcccagat gaagcaggcc cgtcgattgc aatactgatt tgtgtcatga
     2101 tataactcct ttttctcaga aaaaaagccg caaagcgcgg ctattattat ttaattctta
     2161 attcttgacc agggtaaagc ataaagttgt ttggatctaa gccattcaat tcaaacaatt
     2221 gatcagtggt gatccctgca cgctcagcca cttgttttgg accttcgcct gcttgaacag
     2281 tcgttgttga accggctgca gctgatgaag aaggtgttgt ttctgctgca ttgctgcttg
     2341 aatcccctgc ttgataagtt gcctcacttg aggaagtcgg tgttgtctct gctgcaatac
     2401 tgctttctga cgttacacct gtatcttgag acacagtaga ttcttccgcg attgtactag
     2461 attctactgt tgaagtctca gttgactcct cttttgttgt acttgtagaa ctttctacta
     2521 ctgatgtcga agtttcagaa gaagttgctg ctgttgcatt gttgcttgat ccatttctga
     2581 tccagaaata agcacctgct ggaatagcga tacataagac cagtaaaatc aaaaatagcg
     2641 aaagaaataa tgtatttcct ttcttttgtt gtctctgaga acttcttgat gaaacttcat
     2701 catctgtatc gtagattggt tgttcccatg gttcttgtgt ttcgttattt tctgtagatt
     2761 gatgtctatc ttttctgctc actgttttac ctcctaaact ctatcgtgtg tattatacca
     2821 taggaaaaag tgtcttgtcc taaaaaatca atgttccatg ttttttttaa ataaatttag
     2881 taaaatcgtt tcccaatgaa gttgttcagt cttagaaaaa agcttcctct ttggcagaag
     2941 ttctgctttt gctccatcaa tatcacaaca attctgcggc ttttcggaga gtttttcgcc
     3001 aaaataagct aagatgaatt ttctgcggca gttttcttca ttaatatagc gaagcatctg
     3061 ctgcaggcgg aattcttttt ctttttcctg acgtttgagc cgttccaccc aattttctgg
     3121 tttttcagac tgctggatca gctccatcca ttttttttgc agctcatcaa agggagcttg
     3181 ctctgtggca tattccaaat aaagctcaaa actttgtcgc tgttctctag aaagttgatt
     3241 aaagaaataa tggatacgtt catccccact ttgataaaaa agaattgctg cactttcttc
     3301 ctgatctctg cctgctcgac cgatttcttg tacatagttt tctagactgt ctggcaaatc
     3361 ataatggacc acataacgga tatcagattt atcgatcccc ataccaaatg cattcgtagc
     3421 aatgagaaac tgcagctgat tttttacaaa ctgctgttga agttgtcttc tttgagctgc
     3481 atcaagacca ccgtgatagt atccaactgt aaaacgttcc ctgaataaat gatacaatcg
     3541 ttcgacttct ttttttgttg cacaataaat gattgccgct ccttgtgctc tttccatgaa
     3601 gtgttcaagt tcttgttctt tttgctgcgt tttccgaaca aaaagcgaga tattttgccg
     3661 attgactgat tgtctgatct cttttggtgg tcgatcaaat agtacatgac gtatatctct
     3721 tgctactaaa tctgttgctg tcgccgttag tgccagtgtg accgggtttc ctaaaagctt
     3781 tcggattttt cctaactgtt gatattctgg acggaaatca actccccatt gagacacgca
     3841 atgagcctca tctactacat ataacgcaat attttgtttt ttcagatgct ctaaaagaga
     3901 aggattagtc aacatttcgg gactcaaaaa caaaaattta tattgcgata gatgacggag
     3961 gacgtatctt ctttcagatt gcgaaagtaa actattgaaa gcagctactc gtttctcccc
     4021 tcgtttttgt aattgcgtca cttgatcttc cattagagaa agcagcggtg acacgatgat
     4081 gaccagacct tccattaaat accccgtcaa ttgatagcaa aggctttttc ccgttcctgt
     4141 aggcaaaata gcgagagtat cttctccatc aagcaacgcc tgaataattt cttcttggcc
     4201 gggacgaaat gaatcaaatc caaaatattt ttttaattca tttattaaca ttctgtatcg
     4261 cttcctttct caatagatag atttgataca aacgaaattc tccataatct aattgtccat
     4321 ctacattcca ttcgctaaag cgccactctc gcacggaatc cttatttggt tccaaacgtt
     4381 cgattgtttc tgcagagaga atccactcgt atggaaaatc atcaaaaaac aaggcccact
     4441 caataagatg atctgttact gttcctcttt ttaaatgacg gattgccatg atctggtcaa
     4501 ttgtctgacc attcataaac atctgcttgg tgatcatcat gctttgattg aaattttgct
     4561 gtaataatga atcgatcaaa gcgtgccaaa gcgaatctgg ccgtttttcg atttgtgcta
     4621 acaaaaggtg gatacatctg ctttgttgca gataaaccgc tgactcatca ttgttttttg
     4681 ccagctgata aggaagcagc cctgttcgct catttccaga taattggtta gctaaaaaat
     4741 ctgctgcttc ttctggtatc tccgaaaaca gctgcaccaa actctcatac gcagtatcaa
     4801 tcaaaatttc acgtggcaat tggctttcac taagccattt ttttagttgg aaagtataaa
     4861 aaggacttgt ctctgctgga agatagtctt gaatacccgt tgccagatta gaaattactt
     4921 gaacagcaaa tttaatgagc cgccaactag tttcccctgt tctgccatag cggtcaaaac
     4981 gcaaaccagt atgttctatt tgatgttcgg acaacatatc agcccctgca ggcgttagct
     5041 ttgcttcatt ttcatttatt tcgatccaac cttgctgcgc aattttttgc aaaatttgat
     5101 aaaacttatc ctgcttcaat tctggaaaac tgttatggat aaacaaaagt tcgtgggtaa
     5161 atccataaat aagtactgaa gtcgttcgtt ttcccacaag gagttggtaa agagaactaa
     5221 tccgcagctt gtgtcctgat gaaaatagcg ataaaataaa aacaatcata ttattctcct
     5281 taagttagga tggttagaaa tgtcactact atgtaaactt gtacctgaac gctgtattgc
     5341 atgcggtctt tgtcaaatcg aagcgccaga tattttcgat tatgatgaag aaggaatcgt
     5401 tttatttgcc caagaaccag aggctcatca acagttcgtg atgccggaag aagaaacata
     5461 tgtaaaaaaa gcgtatcaaa aatgtccagt tcgagcgatt ttattagaaa ataaaatata
     5521 aatataaacg aaactttaaa aacgtacact aagttctcta aacttgaaaa taagccggga
     5581 tctgtttctc ttgttccgtg aaatcccgac ttattttgaa agattcattt gctatctata
     5641 gttaattgac tagttttttc acggacccta taggacccat ttatattatt tcaacttttt
     5701 cagccagttt tccaatgtat tgacgatcac agtttgctgg tctgcgttag aaatttctgc
     5761 ctctccatct cctttttgct caccatagct gccaaaacct ccatgatttc cgccattgat
     5821 tgatacatac tctgttcttt ccggtaaata ctgcttagct tcttgataag cttcctgatt
     5881 caatacttta tcttttgtcg ccgtgatcga taaaacagga acagctgatt ctgtcaatgt
     5941 tccttttttg tcgggataac ttgccatgaa gaatacccct tttaaatgtt tgtctgaatg
     6001 attcttattg acataacgac ttgccattac gccacctaat gaatggccac cgatcacata
     6061 atcggaattt ggaaaatctt ctttgacctt atccgctttg tttccgccca tcactgctag
     6121 atcaagtggc attttgacaa tagcgactga aaagccgttt tttgctaaat cttgcgccca
     6181 aatactataa ctttctggct ctacgagagc accaggataa aaaataagta atggagactt
     6241 tccatgattc tttttctctc cttcaaaaaa taagtaatct tctttgtttt ttccttcttt
     6301 taccaacact tgcgcttctg acgatggttc gtacgtattt tgctgtatat agtaataacc
     6361 ggctccaccg agtaatccta aaatgatgac taagccaatt agtattcgtc taaatatttt
     6421 cactttctcc caccttttct atctgagtga cccctttttc ttaatcaagt atgattatac
     6481 cttaaaaaaa taagagagaa atgcaaactt tccagcatca aataaaaaaa gacggaatat
     6541 ccatttgaat atccgccctt tttgtatgtt tatttttatt tagcacataa acgatacaca
     6601 gcttctgcat aaatttccat tgctttatac atactactta attcccatcg ttcattcgcc
     6661 tggtgcataa agtcaggagt atcagggaac atcgcaccga atgccacaca ctggttcatc
     6721 gtacgagcaa aagtagcgcc gcctgataca aacggttcag tcatgtctcc agtaatatcg
     6781 cgatatgtcc ccaatagatt tttcactaac tcactgtcta gtggcacgta taatgaatca
     6841 aggaagtcaa attcttcata agtcaaccca tatttttctg ctgttttagt taatttagct
     6901 actaaatcat ctttcttgaa tgtaacaggg attctcatat ctacaccgat ttttgtttgt
     6961 tctggtgtga tttccaaact tgcaaagttc atcgttagct cgccggattg ttcatcttct
     7021 gttttgccca cgacagcttc accagttgca ttttcttgaa ctaattttcc taagaaattg
     7081 attgggccaa aatcaaatac ttctgaaaga gcaattgcca gacgagaaat agcattcact
     7141 ccttgtgctg catctttcgc gtggatactt ttaccaagaa cgacgatgcc ttcaccttgt
     7201 tcttcgaaat cgaattcgtg ttttttcaat gcttctttta cttcagaaag cttttctcca
     7261 gagtaaggag ctgcatcagg aacaacattc aacgctcctc cagcttttac agaaaactcg
     7321 ttcgttcctg gtcctgtcaa gtaagcttgc aataagcctt tttctgcata aattaaagga
     7381 aattcagcat ctggcgcaaa tccttgcgta attccttctt ctttttcgtt atatttttct
     7441 aaacaacgcc ataaattttc ttcatctgta ccaaagataa aacgaatacg tgtcttgaat
     7501 tccacgccag catcaatcaa agctttgacg gcatacattg ccgcaataga aggacctttg
     7561 tcatcttggg atccgcgacc aactaaccag ccgtctttta tagttggatc gaatggtttt
     7621 gtttcccaat tattttcgtc ccctgctgga acaacgtcca tatgacacaa gataccgaat
     7681 aattcatctc cagagccaat ctctgaataa ccgtaatagc cttctggatc tttaaatgtt
     7741 ctaaatccta gcttttcact gatttccaat actttatcca aagcttcaat cactttttta
     7801 ccgaagggat gtccttgtcc tgaatctgct tcatctagta cggaaggaat tcggatcaat
     7861 tcgctcaatg attctaatgc ttgttcatga tgttgttctg tcacaaattt tttcatgata
     7921 ttatcctcct aatatgttaa aaagggtcat gtcaaattcc tccgacacaa cctcttcttc
     7981 tcaaataatg acgttttaag cctgctccat cagcaagcag aatgttctta acaaatgatg
     8041 aaagaaaaaa cactcactga atggagctat tgacttatcc acgtgcttat cgatagccgt
     8101 ttatcggttt cttcattctt atgatagacc ttgtacctgt atttgtctag gtttagaaca
     8161 ttgctgctac acctaggatc acacagatgg ctacaaataa tatcgcaatt aatttaccgg
     8221 taaatttcca ccacactgaa acatcgaaac gagcaattgc tacagcaccc attacgatag
     8281 cagatgttgg tgtaattaag tttactaaac cagatgcaga ttgataagca gtgattacta
     8341 aatccttacc gacgccggca aattcaccca taggtcccat gattcccatt gttgcagcag
     8401 ctaagccgga agttgaagga atcaagaatg acatagggat atagaaaata aatgtaataa
     8461 tgatgaatac acttgaagat aatgaactta atccttgttc gccccaatgt aagatggtgt
     8521 ctgtgattaa tccattgttc ataaccactt ggataccacg agcaacagct actacgattg
     8581 ctacaccgat caaatcagaa gcaccagcta aaaattcaga gacaaattca gactctttca
     8641 tgccataagt gattgctact acgatggaca ttactaagaa taacatagta atttctggga
     8701 agtaccagtc acctaatgga agcatgcttt tacctagaac cgtacctagt accggaaggt
     8761 ttgttaacca gtttgtcaat gattcaaaaa tcgtgaaatt cgcattcaag gttgtccaag
     8821 gaatcaaact gatgatcatg ataccaaaag taataaagaa caatacatta acatgttttt
     8881 gttttttcgt gattacttct tgacgaccga ttgcttcaat atcgaaatgc ttcatatctt
     8941 ctttacgttg gttgtatact aatgattttg ttggatcttt ttcgatcttt gatgcataac
     9001 gatacacata tacaatagaa atagctaaca aaataacgaa catcaagaat cgaagaccaa
     9061 taccttcacc catcgaaatc ccaacagctt gtgaagctac gcctgttgca aaaggattta
     9121 ccgttgaagc tagacagcct acttgagacc ctactaatac gattgctaca gctactactg
     9181 tatcaaagcc tacagccatc atgactggga tcagtaaagc atagaatgga atcgtttctt
     9241 ctgccattcc gtaagtagag ccgcctaatg caaataacag catcaatact ggaataagca
     9301 ttttttcttt tcctttgaat ttcttcacaa ctgaagcaat cccggcatct agtgcccctg
     9361 ttttgttgat aatacctaaa aatccaccaa caaccaaaat gaagaaagag attgggattg
     9421 cagcaggtgt ttctttagtt agctcccccg ctggtacacc tagcatccca ttgattggcg
     9481 ccatgaaaat atcccacagc ccttgaggat tttgttgtac tcgttcatat gatccagcga
     9541 cgatgttgcc tgcatcgtct acactatagt gcccagctgg tataaaccaa gtaagtacag
     9601 cgattactgc aataataata aataagaccg tatacgctga aggcatttga aattttttct
     9661 tttttgtttg ttccatcttc ttcttccttt cttttccgtt agagacaaca attttggctc
     9721 taatgttatt ttatcaaaaa gagtatcaga acaaaaataa taacacgcat tttgtaaaac
     9781 ttttgttcaa aaagttcgtt tttccctatt ccctcctctt ttttcaggtt tttaaagctt
     9841 tcttcttttt cattgtagcg tttccttttt tttataaaca aacattttcg tcttttaaaa
     9901 ggagaagaat ttcacatttg caaagaattg attgattttt atgcataaat caaaaaaata
     9961 ctcaaaacaa acaaataaaa cgtttacatt attgcatgtc ttgtattttt ttccaaaaaa
    10021 aaagaccaag aaatttcttg atctttttga tatttatttg actgctgtat gacgttgttc
    10081 gaactgatgt tgttttcgac ttaaccatgg aagaagttgc gcatgcagca ctaaaaagac
    10141 agcactcacg atgaatcctt tgatcaagtt gaaaggaacg atcccaatca gtacatagtt
    10201 tgctaaagac atgcccagca tctgtgttgc gttcagccct aaaaacatta agtaaagagg
    10261 cgtaataacg aaataatttg cgatactcat aaaaatagtt agagcgatgg ttcctgtaat
    10321 gacacctaaa aatttgtttc ttcctttatg ctgtttcttt acactaaaga aataataaat
    10381 cgggaatgta aagatcgttg ttgataagaa actagcagtg tctcctacca aattatccgg
    10441 agaaaaacct gtagtcagca gatgtaatac tgaacgcaag aacgctgttg caactccggc
    10501 cgctggaccg aataagaaca tgctgatcaa taccgagata tcactaaaat cgatcttcaa
    10561 aaaactaaaa gccggcatga tcggaaaagc aaaatactgt aaaactaatg cgatcgcagc
    10621 aaacatcgct actgcaacca ttttctgtac acgggtgttc ttcatttgaa atcctcctgt
    10681 taggactcct cttcagcgaa ctttttgacg tttctctcca aatgaaaaaa ctctattttc
    10741 cagggaaaat agagttagag ttatttgatc aagcgtcgtc aaataagctc tatcttcttt
    10801 atccagacta tactgtcggt atcggaattt caccgattca gctatttttt tgcaaaatag
    10861 gtcgtggact ataaccaccg gtcgggaatt tcaccctgcc cctgaagacg aaccttatat
    10921 aatttttgtt tacatattca ttatacatat ctattcaaaa aacgcaacat ctaacactta
    10981 taaaaagtaa gtgttcattt tgagtggttc agcaaactgc tgacttcttt tttgttcagt
    11041 tcacggtaat ccccaggacg taggccttgt aaagtcagat ctccgtattt ttcacgtttt
    11101 agtttttgca caggaagacc aactgcttga agcatatttt tcacttgatg attacgtcct
    11161 tcgtggatcg tcaattccac gatactgtgg tttgtcttag gatccactga taaaatttgg
    11221 aatttagctg gagatgtttt tcgtccttcg atctttattc cttttgtcaa aggtgcaagc
    11281 atcgattttg tagcgatccc ttttaccttt gcaacgtaaa ctttatccac ttcatgtttt
    11341 ggatgtgtca ggcgctgagc aaaatctcca tcatttgtca gtagcaagat ccccgaagta
    11401 tcgtaatcca gtcgacctac aggatagata cgttctttta ccatcggcaa aagatccgta
    11461 acaacttttc ttcctttgtc atctgcaaca gcagaaataa ctcctttagg tttgtacaat
    11521 aaatagtaac cgtattcttc ttgataaatt gggacttcat ccacttccac agtgtcatgt
    11581 ttcccaactt ttatccctaa ttcccttaca acttgtccgt ttacttttac acgtcctgca
    11641 aggatgaatt cttctgcttt tcgacgagaa gtgatgccag cgtgggcaat tactttctgc
    11701 aatctctcca ttacaattat tctcctttat tcattccatc aaagaataga tctggtgaca
    11761 cgtcttcttc aagtgaaatt tccatttctt gtgtatcagg cagttcttct aatgatttca
    11821 aaccaaaata atctaagaag tagccagttg ttccatataa tatcggccga cctggtcctt
    11881 ctactctacc tttttcttca atcagtttat gggcagctag acgctgtacc gctcctgaaa
    11941 cctgaactcc tcgaatctgc tcgatttcag ctcttgtgat tggctgttta taagcgatga
    12001 ttgctaaggt ttcgacggcg gcttgagata atgtgtttga tgtcccttgc gcatatcgct
    12061 taagtaaggg agccagctct tttttagtcg ttaatacata atggctttcc gtttcaaaaa
    12121 tatgcaaagc tgagtgcgag ttttgagtgt aagattcttt taattctttc actaaacttg
    12181 tgacttctgc ttcgctgaga tttaacagtc cgcttaattc ttcattactg atcccttcct
    12241 cgccaacaat aaaaagaagg gcttcaagtt cacttaattt cgtcatgttc ggtatctcct
    12301 actgctggat aaagcataat tgattcatag ttttcttttt gttgcgcaat gatctgccct
    12361 tttttcatca attctagcaa agccataaaa gtagtgacaa cttcactttt agaaggtgac
    12421 tcaaaaaaag aatcgaaagt acagccatgc gcatttcttt ttatacgctc ttcgataaat
    12481 accattcgtt cttcgatcgt tgtgtcatcg ggcgtgatcg ttttttccac tttttgagaa
    12541 aatttcttac gttctaagat tttctgcatg gcacggatca aatcgatttt tttgacttgt
    12601 cctcgcttca acggcttttc agcatccgct aacccatcga tgttcatagg ttcttttgtg
    12661 taataaagac ttcgttcctc tgctttttct gtcagtcttt cagctgcata tttatattta
    12721 cggtattcta aaagctgagt gaccaattct tctcgaggat cttcttccca taattcttca
    12781 tcgtctgtga cgaattcttg gacaggaagc aatgtcttgc ttttgattgc catcaatgta
    12841 gcagccatca ctaagtattc tcctgcaaca gctagctcga gtgttttcat tgtatggata
    12901 taattcatat attgctccgt gatcgtagcg atcggtatat catatatatc gatttccatt
    12961 tgctgaatca gatgaagcag cagatcgaga ggaccctcga atacatctaa tttaatattg
    13021 atctccgtca actcattcac tctttcttct ttttctttgc tagccatttt ccaatgtaag
    13081 gctctgtgat ttcagttgca cggatggaga catccgggta cttttcctgt aattcatcta
    13141 acatttgaaa gcccatattc tcaccgcgat aagacggatt caaagaaatg tcatggatga
    13201 tcagctgatc gttcgttgcc cagtaaatac caataatccc ttgaatgttg gttgaacctt
    13261 ctggcgtaaa gaaaaaaagt tcataatcgt cttcagattc gtacgtatca atctccttca
    13321 acaaagaagt tttacctgtc agtttcttat ggaaactcaa tagtcccata gcaattttac
    13381 ggttttcttt ccggtaatgt gttaacatct tctcaccttc ctatgactaa gcccttggaa
    13441 aatgctgctt ataaacatcc gtcattcttt tcttcgtgat atgtgtataa atctgagtcg
    13501 tagagatatc cgcgtgacct aacaattcct gtactgtccg caaatcagct ccattttcta
    13561 gcagatgtgt ggcaaagcta tgccgcagcg tatgtggtgt tacttctttg taaatcccag
    13621 cctctcgtac taactgcttc agatttttcc agattccttg ccgagaaaga cctttcccat
    13681 gattattgac aaatacatgc atttcagatg cgtcctttac aagtaatgga cgtgcttcgt
    13741 ctaagtagcg ttccagccat tggatcgcat aatcacctaa aggaatgatc cgctctttat
    13801 ctccttttcc aactgtctgt accagtccaa gagaaagatg aagatctccc aatttcaatc
    13861 caatcaattc actgacgcgc atcccagtag catacatgac ttccaagatt gcacgatctc
    13921 ttatccctaa cgtttttgtc gtatcaggtg cttcaatcag cttttctact tctgtcagag
    13981 tcaatgtact tggtaatttc tgtgctttct tcggcgtatc gatatgttgc atcggatcat
    14041 gatcagtgat acgctcttgc cgtaaaaact gatggaatct tctgagactt gagatcatcc
    14101 gagtaacagt tgctgtcgct tttttttctt catgcaactt ctccagaaaa cggatgacta
    14161 aaaagcgatc gacatcctgc catgaagcaa ttttctgctc ctgtagaaaa tgcaagtact
    14221 gttccaaatc acgttcatag ctttgtcgtg tattggcgga caatccccgt tcaatattca
    14281 agtagtggag ataatcgttg atttgctctt tcataataat ccccctcatg cctctctatc
    14341 atatcagata ccgcttcatt ctgatagtct ttttccataa tctgagcaaa gaaaaagccg
    14401 tctagacact cagacggact ggtttctttt ttctttttca tagcattcct gacagattcc
    14461 atggaacgtt agccgatgat cttttactaa aaaatgataa cgttcttcta ctacttgctc
    14521 cacttcaagt aaaagatcct cttctacttc ctcgatcgtg ccgcagttca agcatagtaa
    14581 atgatgatgg aaatgtttgg cgccttcttt tctcaaatca taacgggcta atccatcatt
    14641 aaaacttact ttatcgacaa ttttcaattc tgtcaagatt tcaagggttc gataaacagt
    14701 agcaagtcca atttccggac tctttttttt cactaaaaag aagatttctt ctgcagataa
    14761 atgctctttc tcattttcca aaagcacaag cagagtagct tctctttgcg gtgtcagctt
    14821 aaaccccgct tcatgtagct gctgttttgt ttttttcatc gccgaaatag cattcatgac
    14881 catttactca accccaacaa tttataataa ttacaaataa aaaaagatat aacactatgt
    14941 taattaaaat tattataagc tagttaagaa aaacatgcaa gggattctca attagatttt
    15001 ttgactagtt ttgtcacacc gttttcttgt gtaatcaatc cttgtttcaa aagattacct
    15061 aatgcgcgct taaatgctcc tttgctgata ccaaaagctt tcatgatttc ttctggatcc
    15121 gatttatctg taaagttgat ttgctgatct gcagaacgtt ctaaaatggt taaaatcatt
    15181 tgtgcatcat ctgatatagc ttcatatgct cttggcttca atgaaagatt cagtacaccg
    15241 tctggtcgga caccaatcac tcgacctttt atctgctcgc ctagacgagg ttcattatat
    15301 cgttcagaag gatggataaa cccaagatag aaatcttccg ttaaaacgta cgtacctgtt
    15361 aatttcaagc ggtatgctgt tcctatcaca tctttgtttt tcatttcttc tgttccatat
    15421 ttgcttaaag atttgaatat tttttcatct gctaaactgc cccaaatacg gtcttttgca
    15481 tcaactctaa gggaaatcat caagcgatcc ccttttttag gccatagctc tgtcattgtc
    15541 ggcaattcat caagagatac gacaaagtct ttatctggaa gaccgatatc gacaaacgca
    15601 cctaaatctt ttcgtgtagc agtaacgatt ccaaaagcgt aatgaccgat acggctttta
    15661 ggaatttgtg tagtgatccg attttcatgt ttttgatttt ggtaaccgaa tccttcaact
    15721 gcttcaccga ttttgtgttc accttctgat ttgcttaagc caaaggtttg tccgtttttt
    15781 tggacaaagt agtttttttc attttcatca ataataatac ctgtaaatat agttgctaat
    15841 aaatcgttca tttttttctc cctctatagt gaaaggtttt cttaatacat ttctttgcta
    15901 tcataacata atgttatcta cttgaatagc tgagccttgg tttggaaagg tgaaaaaagg
    15961 agtatgacaa cagttgtgtc atactcccct ttttcgaata aacggtgtta aacggcaggc
    16021 tcacagcctc tttcatttgc ctaaaattaa ttgctcccat ttcctccgaa ccatccagtt
    16081 ttacagttga tggttacgat gtaaaatggc aaagcacttt ctccaatgcc tttcatcaat
    16141 gcagattttg ttgaagtggc atatagatta taatagcctt ccccattttc gattctggct
    16201 ttttctaatc tgtaacctcc ttcaacgata tatccacgtt ggatacatgt agcaatcact
    16261 ttgctttgga cacctggtgc ccacgtccat gctggattag atgcatcggc aatatcactt
    16321 gctctggcag atttgattgg ctgattcgct gagtttgaag aggaaggggt cgaggatgcc
    16381 gcttcttgct gtgctgcttg tgcgaccgca gcttgttgag cggcttgagc tgcagctgct
    16441 gcttgagcgg cttgttttgc ttgttcttca gctgcttttt tcgcttcttc tgcttttttc
    16501 ttttcctctt cttctttttt ctctttatca gtgagcatct tatctacttg tttcaattgt
    16561 tcattcaact gagcagatag atctgtattt ttcacttttt ctactgcttc ttttgccgtc
    16621 ttgtattggt cgcgtgttgc tgaatccact acttttccgt ctttaaagac tatagctgtt
    16681 ttttctttag ctgtttgcaa ttcagcatat tgatttttag cttctgtttg tgcttgttga
    16741 atcaattttc caaaatctgt gttatccgtt tcgattgctt ggatctcttt atctgctttc
    16801 aacttgatgt catctgctag atgatcatcg acgatggcag gtgctatgaa ccactcattc
    16861 actttttgga tatctcggat cttagattgg atatcttggt aagttgtttc taactctgaa
    16921 tatccttttt cattcttaag actggataaa tcatttttta gttttgataa atcttgatga
    16981 atcatatctg cacgaataaa ttgatggtga tcatctgtat aaaaggcagc aagtttctca
    17041 tgaagttctt ccatttgttg ctgtcttttt gcttctgctg ctacttcggc ttctactttt
    17101 tgatgatggt cataataagc ccagcttcct ccaccgagta gaagtactgc tgctaaaacg
    17161 agataaggta ctttttttga ttttttcttt tgcgctttat ctggatctgt tttttctaag
    17221 tttgattctt gatgtgtttt ttcagtatct ggcacgattg ttattgattt cgcttctgct
    17281 tcaaacagct taggctggtt ctcttctttt tctatttttg cttcaagatc agtaccctct
    17341 ttgcttacag attgattttc agcatcggat tcactattta tttcatcggg ctctgttgac
    17401 tttttgtctg tgttttcgag tatttctttt ttctctgctt ccggcagttt attcgttttt
    17461 tccttttcta attgtttttc agtaaatact tcagtttttt ctgtgatatc cgtttttacg
    17521 tcttttattt ttactgtttc tttacctgtg gcgttttctc tatcagagtc tttttcagtt
    17581 tcttgttcct tttctggctc tactttgttt tcctgtgcaa ttttttcact gatcattttg
    17641 agcagctctt tcgcgtgctc ttcttttgta tcttcacgat gtgctttgat ataagcggat
    17701 agtatcgaat tttcttcctc attatcgacg atttctttca gttcagctac tcttcttttt
    17761 tgttttgatt caagcgtttc ttcttgattc tttcctgatt cttccgaagt ctcatgaaca
    17821 gttgctttct tctgttcatt tttctctata ttagttactt gatcgtgagc aggtggttct
    17881 tcgtcttctg gttctgcttt ttcgaataat tccattacag attctagagg taaatctttc
    17941 aattccgacc attcgattgg atcgtttaat tctgtatcag gaaagactgc atcttcagtt
    18001 gatttatcct gcatagctgc atctgtattt gtttcttcaa ttgattcttt tatttgctga
    18061 tcttctgcag ttatttgatg accgcagtct ggacagatag tattctgctc catttttttc
    18121 gatccgcaat ttggacattt tttcatcact tagcgatcgg ctcctttact aggtttacgc
    18181 taccatttaa tctatcatat tctatacgtc acgccaatag cttattttta ggatttactt
    18241 aaaaataatg aatgaatttc cttcctatag aaaaaaaagc ttgagactgc gtagtctcaa
    18301 gctttttttc ttttaaaata aaccaaacag ttttactgat ttctcttctg gtcaatagtc
    18361 cacgcttatt ctaagaaatc ttttaattgt tttgaacgag aaggatggcg caattttctt
    18421 aatgctttag cctcgatctg acgaatacgt tcccgagtaa caccaaatac tttccctact
    18481 tcttccaatg ttctcgtccg gccatcatct aaaccaaaac gtaagcgaag gacattctct
    18541 tcccgatcag tcaatgtatc tagtacatct tccaattgtt cttttagtaa ctcataagct
    18601 gcgtgttctg ctggacttgt tgcttcctgg tcttctatga aatcacctaa gtgagagtca
    18661 tcttcttcac cgattggcgt ttcaagggaa acaggttctt gcgcgatttt caagatttct
    18721 cggacttttt ctgttggaag atccatttcc gcaccgattt cttctggcgt aggttctctt
    18781 ccaaggtctt gcagcaattg acgttggata cgtatcaatt tattaattgt ttcaaccata
    18841 tgaactggaa tacggatcgt acgtgcttgg tcagcgattg ctcgagtgat ggcttgacga
    18901 atccaccatg tagcataggt tgaaaactta aatcctttgc ggtagtcaaa tttttcaact
    18961 gccttcatca gtcccatgtt tccttcttgg atcaagtcaa ggaactgcat gcctcgtcct
    19021 acataacgtt tagcgatact tactactaaa cgaaggtttg cttctgccag acgctgtttc
    19081 gcttcttggt cgccttcttc gattttcaat gctaacgcta cttcttcttc cgcagttaaa
    19141 agggagacac ggccaatttc tttcagatac atacgaaccg ggtcattgat ttttacaccg
    19201 gttggcgcag aaagatcttc tgtttttgct tgttcagctt ttttctcagc actcttcaag
    19261 ctgtgaattg atggatcacc attttcatcc accacactga tacctgcgtc ttcaatcttt
    19321 tggatcaatt tgtccatttc atctgcattc aaagtgaatg gtgtagcaag ctgattggat
    19381 aaatcatcat atacaacttg tcgtttgggt ttgttttctt tgatgaaagc tgctactgct
    19441 gcttcatacg tttttttatc tgttccattt gccataaaag aaggcccccc ttgagtagct
    19501 atttggcttg ttttaactgt ttcgttaaat tgatgatttc aatcgtcaat tcaagctcaa
    19561 gctgtttgtt tccagttcgt ctggcttctt gctgcatttg tttttttaat gttatttcat
    19621 cagctaagcc agatttgctg atcacatcta gcaagtcttg gatttctcgt ggcgaacttt
    19681 cttctgccac cggtaaatat tcgatttcca ccaccaaccg ccttaaattt tcatcttgca
    19741 aaaagtctaa aaactctgca aggttgaatt cataatgttg tgagacatat gcatcgaata
    19801 aaaaataaac ttctgcatat gcatcatgaa taaactgaaa ttctcgttct tttaaaatat
    19861 tacggacaga aacttcttta aatgcacgat ataatagaat ctgctctgcc ttttctactt
    19921 gcgtcaaagg tctctttttc cgtgcagttt cttgtctcgt aacctgagtg tgttgacttt
    19981 cctgaggttc ttgacgattt tgctgatttg tccttcgaag atcgcgcagt tgtttttgta
    20041 tcgtctctct gccgatctga aactctgttg acagttgaga tagatacata tcttgttcga
    20101 ttggtgaaac aactcggcta agttcaacta gtaattcttc gaggtattcg atcttgtctt
    20161 tttcattatc taaatttttc ccttgtttta aataacgtga tttaaaagta aagactgttt
    20221 cgcgcccatg agccactaat tcttggaaag atgcttctcc atacttacgg agatattcat
    20281 caggatcaag cttttctgga acgactacga cagaaagtgc taatcgactg ttttgccgaa
    20341 gcaattcaat ccctcgattg gttgcttcaa aacctgcgtt gtctccgtca tagcaaaaga
    20401 ccaattcttt cgtcagcttt tccatttgtt ggatctgttg agctgttaga cttgtcccca
    20461 tagaagctaa tccagttttg ataccagcct gccatgcagc aatcacatcc ataaaccctt
    20521 cgaacaacaa aatttctccg tttttccgga tatcgcttct tgctttatca agattaaata
    20581 aaacttgtct tttattgaac agttctgttt ctggactatt caagtatttg ggctgatctt
    20641 tttcctgccc tttttcaggt gcaagccagc gtcctgaaaa accgatcgtc tggccttgag
    20701 aattacgaag tggaaacatc actctttgat aaaaacgatc agacatacgg ccatcatcat
    20761 gcgtgacaaa caatccagag cgttctaaca attcatttga aattgattca ttcttgaata
    20821 cttgctgaag aaaagaacgc tcattaggtg caaaacctat gttgaattct tcaattaatt
    20881 ctaaagacaa cccacgactt tgcaaatatt ctaaagctgg ctgtccagct tttgtatgca
    20941 atagcatgtg atgatagatt tccgctgctt tttcatgagc agcaatcagt ttccccgtag
    21001 cagattcagg attttggaca gctatagtac gctgacgcca ttgatcttct aacggtatct
    21061 gttcgaagtc tgccactttt accactgctt ctggaaaact tagaccttct agttcttgaa
    21121 gaaaagtaaa cacgtttccg cctttaccac aaccaaagca atgaaaaatc tgcttgtctt
    21181 ctgcaacaga aaaagacggt gtacgttctt catgaaaagg acacagcccc aagtaatttt
    21241 taccagactt tttcaactgg acatattgtc caatcacctc aacgatattc gtttgactgc
    21301 gtactgtttc gataacctct tgaggaatcc gttgagccat tttttcaccc cctgctctat
    21361 tgatatcttt tgggtttaac agaagatatt tcaggagaat aaccggtaaa aaatcgcact
    21421 aactgaaatc agtgcgattt taaaggacac aattatacat gtttgattgt atcatatttc
    21481 attgcttttt caagtataac acatttctat ctcattaagc atcaatttgc tactcttttc
    21541 agtaactgat tttgaatatc agccttccag tacaatactc gaagaacaat atttacgagc
    21601 atgaatacaa agaagtatcc tacgctctct cccaacccta agtttacttc caaaattcca
    21661 tggaacagca ttgcagctac atatatccct acgatcgtcg ttactgctag gaagatgcgc
    21721 aatgggttga agggataaca agcttttaca actgccatgc aactgatccc aatcagcaag
    21781 taatacatca aagtagtagt ttcagcattt ccccatgcga atgtttttcc aagtagatac
    21841 accgcaatga tattcactac tacaagcaag gcatttggta aagcattaat aagtgctgta
    21901 ggcaaataac ggtaagtgat ttttctttta tcttgttcaa aagataaaaa gaaggaagga
    21961 tacccttcaa tcgctaaatc aatcagcgtg atttggatag ggataaacgg aaatccaatt
    22021 gctgtaacgg cacatataat cgataaaata aatgaataaa tcgttttgat aaaaaagatg
    22081 cctgagactt tcgtcacatt gttgaccact cggcgtcctt caaacaggac ttctggcaat
    22141 gtagtaaaat cagagtccaa caataccaga ttcgcaatct gacgtgttgc tccatcacct
    22201 tctgccatag caatgctgca atctgcttct cttaacgcca aaacatcatt cacaccgtcg
    22261 cctgtcattg caaccgttct gccgctttct ttcaattcat ttaccagcaa tttcttttgc
    22321 tgcggactca ctcgtccaaa aaccgtatag cgatgagcag cttctcgcac ttccatttct
    22381 tcagtaattt gagaaagatc gacatacgca tcataattcg gtaatcctgc acgtctagcg
    22441 atattggaga cagtaaaagg attgtctcca gaaatcactt tcagatccac accttcctca
    22501 tgaagataag ctagtgtttc ttgagcgttt tttcggattg gatcatcgat ttcaaacaga
    22561 gcaaccggct caacaaatgc tggtttttct tcgttcaatt gctgatttgg agcgatcccc
    22621 aacataagta cgcgatagcc attcgcttgg gctgttttta gactgtccgg aagccgatct
    22681 tcttccacca aacgttcagg cgctcctacg tacacgactc ccagttctgg aaattccatc
    22741 gcgccccatt tgcgatcaga agaaaatgcg actttgtttg tcacatcata ccgattagaa
    22801 agctcaaaat aatctctcat agcccgcata gtgatgttgt tatcactgct ttctgccaga
    22861 tacgtcccca taatttctgg gatggctttc tcgtacagcg gatgtagtgc ttctacccgc
    22921 tggatactca ttttcccttc agtaatggtc cctgttttat ccaagcataa tgtatcgaca
    22981 tgggccagcg tttcaattga atacatgtct tgaacaagga ttcgtttttt tgccagtttg
    23041 gttacccctg tcgttaatgc aatacttatt agcaatacca aaccctttgg aagcatgcct
    23101 aacaaagctg ctgcagatgc aataacagaa attttgatag aagcatcgcg gatgaacagt
    23161 gcttccaaaa atagtatgat ccccaaagga ataatcacga aactagtgaa tttggatact
    23221 ttgcgaatcg aatctacaag ttcagaacga atagctttgt gagtctttgc ctcgctagtc
    23281 aagcggacag cataattatc tgcacctact cggataactt cagccatgac agaaccgctt
    23341 gtcaaaaagc ttccagaaag caattcagct ccagtttctt tgacaatcaa atcagattcg
    23401 cctgtcaaaa gagcttcatt tgcttctgcc cgtccagata taacctgtaa atcggctggt
    23461 atctgttctc ctgctccgat ttttaccaaa tctcctaata ccagctcttt agtagaaatc
    23521 gattgttctt taccgtcccg gatcactagt acttctgatt gggaaacaat cgataatttc
    23581 cgtacaagat ttcttgcatg tatttcttga taaattccaa taacgatatt gacgacaata
    23641 attgctaaat acgccatgtt ggaatacgca cctacaaata acagacaaat cccaatacca
    23701 aagttcagaa aattgaataa cgtcaatata ttatcgctga atatttgttt tgtacttttt
    23761 gatgcatctt cctggtagtc attttgctgg ccagaagcca ttttctgaac tacctcttgc
    23821 tgtgtaagtc cattcatttt ctttttctcc attttccgcc aactttcaaa aataatcacg
    23881 tgtttaagtc tagcgtgttc caagattaaa aacaacttgt ttttataaaa tttatagaaa
    23941 aacacacgaa tgattttaca aaaaagatgc caaccttttt gtttgaatca cccgtgtgct
    24001 gtttttatta tcaatctatc tttacttata ttgttcctcc aaacggctca tccaccaacc
    24061 tagtgctgta ccggctataa aaagaacagg acaagctagt accccagcaa ctcccaatcc
    24121 cgcgtaatcg gtaggaatga tcataatcgt tgaagcaaaa actacaccta aaatgaaatg
    24181 gaatagcttg ctatatgcct ttgagaaaat gtaatccatg atttttgaca atccaagaac
    24241 acaaagaatc ccaccaattc cgatcggcac aatcacttca agtcgtactt ctttaaatcc
    24301 gtcagccatc gctttataca atcccatata caccaaaaaa ttagaagggc tcaatcctgg
    24361 gacgatcata cccaaaccaa tcaatccgcc ggcaatcagc catgttccaa aattctgatc
    24421 gacttgcccg tcaaataatt gagcgccgta taataaaaac aaataagcca gaacaaaagt
    24481 aacaaccatg atgatcacat cagaagtatc tcttcctttt ttccctgctt ctttccataa
    24541 cgcaggaaca gttccagcga tacatccgac aaaaaaccat aatatcgttg tttcatacgc
    24601 acctaataaa aaactgacta aaaaagaaag tagaaagatg cctgtcactc cgcctagacc
    24661 aacaggcaga aaaaacaaaa cattttcttt gaaatttttt gtaaggtggg ctaaaaaaga
    24721 aatcagccgc tcatacatgc caaagacagc tgctaatgca ccaccgctga ctcctggcaa
    24781 gataaatcct gaaccgataa acattccctt cacaaaccgt aacatccagt ctttggttgt
    24841 gtaccccact tcttgatttt ccattttttg ccttttttcc actatttgcg cactccttct
    24901 ttcatttaat tttattcatg attgaacatt tcctgctatt ttacttaaag actttttcat
    24961 aggtctctat tattttttca ttcattttga caatatcagg ataaaacatg ttacctccat
    25021 tatgatagag atagccgcca ttgtaaagca aggactggat ccgccaatat cggtattgtt
    25081 tcccttcctc atttcctaaa agtgggctca gcacatcttt agaatacgct tctgctaatt
    25141 ctactgtgtt ttctccgcca tgatttttta cataggcaat ataatctttc ccaaaattat
    25201 atgcttggac agctgtccat aaatcgcatc cttgtttctt tgcttctact atcatttccg
    25261 ccaaatattt gacaccttga tcaatactgt cagctggatt atcaatgatc tcagcttctc
    25321 catgcagact ttccgagctc tgcatgagat ctgtcccttt tccttttgat tcggtcaata
    25381 ttatcgcctt gatcaaattc tcgtagtttt ccatatgata attttttgca gctgttgtta
    25441 tttctatttg atagctttcc actcgtttta ccgaacgata agttcttcct cctaaaaaca
    25501 acaccgcacc aataaccgtc agaatgatca tgtaaaaaag aatacggata atagataatc
    25561 gttttcgctt catttcatca cctagttatt ttgacatctt tttttatctt actatttctt
    25621 tatcttcttg gctatttctt ccttgatttc tgtgtacgcc ttaagaaaaa atgaattttc
    25681 ctgtattttt tgacatctta cgaaaatgat cgttgttttt cagtaaaaac aattgcccaa
    25741 tgactgatat aatcgttata ctttacacag taggtgatta tgtgaaaaaa gaaaagaaat
    25801 atgcaaaaat gccagatggg agcgaaattt actatgaaaa gagcggtcaa ggcttccctc
    25861 tctttttact gcatggcaat gacggcagcg gccgcttttt ttcagaacag gtccctgtac
    25921 tcgaacgcta ctataccgtg tatctagtag atagccgagg acatggtcgt tcgacaaacg
    25981 aagcatctat gttgaatttt cagttaatgg ccgaggatct gaatacgatc atgttgctgg
    26041 aaaaaatcga tcaggcagat tttcttggat ttagtgatgg agcaaatcta gcactggtat
    26101 ttgccagcag cttccctaaa aaagttcatc gattgatttt aaattctgga aacacgcttg
    26161 ttaaaggcgt tcgtttttct gccagagtca tcagtaatat tcattatgca tgggtatggt
    26221 tactctcttt atttcgccca agtcttcgca aaaatctttt agttatcaaa cttttgctgc
    26281 atgacatcgg gcttacagaa aatgatttaa aaaaaatcaa ctcccccact ttgatcatcg
    26341 tgggaaaaaa agatgtaatc aagctaaaac attctttgta tatagctaaa actataccaa
    26401 aagcttcctt tgtgcttgtg aaagaacaag gacatgaatt agctagaaaa gatcctgaac
    26461 gcttcaatcg agaagttctt caatttcttt ctgaaacata actattagga atagaaggtg
    26521 atttatttgt taaaaaatac catacaatgg ctgaaaaacc acctaggact gttcaaaaca
    26581 atctttttga tttctgtcat tgtcattatt gtcggtgaat tgatgtccat cggtaaaact
    26641 ctatctatcc agcaattagg agaaacgttt gccactatac ctgtatggaa aagcggacta
    26701 atgctattga ttggtcttat ctctgtgtta cctatggttg gttatgatat tattttgaat
    26761 cggatgcttg aacaaaagca gaatcctcgt tacctgtttg agacaagctg gctgatcaat
    26821 acgatcaaca atattgccgg tttcggcggg tttgttagtg tggggcttcg ctctgaacta
    26881 tatggacaaa agaaagagag cagaaacgtg atccaagcat tatcgaaagt tttcttgttt
    26941 ttgatggctg gactttctat ttacagtctg ctttcttttt tacttgtagt cttcacgcca
    27001 gttgctcctt ttttgaagca atattggata tggttgatcg gcggcggtct atattttcct
    27061 gtcgtctttt tatttactac tttcaaaaag aaaggattcc ttggtggtac ctcacttaaa
    27121 actcaaggac agctattgct agtttcattg ttggaatgga ccggtgtcct gtttagtttc
    27181 ctttctgttg gttatttaat ggaaatccgg attgatctgt ggcaaacaat tccattattc
    27241 attgctgctt cagtgatagg aatcgtgtct atgattccag gagaaattgg gagtttcgat
    27301 gtaatgatga ttatcgggtt gtccgctatt ggtgtgccaa gagaaatagt cgttgtgtgg
    27361 atcttgctct atcgcctgtt ctactacatc attccgtttt tgattggaat cgtctttttc
    27421 ttcaaaaata tcggttccac ttttgatcag cgttattctg gtatccctaa acaattagca
    27481 acagaaattg cgcataaaat cgtggttgtc ttattatact tttctggaat catgctagta
    27541 ctttctgcca ctatcccgca agcttttaca gagtttcgct ggcttcatag tttgaaccca
    27601 ttgaaatttc attttattat ccaatttcct agtatactat tgggatttct tttaatcatt
    27661 atgggcagag ggatcgcagc acgtgtgaaa cgagcatatt tgcctacaat cttcttgatt
    27721 gttctagctt tactttatgt tttattaagt gattttagtt tcacaccggt ggttttcttg
    27781 tctattctgc ttctgatcat tcttgcttct aaaaacgaat tatttcggga acaattgatc
    27841 tattcatggg agtggcgtac gatcgatgga atcattattg gtgcactgtc cttgctttac
    27901 atcgttattg gggtatacaa tctgccagat ttccctcatc ggcgtcacca ttttatttcc
    27961 tttttcttat ttccttctga aaagatttgg ttttcgggat tgttggccat catcgctgtt
    28021 agtttcatga tcgttctatt tgttcatttc cttcaaggag aaaagaaaca gattggcgaa
    28081 gcctttaatg aagaaaaggc cctgaagatc cttacaactt atggaggaaa ctccgatagt
    28141 caattgatct tcttaaaaga taagcggatg tttgcctatg aaaaagacgg tgaaccaact
    28201 gttcttctcc aattcgcttg cttcaataat aaatgtattg taatgggtga tccttctggt
    28261 aagaaagaag attttccaga agcaatcgaa gcttttatcg aagaaacaga tcgactatgc
    28321 tatttaccag ttttctacga aacaagcgaa gagattgtta tgattttgca tgaattcggc
    28381 tacgatttca ttaagatggg ggaagaagct tatgtagacc tgaatagttt tacaacttca
    28441 gggaaaaaaa tgaagggcac tcgtgctgtc ttgaatcgga tcgaacgaga aggatttaca
    28501 tttgatgtct tacaaccacc attttctact gagcaaatga gtactttcaa aaatatctct
    28561 gataattggt tgggttcacg aaaggaaaaa gggttttctt taggcttttt ctctgaagat
    28621 tatctacaaa gagcgcctat tgcagttgtc aaaaataccc aagaagaaat tgtcgctttt
    28681 gcaacgatca tgcctactta cacgaacaat aaagtaggaa cgattgatct gatgcgatat
    28741 gattctaaaa aagcaccttc tggcagtatg gatttcttgt tcttgcattt gtttgcctat
    28801 atgaaagaag aacatatcca atggttcaat ttagg
//
LOCUS       DACEUZ010000022        27962 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.022, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000022 DACEUZ010000000
VERSION     DACEUZ010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27962
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.022"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(297..3575)
                     /locus_tag="IXG44_000840"
     CDS             complement(297..3575)
                     /locus_tag="IXG44_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HAQ1657576.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(3846..4391)
                     /locus_tag="IXG44_000841"
     CDS             complement(3846..4391)
                     /locus_tag="IXG44_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ1657577.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(4403..5095)
                     /locus_tag="IXG44_000842"
     CDS             complement(4403..5095)
                     /locus_tag="IXG44_000842"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HAQ1657578.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(5114..5398)
                     /locus_tag="IXG44_000843"
     CDS             complement(5114..5398)
                     /locus_tag="IXG44_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1657579.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(5411..5974)
                     /locus_tag="IXG44_000844"
     CDS             complement(5411..5974)
                     /locus_tag="IXG44_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAQ1657580.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            6202..6864
                     /locus_tag="IXG44_000845"
     CDS             6202..6864
                     /locus_tag="IXG44_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     protein"
                     /protein_id="HAQ1657581.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            6988..8184
                     /locus_tag="IXG44_000846"
     CDS             6988..8184
                     /locus_tag="IXG44_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HAQ1657582.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(8285..9661)
                     /locus_tag="IXG44_000847"
     CDS             complement(8285..9661)
                     /locus_tag="IXG44_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1657583.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(9698..10564)
                     /locus_tag="IXG44_000848"
     CDS             complement(9698..10564)
                     /locus_tag="IXG44_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HAQ1657584.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(10653..11678)
                     /locus_tag="IXG44_000849"
     CDS             complement(10653..11678)
                     /locus_tag="IXG44_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ1657585.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(11696..12370)
                     /locus_tag="IXG44_000850"
     CDS             complement(11696..12370)
                     /locus_tag="IXG44_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1657586.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(12554..13447)
                     /locus_tag="IXG44_000851"
     CDS             complement(12554..13447)
                     /locus_tag="IXG44_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ1657587.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(13521..15218)
                     /locus_tag="IXG44_000852"
     CDS             complement(13521..15218)
                     /locus_tag="IXG44_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAQ1657588.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(15211..16524)
                     /locus_tag="IXG44_000853"
     CDS             complement(15211..16524)
                     /locus_tag="IXG44_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ1657589.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(16524..20042)
                     /locus_tag="IXG44_000854"
     CDS             complement(16524..20042)
                     /locus_tag="IXG44_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAQ1657590.1"
                     /translation="MDYNGAYIKKWVEPVLPSSTASDLHPEDATTLYNVYLDVIGGEK
                     KEISPIDIVFVLDKSASMSELTAGTNSQTKNAALIEAVNEMSKDLLSDPSLDIRIGMV
                     NFYHNSTAINNHEQISSDIFPLTNDINRLTGSENTALNRTPIGGTPLTLGLKNGYETL
                     YKDNGGENRNPEKILIVVGDGTPTFSYAPIQSSYRTSTNGAWSNWTVMEDKIAEDNDV
                     LFRNFEEFSGNTSNAGFTHPVTYASDFNRPEDEVNVHYRYGEVKEGDNKATHWVGDGS
                     SNNNTNGSPTSQEKSSAINTVAYHHWLKNKYQENPPSIFSIGLGIDGNVSGRQRLDAI
                     GRNVLKNIADLEEDGVTPRYYNANNKNDIVTALEDISSTFKRTIQQATLYDETGFNVS
                     PFGAGRSADIQFYHLDPGENGMKYQEPEIWDEERHGQAPPEVVATPTTYVGRENQAYK
                     FSPISLGEGEMVRIKYQVKLDAGAQDGNFCTVNNMAYIQNLEDYENDITTGRMYFPSS
                     SIRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQNPESGEAEPIETSKTTTDGIAIFET
                     KMPILGDETGAVSDIFWIREIAGPPRYQLVDETYQFQIKKYAANEDEMLGHYELVDEY
                     TSGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNIEGTEYDELYVKLEMRNEFKPIHMNI
                     NKLIEGSNMPINGAEFELYETDGNGLEIGYPVAKGLSGVNEVEEEKGRLTFYSVDEDG
                     KYVIVDGEKVIHPIGEPDSFVYKDSEETTNYAEYKIIESQNPEGFKEPDENDTWILRL
                     YDNAIGTIQLKQTGEENWQYLTYTTDEEDRLWVAFTAYNTFDYREIRVRKLDHEGHTL
                     DGAGFDIRKVGDSSFPLYRAYTGNPLEPEESKRLPGIGYFYSRAVGVEKYVDLNYDQP
                     LHLSIGEYEVSESVAPNGYRLSDQVFQFALNEEGKFIIEDTVIEDETAPLPEGYEIID
                     GVLQVTLEDELAPIDLELLKIDSMNSRRLEGAEFSIEKQNSAGEYEIIEGGLAPDPND
                     SSLFNASDLLEGVYRIKEVKSPDSYRKLPGYFILEISYREEPDIENDRVVPGKEAGTL
                     KVEISYYPNEEAETPDLQQELEYELLETNRIRLQLSVGNDPENPLPATGGSGRLFYIL
                     LAAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(20335..20793)
                     /locus_tag="IXG44_000855"
     CDS             complement(20335..20793)
                     /locus_tag="IXG44_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ1657591.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
     gene            complement(21091..21609)
                     /locus_tag="IXG44_000856"
     CDS             complement(21091..21609)
                     /locus_tag="IXG44_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAQ1657592.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(21611..22228)
                     /locus_tag="IXG44_000857"
     CDS             complement(21611..22228)
                     /locus_tag="IXG44_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAQ1657593.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(22225..23079)
                     /locus_tag="IXG44_000858"
     CDS             complement(22225..23079)
                     /locus_tag="IXG44_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1657594.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIILLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            complement(23076..23987)
                     /locus_tag="IXG44_000859"
     CDS             complement(23076..23987)
                     /locus_tag="IXG44_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1657595.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(24028..25362)
                     /locus_tag="IXG44_000860"
     CDS             complement(24028..25362)
                     /locus_tag="IXG44_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1657596.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            25570..26400
                     /locus_tag="IXG44_000861"
     CDS             25570..26400
                     /locus_tag="IXG44_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HAQ1657597.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(26644..26893)
                     /locus_tag="IXG44_000862"
                     /pseudo
     CDS             complement(26644..26893)
                     /locus_tag="IXG44_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816675.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(27084..27278)
                     /locus_tag="IXG44_000863"
     CDS             complement(27084..27278)
                     /locus_tag="IXG44_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1657598.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
BASE COUNT         8134 a         6057 c         4848 g         8923 t
ORIGIN      
        1 agaggttgtg aaatcagcgt cctgacctga gtattagaga aaaaaacgga aaaatagttc
       61 ctcatatttt gccgtttgtt gagctaatac cgagggtcag ctgattgaac accgtttagt
      121 ggagagattg tgaaaaagct gtcctgcatc aagtaataag aacagccaat caaaagcagc
      181 aaggagaacc tcatctataa aaagatagaa ggaagtgaga catctattgg tctcatttcc
      241 ttctattttt actttcctca gtcaattgtt ttacatcata aaaggagaaa ttccacttat
      301 ttttttcgct gtttcatgac ataaacaact acaccgatta acaaaattcc tccagctaat
      361 gctagcaacg ggttattaaa ttctcccgtt tttgggagtc cttgagtgcc atttttttca
      421 ctttcttttg ctgtcctatc cggatgagaa ggttttccat ttggttttcc atttggtgat
      481 tcatttcctg gattttctct ttctggtttg tcatttcccg ggggcacatc ggcagtttct
      541 cgcacagtga tcgctttttg ctgggtaaca gattctgtat gagcagacca tttttcccca
      601 tcaaatattt ccgaacgata agtgacttct agcgtatatt ctccttttga aggtgctgaa
      661 aaaatagcgg tatgattaga aaattgagca cttgactgtg tttcaacgat ttggatcttt
      721 tctgggaaat aacgcatatc ttcaaagaca ggcgttcctt gatttggtgt gaaagttgac
      781 acaaaaactg taaaaggctt cagttcaatg atatttcctt ctgcattctc cgccacacca
      841 tttgcttctt gtataataat cggattattt tgttcatttg gttttgtctg atttggttct
      901 tgtacgattg catactctgg atattccgaa gacggtgagg tttctccgtc aaaaatagca
      961 cctacagata atggaagacc atccactctt tccttttctg tttgacgatt ccccggttta
     1021 atctgcatca gtccatgaac atggatactt ccattttcat agcgcgtaac aggctgctga
     1081 ccttctttcg aacatgtatt tagagactcg atccaatctt ttgatatggt atgtccaaga
     1141 gtattttctg attgtttctc tcgttcattc caatagtagt tcaacgagtg gtagatttga
     1201 tcttcgtttt gattcaatgg gcggatctgc tctctcatct caagattctc tgttcggaaa
     1261 gataagatac cagaagcatg gtagtctgtg tttcctgctg aagaggtata aagagccaca
     1321 ttatatgaac cgttattaaa acttgtactg ctattgacga taatatctgt tccgctattg
     1381 gaatcgattc ctttcgcaag attttcgaaa gacaaactat tgatcaattc atgagggccc
     1441 gttaaagaag aaccgcccat tttgaatcca ttaccgttac cttctccctc aattcctggt
     1501 acccagccgt ttcgataagc aactgagttt ttgatgatta cttttccgat cgaacctgtt
     1561 tcatttttag caaaaaggtc ccaaccgtca tctgcattat gatacgcgat acaaccgtca
     1621 aatacatttc cttctcctat tgtcagtttg gccgcaaatc catcggcatc ttcaaatcct
     1681 ggatcggcat tttcaaaaga tgtacagttc ttgactaagt tatgcgcggg ccatgcttcg
     1741 aaaggatcat ttgagctacc gctgatttgg attcctgtat ttccattccg ataagcatga
     1801 atctcttcga tcaaattatg atttccagaa atctgtaatc ctttttgcat agctgcacta
     1861 tttgtaatgt caaaccctcg taaaccccag tagtgactga acagtgtcat cccatttcca
     1921 gttcctttaa aatcaagtac aggacgcttg ttttctggat ttttctctgc aatcaactga
     1981 atcgggtttt ctcttgtccc gttaacgcct tttggaatcg tcaatcctct ttcgaaggaa
     2041 taagttccag gcgccatgac gattgtctgc ccagcatatg cgtatctcaa tgcttgtgtc
     2101 agagataatg gattttgtct gcttccgttt cctgcatctg acccttctgg tgttacataa
     2161 actgtcgaat atttaggttt acggtagtcc actgtatgaa gaatctcttt tgtttcataa
     2221 ctagtcatct ctacgtattc ttctggcgga tagtttttat ctggcgtaaa actgaaagta
     2281 aattcgttcg ttccttccat cagttttgtc tgataatcaa attcccgatt cgcagtgata
     2341 gccaagtttt ttgcgttctt aagcacctct cctgttgctt tatttttaat tgtcaataat
     2401 ccgtcagcat ttgctctaaa ggacatagta tgatcttggt ttcctgaatg attactagag
     2461 gtcacagtgt aaatcggatc aatcaagcga gtaggtcttt cttgcccagg aggatctgtc
     2521 gcaggatctg agtaagcaac ttcgattcct tctaccttga tcgtcatatt ccttgctgca
     2581 gcaaatcctg cataaataac cgattcatct gaagcgtata atttttccca gtcatacatg
     2641 atgtcagaag ccatttcttc tcctgtttca ggatcgtaat aatatgcctc taaaccagta
     2701 ttcgtttttt tcatctctac gtccagttta gtaatcgagt tcgtggccgg aaacagattc
     2761 ccatgaccgt ttccgaatat attataagaa cctgtttctt tttccaagaa ccctgctgaa
     2821 gtctctaaag gtgttgtttc cactgagaca ctcccaggag ctggcgcagc ttgcggatct
     2881 tcagatgata ttcctgtgat tgcccgtgtc cccaatccaa gtcgcatcgt atatttgctg
     2941 ccgctcgtgt tgaccacttc ttgtctatcc gagtcccaga catattcgat ccgactgcct
     3001 agtatggcaa atgaatttga ataaaatgcc tgcccaaaca aatgattttt tggtactgcg
     3061 tctcttacca ttaaagcaaa tccttcttgg gcattcgtat aggtccaaga ttctatttcg
     3121 atcgtcgccc gcatcctaaa gttgatgtct ttggggattg cttgataata ataggtcatt
     3181 ccatctgatc cgcttgactg gaattttccg ccgttattcg tggaagagag ccgcacgccg
     3241 tcttctaatg gttcaatggc atacctacta gcacttgtgc tttctccgaa aacaacccct
     3301 ttccactctt catttgcttc ttcagattgc ttttttgtct tttcattttc tgattctttc
     3361 atcataggat catttccttc ttccgtatcc atatcttcac tagttcttgg tatttcctcc
     3421 attgttgaca tcccttcaat gttcacgtgt gcattttctt cgttcacaga ctgttgttcg
     3481 ttagcagcaa cttcaacgac agaaattaaa accggggtac tgagcataag gagattcatc
     3541 attagcgcac tgtattttat acgttttttt aacaagtgta ttcctcccgt aacaaattag
     3601 aaagcgttta cttaaagtat tacctaattt atttttactg tcaataaaaa acaaaattat
     3661 aataagtgta ttttataaaa agaaaaaata taacttaagt tgttgataat aaagagattt
     3721 attaacgtaa aaagaataag tttttttaaa ataacgtata aaaaagagca taaaacaaag
     3781 tcgtttgttt tatgctcttc ttttattatt ttgtgctttt tattttatca aatcactttg
     3841 tttttttata aaatttctgc gcctagtgtg gactcaaaat gtctcaatgc ccattcatgt
     3901 ccaacgggat cgaaacttgc cacggcatct ttgaaaatat ggagtttgta tcctagatta
     3961 tacgcatcga ctgctgtatg caacacacag atatctgtac atacgccggt caaataaata
     4021 tctgtgatct ggcgttctcg caaacggatg tctagatctg ttccactaaa agcggaataa
     4081 tgccgtttgt ctatccaata aacattttcc tcttgctcat agtcttggta taaacttttt
     4141 aaagagccgt acaattcccg tccacccgtt cctatcacat tatgcggagg aaataagcgg
     4201 ttctccggat gaaaggaatc ttttggatca tgcgcatcaa tggcaaaaac gacaaaatcc
     4261 ttttgatcaa taaacttctt tgtatgagca gtcagctttt tttcaattac ttgtcctgct
     4321 gtcccagtag tcagctttcc atcctctgca acaaaatcat atgtgtagtc aatcgagatc
     4381 aatgcgttca tcttagtcct ccttatacga gatgtttgac aaattcgatc accatttctg
     4441 ctgatcgttt gcctgcgtct tcgataaatt cgtcaaagct ttgtgtcgcc gagtgatctg
     4501 ctgtgtcgct cattgcacgt acgatcaaaa atgggatatt gaactgacga gctgtttgtc
     4561 caacagcggc tccttccatt tcgcaagcta atgcttcagg gaaattcgtt aagatttctt
     4621 tgattttatc tggcgagtcg acaaatgtat cacctgtaac gatcaatcct tcttttgcgt
     4681 tcaaattcgt tgcttttgct gctttaacca tttcactgcg caaatatgtg cttgcttcat
     4741 aatatagagg cattcctggt aattgtcctg ggttgtagcc aaatcctgtt gcatctgcat
     4801 caaaataagc tactttgtct gatataacga tgtcacctac ttgtaagcct tcaccgattc
     4861 cgccagctga acctgtattg atcaccatgt tcacgccgta ttgttggatc aacagacttg
     4921 tggtaataga tgctaagact tttcctatac cggaacgtac gacgatcact tcgtggttac
     4981 ctaatgaacc tgaaataaat aaagcgcctg cgcgttccca tgataatggt tcgcttaggt
     5041 tctctctcaa aatttttact tcttcttcca ttgcgccgat aataccgatt ttcatctttt
     5101 taattccttc tttctatata aatgcgattg ctaaaaaaac cacacataac attaggataa
     5161 caataataag agatttcatc aaaaaagagt tccatttggt cgtcttttcc cgttcgctga
     5221 tccttgtttt cgtgattggt ttattctttt tgctttcttt acgataaaag ctggcaatct
     5281 ttttttcttc ttgctgataa gctgtttctg cttttcgctg tttttttaat gactcgcttg
     5341 cttgtgcttg ttgtcgcttg cgaagttcgc ttcgcgtaat aagcggtcct ttggccataa
     5401 gccgatccct ctactttcct ttggtcaata gttcccagta ttgaatcgca taaatcgtct
     5461 ttgcatcaca aatcaactga tctttcatcg cttgttgcgc ctcttcaaga gttagatgat
     5521 acaactccag tacttcgtct tcatcttgtg ctaaaggatt ctcgaccttc tctacccctt
     5581 gtgcatgata aatatgaagg acttcatttg caaaacctgg tgaaagatac atcgaagtaa
     5641 gatggctcag cgacttcgca cgatagccag tttcttcttc taattctcta gctgctgtca
     5701 tttcgggatt ttgtccttct cctggatcga tttttccagc tggaatctcc aaaatcactt
     5761 tttctaaagg ttttcgaaat tgtttgacaa gaagcagacg atcttgttcg tcaaaagcaa
     5821 tgatcccgac accgccagga tgaaatacca gttctctctt cgaagttcct ccatctggca
     5881 aacgaacttc gtctacagca acatcaataa tttttccgct atagatttct ttgcgactga
     5941 ttgttttttc ttcaaattgc tcattgttca gcatatcttc tcctgctttc tttgtaatca
     6001 taactgcttt tttttcattt ttcaattttt ctcaaaaact ccttattagt ttaccataga
     6061 taaaccttag attttctcta aatatagaaa atccttcttt agtccgatac tctttatagt
     6121 agtccgtatg ctaaaataag tatgtaatcg aataacgaaa agaattcaat actcaacgat
     6181 aaatgacatg gagggtttat tatgataaaa cgaaattggc cgtggcttct agcattggtc
     6241 ttgctatttc tctttgtccg aaacaatagt ttttcttttg ttctaggact tttattgata
     6301 ggtgtagttt tagttttgtg gggactcttt ggttcgcgca ggaaaaaggg aaaacaagaa
     6361 gaaattcctg cactgactga cgaacttgaa actcactatg aaaaaagcgg tatgacagcg
     6421 aatgaaattt cattttttcg acaaacaatg aatcaaacaa aaaatgagat caatcaattg
     6481 caaatgaata tgcagcagac agccaaactt aaagcaatcg atctccgcca tgatccagtc
     6541 aaagcagcga aagctttatt caaagaattg gtgaaagaac caaaccgact gcatgaagca
     6601 agtcagtttc tttatacgca tctgccgaat ctagttgatt tgacaaataa gtataatgag
     6661 atcaatgacc atgagatcaa aaacaaacag acctatgaaa aactagaaga aagcgcgcag
     6721 atcatcgacc agctgtcagc tttaatcgca caagattatc aaaaattcgt tgctgaggat
     6781 ctagatgata tcgacgtaga gatatctgtt gcgaaacaga gcttaaagcg tgataataaa
     6841 tatgaaccag aacaaaagga ttaactcttt tgttcataaa agaagactcg gaacattcag
     6901 atgatgtctg gctgtttcga gtactttttc taaagtagag acattcatgt aagaatcgag
     6961 aacagattca acaaggagga attttttatg gatcataaac cgactgaaac aaaagatgtt
     7021 acaccagtta acgatacact tgaagattta ttgaacaacc cgttttcaac tccaacagat
     7081 acgctgacca cttcacaaca agcggagatt gatcagctcc aagagcaaca gacagctgct
     7141 cgtttaattg acaaactacc ggcggaacgc caagaacaag ctaaacagct agcagcaaaa
     7201 atcgatgcta atgatgcaca gtctgtcatc agctacggat cggctgccca agcaaaatta
     7261 agtgaattct cacaatcgat gttgaatcat gtgcaagcac aagatattgg tcctgtagga
     7321 gattccttaa ctgaactgat gtatcgtctc caagaagcca atcctgacga actgcgtgcc
     7381 ggagaaggga acatcttcca gcgtgtcttc gggaaggtca aacaatcgat ctatgaagtg
     7441 acagcaaaat atcaaaagat tggtgcacaa attgacaaga tttcagtgaa attagataaa
     7501 gaaaaagacg gtctgctaaa agataatctg atgttagaac agctttacca gaaaaacaaa
     7561 gactactttg atgcactgaa tatttatatc gctgctggag aattgaaaat ggaagaactt
     7621 caaacaacga tcattccaga agcgatgaaa agagcagaag aaactggcga tcagatggat
     7681 gttcagattg ccaatgatta cacgcaattc ctagatcgtc tggataaacg tacgcatgat
     7741 cttcgcttag ctagacagat cacgatccag caagcaccac aaatccgttt gatccaaaac
     7801 acaaatcagg ccttggctga aaagatccaa gcatctatcg ctacagccat tccgctgtgg
     7861 aaaaaccaag tcgtgatcgc attgaccttg cttcgtcaaa aagatgcagt aactgctcaa
     7921 cgccaagtct ctgaaacaac aaacgactta ttgaagaaaa attcagaaat gctgaaaatc
     7981 tcagccatcg aaacagcgaa agaaaatgaa cgtggaatcg tggatatcga gacccttcaa
     8041 accactcaaa atgatttgat cgaaacaatc caagagacat tgcgtatcca aaaagaagga
     8101 aaagaaaaac gtcgtcatgc agaagtagag cttggacaca tggaagaaga tctgaaacaa
     8161 aaactgttgg atttaaccca atagatcacc tttcgcttgt tactaaaaca aacgcccaac
     8221 aacagtttat tacaactgtt gatgggcgtt tttcttatct ttcttctaac ttctccattg
     8281 gagtctattt ctcattcatc ttgctgtgtc catagccata gccaaaataa atgatcagtc
     8341 caataaccat tacgataccg aacacgatcc atgtcacagt ttgcagacga agcatcaata
     8401 caatacaagt caagatggag acgattggca atagcggaac gaatggcact ttgaattctc
     8461 cagcttttgg tttgccaaac atttttctca acttcagtaa gcctagacta acaagcatca
     8521 aataggcgag cgccatgata ttcgttagtt ccgctaatac attcaatggg aaaaatccgg
     8581 caaagaatac agctgccaca ccagcggcat aagtcgcttt tttcggtgta cgatgctttt
     8641 gatcgatttc tgtcataaac ttaggaagta agccatcctt gctgacagca aacagtaaac
     8701 gagcaagtga atacatcatc gagatcgtta ccgtcagtag cgttaaaatt gcccctacag
     8761 cgatcaccgc gcccactgtt ccatgtccta catacctcat tgcaaaagcc actgggtcat
     8821 tgactcctag ttcagtatac ggaacgattc ctgtcaagat cagcgtcacg atgatgtaaa
     8881 ggacagtagc aatcagtatc gaaccaatga ttcctcttgg cacatctttt tgcgggtttt
     8941 tgacttcctc ggctgccatt gcaacggcat caaatcccaa gaaagcaaaa aacacaagag
     9001 ctgcaccatc tagcacaccg gaaaaaccaa aaggcatgaa tggctgccag ttatcaggtt
     9061 taacgaaaaa tattcctaca atgataaaca acgcgatgat accaaatttg acatatacca
     9121 tggcattatt caaacgtaat gctttcttgg cttcaagcga cacgatgtaa gtgacgaata
     9181 tcactacgag caccgcaatc aagtcaatgt atgtaccatt ttccggattg tacccgccag
     9241 ttaatgcttg tggaagttcg gcccctaaac ttttcaaaaa accttgcata tagccagacc
     9301 atccggaagc aacagaggaa accgctagta aaaattcccc gatcaacaac caccctgtca
     9361 gccaggcagc aaattcacca aatacaacat acagataggc atatggccca ccaatcactg
     9421 gcactcgcga agcaaattct gcgaaactta gtccagacaa aatgacaacg attgcagcta
     9481 agacaaatga aagtgacagt gctggaccag cattttgatt ggccgcaacc cctgtcacca
     9541 caaatattcc cgtaccgatg atcgcaccaa ttccaagcat gatcaaatca gacgttttta
     9601 gttctttttt catctcactc gtatgattga gattgatctc tttcttacga aacaaatcca
     9661 ttgaagttcc tccccatttt aggttggaaa acagcgatta aactgttttc atatacccta
     9721 aaaggatacc acctaaaatg actaaaaggc aaccaacaat aacataaatg agctcttttt
     9781 tcgtttttct ttcgccaagg atgaaaatcc cacctaaagt agagataatg atccccattt
     9841 gtgaaagtga gaagccaaca gctaaaccaa gtgatttgac tgttagaagc atgcagatgt
     9901 ttcctaagcc ccataataat ccacagatca tgtttttcca cacaaactga tcgactttaa
     9961 ctttacggaa agcaaagaag cttgcgccta agatcattcc gatactttga ggtaagataa
    10021 ttgacatcgc atccaaattc gcccagttga cgatgatggt atatacacca taaccaatcg
    10081 tagagaagat caatgctcta aaacccttac caaaatcaag cattttatta tctgtatcta
    10141 tttttccttc gtcatcttgt cgggcagtca aatacgcacc gcttacaaga agagctaaag
    10201 caataatacc gtatacataa tctttcgttt gtgtccattc atggaagaaa acggctcccg
    10261 caatcgtatt caaaattaat tgaaaaccag tagacaatgg tagccctaca gaaacaccca
    10321 tgtacttcat tccatggaac tgtccatttt gtccaacact ccaagctaag ccggataaaa
    10381 atccgatcaa gaaaatccaa ggggtgatta caggactaac aacgaaaaag acaactaaag
    10441 aaaacagaaa tgcaccgatc gtcattccta gtgtttgttg attggcatcg ccaccaattt
    10501 taccacttac taaaccaata ctaccccatg ccaacatagg gactaatgct attaaaattt
    10561 ccataaccaa tctcctttta tttcacatga taagaaactt atcagcgaaa acgttaacaa
    10621 cacaagcact ttttttcgtt tctaacgatt ttctaacgtt tttcattata aaaatttaag
    10681 attgggaaaa ataaagtcat tgttgtccct tttccttcag ttgattcagc cgtcaaatgg
    10741 atacctaatt tttcggccag attttttgct aaataaagtc ctaatcctgt tgaatgtgtt
    10801 ttgctaagtc gaccatttct cccagtaaat cccttatcaa agatccgttt caagtcttcc
    10861 gctgggatac cgatccctga atctttaact gaaaggctga ctccttgcgc ttccttttct
    10921 atgctcacaa tgatgtttcc gtgatccggc gtatatttga tggcattact aagcaattgg
    10981 ttgaagataa aagcgatcca tttagcatcg gtcaaaacag tatgatcctc accaacgatc
    11041 tcgtagcgaa tatttttttg gataaaataa tttgcctgac tgcgtatcac aggctgaatg
    11101 attcctttta gtccatattc ttggatgagg tagtcccgcg agaaactgtc gagtcttgca
    11161 taatacaaaa cttgttctac gtattcatcg atttttgata attcattttc tagtaatgta
    11221 tacttttgat cgtcaatatc gaactcgatc gaacgaacta gcaatttaga agcggaaagt
    11281 ggcactttga tttcatggac ccatgagtca atataatctt tttggtcttg ttgattactg
    11341 attgctgatt gcattacttc ttggtgttca cggatcagct gattgatata ttcttctacc
    11401 agtttttctt cttctgtcct cccaccactc aaataatttt gcaaagggga agttttttgt
    11461 atgtcggcta attttcccca ccatcttctc ttgctcaagt aaccacccag cagataaaaa
    11521 atcagaaaga cgccctcaat caaagctaaa taagcaattg tgctcaaatc aactttgatg
    11581 tttggcgcca gccacatcac aaagatcgtc aatgcaataa aaaacagcca tcccaagatc
    11641 aatgtccact gatctttcaa atatttaaaa aaattcatct taaccgctca tcctttcagg
    11701 gaacgatata tccctgtcct acttttgttt cgatatatcc ttggatacct gcctgttcga
    11761 tttttcggcg taaccgattg atgttgactg tcaatgtatt gtcatctaca aatcgttcgt
    11821 catcccacag tgctcgcagc aatttttccc gacttaagat ctttccattg tgcttcatca
    11881 aaataaataa tagacgatat tcatttttgc tcaaatcaat gatctctccg ttgatttcca
    11941 tacttccgtt gtcaacattc aacgtaatgc cattgtggct catcatttca ctagataatt
    12001 ctgtgtagtt gtaagaacgg cgaagtaatg cattgatttt tgcaatcagt acatcgattt
    12061 ggaaatgttt tgtcacaaaa tcatctgccc ccatgttcat agccatgatc atatccatat
    12121 tcgtattcct gctggagata aaaatgatcg gtactttcga tacttcacgt attttttgac
    12181 accaataata gccatccaaa acgggaagat taatatctag taatacaagc tgtggttctt
    12241 ctctttcaaa gtctgagaac acttgattga aatcggtcgt tccaaaggtt tcaaattgcc
    12301 atttctcaag ttcctccatg atcaattgtc gaatgatctc ttcgtcttcg actaccatga
    12361 tttttgccat aaaaaatccc tccatctttc ttcttgctac tacaatatca aagaagccct
    12421 tgtgaaacaa gttggcttac ctggaaaatg tcattttagc aaacgttaaa aaaaactgga
    12481 aatatgcgat ttaatcgcgt ttgtctcttc caagcagctt aaatttatta tgttattctt
    12541 aataattggt ctattatctg ttatttttct ttcgttgttt ttgtctttta aattttcgat
    12601 aacaccaata accgaaaagc gttagcacta gcaatatacc tagaaggagt ataagtaatc
    12661 gataaagatg gtattttttt gtctgttcta tctttgaaga gacttcttct gtaaagggaa
    12721 ctcgtcttcc tgtcactagt aatcggtgag tgttcacggt gtaaggtgta caagtgataa
    12781 gtgtgactag atcctgcttt tctctaattg ctaatgtatt tgtttttgtt ggaaggacaa
    12841 caattttctc aacgacttca taagcgagca tccgaccata cacttcaaga tagaatcggt
    12901 catcttgctc catttcttca agatccgtaa agagtttttt ctcagcaata ccgctatggg
    12961 cggtaatcac ggaatgtgta ctctctcctc cagttggaaa agaggtgcct tgcaggactg
    13021 tcgctcctct atctaacaaa agatcattcg tttcatcaaa taaaggcaag ctaacattga
    13081 ttttcggaat atagatggct ccgatcatat gttgttcgta atattctttt cctggatttt
    13141 ggacatttct aaccgcttgc tcaaaaggat ctttgacttg tcccattcct gggattactg
    13201 atgtatgagc aagcgtctga tttttctttt catgcactgc taattgttgc tcatgttttt
    13261 cttctttatc gcgttcaagc tgcttttgat aattctcgat ccttctttga tccaaataat
    13321 tgctgagagc atctgctaaa aatggatatg taaaaactgc agctccggca agaaacatca
    13381 ctgccataaa taattgtaat aaaagactga tttgggtttg aggtctatta cctattttct
    13441 ttttcatttt cttcaccttc ttatttaaaa aaactgcagc aacctttata aaaccgattg
    13501 ctgcagctta tttttgttgc ttatacttca gacttcattt tagatttacg gtaccagctg
    13561 tacgctccaa tcatcaaact tattccgatt agtaagaatg ccaagagccc gttcccacca
    13621 gtagcaggaa gtgacccgcg tttcatattc ggtactcttt ctggccctgg attttctact
    13681 cctacgatct gaatttcttc attatagctt ccataagaga tgataaattc agtaaaggct
    13741 gcctcacctg ttggaagtac ataaccttct ggcgccatgg tctctttcat ttgataggtt
    13801 ccataatcta acccttgaac ctcaaagtat ccatttccat ctgcgacaaa ttctgttgct
    13861 tgtccgcttg ttgcccactc aattctatct gctcctgatt cataaacagc taaaggcgac
    13921 tcccctttat aaaatattgc gtattcagaa cggtcttcat tccacaattc gaatcttgca
    13981 tctttaagca attcactgct tgatgcatca tatttttcaa actttcttcc acctgttgtg
    14041 actttctctg gcggctctac tccttcttca taatcaaatt catcatttcg tccgatttca
    14101 ataaccgctg tgttgttatg gaaatcatcc gcttctagtt ctgtggaaac cttcaaatta
    14161 tacgtgatcg ttaaatctcc tccacgtgca tctgctaatc tacctaaagt atctactttg
    14221 gctactccat tttcttctgt caaaatcgtc acgacaaacc cgttgcttac tacttctact
    14281 atataatctt gtccttttgt taaaatcgtc tcgccggctc gcacttctat ttcctgatca
    14341 taataagtta gaccaactgt aggcgtatct gtgattttgt aatttttgat aacagcgctt
    14401 ccgttttcaa ggacagaatc gatgaaataa ggaataggtg ctgtgatcgt gtaagaagcc
    14461 gtttttcctc tttctagatc aatataagaa atcgtttctt gttctccagc ttcattaata
    14521 atcgttactt gtctaaggtc gctttcctct acattcatct gtttatctgc ttctctgatt
    14581 tcgtttttcg gatacaaatg cacatctgta ttgtatgtgt ctttccatgt agaaccatcc
    14641 cacacgccat ccaccatatc aggcatcatc actggcatca taacaaccat tggagacgcg
    14701 attctggaaa tatgagctgg cgaataagtt tctaagaaaa gatagacttt atctctagaa
    14761 gaaggtgctt cttccgtcac ttggacggta gagaataccg cttctccagc ttcattcgtt
    14821 gtttgtttgt caatacttgt cagtcgatat tttaaaaacc aactttctgt gtgccgtttt
    14881 tgaatccatt caatcgcttc ggcagaatta agaccgtctt ctcgttccgg tgttaaagga
    14941 ttatctcgaa tcaaatcgta atatacttcc gttacgtcaa acaaatcaaa atcgactcct
    15001 ggaatatttt cttcaccaaa atccgtagaa accaatccgc tattcggtcg ttcttccgga
    15061 acagatgaga aacctttttt gtgtaatgta atcgtcactt cacttggttt ttgtgcgact
    15121 tcttcggctt tgactagttt tccgttcccg aaaatcccta ttcccgcagt aacgagtaac
    15181 agtacactca ttcccgtgac aatcattttt ttattcattt ttttctcccc gttctttttt
    15241 tattaataaa acagcaccta acaataaaag aattcctagt aaagaaatca acgttcttgc
    15301 ttcattcgtt tgcgggaata cggaccattt tcctgattga cctggattgc ttggaccact
    15361 cggctctttc ggtttgtccg ttgtttcttt tacggtctcg ttataaacac gtggtgttgc
    15421 ggattcatag gcagagactg gaactttccc ggattctaat tcagccatct cctgtccgtt
    15481 aactactaca tattgtggtt ggccttttgc atccacccaa aattcaggga tgatgacctc
    15541 gattctctta cttgcttcat ccatctcgta accttccaca ccttttaatt cttcaaaata
    15601 ataggtgcca gaaggcaaga aacgttgtcc tgtcgtaaca aggccctctt tatccgaaat
    15661 gaatttgctt acatttttgt cattcaaagg atcattgtct gctggtttga tccattgatt
    15721 tttcaagtca ttagcaggag ccatatctaa ataatatttc cccccttcta tcatctgata
    15781 tagagcaaaa acagcacctt ctaatggttt tcctttttca gttgttcctc tttgacgtcc
    15841 atacttgaag aagtatggat ctcttagata accgacattt ttaggataga tatgaatctc
    15901 ttgcagttcc tcggttgatt ctgtcgggtg ataaattggc aaaatagcag caattgggat
    15961 agctgtctga tcgatatcaa catccaatcc aacttctgga tcaatcgatt cttcgaaaat
    16021 caaataaact gcatcccgat cttggttttt cttaggtgct ttgatacgtg caatcccgtc
    16081 tcctaatccg ccaaccgtat ctttcccagt tgttaccgaa gtccctattt ggacaagcct
    16141 ttcttctgtg attagctgac gaatattgtt tctgttcgtt tcagcgattt gctggacgaa
    16201 ttctttagga gtcatgtttt cagcagcttt tttttcatga taataatcgg tcatgtcgta
    16261 aatggcaaat gtcgctccat ttagtggcac cgtctgtgaa atgatatctg ctgtttcccc
    16321 tgcttcctca gagatagcca agccatcatt ttcatggtat tcaaattgat cgttataatc
    16381 gatatctcga atcatccgtt tatgcaacac gaattccacg acatcttccc ctgattcttc
    16441 tgccgacatc gcatgtggaa gaaggactgc tgcaagcggt agtaaaacaa gtaattgaag
    16501 catcaaacac aacagctttt tcattacctt gctccctcct ttttagattg tctgacaaac
    16561 aaaccatagg ctgctcctgc aagcgccatg aagaatgcag caagcaggat atagaataat
    16621 cgtccggatc ctcctgtagc aggcaacgga ttttcaggat cgttcccgac actaagctgt
    16681 aatctgattc gatttgtctc caaaagttca tattctaact cttgctgtag atcaggcgtc
    16741 tctgcttctt cgtttggata atacgaaatc tcaactttta gtgtacccgc ttcttttcct
    16801 ggaactaccc gatcattttc tatgtcaggt tcttctcgat aactaatttc taaaataaag
    16861 taacctggaa gttttctata gctgtcaggt gacttcactt ccttgatccg atagacccct
    16921 tccaacaagt ctgaagcatt gaatagggaa gaatcgtttg gatctggtgc caagcctccc
    16981 tcgatgattt catactctcc tgcactattt tgtttctcaa ttgaaaattc tgcgccttca
    17041 agacgtcggc tattcatact gtcaattttc aaaagttcca aatcgattgg cgctagttca
    17101 tcttctaagg ttacttgtag tacaccatcg atgatttcat agccttccgg caagggagca
    17161 gtttcgtctt cgatgaccgt atcttcaatg ataaacttac cttcttcatt cagtgcaaac
    17221 tggaacactt gatcagatag ccgataccca tttggtgcga cagattccga gacctcatat
    17281 tcaccaatag acagatgcag aggttggtcg taatttaaat cgacatactt ttctactcct
    17341 acggcacgag aatagaagta tccgatccca ggcaaacgtt tcgattcttc tggttctaac
    17401 ggattaccag tgtatgctcg atacagcggg aaggaagaat cccccacttt cctgatatcg
    17461 aaacctgcac catctaatgt atggccttca tggtctaatt ttctaactcg gatctcccga
    17521 taatcaaacg tattgtaagc agtgaatgct acccataaac gatcctcttc atctgttgta
    17581 tacgtgagat attgccaatt ttcttcacct gtttgcttga gttggatagt ccctatagca
    17641 ttatcgtaaa gtcgcaagat ccatgtgtca ttttcatcag gttctttgaa accttctggg
    17701 ttctggctct caatgatttt gtattctgca tagttggtag tttcttcact atccttataa
    17761 acaaatgaat ctggctctcc gattggatga ataactttct caccatccac gattacatat
    17821 ttcccgtctt catctactga atagaacgtg agtctccctt tctcttcctc tacttcgttc
    17881 acaccagaaa gccctttcgc tactggatat ccgatttcca gtccgttacc atctgtttca
    17941 taaagttcaa actctgcgcc attgattggc atattactgc cttcaattag tttattgata
    18001 ttcatgtgga ttggcttgaa ttcatttcgc atttccagtt tgacatacaa ctcatcatac
    18061 tctgtgcctt ctatgttagg ctcttgtaca acagaaagct tgtgatattc tccttcataa
    18121 taggaattcg gatctttacc ttccggtccg ccgactctcg aaccagatgt atattcatct
    18181 acaagctcgt agtgacctaa catctcatct tcgttggctg catatttttt gatttggaat
    18241 tgatacgtct catctactaa ctgataacgt ggtggtccag cgatctctct gatccaaaat
    18301 atgtcgctga ctgctccagt ttcatctcct aaaatcggca tcttcgtttc aaagatcgct
    18361 attccgtccg tggtagtttt acttgtttcg attggttcgg cttccccact ctctggattt
    18421 tggtcatata atgcaaattc gactccagcc aacggctcgc cattttgacc cttcttgctg
    18481 acttggaaat tacggccttt atgttcgtaa cgaatggaag aagatgggaa atacatccgt
    18541 ccagtagtga tatcattctc ataatcctcc aggttctgaa tataggccat attgttcact
    18601 gtgcaaaaat ttccgtcttg agctcccgca tcaagtttta cttggtactt gatcctgacc
    18661 atttcccctt cacctaaaga aatcggagaa aatttataag cttggttttc ccgtccgaca
    18721 tacgttgtcg gagtcgccac tacttctggt ggggcttgtc catgacgttc ttcatcccat
    18781 atttctggct cttggtactt catgccgttt tctcctggat ctagatggta aaactgaata
    18841 tctgccgatc tgccagctcc aaatggtgag acattaaaac cagtttcatc ataaagtgtg
    18901 gcttgctgga tcgttctctt gaaggtagaa gagatatcct ccaaggctgt aacaatgtca
    18961 ttcttgttgt tcgcattgta atatcgcggt gttacgccat cttcttctaa atcagcaata
    19021 tttttcaata catttcgtcc aatagcatct agtcgttgtc tcccgctaac gtttccatcg
    19081 attcctaaac caatggagaa gatacttggc gggttctctt gatacttgtt tttcagccaa
    19141 tgatgataag caactgtgtt gatcgcagat gacttttctt gcgaagtagg actgccattc
    19201 gtattgttat tagaagaacc atcaccaacc cagtgagtcg ctttattatc tccttctttt
    19261 acctctccat aacgatagtg tacatttact tcatcttctg gacggttaaa gtcagatgca
    19321 taggtaactg gatgagtaaa ccctgcatta gaagtattcc cagaaaactc ctcaaaattt
    19381 ctgaacaaaa cgtcattatc ttcagcaatc ttatcttcca tcacagtcca gttgctccaa
    19441 gctccattag tgctcgtacg ataacttgac tgaatcggtg cataactgaa ggtcggtgtc
    19501 ccatctccca caacgattag gattttttct ggatttctat tttctccgcc attgtcttta
    19561 tacaatgttt catagccatt tttaagccct agtgtaagcg gagttcctcc gattggtgta
    19621 cgatttaatg ctgtattctc acttcccgtc aaacgattga tatcatttgt caatggaaag
    19681 atgtccgaac tgatttgctc atggttattg atagctgtac tattatggta aaaattcacc
    19741 ataccgattc gaatatccag actcgggtca gagagcagat ctttactcat ttcgtttact
    19801 gcctcaatca acgcagcatt tttagtttga ctattggtcc cagcggttaa ttcactcata
    19861 ctcgctgact tatctagaac aaaaacgata tcgattggac tgatttcctt tttctcgccc
    19921 ccgatgacat ctagatagac attgtaaagg gttgttgcat cttcaggatg tagatcagat
    19981 gctgtgctgc ttggcagaac aggttctacc cattttttga tgtacgcccc gttataatcc
    20041 aagtaactgt tttcaaaatt ccgttcattt ccccaaagct gaacggtttg agctggaacg
    20101 tttctatctt cttcgcctgc agttgatcca ttattagtag catagtcgta atttcgccag
    20161 ttcgctcccc cgtttgtata atcactatca tcgatcaagt tctctccgtc atatggcgag
    20221 cctttgtctc cttgatagcg ttcattgctt tgtgtccaat ttcctaacac ttttgtatag
    20281 tcattcgttg taaacttcat ctattctttc atcaaccacc acttgaagag gtagttaccc
    20341 ttcgttcaca tcggggatct tttctgtttc aaaaatcaat gtgccggttt ctgcttcttt
    20401 tgaataaact tttccgacat accattcttg accatcgatc gtctcgactt ttaggtcttc
    20461 tccatcttca tcataggagg atagacctgt tccacgaacg ttttttacgg taccaatccc
    20521 ggcggctgcc gcatcgattg ctaaacgtat tctaccttca ttggcacttt cctttttttc
    20581 aaaagccaga tgccattcaa gggtttcttc tttttcaata tacttccctt caatttttgc
    20641 atagtcctca tctaagaata cctcatcctc ctttgtttct gttgactcag cagctacatc
    20701 cagggtcgtc atatgataga ctgactggaa atatggtcca aatagcaaac tgacagttac
    20761 taactgcaaa agcttcaatt ttaacccttt cacatcctta tactcctttc aaaaatattt
    20821 gtcttctttc acaacaatca acaaaacaat gcccatctaa ttataattta atttaatttt
    20881 aattttaaat caaatgatat cctttttagt taaaaaacac caattataca acatttatta
    20941 ttaaaattaa attataaaat tttataacgt tctcatttaa caaaacttaa gttattaaga
    21001 caaatgaata aacaactacg ctttcctcga aacaaaaaaa taaagcacaa atgcgcattt
    21061 acgcactcat gctttattcg tacaattaat tcagataacg gcagcgcata ggatgccaat
    21121 aacacttcct aaaatagctc ctcctaaaat gtcagtagga taatgtaaaa gtaaaaaaat
    21181 cctcgaataa gcgatcaaac aagcaaataa aaaggctaat attcctgcaa ctggacttaa
    21241 ttcgaacaac gcagctactg ataaaaaggc tagcgctgta tgtcctgaag gaaaagaaga
    21301 agtcttcggt ttacttgtgt aaaggacaag ccgacgattt ttttcataag gtcttttccg
    21361 tcttgtcgtt tttttgataa aaagcaaacc aacaaaaaaa tagatcaaac tgccgataag
    21421 aatggctaga ccatattctc tagtatcagg tatcaagact aaaacagcag caaggagcaa
    21481 ccaaataaaa ccaccattag agatattact gataaatttc aaaaaattga tttttcgact
    21541 atccgttttc cgttgcttgg cgaacttgtt gataatacta tcatcgattt tagcaatcaa
    21601 acgcaacatg ttattcctcc tttttaagaa ccttttcttg cgtacgatgg ataaaaagca
    21661 ggacttgaaa ataaaacaag aaactgaaac ctaataaagc aaaatagatt ggtcctgcaa
    21721 accagaatgc gacaagaagg atcaacgtgc ctgccaaata gaaaagcgcg cttttgaaat
    21781 cattaaaaaa caagaaaaat gcagtaatcc aagaagcttc tttttgttcc agttgaagca
    21841 agattttgta actaaagaga agcgcgctta atagaagcaa aaacaacgta gtatacatca
    21901 atgtatagcc aattccccca ccccaatagc ggaaaatctg atagtcgatc aatagaagaa
    21961 gaaaataaac acttgctaaa ctatcttgct ttccataaag agagaagttc tcacgaaact
    22021 ttttccagaa tagtgaaccg agctttttct cttcattttt tttgtaatga taaatcatat
    22081 acagaactgt tcgacttgca gttcctaatg tgaaaacagg caagacgcac aggaaccata
    22141 gtagctgtaa atggataaga cgtggaatcc attcaatgat ttctaagaaa taatcattgg
    22201 cttttttacc aatcgatcga ttcatcatcc ttttgaacct cctgtcaaat ccatcccttc
    22261 aatgaaataa cgttgtgcaa aaaagaacag caaaatcgtc gggatcaaga caagtgttgc
    22321 tcctgccatc agataattcc attgtgtcgt tgcttggttc tgaaaaactt gcaagccaat
    22381 ctgtaacgtg tatttttctt gatcttggat atataacaga ggacctaaga aatcattcca
    22441 agctgcgttg aaggaattga tcgcaatggt aatcaaggca ggtttcgtca atggaagcat
    22501 caaatgactc caaatataca aatgattcgc tccgtcgatt tcagcagctt cgatcaattc
    22561 attattgatc gataagtaaa attgacgcat cagaaatata ttgaacgcat tgccgaagaa
    22621 cgatggaacg atcaggggca aataagtacc tacccagccg atcttggaga ataatacata
    22681 ttgagggatc atcgttacaa accccgggat catcatcgta gaaagaacca aagcaaacat
    22741 cagctttttc ccaggaaaat caagcttcgc aaatccataa gcaataaaag aatttgaaag
    22801 gacattaccg atcactacta gcacagtgat gaataatgta tttcttgcat agcgccaaaa
    22861 aggaaaggcg gcaatcgtat ccagatagtt ttcccatacg attttttctg ggaaaaaggt
    22921 cggaggataa cgaacgattt cttccattgg tttcaatgaa gtaaacacca tccaaagcaa
    22981 tagaatgatc aaaatgattc ctattactgt catgaggaca taacttagta ttttttttgt
    23041 ttgccgattc acactggtct ttttgaaata atccatcatt tgccgtcacc tccagaataa
    23101 tagacccact taggtgcaag cttcaaattg acaaacgtca gaaccaacac gatcagaaac
    23161 aggatcattg acatagccat cgcataaccc atatcaaaag ctacgaacgc cttgttccac
    23221 atatgcagat tataaaaaag caaagaattt gcaggtccgc ctgttccatt tttcgtcatg
    23281 acataagctt cttgaaaaat ttggaaagaa ccgatcgtag aggtgatcac atcgtaaaaa
    23341 ataataggag tgattaatgg caaagtaata cttttgaact ttccccaaac gcctgctcca
    23401 tcgatttctg ctgattcata taaagatcta ggaacatttt gtaatgtagc taagtaaagt
    23461 agcattgctc ctccagaact ccatattttc atcaaaatca acgcaggttt cgtccattgt
    23521 ggatcaaaca gccaagctgg tccagggata tggaaccagc ccagaaatgt gttgatcaat
    23581 cctgcagatg gagaaagcag ctgcatccat aagatgtaga ctgctactcc tgataagact
    23641 gccggtaaat agaaagctgt acggaataca ctgattcctt tgattttttg attgagtact
    23701 gtggctaaaa acacactgaa aatcgtcgtc agcggtacat tgaagatgac gaaataaacg
    23761 gtgttgtata acgatttcca aaataaatca tcgtgaacga acattctttt gaaatttgct
    23821 agaccaatga aattcatctt ggatgtcaaa ttataatcgg tgaaactacc aataactgaa
    23881 aaaagcatag gccctagcat aaataccaag aacccgataa taaaaggcga gataaataaa
    23941 taggcccatc ttgcttcttt tttctttcgg atagataaac gcttcaaatt ttcttgcctc
    24001 ctctgtacct agacagtcaa aaatggatta ttgattttgc ttgactaagt tttctactgc
    24061 tttttgtgca tctgccaatc cttcttttgg tgattttgtt cctaaaagaa tctggtcaat
    24121 ctgttcattc actaatgtcg tgtaatccgg agcatctaaa ggaactggtg tgatcaccgt
    24181 atttttaaag ttttcgtccg ccatttgata gatcattttt cctttatccg ttaggttgtc
    24241 attattcact aaattctcat tggctgtctt gctcgccatg atatcaaaac ttttttcgcc
    24301 gaatttttct tgtacttctg gagaagaaag atatttgatg aattcataag aggcctcagg
    24361 atcttttgct ccgtatggta cttccaagac gaatccgcca ccccacgacc aatggcctga
    24421 accttcttct ttttctggga gctgcatcac gccaaaatca aaatcatccg gtgcattttc
    24481 catcaaacta gagtaataat tgatattttg ggcacgcatg gctactaatc cggaaataaa
    24541 tggatcagat acaccagacc caaattcggc ttctaattta ttgatcgtat ttttcccata
    24601 ataatcttgc cagtcttgga tccactccaa ggcttccact ttatttttag tatcaatctt
    24661 gactttttca tctttgtcaa accaactagt cccgttatct gcgtttagcg cccatacatc
    24721 agcccctaaa ttccataatg gataaaaacc gatgcgtgta aattcttttc cttctttttt
    24781 atctagttta cgtgctgttt cttctaattc atcccaagtt gtcggtagat cttgttcact
    24841 gattccagct tctttaaata actttttatt ataaaaaatg acttgtgtat ctgtattgaa
    24901 tgggatcgca taaggatcac cttcatattc tactgtatcc caaagctgcg gataaaaatc
    24961 cttagaagtg tctttttcta catatttgga gagattagtt gcttgctgtg cttctgctct
    25021 ttgggcaacg gaatttatgt cctgtacaat aacatcgggt gcatttccag ctgttattgc
    25081 agataaagat ttagtccaga tgtcacccca tggctgatac ttataattta catggatctc
    25141 gttttgtgat tgattgaaat cctcaatgat ttcttcgata gcttcttttc gtgcaccgga
    25201 tccccaaaag gaccagaaat caatattttt tcttccatca cttgtttttt cagaagaatt
    25261 tgctgcactt gaactggcat cgcttgcatt acttgatccg catcctccta ataaaaacaa
    25321 tgctgaactt gccagcaacc ctgtaaacaa taattttttc atattttacc tccaaatttt
    25381 aatgtaaccg atgccggttt taatataacg atatcatttt gttctgaaaa taacaattta
    25441 taaggtatga ttctaaaaaa attattaatg ataccaatac tttatataat tcgcgagcgc
    25501 tttctttgaa ttttttaatc tttcgtagca gaatgagaat gtattaaaaa tagattagga
    25561 ggaattttga tgtttagaca ccagaaagaa ttgcagtttg aagcaaaacc agatcgtccg
    25621 aatccattaa tcgcgaaata tctgcaggag ctgatcggcg gccaatatgg cgagatgagt
    25681 gtagctatgc agtacctgtt ccaaggctgg agctgccgag gagaagaaaa atataaagat
    25741 atgctgatgg acattgcaac agaagaaatc ggccacgtgg aaatgttggc gacaatggtt
    25801 gctcgtttat tagagggtgc gcctgttgaa gatcaagaag cagcgatgca aaaagatcca
    25861 acaatcggtg ccattatctc tggaatgaat ccacagcacg cgatcgtagc tggcttagga
    25921 cctcgtcctg ctgacagcgt gggcaatcca tggagcggtg cctatatcat tgcgagtggc
    25981 aacttgttag cagactttag agctaattta aatgccgaat cacaaggaag acttcaagta
    26041 gcacgtattt acgaaatgat cgatgatcgt ggcgtaaaag atatgctgtc tgttctcctt
    26101 gcaagagata gctatcatca aaatctatgg gaatgtgcag tgaaagaatt agaagaaaaa
    26161 gaaggttcaa tcttagtccc aagtactttc ccgcgtgaca aagaacgaaa agacattgct
    26221 tatgacttct ataacttctc agagggagac caaagtagtg aaggttcttg ggctagtggc
    26281 aaagcactgg atggcgaagg cgaatatcaa tggttgaaag aacctaagat cgaaggcgct
    26341 gcaccaaaat tagaaccagt aacaccaaaa atgtatggga cacctccatt caagaaataa
    26401 atatctaccc gtagaaacac ccgttttttg ttccacacgc tttgtgagca aaactgacta
    26461 aagtcattaa tcataaccac ccttcaaaag ggtggttttc tctagagcta taagctcaaa
    26521 tcaccggcca gcgcctaaag acgctggctt tcatatttta tgttcaagcc atattgcctt
    26581 tgccagtttt gcccacctct taaaaggttt atgtactact ccttagccct taaaaggatc
    26641 gtcttactct cgtacactca gcttatctaa cgcactatca tgtttttctt gttcttgaat
    26701 gtactttttg atggtcgctt cattttgtcc tacttttgtc acatagtatc cttccaccca
    26761 aaagtgtcga tacccaaatt tatattttag atttgcacaa tctcttgatg atctcctgaa
    26821 tattggctcg atattgatta taaattattt ttcttctata ctttggtgtg aatacaatgt
    26881 ggtatttaca catctacttt gtacgggcta aactatttcc ctttattgct ccacccgcat
    26941 agcaagtggt tttgtatttc aatcgctcgg cgcgctcaat taggtctaaa gacaataaat
    27001 ctaaaaaccc gctgcttcac tgacagaaac aacgggtttt cagaaaaaaa tgaaatgaaa
    27061 aagatctctt acggcaatct attttatttt tttgattttt tataaatgga aaatgcttca
    27121 agaagtccaa gaaataaagc cgtataaaaa caagaaccta tgaaatcttt atttattaga
    27181 tattcaacag aaatcccgat aacactagcc aacaatatat ttatcagaag tgtaaaataa
    27241 ggatgctttt tatagaatag aactagtgtt gttttcatag actctccttt cttcatattt
    27301 ccaattaatc acaaattcac ggcattacta gaaaaattaa aggggcgcag aagtggaaaa
    27361 atactcttcg actttcccta caaaccaggc catcttgtgt ttaataacta taccaaatct
    27421 aaggtacctc taaatctaaa ttccatttgt ggcggtgacc tccaattttt aactcttcta
    27481 tctcccatcc agtatacata accttgatac ttcgaccact tatatatcga acacacaaat
    27541 acctatccat tgcaggagta ctacctaact tagttgctac ttgcatctaa ataacagctg
    27601 aaaagatagg acttactctt agtaaagtat ctgaactatt aatttttaaa ctatcatttt
    27661 tatcctcaac tacatgcaca tctgttgcaa cttcactttc tgtctcatcc agataaatga
    27721 ttaaggaatc gggaattgca atatcttcag ctgacgctgg aatagttgag ctaaattagt
    27781 catcatatct ttattataaa ataaccaaca gcaaaaaata caatgaaaaa caaacttgga
    27841 actaatgtat cattaataaa tgttgaaaaa ttcagcaact cattactgta tgattttagc
    27901 agcccaacca acatataccc tataaaagct aataagatgg ttaggaatcc tttagaattt
    27961 tt
//
LOCUS       DACEUZ010000023        26588 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.023, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000023 DACEUZ010000000
VERSION     DACEUZ010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26588
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.023"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            248..1123
                     /locus_tag="IXG44_000864"
     CDS             248..1123
                     /locus_tag="IXG44_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ1657599.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(1275..2117)
                     /locus_tag="IXG44_000865"
     CDS             complement(1275..2117)
                     /locus_tag="IXG44_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ1657600.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(2209..4086)
                     /locus_tag="IXG44_000866"
     CDS             complement(2209..4086)
                     /locus_tag="IXG44_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAQ1657601.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(4083..5504)
                     /locus_tag="IXG44_000867"
     CDS             complement(4083..5504)
                     /locus_tag="IXG44_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAQ1657602.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(5507..8896)
                     /locus_tag="IXG44_000868"
     CDS             complement(5507..8896)
                     /locus_tag="IXG44_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAQ1657603.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            9153..10544
                     /locus_tag="IXG44_000869"
     CDS             9153..10544
                     /locus_tag="IXG44_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ1657604.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(10617..11264)
                     /locus_tag="IXG44_000870"
     CDS             complement(10617..11264)
                     /locus_tag="IXG44_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1657605.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(11272..11958)
                     /locus_tag="IXG44_000871"
     CDS             complement(11272..11958)
                     /locus_tag="IXG44_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1657606.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(11964..13037)
                     /locus_tag="IXG44_000872"
     CDS             complement(11964..13037)
                     /locus_tag="IXG44_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAQ1657607.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            13259..13933
                     /locus_tag="IXG44_000873"
     CDS             13259..13933
                     /locus_tag="IXG44_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1657608.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            13938..15278
                     /locus_tag="IXG44_000874"
     CDS             13938..15278
                     /locus_tag="IXG44_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ1657609.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(15427..16413)
                     /locus_tag="IXG44_000875"
     CDS             complement(15427..16413)
                     /locus_tag="IXG44_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HAQ1657610.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
     gene            17137..18168
                     /locus_tag="IXG44_000876"
     CDS             17137..18168
                     /locus_tag="IXG44_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ1657611.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
     gene            18277..19809
                     /locus_tag="IXG44_000877"
     CDS             18277..19809
                     /locus_tag="IXG44_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAQ1657612.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIPILYLMEQPALSVSQSFLD
                     KNEISYMTHTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRF
                     NIGISSLMSMGFPMMPLANAQSSKVVHMLEAHRPIALETHPNNFVQWRFTAKEHPEAF
                     SGIRYYHSTFDAINNQTMKTFLRTSMAQDAVFLQVYGQSECGPMILKAHTLESLKTSD
                     ARDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDW
                     WDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSP
                     QPIIALAPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQ
                     KS"
     gene            19948..20760
                     /locus_tag="IXG44_000878"
     CDS             19948..20760
                     /locus_tag="IXG44_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HAQ1657613.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            20750..21235
                     /locus_tag="IXG44_000879"
     CDS             20750..21235
                     /locus_tag="IXG44_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ1657614.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            21258..21803
                     /locus_tag="IXG44_000880"
     CDS             21258..21803
                     /locus_tag="IXG44_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HAQ1657615.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(21876..23519)
                     /locus_tag="IXG44_000881"
     CDS             complement(21876..23519)
                     /locus_tag="IXG44_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAQ1657616.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKHALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(23648..25876)
                     /locus_tag="IXG44_000882"
     CDS             complement(23648..25876)
                     /locus_tag="IXG44_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAQ1657617.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKERTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            complement(25909..26388)
                     /locus_tag="IXG44_000883"
     CDS             complement(25909..26388)
                     /locus_tag="IXG44_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1657618.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNMDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
BASE COUNT         7648 a         5018 c         4886 g         9036 t
ORIGIN      
        1 tttgtactct ttccattgcc aagataagcg attgtaagca tgagtggtgc ctccttttat
       61 tgatgttttc agtatatcct agaaaagcgc agaaagtatt tataatagct attctggtaa
      121 aaaagttcag aaaaacactt gttatcattc ggtttggtga aattaatgtt caaagtaagg
      181 ataaaaatca aaaagatagt agcggaacca agtgtttatc agtaagatag aatttggagg
      241 tcagatcatg cttattgaag aaaaactaac aaaacaagag ttgtttacca cgacagagaa
      301 acggattgtc gattacatcc gtaggaatat cgaagctgca gtctacatga cgattgaaga
      361 attagcaaaa gctacgtata cttcccattc agctatcatt cgtttatgca aaaaaatggg
      421 attcagcgga tttaaagaat tccgtttcga gctggggcgg gaagttcatc aattgattgc
      481 tcagttcaat caaacgattg atcctaattt tcctttttca agtgaagata gcactcagca
      541 aattgcaaag aaaatggcgg atttatcgat ccagtcaata aaaaaagcac aattgcagat
      601 tgaacatgaa gatttagatc aaatcggtaa agtattgaac aaggcacagc gaatctttct
      661 ttttgcaaaa ggtgattctc aaatcacggc aaggaaattc cagaataaaa tggtcaagtt
      721 gaataagttt ttgatcatgg ctgaagaata cagtgattct tcttggaatg cagccaattt
      781 gactagcgag gattgcgcca tttttattag ctacagcggt cgcatccatc attatgaacg
      841 aataatgacc tatttgaagc atgtagaggc ccctaccttg ctgattaccg gaaatcagca
      901 ttcagaaatg gccaaacagg ctgatatgtg tcttgttata tcgcaggagg aatatgattt
      961 tgctaaagta gcgactttct cctctcaaat tgccttcgat tacgtattaa atacgttatt
     1021 ctcagtgata tattctcaaa attttgaaga gaatatccgc aacttgaaaa acaaacaaga
     1081 attgatgcaa aaaggccttc ttaaagaata tcggaaaact taagaaaaga tcctcattta
     1141 gtaataagaa agatagtgtt tgataggctc tctttgatat catcataaaa acaggctgaa
     1201 gttttctttt tcaaacctca gcctgtttgt ttacggaacg attcgaatag atatattata
     1261 gaaatgagga atagctaact tctgtttctt attttgaacg attgacgaaa aagatatata
     1321 agaaggagga aaatcagtat acatgcagct aacaatccat acaggaaaaa tcgtttgttc
     1381 tcggatactt tctgtacttc tttttgaact tcttttgcat gataaggaac ccggtgccct
     1441 ctgacaagta agcgatgact gttgaccata taaggtgtac aagttaggag tgtcacgaaa
     1501 tcctgccctt tttcgattct cagatctttt gtatcagtcg gttctaccgt cttgatctga
     1561 tcgacttgat aagcgagtgt ccgtccattg atttcgatat agaaagaatc acctaaagtc
     1621 agtttcggta agtctgtgaa caactttgct tgaggcagtc ctcgatggct ggaaataacg
     1681 gcatgtgtat tttctccccc cgtaggataa gacgttcctt ccaataatga ggcacctttt
     1741 tctagcagca cagtattcgt ttcatcaaaa attggcaaat ggacattgat ctcagggatc
     1801 gtcaaaatac cgatcgtatg tttctcaaaa taggttctat cctttttcgc aggttctttt
     1861 ttcttagtaa agggatctgc acctggattt ccaccttctt ttgctaattg ctggtttttc
     1921 tttgtcatgt tttcttggat tttagccatg acttcttcat tttctttgac cgcttgttgc
     1981 tgataatgag agatgatctg ctgatccaga taattattca gcgcgtcgct aacaaatgga
     2041 taagctagta tccctattcc tgagagaagc aagaaaagca tcagcagatc tagaagtttc
     2101 cttttctttg gtttcatttg tgttctcctt gtgatgactg tttgataata aaagaaaaag
     2161 gagcatttgc gtaatccgct gctccttttt ccaattcttg caaaaagact aaatctgact
     2221 gtgcttgcgt ctagcaaagt acagtccagc aatcagtaga aggactactc ctgcaccgat
     2281 atacacatag attcccttac cgcctgttga aggaagtgta cctttgtgtt tatttggtat
     2341 tttttcagga gaaatcaact gtcctgctgt tacataagat tgttcatcga tagtaaatgc
     2401 tacacggttt gttaatggaa catatttttc tggcgctttc gtttcttcca gatagtacgt
     2461 gccatatttt agacctgtca catcgattaa accgttactt gtggttgtaa aaaccgttgc
     2521 tgattctttc gccgataccc agctgacggc ttttgtggat gggtcgatcg ataaatattt
     2581 cgctgtgtca ctatcttgat cacgaacgac gaattctgct ccagcaagtg tttggtctga
     2641 tgtaacgtca ccatctactt taacaaatcg tttgccccca gtaacgacat cgactgacgg
     2701 tggtgtttga tcatttgtat gcccgttatc gacatttgct tggttagaaa atcctttggt
     2761 tggatctgct ttttcgttca aatgcatata gtaaacgaat ttcaatgtac cgccaggagt
     2821 taatgaagga atataattcg gatcaactga aaccgtgaat ccgtctgttt gctcagtgac
     2881 agaataattt actgggtcga tttctttatt tccatcatat aaggcataag catacgttcc
     2941 tgaagaatca ttatcaaatg ttaaagcagc gtcatgagta tcgataagtt tgaatgttgt
     3001 gtacttgttt tgcgttcctt ctttatcagc gatacctaat gggatattga cagaaatctc
     3061 atattgaatt ttttcaccga ttgcgacatc ttttccattc aattgaggtg ttgttttatc
     3121 aactttagac gtatcatttt tgatggtctt ttctactggt gtttggctat ttgccaggat
     3181 ctcaaaaggc gtgattgttt gcttttcaat catttccgca ttatcaggag cttttacttc
     3241 ttctaaatta tattttccaa cttctaaatg attaacgatc aactggcctt tttcaataga
     3301 tgcctcggca aagtcattgt tgccgatgtc ataagaatga ccagtaatga aatgtttggc
     3361 tttggtttga tcagttgtcc aagtgtacaa tccatctgtg acactttgga tgtatttttt
     3421 gacgcttggt gttccttctt ctttagaaat aataaattct gctccattta gtgcttcgtt
     3481 ttcggcagta ccgatttttg taactttcaa cgttccatca ttaccgactg tatttttagg
     3541 gtagagatgg atagtatcta gttcttctgt cccgtattta taagagccat ctgcttgttt
     3601 gatcatctca tatacaggga aagctaaaac catgtttgcg gcagctgaca ctccgtcttt
     3661 tggttcttct ttgatcgtgt agactgcatc tttcccattt tgttttttag gtaaagataa
     3721 agtgacattt ccatcagcat ctgtcgttcc tgaagcaact ggtgtaccag gagtcaaaga
     3781 ctggactgct tgttttgctg catccacgga cgctccttta tctcgttgcg cataaaattc
     3841 ttgagtgacg ttataaactg aaaatgaaat atcggctaat ccttggtatt gatcgaattc
     3901 gctcatttct ttcccgctgt tttggattaa aggatcgggt aaatcagtca ttttcttttt
     3961 atgaaggatg acttgagctg ctgtctcctc tgcaaacact ttttttgcgc cgaagctgtt
     4021 tgtgagtaat ggtaaaataa ggaaaaggac tcctaacata acgtttattt ttttatgatt
     4081 tttcattttt tcactcctgt tctttttttg ataaacaacc agattgcaaa gcttgcgaat
     4141 accataccaa caacgcctaa aagtgtattt ttcgtttcgt ttgtttttgg aagaaatgga
     4201 cggctatttt cttttttatt gactactttc aatatactgt ccgcctcttt atcctcagaa
     4261 agaaaagttg aaatggtaaa cggaatttct gtttcactta aaatataacc agaaggtgct
     4321 tcgatctctt ttaatcgata acttcctgtt ttcaacccgg aaattgaaaa agcgccattt
     4381 ttatcggaaa tcaggtgaag cgcatctgat tcgttctttg tccacttgta tccgttggct
     4441 gtttgctgga ggtaatttcc tgctttgttt ttaataagga atactgcgcc agctaatcgt
     4501 ttatctggtt tatttgtctc gattttgaca aattgcttac tccctgtata tacagagact
     4561 ttctttgtac tgacaccaaa accaaaatcc agtgttccgt tgttgttgat ttccttgttc
     4621 gcctgtgctg tactgcttag ctgcatctga taagaaatgg tcagtttttt tccagcatat
     4681 ttttccaacg aagggatcga aaataaaaca tcaaatccgt gaggtttctt gtgtatgaca
     4741 aagccttctg tcagcttttc tccattcgat gaaatcgtta aactattagg taaaaacgtt
     4801 aatgcttcat ccgcactatc tgacaattcg aacttttgat agtcaaggat atttacagga
     4861 attgtcgtat gcaaggaata agtgattttt tcaccaatcg taaaatcatt tctaggctct
     4921 tctaatacct tctcaaaagg tggatcaggg tagtcgtttt cttcattttt aggatacaaa
     4981 tggatagttg aaagtttttg attgttttgt ccatgtacag gaagaacaac taccatattc
     5041 tctgcctttt ctttgacgac ttctggtact ttggattcaa tgaaaagata cgctttatct
     5101 cttttttctt gatctttagc ggccagagaa aaagaagcga taccatcttc gttgttaaga
     5161 gttgtagttg ttgcttctgc ggtaaacata ccggaagacg caccgttttt tgcgatctct
     5221 gcttgtgctt cttctaccgt tttgcccttt tcccgtagat ggtaaaaaga atctgtgaca
     5281 tcataaactt ggaatgtgac accatttaat cctcgatacg tttgtaataa agctttttct
     5341 tgtccgtcat ttggatgatt tttcggcagt tgcccgtttg ggaaaagcaa tttgtgtaaa
     5401 gtaatctgaa ccatttctgt ttctgtttct gttgctacct gagtaaaagc tggtttaaat
     5461 agtaacaaga agagacacat actaagccaa ccaagttttt tcatatttag ctgccccctc
     5521 tttctttttt caaaaaaaga taccaaatag aaaacgcaca accaatcatc cctactagat
     5581 agattcctaa acggccaatt cctcccgttt caggtaatgg tacttttgcc tgattcgtga
     5641 tgtctaaaga aatctggttg tttttggctc ctggtgacag aacagattca tgatcttgtc
     5701 catccacttg aattgaccca tcttcgtgta taactatagt aactggctct tttagacctt
     5761 ggtatccttc tggtgtaaaa gtttcggtca gcgtataagt tccaggtttt aaattttcga
     5821 atagaaaggt atctgtttct tgtccatctt ttggcgattc taagtccatt tctggtccct
     5881 gcagtctgaa ttttgctcct tttaatgtct gtccgttgtc ttcttttttg tgtaccgtta
     5941 gatcaaacgc ttttagtcga ttttgatgag tgagcgtcca tacattattt gtcatctcgg
     6001 tattttttcc cgtgtaaatg atttttccat atttatcgat ttggaattca taatttcccg
     6061 gcgaagtatc atatccggca ggaccagctg tttcaacgac tcgatattta ccaggtgtga
     6121 gtgaatcaaa actggcaaga cctttttcat tcgttgtttg gctgtcaatt ggctgataag
     6181 taccatttgc ttcttttttc tgtagggaaa atgctgctcc tttaagattt atctctttgt
     6241 ccgtcccttg catcgcatac ttacgtactg ctacaggaac ttcaacaaaa ggattttcaa
     6301 tagtcaactg gatcgtatca tcggaagtag tgactgtttt tccatcaatg gttaccgtac
     6361 catcagaagc gatcttgatt tcccaagtag tctttttgct taatccatgc ccttctggag
     6421 cttttgtttc tgtcagcgta taggtcattt ctttttgcaa tttgacattt tctggaagag
     6481 aataggttcc gtcgccatga tctgttagta aagtatcaat agaatctcct gttaatttga
     6541 aaacagcgcc aataagatcc ttttcacctg tagaggaatt tttcgttatt ttcaagttta
     6601 acggtgtgaa ttgtttagta tttttccatg tcattgcatc tatttgttga gaatcgtatc
     6661 ctggtacagg taattctttg attgtttggt aactgaatgc ttgaccttga ttattgtatt
     6721 gaggtagtga taatatctct tgataacttt ctccgctatt tgcagataat ttgtcaatgt
     6781 ctgcacgttc ccacgtattt gttgtatctt tagctggttt aatgattcga atatatccgt
     6841 ttttccaagc tgcagcattt gtcgtatgtt cccgttgaat ctcaaaagta acttgatctg
     6901 gacgatcagc tagattgttg tcaaattctt cccataactt tttgatgtga agactgactc
     6961 cgggagcttt agcagatggt ataccgaatt cagctaattc attggtatca atgtttggct
     7021 ggaaagttgt cctgccgttc atttgatacc agaaatttgg atggaagtcc tcattttctg
     7081 tttggattct cacttggtaa tggatttgga tttcttggtc ttttcctaaa tagatctgat
     7141 tgctcttgat ggtatttcct tctatggaaa tagatggagt gaccgttgta ggacttgtcc
     7201 ccacactctt gactgaaagt gtaccaggct gataaatgaa tggttctgcg attggatcat
     7261 ttatttgtcc attgcttaca gttgctgaga tctgtacagc ttttttggct aggtattcag
     7321 agatatctgt tgcatgatca gctgattcat agtaaagatc gcctttttca tctgatgaaa
     7381 ccatttgtcg catacgagac tctacttctg cttttgagag accagctgcc ggatcgcttt
     7441 gaagttggat gccaagacca tgtatttcga ttcctcgttc tttgagtgcc atcgcttctc
     7501 cgatggttgc gataaacgta ctgtcgattc ttctggatag attgttttgg tcaggtgcat
     7561 catagattct tgagattaga gaagtatttc ccggccgatc ttgcgtatta gaaaactgag
     7621 ttccgtaata attgctgctt gattgtgcgt gtacccgctg tactttatag gaaaatgttg
     7681 gtacaccatc cgtcagcaaa acgatcactt ttttatgtcc atttggaacg gatagcatgc
     7741 ttcctgcatc tcttagtgct ttttgtgtaa aagtaccacc atttgtccgt gaaggtgtaa
     7801 tggattttac ttgatttttc actgaatcaa atgaacccat ctgtactgca ccgttactgt
     7861 agctatatcc ttcgcttgag tagccgacat atcccatatt gattttgtct gtgataccgc
     7921 tatctgctaa agtatcgaca aaacgatcga caccaatctt tacttcaccg atccgattat
     7981 tgtcgttcat acttcctgac cagtctacga ccaatacgag atcaagaggc gtgatatctt
     8041 tttgtacatt tccacgagca ttcaaataaa catcaaacaa tccgggtgta cttgtttcct
     8101 ttgcatactt tcgtatcgca tagtctgctt gattaccggt tcctccatat tcgatatacg
     8161 aatgggtccg atcattagga tctccattcc aagaatttat accatcccat tgatttgttc
     8221 cgtttttatt gccttgatga tcaagaacat ttgtatttcc tgtaggttgc caactggctt
     8281 taggataagt gccctgatca tctgttgtat attctgtagg aatcgtttga ttagatggca
     8341 cactcacagc tggcaaggat aatgtaggca atgtaaatgg gctgacagaa ttttcgtttt
     8401 cttttgatga ttcttcttct atttcgtctg gctttggtac tgacacatct gtatctttct
     8461 tattttctgt ctcatcaata atgtcatatt ctttattatc gacgatcagc ttgtttgcct
     8521 ggctgtggta aagtgctgga tcgattggaa aactcagatc gatagtttgt ttagtgtttg
     8581 atgagatttc caacgtaatt ttcccgctat ttacctgata agcatagcca tctccttctt
     8641 ttctttccac atcacttaag taattttcga ctagaccaat ttctttttct atcttttcag
     8701 cactggtgtt attcgcattt agcttgatgt tcagtgcttt ttttccagac tgatccgaaa
     8761 tgacttcagc tgaaattgtt tctaaaactg ttggatcatc tgtagtgtct gctattgcag
     8821 ataatggcac aaagtttgat aaactcaata ttatcagcat gaaaataact ttcagtttct
     8881 tccctgtggt cgtcattttt cccctccttt ttcttttttt ctgttttgac gtgaatagtg
     8941 aaaattatta aaataaacag gcgttacata attttattta ttttaatatg ataattatta
     9001 tacgaagggc gctaaaggga atcacaccaa ttttcatacc aattggtttc atttatgaag
     9061 ttgattcctt cgattaatga gcgtttcatt gaaagaaaag ggttgaggga gaaaaacata
     9121 tgatcaggtt gttggaatta aactatagaa agatggaaaa aatcattcga attctgtcca
     9181 tttctgacaa gtggatgact aaaaaagaac ttgccaaacg aattggtagt tcagaatcta
     9241 catttgtaag gtatatcgaa gagatcaaaa tccgctgggg aacggatgta acgattcaat
     9301 cttcacaaaa attggggtat cgtcttgaac atttgaatgt accagcttat ttaaatgttc
     9361 tgatggaaat gtcgcaaagt tctacaaaca gtcgtttatt aaatgagatt attgtcaatc
     9421 cgggtaaaac gattgagtat tattgtgaaa aaatttccat cagccggtct tcttttacac
     9481 gtaagttgaa acaatgcaat caaattttag aaagctattt attaaaagtg atcgttgacc
     9541 aaggttttca gttaactagc cttaaaagtg agctccagtt gcgtatcttt gttacctttt
     9601 tctttttaag ttactacgga agacgcacgt tgccatttcc aatcgaaaaa aagaagatca
     9661 aagaaattat ggtcaagaat aattgtcaga tgaatttgtt tcaggaagaa agcccatatg
     9721 agcaatcttt ctttattatg tattttgttg tgcatctcat gagagaatcc caaggaactc
     9781 catttgaatg gaaagctgga gactcttatg tacagttgat aaatatcgat aaaaaagact
     9841 tcttaatctt gaaaaatctc ttaggaaaat tgaagtacag cagctatata aaagtagtga
     9901 actattttgt caatacgatt ttttcagaat tttcggagaa agaaaaaaat gaaatcagtg
     9961 agcagatacg aaaaaatatg tcctactgtt cgttttcatc cgctgattgc aaagaaaaaa
    10021 acgaagaata tgttgttcaa ttattaacgc aaatttattt tttctcccgt attttcccat
    10081 acgatacgac tcatttgacg atccgtgcaa cttactttgc acagcagatg caacaccgcc
    10141 agatacgtct atatgctgga tttcgtgcag aacttcaatt ttattccact ctactgaatc
    10201 agaatctgat ttattattta tctagctata tcttttggat gcttatcggt aatcctgaaa
    10261 ttcaccatta tacttctgat aaaaaaatac tgattatcag cgacttaagt cttcgacata
    10321 gtcaatatat tgaagaatat atcagtgata ttcttgctgt gcataaaatt cactctgaga
    10381 caactgctat tactgaagat cagctgtcga aatacaatct tctcgagtat gatctgatcg
    10441 ttacgaatca acccattttg aatgcccatg ttcctcatat attgattgat gatagtgtgt
    10501 cattcgcaaa tgaagaagaa ctcatccgct tattcgagtt atgaataatc aaaaaacagc
    10561 tatgtataaa ccaccctgat aaaaatttca ggacaaatac acatagctgt ttctttttat
    10621 tgtattcctt cagcaatcca acgagattta tagcgaaagt cttttaacaa ctcttctttt
    10681 gtccaattat ttgcaatgag atccgtcaaa gtgttcatta cgagggtcat ggaaaagtat
    10741 aggaaactaa ctgcttcttc cgacgattga atggagaaat gattgattct taataattcc
    10801 agccattttt cgttcattgg agaatcagcc gatagttctc tacgtttggt tgtctgctga
    10861 tcatttcgct gggttaacaa gtataacctt tgccaataga tactttcgtg atctaagtcg
    10921 cagcaccact ctaaatattt ttcgatcccc aaaaagaaat gttgtttgtt ttcgctaatg
    10981 aattttaaga tatcttgatg gactatgtga gaactgcttt tgatcgtata caaataagca
    11041 tcttccaaat cttgaaaata tttataaaaa gcaccgcgcg acatccccat cttgtcaacg
    11101 atttctgaga ctttgacttg gctgatgtgc tgtgagcaaa atatctccag taataggtca
    11161 tcgattctct gtttctttgc gttcgataaa ttgaaaaaag tttcttttgg catccacatc
    11221 tctcctttat ctctgattat acgtggatag cccttcattg tcatgaagga ttcattcctt
    11281 ctttttctct tcttctgtca agtacccatc ttccattcga taaacgcgat cactccattg
    11341 aatcatacgg acatcatgag taaccataat agtcgctttt tgtttctcat gagcttcttt
    11401 aactagtaat ttgacgactt catatgcatg atctgtatcc aaactagctg ttggttcgtc
    11461 tgctaagatc aagcttggct cattatataa agcgcgagca attgccactc tctgtctttc
    11521 tccaccagaa aggtctttag gataactatt tttaagtgac tggatatcta aagattgcag
    11581 caaggaatcg atttttgttg aatcttcttt ttttgaagta acttggtcaa ctagttcaaa
    11641 ttgttccttt accgttaaaa aaggaatcaa attagagctt tggagaataa atcctatttc
    11701 tttaaagcgt aaatctgctc gttttttttc tggaagctca gttagtgagt gaccgttgat
    11761 cactatttct ccggaagtcg gtgtttgcaa tcctcctgaa atggtaagga aagtactttt
    11821 acctgaacca gaaggaccaa tgatgctgac gaattctcct tcctttacat aaaaatcaat
    11881 tgctttcaaa gctgagacct ttgtatgacc agaaccaaat gttttggtaa cttttttcat
    11941 cgttaatatt tttttcattt ttattaacct cctattgctg taattgggtc tactttggtt
    12001 atcgtacgta tcgaaaacaa tccgcctagc attgcgacaa tgatcaaaat cccactatat
    12061 agcaaccatt gtgaccaaaa aacagcaaaa ggcatcgcgc ttggtaaaat cagactagtc
    12121 aaataagcaa acaaaagagc aagcagaacg cctaccaccc caacaataaa ggactgtgct
    12181 actaaagatt tagcgatgaa gaaatttttg atcccttgtg ctttcattac tccaaaaata
    12241 gcagtcttct gaagtgtcat cacgtacatg aaaataccga ccacggcagc aaccactaag
    12301 aacaaaaagt atatcatcgc atttaaggtc atattttggg cagaatagcc aggaatactt
    12361 tcaatgaatg tcccaatagt taataactgt agacccttcg atgctttttc tttcgaaatt
    12421 ttagctgaat cctttattac gatcccattg atcggctgtt cttcttcaga agcaaacggc
    12481 tgttgtccat acttcaaagc agtccacgta tctagggacg catagatgac gggagaaact
    12541 gtgtaataag tttcaggaaa gataccgaca atcgtcaaag gatcgtcgta actgccaatt
    12601 ttgatcgtat ctccaatttt atagcctgaa tcagccagat tttgagagat gatgatttca
    12661 ttctttgttt taaattcatg ccctttcgtg agttttggta ataaaaatgc ttctttcgtt
    12721 gtgccgaaaa ctgtaacatt ttctttgttt tttcctttga ttgctccgct gtacaatccg
    12781 attggtgctt tctctcctcc ttcaatgtat ttcagatctc ctcttgttaa ttgtgaagct
    12841 gcaaatactt gatttgcatc ctccgacaaa acaatctctt gtgcgtccca atcatcgatt
    12901 gcttttttaa actcttctga aagtccattc gctagtcctg acaacatgaa tacgacatac
    12961 gcaatcaaga acataatacc gacgatcaag ccatatcgta atttagaata gcgaatttct
    13021 ttgatagcta gaaacatttg tttttcctcc ctatgataga ttgatagtga caaaatgtca
    13081 cttgttgaat agtgtaactc taaagtgaca atttgtcact tgtgttgttt ttttcttatc
    13141 aataaattgt ctaagacatc tttcgataaa accttaacat gagtcataaa aaatttattt
    13201 aagccttatt tatgctttcc atgataaact aaagtgacca aatcaatgag gtgggaaaat
    13261 gattaaacta ttagtagtag aagacgataa agtattatcc aataatataa aagaaatact
    13321 cgctgcaatc ggcgagataa cacaagttta tgatggtagt gaaggactgt tcgagttgga
    13381 aagtggagtt tatgatcttg caatcttgga tttgatgatg ccggaaatga atggatatga
    13441 agtgttagca aaaatacgga aagaaaaaat acagactcca gtattgattt tgactgcaaa
    13501 agatagtttg gaagataaga ttgaaggatt tgaacgaggt gcagatgatt atctaacaaa
    13561 acctttctat cgagaagaac tgatcatgcg cgtgaaagca ttattgaaac gctcgctagg
    13621 tctctttgat gaaaatgttc tgaagtataa ggatatcagt tgtgatttaa aaagcaatga
    13681 agtcttgtat aaagaacaag ttttgcccat acaaggaaaa gagtttgaat tgctcgttta
    13741 ttttttacaa aataagggaa tcattttgac aaaagagcag atatttgacc ggatctgggg
    13801 atttgattct gatacgacga ttacagttat tgaagtttat atgagcaact tacgaaaaca
    13861 tctgcgaagt actggattgg ataaagaaat caaaacctta cgtaatgtcg gttatatact
    13921 gcaaagggtg tgatgcaatg aatggagata ttacacgaaa gcaacgtagt cgcttttttg
    13981 cgacaaatgt agcagcattt gcttttatct tttttctttt gggattcatt actttacaga
    14041 tattaaacca atctgcatac agagaaacag atctgtcatt agagaaaata aattttgatt
    14101 ctcgaacagt acaattagag atacaaagat atcagcaagg cgatcccttt ttaaacccga
    14161 ctcaaacgga acaaaacaga acaaacatgg aacagccatt aatggaacca tcaaatgacc
    14221 gttttaacac tcaagtgatc ttgtggtcta aaaatggtga gatattgaat aaagcagcta
    14281 ttggtgggcg tttcaaccaa atcaaagaat taaaaatgga tacgaaaaat ctgaatacca
    14341 tccaaaatat tgaattaaat gaagacagtg gctcatctgc cctctctttc cgttcgatta
    14401 cgaaaaaagc ggagacgaat caagatggcg ttgcctacat ccaaatttta gaaaatacaa
    14461 atcaaatcaa gaattcatta gcgacttttc gcacgattat gattttgtgt atgattgttt
    14521 tttggattct ctctattggt atcagttatt acttgtctag tctaagcatg cgccctatta
    14581 ttctttcttg gagaagacaa aaagaatttg ttgagaatgc ttctcatgaa ttgcgcacac
    14641 cgttaacgat tattcaaaat agtttggaac atctttttac caagcctaat catactatta
    14701 ttgaagaatc cgaaagcatc gctcaagcat tgtcagagac gaggcgcctc actggattga
    14761 cttcggattt gctgacaatc gctcggaatg attctgatca gcagctgctt tcaaagcaaa
    14821 tgatcaatac acaagaatat atcgaacaac ttgttaaacc atttcaagag atggccatca
    14881 tagatggaaa agagtttatt ctccataacg atgcaacatt agaagtacca attgatgaaa
    14941 aaaaaatcca tcaagttttg gttattttgc tggataatgc attaaaatat acgaatccag
    15001 aagataagat cattttggat tcatctgtta accataaaga atggctgatt cacgtaaaga
    15061 atactggccc ttctatcagt gaagaagatc aagcaaggat ttttgatcga ttttatcggg
    15121 aagatcaatc tcgggcgaaa gagactggcg gatacgggct tggcttggca attgcaaaac
    15181 aggtaatcga acagcatcat ggacagattt ctgtatccaa ttatcttcca caaggagtcg
    15241 attttaaagt ccaattgccg ttgaagccta aaaaataatg cttatcccta aaaaagggct
    15301 gtgacaaaaa tcttgtcaca gccctttttt gttgcgaata aacggtgttc cagaagtagc
    15361 ttcttcggaa ataagccgaa atttctttaa gctgaccact tctgtcacaa cctctttagc
    15421 ttacgcttac gattttttat tgcggtcttc aatgtgtaca tcaccgcttg tgtcaatatc
    15481 caattcttct cggctaacat cttctgtcac tttttcaaca tcttcatgtt cttctttatg
    15541 aatcccaact tcttcacgaa taactgtatg tttgcttaca tctacttgct cttctttgat
    15601 tgggatagta atcgtgtcat cttccaattt ttcgtctgtt ccctgtgaag attgatctgt
    15661 tactggtttg cgttcgatga cgatttcttc acgtttgacc ggtacttcta ccgtttcagt
    15721 atcgttgacc acatgtttgt ggatgtcgac ttctccagtt gtcacatcat gtgtatttac
    15781 atctaatttt tcttctttta gttgaattgt atcatcatct agatttggtt tgcgatcgat
    15841 gtcttcagtc ttgcttgtac ccattgtatc agtctcgcca ggtacgtcca tcggatcagt
    15901 tcctggtgtc atattcaatg gatccgttgt attttgaaag cctgtcgtgc cacctacgtc
    15961 cattggatct gttccagtgc cagtttctaa tccagtcccc attgtgcttg ctgggctaac
    16021 agtatcttgt gtatccattg tatcttctgt tgtttctggc tgacgatagc cttttactgt
    16081 gatgacaaat tttccatcag aaatatctcc tctatattga tataaaggat cattttctgg
    16141 tgtagccgtc tcagaatcat aggtatcaaa agaaaaagta tcttttattt tttcccataa
    16201 agaacggtct tcttcaccat ccatccgatt ttcatctgtt tcaacatcta taccagacaa
    16261 tccgtcaaat cctagtctag atgctgcttc atcagtagta taaataacga tatcatctcg
    16321 ttcataccct tcgttacgca accgttcaat tactgataat gcttcttccc tcgtactata
    16381 gcttccgatg accgttaaat ttttctcttc catgtaaatt cctcctagca aatagattta
    16441 tgattggtat attgttactt ataaatttat tctataggag acgattgttt tctacaaata
    16501 tttagattgg aatttgatta aaacaattta attgaaattt gaaaacattt atgttaatga
    16561 aagtcatatt ctaataaaat gtcatttgtt tctatatgaa tttttatgaa aaatttcata
    16621 taatttccac tcgttgtgat ggtttaattt atccaatcca tcaataagca aacaacttat
    16681 ataaaaaagg attaattctc aatataattg ctcctaccat agaataaatc ttcatgtaaa
    16741 gtaatgaagt accaactatt tctaatactt ctttaagaat tatcccaaca atatcgttag
    16801 acaaaccatt attgaataaa tggagagcca atcgattctg aaaaaatgtt ttctaatttt
    16861 tcggaaataa aagaaagagc ctcctgccat tagaacgaac caaatccatt tcattgctca
    16921 tgaagtaacc acatagctaa aaagaggata aatcaattaa aagatcaaaa gaatgactgc
    16981 actaataaaa taactaaaaa tgtatcttct tacttcatat cttttagcta gtacttagtt
    17041 aaaaatttgc ttcctaagat caagacaaac tgtctatctt catttgatga aatgcggtaa
    17101 aatatagaaa atgaacacta ttggagggaa tttgtaatgg acagcaaaac tagaaaacaa
    17161 ctgcaagaaa agttaaagca acgtacacac ttgctccctt ccgtctttca acagttttta
    17221 gaagaaaatc gttcttccct ttcattaggc agtcgctatg aatacgcaaa agattttcat
    17281 ctgtttactg attatttgag aaaagtattt tctgaagaag tggatgatca gctgattatc
    17341 catttagaaa aacaagatta tcagaatttc ttagctaaaa tctatcgaca tactcgttcg
    17401 ttccaaacta cagcgaacca agaaacggag caattgtttg aaaatagcta ttacgggatt
    17461 tcaaggaagc aatattcatt aaatcatttt ttacgatttt tgtatcaaaa aggactgatt
    17521 gaagaagaga tttctctttt gggtacaagt ttaccggaaa caaccaaagc agctccgcga
    17581 tatatcgatg cagaactttt caaagatttt gagcatttgt ttatcccgat tgaaggcttt
    17641 caaacaagca gagaagctag ctttgaagaa aagaatagac cgaggaacct tgcaattact
    17701 gccattcttc tgtattgcgg attaaagatc catgaagtag tagaacttaa acgtaaagat
    17761 gtatctttga ccaagcagat tcttaatctg aatagaaaag aaaacagact gaataaagta
    17821 ccaataccaa aagaagcgat gccatttctg gagaactatt ttaagttaac agcacaaaat
    17881 acggacgaaa attcttttgt ttttcgttcc tcacatgatc agcaaatttc tcctcggact
    17941 attcgccaaa ttttgagcaa aattacagta tataaaaacg tatcgccaga actatgcaga
    18001 aaaacatatc gttattatgc agaactatat ttagatgatg ctgacgcggt aaattattta
    18061 tgtggtaatc gatacctctc atcccattct tttcaagata tctgggaaaa gatggttgat
    18121 ttttcttatc acgaattagc ggcattcgtt cgggtttctt ccatataaaa caaatgtatg
    18181 atcttttttt cacaataaac tatgttatta tataaaggat tatgttacga ttatcttgaa
    18241 tttgtatatt acttaacaaa agataatgga gtgtgaatga tggagaccat aacgtattcc
    18301 cctttgaact tatttacgaa ttttcaagaa gctgcagcta acttttccga gaccccgatt
    18361 atctttgatg agccactttc agctttccca gaattaggac ttgaaacaac ttacaaaaaa
    18421 tgctgcgaaa gtgtgttgaa acgggcttat caattggctc atcttggagt gaaatccgga
    18481 gacaaagtta ttatttataa gagttccgct tttgacacat accttctagc tgtttctgtg
    18541 tcttaccttg gtgcagtgcc tgtcatgact tcttatcatc taccaacaac aacaatggaa
    18601 gtatttatcg atcgtttaga agaccctttc attttatttg atgatgagac aaaaaagcga
    18661 gtgagagaaa tttcaaatgg aacaaaatca aaacagattc ctattcttta tttaatggaa
    18721 caacctgctt tgtctgtttc tcaatcattt ttagacaaaa atgaaatttc ttatatgacc
    18781 catacttctg gtacaactgg tatccctaaa cttatttgtc attctgctca ttctatggga
    18841 tggcggacga aatggcaaaa aacagttttc acaaaaattt cggaaaagaa attaatcgct
    18901 tttcacatct ctccagtgca ttccagattc aatattggta tctcttcttt gatgtccatg
    18961 ggatttccta tgatgccttt agcaaacgca caaagttcaa aagttgttca tatgttggaa
    19021 gctcaccggc caattgcact agagacccat ccgaataact ttgttcagtg gcgttttact
    19081 gctaaggagc atccagaagc tttttcgggt atccgatact atcactccac ttttgatgct
    19141 ataaacaacc agacaatgaa gacatttttg cgaacgtcga tggcacagga tgcggtattt
    19201 cttcaagtat atggccaaag tgaatgcgga ccaatgattc ttaaggctca tacactagag
    19261 agtttgaaaa caagtgatgc aagagatatg ggcgttggac tggaagacat gacaagcgct
    19321 cggattacag attcagttgg gaatgtccta cctgaaaaca cagatggtca tatacaatta
    19381 ctgtctaaag gacgggcttt gacttattac aaggaagatg cacggttcca ggaaaatgtt
    19441 tatggcgatt ggtgggatag cggagattac ggttttatgg ataagcatgg ccacctattt
    19501 ttgaaagatc ggcaagtgga tttgatcgaa acgatcaata gtacattagc tattgaagac
    19561 tttttattag attcgttaga ttttttggca gaagtagtga ttgtaaggga taaaaatcat
    19621 tctccacaac cgattattgc tttagctcct gataaagaaa tggattggaa tcgatggtgg
    19681 gagcaagtcc atgaacttcc tcgcttgaac gtccctatca tccgtgattt tgatgcaatc
    19741 ccgcgaacag ctacaatgaa agtgcaacgg cttcaaatcg agaaagaatt aaaatcacaa
    19801 aagtcctaac tatttaagga aagaaagatc cattttggtc tttcttttct ttttgttttt
    19861 ttaaattgga tgagataaaa atatgccaat tggctgtttt tcttttttga aaatttcgtt
    19921 atactcttat tcatggagga aaatgttatg aaaagaatct tgacaattgg cggttctgat
    19981 ccatttgctg gtggaggaat ccaaacagat ttaaaaactt ttgagaattt tggtctattc
    20041 ggactaagcg ccttgacttc tataggggca ctagatatta atgagcgatt tatgctggaa
    20101 tctatatctt tggaattgct taaacgacag ttagtttcga ttgataaaat gacttcatta
    20161 gatggtatca aaattgggtt attgaattca agtgaagcaa tcatggttgt tcgagacttc
    20221 ataaaaacaa aacaaaatat cccaatcatt ttagaccctg ttttggcatt caaagaaact
    20281 gaagctgtga tcaatcaatt gtatatttcg cagctcatcg aagaactttt tccacttgtt
    20341 acgatgatta cgcctaattt ggcagaagct gctttacttt ctggtcaagc tcttccctct
    20401 tcgttaacag aaatgatcca tctcgctaaa gaactcactg cttcaggaac aaaaaacatt
    20461 gtgatcaaag gcggcacaag gatggctgga gatgaagctg tggatcttct ctattcaatt
    20521 gatcattatg aggtattttc tcataaaaaa atcgacacta caacagtaaa cggggccggt
    20581 tgtgcctttt cttctgcaat aacagcgaat cttgtgttag gggcagattt taccaaagca
    20641 gtagcagaaa gtaaaaaatt tgttgcccat tgtatttcta atggcgtgct gatgaacgat
    20701 cataccggaa gtgtctggtc tggcggtatt caaaaaggag gaatgagaaa tgaaaactaa
    20761 gcaattaacg atctatgcta tgctgattgc gttgacagtt gccctttcat taacaatttt
    20821 gatccctgta ccggctacga atggatttgt gacattatgc gaggccggga tctataccgc
    20881 tgcttttctt tatggtccaa cgggtggatt agttgttggt gcttccagtg gtctattgat
    20941 cgatttgatt tcaggatatc cgcagtgggc agttttttca ttagtgatcc acgggttaca
    21001 aggatatgtt gctggttctt tcagcaaatc ttctactcgt atgtggggat tagggctttt
    21061 attaggaagc ttagttatga tcatcggcta tggtatcgcc ggttggattt tatatgattg
    21121 gccggctgga gtggcttcta taccaggaaa catcttccaa aatctgattg gaatgggact
    21181 tgcctttcct ataactgcaa gtcttcaacg tattcaagca cggaagcaat attaattgaa
    21241 aaagaaggtt ttagaaaatg aagttgtcag aaaaagaatt aaaagaccaa ttgactgaaa
    21301 tagttaatga catattggct gaggcacact tgaaaaaagg tgatcttttt gttttaggat
    21361 gcagcacaag cgaagtcgtt ggaggacata ttggaaaaaa ttccagtgcg gaagttggtc
    21421 aatggatcat ccgtacgtta aaagagttgt tggatcctaa agaaattgcg ctagctgtcc
    21481 aaggttgtga acatttgaac cgagcattag tagtagaacg aacagtggca gaagcaaaaa
    21541 atttcgaaat tgtctctgtt gttcctgcac tacatgctgg aggtgcttgt tctgttgctg
    21601 cttttgacca gttcaatgat cctgtcgaag tggaacacgt agttggacaa gccggtatcg
    21661 atatcggtga cacatcaatc ggtatgcata tcaagcatgt ccaagtacct gtcagacctc
    21721 gtttgaaaac ccttggacaa gctcacgtga cagcccttcg ctctcgtcct aaatatatcg
    21781 gtggtcctag agcgaattat tgaaatgagt aattgacaga gcaatctgaa aaacttcctc
    21841 gcaaagtcca actaatgaac tttaacgagg aagttttact tgtttgtttt atataaccga
    21901 gcttcataag gtgctagtat gcctttagct tcaaattctc cgctagagga atttgacaag
    21961 agtaatgtcc aggactgatc tgcttctttt ggaagttcat agtcagcaag tgattctgtt
    22021 aagtttacca taataagata ttgttcatct tctaaatatc tttcatagac ataaagttgt
    22081 ggatgttcat gaagatattc tttataagaa ccgtatatca gacctttatt ggtttgtcta
    22141 atgcggatca tgttcttata gaattgcagg accgactctg gttcatattc ttgttgttct
    22201 acattgatcg ttttggtatt tggattagtg atcagccaag gttcatgtgt agaaaaacca
    22261 gcatattgtt ccgaagtcca ttgcatgggt gtcctgctgt tgtctctcgt tgtttcacga
    22321 atgatatcca atgcttcttc tcttgtctta ccttccgcaa gcagcctttt ataaagtctt
    22381 ttcgaatcta ctgcatcaac ttgatcgatc gattcaaagg gcatattcgt cattccgatt
    22441 tcctgtcctt gataaataaa tggcgtacct tgcaaaaaga aatacatcat agcgattgct
    22501 tttgcagaca tagcccaaaa atcgggacga gtatctccaa acactgaaac ggcacgagga
    22561 acatcatggt tttccatata taaagcgttc catccgatac catccaatga agtttgccaa
    22621 gcgcttaatg catgtttcaa tttaaggaca tctaattgcc cttctttttc ttgctgccag
    22681 atatggatat gatcaaattc gaatatcata tcgaaataac cgtctttccc tacccattgt
    22741 ggtccttctt ctgcagtgac accacttgct tctccgacag tcatgatatc gtactcttca
    22801 aaaacatgtt tcaaatcggt caaatgttct tcgattcctg agacattttg aaagggtgag
    22861 aaagggttct ctgtggcttt tactgaatat tcatcttttt ttacatgaga aatcgcatct
    22921 aaacgaaatc catctatccc tttatccaac caccatcgga tcatattgaa tagttcttct
    22981 ttcaatttct cgctttccca atttaagtct ggttgttctt ttgcaaaaac atgcagataa
    23041 tactgtccag tttcatgaga atactcccaa gcagaaccgc cgaaaatcga tgtccaatta
    23101 ttcggttctt tgccatctac cccgtcgacc caaatgtaat aatcgcgata cgggctttct
    23161 ttagattttt tcgattcttc aaaccattga tgctgatccg aactatggtt gatgacaaga
    23221 tccataatca ctttgatccc taattcatgt gcttctttta gcaataaatc aaaatcttcc
    23281 atcgttccga atctctcttc tatatcttga tagtcactta cgtcataacc attatcgaca
    23341 aatggagatt gatagatcgg attgatccag ataaagccga taccaagatc tttcaaataa
    23401 ttaagttttt cacggatccc attgatgtct ccttgaccat cgccattgct atctttgaaa
    23461 ctagcaggat agatttgata gcctactgct tctttccacc atttttgttc ttgtatcata
    23521 tcgtttcact cctttaacgg tccctcttga ttataaccga ttctatttta gaagtatcct
    23581 tgttaccggt aactagaaac gaaaagctca acgaatctgc taagtgcgtt cgttgagctt
    23641 tattttatca tagatagcgg gcaatcagtt gaatcatttg cttgggaaat tcattcaagt
    23701 cagtaatatc tacaaaacga ctttcaccgt agatcgactt gatttcttct ttgtcttggc
    23761 caatagctgc ggagacaaat aggattcctt gacgttcata ttctttcaaa acagactgga
    23821 tatcttcttt tgcttttttg cctgtataat ccggcaacgc ttttggctga ccgtcactaa
    23881 tattaagcaa cagtttggtt gttgcggatt gtctgtttaa tttttctgct gctaaacgaa
    23941 gtgcagcacc atcacgattg ttttgccttg gtttcatagt gactaatttt tcatttaacc
    24001 attgaaatcc gtcttcaaat tctttataag aaaacaaaga agtcttttct cttgacgaac
    24061 ggtctgctgt atctccgtaa atcaacaatg ggatatcgac tttcttagaa aattcggcaa
    24121 tggcaatcgc tgcttttttt gccgcttcga tccgatcatc ccgtatcatt gacccagatt
    24181 catcgatccg tacagctacc gctaaagaag gttgctcatg tggcgggttc tttttgtcaa
    24241 aatttctcaa atcaccatat gctactttgc ttgcattgaa ccgctgacct tcgtattttc
    24301 ctttttggta cgtctctgtt tgttcattat ccagtaactc taaaatttgc cggctaagtg
    24361 attctactat tggcattact tgcatgtgta gctcacgtgc ttccggcgtt tttccttgga
    24421 aagctggtcg gtgaacgatg agaccttctt tttcatgaat cgtttccttc atgatttgtc
    24481 gcgcttcttt attcagttgc ttcttgattt ccttatcatg tgcttccatg tcagaagcag
    24541 acatttcttg agaatcatct cctgtttgtt gttctttttg attttttcct tcgatatcgc
    24601 tactgcgttc tttttgcatt tgaggaggaa tctcgttttt ttctattgtt tcttcttgtt
    24661 tctcttctcc ctcatttggt tcttcagcag acaacgattt cttttcatct aagcgcagaa
    24721 tttcttcagc attttcagtg atgtttcgtt catttgcttc ttcggcgcta atggttcttt
    24781 cttttgtatg ctcagattga ggatgacccc atttttcttt tctcaatatg tcctgaagtt
    24841 ccagtagcaa tccactgtca gacaaagctt gttcaagaat tgccaattca tctgaatcag
    24901 tggataattt atacaaaact tttggataaa tcgactggag taaatcttgt ccttgcttat
    24961 aggtatttgc ccaataaaaa taagaacgca ttcctgctac acctttgatg gcgttagctt
    25021 ttgcagtttc atccaacaaa cggataatct ctgccatctt taagagaagt tcttgttttt
    25081 ggatcatagt tttcgccatc gcacgttctg ccatgacttc taaggcagga agatccattt
    25141 tttccgcatg ttgcatccga tctcttaaag attcatttaa tgggcgattt ccttggtaat
    25201 ttcgatttgt agtgatcaca atgacgcaat ctggatggcg acgaatgatt cctgtcggta
    25261 aattcaacat cccatttggt tcgagcgctg agttaagtgc cacaagaaca gaggcatctc
    25321 ggatgacagt cggctcttga atctctaata aatagccttt ttcaatcgct cgaacgattt
    25381 cagaagggta atagctgtaa tggattgcat ctggataatc agcttcgatc ctttgaacta
    25441 gctctgctaa tttttcttta gcagaaaaag gatcaatgcc gtaatggttc tggatcagtg
    25501 tcagtacagc ttcaagtgta tctgtttggt aaatagcttc tagtaattct tggtcttgtt
    25561 ccttattaga atctactaca ggtaataagg ctccgaaaac atctgatttg tccatatctg
    25621 caaaacaggt cacttttgta tagggaagtt gcagatcagc agatagtgct ttggctaatt
    25681 gtgtcttccc cgacccggca tctccttcca aaagtacgtt actgattttc atttcaggat
    25741 cttgccaatt ccctttgatt tcggaggcga tccgccattc ttctgcactt gtccgatggc
    25801 ttttcggttt cttccagatc atttgctttt ctagccgtga aaaggttcgg ttttctgtta
    25861 gtgagaaatt ctccatgctt ctcttcccct tttgatcatc tctgtctatt aatatcctag
    25921 ctcatctaaa agacgtttta aaatacggaa tcgttcccca atcagttctc cgtctttttg
    25981 tagtgtatcg taatacaaaa tgatgtcttc atcgatcttt ggatttttgg tatctacttc
    26041 taccgtcatt cggttccctt gtaatccaat gatgtctacg actggatttt ctggatcata
    26101 aaatttttca taacgataag catcttggaa gtataaactt ccttgcgcag ctaagatcgc
    26161 taaatctaaa atacgtgtga ttggtaattc ttccgactgt cgtgaccatt tttctccagt
    26221 atgtcgccat accttgcctg agatatcgac ttttccacga tcattccact gagcaaggcc
    26281 cagtgaaagc ccttttgcat ccatatttaa tgcatttctt ccatcaactt tatcatagtc
    26341 ttctacaacg attactggtt tatgttttaa atttgttggt atttccatta ttttaactcc
    26401 tcatcctttt atttttagta aataagtaaa ttagtagttt actcatttac taaactatat
    26461 gcgacattat agaggattga agtgaggatt gcaaagaaaa agagcttcat tgatagaaat
    26521 aaaaacacca tctcgttctt ttacacgctc attagtaaca tgtagaaagt ccaagatgat
    26581 gttggaat
//
LOCUS       DACEUZ010000024        26167 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.024, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000024 DACEUZ010000000
VERSION     DACEUZ010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26167
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.024"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(130..825)
                     /locus_tag="IXG44_000884"
     CDS             complement(130..825)
                     /locus_tag="IXG44_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HAQ1657619.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLTPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(915..2015)
                     /gene="ychF"
                     /locus_tag="IXG44_000885"
     CDS             complement(915..2015)
                     /gene="ychF"
                     /locus_tag="IXG44_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HAQ1657620.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(2024..2215)
                     /locus_tag="IXG44_000886"
     CDS             complement(2024..2215)
                     /locus_tag="IXG44_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HAQ1657621.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(2236..3126)
                     /locus_tag="IXG44_000887"
     CDS             complement(2236..3126)
                     /locus_tag="IXG44_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HAQ1657622.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(3113..3880)
                     /locus_tag="IXG44_000888"
     CDS             complement(3113..3880)
                     /locus_tag="IXG44_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ1657623.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(3892..4605)
                     /gene="rsmG"
                     /locus_tag="IXG44_000889"
     CDS             complement(3892..4605)
                     /gene="rsmG"
                     /locus_tag="IXG44_000889"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HAQ1657624.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(4837..6738)
                     /gene="mnmG"
                     /locus_tag="IXG44_000890"
     CDS             complement(4837..6738)
                     /gene="mnmG"
                     /locus_tag="IXG44_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HAQ1657625.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEVQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(6755..8152)
                     /gene="mnmE"
                     /locus_tag="IXG44_000891"
     CDS             complement(6755..8152)
                     /gene="mnmE"
                     /locus_tag="IXG44_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HAQ1657626.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            8496..8834
                     /locus_tag="IXG44_000892"
     CDS             8496..8834
                     /locus_tag="IXG44_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1657627.1"
                     /translation="MSPFRSAALEEESDPRINDVVLSTGNINQKYIVRDIVFVVECLE
                     ADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQKKRDGKYFLELFAYGT
                     VIQFIQTTIG"
     gene            complement(9002..9760)
                     /locus_tag="IXG44_000893"
     CDS             complement(9002..9760)
                     /locus_tag="IXG44_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAQ1657628.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(9778..10596)
                     /locus_tag="IXG44_000894"
     CDS             complement(9778..10596)
                     /locus_tag="IXG44_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAQ1657629.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(10593..10949)
                     /gene="rnpA"
                     /locus_tag="IXG44_000895"
     CDS             complement(10593..10949)
                     /gene="rnpA"
                     /locus_tag="IXG44_000895"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HAQ1657630.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(11067..11201)
                     /gene="rpmH"
                     /locus_tag="IXG44_000896"
     CDS             complement(11067..11201)
                     /gene="rpmH"
                     /locus_tag="IXG44_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HAQ1657631.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            11725..13059
                     /gene="dnaA"
                     /locus_tag="IXG44_000897"
     CDS             11725..13059
                     /gene="dnaA"
                     /locus_tag="IXG44_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HAQ1657632.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            13257..14387
                     /gene="dnaN"
                     /locus_tag="IXG44_000898"
     CDS             13257..14387
                     /gene="dnaN"
                     /locus_tag="IXG44_000898"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HAQ1657633.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            14616..14858
                     /gene="yaaA"
                     /locus_tag="IXG44_000899"
     CDS             14616..14858
                     /gene="yaaA"
                     /locus_tag="IXG44_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HAQ1657634.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            14845..15969
                     /gene="recF"
                     /locus_tag="IXG44_000900"
     CDS             14845..15969
                     /gene="recF"
                     /locus_tag="IXG44_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HAQ1657635.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            15966..17912
                     /gene="gyrB"
                     /locus_tag="IXG44_000901"
     CDS             15966..17912
                     /gene="gyrB"
                     /locus_tag="IXG44_000901"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HAQ1657636.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            17932..20403
                     /gene="gyrA"
                     /locus_tag="IXG44_000902"
     CDS             17932..20403
                     /gene="gyrA"
                     /locus_tag="IXG44_000902"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HAQ1657637.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDRAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKENQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            20639..20938
                     /locus_tag="IXG44_000903"
     CDS             20639..20938
                     /locus_tag="IXG44_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HAQ1657638.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            20988..21500
                     /gene="ssb"
                     /locus_tag="IXG44_000904"
     CDS             20988..21500
                     /gene="ssb"
                     /locus_tag="IXG44_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ1657639.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            21526..21762
                     /gene="rpsR"
                     /locus_tag="IXG44_000905"
     CDS             21526..21762
                     /gene="rpsR"
                     /locus_tag="IXG44_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HAQ1657640.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            21905..23878
                     /locus_tag="IXG44_000906"
     CDS             21905..23878
                     /locus_tag="IXG44_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HAQ1657641.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            23884..24336
                     /gene="rplI"
                     /locus_tag="IXG44_000907"
     CDS             23884..24336
                     /gene="rplI"
                     /locus_tag="IXG44_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HAQ1657642.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            24610..25977
                     /gene="dnaB"
                     /locus_tag="IXG44_000908"
     CDS             24610..25977
                     /gene="dnaB"
                     /locus_tag="IXG44_000908"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HAQ1657643.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNIIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
BASE COUNT         7941 a         5157 c         5188 g         7881 t
ORIGIN      
        1 tttatcgtaa aaagggactg tgacaagact tttgtcacag tcccttacta ttatttaact
       61 atggataaac aaaatgaggt tctttactac ccctagatat ctagcaaaat agctgatctt
      121 tttgctagtt tatcttcttg ggacggataa acttccaata atcccgtatt ttttcttcaa
      181 gtagtaacgg attgctaaag cagttgcccc gataatgata actactactg gatctaatac
      241 tggattgata actggtgtca atagtgttgt cgcagaaaac agtacgaccc aaagaagtgt
      301 cgttccacct aggataagca atgtcttacc cattcctggt ttcttgcttc gatctgctcc
      361 gggatattca tattgataga tgtatttgta catcatataa aagacccagc caccgattgc
      421 agaacctaga acgagtgtag aaaagccata agctggcgtt gttccacgtg aaaacaatcc
      481 ggtaatcgca atcatgatac ttaatagacc aagcaacagc aacgtattat ctaaccacat
      541 caataaaggg gtggatgaat tttttacttc tgctggctta ttgatgattg cttccgttct
      601 ttcagagact gtcccaaaca attgacgggc tgttcttccg cctttttgtt ctgcaactaa
      661 agtaggcaga atgtcatgaa gggcttttac tttttcttct tcagacaaat tcgctgcttc
      721 taaagatttt ttcaaatcaa aaatgtactg ttgatttctt ttggttaatt ttgtttctaa
      781 actattgttt tcttttacga ttgctcgtaa ttgttctggt tccattatct aatagtcctc
      841 cttagccgtt ccttttctca acggtcgtca caaaagacat tctaccatat tctacagcta
      901 gtttgatagc ccttttacac attaaagcgg aataacatta cgtcgccgtc ttgtacgaca
      961 taatcttttc cttctaatcg aacacgtcca gcttcttttg ctgcatgcat gtttccgtaa
     1021 tgatccaagt cttcaaaaga gacagtttct gcacggataa atccgcgttc gaaatctgaa
     1081 tggatgattc ctgctgcttg tggtgctttg attccttttt tgaatgtcca agcacgtact
     1141 tcttgttccc cagcagtaaa gtaagtagct aagcctaaca aatcataagc agcacggatc
     1201 aactgatcca aacctgattc ttcgattcct aaagcttcta aaaattccgc tttgtcttct
     1261 tcatctaatt ctgcgatttc ttcttctgca cgagcactga ccacgatcac ttctgcattt
     1321 tcttctgcag caaagttgcg cacttcttgt acataatgat tcgcgtctgc atctgctacg
     1381 tcatcttctg agacattggc tacataaaga attggttttg tcgtcaataa aaatagtgat
     1441 ttaacgattt tttgttcttc ttcagtaaat tcgatcgttc gggcagattt tccttcttcc
     1501 agtacaggtt tgattttatc cagtacagct agttctgcca ctgcatcttt atctttggtt
     1561 ttagcaactt ttgctacacg agtatagcgt ttgttgatag attctaagtc tgctaacact
     1621 agttccaagt tgatcgtatc aatatcttct agtggatcaa cacgtccttc tacgtgagta
     1681 atattatcat catcaaaaca gcgaacaacg tgacaaatag cgtctacttg gcggatatgg
     1741 cttaagaatt gatttcccaa accttctcct ttacttgcgc cttttacgat tccagcaata
     1801 tctgtaaatt caaaagtcgt cggaacagtt tttttcggat gtacaagttc tgttaagcgt
     1861 tgcaaacgcc agtcaggtac ttctaccatc cctacgttgg ggtcgatcgt cgcaaaggga
     1921 tagtttgctg cttctgctcc tgcttttgtg atcgcattaa acaaagttga ttttcctacg
     1981 ttgggcaacc caacaattcc agcagtcaat gccataaatt tattcactct ttctctgcat
     2041 ttttttcaat tacttttttt aatttctttt caaaatctcg acgggtcatc atgacgatat
     2101 gtccgcaatt catacatctg attttgatat ctgcgcccat tcgaatgact tcccagcgat
     2161 tcgtctggca agcgtgaggt tttttcattt caacgatatc gtgcaagtcg tacataaaaa
     2221 tagcctcctt tttgtttatt cttcatcaaa ttgaatattc aagatatcaa gaattcgagt
     2281 caaatctttt tgagaaagat attcaatctc gatcttacct ttcccatttt tttcttggat
     2341 agctacactt gtaccaaatt tatccatcaa acgatcttcg ctttctcgga tataatatgg
     2401 tttttccttt gctagccttg ggattttttt cttttctttt ccatcatttt gatttaactc
     2461 agaaacaagc tgttccagtt ggcggacagt taaattttca gttactgcac gattggctaa
     2521 tttcaagatc aatgttttat tttttaaacc taacagcgta cgggcttgtc ccatagataa
     2581 acgctgatct tgtaccattt ccttgacaag atccggtaaa gaaagcaagc gcaaataatt
     2641 ggcgatataa ggtctgctct ttcctaaacg actagctact tctgcttgtg ttagtttcag
     2701 gtttttcatc agcatctcat atgcttctgc ttcttctaac ggattcagat cttctcgctg
     2761 caagttttcc agcactgcta cttgcatcat cgcttcttcg tcaaagtctc taacgatagc
     2821 agggatcgtt tctttttcag ctagtttgga cgctcgaaaa cgtcgctccc ctgcaataat
     2881 ctcatatcct tttactgcgg aacggcgtac gatgatgggt tgaaagacac ctgattgttc
     2941 aatagaatta gctagttcct gcaaagatac ttcttcaaat gttttacggg gctgatacgg
     3001 atttggtcgc agctcattca atggtagctg gacaacggta tcatctttta catccaccgt
     3061 ctctaagttt tctaaatctt gaaacagtgc atcgattcct cggcctaatc ctttactttt
     3121 cttcacgact taccacttcc tttgccaatg cttggtagac ttcagctcct cgagaacgtg
     3181 gatcgtagtc gatgatcggt tttccatgac taggtgcttc tgataaacga atatttcttg
     3241 gaatgatcgt ttcgtaaact ttttcgcgga agtatttacg tacttcttct acgacttcat
     3301 ttcccagatt tgttcgtgca tcatacattg tcagtaagac tccctcgatc tctaattctg
     3361 gattaaagtg tttttggact aaacgaaccg tattcagtaa ttggcttaat ccttccaatg
     3421 cgtaatactc acactgaaca ggtatcaaaa tcgaatcact tgctgtaaat gaattgattg
     3481 tcagatggcc aagagaaggc gggcaatcga tcaaaatata atcatactga ctgcttactt
     3541 ctgctaaaga tccttttaat cttgattctc ttgccatcat tgacgtcaat tcgatctctg
     3601 ctcctgctaa ttggagagtc gcaggcacaa tactcaaatt ttcgtgttca gtgatcaaag
     3661 ttgcctcatc aattggcaat tcattgacta gaacatcata gatgtctctt gtcacatcag
     3721 gttttctgat cccgacacca ctggtagcat ttccttgtgc atccatatcc actagcaata
     3781 cttttttgcc aaggctagca agacaagcac ctagattcac cgtcgtcgtt gttttgccga
     3841 cgccaccttt ttggttcgca acagatatta tttgtgccat ctcttctcct cctatttgat
     3901 tggctgctta ttaggcagtc cgggctttct tgggtatttc tttggggttg gtttcttttt
     3961 ttgaataacg atgatatgcc gttgatcaga gacattcggc aaggtaaccg cctcttcttt
     4021 gaggaattta ccacctaata aagcaatcgc ttgttttgct tctgtcaatt cctcttcact
     4081 ttttgctgct ttcaatgcat aaaaatagcc ttcttgttta gccaagggca aacacaattc
     4141 cgctaacaca ttcaaacgag caaccgcacg agcagtgaca aggtcaaatg atgcacgaaa
     4201 gtgaggattt tgcccaaatg tttctgcccg gtcatgatat aaggaagcat cgattcctaa
     4261 taatcgggtt aactcagata agaaggttat tcgtttattc agagaatcaa cgatcgttat
     4321 ctgcagttct ggaaaggcaa tttttaaagg tatgctagga aaccctgccc ccgaaccaac
     4381 atcgcataaa gataaaggtt gcgtaaagtc tacttcaaat gctaatgtaa gtgaatcgta
     4441 aaaatgcttc agatatacgt ctttttcatc cgtaatagct gtaaggttca tcttttcgtt
     4501 ccattcgact aatgtccgat aataaatagc gaattgattc atttgatgat cggtaagctc
     4561 tatccctttt tctgccaagg attggcgaaa ttcttctggt atcataaata ttcctttcat
     4621 tgtgaaacag caattgtttc acagattctc tccctacttt aacgcaaaat ctctagacat
     4681 gcaatccttc catatggatt cttaaaaatt tgaaaagtaa gaaaagccga tagacttttt
     4741 ctttctagta tgactcataa aatatacaca gaagagagaa ctagctttga gcaatttctg
     4801 caaaaactag ttctctcttc tatttattat tacgtattac gctggttcac tgactttcgc
     4861 aatcttccct tgttcgatat agaccattag gatacttagg tctgcagggt ttacgccact
     4921 gattcggcta gcttgggcaa ttgtttctgg ttggattttc tgcaattttt gttttgcttc
     4981 tgttgctaat ccgttgattg ctgcgtagtc gatgttttct ggaatctttt tcgcttccat
     5041 gcgttttaat ttttctactt tttccatggc ttttttgatg tagccttcat atttgatttg
     5101 gacttctact tgttcgattt cttttcgtgt caattcttca ttttctggaa taaaccgtag
     5161 aagttctgca taagtaatct caggacgtct taaaaagtcg cttgctagta caccatcttt
     5221 taatggtgcc gcattaatcg ttgctaaaaa gtcttggact tcttctgttg gtttgattcg
     5281 tacattcatc aaacgtttga tttcttgttc aactgcttct tttttcttca aataattctt
     5341 gtattgatct tcttttacta agcctacacg gtgacccatt tctgttaatc gcaaatctgc
     5401 attatcatga cgtaaaagca gacggtattc agcacgtgaa gtcagcaaac gataaggttc
     5461 gttcgttcct tttgttacca aatcatcgat catgacaccg atgtaaccgt cacttcgttt
     5521 gatcactaaa ggttcttttc cctgaacttt caaagctgca ttgataccgg caatcagccc
     5581 ttgtccagcc gcttcttcat aaccgcttgt accatttgtt tgtccagctg tataaagatt
     5641 ttcaacgact ttagtttcta atgtcggacg gagctggtgt ggctctacta cgtcatattc
     5701 gatagcatat cctgtacgca tcatttctac tttttctaat ccagcaatag aatgaagcat
     5761 gttgacttga acatcctctg gtaaagaagt agataaacct tgaacataga cttcttccgt
     5821 atttaagcct tctggttcca aaaataattg atgacgtggt ttgtctgcaa aacgcacgat
     5881 cttatcttcg atggatggac aatagcgagc gcctacccct tcaacgatcc ctgtaaacat
     5941 tggggcacga tgtaagttgt ctcgaataat ttggtgagtc gtctcacctg tataggtcag
     6001 ccagcaagat tcctgatcta gtttataggc actatctggt gtgctgaagc taaaatgatt
     6061 cggctctttg tcaccaggct gctcttctgt aacagaatag tcgatcgtac ttgattttac
     6121 tcgaggcggt gttcctgttt tgaaccgttc gatagcaaag ccaagttctt ttagatgatt
     6181 cgctagtcca atagaaggtt gagagttatt cggacctgat gaatatttca attcaccgat
     6241 aatgatttct ccgcgaagtg ctgttccggc tgtaataaca actgctttgg cccggtagct
     6301 cgctcctgtg gacgtcacta ctccatgaca aacaccatct tcgacgatca gttcttcgac
     6361 gattccttgt cttagcgtca aatttggttc tttttcgatc gtatgtttca tttctgttga
     6421 ataagcatgt ttgtctgctt gtgctcgcaa tgctcggaca gctgggcctt ttcctgtgtt
     6481 caacatacgc atttggatat aggttttatc gatattctta cccatttctc cgcctaacgc
     6541 atcgatttct cgaacgacaa cgcctttagc tggtccgcct acagaaggat tacatggcat
     6601 gaatgctacc atgtccaaat ttattgtcag taataatgtt tttgatccca tacgtgcagc
     6661 agcaagtgca gcttctgaac cggcgtgtcc tgcgccaaca acgatcacgt catacgcatc
     6721 tgcttcataa tgttccaatc gtttgttcct ccttttattt tcctaaacaa aattgactga
     6781 ataattgagt gattagctca tcttgtacac tgtcgccgac aacttctcct agatagtccc
     6841 agcaacgcgt catatctatt tgaacgagat ccacaggcat tcctgaatca atacctgcaa
     6901 tgacttcttc aagtgagagg gaagcttttt ctaacaacgc gatatggcgg gtattagaaa
     6961 cataagtagc gtctctttct cctgtttctc cgctgaagaa taaatcagaa atagctgatt
     7021 ccaaacgatc tagtccgtcg tttttagcta cggaaattga aaaaactggt tcattttcga
     7081 tcaatttctt caatttttct tgttccagct gtgctggcaa atctgtttta tttagaagaa
     7141 tgatccgttt caatccactt gtcgcttcca ataattgttc atcttctgct gtcagtggtt
     7201 cgctttggtt tagaacaagt aagatcaagt ctgcttctgc taatgctttt cggcttcttt
     7261 cgaccccgat ccgttccacc acatcttctg tttcccgaat tccagctgta tcgattagct
     7321 tgagcggtac cccccgaaca ttgacatact cttcgattac atctcgagtc gttcctgcaa
     7381 tatccgtaac gattgctttt tcttctctta acaaatggtt taacaaactt gatttgccga
     7441 catttggccg gccgatgatt gccgtactta acccttcccg cagaaccttc ccttgcttgg
     7501 atgtagcaag tagcgcttgg attctttgtt ggatcatctg cgctttttca agcaataact
     7561 tcgtagtcaa ttcttcgaca tcatcatatt ctggataatc aatatttact tctacttgtg
     7621 ctaatgtctc taaaatctct tgtctcaatg agcggatcaa agcagacaga ttgccgtcta
     7681 actgattaag agctagtccc attgctttgt ctgtttttgc ccgaatcaaa tccatcacag
     7741 cttctgcctg tgaaaggtca acacgaccat tcaagaatgc tcgttttgtg aattcgcctg
     7801 gctcagctaa tcttgcacct tctcttaaca atagctgcaa aatttgattg actacaacga
     7861 tcccaccgtg acaattgatt tctactacat cttcccgcgt aaatgtcttt ggtgctctca
     7921 tcacggagac catgacttca tcgacaagct gattgctctt gggatcaacg atatgtccat
     7981 aatggatcgt atgacttggt acttctgata atcgtttgtt tccagattga taaactttat
     8041 ctgctagttg cacagcttga tcgccactta aacgaacgat actgattgca ccttctccag
     8101 gtggggtaga aattgccgca atcgtatcaa attctaaagt aatttcagcc attttttcac
     8161 ttcctttttc attttgcgca caaaaaaagt gcccactcca ccctttatgc acaataaacg
     8221 gtgaacttcg cactttgact gcttgttctt gattaaattc tggtcataga ttatcacact
     8281 tgctataagg aatcaagtgt ttaaaacaat tagatgaagt tgagcctgtt ctgcttccat
     8341 taccttatat ttaattgtcc ttggatgtct ctttgcgttc tttggtttat tcgaagagaa
     8401 gatactgtat aaacaaggct ttatctttct taaaaaaaac ggtacactaa atggtagcgc
     8461 tatctaaact atacactttt gaaaggagaa aatatgtgag cccgttcaga tctgctgcat
     8521 tggaagaaga aagtgaccct cggatcaacg atgtcgtttt atctacaggg aatatcaacc
     8581 agaaatatat cgtccgcgat atcgtttttg ttgtggaatg cttagaagct gatttgtttg
     8641 acccatcttt tcccaaagat gaccttttct taacaacgaa gaggaaatta aagacgaagg
     8701 tacttgaata tggtgcgaat gcagtaatca actgtcattt tgagtatcag caaaaaaaac
     8761 gcgacggaaa gtattttctg gaactttttg cttatggaac agtgatccaa tttatccaaa
     8821 caacgattgg ttaaatgaaa cagacaaaaa aagcggatca aaactgaaga cagtttcgat
     8881 ccgctttttt ctatcaaagt tagtgactac ttcatcgtat actctgataa gaatgatttc
     8941 tagtaaaata tacttggttt gaaaaacttt gtcaacgtga tttattcaaa aagtaatgga
     9001 atcaataata ttttttagca ggttctacta ctaaataacg ataaggttcc tcaccttccg
     9061 aatgtgtttt tacatgttca tctttgctta atgcagcatg gatttgttta cgttcaaatg
     9121 caggcattgg ttctaagaaa acaggctgcc ctgtccgttt tactttttca gccgtgcgat
     9181 cagctaaacg ctgaatgatc tcttggcgct tttcacggta attgcctaca ttgactacta
     9241 cagacagctt atttcttgct aaacgatgga tgaagacttg tgccagatat tgcaatgcat
     9301 tcagtgtttt tccatgtttc ccaatcagta tgccttgctt ttgtgtttct aaatggaaaa
     9361 caaccatatt atttgattct ctttccattt tgacaagagc tggagcatca agctgttttg
     9421 aaatttcagt aagataaact gccaattccg ttaaagctgc ttcatcacct agctcactta
     9481 cttcttctgt tttttcttca agaatcgttt cgttgactac ctcgatctca gctgttggtt
     9541 cttcttccaa aatttcttct acagtctgtt ctactgtttc agaaactttt tcgctgatac
     9601 ttggctggat cgaaagccgt gcattctttc gacccattcc taggaaacct ttttttcctt
     9661 cttccagtat ttcgatttcg acttggtctt tggccaaacc caaatcagcc agacctagtt
     9721 ggacagcttc gtctattgtt gcagcttcat aaatcggcat tagcattacc tcccctatta
     9781 tttccgtttt ttcttaggat tcattgcttt cttaagagca cgttctcttt cgcggattct
     9841 ccgtgcttct tcttcacgct cttttcgaat tttgaaaggg ttgttgatca gcagcgtttg
     9901 tacaacttgg aaggcattgg aaactaccca gtataatgaa agaccgctgg ctaaattgac
     9961 acccatcact aaaatcatta caggcatcac gaaattcatg atcttcattg atgcattcga
    10021 ttcaatttga ctcatgctag ataaatacgt acttgcaaaa gtaaagattg ctgctaaaat
    10081 cggcaaaata aatgttggat ccgcgttccc taagttcaac cataagaaat gcccttgttt
    10141 caactctggt acacgtgaaa tagattgcca taacgccatc aagattggca tttgtactaa
    10201 taaaggaaga caaccagcat atggattgac gccattttct gcatataaac gctgctgctc
    10261 ttctcttaac ttactttgtg tttccggatc tcttgaacta tattgttgct gcagtgcttt
    10321 taatttcggt tgtaattctt gcgttttacg catgctcttt gtttggaaat gcatcaacgg
    10381 caataggatc acgcggatga tcagtgtaaa taaaatgatc cctatccctg cattaccaaa
    10441 tgacaacgcc ttgatcgcct cagcaaagta ataaacaata taacgatccc aaattcctgt
    10501 actctgtgcg ctgacttccc cagttccgca accggataag acgatgacca aactaacgat
    10561 accggccatc aaaagtaaac gtttgtattt cttcacttac tcttttcctc tctgtcgatt
    10621 atttttgcaa gttttaagac gtggactaaa ttggagcgaa cctcatcata agtcaacccc
    10681 ttcacacttg gtctagcaat cacaataaaa tccaactccg gatcaatttc ttcttttata
    10741 gaataaactg ctgctcggat ttttcgtttg acttcattgc ggtttaccgc gttgccgacc
    10801 ttttttccta cagataaacc aactcgaaaa tgaggctgtc ccggtttttc caaacggtaa
    10861 acgacgaatt tgcggttagc aaaagaatct cccgtatgga acacggcttg aaactctcgt
    10921 tcttttttta ctcgatacgc ttttctcatt agttttcttt ccttttctat ctctcactta
    10981 cgctcataat cataccataa ttacgattgt cctttcatca tttccctaaa aaagagtata
    11041 aaaaaaacca ctgaaacccc agtggcctaa gctgaaagaa cttttcttcc tttgcgacga
    11101 cggctagcta acacacgacg tccattttta gtactcatac gtttacggaa tccgtgaact
    11161 ttttgacgtt tacgtttatt tggttggtat gttcttttca tatattccac ctccaaaatg
    11221 ttcttatcta ttttataaac acagacatac tttgatagta taacgacaaa gtagtgattt
    11281 tgtcaatata aaatagaaga tcgattgtta aaacttatcc acaagattat tttttttcta
    11341 tgggaatcct atgtggaaaa agttatgcac agggattatc cacagataat aaaaggctgg
    11401 ttttacacag aaaacacacg tgtaaaaaaa gtaatccaca atcaggcgat ttcctgtgta
    11461 ttccgtctcc taactattgg tatatctgta ttttttatcc acagacgcaa gaggaaatct
    11521 attggttttt acgagttttc cacctgtgca ttagtggtgt ggaaaacttt ttttgacaac
    11581 tgtgtttttc tcttttttct ttttgtcgat tgtgtggata attgtaatta aaaaattttt
    11641 ttcttcggtt tctgtggaaa actattgaaa aaaatgctaa attagtacta gttattcact
    11701 ttttaatgaa ggaggaggcc ttatatggta tccctcgatg ctttatggaa tgaattaaaa
    11761 gcaacatacc aaaaggattt atcgcctgct agctataata catggatcga aacagcgaac
    11821 cctcgaaccc ttgatcaaaa ccagctcgtt gttgaggttc ctagcaagat tcataaagaa
    11881 tattgggaaa aaaacctggc gacaaaaatc gttgaagtcg gttatatgtt atcaggaaat
    11941 gagattatcc ctcgctttat tactggcgaa gaagcagagc aagaagaagt tatagaagaa
    12001 aaaaatccaa aagttgtggc acctagtccg ctaaaaaaag ccatgctgaa ccctaaatat
    12061 acctttgata cgtttgtcat tggtaaagga aaccagatgg ctcatgcagc agcgctcgtt
    12121 gtagcagaag atccaggttc tatttataac ccgctcttct tttatggagg cgtgggactt
    12181 ggtaagactc acttgatgca tgcaattggt catcaaatgc tgcaaagtca gcctaatgcc
    12241 aaagtaaaat atgtaagcag tgaaactttt gctaatgatt tcatcaattc gatccaaaac
    12301 aaaacagcag aagagttccg tcaagaatat cgaaatgttg atttactgct agtggatgac
    12361 attcaatttt ttgcagaaaa agaagctacg caagaagagt ttttccatac tttcaatgcc
    12421 ttgtacaatg aagggaaaca aatcgtacta accagtgatc ggttgccaaa cgagattccg
    12481 aaactgcaag aacgccttgt ttctcgcttt gcatgggggc tgtctgttga tattacaccg
    12541 ccggatcttg aaacaagaac agcgatttta cgtaaaaaag cggcggctga acgtctagag
    12601 atcccagatg acacactaag ctacatcgca ggacaaattg attctaacat tcgtgaatta
    12661 gaaggtgcgc ttgttcgagt gcaagctttt gctgcgatga atagtgaaga tatttcgact
    12721 agtctagctg cagacgcact gaaaacgtta aaatcaggaa aaggacatcc tcaattgtca
    12781 atcctgcaga tccaagaaga agtggcaaag tattatcaca tccaattgaa agatctcaaa
    12841 ggaaagaaac gcgtcaaatc tattgttgtt cctagacaaa tcgctatgta tttggcacgt
    12901 gagctcacag ataattcttt accgaaaatc ggagctgaat tcggtggcaa ggatcataca
    12961 acggttatcc atgctcatga aaaaatccag cagctgatgg attctagtgt cagtatgcaa
    13021 aatgaagtat cagaaatcaa aaacttttta ctaaattaat ttgtcgattc tatcaactga
    13081 aaatagcatg tggataaaag aaaaaaacgt tcaaaagtta tacacacttt ttccacagct
    13141 gttttttcgt ttctctctta cgttttttga gttttccaca gaatcaacat gccctattac
    13201 ttttattatt ctttttaact aataataaat aaacaaacat tcaaaggaga tacatcatga
    13261 aagttacttt aaaccgagct agctttatgc aggaattgca aactgttcaa cgagctattt
    13321 caagcaaaac cacgatccct attttgacag gtgtaaaaat cacactgaca caagaaggtt
    13381 tgactttgac ggggagcaac gctgatatat caattgaaac ttttttgtct gttgaaaacg
    13441 aaaaagcaaa tatgcaaatc gaatctactg gttccattgt tttacaagca cgtttcttta
    13501 gcgaaatcat tcggagactt cctgaagaaa catttacttt agaagtttta gaaaataaac
    13561 aagtagcgat cacttctgga aaagcgaatt ttatcgtaaa tggattagat gcagataact
    13621 atcctcatct tcctgttgtc gaaagccata accagatgaa attacctgta cacgtattga
    13681 ctaaactaat caacgaaaca gtttttgctg tctcacaaca tgagagtcgt ccaatcttga
    13741 caggtgtgca tttcatttta tctgataatt ctttattagc tgtagctact gattctcacc
    13801 gtctaagtca acgcgtgatt ccagtagaac aagcggctga tcattttgat attgttattc
    13861 ctggaaaaag tttgatcgaa ttatctcgct cattaacaaa tgaagaagaa atcgtcgaaa
    13921 tcagcattat ggaaaaccaa gtgttgttca aaacagaaac gatgtatttc tattcccgtt
    13981 tgctagaagg aaactatcca gataccaatc gtttgattcc ttcaagtttt aatacagagg
    14041 ttgaattttc tgttcctagc tttttagcgg cgatcgaacg ggcttcttta ctttctcatg
    14101 aaggccgtaa caacatcgtt cgtttatcta ttcgtccaga tgcggttgtt ttatacggaa
    14161 attcgccaga aatcgggaaa gtagaagaaa gcttgagtta tacagcaagc agtggcgatc
    14221 cattagatat ttctttcaat ccggattata tgaaagcagc gttgcgtgca tttggagata
    14281 tgagcatcaa agtgaaattt atctctgcta ttcgtccatt tacattggaa ccaacggaag
    14341 atggcgttca atttattcag ctgattactc ctgtacgtac aaattaacaa tgattttcaa
    14401 tgttcttgaa aatttaattg aaaagaaata gaaaaaataa aattttaaac agaaggccct
    14461 aaaatctctt ttaaggcttt ctgtttattt ttgtttaaat cacctttttt agattaaatc
    14521 gtcttatatg gaaaaatata tggataaatt tgttttattg cgaaaaaaaa ggtataatag
    14581 agtatacttt taataatgaa aatgagggat gggcattgaa acaacaagtc attctaaata
    14641 cggaattcat gacgttgggc caaatgttga aagaagtcac agttatcggt agtggcggac
    14701 aagcaaaatg gtatttggca gaaaacactg tgttagtcga tggcgaacca gaaaatcgtc
    14761 gtggacgcaa actttatccc ggcatgatgg ttgaagttcc agaagtagga acttttttta
    14821 tggtaaaaaa aggaaacgac gataatgagg ctgaataagc tgtatttaaa aaattatcga
    14881 aattacgaag agctgaacat cgaattttcc aagaacctcg tcatcttttt aggagaaaat
    14941 gcgcaaggaa aaacgaatct tttggaaagt atctacgtat tagctatgac gcgaagtcat
    15001 cggacaagta gtgagcagga attgattggc tggactgacg atcaggcgat gattcaagga
    15061 gagatcacaa aaggttcatc cactcttcca ttagaaatct tattatctaa aaaaggccga
    15121 aaaaccaaga tcaaccatat cgaacagaaa aagttaagta cttacgtggg acaaatgaat
    15181 gtcattttat ttgcaccaga agatctttct ttagtaaaag gcagcccgca gatcaggcgg
    15241 aagtttttag atatggaatt aggccagatc aatccaatct atctttatga tcttgtccaa
    15301 taccaagcaa ttttgaaaca aagaaaccag tacttgaagc aattgaatga aaaaaaacaa
    15361 actgatttgc tttatttgga tattttgtcc gaacaactag cagcgtttgg cagcaaagta
    15421 ttgaaagcaa gagcacagtt cgtcaagcga ttggagtttt gggctaattc tttgcatcag
    15481 caaatcaccc atcaaaaaga gcagctggaa atcgagtatc tcaccgcagt cgattcttta
    15541 gaaacacata cgcaagagca aatccaagaa caatttctcg cacttttgaa ccaaaataaa
    15601 aagaaagacc tttttcgggg aacaacgaca gttggtcctc atcgtgacga tctttccttt
    15661 tttattaatc agaaaaatgt gcagacttat ggatcgcaag gacagcaaag aacaactgca
    15721 ctaagtgtca aattagcaga aatcgatttg attaaagaag agacagggga atatccaatc
    15781 cttttgttag atgatgtgat gagcgagctg gatgataatc gtcaactcca tctgctagaa
    15841 acaatcgaag gaaaagttca aacgttctta acaacaacga cacttgacca tgtaaaaaat
    15901 aaaatgactg ttgaacctga gattttttat gttcaacaag ggaaactaaa aggagaagaa
    15961 actgaatgac agaagaaaga agtttagtag aacgcgctaa agagtatgat gccagtcaga
    16021 ttcaagtact tgaaggactt gaagccgttc gtaaacgacc tggcatgtat atcggttcga
    16081 ctagttctga gggtctgcat caccttgttt gggaaattgt cgataattcg atcgatgaag
    16141 tattagcagg ttttgcaacg aaaatccatg tgattattga aaaagacaac agtatcaccg
    16201 ttattgacga cggtcggggg atacctgtag atatccaggc aaaaactgga cgtcctgcag
    16261 ttgaaacggt ctttactgtt ttacatgctg gaggaaaatt cggcggtggt gggtacaaag
    16321 tttctggtgg acttcacgga gtaggatctt ctgttgtaaa cgcgctttct acacagcttg
    16381 atgtcaaagt atacaaagaa ggaaatgtgt actatcaaga atatcgtcgt ggagctgtag
    16441 tggacgactt aaagattatc gaacaaacgg atcgccatgg aacaacggtt cactttacgc
    16501 cagaccctga aatttttaca gagacaactg agtttgattt ttcaaaattg gctacccgga
    16561 tccgtgaact tgctttcttg aaccgcggga tgcgtatatc gattgaagac aaacgagaag
    16621 aagaaccagt catcaatgaa taccattatg acggcgggat caaaagttat gtcgaatact
    16681 tgaatgcaaa caaaaccgtg atttttccag aacctgtata tttggaaggt gaacagcaag
    16741 atatcgcagt tgaagtctct atgcaatata cagacggcta ccattccaat atcatgagtt
    16801 ttgccaacaa tatccatacg tatgaaggtg gaacacatga atcaggattc aagactgctt
    16861 taactcgtgt gataaatgat tatgcccgaa aacaaaagct gatgaaagaa aatgacgaca
    16921 acttgaccgg tgaagacgtg cgagaaggat tgacagctgt tatttctatc aagcatcctg
    16981 accctcaatt cgaaggacaa acaaaaacaa aattgggcaa ttccgaagta cggacagtca
    17041 ctgatcggtt atttagcgaa catttcatga aattcttgct ggaaaaccca agtgtgggcc
    17101 ggcaaattgt tgaaaaagga ttactagctt ccagagctcg tctagcagcg aaacgtgcgc
    17161 gtgaagtaac gagacgtaaa ggtgctttag agattagtaa tctgccagga aaattagcgg
    17221 actgctctag caacgatcct gaaaaatgcg aattgttcat cgtcgaagga gattcggccg
    17281 gcggatcagc taagcaaggt cgtaatcgag agtttcaagc catcttgcct attcgtggga
    17341 aaatcttgaa tgttgaaaaa gcaagcatgg acaagatctt ggcaaatgaa gagatccggt
    17401 ctttatttac tgctatgggt acagggtttg gagaagattt tgatgtatcg aaagctcgct
    17461 accacaaatt agttatcatg actgatgccg atgtcgacgg cgcgcatatc cgtacattgc
    17521 tgctgactct tttttatcgt tacatgcgtc ctattgttga agctggatac gtctatattg
    17581 ctcagccacc tttatacggg gtaaaacaag ggaagaatat tacgtatgta caaccaggaa
    17641 aaaatgcaga agaagagttg aaacaagtcg ttgcttcact tcctgcctca ccaaaaccaa
    17701 gcgttcagcg ttataaagga cttggagaaa tggatgatca tcaactatgg gaaacaacta
    17761 tggacccaag caatcggatg atggcccgcg tcagtgtgga cgatgctatt gcagcagatc
    17821 agatatttga aatgttgatg ggggatcgag tagaaccccg tcgtgcattc atcgaggaaa
    17881 atgcacacta tgtgaagaac ttagatattt aatcatgaag gaggaaaaag aatgagtgaa
    17941 gaaatcagag aaaacatcca tgatgtcaat ctaaccagcg aaatgaaaga ctccttcatc
    18001 gattatgcga tgagtgttat tgtcgcacgt gcacttcctg atgtcagaga tggtctgaaa
    18061 cccgttcatc gccgtatttt atacggcatg aacgaattag gtgtaacacc ggataaagcc
    18121 cataaaaaat ctgccagaat cgtcggagat gtcatgggta aataccatcc tcatggagac
    18181 cgtgcgatct atgagtcaat ggtacggatg gcacagccat tcagctaccg ttatatgcta
    18241 gtggatggtc atggaaactt tggttctgtt gacggtgacg gtgccgccgc tatgcgttat
    18301 accgaagcac ggatgagcaa aatcgctaca gaaatgctgc gtgatatcaa taagaacact
    18361 gttgatttcc aaagcaacta tgatgataca gaaaaagaac cagttgtact acctgctcgc
    18421 ttcccgaatc ttttagtgaa cggaacaacg gggatcgccg taggtatggc cacgaatatc
    18481 ccacctcata acttgtctga ggtagtagcg gctatcgatt tgctgatgga taatcctgat
    18541 gtgacgacca atgaattgat ggaagtatta cctggaccag attttccaac aggtggtttg
    18601 gttatgggga aatcaggaat ccgaagagcg tatgaaacag gtaagggttc tattaccgta
    18661 cgtgcaaaag ttgagattac tgagatgcca aatggtaaag aacggatcat cgtgacagaa
    18721 ttgccttata tggtcaataa agcaaaattg atcgaacgga tttctgaact gcacagagac
    18781 aagcgtatcg aagggatcac agatttgcgg gacgaatctt cccgtgaagg tatgcggatc
    18841 gtcattgaca tccgtcgtga cgccagcgca tccgttatct tgaataactt atataaattg
    18901 acatccttgc aaaattcatt tggattcaat atgttggcga tcgaaaaagg tgtgccgaaa
    18961 gttttgagct tgaaacaaat ccttgaaaac tatatcgaac atcaacgcga agtcatcaca
    19021 cgacggacgg tctttgaaaa agaaaaagca gaagcaagag cacacatctt agaaggtttg
    19081 cgtattgctt tggatcatat cgatgaaatc attgcgatta tccgcgggtc taaatcagat
    19141 gacgaggcaa aagcaacaat gatcgaacgg ttcgatttat ccgatcgcca gtctcaagct
    19201 attttagata tgcgtttacg ccgattgacc ggtttggaac gggaaaaaat cgaaaatgaa
    19261 taccaagaac tcttaaaact gattgaagat ttaactgaca ttctggcaag accagaacgt
    19321 gttacagaaa tcatcaagaa tgaattagca gacttgaatc aacgatttgg tgataaacga
    19381 cgcacagaat tactagtcgg tgaggtctta agtctggaag atgaagattt aatcgaagaa
    19441 gaagaaatcg ttattacatt gacaaacaat ggttacatca aacgtttggc aaataatgaa
    19501 ttcagagctc aacgaagggg tggacgtggt gtccaaggaa tgggtatcca caatgacgac
    19561 ttcgtcaaaa atctggtttc ttgttctacc catgatacat tgttattctt tacgaataac
    19621 ggaaaagtgt atcgggcaaa aggatacgaa attccagaat acgggcgtac agccaaagga
    19681 atcccaatca tcaatttact aggtatcgat tcgagtgaaa gcatccaggc aatcattgcg
    19741 gtgacgggag aagcagaaga aggccactat ctcttcttta cgacgcgtca aggtacagta
    19801 aaacgtactt ctgtcactgc ttttgctaac atccgaagta atggtctgat cgcaattggt
    19861 ttgaaagaag acgatgagct cgtcaatgtg ctactgacag atggtcaatc taatatcatt
    19921 attggaacac ataacggcta ctctgtcact ttcaaagaag aagcagtccg cgatatggga
    19981 cgtacagctt ccggggtcag agggatacgc ctgcgtgagg aagattatgt cgttggggca
    20041 gccttaatga aggaaaatca agaagtatta gtgatcacag aaaaaggtta cggaaaacgg
    20101 acaaaagtat cggaatatcc agtgaaaggc cgcggaggta aagggatcaa aacggcgaac
    20161 atcactgaaa aaaatggtcc actagctgga ttgaccactg tttctggaga tgaagacatc
    20221 atgctgatta cagataaagg tgtcattatc cgtttcaacg tatcgactgt ttctcaaact
    20281 ggacgttcaa cattaggcgt acgtttgatg aagatggaag ctgatacgaa agttgtcacg
    20341 atggcagcag tcgaaccaga agctgcagaa caaacagttg aagaacaagg atcagaagaa
    20401 taaccatcaa gactaagtgt gtaagctagc aagaataacc gattaaaaaa tctggttatt
    20461 cctgctggct tttttgatta gttgaatttc tcttaaaata ttcgaaactg ttcattctgt
    20521 attgcttttg accaaaatag agtgtataat tagagcttgt gagtagtcta attaattaga
    20581 taactctcct tgctcttagg aacctaagag ccgaaagtcc acagggaggt gaatatcaat
    20641 ggaaaacacg aaatatgaaa tcatgtacat tattcgtcca aacattgatg aagaagcaaa
    20701 aactgcttta atcgaacgtt tcgatgcaat cttgaaagat aacggagcag aagttatcga
    20761 atcaaaagat tgggaaaaac gccgtttcgc ttatgaaatg aacggttacc gtgaaggtat
    20821 ctatcacatc gttaaagtat cttctccatc atcagcaaat gctgtcaatg aatttgaccg
    20881 tcttgctaaa atcaacgacg atatcattcg tcacatgatt gttaaagaag aagcttaatt
    20941 gttgtttcat gtgaaacaat agaaaacgat gagaggagat gactattttg attaataatg
    21001 ttgtattagt tggacgtctg acgaaagatc cagatttgcg ttacacagca agtggtacgg
    21061 cagtcgcaac atttactttg gctgtgaatc gtaattttac aaaccaaagc ggcaatcgcg
    21121 aggctgattt tatcaactgt gtgatttggc gcaaatcagc agaaacttta gcaaactatg
    21181 ctcgtaaagg aacactttta ggtgttactg gacgtatcca aactcgttct tatgataatc
    21241 agcaaggaca acgtgtatac gtgacagaag tagtagctga caacttccaa ttgctggaaa
    21301 gtcgttctgc atctgaaaat cgccaacaaa gcggcggatt ccaaagttct ggtcaatcag
    21361 ctggcggatt tggcggaaac aataactcta atcaaacatc tcaatcatcc aacggtatgc
    21421 ctgatttcga ccgtgatact tctgacccat tcggctcaag ttcaacgatc gacatttcag
    21481 acgatgattt accattctaa acgaatttga taggagggaa atagaatggc acaacaaaga
    21541 agaggcggac gtaaacgtcg taaagtagac tacattgctg ctaaccatat tgaatatatc
    21601 gattataaag atatcgaatt actaaaaaga tttatttcag aacgcggtaa aatcttgcca
    21661 cgtcgtgtaa ctggtacagg cgctaaaaac caacgtaaaa ttactgttgc aatcaaacgc
    21721 gctcgtatca tgggcttgct acctttcgtt agcgaagact aataaatcct aggaattgaa
    21781 tgactgatcc ttttgatcag ctattcaatt cctttttttc tgtaaaaata acctctattc
    21841 gtttagtcgc tcgaatgacg aaagttatgg taaaatcaaa ttagtcatat atgggaggaa
    21901 aattatggaa aataaaaaca atcatttatc cccgaaacct ctcttcctta tcctgtttat
    21961 attaatcgaa gtattggtag tcttaatttt accatttcgt tttgttagcg cttcggtcat
    22021 tctattgatg aatttatggg gtgttcgcta ttggttgaaa atgaaacgtt ggcttatttt
    22081 aaataaacgt gaaaaaatcc agcaggcttc gactgtagct gaaaccaatc tagcttatgt
    22141 ctcagaagtc atgcctgtag gagtgattgc ttatcagcca tcaagccaaa aaatcgaatg
    22201 gctcaatcca ttagcattga aaatcaaaga tcaaagtgga ttaacagagg aagaattgct
    22261 agatactttt tttactgcaa aagaaaaaaa gaaaaaccaa gtctatctag cagataaaac
    22321 atttcagttt gatatggata cagtaaaggg tgttgtttat tttaatgata ttacccaaaa
    22381 ccagttgtta aaactccatc agacgaacag tcagccggtg attggtattt tatctattga
    22441 taactatgca gatgccattg ataaattaga tgacaaagag atttcttatt tgaactcttt
    22501 cgtcactaca ctagtttctg actggatgaa tgaataccat gtctttttca aaagaatcaa
    22561 cgcagaacgg tttttcttcg tagcacagat gatcgatctg aaaaagatga tggataaaaa
    22621 atttgatttg ctggatcgct ttcgagcaga agctgaaaaa cagagctcgc ctatcaccct
    22681 tagtatgggg attgcttatg gctcaagaac cctcgatgaa atcggtggag aagctcagaa
    22741 taatttagat gttgctttag tccgtggagg cgaccaagtt gtcgtgaaag aggtaaaaga
    22801 aggagcaaaa cctgtttatt atggggggaa atctgctaag aatgtcaaac gcacgcgtgt
    22861 tcgttctcga gcgatgagta ccgctttgcg tcggattttt gatgaaacag gcgatgtgtt
    22921 tatcatgggg cacaagtttc cggatatgga tgctttaggc tctgcttttg gtgtcagttg
    22981 tctggcacgc ttcaaccaga aaaaagctta catcgttatc aatgagaacg agatcattcc
    23041 agatgtagaa cggtgtcttg aagaaatcca taagcatcct gaattagaaa tgcaactaat
    23101 ctctccagaa cgtgccatag agctgaaaaa agaagaggac ctcctcgtga tggtggatta
    23161 ccataaacct tctctatcga tctctcaggg cctatatgag caatttgata agattgtgat
    23221 cattgatcac catcgtcgtg gggaagagtt tccaagtaaa cctctgctga cttatattga
    23281 atcttcagct tcttcagctt cagagctagt ttctgagctg atccaatacc aaagcagcaa
    23341 aacaaatcgc ttagatcgtt ttgaggccac attattgctt gcagggatgg ttgtagatac
    23401 aaatagtttc aatgtccgta caactgcacg aacttttgac gttgccagct atttaaggtc
    23461 ttgtggagca gacgcatcct tagtacaata tttattgagc tcggatttag cgacatactt
    23521 ggagatcagc aatttgattg ctggaagcga ctatgtgact tctgatattg ttgtggcggc
    23581 agggacagac gaaaaagagt ataatagtgt tatagcagca aaaacagcag atacattgct
    23641 ttcgatgatc aatatcaatg cagctttcgt cattacaaag cggacagacg gcttgattgg
    23701 gatcagtgcc agaagtaccg gcacgatcaa cgtccaaatc atcatggaat ccttaggtgg
    23761 cggaggacac ttcacaaacg cagctactca actggaaaat acgacagttg aggaaacaaa
    23821 acagaagcta tttgctgcaa tcaaagaaaa tatgaatcaa atatatgata aggagtaatc
    23881 caaatgaaag taattttttt acaagatgta aaaggaaaag gtaaaaaagg ggacatcaaa
    23941 gaagttccag caggttatgc acaaaattat ctaatcaaaa ataacttggc aaaagaagca
    24001 acgaaaagca gtatcagtca gttagcagga caaaagaaag cacaagaaaa acacgaagct
    24061 gaaatcttgg aagaagctaa aaagttaaaa gaattcttgg aaaaagaaga tactgtagtc
    24121 gaattgaagg cgaaagcagg ggaagacagc cgtttgtttg gttcgatccc ttcaaaacaa
    24181 attgctgaag ggttaaacaa acaatacaaa atcaaattag ataaacgaaa aatcgaatta
    24241 gaaaatccga tccgttcact aggctacaca aacgtacctg ttaagcttca tcatgaggtg
    24301 acggctacga tcaaagttca tgtcgtacaa gaataacaaa tcttgatagc gatgttcagc
    24361 tggtaagtat aaccagaagg aaaaaagaca aaccgaaatt tatctgagaa aacagctgca
    24421 gcaatgaaag caaaagctgc aaatcgattt cagattgatt tcggtttctt ttttgggaac
    24481 agcaaaatag cataaagaaa acgagtataa taaaagagaa ctattttttt attacacttt
    24541 cggtacgaat gatgactttt ctctcatttt cttgtaaaat gaagcaattg aagacaagga
    24601 gcgatacgta tgaatgaagt gtggcaggat cgaatcccac cgcaaaatat tgaagctgaa
    24661 caagcggttt taggctctgt ttttcttgac gcagaaacga tcattgatgc catggagtac
    24721 atagagccaa aagactttta tcgtcgtggc catcaaatta tttttgagac aatgatcgag
    24781 ttaaatgatc gtaatgaagc gattgacgtg gttactgtca aagaccggct ggaacaagcc
    24841 aatttgttag aagatgccgg tggtttaagt tatctctcag acttggcatt agcagttccg
    24901 acagcagcaa atatcgtcta ctatgcgaaa atcgttgaag aaaaatcatt gctgcgtaca
    24961 ttgatccaga cagcaacagg aatcgttacg aaaggattcg agcaaggaga agaagttcaa
    25021 acaattttag atgatgccga acgcagtatc ttggaggttt cggaaaaaag aaatcgaagc
    25081 ggctttttat ctattgctga agtactgaac tccacgattg aaaatatcga tcgactagct
    25141 caaaacaatg aagaaatcac aggtctacca actggctatc aaggtctgga taaaatgaca
    25201 gcaggtttcc agcccgaaga actaatcatt cttgctgcac gtccagcggt aggtaaaaca
    25261 gcgtttgcat tgaatattgc tcaaaatgta ggaacaaaaa cagatcgttc tgttgcgata
    25321 tttagtttgg aaatgggggc agaatctctt gtaaatcgga tgctttgtgc agaaggttcg
    25381 attgaggcca gccatttacg tacaggtcag ctttcggaag aagaatggtc gaatctgatc
    25441 gttgcgatgg ggagtctttc tcgagcaaat atttttatcg atgatacacc agggatcaaa
    25501 atctctgaaa tccgtgcgaa atgtcggaaa ctagcgcaag aaaaaggcaa tctaggcttg
    25561 atattgatcg attatttgca gttgattgaa ggtaacggta aagagaaccg tcaacaagaa
    25621 gtatcagata tctctcgcca actgaaaaaa ctggcgaaag agctgaaagt acctgtcatt
    25681 gctctttcac agttatctcg tggagtagaa cagcgacaag acaaacgacc agtgctaagt
    25741 gatattcgtg aatccggatc aattgaacaa gatgcggaca tcgtagcctt cttgtatcga
    25801 gatgattatt atgaaagaga aggcggagat gacgatgatg gaaacgagcc aaaaaatgac
    25861 aacatcatcg aagtcatcat cgagaagaac cgaagcggtg cacgaggaac agttgaacta
    25921 ctgtttatca aagaatacaa taagttttcg tcgctttcgc caagagaaga attttaaaga
    25981 agaggttgtg agccttgagc aatcccgaga gctctcgtaa gaagatgcaa ggagtaaccg
    26041 caggaacgac tagagtactt aatttaaaca gatctccaaa gggattgtga caaaagtctt
    26101 gtcacagtcc ctttttccga ataaacggtg ttcaatcagc tgacccttgg tattagctca
    26161 gcaaacg
//
LOCUS       DACEUZ010000025        25674 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.025, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000025 DACEUZ010000000
VERSION     DACEUZ010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25674
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.025"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            170..514
                     /locus_tag="IXG44_000909"
     CDS             170..514
                     /locus_tag="IXG44_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HAQ1657644.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            515..934
                     /locus_tag="IXG44_000910"
                     /pseudo
     CDS             515..934
                     /locus_tag="IXG44_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631898.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
     gene            complement(1026..1376)
                     /locus_tag="IXG44_000911"
     CDS             complement(1026..1376)
                     /locus_tag="IXG44_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1657645.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1342..2673)
                     /locus_tag="IXG44_000912"
     CDS             complement(1342..2673)
                     /locus_tag="IXG44_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HAQ1657646.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSALSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLLGIREIFDSSGKLTT
                     DFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVNN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            2929..3495
                     /locus_tag="IXG44_000913"
     CDS             2929..3495
                     /locus_tag="IXG44_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1657647.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3640..3727
                     /locus_tag="IXG44_000914"
     tRNA            3640..3727
                     /locus_tag="IXG44_000914"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3672..3674,aa:Ser,seq:cga)
     gene            4061..5035
                     /locus_tag="IXG44_000915"
     CDS             4061..5035
                     /locus_tag="IXG44_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAQ1657648.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGERKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
     gene            5360..7255
                     /gene="asnB"
                     /locus_tag="IXG44_000916"
     CDS             5360..7255
                     /gene="asnB"
                     /locus_tag="IXG44_000916"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HAQ1657649.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(7450..7896)
                     /locus_tag="IXG44_000917"
     CDS             complement(7450..7896)
                     /locus_tag="IXG44_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAQ1657650.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            8124..8273
                     /gene="rpmG"
                     /locus_tag="IXG44_000918"
     CDS             8124..8273
                     /gene="rpmG"
                     /locus_tag="IXG44_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAQ1657651.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            8361..8879
                     /locus_tag="IXG44_000919"
     CDS             8361..8879
                     /locus_tag="IXG44_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ1657652.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(8956..9912)
                     /locus_tag="IXG44_000920"
     CDS             complement(8956..9912)
                     /locus_tag="IXG44_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1657653.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            10078..10884
                     /locus_tag="IXG44_000921"
     CDS             10078..10884
                     /locus_tag="IXG44_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1657654.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            10881..11870
                     /locus_tag="IXG44_000922"
     CDS             10881..11870
                     /locus_tag="IXG44_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAQ1657655.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            11867..12871
                     /locus_tag="IXG44_000923"
     CDS             11867..12871
                     /locus_tag="IXG44_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAQ1657656.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILLSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            13003..13545
                     /locus_tag="IXG44_000924"
     CDS             13003..13545
                     /locus_tag="IXG44_000924"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HAQ1657657.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            13565..14263
                     /locus_tag="IXG44_000925"
     CDS             13565..14263
                     /locus_tag="IXG44_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HAQ1657658.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            14284..14496
                     /locus_tag="IXG44_000926"
     CDS             14284..14496
                     /locus_tag="IXG44_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HAQ1657659.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            14496..15458
                     /locus_tag="IXG44_000927"
     CDS             14496..15458
                     /locus_tag="IXG44_000927"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HAQ1657660.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            15476..15871
                     /locus_tag="IXG44_000928"
     CDS             15476..15871
                     /locus_tag="IXG44_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAQ1657661.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            16034..16399
                     /locus_tag="IXG44_000929"
     CDS             16034..16399
                     /locus_tag="IXG44_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HAQ1657662.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            16396..18207
                     /locus_tag="IXG44_000930"
     CDS             16396..18207
                     /locus_tag="IXG44_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HAQ1657663.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(18269..18436)
                     /locus_tag="IXG44_000931"
     CDS             complement(18269..18436)
                     /locus_tag="IXG44_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HAQ1657664.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            18684..19673
                     /gene="galE"
                     /locus_tag="IXG44_000932"
     CDS             18684..19673
                     /gene="galE"
                     /locus_tag="IXG44_000932"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HAQ1657665.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            19685..21169
                     /gene="galT"
                     /locus_tag="IXG44_000933"
     CDS             19685..21169
                     /gene="galT"
                     /locus_tag="IXG44_000933"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HAQ1657666.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            21193..22173
                     /locus_tag="IXG44_000934"
     CDS             21193..22173
                     /locus_tag="IXG44_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ1657667.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            22262..23086
                     /gene="tsaB"
                     /locus_tag="IXG44_000935"
     CDS             22262..23086
                     /gene="tsaB"
                     /locus_tag="IXG44_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HAQ1657668.1"
                     /translation="MYYCTSFAFGIFILRIKKYATMEGENVIAERIKYMITLGIDTAN
                     QTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAIDQLFADLQISPKDIDRIAVSDGPGS
                     YTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAANCIGVDNIIVPMFDARRKNVYAGAY
                     RWKENRLETVLEDVHISASDLFTQLQKLNEAIYFVGSDCHKFFSEIKEILPSAMINTV
                     PAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKRVEAEEKWLETHTPGDESYVEKI"
     gene            23070..23612
                     /gene="rimI"
                     /locus_tag="IXG44_000936"
     CDS             23070..23612
                     /gene="rimI"
                     /locus_tag="IXG44_000936"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAQ1657669.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LGVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            23619..24083
                     /gene="rimI"
                     /locus_tag="IXG44_000937"
     CDS             23619..24083
                     /gene="rimI"
                     /locus_tag="IXG44_000937"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAQ1657670.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            24080..25096
                     /gene="tsaD"
                     /locus_tag="IXG44_000938"
     CDS             24080..25096
                     /gene="tsaD"
                     /locus_tag="IXG44_000938"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HAQ1657671.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
BASE COUNT         8327 a         4397 c         5377 g         7573 t
ORIGIN      
        1 aaaaaaagcg ttagcaacta tgcttaaaaa gcatatcgac tgaataaact aagcattgga
       61 caggctgaaa gactacattt aaaatgaaat gcgaagaaac aatagaaaat aaagttgcct
      121 tagaaccata gttgaatcac ctaacagtcg tgaagggaga ataagagaaa tggcagaaaa
      181 acaaaccgca ggacgagatc aattaggaga ttttgcacca aaatttgcag aattgaatga
      241 tgatgtttta tttggacaag tatggtccag agaagaatta tctgctcatc agcgtagtat
      301 catcaccata tctgcattaa tttcaggagg aaattttgaa caattgccag ctcatctgaa
      361 gattggcaag caaaatggca ttactagtga agaaatagca gaaatcatta cccatttagc
      421 tttttatgta ggatggccaa aagcttggtc agcttttagt ttagcaaaag aaatctattt
      481 agaagaaaat agcgaagata agaaagaggc gtaaatgacg acaaaacacg aagaaataca
      541 caatggcgtg atttttccag caggtgaaaa aaacgaagct tacgctcaag tatttaccgg
      601 gtaaagctat ctgaatatgt tagtgaatga tcccgaagtt tctgttggtg taggtaatgt
      661 aacttttgaa ccaggttgtc gcaatcactg gcatgtccat aaagacggct atcaaatctt
      721 acttgtgacc ggaggagaag gctggtatca ggaagaagga aagccagcaa gacgattaat
      781 ggctggagat gtcgtcgtaa cgcacaaagg gattaaacat tggcatggtg cgacaaaaga
      841 cagttggttt gaacatttgg cgatcactgc tggtaattca gagtttttag aacctgtaac
      901 agatgaagtg tatgaaggat tggaaaatca ataatgtcaa gcttgaacag ctcctgaaag
      961 tagattttaa tcgaactttc aggagctgct tttttgattc agtcttttat aaatagaaaa
     1021 tagggttaat aataggaatg agaagcacgt cgaaagatct taatattgat aatcggctgg
     1081 gcatacatga tttccacgtc atcaatcaaa ctacctaaca gatgataatc ttcccgatca
     1141 tgtcggggat tacctaatag ttcttgataa tagatttcca tactagggtc ccgaggattg
     1201 ttcagcaaat cagcaagtac atctaaatct tctggctgag tttctgccgt accctgtatc
     1261 tctatgaaaa gcccttcctc agcaaattct actccaatac gtatttcagt tatttcatgg
     1321 gaatataaat aagtaagaag ctcatcgatg attctttttg ataattcttt ttccaagtgt
     1381 ctttttttcc tttctgacag cggtaaagtt atctagtatc ggtacaagga tacctgcaac
     1441 gaaaccagtt gaaaaaccac tgttatatag atttagtcct ccgtgcaaag tgctgacgtt
     1501 attgacaaga gcaatatgta gtacaccagc tattacaccg taaaaactgc cgtaaaaacc
     1561 gcttatgggc gcgagacctg tcccaaacac agctgcaaca agcatgccag tactggtgat
     1621 ctcatgaaaa gttccaaaca aagaagcaat aaatattccg actagtatcg gaaaactgtt
     1681 ttttaaatga caaccaaaag cactgaaacc caccgcagat aaaatagcac cgatgacagg
     1741 tccatttaac tgaccgccaa ccagcaaaac ataagaagac agcatcagac caactaagcc
     1801 tatgttgatc aaagtagcac caataccaga attagcaatg aaatcagttg tcaattttcc
     1861 agatgaatcg aaaatttctc gaatccccaa aagccgaaag gaattataat agaaaccaat
     1921 tccgactgtc agcgaaaaaa agccaaaaat aatccaaatg agaggaaagt gatattcatt
     1981 gaaaaccaat gtattttcga cgattgaata gctgaataac ctcaaaacag cagtaaaagt
     2041 catcgcaatc aacccagagg tgaatccaat attataaagt gtgaatccac gatggaaaac
     2101 taaagtttga gcagctaagg gcggaagaag tagaccaatt gctatgccag ctaaattccc
     2161 caataaaata cctatcagta aaggaaaacg cataccaaaa gtgatataac taacgaccgg
     2221 tgataatgcg ctaccaaaca gtccgatcat gatgtactga gaaaaaggtt tattgacaaa
     2281 tacagcgtat agatacacac ctaaaatgat tgagatagaa ttataaaggt ttttgccaaa
     2341 aaaagagaaa ccggtaactg ttagaatcga agcaaataag ggaccgttta aaatcacctt
     2401 gtgccgataa gctaaaagca cagaaagtaa ggtcaaaatc cctacattta tgaatgcgct
     2461 tccgaaaccg cctaaagcaa aataatctgt caaaagattg cttggcgagg taagaatagt
     2521 tagcaatcca ctccaattat cagtaggagt attagaaagc aagccaataa aaagaaaaaa
     2581 aacacctgta aaaccaagat aaaaatactt tcttttctgt gaaacgattc gtttttgagg
     2641 atgaatataa ataacgtcag tttcgttttc cattacacac cttctttttc attttcttta
     2701 actataactt gaaatgaaag atttggacaa ctatcattta ggtcaaaact aaaaatgtaa
     2761 gggaacccat tgaatttaac caaatttttt taattttgca aaaaaaagtt aaagaagact
     2821 gaaaaaaaat ttaaaatata acgttttgag taaaatgttt gcaaaaaatg ctaaaaatag
     2881 ggtacaataa aacaacatat actgcagtaa agaagtgggg gagttttaat gagaagaaaa
     2941 gtctatacca aggatcaaat tctaaaagct gcttatgatg ttgttgcaaa ggaagggttt
     3001 aaaggattta ctgcgagaaa tattgctaaa aagatgggga tttcgacaca gccgatttat
     3061 ctggaattca aaaacatgga agatttaaaa aatacgttgc tggattctat ctttgaacaa
     3121 ctaaaagaag atgttttccc tattgaacgt actggtgata aaatcattga tctcggtttg
     3181 aactatatct actttgcaaa aaagcatcat agtctttaca tggcattgta cattgatgaa
     3241 catggggacg gaaagaaaat gcatgatttt tcatacaatt atttccgtga aatagttaaa
     3301 gaagatgaaa gatacaatca attatcggat gaatatgtcg atgctctgca tactggtaca
     3361 tgggtagcag tgactggctt agcgtcattg atgtcatcaa acatcatgca tccaacagaa
     3421 gaacaaatca ttgcactgat caaacggaca attgactcga ttttagcatt agataatcct
     3481 gaaataatca gatagtgttc tatcagtatg aatttgatcg gtaagtccgt tggctgcaaa
     3541 ctgtcaatgg acttttttgc taaaaaaagc gatttctgtt atttatctat tagaaatagg
     3601 cttgacattt cgtgaaaaaa gtttaaaaat agtacttacg gaaggttggc agagtggtaa
     3661 tgcaccggac tcgaaatccg gcgaaccggc taataccggc gcgcaggttc aaatcctgta
     3721 ccttccttat atcaacgatt agaggttgtg aagaagctgt cctgcatcaa gtaataagaa
     3781 cggccaaaca aaaatagctt ttcctatttt ttcgtttgat tgggcttatt accgcagatg
     3841 caagcttttg aacaccgttt gttcggaata aggggctgtg acaagacttt tgtcacagcc
     3901 ccttattgtt gtacttttaa cctacaatat tttttgattc cccctgagta ctatttttta
     3961 taaatgctag taaatcaatc ttctttggcc tttttttgat aaaaaaaata ctagatgtaa
     4021 gataaaagtg taaaaacgat ttgttttagg acgggaaacg atgtgtaccg caatcactta
     4081 tgcaacgaaa gaccattatt ttggaaggaa ttttgattat gagatgtctt ataatgaagt
     4141 ggtcactatt actccaagaa attatcgttt cgattttaga aaggtaaaaa atttagataa
     4201 acattatgca atgataggaa tagcagctgg ggtatccaac tatccactct attatgaggc
     4261 tacaaatgaa aaagggttga gtatggcagg actgaatttt cctggcaatg ctgattataa
     4321 agaattacaa gaaggaaaag ataacgttgc accatttgag tttattccat ggatactagg
     4381 gcaatgctca accatagacg aagctaaaga attattggct actatcaatc tagtcaatat
     4441 cgattttagt gaaaaactac ccttgtcacc tttacactgg ttattagctg ataaagaaaa
     4501 atcgatagta attgaaagta tgaaagatgg acttcattta tatgataatc ctgtcggtgt
     4561 gttgacaaat aatccgccat ttgattatca attatttaat ctgaacaatt atcgctcgct
     4621 atcaaatgga acaccagaaa atcatttttc aaaccagatc agcttgaatg tttatagtcg
     4681 tgggatggga ggattgggct tacctggaga tctctcttct gtttctcgat ttgtaaaagc
     4741 cacatttact aaaatgaatg ctgcatcagg tgattcagaa tctgaaagta tcagtcaatt
     4801 ttttcatata ctaggctcag tcgagcaaca aaaaggagta tgcgatactg gagaaagaaa
     4861 atacgaatat acgatttatt cttcttgctg taacgttgat aaagggattt attattatcg
     4921 tacgtatgaa gacagtcaaa ttactgctgt aaatatgaat aaagaagact tggatcgtca
     4981 tgaattggtc agttatccag tcataaaaga gcaacagata aactatgtca attaattaat
     5041 gaatagatcc gtttggtatc atattttctg atacagtcat aattagacac gatctttagg
     5101 tcgtgtcttt ttttgctggt ttttttggaa atagcatcca ctaatatttt tttaacacga
     5161 agagaataag gtagcctcaa aaaagaaaac atggtaaatt agatattggg actttatttt
     5221 ctgtaaaaac agaaactttc atcttttgtt ttcacgtgac gaaattgtaa gagagaaaat
     5281 attcgtccgc tatttattct atgataagat gcatatgaat gaaataaaca gtaattttat
     5341 attaggatgt gaaagttaaa tgtgcggtat tgtaggtttt gttaacgaca aggacaacaa
     5401 aaaaacaatt attaatacaa tgatggatcg aatcgttcac cgtggaccta atagttcagg
     5461 agaatatatc gataaacatg ttgctttagg attcagaaga ttaagtatta ttgacttaga
     5521 gggcggtact cagcctattt acaatgaaga tcggacaaag atcattattt ttaatggcga
     5581 aatctataat taccagcctt tgagagaaga attgattgca gctggccacg tttttcaaac
     5641 acatgcagat acagaagtcc ttttgcatgg ttacgaagaa tggggaacag aattattgca
     5701 aaagatccgc ggaatgtttg cttttgctat ttgggataat gaaaagaatg agttattcgg
     5761 tgcaagagac cactttggga tcaaaccata ttattatgca gaaatgaacg gaacatttat
     5821 gtttggttca gaaatcaaaa gttttctgcc tcatcctgat tttaacaaag aattgaacaa
     5881 agaagcgttg aaaccttata tgactttcca atattctcca ttaaacggcg aaacattctt
     5941 caaagatgtc tatcgtttgc ctgaaggcca ttattatacg tataaagatg gaaaaataga
     6001 cattcagcaa tattgggatg cagattttga aacaaaagaa acgcattccc gacaagaatg
     6061 gatcgaaaaa atcgacgaaa ccgttcaagc ttctattgaa gcacatacgg tcagtgatgt
     6121 agaagttggt tctttcttat ccagcggggt tgattccagt tatgtaacct ctgtattgaa
     6181 accagatcat tctttctcaa tcgggtttga tgataaaacc tataatgaag cgattgaagc
     6241 cagaaaacta accgaactat tggacttaga taatacagca gcagtgatcg acggagacat
     6301 gtcgttcaaa gcattccctt tgatccagta tcacttagat gaacctgatt caaatccttc
     6361 ttgcgtacct ttgtactttt tagcaaatct tgcttcacaa agtgtgcggg tagtccaatc
     6421 tggagaaggg gcggatgaac tgtttgcggg ataccaaacg tatggattcc atacgaattc
     6481 aaaattcatc cgtgtgattg ctcaaggatt gaaaaaatta ccaaaaggaa cacgctataa
     6541 tttaggacgt aaaatcggaa aaatgaaaaa tttccatgga cggatccatt tatatgaatc
     6601 acttgctcct gcaaaagaat attttatcgg tcatgctcga gtgttcgaag aatcagaagc
     6661 atcagaagtg ttaacaccaa aatatcaaac agcaccatct gtggatgaaa tcatgactgt
     6721 tcattacgag aaaacagaag gaatcaaaga tgaagtcaat aagatgcaat atgtcgatct
     6781 tcatcaatgg atgccaaaag atatcttgtt aaaggcagat aaactttcta tggcaagttc
     6841 tttggaagtc cgagtacctt tattggatat cgaagtcatg aaattggctc aacaaatacc
     6901 aagtaagttt ttattgaatc aaaacaacac aaaagatatt ttcagacaag ctgcgaacaa
     6961 acatcttccg gaagaatggt cgaatcgtgt gaaattagga ttccctgtgc caatcaaagc
     7021 ttggttgaaa gaagaacacg gctacgaaca agtcaaagca ttattcgaag cagattttgc
     7081 aaaagaattt tttgatcaag aaaaaatcat gaagttgcta gatgaccatc acgaaggacg
     7141 taaagaagag caaagaaaaa tctggacaat cttcagtttc ttgacatggt ataaagtcta
     7201 ctttgtagat gaaacgattc cgcaagcaga agcaatcgac tatgtgactg tttgatgaaa
     7261 atcaaatatc aataaaaaag aggttttctc ctaccgttta acactgagtg ataagaaaca
     7321 atttcaaaaa acagctcttc ccgttttata gatattatga cttatggttc ggagttggca
     7381 gaggaacacc attgtttcga acataagggg gttgtgacac gattttcgtc acaacccctt
     7441 tacatatgtt caataatcga tttctttttc ttttgtccca tcttttttat aaatgaacaa
     7501 agttgattct ttgttttgtg ctacttgctt tcctttttcg attgcttctt tttttgtatc
     7561 aaaatggttg cttgcctttt ttgccccttt agatttcacg atccattgtt cttcctcata
     7621 ttctacgatc acatctgaat caagcagttt tccagcacga ggattcgtat cgtgttcatc
     7681 cgtcttgctt ggattttttt ctttttcgaa agttcgtttt tctgattcac tagcatcttc
     7741 ataccatttt tttgcttgat caatggcaat tgggatcgca cggttatcag ggtacccttc
     7801 atctaataat gcatttgcga tgtcgattgc tttttttctt gtcaatttat ctaagttttt
     7861 cattgatgca ggaaagtcct tcatgttcca tggcatatta gtatccccct ttattaaaaa
     7921 acataatcta tataaacaag tgtaagggta tttatacaaa aagaaaaaga ttttgtttga
     7981 gaagtaaaac cgttgtattt atcgtcaagt agttttactt aacaaaatcg tgaaaaacac
     8041 tagcaatcta gaaaaaatca tggtaagata atcaagtctg agagtcatct tagaggattc
     8101 ttgtaaaaat gggagggaaa aacatgcgcg taaacatcac tttagagtgt acttcttgta
     8161 aagaacgtaa ctatctaaca agcaaaaata aacgtaacaa tcctgatcgt ttggaaaaac
     8221 aaaaatattg cccacgcgaa cgcaaagtta ctttgcaccg tgaaacaaaa taatgttttt
     8281 aaagttctca attccttgct ggtcaaagaa ttgagacttt ttttatctca aaacatctaa
     8341 aatcataagg agcggataat atgtttattt ccatgtgggc acaagataaa aatggtctga
     8401 tcggcaaaaa tggtttgtta ccatggcgat tgccaaatga tatgcgtttc tttcgcgaac
     8461 acacaatgga tcgaatttta gtgatgggaa gaaagaccta tgaaggaatg ggggatcttt
     8521 cacttcctta tcgccatatc atcgtgttga caacgcaacc aaattttcgc acgaaagaaa
     8581 atgcggaagt aatgcattcg atcgatgaac ttttgtcgta tagtcaaacg atctcagaag
     8641 atatctatgt ttcgggtggt agtcggattt ttcaagaact tcttccttac actggtcgga
     8701 tctggcgaac attgatcgat agcacgtttg aaggcgatac gtatattggg aatattgatt
     8761 tttcagactt cgcccttata gaggagtatg aggggataac ggatcaagaa aatctatatg
     8821 cccatcgttt tcaaaaatgg gaaagagtaa aaaatagggt tgcttcaaaa gaagaataaa
     8881 atctatgcgt ctagtttgtt aataagaaaa aaagcatctc acgactaatc gtgaagatgc
     8941 tttttttagt taaaatcaat ttagtttttc tttgatatca ttgaccattt cctgactggc
     9001 aataggtccg ttatatagcc aactagattc aggtccgaat tgcagtacat gattgtctgt
     9061 cactgctttt agattcttcc agcttggttc attgaacatg gcagcatctt tgtcactgtt
     9121 cacaaggatg atgtagtcag catctaattc agctagcttt tcagaggaaa cagcagacca
     9181 gtcagaagtg gcttttttgg agacctcttc cactagatca ggaacaccaa attccagatc
     9241 gtcgtatacg attcttccac ttgaccgatt tttgctgacc ataaaggctg agttgttggt
     9301 cacccacaac actgcagctg tcttgttctc gattttatct gctagttctt ctcgagtttt
     9361 cttgatggat ttttgataat cggaaatgat tttttctgcc tctttttctt ttgccaaagc
     9421 tttgccgaca tcttttagtt gttcttccca tgtgacatct gttccgtttt ttacaacata
     9481 agttggcgcg attttgctat attgttcgta tttcccgcct tcgacagtag cggaagaaga
     9541 aatcaacagc aaatcaggct caaagcttaa cactttctca taaggaaggt cataagaaat
     9601 cgttgggaca tcctttaaat catcttgcag ataatcttga atggaaccac tgccaacagt
     9661 ccactgggcg ataggttttt cccctagtgc aatcagatag tcttctaagt aactgccgat
     9721 gatccgtttg ggcgcgtttg gaatctctac tttatgtcca agtgtatctg tgactgtatg
     9781 agtactattg gatgaagtcg tcgaattggc agttgtagct gtttctttat ctgaagaggg
     9841 ggagcaagcg ctcagtactc cagccagcag caatatgcta gataatgtga atacttttgt
     9901 ttttaatttc atttctttcc tcctaacata attgagaatc tttctcattg gttattattc
     9961 ctgatatttc gaacgctgtc aaccgcttca tttggctata gatatgaaag tagcgcttga
    10021 cgaaatgttg aaacagcgtc ataattgaga atgattttca gcgaaaggat gggtgaaatg
    10081 acgagattaa gagcagctga cattgcagcc ggctacgaaa acaaaaaaat cattgaaaac
    10141 atgtctgtta cgattcctga agggaaaatc actagtttga ttgggccaaa tggaagtgga
    10201 aaatctactt tattgaaaag ttttacccgt atactacctg tgaaatcagg aaagatcatt
    10261 gtcaataact ctgatatctc tacatacagt tcaaaagaac ttgcaaaaaa aattgcatta
    10321 ttagcacaaa ctagtgaaca ccctacaggt atgacagtag aagaagtagt atcttacgga
    10381 cgataccctt atcaaaaact gttcagtggc atggacacgc aagatcatga agcaattgag
    10441 tgggcgttgg atgctacagg tctagctgga tttcgtatgc gggatatcgc aacgctgtct
    10501 ggtggacaaa cccagcgtgt ctggatcgct atggcgcttg ctcaggaaac agatatcttg
    10561 atcttggatg aaccgacaac atttttagat ccggcacacc aattagagat cctacagtta
    10621 ttgactgaaa tcaatcaaat gaaacaaaca acgatcttga tgtcgattca tgacattaat
    10681 catgcttcac gtttttcaga ttatttatta gcaatgaaaa acggtaagtt gatcacagaa
    10741 ggtacacctg aagaagtgat cacaaaagag tgtatgaaaa aaatatttga gattgatgca
    10801 acgattgtca cgcttccaag aacaaataaa ccagtttttc taacctatga tttgttgcca
    10861 aaagagcagg aggatcaatt atgaggacta tgaagaaaaa tagactcctg cttttggtct
    10921 gttttgttct tttgattatt ttttttgttg ctctttcaaa tggcgcagcg actgtgggaa
    10981 aagcagattt ttggcaggcg ttgtttgatt ttgatagatc aaaacaaagt caccaaatca
    11041 tacgaaatat tcgtttgcct cgtgtggcgg gagcatttct tgtagggagt tgttttgcat
    11101 tgagcggtgc attgatgcaa ggagtgacaa gaaatccttt agcagattca ggcttactag
    11161 gaatcaacgc gggtgcttcg ttggcaatgg cccttagttt tgctttttgg cctaaagctt
    11221 ctgatttttc tgtgtttcta atgtcattgg caggttcttt tgctgtaact ttgctcgtat
    11281 ttggtttttt gcagttttcg aaaatgggca tgaatccttt acagctgatt ttagctggcg
    11341 tagctattag ttcttttttt actgctatca gccaagcact tacccaattc tttaatctgc
    11401 agcaggatct tgctttttgg tttgtaggcg gagctgccaa tatcacatgg aaacaattag
    11461 cagtattgtt tcctttatat atacttgctg ttttggtttc tttttcatta ggaaaagcaa
    11521 tatctttgat tcatttgagt gaaacacatg cattagctct tggaagtcat cctaaacgaa
    11581 cgcggttgat tgttttctgt ctagttgcag tgttagcagc cattgccgtt tcgttagtcg
    11641 gacctgtttc atttattggg ctgttggttc cgccagttgt tcgcggaatg gttggaacgg
    11701 actatcgtta tgttctacct gtctcttttt tagttggcgg tggattggtt atgttggcgg
    11761 attttacagc gaggatgatc aatccccctt ttgaaacacc ttttggatta gtcattgcat
    11821 tgattggcgt tccgttttta ctgatacaag taaggaggga acaacggtga aaaaaatata
    11881 ttggacgttc tatttcttgc tttttgtact tgtcatcctg tctataggta gtttaatgat
    11941 tggtacgcct tttatttacc tggatcagct ttttgccacg ttcataggga aagaatcatc
    12001 ttctttggcg ctgattgtta gagaatttcg agttcctcga ttactgatca gtttattagc
    12061 aggagcttgt cttggcgttt ctggttatat cttgcaagga gtaacaagaa atgaattggc
    12121 agattctagt atattgggca ttaatgccgg agcaggattt ttagtgatgc tttatttagg
    12181 tttcttttcg cactattcgc tgccgtttct attgctagtt gtagcctttc taggtggtgt
    12241 tttagctgct tgctgtgtct atcttgcggc ctatgatcga aatggctttt tgggtatgaa
    12301 tcgaatctta ttgtccggta ttgccgttaa tgccggcctt tctgcattaa cattgctatg
    12361 cacgatccaa ttatctaagg aaaattatgg atttgtgaat gcatggttag cgggatcaat
    12421 ctggggtgcg agctggcctt atgtttgggc attgcttcca tgggcggtta tcttaattcc
    12481 gctaggagga ctttatgcac agaggattgt attgctatca tttggtcaag aacgcgcgca
    12541 aggattaggc cttaacgtgg ctaaaagtca aaaaatcctt ttgttgttag ctgttgcttt
    12601 ggcaagcggt agtgtagcgg tgacaggtag tttatctttt gtaggactac tagctcctca
    12661 tgctgcgaag ctagtcgtac gtaaagaaaa taactggacc tttgttctga gcggtttgtt
    12721 cggttccatt tttctgctta gtgcagatat ggttgctaga gtgattctac taagtgggga
    12781 agtacctaca ggaattttga ttgccctttt tggtgcgcca tattttctgt atgcactttt
    12841 tatcagacaa aaacacgcgc tgaacagttg aaattcatta aaaaaggaag gccaatcatt
    12901 tctaggtctt ctttttattc tatttatgca tttatttgaa agttcgattg ctatttttct
    12961 ttttttccta taccatttta tgagagaagg aagggtgagt ttttggacca attgaaagta
    13021 agattacgcc ggaaaggaat cgaaaagctg aaaaatctgg ccaaaagtgg caagaaaaag
    13081 gaaaaagaac agcgaatcta taaacagcta ttcgccagcg agtactggaa gtcagcaaaa
    13141 gtgataggga cgactttatc gaatgaattt gaactggata cacagccggt tatccaacga
    13201 gcagaaaaag aacaaaagaa aatagttgta cctaaaactt tgccgaaacg tcagatggcg
    13261 ttttatgaaa tggatgaaca gtccgttctt gtacgttcga gttttggtgt tcttgagcca
    13321 caatcagaga agctctattt gccggagatg atcgatctcc tcattgtacc aggtattatc
    13381 tacaatagag ccggttatcg aatcggtttt ggcggtggtt attacgaccg gtatttaaat
    13441 aattttcata aaaaaacatg tagccttgtt tttcgtgaac aattgaatga acaatggcaa
    13501 ccggagagct ttgatcagaa gatccaacaa ttgtttattg actaaaatga aatgggaggt
    13561 tttcatgaat tatcaacagc aaataaaatt gcgtgcatgg ttaagacgac cgttatggac
    13621 ctatgctttt ttagggatac agacagcggt cttcctcatg atggaattat ttccgcgact
    13681 tgagatccct tattatgccg gaatgtatgg tccttatctt gttcacttca atgaatggtg
    13741 gcgtttagtg acaccaatct tcatccattt tggactgatg catttcgtaa tgaactcact
    13801 gatactgtat tttatggggc agcaaatcga ggcaatctat ggccattggc gttttttctt
    13861 gatttatatg ttcagcggga taatgggaaa tacagcaagc tttgctttta acgaagcgaa
    13921 tgttctgtct ggtggtgcaa gtacttcgat attcggtttg ttcggtgcgt tgtttatctt
    13981 agggtttcat tttaaacata acccagcgat ccagcagcta gtcagacatt ttcttttatt
    14041 tattgtgttg acatttgttt ttggtcttct ggatacctct gtagatgttt ggggacatgt
    14101 cggtgggcta attggcggat tagtgttagg gaatgttttg ggtctaccga aacaacagac
    14161 aagctattcg atacaccaaa ggattctttc tacactagtt tttgtatttc tttttgtaat
    14221 atgtatttta ctgggtttaa aaaaatatgg tttacttgta taatatgaag gaagtgctta
    14281 aagatggaga gcttatatga tgtacagcag ctgttcaaaa aatttggcat ctttatctat
    14341 gtgggtgcac gcatctatga cattgaatta atgactatcg aattgcgtaa tctgtatgat
    14401 agccgtttga ttgatcatga tacatacatg acagcgtggc gcatattaaa aagagagcac
    14461 cgaattgaag aaagccgaaa gaaaggaaat ttgtaatgga caagaagatt attgggattg
    14521 atttaggtgg aacaacagca aaatttgcaa ttttaacacc agaaggagag attcagcaaa
    14581 aatggagcat cgataccaat attttggatg atggtaagca tatcgttcct gaaatcattg
    14641 aatcaatcaa tcatcgtttg aatttatata atatgaaagc cgaagacttt atcggaatcg
    14701 gcatgggtac cccaggaagc gtggacagcg aagctggaac ggttattggt gcgtataact
    14761 taaactggac agaagttcaa tttgtgaaaa aactgatcga ggcaggaaca ggaatcaaat
    14821 tcgctatcga taatgacgcg aatgttgctg ctttaggtga acgatggaaa ggtgctggag
    14881 aaaacgatcc agacgttgta ttcgttactc taggaacagg tgttggcgga ggtatcgttg
    14941 ctggcggaaa tttgattcac ggcgtgagcg gagctggagg agaaatcgga catatcactg
    15001 tggatcctga agggttcgaa tgtacttgcg gtaaacgcgg atgtttagaa actgtatcta
    15061 gcgctacagg tgtggtacgc ctaggtcgtt tcttggcaga agaatacgca ggggattcaa
    15121 aactgaaagc aatgctagac aacggagaag aagtaacaag taaagatatc tttgaaatgg
    15181 ctcaagaaga cgatccgttt gcattgatgg ttgttgatcg cgtatgcttc tacttaggtt
    15241 tagcttgcgg aaacttgggg aatacattaa atccgtcaag tatcgtgtta ggtggaggag
    15301 tatcggcagc aggagaattt ttacgtagcc gtgtagagaa atattttaaa gaatttactt
    15361 tcccgcaagt aagagaatca acaaaaatca aattagcaga gcttggtaat gaagctggtg
    15421 tcattggtgc tgcctcatta gccttgaaat ttgcttaaaa aaacggaggc attacatggt
    15481 tttatgggta attaatatca ttttgctatt gattgtgctt gcaatcatcc tttgggaagt
    15541 ctatctacga gtaatggcca aacgttcagc aacgactttg acagaagaag aatttcgaga
    15601 aggaatgaga aaagcacaag taatcgatgt tcgcgaaaaa gatgtgtttg atgctgggca
    15661 tattttaggt gcaagaaaca tcccttacac tgttttgaaa gattcattag gatctattcg
    15721 aaaagaccag cctgtctata tttacgatca aaagaaaagt ttgagcatcc gtacagcaaa
    15781 taaattacgt aaagcaggtt atcaagagat ttatttactt aagggtggat atgacggatg
    15841 gtctggaaaa atcaaaagta aaaaagtata aaacagatga ttgtatgaca gctttaaaaa
    15901 ccgaatcaat cattagattt tgaccaagat cacatgaacg gcgcaatgga tagttgtgcc
    15961 gttcattttt tttattttgt gctattatct acatatataa gctaagcgaa aaggtagaaa
    16021 gaaggaaaca actatgatca aattaaaacg tgtttacctt gatgctgaaa aagaggatgg
    16081 ctatagaata ctcgtggata gattctggcc acgaggaatt tcaaaagaaa aagcgcagtt
    16141 agatctgtgg ttgaaagaaa ttgcacctag taatgaagca cgcaaaactt ttgaccatgt
    16201 accagaacga tttgcttcat ttgcaaagaa ataccagcaa gaattgattt tcgaagaaga
    16261 aaagaagcaa gctattttta aactcagtca acttatgaaa gaacatccag tcattacttt
    16321 tgtttatggt gcaaaaaatg aacatcagaa ccatgctgtt ttattgaaaa aatttatcga
    16381 aatggaggca tgcaaatgaa aatagggatc attggtgccg gacctagagg attaagtgta
    16441 gcagaaagga ttctccataa taatcaggac agacattctc ttgaactttt tcttttcgat
    16501 cccaatggtc ctggaggtcg agtttggcgg ttgaatcagc caccggagtt attaatgaat
    16561 tctgtttctc aacaagtgac gctttttaca gatgaaacat tgtccagtgg aggattggta
    16621 acacagggac ccaacttgtt tcaatggagt aaaggacctg ctacagatta tatcgaacag
    16681 acggtaacca aaaataagca actaattata gaagaaatca atcgtctgga aaaagatcag
    16741 caatctactc gatgtttata cggactctat caacgctggt ttttctcaaa gttgcaagaa
    16801 gaatttccag cttctattca gtttgtttat tctttagtaa aaaaagtaag caaaagaaaa
    16861 gatggttatc ttttacagac agatcagcaa agctattcta tcgatcagct tgttttagca
    16921 agcggtcact gggaaaatga tgagacagaa gaagaaagag tgtgggtaga ctacgcaaag
    16981 aaaaatgttt tactgtatca gcttccttct aatcctgcag atgtagatct ttcgaagata
    17041 ccgcctaaag aaaatgtctt tttaagaggt ctaggcttaa gtttttttga ttatgtcgga
    17101 ttgtttacag tatcaagagg agggcgcttc gagaacgaaa agggaaaact ggtctatcat
    17161 ccatcaggta aggaaccgat tgtatacagt ggatcaagaa gggggctccc ttattaccct
    17221 cgaggaagaa atcaaaagca aggcggagca atggcatggc cacggttact aacaaaagaa
    17281 aacctggagc aatggcatca gtcgggattt ttgactggcg aaacattttt tgactatctg
    17341 aaaaaagacg ttgagttgtt ttattacaag aaaatcatcg aagaatatca cttgccgatt
    17401 ccaaagaaaa attttgagca tgattttctc tccctctctt cagaggagtg tctcggaaag
    17461 catccagcac taatccctta tagatgggat tggtcgtatt tagaaactcc tcttgcctat
    17521 cagaatgagc ccttttcaga agctagccga atatttattg aagggcagat aaaagaagcg
    17581 gaaaaaggca attgcacagg agcatttgct tcaacatttg atgcattaaa agactggcgt
    17641 gatccaatcc ggcaggctat tgaatggcac gttttttctg ctaaggaata caaagaaata
    17701 ctatggggat ggtttactca tctaaatagc tttttaacga tcggtccgcc aatcattcgg
    17761 acgaaagaat tagcagcact gatagatgcg ggtatttttc atttgatcga accgccattt
    17821 gatattcagc aaaagcacaa ccgctatgtt atttctctac aagataaaga aatcagcagt
    17881 cgctatttga ttgaagctcg attgccacaa acaagctttt ctcgttcaag taatcctgtc
    17941 ataaaaaatt tgaaggaaaa caaacttgtt cgcccttttt cattttcaga taatacagga
    18001 agttatcatt cagaagctat agatgttgag cgaggtacga atcgcgtgat tgatgcttca
    18061 ggggaaatcc agtctgactt ttattgctat ggaatacctg ttgaaggtgt ggattggcta
    18121 actgcagcag tagctagacc ttatacagat ccatggaatc tgagacaggc agataaaata
    18181 gcacaactta tattgactga caaataaaaa tacgcatgat gacttatctg ttttttagaa
    18241 agtcttcatg cgtattttta aataattatt aacgagaaac gcgtttacgc attgcttttt
    18301 tacggttttc ttcgatcatt gcttcttttt cgtcaattgg atcgatcact gtttttttca
    18361 cagaagcaac caaaccagca acggcagcgg ctgttgccag acttcctact aatattccag
    18421 atacaaattt tttcattgaa tccacctcat ttattatttg ttatatctat tatggaacaa
    18481 aagagaaaaa aaggctagtt ttacgtttgt ttgtaaacct ttgcaaaaaa tccttgaaaa
    18541 tgtttactaa atgttttatt ataaatttac catatgaatt tacgaaattt tactttgttt
    18601 gttttcacca taaaagtaaa cttcgttttt taaattatat agaatcaaca aacacaattt
    18661 tactaatcaa aggagtgttt tttatgacaa ttttagtact aggcggagcc ggttatatcg
    18721 gttcgcacgc tgtagaccag ctagttcaaa aggggtatca agtagctgtg gtagataatt
    18781 tgctcacagg tcacaaacaa gcagtacatc cagatgctca tttttatgaa ggagacatcc
    18841 gggataaaga atttctacgt tccgtatttg aaaaggaacc gattgaagga gtaatccatt
    18901 ttgctgcaag ctctttagta ggagaatctg tcgaaaaacc attgatgtat ttcaacaaca
    18961 acgtatacgg tatgcaaatc ttacttgaag tcatgcacga gttcaacgtg aataaaatcg
    19021 tattctcttc tactgcagca acttatggag agccgaaaga gtctccaatc acagaagata
    19081 caccagctaa tcctaaaaat ccgtatggtg aaagtaaatt gatgatggaa aaaatgatga
    19141 agtggtgtga ccaagcatat ggcatgcgct atgtcgcttt gcgctacttc aatgtagccg
    19201 gagcaaaagc agatgcatct atcggagaag atcatacacc agaaacgcat cttgtcccaa
    19261 tcattttaca agtagctctt ggtcaaagaa aagccttggc tgtttatgga gacgattatg
    19321 atacaccaga tggaacatgt atcagagatt atgttcaagt agaagatttg attgctgcac
    19381 atattttagc tttggaatac ttgaaagaag gaaatgaaag caacttcttc aatttaggca
    19441 gcagcaaagg atactcagtc aaggaaatgc tagaagcagc tagagaagtg acaggaaaag
    19501 aaattccagc agaaatagca ccaagaagag ctggagatcc tagccgatta gtcgcttcaa
    19561 gtgaaaaagc gagagagatt ctgggctgga aacctgaata cacagatatc aaagcgatta
    19621 tcaaaacagc atgggattgg catgtcagcc atccaaatgg ttatgaagaa taggggaatg
    19681 agatatgtca atcagtcaaa cgatttgtga ttttgccaca cttgcgatcg aagctggcgg
    19741 atggatggaa atggaccgct tatatttaca gaatcgtctt ttagggatgg taggagaaga
    19801 tgctctagaa agcattgaag taagaccagt taaggaagaa tctgtagact tattggatca
    19861 gcttgtcgct aaggcaagag aaaatggtgt tctttcagaa ttgccagcag aacaagagat
    19921 ttttgaagcg caattaatgg attttctcac accgccacct tcagtcgtca atgcattctt
    19981 tgcacagcac tattcgaaaa gcccagagga agcaactgag tatttcttta atttaagtaa
    20041 gaaaaatgac tacatcaaaa caagagccat cgcaaaaaac atcaaatttc cggcagagtc
    20101 ggagtatggt gaactggaaa tcacaatcaa tttatctaag ccagaaaaag atccaaaaca
    20161 aatcgcagca gaacgcgagg cggtaaaagt agactatcct acctgcatgc tttgcatgga
    20221 aaacgaagga tatagaggtc gcttgaacta tccagcccgc accaatcacc ggattattcg
    20281 aatgaatcta gacggtgaaa gctggggatt ccaatattcc ccttatgctt attacaatga
    20341 acattcgatc attttatcag aacaacaccg cccgatgaaa atcacaaagg aaacatttgc
    20401 tagactgctt cggattactg aagtcttgcc tcattacttt gttggttcga atgctgactt
    20461 accaattgtt ggaggatcga ttctttctca tgatcattat caggcaggac gtcatgaatt
    20521 tcctatggca aaagcaccga tagaaaaact tatcaaacta gaagattatc cgcaagtcat
    20581 cgcaggagtc gtgaaatggc cgatgtctgt gatccgtttg caatcaacag agaaaaactt
    20641 gcttattgaa gcagctaact cagtattgga aaaatggaaa gcatattcgg atgaaacagt
    20701 atctatcaga gctttttcag cagatggtac accacaccat acgattacgc caattgcacg
    20761 aagaagggac aattactttg agttggacct tgtccttcga gacaacaatg tttcagaaga
    20821 acatcctgat gggatttttc atccacataa agatgtccaa catatcaaaa aggagaacat
    20881 tgggttgatc gaagtaatgg ggcttgctgt tttaccacct cgtttgaaac cagaactagt
    20941 agaagtagag catttcttat taggagaaga aaaccagatg gctgaatatc atcgtccgtg
    21001 ggcagaacaa ttaaaaaaag aacatcctga tttgacgaaa gatacggtca ccgatgtcgt
    21061 acaaaaatct gtaggtcagg tttttgcacg tgtactggaa gacgctggag tattcaagcg
    21121 agatgaaaaa ggacaggcag cgtttgaacg atttactgcg attttataaa cgtaaaaaag
    21181 gaggagagac aaatggcaac aattaaagac attgcgcagt tggcaggagt ttctcctgct
    21241 actgtttcaa gagttctcaa ctatgatcct gatctttccg ttggcttagc cacaaagcaa
    21301 aaaatttttg aagcagcaga acgcttaaat tatacgaaac atcgtaaaaa tgcagcaaat
    21361 actaagcaaa cgcttcgcct gatccagtgg tacgacgata cggaagagct tgaagatctt
    21421 tattatttat ctattcgcct aggaatcgaa aaaaaggcag aagaagcaaa cgttacttta
    21481 ttgaaagaaa cgtgggattc cattagtgat cttccggcag atggaacgat cgcactagga
    21541 aaattcgacg aagctcagtt attagaaatg aaaggatccc aaaatgcctt gcttttggta
    21601 gattctgacg gcacttctca aggaatcgac tctctagttg tcgactttaa gtcaagtgtt
    21661 caaaaagtca ttgaacactt tgtctcccat aaagcgcaac atatcgctat gcttgctgga
    21721 acagaatata cgaagaaaaa ccatcagcgg ctaaaagatc ccagaattat ggcatttcaa
    21781 caagttttgg cagaaaaagg ctacgacgaa ggtccaatta tcgaagcaga tttttctgtc
    21841 gagtcaggat atcaagcagt gaagcagtat ttaaaaacga atccgcatgt tcctgatgcg
    21901 ctttttgcag ctaatgatgc attagcgatt ggcgcactga aagcgattca agaaaaaggt
    21961 ttagtggtac cagaagatat ttctgtgatg ggcttcaatg atgtcagtgt agcgaaatat
    22021 gtaacgccct cgctttctac tgtaaaagtt tataccgaat ggatgggaga gctagcagtg
    22081 gagacgattc ttcaattaag taattccaaa gcaccggttt caagaaagat tattgtggaa
    22141 acagagttga ttattcgaga atcaacgaaa taaaagatgg aaaagctgtg ggaaaatgga
    22201 gtgataccgc agcttttttg tgtttcagga aggggcaaaa tttgcccgcc agcagataat
    22261 catgtattat tgtactagtt ttgcctttgg tattttcatc ctcagaataa aaaagtatgc
    22321 tacaatggaa ggcgagaatg tcatagcaga aaggatcaaa tatatgatta cattaggaat
    22381 agatactgca aaccaaactt tggcgatcgg tgtggtagaa gacgaacaaa ttcttggaca
    22441 aatacaaaca aatatcaaac gcaaccacag tgtaacactg atgcctgcaa tcgaccaatt
    22501 gtttgcagat cttcaaataa gtccaaaaga cattgaccga atcgcggttt ctgatggtcc
    22561 cggttcttat acagggcttc gtataggtgt aacgacagca aaaacgattg cttatacatt
    22621 agataaagag ctagtaggag tatctagtct gaaaaccatc gcggctaatt gcatcggtgt
    22681 ggataatatt attgtgccca tgtttgatgc tcgaagaaaa aatgtatacg ctggggcata
    22741 ccgctggaaa gaaaatagat tagaaactgt acttgaagat gtacatattt ctgcttctga
    22801 tttgttcaca caactacaaa agctgaatga agctatttat ttcgtcggga gtgattgtca
    22861 caaattcttc tctgaaataa aggaaatatt gcctagtgcg atgattaata cagttccagc
    22921 atgggatatt ccaagtggtg taactgtggc tcaattaggt gctttagttg aaccagttgc
    22981 agagaaacaa gcttttcttc ctcgctattt aaaacgagta gaagcagaag aaaaatggtt
    23041 ggagacgcac acacctggag acgaaagtta tgttgaaaaa atttagagcg tatgccagaa
    23101 gcttttttca agataaaatg atttatacag aaaaaacagt cgaacaaatc gatcgtcgtt
    23161 acgtactaag agaattgact acagaagata tcaaagagct gctgggtgtc gaacgagaag
    23221 tctataatgg cgaattgcca tggacaaaaa gtgcctttat atcagaattg aagtcaccgc
    23281 ttcctcatct atatctaggt atctttgata atggaagaat gattggtttt atcggttcaa
    23341 gagcgttcgg tttagattgc catatcacga atattgccgt tcgaccggca tatcagcgta
    23401 aacgaatcgg ttcgctgctt attgacgaaa tcgagaactt tgctataatg aatcgctgtg
    23461 agacgatgtc attagaagtg cgtatgagta atcaagatgc acaaagactt tatcggaaat
    23521 tggggtttca agcaacaacg atcaaaaaaa gatattacac tgaaacaaaa gaagatgcgt
    23581 tggatatgat aaaactttta gaaaatgagt gagattagtt gatttgcaga aaaaaagatt
    23641 ttgatgaagc gtacttggca gaatgcgtat ggatgattag cgaaaatgct tatccacatg
    23701 gttcaccttg gacgaaaatg cagtttttgg cagacatcca acagcctcac tctgattatc
    23761 tgcttttagc tgaagataac cagatctgtg gctttattgg atactcgaaa gtaatagatg
    23821 aagtcgagat cacaaatatt gctgttgcag tttcagaaca aggaaaaggg catgcacggc
    23881 atttgttaca attactgatt acggaacaaa taaaagattc gctgcatgtt tttttagaag
    23941 tacgtctatc taatgaagca gcaagaaaac tttatgaatc agaaaagttt cgtatattag
    24001 gtaaacggaa atcatattat cgcaatccga cggaagatgc gatcattatg agcagaaagt
    24061 taaaaacgga gacgatgaaa tgaatgaaga attgattctg gcgattgaat caagttgtga
    24121 tgaaacgagt gtagcggtcg ttagaaatgg aacagagatc ttatctaaca tcgttgcttc
    24181 tcaaataaac agtcataaac gttttggtgg cgtagtacct gaagtagcta gccgccatca
    24241 cgtagaacaa atcaccttat gcttggaaga tgcactggta gaagctggtg tgtcggcaga
    24301 agatttatcg gcagtcgctg tcacttatgg accaggactt gtcggctcgt tgctgattgg
    24361 tatttctgca gctaaggctt ttgcttgggc acaccaattg ccgcttattc ctgtaaacca
    24421 tatggcaggg catatctatg cagcccgtct ggttaaacca ttccagtttc cattgatggc
    24481 acttcttgtt agcggaggac atacagaatt agtctatatg caagaagatg gatcatatga
    24541 aatcattggg gagacaagag atgacgcagc aggagaagct tatgacaaag ttggaagagt
    24601 attaggtctt tcttacccaa gcggaaaaga aatcgatcag ctagctcatc agggaaaaga
    24661 taattaccat tttccgcgag cgatgatcca tgaagataat tatgatttca gttttagcgg
    24721 attaaaaagt gcatttatca atttggtgca taatgcacaa cagcgtggag aagatctaga
    24781 caaaaatgat ttagcagcta gctttcaagc aagtgtgatc gatgtattga ttactaaaac
    24841 acttcgagct tgtcaaaatt atccagtcaa gcaactgatt atagctggag gtgtagcggc
    24901 caatcaaggg ttgcgtgaag gattgcagga tgctttggca actaaactcc ctgaggtaga
    24961 actagtgatt ccccctcttc gactttgtgg agataatgct gcaatgattg gagcagcagc
    25021 acatgtggaa atgcaaaaaa gaaattttgc tagctatcag ttgaatgctg atccaagctt
    25081 ggttttatct gaataaagag gttgtgaaaa agctgccctg catcaagtaa taagaacagc
    25141 ccaggaaaaa cggcttctca tgtttttgac ctggctgttc ttattaccgc gtatgcaagc
    25201 ttttgaacgc cgtttaatca agagttcgta agcctgtcgt ttaactctga ttcacaagga
    25261 acaatttcaa aaaacagctc ctcatgttta tgaacattat gacttgtgac cgaagagttg
    25321 acaggtacac accgtttaat cgaaataggg ctgtgacacg tttttgtcaa agccctattt
    25381 tttacatact tttaatcatt attgttattt tgctattttt tacagcttat ttatcattat
    25441 ccgttcctct gattgccctc actgagtaat cgtttattgt cgaggaatat cgaataatct
    25501 agatcggatg aggacaagac gcttcatcta gttaatctaa cagtattttt tgcaaacagt
    25561 gaatattaca taactgaaac acacatagaa aatctttgag tcttgtccag tgatctaaaa
    25621 ctgccggatc tttcagaagt tttagggagg aaaataagca gtgaccctga tatt
//
LOCUS       DACEUZ010000026        25549 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.026, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000026 DACEUZ010000000
VERSION     DACEUZ010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25549
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.026"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(159..1469)
                     /locus_tag="IXG44_000939"
     CDS             complement(159..1469)
                     /locus_tag="IXG44_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAQ1657672.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1481..2899)
                     /locus_tag="IXG44_000940"
     CDS             complement(1481..2899)
                     /locus_tag="IXG44_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1657673.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3139..4707)
                     /locus_tag="IXG44_000941"
     CDS             complement(3139..4707)
                     /locus_tag="IXG44_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HAQ1657674.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(4711..5121)
                     /locus_tag="IXG44_000942"
     CDS             complement(4711..5121)
                     /locus_tag="IXG44_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HAQ1657675.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(5118..6041)
                     /locus_tag="IXG44_000943"
     CDS             complement(5118..6041)
                     /locus_tag="IXG44_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HAQ1657676.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(6038..6916)
                     /locus_tag="IXG44_000944"
     CDS             complement(6038..6916)
                     /locus_tag="IXG44_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAQ1657677.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(6903..8240)
                     /locus_tag="IXG44_000945"
     CDS             complement(6903..8240)
                     /locus_tag="IXG44_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAQ1657678.1"
                     /translation="MYDILTFGASIAELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8243..9346)
                     /locus_tag="IXG44_000946"
     CDS             complement(8243..9346)
                     /locus_tag="IXG44_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAQ1657679.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9420..10928)
                     /locus_tag="IXG44_000947"
     CDS             complement(9420..10928)
                     /locus_tag="IXG44_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ1657680.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(10894..11586)
                     /locus_tag="IXG44_000948"
     CDS             complement(10894..11586)
                     /locus_tag="IXG44_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAQ1657681.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11608..12582)
                     /locus_tag="IXG44_000949"
     CDS             complement(11608..12582)
                     /locus_tag="IXG44_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1657682.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            12882..13754
                     /locus_tag="IXG44_000950"
     CDS             12882..13754
                     /locus_tag="IXG44_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1657683.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14212..16410)
                     /locus_tag="IXG44_000951"
     CDS             complement(14212..16410)
                     /locus_tag="IXG44_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1657684.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEIEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16642..17634
                     /locus_tag="IXG44_000952"
     CDS             16642..17634
                     /locus_tag="IXG44_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1657685.1"
                     /translation="MVTIKDVAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17694..19115
                     /locus_tag="IXG44_000953"
     CDS             17694..19115
                     /locus_tag="IXG44_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HAQ1657686.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSLPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19108..20607
                     /locus_tag="IXG44_000954"
     CDS             19108..20607
                     /locus_tag="IXG44_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009224330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HAQ1657687.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20646..22040
                     /gene="uxaC"
                     /locus_tag="IXG44_000955"
     CDS             20646..22040
                     /gene="uxaC"
                     /locus_tag="IXG44_000955"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAQ1657688.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            22199..22882
                     /locus_tag="IXG44_000956"
     CDS             22199..22882
                     /locus_tag="IXG44_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HAQ1657689.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            22884..23720
                     /locus_tag="IXG44_000957"
     CDS             22884..23720
                     /locus_tag="IXG44_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAQ1657690.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            23731..25485
                     /locus_tag="IXG44_000958"
     CDS             23731..25485
                     /locus_tag="IXG44_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1657691.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
BASE COUNT         7792 a         5199 c         4485 g         8073 t
ORIGIN      
        1 acgacttagt tatcttatca cgtctaaaaa catgtgtcaa ctcttttttg gaattttcat
       61 aaaatgtttc aatgaagcgc gacaacgttg tttaatatat cattaaaaaa aataaaggtc
      121 aagctaaaat attatttaag cttgacctta ttataatttc aacgattgtc aaaagtattg
      181 tttgcagctg tgttgccagt ttctatagat aaatcacagc ttttgaaaca taagttttgt
      241 actgcttcga ttcgcccgaa ttcttttgca gttatttggc aatgagaaaa gcagacatct
      301 tctataggct tttcaggaaa tgctactaaa tcaaaagctc gggaaggaag tgagtaacct
      361 ggagaaaggc atgcctgtac gtttttgatg tgtatgtctt tgactttcgt catttgcttt
      421 gattcaggta tttcttcagc cactgcttgc caactttctg gcatttgagc aaggtcattg
      481 ctattttttc tattgtattc attatgccag tccattatcc aagaaaaagg aaattgtaca
      541 tcaatcatat tcagattttc taccctgata ttttcgatca ctccccctct ttcttttgaa
      601 gatttcattc gaataccaca gtctgactga aaaaaatcga tatcgtgaat atatacgtca
      661 ctaactccag cactaacttc gctaccaata gttacaccgt atcctgaacg gattttacat
      721 ctagcaacct cgattcttga tgattttcga ttgactcttg caccattgcc atctctgcca
      781 gacttaatag caatacaatc atcaccacag gccaaatcac attcatagac acgcacattt
      841 gttgaagaat caatatcaat cccatctgta cttggtccat cattgttgcg gatgattact
      901 ttttctacta atacatcgtt agaataagtg atttgcaagt tccaaaaacc tgatttctga
      961 aaaatcaaat cacgaacaac aacatgttct gcatgataca gtaaacaagc gcgtggacgt
     1021 ttaatagcat aatccgcaat ccaacgaagt cccttttgat cgtattcagc tcttgtcccg
     1081 cttttttgat cttttcccca atacagctcc caccaatggt ctccttgacc gtcgattatt
     1141 ccttttcctt ctataagaat atctttcgcc gagagaacat tcaaaatcgc tgctggccat
     1201 ttcatttcta cgccggctac tcgtgtatcg atttctggaa atgcttcgat atctgttctc
     1261 cccttgagga ctgcaccttc ttcaaagata agatgactct tatcttttaa aaaaagggct
     1321 ccgactaaat aaatgccttt tggaataaat acaggtaagt gttctttcgc accttgatcg
     1381 attgcttttt gcagtattat tgtatcatct tgttgagtgt cgtttgtttt aaagttcaaa
     1441 gcattgataa tccctttttt tgtccgcaat acgtttctcc ctatctttca ttgatttttt
     1501 tcaattcttc accaataaat acagtttttg aacgattcag tgggtaacct actagcaaaa
     1561 tgattgcgga tacaaataag cagaatgctg gtaacaagtt tgccatcgta taaatccttt
     1621 cttgtactaa aactgattgg atggctccac ctgttgtaga cgattggtaa ccaatgagtg
     1681 ccagcataaa tccgcctacc gctcctgcac atgcttgtcc tacttttctc gcaaaagaat
     1741 ttactccata tactgttcca tcttctctta agcctgtctt atattgatga taatcaatca
     1801 cgtcagtaat gaatgcccag accatcaaat tgaataaacc agatcctaaa gtagcgataa
     1861 ataaaagaat cagatatacc catgcatttg ttatatgaag gaaatacatc agcaagtaca
     1921 tacatgaaga agcaagtaac gcaaatacgc ttgcttcctt tttaccaaat aattttgtca
     1981 gctttgtagc aaatggtgct aaaactacta ctgttccgta agtaaataaa agagcaacag
     2041 acattgcttg tttattatgg aagtaatcat tgaataaata agtcatattc gtcccagcta
     2101 aaatctgatt gatcacaatg aaaatgtcaa caatgactaa aataatcagt gctcgattag
     2161 ctaacaaacc ttttaccaac tgagaagcag atactttttc gtttttgaca actcttactc
     2221 tttcagtagt caatttacaa gttaacatat atgcgacaaa tgctattgca gcacatccga
     2281 ttgaaatcca aaagaaatga ttccctgaaa cgacttgttg tccactcttg ttatgggtat
     2341 acattaaaat cggaatgaag aatcctgtag ttgatccgcc aatcgcagaa ccaatgcttc
     2401 ggaacgtgga caacgaggtg cggtcattag gatttgagct aattgctgct gccattgctc
     2461 catacgggat attgattgtt gagagaaaaa tcccataaac aatgtatgaa acaaaaatat
     2521 aagcaatctt caccgtcatt ggaagatgtt ggacgaatgg caaaaacaaa atgacagtca
     2581 ctaagcaaaa tggatacttc atttttctga tccaaggaat aaatcggcca gtttctgtta
     2641 actctgagac atcacataat cttccaacca tcacatcagc aaacgcatcc aaaattctcg
     2701 aaatcaagaa taacgtacct acgattgccc ctgaaacgcc aagaacattc gtataataaa
     2761 tcatcaagaa actattgact aaacctaaaa taaaacagtt acctagatca ccgaacatgt
     2821 agcctatctt atcggaaatc ccaaattcct ttttttcttt ttttattgta tttgctgtaa
     2881 cagcattttg tgaaagcatt tgaattcctc ctacctcttc tagtaaacgt tttcaaaatt
     2941 ataactcttt ttttcttgtt tttatattca caatgttggc tttttattgc attatcttga
     3001 cagataaaaa acaaaaaata atctaaaaac attgatgcat aagattgata acttttttta
     3061 cataacaata tatacaaaca aaaaaattca ctaactagtg aaaataagtt agtgaacctc
     3121 atttattaaa gaattaaatt acagatgatc ttttccatca tacttgaaaa actcaaaatc
     3181 agcataactt cctctttttt tatttagatc atgcactgct atcccgataa aatttccagt
     3241 aaaccctcct gagagtatac acatgttttt ttgcggacct actttcttcc attcttcagt
     3301 tgatacgtct ttcaaatgaa actgtgctgt ctctttcttt acagtgactt tcaaaaaagt
     3361 cttcttttct gtgagtggaa tcgtccaatc ttctatttgt gctactccgt tttctgattc
     3421 tatcatacgt aggcattttc ccttgtcctc attccaagta acataacaat aaatataatt
     3481 ggattcattt aagtacagta ccagtccagc catctgatta aaattattag gcatgtattc
     3541 aaatgctgtc tccgcatcaa aagtaaaatc cgtttgccga atagctaaca aatgatgttc
     3601 gaaggtagat tgaagagatt caccggaaat cagacgtaaa aaacctggtc tacattttgt
     3661 atcacaccaa tcagcatttg gcaaaatgcg tctggcattc cagttcttct ttagctctcc
     3721 ttcaaatgta tccataaact gatgatcttt ttcttgttgt acctcttcat ttgtctcaat
     3781 cattatttct tccatcggag ttgtctggct atctttaagt cttaaccaat cgtcggacgt
     3841 ccaatacact tcttgaatag cggtttctct tcccaaaata gccgcaccgt ttaatggtct
     3901 ggtacaaaga tatgtcatgt accatcttcc tgacggggtt tgtactaagc tcccatgtcc
     3961 tgtacattgc aatgtagaag caggtttgtc actagctgtc agcataggaa aaccaggatc
     4021 aagctcgtaa ggaccagtaa tattctttgc tcggcaaact gtaactgaat gtcctcttcc
     4081 tgtaccgcct tctgccgtca tcaaataata atacccgtta cgaaaataca aatgtggtgc
     4141 ctccgttttc gctaattctg tacctgcaaa tattttatag atgggaccat caagcttctt
     4201 cttctctgca tcatattctt gtaatacgat accgctggat ttattcggtg tgtccagacg
     4261 ataatcccat agttcattga caagccattt ttttcccgtt tcaagatcat ggaacaaaga
     4321 aggatcaaag ccgttactat ttaaataaat tggttcggac caaggacctg tgatctcaga
     4381 tgcagtgatc aaataattgt ggacatcttt aaacggacgg cctgcacttt tgacatctgt
     4441 gtaaacaaga taaaacaatc catcagcata actgatctgt ggcgcccata tacttccatt
     4501 tgtaggattt ccttgcaaag atacttgagt ggtcaaaata tccgttgtat gtgaccagtt
     4561 gactaaattt ttcgactcat atactcggat tcctggaagc cattcaaaag aggagacaat
     4621 aatgtaatac cgctctttca ctcttaatat attaggatct ggattgaatc ctcttaaaat
     4681 aggatttttc actttgatag tctgcatgat tcaggctcct tttctcattc ttactcgata
     4741 taaactgaat gccccagctg cgaaataaaa aaatgcaaaa ggaaacgcaa taaactgagt
     4801 gccaatcaac aatatgaatc ctgttataaa atacaaacca gtggcagttg ttaagtattt
     4861 tcctttagca gtcaaaaaca atgctactgc taaaaaagca agtgaaataa acaacacaac
     4921 ggcaaacaga ccagtcatgt cctgtaataa tccaaatgta ttttctaaag acaaagttga
     4981 atgatctaca gcaagcgact gataactttt tttaaactct gacgcagaca tattacttaa
     5041 agtaactgct aacccgccaa agaaaatcaa agatagactg ccaccgacca ttcctagcaa
     5101 tagttcccat tttcttttca tttctgaacc ccctgctttt ctaaatgtat ccattcaggt
     5161 cttttgatcg atgtttcata attgttatta cgttcagaga atttgacagt ctgttcattt
     5221 ttcttgtcat cccaattatc ccaaccagtt gggtgaacat gcccacctat cttacacgct
     5281 tcaaataaga catttgcata cggacgccac ggtcttccta aatagacgtt tctagtatca
     5341 ggttctgcag tcaaggtaca gctttcaaaa cgataccctg ttttttgatt tttcggcgta
     5401 gatgctgctg tgatgtatcc aggattttta ctggctaagc tatggatatg acactgatga
     5461 aaaacggcat ctgccccacc aaatatgaaa tccaccgttc cttcaatcca gcaatattca
     5521 taggtctgac gacaatactc aggattggca gaagccggtg tacaaaaagg agtaccatct
     5581 ttttgcaaat caggcaacgg acccgtgcat aaggtatcct gataaccaga tagtcgacag
     5641 ttttttaaag atacgcgatc tcctgaattg aataaagcaa cagcctgtcc taccttttct
     5701 cctggacctg ctgtattttc gatgtgaaga ttttctaata cgacattcga tccttctaag
     5761 aaaaaagtag cacttcggaa agtccctaac tggctaccgt caagatactc ctgttttgca
     5821 aaacgattac cggagataac tactgttcct gctcccacaa gtttaaaatt ggataatctg
     5881 ctttctatat attcatcata tcttccttct tttactatca agacctttgg atggctatcc
     5941 ggcaggcctg ccaaataatc gattccttct tggatagttt caaaatccgt cggggtatct
     6001 atagcattct ttccaattat cagtttgtct ttcatcctca taaatgctcc cttatgaatt
     6061 gtttccaaat atttctcatt tcttgtgttt ccatatgacc gcctgttaaa gaaaaagaag
     6121 aaaagttttc tggactaccc attgattgat actttttgtc cagccctttt ctcagcttca
     6181 gtacgccttc gtccaagcac atcgtatcgt tttttccaac taagctaaga cgagggcgcg
     6241 gagcgatttt ttcttggatt gctaacgttg tataagctgt caatagattt ggcacatagt
     6301 aataaaaccc atgatgatct aggcggcgat gtagcaaaag tatttcgatg tccacctgtg
     6361 ccgctagatc aatacaaact ttgacacgag aatccaatgc tgtcacccac cagcttaata
     6421 agcctcccat agaaagacca atcgttgcga gccgttgagt atctgtgtct tcccttgtct
     6481 ccaaataatc gatcaagctg atcacatcat aaatacgcat tccccataac gtttttcctg
     6541 ttaataaaaa ttgtttgaac aactcgcttt cagaaatacc tccgcgttct tcaaatcccc
     6601 aagcatcaat cgaccaaaca gcatatccca ttcctgtcag tacttctgca aaagatggag
     6661 aagctaagta aggagcacca tggatcaatt cacttttccc gactgaaaaa tctccgccgt
     6721 gagaatgcag gtaaaggacc ggcggaaacg gtccttcttt ttctggataa gcatagaggg
     6781 ctggtactgt ttccaattca tttaagtaca attgatagta ttcaagcata aaaccgtttt
     6841 gtttttctcg gtataatcgt tttccactag gggtatgtct gctagatgga gcacctaaaa
     6901 aatcattcaa ctgcataagc aaagttttta cgaacaacca gttcatccat attttctgat
     6961 tgatttgatt ggaatttctc aatactgatt tctttagtat tttctaatag aaatgctggt
     7021 ccctcgtgat tttcaatcga catctggtta aaggaaacct tacccgcaaa gccaatataa
     7081 aatccttgtc tgctcatttc ctctattcct gtcatcatag caggaacacc gggaactgca
     7141 tcttgggcca tcgagacatc gatattgtca aatgtgattt cttgtatgaa ttgttccgct
     7201 aatccataaa taaaacctgc agaagcatgg acattccttg ccgtgatatt cgaaaaatgg
     7261 atccttctga atgctggtgt ccgttcatct atcggataag cctttttttc ccatacatac
     7321 ggttctttcc ctcgcggacc acagaaataa taaagattca gtataaatgg acacatcaca
     7381 ttatccatga ctatattgga tactcgaata tcttcaacga tcccgcctcg acctcgtcta
     7441 gatttcaatc gtattcctcg atcagtttct tggaagatac aatttgaaat ggtaatatta
     7501 cgaatactac cactcatttc acttcctaat acgacaccgc catgtccatg gaccatcgtg
     7561 caatttgtga tcgtgatatt ttcgcaagcg atcctttcgt acgtatcttc agtccctgcc
     7621 ttgattgcaa tacagtcatc tcccacgtca atatggcaat tactaatacg cacatttttg
     7681 catgattctg gatcaattcc gtctgtattt ggtgaatccg ctgggttcaa gatcgtcaag
     7741 ttatcaaacg ttgcattact gcaaagaatc gggttgatcg tccaacttgg tgactggatc
     7801 aatttaatat ctttcacagt aattcgatga cagttatgga aactcatcaa tttaggacga
     7861 ggataagcga gattatctgg ttcatttcta aaagtatgcc accatttttt tccatttcca
     7921 tctaatgtac cgaaaccagt gacagagatg ttctctacat tttgagcata gatacaggaa
     7981 gcataaactt ttcgatggac tccttcccaa cgtgaatgaa caacaggata atcttttgga
     8041 tcgtcagaga atttcagaac cgctcctgcg cttaaatgaa gttcgacatt gctttttaaa
     8101 aataaagctc ctgttagaaa ttcacctgct ggaacaacta cggtcccgcc tccgtcactt
     8161 gctgcagcat caatggcttg ttgtattgct tcagtattca attcagcaat tgatgcgcca
     8221 aatgtcaaaa tatcatacat atttataatg cctccacttc aatcaatgct tgcaagaatg
     8281 caccaacgcc tttttgatca ttacaaataa ttggttcact gatgtagtaa gcatatgaac
     8341 catctcgctg atctgctccg cctaaccctg ctacttgaca gtttttattc aagttcagcc
     8401 agccttcttt tgtttccaga acaaattcat caagtaatcc ttgatgtgat ttcttcactt
     8461 gtggaagcca ttcttctttg ttcagcagtc ccaaacgtat tcctttagcc attgcgtaaa
     8521 caaacatact actgccggaa gcttccagat aatttccttt ccgttttgtt tctgttgtta
     8581 cttgatacca tgtcccgctg ttttcttctt gatatggaag taatgcagcc agtgtctttt
     8641 ctagaatttc tttcagcaaa gaacaatctg tttcttcatg aagtaattca taggtatctg
     8701 ctgcagccat caaataccag ccaatcgatc ggctccaaaa atgatgggat agacctgttt
     8761 ttggatcagc ccatggttgg accttctttt catcccatgc atgaatcagc aaatttgttt
     8821 ttggatcgaa tagatgttta taactgattt caaactgtct caaaacatct tggtaatctt
     8881 tttcttcact aaaattctgg ataaattcag cataaaaagg agaacccata tataagccat
     8941 ccaaccaaat ttgataagga taaatttctt tatgccaaaa agctccttcg gatgtgcgtg
     9001 gatggtttgc caactgctgg gaaaggagtt ctgctgcttt tttatatttt tcttcgcctg
     9061 tttctttgta caataaaaag aaaagctttc ctgtatttac atggtcgata ttgtattctt
     9121 caacgctgta gccacggatc gttccatctt cttgcacgaa atagtcgata ttgtctttta
     9181 tgaaatcaaa ataaattttt tcttgggttc gttcccaaag taattggaac cctttaagta
     9241 cgacgccata gtcataagac cattttcctt tataccactt ttcttcatat aagcgaggtg
     9301 ttctttccat gatacttttt gctaatttct ttgaccagtt ttccattatt tgctctcctt
     9361 taaaaattaa aataggattt gggacaaata ccaagaagta tttgtcccaa tcgtctatat
     9421 tactgtgccg ctgctttgta tgcttcctcg tactcttgtg tgattcgatc gcctccttgt
     9481 gctttccagt cttctacagc ttttttgaat ccatcttcgt caagctgtcc catgatgtat
     9541 tgcaccgtag catcaatgac gattttttca atttctgatc ctacttcatt gttcgtcggt
     9601 gaatccaatg gcacagaagg atccattaca gcaaattctt cattttcttt gatcaattga
     9661 ttagagaatt ctttttcagg gtctgtatct ttgaaagaga acgtcacttc actaggacga
     9721 gagcttgata acggttgcac ctcttgttgc caaagatcca tatcgataaa ttcgatagaa
     9781 ccatcatcat tcaattcata atgcgttcct tcgataccgc cagtcatcaa tttatacaca
     9841 tcttcttcta aaagatcatc aacaaattga agtaatttct tcaattgtgc ttcgtctttt
     9901 acttgagatt tgggaaatgc tagcaagccg ccaataccat ttccttctga ccaaacatga
     9961 taaccgccgc cttcaccatc tgagatcttg tttacaggca ctaattccat atcatcttga
    10021 ataccttgac ccatcgtctt cagattcttg atatccacca ttccagtgta gattccagtt
    10081 cttccttgtg caaattgttc ttgctgatca gtcttttggg taactgcaaa gtcttgtgcc
    10141 aaatagccat tctcaaatag atctcttgaa aaattcaaag ctttcatata ctcttctgta
    10201 tcaaattctg gagtgaattt gccattatcg tctacagccc agccatttgg tgctccaaaa
    10261 taagaagtga aaagtttaaa gctggtataa cgaatatcac taggcgcacg atcgccaaaa
    10321 ccaactgtgt cgtccacacc atttccatca ggatcgtttt ctgtaaattg tttagctact
    10381 tcataaagct catccatggt agttggaaca tctagtccta gattatccaa ccagtctttt
    10441 cgaatgacta agccagcacg agcgtagtct ttttgtatcg gtacgccgta caaggcaccg
    10501 tcgatactcg cagcttcgat tctatcttca gaaatttgac ttaaattttt atagtctttt
    10561 aaatattctg atacatccca gaatacgcct gattttaacg cattgcggac agaagagttt
    10621 ttcaacatcg caatttgaag tgtaacgata tctcctagtt catcagaagc caatgcagtt
    10681 gttaatcttt cttcttttga agcatcagga atccagttga atgtcagttc cgtatcggtt
    10741 ttttcttcga tcaagttcac aatcgtgtct gtaggcggtg aagcatgatg caaaatattt
    10801 aaccaagtga ttttagcttt tccatcttct gtctctgaag aactgttact tgaactgctg
    10861 ccgcatgcgg caagtgctag aattcctgca aaactaagac ctaattttac gagcgttgct
    10921 tttttcataa attttctcct tcactgtttt aataatattt ttgttttctt ctgttttttc
    10981 tgtatatgag ttcttccatt ccaaatggca aaatatagaa tttgctgtcc acgtgactgc
    11041 ggaaaagaag agactgccac cgataaaggt taaaacacct ggcattgttt ggatgatcat
    11101 cagataaatc cctaccatac ttaatatcaa cactaatgag taatgaagat gtgagaatcc
    11161 gaataaaaat gccattttca ttttgaaaaa atatcctttc caatcataat gagccatgat
    11221 tgggaaaata tagagtgctg agagcacata tatgaacgca gcaatataca aagcgatcct
    11281 aagataccaa ttgtgtatga aagaaagatc cacaagtaaa acaatcccga tggcgccata
    11341 tatccagctc atcagcaagg tttcacgata gttttcttta aaatatgtga agtacgtttt
    11401 cgtcaaagga aacgtttctt ttgagcgcag ccattttcta atcactgcaa ccatcgcata
    11461 cgatgcagga ccgaaagtaa aaactcccaa acctgcgatg actaaaccgc cccacagaaa
    11521 attcaaataa accaactgta aaatacgatt tagccttccg tttattttat ctaatgactg
    11581 cagcatatct ttccctctct ttctatctta gtaaacgccg tcttctccta gctttttggc
    11641 aatcttatta gctaaaatta ccataaacaa tcctacgacc cctttaaaca atcccattgc
    11701 tgtactgaaa cttaactgtc cattcttcaa tccagctgta taaatatagg tgtcgaaaat
    11761 ttctgcaacg cttcgattca atgaattcaa taacaagtac atatgctcga accctaagtc
    11821 taatgtctga ccgattttta gaatcagcaa ggtaatgatt actggacgaa ttgccggcaa
    11881 tgtaacatgc catgttttgc gtaagcgtcc tgctccatcc atttctgcag cttcatacaa
    11941 ctgtgtatcg actgcagtta tcgctgaaag gtagatgatc gtagaccagc ccaactcttt
    12001 ccagatcact tgtccgatat atactgtgcg tgtccattca ggggatgtta gaaaactgat
    12061 tttttcaaat cccattgttg caaggaattc attcaatacc cctccgtcaa cattcaaaaa
    12121 tacataagta atcgaaacaa tgattaccca agacatgaag tgagggatgt aaatgatcgt
    12181 ttgaatcccg tttttcaatc gtttgttcgt tacttcattt aatagtaaag acaaaatgat
    12241 tggcatcgga aaaaacacga agatatttaa tccgaacaag atcaacgtat ttcttaataa
    12301 tgtaaagaat gtcggttctg taaacaaacg aatgaaatgt ttgaaaccga cccaaggact
    12361 tcccattatt cctaaaaatg gttgataatc ttgaaaagca ataacgagtc cccccatagg
    12421 aagataccta aaaatcaaga aataaaggac tcccggcaga atcatcacat ataaaaattt
    12481 attcgttttg attcctgcaa ttattttttt tcgtcttctg agcttttctt cttttttcga
    12541 tagcgtcagt tgaaatggtt cttgtttctt tacactttcc ataagcccct ccttagtttt
    12601 gtgaatgttt gctgcttatg acttcatgat agataactat ctgaattacg tatataatca
    12661 tttttaccgt aagtaaaaac gcttacatag caatgatttt tgtgctatac aaaacgataa
    12721 agtgtatttt ttcacaaata aactacgcat agcaaacctt ctattttttt ctatctgaca
    12781 cttagcatag gaaatgatta tatacgtaat ctttcaccga tgataaattg aacatataga
    12841 aacagaaaca tttaatttat tttgaggagg agcctatcta tatgaaaaag aaagtctcta
    12901 taggagaaag aagttttaca cttttcaaca atacgtttct aattttgcta gctttgatct
    12961 gtatcgttcc ttttttaaat atcatcgcta catcttttgc ttctacacaa gaagtcgtcg
    13021 cgaaaaagtt tatccttttt cctactacct tttctttaga cgcgtatcgc tacattcttt
    13081 ctacgccgac catttttaga gcacttgctg tttcgattgg tgttacaggt gtcggaacga
    13141 tcgtcagtat gtgtgcaact tcactgatgg cctacggtct ttccagaaag tatttgtttg
    13201 gtcgaggttt cgtcaacttt cttgttgtat tttctatgtt gttcagcggg gggatgattc
    13261 caacgttctt agttgttcgt tctctagggc tggtcaattc ttactgggcc atgatcctgc
    13321 ctgtggctgt taatgcaatg aatatgatca ttatgagaaa ctttttccaa gccttgccgg
    13381 atagtttgga agaatcagca aaaatggatg gttgcacaga ttttggtgta tttttcaaaa
    13441 ttatgctgcc attagcgtta ccgtctattg ctactatctc gcttttttat gctgttactt
    13501 actggaacac ttatatgact gctattttgt atatcaacga ttcatctaaa tggccgatac
    13561 agattctttt acgccaaatc gtcattgtat caagtgggat gcaagcagaa agtagtgccg
    13621 ttgatgtgat tccaccagca caaacagtta aaatggctgt tatcgttatt gcaacagtgc
    13681 ctatgctgat tgtttatcct tttgttcaga aatattttgt caaaggagca ctcgtaggtt
    13741 ctgtcaaagg gtgattatgt actgaacgca aaatggtagt tgtgtcagaa gtggtcagct
    13801 tcaagaaata agaaggcatt tacgaaaatt gcttcttaaa tttttgtgaa atttcggctt
    13861 atttttcgaa gaatctactc ctggaacacc gtgtatcaga ggttgtgaaa gagacgttca
    13921 gccttgagcg atgataaaaa ctctcacaaa cattgcgacg agtaacccac aggagcaata
    13981 agaagcgatt ttgaaaaata gcttcccata tttcacaaaa ttatgcctta ttgtcgatag
    14041 cctgtctctt gaacgccatt tattcgtaaa aaagaactgt gacaagactt ttgtcacagt
    14101 tctttttgcg tgcttagcct tgaaatttaa ctatccataa atacaaatac tagggttatt
    14161 ctaacttctt ttattgcttg aatcagtcca caagaaaaac agcctcttga ttcacaaata
    14221 tttttttcga tattctcccg gcgtaatcga ttccttcttt ttaaagaaac gaataaaatt
    14281 ttgcggattt cgatattgca atctttctgc aatctctttt acggagaggt tagtctcttt
    14341 caaccatttt tttgctacct ctaaacgatg attttgaaca aaatcaccaa atttttctcc
    14401 tgtttccttt ttaaatacat tacttaaata gttagggtta taatgcaacc tatcagcgat
    14461 cacatctaaa gacagatcac gatcatattc tgtgtagacg atatgcataa tattctccga
    14521 caaattttta aattcatgat cagtgattgc ttgagagcat tctgtgattg gcagaactaa
    14581 ttgttctaaa acaagttttt ctagttcttt gggatgataa ttgttcagtg cttttacata
    14641 aatttgtttc atgttatcaa agttttttga atctactccc aataactgtc ctaattgaac
    14701 gagttcattg atcaagcgga ccgaaactac ttcccgactc aaaggatttt tattttgtgc
    14761 aaacaatgca tcaatcaatt catgtactaa attcttcact tcctgatttc cagaacgaat
    14821 ggcttcaaac aatcgatttt gttcttctat cggatatcta ataagtgttt tttcatgagc
    14881 agcaggaacg atttcctgat aaaaaataat tgaacttgga ccagtattta cacgataata
    14941 cagggcttct tttgcccgat caaccgattg cttgctttca ttcagcgttt cgaaccggtt
    15001 actgatgcct atactgatag ttaaattcaa gtattctttt acacgttttt gaatgagtcg
    15061 gcaatactcc atgacttttt tgcttgcttc tgcatcatct ttatccattc gataaatagt
    15121 ggcttgcatt tcttcattca aaacgatcgg cagcattcga ttttcctttg gcacaatttc
    15181 cgtgatcatg ttgttgatcg ctaaaagtag caaatcacgc tccccaccgt gactatcttc
    15241 taacagatcg atttgaacca aagctgtata atagcagtca ttttgagaac tataaccaaa
    15301 ttgctgcaac cgctgatcta gttctcgcct ttctactcga tttctaaata aactaagtac
    15361 aaatagcgtt tccagctgtg gcttttgggt aactaagtgt gcacttaacg cttctttttc
    15421 tcctatgatc cgactgactg attgagcgac caaatctagt tctttcccac tgaatccttt
    15481 tgatttaaga ttcagacgtt cctgtatttg agaaatcgga cgggcaaatc tgtccgaaaa
    15541 taagtaggaa agaagaccaa tgagtcctac cagtgttaaa gacactgcaa ctagcccgat
    15601 tctcagattg cggatcattg cgccaattgc actagagtca acttctaatc tgtaaatcca
    15661 attattataa tctgatttca gtactatata cgttttatct ttttctctta tcactttcag
    15721 ctgattgtaa tctagatcca cgtccgttaa tacagttggt tgctctttat tttttactgt
    15781 ctgagtagta aaaaggttgc caacttcgtt ttcaatagaa agtatgtctt ctccactttc
    15841 accaacaact tgttctattg tatgtttctc tatcgtagca attcccaatg catatttttc
    15901 tttttcaaac attggcaaag aaatcaccat ttcgattcct ttgtttttct tttgccaaaa
    15961 taaattattt ttgttattta tgtacttctg ttgatacttt tcaatttcct cttcggtcag
    16021 ttgactcaac gatccattga ttactcccca tttaccagcc aaactgacta aatcataatt
    16081 attatcatcc ataacaatcg tttctataaa atttagttct ttcctgattt cagaataagt
    16141 ggagtaatct gtatatgtca gcggatgctt gaatatgtcc gagaaatttt tggtcgaacc
    16201 atacgtagaa aacgaatatt caatcgttct aattttccat tcaacgttag ttttaacttg
    16261 cctgagcaaa ttctttttgt ctgttaaata actcgattcg atcgattcat aggtattctg
    16321 gtaaatgaat aaactaaatc ctatgacaag tacggctcct aataaaaaca tcggtaaaaa
    16381 tattttataa aatactgtac ttttcttcat tttcttcctc tcctcctttt aaaataaaag
    16441 gctttcattt ttctgtaaaa ttgatactca tctaattaaa tgagcattcc ccgctttcaa
    16501 gaatagcata attaaaaata aaaatccaag taatttttaa cgtgtgcaat cttttcatat
    16561 caaagatcta ttgacttatc gtagttatcg taatatgatg aatgcgttaa caaaaagtga
    16621 aaaagtaagg aaaatgatgg aatggtaacg atcaaagatg tcgctaaagc agctggcgtt
    16681 tctcatacga cagtttctcg tgctttgaat aacagcagtt taataaaaaa tgaaacaaaa
    16741 caaaaaatac gacaactagc taaggaaatg aattattcgc caaactatag tgcaaaaggt
    16801 ttagtgaatc aaaaaaaata tctaatcgga ctatttttct caagtttgca taaaggaact
    16861 tcatcaaatt tcctagcaga ttccattgca ggcatccatt cagtattaga cacaaattac
    16921 agtttatctg ttgaaagtat cgatgttgtt gatttaacag aaatcaacct gcaacgctac
    16981 gatggactga tcgttatgag tcaaagtgat gatgatcagc ctttcatcga ctatctcaaa
    17041 gaaaaagaat ttcctttcgt tgttgtcaat cgtcacatca atgatccagc ggttatcaac
    17101 gtcactgccg atgatgcggc cggtgttatg caggcagtag attacggtat tggatgcgga
    17161 catacgaaga tcgcttatat tggcggaaag aaaaattttc gttcgacaaa agaacgggaa
    17221 aaaggggtat tgaaaagtct aaaaaaagcg agcatccctg tcaatccagc ctatttttta
    17281 acaggagatt acagcttaga aagcggcctc caaaacatgg aatacctatt atcactttct
    17341 gacctgccta ctctggtttt ttgcggcaac gacgacatag cgattggtgc attgagagct
    17401 gtacatttag ctggcctccg tgttccccag aacatttctc tgatcgggtt cgatgattct
    17461 ccagtcgtct cctatctcaa tccgccgctt accaccgtac gtaagccttt gaaagaaatg
    17521 tgtcaagaag gaacaaagct tttactagat ttgatcgaag gcaggacgat cgaacaatct
    17581 aaaattgagc tctccacctt gttgatcctt cgtgagtcga tcaattactt gtaaactaca
    17641 agtaatttta tataaaaatg ttaacgtgtg cataacacag aaaggaagtt tatatgaaaa
    17701 aactgacaaa agaattagtg aaaaaaaacg aagcacctgt aaaagttctt cagtttggtg
    17761 aaggcaattt tatgcgtgga tttattgatt ggcagattca gcagctgaat aaccaacaat
    17821 tgtttaaagg taatgttgca atcattcaac ctttagaaca aggtcttgga aatatgatga
    17881 accagcaaga taatttatat acggtcattt tacaaggact cagcaatggc gaaatcgttg
    17941 atacttccga gattatcacc gtagtagaac aaatgatcaa tccttatgat gaatgggatg
    18001 cttatctatc tctagcagaa aatgacgact tggcctatat cgtctccaat acaacagagg
    18061 caggaatcca attcgtggag actgataatt taacagatac tcccccacag agcctcccgg
    18121 gcaaactgac agccttttta taccgacgtt tcaaattaaa caaagctggc tttaccatca
    18181 ttccttgcga actgattgac agaaacggcg aacgtctaaa agaaatcatt ttacgctacg
    18241 cagaaaaatg ggatttagaa cctgcttttg ttgagtggtt agaaaaagaa aatgttttct
    18301 gttgtagttt agttgatcgc atcgtcccag gttatcctcg cgaagatgca ggtgttttca
    18361 atcaacggca taactatgaa gatcaattga tggtcaaagc agaaccattc atgctttggg
    18421 tgatcgaagg gcctaaagaa ttggagaatc aactaccatt caaacaggct ggcttgaatg
    18481 tcatcatcac agatgatatg actccttatc gtgaacgtaa ggtgcatttg cttaatggac
    18541 cgcatacagc gatggtccct cttgctttgt tggcggaatt ggaaactgta gaagaagtaa
    18601 tgaaagatcc tttattctcc gcatatgttg atcagttatt caatctagaa ttgattccta
    18661 tgctttcttt acctaaagat gaattagcca tttatgctga ccaaatcaaa gaacgtttct
    18721 tgaatccttt tgcccatcac aaattagaag cgatttcatt aaatagtgtt tcaaagttca
    18781 gcacccgtct gcttccagtg ttcaagaagt atatcgaaga acaaaatcag gtaccaccgt
    18841 taatcactgt ttcactagct gcgctacttc ttatgtatcg aggcgatcaa gtaaagccac
    18901 atgatgatga aaagacgata agcgaattta ctgatgcttg gtctgataat ggaacagcta
    18961 ttcctcgatt attgcaaaac gctgcgcttt ggggagaaga tttgtctcaa ataccgaatg
    19021 tgacagatac agttcaagaa atagctgcaa agatcgatca aaaaggtgtc cggtcagtat
    19081 tacaagaaat gaaaggaacg atggtccatg cttgaaaccc tgcaaccaac aatgaaatta
    19141 aatcgacgag acagtgtggt cgtcgcatta acccctattc caaaacaaac acaactgacg
    19201 attgatcagc aaacgatcac cactcttgaa gatatcccgc aaggtcataa gattgcattg
    19261 ggtgatttga aagctggaga caacgtgatc aaatatggct atccaatcgg tcatgtgacg
    19321 acagacgtaa ctgccggtca atggctgcac actcataatg taaaaaccaa tctttctggt
    19381 gaattggatt atcgatatga aaaagatgtc catccaagtc attacctttt tgagaaccga
    19441 acatttcaag gttacttgcg aaaaaatgga aaagtaggga ttcgtaatga tttgttttta
    19501 gtacctaccg ttgggtgtgt gaatggcatt gctgaactga tcgtcaaaca gttcaaggaa
    19561 aaacatccag atctgggaca gtttgaccat attacgattt taaagcatcc ttatggctgt
    19621 tcgcagcttg gaaaagatca tcaaaatact cgagaaattt tagcggacgc agtaaatcat
    19681 cccaatgccg gaggagtttt agtatttgga ttaggttgtg agaacaatac agttcctgaa
    19741 ttcaaaaaaa ttttaggtga atatgatgaa gaacgagtga aatttctagt ggctcaagat
    19801 gtttacgatg agatcgaaca aggtgtggct ctattagaag agcttttaac agcggcggaa
    19861 aatgaccagc gcatatcagt cccgctctct aagttgaatg tcggtttgaa atgcggcggt
    19921 tctgatggtc tatcgggaat aactgctaat cctttacttg gtgcattttc tgactacttg
    19981 attgctcaag gaggcagcac gatcttaaca gaagtgccag aaatgtttgg cgcagaacaa
    20041 gtattgatgg cacgagcgga aaatgaagaa gtttttgact caatcgttga cttgatcaat
    20101 gacttcaaac agtatttctt gtcttacggg gaaccagttt acgaaaaccc atctcctggt
    20161 aataaagcag gtgggatcac tacattagaa gataagtctt taggctgcac gcaaaaatca
    20221 ggaagctctc ctgtcgttga tgtgctacaa tatggcgaaa aaatcagaaa accagggctt
    20281 agtttattgc aagctcccgg gaatgatctt gtagctgcct ctgctcttgc ttcatccgat
    20341 tgccagttgg tcttgtttac cacaggaaga gggactccgt ttggctcata tgtcccaacg
    20401 gtcaaggtat ctacgaatac aactcttttt gaacgcaaag gtcattggat ggattttaat
    20461 gcaggcgtct tattagaaca accaatggaa gtaatcttag aatcgtttgt tcaaaaaatc
    20521 atcgctgtag ctagtggaga agaaacgaaa aatgaacaaa atgacgttcg agaaattgcc
    20581 atttttaaaa acggagtaac actttgattt tcataaaaca gtataaaaag aaagaaggaa
    20641 gatttatgtt tttatctgat gattttttat taaaagacga ttgggcgaaa aagctttacc
    20701 attcctacgc aaaaaacatg cccattattg attatcactg tcacttagat ccaaaagaaa
    20761 tttacgaaaa caacaatttc agcaatctga cagaagcttg gttaggaggc gatcattaca
    20821 aatggcgctt gatgcgagct tgcggcgtgc ctgaagaaaa aatcacagga aatgcttctg
    20881 attttgaaaa atttttagct tggtgtcaaa ccgtaccaaa aattatcgga aatccgcttt
    20941 acagttggtc acatcttgaa ttgaagcgtt tcttcggtat tgacttgttg ataaatgaag
    21001 aaaatgcaga gaaaatttgg acacaagcaa atgaaatttt ggcagctcct gattttcgcc
    21061 gtcgggaaat cgcaaaaaaa tcgaatgtag aagtgatttg tacaacagat gatcccgcag
    21121 acgacttgca ctatcatcag ttgcttgctc atgaagaacc agatttgaag gttctacctt
    21181 cattccgtcc agataaagca ctcaatattg aaaaagaagg atttaatgca tgggtcacac
    21241 tactggaaga agcagctgac caaacgatca ccacctatca agaactgata gaagtcttag
    21301 ggcagcggat agaatacttc catcaacatg gatgtcgtgt ttccgaccac gctttagaca
    21361 tcttacgcta taaagaagcg gatgaaacag tattagaaga gatttttcaa aaagcacgag
    21421 ctaacaaact attatcttca gatgaaatcg atgcttatcg tacagaaaca ctgatacatt
    21481 tgacccactt ctatcattct cataattgga cgatgcagct gcacatccat gcttatcgta
    21541 actgtaatac acaaatgttg gaaaaattag ggcccgatac cggctatgat ggaatgaatg
    21601 atctttcttt gactattcct ctgcaaaaat tattgaaccg tgcagaagaa acggatcagt
    21661 tgccaaagac gatcctttat tcattgaatc cgaatgacta tcctgcgatt ttagctttga
    21721 tgggctgttt ccaaaaagaa cagaatggaa aactgcagtt aggttctgga tggtggtaca
    21781 acgatactcg agctggtatg cgcgatcaat tgacacaatt tgctgatggt agtgctttgg
    21841 ggaattttgt cggcatgctg acagattcaa gaagtttcct gtcctacact agacatgaat
    21901 atttccgtcg tgttctttgt gaatttttag gcgaaatggt catgcgagga gaagcacctg
    21961 aagatgaaca acttttagga agtctagttc aagcgattag ttatacaaat gctaaaaatt
    22021 actttggctt ttttgcctaa ttgattaata gtaacagtaa cgaatcaaaa acaggatagt
    22081 gaaataacgg aatccgttta tccacgtttt agagaagaag acggaccaaa tttgctccgt
    22141 cttcttctct tgctgttctg cttcattgcc tataaacctt tatgaaagaa gagataacat
    22201 gcctactatc catattgccg gtgattctac tgcggcagaa aaagatgaaa acaagcgtcc
    22261 cgagacaggt tggggagaaa aaatctctgc ttatttttca cccgagatcc agatcaataa
    22321 ccaagcaaag aatggacgca gcaccaaatc atttatccag gaaggaaggt tagcccgtct
    22381 agcttcacag tttcagccga atgatttctt atttgtacaa tttggacata acgaccagaa
    22441 aatccaagaa gaaagaggta cgcttccctt cggtagctat ttaaaaaatc tgcagatatt
    22501 ttttgaatgc gcaaaaaaag cgaacgtcca aatggtctta ctgacatctg tgacgcgacg
    22561 agattactta gaaaacggaa cattgaatcc agatatactt ggagactatc caaaagcgat
    22621 gcgtgcattt gctgagaaac accatattcc tcttttagat gtgttctcaa gatcacaaga
    22681 attgttccag accttctcaa aagaagagac aaaaaaattc tacctgcatc ttatacctga
    22741 tacttgtaaa aattacccag aaggactaaa agacaatacc cactttagtc cagaaggcgc
    22801 tgataaagtt gcccgaataa ttgttgaatt gataaaagaa tcccgtctgc ctcttgctaa
    22861 ttatttacaa aaaggagttt gagatgtttc cattaaattt aggcatccgt gcccacgatt
    22921 tagatgttcg aaatcgtgaa gaaatcgttc aaaaagtatc acagcttcac ttgtcccata
    22981 tccaatttgc accacaaaaa gcatttcctg aacaacttaa attaactgac atgacacttg
    23041 ggagtgctgc ctattttggt gaatacttcc aaaaaaacaa tatcgaactt tccattttag
    23101 gatgctatat caacctatcc agcaaaaatc aagaagttcg tgcacaagca ttggcaactt
    23161 tcaaacagca aattgctctg tgctctgcct atcaagccaa gatgattgct acggaaaccg
    23221 gtagtgtctc tgtcggttac acaaaagaaa attttactga agaagcttat cagatcgcca
    23281 gaaattctat cattgaatta gtcgctcatg cagaaaattt tggcgtaact gttgctgttg
    23341 aagcagggat caatcatcca atctataact atcagttagc taaacgttta atcgatgaag
    23401 tagcttcacc aaatttaaaa atcattttag attgtgcgaa tttgatgcat aaagaaaatc
    23461 atgctgatca ggaaaaagtg atccgaggtg ccttggacaa tttacatggt tatattacag
    23521 cgcttcatct caaagactat atcatggaaa atggaaagat ccgcatggta cctgttggca
    23581 agggctgttt agatttccgc ccggtactgc attatatcaa atatgagcgt cctctaatgt
    23641 acgccactct tgaagctact cctgagattt atgttcctga agcaatagaa cacttgcagt
    23701 ttatttacca agattgttaa ggagaagatt atggaggaac gaatacagaa aagttatcaa
    23761 aaatatcatg caaatgcttt tcagacattg ctgcatcttt atcagggaaa ttattggcgt
    23821 tttgccatgt caggactctt ttttattatc aaacatcttc catcttggtt aatgccgatc
    23881 gctattgcca atgtaatcaa tgcagcttca tcgaaagatt cttctcagtt gcaggccatt
    23941 tatttcaacg cttttttgat ggcttgtctg attggtcaaa atatattgac gaattatttt
    24001 catgtaaaat accatagcac ttcgatccgt caagtagaag ctggactaag agcgaccctt
    24061 atcaagaaaa tccaagaact atctattcct tatcaaaaag aattgcaatc tggacgaatc
    24121 cagtcaaaga tcatccgtga tgtagaagct atccaaacgt tgtcctctca agtatttgtc
    24181 agcggattaa atatcgctgt caatttattc gtagctttag gtatcacgct ttataaaagt
    24241 cctgttgttt tcatgttttt cttattgact gttcctctcg ccgcattcgt tactctcttt
    24301 ttccgtaaaa ggatcagaca gtccaactca gacttcaggc aagaaatgga agagacttct
    24361 gcaaaagtga tcgaagtgat cgaattagca ccgattgctc gagctcatgg gttagaaaaa
    24421 cacgaaacaa ctcgcttaaa taagcatttt actcaaatct atgaaaaagg attccactta
    24481 gatatcctgc aatctgtttt cggatcagtc agctgggcta gttttcagat atttcagctg
    24541 atttgtttga ttttcactgg gatattagct ttacaagggc gcatccaacc cggcgatgtc
    24601 gtgatgtatc agacgtattt cacgacgatc gtcaattccg tttctggaat gatcacgctg
    24661 attcctacga tttcaaaagg catggaatct atcacttcta ttggcgaact gttgattgct
    24721 ggcgatgtag aaacttatca cggtaagaaa aaaatccaac atttggatgg aaattttcgt
    24781 tttgaggatg tttcttttct ttatccggac tcactcaaac cgattatcga tcatttttct
    24841 ctagaggtga aagctggaga aacaattgct tttgttgggc catctggctc tggaaaatct
    24901 acactcctta acttattgat tggatttatc cagccgactt caggaaaaat ttttctagat
    24961 gatcagcctt tggatgaact ggatatgcga agtgtgcgaa attatttagc cgttgttcct
    25021 caaaccactt tgttgttctc cgcttcgatc aaagagaaca tcacttatgg cttgaaaaac
    25081 gtatcaaaag aacgtttaga tgaagtcatc gaagctgcgc aactttcttc actgattgac
    25141 cagcttcctg atggtctgga taccttagta ggcgaacatg gcaacaagct gtctggtggc
    25201 caaaagcaac gtatctccat tgcacgagcg ttgatacgtc agccaaaaat cattttgcta
    25261 gatgaagcaa cctcagcttt agataaccag tcagagaaaa aaatccagca agccctgaat
    25321 tacctgacag aaacacccac tacttttatt gtggctcatc ggctttcaac gatcaaagag
    25381 gcagataaaa tcgttgtgat cgatgaagga aaaatcgttg aaataggtac ttacgaaaaa
    25441 ctgttagctc aaaaaggcta tttctaccgc ttgaaaaatg aatgacaaaa aataggagct
    25501 atgacaagac ttttgtcgta gctcctattt ctgaataaac ggtgttctc
//
LOCUS       DACEUZ010000027        25516 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.027, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000027 DACEUZ010000000
VERSION     DACEUZ010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25516
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.027"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(136..843)
                     /gene="deoD"
                     /locus_tag="IXG44_000959"
     CDS             complement(136..843)
                     /gene="deoD"
                     /locus_tag="IXG44_000959"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAQ1657692.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(860..1678)
                     /locus_tag="IXG44_000960"
     CDS             complement(860..1678)
                     /locus_tag="IXG44_000960"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAQ1657693.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            complement(1702..2868)
                     /gene="deoB"
                     /locus_tag="IXG44_000961"
     CDS             complement(1702..2868)
                     /gene="deoB"
                     /locus_tag="IXG44_000961"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAQ1657694.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            complement(2967..3923)
                     /locus_tag="IXG44_000962"
     CDS             complement(2967..3923)
                     /locus_tag="IXG44_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1657695.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            complement(3923..5056)
                     /locus_tag="IXG44_000963"
     CDS             complement(3923..5056)
                     /locus_tag="IXG44_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1657696.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            complement(5049..6611)
                     /locus_tag="IXG44_000964"
     CDS             complement(5049..6611)
                     /locus_tag="IXG44_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1657697.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            complement(6823..7905)
                     /locus_tag="IXG44_000965"
     CDS             complement(6823..7905)
                     /locus_tag="IXG44_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ1657698.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            complement(7971..8369)
                     /locus_tag="IXG44_000966"
     CDS             complement(7971..8369)
                     /locus_tag="IXG44_000966"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HAQ1657699.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            complement(8390..9052)
                     /gene="deoC"
                     /locus_tag="IXG44_000967"
     CDS             complement(8390..9052)
                     /gene="deoC"
                     /locus_tag="IXG44_000967"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HAQ1657700.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            complement(9098..10399)
                     /locus_tag="IXG44_000968"
     CDS             complement(9098..10399)
                     /locus_tag="IXG44_000968"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HAQ1657701.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            complement(10540..11556)
                     /locus_tag="IXG44_000969"
     CDS             complement(10540..11556)
                     /locus_tag="IXG44_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1657702.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            complement(11864..12469)
                     /locus_tag="IXG44_000970"
     CDS             complement(11864..12469)
                     /locus_tag="IXG44_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ1657703.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            12665..13588
                     /locus_tag="IXG44_000971"
     CDS             12665..13588
                     /locus_tag="IXG44_000971"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HAQ1657704.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            13647..15212
                     /gene="guaA"
                     /locus_tag="IXG44_000972"
     CDS             13647..15212
                     /gene="guaA"
                     /locus_tag="IXG44_000972"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAQ1657705.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(15703..17334)
                     /locus_tag="IXG44_000973"
     CDS             complement(15703..17334)
                     /locus_tag="IXG44_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-1,4-beta-galactosidase"
                     /protein_id="HAQ1657706.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(17348..19021)
                     /locus_tag="IXG44_000974"
     CDS             complement(17348..19021)
                     /locus_tag="IXG44_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1657707.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            complement(19034..19351)
                     /locus_tag="IXG44_000975"
     CDS             complement(19034..19351)
                     /locus_tag="IXG44_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAQ1657708.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            complement(19376..20776)
                     /gene="lacG"
                     /locus_tag="IXG44_000976"
     CDS             complement(19376..20776)
                     /gene="lacG"
                     /locus_tag="IXG44_000976"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAQ1657709.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            21013..21798
                     /locus_tag="IXG44_000977"
     CDS             21013..21798
                     /locus_tag="IXG44_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ1657710.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            21801..22493
                     /locus_tag="IXG44_000978"
     CDS             21801..22493
                     /locus_tag="IXG44_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ1657711.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(22767..23138)
                     /locus_tag="IXG44_000979"
     CDS             complement(22767..23138)
                     /locus_tag="IXG44_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HAQ1657712.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(23538..23768)
                     /locus_tag="IXG44_000980"
     CDS             complement(23538..23768)
                     /locus_tag="IXG44_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1657713.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            complement(23862..24200)
                     /locus_tag="IXG44_000981"
     CDS             complement(23862..24200)
                     /locus_tag="IXG44_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1657714.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            complement(25128..25244)
                     /locus_tag="IXG44_000982"
     CDS             complement(25128..25244)
                     /locus_tag="IXG44_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAQ1657715.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
BASE COUNT         7435 a         5603 c         4500 g         7978 t
ORIGIN      
        1 ttcacaacct cttggtaaac ggtgttcaaa agcttacatt tgcaataata agtccagcca
       61 agtcaaaaac atgaggagct atttttgctt ggatgttctt attacttaat gcagggcagc
      121 tttttcattt ccttcttatt ctgctatcgc tgtttccaat cctacttcga tcatttcatt
      181 gaacgtagat tgacgttctg cagctgttgt ttcttcaccg gtgatcaaat ggtcgctaac
      241 ggtcatcaaa cttaatgtct gcacatggta tttcgcgcct aagtaataaa gagccgctgc
      301 ttccatttcc actcctaaaa cgccaagttc agctaattgg aacgtctctg tcaaatcatc
      361 tttgtagaaa gaatcttccg aaagaacatt tcctacatga accgtatagc ccttttcttt
      421 agctatctca tatgctttca gtaatagatt gaaatcactg attggaggaa aatgaaaggc
      481 ttggaaattt ttttcgatca tcgaagaact ggtcgctgca ccttgtgcta gtactaaatc
      541 acgaacatgg acgtcttttg acaatgcacc gcatgtacct actcgaatca atttctttac
      601 accatacgac tggatcaatt catgggcata gatggtggct gatggcattc ccattcctgt
      661 tccttgaaca gaaatacgtt ctcctttata tttgccggta aagcctaaca taccgcgtac
      721 ctgattgtaa caaactggat cttccaaaaa agtctccgca atatatttag ctcgcaaagg
      781 atctcccgga agtaaaatct tatcggcgat ctcgccttct ttcgcttcaa tatgcacact
      841 cattttattg ttcctcctat tacaataatg tcaatgtttc tttaaccaat gctttaaaag
      901 attgtttgac tctctgagtt gtttctacaa cttcttcatg gctcagattc gcttgcatac
      961 ctgctgctaa gttggtaata caagagattc ctaacacttt taatccgctg tgagctgcta
     1021 cgatgacttc tggaacagtc gacatcccta ctgcatcggc tcctattgtc cgagacattc
     1081 ggatctccgc aggcgtttca taagttggtc cagaatagcc catatatacg ccttctttaa
     1141 gatccaaacc tagtttttct gctgctgctt ttgctgttgc ttgatagtca ggcgtataag
     1201 cttgactcat atctggaaat ctagggccca ttgcatcttc attcggaccg atcaaaggat
     1261 tttcaccagt aaaattgata tgatcggtaa tcagcatcaa atctcctggt acaaatgatt
     1321 cgttgacacc accacatgca ttagtcacaa tgactgaatg ggcccctagt ccggccatca
     1381 cacgtactgg ataggtcact gtttgcatgg aatgaccttc atagtagtgg aatctccctt
     1441 gcatggctaa cactttcttg cctgccagag acccataaac tagctttcct gcatgcccaa
     1501 ctactgtcga aacaggaaag aacgggatct catcataaga aatcattaca gcatcttcga
     1561 tttcatctgc taattcacct aatcctgaac ctagaatcaa accaaaatca atttctttta
     1621 ctcctttatc aaggataaac tgggtcgttt cttgtaacat ctcgcttaac ttggtcattt
     1681 gctttctcct atttaaaaag attattttaa taatttcaag aagctttcgc cattttcagt
     1741 cgaagaaaca ctaaagttct cagcaatcgt tgctgaaata tctgaataat atccttgagg
     1801 caatgcccct tgttctttca tttttttact gtaaaccaat aacggtacat attctcgcgt
     1861 atgatcggta ccagtaaacg tcggatcgtt tccgtgatcg gctgtaatca ttagcaagtc
     1921 gtcttcttcc atcgcttcaa gcaattctgg aattcgtaag tcaaattctt caatcgcatg
     1981 cgcatagccg actacatccc gacgatgacc gtataatgca tcaaaatcta ctaaattggt
     2041 aaagctgatt cctgaaaaat cgtttttcat aactttcagc agctggtcaa cgccatccat
     2101 attgcttttc gtgcggacag attcagtgat tccttgtccg ttgaaaatat cgttgatctt
     2161 tccaacagca atcacgtcct ttcctgcttc tttcaaagaa tcaaggactg tgtgtccgaa
     2221 tggattcaac gcataatcat ggcgattgct tgttcgtgtg aagtctcctg gttcacccac
     2281 gtacggacgt gcaatgattc ggccaatcat atacggatca tcttttgtta tatcacgaac
     2341 gaattggcaa atccgatata attcttctaa aggaatgatt tcttcatgtg ccgcgatctg
     2401 aaggacagga tcggcagaag tatatacaat taaatcgcct gttttcattt ggtgttcacc
     2461 gtaatcgtca ataacagctg ttccgctata aggtttgtta catacgactt tacggccaga
     2521 aaattcttcg atttgtttga tcagatcatc agggaatcca ttaggaaaca cacgaaatgg
     2581 agttttgata ttcaatccca tgatttccca gtggcctgtc atcgtatctt tccctacaga
     2641 aacttcttct aatttcgtcg catagccttg atgatctttc actgattcca caccttcaag
     2701 aggacggatc gttcctaaac caagctgctc taaatgtgga acgttcaatc ctgcttcttt
     2761 cgcaatgtgt cctaatgtat cagaaccaac atcaccgaac ttatcggcat ctggtgcttc
     2821 tccaattcct actgaatcca tgacgatcag atgtacgcgt ttaaacatga ttattccttc
     2881 tttcttttta tttttattgc ctttaaaaca aaaagggcga gacgaaaaac acttcccgta
     2941 acccaccctt tttattcgct aaaagattat tttgatttga tatagttctt cccgtttgct
     3001 tttggaccag aagctttccc taagaaaaca accagcacaa tgattgtcag caaatatgga
     3061 gcaatttgca gcaatactgg cggaatcgaa gaaataactg gtaattgtgc gccaatgata
     3121 ctcaagcttt gagcaaaacc aaagaacaat gaggcaccca ttgcccctaa aggattccac
     3181 ttaccaaaga tcatagcagc catggagatg aacccttgtc caacgatcgt accagctgaa
     3241 aagttaccgg aaatcgactg agcaaaaaca gcaccgccca taccgcctaa caagccagaa
     3301 atcagtacgc ctgaataacg catagcatat acattgatac ctaatgtgtc ggcagcttga
     3361 ggattttcac caactgaacg gaggcgcaag ccgaatttcg tcttgaagat gatcacccaa
     3421 gctaggacag caatcaaaat agcacaataa gcaggtaaga atgtattttt aaagaaaatt
     3481 ggtccaatga ccggaatatc tgctaatcct gggaaagaaa aataccccag attctgcgtg
     3541 atgttatcag tttgaccttt accataccat gctttgatca agaaaacacc taaagcaggt
     3601 gccatcagat taattactgt cccgctgata atatggtcag cacgcaaact gactgttgct
     3661 actgcgtgaa tcagagaaaa taggatacct acgacgccac ccactaaaat agcgatccat
     3721 ggtgtcatag aaccaagtga tggactcatc gtcaagttga aaacgatgga actaaaagca
     3781 cccatgacca tgattccctc tagccctacg tttacgatcc cgcttcgttc agaaaaagtt
     3841 ccgcctagtg acgttagaat tagcggtgtt gaataaacca gtgtagaagt aaggatcaag
     3901 gcaattaatt cgatagtcga cattatagtt cgcccccttc atggttttga ttcgttggtt
     3961 tcgattctat tgtcgcaacg atctcttctt gttttttccc ttggaagact ttagccatga
     4021 cgaaacgaat caggaagttg atcccgacaa agaagataat caaagcaatc gatacattga
     4081 cgatctcaaa cggaacacct gctcctgtct gcatgccaag tccaccgatc ttcaaaatac
     4141 tgaacaagat cgctgataaa agaataccga ttgctgatcc agaaccaagc aaggaaacag
     4201 ccattccgtc aaatccaata gatagcgaag tcgtttgaac aaagaagttt tgatacgttc
     4261 ctaatccttc aaccacaccg ccaagccctg ccaaagcgcc agaaatgacc atcgacaaaa
     4321 cgatcgtacg tttactactc attcctgcat actcagaagc aaaaggattc aatccaacag
     4381 aacggatttc aaatcctaaa gtcgttttct tcatcagcag ccaaacgaaa ataagcgcga
     4441 tgattgctaa aaataatcct gagtttacgc gagagccgcc aaaagcttgt gttaaccatg
     4501 gtaaccgcag tgaggcattt tgactgatca tattggaaga gtccaaactt gagcgatagc
     4561 tttcaggcat cacttcatgt aaagcaaatg tgcttacgta taataagata tagttcatca
     4621 tgattgttac gatcacttca cttgttccaa aaaatgctcg aagtaaacct ggaacagccg
     4681 ctgccatcgc accagctaag gcacctaaga tcacgcataa cggaagcaaa acaacttttg
     4741 gcagatctgg aaacgcaagt gccacccaga cactgacgat ccacccgcaa agtgcttgtc
     4801 cagaaagccc aatattgaaa aacccagctg agttagctac agaaaaacct aatgctgtaa
     4861 aaatcaacgg cgttgcttgc cttaacgttt cacctatccc tctttgcgaa ccaagggctg
     4921 catctagcat agaagaataa ccttggatgg gctgaaaacc aaaagcagcc atcaatatcg
     4981 cgcctaagat caatcctaaa acaactgaaa gtacaggcac taataaatta cggaattttt
     5041 cgctgcgatc attcatgtgc gccagccccc ttttctaact cttttcgtgc ttcttctaag
     5101 gtatagcccg ccatcaatag acctaattca ttttcagatg tttctttcgg atcaacgata
     5161 ccgacaattt ctccttcatg gataacagca atacgatccg aaacatttaa aatttcttct
     5221 aattcgaaac tgatcagtaa cactgctttg tatttgtccc tttgttcgat cagacgttta
     5281 tgaataaatt cgatcgcacc tacatccagt ccacgagttg gatttgcaac aatcaacaga
     5341 tcaggatctc ggtcgacttc acgagcgata attgcttttt gctgatttcc gcctgataaa
     5401 gcttttgcgg gaacaagttc attcgttgtt cgaacatcat attcttcgat caattctctg
     5461 gcatgttcat tcattactga ataattcagc atgccgtttt tactgtaagg tttttgatga
     5521 taggtttgta gtgcgatatt ttcagataat gtcatatcta ggactaaccc gtatttatga
     5581 cggtcttctg gcacgtgtcc tacaccatgt tcagtgattt ttcggggttt tttattagtg
     5641 atgtcttccc cttttagctt gatatgtccg ctttctgcct ttcgcaaacc agtcaaagct
     5701 tggatcaact ccgactgccc gtttccatcg attccagcga taccaagtac ttcgccagca
     5761 cgaacctcta agttcaagtt tttcacggct tctaatccac gattttcttt caccactaga
     5821 ttttcaatag acaaaacgac ttcttgaggc ttcgcttctt ttttcatcgt tttgaatgaa
     5881 accgttcttc caaccatcat atcggctaat tgttgtgagg ttacgtcttt aacgttgact
     5941 gtaccgattc cttttccacg gcggataact gtacagcggt cagctactgc tttgatttca
     6001 tctaacttat gcgtaatcaa aatgatcgac ttgccttctt tgactaattc cttcatgatc
     6061 acgattaatt catcaatttc ttgaggggtc aatacagctg tcggctcatc aaaaatcagt
     6121 acatctgctc ctcggtaaag tgtttttaaa atttctaccc gttgttccat tccaactgaa
     6181 atatcacgaa cgtaagcatc tgggttgact gacaatccat actgttcaga aactttttgg
     6241 atctcttttc gtgctttttt atggtcaagc atccctgcgc gactaggctc gcttcccaac
     6301 acgatgtttt ctgttacagt aaaggcatca acaagcataa agtgctgatg gaccatccca
     6361 atccctaatt ggttggcttt ggtcggactt gtgatcttta cttctttccc acgcatcaaa
     6421 attttccctg atgtcggctc taataatccg gaaagcacgt tcatcaatgt agatttgcca
     6481 gcaccgtttt ctccaagcaa cgcatgaatt tctcctgcct ttacttgtaa attgatgttg
     6541 tcatttgcct tgaatgttcc aaattctttg gtaatattct gcatttctat gacaatattt
     6601 tcttgaacca cctttttttc acatcctaac gatttctatc tagggcaacg attgactaag
     6661 attcacgctt agtgagcgtt cttcttttga aacaaacatt acttcaggga gttgttccct
     6721 tatccaaact gtcacaaggc agcttggata aatgaacaat tcttgttcta atcaaaccat
     6781 agccttgtca aacatgtgtc taacaaggct atagaaatgg ttttagtttt cttcaggagt
     6841 ttctggaact tttacgtcac ctgaaatgat tttttctttt gcttcatcaa ctgctttttt
     6901 cgcttcgtca gaaagttgtc cttctgttag accaacaccg tcttctttca atccataaac
     6961 agtgtgttcg ccgccaggga atttaccgtc cgctgatttt tgtgccaaat cttctactac
     7021 tgttccaact gctttcagtg tagatgtcaa tgtgaagttt ttcttttcgc cgtttaatgt
     7081 gtattcgcct tcgtctgatt ggtcgcggtc aacaccgata acccaaactt ttttgtcgcc
     7141 tgattcgttc aatgattttg cttcttggaa tacaccgtta cctgtaccgc ctgaagcatg
     7201 gaagatgata tctgcgtttt gtgcatacat accttgagca attgaacgtc ctttatctgg
     7261 agcagagaag tcaccagcat attgattcaa tactttgatt tcttttccta attctttcgc
     7321 acctgcatca acaccggctt tgaatccagc atcaaaacga tcgattactt caccttttac
     7381 accgccgata aagccgacta cgtttgtttc agtcgtataa gctgctgcaa cacctgcaag
     7441 ataagaagct tcgttgtctt tgaaagttgc agatacaacg ttatctaatc catcgatgac
     7501 atcatcgata ataacaaagt tcgtacctgt attgtttgtt gcttgttctt ggatcgctgg
     7561 ttttagttta taaccaatac caaaaatcgt tttgaatcca gcattcaatg cttggtcaat
     7621 atttgggatg taatctgatt catttgaaga ttggaaatat tggaagccat catttcctcg
     7681 tgaaagtcct tgatctttcc cccatttttc tagtccttcc caagctgact ggttgaatga
     7741 acggtcgtct acaccacctg tgtctgtgat caatgctgca gttgttgtgt ctgatgagct
     7801 tttgccaccg gatgaatcag tagagccgtt gttgccgcat gcacctaaaa ataaccctgc
     7861 tgctagtgcg acagcaccta aaccaaataa ttttgctttt ttcatttctg taaagcctcc
     7921 taaaataatt gttttatttt tcaacagtca gctgactgaa tgaacatgag ttatagatct
     7981 ttttcagtaa atgcataagg cagcagtgca cgaacggtcg ttgccttcgt cacaccattg
     8041 tctcctacta gcgtaaccgg catatcaggg gaacagaatt ctgccattac ttgtcgacat
     8101 gcaccacaag gagagatagg atcgggggtg tgccccgcta cgactagatg cgtaaatgtt
     8161 ttttctcctt cagatactgc tttgaaaaaa gctgtgcgct cggcacaatt cgttaatcca
     8221 aatgatgcat tctcaatgtt gatgccttga taaatcttgc ctgattccgt tacgagacaa
     8281 gcgcctaccg gaaaatggga ataaggaaca taggctttat ctaacgcttc aatagctgtg
     8341 ttgatccatt cttgtttgag ttctgccatg agcttctcct ttgaaaaaat taatagcctt
     8401 caccagttga accagatacg attgctactc ctgcgcttgt tccgattctt gtagcaccag
     8461 cttcgatcat tgccattgct tcttcttcgt tatggatacc gccggaagct ttgacaccca
     8521 tttctggtcc tacagtttca cgcatcaaac ggacatcctc tacctttgct ccgcctgttg
     8581 agaaaccagt tgatgttttg acaaaatcag cgcctgcagc ttttgctatt tcgcaagctc
     8641 ggatgatttc ttctctatct aataacgatg tttcaataat aactttgaca agcgcacgat
     8701 cttttgctgc ttcaacaact gcttcgatat ctttttggac ttgttgatca tcctttgatt
     8761 tcaaagcacc gatattgatg accatatcga cttcatctgc accgttattg atggcatctg
     8821 ttgtttcaaa tgcttttgtc tcgggtgtgt ttgctcctag aggaaacccg attactgtac
     8881 atacggctac tggctcacct tgtaattgtt ctttcgctag cgcaacccat gctggattga
     8941 tgcagacaga ataaaaatgg tatttttttg cttcttcgat aatttgcatc acagcttctt
     9001 tagatgcgtc tgctttcaaa atagtatggt cgatcattcg gtttagttcc atttcttttc
     9061 cccttttctt taaaacggta aagtccgctt ttttccttta ttctgtgatg atgtcatgaa
     9121 tcaacgttgg cttcattgcc gattcaccaa tttcaatatt tttatataat aattcaatga
     9181 cagaagtgat ttcttcgtcg ttactataga tcgtcaatag tgactcacct tttgtcacag
     9241 tgtcaccaat ttttttatgc agtttcaatc caacagcgtg atcaatatct tcttctttgg
     9301 ttttacgccc agcacctagc atcattgcag caattcctaa ttcatttgcc acgatttttg
     9361 acacaactcc gctttgttta gcaggaagct ccattgtgta gcgtgcagtc aaaattcgtt
     9421 ctggatgttc cacgatcgct gggtctcccc cttgattttg gatcatttct ttgaattttg
     9481 ctaacgcttt acctgattcc agagcttctt gaagcaacgt tcttgcttca tcggttgtct
     9541 tagctttgcc agcaagaaca accatttggc ttcctaaggc gtagcacatt tctactagat
     9601 cttctggacc atttccttgt aatgtttcga ttgcctcgac aacttctaga ctatttccga
     9661 tagcttcgcc aagaggttct gacatatctg agataaccgc cactgtttct cggttagcca
     9721 gtttcccaat gctcgtcatg gtctttgcca gacgtcttgc atcttcgatg ttcttcatga
     9781 aagcaccgtc tccagtggtc acatccaaaa cgattgcatc tgctcctgcc gcgatttttt
     9841 tactcataat cgaactggca atcagcggaa gagaatcaac ggtcgctgtg acgtcacgca
     9901 aggcatacaa ttttttgtcc gccggtgcca agtttcctga ttgaccaatt accgctactt
     9961 tactttcatt aacaatgcgt acaaattctt catccgaaag ctcgatctga aatccgggaa
    10021 ttgcttctag cttgtctagt gtaccacctg tgtaacctag accgcgtcca gacattttag
    10081 ccactgtcac tccgacacta gctactaggg gggctaaaat caatgtggtc gtgtcaccaa
    10141 cgccacctgt cgaatgctta tctaccttta ttccttcaag agaactcaaa tcgatgacat
    10201 caccagaatt agcaatcgcc aatgttaagt tcgtgatttc ttcatttgtc atgtcttgat
    10261 aaaaaattgc catcaataat gcactgactt gataatctgg aatctttcca ttcgtatagt
    10321 tcgtgacgat gtactcgatt tcctctttcg acagctcgtt tccatccctc tttttttcaa
    10381 tcagatccac cattcgcatt gcctgatcct ccttgtcttc attcggtatt atacattatt
    10441 tttaggaaag gtaaaccact ttagtaaact ggtttactaa aaataaaaaa agcactaact
    10501 agcctttgta aatgcttaca taagcaatat accgccgagt tagccctttg tcaatacttt
    10561 tacgctctca cgaataatta attttgtttc gaacactttg ttcggaactt ttctttgagg
    10621 aaattcgatc gtatcgatca ggaaacgtgc agcataaaat ccaatatcaa aacttggctg
    10681 ctgcactgtc gtcaattgcg gaatcatgta ttctgacagc tttaatccat cgaatcctat
    10741 gactgagata tcttctggga tttttttgcc caattcatcg atcgcctgat agcatccgat
    10801 cgccatggca tcattcccgc aaaaaatagc cgtgatatct ttatgtttga gtaatttttt
    10861 tgatgcagta tatccacctt ggacagtcaa ctctccatgt tcaacaaatt tcccattaaa
    10921 aggcaggcca tgatctttca gcgcatgacg atatccgttg aaacgttctt ctaattgata
    10981 gtatccagtg gtttccttca gcatgccgat cttcgtatgg ccattttcaa taaacagcga
    11041 aacagcttga taggcgccct catattcctt tacgataagc cgtccgttat cccgacgatt
    11101 caatccgcga tcgattaaaa tgattggcat tttttgataa aaaccgctgt ctaatgcata
    11161 tttctcaggt aaaatatttg gtgtggccag caagattccg tccactgagc ggtgggaaag
    11221 ctcttgtaaa tacttgatct cattttcttg gctatgacga gaattgcata acatgatcac
    11281 atagcccaaa ggattcaaat agctctctac accttcgatc agtttggaga agaagaaatc
    11341 agtgacatca gggacgatca ttccgatggt ctttgaatgt cgattgatca catttgaagc
    11401 gaaataatcg ggcttataat gatgttcatt cacgatcgac aacacttttt ttcttgtctt
    11461 atcactaaat ctgcttcctt tgttattcaa aatctgtgag acagttgtta ctgatacgcc
    11521 tgccatttct gcgatatctc tgatggtcag cttcatccta ttggctccct tctatctaaa
    11581 ttccctatca gtgtaccaaa acccccaaga aaagaataga gaaaaaagga aagtatgttt
    11641 tttgataaaa acctccttta tctaccgact tggcagcctt ctatacaagt tctgtatctt
    11701 atttatttta tcgtaaaaaa caacgactct gttcctacta tttctttttg aagaaaaaat
    11761 agttctttgt ttttaaaaac ttctcaacaa aaaaagaact gatacgtacc cgtacatgca
    11821 cgcccagttc cactctttcg tcgctccact atttttcagc tagttattct ttcacacttt
    11881 tgatgatata atatccttta tcttttgtga cgatttctgc attaccaaat gtttcttcca
    11941 ttttcttttg cgcactcggc gctccttgtt tcttctggat cacaattgtc agtgtaccgc
    12001 ccttcttcaa ccgaggatat gcgcctgtca aaatttcatg tacaactttt tttccagcac
    12061 ggatcggcgg attgcttacg atagctgcat aggtttcttc atgaactgct tcatagatat
    12121 tcgattgatg gatatcaaca tgttcgattt ggttacgctt tgcgtttcct tgcgctagac
    12181 ttaccgctcg ttcattaatg tcgaccattt caacaaaccg ctgtgtcgca aatgccaaag
    12241 ctaacccaat cggtccatac ccgcatccca catctaagat ctttccttca tcaggtaact
    12301 cttcccaatc gaaagcatca atcaggacac gtgaaccata atccactgtt tcacgtgaaa
    12361 atacaccaga atctgttaca aattgaaagg ttctgccttt taactcaaaa gcccattgtt
    12421 caaaatcgtg cgccgtatct gggcgttcag aatagtaatg atttgtcatg tcttccatcc
    12481 ttctcatttc tttcatgaca acagtctatc cttttatgta tccgtgcgca agtcaagaat
    12541 aagactccgt ttctttctat tataaagatt atacaaaaag atcggcaatc tctcttaagt
    12601 ggtggggttt gcgtttattt tgtgttaaaa tagttccatt gatgggagaa cgcaggaggc
    12661 taatatgaat gatccaatga actactaccg tgtggcaaga gaagaatggc gagaatttta
    12721 tcgtaatggg caagcacctt tgacacaaga tgaactagat aatatcaaaa gtttgaatga
    12781 ccgcatttcc atgcaggatg ttcgagatat ctatgtacct cttgctcatc tgatttatct
    12841 ttatatgaaa gaatttgaat ctttgttctt gagtaaaggt ttatttcttc ataaatacgt
    12901 agaagtaccg ccttttatta tcgggatcgc cggaagtgta gccgttggaa aaagtacaac
    12961 agccagactg ctgcagacga ttttgtctcg taccttcaaa cgaagaaacg ttcagctgat
    13021 cacgactgac ggatttcttt atccaaatcg tatattaaaa gagcgtggaa tcatggaccg
    13081 taaaggattt cctgaaagtt atgatatgga gatgctgatc gatttcttga accgtgtaaa
    13141 atctggtcag gaagagataa aagtacctgt ttactcacat gatgtgtatg atatcattcc
    13201 agacaagtac gaattgatcc agcagcctga tatcttgatc gttgagggga tcaatacgct
    13261 tcaattacct gcgaatcagc aaatctacgt cagcgacttt tttgattttt ctgtctatgt
    13321 cgatgccgat cccgtgctga tcgaaaaatg gtatttggat cgttttggtg cgttactgga
    13381 tacggctttc caaaatccag agaattatta ttatcgtttt gctatcggag atcgtgaaga
    13441 agcctttaaa atggcaaaag atgtatggaa aaatgtgaat ttgaaaaatt tacaggaata
    13501 tatcttgcct acacgaggaa gagccgatat aattctccat aaaactgaaa atcatatcat
    13561 cgattcgatt ttcatgcgaa aatattagca ttttaaaata gtttgactat tttgagataa
    13621 aatttaaagg gataggggta ataaatttga caaacgttgc cgatatgacg aacgttgaaa
    13681 agattatcgt attagactac ggtagtcagt ataaccaatt gatcacaaga cgtattcgtg
    13741 atcttggagt cttttccgaa ttattgagcc accgcattac tgcagaagaa gtaaaagcga
    13801 tgaatccaaa gggaatcatt ctttctggcg gaccaaacag tgtatatgat gagaatgcat
    13861 ttgggatcga tcctgagatc ttcaatttag gtattcctgt attagggatt tgctatggta
    13921 tgcaattgat cacgtacaaa cttggcggtt ccgtagagcc tgctaaaaac cgcgaatatg
    13981 ggaaagcctt gttagaagta acaagcgatc aagctgtctt gtttaaagaa acaccaaaac
    14041 aacaaacagt atggatgagc catggtgact tggtaactaa agttcctgaa ggctttgaaa
    14101 cagtcggaac aagcaaagat tgtccgattg cttctatcca agacacaaca cgtaattttt
    14161 atggtatcca attccatgca gaagtacgtc attccgaata cggaatcgaa ttgctgcgcc
    14221 actttgcctt tgatgtttgt cactgtgaag gtaactggac aatggataac tttatcgata
    14281 tgcaagtaga aaaaatccgt gaacaagttg gtgacaaaaa agtcttgctt ggtctttcag
    14341 gtggcgttga ttctagtgtc gttggggttc ttttacaaaa agcgatcggt gaccaattga
    14401 catctatctt tgttgaccac ggtctgctac gcaaaggcga agcggaacaa gtaatggaaa
    14461 gtctaggcgg aaaattcggc ttgaacatca tcaaagtcaa tgcaagagat cgcttcttag
    14521 acaagttgaa aggtgtatct gacccagaac aaaaacgtaa aatcattggg aatgaattcg
    14581 tatatgtttt tgatgacgaa gcaacaaaac ttgctggtga agaaggcgtc tctttcctag
    14641 cacaaggaac gttgtatact gacgttatcg aatctggtac agaaacagca cagacgatca
    14701 aatctcacca taatgtaggt ggactgccag aagatatgca atttgaattg atcgaaccat
    14761 taaacacatt attcaaagac gaagtccgtg aattagggac tcagttaggc atgcctgatt
    14821 caatcgtttg gcgtcagcca ttccctggtc ctggtcttgg tattcgtgtt cttggcgaaa
    14881 tcactgaaga caagctagaa atcgtacgcg aatctgatgc tatcttgcgt gaagaaatcg
    14941 cagctgctgg acttgaccgt gatatctggc aatatttcac tgttcttcca gggatccgct
    15001 ctgtcggtgt tatgggtgac ggacgtactt acgactacac agtcggtatc cgtgctgtaa
    15061 cttcaatcga cggaatgacc gctgactttg cccgtatccc atgggaagtc ttgcaaaaaa
    15121 tctctgtacg tatcgtaaac gaagtagccc acgtcaaccg tatcgtgtat gacatcactt
    15181 ctaaaccacc agcaacagtg gagtgggaat aaagagccaa cataatatgg agggggaatc
    15241 cctataaatc aacattcttc aatagctaaa atgcttgatt ttcattaaaa ataagatggt
    15301 tccctaccaa aagagactat aatataagtt aatttgtttc attcttatca taaaaagaag
    15361 ttctcgaaaa acgaactgtt ttccgagaac ttttttctat ccatctaagt gctcaaatca
    15421 tattcacgac ggaattctaa ggttaccctt tcttaaacaa actttatgtt atctgcacaa
    15481 ctttttgcaa ttttttcatt gcttcttacg aaatcgttga actctcttta gcataataca
    15541 aagtgtagat gaagtctggt gctttactcg ttttggtaaa aatgactgga aatcgtttct
    15601 ggttattttt ggcaaaggcg aggtgcaggt tcgattcctg ccttctacgc taacgatgtg
    15661 gctgaaggga acctccgatc ttgaatcgaa acgatccgca gtctattgtt tcaccaatga
    15721 aacaaaatca acgttcgtcc aagcatttcc tgaagccttg ctataaaccc caatttcaca
    15781 aacacctgtt gtaattggaa tgtctttgat tgtaattttt gtccattcac ttgttttaac
    15841 aaccgcagta tttagttccg ctccaccata atttttgata tataactgat tagtatcaaa
    15901 ctctccccca gacattacat aggcactcaa tgtgtaagtt cctttgctta tgccactaac
    15961 attttgataa gctgatgcct cgtatgcttg atcctgccaa aaagttagtt tgtagtcccc
    16021 aaaaaaagtg cccgcttccg ttttgatcgt acccttagcg gttccttgtt gaaaccaggt
    16081 ttgccaattg gaaggactgg tcgttggtcc atcgctttcg aaacttgcat ttttaactag
    16141 atttatttgc gcccctgctt gtttaaacgc acgcaagctt ggcaaggcgt ttccagcaaa
    16201 atcaaacatc gcttggttgt cccattcgtt tcccatagtg gattgaacac tgagataatt
    16261 catacctgct tgtgttgccc aactcacatt tccgttgtac caaagaggtt cccaatagaa
    16321 gaagcccgcc ccgcgattat tcggtactgc ctgaatccga tcggcaagat ccagcaaata
    16381 ctctgcttgt cctgtcggtg ttggttcata tccccccgca gctgcttcgg cttcaccaaa
    16441 agcatttggc atagtatcgg cattcgctgt tgtatgccca taagctgttt caactacgat
    16501 cacttcttta ccataccgtt ggctgatcgc attcatattg gtttgaagtt cattcattga
    16561 accgtgccaa tacggataat acgacactcc aattaaatca aatgacacac ctcgactcgt
    16621 gatttcatca aaccaccaag taaatgctcc tgtatcgcca ccatcagcta agtggagcat
    16681 tactggagtt gttttaggtt gagtgtctcg aacagcttgg ataccagact tcaaaaaagc
    16741 agccaatcga tcaaattctc ccccgccttc tccccaagac ttgccataag gccagagcat
    16801 tccgctattc agttcattgc cgatctgaac catatcagga taaacgtcca gctgcttcat
    16861 ttccgacaga acgttctttg tatagtcgta caatgccgta ttcatttctt caaaggtcag
    16921 accttgccat gctttaggaa gattctgttt cccaggatca acccaaaaat cactgtagtg
    16981 aaaatccagc agcaccttaa gtcccagatt ttttgctcgt tttgcaaggt caatcgtcgt
    17041 ttccagatca tttgtccctg ctccataact ttctccatta ctattataag gatcctgcca
    17101 caaacgcagt cgaacatagt tgacaccatt attttttaaa atctctaagg catcttttga
    17161 aatatcgtct tcataatatt ttgcaccaga tttttccata tctgacagga tcgaaatatc
    17221 tgctccgcga ataaattcgc tggcagatgc ttcaacagtt tttgaaacag cccccaaagc
    17281 ccctaacaca gctgcaaata aaacagcttt acgcaaccaa attcccattt tcattatggc
    17341 tcctccttta ttgaaattgg ttcagaataa actgaacagc tttttcagga tctctcgtta
    17401 atccgatata ttctgctcct tttgtcgcaa ccagcttgat tcctaagcga tcggtgtctt
    17461 ctttgatatc atcatagtaa gaattgactt gcggtgctaa gaccaccata tcaaaatcct
    17521 gcagcatttc gtaatgagaa ccataagcac ccgccattgc actgatcggt gctttcgatt
    17581 cagatgcacc tttttttaaa gcattagcta acatcgcact tgtccctgcg ccagcacaaa
    17641 gtaccaacac attgatttct ttatttgcat cgctgataac ttgactcgct gccacaggta
    17701 cttcctcacg tacatcggca gaagcacctt cctcggttgc gatcacctct gctttttgtg
    17761 cttctttctc tactaagaca gcatcgtatg ctttgcaaaa tggtagatag atcaggaagt
    17821 caacgacgat tagaaccgct gctagaacaa aagctaagat acccatgccc gttcctagaa
    17881 ccaaaccgat cggtgctgga gttgcccaag gcaaaacgta cataaagctg ttcatgccca
    17941 atacatcgac aaaaaacttg aacaggcaaa cgtttacaac aggtgttaac aaaaatggaa
    18001 taaaaaaata agggttcaaa atcatgggtg ccgcaaatag caatggttcg ttgacagcga
    18061 aacttactgg aataaaggaa gctttcccaa ctgccttcaa ctgcttagac ttagcaaaga
    18121 gtaggaagat aaaaggaaca accaatgttg ccccagttcc tcccatagtt cctacaaaat
    18181 tccctaaacc caccgtcagt actttagaag cttgttcacc ggaattgaac aattgcagat
    18241 tggtttccac attggcatag ataattgccg caattgccgg ctccacgatc gacggtccat
    18301 gaacaccgac aaaccaaaac agcgccatcg ctccccagat aatagcgatt cctaagtacc
    18361 cgtcagctgc tgtaaatagc ggctgcagca ccgtaatgat tgcttctgca aatgagattt
    18421 ggaagatttg tcgaatgcct aaatcaatta aaacggccgc aaatactgaa aaagagaaag
    18481 gaaaaatatc tcggaaggtt tgcgaaatcg ttccaggaac ttctttaggc attttaatgg
    18541 taatatcttt cagcacacaa aacttatata aattaactgt taaaaatgca gcgacaaagg
    18601 aagccagtag tccctttgtc cccatatatt ccgttgaaaa tccaccttca atcggagaga
    18661 cgcttaacaa caggaaactt accacagagg ccagcatcac agaggtggtg ttgattactt
    18721 tgtttttcgg taagcgacga ttcatcgaat cagccaagca gcgagcagtg gttgctgaaa
    18781 cgagtaaccc tacaacaccc atcgaataat tatagacttt ccatagccat gtggagacag
    18841 attcaggcca ttggtaacca aaaatctcag gaaccgcagc aatcaaaatg aaaatgcttg
    18901 aaaataaaat agctggcatc gctgttaaaa atccgtcacg aacggctcct aaataactat
    18961 tacgtgagat tttttcaaaa aatggtttcc ctttttcgat ttgtttaatg attccatcca
    19021 tagcgttctt ctcctatcct tgattctgtc tgtacagtac aataaagtct tgaatcagat
    19081 ctctcagcaa taaagtggtc attaaatgat cctgcccatg gataaaaatg aatcccatct
    19141 ccatctcttt cccagatgct tcttccgcta aaatttttgt ttgcgaatta tgtgcaaggg
    19201 ttaaattttc gtcggcttct ttcaacgccg aatcaacatg ttcaaagttt ccttgtctta
    19261 cttctttcaa taatttcaat aatgtactgc gagcgtcgcc tgaatatgca acgatctcaa
    19321 aaccaagcat ttgtaattcc tctttattca tcttttcttc ctccattatc acttattaaa
    19381 taatcgtttt ggtttcactg accaacttgt accagtaagc tgattctttt gggtaacgct
    19441 tctgggtttc aaaatcaaca taaaacaagc catacctttt gttataccca tttgtccatg
    19501 agaaaagatc cataagcgac cacaaaaaat accccttcac atttaccccg gctgttatcg
    19561 ccttgcttaa ggattccaaa tagactctca aatagtcgat tctcggctga tccataataa
    19621 tgccatcttc aaactgatct ttatacccca tcccgttctc agtgatatag attttgttgt
    19681 aatgtgggta atcgctttta attcgcagca acaagtcata tagaccttct ggataaatca
    19741 gccagtccca atcagttcga ggaattcctt ctttataaat acgttctccg attcccttca
    19801 ctttataaac agaggtccct ttttcacccg taccgttatg atggattgca ttttctccat
    19861 cgtaagcttt gacaaaatgg cattggtagt gattgatccc taagtagtca ttacgcgtgg
    19921 atgctttttt caattcaacg aaatcttctt caggaaaatg atagctggcc tgatttgctt
    19981 cacatatctc atcaagagca gtcaaggttt cagttgaata atagcctaaa taggtagcat
    20041 ctaataagaa acggatcgac aatgcatcgt ctaaaaaggc agcatgctta tcttccggcg
    20101 cgtctgttgc cgcatatttc gtctctaatg agtgaactac accaatttca cccggaagtt
    20161 cgttttcttt gaaatagttc actacacggg catgagcaac catcatatta tgaagacagg
    20221 cgacgatctt tgtaaaatca tatttgattc ctggtgggaa aacacctaac aaatattgat
    20281 tggttgcaac tggatagatt tcattaaagg tactccacac cttgacttct ttgaattcat
    20341 gaaaacaaaa aatggcatag gaaacaaatg cttctattgt ttctcgattc aaaaaatctc
    20401 catggtcaaa caaacgttta ggggtatcaa aatgatgcaa cgtcacaaac ggcgttacgt
    20461 ttcttttctt acattcttcg aacacacgat gataaaaagc gattccttct ggatttggtt
    20521 tcccagctcc atcaggaaaa atgcggctcc aagcaatgga taatcttaga ccatttacac
    20581 caaaacgttc acacaattca atgtcctttg catattgatg ataaaaatca cttgccggat
    20641 cgggactgaa tcgcccttgt tcctctaaaa agtcatccca agcaacagcg cctttgccac
    20701 cttccttagt ggctccttca acctgatagg ctgctgtggc tccgccaaaa ataaaatctt
    20761 ctggtaattt aagcatgtac atctctcctc aaatctataa tcagttttcg ctctttctaa
    20821 ctgattgcat tgtaaacgca tccgaaacat ttgtcaacaa aaacaaacac aaataatcat
    20881 aaaaaccaga aaacagcaat aaaatctatt gtaacaaaca taaaaaccac cctaaatgtt
    20941 tacttttgtt cgaattagat tgactgttgt ccgtttatat gatagattat ccttaaatta
    21001 gaggtgaaga caatgttgaa aaaagaaaga ttgcttacta tcgtagaaat ggtcaataaa
    21061 aaaggaatcc ttaccgttaa tgaaatcatc aatcaattag atgtttctga tatgaccgtt
    21121 cgcagagatt tagatgaatt agaaaaatcg ggcaagctgc tgcgtgtcca tggaggggct
    21181 caaagtcttt cctacacttt agatcaagaa ctttctcaca ctgaaaaagc cactctgcag
    21241 attcaagaaa aaaaagaaat tgctgccaca gcagcctcct tgattcacga gggagaaacg
    21301 attttcttag gaccaggtac aacaatcgaa ctattggcga cgcatttact gaacaagaaa
    21361 attcggatca ttacaaataa ttatccagtt tttgatattc ttgctcaagc agatggacca
    21421 gaaattctac tgattggcgg tgatttccgg aaaaataccg gcgcatttgt cggaccgatc
    21481 accaacgaca atttaagaag actaaagttt acaaaagcat ttatcagttc aaatggcgtt
    21541 cacaacgaag cgatctctac ttacagtacc gaagaaggag aagcacagca gatcgctcta
    21601 aataattccc gtaagaaata tctactcgtt gataataaaa aattcaatcg agatgatttt
    21661 tatgtgtttt ataatttgca tgatttcgat ctgctcatta cggatcgcca gatctcgcgg
    21721 gaggttcaag agcactatgc acaatatgtc gatctgcaaa ttgctgattc aaccgaaaat
    21781 accactatag gagcgtaact atgaaaggga ttatatttga tttcaacgga acaatgtttt
    21841 tggactctca tctgcatgaa gctgcttggc tgcatatgat acatgatcat acaaaagatg
    21901 gtttatcaga cgaggatatt ttaaaaaata ttcatggtcg gacgaatacg gagattttga
    21961 aacatttcat ttcagagcat ttgaccgcct ctgagatcgc cgtattgtca gaagaaaaag
    22021 aaagctatta tcgtatgctt tgcttgcaaa atgaaaacga gcttcagttg acaaaagggc
    22081 ttgaagcgac tcttgaccag ctgactgacc aacagattcc attaacgatt gccacggcta
    22141 ctgtcaaaga gaatgttgct ttttattttg atattttcga tttagcccgc tggtttgact
    22201 gggacaagat tgtctatgac gacggctctt tccctgggaa accgcaaccg gatatttttc
    22261 ttaaagcggc agacaaactg agcttagctc cggctgattg tcttgttatc gaagatgctt
    22321 actcaggatt acttgctgcc aaacgtgcgg ggatcggaac aattattgca attgatccat
    22381 tcggtaaaaa caaaacagta tttgaacagg cacagctaaa taaagatgga accatcaaag
    22441 attttactgg tttttggaca gagcacctgt ctgccacatc cgttaattct taaacgattc
    22501 aactaactac caaagaaaag caactgaaat gcacacctcc ttttataaaa aactgccagt
    22561 gaaacgacat gaaagataca ctgctatatc cttagtgaaa tatagcagtg tatctttgac
    22621 tttttcattc ttttaaccat atgacgcgtt catctctatc tataattaac attaaaaata
    22681 gtgttttcac attatttctc aaaccaatct taaaaatcat caggagtact tttttgatat
    22741 acttttatta atgctgggcg taatggttaa aagatttcca ctagttcctc tccaccaagc
    22801 tgagttgaaa aatgcttatc tgtaaacaac atagacaaag aggaaataaa cgtttccgta
    22861 tctttctctg tcccaaggat attaattaat tttcctacta ggtaagtatc attaaactta
    22921 agatctgcct tcacatcaaa agttttgata aaagctttta tcatgcgtac aattttgtct
    22981 ttatcgccat aattgatttc accactcata tcaatatagt cactaatccc tgaagaaata
    23041 atggcaactg cttcttcttt cgccttatca gataattcat ttaatgactg ggattgattg
    23101 atgacttctt gagttttatc tgttacgtaa tctctcatta tcctatcggc atactccgca
    23161 aagtaatctt gaaaaaattc ttctacaggg taaccaactt cgttttccca accaggagaa
    23221 atttcattat cagtacttat taaatctatt aaaaaccaac ccgttctctg agtgttgatt
    23281 ctataatatt tttaatttcc tatataaatc aaaaatatta tagaatcaat aagctaatta
    23341 agaaacaaac tttagaaact ctagaagaac ttctgaattt atatagaagt gtgtagctac
    23401 ttactaaata tacaaaagtt aaaataaaat actctggata ctctttatac tgcattaaag
    23461 ttagtaatat taacagcaaa gatatcgaaa aaacaataac ataatatgtg tcaaaatttt
    23521 tcataataag aactccctta ctgttgcata ctaacataat ccaaaaactc ttctacttca
    23581 gctaaagaca ttccactttc tttagtccaa aaaacagttt tttctatcga tccttctctg
    23641 taaactgtaa atgtcggagt cgagttaatt gagtagcgac tcttgaattc tttccactca
    23701 ttttcatctt ctctaacttt tgctacattc acgtaataaa tattttcttt tcccttttct
    23761 accagcatat cgaatagctt cctgcttttg atgatcgatt tcttgtgcta catctagtaa
    23821 attttcatca atttctatat cataaagtat ttcactagtg tctacatcca caatatatac
    23881 tttttatcag tagcaatttt tgagtaatcc gttaaaatga tctcagtctc gtcactttca
    23941 tcagtataaa tatacgttaa caatgaactc accttttcaa tcggtgttag ctctaagcct
    24001 actgtatttt ttactattga gtcattattt accaccataa cttcgtactg tatataccat
    24061 cctatttggt ttccatcaat atcagtaatt ggctcactag ctaatggagt ctccggaaca
    24121 atattttcct gatatttctt aaactcttct gtagcacgaa aaaggttatc atgttcctca
    24181 cttgaaactg gttgagtcat agaaaatgtt acttggctaa acagagggaa aataaacact
    24241 acaatcaaca aagtataaaa aaaagccctt ctttttcatt ttttatcttc caatcacaaa
    24301 ttgaaaaact tattcctgca actttgagtc tagtatcaca atcgactgac tacaactatt
    24361 catttacatt ctactttaag ttcctcctta accattaatt ttattaaaga tacttgtaat
    24421 agtgattctc acaaaatttc atgaaaaata ctttattact gtatctaaaa ttccaataca
    24481 caacttatcg actattatct gaaaaccaaa acttattatt ccttgatgtt attctagcaa
    24541 tagtaactgc ttttttattt ttttgcatat tttttcggcg ttacttttta aagttaactt
    24601 tttcgacaaa tagaaagaga tacgaatcac tgattttttt gaagaaaaga aatggagtga
    24661 gctgaacgtt agagatattg ggagctttcg taactgctta ttaaacgata atggttattt
    24721 gcaaccgtat tgctcgcttc tacatgaaat atttcatgaa atatttcgca aaacctttga
    24781 ttatgtagtt acgtttccat tcttgactaa aaatgtttca aaaacgaaca aaggtgttaa
    24841 aaggtaaagt gatcaatact actggtcaaa ataagaggtt gaaagggaat tgaaaaataa
    24901 ctacagataa tacttttata aacgtacgtg ctttgtggtt ctgtagttaa ctatataact
    24961 ggaattaggg aatggtatca atttgaaaag aaagattcta ccaatttatc atatgttaaa
    25021 aatggaaaat caacagcagt tctttcggaa accttacaca aaaaccgatt attgaaaata
    25081 gccgaaaaac gtttttcggc tattttttta attaagtttc cttcttctta ccttttccca
    25141 ctattccgtt tcacatcttt gatgatcttc agcgcacgtt ttcgtgtttt gatatttggg
    25201 cttttcaagc ggcggtatgc acttgagata tctgtttttt ccatatggac gacccacttt
    25261 cttctttgat tgtagtcatg ctaaaaaaca caaatcgaaa caattacgat ttgtgtttta
    25321 aatggtatca aaagttaaaa tgaaagtcaa cttgttaaaa cagtcgatca gactagctga
    25381 ttgactccta tctctggaaa taaattttca ttattacgtt ttagctacct aaaaaaggcc
    25441 ctcttgtccg ttaactttaa tatctattgt tatagctagt ttacctattg aacgtactgt
    25501 gtgttcgata gaacac
//
LOCUS       DACEUZ010000028        25358 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.028, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000028 DACEUZ010000000
VERSION     DACEUZ010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25358
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.028"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(132..509)
                     /locus_tag="IXG44_000983"
     CDS             complement(132..509)
                     /locus_tag="IXG44_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1657716.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            765..1493
                     /locus_tag="IXG44_000984"
     CDS             765..1493
                     /locus_tag="IXG44_000984"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HAQ1657717.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            1493..1747
                     /gene="purS"
                     /locus_tag="IXG44_000985"
     CDS             1493..1747
                     /gene="purS"
                     /locus_tag="IXG44_000985"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HAQ1657718.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            1749..2420
                     /gene="purQ"
                     /locus_tag="IXG44_000986"
     CDS             1749..2420
                     /gene="purQ"
                     /locus_tag="IXG44_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HAQ1657719.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            2421..4643
                     /gene="purL"
                     /locus_tag="IXG44_000987"
     CDS             2421..4643
                     /gene="purL"
                     /locus_tag="IXG44_000987"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HAQ1657720.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            4628..6067
                     /locus_tag="IXG44_000988"
     CDS             4628..6067
                     /locus_tag="IXG44_000988"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010768528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HAQ1657721.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            6099..7142
                     /locus_tag="IXG44_000989"
     CDS             6099..7142
                     /locus_tag="IXG44_000989"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HAQ1657722.1"
                     /translation="MANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGFGGC
                     FDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEPLYF
                     LDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFAVGI
                     AEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKKTLG
                     EELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSWPIL
                     PIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVQESKVKEIVHEIEQAGEKVYEIGKVIA
                     KEKHRVVFIGEER"
     gene            7139..7717
                     /gene="purN"
                     /locus_tag="IXG44_000990"
     CDS             7139..7717
                     /gene="purN"
                     /locus_tag="IXG44_000990"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HAQ1657723.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            7714..9249
                     /gene="purH"
                     /locus_tag="IXG44_000991"
     CDS             7714..9249
                     /gene="purH"
                     /locus_tag="IXG44_000991"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HAQ1657724.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            9265..10539
                     /gene="purD"
                     /locus_tag="IXG44_000992"
     CDS             9265..10539
                     /gene="purD"
                     /locus_tag="IXG44_000992"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HAQ1657725.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            10661..11575
                     /locus_tag="IXG44_000993"
     CDS             10661..11575
                     /locus_tag="IXG44_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HAQ1657726.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(11708..13273)
                     /locus_tag="IXG44_000994"
     CDS             complement(11708..13273)
                     /locus_tag="IXG44_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAQ1657727.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(13266..14717)
                     /locus_tag="IXG44_000995"
     CDS             complement(13266..14717)
                     /locus_tag="IXG44_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ1657728.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(14731..15747)
                     /locus_tag="IXG44_000996"
     CDS             complement(14731..15747)
                     /locus_tag="IXG44_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1657729.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            15967..17055
                     /locus_tag="IXG44_000997"
     CDS             15967..17055
                     /locus_tag="IXG44_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAQ1657730.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            17109..18635
                     /locus_tag="IXG44_000998"
     CDS             17109..18635
                     /locus_tag="IXG44_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAQ1657731.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            18637..19842
                     /locus_tag="IXG44_000999"
     CDS             18637..19842
                     /locus_tag="IXG44_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1657732.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            20099..21076
                     /gene="guaC"
                     /locus_tag="IXG44_001000"
     CDS             20099..21076
                     /gene="guaC"
                     /locus_tag="IXG44_001000"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HAQ1657733.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            21340..22764
                     /locus_tag="IXG44_001001"
     CDS             21340..22764
                     /locus_tag="IXG44_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAQ1657734.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            23225..24334
                     /gene="pdhA"
                     /locus_tag="IXG44_001002"
     CDS             23225..24334
                     /gene="pdhA"
                     /locus_tag="IXG44_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HAQ1657735.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            24337..25314
                     /locus_tag="IXG44_001003"
     CDS             24337..25314
                     /locus_tag="IXG44_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HAQ1657736.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
BASE COUNT         8202 a         4317 c         5695 g         7144 t
ORIGIN      
        1 caaaaaagaa gcagatccgt tagcggttct gcttctttta gtttattata ttttcttatc
       61 aataatagga acctgatttt aaaagcggct acctctttac cgtgcctctt tacatacaaa
      121 aaatagagga attaagcgta aatggcttgg ctattataac taaaatgctg gttatggaga
      181 tgttgcagca agttttcgag aaaagcgtat tccatctctt gtccattgaa taagatctgg
      241 atacgataat gatttttgaa acgcttctgt tcatgaatct gactgattga aagttcgcct
      301 ttgtgttctg tctgataaag aaaagtcctt ttacggcatt cagcacagcg cttggttgct
      361 cggcggattt ttcggaatgt ataaaaacta gtatctttat tgcataaata aacgtctttg
      421 atcgacagca aatcattata tagacaggaa cgttcttcag ctgttaattc tgataaaaaa
      481 agctgacctt gttttattgc atcttccatc ggcaagtgcc tcctcgaacc cattatccaa
      541 atttttagtt aattctaata taccatcatt aagtataaat gtaaacgctt gttgatggta
      601 taagattttc gtttctgata aaacacgaac attaaaagaa tctaatgcct taaatgtaca
      661 tatttttgtt gatttataaa aagatgattg ctaaaattta aagtgaaaaa acgaacaaaa
      721 aacgatgtat ttgcttttaa tgtaaataaa aaggagagac gtacatggag aagaaagaac
      781 ttttgtatga aggaaaagcc aaaaaaatat atcttacttc tgatgagaac ttgatttgga
      841 tagaatattt agatcaagca actgcgttga atggcgcgag gaaagaccag ataagtggaa
      901 aaggagaatt gaataatcaa atcacaagtg caattttccg ttatctgact caaagaggaa
      961 taaaaaatca ttttattgaa caattgtcta aaaccgaaca attagcaaaa aaagtacaaa
     1021 ttatccctct ggaagtggtt atccgaaata ttgcagcagg aagtttctca aaacgtctag
     1081 gtgttcccga aggtaaagaa ttcatcaaac caatcattga attctactat aaggatgacc
     1141 agttggatga tccatttatc aatgaagagc atatctattt cttacaactt gcaaagccgg
     1201 aagaaatcag tgagctgaaa gcccaagcgt tggctattaa tgaaattttg accgaattat
     1261 ttgctaaggt cggtttggtt ttagtagatt tcaagctaga atttggccgg tcgaatggac
     1321 aaattttgct tgcggatgaa gtaacaccgg atacttgccg attatgggat gaacgtacga
     1381 atgaacatat ggacaaagac gtttaccgca gagatctagg tgatttgatc ccagtctatc
     1441 aagaagtata taaccgtcta tcggaactaa ctgaagaaaa ggagcgagta tgatgtattt
     1501 cgtaaaagta tatgtaacct ataaagattc agtgttagat cctcaagggg aggcagtaaa
     1561 aaaagcagtc catcgtatgg gatatgaaac aatcgaagat atccgaatag gaaaatactt
     1621 tgagatacaa cttgctgatg cagaagaacc aatcgataca gtgattgaaa cgatttgtga
     1681 caagctgctt gcaaatgtaa atatggaaac ataccgatat gaaatcaaaa aaatggagga
     1741 agtataacat gaaatttgcg gtcatcgttt ttccaggttc aaattgtgac gcagatatgt
     1801 tatgggcagt tcgcgagatc atgggcgctg atgccgaatt tgtacggcat gatgagtcct
     1861 ctttagaagg atttgatggg gttttactgc cgggaggatt ctcctatggt gactatttga
     1921 gatgcggtgc gattgctcgg ttttctacaa tcatggaaga agtgattcgt tttgctgaag
     1981 aagggaaacc agtattcggt acatgcaatg gattccagat attgacagaa gcaggacttt
     2041 tgccaggtgt actgagacga aatgagtctt tgcattttat ttgcaaaact gtaccattga
     2101 aagtcaaccc caagacggcc tttacttcag agtatgaagc cgacgaaata atctatcttc
     2161 cagttgctca tggggaagga aattatttct gtgacgaaga aacacttcaa gaactaaaaa
     2221 aaaataatca gattgccttt acttataatg acaacatcaa cggcagcgtg gaaaacattg
     2281 cagggattac gaacaaagca gggaatgtgt taggaatgat gcctcatcca gaacgtgcag
     2341 tagaagactt gctaggatca gaagatggga aacggttctt tgcttccatt ttaaaaaact
     2401 tcggaaaggt gaccgtatag atgaaaacat tgaaagaacc gacagctgaa gagatcaaga
     2461 aaaaaagaat ctatgccgaa tggggattga ctgatgaaga atatcgaatg atcgaagaag
     2521 atatcttagg aagaatgcct aattatacgg aaactggact gttttctgtt atgtggagcg
     2581 aacattgttc ctataaaaat tctaaaccgg ttctacgcaa atttcctact agtggacctc
     2641 atgtgttgca aggccctgga gaaggcgcag gcatcgtcga catcggtgat ggactagcag
     2701 ttgtcttcaa agcagaaagt cacaatcatc cttcagccgt ggagccttat gaaggagctg
     2761 cgacaggagt tggcggaatc atcagagata ttttcagcat gggggctcgt ccaattgcgt
     2821 tgctagattc attgaggttc ggcgaattag ataatccaag aacgaaatat ttactagaag
     2881 aagtagttgc aggtatcggc ggatacggca attgtatcgg cataccgact gtaggcggcg
     2941 aaattgcatt tgatccttgt tatgaaggaa acccattagt caatgcaatg tgcgtaggtt
     3001 tgattgaaca caaacatatc cagaaagggc aagccgctgg cgtcggtaat tcgatcatgt
     3061 atgtaggtgc aaaaacaggt cgcgacggta tccacggagc aacttttgcc tcagaagaat
     3121 ttaatcaaga agaagaacag caacgttctg ctgtacaagt aggcgatcct tttatggaaa
     3181 agctgctgct tgaagcgtgt ttggaattaa tcttgcatca ttcggattct ctaataggta
     3241 tacaggatat gggagcagca ggacttgttt catctagtgc ggaaatggct tcaaaagctg
     3301 gctcaggact tatattgaca ttagatgaag tcccacaaag agaaactggg atgacacctt
     3361 atgaaatgat gttatcggaa tcacaagaac ggatgttgat ttgtgttaaa tcaggagagg
     3421 aagaaaaaat ccaagaacta tttcagaagt atgatctaga tgcagtcact atcggtaaag
     3481 taacagacga cgggcaatat cggctatatc atcaaggaga agaagtagca aatctgccag
     3541 tcgatgcatt agctgaagat gcaccagttt atcataaaga aatgaaagaa ccagctcgaa
     3601 tcgtagaatt tcagcagtta gctccttatc agccagttat cgaagaacct ggacaagtat
     3661 tgcttgatct gctgcagcaa ccgacgattg cttcaaaacg ttcaatctac gaaacttatg
     3721 attcacaagt acaaaccaat acagttgtcc gtccaggaag cgatgctgct gtgatgaggg
     3781 ttagagggac gaataaagcg ttagctatga ccacagactg caacgcgcgc tatctgtatt
     3841 tggatccaaa aatcggagga caaatagcag tggcagaagc tgctagaaat attattgcta
     3901 gcggtggtaa gcccttggcg atcaccgact gcctgaacta tggttctcct gataaaccag
     3961 aagttttttg ggaattatcg acttcagctg acggaattgc tgctgcgtgt gaaacgttag
     4021 gaactcctgt tatttcagga aatgtctctt tatataatga aacggatggt caagcaatct
     4081 atccaacacc aatgattggt atggtcggat tgattgagga tcataaacat atcactactc
     4141 aggaattcaa aaaatcaggg gatctaatct atatattagg taagacgttt gcagattttg
     4201 atggcagtga attacaaaag atgcagcttg gaagaatcga aggtgtgatc cgaaactttg
     4261 atctatccat tgaaaagaga aaccaagagt tagtattgac agccatccaa aacggattga
     4321 ttgaaagtgc tcatgactgt tcagaaggtg gcttggcaat tgcactggca gaatcagctt
     4381 tcaaacatca gctagggata tctgtacagt ttgaattgtc ctctgctcaa ttatttgcag
     4441 aaactcaatc aagatttgtg ttgacagtcg caccggaaaa taagacacga ttcgaagaaa
     4501 tgatgggaga tgctgctgtt ttagcaggta aggtgacgga tgaagcgata atcgaaattt
     4561 cagcaactga cggtcagatc aaaatagaaa ctgcagtagc aagaaagtgc tgggaggatg
     4621 caatcgtatg tcttatgaag tgaagagttt aaatgaagaa tgcgggatct tcggtgtttg
     4681 gggtcatcca gacgccgcta gtgtggcata cttcgggctc catagtcttc agcatcgagg
     4741 acaagaaggt gcaggtatcg tgtccaatga tcgaggaaag cttaaggggc atcgagattt
     4801 ggggctgctt tctgaggtat tcaaagatca aagaaaattg cagcagttaa caggagaagc
     4861 tgcaatcgga catgtacgtt atgcaacagc aggcaatgga agtgtggaca atattcagcc
     4921 ttttctattc aaatttcatg atcagtcgat cggcttagcg cataatggta acttgatcaa
     4981 tgctcgttcc cttagaaaag agttagagga aaatggggcg atcttccatt ctaactccga
     5041 tacggagata ttgatgcatt tgatccgacg aagtgaagaa acagattttt taaataaatt
     5101 aaaaagtgct ttgaatgaag tcaaaggtgg ctttgcctat ttattaatga cagaaacggc
     5161 aatgattgcc gctttggacc caaatggttt tcgtccatta gcaatcggac aaatgaaaaa
     5221 cggagcatac gtgattgcct cggaaacttg tgcattagaa gttattgggg cacgttttat
     5281 aagagatgtc cagcctggtg agatagtgat catcaatgac gaaggaatcc agattgattg
     5341 ctttacgaag gacacgcagc tttctatttg ttcgatggaa tatatctatt ttgccagacc
     5401 agattcaaac atcgcaggga tcaatgtgca tactgcacgg aaaaatatgg gacggaattt
     5461 agcgaaagaa tcgccagtaa aagcagatat ggtcgttggc gtacctaatt cttctttgtc
     5521 agccgcaagc ggttatgcgg aagctagtgg aatcccttat gaaatcggat tagtaaaaaa
     5581 tcaatacatt gcccgaacat ttatccagcc aacgcctgag ctaagagagc aaggtgttcg
     5641 aatgaaatta tctgctgtac gaggagtagt ggaaggtaaa aaggtgatta tggtcgatga
     5701 ttcaattgtt cggggtacga caagcagacg gatcgtccat cttttgaaag aagcaggggc
     5761 aaaagaagtc catgtgagga ttgcttctcc tcctttgaaa tatccatgtt tttacgggat
     5821 tgatattcaa acgcgtaaag aactcattgc ggcaaatcac acgattgatg aaatcaaaga
     5881 atgtatcggt gcagattctt taggtttttt aagtgaagag ggtctgatcg aatcgatcgg
     5941 tctggaaaaa gatgctccgt attcaggatt gtgtatggct tatttcaacg gagattatcc
     6001 gacgccgtta tatgactatg aagaaaaata tcaggaatca ctaaaagaaa aagtatcgtt
     6061 cttttaaaag cagaccaaga agggagtata tgaattcaat ggcaaatgca tacgcaaaag
     6121 ctggcgtaga tgtcgaagct ggatatgaag tagtagaacg gatcaagaag catgtcaaaa
     6181 aaacggaacg tttaggtatg atgggcgcta taggaggatt cggcggttgt tttgatttat
     6241 cggtctttca attaaaagag ccagtccttg tctcggggac cgatggcgtg gggacgaaat
     6301 tagcagtggc aatcgaagca gacaaacacg atacaatcgg gatcgactgc gtagcgatgt
     6361 gtgtcaatga tatcttagca caaggcgcag agccgttata tttcttagac tacttagcaa
     6421 cggggaaaaa tgatccgtta cgtttggaaa atgtagtaaa aggtgtagca gatggatgtg
     6481 ttcaaggaaa tatggcgctg attggcggag aaacagctga aatgcctggt atgtactcag
     6541 gtaatgatta tgatctagcc ggatttgctg tagggatagc tgaaaaatct caattattga
     6601 caggagaaac gatcaaccca ggagatgtgt tgcttggaat agcatctagc gggattcatt
     6661 caaatgggta ctctcttgtt cgcaaaattt tctttgagca gcagcagttt tctttatcat
     6721 caaaactgcc agaattgaaa aaaacacttg gcgaagaatt gcttgagcca acgaaaatct
     6781 atgtttcttc cttgcttccg ctgatcaaag aaaagaaaat aaaaggagct gcacatatca
     6841 ctggtggagg gtttgtagaa aatatcccgc gaatgttacc agaatctatg gctgcacaaa
     6901 tcaaacttgg ctcatggccg attcttccaa tattcggctg tttacaaaaa tatggtgaaa
     6961 ttcctgagat ggagatgtat gaaatcttca atatgggcat cggtatggtc ctggcagtcc
     7021 aagaatcgaa agtaaaagaa atagtacacg aaattgagca agctggcgaa aaagtttatg
     7081 aaatcgggaa agtgatcgcc aaagaaaaac atcgtgtggt gtttatagga gaagaacgat
     7141 gagaatcgct gtttttgctt ctggaaacgg aagcaacttt caagcgctag ccgattatct
     7201 atcgaaaaaa ggattggaat cttcgattga ctggttgttt tgtgatcagc ctgaagcata
     7261 tgttttgaaa cgtgcaactg cgttgagtgt accggcagat tgtttttcac ctaaagaatt
     7321 tgattcaaaa aaagagtatg aagaagcaat tttgcataaa ttgaaagaaa aaaagatcga
     7381 tttgatcgta ttagctggat atatgcgaat cattggaccg gtattgctga aaaattatga
     7441 taaaagaatc atcaatatcc atccgtctct tttacctgcc ttccctggtt tgcatggcat
     7501 acgtgatgcc tttgaagctg gagtaaaaga aacgggtgtc acaattcatt atatcgacca
     7561 aggtgtagat actggaccaa tcatccggca ggaaaaagtg agaattgaac aggaggacac
     7621 gttcgattct ttagaagaaa aaatccatag agtagagcat cgaatctatc cagaagtgat
     7681 tagtgaaatt attgaaaatg gaggctatct gaaatgacaa gagcattgat cagtgtatca
     7741 gataaaactg ggattgtagc attcgcccaa ggtttgattg aacaaggagt agaaatcatt
     7801 tcaacaggag gaacaaagca tgcgttggat aaagctggga tcaaaacgct ttcgatcgaa
     7861 gaagtgacag ggtttcctga aatgatggat ggccgtgtca aaacacttca tcctaaaatc
     7921 catggtggtc ttttaggaag acgcgatctc cctagccata tggaggcaat ggaaacggaa
     7981 aatatccaac caatcgactt tgtttgtgtc aatttatatc ctttcaaaga aacgattatg
     8041 aaaccagatg tcgaaatagc agaagctatt gaaaacattg atatcggtgg tcctagtatg
     8101 ctccgtagtg cagcaaaaaa ccatgaattt gtcacctctg ttgttgatcc atctgactat
     8161 gaagaagtac tttcagaatt acgtcagtat gggcatactt cactttcttt cagacaaaaa
     8221 ctagcggcaa aagtctttcg tcatactgcg gcatatgatg cactgatcgc tcaatactta
     8281 acggattggg tggaagaaaa agaaccggaa aaattgacac ttacgtatga acgaaaacaa
     8341 acgcttcgtt acggtgaaaa tagccatcaa gccgctgcct tttatcagtc cgctcttctg
     8401 gaaaaatatt cgatcgcagc agcaaaacaa ctccatggga aagaactttc ttataataat
     8461 atccgagatg cagatgctgc gctacggatc atgaaggaat atgacgagcc aacggtagtc
     8521 gccttgaaac acatgaatcc ttgtggtatt gggacagcat ctacgatttt agaagcgtgg
     8581 gaagcagcgt atgaagcaga tcctgtttca atctttggtg ggatcatcgt tttgaatcga
     8641 gaagtagatc tgccaacagc agaacagatg cataagctgt ttctagaaat catcattgcc
     8701 ccttcttata cacaagaagc tttggaatta ctgcaaaaaa agaaaaacat ccgattactg
     8761 acagtagact ttgcgcagga agaaaatcaa aatcgagaag aaacggtctc agtcttaggt
     8821 ggacttcttg tacaagatca agatctggca atagaaaatg aagaagagtg gaaagtcgtg
     8881 acaaaacgta agccgactcc agcagaagca aaagcattag cgtttgcttg gagagctgtc
     8941 aaacatgtga aatctaatgc gatcgtactg gcaaacgaac gtcagacagt aggaatcgga
     9001 gccgggcaga tgaatcgagt cggttcagta aaaattgcga ttgaacaagc gcaagagaaa
     9061 atggaaggtg cagttttagc tagcgatgct tattttccaa tggatgattc ggttgaatat
     9121 gcagccaaac atggtataaa agcaatcatt cagccaggag gaagcatcaa agaccaagat
     9181 tctattgata tggcgaataa atacgatcta gcaatggtct ttactgatgt tcggcatttt
     9241 agacattaga gaggagtcgg ataaatgaac attttggtga tcggtgcagg tggtcgtgaa
     9301 catgcaataa gtaaaaaact tttagccagt ccgaaggtgg atacggtgta ttgtgcgcca
     9361 ggaaatccgg ggatgacaaa agatggtatc aattgtgtcg ctatcagtga gcgagatcat
     9421 tcagcgttaa tccattttgt taagaaagag gctattgatt ggagtatcgt tggtcctgaa
     9481 gttcctttgt taaatgggat cgtcgatgat tttatggcgg aaggattgaa aatcttcggt
     9541 ccgacaaaag cagcagctct gatcgaaggt tcgaaaagtt ttgctaaaca attaatggat
     9601 acttatcaaa tcccgacagc taaatccaga agttttgaat gttacgaaaa agcagcagcg
     9661 tatatcaaag aaaatggtgc gccaatcgta gtgaaagcgg acggcttagc agctgggaaa
     9721 ggtgtagtcg tggctaccac agtgaaggaa gcgctggatg cggcagaaca aatgttgaac
     9781 cagcatcgat ttggggatag tagcaaaaaa atcgtcgtag aagagttttt ggcaggagaa
     9841 gaattctccc ttttggcgtt tgtccgtaat accaaggtgt atccgatggt catttcgcaa
     9901 gatcataagc gggcatttga taacgataaa gggccaaata caggcggaat gggtgcttat
     9961 tcaccagtac cgcaaatttc aaaaaagatg attgaaaaag ctgtggaaga agttttaaaa
    10021 ccagcggctt tgggaatgaa agaactagga cgctctttca cgggtgtttt atatgcgggt
    10081 ttgattgcta cgaaggaagg accaaaagtg atcgaattca atgcgcgttt cggtgatcct
    10141 gaaacgcaag tcgttctttc tcggttgaag agtgactttg cgcaagtaat cgatgattta
    10201 ttagagaatc gtacagtaga gttaaagtgg cagcaagaag gatacaatgt aggcgttgtg
    10261 gttgcagctg ccggttaccc cgaagaatat gagacaggaa tggttctacc agattttctg
    10321 gaagaagagc tttctgttta ctatgctgga gtttcctcaa ataacggcaa actagttgga
    10381 gcaggaggac gtctttattt agtagaagca tacggtgaga cgatcaaaga agcacagaaa
    10441 aaaatttaca ccgcgttaat ggataaaacg acagatggaa cattttatcg tacagatatt
    10501 ggagaaaaag ctgccaatag cgaactgatc gtgaagtaaa tcagctgctt ccatttataa
    10561 aaaatacaac aaacataatc ggaagcgccg attttagtga agccttttca taaagagtgt
    10621 ggtacacttt tgttgtgtat ataaataagg aggagttcag atgaaaaaaa ttcaattagg
    10681 tacttctgat cagaaagcag ctgcagtgat cttaggctgt atgcgtatca acaatgcgga
    10741 agatccagta aaagtgattg agacagctta tgaaaacggc attgattttt ttgaccatgc
    10801 agatatttat ggaaacggtg aatgtgaaga aatctttgca gatgctctag caaaaacatc
    10861 aatcaaacga gaagatcttt ttattcaaac aaaatgtgga atcgttccag gtgtgatgtt
    10921 tgatttttct aaaaaacata tcattgaatc tgtagaaaaa agtttgaccc gtctgaaaat
    10981 ggattatgtg gatgctttac ttcttcatcg tccagataca ttggtagaac cagaggaagt
    11041 agctgaagcg tttgatgaat tggaacggtc aggaaaagtt cgttattttg gcgtcagcaa
    11101 tcaaaaacca atgcaaatcg aattgctgaa aaaatatgtt cgtcaaccat tagtagctaa
    11161 tcagctgcaa tttggcttga aacatactgg aatgatcgat caagggattc atgtcaatat
    11221 gacagatgaa gcaagtatcg atcatgacgg tagtgtactt gattattccc gattgcatga
    11281 tatgacgatc caagcatggt caccatacca gtatggattt tttgaaggtg tatttatcgg
    11341 gaacgaaaaa ttccctgaat taaaccaaac attagaagaa ctagcagcta aatatcaaac
    11401 gacaccaact ggcttggctt ctgcttggat tttacgccat ccagccaata tgcaagtgat
    11461 cgccggtacg atgaacgtag ggcgaatcaa ggaaatcgct caagcttcag aaattaatat
    11521 gtctcgggaa gattggtacg aattgtaccg tgctgctgga aacattttac cttaaagatt
    11581 gtcataagca ggtcaaaagg gctataaatt aaaaagacag aataacttga aagctgtttt
    11641 cagcttcgag atattctgtc tttttttatt gcaaatggaa agagtatcat tgtacggatt
    11701 tcgtttatta gttatttttg tttttaagga aaagctgtgt ccgatattct tttggagaaa
    11761 tcccttgttt tttcttaaat aaataactga aatagttagg gtcattataa ccaatttcaa
    11821 aagcaatatc tgaaatacga tagtctgatt gctgcaataa tttttttgct aattcgattc
    11881 tttgttctgt cagataatcg atgaatgttt ggttaaacga ttgagaaaaa atcgtactga
    11941 aatgagaggg acttaatgca acttgttgag cgacccaagt taaggacata tctggatcgg
    12001 taaaattttc tttgataaaa tccaatgcat ggttcaaaat tggctgatat ttgttccttg
    12061 aaggctgtga gggcagagca gttaagaaag aaatgaacat ataaactgtc gaacggtaaa
    12121 gcgagtcgtc ggcgctgatt gctaagcgtt gactggtgga tgtgcaatga accatttctt
    12181 gcgggatttt cttttctttc aggcagacat gctggagtag cttagtcaag acaaataaac
    12241 gatataatcg tgttcgagtt ggtgtgcctt gtggtttcgt caagtgttcg atgtacttag
    12301 atacttcgtt tttcgataat gaagacaaat caagtgagag gtcaaagtaa aagacagaat
    12361 caactggaag tttcgcttct tgaatcaaat cctcatagct gataatttgt tctttaaggg
    12421 aactggcggt ttcaagtatc atctgcgtga tctcaaaaga tgcaggtaat tcgctgattc
    12481 tttctgcgac aggaccaatg gcgacagaaa aattattttt ttgtcccttt tctaattcat
    12541 ggaccaacgt ttgggctacc tgataactgg tctcgatcac attttcttca agagaatcaa
    12601 ctattaaaaa tttgatgaat cgagaggaaa cagcagagaa gatcacttgt tgattctccc
    12661 taaataaaaa cttcaaataa tcactgagat gagcgtaatc ttcaaagtct gtactgactt
    12721 ggtttgctgc taataatacg gtcatttttt tccctgctaa agagcgattg aaagattcag
    12781 caagtttcaa tgtttcactg atcgtaagtg agcctttgaa caaaccgttc aagaaatgat
    12841 tttttttcaa ttcgaaaaca agatcagaag aagtgtgcgt ttctttttgc tgcaaatcta
    12901 aagcagttac tgctttttct attgcttgtt tcaattcgct acttttaatc ggtttaagca
    12961 gataatcatc tgcctgtacc tggattgctc ctttcatata atcgaaatcg tcgaatccac
    13021 taataaatat gagacgagtc caaggaagta gtttccttgc ttcttttgaa aaagtcaacc
    13081 cgtccatgaa aggcatttgg atatcagtca gaatgatgtc gggctgtaag tcgataattg
    13141 aagacagtgc catctcacca tctccagctt cgccgacata agtgattgga taataagaac
    13201 caaggacagt tatttgttcc cgcaagttat tgcgaatgag gtattcgtct tcgatgataa
    13261 aaactttata catgtttgtt tcctccaatt gttttgggag atagagggat catcagtgtg
    13321 acagttgttc cttctcggta tttgctgtca atcgtgatag gtttttgatc atttccgtaa
    13381 taaagaagga gacgcttgtt tacgttgtaa agtccgtagc cgatactgaa atctgaatcg
    13441 atccctttgg ctaactcttt acgtatttct gccagttttt ctgcagggat gccaatcccg
    13501 ttgtctataa ctttgaattg gatcatctca tcaattttgc atgtggtaat acgaataaat
    13561 cctttgtgac ggacgtactt cgttccatgg tatacagcat tttcaatcaa tggctgcaag
    13621 atcattttta aaatagaaac aggaaaaagt gtttctggta tatcgatctt atagtcaagc
    13681 atatggccgt atcttatttt taagatgact agataatcat tgatatggct aatttctttt
    13741 tcaagaggaa tccagtcttg tcctttgctt aaagacgtac gaaaaaagcc ggaaagtgca
    13801 tgggtcatag tcagagcttc ttcaaagcag tcttgttcga tcaaagaaat aatggcatcc
    13861 aaactgttat aaataaaatg gggagtaatc tgagcttgca atacacgaac ttcactttgt
    13921 gctaaatggt attgtttcaa agcgttctcc cgtaaagact ggtttagatc ttccgccatt
    13981 ttattcatgc tagcggtaag tgtttgtaat tccagtgttt ccggcagggt taaacgaaag
    14041 ccaaggttcc ctgaagaaag ttgttctgac atctcgatca gctgattgat cggttcttgg
    14101 acctttctct ctaaaaagcg acgattcttt ttcgcataga aaaaaatggt actaagaact
    14161 ccaagttcca ctattgttaa tagaatgagc gagcggacaa gtgtcgtatt ttttttgctt
    14221 gctaagtcga tctccacacg aacaaaatct tgtaagatat ctgacagcag ttgagtgaca
    14281 gaatctacct gattgatgat ctcttcattg gtatccataa aatcttttgc caagatatta
    14341 ttgatcagct cgttttgata atcttccagt gtatcgatcg tacgtagcga tacatctaac
    14401 gtactaattt ctttaagtgt atttgtatta tttttgatct ctcgaatatc attcctaagt
    14461 tcttctagga tattttcctc attgaactca tctacggtta cttggccaga aatcaagtcc
    14521 cacatacttt ccaaaacttt ttgatcaacc ttcaaagcaa ttgcatctgc tttttggata
    14581 tttgcgatcg tctgttgata aatcaaaagc tgttgcgaat ataacagtat gctcagtagc
    14641 aaagggataa cagcaaatag aagcatggct ttgtttgctt gggcaaaaat ctgttttatg
    14701 cttaaatttt ttgtcatgat acttctccag tcaataattt ctggttggga tgttagctat
    14761 attctgctgg gaaaagacag tctcggaaat aaaaatatct ttaggtaatg tttttcctgt
    14821 ttgaatattt ccaaaataac gagtaacggt attggctaca taccagccga catcaggttt
    14881 acactcgact acacaattga cgattcctga gcgtagcttc tcgatcatat cttcttgtcc
    14941 atcaatagtt acaatgatca aatcttctcc aggtttgatt gatgtttctt ttatcgcagc
    15001 taaagcaccg tgagtcattt catcactatg gctgtataat acatcgattt cggataaatc
    15061 atttgtttct aaatatcgtt tcatctggtc ttttcctttc atccgaataa aatcaccgac
    15121 aattcggtga acaatagtca tcggaggttt atggtcgatg gttccatctt tttttagggt
    15181 tggtttacga ttgattgatt ccaaaaatcc ttcggagcgt agcttggtcg gtgtggagtt
    15241 ctctgaacca gcaagttcta ggattctgat ttcctgtttc tttgaatttg tgaagtgatt
    15301 gctgacaaat aaacccgccc gatttccttc tgctttgaaa ctggggccaa tgtgggtgac
    15361 gaaaagctcg ggatcattcg tttgaatatg tctgtcgaca agtatgacag ggatccctgc
    15421 tcgctttgct tcttttaata cagagtccca cccggattct tgcaaaggag taaaaatgat
    15481 catatccact ttataggcaa tgaacgtccg tatatcttgg atttgccgtt cctgattcat
    15541 gtagccattt cggtagagaa cttggtaatc ttctttttca agcgcagtga taattgattc
    15601 agtatgcttt ttccgccagc tactctccgt tcctgattgc gaaaaaccaa cgactagtgg
    15661 gtcaggtgct tcctttgtat taggtgtaca ccccatcatt tggtaagcga ttacaacaag
    15721 ggctaaaaaa aataaaacac gtttcacttt aaatcactcc atctggattt gttagtacta
    15781 tcttatcaga taaaagggaa aatcaaagtc aaaaggaatc aatcgaaaaa aatccaagaa
    15841 acttaaaaaa atccaagaca aaaaaagtac acctaaaaaa tttaaagtaa atcccataaa
    15901 aaaatcaatg ttaacgcttt ctttattagc tatactcggt gtatcaattg aaatggggga
    15961 taaaaaatga aaaacaaatt tagaaagatt gttgggacta tcgctatttt gaccgcaagc
    16021 attctattaa gtgcgtgtgg caattcagga tctgcagatg atagtacggg gtatgtagga
    16081 atcgctatgc cgaccaaatc agcagaaaga tggatcgctg atgggaacaa catggtctcg
    16141 gaattagaaa aactgggtta taaaacagat cttcaatatg gagaagataa agtagaaaat
    16201 caagtagcac aaatagaaaa tatgattaca aaaggggtag atacacttgt gattgcttct
    16261 atcgatggtt cggctttaac tgatgtgtta gctaaagcaa aagaagcaga tatcaaggtg
    16321 atcgcctatg atcgtttact gatgaattca gaaaatgtcg attattacgc gacttttgat
    16381 aattttggag taggtgtatc gcaagcagct tatattgaag agcacttagg attgaaagaa
    16441 ggaaaaggac cattcacgat tgaattattt ggtggctcgc ctgacgacaa caacgcacta
    16501 atcaattaca acggagtgat gtctgtttta caaccatata tggacaacgg tcagttggtc
    16561 gtgccatctg gacagacgag tttcagtcaa atcgctacgc ttcgctggga tggttctacg
    16621 gcgcaagcaa ggatggataa tctgttaagc gcaaattata ctgaccaaac attggatgct
    16681 gttttgtcgc catatgatcc aataagttta ggaatcattt cgtcattaaa aggcgtgggg
    16741 tatgggagtg aaagtaaacc tttacctgtg attactggac aagatgcgac agttgctggc
    16801 gttaaatcga tcattgccgg ggaacaaaca caaacgatct ttaaagacac acgtatctta
    16861 gcaaaaaata cgattgaaat gatcaaagca atcagtgatg gagaagaggt tccggtcaat
    16921 gatactgaaa cttatgataa tggggtgaaa actgtcccta cgtatttagc aaatacagta
    16981 tctgtcgata aagataatta tcaagcagag ctgatcgaca ccgattatta taaagaatcc
    17041 gatctgaaaa attaagagga accaaacaag gaactaaaga gatctaagtc agagagagga
    17101 taagaattat ggcggattgt attttaaaaa tggcagatat caccaaaaaa ttttctggaa
    17161 tccgagcgtt agattgcgtg aatctgatga ttgaacgtgg ggaaatccat gcattgtgcg
    17221 gagaaaacgg agcgggaaaa tcaacattga tgaatgtctt gagcggcttg tatccatatg
    17281 gctcctatga cggggaaatc ttttatgatg gaaagctgtg tcaatttaaa gatttgaaag
    17341 atagtgaaga aaaaggcatc gtcatcattc atcaagaatt agcactaagt ccttatcttt
    17401 caataaaaga gaatattttt ctcggaaatg aacaagcaga aagaggcgtg attgattggg
    17461 aactgacaga aaagaaaaca tatgaactgc tgaataaagt cggtttgtct atcgacccga
    17521 atcttctagt atcccagatt ggcgtagggc aacagcaact agttgaaata gctaaagcat
    17581 tatcaaaatc cgtccgtctg ttgattttgg atgagcctac cgctgcattg aatgaagaag
    17641 aaagcgcgaa tttgctgaga ttgatccgag agttgaaaga gcaaggtgta acctccatca
    17701 ttatttcaca taagctgaat gaaatcgtgg atatcgcaga tcgaatcacc attttacggg
    17761 atggacagac gattgagaca ctagaaaatg aaatgatcag cgaagagcat attattcgcg
    17821 gaatggtggg cagagattta gttgatcgtt atcctgaaag aaaaccggat attggagaag
    17881 tttattttaa agtagaagac tggacggtct atcaccctct tgatcacgag cgaatcataa
    17941 acgaccacat caacttgcat ttgaaaaaag gagagattgt aggaatagct ggactgatgg
    18001 gagcgggcag aactgaattc gctatgagtc tatttggaca ttcttatgga agcaagatat
    18061 ccggacgtat attaaaggaa ggggcagaga tatcagttaa gaccgttcct tcagccattg
    18121 caaacggact cgcttatgtc tcggaggacc gaaaatcttt aggactgaat ctgctgatgg
    18181 atattcggga aaacgtgacg ctgagcagtt tggataaaat aagtcaaaaa ggagtattag
    18241 ataaagaaaa agaagtaact gaggcagaaa atttccggaa aaagatgcgt ataaaaacaa
    18301 actccatcca tcaaattgtc agtagtctta gtggtgggaa tcaacaaaaa gtcgttttat
    18361 ccaagtggct gatgacacaa cccgatattc tttttttgga tgagccaacc cgtgggatag
    18421 atgttggggc aaaatatgag atctacacga ttatcgaaga aatggcagca aatggcaaag
    18481 ctgtctgtct tatctcttct gaacttcctg aaatattagg gatgtgtgat cgaatctata
    18541 cgatgaacga aggacgtatt acaggagaag tctcgcgaga agatgcagat caggaagtct
    18601 tgatgaaatt aatgacgaaa gaagaggtgg gttaagttgg aacaggcaaa aaatcttacg
    18661 gataaaaaag agaaaaaact aaatttgaaa agtagagcag ctgatctttt tggtcgctac
    18721 agcatggtga tcattttagc gggactactt attgcgtttc aattgatgac ggatggtatt
    18781 ttctggcgtc cattgaatat aactaacctt gttctgcaaa atagccatat attagtacta
    18841 gctgcaggga tgttattagt tgtgctctta ggtcatgtgg atttgtcggt agggtcagtg
    18901 atggcgtttg ttggtgcaat tgcgggcatg atgatggtca atcgccaaat gaacccgtgg
    18961 atgacagtta tcgcatgtct tgcagtaggc gggctgatcg gcgcatggca aggtttttgg
    19021 gtagcttatg cagggatacc ggcgtttatc gtgactttag caggattgct gatgtttcgt
    19081 ggactgacac aagttgttct aggaggccaa tctctagcac cttttccagc tgttttccaa
    19141 aaaatgtcta cgggttattt accagactta agcgaaggaa gcagccttca cttcttgaca
    19201 ttgattttag gaatgatcat cacgatcgtg ttagttgttg gtcaatggcg aacaagaaat
    19261 cgtcggaaaa atcatttatt tgaagtggaa tctccaacag ctttcttggt aaaggcggta
    19321 tttacagcag tcgttgtact aggaataact tatatctttg cctcttatca agggtatccg
    19381 attattttga tcattcttgg tttgatcgtt gcagcatatg ggtttttgac aaataaaaca
    19441 gtagcaggtc gacagattta tgcgacgggc ggaaatcgaa aagcggctga actatcaggg
    19501 atcaaaacaa agcgaatcac gttctgggta ttcgtcaaca tgggagtcat ggcagcttta
    19561 gctggattag ttttagctgc tcgattgaat gcggcaacac ctcaagcagg tacatcgatg
    19621 gaactggatg caatggcggc agtatatttt ggtggtgctt cgacttccgg cggcattgga
    19681 acaatcatgg gtgcgattgt cggtggcttg gtcatgggtg tattgaacaa tggcatgtcg
    19741 attttaggcg tcggggtaga ctggcagcag gcaatcaaag gattgatctt gcttttagcc
    19801 gttgtgctag acttgtataa taagaaaaaa cgaacaacgt aggagaacgc cggttattca
    19861 gaaaaaagac tgtgacaaga catttgtcac agtctttttt tcgctaaaag cgaacattca
    19921 ttgtttcgtt tgtatagact aaaaataata tatgtttttt taccattcgt ataaattctc
    19981 tttttgctct tgattttttt gcttaattga gttaaattac atagggcgct aaaaaaagcc
    20041 ttgattaaat taataatgtt cgtgttttgc tggatgtgaa catcaggagg ataatgtaat
    20101 gaaagtattt gattacgaag atattcaatt aattcctaat aaatgtatcg taaatagccg
    20161 ttctgaatgt gatacaacgg tcacattagg taaacataca ttcaaaatgc cagtagtgcc
    20221 tgccaacatg cagacgatta ttgatgaaac aatcgcagaa ttcttagcgg aaaatggtta
    20281 tttctatatc atgcatcggt ttgatgaagc agctcgaatt ccgtttatca aaaaaatgaa
    20341 aaagcgtgga ttgattacct cgatcagtgt tggcgtcaag aaagaggaat attcctttat
    20401 agagaagttg gcagaagaat cgctaaatcc tgattatatc actatcgaca tcgcgcatgg
    20461 acatgctaac tcagttattg atatgattca gcatattaaa aaatatttgc ctgaaacttt
    20521 tgtgattgcc ggtaatgttg gtacaccgga agccgtccgt gaattggaaa atgcgggcgc
    20581 agatgcaaca aaagtcggga ttggtccagg aaaagtatgt attacaaaaa tcaagacagg
    20641 ctttgggact ggaggctggc aactggcagc tcttagatgg tgtgcgaaag cagctcgcaa
    20701 gccgatcatc gctgacggtg ggatcagaac acatggcgat attgcaaaat ctgtccgttt
    20761 tggtgcgaca atggtcatga ttggttcatt gtttgccggt cacgaggaat ctcctggcga
    20821 aacaaaagtc gaaaatggaa tcgtgtataa agaatacttt ggttctgctt cagaattcca
    20881 aaaaggcgaa aaacgaaatg ttgaaggaaa gaaaatctgg atccaacaca aaggatcttt
    20941 gaaagataca ctagtggaaa tgcaacaaga ccttcaatca tctatttctt atgctggagg
    21001 aagagatctg gaagccattc gaaaagttga ctatgtcatc gtgaaaaatt cgatttttaa
    21061 tggagacaca atctaaaaaa taaataaaat attaggatgt tgtgaaaaag ttgtcctgca
    21121 ttaagtaata agaacagcca aacaaaaata gttctttata tgttttgttt ggctggaatt
    21181 attatcgcag atacaagctt ttgaacatca ttattcagaa taagggactg tgataagatt
    21241 tttgtcacag tcccttatgt tttgcaaaaa aagtttttct tttttctcgt aaattcggta
    21301 aaatcaaaag aaaaaataga caatagaaaa gagaagctta tgtgggaaaa agtagagaaa
    21361 aaacaaggac cgatttatcg tcaaattatg gaacagatta tgagagaaat cgaaaacgga
    21421 aggatcaatc ctggaagtca acttgaatca gaacgaaaac tagggcaact ttttggtgtg
    21481 aatcgttcca ccgttgtcca tgcattggaa gagcttaggg agcttggggt actaacgagc
    21541 aaacgtggca gcggatggtt tgttaatcag actgaatggg ggaaattcgc tgttccaaga
    21601 atcgattggc ggcaaatgat ctctcctcgt tatgagcaga cggactggta tgagcaaaaa
    21661 ataagagaag caaagcagat ccaaaaaaca tcgtttcttg atttatacgc tagcgagatg
    21721 ccagatgagc ttttgccgaa ttttgagttt ccttcttatt ctcttgaaga aatcttagcg
    21781 gaagaaaaag aaatggatct tttaggctat actccgttac gtaacaaaat caaacggtat
    21841 ttggaaagta aatttgaatt ggcattttcc cccaaccagc ttcttgtgac tggtggcggt
    21901 cagcaagcaa tctttttgat tttacaaacg attctttcag ctggagaggg gatagctgta
    21961 gagtcacctt ctttttttta ccgattacct cttttcaaag cttcaggaac gcgtttattt
    22021 gggataccga tggatgaaga agggatcgac ttagaaaaac tgaaagaatc gatccataaa
    22081 aataaattga aagcagtgct tatcaacccg aattttcaaa accctacagg aaaagtgatg
    22141 agtcaaaaac gacgagaaaa gctagtagaa ttgtgtcgac agtatcggct tccaattatt
    22201 gaagacgatg tatttacgga tctttcattt ctaaaccaag aagaagtcct tcctatacga
    22261 acgattgatc cggataatgt gttatacgtc ggttcgttat ctagggtttt aggaaaaaca
    22321 acgaaaatcg gttgggtgat aggacctgag acttttatct cgcaactagc tcaagcgcaa
    22381 gaaatgatgg agttttcgat gaatatcctt acacaaatca cagtggctaa tgtatttaat
    22441 gaagcaattg atttgaaaat gcaagatgta agaaaaaagt tgaaagaaaa aagccgactg
    22501 ataagcgctt ggggagattc acaatcgttt ttcaagcttc attcaatttt aggtggttac
    22561 tatgcctgga ttacttggga tgggaaaaaa ctagaccccg aactagcaga aaaagtagta
    22621 gatatgggtc ttggaattgc accaagtcta ctttttgggc aagatataaa tggaatcaga
    22681 atcaattaca gtaggctgga tgaaaaccaa ataccgttgt tttattcaaa gatgaaaatt
    22741 ctagaaaagt ggttaacaga ataatttcac aaagtaaata gattaaagaa tataacagct
    22801 tggatttaaa tttcaagctg tttctttttt ttcggaaaaa gaatacgata taaagaaact
    22861 aagaaaaaac cattatttgt acgtccgttc acgatgtgta aataaaataa atgtttgcaa
    22921 caaaactgta taacaccaac gtttttaaat agaaacagat ggttgtaagt ttgattagaa
    22981 aatagaaaaa atgcagatga aatataaaac gctttcctta ttgtgatttt attcactaat
    23041 gctgttaata tgcggattgg cataatttcc tttcatcgtg aaaatttgtg cttttagctt
    23101 gtatttctca taaatccatg ataatttatt aatgaagacg gttaaatagt gatacagttt
    23161 tattcactgt ttcaatcgca gaatgaagag attttcttat agatgagaga ggaatgtgtg
    23221 acaaatggca aacaaaaaag caccgatcga cttccaagca ctgctagatg aagtcaatgc
    23281 agatttccca gtatatcaag cacttgatca agatggaaac gtagttaaca aagatttagt
    23341 acctgattta tccgatgatg aactcgttga attaatgact catatggtat ggtctcgtgt
    23401 attagaccaa cgttcaacag cgctgaaccg ccaaggacgc ctaggctttt ttgcaccaac
    23461 tgctggacaa gaggcaagtc agctggctag tgcttacgca tttgataaag aagatgttct
    23521 tcttccaggt tatcgggatg tacctcaact agtaaaacat ggtttacctt tatctcaagc
    23581 ttttttatgg tctcgaggac atgctgcagg taattcttat ccggaagaat tgaaagctct
    23641 accaccacaa attatcatcg gtgcacaata tgtacaagca gccggtgttg cattaggttt
    23701 gaagaaacga aacaagaaaa atgtcgtctt cacttatact ggtgatggcg gttcatcaca
    23761 aggtgatttt tacgaagcaa tcaatttcgc tggcgcttac catgcaaatg ccgtatttta
    23821 tatccaaaac aacgggtatg cgatctctac acctcgtgaa aaacaaactg cagcaaaaac
    23881 acttgcacaa aaagctgtcg cagctgggat tcctggtatt caagtcgatg gaatggatcc
    23941 gcttgcagta tatactgtat ctaaaatggc acgggaatgg gcgatcagtg gaaatggacc
    24001 agtcctaatc gaaacactga cttatcgtta tggaccacat acactatctg gtgacgatcc
    24061 aacccgctac cgtacaaaag acacagatga cgattggcag aaaaaagatc cattgatccg
    24121 cttccgtaaa tatttgacgg aaaaaggact ttggtctgaa gaaaaagaag aacaagtcat
    24181 cgaagcaact aaagaagaaa tcaaagcagc tattgctgaa gccgacaaag taccaaaaca
    24241 aaaggtttca gatttcttga aaaacatgtt cgaagtatct ccacaatcaa tcaaagaaca
    24301 aattgcaatt tatgaagcga aggagtcgaa ataatcatgg cacaaaaaac aatgatccaa
    24361 gcaatcacag atgccttagc acttgaacta gaaaatgacg agaatgtcgt tgtttttggt
    24421 gaagatgttg ggaaaaacgg aggagttttc cgggcaactg aaggattgca ggaaaagttc
    24481 ggagaagacc gagtattcga tactccttta gctgaatctg gaatcgcagg attgagtttc
    24541 ggtcttgcgt tagaaggatt tcgtccagtt cctgaaatcc aattctttgg ttttattttc
    24601 gaagcaatgg acgaagtagt agctcaaatg gctcgtacaa gatatcgaat gagcggcact
    24661 agaaatctac ccatcacaat tcgttcgcca tttggtggcg gtgttcatac accagaattg
    24721 cattcggata atttagaagg tttgatcgct cagtctcctg gtattcgagt agtgattcct
    24781 tccaaccctt atgatgcaaa aggactattg atttcttcta ttagaagtaa cgatccggtt
    24841 gttttcctag aacacatgaa actatatcgc tcattccgag aagaagtgcc ggatgaagct
    24901 tacgaagttc ctttggataa agcagcagtt actcgtgagg gaacagatgt atctattatc
    24961 acatacggag caatggtgcg tgaagcaatc aaagcagcag acaatcttgc aaaagaaaat
    25021 atatctgttg aaatcattga tcttcgtaca gttgcgccat tagatgtaga aacgatcatt
    25081 caatccgtgg aaaaaactgg tcgcgtagtt gttgtccaag aagcacaacg acaagcgggc
    25141 gttgccgcgc aagtagtttc tgagatctca gaacgcgcta tcctttcatt agaagcccca
    25201 atcggacgag tttctgcacc tgatacagtc ttcccatttg gacaagcaga aaatgtctgg
    25261 ttgccaaatg caaaagatat cgaagacaaa gtaaaagaaa tcgcggaatt ttaagaaaag
    25321 aagggacgaa acaaagtcat ggcttatcaa tttaaatt
//
LOCUS       DACEUZ010000029        25349 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.029, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000029 DACEUZ010000000
VERSION     DACEUZ010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25349
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.029"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(79..765)
                     /gene="gpmA"
                     /locus_tag="IXG44_001004"
     CDS             complement(79..765)
                     /gene="gpmA"
                     /locus_tag="IXG44_001004"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAQ1657737.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(937..1518)
                     /locus_tag="IXG44_001005"
     CDS             complement(937..1518)
                     /locus_tag="IXG44_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1657738.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(1651..2334)
                     /gene="rpiA"
                     /locus_tag="IXG44_001006"
     CDS             complement(1651..2334)
                     /gene="rpiA"
                     /locus_tag="IXG44_001006"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HAQ1657739.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            2653..3066
                     /gene="rpsL"
                     /locus_tag="IXG44_001007"
     CDS             2653..3066
                     /gene="rpsL"
                     /locus_tag="IXG44_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007085295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HAQ1657740.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            3150..3620
                     /gene="rpsG"
                     /locus_tag="IXG44_001008"
     CDS             3150..3620
                     /gene="rpsG"
                     /locus_tag="IXG44_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HAQ1657741.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            3714..5801
                     /gene="fusA"
                     /locus_tag="IXG44_001009"
     CDS             3714..5801
                     /gene="fusA"
                     /locus_tag="IXG44_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAQ1657742.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            5973..7160
                     /gene="tuf"
                     /locus_tag="IXG44_001010"
     CDS             5973..7160
                     /gene="tuf"
                     /locus_tag="IXG44_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAQ1657743.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            7567..7875
                     /gene="rpsJ"
                     /locus_tag="IXG44_001011"
     CDS             7567..7875
                     /gene="rpsJ"
                     /locus_tag="IXG44_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HAQ1657744.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            7906..8535
                     /gene="rplC"
                     /locus_tag="IXG44_001012"
     CDS             7906..8535
                     /gene="rplC"
                     /locus_tag="IXG44_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HAQ1657745.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            8563..9186
                     /gene="rplD"
                     /locus_tag="IXG44_001013"
     CDS             8563..9186
                     /gene="rplD"
                     /locus_tag="IXG44_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HAQ1657746.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            9186..9476
                     /gene="rplW"
                     /locus_tag="IXG44_001014"
     CDS             9186..9476
                     /gene="rplW"
                     /locus_tag="IXG44_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HAQ1657747.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            9515..10348
                     /gene="rplB"
                     /locus_tag="IXG44_001015"
     CDS             9515..10348
                     /gene="rplB"
                     /locus_tag="IXG44_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010747214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HAQ1657748.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            10391..10669
                     /gene="rpsS"
                     /locus_tag="IXG44_001016"
     CDS             10391..10669
                     /gene="rpsS"
                     /locus_tag="IXG44_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HAQ1657749.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            10692..11039
                     /gene="rplV"
                     /locus_tag="IXG44_001017"
     CDS             10692..11039
                     /gene="rplV"
                     /locus_tag="IXG44_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HAQ1657750.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            11053..11709
                     /gene="rpsC"
                     /locus_tag="IXG44_001018"
     CDS             11053..11709
                     /gene="rpsC"
                     /locus_tag="IXG44_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HAQ1657751.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            11712..12146
                     /gene="rplP"
                     /locus_tag="IXG44_001019"
     CDS             11712..12146
                     /gene="rplP"
                     /locus_tag="IXG44_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HAQ1657752.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            12136..12324
                     /gene="rpmC"
                     /locus_tag="IXG44_001020"
     CDS             12136..12324
                     /gene="rpmC"
                     /locus_tag="IXG44_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HAQ1657753.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            12349..12615
                     /gene="rpsQ"
                     /locus_tag="IXG44_001021"
     CDS             12349..12615
                     /gene="rpsQ"
                     /locus_tag="IXG44_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HAQ1657754.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            12673..13041
                     /gene="rplN"
                     /locus_tag="IXG44_001022"
     CDS             12673..13041
                     /gene="rplN"
                     /locus_tag="IXG44_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HAQ1657755.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            13078..13386
                     /gene="rplX"
                     /locus_tag="IXG44_001023"
     CDS             13078..13386
                     /gene="rplX"
                     /locus_tag="IXG44_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HAQ1657756.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            13414..13953
                     /gene="rplE"
                     /locus_tag="IXG44_001024"
     CDS             13414..13953
                     /gene="rplE"
                     /locus_tag="IXG44_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HAQ1657757.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            13972..14157
                     /locus_tag="IXG44_001025"
     CDS             13972..14157
                     /locus_tag="IXG44_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HAQ1657758.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            14193..14591
                     /gene="rpsH"
                     /locus_tag="IXG44_001026"
     CDS             14193..14591
                     /gene="rpsH"
                     /locus_tag="IXG44_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HAQ1657759.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            14623..15159
                     /gene="rplF"
                     /locus_tag="IXG44_001027"
     CDS             14623..15159
                     /gene="rplF"
                     /locus_tag="IXG44_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HAQ1657760.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            15320..15676
                     /gene="rplR"
                     /locus_tag="IXG44_001028"
     CDS             15320..15676
                     /gene="rplR"
                     /locus_tag="IXG44_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HAQ1657761.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            15697..16197
                     /gene="rpsE"
                     /locus_tag="IXG44_001029"
     CDS             15697..16197
                     /gene="rpsE"
                     /locus_tag="IXG44_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HAQ1657762.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            16212..16391
                     /gene="rpmD"
                     /locus_tag="IXG44_001030"
     CDS             16212..16391
                     /gene="rpmD"
                     /locus_tag="IXG44_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HAQ1657763.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            16435..16875
                     /gene="rplO"
                     /locus_tag="IXG44_001031"
     CDS             16435..16875
                     /gene="rplO"
                     /locus_tag="IXG44_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HAQ1657764.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            16875..18170
                     /gene="secY"
                     /locus_tag="IXG44_001032"
     CDS             16875..18170
                     /gene="secY"
                     /locus_tag="IXG44_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HAQ1657765.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            18232..18879
                     /locus_tag="IXG44_001033"
     CDS             18232..18879
                     /locus_tag="IXG44_001033"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HAQ1657766.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            19070..19288
                     /gene="infA"
                     /locus_tag="IXG44_001034"
     CDS             19070..19288
                     /gene="infA"
                     /locus_tag="IXG44_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HAQ1657767.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            19321..19437
                     /gene="rpmJ"
                     /locus_tag="IXG44_001035"
     CDS             19321..19437
                     /gene="rpmJ"
                     /locus_tag="IXG44_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HAQ1657768.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            19455..19820
                     /gene="rpsM"
                     /locus_tag="IXG44_001036"
     CDS             19455..19820
                     /gene="rpsM"
                     /locus_tag="IXG44_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HAQ1657769.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            19848..20237
                     /gene="rpsK"
                     /locus_tag="IXG44_001037"
     CDS             19848..20237
                     /gene="rpsK"
                     /locus_tag="IXG44_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HAQ1657770.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            20317..21255
                     /locus_tag="IXG44_001038"
     CDS             20317..21255
                     /locus_tag="IXG44_001038"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HAQ1657771.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            21283..21663
                     /gene="rplQ"
                     /locus_tag="IXG44_001039"
     CDS             21283..21663
                     /gene="rplQ"
                     /locus_tag="IXG44_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HAQ1657772.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            21935..22774
                     /locus_tag="IXG44_001040"
     CDS             21935..22774
                     /locus_tag="IXG44_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ1657773.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            22750..23619
                     /locus_tag="IXG44_001041"
     CDS             22750..23619
                     /locus_tag="IXG44_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ1657774.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            23609..24406
                     /locus_tag="IXG44_001042"
     CDS             23609..24406
                     /locus_tag="IXG44_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAQ1657775.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            24609..>25349
                     /gene="truA"
                     /locus_tag="IXG44_001043"
     CDS             24609..>25349
                     /gene="truA"
                     /locus_tag="IXG44_001043"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAQ1657776.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKVRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKY"
BASE COUNT         8227 a         4681 c         5459 g         6982 t
ORIGIN      
        1 agttggcagg agaaaaccgt ttatacgaaa aaagggactg tgacaaatcg tgtcacagtc
       61 ccttttttat agtgtttttt ataggtagta ttttttagca actgttaagt catcatttaa
      121 ttcataaaca agtggttgac ctgttggaat ttcaagatcc atgatgtctt cgtcagagat
      181 accttcgata tgtttagcta gggcgcgtaa tgagttacca tgtgcagcta ctaaaacagt
      241 tttgttgtct agtaatgctg gagcaatttc atcttgccag aatggcaaag cgcgttctaa
      301 tgtcactttc aagttttctc caccaggaat atcgcgtttg tctaacattg cgtaacgacg
      361 gtcatttgct gcagaacctt cgtcagtttc ttccattaat ggaggaagag tatcgtaaga
      421 acgacgccag atgtgcactt gttcgtcgcc gtatttttca gctgtttctt ttttgttcaa
      481 accttgtaat ttaccatagt gacgttcgtt taagcgccat gatttgattt gtggtaccca
      541 aagttgatct gaatattcta agatcaagtt acatgttttg attgcacgag ttaaaacaga
      601 agtgtaagca acatcaaatt cgattccagc ttctttgatt ttgcgtccgc cttcttttgc
      661 ttcttcgatt ccttctggtg ctagatttac atcagcccaa ccagtaaatt ggttcaatgc
      721 attccattca ctaagaccgt gacgagaaaa aactaatttt ggcatgttta gctctccttt
      781 taagataggt ttatatttct gaaattttca gttctgtttt attgtacaat tttttggaag
      841 agatttccat cgaaaaatta gataagattc tatgagaaaa caagtagaga atataaaaaa
      901 acgaacagtg cccacgcaac tgttcgtttt taggtattag atgcataatt gctcgatttc
      961 tttttcaggt aaattgatat atttataaca tgttccaact gaaacaccgc attttgaaga
     1021 aataaattgg atcgtttctt ttttttcata atagagacgt cggatttttt tgatcgtttt
     1081 tgcatcgatt tttggtcttc cgccgatttt tcctttttta cgagcttcat ctaatccaac
     1141 taatgtacgt tctttgatca atgcgcactc catgtttgct aggttttcca ttaattggaa
     1201 atagattttt cccatttcgc gcgtatcgat tccttcatca agactaatca agtgcagccc
     1261 gcgatctttg aataattgcg tgagttctgt taattgccgc gtagatttgc ctaatcgatg
     1321 aagttggcaa atgactagtg catcgccttg ttgcatgtca gcgatcactg actccaaatg
     1381 aacagcagta gaatcatttt gttccacttc atatgtctca tggaaaattt cttgacaacc
     1441 aaagtcagat aattgatcaa cctgagttgt taaatcaacg tcatttatgg ttgtgcgtgc
     1501 ataaccaata attttcatgg tgattccttt ctagcagtga ttttctaact ggatataaca
     1561 gtaaaaatgt agcataaagt acgtcaaaaa gatagttttt tcgcaaattt tccgctgtct
     1621 ttttttattg gatgatcaag cagtttttct ttatttacga cggattgcgt caagaatctc
     1681 aacgcctttt tctgagccga caactacttt agaaaccatg tttaggaaaa gcccatgttc
     1741 tactacccca acaagttcat ctaaataggt agctaagctt tctggctgtt cgatgacttc
     1801 tagatgcaga tcaatgatgt aatgaccacc atctgtggtt aagacttctc cgttttcgtc
     1861 tgtacgcaag acgggttgga atcttttttc ttcaaaaata tgaagtagct gttgggagcc
     1921 ataagggatc acttcaactg ggagtgggaa cttcccaagt ttatcgacta atttagaaga
     1981 atcaacgatc caaatcgttt cttttgagta cgtagcgact attttctcga aaagcaaagc
     2041 agcgccgccg cctttgatcc cttgaaaatc agcactgatt tcatcagcac cgtctattgt
     2101 caaatcgact acggggactt catcaatact ctttaacggt attcctaatt tttccgcttg
     2161 ttcttttgtc gcatttgaag tagtaactcc tgtgatacgt aaaccttctt cacgcatacg
     2221 gcgaccaatc tcttctacca tataataggc ggttgaaccg gtcccgagac ctacgatcat
     2281 cccgtccttt acgaatttcg cggcttcaat gccagctaat tgttttaaat tcatctttgc
     2341 gtccaccttt ttttagtttg agcatcgtgc tttcctcact attttagcgt gatttacggt
     2401 aaaaagatac cattcttttt tattccaata aagctttttc aaaaaaaatt gcatctcttg
     2461 ttgacatgtt ttttcattcg tgatattcta acaaaggtgc tttatctaca ggtttctgtg
     2521 aagaaacgtg ctgactgttt atcaaagcat atttgataag gatgcagaca ttgcatttat
     2581 tttttatcac aaaaaaagaa ccacctggat gtgtggaact agacgcaaat cgtgggaagg
     2641 aggaaacaag gaatgcctac aattaaccaa ttagtacgta aacctcgtaa atcaaaggtg
     2701 gaaaaatcta attcacctgc tttgaacaaa ggatataaca gctttaagaa aactcaaact
     2761 aacgtaaact cacctcaaaa acgtggggta gcaactcgtg tgggtacaat gacacctaaa
     2821 aaaccgaact cagctttacg taaatatgca cgtgttcgtt tgtcaaactt aattgaagtt
     2881 actgcttata tcccagggat tggtcataac ttacaagaac acagtgttgt attattacgc
     2941 ggtggtcgtg taaaagactt accaggggta cgttaccata tcgttcgcgg tgctttagat
     3001 actgctggtg ttaacgaccg taaacaaagc cgttctaaat acggaactaa aaaacctaaa
     3061 gcttaatttt aattattaaa caaatgaaac ttgcttctac tatatagaag taattactat
     3121 gaacaatatt tcggaaggag gagttacgga tgccacgtaa aggtcctgtt gcaaaacgtg
     3181 atgttttacc agatcctatt tataactcaa aattagtaac tcgcttgatc aaccgtgtaa
     3241 tggttgacgg aaaacgcggg attgcagcta acattatcta taatgccttt gacttgatca
     3301 aagaatctac aggtaacgat ccattagaag tgtttgaaca agcaatgaaa aacgttcaac
     3361 ctgttcttga agttaaagca cgtcgtgttg ggggttcaaa ctaccaagta ccagttgaag
     3421 ttcgccctga acgccgcgtt actctaggtc ttcgttgggt agtaaactac gctcgcttgc
     3481 gcggtgaaca cacaatggaa gaacgcttag caaaagaaat catggatgct gcaaacaaca
     3541 caggtgcttc tgttaaaaaa cgtgaagaca ctcataaaat ggctgatgcc aaccgtgcgt
     3601 ttgctcacta tcgctggtaa gattctctct gtttggtcgg cttttataag tcgacctcag
     3661 atagtgtcct tatttttagc aactagatag taaagagagg agaaacaagg agaatgacca
     3721 gagaattttc tttacaaaac actcgtaata tcggtatcat ggcccacatc gatgctggta
     3781 aaacgactgc tactgaacgt atcttgtact atactggtaa aatccataaa attggtgaaa
     3841 ctcatgaagg agcttcacaa atggactgga tggaacaaga acaagaacgt ggtattacga
     3901 tcacttctgc tgctactaca gcacaatgga agggtcaccg aatcaacatc atcgacacac
     3961 caggacacgt agacttcaca gttgaagttg aacgttcatt gcgtgtactt gatggtgctg
     4021 taactgttct tgatgcacaa tcaggtgttg aacctcaaac tgaaacagtt tggcgtcaag
     4081 caacaactta tggtgtacct cgtatcgtat ttgcaaacaa aatggacaag atcggtgctg
     4141 acttcttata ttcagtatca actttacatg accgtttgca agcaaacgct cacccaatcc
     4201 aattaccaat tggtgcggaa gacgacttca caggaattat cgaccttgta acaatgaagg
     4261 ctgaaatgta tacaaacgat cttggaacag agatcgaaga aacagaaatt cctgaagaat
     4321 accgcgaatt agctgaagaa tggcgcgaaa aattggttga agctgtagct gaaactgatg
     4381 aagaacttac tttgaaatac ttagaaggtg aagaaatcac tgaagctgag ttaaaagaag
     4441 gtatccgccg cgcaacagtt aacgtagaat tctacccagt actatgtggt tcagcattta
     4501 agaacaaagg ggttcaatta ctattggacg ctgttcttga ttacttgcca tcaccgttag
     4561 atatcccagc tatcaaaggg attgacccta aaactgatga agaagtagaa cgtcctgcag
     4621 acgacagcgc tcctttctca gctttagcat ttaaagttat gactgaccca ttcgtaggtc
     4681 gtttaacttt cttccgtgtg tactctggtg ttttacaatc aggttcatac gtacaaaacg
     4741 caacaaaagg caaacgtgaa cgtgttggtc gtatcctaca gatgcatgcc aactctcgtt
     4801 cagaaatctc tgaagtttac gctggagata tcgctgctgc tgttggtttg aaagacacaa
     4861 ctactgggga cacactatgt gatgaaaaga gtctagttat tcttgaatcc atggaattcc
     4921 cagaaccagt tatccaagtc gctattgaac ctaaatcaaa agctgaccaa gataaaatgg
     4981 gtgtggcttt acaaaaacta tctgaagaag atcctacttt ccgtgctgaa acaaacgtgg
     5041 aaacaggcga aacaatcatc gctggtatgg gtgaacttca cttagatatc atcgttgacc
     5101 gtatgcgtcg tgaattcaaa gttgaagcta acgtaggtgc gcctcaagta tcttaccgtg
     5161 aaactttccg tgctggcact caagccgaag gtaagtttgt acgtcagtct ggtggtaaag
     5221 gtcaatacgg tcacgtatgg attgaattta caccaaatga agaaggtgct ggtttcgaat
     5281 ttgaaaatgc gatcgtcggt ggggttgttc ctcgtgaata cattccagct gttgaaactg
     5341 gtttgaaaga tgctatggaa aatggtgtct tagctggcta tccattagta gatatcaaag
     5401 ctaaacttta cgatggttca taccatgatg tcgattcaaa tgaaactgcc ttccgtgttg
     5461 ctgcttctat ggcattacgt gcagcagcta agaaagctaa tccagtaatc cttgaaccaa
     5521 tcatggctgt agaagttgtt atccctgaag attatttagg tgatgttatg ggtcacgtaa
     5581 ctgctcgtcg tggacgtgtt gaaggtatgg aagctcgcgc tggcggccaa caagttgttc
     5641 gtgcgatggt tccacttgct gaaatgtttg gttatgctac aactcttcgt tctgctactc
     5701 aaggtcgtgg tacatttaca atgacatttg accactacga agatgtacca aaatcagtac
     5761 aagaagaaat cattaagaaa aacggcggaa aagctgaata atttcttaaa accggatagt
     5821 cctgcaaaga gattatccaa agagactata tttttaatat agtttcgtgt aaaataatta
     5881 tcgatgatat gggcatgtgg tgtaccttgt gtacatcata tacctatcag acaaataaaa
     5941 aatatattgt atttttagga ggacatttta aaatggcaaa agaaaaattt gaccgttcta
     6001 aaccacacgt taacattggt actatcggac acgttgacca tggtaaaact acattaacag
     6061 ctgcaatcac aactgtacta tctaagaaaa acggcggcca agctatggct tacgatcaaa
     6121 tcgatggtgc tccagaagaa cgcgaacgcg gaatcacaat ctcaacagct cacgttgagt
     6181 atgaaacaga tacacgccac tacgctcacg ttgactgccc aggacacgcg gattacgtta
     6241 aaaacatgat cactggtgct gctcaaatgg acggagctat cttggtagtt tctgctgctg
     6301 acggcccaat gcctcaaact cgtgaacaca tcctattgtc tcgtcaagtt ggtgttcctt
     6361 acatcgttgt attcttgaac aaagtagaca tggttgatga cgaagaatta ctagaattag
     6421 ttgaaatgga agttcgtgac ctattaacag aatacgaatt ccctggtgac gatgttcctg
     6481 tagttgctgg atcagctttg aaagctctag aaggcgacgc ttcatacgaa gaaaaaattc
     6541 ttgaattaat ggctgcagtt gacgaataca tcccaactcc agaacgtgac aacgacaaac
     6601 cattcatgat gccagttgaa gacgtgttct caattactgg acgtggtact gttgctacag
     6661 gtcgtgttga acgtggacaa gttcgcgttg gtgacgaagt tgaagttgtt ggtattgctg
     6721 aagaaacttc aaaaacaaca gttactggtg ttgaaatgtt ccgtaaattg ttagactacg
     6781 ctgaagctgg agacaacatt ggtgctttac tacgtggtgt tgcacgtgaa gacatccaac
     6841 gtggacaagt tttagctaaa ccaggtacaa tcacacctca tacaaaattc tctgcagaag
     6901 tatacgtgtt gacaaaagaa gaaggtggac gtcatactcc attcttcact aactaccgtc
     6961 cacaattcta cttccgtaca actgacgtaa caggtgttgt tgaattacca gaaggaactg
     7021 aaatggttat gcctggcgac aacgtaacaa tggaagttga attaatccac ccaatcgcta
     7081 tcgaaaacgg tactaaattc tcaatccgtg aaggcggacg tacagttggt gccggtgtcg
     7141 ttacagaaat caaagcttaa ttcacttaag ttttaaagta aagcaacatt tattcggacg
     7201 taatcgctcg gaacgaaggt cttaggatct ttgttccgag cttttttatt tattgcagga
     7261 tcaggaaaaa agacaggagt attataaata gaagaaacac gaaataaaag agtcagtatt
     7321 ttctaaatga aactttatga aaacaaaatg ttttttacaa gaaaaagcct tgctttgtga
     7381 gtttatatat attataatga caaaggtgcg tttactttag tgaattgcac agacgtaatc
     7441 agaatgattt cgtcggctaa gaaacgagag gttgcgacac gcccggacgc tttgccacga
     7501 acgagcgtga cggaaatttt cgtggagcta tgtctacttt tcaaaatagg cgaaggaggg
     7561 aacaagatgg caaaacaaaa aattcgtatc cgtttaaaag cgtatgaaca ccgtatttta
     7621 gatcaatcag cggataaaat cgtggaaact gcaaaaagaa ctggagctag cgtgtctggt
     7681 ccaattccat taccaactga acgcagtctt tacacaatca tccgtgcgac tcataaatac
     7741 aaagattcac gcgaacaatt cgaaatgcgt acacacaaac gtcttatcga cattgtgaac
     7801 ccaacaccaa aaactgttga tgctttgatg aagctagact taccatctgg tgtaaacatc
     7861 gaaatcaaat tataaaacta aaacaaaaat aatggaggtg tactcatgac caaaggaatc
     7921 ttagggaaaa aagtgggaat gacacaaatc ttcactgaat ctggtgaatt aattccagta
     7981 actgttgtag aagctactcc aaacgtagta ctacaagtga aaacaatgga aacggatggc
     8041 tacgaagcta ttcaagttgg ttaccaagat atgcgtgaag ttttatcaaa caaacctgcg
     8101 aaaggtcatg ttgcaaaagc aaacacggct cctaagcgct tcattcgtga attcaaaaat
     8161 gttgagctag gagaatatga agtaggaaaa gaaattacag tagatgtttt ccaagcagga
     8221 gacattattg atgtaacagg tactacgaaa ggtaaaggat tccaaggggt tatcaaacgc
     8281 cacggacaaa gccgcggacc aatggctcac ggttctcgtt accatcgtcg tcctgggtca
     8341 atgggtccag ttgcacctaa ccgtgtattt aaaaacaaac gtcttgctgg ccgtatgggt
     8401 ggcaaccgcg taactatcca aaacttggaa gttgtacgtg tcgatgcaga aagaaatgtt
     8461 atcttgatca aaggtaatgt tcctggagct aaaaaatctt taattactat caaatcagct
     8521 gtgaaagcta aataattgta ggaagaaagg aggaactaag gaatgccgaa tgtagcatta
     8581 ttcaaacaag atggaagcca aaacggtgaa atcacactta acgaagaaat cttcggaatt
     8641 gaacctaatg aaagtgttgt ttacgatgca atcgtaatgc aacgtgcgtc attaagacaa
     8701 ggaacacacg cggttaaaaa ccgtagcgct gttcgcggcg gcggacgtaa accatggcgt
     8761 caaaaaggaa caggtcgtgc acgtcaagga tctatccgct caccacaatg gcgtggaggt
     8821 ggcgtagtct ttggaccaac accacgttct tacagctaca aacttcctaa aaaagttcgt
     8881 cgtttagcaa tgaaatctgt attgtcagaa aaagttgctg aaaacaactt ggtagcaatc
     8941 gaaggcttga acttcgacgc accaaaaaca aaagaattca aacaagttct tgctaactta
     9001 tctatcgact ctaaagtatt ggttgttcta gaaaaaggta acgactttgc tgctttatca
     9061 gcacgtaact taccaaacgt ttctgtagta acttctgata atgtgagtgt gctagacgtt
     9121 gtatcaaata caaaagtgtt agcaacacaa actgctctta ctcaaattga ggaggtgctt
     9181 gcataatgaa cttactagac gtaatcaaac gcccagtgat cactgaaaaa tcaatgcttg
     9241 ccatggatga caagaaatac acttttgaag tggacactcg cgcaaacaaa actttagtaa
     9301 aacaagcggt agaagctgct tttgacgtta aagtcaaaaa cgtgaacatc gtgaacgtac
     9361 gtgcgaaatt caaacgtatg ggcaaatatg caggatatac aaaaaaacgt cgcaaagcga
     9421 tcgttacatt gactgaagat tcaaaagaaa tccaattatt cgaagctgct gaataagcac
     9481 taatagataa ctcattaaat taggagggaa aaacgtggcg attaaaaagt acaaacctac
     9541 ctcaaatggt cgtcgtaaca tgacaggttc tgattttgct gaaatcacaa catcaacacc
     9601 tgaaaaatca ttgttacaac cattgaaaaa ccatgctgga cgtaacaaca acggccgcat
     9661 cactgtacgt catcaaggtg gcggacacaa acgccaatat cgcgtgatcg acttcaaacg
     9721 taacaaagat aacgttgttg caactgttca aacgatcgaa tatgatccaa accgttcagc
     9781 taacatcgcg ttagtacact atgaagatgg agtgaaagct tacatcttag cacctaaagg
     9841 actagaagtg ggtatgaaac ttgtttcagg tccagaagca gatatcaaag tcggaaatgc
     9901 tcttccatta gaaaacattc cagttggtac tgttatccac aacattgaaa tgaaacctgg
     9961 taaaggtgga caattgatcc gttctgctgg tacaagtgct caagtacttg gtaaagaagg
    10021 caaatacgta ttgatccgct tgaactctgg tgaagttcgt atgatcttag ctgcatgccg
    10081 tgctacaatc ggttctgtag gtaacgaaca acacgaatta atcaacatcg gtaaagctgg
    10141 acgctctcgt tggatgcgta aacgcccaac tgtacgtggt agcgtaatga accctaatga
    10201 tcacccacac ggtggtggtg aaggtaaagc tcctatcgga cgtaaagcac cagtttctcc
    10261 ttggggtcaa ccagctcttg gttacaaaac tcgtaacaaa aaagctaaat cagacaaact
    10321 tattgttcgt cgtcgcaaaa ataaataatt actttgacca tgtgtcgcac attttgagag
    10381 gaggttcacc atgggtcgta gtttaaagaa aggacctttt gtcgatgagc atttaatgaa
    10441 aaaagttgaa gctcaagcag gggttgaaaa gaaaaaagtt atcaaaactt ggtcacgccg
    10501 ttcaacagtt ttcccaagtt ttgttggata taccatcgca gtttatgatg gacgtaaaca
    10561 tgtaccagtt tacatccaag aagacatggt aggacacaaa ttaggtgaat ttgcacctac
    10621 aagaacttat cgtggacatg ctgctgacga taagaaaacg aaacgctaat ttgaggggag
    10681 gataaatcca aatggcagaa caaatcacat cagctaaagc aactgcaaaa acagttcgca
    10741 cttcacctcg aaaagcccgt ttagtaattg accttatcag aggtaaaagt gtcgcagatg
    10801 caatttcaat cttgaaattc acaccaaaca aatctgctgg aatcattgaa aaagtattga
    10861 tgtcagcagt tgcgaatgca gaaaataact ttgacttaga tgttgaaaac ttggtagtaa
    10921 gcgaagcttt tgttaacgaa ggacctacaa tgaaacgttt ccgtcctcgt gcaaaaggat
    10981 cagcttctcc aatcaacaaa cgtacaagtc atatcacggt agtcgtatca gaaaaataag
    11041 gagggataat cagtgggtca aaaagtacat ccaattggaa tgcgtgtagg catcatccgc
    11101 gactgggatg ctaaatggta tgctgaaaaa gagtatgctg agttcttaca cgaagattta
    11161 agaatccgta aatttatttc aactaaactt gctgatgctg ctgtatcaac agttgaaatc
    11221 gaacgcgcaa caggtcgcgt aaacatttca attcacacag ctaaaccagg tatggttatt
    11281 ggtaaaggcg gttctgaagt cgaaagccta agaaaagaat taaacaaatt aactggtaaa
    11341 agaatccata tcaatatcgt ggaaatcaaa aaaccagatc tagatgcaaa attagtaggt
    11401 gaaggaatcg cacgtcaatt agaaaaccgt gttgctttcc gtcgcgctca aaaacaagcc
    11461 atccaacgct caatgcgtgc tggcgctcaa gggatcaaaa ctcaagtatc tggtcgtcta
    11521 aacggagcag atatcgctcg ttcagaagga tactcagaag gaactgttcc tcttcatact
    11581 ttacgtgcgg acattgatta cgcatgggaa gaagcagata caacatacgg aaaactagga
    11641 gttaaagtgt ggatctatcg tggagaaatt cttccaacta aaaaaaacac tgagaaagga
    11701 gggaaataat catgttagta cctaaacgtg taaaacaccg tcgtgaattc cgcggaaaaa
    11761 tgcgcgggga agctaaaggc ggaaaagaag tagcattcgg tgaatacggt ttgcaagctg
    11821 ttgattcaca ttggatcaca aaccgccaaa tcgaagctgc tcgtatcgca atgactcgtt
    11881 acatgaaacg tggtgggaaa gtatggatta aaattttccc tcacaaatct tatactgcca
    11941 aagcaattgg ggtacgtatg ggttctggta aaggggcacc tgaaggatgg gttgcaccag
    12001 taaaacgtgg taaaatcatg tttgaaatcg caggcgttcc tgaagaagta gctcgtgaag
    12061 cgttacgtct agcttctcac aaattaccaa tgaaaactaa gatcgtaaaa cgtgaggaaa
    12121 tgggtggtga atcgaatgaa ggttaaagaa atcagagaat taaccactgc cgaaatgctt
    12181 gatcaagaaa aacaattaaa agaagaattg tttaatctta gattccaatt agcaacaggt
    12241 caattagaaa acactgcacg tattaaagaa gtacgtaaat cgattgcacg catcaaaact
    12301 gtgttgcgtg aacaagctaa gtaattatgg aaggaggcca ttaagcgtat gactgaagga
    12361 agaaatcaac gcaaagttta tcaaggtcgc gtggtttcag acaaaatgga taaaacaatt
    12421 accgttgctg tcgaaacaaa gaaaaaccac cctatctatg gcaaacgcat gaactattct
    12481 aagaaataca aagtgcatga tgaaaacaac acagcaaaag ttggcgacat cgtgagaatt
    12541 atggaaactc gtccattatc agctacaaaa cgtttccgtt tgttggaagt agtcgaagaa
    12601 gcagttatta tctaataaca cgagattttc ctatatagat tgaatgcaaa atctgaaagg
    12661 aggatacaca acgtgatcca agcagaaagt cgtttaaaag tcgctgataa ctcaggcgca
    12721 cgtgaaattt tgacgatcaa agtccttggt ggatctggtc gtaaaactgc taacatcggt
    12781 gatgtgatcg ttgctacggt taaacaagca acgccaggtg gggttgtcaa aaaaggtgac
    12841 gtcgttaaag ccgttatcgt tcgaacaaaa tcaggagctc gtcgtacaga cggttcatat
    12901 attaaatttg atgaaaatgc tgcagtgatt attcgtgatg ataaaagccc tcgtggaaca
    12961 cgtatctttg ggccagttgc acgtgaacta cgtgaaaaca acttcatgaa gatcgtttca
    13021 ctagcaccag aagttttata atcttgagac acttatcaaa ggaggtgcga aacagttatg
    13081 tttgttaaaa aaggcgataa agttaaagtt atcactggaa aagacaaaaa caaagaaggc
    13141 gttgtactag cagcgttccc gaaaaaagat aaagttattg tcgaaggtgt aaatgtcgtt
    13201 aaaaaacacc aaaaaccttc tcaagctgct cctcaaggtg gaattgttga gatggaagcg
    13261 ccaattcatg tttctaacgt gatggtggtt gactctactg gtgtagctgg ccgagttggc
    13321 tacaaagaag tagacggcaa aaaagtccgt gtttctaaaa aaaccggtga agttttagat
    13381 aaataaaagt ttaaggaagg agggtcttac tgaatgaacc gcctaaaaga aaaatatctt
    13441 aaagaagtaa ctccatcatt gatggaaaaa tttaactata gctctgttat gcaaacacct
    13501 aaagttgaaa aaatcgtcat caacatgggt gttggtgatg cggtatcaaa cgctaaaaac
    13561 ttagataaag cagttgaaga attaactttg atcactggtc aaaaaccaat gattacaaaa
    13621 gctaaaaaat caatcgctgg attccgttta cgtgaaggaa tgccaattgg tgcaaaagtt
    13681 acattgcgcg gagaaagaat gtacgaattc ttagacaaat tggtttctgt ttcactacca
    13741 cgtgtacgtg acttccatgg tgtaagtaaa aaagcctttg acggtcgtgg aaactatact
    13801 ttaggtatta aagaacaatt gatcttccca gaagtagatt atgatttagt agataaagta
    13861 cgtggtatgg acatcgttat tgtaacaaca gcaaactctg atgaagaatc tcgtgagttg
    13921 ttgacacaat taggcatgcc attccaaaaa taattaaagg aggcgaacta cgtggctaaa
    13981 aaatcaatga ttgctaaaaa caaacgtcct gcaaaatttt caacacaagc ttatactcgt
    14041 tgcgaacgtt gcggacgtcc acattcagtt tatcgtaagt tccatctttg ccgtatttgc
    14101 ttccgcgaac ttgcctataa aggtcaaatt cccggcgtga agaaagctag ctggtaaaaa
    14161 gtaaattcgc ataagaagga ggtaaaagat caatggtcat gacagatcca attgcagatt
    14221 ttctaactcg tatccgtaac gcaaacatgg ttaaacatga gtctttagaa gtacctgcat
    14281 caaaaattaa acgtgacatc gctgaaatct tgaaacgtga aggtttcgtt cgtgatgttg
    14341 aatacatcga agatgacaaa caaggcgtga tccgtgtttt cctaaaattt ggtaaaaacg
    14401 gcgaacgtgt tatcacaaac ttaaaacgta tctctaaacc aggtttgcgt gcttatgtca
    14461 aagctgacga agtaccaaaa gtattgaacg gtttaggtat cgcaatcatt tcgacttctg
    14521 aaggtgttgt cactgataaa gaagctcgtg aaaaaaatat cggcggcgaa gttatcgctt
    14581 acgtatggta atcaagtaga atctacaagg aggtgtcttt aagtgagccg tatcggaaat
    14641 aaagtcgttg ttatccctga aggagtaaca gttactcaag acggaaacaa cgttacagtt
    14701 aaaggaccaa aaggtgaatt gactcgtact ttttcagcag atatcaaaat gaatatcgaa
    14761 ggaaacgaag taactttcac tcgtccaaac gacagtaaag aaatgaaaac aattcatgga
    14821 acaactcgtg cgaacttcaa caacatggtt gtaggtgtca gcgaaggttt ccaaaaagcg
    14881 ttagaactaa tcggggttgg gtaccgtgca caattacaag gatcaaaact tgtattgaac
    14941 gttggttact cacatccagt agaaatcaca ccaccagctg gagtaactgt tgaagtacca
    15001 gctaacacac aagtaattgt taaaggtgcg aacaaagaag aagttggcga attagctgca
    15061 aacattcgtg gaactcgtcc tccagaacct tataaaggta aaggtattcg ttatgttggc
    15121 gaatatgtac gccgtaaaga aggtaaaact ggtaaataat cgtttagaaa aattgcatag
    15181 aagccagcag caggaaaaag gcatttggct gaagtttacc ttcaaaaaaa tacattttta
    15241 cttgtgccgc agcttctatt tttcgaaacg tatttactgc ttgaaatagt agtacaataa
    15301 aaataaagag gtgacaattg tgattacaaa accagataag aacaaaacac gtcaaaagag
    15361 acatcgtcgt gtacgtaaca atatctctgg tactgctgag cgcccacgct tgaacgtttt
    15421 tcgttctaac aaaaacatct acgctcaaat tattgatgac gtagcgggtg taacgctagc
    15481 aagtgcctct accttggata aagaaatttc aggtggaaca aaaacagaag cagcacaagc
    15541 tgtcggtaaa ctagttgctg aacgtgcagc tgaaaaaggc attaaagtag tagtctttga
    15601 ccgtggtgga tacctttacc atggccgtgt gcaagcttta gctgaagctg ctcgcgaaaa
    15661 tggactagaa ttttagaaga aggaggaata ccattcatgg tttatatcga tccaaaacat
    15721 ttggaattag aagaccgcgt tgttgcaatt aaccgtgtaa caaaagttgt taaaggtgga
    15781 cgtcgtctac gttttgctgc tttagttgtt gtcggtgaca aaaacggaca cgtagggttt
    15841 ggtacaggta aagctcaaga agttcctgaa gcaatccgta aagcaattga agatgcgaag
    15901 aaaaacttag ttgaagtacc tatggttggt tcaacaatcc cacacgaagt aatcggtgct
    15961 tttagtggtg gtagaatcct aatgaaacct gctgtagaag gttctggggt tgctgctggt
    16021 ggaccagttc gtgccgtatt agaattagct ggggtagcag atatcacttc taaatcttta
    16081 ggctcaaata caccaatcaa cgttgttcgc gcaacagttg aaggtttgaa acaattgaaa
    16141 cgtgcagaag aagttgcaga acttcgcggc aaatcagttg aagaattaat cggataagga
    16201 ggacaacaat aatggctgaa ttaaaagtta ctttaaaacg cagtattatc ggacgtcctc
    16261 aaaaccaacg cgatacagtt aaagcgttag gtcttacaaa gattaacagt tcagtggtta
    16321 agccttctaa tgaagcaatt aaaggcatga tcaatactgt ttctcaccta gtggacgttg
    16381 aagaaattta atttagaaaa atcataacta gaacattaag gaggtgccaa acctatgaaa
    16441 cttcatgaat taaaacctgc tgaaggttca cgccaagtac gtaaccgtgt tggtcgtggt
    16501 acttcatctg gtaacggaaa aactgccgga cgcggacaaa aaggacaaaa agctcgttca
    16561 ggcggcggtg tgcgtttagg atttgaaggg ggacaaacac cattgttccg tcgtttacca
    16621 aaacgaggat tcacaaacgt gaaccgtaaa gactatgcag tcattaatct tgatgtattg
    16681 aaccgctttg aagacggagc tgaagtaact cctgttgctt tagttgaagc tggaatcgtg
    16741 aaaaacgaaa aagctggaat caaagtatta gctaatgggg aactaaacaa gaaattaact
    16801 gtgaaagcag caaaattctc taaagcagct caagaagcaa ttgaagcggc tggtggatca
    16861 atcgaggtga tctaatgttt agactattaa aagatgcttt taaagtaaaa gacattagat
    16921 cgaaaatcct atttactgta tttgtgttgt ttgtatttcg cctaggtgca catattactg
    16981 taccaggcgt aaatgctgct tcattgcaaa acctttcttc tttgccattt ttcaacatgt
    17041 tgaacttggt aagtggtagt gcgatgcaaa acttttccat tttctcaatg ggggtttcac
    17101 catacattac tgcttctatc gtcattcagc ttcttcaaat ggacatcgtg cctcgatttg
    17161 ttgaatggtc gaaacaaggg gaagttggtc gtaaaaaact gaatcaagcg actcgatatc
    17221 tgacattagt attggcattt atccaatctg tcggtatcac tgctggattc aatcaatacg
    17281 ctcaattagg tttcgtgaat aatcctaacg tagcaactta cgtaacgatc ggtttgattc
    17341 taacagccgg tacgatgttt gttacttggc taggagaaca gatcacagaa aaaggtatcg
    17401 gaaatggcgt atcaatgatc atttttgcag gtatcatttc tagattgcct gaaagtatca
    17461 gagaaattta tgaagattac tttataaata ttgatccttc agacatatgg aaatctgcta
    17521 tttttgtggc cattttacta gtagcggtct tgttgatcgt gacattcgta acatttttcc
    17581 aacaagctga acgcaagatc ccgatccaat atacgaaacg cgtagctggc gcgcctacaa
    17641 gcagctatct gccgttgaaa gtaaatgcag ctggagttat tccagttatc tttgctagtt
    17701 cgtttattgc tacgccaaat gctgttttac aagcattgag cggtagattc ggttctgaaa
    17761 actggtacaa agtgatgatg gagatcttta actataatac agcacctggt gctacgattt
    17821 atacgattct gatcgttgcc ttcactttct tctacgcctt tgttcaagta aaccctgaga
    17881 aattagcgga aaacttgcaa aagcaaggaa gttatatacc aagcgtacgc cctggtaagg
    17941 ggactgaaga gtatgtctca agattactga tgcgtttaag tacagttggt gcagtcttcc
    18001 ttggtttagt ggcattgctt ccaatcattg ctcaaatggt ttgggatctt ccacaatcaa
    18061 tcggattagg cggtacaagc ttactgatcg ttatcggtgt agctttagaa actgcgaaac
    18121 agttagaagg cttaatgctg aaacgtaaat atgttggatt cattgagtaa gaaagcgtgt
    18181 tgaggtttcc cctcaacaca cgttctcaca catttattag gagggaaaac aatgaacctc
    18241 attttaatgg ggctgccagg cgcaggtaaa ggaacacaag ctgaaaaaat cattgatgct
    18301 tatggtattc cgcacatctc aacaggagat atgttccgcg cagcgatgca aaacgaaaca
    18361 gctcttggcc tggaagcaaa gtcttatatg gataaaggtg cattagttcc tgatgaagta
    18421 acaaatggaa tcgtgaaaga gcgactagct gaaccagata cagagaaagg cttcttatta
    18481 gatggctttc cacgtacgct agatcaagca aaagctctgg acgcaatgct aaaagatttg
    18541 aataaaaaaa ttgatgctgt catcgatatc catgttggtg aagaaatctt ggtcgaacgt
    18601 ctagccggcc gatttatctg cagcaattgt ggagcaactt accataaaat tttcaaccct
    18661 acaaaagttg aagacacatg tgatcgttgc ggtgggcatg aattctatca aagagaagat
    18721 gataaacctg agacggttaa aaatcgtctg gctatcaaca tcaaaaatag tgaaccaatc
    18781 ttggcttatt ataaagaaca aggtttgcta aacactattg atggtgctcg tgaaatcgat
    18841 gctgtatttg cagatgtaca aaaaatcatt gaaaaatagc acgattgccc gtagccgact
    18901 tgcattatgc agttactctt gtcaagttca ataaattatg atagaatatg tcagtccgac
    18961 ttctagagaa tcctctagat gatccatttt gagaattttg aggtgggaac tgcagtttcc
    19021 gccattttat cgtgtgaaaa tgcgaaacaa gtacaaggag gtactgtgcg tggcaaaaga
    19081 agatatgatt gaagtcgaag gtacagtcgt cgaaactttg ccgaatgcaa tgtttaaagt
    19141 tgaactagaa aacggacacc aagttcttgc tactgtttca ggtaaaattc gtatgcatta
    19201 catccgtatc ttacctggtg acaaagtaac ggttgaattg tctccatacg atcttaaccg
    19261 tggccgtatt acttatcgct ttaaataatt gtactccgta aaactacagg aggtattatc
    19321 atgaaagtaa gaccatcagt aaaaccaatg tgcgaacatt gtaaagtaat tcgtcgtaaa
    19381 ggacgcgtta tggtgatttg cccagcaaat ccaaaacata aacaacgtca aggataattg
    19441 gaggtgctat aaatatggct cgtattgcag gagtagatat tcctcgtgat aaacgtgtag
    19501 tagtttcact tacttatatc tatggtattg gtaacactac tgctaaaaag atcttagctg
    19561 atgctggtgt atctgaagat gttcgcgttc gtgaattaac gaatgaacaa acagatgcta
    19621 tccgtgcaga agtagataaa ttaaaagttg aaggggatct tcgtcgtgaa gtgaacttga
    19681 acatcaaacg tttgatggaa atcggttctt accgtggaat ccgtcatcgc cgtggattgc
    19741 ctgttcgtgg acaaaacacg aaaaacaatg cacgtactcg taaaggccca gctcgcgcta
    19801 tcgctggcaa gaaaaaataa ttcttaagtg aaggaggtta aaacttcatg gcagcaaaaa
    19861 aagtgaatcg taaacgccgt gtgaagaaga atatagaagc aggtattgca catatccatt
    19921 ctacattcaa taacactatc atcatgatca ctgatactca cggaaatgca ttagcgtggt
    19981 catcagctgg ttcattaggt ttcaaaggaa gcaaaaaatc aactcctttt gccgctcaaa
    20041 tggcagcaga aactgctaca aaagcagcaa tggaacacgg attaaagact gttgaagtaa
    20101 cagttaaagg acctggttct ggacgtgaag cagcaattcg ttcattacaa gctacaggtt
    20161 tagaagtgac tgcaattcgt gacgtgactc cagttcctca taatggtgcc cgccctccaa
    20221 aacgccgtcg tgtttaatga gtgccgctca ttttgagttt tatcagtaac gtcattgaac
    20281 aagacacttt tcgttttgaa aggggtaaag ataagaatga ttgagttcga aaaaccaaga
    20341 atcgcaaaaa ttgatgaaga aaaagattat ggcaagttca tcgttgaacc tcttgaaaga
    20401 gggtatggga ctactttagg gaattcccta cgtcgtattt tattatcttc tttgccaggt
    20461 gctgccatca ctaatattca aatcgatggt gtactgcatg aattctctac catcaaaggt
    20521 gttagagaag acgtcactca aatcatcttg aacattaaag gcttagcgct gaaaatgtat
    20581 ggccaagaag aaaaaaccct tgaaatcgat attaccggac ctgctacagt aactgctggc
    20641 gatattatcg ttgatagtga tgtagaaatt cttaacaaag atatgtacat ctgtagtgtc
    20701 tctgaaggag ctacgttcca tgctcgctta acagtgaaac ctggacgtgg ttatgttcaa
    20761 gcagatgaaa ataaaaaaga agatatgcca attggcgttc ttccagtcga ttcaatctac
    20821 acacctgttc gtcgtgtgaa ctatcaagtt gagaacacac gtgttggaca ccgtgaagat
    20881 tttgataaat tgacgatgga gatttggaca gatggttcaa ttgaaccgtt ggaagcaatg
    20941 agtttgtctg cgaaaatcat gactgaacat ttggatattt ttgtgaactt aactgatgaa
    21001 gcaaaaaatg ctgaaatcat ggtcgaaaaa gaagaaacac aaaaagaaaa aatgttggaa
    21061 atgacgatcg aagagttaga cttgtctgtt cgctcttaca attgtttgaa acgtgcagga
    21121 atcaatactg tacaagaact tacaaataaa tctgagcctg aaatgatcaa agtccgtaac
    21181 ttaggacgta aatcattaga agaagttaaa gcaaaattgc acgacttagg tttaggttta
    21241 cgtaaagaag attaaaacct tttacgaagg agggaaacca acgtgagtta tcgtaaacta
    21301 ggacgcacat ctagccaacg taaagcgatg ttgcgtgatt taacaactga tttattaatc
    21361 aacgaacgta ttgtgactac tgaagctcgt gcgaaagaag ttcgctcaac tgcagaaaaa
    21421 atgatcactt taggtaaacg cggagatttg catgcacgtc gtcaagctgc ggctttcgta
    21481 cgtaatgaag ttgccagcgt gcgtgaagaa aatgaggaca tcgtcattga atcagcttta
    21541 caaaaactgt tcaacgatat tgcacctcgt tacgcagaac gtcaaggcgg atacacacgc
    21601 attttgaaga cagaaccaag acgcggtgat ggtgcaccaa tggttgtctt agaacttgtc
    21661 taaaatcaca ctaataacat cactttatag atgattcttt tagagtgtta tgatgttgga
    21721 agatattttt ccgagtctag ctcagcgctg ctcctcaagc gaatatcgct tggggagtaa
    21781 gcactcatca taaagtgttt ttttatacct tcaattacct gtcatgacag atatgtttga
    21841 aggcataaaa aaacatttta agcgagaaat aattgaaaat tttgtctttt tttgatagtg
    21901 ttagtaggaa ctttaggatg aagaaggagt aatcatggag ccaattattg aattagaaaa
    21961 gataaattat aaataccagc cagatgatct tcgccccgca ttgaaagatg tctcctttac
    22021 aattgataaa ggtgagtgga tcgctattat tggacacaat ggttcaggaa aatcgacact
    22081 tgccaaaacg atcaatggat tgcttctgcc agaatcaggg atcgtgaaag tgggaaatca
    22141 aatattggat gaagaaaata tttggacgat ccgacagatg gttggaatgg tttttcaaaa
    22201 tccagacaat cagtttgttg gttcgacagt agaagatgac gttgcttttg gtcttgaaaa
    22261 tcaagggatt cccagagaag aaatgctagt gcgtgtgaaa gatgcacttg agaaagttag
    22321 aatggctgag tttgcttcac gcgagcctgc tcgtttatca ggagggcaaa aacaacgtgt
    22381 tgccattgcc ggcgtggttg ccttacgacc tgatatcatt attttagatg aagcaaccag
    22441 tatgctagat ccagaaggtc gtgaagaagt gatctctacg atcaagaaga tcaaagaaga
    22501 aagtcaattg accgttatat ctatcactca tgatattgat gaagcagcta atgccaatcg
    22561 gatactagtg atgagacaag gcgaacttgt ccgtgaaggt acaccaaaag aaattttttc
    22621 tgctggtcct gaactgatcg acttaggtct agatctacca tttcctgaaa aactgaagag
    22681 cgcactgaaa gaacgtgggg ttgatgtgcc cagtgaatat atgacagaag aaaggatggt
    22741 ggattggtta tggacatccg ttttgaacaa gtagacttca cttatcagcc gaatacacca
    22801 tttgaacaaa gagccttatt tgacatcaat atgacgatca aagaaaacag ttatactgca
    22861 ttagtagggc ataccggaag cgggaaatca accttacttc agcatttgaa tgcgcttgtt
    22921 aagccaacaa gcggtacagt ccatattgga gaacgagata ttcagccgga tacggataat
    22981 aagaatttaa agcccatccg aaaaaaagtt ggcatcgtct ttcagtttcc agaagcacag
    23041 ctattcgaag aaacggtagc aaaagacatt gctttcggtc ctaaaaactt tggtgtcagc
    23101 gaagaagaag cactagtcct agcaaaagaa acattagaac aagttgggct ggatgaaagc
    23161 tatttggaac gttcgccatt tgaactttca ggagggcaaa tgcgtagagt agcaatcgct
    23221 ggtgtgcttg ccatgaggcc ggaagtactt gtattggatg aaccaacggc aggacttgat
    23281 ccccagggta gaaaagaaat gatggagatg ttttggcgtc ttcataagga acagcagatc
    23341 actattgtat tagttacgca tttaatggat gatgtagcca attttgcaga ttatgtctat
    23401 gtgttggaaa agggacgaat cgttaatagc ggggagccgc aagaggtctt tcaaaacatt
    23461 gaatggctaa aagaaaaaca actaggcgta cctactgcca ccgagtttgc agaagaatta
    23521 atggctaaag ggatgaactt tgctaccttg cctttgacag ctgaagaatt agcagacgct
    23581 atcgtccatt atgctggagg aaatgcccat gatgaataaa ttgatatttg gtcgttatat
    23641 gcctggagat tccttgatcc atcgattgga tccccgagct aaattattag ccagttttta
    23701 ttttatcgga atcatttttt tagcaaataa ttgggaaacg taccttttgt tggcattttt
    23761 cacattactt tctgtcttct tgtcaaaagt agatttgaaa ttttttatcc gaggcattcg
    23821 cccattgatc tggctaatcc tcttcactgt agctttgcag gtcttgttta caagtggcgg
    23881 agaaacttat tggagttggg gtgttttcca tatcactgaa tttggtgtga tgaacggcct
    23941 ctttattttc tgtcgattcg tgttgatcat ttttatgtcg actctactca cgcttactac
    24001 accgcctttg gaattatcgg atgcgattga gtacttgctt cgtccattga aagccgtacg
    24061 gttcccagtc cacgaaattt cattgatgct gtcgattgct cttcgttttg tgcctacatt
    24121 gatggacgaa acagaaaaga tcatgaacgc tcagcgcgct cgcggtgttg attttggtga
    24181 aggaaaccta gttcagaaaa tgaaagcagt cattcctttg ctgattcctt tatttgtcag
    24241 cagcttcaac cgtgcggaag atcttgcgac tgctatggaa gcaagaggct acaaaggtgg
    24301 ggaaggaaga acgaaatatc gtgtattgca ttggcataat agagacacga tggttatcat
    24361 tgcttttgta ttattaactg tgctactgat ctttttgaga ggataaacgt tcagtgggcc
    24421 gctatctatt cttttcttgc tgatctgttt gcaagaaagg atgcgagcag tgtatgacaa
    24481 gaaatattag ggtctgtggc agactaaggt cacaggcctc tttcttttat ctgaaaagaa
    24541 agaacgaaaa aattttataa aaaaatcatt attccagata accttttagg aacaaaggag
    24601 agaaaaacat ggtacgttac aaagcaatca ttgcatacga cggaacaaat tttaatggat
    24661 tccaaaaaca gccaaatggg cgtactgttc aagaagaagt agaaaaaaca ttacaaaaaa
    24721 tggcaaatgg aaaagaaatc acggtctttg gttcgggaag gacagatgcc ggagtacatg
    24781 cgattgggca agtaatccat tttgattatc cagaggagcg tccgttagaa cgaatgcgtt
    24841 tcgcgctaga tacccagtca ccagaggata ttgctgtaag acaagtagaa attgtttcag
    24901 aagacttcca cgcaagatat ttggtcaaag aaaagacgta tcaatttcga gtggatatag
    24961 ggaaaccacg cagtcctttt cgacgccatt atgcaagtta ttttccttac cctcttgatt
    25021 tggaaaaaat tcagcgggcg cttcctgatt tattagggac tcacgatttc acctcttttt
    25081 gtgcatcagg aagctcgata gaggataaag tacgtacgat ctacgaggct aagatggagg
    25141 taaatgaaac aaaagacgaa ttgttgttta catttagggg aaatggcttc ttgtataaga
    25201 tgatacggat cttagtagga acgcttttga aaattggcaa tggccgattg cctgaagata
    25261 gtatcccaga tattattgcg aaaaaagatc gtaatgcagc aggacctaca gcacatcctg
    25321 aaggacttta tttgtatgaa gtgaaatat
//
LOCUS       DACEUZ010000030        24509 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.030, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000030 DACEUZ010000000
VERSION     DACEUZ010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.030"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            <1..3417
                     /gene="rpoB"
                     /locus_tag="IXG44_001044"
     CDS             <1..3417
                     /gene="rpoB"
                     /locus_tag="IXG44_001044"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAQ1657777.1"
                     /translation="DYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRETGEIKAQEV
                     FFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGSTVIPNRGAW
                     LEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLRNTVEKDLHK
                     NASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVGRYKVNKKLD
                     LKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSVTYYPSEDGV
                     VTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYFLNLMEGIGN
                     VDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQQLINIRPVV
                     ASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVRDVHYSHYGR
                     MCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQIDYLTADIEDH
                     YIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVAVATACIPFL
                     ENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKHDGVVEYVDA
                     SEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLADGPSMEQGEM
                     ALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDTKLGPEEITR
                     EIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERLLHAIFGEKA
                     REVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRKIHEGDKMAG
                     RHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHLGMAARQLGI
                     HVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGVMYMIKLAHM
                     VDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTLQEILTYKSD
                     DVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEETEIELRDMDD
                     EDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNLD"
     gene            3496..7149
                     /gene="rpoC"
                     /locus_tag="IXG44_001045"
     CDS             3496..7149
                     /gene="rpoC"
                     /locus_tag="IXG44_001045"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HAQ1657778.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKSVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(7744..8769)
                     /locus_tag="IXG44_001046"
     CDS             complement(7744..8769)
                     /locus_tag="IXG44_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1657779.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            9044..10729
                     /locus_tag="IXG44_001047"
     CDS             9044..10729
                     /locus_tag="IXG44_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HAQ1657780.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            10743..12026
                     /locus_tag="IXG44_001048"
     CDS             10743..12026
                     /locus_tag="IXG44_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAQ1657781.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            12096..12965
                     /locus_tag="IXG44_001049"
     CDS             12096..12965
                     /locus_tag="IXG44_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1657782.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            12962..13798
                     /locus_tag="IXG44_001050"
     CDS             12962..13798
                     /locus_tag="IXG44_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1657783.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            13824..14891
                     /locus_tag="IXG44_001051"
     CDS             13824..14891
                     /locus_tag="IXG44_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HAQ1657784.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            14888..15715
                     /locus_tag="IXG44_001052"
     CDS             14888..15715
                     /locus_tag="IXG44_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAQ1657785.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            15760..16785
                     /locus_tag="IXG44_001053"
     CDS             15760..16785
                     /locus_tag="IXG44_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ1657786.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            16782..18965
                     /locus_tag="IXG44_001054"
     CDS             16782..18965
                     /locus_tag="IXG44_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAQ1657787.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            18965..20095
                     /locus_tag="IXG44_001055"
     CDS             18965..20095
                     /locus_tag="IXG44_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAQ1657788.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            20092..20769
                     /gene="pgmB"
                     /locus_tag="IXG44_001056"
     CDS             20092..20769
                     /gene="pgmB"
                     /locus_tag="IXG44_001056"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAQ1657789.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            20853..21380
                     /gene="msrA"
                     /locus_tag="IXG44_001057"
     CDS             20853..21380
                     /gene="msrA"
                     /locus_tag="IXG44_001057"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ1657790.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(21581..22267)
                     /locus_tag="IXG44_001058"
     CDS             complement(21581..22267)
                     /locus_tag="IXG44_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HAQ1657791.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            22347..23885
                     /locus_tag="IXG44_001059"
     CDS             22347..23885
                     /locus_tag="IXG44_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HAQ1657792.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            24005..24472
                     /locus_tag="IXG44_001060"
     CDS             24005..24472
                     /locus_tag="IXG44_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HAQ1657793.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
BASE COUNT         7901 a         4590 c         5376 g         6642 t
ORIGIN      
        1 gattatgaat tgaaagaacc aaagtataca gtagcagaag ctcgtgcgca tgatgcaaac
       61 tactctgcac cattacatgt aacattgcgt ttgacaaacc gcgagactgg cgaaatcaaa
      121 gcacaagaag tattcttcgg tgatttccca ttaatgacag aacaaggaac attcatcatc
      181 aacggggcag agcgagtaat cgtttctcag ttagtccgtt caccaggtgt ctatttccat
      241 ggaaaagtcg acaaaaacgg aaaagaagga ttcggttcta ctgttatccc taaccgtgga
      301 gcttggctag aaatggaaac tgatgcaaaa gacatctctt atgtacgtat cgaccgtaca
      361 cgtaagattc ctttaaccgt attagttcgt gcgttaggat ttggttccga tgatacgatc
      421 tttgaaatct tcggcgacag cgaaacatta cgcaatacag tcgaaaaaga cttgcataaa
      481 aacgcaagcg actcaagaac agaagaaggt ttgaaagacg tttacgaacg tttgcgtcca
      541 ggcgaaccaa aaacagcaga cagctcacgt aacttgctga atgctcgttt cttcgatcca
      601 aaacgttacg acttagcaaa cgttggtcgt tacaaagtaa acaaaaaatt agatttgaaa
      661 acacgtctat tgaatctgac attagctgaa acattagttg atccagaaac aggcgagatc
      721 atcgttgaaa aaggaacagt tctgactcac caagtcatgg aaacactagc tcctttcatt
      781 gataatggcc taaattcagt gacttactat ccatcagaag acggtgtggt cacagatcca
      841 atgactgtcc aagtcatcaa agtattttca ccaagagacc ctgaacgtga agtaaacgtg
      901 atcggaaacg gctatccaga agcagctgta aaaacggttc gtccagccga catcattgct
      961 tcaatgagct atttcttgaa cttgatggaa ggtatcggca atgtcgatga tatcgaccac
     1021 ttaggaaatc gtcgtatccg ttcagtcggt gaacttttac aaaaccaatt ccgtatcggg
     1081 ctagcacgta tggaacgtgt ggttcgtgaa agaatgtcga tccaagatac tgaaacattg
     1141 acacctcaac aattgatcaa tatccgtcca gttgtggcaa gtatcaaaga attctttggt
     1201 tcttctcagt tgtcacagtt catggatcaa acaaaccctc ttggcgaatt gacacacaaa
     1261 cgtcgtcttt cagccttagg acctggcgga ttgactcgtg accgcgccgg ttatgaagtt
     1321 cgtgacgttc actactccca ctatggccgt atgtgtccga tcgaaactcc tgaaggccca
     1381 aacatcgggt tgatcaatag tttgtccagt tatgcgaaag taaataaatt cggatttatc
     1441 gaaacacctt atcgtcgtgt agaccgtgaa acaggtcgtg ttactgacca aatcgactac
     1501 ttgacagctg atatcgaaga ccattacatc gtagcccaag caaacagccc attgaatgaa
     1561 gatggaacat ttgctcaaga cgttgttatg gctcgtgctc aaagtgaaaa cttagaagta
     1621 tctattgata aagtagacta catggacgta tcacctaaac aagtagttgc cgttgcgaca
     1681 gcatgtattc ctttcttgga aaacgatgac tccaaccgtg ccttgatggg tgccaacatg
     1741 cagcgtcaag cagtgccgtt gatcaatcct caagcaccat gggttggtac tggtatggaa
     1801 tacaaatcag cgcatgactc aggagctgct ttgttatgta aacatgacgg tgtcgttgaa
     1861 tacgttgatg cttctgaaat ccgcgtacgc cgcgacaacg gtgcattaga taaatacgat
     1921 gtaacaaaat tccgtcgttc aaactccgga acaagctaca accaacgtcc aatcgttcat
     1981 ttgggtgaga aagtcgaaaa aggggatact ctggcagacg gaccatctat ggaacaaggc
     2041 gaaatggctc taggccaaaa cgttttagtt ggtttcatga cttgggaagg gtacaactat
     2101 gaagatgcga tcatcatgag ccgtcgtttg gtaaaagatg acgtatacac ttctatccat
     2161 attgaagaat atgaatcaga agcccgcgat acaaaattag gacctgaaga aatcacacgc
     2221 gaaatcccta atgtcgggga agacgcatta aaagatttag acgaaatggg tatcatccgt
     2281 attggtgcag aagtccaaga tggcgatttg cttgtaggga aagtcacgcc aaaaggggta
     2341 acagaattat ctgcagaaga acgtctattg catgcgatct ttggcgaaaa agctcgtgaa
     2401 gtccgcgata catcattacg tgtaccacat ggcggcggcg gtatcgttca cgatgtgaaa
     2461 atctttactc gtgaagctgg agatgaattg tcaccaggcg taaacatgtt agttcgtgtt
     2521 tatatcgttc aaaaacgtaa aatccatgaa ggggataaaa tggccggtcg tcacggaaat
     2581 aaaggggttg tatcccgtat tatgccggaa gaagatatgc cattcctacc tgatggaaca
     2641 ccaatcgata tcatgctgaa tcctttaggg gtgccttcac gtatgaacat cgggcaagta
     2701 ttggaattac acttgggtat ggctgcacgt cagttaggta tccacgtagc aacgccagta
     2761 tttgatggtg caagtgatga agacgtatgg gaaacagttc gtgaagccgg tatggccagc
     2821 gatgcgaaaa ctgttctgta cgacggacgt acaggtgaac catttgatgg acgtgtgtct
     2881 gtcggtgtga tgtacatgat caaacttgct cacatggtcg atgacaaatt gcatgcacgt
     2941 tcaatcggac cttactcatt ggttacacaa caaccattgg gaggtaaagc tcaatttggt
     3001 ggacaacgtt ttggtgaaat ggaagtatgg gcactggaag cttacggtgc ggcttatact
     3061 ttacaagaaa tcttgacata caaatcagat gacgtagtcg gacgtgtgaa aacatacgaa
     3121 gcaatcgtca agggtgaacc aattccaaaa ccaggtgtac cggaatcatt ccgcgtattg
     3181 gtgaaagaat tgcaatcact aggtctggac atgcgtgtct tggatatcga agaaacagag
     3241 atcgaacttc gcgacatgga cgatgaagac gacgatttga tcactgttga tgctttgaca
     3301 aaatttgccg aacagcaaac tgccaaagaa ttagaaaaga aagctgctga acaagtggaa
     3361 gatgaaaaag acgatgtcat ccaaaacttt gaaaccgcag aagataattt agactaatcc
     3421 ggtctatgag ctgcaaggcg gtcgaagatc agccgtcttg cggtttgcca ttaaatgaaa
     3481 tggagggaac cccttttgat cgatgtaaat aaattcgaaa gtatgcaaat aggtttggct
     3541 tctcccgaaa aaatcagaag ctggtcttac ggtgaagtaa aaaaacctga gacgattaac
     3601 tatcgtacct taaaacctga acgcgaaggg ttgttctgtg agcggatttt tggtcctaca
     3661 aaagactggg aatgtgcctg cggaaaatac aaacgtatcc gttataaagg aatcgtttgt
     3721 gatcgatgcg gagtggaagt tactcgttcg aaagttcgcc gtgaacgcat gggacatatt
     3781 gaattagcag ctcctgtttc acatatctgg tactttaaag ggattccttc acgtatgggt
     3841 cttgtattag acatgagccc acgcgctttg gaagaaatca tttattttgc ttcatatgta
     3901 gtcatcgaac caggaaatac gaatttagag aaaaaacaat tgttgacaga acgcgaatat
     3961 cgcgaaaaac gtgaacaata tggacaagaa ttccaagctg ctatgggtgc agaagcgatt
     4021 aaacaattac ttgataatgt tgatctagat gcagaagcag ccgaattgaa agaagaattg
     4081 aaatctgcac aaggacaaaa acgtacacgt gcaatccgcc gtttggatat tttagaagca
     4141 ttccgcgctt caggaaatca accaagctgg atggttatgg acgttatccc tgttattcca
     4201 ccagatttac gcccaatggt tcaattagaa ggtggacgtt ttgcaacaag tgacttgaat
     4261 gatctttacc gtcgtgtaat caaccgtaat aaccgtttga aacgtttgtt agacttgaat
     4321 gcacctggaa tcatcgttca aaatgaaaaa cgtatgctgc aagaagcagt agatgcattg
     4381 atcgataacg gtcgtcgcgg acgtccagtt actggaccag gtaaccgccc attgaaatct
     4441 ctttctcata tgttgaaagg gaaacaaggt cgtttccgtc aaaacttgct aggtaaacgt
     4501 gtcgactact caggtcgttc cgttatcgtt gttggacctt tcttgaagat gtaccaatgt
     4561 ggacttccaa aagaaatggc cattgaatta ttcaaaccat tcgtgatgcg tgagcttgtt
     4621 caacgcgaac tagcttcaaa catcaaaaat gcgaaacgta aaatcgaacg tcaagaagac
     4681 gaagtatggg atgttttaga agacgtaatc aaggaacacc cagtattgct gaaccgggca
     4741 cctacgcttc accgacttgg tatccaagca ttcgaaccag ttttagtaca aggacgcgca
     4801 atccgtttgc atccacttgt atgtgaagct tataatgccg atttcgatgg agaccaaatg
     4861 gctgtccacg ttccattgaa tgaagaagca caagcagaag cacgtatgtt gatgttagct
     4921 gctcaaaaca tcttgaaccc taaagatggt aaatcagttg ttactccttc tcaagatatg
     4981 gttctaggta actactactt gacaatggaa gaagaaggac gcgaaggcga aggaatggtc
     5041 ttcagagatc aaaacgaagc cgttatcgca tggcgcaacg gatatgttca tttacattca
     5101 cgtatcggtg tgaaccctaa ctcattaggt gaaaaaccat ttactgaatg gcaaaaagaa
     5161 cgtacgatga tcacaacagt tggtaagatc atcttcaacg agatcatgcc tcctgaattc
     5221 ccttatctga atgaaccaac agacttcaac ttgaccgtcc aaacaccgga caaatacttt
     5281 gttgaagctg gtactgatat tcctgcacat atcaaagagc aagaattagt attaccgttc
     5341 aagaagaaaa acttaggaaa catcatcgca gaagtcttca aacgattcaa agtaacagaa
     5401 acttctaaga tgcttgaccg tatgaaagac ttagggtaca aacattcgac acatgccggt
     5461 atcacagtag ggatcgctga catcagtgta ctgaatgaaa aacaagaaat cattgaaaat
     5521 gcccataaac aagttgaaac tattacaaaa caattccgtc gtggtctgat cacagatgac
     5581 gaacgttatg aacgtgtcat tgctgtttgg aatgctgcaa aagacagcat ccaacaaaaa
     5641 ctgatggaag gtttggaagc gaaaaatcca atcttcatga tgtctgattc tggagctcgt
     5701 ggtaacattt ccaactttac acagcttgcc ggaatgcgtg gtttgatggc cgctcctaat
     5761 ggtcgaatca tggaattgcc aatcatttcg aacttccgtg aaggactatc cgtcttggaa
     5821 atgtttatct caactcacgg ggctcgtaaa gggatgaccg atacagcctt gaagactgcc
     5881 gattcaggtt acttgactcg tcgtctggtt gacgtggccc aagatgtaat catccgtgaa
     5941 gaagattgcg gtactgaccg aggtcttgaa atccaagcca tccgcgaggg aaatgaaatc
     6001 atcgaatcat tagaagatcg tttagtggga cgttacaccc gtaaagaagt acgtcatcct
     6061 gaaacaaatg aattgatcat tgcaggaaac caattgattt ccgaagacat cgcaaaacaa
     6121 atcgttgatg caggtgtcga aacagtgaca atccgttctg tcttcacatg taatacaaaa
     6181 catggtgtct gcaaacattg ttacggacgc aaccttgcaa ctggctctga cgttgaagtc
     6241 ggagaagcag ttggtacgat cgccgctcaa tcaatcggtg aacctggtac tcagttgacg
     6301 atgcgtacat tccatacagg tggggttgcc ggagacgata tcactcaagg tcttcctcgt
     6361 gtccaagaaa tctttgaagc acgtaatccg aaagggcaag cagtgatcac agaagtaaca
     6421 ggggatgtca tcgatatctc agaagatcct gcaacacgta ccaaagaagt aacaatcaaa
     6481 ggaaaaacag atacacgtac gtatactgtt ccttatacag cacgtatgaa agtagcagaa
     6541 ggcgatatga tccaccgtgg tgcaccattg acagaaggat caatcgatcc aaaacaattg
     6601 ttgcaagttc gcgatgtatt atctgttgaa aactacttgc ttcgtgaagt acaacgtgtt
     6661 taccgtatgc aaggggtaga aatcggcgac aagcatatcg aagtaatggt tcgtcaaatg
     6721 ctgcgtaaag ttcgtgtaat ggatccaggt gatacagata tcttaccagg tacattatta
     6781 gacatcgcag actttaaaga tcaaaactac aatacgctag tctcaggagg cgttcctgca
     6841 acttctcgac ctgtcttact aggtatcaca aaagcatcat tagaaacaaa tagtttctta
     6901 tctgctgcat cattccaaga aacaactcgt gtcttgacgg atgctgcgat ccgcggtaag
     6961 aaagatcctc ttcttggttt gaaagaaaat gttattatag gtaagatcat cccagctggt
     7021 actggtatgc aacgttatcg tgacatggaa ccaaaagaag tcggtgtagc aagcgaaaat
     7081 gtctacagta tttcagatat tgaagcgcaa atggctgcag aagacgcaat gaacaatcaa
     7141 acaaaataga tattttaaaa aagaggttgt gacaaattat tgtcacaacc tcttttttgg
     7201 tttgtctaaa ataatttgtc taactagaag aaaagtggga aaggatctta acaacatcgg
     7261 ttcgtatcca attaaggtat cggaacgatc atcttccaag agaagtcaat caacaaaagt
     7321 aaagatagat cattttttga atgcaaaata aattgatggg aagatagagc ggttattttt
     7381 aatattttgc tagctattat tgaataattc ctcgaataaa acaaaaccag tagacatgtc
     7441 tgttattcta tttatgtttt agagataagt atccccaaat aacatggatt gaaataaggg
     7501 taaatgtgtt taaaaaaacg aactataaaa acttatacac aacgtggtta aaaatagttt
     7561 tttcttccct gtggaaaaaa tagtttaaca caaacattat agttatccac agctatctaa
     7621 aacagatgga aaataagaaa aattcgctaa aataccggga gggggtaacg agggatgagt
     7681 gattgaaaaa agaacagagt aactgactta tcctcagtgt ttctgttctt ttataattct
     7741 gttctacatt tcttgttgtt ggatcgaatc tgtgagagta atctcggttg aaagaatgat
     7801 tcttttttct tcagggtatt ggtggtggat tagttggaat aaaagttctg tggctttgat
     7861 tcctaactga tttgtcggaa tgtctatccg agtcaactgt ggcgaaaata gggaggacaa
     7921 taagggatca ctgaatcctg cgatcgaaag atcatcaggt atctgctttc cagattgttc
     7981 tgccactttg taaatactca atgattttaa tgcatcggta gaaaaaatcg cagttggtct
     8041 ttctcgtagc ttcattaaat cattcattgc tgccatgcta tcttctaagc gaaagccgcc
     8101 gtctataatc cattcattac ggatatctag gtggtgatca aataagcatt gacggtaacc
     8161 attgagacga tcattggata cataaacttc tgtcggagca tggagacagc cgatatgacg
     8221 gtgtccattc ttgatcagtg tttccaccaa atcgtaacta tcctggaaat tatccgtgtc
     8281 cactgagtag atgttcgtgt agtcgtttcc gacttttccg attaccacaa taggaatatt
     8341 gtattgatcc aattcaaaaa gaagtgtttc atccatcgta gagcttaaaa taataattcc
     8401 tcggatcatt ttttcttgga tctttttcgc gcatttttct agctcttctt ctggtgtttt
     8461 tgccgtttgt agaatcaagt cgaagttatt ttgttcagaa acggcagaaa tggattgaat
     8521 aacatctgaa aaaaaaggat tgccataggt cgtatgagaa gtacgatgag ataatgctag
     8581 aatcgcgtca aaaccatttc ctgacagtcc tcttgcaatt ttgctcggtt cataattcaa
     8641 ttctttgatt gcttgaagta cacttgcttt tgctttatca gagatatttg gctggttatt
     8701 taatactctg gagacagttg attttgagac attagctttt ttcgcaatgt cataaatagt
     8761 aggtttcaat tttatatgca cctccttttt gggagcgttt tagaataatt ccatagtaca
     8821 atagacattc ttttttgtct atgattttga aaatttagga atagcttgca tgatcatgag
     8881 agaccatatt tttttaagaa gctattaatt attgatacat aagctttctt atttgtattt
     8941 ttgtgtatat caaaattatt taaaaaaatg ctattgacag taagcgtttt ccataatatg
     9001 atcaaatcgt tgggaacgct cccaaaaaag aaaggggaat ccgatgtata caaaactttt
     9061 acaagcgatt tatcgcgact cagatttgtc tgagaaaacc gcctctatga ttgaacaaaa
     9121 agtcagtgtt tatcagcaga agaaaggcca ggcaggcaaa ctgcattggg acgaagaaga
     9181 tattttcttg attacttatg gggatcaatt ttttgaagag cagcaaccaa ctttgcgtac
     9241 atttaagaaa ttttatcaaa agtatttggc agatacattt agagttgtac atttcttgcc
     9301 atttttccca tatacatctg atgatggttt ctccgttgta aattatcagg caatcaacga
     9361 ccgtttgggc acatgggaag atatagaaga aatgagaagc gaaacacggt taatgttcga
     9421 ctttgtctgc aatcatatgt ctgccgaaag tacttggttt caggaatatt taaacttgaa
     9481 tgatacgtac aaagattttt ttattgattt gccactggat acagatacaa gcagcgtgac
     9541 tagaccaagg accagcccgt tacttacaac gttcattgac aaaaacggga aagaaaaaca
     9601 cgtatggacg acttttagcg aagatcaagt agacttgaat tttaaaaatc cagaagtttt
     9661 attgaaaatg atcgatattc ttttgtttta cttagaaaaa ggcgcttcat ttattcgctt
     9721 ggacgctgtg ggatttttat ggaaagaact aggaacttct tgtatacacc tgccagaaac
     9781 gcataaaatc atccaattat tccgtgcaat cgctgatgaa gcagctccag gaacggtatt
     9841 gatcactgag acaaatgtgc cacacaaaga taatatcagt tattttggga atggcgagaa
     9901 tgaagcacag atggtctatc agttttcgct accacctttg acccttcaag cgattcgttc
     9961 gggtaataca agtaagttga aagaatggtt aaaaaccatc gatttcgcat cgaaaaaaac
    10021 aacttttttt aattttttag cctcccatga cggtatcgga ttgaatccgg tacgaggtat
    10081 tctgccggaa aaaacgatcc ttgaattagt ggaacagctg aagaaagagg gtgcccgagt
    10141 aaactacaag aaaaacccag atggaacgac cagtccatat gagataaacg ctacgtatct
    10201 agatgcgtta agttctccaa cagatacgac ggaatggaga agcaaacgct ttttagctgc
    10261 tcattcattt ttggtaacgt taccaggcgt gccggcaatt tatattcaaa gtgtgctggg
    10321 aagtcgaaat gattacaaag gcgtagaaaa aacaggagag aatcgaaaaa tcaatcggca
    10381 aaaatttttg ttagcagata ttgagaaaga gttgcagaca gatccattaa gaacagcagt
    10441 atatggtgaa ttgacccaga tgctatctat tcgaaaaaaa gaaaaagcct tccatccgaa
    10501 tagtttgatt tacttactgg actcagcaga ttcttttatc agttttattc gcgaagcgaa
    10561 ggaagagaaa gtcttagtta tccataattt ttccaatcaa tcagcaggtt atcttttacc
    10621 aaaaggagat tacctagacc tgttttctgg acaaattatc cattgcagtc aagaacgaaa
    10681 gttgatattc cagccgtatg aatatatgtg gctgaaaata ataaaataag aggtggaaaa
    10741 aaatgaaaaa aattattgtt actacctgtc tgctagctac tggatttctt cttactgctt
    10801 gtggtgaaag tcaaagctct aacgaatcac aacagacaac gattcaattc atgcactctt
    10861 ctgtcgaaca ggaacgatta agtgtgatca atgaactggt tgctgatttt gaaaaagaaa
    10921 atccagacat caaaatcaag caagtgccgg tagaagaatc agcgttcaat acaaaaatcg
    10981 ttacgttagc tcgttccggt aaattaccag aagtaatgga agtcagtcag gattttgcta
    11041 aagtgatgga taaagatgaa ctgattgatc agacggcagt ccaaaacgtt atcgaggaag
    11101 ctggagaaga taattattat gacggagcca aaaaccttgt tcgttcagaa gatggaaaaa
    11161 gctatattgc tgcaccaatt agcggctggg ttcaaggtat ttggtatgac aaagaaaaat
    11221 tatcagaagc tggtttttca gaaccagaaa attgggatga tattttaaaa attgctcaac
    11281 atttcacaga taaagagaat aaacaatatg gaattgctat gcctactgct gaaggaacaa
    11341 tgagtgagca agcgttctct caatttgctt tgtcaaatgg agcaaatgtt ttggatgatg
    11401 aaggcgaggt aacgatcgat acggaaaaga tgagagaagc attgtcgttt tatcaaaact
    11461 tatcccaata cacgatgcca ggctctaatg atgtgacaga aatcaaagat gctttcatga
    11521 atggaacagc accaatggca atctattcaa cttacatttt gccttctgtt tatgaagcgg
    11581 ggaactcaga aaatatcggt tttgctatcc caacacaaaa agatcaagct gtctatggca
    11641 cagtatctgg attgacgatt tcttctggtc tagacgagaa acaaaaagag gctgctgaag
    11701 cattcacagc tttcttatct caaccgaaaa acatggaaaa gtgggtcttg atgtctcctg
    11761 gtggtgctca accagtgaat aaacaagtag tcgaactgga tggttacaaa gaaaatgaag
    11821 tcatcaagtc ttttggcgag ctgccatctg agattgctag tgcttttgat gaagtacaag
    11881 tctttggact agtcggggaa aagaatttca cgaaaatggg agatatcaca agctcaggtg
    11941 tgatcggaaa agcagtgaac caagtaactg tcggaaacga agatgtcgat caagctttag
    12001 ctgacgcaca aaaaaatacg aaataacaag aagttctgcc aatggtgttc gaaaaaagta
    12061 gatagctatt ggcagattat cagggggagg tttcaatgaa tcgtccaagg aaacattctg
    12121 acttaaaatt agccatggga cttttagcac caagcatgct tttattagct gttttagtgc
    12181 tgattccaat gctttccaat attcagatca gttttttcga tatgccgctg aatcctaact
    12241 cagacgccgt ttttatcgga ttaagcaatt atcttgaaat tttaagtgat agtgaatttt
    12301 atcgttcttt aggtttaaca gttgcttaca cattcttagt tgtagcaggc agtacagctt
    12361 taggattagc cgtagctatt ttgcttaacc gcaagttcag atttaggaaa acggtgcgtt
    12421 cactgatttt attaagttat gttacgccat ctatttcttt gatttttgct tggaaatata
    12481 tgttcaataa tagttatgga atcgtcaatg atctgttagt agataggttg cagcttttca
    12541 acgaagcacc gctgtggttc gaccagccag ttgctagctt ctttatggtc gttttgtttg
    12601 ctatctggag atactatccg tatgcgttta tttcattttt ggctattttg caaacagtcg
    12661 acaaaacgct ttatgaagca gctgaaatgg acggtgcaaa tgcttggcag aagttcaaga
    12721 tcgtgacact gccggcaatc aaaccagtac tagcaactgt gattgtttta cgatctatct
    12781 ggatgttcta tatgtttaca gatgtctatc tactgacgaa taaagtcaat atcttaggcg
    12841 tttatttgta tcagacagcc tttgcgttca atgatcttgg aaaagcagca gctatatcga
    12901 ttctattgtt catcattatt gcagcggtcg tcttgctctc acgaaagaag gtggacttga
    12961 aatgaaaaat aaaaagagca ggaaaatctt attttacgcg acgattttgt tgctattagc
    13021 tgtcaatttg tttccatttt tgatcatgct gatgacatca ttcaaggatt cacaagaagc
    13081 aattgcaacg acacaaacta tttttccgaa agaatggact tttgaacatt atcaggatat
    13141 tttcaatgct gagatttttc cattcatcat gtatttcaaa aatagtttag tgatctcctt
    13201 gatagcctcc gtaactgcag tagtgattgg gatcagcggg gcatacgctt tgtcaaaact
    13261 acgcttcaaa gctcgctcga aaatcaatgc tagcttttac accgtttata tgttttccgg
    13321 tattttgatc gttgttccat tatttaaaat catttccggt ctaggtttgt atgataccaa
    13381 tacagcactg attatcacga tggtcgttca aacacttcct actgcgatat tcatgctaaa
    13441 aagttatttt gacacgattc cgactgatat tgaagaagca gcaatgatcg atggtcttag
    13501 ccggatccag atcattattc ggatcattat accattggct atttctggaa tcatttctgt
    13561 cttcgtttat tgctttatgg tttcgtggaa tgattattta tttgcttcga ttttcctgtc
    13621 gacctccgaa aaatttactt taccaatcgg tttgaacaca ttattcagta caccagacta
    13681 catttgggga cgaatgatgg ccgcttcatt ggtgaccgct ttaccagtca tcattatgta
    13741 tgcgttatct gaacgattta ttaaaggtga tttagtcgaa ggcggcgtaa aaggataaaa
    13801 agaaaagaaa gaggaaagaa aagatgaaaa aattagttgc attagaacca agaatagccg
    13861 gattcaaaga ttacgaagac cgtgagatcg aaaagaacga agtacgtatc cgagtaattt
    13921 tcgctactcc aaaaccagga acagaaattg tcgactttag aggattaagt ccatttatcg
    13981 atgaagaatt ttcagaagaa tggcgcctat tcttgccaag agaagaagga agccaacgcg
    14041 gtgtggtatt tggcgaattt caattaggaa atatgattgt cggtacgatc gaagaaacgg
    14101 gcagtttggt gacagagtat caaaaaggag agttagtctg cacttatgga cccattcgtg
    14161 aaacgctgat tacaaacggt gttgataatt ataaactacg taaacttgat cggttggatc
    14221 aatggaaaaa tgcagtatgt tacgatcctg ctcaattcgc tttaggtggt atccgggaag
    14281 cacatgttcg agcaggtgat gctgtagcaa tcttcggttt aggcgctatc gggcaaattg
    14341 cgattcaatt agctaaaaaa gcaggtgcta ctaccatcgt tgggatcgat cccatagcgc
    14401 agcgtagaga attagcagaa aaatacggtg ccgattacac gctagatcct atgaaagcag
    14461 atgtcggttt tcgtctaaag gaattgacag ataaacgtgg agtagattca gttattgaaa
    14521 cgagtggcag tgctgccgca ttacaagctt ctttaagagg gatcggctat ggcgggatca
    14581 tttcttatgt tgcctttgct aaaccttttc cagctgggtt taatcttggt cgcgaagcac
    14641 atttcaataa tgcaaaaatc gtctttgctc gagcatctag tgaaccaaac ccagattacc
    14701 caagatggaa ccgtaaacga atcgaggaca catgttggga attattgatg aacggttact
    14761 tggattgtga agaaatcatt gatccagttg tgccatttgc agaaagcgca gaagcttaca
    14821 tgcgttatgt agaccagcac ccagaagaaa gtatcaaaat gggcgttcaa gtccgaggag
    14881 gagaacaatg aaattcggta cgcaagatca agcatttttc ccaagagaca ttttagaaaa
    14941 atttagattt atcaaagact gtggatttga agaatacgaa atcgacggta agttgttaat
    15001 agaaaatatc gacacagtca aacaggcaat caaagaaaca gggatcccag tagtgacagc
    15061 ttgtaacggc tacgatgggt ggatcggtga ctttatcgaa gaacggcgtc ttaatggtgt
    15121 acgtcaaatt gctgagattt tgcgtgcact aagcgaggtt ggaggcaaag gaatcgttat
    15181 cccagcagct tggggaatgt tcacttatcg attaccgccg atggtttcac cccgaagcaa
    15241 agaaggagac tataaagcag tcagcgaatc acttcgttat ttagataaag tagccgaaga
    15301 aacaggtacg gttatttatt tggagccttt gaaccgatac caagaccata tgatcaattt
    15361 gttagcggat gcgcgtcatt acattgactc taatcaattg aaacatgtgc agatcatcgc
    15421 tgatttttat catatgaata ttgaagaaga tgatatttca aaagcattga ctacgcaaaa
    15481 agagtatgtg ggtcatatcc atttagcaga taatcatcgc taccaaccag gatctggcgc
    15541 aattgatttt actcgccatt ttcaaacgct acttcaaaat gagtatgaag gttatatggt
    15601 atatgaatgt cgtttgcgag gagaaaatga agtgaaaatt tatcaggaat cattgcaata
    15661 cttgaaaaac tgtctggaaa cagcgcgtca tttaaatgat ttagagaaag aatagagaaa
    15721 gaataacaat aagtaaataa gcaaaggagg gagaaataaa tgaagaaagt agcaatcata
    15781 ggcgctggac aagtagcaga aaaagtacat gctgcttatt atcaaaaaag atcagatgtc
    15841 ttagaattgg ccgctgtggt tgatccagtc agccagcgag cagaagactt ttgccgacgt
    15901 acaggatttt ctcatgcgta tacatcagtt acacaaatgc tgaatgagat ttctcccgat
    15961 atcgtgagtg tctgtacacc caatcgcttt cacttcgaac atgtattggc tgccctcgaa
    16021 gctaatgctt cagtgatgtg cgagaaacca ccagctatga cagctgatca agcaagaaag
    16081 atggcagaga cagccgataa aaacgggcag attttggctt atggattcca gcatcgtttt
    16141 tctgacgaag caacaatgat caagaatcat cttgatgttc tcggggatat ttattacgta
    16201 gaagcgaatg cgcttcgtcg gtctggtgtt ccaggctggg gaacgtttat cgacaaagaa
    16261 cttcaaggag gcgggccttt gatcgattat ggcattcaca tgcttgatac agcgatgtat
    16321 attactggat ttcctaaagt aaaaagcgtt cgggcgtaca gcttccaaaa aattggtacg
    16381 aaaaaaaatt cagggaaatt cgggaaatgg gatccaagca aattcacagt agaagatgca
    16441 atttttggca cactggaatt agaaaacggc aagatcatcc aactgaatac ttcgtttgct
    16501 ttgaatatca gagaagacaa actgaatgtg gctttttgtg gcgatcaggc aggtgctaat
    16561 ctgtatccag gcgaactatt cgaagatgta gatggaaagc tggtttcctt acaaaagacc
    16621 acgccagtta atgacccaca tgaactaagt atggctcatt ttgtgaatcg ggcgctcggt
    16681 gaccagactc agatgattgc agatggctgg caaggatatg tcatccagaa tctggtggaa
    16741 aatttatatc gttcggcaga aacaggagaa gttattacct tatgaaagta gaagaaaaac
    16801 agtttgattt agagtatatc aataagtatg cgagtcagat gaccattgga aatggatttt
    16861 ttggtatcag aggaagccag gaggaagact atcggaatca agtaagggga atgtttgcag
    16921 ctggtgtata taaccgacct ttaggagcag aatcagccga gttagtgaat ctcccagatg
    16981 tcatcagatg ggaaataaaa atcgacggtg aacatttttc tcttgagacg atgaaagttc
    17041 ttgcatacga gcgggcacta gatttagaaa tgggagagct taaacgctat gcgctcattg
    17101 aaactgctca aggaaaaaaa gtggaagtca ttacttgtcg tgttgctgat caaaataatc
    17161 tccatcaggc agccctgaaa gtggtcatca agccgttgac cgaagactgc caagtagatg
    17221 tgaaaacggg tatcgatgga cagcaaacca attttggtac acagcagttg gttgaaaaag
    17281 aactgcgagt atttggtgac gatttgatca gtgcatccta ccagacaaca gaaagtaaaa
    17341 ttgatattgg gattgcagct aaattcaata aagcaggtgt attccaagca aaaaatcgcc
    17401 ggatcaccgc ttccttttca gaaaagatca gaaaaaatac gtcctttacg ctggagaaga
    17461 tcatcgtgct ctctacctct cttcagcaaa cagacccgtt taagaaaagt attgcccaag
    17521 cccagaaaag cgttagttat acagaaattt tagagcagtc gatgtcttat tggacagatt
    17581 tttggcatca gcatcggata acgatccaag gcgattcttc tttcgatcag ttagcgatgg
    17641 acttcgctgc ttaccattta cagattatga ctccccgcaa tgatccaagt gttagtgtag
    17701 gggcgaaggg actaactggc gaaggttata aagggcatgt tttctgggat acggaaattt
    17761 ttatcctgcc tttcttttta tatcagtttc ctgaagtagc aaagaatttg ctgctgtacc
    17821 gttatgatcg attagaaggc gcacggcaaa aagcaagaga ttatcactat caaggtgcac
    17881 ttttcccatg ggaaagcgca cacactggtc aggaagagac acccaaattt gctgcattga
    17941 atatcaaaac cggaacaaga caagaagttg cttcagctgc cgcagaacat catatcagtg
    18001 cagaccttgc ttatgccgtt caagagtatg tccaagcaac agaggatcaa tcgtttttag
    18061 aaacaaaggg aaaaacgctc attcatgaaa cagcagcttt ttggctgagc agagctgtaa
    18121 aagtcgatga tcatttagag atacacagag tcattggtcc agatgaatac acagaatata
    18181 tcgacaataa tgcatatacg aactatctgg cgcattataa tgtggcctta gctgttaaat
    18241 atcaagctgg cgatccgctt tttttgaaag aatgtcaaaa ttttttagat aagttatatt
    18301 tgccagtgcc taacaaagaa cagctgattc cgcaagatga cacgtatctc agtaagccag
    18361 ttattgattt gaccacttac aaagaacgtc ctggcagcca gtctatttta cttgattatg
    18421 cccgtagcga agtaaatgaa ctccaagtca gcaagcaagc agatcttgtc atgctgttat
    18481 acttattacc tgagtatttt tctcaaccaa tcgtttctaa gaatttgcat tactatgaaa
    18541 acaaaacaat ccacgactca tcgttgagta aagcgattca cgccatagag gcttgccgta
    18601 tccaagaaat ggaatgggcg tatcatttat tccaagaagc gtgcctgatt gatttaggac
    18661 cagatccgca ttcatcagat gacggaattc atgctgcttc attaggagca ctatggctag
    18721 cagttatatt tggtttttca ggtattacag taacgaatgg aactctttct atttctccaa
    18781 gacttccttc agcatgggaa aaaatttctt ttccttttat ttggaaaaat cacaggttgc
    18841 tatttgtttt gacaaaaaaa caaatagtaa taaaaaaaga gacgaaagaa acattaccta
    18901 tttttgtcaa tggagaccaa taccagctgg aaaaagaact gatcttggaa cggaggggaa
    18961 aataatgcgt attctgattg cgccagattc tttcaaagaa agtctgacag ccttagaggc
    19021 agcacaagcc attcaatcag gttttcaaga agtttttgtt caggctgaat atgatctgct
    19081 acctataggc gatggaggag aaggaacggt ttcatcatta gcagacagtt taaggttgaa
    19141 gcggcaaaca gcagtcgtta ctaatgcatt tggcaaaaac aaagaaatcc agtacgcttc
    19201 agacggtcaa actgcgattt ttgaaatggc agaaatcgtc ggattgaaag atgtcccact
    19261 aaaccaacaa cagccactta ggttatccac gcgaggagta ggagaactga tacgacaatt
    19321 gttgcaacaa ggtgttcgac acatcatcat cggcgttggc ggaagtagta ccaacgatgg
    19381 cggcatcggc atggcagaag gtctaggtta tgctttttac gatcaagata atcaaaaagt
    19441 agaagcactg ggagaaaatc taggtaaaat tgctacagtt gataaaacga acgtgcctat
    19501 tgagctgatt aaagccaaaa tcactgtcat tactgatgtt actaatcttt tatgcggaaa
    19561 agaaggcgct tcttttgttt tcggaccaca aaaaggcttg aaagaaaatg aattagcagt
    19621 ggctgaccaa gccatgcatg atttttatat gagatttgcc cctcaagtaa tcgaggcatc
    19681 gggcagtagt gctggtggtg gaatgggtgc aggactgtta gcatttgctt cagggaaaat
    19741 agtctctggc atcgactacg tattggattt actggagttc gataaacggg tacagcaagc
    19801 agacctcgta atcgtaggcg aaggacgtct agatggacaa agtctaaaag gaaaagcgcc
    19861 agttgggatt gctagaagag taccagaggg cgttcctgtg atagctattt gcggaagtgt
    19921 tggtaaagga agcgaaaaag cagcagatgt tggcatttcg gccgtttttc cgatccttcc
    19981 tgcagctgtg cctttacaag aagctttaca gcaagccact gataatcttc atcgcacagc
    20041 taaaaacatc gcatctttac ttcaagtcgt tcattttcaa ggaggtaatc gatgaaaatc
    20101 aaagcagcac tattcgattt agatggtgtt cttgtagaca cagctcgtta tcattacgaa
    20161 gcgtggctgg ttttagctaa ccagctgtcc attccattca ctgagaaaga aaacgagcaa
    20221 ctgaaaggaa tatccagaac cgagtcacta gagcgattgc tgtcatttgg aaaaatggaa
    20281 cagaaattct cagaaaaaga gaaatcagcc tttgctgagc aaaaaaacaa tctatatctt
    20341 caagcgattc aaaaaatgga tgaaacatcc gttttgcctg gtgcaatagc tgttcttgag
    20401 tacttgaaaa aaacaaacat caagatcgga ttgggatcag caagcaaaaa tgcacgcctc
    20461 attttagaaa aaacgaattt gacaagttat tttgatgtcc ttattgacgg cacgcaagta
    20521 tcaaaagcaa aacctgatcc agaagttttt ctcaaaggag cacagcaatt gaatgtaccg
    20581 ccaaatgcat gcttggtcat tgaagattca gaagctggtt gtcaagcagc tttggctggt
    20641 aacatgcatg tattgggaat aggtgaaaac ataaatcttc cttctgctga atatgtgatt
    20701 cctgatttaa ccgtttttga tcaagtacgt tcgttttggc atttatcaga agccgtatca
    20761 cgtatataat agtttccgtt ttttctgtca caagcaaaac gttatcctcc ttttggaaat
    20821 caaggtatga tgagaaaaaa gggggatttt atatgacaaa aaaagcagtt ttcgcaggcg
    20881 gatgcttttg gtgtatggtc aaaccatttg aagagcagcc agggatcatt tctgtgacat
    20941 ccggttatac tggcgggcat gtaccaaatc caacttatga acaagtaaca acaggcacaa
    21001 cagggcatac agaagcagta gagatcgaat atgatccaga agttatttct tatgatcagt
    21061 tggtggaaat ctattggcag caaaccgatc cgactgacgc attaggacag ttcgctgatc
    21121 gcggtgattc ttatcgtcca gtgatctact ataatgatga agaagaacga aatattgcag
    21181 agcgttcaaa acaagcattg caagacagcg ggcgattcga taagccaatc gtgacaaaaa
    21241 tcgaaccaag aagcgaattt tatcctgcag aagattacca tcaggattat tacaagaaaa
    21301 acaagctcca ttacaatctt tatcgagaag gttctggacg tgcaggtttc cttcgtcgac
    21361 attggggcgt caagaaataa gaagccgaaa aagctactta tggaacaccg tatatcagag
    21421 gttgtaaaac cagcattttg agctaatact gagggtcagc tgattgaaca ccatttagtg
    21481 gagaggttgt gaacctgtcg cctagctcca agtcacaagg atttggtaag agaacactat
    21541 atatcagagg ttgtgacaag tcgtttgtca caaccttttt tcaataccta agatgaagga
    21601 caatcagcaa cgcactgctc aaaaaaggaa tgaaaggcag cttttccaac ttttttttat
    21661 gacagataaa gtaaacagaa aaaacggata ttccaaacag actagcaaga aacaagatct
    21721 gaagcaactc ttctagagac aagccaccac tccaagaaag catcaacaga gtatcgccag
    21781 ctccgagata ggattgacca tagcgaaggc acacaccgcc tacgataaac gttaacagca
    21841 cactttgcca ttggaagacg ccggcctgca accaaaaaat ccataaaatt gcccaagaac
    21901 agaatagcgt gtgagaatgg accatgaagt aaaataaatc catcaacgaa aatagcaaag
    21961 ccgaaagcat ccatatgaac gtcgaaaaat ttcttgcgga agaaaaagta aagaaccagg
    22021 caaaaagacc accacataag gcttctgcca acaaatagta gaagggcaac cgacacctgc
    22081 aatagcggca gcgaaatcgt tggacgatga tcgagagaat cggaatcaat tcataccaag
    22141 aaagcgaacg acagcatttg acacaatgag atctaggata aataatagag tgacctaacg
    22201 gaatccgctg ggcggttaaa cataaaaatg atccgataca gcaaccaatg ataaaataaa
    22261 gtaacacgcc atctccccct tttcaaaacc aagatacgca agcttaaact aaatttttgt
    22321 acatacataa aatttgaggt gaaaaaatga acgtttcaat cggattagat atcggaacca
    22381 cacaaaccaa agcagttgct ttcacagatg aagcccaaga agcagcatcc gcttattttc
    22441 gctacccttt gatccaagaa acacaaggga tggcagaaca agatcaggaa ttgatctttc
    22501 aaggtgtctg caccgttata aaagaagtaa ttaaacagtt gccttcagac acaatcattc
    22561 aatttgtctc cttctcctct gcgatgcatg gcctgatatt gttggatgaa gagaaaacac
    22621 cgttatctcg aatgatcact tgggcagata accgagcagc ggacgaagca gaaaaactga
    22681 aatataccgt gcttggcaaa gaattttatc ggcagactgg gacgccgatt catccaatga
    22741 caccattgaa caaaatcaag tggctgcaaa acagccaacc tgaattattc aaaaaaactg
    22801 cttattttgt cggtatcaaa gactatatct tttatagatt gtttggcgaa ctgatcagcg
    22861 actacggaac agcttcagga actggatatt ttgatatcca tgaattccaa tgggcagaag
    22921 aagtgcttga ctacttgaac attcgaatag agcaattgcc gcaagtcttt ccttccacct
    22981 atcaagtaac aggtctacca tcaaaaacag cagcagaact aggtttgcca aatgatatat
    23041 catttgtttt gggaggggca gacgggccgc tttctaatct agggttaggt gcttttggtg
    23101 atacgatcgc cgctttgacc gtaggaacga gcggagcact tcgttttatt actactcagc
    23161 ctcagttcca tccagaaatg gagacgtttt gttatgttct ggatcagaaa cattgggtag
    23221 tgggaggagc gactagcaat ggtgctggca tctttgattg ggcagggcat acatttttac
    23281 aggaagtcgt tcaatctgct aaagaaaaag gagaaaatcc atatgatgca ctgctttcgg
    23341 ctatcgcttt tgtgccagcg ggtgccaatg gtctgatttt tcatccctat ttacttggtg
    23401 agcgagcacc gctttgggac gcagatgcgt ttggcagttt tatcggttta cagcggcaac
    23461 atacagaaaa agaaatgatg aaggcgattc tagaagggat ctgtctcaac ctttatcgga
    23521 ttttaaaagg agtttgccag gaaaatcagc tcacagagat ccgtgctaca ggcgggtttg
    23581 ctcaatctcc gatttttcgt cagatgatgg ctgatgtact gggttacccg cttgttttcc
    23641 ctactgtcac agaagcatcc gcattaggag ctgtgatttt aggctggaga agcatcggaa
    23701 agatccaaag tatcagtgaa gcacaggaat tgatagaatt gaaagagcaa gttggagtac
    23761 aagaaactga tcataaaaaa tatcagaaaa tctatccatt atttgtttcg acacaaaagc
    23821 atttagcaca gacttatcag caatttgctc aactgcgaga agaattagct gattctgaaa
    23881 aatgattggc tttacggata aaaaagcgaa ctgaaaatcg ttctcaggta aatgattttg
    23941 ttgtactttt cattgaaagt ctactattat tttaagtgta agaaaatgtt aggaggcaag
    24001 aaagatgaaa ttccaacaaa ccaaagaagt attaaatcaa ttagttgcag atctaagcca
    24061 aatgtctgtt gttgttcacc aaacacattg gtacatgaga ggaccaggtt tccttacatt
    24121 gcacccaatg atggatgagt tcatggatga tttgaacgct cagttagatg aagtatccga
    24181 acgcttgatc acattagacg gttcacctta ttctacattg cgtgaatttg cagataacac
    24241 aaaaatccct gatgaaattg gccgctggga tcgtactatt ccagaacgtt tggaaacact
    24301 tgttgcaggc tatcgttatc tagcagatct ttaccaaaaa ggaatcgaag tttctggaga
    24361 agaaggcgac gatccaacac aagatatctt tatcgaattc aaaacagcca cagaaaaacg
    24421 tatctggatg atccaagctc atttgggcaa agcgccagag atcgacgcgt aaaaactgtt
    24481 gttgaacttg accgaaaaag aggttgtga
//
LOCUS       DACEUZ010000031        24371 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.031, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000031 DACEUZ010000000
VERSION     DACEUZ010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24371
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.031"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(75..1442)
                     /gene="nhaC"
                     /locus_tag="IXG44_001061"
     CDS             complement(75..1442)
                     /gene="nhaC"
                     /locus_tag="IXG44_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAQ1657794.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(1551..2972)
                     /locus_tag="IXG44_001062"
     CDS             complement(1551..2972)
                     /locus_tag="IXG44_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1657795.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(3215..5092)
                     /gene="tdc"
                     /locus_tag="IXG44_001063"
     CDS             complement(3215..5092)
                     /gene="tdc"
                     /locus_tag="IXG44_001063"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAQ1657796.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(5383..6639)
                     /locus_tag="IXG44_001064"
     CDS             complement(5383..6639)
                     /locus_tag="IXG44_001064"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HAQ1657797.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     gene            7219..8520
                     /locus_tag="IXG44_001065"
     CDS             7219..8520
                     /locus_tag="IXG44_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAQ1657798.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(8589..9188)
                     /locus_tag="IXG44_001066"
     CDS             complement(8589..9188)
                     /locus_tag="IXG44_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1657799.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            9442..10323
                     /locus_tag="IXG44_001067"
     CDS             9442..10323
                     /locus_tag="IXG44_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1657800.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            10336..11945
                     /locus_tag="IXG44_001068"
                     /pseudo
     CDS             10336..11945
                     /locus_tag="IXG44_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
     gene            complement(12022..12681)
                     /locus_tag="IXG44_001069"
     CDS             complement(12022..12681)
                     /locus_tag="IXG44_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAQ1657801.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(12964..13629)
                     /locus_tag="IXG44_001070"
     CDS             complement(12964..13629)
                     /locus_tag="IXG44_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1657802.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(13776..14381)
                     /gene="dhaL"
                     /locus_tag="IXG44_001071"
     CDS             complement(13776..14381)
                     /gene="dhaL"
                     /locus_tag="IXG44_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HAQ1657803.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(14409..15392)
                     /gene="dhaK"
                     /locus_tag="IXG44_001072"
     CDS             complement(14409..15392)
                     /gene="dhaK"
                     /locus_tag="IXG44_001072"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HAQ1657804.1"
                     /translation="MKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRVVAKNQKAEQ
                     VGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEADSGKGVLLIV
                     KNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAGTVLVHKIVG
                     DAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDEIEYGVGIHG
                     EPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQFVFMNDVLA
                     LLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTISW"
     gene            complement(15394..15789)
                     /gene="dhaM"
                     /locus_tag="IXG44_001073"
     CDS             complement(15394..15789)
                     /gene="dhaM"
                     /locus_tag="IXG44_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HAQ1657805.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            15943..16263
                     /locus_tag="IXG44_001074"
     CDS             15943..16263
                     /locus_tag="IXG44_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HAQ1657806.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(16375..17244)
                     /locus_tag="IXG44_001075"
     CDS             complement(16375..17244)
                     /locus_tag="IXG44_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ1657807.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(17340..18644)
                     /locus_tag="IXG44_001076"
     CDS             complement(17340..18644)
                     /locus_tag="IXG44_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ1657808.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFI
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(18641..19150)
                     /locus_tag="IXG44_001077"
     CDS             complement(18641..19150)
                     /locus_tag="IXG44_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1657809.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(19325..21460)
                     /locus_tag="IXG44_001078"
     CDS             complement(19325..21460)
                     /locus_tag="IXG44_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ1657810.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(21543..23198)
                     /locus_tag="IXG44_001079"
     CDS             complement(21543..23198)
                     /locus_tag="IXG44_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ1657811.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKKLDGAFSLYQTVRPEELIYPNK"
     gene            complement(23200..23514)
                     /locus_tag="IXG44_001080"
     CDS             complement(23200..23514)
                     /locus_tag="IXG44_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAQ1657812.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            23684..23944
                     /locus_tag="IXG44_001081"
     CDS             23684..23944
                     /locus_tag="IXG44_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HAQ1657813.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            23958..24272
                     /locus_tag="IXG44_001082"
     CDS             23958..24272
                     /locus_tag="IXG44_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur reduction protein DsrE"
                     /protein_id="HAQ1657814.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
BASE COUNT         6944 a         5036 c         4396 g         7995 t
ORIGIN      
        1 aaaagcaatt ttcttgagtt tcttcttatt tcttggagtt aaacgcttct gtcccagcct
       61 cttttttcct tccttcaatt ttttacacgt cgacgattca ataaaaaacc gcctaatatt
      121 gtaaagacag gtgcaagaat cgagaaaaag gcgaatggca agtaatgcaa cgcgctaact
      181 ttcatcgttc ccataatgaa tgtgccgctg actccccacg gtacgagaga attgacggtc
      241 gcacctgaat ccgctagtgt tcgtgtcaaa taggtttttg ataagccaac gtcatcaaaa
      301 gaacgcttga atgtttctcc tggaagaatg atggacagat actgttctcc aacgatcaga
      361 ttgattccga tacagctcaa ggcagtaaac ccgactaaac gagaaggtcg gtccatcttt
      421 tcttttaatt cttgaacgat ggtatcaacg atcagatatt tgatcagtaa cccacctaat
      481 gccaatgcaa gtaaaatcaa actagcagaa cctaacatac tcatgatccc gcctcgagaa
      541 agcagttcat ctagctttgg atcaggtgta tttgcgacat aaccattcat caagatgtct
      601 gctgcttttc cgaccgtcaa acttttctca tggataaaag aaagaacgag agcagctgtc
      661 gatcccgcta gtaatgtagg gatagccgga atttttttga ttgccccccc aaataaaata
      721 actagtggaa tcaatgtcca aggagagatc caaaagctcg ctttcatgtt ttggatcatt
      781 tcattgatac tagctagatc ggctgaatga ctgttatgtc ctaatatcgt ataaaaaatc
      841 aacgaaagga cgaatgctgg gaaatccgta taacgcatac cagcgatatg ttcgaataga
      901 ttaactcctc caatacccgc tgccaagttg gtcgtatctg ataaaggcga gcagttattc
      961 cctaaaaagg caccggaaac gactgcgcct gtagtcattg catcattgaa tccaagtaaa
     1021 tgaccgatcc ccaaaaaagc aattccaatc gtcgaaatcg ttgtaaatga actgccgaca
     1081 gtcactccga caattgcaca aacaagaaat gctgtcggta aaaagaaacg cacagaaaca
     1141 agtttaaatc cataaaccat aatcgtaggg atggttccag ataaaatcca tgaagcaact
     1201 aagacaccaa tcaaaagaaa aatcaggatt ggaatgatcc ctggagtgat tcctttgatg
     1261 atcccgtcat ggatctcatc ccatgtgaac tttttaaatc tgccataaaa caacaaacac
     1321 atgaaagctg caaggatcgg gatatgtggc gagagctgga atccgatgat cagtactcct
     1381 aggagtgcca acaacacgat caatacagcg atactttctt ttaaagaaat atttttattc
     1441 atcgtcgatc tcctatttca aatgaaaagg ccctgccatg aaaaaactat tcacttggca
     1501 ggacctccta ctaaatcagc gacaaaacgt cggctgaaag acttattttt ttatttttct
     1561 tctaaacttt tttctacctc aatcccccaa gaatctttct tgaagtgatt gatgatcaat
     1621 ggaataacga acatgaccac tgtgatagct gctactaata atgcatcact caatgtcgct
     1681 gtacctaatg tagcaaatac gacaacgatg atagagaaga tcagcatgca gctgactacc
     1741 caagctaatc cgtttccgtt tttaccaatt cggtaaggac gttcgaattc tggatgtttc
     1801 ttacgcattt taagcaatga aacagcaatc aaaatgtaca cgatacaata aataacagtt
     1861 gtagcatttg taagtgtcaa gaatacagca cttacgtcat ccattacacc ataaagcaaa
     1921 gcaaataatg aaataacgat tgattgtgtt agaacgacat ttctagatac gccgtatttg
     1981 ttttctttat ggaatccgaa ttttggtgga aggaatcctt cacgagctac ttggatgatt
     2041 gtttttgatg gtccagtaac ccaagctgat aattgtaaaa gcacaccaat aaataccatg
     2101 aagctgaaga tatttccgat cacttctggt aagcctagga tttgacagtc aatcaagata
     2161 ggctgcgtga tatttgctaa ttccattttt ccatctggta cgacatttgc tacaagcatc
     2221 gcattgatga cattcaataa aactaatccg atcaaagcga taaatacgcc ttttgtataa
     2281 ttttttgttg cgtctttcaa acgaggcatg taaacagaac tcatttcgat cccaacaaaa
     2341 ataaatgtga taccagctaa gtatttcaat gaatcaatat gttccaagtc agggaaagct
     2401 tttgaccatg aaaagtcacc tagatagccg tttgagacta gtccgacttt gaaggctgcc
     2461 aaaacaccta aaacgaacat gatgactact gggatatata cccctagcca tacaccgatg
     2521 ttcccgccga cttttgccat atcgaatttc aagttcaaga tcgtgatgat ccagtagata
     2581 actaaaatac aacctaaaac gaaccagtga ttattgccaa gttctacatt accaaatgtg
     2641 tttcctaaca atggtcctaa agtagaagcg accattacca tacctgggaa catttgcacc
     2701 cataatagcc atgcaacgac aaatccccat ttgctgccca gtgctgtttt tacccataac
     2761 tgaggaccgc cttcttgagg aagcatcgtg gaaagctcac cggaaaccat tgatatcgga
     2821 ccggcaaaaa acaaaactgc aaaaattgaa taaaaaatca atgtccaacc gactgcggct
     2881 aacgtaggaa tactccgaac cgttgcacac agtgccattg tcagaccgat aaaggtccca
     2941 agacccattt tacttacttc agatttactc accgttgaca ctctccttga ttttttggat
     3001 aaaataacgg tgtctttgaa gtagtttcag caataaacga aacaattcag cgaatatttg
     3061 aggggattct ttgagtttat tgcttgaagg gtttcttcaa agacaacctc aaaacaaagg
     3121 gatcgaagag cttaggtctc cccttgctct tcagatccaa acatgcgtca gaaacagtta
     3181 gtattcaagc tgttttctaa catgattacc ttgcttattt tgcttcgctt gccaataatt
     3241 ggtctgcgta gattttttcc aatttttctt gaagagctgc tttgattttt tcagcatatt
     3301 cttcaaagtg ttcttctttg ttcatgtatg gtgtcattac gctggcacgc aatacagtta
     3361 cttttccggc acggttccat tcttcatctg agaatcctag ttggttcaca aattgtaatg
     3421 ggctgttgcc atagtctggg atagcaaagt cagtatgtga agttaagaac tcattaccgt
     3481 aaatgcttcc tttcacgtat gaagaataat catatacgtc atggttcaat ttattcattg
     3541 caactaagtc atcgttgcct ttttctttga atacatagtc taccatattg aaatctggat
     3601 aagttaatgg atgaacttcg atttctttgt ctcctacttt aaatgacaag tcattcaaga
     3661 agttgtagaa acgatgtgca ccttcgattg atgcacccat taatttacca tatcctgtga
     3721 cattcaaagg taagacatgg tgtgctgccc aaacgctggc tgctgttgcc cctgcttttg
     3781 aaccttccaa gatataagcc ccaagtaatg ctgggatatc tgcgcctttt tcaaatacat
     3841 atgtcgcaaa gtaagagata acgtctctca tgcggatatc tttgatgacg ataccaccgg
     3901 cagaatatgg aacatagccc attttatgtg ggtcaatcgt tacagattct gcttcttcga
     3961 ttgctttata tgcactgtgt acttcttcta agatataatc tttgttttct gtaaagacat
     4021 tgtatttgta atgaacatct tttaaatctt caaatggaat gaagttgttg tcttcgtcta
     4081 agaaaatcgc acggccgtat ccgccataag cagcatctac gtgtaagtag aagtaaatac
     4141 cgtctttttc taatacgcga cgcaatgcaa cgattttatc gataccgtcg attgctcctt
     4201 cttctgttga tcctacaaca ccgactacac ctaggattgg tgttttttca gctgctagac
     4261 cacgaacgat tttttcaagt tcgttgatgt ccatgcgata gttgtggtca actggcactg
     4321 gaatcacttg gtctaaaccg ataccgatga tgtcggcagc ttttagccat gaatagtgtt
     4381 tagtttgtgg aaccaaccat tttcctaatt tttctaaatg ttttccgcta cgtgctgagt
     4441 gagctttgat ttcgtcgatt ttttctggga cgctgtctaa cagattcatg atttcttctg
     4501 ttgacaagtt cattaattcc caatcgcttt ttccagcaac tagttcaggt gtcacttctt
     4561 tcattgcaag cggcaatgat ttgatgttgc gggcatacca aagtccttct aagtttgcta
     4621 aagaaccatc agcaacgatg tggccccagc catctttata gctcattaat tttgcaaatt
     4681 ccattcctac ttcttcttcc atttggcttg ttgcaggaga agattcatag gcaacgttat
     4741 taccattcca tagcatagca aaattgtacg ctagtaaaga tggcatcaat gtttcagagt
     4801 tcatatgacc ccagtaacgt ccggcattat gccaaggaac ggaatgggta cgcatccgcg
     4861 ctgaaatttc tgatagaaca tcctttgttt tattcacagt gtgttcaaaa ctagcactgc
     4921 ttttttcttc tggcgtaata ataggcatgt cttgaggcat gtagttttga cgccagccta
     4981 aatgttcatc caccaattca ttcaacaaag ctttatagat ttggccgttc tcagctttgt
     5041 ccccaatgaa tagggcattt aggtttagat ctttcgacaa tgattcactc atagtgaaac
     5101 cctcctaaaa aaatatttaa attttgtcga gccctacatt acacctgttc ttctattctg
     5161 taaactaagt tttggcctga tttttaatca tattttaata tgtatcaatt atccgaattg
     5221 ttcacacttt tgaaataatt aaaaaaaacg agggaattta caaatgtaaa ctccctcgca
     5281 aagattgtat taacgacaac cagcgttcta ttttaggatg ggtttcaaaa ttggaatgtt
     5341 gtaatagtat ttcccttttt caaggtaaaa acttactaaa ttctaatcaa ttactttggc
     5401 caaaaagtaa tttttcttac cttttcgaat aaccacaaac ttaccatcga attcatcgga
     5461 tggatttatg acaaaattta aatcggtaac gcggtcaccg ttaatactta tagctccgtt
     5521 tgaaacatct tcacgtgctt gacgtttaga tggttcaatt tttgtggaaa caagcagttc
     5581 cacgatattt tcaggtgtgc ttgaaatttc aacgttcggc attttaccga atccttgcgc
     5641 gatttcttcc gcatttagtt ctttaatatt tccagagaac aatgcttgcg ttattttttg
     5701 cgcttctttc aagtcttctt cactatgcac aaatcttgta acttcttctg ccaaacggcg
     5761 ttgtgcttca cgtttctcag gttctgtctc tacttttttc gctaagtctt cgatttcttc
     5821 ttgagacaag aaagtaaaga atttcaagta tttcactaca tcgcgatcat cttgattcaa
     5881 ccagaattgg tagaactcaa atggtgaagt tttcttcgga tcaagccaaa cggcaccgcc
     5941 tgcagttttt ccgaatttcg ttccgtcagc cttcagcatc aatgggatcg ttaaaccgaa
     6001 cgcttctgct tctggtcctt ctaattttcg gattaggtct aatccagctg tgatatttcc
     6061 ccattggtct gctccaccga tttgcaattg cacatcgtat gttttatgca gatgcaaaaa
     6121 gtcgatagat tgcaggattt ggtaagtaaa ctctgtaaat gagattccta cttccaaacg
     6181 gctggcaacg atatcttttg ctaacatcgt gttgatgttg aagtttttac cgtagtctct
     6241 caaaaattca agtaaagaga tttttgacaa ccagtcgtag ttgttcacaa atgtgatatt
     6301 tgcatcttta ccaaacaatt ttctcatttg attggaaagt gcgtccacgt tatgttgcac
     6361 ttgttccatc gtttgcaaca cacgttctgt tttgcggccg cttgggtcgc cgatcgttcc
     6421 tgttccgccg ccaatcagaa tatatggatg atggcctgcc aattggaatc ttttcatcat
     6481 cataaatgga atcaaatgtc cgatatgcat cgaatcacca gttggatcaa cgccgcaata
     6541 aagtgaaatg ctcttttctt caacgagctc gcgaagtctt tcttcgtttg tttgctgatt
     6601 aatggcgtcg cgccaagtta attcatcaat aatattcatt ttactgacct ccgtgtagtt
     6661 tttttatgaa agcaggaacc tgctagcatg tgtaattaat tatttcttca ggataggata
     6721 ggaaataaaa aaagtccctt ggcatataag ccaagggacg aaatctcgcg gtaccaccca
     6781 atttgtatgt ctagatatcc aacatacctc tcgaacattt aacgctgtcg cgtggcaatc
     6841 actgccaaag actcctgaaa tgtaattcgc tttaaagcat gtactggttt gcagcgacca
     6901 ccagctttct gaaacattgg ctttaacaac tacttcgttt cgatcttcgt cttgttatta
     6961 ttaatatgtg tctatcatag agcgcaactt tttctttgtc aacagaaaaa ggatattttt
     7021 tttaaattcc tcattttttc ttttttgaat ccgctctaat tccgaacatt ttattttcaa
     7081 ttattgttaa aagaaataac tttttctgta ttttttctgc aatttatcga gaagtcactt
     7141 tactttgcct cttattcttt cttcgttaca ataaggaatg ggacaagtaa aaaaatgaaa
     7201 aagcaaggga cgaggtatat ggaaaaaaag caaaaaatta ttttaggagc cacttctgct
     7261 ttaactatcg ttattttaat tctaagtttg ttttttgtca tccgatcatc tacaaaaaaa
     7321 gaaccaatca ttcctgaaaa aaaaataaca gaaaaagcaa aagaaccgac agatgataag
     7381 caacaactag ttactcaagc aaaaaatctg gctcaaagct acgattatga ccaagcgatt
     7441 gcgttattgg aaaaagatag taagaacgga tctactgaaa cacaacagct tcttcaaaca
     7501 ctaaaaaagg agaaagaaca actgatcgac tggtctgatc ctactcagat ctctcatgtc
     7561 tttttccaca gcttgatcgt ggacccgcaa aaagccttcc actctcaaca ggcacaagga
     7621 tacaaagatt atatggtgac tgttgaagaa ttcaaccgtt cgatcgaaca actttatcaa
     7681 aatgattttg ttttagttaa tttgaatgat ttgatccaaa aagacgaaca aggaaatctt
     7741 tcctttaacg ggcttgcgct acctgaagga aaaaaaccat tggttctttc acaagatgat
     7801 gtcagctatt atgaatatat ggataattcc ggttttccta gccgattggt cgtatcaaat
     7861 aacaatgaaa tcaaaaatgt ctataaagag aaagataaag aagaaatcgg tgactttgac
     7921 atggttcctc tgatcgatgc ctttgtcaag aaacatcctg atttttctta tcgaggcgca
     7981 aaaggtacct tggccctgac aggttacaac ggagttttag gttatcgaac atcaaaatct
     8041 caatatggag acaatgaaaa aacaaaaaaa gagattaaag aagctaaaaa agtggcggaa
     8101 cagttgaaga aagacggctg gagctttgct tcccatacat ggggtcactt gaatatgact
     8161 caagcttcac tagaagatgt aaaaaaagac aatgaattgt ggcagcaaga agttgcacct
     8221 atcctaggaa aaacaaatat tctgatttat ccatttggtg cagacatcag cgactggcag
     8281 ccttattcag cagaaaatca aaaatttgct tacttgaaga gtcaaggttt cgatattttc
     8341 tgtaatgtcg atgcttctac accagcttgg ggacaattag gtacggattt ctatcgaaat
     8401 gctcggatca atatcgatgg cattcgtttt caatctgatt tagacggaaa aaatcctatt
     8461 cttgatccat tcatcaatgt ccatgatgtc tatgacaaag aagcacgcga atctgcttga
     8521 ttttacgtca tttatgaacc aaaaaagccg aactgacatt ttatcgtcag tttcggcttt
     8581 tttatgcttt atttcttctt cggtgcgatt cccttgatca ggacttctgt ccattctgct
     8641 gtatatggaa gcagttttgc gtatatctcc ggcttagaaa ttccttctac cattgcttga
     8701 aaatagagga tcaagaactc atccgaatat ttcaaatcca cttttccttc tttccgccct
     8761 cgatggaaaa gatcaagcat gatactgaaa ctttcttgcg tatacgcatc catcgcgtca
     8821 gaaaattctt ttttttcttt ttttgtatag tatgtcatca aatcgatata aaattgtttg
     8881 ttcactcgat tgagataacg gattttgttt tcactcatgg caatcagtgt ttcttcaaat
     8941 ggaagatttt ccgcaatcaa cgctttcgct tcttgcccta aatcattcat aaatgcaaaa
     9001 aatacttcat aaatcagccc ttccttgctg ccaaaatatt taaaaatcgt cgctttactt
     9061 acctgagcgt tctcagctac cttatccatt gtcactccag cggttcctat gtctgtgttc
     9121 atcaagtcaa aagctgcttg taaaatctct ttctttttct cttgtgctct cttttcaaaa
     9181 ccgttcattt gtagagcctc ccgtaatttt aaacttttat aatatatata gttcaaattt
     9241 attctgaggt ttattataac tatctattga gaaagtatca agctagcaat tccttgttta
     9301 tgaattcatt gacagaaatt tataaaaaga atattatgta ctaaaataat aaaaaaagtt
     9361 ttataaaaag gtataaaacc tttttattaa actatttaat cttgtttagt atatattttt
     9421 agaaaaagga gtggggtttt tatggcagaa atcgtcaaag tcacacattt gaaaaagagc
     9481 tttggtcgtt tcgaagcttt aaaagatgtc tctttcaccg tacattcagg tgaagtcgtt
     9541 ggatttatcg gaccaaatgg ttctggaaaa tccacgacga ttcggacact tttagggatt
     9601 ataaaaaaag acggcggaac ggcaacgatt tttgggcaag atgtatggga agatagttta
     9661 tctatccatg aacgtctttc ttatgttcca ggcgatatct ctttgtgggg aaatttgact
     9721 ggaggagcga tcatcgatct cttcatgaag cttcatggaa aagggagtgc tcaaaaaaaa
     9781 gatgaactga ttcaacgttt ctctcttgat cctaagaaaa aagcaaaaaa ctattcaaaa
     9841 ggaaatcgcc aaaaagtggg cttgattgct gctttggctg ttgattcgga tctctattta
     9901 tttgatgagc ctacttctgg tttagatcct ttgatggaaa cgattttcca agaagaagtt
     9961 gaaaaattga aagaacaggg caaatccatc ctactttctt ctcatattct cagtgaagtc
    10021 gagcgactag cggataaagt cgtgatcata cgcgaaggaa cactcgatga agaaggaaca
    10081 ctcgatgaat tgcgtcattt gactcgttcg accatcacta tagaaacaga agcagagaat
    10141 agtcacttag cacaattgaa cggtgtccat gactttgtgc aaaaaggtca cctcgctcaa
    10201 tttgctgctg ataacgaagc tttcgatgtg attttagctg aagcgtccaa attaggtgtg
    10261 aaaaaatttg aatctattcc accgactctt gaggatttat tcatgagaca ttatgaagga
    10321 tgaggaggaa gcgaaatgac tgataaattt tcacgctggt ccaccctttt ttctcaaaac
    10381 cttagccgtg actggaaaaa gatcctgata tggctagtcg gtgtcactgc tttttctggt
    10441 ggttttgtcc ctgcgtttga agaattgact tccggacaag gattagctgg attatacgaa
    10501 accttgcaaa acccggcaat gattgcaatg gtcggaccaa ctccagcttc gaatgctgct
    10561 gagtatactt taggagcgat gtatgcaaat gaaatgttgc ttttttgcag tttgtttgct
    10621 gctgtgatct ctgttttaca cgtggtgagc caaacgagaa aaaaagaaga acttggtctg
    10681 attgaactta ttcgttcctt tcgagtcgga cgtcaagcaa actctttagc tgtcatcgct
    10741 gaaagtattc tgatcaattt gcttttagca atatcgatca gttgtacgat gatttttttc
    10801 caagcggatt cgatcactgc agaaggtgct tggctttttg ggctatctgt aggtagtatg
    10861 gggattttgg ggagtgccgt tgcactgttt gctgcacaac tgatgccaag ttcagccagt
    10921 tcgaatggtc tgtctttagc gtttttaggt ggactttacc ttctacgagc aataacagat
    10981 gtagctgatt cctccctttc gatgtggaaa cctttaggct ggtcttattt gacctatcca
    11041 tttacaacta acaactggat tcctttgctt tatttgtttt tgttcagtct aatcttgata
    11101 atcagttcat ttgttttaga ggaacatcga gatttgaaca ccggctatat ccccgaacgt
    11161 gaaggaaaac cgtatgctaa acgttctctt ttatccttgc gtggtttatt attctatcta
    11221 aataaaggga tgacaatcag ttggcttatc acttttggtt tgatgggggc ggcatacggc
    11281 tctatctacg ggaatatgca acagttttta gaaagtaacg acttaatgaa acagatgttt
    11341 atccaatctg gcgtctcaat taaaaaaact ttacaagcac gatcatggtc gtcttgattt
    11401 gtctagctgc tattttacca attgctagca tcaatcgttt atttacagaa gaaacccacg
    11461 gacgtatgaa tcagctgttc gcaacaaaaa tttctaggaa ccaactttat tggaccaata
    11521 tgttactagc tattctattt gctatattgg caatcctgat tagttctggt gcactagggc
    11581 tttctgcttt ggcttcgatg aaggatgcga aagacatgga tctattgatg tttcttaaag
    11641 ccgggttcaa tttcctgcct gccgttttag tgtttatcgg aattagtggc tttctattcg
    11701 gatggcttcc tcgaatcggt aaacttgctt atatctactt agcttactcc tttgcactca
    11761 attactttgg cggaattttg gatttaccag aatggttttc caaaacagct gtccctagct
    11821 gggttccacg gatgccaaac gattcttttg atctttccgt gttccttacg ttatcatgta
    11881 tcagccttgc actgttcgtc cttggttcta ttggttatag gcgaagagac ttactagaag
    11941 gataataaaa aagcatgtac aatttcatgc tagcctccaa atctgggtca aaaatccaac
    12001 atttggaggt cattttttta tttacaagtc ttctaactgc tctttatcaa tgatccagac
    12061 ttctcgattc tttcttttta cataaccatt ctcctctaat aatttaaatt ttctagataa
    12121 agtttcgggg gtcgtaccga taaatgcagc aagttctttc attttcattg gcaggaaaaa
    12181 gtgatccttc cctgtcgctt tagctaagtc aagcagatag acagcgactc gttcttctac
    12241 tttttccatc atcaaaaaag ttgtctgctg ctgtactttt gttaatttct caacgttgat
    12301 tgctaaaagt ttcagactaa gggcgggata atgcagtaag agttccgaga aatctttttg
    12361 tctcaatatg caaatttccg ttttttctag tgcttcgcca aataaagact catttggaac
    12421 gcctagcaac tggctctctc cctcatatcc gcccggctct attactcgta aaagctgttc
    12481 ctttccgcta gcagataatt gatacacttt catactgccg gaggccacga tcactaattg
    12541 aggttctcca ttcggcgcaa gaatctgttc tcctttttcc acatagtgat gtttgacaag
    12601 tgcgtgtact ttttcttgat cttgctgtac taagtgattg aacaaaggaa caaatgaaac
    12661 acataaatga tcatgactca taatctctcc tccttctaaa cagctatttc taataaagaa
    12721 acataacttc ccttattcca actgataatt ttcttttatc atagcataaa cgtcaagcga
    12781 accagatacc agcaaataga gtggatttct ttttgtccct tgctttattt tccttctttt
    12841 gagaccttaa gtaattgatt cctatcaatt ttttaaatat ttactttttg aataatcaaa
    12901 aacaaacctt cactcgcaag ccgagagtaa ggtttgtttt tcgcattgct tatttcgtgt
    12961 tgtctattga aatttttccg cacctagctt ttgatatagt ggtgctagtt cttgtaaggt
    13021 ttcagcggtt atttgctcaa cgtgttcgat ttcttgcgga tagaagaata gtttacccat
    13081 ttgaaaggtt cggtctctca tcgctttttt caactcttct tgtttctttg tatcggacaa
    13141 atagtcagac aaagctaagt ggtcgtcaag ttcatgttct ggttgcggcc atatgtaata
    13201 atccttgata tcgactgttt tgctccactc tttcagtatc gcaagcaact ggttatacgc
    13261 tgcaatgcta ccatatcgtt cttcgctttt ataatgttgg tacatcaaat agatttggta
    13321 ttgtttttga ttcaacaaaa ctgctagaca ggcattctcg tcttgacgag attcgcttcg
    13381 gtacgcgctc caaaaatgac tgcgtaaatt ccagccattc gtccagcttt cgattttagg
    13441 tttgctcatg ccgtacatat caggcaaaag tgcagctgtc tggagctgaa tctctttcca
    13501 tttttgccag tgttctttat attctttctt gatttgttca atcttatctg ttggatggct
    13561 ctctttgagc tgtttgaatg cgtagattgg tcgatcaaac aactgataat ctgattttcc
    13621 aagttccatt gtttcgcctc tttccgttat cttttgtatt ctacctgaat tttgcgatat
    13681 ggacaaatag aggttgtgaa aaagctgttc tgcatcaagt cataagaaca gccaaacaaa
    13741 aaactgcgat gagcatcacc gcagttttat tgaacttaag gttgattgat cacatctaaa
    13801 agcgttgaaa ataaaatggc actagaagca gctcctgggt cgatatgtcc gattgcacgc
    13861 tctcctagat aggaagctcg tcctttttta gctaccaaga ctttggtgtg ttcttttgcc
    13921 tggtcaatga tttcttttgt taaattccct gccttcaatg cctctgtgac tggcagccaa
    13981 acatctaaca tcgttttatc gtcagcttct gcctttcctc ggctttgaat acccgctgtt
    14041 ccctgctcga tcacttcgcc taatttttct tgagaatcaa tagaatcgac ctcttttgtc
    14101 gctttcatca tgttcatgaa tgcagatccg tataacggac cagaagcccc accgactttc
    14161 gatatcatcg ccatagagaa tactttgaat gtatcgctga ttgtttcagg atgatcggct
    14221 tgaaaagctt cttcataagc tgaaacccct cttcccatgt tatttccatg gtctccatcg
    14281 ccaatcgggg tatctagatc actgagatat tgtttgttct gattgattgc ctctgcaaat
    14341 tgcgtgagcc attcttggat ttcttgaacg gttagtttca tattatcacc cttttatctt
    14401 gagatttttt accatgaaat cgtctgtaca ttgctttcta aagaatcttt ccattgtgtc
    14461 tccgtcaagt cgatcagtgt tagagaaaga ccttgcatat ctagtgaggt cataaaatta
    14521 ccgacttttc ggaaaactac attgacgcct ctttcttcca acagagctaa aacatcattc
    14581 atgaacacga actgttccat caaaggcgtc ccgcccattc cgttgactag tacacctgct
    14641 tcttttggta aattcgagta atcatctaaa atcttagtaa caagttcctt tgccagtact
    14701 ttagaaggct gcatcttttc tcgacgatag cctggctcac cgtggatacc aacaccatac
    14761 tcgatctcat cttctcctag ttcgaatcct ggtttcccta cttctggtac agttgctgct
    14821 cgaagcgcta gaccgatcgt cttagttgcc tgtacgacct tttctcccag ttctttcaac
    14881 tcagctaggg aagcaccgtt tcttgctgca tcgcccacga ttttgtgtac aaggacagta
    14941 cctgcaactc cgcgttttcc agcagtatac gtactatttt caacggcgat atcatcatct
    15001 acgatcacac tttctacttc aatatcgtcc atagcagcta attcctttgc catatcgaag
    15061 ttcaatgcat cccctgtata attcttaacg atcagcaaaa ctccttttcc actatcggct
    15121 tccttaatag ccgtctgaat ctgatctgga gttggagaag taaacacatc accaagcacc
    15181 gctgcactta acatcccttc ccctacaaac ccggcatggg atggttcatg tccgcttcct
    15241 ccaccagaaa ccaaaccaac ttgttccgct ttctgattct tggctacaac acgggaagat
    15301 tctacacgat gaacaagttc tggatagctt tttactaatc cttccagcat ttcttcgaca
    15361 atattgcttg gatcattcat gatttttttc aatttattcc catcctttct ttccggcatt
    15421 ttgtgcttct gttaagattt gctgagtatc atctgttata ccggcagtga tggctgctgc
    15481 aaaagctcct tccactaaag gcgcatccac cagatgataa cgttgctgct gctcttcttc
    15541 caacatgtct tttgccagtt ccgagttcaa taccgcgcta ccgatatccg caaaaacaaa
    15601 ataactagct gcatcgcttg attcattgac tgcttctacg attttcatcg ggtctgatcc
    15661 gatctcgcca tttattccgc cgagagaaaa aatttgcacc tcttcggatt tttgcatctg
    15721 ctcaatcatt tctttaatac catctgttac tttttgacta tgagaaataa gcaaaatact
    15781 ttttttcaca agaatcttcc tttcttttac atagttgcta acgattggtt tcgctttcat
    15841 taatgaacaa ttcaagtata taataaattt ctaattttgg gaaaatatgt gtagtgcttc
    15901 ttgaagtttg ttatacttta attaaaagag aggagtgatc gaatgattac aacaacaacg
    15961 aacagtgtag agggaaaagc cgtagtagct tataaaggga tcgtttttgg tgaggtcatt
    16021 actgggatcc atgcatttaa agacttggag gcggggttgc gaaatatctt tggcggccgc
    16081 tcaaaaagct atgaaaacga actaatgggt gcaagagaag aagcacttgc cgaaatggcc
    16141 gaacgtgcaa aagcattagg agctgatgcg atcattggcg taaaaatgga ctatgaagta
    16201 ttaggcagcg acaatggcat gctgatggtc acatgtagtg gaacagctgt ctccacgaga
    16261 taagtagggg acatctgttt taataactaa agagagatgg gacatgtagt ggcactcaag
    16321 aattagattt ttcagtctaa cttcgaggta ctctaccttc ccatcctctc ttttttattt
    16381 ctttttgcgg atgattccgt aagctgctgc gactaacaca cccgcccctg ctaaggaata
    16441 actgacagaa gctgtttttt catttgtttt tggcaaagtc tgacttgttg atttggatgg
    16501 ctctgtcgtt tgctgaactg tttctgtcgt gcttccagag gtcactgatt tttctcctgc
    16561 cgaatgcaaa atatctttta ttcgttggaa actagctgct aagtcagctt tttttgcttc
    16621 aaaatcatta gcaactgttt gtgcttcgct gatctttccg tggattgctt gactggccgc
    16681 tgtgatatcc gcttgagttt gacttagctt tccagaagct gtttgagcaa cctgatttaa
    16741 ttgctgattg aattcttttg cttgttggat cttcccgtca agatctgttt ggacagcttc
    16801 cgcttttgct ttcgcttcgg caagcttttt ctcaagttgt gcacggtcat ttcccacttt
    16861 atttttaaga tcagctaaat cttgatcgat tgctgcttgg tcttctttca atttgttttt
    16921 tgcagcttct aatgcttctt cgagttcttt tcttttattt tccaagtctt gcaaatgatc
    16981 gatttggtca gaagaggtag aactatttat tgatgtatct tctgaagatg aaatcgtttc
    17041 tcctgaatta tctgtcggta cattggaagt catttctgtt tccgaggcac cgttttcagc
    17101 accagaagag cttatggttg ttgaaggttc tgtttgagca ggttcagaag tttccgcaaa
    17161 agctgtctgt cccaataaga aaatactgga aacagcaatt ggccagatta acggcttcat
    17221 tctttttttc atcacttttc ccatccttac tactttttaa ctaatttctt attataagca
    17281 aaaatgtttg gaagaaccat aaacacaaca ggctgtttgt ctaaaaaacg gtgcatcttt
    17341 tatttttttg tacgttccgg gcttttccat gtcccgcttc cataacctaa aatcgttttg
    17401 actgctggaa tgaaagtcgt cacgatcgtt atcgcgttca ctatccagaa caaaagcata
    17461 tatagtggtg cgaaaagaag gtatttcaat tttcttcgtc gatcatctac aatcagcgaa
    17521 gctagcaact ggaaaaatcc tgcgaccatt tcaaagcaga tgaacacaaa actgaatgta
    17581 aaagtaatat aaatctgttc gtagtttcct tgatatacat aatgcccgat taatccagca
    17641 aaaagaacta cggaaataca aaagaatatt gaccagacaa tactcaatgt ttgatcaatg
    17701 aagatgatcg ttcttccaat atgtttaaaa ggatggagca tcactttttt gaaattcgtc
    17761 agccagactt ctgttccacc ttttgcccaa cgttttctgt gtcgatatag catcttcaac
    17821 gtcacaggaa cttccatgaa aaagataatc tcaggagcaa aaacagataa ccagtcattt
    17881 aactgatgat cccaagcaat actgatgtct tctgtcgctc gatcttgtct gaatccgccc
    17941 acatcgatca aggcttcttt tcgatacatc gtatttgctc cactgtacgc ataaagacca
    18001 ccgaaaacag ctgactgtgt tcgtttaatg atgcccacga tacttgagaa ttcaaccgtt
    18061 tgagattttg caatcaattt ggtacgattt tggacatcca tatttgcagt gaccgcagca
    18121 atatgtcttg caccagggcg aatgaaataa ttcacatacc taatcagtgc atctggctcc
    18181 ggtaccgtgt ctgcatcatt gcttaaaatc aatttccctt ttgcaaaggc aagtccgata
    18241 ttaaacgcgt gggcttttcc tttatttttt tcgatccgaa ccacacgaag attagcgtat
    18301 tttttcatca gtctagctaa tatttctggt gtttggtccg ttgaaccatc gtctgtcact
    18361 aatacctcat agttgtgata attcaatttc gtcatcaaat actcgattgt atattcgatc
    18421 acaatttctt cattatgggc tggcaccatg atcgtgatga acggttcgac actcaaggga
    18481 acatctaccc attctttttg cttatgtttg tatataaaca cataacacca gacaccaata
    18541 aaccatacaa atccgcctaa aatcggataa gaaacgagaa aaaaatacat aatagacaaa
    18601 caagtatcta agaataaatt acctgtatgg gtgattgaga tcatttcagt tccaccccat
    18661 tttctttata caaatctgca atgaatgtcg tatctagatt tttctcttcg gagacagaat
    18721 aaaaacggac cgtttctctc tcttcttttg ttccaaaacg ttccgtatac acttcttcaa
    18781 gtaattcttg tctttttttc aatttttcat cttcatattc aaaagattta tttacttttt
    18841 tgtatagatt tcgattgttc cacctagtca atacaaatga ggtaatcagg aaactgaaaa
    18901 aaatcaatac aaaaaagccg attaagaaat acagcatttt cttttctgat aaaaaagcgt
    18961 acaaggaaaa atccttggct ctttctggtc tcgttaatgg gaaaaagacc catataaaag
    19021 gaagaacaac gatcatccat cctaaaagag ccactaacgt ttgccaaatc tttaaccagt
    19081 aattaccttt tgaaaaatac ttatctttca ccaaaattga tgcaacttct tctcttgtta
    19141 cttttttcat atcctcacct cacgtttttt tgttacgacg taggagttta ccatacattt
    19201 catttctttt cacataaagt gttcctaaat ttttcctaag aaaaagaaca aaacatttct
    19261 catcagcaaa tgaaaaatat tttgttcttt ttatcagttg ttctttttat tttttatctc
    19321 ttaattatgt tgctctttcc aatacgacaa tgcttttcat tcctctagat atcgccgtgt
    19381 acatttgtct gcgtcttctt tgctcatctc catcgttttc aaatggatat aagattacat
    19441 gatcaaattc cagtcctttt gctagactca aaggaatgat ttggcagttt tctaatccat
    19501 atgaattttt tttaatcgat tcttgtagcg tttgggtttc ctcttctgtt tgggtgataa
    19561 ctgccgcttt ttctcctttt agagaaacca aaatctcagc tagtattttc aacaattctt
    19621 cagaggatcg aaaagagcgg tattcgggtt cttttccttg cggtcggatc gatacgatgt
    19681 cgatcttctc ttgatccaca gcataggttt tgaaaagctc tgtgattgct ccgctagaac
    19741 ggtaactatt tctaagatca taccgatgga tcggcagatg gtagtcgtcg aaacaacgca
    19801 taatatcagc aaaagtagtg gaagaattaa agattgcctg attctcgtca cctactaaag
    19861 taaagtgagt ttttggaaat aattcgatca atagacctaa ttgtgccagg gtataatctt
    19921 gtacttcatc aatcaataga tagcgaaatg ctggtacatt taccttttca acaagtaaat
    19981 gacgaatagc taatataatg actgcttcat caacagtacg tgtgggttgc caagcataag
    20041 ctgagccata tcgtttttca taaattcttt caaacaactg atgctcatcc acccatgcca
    20101 tttcttcgat ttgtctggtt atttttcgat attttttttg taaaagtttc cttgcataag
    20161 ctacaatact ttgttccgaa tcatcagaaa tcaatttacc gaataattcc aactgcctat
    20221 cttctgtcaa cgagagtacc tgattctgca cagcaggact ttttgcttgt ttcaaaagat
    20281 gattcttcca ttcgctcatc agtgcctttt tcgttgcttg aagcctatcg atcaatttag
    20341 gatgagcagg aactttctca tatatttttt cgatcgtttt tttagaaagg actgtttttc
    20401 catttttagt aagtccacgg ataaaattcg gatgatctaa aaacaaagga tctgattgtt
    20461 ttagattatc gataaacttt ttgctacgca aacgttctgt ctgctccgat acattttctc
    20521 ccgtgatacg tttgaaataa gcctcttcac cttctatttc ctctgctgaa agctgtgaaa
    20581 ccagctgtat gatggttaga ttcagtggat ttctttcacc taatgatggt aggacattcg
    20641 aaatatagtc gataaaccga tgattaggtg atagaatcag caagtcatcg gatgtcatcg
    20701 tatctcgata taaatagagc agatacgcaa tacgctgcat gatagcagag gttttaccac
    20761 ttcctgctac gccatttacc aagatatttt gacttttcac atcccgaatg attgtgtttt
    20821 gttccgattg gatcgtagca gtgatagcct tcatttcatt ggtctcatct tggcctaaga
    20881 cttctaataa tacgtcatct tgaatagcta cagacgtatc aaaaaacttg accaaacgat
    20941 ctctttccaa aagaaattgt cgtctttctt taataaccgt atcgatttta ttttgacgaa
    21001 ccgtataaga agatggacca atcatgttat tgtaaaaaag ttcggcaatt ggtgaacgcc
    21061 aatcataaac aagattttca tctgcatcat ctgtgaaatt tgctgtaccg atataaaagg
    21121 attcttttgg ctcattatcc agaaagtcta caacgacttt tccaaagtag ggacttttca
    21181 ataaacgttc gatctttgct agctgatttt ctgcgttttt ctgtttgata ttcatctgat
    21241 cgatttctct atttttcatt tcaatcatcg aaaaagtatc caggttatct aagtaactgt
    21301 caaaattcaa tcgaagctcg ccagccattt ctttcaacga ctggtttcct tctatttttg
    21361 ccgtatccat cgattcccgc agttcgtttt ttttatcaat tagcttttga tacacgttct
    21421 tcagatatgc ttcttctaac tgtttttgtg tgttgaccat acacattctc ctcttttttt
    21481 aaagtggtgg aaccgattag tttacctctt ttaagagtcg aatgcaagct ttttaacgag
    21541 tatcatttgt taggatatat cagttcttca ggtcggacag tttgatataa agaaaatgca
    21601 ccatcaagtt tttttacgtg gtagccagac tgcttcaaga ttctttccgc tagatagcta
    21661 cgtaatccgc tatgacagct gacgatatat tcttgttggc tgtctaattc atttaatcgg
    21721 tcccgaagtt cattcaaagg gatatgtttg gcttccttga atttgccctt ttccaattct
    21781 tctgctgttc gcacatctaa taaaattttt ccttttgcta gttctgtcgg caattcatgc
    21841 cattgtacgg tttcactaag cccttctgca atgttcattg ctgcatatcc tagcatgttt
    21901 actggatctt tggctgagcc aaatggcggt gcatacgtca attctaattc cggtaaatcg
    21961 aagatcgtca aatgcccttt gattgctgtt gctaaaatat cgatccgttt atccacacct
    22021 tttgccccaa caccttgtgc accataaatc tcgccagtct ctggatggaa gatcaacttc
    22081 agtaaaatat ctgttgcttc tggataatag cttgcatgat cttttcctga tacatgaatg
    22141 acttcaaaag acagttcgtt ttctttggct gttctttcac ttaaaccggt ggatgcggca
    22201 gtcattccaa acgttcgtat aattgctgtt ccaataccgc cttgatttct tcgagaaatt
    22261 ccggaaatcg tgtccgcaac ttgtctacct tgacgattgg ctggtgaagc aagagaaata
    22321 agtgcatctt ttccagtgat ttgctgtttt acaacgatag catcccccac cgcataaata
    22381 tcaggaatat tcgtttgata acgttcgtct actaggatac ccccgcgtat gcctaattcg
    22441 attccagcgg tcactgctag ctggttatca ggacgaacac caatggataa aataactaaa
    22501 tcagcaggga tggtacgccc atcatccagt cgcaatcgtt tcccgtggtc ttcaaatcca
    22561 gcgacagcat gagaagtaat cacttctact ccttgatgag acaattcttt ttctataaaa
    22621 gctgccattt cttcatccag tgttggaaga atatgtggtg cttgttcgat caccatgact
    22681 tctaaacctc ttctttttag gttttctgcc atttccaatc cgatgaatcc tgcaccaacg
    22741 atcacggcac gctttggctg tttttctaat gcatgcatca ctttatcgat atctggtaca
    22801 tttctaatag aaaaaacatt gtcggcttct gttatccctg gaatcgatgg aacaactggt
    22861 tttgcaccag gagataaaac caatgcatcg tactgttcaa tgtgtttttg accctcatgt
    22921 acgacttcta ccgttttgtt ttcgggaaag atggctgtca cttcatgatt tggacgcaca
    22981 tctaaacaaa aacgagcgga taatgattct ggcgtttgga caagaagatt ttcccgttca
    23041 ctgatttctc ccgaaagata ataaggcaag ccgcaatttg caaaagaaac atacggtcct
    23101 ttttcaaaaa caacgatttc tgcatcttcc attaatcggc gaagccgtgt agcagcagac
    23161 attccacctg ctacacctcc gacaataacg attttcatct tatttccctc ctactgttcg
    23221 atcaaaccat tgattcatgc caccacgtac attcactaca tcataaccag atttctttaa
    23281 ttctttagct gcttgtttac tcctcatccc tgattgacaa atcacataga cggtcttttc
    23341 tttgtctcca tcatatctgt ttatactttg taatggaaca tttttagctc cttttatatg
    23401 cccgccccgg tattctgcag gtgttctaac atcaagtaac gctggatttt ccaaaagctt
    23461 ttcctttaat tctgggactg ttatcgattt tattgattga aatagcaaat tcattttctg
    23521 cacctcattt acatttttat aaatacctca tggggtatat taaacaggag ctttattaat
    23581 atgtcaacca gaatgactct tcattttttt cactactttt tatatttgca taaaataccg
    23641 ataaggggta ttatctaatt ataaaaatag aggagaatgt gttatggaga ttgataataa
    23701 aaatatcctg aaccgtttga aacgtacaga gggacaaatt cgtggtatcc aaaaaatgat
    23761 cgaagacgaa aaagaatgta tggatgtcat cactcaatta agcgctgttc gttctagtat
    23821 tgatcgcgta atgggcatga tcgttgcaga taatctgaaa aattgctttg agaaacctga
    23881 tagtaatcca gaagaacaag cggctaaact agaacaagcg atcaagatga ttattaaaaa
    23941 gtaaaggaat gatttcaatg aaagtcgttt tccatattga tgaattagaa aaatggtcag
    24001 aaacagggaa aaacgtaaaa aacctgataa aagcttctcc agaaacgacg attgtcgtat
    24061 ctgtaaatgg catcgcgatt accggttatc tagactctgc caatgcagaa ttcttagatt
    24121 tgcaaggagt cgtttttcac gcttgcgcca atgctatgcg tgctaatcat atttcagaaa
    24181 gttctttacc tgaacaagta atcgttgtac cagcaggtgt ccttgattta gttgaactgc
    24241 aatcccaagg ttatgcttat atcaagcctt agtcagtaga ggttgcgaca gaagtgatca
    24301 gcttcaagaa atttcggctt atttttcgaa gaagctactt ctggaaaacc gtgaatcaaa
    24361 ggttgtgaaa a
//
LOCUS       DACEUZ010000032        23142 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.032, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000032 DACEUZ010000000
VERSION     DACEUZ010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23142
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.032"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            337..705
                     /locus_tag="IXG44_001083"
     CDS             337..705
                     /locus_tag="IXG44_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAQ1657815.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            885..1826
                     /locus_tag="IXG44_001084"
     CDS             885..1826
                     /locus_tag="IXG44_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HAQ1657816.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1895..2626
                     /locus_tag="IXG44_001085"
     CDS             1895..2626
                     /locus_tag="IXG44_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HAQ1657817.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2770..4950
                     /locus_tag="IXG44_001086"
     CDS             2770..4950
                     /locus_tag="IXG44_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HAQ1657818.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4970..5452
                     /locus_tag="IXG44_001087"
     CDS             4970..5452
                     /locus_tag="IXG44_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HAQ1657819.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            5468..6229
                     /locus_tag="IXG44_001088"
     CDS             5468..6229
                     /locus_tag="IXG44_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAQ1657820.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            6360..7694
                     /locus_tag="IXG44_001089"
     CDS             6360..7694
                     /locus_tag="IXG44_001089"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HAQ1657821.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            8001..8486
                     /locus_tag="IXG44_001090"
     CDS             8001..8486
                     /locus_tag="IXG44_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HAQ1657822.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            8479..9162
                     /locus_tag="IXG44_001091"
     CDS             8479..9162
                     /locus_tag="IXG44_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HAQ1657823.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            9288..11933
                     /gene="polA"
                     /locus_tag="IXG44_001092"
     CDS             9288..11933
                     /gene="polA"
                     /locus_tag="IXG44_001092"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HAQ1657824.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            11978..12814
                     /gene="mutM"
                     /locus_tag="IXG44_001093"
     CDS             11978..12814
                     /gene="mutM"
                     /locus_tag="IXG44_001093"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HAQ1657825.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12778..13407
                     /locus_tag="IXG44_001094"
     CDS             12778..13407
                     /locus_tag="IXG44_001094"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HAQ1657826.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            13459..13791
                     /locus_tag="IXG44_001095"
     CDS             13459..13791
                     /locus_tag="IXG44_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAQ1657827.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            14103..14594
                     /gene="nrdR"
                     /locus_tag="IXG44_001096"
     CDS             14103..14594
                     /gene="nrdR"
                     /locus_tag="IXG44_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HAQ1657828.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            14617..15990
                     /locus_tag="IXG44_001097"
     CDS             14617..15990
                     /locus_tag="IXG44_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication initiation and membrane attachment"
                     /protein_id="HAQ1657829.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            15990..16916
                     /gene="dnaI"
                     /locus_tag="IXG44_001098"
     CDS             15990..16916
                     /gene="dnaI"
                     /locus_tag="IXG44_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HAQ1657830.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            17045..17485
                     /locus_tag="IXG44_001099"
     CDS             17045..17485
                     /locus_tag="IXG44_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAQ1657831.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            17629..18330
                     /locus_tag="IXG44_001100"
     CDS             17629..18330
                     /locus_tag="IXG44_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HAQ1657832.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            18327..19448
                     /locus_tag="IXG44_001101"
     CDS             18327..19448
                     /locus_tag="IXG44_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HAQ1657833.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            19463..20695
                     /gene="pepT"
                     /locus_tag="IXG44_001102"
     CDS             19463..20695
                     /gene="pepT"
                     /locus_tag="IXG44_001102"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HAQ1657834.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            20827..21735
                     /locus_tag="IXG44_001103"
     CDS             20827..21735
                     /locus_tag="IXG44_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HAQ1657835.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            21770..22042
                     /locus_tag="IXG44_001104"
     CDS             21770..22042
                     /locus_tag="IXG44_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HAQ1657836.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            22053..23099
                     /locus_tag="IXG44_001105"
     CDS             22053..23099
                     /locus_tag="IXG44_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HAQ1657837.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
BASE COUNT         7801 a         4034 c         5068 g         6239 t
ORIGIN      
        1 cactctgagt cacaaagagc aatttcaaaa aatagcttct catgtttgtt gaatattgtg
       61 tcttgtgacc gaggagttgt caggagaaca ccgtttacca gaggttgtga cttgttttta
      121 gtcacgacct ctttttttat ttattccata taaatacaca ttatttattc cgaaaagtga
      181 aagcgcaaat aacaaaaaca accaacttat ttattattaa agaaaagcgt atttaagtag
      241 gacgattctg ttgagagctt tcgtagattt tcccattttc ctatggtaaa ctgacaaagt
      301 tgagaatgtt agaacagatc gtttaggagg aaacagatgt ttacgaatga aataaaaatc
      361 atgctttatg tggacgatgt agaaaaaaat gcaacgtttt ggagagcagt tggctttgcg
      421 gaaaaagatc gtcaagaaat ggatgggacg cttataattg aagtaggagt tgaggacagt
      481 caaacctcat ttctgttata tgatcgagca tttgttgagt cccattcacc tggcacagct
      541 actagtgccc catctttgat gttttacagt aaagatatct ttggactata taaaaaaatg
      601 cagacgcaat cagtcacatt aggtgatatg gtacagcttg gagaagaata tgtctttaac
      661 tttgctgatc ccgatggcaa ctactttgcg gttacaggta aataatagaa ataaagactt
      721 tgtttttctg tgcgaaacag gaaagcgaag tctttttaat tcaaataata atattatgta
      781 aacaaaaaac tttccatgcg taattataca caagcaaata aatcgtcaaa tgcgaacgcc
      841 ttctttatac tgtttaggaa cgaaaagaag gaaggaagaa atacatggat tatcaagtac
      901 tgctttatta caattataca aaaatcgaag aaccagagga atttgccaaa aatcatttag
      961 ccttttgcaa atctttggat cttaaaggtc gtattctagt agctgaagaa ggaataaatg
     1021 gtactgtgtc tggaaccgtt gaaaacacga ctgcctatat gaaagcaatg aaagctgatc
     1081 ctcgatttgc tgatacgatg ttcaagatcg atccaagcga aggacatgcc tttaagaaga
     1141 tgtttgtaag ataccgtcca gagcttgtaa gtttaaagct agaagaggac attgatccaa
     1201 accaagtgac aggtgcctat ctttcaccgc aagaatttaa agaagctatt ttagatgaac
     1261 agacagtggt gattgatgca cggaatgatt atgaatatga cttgggacat tttcgagggg
     1321 ctgtacgccc agatatacgc agttttcgag agcttccaga ctggatccgc gagcacaaag
     1381 aagaatttat ggaaaaacgg gtagtgacct actgtactgg tggcattcgt tgcgagaagt
     1441 tttctggttg gcttgtccgt gaaggattca aagatgtcgg acagctgcat ggcggtattg
     1501 ctacttatgg taaggatcct gaagtccaag gagaattatg ggacggcaag atgtatgtgt
     1561 ttgatgaacg gatcgctgtc gatatcaacc aagtagataa acaaatcgtc ggtcgcgact
     1621 ggtttgacgg aacaccatgt gaacgatata tcaattgtag caatcctgag tgcaatcgtc
     1681 agattcttgc ttccattgaa aatgaagaaa aacatttagg agcatgtagc atagagtgtg
     1741 cgaaacatcc gcgcaatcgc tatgttcaag accatggaat ctctccagaa gaagcagaac
     1801 tgcggtttac agctttaatt aaatagaaaa caatagtttt gattgtcgac catttccaat
     1861 atgcgctata ctagcgttga ggatggtgat ccacatgaaa tcattgattt atgctattgg
     1921 ggatgtccat ggcaaacatg agctttttga aaaacttatt cactattatg atccaaagat
     1981 ccatcagctt gttttattag gggatctcaa tgatcgaggt cctcgtacaa aagaatgttt
     2041 tttattagga aagcaattag tcgaagaaac aggagccatt tacctccggg gaaaccatga
     2101 agaatatttt ttgcaatttc tgcaatcacc ggaggactgg tatgcgcctt atatgagaaa
     2161 tggtggaaaa gaaacgtttg aaagcctgat tcacccaggg gcatcggagg aatactcccc
     2221 aactgaaatc tcgatgatga tccaatcgag atattcagaa ctgattaaat ttttagtcca
     2281 gcgccccttg tattatgaat gggaaaatta tctttttgtc catgccggtg tggatttgac
     2341 aaagaaagac tggcgtaaga caagtctaag agactttatt tggataaggg aaccatttca
     2401 tgaagggaaa aacaatacag gtaagacaat tgtatttgga catacgatta caccaatgct
     2461 ccatggagat atgcagacaa cagacctctg gattcaggat cataaaatcg gaatggacgg
     2521 tggggctgta ttcggagggt cattgcatgg ggttgttttt gacaaggaag gtattcttca
     2581 agatattgaa tatcagaaca acgaaggacc ttggcatgga atctgactaa tggaaagaat
     2641 gaaggagctg tgactttgtt gcggctcttt ttatttttat agagaatatt gtttctttca
     2701 gtagtttaac ccaggataaa aagtatgata gaataaagaa gcaattttta gcagaaaaga
     2761 ggcattttaa tgacggaaaa tttgaaccag acgatcattc agcttgtaca aaaagaactt
     2821 tcagactatc gtcctaagca gttgactact gtacttaatt tgttaaatga aggaaacact
     2881 gttcctttta tcgcgcgtta ccgaaaagaa atgactggaa gtcttgatga agtccagatt
     2941 cgtgaaatcg aagagcggta tgcctatctt gaaaacttag aaaaacgtaa aactgaagtg
     3001 attcggctaa tcgatgaaca agggaaactg actcccgagt tagaagcaga aatcacccaa
     3061 gccgtgaaaa tgcaacaagt ggaagatttg tatcgtccat acaaacaaaa acgacggaca
     3121 aaagcaacga ttgctaaaga aaaaggactt gaaccactcg cgaagtggct gatgcagctt
     3181 acagatggag aggtgcaatc tgaagcagaa aaatatattg acaaagaaaa agaagtctct
     3241 tctgccgaag aagcgttgca cggtgctcat gagattattg ctgaacaagt cagcgataat
     3301 gcaaaatttc ggacttggat tcggtcttat acgtataaca aaggtatgta tgtcagcaat
     3361 gtcaaggatg agcaagcaga tgaaaaaggc gtatatgaaa tgtattatga ctttgcagaa
     3421 cccgtccata aaatggtttc tcatcgaata ttagctacta atcgtggaga aaaagaagat
     3481 attctgaaag tattctttca agtagatgaa gcagcaattt tagcttactt ggatcgccaa
     3541 atagtcaaaa atcctgcctc tccttcttct tcattcgtca gagaagccta tcaggatagt
     3601 tataaacgct ttattcagcc agcaatcgag cgagaactgc gcaatgaatt gactgaaaag
     3661 gcagatgagc aagcaatcgc tattttcggc gaaaacctcc gtaatctttt gttgcaaccg
     3721 ccactaaaag gtaaagttgt cctgggattt gatccggctt atcgtactgg atgtaaacta
     3781 gcagtcgtag atgcgacagg gaaagtttta gcaatcgaag ttatttatcc tcataaacct
     3841 gcagcgcaag caaaaagaga agctgctgga ccagctttta tccaattaat taataaatat
     3901 caagtagata tggtagctat cggaaatggt acagccagtc gggaatctga actttttgtg
     3961 gctgagcagt tgaaatccgc cgaccataaa acctattacg ctatcgtcaa cgaagcaggc
     4021 gcttctgttt actccgctag cgaaattgca cgaaaagaat ttccgcatct acaagttgaa
     4081 gaaagaagtg ctgtttctat tgcccgtcgc ctgcaagatc ctttagctga attagtcaaa
     4141 atcgatccta aagctgtcgg agtaggccag taccaacatg acgtttctca aaaacgtttg
     4201 gctgaacaat tggattttgt tgtggaaaca gcggttaacc aagtaggtgt ggatgtgaat
     4261 acagcaagcc cgcagctctt gcagcatata tcaggattga ataaaacaac tgcccaaaac
     4321 attgtcattt atcgtgaaga aaacggagaa ttcaccgcac gaactcaatt gaaaaaagtg
     4381 ccacgcttag gaccaaaagc atatgaacaa gcaatcggat tcttgcgagt ccctggtggg
     4441 aaaaacattt tggacaacac aggtatccat cccgaaagtt attctattgc aaaagaaata
     4501 ttaacttcag tccaactttc agaaaaagag cttggtacag aagaagcagt agaaaaattg
     4561 acacgattat ccgtagagaa actggctgaa agcctatcag tcggagaaga aacgttagcc
     4621 gatattttag ctgggctgac acaacctgga cgagatatgc gtgatgagat gccagcgccc
     4681 ttattacgta cagatgtatt gagtatggaa gacttgaaac cagggatgga attgactgga
     4741 acggtccgta atgtcattga cttcggcgca tttgttgata tcggtgtgaa gcaagacgga
     4801 cttgtccata tatcgaaact cagcaaaaaa ttcgtcaagc atcctacaga tgtcgtgtca
     4861 gtaggagata tcgtaacagt ttggatagaa caagtcgata cgaaaaaagg cagaatcagt
     4921 ttgacgatgc tttcccctta tgaagagtaa caatgaggag taacaaaaat tgacacaaga
     4981 agaactgcag cagttagtag aagagatatc attaaaaagc ttccagcttc ctttcaagca
     5041 ccaagcaatg ttcaatccac gcttacgtac aactggcggg cgctatcatt taaacgatca
     5101 tcatttagat tttaatgcga agctatttga agagtcagac gaagagacga ttgcaggaat
     5161 catcaaacat gaactttgcc attatcatct tcatctagct ggaagagggt atcagcataa
     5221 agatatggat ttcaagtatc ttttgaaaaa aacaggaggg ttgcgctatg caccagcctt
     5281 gacgaagaaa cagataaaga tggagtatta tcgatgccaa gattgttctg ccacgatctg
     5341 ccgcaagaga agaatcgatg tacgcaaata tcgttgtgga agatgtcatg gagaaatcaa
     5401 atggattgag acaaaaagag aagactttaa tgaagcacaa aaaatcagat gaaaggatag
     5461 aaggcctttg gaaaaaagtg agcagaggaa tctaaaagac tggaatcagc tattaaattt
     5521 tttagccgca cctgaagagg gaaattttga ggaactgacg gcacctacac ttattttagc
     5581 tggaaactgt cttcctatct tagctgataa agcagcacaa atgtatttaa atggccaagt
     5641 agatcaattg tttttggtcg gaggggtggg gcatgcgact agaatccttt atgagaattt
     5701 tgaaaagcaa ggatttcatt ttgaagatgg catgagtgaa agtgaaatct gtcgtcagta
     5761 tctaaaagaa gtctatgatt tgcccgataa ggcttttctg atagaatcga agtctacaaa
     5821 ttctggagag aacgccactt tttcactgga gattcttcat tccttaggag ctgtaccaga
     5881 aaaaatctta ttgatgaatg atccgacact gcaacgcagg actcgagcaa cttttgagaa
     5941 agtatggcaa aatgaacaaa ctattttcgc gaatgctgtg ccatttgtgc cggaaatcct
     6001 tcacttttct gaagagatca tctttacagc aaaagaactg aatcatcaat ggcctaagga
     6061 atatttccat gcgctagtat taggagaaat ggaacgtctc catgacgatg agaacggcta
     6121 tggtccgaaa ggaaaagatt ttattcctca cattgatata tcagaggaag tttggtcttc
     6181 ttacaccaga attaaagagt caataaaaac ggatttttct cgtacttgac cattttagca
     6241 agcgaatcct cctttccaaa taaatttggt tgaaattttc aatcgtttat aagataatga
     6301 ggagcgtgtt gaatgtaata gctaaaagaa tggacgctag ataagagaag aggattcaaa
     6361 tggaaaatca aaataaattg tatcattttg ttggtatcaa aggttcagga atgagctcct
     6421 tggctcttgt tttacatgaa caaggtctaa atgtccaagg atcagacatt gaaaaatatt
     6481 tctttacaca aagagattta gaaaaagcaa atatcacgat tctgccattt aacgcagata
     6541 atgtaaaacc agggatgacg atcattgcag gaaatgcatt tcctgattca catgaagaaa
     6601 tccagcgagc aaaagaatta ggtttggaag tgattcgcta tcacgacttt attggtcatt
     6661 ttatccaaaa ttatacgagt atcgctgtaa caggctctca cggaaaaaca agtacgactg
     6721 gactgctctc tcatgtattg agtggtgtcc gtccaacaag ttatctaatt ggagatggaa
     6781 caggacatgg tgatccgcaa gcggaattct tttcatttga ggcctgtgaa tatcgccgtc
     6841 atttccttgc ctattcacca gactatgcaa tcatgaccaa tatcgatttt gatcatccgg
     6901 attattatac aagtatcgac gatgtattta cagctttcca aacaatggct ggacaagtga
     6961 aaaaagctat ttttgcgtat ggtgacgatg cttatttacg caaattaaaa gcaaatgtgc
     7021 cgatttatta ttatggtgtc acagaaaacg atgatatcca agcacgaaac atcgaacgca
     7081 caacttctgg ttctgctttt gatgtctatc atggggatga atttgttggg cacttcactg
     7141 taccagcatt cggtaaacac aatatcttaa atgctttagg tgttattgca gtagcttatt
     7201 tcgaaaaact ggatctgaaa gaagttgcag aagaaatgct gactttccca ggagtaaaac
     7261 gtcgattcag tgaaaagatc gtagcagaca tgaccgtagt cgatgattat gcgcatcacc
     7321 ctgcagagat caaagcaacg attgatggcg cacgccaaaa atatccagat aaagaaataa
     7381 ttgcagtttt ccaaccccat acgtttacgc gtacgattgc attgatggat gaatttgcag
     7441 aagcacttga tttagctgat aaagtttact tatgcgatat ttttggttcg gctcgggaag
     7501 aacaaggaaa cgttaagatt gaagaccttg gagcgaaaat caaaaaaggt ggagaagtca
     7561 tcaaagaaaa taatgtatct ccattgttag attatcatga tgcagtagta atcttcatgg
     7621 gtgctggaga tgttcaaaaa tttgaacaag cgtatgaaaa acttttaagc agcacgacga
     7681 aaaatgtact gtaatacaat tactgcagtg tccttataaa aatagaaaga gggggagtgg
     7741 caaaaatctt gccaagcccc cttttttgca acttatgata aacggtgttc aaaagcttgc
     7801 atctgcggta ataagcccaa tcaaatcaaa aacatgagaa gctgtttttt cttggctgtt
     7861 cttattattt gatgcaggac agctttttca cgacctctgt aaactatagg aatctgttaa
     7921 atcaagggag cagaacttgc cttttacgct acttttggta caataatgta tgattaaaaa
     7981 taaactggag ggtttctatc ttgaacatag gtaagccgag aaaactagga aaaacaataa
     8041 atgaaatcga ggaaggcgac tcgttatcct taacagaatc tattgaagat aaccagcttt
     8101 tgctgtattt gggattgacg aatgatgcaa atcctttgta tatccaacat gactttgcac
     8161 aaaaaacaga atatcaacag ccaatcgttc catcagtgat gctgatgggg atcatcacaa
     8221 gtgcaatttc aaagcatctt cctggaccag gatcacatgt tgtcaatttt tctgtcaatt
     8281 ttattgaacc ggttttccat tatgaaacac tgacttttca gtttgaggtc ataaaagtag
     8341 ataagatgaa ggatgtggtg acgatttctg tagaagcgga gaatacgaac gaagatcgtg
     8401 tgttggatgc tgtcgttatg gtccaaccgc cacaagttcc tgaattggaa atagcaatcg
     8461 aaagtgaagg tgaattgaat gaataatcca agtacatcaa tgtcaggtat caatcgtttt
     8521 tatgcaaaag tttatggatt tttaggtatg ggacttgccc ttagtgcagt cacttcttac
     8581 ttggctcttt gggtatttcc tttgcaatta gcaactttta tcaataattt tccactaggc
     8641 tttgtgggtc tgtggatcgt tgaactgatt ttagtaatgg ttctaggcgt aaaagcacaa
     8701 aagaatccga gcttagcaat cggtggattt attgcctact ctcttttgaa tggcataaca
     8761 ttagcggtta ctcttgcata ctacgatatt ggaacagtaa cgcaagcttt cttgacagct
     8821 actttgatgt tcatggggat gtctgttgtc ggagtaacaa caaaaagaga tcttagcggg
     8881 gttggaagag caggacaggc agctttaatc ggagtgatca ttgcactagt cttgaacatc
     8941 ttcattttga gaagtagcgt agttgagctg ttcatctccc tactgttagt cgtcatcttt
     9001 gcagggatta cagcttatga taaccaaagc atccgtaata tctatgtcca gacaaacgga
     9061 acagcacata gtggaattgc tgtattcatg gcattgaagc tgtatttgga ctttatcaac
     9121 ttgttcttgg cgtttctgag aatctttgga cggaacaact aaaaaagaaa aaaattcatc
     9181 agttggggct ataccgaatt atttggtatg gtcccgcttt ttcttttcgc attccgcagg
     9241 attctttgtt agaatgaata agaactttac agtaaggagt taagaagatg acaaaaaata
     9301 aattattact cgttgatggg aacagtgtgg cttttcgcgc attctttgcg cttcacaatt
     9361 ctcttgaacg tttcaaaaat agaaacggtc ttcatacaaa tgcaatctat gcattcaata
     9421 cgatgtttga aaatgttatg caaaaagagc aaccgactca tgttctggtt gcgttcgatg
     9481 caggaaaaac gacttttcgg acagaaatgt atgcggagta caaaggtgga agatccaaaa
     9541 cacctggcga attcaaagaa caaatgccat atatacgtga tttgttgaca ggactagggg
     9601 tacagtatta cgaattgccc aattatgaag cagatgatat tatcggtact ttggcagaaa
     9661 aagtagacaa agatcaattt gatgtcgtcg tgctttcagg tgatcgggat ttgactcaat
     9721 tagctagtaa agaagtaaaa gtggatatca ctgtaaaagg tgtcagcgat atcgaaagct
     9781 atacaccaga acatgtagcg gaaaaatatg atgggttgac gccaaaacag attattgaca
     9841 tgaagggact agccggagat gcctctgaca atatccccgg tgtgactaaa atcggtgaaa
     9901 aaacagctat caagctattg aaacaatatg gctcagtcga aggaatttat gaacatatcg
     9961 atgaaatgaa gcaaagcaaa atgaaagaaa atctgataaa tgacaaagag caggctttct
    10021 tatcgaaaaa attggcgacc atcaatactt cagcgcctgt ggatgtctct atcgactcat
    10081 tgaagtatga agggaaaaat ctcgataaac tcgttccgtt ttataaagaa atggatttca
    10141 atcagttcct ttctaaattg aatatcgtag aagaagaagt aaaaatggat gatattttgt
    10201 ttgaagtcgt tcatgaattc aaagaggaaa tgtttactac ggatatggcg ttgtacgtgg
    10261 aaatgatggg cgacaattat catacagagg aaatcgtcgg agtagcgtgg gggacagaaa
    10321 agaaaattta tgtcacgaac gatctatctg tattcgaaaa ccgagcgttc cattcttgga
    10381 tcactgatcc gactcggctg aaaaaagtat atgatgcgaa acgaacttat gttgctttaa
    10441 accgctatac tggaaaaacg aaaggaatcg attttgatgt cttgctaggt gcataccttc
    10501 tcgatacgaa tgaaaaaagc acagatatcg aaggaatagc tgctcattac ggatacaacg
    10561 atatccaatc agatgaagct gtatacggca aaggggcgaa aaaaggttta ccagaagaag
    10621 aggaactctt ttttgctcat ttagcgcgaa aagtagctgc aatcaatgcg ttgacaccaa
    10681 aattgagcca agaactggca gaaaaaaacc aagaagatct tttccggaaa atggaactgc
    10741 cattgtctat cattttggca gagatggaaa taagcgggat caaagtagat gcgactcgct
    10801 tacaagaaat gaagggcgag ttctctgctc gcttaagaga gatcgaacaa aaaatctacg
    10861 aagaagccgg cgaagaattc aatttgaatt caccaaagca attaggagtg atcttatttg
    10921 aaaaaatggg attacccgtt atcaaaaaaa caaaaactgg atattccaca gccgtagatg
    10981 tactggaaca gcttcgtgaa caagcaccaa tcgtagagga tatcctcacc tatcgtcaaa
    11041 ttgcaaaaat tcaatctacc tatgtcgagg gactgctgaa agtgatccaa tcagatggaa
    11101 aagtgcatac ccgttatgtc cagacattga cccaaacagg acgattaagc tctgttgatc
    11161 ctaatttgca gaacatcccg attcgattag atgaagggag aaagatacgt caggcatttg
    11221 ttcctagaga aaaagactgg ctgatttatt cttctgacta ttcacaaatc gagttacgcg
    11281 tacttgcgca tatctcaaat gatgaacatt tgaaagctgc cttcctagaa ggtcaagata
    11341 tccatgccag cacagcgatg cgtgtatttg gaatcgaaaa ggcagaggat gtcacaccaa
    11401 atatgcgtag gcaggcaaaa gcagtgaatt ttggtatcgt gtatggcatc tctgattacg
    11461 gactttcaca aaatctagga atcagccgaa aagcagcaca gcaatacatc gatacgtact
    11521 ttgaaaaata cccaggtgtc aaagaatata tggaaagaat cgtacgcgaa gcaaaagacc
    11581 aaggctacgt cgaaacgctg tatcaccgcc gtcgatatct gccagacatc aattcaagaa
    11641 attacaatat ccgttctttt gcggaacgaa cagctatcaa tacaccaatc caaggaagtg
    11701 ctgctgatat tttgaaaatc gcgatgatcg aattggataa acgattaaaa gaaacaggtc
    11761 ttcaagctac gatgctgtta caagttcatg atgaacttgt cttcgaggta ccggaaaaag
    11821 aacttgaatc attagataaa ttggtgaagg aagttatgga acaagctgtt tctcttcatg
    11881 taccactgat cactgacagc agttggggaa aaacgtggta cgaagcaaaa taaacattgt
    11941 ttaagacaga gcaatataat gaaggtgaaa agaaaaaatg ccagaattac cagaagtaga
    12001 aaccgttcgt aaaggattgg aacggttagt agtaggaaaa acgattcaaa aagttcaggt
    12061 cttgtggccc aagatcatcg agcaaccaga gacacccatc tttgaggctt ctcttgttgg
    12121 cgaaaccatc caatccattg gacgtagagg gaagttttta atttttcatc tcgatcattg
    12181 tgaactgatc tcacacttgc gaatggaagg aaaatatcaa tttacgaaag aaaatacacc
    12241 aatcgataaa catacgcatg tacttttctt ttttgaggat ggaagccaac ttcgctacaa
    12301 cgatgtacga aaatttggtc gtatgacaat cgtcgaaaaa ggagcaagtg caacttaccg
    12361 aggaatcatg aaattgggac ctgagccttt gccagatagc ttcttattag cagatttcgc
    12421 aaatggactg aaaaagtccc acaaagcaat caagccattg ctgctagacc aacgtctagt
    12481 gactggtcta ggtaatattt atgtagatga agcattatgg gaagcgaaga tccatccaga
    12541 acaaccggcg aacacactgc gccccaaaga ggtagaacaa ttacgtctat cgatcattga
    12601 tgttttggat cgtgcaatag aagccggagg taccacgatt cgcagttatt tgaatgcatt
    12661 aggagaatca ggcggatttc aagtagcact gcatgtctat cagcaaactg gcaaaccatg
    12721 tatacgttgt ggaacgccga tcgtgaaaac aaaagtggca caaaggggga cgcactattg
    12781 tccgaattgt caaaggctaa aaatcgcaaa gtaggatttg ttttaggttt gacgggtgga
    12841 atagctaccg gtaaaagtac ggcagccaaa gtctttcagt ctcatggatt tcctttgatc
    12901 gatggcgatg tgattgccag agaaactgtt gagcctggaa cgcttgctct gaggaaaatt
    12961 gtatcagtgt ttggccaaga ggtattgcag cctgatgggc aactagatcg agggaaactt
    13021 gggatgattg tttttccatc aaaggaaaaa cggcaaaaac ttgatcagct attagatccg
    13081 tttatccgaa aagcgataaa agatcaaatc gcctctctaa gttccaatca tcctttagta
    13141 attgtagata tcccattgct ctacgaaggt cattatgacc actatatgga tgcagtagct
    13201 gtcgtttata ctacgccaga aactcagctt cagcgtttga tgaaacgtaa tcaactgacg
    13261 ctagaacaag cgcagcagag aattagtagt caacttccaa tcgaagaaaa aaagcagcgt
    13321 gcggatattt tgttcgacaa caatggaaca aaagaaaaac ttgtgggaca aatcgaaaat
    13381 tggctttttg ctaatcgctt tctttgagta tctgtgtcaa ttttgctatc ctgagtatat
    13441 agaaagtgag ggatagcgat gggatcatta aaaaatcaaa aggataaagt cgtaggtaac
    13501 gcaaaagaaa aaatcggcga gatgctcaat aaagaaaatc taatcgatga aggtcgtacg
    13561 caatcagaaa cagcagaaga aaaagaaaag cagtaccaag caagaatcga tgaaatagaa
    13621 gaccagcagg cagaagaaga agcgaagcgg caagatttga ctcaagaacg aaaaaatccg
    13681 acacctgatc gtagtaaaac accgctccca gaaagtgacc acattcccga tgagcaaaaa
    13741 atcgaggaac agcgaatgaa acactataca ggaatcgaag agaagttata aaatagtaaa
    13801 aaatcgagat gtgacttgaa gcagatgaga aggtgttcat ctgcttcaag ttttttgtgc
    13861 tagatgacta aatcagcttt tgtgtaagtt atttctcagt aatcaaggtg atcagtattg
    13921 gaaaattgaa atttagtaag gacaggggac acaattacgc atgaaagtag aaaaaattgt
    13981 tcttatatcc gtgttttttg ggtaggacag tcgcgtttgt gatttgtttc gtgttataat
    14041 acaactagtt gtaaagaaag acacatcctt tatataaaat gcgatgcaag aggtgagaca
    14101 aaatgagatg tcccagatgt caacataata attcacgagt aatcgacagc cgtcaagcag
    14161 atgatggacg tgcgatccgt cgccgtcgcg aatgtgagaa ttgttcgttc cgattcacta
    14221 catttgaacg aattgaagct gctccgttat tagtaatcaa gaaaaacggg gaacgcgaag
    14281 agttcaatcg cgataaaatt ttaagaggac tcatccgttc agcagaaaaa agacctgtag
    14341 ctatggaaca aatggaacaa atcgtcaacc atgtcgaaaa ccgcgtacgc agtctcggag
    14401 aaaacgaagt atcgacgaat ctaattggtg aatatgtgat ggaagattta gttgatttag
    14461 acgaaatcgc ttatattcgt tttgcaagtg tttatcgcca gttcaaagat atgtctgttt
    14521 tcttaaaaga gttgcaagat atcgtggata aagcaaaaga aaataataaa gagaataata
    14581 aagaaaacaa ttaagaaaca aaaaggaggc atttccttgg aaaatgcttg gaaagagctt
    14641 caaccaaaaa atatttacca aggaagaaaa gcggctccgc tgagagaaga cagcaagaac
    14701 gccttgctgt atctttatca gccactaatc ggcggagaag ccttgagtat ctatttcacc
    14761 ctgttatcag aaatctctca agaatctggc aaaggtcctg aagggatgca tgcagatctg
    14821 cttagtagtc taggctgcgg tatcccgcaa ttttatgatg caagaaaaaa gctagaagga
    14881 atcggcttac tggatgttta ttttaaagaa gatagtgatc tgggacctcg ttttatttat
    14941 gagctgttag aaccactcat tcctaaagca tttttcaaag actctgttct ggcttttctt
    15001 cttctggaaa aagttggtga acgacgattc cagaagctta cccaattctt tgaacctaaa
    15061 aggcatacag cagagggata tcaaaaaatc acaaaaaaat ttttagatgt ttatcgcttc
    15121 aatgaagcag tgtacgctgc taatcaagaa aacatcgaat cgctgaataa atcttttgaa
    15181 gaatctgaat cagtagcatt acttgaagca gattcgacat tcgattggca gtttttgaat
    15241 gattggctgg caaaaaaaca tattgcaagt cttgatgatc caatcatcaa acaagtcgac
    15301 atgtatcatc agctttacgg tttcgatgaa ttgacacttg gcgaactgat cagtcaatct
    15361 tatgacttta gtgaacaaaa agtatctgaa aaagaactgc aaaaagccat tttggcaatg
    15421 cagagacgtc cggtgaagca aacaaaagaa acagttgcgg tttctgaaaa cagtaatgaa
    15481 ccctcgtcaa acgaacattt gacaaacgca gcgcttcagc tgataaaaga agcacagagc
    15541 attccaccga tgaaatatct ggaagcaatc aagtcagaaa aaggcggata tgtttctaaa
    15601 caagaaaact ggttactgca agatcttgtt tctcaatcag gtctttccag tagcgtgatc
    15661 aatgttttgc tgaattatgt actagtgatc aagaataatg ctagcttaaa tgccagttat
    15721 gtaaatacag tggcaaatga atgggcacaa aagaaaatcg ccacagcaga agaagcgatc
    15781 cagcacattc ggcaaataag taatcaagca aaacaatcaa aacaaaagaa gcaaacaaat
    15841 tatcaaactg gcaagcgaaa tgttcgacgg gaaaagcttc ctgattgggt caaccagcca
    15901 aaagatgaaa aacagatcag cagcgagaaa aaagcagaga ttgaccgccg ttttaaagaa
    15961 tacctggcaa aaaaggaggg tgatatctag tggaagacgt aggcaaagaa ttgaagagga
    16021 tcatcagcaa acgcaattat caagaaaagt atgctgaaat gatcgctgaa gtcttaaatg
    16081 atgcggatgt tcaagctttt ctaagcgcac accaagaaga attgacacaa gcggatattg
    16141 aaaaaagtta cgctaaattg tatgaattcg tgcaagaaaa gcgtaaatat caaaaaaacg
    16201 atccaacgat gatcgctccc ggctatgagc cgaagctgac gttgaatttt cattatgtgg
    16261 atgtcactta tgtaccaacg gaagcattgt tagcacggca aagacaagaa gaaatcagaa
    16321 gccgagtaaa agcaatggat atgccaaaag atattcaatc ggcttctttt gaaaactttg
    16381 aacgtacaga tggacgagga tatgcgagct tagaagccct agattttgta gagaaatata
    16441 ctgctgatcc aaaagggtat cataaaggac tgtatctagt aggaagtttt ggtattggaa
    16501 aaacatattt attaggagca acagcgagag aattagcgat tgctggattc accacgacgc
    16561 tagtccattt tccgacattt gccgttgaga tgaaacaggc aatcggaaaa gatcaagtgg
    16621 cagaaaagtt agatgcagtg aaacgttcac ctattttgat gatcgatgac attggtgcag
    16681 atgcgatgag tagttggata cgagacgaag tattcggtgt tatccttcaa tatcggatgc
    16741 aggaacagtt accaaccttc ttctcatcga attttacaat ggatgaattg gaacagcatt
    16801 tgtccgtcac tcaacgtgga gatgaagagc cgctaaaagc gaaacggata atggaacgca
    16861 tacgttattt gacaaaagaa atcgaaatga ctggtcgcaa ccgacgcaat ggttgaacag
    16921 ttgacaagtc caactgacat ttgacctagt agtaaaagca ggtcagcgaa tcattatatg
    16981 gacagaaatg accggagaca atcaccgcaa caagtaatgc agaaacttaa acggaggatt
    17041 ttttatgagg gctatttttt ctagtatatc tgtagttggt ctattttttc ttttagcagg
    17101 ctgcggtagc gcagatagca acacaggttc tgttgattcg attgatgcgg gtcaaacagt
    17161 aaaaacaagc agtacgaaaa cggagagaca aagcatattt gtcggaacga ttgaaagtat
    17221 cactcaagta gaagcagaag aagaaacgct tcagttgaaa ctcgtcgatg tcatggccaa
    17281 agaagatcca gcgaatatgg gaacgtcatt cagcaatgac ggagtgatct taaatgctac
    17341 tgctgaaaaa ataagcgtgg acataaatga attgaaaatc ggtgacaaag tccaatttac
    17401 attagttgag atgccgatca tgacgatgtc tatcccaccg caagttcctg gagattcgat
    17461 tattgatgtt tcattgaaca aataaccaat aacatttttt caaaaataag aaaatgttgt
    17521 tttatagaac gaaaaggtat attttttaag gagatatggg aattttttta ttcctagtct
    17581 ccttatttta tttggaaaat gctataatta tctagaagga gtgaagctat gaaccattta
    17641 gatttatctc agcgcttagc cgcagtgggt gactttgtcc ctgaatccgc tcgtttagca
    17701 gacattggat cagatcacgc ttacttaccc gttgcattga tattaagagg aaaaatcgaa
    17761 tatgcagttg ctggtgaagt cgttcaaggt ccttatgaat cagcacgtaa gcaagtcaaa
    17821 aaagatggat tagaagaccg tatcaccgta cgccttgcaa atggtctaga tgctatccag
    17881 ccacaagatg gtattacagc tgtcacgatt gcgggtatgg gaggttcact gatccgagat
    17941 atcctagaag ctggcaagtt gaatcaacgc ttaagcggac aagaacgttt gatccttcag
    18001 ccaaacatcg gagagcgtac attacgatct tggctgcaag aaaatcagta taaaatccaa
    18061 tcagaaaaaa tacttgaaga aaataaaaaa atatatgaaa tcattgtagc cgaaaaagaa
    18121 caaaaaccag ttacttacaa tgaaatggag ctgatgttcg ggccatttct gcttgaagag
    18181 aaaagtgaga tcttcaaatc aaaatggaca agagagcttc aacaaagaaa agctatccgt
    18241 gaacagctca aacaagcagc taatcaagag gcacgtcaaa aacaaatcga gcaggaaatc
    18301 caaatgattg aggaggtttt aaagccatga caatcagcgg acgcactttc atccaaaagt
    18361 ttgaaagcta ttgtcctcag tggctagctg aagaaggaga cccagtcgga ctgcatatcg
    18421 gaacattgga taagccaatc caacgagtaa tgatgacatt agacgttcgt ccagaagtgg
    18481 tcgaagaagc aatcaagaaa aaaatcgatt tgctgattgc taaacatcct ccaatctttc
    18541 gtcctgtaaa acggctggtt actgatcagc cacaagaaaa aatgtatgca gatttgttga
    18601 aacatgatat tgccgtctat gcagcacaca cgaacatgga tattatttgg gatggactga
    18661 atgactggtt ttgtgaactt ctcggtattg aagtggagag ttatttagta aagactcatg
    18721 agattcatta taaaaaactg gccgtttatg tacctgtgga tcatgcacag aaaatgcgtg
    18781 aagtattagc tgcagctggt gctggtacac aaggtgatta taccggtaca agttttacct
    18841 cgattggaca cggtcgattt actccggaag ctggtgcaca accagcgatt gggaaagtag
    18901 ggaagacaga acaagttcaa gaggcaaaag ttgaggttat tttgccggaa actatcgaaa
    18961 aacaagtgat tcaagccatg cggtcagctc atccttatga ggaacctgct tatgatcttt
    19021 tcgctataga tgaaccagtc gaaatgtttg ggttaggacg agtaggtgaa ttgccgcaag
    19081 aaatttctat agaggcattt gtagaacaag taaaagaagc gttccaacta gatggactga
    19141 gaatagtcca gccaaaaaat gcaaaatcct ctgtgaagcg cattgcgatt tgcggtggaa
    19201 gcggtgaaaa attttatccg caagcaatcg cccaaagagc agatgtctat atcacgggag
    19261 atatctatta tcatacggct catgatatgc aaagcgctgg gttgattgca attgatcccg
    19321 gtcattatat tgaatcgtta tgcaagcaac gatttatcga gaaatttgaa tcttggaaac
    19381 aagaagaaaa ttgggatatt aactttttcg tttctgaaac aaatacaaac ccatttcaat
    19441 ttaattaaaa ggagagaata ctatgtacga aaatcttttg ccacgttttt tacgatatgt
    19501 caaaacagaa acacgctccg atgcaacaag caaaacgact ccatcgactc agacacaagt
    19561 tgcttttgcg caagaattga aaaaagaatt agaagagtta ggcttagctg atgttttcta
    19621 taatgaagat aacggattcg ttatgggaac attaccaagc aatgttgatc atgaagtgcg
    19681 ttccattgga tttatttccc acatggatac agcagatttt aatgcagtag gagtcaatcc
    19741 acaaatcatt gaaaactatg atggcgagtc tacgatcgta ctagatgcgg aagaacgcta
    19801 tacattgaat acaaaagatt ttccaaattt gaaaaattat gaaggccaca cattgatcac
    19861 aacagatggc agtacattac tgggagcaga tgacaaatca gggattgcgg aaatcatgac
    19921 tgcaatggag atcttgatca ataatccttc tatccctcac ggtaccatca aagtggcttt
    19981 tggtcctgat gaagaaatcg gtgtcggagc ggataaattc gatgttgagc aattcgatgt
    20041 agattttgct tatacagtcg atggtggtcc tttaggtgag ttgcagtatg aaacgtttag
    20101 tgctgctcaa gcaaatatta cgatccaagg gaaaaatgta catccaggga cggcaaaaga
    20161 tacaatgatc aatgctctac agttagcaat cgattttcat aatcagcttc cagccgatga
    20221 agttccagaa aaaacggaag gatacgaagg tttcttccat ttgatggcat tggatgggaa
    20281 tcctgaagaa gcttctatga gttatatcat tcgcgatcat gatcgtgaaa aattcgaagc
    20341 acgcaaagca caaatcacac atatccaaga agagttgaat cagcgatttg atcaagaacg
    20401 aatcaaagta gatatgtatg atcaatatta caacatgaaa gaaatcattg aaaaagatat
    20461 gagcatcgtg gaattagcga aacaagcaat gatcgagctt ggtatctctc cagtgatcga
    20521 accagttcgt ggaggaacag atggttcaaa aatctcttac atgggtattc cgacgccaaa
    20581 tctatttgcc ggaggagaaa atatgcacgg acgattcgaa tttgtttctt tacaagtaat
    20641 ggaaaaagca acagacgtga ttgtaaaaat tgcagaattg aacacaaaga gttaaaatcg
    20701 aaaaagaaca gcttatgaca aggcgttcgt cccaatgatg gaatgtcatc tgtctaaggc
    20761 taaagggcag gacaatggaa ttggttgtcc cgccctcttt tttgaagagt aaataggagg
    20821 taaagaatgg aaaagctgct agttttttat aacaaaagtt cagggaaaga tgagggtgag
    20881 aaattagcac gctggtttca agattatgta aaagaaaagc agccagaatt atatgtagaa
    20941 ctagctgaaa ctggcccatc gattgatgat gcggaattag tcaagaaagc agaagccata
    21001 gaagctgata ctttagtaat tataggtgga gacggcacga ttcatcatat cgtccaagcg
    21061 ttccaaaaaa cattatctca ataccatgtc ggattacttc caggtggaac tgtcaacaac
    21121 cttgcacgtg ttcttgagat tcccctcaaa aaagaagaag ctgctgacgt gatcctggaa
    21181 ggcaatgtac ggaaaataga ctatggtcaa gtcaatgatg aagtcattat ctctacactg
    21241 acaatcggaa tcttagcaga tacagcagtt tggatcagtc aagaagaaaa acagcgttac
    21301 ggctcgtgga tctttatcaa gaagtttttt aagttattgc tgaagaagaa aaaatatcat
    21361 ttggatttag aaacagacga ggagcattgg aaaggaaaat cccaactgtt gacagtcacg
    21421 atgtctaact ccgtgggcgg ttttacaaat tttgacgact cagcgaccct agatgatgga
    21481 aaatttcatg taacgatcgt accttcctta gatattttcc gatttatttt ttatttgcct
    21541 agaatcatga gaggcaaatt ctatacgatc cccggcatta cttatttcac agccaaacgc
    21601 atgaaaatcc aagcgcagga aaaatctgtg ggcactcgaa cggatggaga cacgacagat
    21661 gatcttccag ttgaattatc tgttgtatca aaaggattaa cagtttattc accaaagaaa
    21721 gaagaaaaag cttagaaata gtagttaaca gaaagttgga agaagagtaa tggatgtatt
    21781 gttacacaat cctttgtggt tttacctttt cattgtgaat ttttacttat tttgtctgat
    21841 ggggtatgat aaacatcagg ctagaactag cggctggcgt gttcctgaga ataatttgat
    21901 atttgtcagc ctgttaggcg gtggtttggg aggattgatc tctcgaaaag ttttccatca
    21961 taaaactcgg aaaaagaagt ttactatagg attcgctgca ggtgtacttg ttgatttgat
    22021 tctcatttat tttttccatt aaggagcagg agatgtcgtc taaaggaacg aaaactaaaa
    22081 tcattctgaa tctgcttttg ctgattgtga tttttgccat tatttattat ttaatcaatc
    22141 aatcatttgc agatatcttt gccgaattgc tgtctaccag tgttgttgtt ttggcagcgg
    22201 ctgttatatt agggacagtt taccaaatcg ctgaagggcg ctcaatcaaa gaaattgctg
    22261 aacctttcgc gccgaatttt acaacgaaag atgggttctt gacctctagc tatattgcgt
    22321 tttaccgaat cgtttcgttt ggtacaggaa cgctgatctc ggagatttat ttttataaca
    22381 aaaaaggaat gaagctatcc cagggagcag gggtgacagc actccatatg attatgtaca
    22441 aactggcagt gatcacttat gcattagctg gcttggtcat ccaattctct cttttttatt
    22501 ctaatggtcc atcaatgatc tggtttatca tcgctggtat ccttttgacg atcgtgattg
    22561 ttgccttttt attggcgatg tccgtcagct tgaatgttca aatatttttt gtcagcttat
    22621 cgaataagtt atttaaaagt gagcgtttgc ggaaatgggt ggatacatgc aatcagcaga
    22681 tctactcttt aagggaaacg gtccaaacca ttatccaaga caggactgcg ctattgcgga
    22741 tttatctatg gaatatgtta aaacttgtat tttggtatgt acttccttat ttgattctga
    22801 ttgaaaacca tcctaaaata gattttctac tgaccatttc gtttatcagc tttgcagtag
    22861 tgctttcagg ggttattcct acgcctgctg gtattggttc ctttgaattt gtctatttgt
    22921 tattattcag accagttgtt ggaacagttg acgcagtttc ttcgctttta ctgtatcgtt
    22981 tcagttcctt tatcttgcca tttctctatg gatttgtgta tgtgattgct gaacggcgaa
    23041 gagtgatcaa agaagaaata atagaagtaa aaaaagagaa taaagaaaat caagggtagg
    23101 tcttttataa gatctaccct ttttatcaaa tcaaagaaaa ac
//
LOCUS       DACEUZ010000033        21663 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.033, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000033 DACEUZ010000000
VERSION     DACEUZ010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21663
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.033"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(13..996)
                     /locus_tag="IXG44_001106"
     CDS             complement(13..996)
                     /locus_tag="IXG44_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HAQ1657838.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(1001..1648)
                     /locus_tag="IXG44_001107"
     CDS             complement(1001..1648)
                     /locus_tag="IXG44_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ1657839.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(1733..2365)
                     /locus_tag="IXG44_001108"
     CDS             complement(1733..2365)
                     /locus_tag="IXG44_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ1657840.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(2381..2932)
                     /gene="coaC"
                     /locus_tag="IXG44_001109"
     CDS             complement(2381..2932)
                     /gene="coaC"
                     /locus_tag="IXG44_001109"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HAQ1657841.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(2929..3666)
                     /locus_tag="IXG44_001110"
     CDS             complement(2929..3666)
                     /locus_tag="IXG44_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HAQ1657842.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(3663..4652)
                     /locus_tag="IXG44_001111"
     CDS             complement(3663..4652)
                     /locus_tag="IXG44_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAQ1657843.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            4910..5452
                     /locus_tag="IXG44_001112"
     CDS             4910..5452
                     /locus_tag="IXG44_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1657844.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            5469..6554
                     /locus_tag="IXG44_001113"
     CDS             5469..6554
                     /locus_tag="IXG44_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1657845.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            6566..7372
                     /locus_tag="IXG44_001114"
     CDS             6566..7372
                     /locus_tag="IXG44_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1657846.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            7373..8200
                     /locus_tag="IXG44_001115"
     CDS             7373..8200
                     /locus_tag="IXG44_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1657847.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            8197..9270
                     /locus_tag="IXG44_001116"
     CDS             8197..9270
                     /locus_tag="IXG44_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1657848.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            9698..12394
                     /locus_tag="IXG44_001117"
     CDS             9698..12394
                     /locus_tag="IXG44_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1657849.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            12471..12959
                     /locus_tag="IXG44_001118"
     CDS             12471..12959
                     /locus_tag="IXG44_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ1657850.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            12971..13465
                     /locus_tag="IXG44_001119"
     CDS             12971..13465
                     /locus_tag="IXG44_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1657851.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            13486..14295
                     /locus_tag="IXG44_001120"
     CDS             13486..14295
                     /locus_tag="IXG44_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1657852.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            14292..15113
                     /locus_tag="IXG44_001121"
     CDS             14292..15113
                     /locus_tag="IXG44_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ1657853.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            15164..17263
                     /locus_tag="IXG44_001122"
     CDS             15164..17263
                     /locus_tag="IXG44_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HAQ1657854.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            17260..18378
                     /locus_tag="IXG44_001123"
     CDS             17260..18378
                     /locus_tag="IXG44_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HAQ1657855.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            18417..19295
                     /locus_tag="IXG44_001124"
     CDS             18417..19295
                     /locus_tag="IXG44_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ1657856.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            19441..19638
                     /locus_tag="IXG44_001125"
     CDS             19441..19638
                     /locus_tag="IXG44_001125"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1657857.1"
                     /translation="MLLRFLFSFCCALYTLRIFTGGVHLNSNILCYFMSVVIVIVAYQ
                     CMFIFRNVDFIILGLMFEFFW"
     gene            complement(20662..21474)
                     /locus_tag="IXG44_001126"
     CDS             complement(20662..21474)
                     /locus_tag="IXG44_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1657858.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
BASE COUNT         6620 a         3740 c         4596 g         6707 t
ORIGIN      
        1 atcagcatga ttttattcag ttatcttcaa aatgattttt ccgatgtttt tgtttgcttc
       61 catatattca tgcgcttgtt tgactgcgct gaattggaat gtacggtcaa tgattggccg
      121 aatcttgcca ttttctagaa aaggcccagc tttttgcata aaagtttgtg cgagctctgc
      181 tttatactga tcacttctag gtgtcaataa tgtaccaagc agttggacac gtttcccaat
      241 caatacacca aggtctatct caggcacgat ggtcccacct agcataccaa tcaagaccca
      301 acgtccatca acggctatag aagctaaatt cttttcccag taagaagcgc cgatgaaatc
      361 caaaataaca tcgactcccc gaccttcagt gatttttcgt acctcttctt gaaagtcttg
      421 ttttttatag ttgatcaatt catctgcacc aagttcttta caaaaggcga gtttttcttc
      481 tgatcctgca gtaacgatga ttttcgcgtg agaaagctgt ctagctaatt ggatcgctgc
      541 tgttcccaca ccacttgctc ctgcatggat caagacagtt tcgccttctt gtaattttcc
      601 aagccagtaa agtgtttgat aagcagtcaa aaaaacttct gagattccag cagcagaaac
      661 atagtctagc gtatcaggga gaaggattgc acgatcggca ggcatcacag catattcggc
      721 ataaccacct agattgacta atccgtatac ttttgtccca ggagtaaaag tggagatatc
      781 acttttattc tcaactacct ctccagctac ttcgactccc aaaatagggt agggaggttt
      841 caaagagact gattctcttt tcataatatc tgttcgattg acagctgctg tatggacttt
      901 gatcagtaat tcgccttgtt ttggtttagg aacaggcact tcaatttgtt ctaatacatc
      961 tacagaacca ggatgcttga ttgaaatggc acgcatggat ctattcctcc tttgcccatt
     1021 cttggaatgc tgtattcaac tctttgctac cttcagaaag tactagagtc gtacgagaaa
     1081 tatccgtttc ttccactaga gcggcaagta cagcagcaac tgtagagcga gtgatcgttt
     1141 ttgatgcctt ctcattaggc ttagttaatt gaatggaagt aacttcttca tcgttagtca
     1201 atgtgactgg ttgaagaatg acaaaatcta agttagttcg tttcagccat tcatctgcat
     1261 aatgtttagt aatataataa tctgtcatgg actcaggcca ttttgctggt tcatctgcat
     1321 aaacagcact gaccatcaga tagcgagaga catgcgcatt ttcagctgcg atcactgttt
     1381 tgattgcgcc atccaagtct acttgcagaa gattctttcc ttgagagccg gcagcaaaaa
     1441 tcaatacatc gatgcctttg aatgcttcgg tcattttttc cacggaccaa gttaaatcaa
     1501 aagagacata cgaaacattc tcttttttta ctaatgtttg ggaagcaaca tctctcaccc
     1561 ctgcaaaaat ttcatgggaa cttggagcta aatcttttat caggtgtcgg ccaatttgtc
     1621 cgtttgcacc tacaacaaaa attttcattt ggatcatcct ttcgacgtca aatctttcca
     1681 ttcaatagta acaccaaaag agaaaaaaat cagagatcgg ctacgatgac tatcagttag
     1741 taagagaagg gtgcttgaag tatcttaaca agacattgca tactgctgca gtcgcaagac
     1801 ctgcgacgat tccttcaggt ataccacttg taaaaataac cgctagaata gctgaataaa
     1861 cagcatttaa atttgttccg atgactttgg cataatcttg ctggaataaa aagtagatca
     1921 tattcatcac taaaatcgta ttggtcaatg aaccggaaag tccagctaag aataaagaaa
     1981 cggaatctgc tttttgtttc attagtttgc gcagaccttt ataaacgaaa taaggaacca
     2041 ccccaatcaa gatgcgagga ataaacgcga tcagcaaggc tttccagctt ccatgttctg
     2101 ttccgatgac tgggataaac ggagaaaaga caaaagacaa aggtgtttgg ataactgtgc
     2161 tacggatcat gctgatcagt ccaaatgtac cgccaagaaa agcacctagt cttggtccta
     2221 aaacgatcga agcaatgatt acaggaatat gcatggttgt tgcattgatt ggaccgatag
     2281 ggataaaacc taaaggcgta acagctaata aaatcaaaat agctaaaaac atcgcagtta
     2341 atgtaaagtt ttttgtattt ttcataagta ctcctttttt ctaagcattt ctttcattaa
     2401 gagtcagcat gacctgttcg atgatcgtct gaatatcagc taaggcacct tttccaaaat
     2461 ctccacaagc taaaagagat tctcttggtt caatttcctg atagccgaca gatctcaatg
     2521 tttccagatt acgctgagtg atcgggtttt gatacatatt cgtgttcatg gcaggggcaa
     2581 tcaattttgg tgttcccact ggaagcgcca ttgcgactgt tgaaagaaga tcgtctgcaa
     2641 tgccgtttgc tagcttgccg ataatgtttg ctgtagcagg cgcgatgaga aacaagtcag
     2701 cttgttttgc tagttcgata tgattgatta cctgcggatt tgattcttgc atgacgtctg
     2761 tatggactgc gcgcttcgac agagattgca aggttaatgg cgtgataaaa tgtgtgctgc
     2821 ttttagacat aatcacatct acctgggcac ctaatttgac gaattgactt gtaagatcag
     2881 cgcttttgta agcagaaata cttccagtaa cgcctagtaa gatctttttc attttgattc
     2941 ctccagtcta attttctcag taatcaatgc agcaatctca gatttacttt gagcttcttc
     3001 cactgttcca tctttagata agagatagcc gtggtgtgtc tcgccatgga tttccgtcaa
     3061 atcatttgcg aaaataaaat cagttttgtt atttatcaga ctattttttg cgactgccag
     3121 aagagattcc tttggtacat ctactaatag tttgaaacca atgatttttg cttttggctg
     3181 ccattcacgt agataagaaa tgatttttgg tgttttcttc aaaacgagca caagatgctc
     3241 ggtgttagaa gagatctttg aaacagtggt atctgtctgt tcattttccg caaaccaatt
     3301 gtccagttct tcttgcgttg aagaagtggg taggtttttt gccagctgtt cttctgaaaa
     3361 actaaaagca ggagtaaaat cactgactgc catactgtgg ataatcgcat cgtagtgttc
     3421 ttttttcgtt agttcttcta attgtaaaaa taagtccagt gcagattcga tcgtataaat
     3481 cgttatattg gagtgtcttt tcggtttcag tgcttgtttc gtagtcacgt aatcgattgt
     3541 ggtatccgga ttggcagcta aataatcagc taatgcttgc cctaatcgtc ctgtcgaatg
     3601 atttgtaata gcacgtactt gatcgatttt ttcagtcgtt cctccagctg tgattagaat
     3661 cttcatttat ggtcacctac atttactagt gttcttcctt ggtgtgttcc agcaagtagt
     3721 ttagatgctg tttcaggcaa ttgatccaga gtaatctctt ggacgaccag ttcatctgca
     3781 atctgcaaat ccgccatctg ttgccataaa gaaagacgtt gatctattgg tacattgaca
     3841 gaatcaattc caatcaactg gatgtttcgc agaataaaag gcaagacagt cgcgtttagt
     3901 ttgattcccc cagcattgcc acataaagct actgcaccgt tataagagat caatggaaga
     3961 agactgctta gctgctcgcc acctactgtg tcgatgacat agtcgatttg ctgttttcct
     4021 aaagcttttg ttgtctctgg cagaaattca tcaggtcgta cgacttgcaa agcacctaga
     4081 ctttttagcc atgtgacaga ctcttctttt ctggaaaagg caatgattga ctggaatcct
     4141 agtttatgta acagggctat cgctgtactg cctacaccgc cagaagcacc agtaacgaca
     4201 acttttttat cggaggtcat accttgacgg attaaagcgt tcacacacaa catagcagta
     4261 aaccctgctg taccaaggat catcgcttga cgcagcgtca tattttttgg tagaggaact
     4321 aaccagtctc ctggtacttt ttgatattgg ctataaccgc ctggatgggt gacgccaagc
     4381 ccaaaaccag tagcaagtac aagatcttct tttgaaaaac gtggattgtt actttggacg
     4441 acagttccgg ataaatcaat ccctggagtc atcggatatg ttcgtatcac ctgaccagac
     4501 tcgctcattg ccaatgcatc tttataattg acatcagaat aggcaatttt taccaagact
     4561 tcattttcag ccaactcagt aaatggcaga tcactgattg ctgtatggaa aacaggttcc
     4621 tttattacct gaaaagatcg aaaagcgttc attcgtgaga cactcctttt aaaattttct
     4681 tttatcatac cataaagcct actcttttat tcttgttctt ccgtaaattt agacaaaaat
     4741 taggtgaaaa ataaagaatc aaacaactag aatttattcc aaaagataaa gaaatattca
     4801 gcaaaaaaaa ttttacaaag ggttgccttt ttcaataaat ttcgtatact aatacgagtg
     4861 gtagtaaaca ggaagtttac tattagaaaa cttcgattcg gaggaaatta tggaaattgg
     4921 tgagaagtta agaaatttgc gcatccaaaa gaatttgaca caagaagaac taggcgaacg
     4981 cacagatttg tcaaaagggt atatttccca gttggaacga gatttaagtt caccatcaat
     5041 ggaaacattc ttttctattc ttgaagtctt aggtgtgact ccagaagaat tttttcatca
     5101 agaagcagcg aatctgcaag tggtttattc aaaagaagat catacggttt atttcgatga
     5161 agaaaacggg tatgaattgg aatggctcgt ggctgattcg aatgaaaaag aaatggagcc
     5221 tgtcttgctg acttttgaag gtgcaggaga atataagacg tttgaacctt cattatctga
     5281 aacgtttatt tttgtattag aaggggaatt agagttaagc ttaggagaga cggtctatgt
     5341 ggcaaaagca ggacagtcga tctattatca agcgacaaaa caacatcaat taaggaatca
     5401 tctcaaaaaa cgaacaaaag tattgattgt tgcaacgcaa tcatatttgt aatatagggg
     5461 gtaaaaacgt ggggaaacca atcatttcat ttgatcaagt agtgaaacgc tatgatgatg
     5521 aaacagtatt aaaaaaagta agttttgaga tcgaacaagg aaaattttat acactgcttg
     5581 gtccttccgg ctgtggtaaa acaacgatcc tacgtattat agctggattc accgaagcta
     5641 cagaagggga catctatttt gaagggaaac ggttgaatga tttgccagct aataagcgac
     5701 aagtaaatac agtcttccaa gattatgcac tttttcctca tatgaatgtt tttgataacg
     5761 ttgcttttgg attgaagatc aagaaaatgt ccaaatcaga tatcgaaaag aaggtaaaag
     5821 atgctttgcg tatggtccag cttcctggat acgaaaaacg ggagattagt gaaatgtctg
     5881 gtggtcaaag acagcgtgta gcgattgcac gagcaattgt aaatgaaccg aaagtattgc
     5941 tattagatga accgttatcg gcgcttgatt tgaaactacg tacagatatg cagtatgaac
     6001 tgcgggatct gcaacaacga ttaggaatca cgtttatttt tgttactcat gaccaagaag
     6061 aagcgttggc aatgagtgat gagatttttg tcatgaataa agggaaaatc gtccaaagtg
     6121 gtacacccgt agatatttat gatgagccaa tcaatcattt cgttgctgat tttgttgggg
     6181 agagcaatat cgtggacggt gtaatgatcg aagataatct agttgagttt gttggtaagc
     6241 aatttgaatg tgtcgatggc gggatgcgtc taaatgaacc agttgaagtt gttttacgcc
     6301 cagaggattt gacgatcacg acaccagata aaggaaaact agtcgtaacg gtcgatactc
     6361 agctgtttcg tggtgtccat tatgaaatca tctgttatga tgaacagcaa aatgaatgga
     6421 tggtccattc aacgaaaaaa gctaaagaag gaagcaaagt aggattggcc tttgagccag
     6481 aagatatcca tgtcatgcgt ttcaacgagt ctgaagaaga attcgatgca cgtcttgaca
     6541 gctacgaaga atagggggga caagaatgaa aacaatgcgc aggatctatt ccgtccctta
     6601 tattttgtgg ctcggtttat tcgtgatcgc gcctgtatta atgatcattt atcaatcttt
     6661 ttttgatatg aatgggcaat tcacgctgaa taattatcta tcttatttta cgtcggggac
     6721 atcgctttct atgacgatca attctgtatg gtatgcattt ttgatcacgt ttttcacgct
     6781 gatcatcagt tacccgactg cttatttttt aacaaaactg aagcacaagc aattatggct
     6841 gatgctgatt attttgccta catgggtgaa tctgctgctt aaagcttacg catttattgg
     6901 gattttcagt gtgcacggca gcgtgaatca tttctttgat tttatcggga tcgggccgaa
     6961 acagatatta ttcacagact tcagttttct gttcgtggca acgtatatcg agattccatt
     7021 tatgattatg ccaattttca atgcattgga agagatgaat ccttctttga tcagtgctag
     7081 tagagattta ggcgcgaata gctttgagac cttccgacga gtgatttttc cgttatcatt
     7141 aaatggtgtg aaaagtggtg tccaagcagt atttattcct tctctttctt tattcatgct
     7201 gactcgtctg attggcggaa atcgggtgat cactttagga acggctatcg aacagcattt
     7261 cttggttaca caaaactggg ggatgggttc tacaatcggt gtgattttaa tcattgctat
     7321 gttcatcgtg atgctcctga caggcgagaa gaaaaaagga ggtcgctcat aaatgaaaaa
     7381 atttaaatgg tcgtatcttt atttagggat cgtcttttta ttcttatatg caccgatctt
     7441 ctatctgatt ttttattcct tcaatgaagg aggaacgatg aacagcttta caggatttac
     7501 ttgggaaaat tatgaagctg tttttgaaga tacacgtttg atcattatcg tattgaatac
     7561 gttcatgctg gctttcttat ctgcattgat cgccacgatc atcgggacat ttggggctat
     7621 gggaatctat tacacgaaaa aacgacgtgc acggacagcg ttgttgagca tgaataacat
     7681 cttattagtc tctcctgatg ttattatcgg tgctagtttc ttgattttct ttacactact
     7741 aggattcgga ttaggttttc agagtgtatt gctttcccat attgctttca gcattccaat
     7801 cgtcgtcttg atggtcttgc caaaattgca ggaaatgaac gattcaatgg tcgatgcggc
     7861 tagagacctt ggtgctaata atttccaagt agtcaaaaat atcattttgc cttatctttc
     7921 tccaggaatc atagctggat attttatggc atttacctat tctcttgatg attttgcggt
     7981 tacatttttc gtcacaggaa atggctttac gacgctctca gtagagatct attctcgtgc
     8041 aagacaagga atcagtttag aaatcaatgc gctaagtgcg ttagttttct tgttctccat
     8101 gatattggtc attggatact actttattag taaagaaaac cgtccaaaac ggctgaagaa
     8161 aaacagaaga gtacgcgagg gggtggcacg gctgcgatga aaaaattaca atctctgttt
     8221 ttaggtattc tcatcattat tgcggtgctg ctgcttggtg ttcgtcaact tgaaaaaaca
     8281 agtggcatga gtggagcaaa ggtacttacc atctataact ggggagatta tatcgatcct
     8341 agtttgatca cgaaatttga aaaagaatat ggatacaaag tgaattatga aacatttgat
     8401 tctaatgaag cgatgttcac aaaaatccag caaggtggga caaactacga catcgctata
     8461 ccaagtgaat atatgatcca aaaaatgatc aaagaaaaac tagtacttcc attagatcat
     8521 tcgaagatca aaggattaaa taatattgat ccacgatttt tagacttgga ttttgatcca
     8581 gataatcagt attcgattcc atatttttgg ggaactttag ggatcgtata taacgataaa
     8641 ttctttgatg caaatcaaat taagcattgg gatgatttgt ggaagccgga attaaaagac
     8701 agtttgatgc tgattgacgg tgcacgagaa gttatggggc tttctttgaa tagtttaggc
     8761 tattctttga atagtaagaa tatggcacag ctgacagaag cagcaaataa attgaatcat
     8821 ttaacaccaa atgtcaaagc aatcgtggca gatgagatca aaatgtatat gatcaatgaa
     8881 gaagcctccg tggcagtgac attttctggc gaagctgccg atatgatgtg ggaaaatgaa
     8941 catttacatt atgtgatccc accagaagga tccaatcttt ggtttgataa tatcgttatg
     9001 ccaaaaaccg tgaaaaataa agaaggtgcc tatgatttca tcaactttat gctagaaccg
     9061 gaaaatgcgg cacaaaatgc agagtatatt ggttattcta caccaaatga agaagcaaaa
     9121 gcgttattgc caaaagacat ttcgtcggac gaacaattct atccatctga tgacacgatc
     9181 tcccatcttg aagtctacaa agatctagga tcgaaatacc taggcatcta taatgaccta
     9241 ttcttagaat tcaagatgta taggagataa tggcaaaact cagtaagaat ggggacttat
     9301 caacgttcta aagagctaaa aatcgtcctt tttctatctg agttttccaa tcttctacca
     9361 aaagcggatg aaacgccgct ttttttgtgt tattttattt tttctaccac gtttttctac
     9421 cagagaaaag tggacaaaac cattgtaaaa tagggcccga gacgggctag taaatctaag
     9481 attgaaaaag ttacagaagg ataaagtaat tggaaactat gcaaatatac ttaacccttc
     9541 tgtaatctat taataaaact aatatatgaa tccagttcaa tatttgtagc tatgaaaaaa
     9601 cataaaaatc atattagata aaaactaaat tggtagataa attattttgt cagatatctg
     9661 aggcaaatat tcgactattt gtgtttcaaa gtaaaaattg gaatacaaat atagaataga
     9721 catattgcag ccgatagttt attatgcttt tagacacatt aaactggagg aaaaaatgaa
     9781 aaagagcgct aaagatcgag tttttgatga gctgatacga ctaacaggga atatgtcttc
     9841 tgtcactgcc gagaaaattt cgaagaatct aggtatctct cgtcagaatg ccagccatta
     9901 tttgacgaga ttagtggaag ataaaaaggt cgagaaatta ccgggaaagc ctgttttgtg
     9961 gaagccttta gatgaatatg cggttgtaga taatacggag caaataaacg aagcctttca
    10021 ttctgtagtt ggtcatgacg gaagtctgag agaggtcatt caaaaatgta tagccgctgt
    10081 caaatatccg cctaacggac tcagtgtatt aataaatgga gccacgggtg ttggaaaaag
    10141 ttttttagcc acaaaaattt ttgaatacgc tatccatgag caaattattg aaaaagatgc
    10201 accatttgct atcttaaact gtgccgatta tgcggacaat cccgaactgc tttcggcaac
    10261 actgtttgga tacaagaagg gggcctttac gggggctgaa aaggataccg atggtttatt
    10321 ggcaacggcc aacaacggct atctttttct cgatgaggtc catcgcctat cgaaagaaaa
    10381 tcaagaaaag ctctttctat ttattgatac tggaaactat cgtccagtcg gtgaaaatgt
    10441 aaattggcat tctgctaagg ttcgttttat tttcgccacg acggaaaaag gtgaaaatta
    10501 tttattagac acctttgatc ggcgtattca aattagtgtt atgttgccaa cttttgaaga
    10561 tcgccccatt cgagaacgat tagagctgat tcaattattt tttcaaaatg aagcggatgt
    10621 tttacaaaaa gatattattg tttcaagaga agccttgtct ttaatgttat ctcatccctt
    10681 tagtgggaat attggtaagc tgaagaatat cattaaaatc agttgtgcgg atgtgtatag
    10741 tcgaacacaa gaagaaccat taacgattgg gaaaaatgag atcttgattc agttaaatat
    10801 gctggaatcc gaagtagaaa gcttgccgat tgctagttta ttgataaaga aaaatcaaaa
    10861 gataaaaacg gatcgaatca aaccgtttga ttatgattcg attttggaaa aaattactca
    10921 gctgataaat gaaggtaatt tcaatgaaaa tttttcttcg ataaagaatg aagtacagca
    10981 tttggtgcat cacattgata aggattcttt tttatcaatg agtagaaaat tgatgattca
    11041 gatgtttaga aaggtctggg tatccgtttt aggaaaaacg tacggattga cgacgagtgg
    11101 acctgttgca gaaattatta gcagatccta tgtcatttcc ccaagctata agacagactt
    11161 aaccgggata aatcgtacat tagctgcaaa tctacctcgg acgacttact tatgtaatca
    11221 gttcttggag catttaccac cgctctcaga aaaggacgag aactttctaa gactgcttat
    11281 tgtcgtgact ttatctgatt atgtagatga atccatcgag ctgaagggcc tgttgcttgc
    11341 tcacggagat agtacggcta gcagcattca agcagtagtg aatcaattat gcggcaatta
    11401 tgtttttgaa gcgttggata tgccagtaga gagtaatgtg tcagagattg tcgaatcgac
    11461 aaggaatttc gtgaaacggc aagtgaatac agatagtcta gtgctgatcg tagatatggg
    11521 ctctttaaat caaatttaca cacagatcaa gaacgattta aaaggggagt tacttcttct
    11581 gaataatttg acgacttcca ttgcacttga tattggcttg aagatgacga tgaatgcgcc
    11641 atttaaacag attgcggagg atgcagcaga caagtattca atcaatgttc aatactttga
    11701 aggcttttct caaagtagca acattatcat ttcctgtatg tcgggtttgg ggatttcgga
    11761 caaattgaaa gatgtttttc gtcatcattt atcggatgaa tccatcgaag ttttcacgaa
    11821 ggactacaga gaactacgtg aattaatcga ccaaaatgat gaaacttatt ttgacaagac
    11881 aaaattggtc atcacgactt cggatcttcc acgttccttt tcaattccta atattaatgt
    11941 ttatgacatc ttagacggtg atggagcagc caatttggag catattttga gtcctgaaat
    12001 gaagccggca gaatttaacc gcttgattca ggagctagta aaattctttt cgttggaagg
    12061 aattgcggac cgtttgaact ttctgaaccc aacgatcgtg atgagtgaag tggaaacagt
    12121 catttctaaa tatgaaaatt actatcattt cactttaaat ggccgtgtaa aactaaatct
    12181 ctatatgcat acggcattga tgatggaacg tttattcttg gctcggacag aaaaatctga
    12241 agaaccgaat tatgatttga aaccagatga agatgaattt tattatgttt ctagaagtat
    12301 ttttcaatca atggagatga aatataattt taaagtcaat gcttatgagc tttcgctgct
    12361 ctatgaatta ttaggaccgt atattcaaaa ataaagtgtc taaaaaagtg tctaatctgt
    12421 tttttttagg ttagacactt tttttaatgt gaattaacgt gattaaacca atgtttttaa
    12481 aagttggcac aggaattgct tatataaaag tgaacagaaa gaaaggaatt aaccatatga
    12541 agaaaattct aatagcgact cacggtcact tagcttcagg ctttcttagc agtattcaat
    12601 tactgacggg gaaggttcaa gagattacgg tgattaatgc ttatgtggat gagtgtgatt
    12661 ttgaacagga gttgaatgta tttgtaaacc aggtgaacga gcaggatcag atttttgttt
    12721 ttaccgattt atttggcgga agtgtcaatc agaagattac caaaaccttt ttggagaaag
    12781 agattgctgt aactattttg tctggcttta atctgccaat tctgctcgaa atagtattgg
    12841 caaccaagga attaacaaag gaggatattc aggaactggt aaataaatgc caacaggaat
    12901 taaagatgac cattcttgat gaggtacatg tagtaaatga agaggaagat tttttttagg
    12961 aggaagaaaa atgattaaag cagtgcgaat tgatgaacga ttaattcatg gacaagtagc
    13021 gatgacttgg acaaagagtc taaatttaac cgggttagta gtagcaagcg atgaggcggc
    13081 taccaacgat attcaaaaga tgacattgaa gatggctgca ccatcgaatg tcaaagtcat
    13141 tattaaaaca gttgatgacg cgattcattt gttaaaagat ccgcgggcga aggacatgcg
    13201 cttgatggtt ttagtcccta atgtcaaaga tgcggtaaaa gttgcgaaag ctttcccaac
    13261 ggaaatcgaa ttaatgaacg tggggaatgc tgggaaaatg acaccgccat cggaagataa
    13321 gaaaacctta tcaaaggaag tgatgttaac gacgtcagaa attcaagact taaaagaatt
    13381 agttgaactt tatccagata ccgtttttca gcctacaccg gcgatggaaa aacgttcagc
    13441 tatctctatt ttgagtgatt tttagaaaaa ggaaggagaa aaattatgta tgatgcattt
    13501 gtagtcgctt tggccgtttt tattggagtt gctggacatg aaatgttcgg gatgtccatg
    13561 ctgagtcgtc cgattgttgt tgctccgtta gcaggagcgt tgatggggga tttgcagacg
    13621 gggttgattg tcggggcgtc actagaagcc atctttatgg gtgtcgtcaa tatcggaatc
    13681 tctagtacgg cggagccttc tttggctgct gggctggcta cagcgttcgc aatcaaaatg
    13741 ggaggaaacg tggatgcgat tattcccatt gtctttccac tagcagtatt aggattacag
    13801 ttaatgaata tgattttttc ttttgtatgt ggaccgatgg ctgctaaatt tttcaaattt
    13861 gcaaaatctg gtgatcaaaa aggaatcatc aatctgcact tctttatgtg gtttgtccac
    13921 tatgcgcttt atgctcttat tccattcttt gccgttttat ttggagcaga tgttgtcaaa
    13981 agcgtcttgg ataatattcc agaggtcatc atgaacggat taacagtcgc tggaaatctg
    14041 ctgccggcag ttgggatggc gatgctgtta cggatgcttt ggagtaaaaa tattgctgtg
    14101 tggtttttct ttggggccgt tgttatggcg tatctcgagt taccattgat tgcaattgcc
    14161 gcagttggaa cgattattgc aattgttata gcccaaagag atctacaact aaaaaatata
    14221 tctgttcatg cagttcaaag cgatgctgct gtaaatgcaa cgcttgacca agaagaggag
    14281 gatttctttc aatgaaatta gctgatggaa tttctaaaga tgagaaaaaa ttggttcgga
    14341 agatgttttg gcgctcgatg actatgtatg cttcggtgaa tccggtgacg atgggcggcg
    14401 gtggtttcgc ctattctatg atgcccttca ttaatcattt ctacaaaagt gaagaagatc
    14461 gagctgcggc gctggaacgt catacacagt atttttcgac gaccattcct tgcgcgtcct
    14521 ttgtcatggg gattgcggca tcgatggaga aggaaaattc tgaaaaagct ggttttgacg
    14581 tggaaagtat caacgcgatc aaaacgagtt tgatgggacc attggctgga attggcgatt
    14641 cattgttctg gggagtttgg cgtgtcgtta ccgcgggact agcgatcaac ttagccaatg
    14701 ccggaaatat tttggcacca attatctttt tagttatgtt caatatacct aattacttga
    14761 tgcgatacta tggcggcgtt ttaggctatt cattaggctc taaatatatt gaaaagctgt
    14821 atgcgaacgg gttaattgaa attctgacaa aagctgctag tatcttgggt ctgatcatgg
    14881 taggtgcaat gacgatcagt atggtcaaat tcaataccgt tttgagcttt tcaatgaagg
    14941 gcgaagaact aatgaatgtc caagaaatcc tcgatagtat ttttaaagga attgtaccgt
    15001 tattggtaac attgagctgt ttcaaattac tagataaaaa agtcggcgtc atcaaaatta
    15061 tttttggaat ggtcgtcttg ggaattttat tgtcattact tggaatcgca tagtaggaag
    15121 ctgcagctac taggctgcag tttttttagc aaggagggaa aagatgacta ttaatgaagt
    15181 aatttattca cccaaacacc cattttttaa tgctgagtta aacgacggaa atgtgactac
    15241 ttcaaacgaa tacagctact atcggaatat tcctgatgta aagggattga ccttcgatgt
    15301 aagtaatcaa aagacattag caaaccttga tgtttgcggg acgattcaaa cgttgacctt
    15361 ttatcgagaa aatttactaa ctgaggaaaa accaggtgtt tgggtcaata aacagctctc
    15421 tcaaaccaat tcgttatcct taaaggttga agtaaacggg attgtctacc atttgtcaga
    15481 atcggatcat cgtattgaag tcgatgtgat tcaagactgt ttgcctagat atattcatca
    15541 ttattcgacg tttaaggtca ttgtagtatc ctttgctccc atactggcta ataagcgttt
    15601 aagtatgctt gttcaacagg tttacattgt gaatgaaaca cacgaaagcc ttcaagtttc
    15661 agtgaaagat gtgccttcgt atcaagagaa gtattcggat cagcaaaatg tgttgatttc
    15721 tcaaaaagga aataaggaca cgatcgcaca acaggaagtg gcaagcttcg ccattgcttt
    15781 tgtggaccca aatgcttttg aagaagtaat gacttttcag gaatctgata tcgaaaaatg
    15841 gctgaaggaa acattgaact attttgctca gctatttggg cagctgtcaa tgccaaacga
    15901 tagagttgtc catctctatc gtcgtgcact ttatcaaagc ttttctagtt tcggtatgaa
    15961 tcgacaggga caggtcgtcg gcagcaattg gggcagctat cctgctacta atcgaatttg
    16021 gaataaggat atgtattacg catcattgcc attcaccatg tttgaaccgg agttatgtca
    16081 aaaaacggtt ctatggtttg atcaatatgg cgttaagttt cctggttcaa aatttacagg
    16141 aggtatcaat cattctctga gtaattcgtt atccagtgcg ttactggcta gtctttattt
    16201 tgaacatact ggggatacaa aattctttga gcggtgtccg caggttctac tgaatgcttc
    16261 aagaattatt gaggacatat tagctcaacg tcggtcaggg gaaccaatgc tttttccatc
    16321 tgtttggtta tcagacgcct ttgcacttgg aaagtatcat accggcagca atctttgtct
    16381 ttggaaggcc tgtattggac ttgcagaaat ttttgaagcc ctagaacaac tgagtcttag
    16441 caagcgatac aaaaacattg cctgtaaact aaaggaatca atttgtaaac aaatgactat
    16501 cgatggtcct tttggtgagc aatttttgga gggaatcggt gatgaggaga aaacaaccta
    16561 ttcagtgaag cactatcaaa aaccgattct tgagcaaggg ttaatctttt taagtgatgt
    16621 gattgtggat ggaaaaatta atttattgat gcatgatgga gaagaatcag acactacgtt
    16681 gatgcctttt tatcaatttt tagataataa agatcattta tatcagaaca ccatggcctt
    16741 ttctgctagt tctttcaatc caacctatag cgaggaaatc aaaggcatca cttggggatt
    16801 agaatccggc gccacttttc ctggctttat cactgtttta atgagcgagc tgcacaaccc
    16861 tcaagctttc gtaacccgat tagaagaact aatttgtttg gcagatttag acggttcgtg
    16921 gtggtggtgg ccgtatcgct tagaggctca acaaggagaa gtcgtgcgag actttggctg
    16981 cggcaaatgc ggctgggcat cgggtctttt tgtcagccta atgatcagtc aatatttcgg
    17041 attgaaattc aaaaaaggag tattgcaaat tgatcctgtt gataatttaa cgttttcttg
    17101 gaagaatttg aagttcggtc aagccttcat ttcgattgaa tgtacgcaat ccgagttgag
    17161 tatttcaaat ttgtcagaaa agccattgaa aataagtgat acgaaaaaaa ttcttcacgt
    17221 agaaaaggga aaaacgattc acattcaaag gagaaaaaga tgagtatact aaaaaatatt
    17281 acccaattta aaaaaatcca gagtcgagca atcactcccg aaaatccaac aggcgagaaa
    17341 ggtaaagctg cgatgttgcc aagcgagcta ggaccaagcc gcaaaggtcg tggcgcaatt
    17401 ccgctgactt ctggtgaaga aactgtgatt gcggatatta ctggaacggg aataattcgt
    17461 catatgtgga tgacgattcg tgatcgaaca gaatttggca gttttgtttt acgtgattta
    17521 atcttaagaa tttattggga tgggtcggat agccctgctg ttgaaagtcc tttaggggat
    17581 ttcttttgta atgggtttgg agagcgttgc gaggtgaact cgttgccgat agtggttaat
    17641 cctacagggg gctttaattc ctatttcgaa atgcctttcc aaacatccgc taaaattacg
    17701 atcgagaatt tacatccgaa ggatatgtcc tcattttttt atacgataaa ttacgctttg
    17761 gtggacgaat tggaagaaaa tagcctatat tttcacgcaa attggcatcg ggaacgcatt
    17821 accaaattac aggaggatta taagttaatt gatcgattaa agggtcaagg ctattacgtg
    17881 ggtacttacc ttgcgcttac ctgcctagaa cgttattggt ggggcgaagg cgagtttaaa
    17941 ttctacattg atggggatag tgagtatccg accgtaacct caacaggttc agaagattat
    18001 tttggcgggg cgtgggcctt tcatgagtac gatgagttcg gacgtccaca tgcgaaaaac
    18061 tattcaacag cctttttagg gtatccttac caatcaaata gagatgccac aagagatcgt
    18121 tttgaaacgg gaaaattgaa tgccgttcat gcctttggtg acgacgcaat gccacgacat
    18181 ggtctttatc gttggcatct tcaagacccc atctgtttta ccgaagattt atcggtcact
    18241 ttacaacaaa tcggcaacga tgatgttcgc ttatttgagc ggcaagacga tgttgctagt
    18301 gtcgcttact ggtattcaac agaagcttca ggaaatacta aacgtcattt cactgatcga
    18361 gaacgcttgc cgcgttaatg aagtgttttt cacatattcg aagggaggaa cctatgatga
    18421 tatatggggc aattgaagct ggagggacta aatttgtctg tgcagtcggt actgaggaat
    18481 taacgatttt ggaaagagtc agttttccca caacaactcc agttgaaaca atgaagcagg
    18541 tcattgattt ttttaaacct tttaaggaac aattggcagg catcggggtc ggctgctttg
    18601 gtccaattga tattcaatgc aaatcaacga gtttcggtca tatcacatca acaccaaaac
    18661 ttgcttggca aaatttcaat tttgtaggaa cgctaaagca atatttcaat attccgattg
    18721 aatggacaac agatgtcaat gcggcttgct atggtgaata tgttgctggt tctggcaaag
    18781 ggttagcagg tgttgcatac ttcactgtgg gaacaggtat cggaggtggt gccttgttca
    18841 atgggacttt cgtggaaggg ttcagtcatc cggaaatggg gcatatgttg atcaagcgac
    18901 atccaaagga caatttttca ggaaactgtc ccttccacca agattgttta gaggggatgg
    18961 cagcaggacc agcgattgaa aaacggttgg gtgtaaaagg ccagaattta ttggctgacg
    19021 attcgttttg gcagattgaa gccttttacc ttgcccaatg tgcatacaat acaacactga
    19081 tgttttcgcc agatagaatt atttttggcg gtggtgtaat gaaacaagaa cacatgaaga
    19141 aaaaagttca ggataaattt gttgagctta taaatggata tgttgaaata ccaccaattg
    19201 atagctacat tattacgccc gaattaggtg ataatgccgg aataatcggc ggattagcat
    19261 tggccagaaa agcggtaaga aataagcaac cataattaat gaatgtttca ttgatactca
    19321 tgtcaaaagt atttaaccga gtaatcaaat gatcaaatga agttggataa agcgaaaatg
    19381 aattcaaata aaaaggctct aagtcaaatc gtgttgtttt cattaaatca ctgaaaggaa
    19441 atgcttctgc gtttcctttt ttctttttgt tgtgcgctgt ataccttgag gatattcact
    19501 ggtggcgtac acttaaatag taatatattg tgttacttta tgtctgtggt aatagttata
    19561 gttgcttacc aatgtatgtt catttttcga aatgttgact tcataatcct tggcttaatg
    19621 tttgaatttt tttggtgata gattttttca gccgtataaa ataaatcgta attctattat
    19681 agagagttgt gattataaaa agatagcaag aaaaaaaaag aaagtttcta tacattttaa
    19741 atattttatt cctatttcta ttgctaaaaa agtagaaatt ctatatttac ctttaactct
    19801 cgcttctctt gcaaatgtag ttagcatttt aatcgataat aaactcaggt agttatatta
    19861 ccttgaaatg attgataatt ttgtcacttc tatttttttt gcaataaatt ttttatttta
    19921 gtgaaaaata agaaactatt tctttgaagg cgataagtta gaaaaatatt attgtagtct
    19981 tttctttatt aggaatcatc actgttgtat cttattacct tttctgtttt tcttattaga
    20041 gaaaaataat ttgagaggta gatagttttt attggaggaa gtaattatgt atatttttga
    20101 aacaaaaatt tttcattgca ataattgcaa caaagaaatt tcatcggggg aagaggtttg
    20161 gacaaaaatg gaaatttccg ccaaaagtaa ctgctcctca actgaaatca attaaagaac
    20221 tagaatttga gaatgcactg gtaatttgta tgacatgtac tgatgaagta tttcgaaaac
    20281 atttttaaag gttgagatgt atatagattt aaccaatcat ttatctctct cggaaaagga
    20341 atattaacta accagcgggt tatcaaaaat aggtaggaaa actgtactta atggcttatt
    20401 tcgcgctgat tatgagaata tcagtctgtt tgattaattt ttagggttca aaatacagag
    20461 acaaactcca ggtcgggttt cgccacaagc gaattacccg atctggagtt ttgtctattg
    20521 caggaaggcg tagccggact gcaggaataa agcccccggt tctaacaggg ggctaacaaa
    20581 gaacaggaaa ctgtgacaac tacccattgg ggagtgtcct agcagtattt tctttgtcac
    20641 gtgcttatca attttgtcac ctcattttga tgatggatac gctgcattta acaggcgttc
    20701 tttctcgact tccatcacga atggtagata aagatattgc tgttctagta gatattgatt
    20761 gtcctctgtg tactgataag aagcggtttc ttctgaatag accaacgggg caattcgcac
    20821 atcgctggtc aattgattaa ttgagtatag aaggatggct ttctcaccta gttccttggg
    20881 taaatgtttc ccaaaatagg aataggattt ttgatttgct aaatagacga gtatgcgatc
    20941 cacactaaag ttggagaagg taaagggcat catgtgcaga atatgatttt gattatcatg
    21001 ttgcaaatag agctgggctt ccttaccaaa attagttgaa ccaagataaa gctgcttttt
    21061 ttcatcgata cattggaaag taaactggtc ttgttcagtt ggtggaatga gatttgtaat
    21121 aaaaaattca atcattcgat ccatttcttt ttcactaaca aggtcagcgt gtttgatttc
    21181 gtctactatg agaccttcaa tcttaggatc aagcttacct gccttttttt ggagcatttc
    21241 taaaatgtat tgattctctt gatcaccata caaaagatat tcattggtga catgacctat
    21301 ctctgcgatt tggtgaaggg tttcggtttt tggaaggttg atcccacgtt cccagttatt
    21361 gactgtactt cgcggtaaat caccaattag tttgccaaat ttctccatac tcaactttaa
    21421 ttcttggcga atctgccgaa ttcgattgcc gactgcttgc ttgtctatct ccatgaaaac
    21481 tcctcctcag gataagttct taaatttagt atacgtgtgt acttaaaaaa agtcaaaaca
    21541 tattgactta aaataaatac aggcgtacaa taaaaatgta cttaaaataa gttcaatagg
    21601 ttctttgaca agtaaatagc agaaggaggt ttgtcgatga accaagttaa tcaacatgat
    21661 ctc
//
LOCUS       DACEUZ010000034        21514 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.034, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000034 DACEUZ010000000
VERSION     DACEUZ010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.034"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(270..1766)
                     /gene="lysS"
                     /locus_tag="IXG44_001127"
     CDS             complement(270..1766)
                     /gene="lysS"
                     /locus_tag="IXG44_001127"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HAQ1657859.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1877..2881)
                     /gene="dusB"
                     /locus_tag="IXG44_001128"
     CDS             complement(1877..2881)
                     /gene="dusB"
                     /locus_tag="IXG44_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HAQ1657860.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2882..3769)
                     /gene="hslO"
                     /locus_tag="IXG44_001129"
     CDS             complement(2882..3769)
                     /gene="hslO"
                     /locus_tag="IXG44_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HAQ1657861.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3986..6097)
                     /gene="hflB"
                     /locus_tag="IXG44_001130"
     CDS             complement(3986..6097)
                     /gene="hflB"
                     /locus_tag="IXG44_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HAQ1657862.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(6187..6732)
                     /gene="hpt"
                     /locus_tag="IXG44_001131"
     CDS             complement(6187..6732)
                     /gene="hpt"
                     /locus_tag="IXG44_001131"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HAQ1657863.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6732..8177)
                     /gene="tilS"
                     /locus_tag="IXG44_001132"
     CDS             complement(6732..8177)
                     /gene="tilS"
                     /locus_tag="IXG44_001132"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HAQ1657864.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8297..8767)
                     /locus_tag="IXG44_001133"
     CDS             complement(8297..8767)
                     /locus_tag="IXG44_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HAQ1657865.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8813..9238)
                     /locus_tag="IXG44_001134"
     CDS             complement(8813..9238)
                     /locus_tag="IXG44_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HAQ1657866.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9305..9574)
                     /locus_tag="IXG44_001135"
     CDS             complement(9305..9574)
                     /locus_tag="IXG44_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HAQ1657867.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9585..11180)
                     /locus_tag="IXG44_001136"
     CDS             complement(9585..11180)
                     /locus_tag="IXG44_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ1657868.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(11195..14716)
                     /gene="mfd"
                     /locus_tag="IXG44_001137"
     CDS             complement(11195..14716)
                     /gene="mfd"
                     /locus_tag="IXG44_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HAQ1657869.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14733..15293)
                     /locus_tag="IXG44_001138"
     CDS             complement(14733..15293)
                     /locus_tag="IXG44_001138"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HAQ1657870.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15646..16620
                     /locus_tag="IXG44_001139"
     CDS             15646..16620
                     /locus_tag="IXG44_001139"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAQ1657871.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16790..18190
                     /locus_tag="IXG44_001140"
     CDS             16790..18190
                     /locus_tag="IXG44_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HAQ1657872.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18268..18990)
                     /locus_tag="IXG44_001141"
     CDS             complement(18268..18990)
                     /locus_tag="IXG44_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ1657873.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(19069..19575)
                     /locus_tag="IXG44_001142"
     CDS             complement(19069..19575)
                     /locus_tag="IXG44_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAQ1657874.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSIDHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19698..21497
                     /locus_tag="IXG44_001143"
     CDS             19698..21497
                     /locus_tag="IXG44_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HAQ1657875.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WEEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
BASE COUNT         5972 a         4703 c         3864 g         6975 t
ORIGIN      
        1 ttttgttatg tttttgaata aatattttct tctgtctttt ggacaagtaa taatatacca
       61 actaaattta gcttggtcaa cgaatttttt gtattttttc aagttttttt caataaacga
      121 ttattcataa cttttctttc tttatcgttc gttttcgtct tctttaatgt ctagaatagt
      181 cggcattagc ttttgaactt tcgttattat ttattccttt ctacacagca aaagctgcct
      241 aaggaattcc ttagacagct tttattcgat tatctcattg ttgggaatag caaaacatcc
      301 cgaattgatt gtgcatcagt tagcaacatt actaatctgt cgatcccgat gcctagacca
      361 ccagttggcg gcataccgta ttctaaggct tccaagaagt cttcgtctac accgtgagct
      421 tcgtcatttc ctagttcacg ttctttttct tgcgcttcga atctttctct ttgatcaatt
      481 ggatcgttca gttctgtaaa ggcattagca aattcgcgcc ccactatgaa tagttcgaat
      541 cgatctgtga atcgaggatc ttctggattt ttcttcgcta aaggcgaaac ttcgactgga
      601 tgtccgtata caaacgtcgg ttgtgtcaat gtttcttcaa caaatgtttc aaagaattca
      661 ttgataacat gtccgacacc catagcttcg gttacttcta cattgtgttt ttcggctaaa
      721 gctaatgctt cttccaatgt catttcttgc cagaagtcta caccagtttg ttctttgatt
      781 gcatctacca tatggatgcg tttaaagtca ctttctaagt cgacagcttg tccatcatat
      841 gtgatttttg ctgttcctag tactttttca gctgcgttac ggataatacc ttctgtcaga
      901 ttcattacat cattgaaatc agtatatgct gtgtatgctt ctaacatcgt aaattctgga
      961 ttatgtgtag tatcaattcc ttcattacgg aaaacgcggc cgatttcata aactttttcc
     1021 attccgccaa caattagtcg ttttaaatga agttctagcg caatacgcaa atataaatca
     1081 atgtccagtg cattatgatg cgtgataaat gggcgtgcgg cagctccacc tgcctcattg
     1141 tgcaatactg gtgtttctac ttcaatatag ccattgccat ctaaatagcg acggatttcg
     1201 ctgataattt ggcttcgttt cataaatcgg tcaaagcttt cgcgattact gattaaatcc
     1261 aagtagcgtt gtctgtagcg ttgttcaata ttcgttaagc catggtattt atctggcagt
     1321 ggacgaagtg cttttgacaa aatcgtgatt tctgaagctt tgatagaaac ttcgcctgtg
     1381 tctgttttca tgatttgtcc agttacacca aagaaatctc caaggtctgc atgtttgaat
     1441 acttcatagg cctcatcacc tacttggtct tttcttacat aaatttgaat ttggccttca
     1501 cgatcttgta gatgcgcaaa tccagctttc ccttttcctc ggcgagtcat catacgacca
     1561 gctacactag cggtcaatcc catttctgct agttcttctt ttgttcttgg atcaaatagt
     1621 tcatgcaatt cttctgagtt gtgcgtgcgt tcaaatcgtt ttccaaatgg atcgattcca
     1681 tcttcacgca attgttccat tttttgacga cgtacaagca tttgatcatt taaatcttct
     1741 acttgatgtt gttgttcctg tgtcacaaaa gttcctccat tctcatgtta atctctttcc
     1801 tataatgcca atgaaacaca aaaaaagcaa gccgaacttg gaatttcggc ttgaaaattc
     1861 acgaaaaact tagaaatcac aacgtttgtt tcaatagtac tttttccgct ttttctacaa
     1921 attgatccag caggtcattg atttcttgct gtttttctgt ctgattgatt gctactttca
     1981 ctttcgctgc tcgaggaatc ccttttaaat aataagccgc atgtgttctg aattctctac
     2041 aagctacttt ttctcctttg agatctgcta agcgttgtaa atgaagttta gctgtagcaa
     2101 ttttttcggc tggtgttggt tcaggaatca gttctcctgt ttcgagataa tgctgtgtgc
     2161 ggtgaatcat ccatggattg cccagcgctg cacgaccaat catcacaccg tctgcaccga
     2221 catagtcaag catccatttt gcatcttctg gcgttttgac atccccgttc cccataaatg
     2281 ggatcgtcaa atgttttttc acttctttca atacttccca gtccgctttt ccttcataca
     2341 tctgaacccg tgtacgtcca tgcatagcga tggcacttgc tccagctgat tgtgctgcta
     2401 atgcattttc tacagcaaag acgtgctctt catcccaacc aatacgcatc ttgattgtta
     2461 ctgggatatt tactgctgaa gaaacagctt ggaccatttc gtatactttc tcaggatcta
     2521 aaagccattt tgctcctgct tctgctttga ttactttatt tactggacag cccatattga
     2581 tatctattat atcggcagct gtgttttctt cgacaaattg agcagcttca actagactat
     2641 ctttatttcc gccaaatatc tgtaaactta atggatgttc tgtttcttca atgtggagca
     2701 tctctaaagt ttttttgttt ctgaaatgaa taccttggtc actgatcatt tcacatacga
     2761 ctaagcccgc accaaattct ttgacagtga ctcgaaatgc tgaatttgtt attccagcca
     2821 tcggtgcaac aacgacacga ttagggattt ccacattgcc aattttccat gtgtgttcca
     2881 cttaaatccc tcctaaggct tctttcttca atccatctaa ttcttcttcg ctatacttgt
     2941 atttgttatt acagaaagaa caaaccgctt ctgcaccgtg atcttcgtct atcattgctt
     3001 ggatttcttc tgcacctaac gcgataattg cttctgcaaa tttttctttt gaacagtcac
     3061 atttgaattg aacaggcatt ttttccaaaa tgttcaattc ttctgtacca agcaatcctt
     3121 tcaatatttc ttctggtgtc tgtccttcat ctaagagggt agagattctt ggtgtcgttt
     3181 ttaattgttc ttcgatcttg ctgattgttt cttcatcggc acctggcatg atttggatca
     3241 tgaatcctcc cgccgtcttg atgctgtcgt cagtattgac caatacactc agtccaacag
     3301 ctgaagggat ttgttctgag atagcaagat agtaagtaaa atcttcagcg atttctccag
     3361 aaacaatcgg cgtttgccca gaaaaaggtt cttttaatcc taagtctttt gtgacagtaa
     3421 acattccttg tgttccaact gctccccgca catcgatttt accttcacta tttagaggaa
     3481 gactgagatg cggatttttg atgtaacctt tcacatctcc ctttccattt gcatctacta
     3541 caattcctgt tgctggacca tcgccttgga ttcgaacagt tagtttgtct tctcctttga
     3601 gcgtcgcacc aagtagcaga ctaccgacca atgtccgtcc caaagcagct gaggaggtgt
     3661 gccaagtatc atgacgtctt tgtgcctccg ctacggtatt tgttgcttga acggcgtatg
     3721 ctcggacgaa tccgtcgtga gcaagtgcct taaccaaata atctttcatt tgttatgtac
     3781 tcctttttcc gtttttaaac tcatagtgaa tcatactagc aactactgct tttttcaact
     3841 ttggaatgct tcttttaaaa ataattcaga atcatctaga aaacaggaca tcaccctatt
     3901 tacaacttaa ataaagaaat ctcccgaaaa ccggtttttg ttccggtttt cgggagtcat
     3961 atcagatcaa ttgacctata tttttttatt tgtattgatc gtctgaatga ttatcttctg
     4021 attgttgaga atctgaatca tgttctgctt tattttcctc attcagttca tgttttgctt
     4081 cttcaaaatc acgtttttct tccgcttgtt tttcagcgtc tttttcttcc aaagcacgtt
     4141 ttgcttcttc aaaagttttc gcttcttttt cgcttggata atcatttgac tcagtacctt
     4201 caggcatcac accgtgttca aacaatgact tgattgcttt ggcatccaat gtttcgaatt
     4261 ccaaaagttt ttcagcaatc agtttatgct gtgcacgatg agcttcgatg atttctcgag
     4321 ctttatcatg agcttccatc aagatttttc gtacttcttg atcaatctcg aaagcgactt
     4381 gttccgagta agcttttgtt tgaccatagt cgcggccaac aaacacttga tggtttcctt
     4441 catattgaac aggacctaaa cgatcactca ttccatattc tgttaccatg cttcgtgcta
     4501 gagctgtcgc ttgctcaaag tcattggaag caccagttga ttggacattg aagatgattt
     4561 cttcagctgt acgtccacca agtaagccta cgatttgttc aaacatatct tctttcgtca
     4621 tcaagaactg atcttctttt ggtaaagcga tcatatatcc gccggcgcgt ccgcgaggga
     4681 tgatcgttac tttgtggacg acccgagcgc gactcaatac taatccgaca atcgtatgac
     4741 cagcttcgtg ataagcaacc atttcgcgtt cttttttgct gattacgcga tcttttttag
     4801 ctggacctgc aatcacgcga tcttccgctt catcgatatc agaagcatcg attttctttt
     4861 tatttcttct agcagctact aacgctgctt cattcaagac attttctaaa tctgctccgg
     4921 caaaacctgg tgtctgttga gccacaactt tcaagtcaac gtcatctgcc atcggtttat
     4981 tccgtgcatg gactcgcaag attgcttcac gaccttttac atctggacgt ccaactaaaa
     5041 tctggcggtc aaaacggcct ggacgtagta acgctgggtc tagtacatca gaacggttgg
     5101 ttgcagcaat gacgattact ccttcatttc cgtcgaagcc atccatttct acaagcaatt
     5161 ggttcaatgt ttgttcgcgt tcatcatgtc cgccgcccat accggcacca cgttgacgac
     5221 ctaccgcatc aatttcatca atgaaaatga tagctggtgc gtttttcttc gctgtttcaa
     5281 acaaatcacg aacacgactt gctccaacac cgacaaacat ttctacgaaa tcagaacccg
     5341 agatcgagta aaacggtact ccagcttctc cggcaacagc ttttgctaat aaggttttac
     5401 ctgttcctgg aggtccttct aaaagtacac ctgccgggat acgggcacct aattcaacaa
     5461 atcggcgagg atctttcaga aattcaacaa cttcaactag ttcttgtttt tcttcttccg
     5521 cacccgctac atcagaaaaa cgtacgcgat tagctttttt gtctgcttct ttggcttttg
     5581 atttaccaaa gttcatgaca cggccgccac cgccaccgcc gccgccttgt tgtcccatca
     5641 tcatgtaaaa gaaaaagatg atgattacga ttggcaagaa gctaagcaat agggaaaccc
     5701 atataccgct gcttgattct tcttttactg ttgtagcaac tttgttttct tttgccagtt
     5761 ctgttacttg gctcaatgtt gtatcatttg gcaaaatgat tgttgtgaaa tgggtagtag
     5821 tcgtatctgt tgaacctaag atagctaacc caccgctgtt agcgacggtc tgctgttctt
     5881 tgtattcacc agtgatctta tatacaccat ttgcaggctg cacttcaaat tctttgatct
     5941 tgcctgcttg tagttgttct gtgaattttg agtactcgat atctgacgat cgctggccgt
     6001 tattaccaaa aatgaaataa acgaccatta ccatcgctaa gattacgagg acataataca
     6061 aggcattttt catcatgccg ttgttctttt tgttcatagt tgccctccct aagcgtcaat
     6121 ttaatttaac agttttttat caatcctgac cattctattg taccataatt taattgagat
     6181 acagcattaa tttgattgat aaacactagg tttcaataca ccgatgtacg gaaggttacg
     6241 atatgcttct gcataatcca agccataacc gaccacaaat tcatttggta cgttgaagcc
     6301 aacataatct gcttcgatat ttactactct tccttcaggt ttgtcaagta gtgtaacaat
     6361 tttgactgat tttgctttgc ggtatttgaa aagatccact aaataagcaa gtgttcttcc
     6421 gctgtcaatg atatcttcaa cgatcaacag atcgcgacct tctacattcg tatctaagtc
     6481 tttgacgatt tttacttccc ctgacgagac tgttgcattc ccgtagcttg aaacatccat
     6541 gaaatccaat tccaaatacg tatcgataga acgaacgata tctgccatga atggtaccgc
     6601 tccttttaag acacctacta ctaggggatt ggtgttttgg taaatttctg ttaactcttt
     6661 gcctagttct ttgcaacgaa cttgtatctc ttcttgtgag atcaaaattc tctcaatatc
     6721 cttttccaac atcaggttct ccttccaatt gcttcttttt gatatttgta aagaagtatg
     6781 tagtgtattt tatcagtttc ttcacgaata ctcaaatatg aaaaggcaaa aggtatcaag
     6841 ctatatatat ttcttttggc atctgtaatg atccaagcat gctgcctttg tgaattaggt
     6901 actttctgat cgataaaata tctagatact ttttttgtga gattctctgt taatttaatg
     6961 cggtctcctg cttttctttt ttcgacatac agttgcacat cagcaggaat ccaaatgtca
     7021 tgagaaaata gagaccatga atctgttgct ttgatgtccc ctgcattttc aggtttgacc
     7081 agaccaatcc attctgtttc attcaaatat attccttgtt ctggaatcag caacaagcgc
     7141 tggtcttctg tctggctagt tgcttgtttt tcttttttag agagtacaac aaaatgatac
     7201 tctcttttca gtatccaatt attgccaata ctgagttgcc attggctttt tttctgttgc
     7261 cactggtcta aaatcgactc catttgttgt tctttcaaaa taattcctgt ttttgagaat
     7321 gctttgtcga aaaaggagta aaaaaaatag tatcgttcag catctgtcat ttcttcaatc
     7381 atttctgcag aaaatcgaaa ttgttcctcc cactgttcga cttccttttc gatcaactgt
     7441 cccatatatt tttgaatgac ttggtctgcc cattggatct gttgcgaaaa ttgctggaaa
     7501 tgtctcaacg cctgtgtatt ttcttgtttc aattgaggaa ctaccaaatg acgaagccga
     7561 tttctctgga tatctaacaa ttggtttgtc tgatcctcaa agtaaacaag ctgcgattcc
     7621 gctgcataag tatataggtc ttctttgcta aaactcagca acggacgaat cagtctgccc
     7681 gtagcaaatg gttgtacttc tttgattcca gaaaaagtat ttaactgtcc cccacgaacg
     7741 attttcatta acaccgtctc gatttgatca tctccgtgat gagcagtcat caacgtgtca
     7801 tattgtcctt cttccatcac gcgagtgaat tcttgataac gaaagttacg agcttctgtt
     7861 tctatggcgg atttggctgg atcttcccaa atacttacat ataaagggag attcttctct
     7921 tcacaatatg agcgaagata ggcagcttct tctgctgatt cttctcttaa ttggtgattg
     7981 atgtggacga tacctaaaga aaaaccgatt tggcgttgca cttgtaaaag taaatggagc
     8041 aaaaccatcg aatcaacacc acctgatacc gctgctaaaa ttctcgatcc ttgttgccag
     8101 aatccttgtt cattcccgtg ccgaataaat gactgctgta atagcggtgc ttttttcccg
     8161 cctttttcaa cgttcatcct ttccacaccc tttcttccta aaaaaaagag agcgggaaaa
     8221 cgtctaaaac gacaattaac ctgccctctg ttgatactat gacccaaata gtcgcactat
     8281 cattatttgc ggctttttag ctgcgacgtc cgccacggcc accgcgcttt ccttctgtgt
     8341 ttcgcttcaa tgaagataag cggtcatcgc tatctttcaa aaatgaactc atcaatgaat
     8401 cgaagtcttc tttttgagct gaaggttgac tgcgagaaaa agattttttc tgtgctggac
     8461 gatttcggtt gtcacgggga tctttttgtt gatatttttt aggacggctt tcttctccag
     8521 attcagcagg ttgttcctgc gctttccgga tagatagtcc gatttttcca tcatcattta
     8581 ctgaaataac tttaactgtt acttcatctc ctacacttaa aacatcatgg atgtctttta
     8641 catagccatt tgacacttcg ctaatatgaa ctagacccgt ttttccagca cctagatcaa
     8701 taaacgcacc aaaatttgtg attcctgata cttttccttg cagttttgct cctacctcga
     8761 ttgacataaa aaaaatgttc ctcctaatta tttccctttt ttatattcct gcttattttg
     8821 cagttgtaga actagttgat tgatctgttt catttgtcga gctgtctgta tttgattcta
     8881 atataggata aactttttca ccgtcttttg agtaaaagaa tttgcttctg gcaagtttag
     8941 ctacatactc atcatctttc agcaatgaaa catcttggtg cagctgatca acgtctgcgt
     9001 tgacttcttt catttcagta tttgcttcga ctctcaattc gttcaattga tgcatccttt
     9061 gcatgtcatg gaaaatctgc acacctatta tcgcgaaaat gaccaaagca ccaacaaaaa
     9121 ttgcagccag tctgcgtctt cgaaaaatca gttgacgttg ttgttgctga aattcagcat
     9181 actttttctt tgcatattcc gtatctagcg cagcgatttt attgcttttt ttcgtcactt
     9241 ggattcgctc caatcttttt gattgtcttt ttcattatac caatatcgtg aaagcttgtc
     9301 taatctattt ctctagtgtt tctgtgcgat tttcagaaat aatttcgtac attttttgtg
     9361 catcttcttt tttggttgat tcatgtaatt ccgtaatttt cacttccaac gttttgttgc
     9421 caaactggac tttcaaaata tcgcctactt tgacatcact ggaagatttc gccaatttac
     9481 cgttgatttg aattcttcct ttatcagcta cttcttttgc tactgatcgt cttttgatga
     9541 ttcgggaaat ttttaaaaat ttatctaatc gcatatctta ttcctcactt tttaattttt
     9601 ttatttaatc gcaggatttt cttaccaaaa ggcaacatta gccattctcg tactgtaaac
     9661 agacgaagac gaatcactgc ataaatgaac acagaagccc cgatcccaac tcctatcacg
     9721 ctgacaaaaa aagcagtgga acgatgagtt acaggaccaa atactatatt caatacacca
     9781 taatacagca gcagtactac tgtcatacaa agaaggctga acatcaactt ccgtccaaaa
     9841 tgccgttctt tccaaaaagc atttagctct tttgtttcaa ttcgaacaaa ataccaaagc
     9901 gtcacaccta atcctaaaag ggtagacagg cttgcacctg ctgttcctaa taaaccggtc
     9961 agaaaagaag tagtcaatcc ttttaccaat agtccccatc ctgcgccttt taaagaaggg
    10021 cgaaacgagt tcttgctttg agcaatactt tgataagctt ggatgactgc agtgaaagca
    10081 atggaaaaaa caaataatac aagcgctgca tttcccgcat aatctttaaa caacgcataa
    10141 ttgatataag ggagcagcaa ggctaatccc aaagacgcag ctaatgctaa agcggtcgtc
    10201 aatctaagat agattttggc tgtctgtaaa aactgacgat agatccggtt ggtcaaatga
    10261 cgagtcaacg caggcaggaa tgtcgcactt aatgcagtgg caatcactaa acctaattgt
    10321 actaaaggct gacctcgatc atagacccct tttgctatct tcgcaccgta ttctgacagt
    10381 ccgtaaactt ccaaagcatt ttttacaaag aaagagtcga tcagctggaa aaagataagc
    10441 aatccactat aaatcgaaac cagtccgcct tctaccagaa aacgccggat caagggcttt
    10501 gacggctgtt tgaacaatgg aaaccgtcgg aagcttaacg ctcctccatg aatcttttgt
    10561 tcatagtagt gaaggatccc atatgcgcat aaaccgccaa aaacagctcc actcattgct
    10621 gccgtccctg tttggtacac cgtccatcca agctttctaa aagcaaacgc actacagaca
    10681 atcacaaaaa cacgaacgaa ctgctcaact acttgagaaa cagcagttgg ttccatcaag
    10741 aatcttcctt ggaaattccc tcgataaaaa gatagaccag gcatcagcaa aaaggtaaag
    10801 gaaactactt ggatcagcgg tttcaattgt atgtctccca tcatccaagc aatccagctg
    10861 ctaaatgcaa agacaaacaa ccacaaaaag acacctgtcc aaaatactaa gggataaaga
    10921 ttttgcaaag cttgctttcg ctcgaaagga ttctttcttt ctgcaacgta ctttgatatg
    10981 aactgcggaa gaccggataa agctaaagtc attgctatcc cataaattgg ataaacttgc
    11041 tgataaacat aaaaaccttc gtcgccgact aagttttgga ggggaattcg gtaaagggca
    11101 cttaatactt ttgctatgaa agaagcaatc gtcaatacaa atgctccttg catgacgcgc
    11161 cgcatttctt tatgatccaa atcattgctc cttcctacgc tgacagatat ttttgctcac
    11221 gcaacgcttt gacaaacttt tggatttcct gaagccatac tgcttcttcc atttggttgg
    11281 ggatcgtcaa gcgtatcacc atctgttctt tttcaacgcc tagactggct tttagttttg
    11341 tcgctgtcag tgcttcgaat aactgttcca cactatagcg ttttgttcca actttactta
    11401 gagtaaagac tatatgcgcc tcctgtttcc ttatcgtttc taggagcgct cgatcgccat
    11461 ccattttgat ctgcccaatt gtcaagagat gtgctacttc ttctggatat tctccaaaac
    11521 ggtcgagcag atcatcttct agttcatcta gcatttcttg cgaatcaagt tcacgaattc
    11581 gtttatagat ctcgattttc tgccgttgat ctgccacgta tgtttcaggt agatacgcat
    11641 cgacaccgag gtcgatctca acagtggtct tctccacttg actattcttt ccttgtttac
    11701 gagaaacagc ttcgcttagc atttgagaat acatatcgaa accgactgca tcaataaatc
    11761 catgctgctg agcacctaag agattacctg caccgcgaat ggaaagatcc cgcatcgcaa
    11821 tcttgaatcc agatcccagt tctgtaaaat ctttgattgc ctgaagacgt ttttcactga
    11881 cttcgttaag gatcttctgc tgctcataca taaagtaagc atatgcgaca cggctgcttc
    11941 ttcctacccg ccctcgcaat tggtataacg tggataatcc catataatca gcgttctcaa
    12001 cgaacaacgt atttgcgttc gggatatcta ctcctgtttc aatgatggtt gtagtgacaa
    12061 gaacatcata ttgaccttca ataaattcaa aaagcgtatt ttctaattgc gcctctgtca
    12121 tttgcccatg agcataaccg atccgtgctt ccggaacgag ttcttggatt tcttctactt
    12181 tttgttcgat cgtttctacc cgattatata aatagaatac ttgtccacct cgtcccattt
    12241 ctcgatggat tgcttcgcga atagccccag gatttttttc cataacatac gtttggatcg
    12301 gataacgatt cgcaggcggt gtttcgatta ctgaaagatc ccgcacgcct aacatcgaca
    12361 tatgcaaagt tctcggaatc ggtgttgcag tcagagtcaa aacatccact tgtgcacgta
    12421 actgtttcaa tcgttcctta tgttttacac caaaacgttg ttcttcgtca atgatcagta
    12481 gacccaaatc gctaaattca atatcttttg acaaaattcg gtgggttcct acaacaatat
    12541 caacttgacc tgtacgcagt tgttcgatgg tttctttttg ttgctttctt gttctgaaac
    12601 gacttaatag cccgatattt acggggaaac cttcaaatct ttccagcatc gtttcatagt
    12661 gctgctgtgc caagatcgtt gttggaacta aaaaggctac ttgtttgctt tcctttatcg
    12721 ctttaaaggc cgctcgaagc gctacttctg ttttcccata accaacatca cctacgagta
    12781 gtcgatccat tggtttttct ttttccatat cccgtttgat ctccgctgca cttctcaact
    12841 ggtcatcagt ttcagagtag gggaaggcat tttcgaattc tttttggtag gcgtcatcag
    12901 gtccaaaagc atatcctttt tctgactctc ttgcggcata taatttgatt agatcgtcag
    12961 cgatatcctc gatttttgaa gagacttttc gcttggtttt cgtccattca cttccgccta
    13021 gcttattgat gcgaggcgct ttggcttcag aggcgacata tttttggatc agattcagtt
    13081 gagtaacagg aataaacagt ttgtcgtcgt tttgatacaa aatagtcata tagtcttggt
    13141 gaacgccatc tacttctagc gtttccatcc caatgtattt cccgatacca tgattggcat
    13201 ggacaacgta atctcctgct tttaactcat tatagctttt caatcgctct gcgtttgtta
    13261 ctgtttgacg tctcgtttgt tttttcgtcg ttttttggaa aatttctttt tctgtgatag
    13321 ctactatttt ttcttgagga agttcaaaac cagcctgcaa agctccttca actaattgta
    13381 cctgtcctct gatcaatgca tttttttctg tttctactac taatatgtct tcatctcgaa
    13441 gcatttgttc tgctttgcgt attctgtcct ttgtcggaat aaagatcaca acagtctgtt
    13501 tttgcttgcg ccaacggtcc atttctgttt tcagcaatgg catttgtccg aagaactgtt
    13561 gcatcgtccg atattgaaaa ttatggattg cttggaaacg aagatttccc atcccttttt
    13621 ggaagagtga gaaaaaggtt gtggtgaact tttccttctg cacgattttt tgtatatctg
    13681 ctgaaaatgt ctgctcagaa aacacccgca attcgctgat tttttgagtc tgccattctg
    13741 ccgcttccct ttcgatttcc cgatttgttt ccatgattcg ctgataatca tcaacgacta
    13801 gtaaactatt tgaagggaaa taatccaaga tcgtcacaga ttcttggtaa agaagatctg
    13861 agtagaattt tgcttcttct gtcgggacac ctgcttgcca gcttgaagtc agctgaccga
    13921 agtaatcttg caaaaactcg cggtctgctt cttctgtggt cgcagcaagc cgtttttcta
    13981 gcattttttg catttgctct attccatgtt ctagatcctc tggtccgtag acttgttcac
    14041 tcgttgggga aacccagatt tcttccgttt tttcgattga gcgttgtgtt tctgcttcaa
    14101 aataccgcat agaatctatc tctacatcaa atagttctac acgaacagga taggttgtcg
    14161 ttaacgggta aatatcgata atgcttcctc gaatactgaa ttctcccggt tttgcgacca
    14221 tctcttctcg tacatagccc atcaaagtca attggcgagg aagattttcc acgtctatct
    14281 catcaccaat atgccaattc aattgcgcct gtagccaagt tttcttattc ggcagtcgtt
    14341 tgtgcaaaga tgcgactggc aacaaataga tacctgcttt tttttcttga agtgcgttaa
    14401 gcgttgcaac tctctcgcct cgcgcttctg gagaagaaaa ggccatttca gcagaaagca
    14461 cttcatccac tggaaataaa tggatctgat cttgcggaat cacatgttgc agatcttctg
    14521 ctaactgatt cgcataatac aaatttggca tcagaataat catgttttta tttgttttct
    14581 taaacgctcg agcaaataga agtgttttcg ctgagcccgc taatcctgtg attagttgtc
    14641 taggcatttt ttcttctaaa ttgctgaccc attcttttac ttgcggatct tttccgacta
    14701 attgaataat atccattcct tttcctcctt cctcaattga atcgattcat cgcatccacg
    14761 aacgaatgcc cttctatgag atattcgact gctttaacag attgacacat gctttcttct
    14821 atcaaaggct gattttcttt gctgaaagga ctaagcacat gctggactac tgtttttttc
    14881 ccttctggcc tgccgattcc caccttgatt cgatcaaaga cattcgtatt caaatgggaa
    14941 ataatacttt tgataccgtt atgcccgccg gcacttccct tttgacggag ccggattttt
    15001 cccactgcca gatccaaatc atcgtagatt acgactaatt cctctggata aatcccaaaa
    15061 taagtcatta gtgggccgac tgcacgacca gattcattca tgaatgtttg tggtttgact
    15121 aaaaggatct tttctccatt atgaaaaaat tcggcaactt ctgcttcgaa cggatttttt
    15181 ttgaaagtag cttgatgctc gcgtgcaatt ttatccacga ccataaagcc gatattgtgt
    15241 tttgtatatt ggtatttggt acctgggttt cctaacccga cgatcatttt catttttgtt
    15301 ttccacctaa ttctaaaaag cagcacaaaa ggctggactg ctaattagcg gtccagtcca
    15361 tcgagctttt ttctttttta tacttgtcaa tgcatagtat aacacaaaac aaggcgtttc
    15421 ggctaaaaga gaatcgttaa taaatgataa ataaattaaa aatttcttca aagtttaatg
    15481 aaataattga tatataaaaa tagtgaaata acgcacatgt aagcttttac atataataca
    15541 accttgttat ctgttatatt ttatagccaa atagcgtgac atggggcttg agaaatgtta
    15601 gcataagcat gggaaaaaac aaatcaatgg aaggattgat actaaatgac tgcagctgca
    15661 gaaaaaaaag atcatcaaaa agttattctt gttggagacg gcgccgtagg ttctagctat
    15721 gcctttgctc tagttactca aaatatcgct caagaagtag gtattgtgga tatcaatact
    15781 gctaaaactg aaggagacgc aattgacttg tctcacgctt tggcatttac ttcacctaaa
    15841 aaaatctatt ctgctactta cgcagacgca catgatgctg acttagttgt tattacagct
    15901 ggcgcaccac aaaaaccagg tgaaactcgt ttagacttag taaacaaaaa cctaaaaatc
    15961 aaccgcgatg ttgtcacaca aatcgttgaa tcaggattta acggtatctt tttagttgcc
    16021 gctaacccag tagatatttt gacttactca acttggaaat tctcaggttt ccctaaagaa
    16081 cgcgtaatcg gttcaggtac ctctcttgac tcagctcgtt tccgtcaagc acttgcggaa
    16141 ttagttgacg ttgatgcacg taacgtccat gcttacatct taggtgaaca cggagattca
    16201 gaattcccag tttggtcaca tgcaaatgtt gccggcttgc aaatctatga atgggtaaaa
    16261 aacaacccag aaatcgatga agaagctatg gttaacctat tctttggcgt acgtgacgct
    16321 gcttacacaa ttatcgagaa aaaaggcgct actttctatg gtatcgcagt agcattagca
    16381 cgtatcactc gtgccatctt agatgatgaa aatgctgttc ttcctttatc agtattcatg
    16441 aatggcgaat atggattgaa cgacatctac attggtgcac ctgctgttat caaccgtcaa
    16501 ggtatccaaa aagtaatcga aattccttta agtgacagcg agcaagaccg tatggctgct
    16561 tctgctaagc aattaaaaga aattctagat gaagcttttg ctaaactaga cgctgaataa
    16621 tctttgaaca tgacggaaaa catctattcg gtgttttccg tttttttatt ttatcaccct
    16681 tatggtttct aaattttttc ataaagtaga gataaccttc gtcttttcct ctaaattatg
    16741 ctatcatgag accgttccac cctaaacttt tacttaagga gttgtcgata tgacaagatc
    16801 ttcgcatcga aaaaaatcga aacatgttgc tttgctttca gttcttggtg ttctcttagt
    16861 gctgatcagc ttttatacct atcgcagtac atactacaca aagcattttt tgccgaatac
    16921 agaaataaat ggaataaatg tcagcaatct aacagtcgaa aaagcaaatg aaaaattaaa
    16981 agaagcttat tctgataaaa aactggctat taaagaagat ggaaaagtct ggcaggaagt
    17041 accaaaatcc gagctcggct acaaagatga tttcacttcg gagctttctc cgatcctaaa
    17101 tgaacaaaac ggatggactt ggggaatgac ttatgtctca gcggcagaaa aacaagaaat
    17161 agatcctgtc agtcaaaacc accaaaaact agacactacc gttcagacat tgacaactaa
    17221 gctgactgat ttgaacaaag accggacacc tactactgat gcaacgatcg aaaaagcagg
    17281 tgactctttc gttatcaagc ctgaagtcaa tggaaatacg atcgacgtcg atgctgcggt
    17341 caaacaatta aaaagtgcgg taaactctgg taaagataca attgaattga ctgaattcaa
    17401 agaaaaacca aaagtaacat ctgaagacag ctcactgaaa gaacaactcg catccatgaa
    17461 tgctatcgca aatgtaaaag ctacttacag tataaacggt gagactttcc agatcccatc
    17521 aagtgatatt atgagttggc tgacctacaa tgatggaaaa gtcgatttag atacagaaca
    17581 agtacgtcaa tatgtaacag atttaggcac aaagtataac acaagtacga atgacacaaa
    17641 atttaaaagt acaaaaagag gagaagttac ggtcccagtt ggcacttaca gttggacgat
    17701 ccaaacagac agtgagacag aagcattgaa aaaagcgatt ctggcaggcc aagattttac
    17761 tcgttcaccc attgtgcaag gtggcacaac cgctgatcat ccattgatcg aagatacgta
    17821 tatcgaagtt gatctggaga accagcatat gtggtattac aaagacggaa aagtggccct
    17881 tgaaacagat atcgtttccg gaaagccaac tacacctaca cctgctggag tcttctatgt
    17941 ttggaataaa gaagaagatg caactttaaa aggaacaaat gacgatggaa caccttacga
    18001 gagtccggta aactactgga tgccgattga ctggacaggt gttggtatcc acgattcaga
    18061 ctggcaacct gaatacggtg gagacttatg gaaaacccgc ggttcacacg gctgcatcaa
    18121 caccccacca agtgtaatga aggaattatt cgggatggtc gaaaaaggaa cgcctgtatt
    18181 ggtcttctaa caaaaataat atataaaaag aaagcacccg tacgattggt acaggtgctt
    18241 tctttttatt atctaacaat gtgctactta catttcagca gcagtaaaga cttctgaaac
    18301 atcatcatcg tcttctagct tatctactag acgttccaat tgctcttttt gttcctcatt
    18361 taaatcaatc gtcgtttgtg gaatcatcgt caattctgct tgcgccaaag tgaactcttt
    18421 ttccaactca tctcgtacag cagcaaaatc ttctggtgca gtataaatct cgaatacttc
    18481 aggagaagtt tgcagatctt ctgcccccgc ctctaataca tcctcaaaca tcgtctcttc
    18541 atcaacagtc aacccatcgc gttcaatagc gatatatcct ttgcggtcaa acaagtaact
    18601 gactgaaccc gtttctccca gtgatccacc gtttcgagta aaagctactc ggacatttgt
    18661 cgctgtccga ttacggttat ctgtcaatgc atggactaag attcctactc cacccggacc
    18721 atatccttca tacgtgattt catcgtaatt cgtttcatct acggcactcg tagccttttt
    18781 aatcgcccgt tccacattgt cgttaggcat atttgcagct tttgctttgt ccacaacaag
    18841 ccgcaaagct ggattcatag atggatcagg accgcctgct ttagctgcca tataaatttc
    18901 acgagaaagt ttctgaaaga ctttccctcg ttttgcatct tgggcatttt ttcttccttg
    18961 gatattatgc catttactat gtcctgacat gatttttcct cccttgttct tttgagaata
    19021 cacttctatt ctagtactat ttcacaaaaa aacaaagcca cagccaatct ataaacgctt
    19081 cttgaatgcc gcaaaaagca cacataatat cacagcagtc agagcaccga tcgaatccaa
    19141 catgacatct tggaacatag gtgttcgtcc accggtcatc atctgatgga attcgtccaa
    19201 acctgcgtac cctgttgctg aaagccaagc aaggatcgct gtcagccacc aaatttttag
    19261 cttggggaaa acacctaaaa acaagctgcc tcccaaaata aaataggtac cgaaatgtgc
    19321 gcccttcctt aaaaagaact ctaaaaattt ggcgtagccc agatggtcga tactcaccat
    19381 acttccacca taattaaaac gtatacccgc taatgtgtct ttaaatggct gattaggcag
    19441 ccagctttct aatcgcgaaa cttgtgattg ctgtccgtaa gtttgagaag aactgtaaaa
    19501 taagattata acgactatca gtgcgattac taaataaata tttccgtttt tcatttgttt
    19561 ttttgaagaa ttcataagtc acctctgtat tttatcttag actttttttg aagaaacaac
    19621 aatcattttc atagagactt cttccataaa aaagtgctat acttaatttt gtattacaat
    19681 atgaagggag ttttttcatg agttattcat taaattggaa tctagattct attttttccg
    19741 gaggcagtca ttctgatgct ttgaaccaac ggatgaagca attggaagac caaatgaacg
    19801 agtattatca ccgcgtaaca aaatggtcac ctagttctga caatgcagag cagttaaacg
    19861 ctattcttca attgcaagaa acgattacga atggttttac ccaatgcagc agctatatta
    19921 ctgcattgct ttctgccaat gtcaatgatt cagatgcaaa ggtcttatca ggcaagcttt
    19981 acgcaatgct gcctcgtctg caatcagccg aaactgtatt gtcaaagaaa tttgcagaaa
    20041 tatctgacaa tgattggaat cagatgcttt ctcaatcttc gtttgaaaca attgcttttc
    20101 gtttaaacga gattcgtcgt gatggctcac aattattatc agaagctgaa gaaaacatta
    20161 taaatacgct ttctttagat ggtctaaatg cttggagcag tcattatgat acgattgtag
    20221 ccagcatttc gattcccttc gaacaagatg gacaagtagt cgaattatct gctggacaag
    20281 ccttcaataa gatgatgggc gatccagatc caaaagtcag agaaacactt ttcgcagcat
    20341 gggaagaagc ctgggaagaa aaggcacccc tcttcacgga tactttgaat catttagatg
    20401 gttttagact ttctgactat gaacttcatg gtgtcacaga ttttctacaa aaaccattag
    20461 agtacaatcg gttgaagaaa gaaacattgt ctgtcatgtg ggatacaatc cagaaaaaca
    20521 aacaacccct tgttaattac ttgactagaa aagctaatct tttcggtaaa gaaaaaatgg
    20581 aatggcaaga tcaagatgca cctattattt taggcgattt gaaagaaaaa acctttagtt
    20641 ttgacgaagc agcagcattt attatcgaaa actttaacaa attcagtcca aaaatggcta
    20701 aatttgccca atctgctttt gaaaagagct ggatcgaagc agaagatcgt cctgggaaac
    20761 gtcctggtgg ttattgtact gagttaccag aaacaaaaga atcaaggatc tttatgacct
    20821 acagtaattc tgttaatgag gttgcgacac ttgctcatga actaggacat gctttccata
    20881 gcagcacgat gtgggattta ccttctttaa atcgcgaata tgccatgaat gtcgcagaaa
    20941 cagcaagtac attcgcagaa ctgatcgtag ccgatgctac attaaaagtc gcaaaaacaa
    21001 aagaagaaaa aatcaatttg ctagatacaa aactgcaaaa tgctttagct atgttcatga
    21061 atatccattc ccgattcatt tttgaaaatc gattctatga agcacggcaa gaaggattag
    21121 tctcagaaga taagatcaca gagatgatgg ttgaggcgca aaaagaaggc tatcaaggag
    21181 ctttagctac ttaccatcca tatttctggg ctgcaaaatt gcatttcttt attgatgacg
    21241 taccgtttta taacttccca tacacatttg gctacctctt tagtttgggc atctatgctt
    21301 acgccaacca aaagggagca aattttgaag aagaatatat ccatttacta cgcgatactg
    21361 cctcaatgac gacggaagat cttgcgaaga aacatttgaa tgtcgatctt acccaaccag
    21421 atttttggca agcaggtatc gacatggtga ctgaagatat ccaaacgttc atggaattga
    21481 cagaagaatt tatctgattt ctattttatc acaa
//
LOCUS       DACEUZ010000035        21090 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.035, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000035 DACEUZ010000000
VERSION     DACEUZ010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21090
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.035"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(88..858)
                     /locus_tag="IXG44_001144"
     CDS             complement(88..858)
                     /locus_tag="IXG44_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1657876.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(851..1786)
                     /locus_tag="IXG44_001145"
     CDS             complement(851..1786)
                     /locus_tag="IXG44_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1657877.1"
                     /translation="MANALEINNLKKIYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDKDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYDTKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(2380..4029)
                     /locus_tag="IXG44_001146"
     CDS             complement(2380..4029)
                     /locus_tag="IXG44_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAQ1657878.1"
                     /translation="MGQREFIVLIIIAVVILLVVLDIFSRLKVKETVRSNWGKIPYQP
                     RFDKEESLKDAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDYQLEKLIAFYQENPQLRERIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGVGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKMTTPLQKELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAVLNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLNYPKSIVEQAQKESLLFDEQRQWYPFD"
     gene            complement(4224..5120)
                     /locus_tag="IXG44_001147"
     CDS             complement(4224..5120)
                     /locus_tag="IXG44_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ1657879.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWVDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDSFIRLTVANPIKPGAQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(5113..5793)
                     /locus_tag="IXG44_001148"
     CDS             complement(5113..5793)
                     /locus_tag="IXG44_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1657880.1"
                     /translation="MFLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(5794..6576)
                     /locus_tag="IXG44_001149"
     CDS             complement(5794..6576)
                     /locus_tag="IXG44_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ1657881.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMIAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LILATLSVPVALTFITRSTSVSLLLSFLYIMGTAFVPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(6569..7486)
                     /locus_tag="IXG44_001150"
     CDS             complement(6569..7486)
                     /locus_tag="IXG44_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1657882.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(7489..7773)
                     /locus_tag="IXG44_001151"
     CDS             complement(7489..7773)
                     /locus_tag="IXG44_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1657883.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWQTTQPRLRDSLIFLEIQLENLHFLYTFLAAFFIPVNYTSLINKKEG"
     gene            8561..11788
                     /gene="fss3"
                     /locus_tag="IXG44_001152"
     CDS             8561..11788
                     /gene="fss3"
                     /locus_tag="IXG44_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HAQ1657884.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVALVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            11901..12215
                     /locus_tag="IXG44_001153"
     CDS             11901..12215
                     /locus_tag="IXG44_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ1657885.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            12228..12602
                     /locus_tag="IXG44_001154"
     CDS             12228..12602
                     /locus_tag="IXG44_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ1657886.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            12612..12947
                     /locus_tag="IXG44_001155"
     CDS             12612..12947
                     /locus_tag="IXG44_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HAQ1657887.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            13030..14175
                     /locus_tag="IXG44_001156"
     CDS             13030..14175
                     /locus_tag="IXG44_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAQ1657888.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQCATCF"
     gene            14890..16749
                     /gene="ltrA"
                     /locus_tag="IXG44_001157"
     CDS             14890..16749
                     /gene="ltrA"
                     /locus_tag="IXG44_001157"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAQ1657889.1"
                     /translation="MNKVKTNKTLRHSEYYGMTQIFDELYVKSDKKEVFTELMTIIMS
                     DENIRLAYRNIKNNKGSYTASTDKKSIRDIKTLSEDTFLTEVKVLFRCYKPRAVRRVE
                     IPKPNGKTRPLGIPSIWDRLIQQCILQVLEPICEAKFHNYSYGFRPNRSTHHAIATCN
                     RLANRGKLHFVVDVDIKGFFDNVNHSKLLRQMWTIGIRDKWLLGIIRSMLNAPIVLPT
                     GKVEHPKKGVPQGGVLSPLLSNIILNELDWWVASQWQNTTIETEKIMDRRDKGKGIDK
                     TNQYRALRRTNLKEIYIVRYADDFKIFCRKRSHADKIFHATKMWLKERLSLEISEEKS
                     KIVNLKKGKSEYLGFELKLIKKGKKYVVESHMSKKSMTRVKIQLKKQLRKVARPKNHC
                     EQAKEIGLYNSMVIGIHQYYGIATCVNLDCSKIAYTLKPFINHKLPTKKHGKILNTFI
                     KSKYGKSKEMRWLNNVPIVPLNYVQFRLAVPLSEGTCKYTESGRSKIHSKLKLDLALL
                     LHMMRNSNYERSIEFVDNRISKYSAQKGKCAITGKFLEYEEIHCHHIVPVKQQGTDKY
                     SNLIIIHKNVHALVHAIEEKIIHKYLNVLNLTNEQIEKLNELRVKAGNSVLTV"
     gene            16843..17082
                     /locus_tag="IXG44_001158"
     CDS             16843..17082
                     /locus_tag="IXG44_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HAQ1657890.1"
                     /translation="MTSKDYLSLFNFRVALGRMSELGYSMMFGEVEKNFFMKHIKGRG
                     YVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            17160..17834
                     /locus_tag="IXG44_001159"
     CDS             17160..17834
                     /locus_tag="IXG44_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ1657891.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            18067..18501
                     /gene="dcm"
                     /locus_tag="IXG44_001160"
     CDS             18067..18501
                     /gene="dcm"
                     /locus_tag="IXG44_001160"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HAQ1657892.1"
                     /translation="MRFLDLFAGIGGFRLGMEQASHHCIGFCEIDKFARRSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQRFSDTRGTLFFEIARF
                     TTLLRPKLLFLENVRGLLNHEGGATFETILRTLDGLGYDVEW"
     gene            18502..19209
                     /locus_tag="IXG44_001161"
     CDS             18502..19209
                     /locus_tag="IXG44_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HAQ1657893.1"
                     /translation="MLNSKAYIPQSRERIFLIGHSRDACTEQVFPIIGPSSTSDQNIR
                     NLVNINPSNRGMGGQVYGSDGVAPTLTGDEGIKIALPARDGISVAGLLPGKFEQGNRV
                     YEVTGTAPTLSTKQGGTKIMIRKNETEYQEVKPEDSVNLAFPNSTSRRGRLGKQSVHT
                     LLTGDQQAIVTDQYQIRKLTPRECWRLQGFPDWAFDRDSQVNSDSQLYKQAGNSVTVP
                     VIFDIARRLKELDVHGL"
     gene            19199..19489
                     /locus_tag="IXG44_001162"
     CDS             19199..19489
                     /locus_tag="IXG44_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1657894.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            19748..20932
                     /locus_tag="IXG44_001163"
     CDS             19748..20932
                     /locus_tag="IXG44_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ1657895.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            20947..21066
                     /locus_tag="IXG44_001164"
     CDS             20947..21066
                     /locus_tag="IXG44_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HAQ1657896.1"
                     /translation="MGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKGK"
BASE COUNT         6858 a         3944 c         4216 g         6072 t
ORIGIN      
        1 gctcaaaaaa caacaaaata tgaggaacta ttttcccgtt tgtttctcta atactcaggt
       61 caaaacgctg atttcacaac ctcttttcta acttctcaat cctcttcctt tattgatcaa
      121 ataccaagta acggcgtaca aaacaacgat gaataacaca agtacgatca tcgatgttgc
      181 tactggcaca tcgatcgttc caaggaatcc gtaacggaaa ccggagatca tataaacgat
      241 cggattgact tttgaaacag cttgccaaaa gggcggtaac atagagatgg catagaaaac
      301 gccacctaga taagttaacg gctgcaagac aaatgtaggc acgatcgata catcatcata
      361 agactgagca aaaatcccgt tgagcagtcc tgctaatgaa aacaagattg ccgtcatcaa
      421 taatgtaatg atgaccatga accatgaata cacatgaagt ggtacaaaga acaaggaaat
      481 gatcgttacg agagagccga cgagaatact gcgacccaca ccaccaatca caaagcccca
      541 aataatcaca tgcgtaggaa caggtgctac caagatttct tcgatatttt tctgaaattt
      601 ttgagagaag aaggacgaag aaacattggc atacgaacta gtgatagcag acatcataat
      661 caaaccagga acgatgaatt ccatatatga aaaaccaccc atgtcaccga tccgcccacc
      721 gatcattttt ccgaaaataa caaaatacaa ggatgtcgtg atcactggcg gaaccagtgt
      781 ttggacccat atacgcaaat agcggttcgt ttccttgata gctaaacttt ttaatgcagt
      841 aaaataaaga ttaaacattt ttctcctccg tatgattatt ttcttctgtg attttcaaga
      901 acagttcttc taaacgattc gacttattcc tcatagaaag aactttgatt ccttgtcttg
      961 taagctgatc gaagattccg ttcacccctt gatttcgttc aacttcgaca gcgattgtct
     1021 gctcatcttc aaatgtacaa gcatatcctt caatcacagg tttggtatca tatggcgcta
     1081 aatcaaagat aaatgtttca aattgcagtt ttgacagaag ctctttcata cttgtattct
     1141 cgatcagttc acccgattgg atgattccga tgttgcgaca aagcatttca gcttcttcta
     1201 gataatgcgt cgtcaaaatg atcgttgtac cattttcgtt caattctcgt agaaactccc
     1261 acatctcgcg tctcaactcg atgtcaactc ctgctgttgg ttcatccaag atcagcaaac
     1321 gtggttcatg catcaatgcc ctagcaatca tcagtcttcg cttcattccc ccggaaagca
     1381 tacgtgcacg gacatttctc ttttcccaaa gattggactg cttcaaatat ttttcgctgc
     1441 gtttcatcgc ttctttgcga gagacaccgt agtagcctgc ttgattcacg acgatttgct
     1501 ggaccgtttc aaaagggtta aaattgaact cttgtggaac caatccaatc tgttgctttg
     1561 ctcgaactag atccttgtcc agatcataac cgaagacatt cacttttcca gatgttttat
     1621 tgactaaaga agtaataatc ccgatcgttg tagacttccc agctccattt ggccctaata
     1681 gcgcatagaa atctccttct ttcaccgaaa gatcgattcc ccgcaatgct tcgaccccac
     1741 ttccatagat ctttttcaaa ttatttattt caagcgcatt tgccatttat ttcctatctc
     1801 cttatagaca tgattcttaa ttgtatcatg gaatacagca gatgaaaaaa caacttggct
     1861 tccacacaaa aacacacctg ctaacaaaga ggagatgtgt tttctagttc atgatcatct
     1921 tcgatagaat aactatctaa acaactctgg aacttttaaa atcataaaag taccgacagc
     1981 aactactacg atgacatata gaagatcctt ataactgagg ttcttgtctt ttttattcat
     2041 gttaagcttt cctccgtcct tttatcgaaa gcttttttct tttattttaa ccgaaataaa
     2101 taaaatggaa atagttattt tcaatttctg ttttcgctgt ttgttttacg gagaactttg
     2161 ccatggaacg tgcgtctcgt cgctgatgct aagttttttc gataaaagct gctagaagga
     2221 agacagcgtg gtttattttg tatcgtcttt tgctaaatct tccaagatca tctggacagc
     2281 agactcgttc ccattgaatt ctgccacttt tttggcatca atgtacttct tttgataatc
     2341 gtctttttgg acccacgcat catgcttcgt ttgataaagt taatcaaacg gataccactg
     2401 acgctgttca tcgaacaaca aactttcttt ttgggcctgt tcaactatgg atttaggata
     2461 attcaaaact ttcagcaagg caatcgcatt tcgggtcctt gaaggtcctt cttttaattt
     2521 gaaatcaaaa ctgattcctt ttccttctgt tacttgttct tcaaagtgga cattctcaca
     2581 ataattcttt aaaatctctg tcaattcgat atcatgtgtg gcgacaaatg ctaaagatgg
     2641 atactccgca agccaccgaa cgactgaagc ggaagaagca atccgttcga tcgtatttgt
     2701 tccttttaaa atctcatcta cgaagcagta gcagcgttct ttggaagcca cttggtccag
     2761 caaacgtttg atcgatttga tttctgaaac aaaataactg tcgccttcaa ataaatcatc
     2821 ttcgattgcc atggaagtca acacatgtcc aggttgcagg gtaaactgtt ctgcaatcgc
     2881 tgtttggatc gtctgtgcta gaatacagct gatggctata cttttgacat acgtagattt
     2941 tcctgatgca ttggaacccg tgactaatgt attcttctgc cagttgactg gattaactac
     3001 cgcttctttt aacaatggat gatagatatc tttcgctgtc acaccgccct cagtaaactc
     3061 tggtcggcaa gtgatcggca tataggtgcg ataattcaaa acggctgctg caacttccaa
     3121 ttctcccagc aattcccaca gcttgatggc ttcagttgaa tgcttttcta atttagtgag
     3181 aacgaaatga taggagataa atggcagcat gaacatgatg ttgacatatt caaaaatcaa
     3241 ttctgcttca ctcccgctct tcattcgaaa tgaaaaacct aatttaggga tgtctcttaa
     3301 tggtttagtt tgctgtttca gctctttttg aagtggtgtc gtcatttttg ctaactgatt
     3361 ggcggctgaa atcgttgaaa ccaaataact catgctattc agctctgttt ctagtgtttg
     3421 tttcttcact agataataga ccgtattgaa tactaagcca ccgattgcta gatagattcc
     3481 tagtacttgc cctaaaagaa gcaaacctac acccaccagc ggaagcaagc caagaaaaat
     3541 aaataaacct acgcgtccaa tctttttact ttttccatcc gctagatatt gttttgcaaa
     3601 attatgatct ttttttccta aacgagcgaa ttgatattgg attctttctc tcagttgagg
     3661 attttcctga taaaaagcaa ttagtttttc taactgataa tcttctccaa aatcaaagct
     3721 acgtaaacgc tgatacaacg cttctgaacc tacactagaa taggtactgt tgatcccttc
     3781 aaatacttcg aacatatcca agtcatacca agtaagatcg tcgatttcac tatcccatga
     3841 acggaatttt ttctccgtga gccacgcatc cttcaaactt tcttctttat caaatctcgg
     3901 ttgataagga atttttcccc aattactacg aactgtttcc tttactttca aacgtgaaaa
     3961 aatgtctaaa acaactaata aaataacaac tgcaataata attaaaacga taaattctct
     4021 ttggcccata tccctctccg ttccttgatt tgttagtttt agtatagcac tgttgcgcta
     4081 tttgaaaagg gggtacaaaa ataactgtct tcgttaccta attcgtatca atcacaccaa
     4141 aataaaaaca ccttccaaaa aagaagatgt ctttaaaacg agatacaatt ttttgtcatt
     4201 cacttcaggt aaattagagc caattaaacc ctccgcaact tgacggagat ggagaagatg
     4261 cccccgtcta atctagcggt catctctcct cccattcctt ccaccaattt ttttgacagg
     4321 taaagcccga ttccgctaga ttccgtattt gtgcggctgg tattttcggt ataaaaacgc
     4381 tggaagactt tttcaacaga ggtttgcgct cctggtttga taggattggc cactgttaat
     4441 cgaatgaatg aatcctcttt tgaaaatgtc acctttgctt gtccatctgc ataacgcaat
     4501 aaattctgta tgatattttg cagcactcgc agcaacatga gcggatctgc taatacagtt
     4561 gtatttatcg agtgttcagc gaacgctacc tcgatatttt tctcagaaaa ttgttcataa
     4621 aaagagaaaa atacttcttc cacctcttta gaaaaagaaa gcgtatggag atccatttct
     4681 ttttgcttcg tctctattcg agcaagatca taaaactgat ccgtcatact gagtaatctt
     4741 gcaacagatt gtttgatgat cgttaaatac ttctcccgtt tctccgtatc ctctacctgt
     4801 tccagtaatt gtacataacc gttaatagca gtcaaaggag ttctgagatc atgagaaagg
     4861 ccgacgatcg atagtctgat gttttcttcc atttcttgaa atgcttgtcc tttttgttca
     4921 ttttcatcca cccatgtatt cagtgcatct actaggtcca ttagattctt ttctcgaaat
     4981 tcgactgaaa gacgaccacc atattcttgt cgttcagaaa gagtggcgat tttcttagtt
     5041 aatctcttga tttccctttg caggaagaaa ataaaacttc ccataaagat aacaacgaga
     5101 agcaacagcc acttaatcaa atctaaaccc cactccccac accgtatgaa tatacacagc
     5161 atgtgccttt gctagtttcc ctcgaagatt ggaaatatgc atattcactg ttttctcact
     5221 atcaaaatat ggttcttgcc aaactgtttc ataaagattt gctcgcgaaa atactttttt
     5281 cggatgttct aaaaaaagct tcagcagatc gaattctctt cctgtcaaat ggacagccac
     5341 accattcaca tttacatccc tcgactcttt gtccagtaag atttccttgt aatagatgcg
     5401 ttgcgacaga tcttttactt ggacagaagt ctgatgacga agttggatgc cgattcgtaa
     5461 caacagttct tcgatatcaa atggtttcgt cacatagtca tccgctcctg ctgccaacaa
     5521 ggaaacttta tctctttgat cgctttttgc tgaaagaacg atgatcggaa cattcgatgt
     5581 ctgtcggatt tctgctaaca atgcttcacc tgaaagccct ggaagcatga gatctagaag
     5641 gatcaaatcg acaggatatg tgcttaacaa taattttcct tccgtacctg aataagcatc
     5701 aagaagtttg tatctctgtt caagaacctc ctccagtaaa gaatgaacag cttcatcgtc
     5761 ttcgataatc aaaatagttt ccagcaggaa cacctactta atgtcagatt tttggaaaat
     5821 aaacattcct agagctgatg ataccacgat tgttactaga ctgaccagaa tcattttcgg
     5881 tgcttgcgaa taagttgctt gttgcgcaaa gtccgtatac atgagccacg tttccgtaaa
     5941 ccactccgtt agaaattcca agcctttgat ttttgcgaaa acaccgggaa cgaaggccgt
     6001 tcccatgata taaagaaaag aaagaagcaa actgacactg gtacttctag tgatgaatgt
     6061 caaagccact ggaacagata atgtcgctaa tatcagcaga agcaatacag gaaatacttt
     6121 tagaacataa tttccatagt ttttgattcc ctcaacttca ccaaatccga aagcaagtgt
     6181 gtaagcaatc atggctagcc ccgtaatcaa aaccacgcca agtgttgtga tcaaaagcgt
     6241 aacgagaagt ttcgacacat aataagtcat tcgattccgt cctaaagata acgtattgcg
     6301 aatcgtaccg ttttgaaatt cttccccaat ataaaagcac gcaaatgctg cgaaagccag
     6361 aagaatccat gcagccattt tttccaccat taaaatccct gttgcaccgt tgatgactgt
     6421 atccgttgct tggtttaatt cattgccgta ttttgtatgc atataccatt cgttccaaga
     6481 ataaaaaagc ggaagtacga ccatcagtcc tagtgagacc cacattttac ggctcttcca
     6541 tacttttatt ttttcagcag ttaatagatt aaacatgtgg attgcctcca gttaattgta
     6601 agaaataatc ttccagtttt tgcccaacta ggtggatacc atccacagga acttttgcct
     6661 ctaccaatgc ttgattgatg gctgctactt ggtcaattcc ttcaaaaagc tggataacac
     6721 ggctagattg gacaaaataa tccctataac caagagagtc gagaaccgtt actgcttgtt
     6781 ccgtcgccga agtgtcaatt tttataaatt gacgagattc ttgtgccaat tcttctttcg
     6841 ataattcttt gatcaagtgt ccttcatgca aaataccgta gtgagtggcg atctgtgaca
     6901 attcatctag caagtggcta gaaagcaaga cggtgatgcc ccgttctttt accaaacgtt
     6961 ggatgatttc tcgcatttct gccatccctg ctggatccat cccattcgtc ggttcatcca
     7021 aaatcaaaaa ttctggattc gtgatcagtg catttgcaat agctaaacgc tggcgcatac
     7081 ctaaagagaa attctttgct ttctttttcc ctgtattttc caatcgcatc attttcagca
     7141 ggtcttcgat ttctctatcc gacacaccgc cgttcgctgc ttggatacgt aggttatctc
     7201 ttgcagttaa ttctggataa agagcagggg tttcaatcga ttgacccata cgtcgtcttg
     7261 ctgcctgcaa atccgtcgtt ccaaataatt cgatcgaacc ttcatcaatc gagatcaaac
     7321 ccataatagt tctcataaag gtagacttac ccgctccatt ctccccaatc aaaccataga
     7381 tcattccacg tttgatttcg atcgatactt tatctaatgc tttggcagca ccgtattttt
     7441 tggttatacc agttgctttc aagaccgttt catttccaaa atccatactt atccttcttt
     7501 cttattgata agtgaagtat agttgacggg tataaagaaa gcagccagaa atgtataaag
     7561 aaagtgtaaa ttttccaatt gaatttcaag aaaaatcaga ctgtctcgca gacgtggttg
     7621 agttgtctgc caatcaaaga gcgcaccaaa cagtgctaat ccaagtggac tggccaccat
     7681 ggtaatcgtt gttatcgttg acatcagacg tccttgtata tgcgttggaa cttctcgctg
     7741 aagccagacc atataaaaag caaaagaaaa cattaatcct taaaaacgga cttatgaaaa
     7801 ataatacatg ccgtttggga tttacaatta gctaccaaat aagaggttat gacaaaagac
     7861 ttttcacaat cccttcttcc gaataaacgg tgttctcctg ccaactcctc ggtcacaagt
     7921 cacaatattt atcaaacatg agaagcaatt tttgtaaatt ccttcttatt tcttgaagct
     7981 gaccacttct gtcacaacct cagaagcgga taaataatat tgtttgatga tactttatct
     8041 ttttcccgtt tgtttataga tcgaatgata ttatagacca agaatcctac ctattaacat
     8101 agctaaaaac acaaaaaagg cactttccaa attgtggaaa gtgccagaaa agttaggatt
     8161 aaggtaactg cgctcagcat tgcttcgccc agtacgattc tcgtaactcg tgaactcgaa
     8221 cgataatcgc aattaacgtt ttgagaactg tgaagcttta cgggctttct taagacctgg
     8281 ttttttcttc gtatcattga ataagtttga aaaaccttga tttataaagg ttttcccgta
     8341 aatatatttt attatatttg attaaatttg aaatgtttgt gttctaaaat gtgttctaat
     8401 ttaaaggcag tatatttcgt attttagatt aaatttttag gctcattcat tctcaaatcc
     8461 attctattca gtcaataaaa attttaaaaa gactttataa acaaagatgt tcaagcgagc
     8521 atcttatttt tttgtgctta atttgaaagg agaacatttc atgtataaaa aacaaaaaaa
     8581 gaaattatgt gtattaacta ttttgagttt ggctgttcaa gcaattgttg gagcaattgc
     8641 tcctacgatt acttttgctg atgaaattac ccatccacaa acggtaaccg tagaactaga
     8701 tttagcacat caatatgctg ttgaaggaac ttttagtgat ggtcgtccaa tgtcagaagt
     8761 cactgttccg cattatgcag tgtacaacgg ctttaaacaa gatattttct gtattgaacc
     8821 aggagtcccg atttacaatg agttcactcc tggctatgag aaaaacccgt taccagatat
     8881 gtccgataaa gccaaattag tttccgtttt atggaaaaaa gtaggtacag atgcagatac
     8941 tcatattgtg gcacagaaga tgatctggca agaagttaat gggtataccc ttcactcaat
     9001 aaaacgatca gatggtagtg ccgtaaatat cgcagccatt gaggcaaaaa taaatcaagc
     9061 cattgacgat tatcagaaga aaccaacctt ccataatagt acggctaaaa cagtattagg
     9121 gcaatcgact actgtgacgg atacgaataa tctgaatttg tcagagttcg atgaggttgt
     9181 ggaaaatgcc gcaaacattg attatcgtgt gaatggtaat cagttgataa tcaccccaaa
     9241 tgccaattca aaagaaagtg gtgtgctgac gcttaagaaa tctgctggta ccggaacacc
     9301 tgtcgcctat aaaaaggctg gccaacagac tttaatggcc ggggcgattg ataaacctaa
     9361 tacctacaca atcaaaattg atgtggaaac ggagggttct ttaaagatca aaaaagtcga
     9421 taaagaatcg ggtgatgttg taccaggaac ggttttccat ttagactttg gaaaaacttt
     9481 acctgcaaaa gacgtgacta ctgacaaaga aggcattgct acattggatg agattcctca
     9541 tggtacaaaa gtaaccatta ccgaaaaatc agtaccggcg ccttatacga ttgataccac
     9601 ccccatgact accaccatta aagcaggcga aaccatttac gtgacttcaa aaaacgcacg
     9661 ggaaaaaggg caaatcattc tggataaaag cggagttgaa acagggagtg atctttggaa
     9721 tgacaattat tccttagctg gtaatacgtt tgccattcgc aaagacagtc caaccggtga
     9781 aatcgttcaa gaaatgacca cggatgaaaa cggtcatgcg gaaacaccaa aagagattgc
     9841 taatgcgttg gaattgggaa cctattatgt gaccgaaact aaagctagtc atgggttcgt
     9901 gaatacgttc aaacctgtaa aagtcgaatt gaagtatgcc aatcaaaccg tagcccttgt
     9961 taccagtaac gtaaaagggc aaaatcaaga agtcactggc gaaaccactt tgacgaaaga
    10021 agacaaagat actggggata aagcacaagg taaggcagtc tttgagggtg ctgaatacac
    10081 cttgtttact gctaaggatg gaaaagctgt taaatggagt gaggctttta aaccagagtt
    10141 agcgaaggga acgaaaggtt ctgatgaaac ggtcactttg actttagacg aaaagaacca
    10201 agttgccgtt aaacatttag ctatcaacga gtattattgg caagaaacaa aagcgcccga
    10261 aggatatagc cttgatgaaa caaagtatcc tgtatccatc aaaaaggtgg atgatagcga
    10321 aaaaaatgcc gtgattaccc gaaatgttac ggcaaaagaa caagtgattc gctttggctt
    10381 tgatttcttt aaatttgctg gatcagctgc gggcactacc gaaactggct tcaatgatct
    10441 gacctttaaa gtgtcgccat tggaagggac caatgaaatc acaggtgccg aagatgaagc
    10501 gaccacagct tataacgagc aattagggtt tgatggttat ggcaagtttg aaaatcttcc
    10561 ttatggggat tatttacttg aagaagtaga agccccagaa ggttttcaaa agattacgcc
    10621 attagaaatc cgttctactt ttaaggaaaa caaagaagac tttgtgaaga gtgagtatgt
    10681 ctttaccatt accgaacaag accaaaaaca accaattaaa acggtgaccg ttccttacga
    10741 gaagctgacg aacaaagcat tttctgttag tttgaaccgt ttaatgctct atgatttgcc
    10801 tgaggaagaa gatagtttaa cttctcttgc gacttggaaa gacggaaaca aagaattaac
    10861 ttctctggat tctaccgagc tagttgataa attgagttac aatctacatg aaatcaaaga
    10921 agactggtat gtggtagcgc aagccattga tgtggatgcg acaaaagctg cccaagaaaa
    10981 agatgaaaaa gccaaaccag tggtgattgc agaaacaagc gcaacgttgg cgaacaaaga
    11041 gaaaacagga acttggaaaa ttcagcataa attaaccgcc gaacaagttt taaataaaac
    11101 tattgtctta ttcaactatg tgtatgaaaa taaggaagct tttgaagcag gcgataagcc
    11161 agtagcgaag gatgttagct tgaataacca agcccaaact gtcagttgta cagtggaaca
    11221 ccatgtttct atccaaacaa aggcccacct agaaaacggt tctcagacct ttactcatgg
    11281 tgatgtggtg gatatgtttg atgatgtgtc gattactcat gatgtactgg atggctcaaa
    11341 agaagctttc gaaacaattc tgtatgcttt attaccggat ggtacaaaca aagaaatttg
    11401 gaaatctggc aagattgatt atgaagtaaa tgataaagaa ttcaccaaaa ctgtgcttgc
    11461 gaaaaaagta gataccggaa aatatccaga aggaaccaag tttacttttg cggaaatcaa
    11521 ttacgataaa gatgggaata tcaatggaaa acacaatgaa gatttgaaag aaaaatctca
    11581 aaccttaaca ccaaaagaag ttccaaccat actaagtacg ccgaaacaac cggaaacacc
    11641 aactgttcca agtggctctc aagaatctag tcccacagtg aagaccttcc ctcaaaccgg
    11701 ggagaaaaat tccaatgttt tactgttcat tggttttact ttgatttttg cgacggcggg
    11761 ctattatttc tggaatcgcc ggaactaagg tgatgagatg atacaagtaa atcctaagaa
    11821 ccaacgtgcg cgaccgcctc ctaaaaaagt agtcgcgcta tttttgtatc caaaactaac
    11881 aatgaaaaga ggaaaagaag atggaattga aatttgtcgt gccagatatg gctgaaacct
    11941 ttggaaagtt aagttatgcc ggtgaaggag aaattataac agaaggatat ggacggaata
    12001 ctacggtgat tggtcgcagt tatcatttgt attccagtaa gcaacgggcc gatgatattg
    12061 aggtggtagt agctgcggaa gccggtgaaa aggattttga tcaagaccaa ccgctaaaag
    12121 ccgtgaatcc ccatttggtt gccaaaggct atgagattga gaatcgtggg tttactgatt
    12181 acgtgttgta tgttgatgat ttagtgaaag cataggagga aaagaagatg agattaacag
    12241 aaggtattgt cgtagattca ggtttaacat ttgggaagtt gcgtttttcc gcattacgcc
    12301 gagaagtacg gaagcaaaat gaggatggaa caattggtaa tgaggtaaaa gaacgaacct
    12361 ataatttaaa atcctctgca caagggcgaa tgattcaagt tagtatccca gctaatgtac
    12421 ccttacgcga gtttgcttat gatgcagagg tagagttggt caatccaatc gtggataccg
    12481 ttgccaatta tgtttttcga gaaggaacta ccgttaattg gttcattaag gcggatgatt
    12541 tagtcttgaa gcggcagccg aatcaaggaa atcctacgaa ccaaaatgaa agaaagaaat
    12601 aggtgaagat tatggaaatt aatgttgaac atattttaga ttgtctcgat caatatggaa
    12661 aaggcgagct gactgaggaa caactcgtta aagcattaac ctctgatgaa aaaatgtttc
    12721 tgattataca tcaaggttta cttggagaaa atagcgacag tgaagaagac tctgatgtac
    12781 tagactggtt aggagaagaa gagtctttct ttatagttgt cgaagtaaat gaaaacttat
    12841 gtcggcaggc agaatccatt ttggaagaaa ttggtgtgga gatgccggat gctattgaag
    12901 gctttttaaa tcaattggtt gaaacgaaac aactcccctc ctcgtagcag taaatgacta
    12961 gcataacttt ttgaaattgc ctaatgaaat cctattgggc tttttcgatt ggaggtggaa
    13021 accatcaaaa tgtacaaagg acatcgcata cgcgcaggag atcagcactt ggtttatcac
    13081 tttgttcttg gctggctact cgctctgttc atcggttgga tgagcgtctt ttattttcaa
    13141 gagtttagac aatttgatat ttctaagcta tcactttcta ctattgaaat cgtttggtct
    13201 ataaaagatt tagtatgtct gcttggaagt ctcgcttttt caggagctat gatattgctt
    13261 tatattcact tttttcttga tcattggcga agcttgtggc atcgacaaaa gctagctcgg
    13321 atgattctag agaaccattg gtatgaagtg aaacaaactc aaagtgaagg ctttttcaaa
    13381 gatttgaata gtagccgaac cagagagact atcagttact tccccaaaat ctattatcga
    13441 atgaaagatg gattactgtc tattcgcgtt caaatttcac ttgggaaata ccaagatcag
    13501 cttttaaaat tggaaaagaa gttagaaagc ggactgtatt gtgagttagt agaaaaggaa
    13561 ctcaaagact cctatgtgga gtatacttta ctgtatgata tgatcgccaa tcgaattggg
    13621 atagacgaag tagtggcaga aaatgggact ctgcgattga tgaaaaatca agtatgggct
    13681 tatgattctt taccccatat gttaatcgct ggtggcacgg gtggagggaa gacctatttc
    13741 ctcctcacca ttatcgaagc actattgaag tcagatgcag aattgtttat cctcgatccc
    13801 aaaaatgcag atttagctga tttaggtacg gtgatgcctc atgtttattc acaaaaggaa
    13861 gaaatttctg cttgtgtgga agatttctat gaacgtatga tggctcgtag caaggcaatg
    13921 aaagaaatgt ctaactacaa aacaggagag aattatgcct atcttggact tccacccaac
    13981 tttttaatct ttgatgaata cgtggcttat atggagatgt taacgacgaa agaaagtgcg
    14041 gtgattttga ataagctcaa acaaatcgtg atgttgggtc gtcaatctgg tttctttctg
    14101 attttggctt gccaaaggcc ggatgcgaaa tatttaggag acggaatccg cgatcaatgt
    14161 gcgacatgtt tctaactgaa aaggttaggc acttaaaaga agaaaacata agtttccttt
    14221 taaggagaac tgatattttg agcagtggaa tgatggggta acgccctgaa acgctccctt
    14281 aatactccga ctggctgttt aacatatagc tgttagatgg tcagaagctc ggtgaagtcg
    14341 gctgaaagtt accgtaaata tgataaattc atatacgaaa gttgttcaga ggtgaatgat
    14401 gtaacctata ggtcggaggt ctataaaata ctcacggtga gaatgtaagt aatactactg
    14461 acgaacttgc gaatgaaaga atctatacac ggactgtata gaaatgtaca ggattctacc
    14521 gtatcaacgg aacagaatgt atgttgtggt ttagtaagat tattggttat gaacaaccat
    14581 gcaatgttac aggcattgga aatatacagg ttcaaagcaa gcacctaaaa gtatatataa
    14641 agataaaaat atcgaaacat gggaagctgg caatgtggag gaaactatat tccattgaaa
    14701 catttgtgac gaagaataat aagcacattt atgccagtga aagtggtggc acagtaccaa
    14761 tgaggatata gaaatatatc ggaggaatag ccactagtca aaagaaatcg ttaattaaat
    14821 gatgtatttc atagaatcga gtaagacaaa ataaacacta caacctaagt aaacaaaggt
    14881 agtggtgcta tgaataaagt aaaaacaaat aaaacacttc gtcatagtga atactatggc
    14941 atgacacaaa tatttgatga actgtatgta aaaagtgata aaaaagaagt ttttacagag
    15001 ctcatgacaa ttattatgag tgatgaaaat attcgactag catacagaaa tatcaagaat
    15061 aacaagggca gttatacagc gagtaccgat aagaaaagta ttcgagacat taaaacactg
    15121 tcagaagata cctttctaac tgaggttaaa gttctttttc gctgttacaa accacgagca
    15181 gttagacgtg tagaaattcc caaaccaaat ggaaaaacac gccctctggg cattccatca
    15241 atctgggata ggttaataca acagtgtatt ttacaagtct tagaaccgat atgcgaagct
    15301 aaatttcaca attatagtta tggatttcga ccaaaccgtt ccacacatca tgcgattgct
    15361 acctgcaatc gactggcaaa tagggggaag ctacactttg tagtagatgt tgatattaaa
    15421 ggattctttg ataatgtcaa tcactctaaa cttttgcgtc aaatgtggac gattggaatc
    15481 cgtgataaat ggctattagg tattattcga tccatgctaa atgctcctat agtcctacca
    15541 accggtaaag tcgaacaccc taaaaaaggt gtacctcaag gtggtgtcct atccccttta
    15601 ctatctaata tcatattgaa tgaattagat tggtgggttg cttcacaatg gcaaaatacc
    15661 acgattgaga cagaaaagat tatggatagg cgagataaag gcaaaggcat tgataagact
    15721 aatcagtatc gggcattaag acgaacaaac ttaaaagaaa tatacattgt tcggtatgct
    15781 gacgacttca aaatcttttg tagaaaacga agtcatgcag ataaaatatt tcacgcaacg
    15841 aaaatgtggc ttaaagaacg gttatcactt gaaattagcg aagaaaaatc caaaattgtc
    15901 aatctcaaaa aaggtaaatc agaatatcta ggatttgaat taaagctaat caagaaaggt
    15961 aaaaaatacg tagtcgaaag tcatatgagc aaaaaatcaa tgacaagagt taaaatccaa
    16021 ttaaagaaac aattacgtaa agttgccaga ccgaagaatc attgcgaaca agcaaaagaa
    16081 attggattat ataattctat ggtgattgga atccatcaat attatggaat tgcaacctgt
    16141 gtaaatctcg attgcagtaa aatagcctat acactaaaac cgttcatcaa tcacaaactg
    16201 ccgacaaaga aacatggaaa gatattaaat acatttatca aatcgaagta tggaaaaagt
    16261 aaagaaatgc gatggttgaa taatgtgcca attgtaccgc tcaactatgt tcagtttaga
    16321 cttgctgtcc ccctatcaga gggaacttgc aagtacactg aaagtggacg aagcaagata
    16381 cacagtaaat taaaattaga cctagccctt ttacttcaca tgatgagaaa tagtaattat
    16441 gaaagaagta ttgaatttgt ggataacaga atatctaaat attctgctca aaagggaaaa
    16501 tgtgctatca cgggtaaatt tctcgaatac gaagaaatcc attgccacca tatcgtacca
    16561 gtaaaacaac agggtacaga caaatacagc aacctcatta ttattcacaa aaatgttcac
    16621 gcattagtcc atgctatcga agaaaaaatc atacacaaat acctgaatgt attaaatcta
    16681 accaatgaac aaatagaaaa actaaatgaa ttacgtgtca aagctggtaa tagtgtttta
    16741 acagtttaac ttgaaaaata tatcatactt aacattcttt tgatggaacg ccgtatgaag
    16801 tgaaagtttc atgtacggtg tgaagcgggg gaaaatctgg agatgacatc aaaggattac
    16861 ctatcgctat tcaactttcg cgtggctttg ggtcgtatga gtgaactcgg atattccatg
    16921 atgtttgggg aggtcgaaaa aaacttcttt atgaagcaca tcaaaggtcg aggttatgtg
    16981 gatacaggtg gcagtgtgat tagtgagttt tatacgccac ttgtaccaaa agggtatgat
    17041 ttcttagatt caattagtaa attagaaaag atgaatgatt gagtgatctt caatactgac
    17101 tttatacttc aagtaacgta taatactttt atatagtagg aagaaagggg ataattaata
    17161 tggaaagtaa tgattcttat ttagagtatg caaaagcaaa taaggataag tttatttctt
    17221 atattatcaa agggaaatcc cccgaagttg gacgagacgc agtttttatg gcagggagtc
    17281 ctggggctgg aaaatcagag gttgccattg gattagctga gaattatgat aaccatgtga
    17341 ttattgatgc ggattacttt cgcacccagt ttcctgatta taatggaaaa aactctagtc
    17401 tctttcaaaa agcatcttct tggttggtag agcaggcatt tagatatgtc ttagatcatg
    17461 gctattcttt tattctggat gcaacttttg caatattaag tgctgaaaaa aatatctcgc
    17521 gggcgttgaa aaaacatttt cgagtgacga ttttctatgt ttatcaagac cctaaaatcg
    17581 cttggaaatt tacacaagaa cgtgaaattg cagagggacg tcatgtgccg aaaaaggcat
    17641 ttattaacgc ttttttcaaa tcaagagaga acattgcaaa agtaaagagt aaacatccag
    17701 atgtattgct tcatatcata ataaaagatt atacgaataa tatttccgaa gttcattatg
    17761 ctacggataa tctagaatta tttttaccac tagggtatag taggaaagat ttggaggttc
    17821 aattacatga ctgagaaaga acaagtaacg aaaattgtaa agaaatacaa taagagcatt
    17881 gcggatttat cggaaaatgc tactgcaaaa gagttcaaaa cggtgattaa atatgtagcc
    17941 gatcaggcca atgaaaaaca acggaaatta gttggattaa ataaaaaata gtaggacaaa
    18001 ttaataatgt atgagccaag agcaaaatgt tcttggcttt tttctttgca atgaatgagg
    18061 taatgtatga gatttttaga tttattcgcc ggtattggtg gctttcgttt aggtatggaa
    18121 caggcgagcc atcattgtat cggtttttgt gaaattgata aatttgctag acgtagttat
    18181 aaagcaattc atgatacaag taaggaggtg gaaatgcatg acatcacaag tgtatcagat
    18241 gagtttattc aatccctcgg accagtggac atcctttgcg gcggatttcc gtgccaagct
    18301 ttttcaattg cgggaaagcg gcaaagattt tccgatactc gaggtacttt attctttgaa
    18361 atcgctcggt tcaccactct tctccgacct aagcttttat tccttgagaa cgtccgagga
    18421 ttgctcaatc acgaaggagg ggctacgttc gagacgatcc tccgaacgct ggatggattg
    18481 gggtatgacg tggagtggta ggtgcttaac tcaaaggcct acattcccca aagccgggag
    18541 cggatattcc ttatcggaca ttctcgagac gcatgtaccg aacaagtatt tcctatcatt
    18601 ggaccgtctt caacatctga tcaaaacatc agaaatttgg taaatatcaa tccttccaat
    18661 cgaggaatgg gtggacaagt ctatggttca gatggcgtag cgccaacctt aacaggtgat
    18721 gaaggaatca aaatcgcact gccagcaagg gatggaatct ctgtagcagg actattaccg
    18781 ggaaagtttg aacaaggtaa tcgagtttat gaagttactg gaaccgcccc caccttgtca
    18841 acgaaacaag gtggtacaaa aatcatgatt cgaaaaaatg aaacagaata tcaagaggtg
    18901 aagccagaag acagtgtgaa tctggctttt cctaattcca caagtcgccg aggtcgtctg
    18961 ggaaaacaga gtgttcatac cttactaaca ggagaccaac aagcgatcgt cacggatcag
    19021 tatcaaattc ggaagctaac acctagagag tgttggcgac ttcagggctt tcccgattgg
    19081 gcttttgatc gagattctca agtcaattca gatagtcagt tgtataagca agccggaaac
    19141 tctgtaacag ttccggtcat ttttgatatt gccagaagat taaaagagtt ggatgtacat
    19201 ggtttatgat gagttgattg gagaaattta ttgggtgatt ggaaagattc agtcagaccc
    19261 agaattagaa gaacaattgc gacggttaaa atttgatatt cgcaagaatg gtgtcaaagt
    19321 cgctggcgat ccatatttaa tgaatgagga gacggatgct cgaattgaaa taaatcaagt
    19381 aattgcagaa tttgagcgga ttgcggacca agcaaaagaa ccagatattc gtcaatactt
    19441 gttagaaatg aaagcagagt tggaagtaaa tggtattaca gatgagtaac ctaaaccaac
    19501 acaactgact gttaggcgct gaccgcggag gattcgcgaa gcagaaatcc tccgtggtgc
    19561 tgtgcctgtt catggacggc gttagccgac catgatgtct cacccccgta tctaacaggg
    19621 gggtacaaaa acacaaaaaa taagtaagca accattaaga atgcttgttg tatcaaagat
    19681 agtaagtaat ttgtataggt gttaactcat ttgaaaaagt taacaccttg aatttggagg
    19741 tgagaacttg gcacaacgga atttagatta tcggctatta aaagaccgac gcaacgaata
    19801 cggcgtttca caaaataaat tggctacggc ttgtggactt agtcggccgt acttaaatca
    19861 gatagaaaat ggtggcgtaa ccgcatctat aaaaaccatg aggaaaattt ttgaccaact
    19921 ggaaagcttt aatcccgatt tgcctttgac gttactgttt gactatgtaa ggattcgctt
    19981 tcccacaacg gatgcacgaa aaattattca agaaattctc catttgaaat ttgagtatat
    20041 gctccatgaa gattatgcct tttactccta tcaagaacag tatgtcatgg gtgatattgt
    20101 ggtgatgttg tctcatgaag aagataaagg tgtgctttta gaattaaagg ggcgaggttg
    20161 ccgacaattt gaaacttttt tactcgctca aaagcgtagc tggtacgact ttttcgagga
    20221 ttgtctaaag gccggtggcg tgatgaaacg cttggacttg gcaatcaatg atcgagtggg
    20281 gctattggat atacctgagc taacgaaaaa atgtcagaag gaagaatgta tctccttgtt
    20341 tcgtaccttt aaaagttatc gttccgggga acttttaaag gctgatgaaa aggacggcat
    20401 gggcaatacc ttatatatcg gcagtctcaa gagtgaggtt tatttctgtt tgtatgaaaa
    20461 agactatgaa caatatatca agttagggat tccattagac caaactgaga cgaaaaatcg
    20521 ttttgagatt cgactgaaaa atgaccgtgc ttttcatgcg attcaagatt tgttaaaagg
    20581 tcgtagcatt gagagcacca cgttttccat tatcaatcgt tacttgcgat ttgcggataa
    20641 agtagaaggt aaaaggcgaa ccaactggcc cttgaatgaa caatgggggc gttttattgg
    20701 acggaatcgg aaagagattc aattaacttc cgagccaaaa ccgtatacca ttgaacgaac
    20761 cttgaattgg ctaggccgac aagtcgcccc tacgtggaaa atggctaagg agttggatcg
    20821 attgaatcgg acaacctata tccaggatat ggtacagaat gcacgcttat cagaccgaca
    20881 taaaaagatt ttggagcaac aaagtatggc aattgaaaat ttgattatat gaaaagagga
    20941 atcgtcatgg gaattgtaaa agatattttt ttggtatttg gtggcagtat gcttggtgtt
    21001 actgtgatgt gcttgatgaa tgcaagtgca gcagcggatc gagcaatgga aaagaaagga
    21061 aagtgacaag atgaatttcg gacaaaattt
//
LOCUS       DACEUZ010000036        20903 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.036, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000036 DACEUZ010000000
VERSION     DACEUZ010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20903
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.036"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(93..536)
                     /locus_tag="IXG44_001165"
     CDS             complement(93..536)
                     /locus_tag="IXG44_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HAQ1657897.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(754..2094)
                     /locus_tag="IXG44_001166"
     CDS             complement(754..2094)
                     /locus_tag="IXG44_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1657898.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            2363..3259
                     /locus_tag="IXG44_001167"
     CDS             2363..3259
                     /locus_tag="IXG44_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAQ1657899.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(3409..4350)
                     /locus_tag="IXG44_001168"
     CDS             complement(3409..4350)
                     /locus_tag="IXG44_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAQ1657900.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            4476..5597
                     /locus_tag="IXG44_001169"
     CDS             4476..5597
                     /locus_tag="IXG44_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAQ1657901.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRKNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            5855..7357
                     /locus_tag="IXG44_001170"
     CDS             5855..7357
                     /locus_tag="IXG44_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAQ1657902.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            7424..7951
                     /locus_tag="IXG44_001171"
     CDS             7424..7951
                     /locus_tag="IXG44_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ1657903.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            8162..9223
                     /locus_tag="IXG44_001172"
     CDS             8162..9223
                     /locus_tag="IXG44_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAQ1657904.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            9226..10122
                     /gene="murQ"
                     /locus_tag="IXG44_001173"
     CDS             9226..10122
                     /gene="murQ"
                     /locus_tag="IXG44_001173"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAQ1657905.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            10136..12118
                     /locus_tag="IXG44_001174"
     CDS             10136..12118
                     /locus_tag="IXG44_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1657906.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(12183..12629)
                     /gene="nrdI"
                     /locus_tag="IXG44_001175"
     CDS             complement(12183..12629)
                     /gene="nrdI"
                     /locus_tag="IXG44_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAQ1657907.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            12840..14264
                     /locus_tag="IXG44_001176"
     CDS             12840..14264
                     /locus_tag="IXG44_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1657908.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(14445..15218)
                     /locus_tag="IXG44_001177"
     CDS             complement(14445..15218)
                     /locus_tag="IXG44_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ1657909.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(15215..16120)
                     /locus_tag="IXG44_001178"
     CDS             complement(15215..16120)
                     /locus_tag="IXG44_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1657910.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSYKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSIKGEEEK"
     gene            complement(16274..16594)
                     /locus_tag="IXG44_001179"
     CDS             complement(16274..16594)
                     /locus_tag="IXG44_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HAQ1657911.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            17068..17754
                     /locus_tag="IXG44_001180"
     CDS             17068..17754
                     /locus_tag="IXG44_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HAQ1657912.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            17756..18700
                     /locus_tag="IXG44_001181"
     CDS             17756..18700
                     /locus_tag="IXG44_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HAQ1657913.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            18899..19543
                     /gene="pcp"
                     /locus_tag="IXG44_001182"
     CDS             18899..19543
                     /gene="pcp"
                     /locus_tag="IXG44_001182"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HAQ1657914.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            19622..20899
                     /locus_tag="IXG44_001183"
     CDS             19622..20899
                     /locus_tag="IXG44_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1657915.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
BASE COUNT         6754 a         3766 c         4343 g         6040 t
ORIGIN      
        1 cttattcacg gcgttccaga agtagcttct tcttatttct tgaagctgat aacttctgga
       61 acatcctctt tttttatttg gacggataat ggtcattcca tttctttgat catttttttc
      121 attttgtcga tctcttcttc ttgtgcctga atgatttctt ccgctaattt ttttacttca
      181 ggatctttta aatcagctcg tttacttgcc aggatagcga tagaatgatg aggaatcatg
      241 gccttcatcc aagctgtgtc gttgacagta gtttggcttc gtaccataaa caaggaaccg
      301 gcaaaaacaa gaagactagc ggctaagatc ataacattta tctttttgtt ttcatacatt
      361 ttccacatga acaacagcat gatgactgcc atgactgcac ccatcatcaa cgccatgaat
      421 actcgtgtac gactgaagaa gatatgatcg agcgcataga cattcaagta catcatacca
      481 aacataacta ccgttgaaac accgatcatc aacaaaaact tccaatactt tttcatttaa
      541 aacaaactcc tttttttcat tcctttcttc tatcatgtca tgagcatgga gaacaatcaa
      601 ttattttgca cagagggttt ttgaacaccg tttaccaaga ggtcatgaga ctgtttattc
      661 ggaaaaagga gttgcaacaa gacttttgtc acaactcctt tcttcaatag ctccatctat
      721 tccacataaa taaaataaaa ttttctatcg atttcattca ttcgtcaata cgaacgcatc
      781 attctttttt tgataactga aataaatgaa caggtaaagc aggccagtca atacagttac
      841 cccaatggag ttcgtgaaca ggataccaag gaaaatcaat cctaagacag ctaatgcaaa
      901 aaagctgatt cctttatccc ataatgttcc ttttcttagt gccagtacaa aggatgccgc
      961 actgataatg atccagatca ataatactgt atacgaaagg gaacctgcca gatagccaaa
     1021 taatttgtca ccgacaaaat aagaaagagc tacaccaaga taaagaacac tggtacaaaa
     1081 taaaacagca cgttgcggta cttgatgctt gtttaaaaca gctagttttc tagaggaacc
     1141 ttttcgatcc ttcaaacgga agaacaaaat tcttgaagat gcatacacac ccgaattgat
     1201 agatgagaac aaagcaagga caatcacaaa attgacgata tccgctgcaa atgggatatt
     1261 cattttttca aataccatca caaaaggact gacattcgtg cctgctaaag tattccaagg
     1321 aaaaagaatc aaaagcaaaa acaaaggaat gatgtaaaag gaaatgattc ttcccatgac
     1381 acctttgatt gctttaggaa tcgcacgttt aggatcatct gcttcgctta ccgtgatagc
     1441 aatcagttca gaaccgccgt aagaatagat aactacaagc aaagaactga tgattccctt
     1501 cataccatga ggagtaaacc caccatggtc agtcaattgc tgaagggtag ggaaaacgcc
     1561 tgtgcccaac atttgctgac cgacaagata agtagcaaaa atgatcagta agataatgac
     1621 actgatttta gcaaaagcca gccaatattc tgtttctgcg aataaccgta ccgaataaag
     1681 attgatgatc gtagttaaaa cagccaacat aaaaacaaat acccaggctg gaatgtgcgg
     1741 gaaccacaac tgcaaaaagg aagcagctgc aacagcttca gcgatgatat tgataatcca
     1801 catagaccag tagacccaat cagtaaaatc agcagaatga gcacctagat aagggcgtat
     1861 caaaccagat aatccgtgat gcgtgtcgcc accgcttaaa acgatttttc ccagaccttt
     1921 catcacgata tataaaatca gcccaccaat caaaaaagca atcaatacag aagggccggc
     1981 gatcccaatc gcttcgccac ttcctttaaa tagacctgca ccgattgctc cacctaaagc
     2041 taacatggtg atatgtctgg agctaagttg ttttttcaat tttttatctt ccataaatga
     2101 cctctttctt ttaaattcac aatcaaaatt tatattaaga ataaatagca atatttaaca
     2161 atgactaatg ataacaaatt ttcagacaat tacaactatt cagaaaactt ttaggcgaat
     2221 tttaatagaa gaatgattta tttcatcata ttttcataca aatgttaaat tttcatacaa
     2281 atataggaaa cgttttactg gctttttact atttaaacag gctatcatag gaaaaagtga
     2341 cagagaaagt gaggcggaaa agatgaacaa aaatgacgta ctgctagttg caaaaaataa
     2401 atcactttat tttttgcctt ccatccgctc tttagatgtt tggtccggaa aagatttact
     2461 acagtcggaa ttcattaaat cagctagaca acagttggca aattatcagc tgatcgtttt
     2521 tttagattat ggatttgata tggagatggc tcgacgcgtg cgcccattta ccaatgcaaa
     2581 aattgtgtta tttttttgga atcacttcaa agcagaacac gaggcgatgc tgaagcaggc
     2641 acaagatgaa ccggcaatcg atgagattta tcattttgat tttttggaag caaaagaact
     2701 tgggctgaaa cataatagtt cattttattc gaaacatatg aatttttctt ctagcgaacc
     2761 aaagacggat ctattttttg gtgcaacgaa taatgggaga aaagaacgag cagaaagcta
     2821 taagcaggaa tttataaaaa gagatgttac gaccaactat ttcattcttc cacatcgagg
     2881 gaatgaacaa cctggatatc ttacttatga agagtacctg aaaaaaacag gggaatcgcg
     2941 cgggattctg gaactgttgc gggagggtca gcaaggagtg actttgcgga catttgaatc
     3001 catgtatttt caaaagaaac tagtgacgga taatcaagcg attgctcatt atcattttta
     3061 tcgtcctgaa aatatttttt tgcttcgaga acgtgattta gatgaattac cggaatttct
     3121 gcatacacca tttaagccaa tcctatcaga gctattggaa ttttatgatg cagaaaattg
     3181 ggcaaaacga tttatcacta acgattccac tatttatgag caatacgaat atgacctctc
     3241 ctctgttgaa aacaaataaa aaacctacat tttgaagaga ttatgtactg aaacaaacaa
     3301 gcacaattct tatggcatca tgagagttgt gttttctttt tgatctttta ttgggcttaa
     3361 aaaaagagaa ccagctgctg attcagctga ttctcatcag agagcctttt attctgcgcc
     3421 aaggattcct tcttttgcat ggactaattg ggtcaaaaag gcacaatatg gtgtagcaat
     3481 atcatatttt tgtccttttc gccaaatggc accattgatg tagtcgattt cagtcaaacg
     3541 atggtttttg atcaggtctt ggtacataga tgggtaatgc aacccgatgt tgtcaggatc
     3601 ataagtagaa gcaatatgtt ggtacacttc ttcttgatct agtatgattc cttcttttgc
     3661 tgcgatagaa gcaaattcag atacgattgt tcgaaccatg ctctctgccg ctttttgcgc
     3721 gcctagttca gcaatattac aatccaaaat cgtgcataaa ccattcaatg ttccatttac
     3781 acaagctttt cgccaaatgg aataacgaac gttatggcta tagatcggat ttaaatttgc
     3841 ttcttgaaag acttctacaa ctttttttgt aaatgcttcg ccttctggtt ccaagttctc
     3901 tagttcgatt tctccatcac ctaacaatgt gacttttccg ggaccagcta gaccagctgt
     3961 ccacatcgtg atcccaaata agatattttt ctttggcaca tatttttcca gtacatcttc
     4021 atgacctaat ccatttagta aacacaaaac ataagtattg tcagtaataa tggattggat
     4081 cgaacaaaac atatcatcta attgattggc ttttgttaat gctacgatca agtccacgtg
     4141 ttcgttgctt tcgatgattt cttctggtga atagattggc agttttgcga ccacttcttt
     4201 accgttaaag tcagcaatca acccatttgt acgaatggct tcaatgtgag caggccaacg
     4261 atcgatcaag aggacctcat ttccgctttg atgtagcatc aatccgattc ggctgcccat
     4321 tgctccggcc ccagctattg cgattttcat gttttttctc tcctttattc aatgagttcc
     4381 cttttagtat atagaaaaat ggaagaaaat agcattgttt tgtttgaaaa aaatgaaaaa
     4441 aagtgtctta cttaagaaga aagtgaggga tacagatgga tattttggcg gcaattgatt
     4501 catttaaagg aagtgcttct tcatttgaac taaatcaggc agttctaaat ggctgggaag
     4561 atcgtaaagg aagaaaaatc aatgtgccga ttgcagatgg cggagaaaat actgcagaag
     4621 caatctatta cgcgctcagg ggaacatggc gtaccattga tgggatggat ctcttattcc
     4681 gtcagcgaaa aatcagctat ttattgacaa cttggcaagg aaaaaacacc gcagtcatcg
     4741 aatctgcaca agcattgggg ttagatctac ttgataaacc aacggatcaa actatccgac
     4801 ttgcttccag ttatgcctta ggagaactta ttaaagatgc actgcttcaa gaagtagaac
     4861 aaattattgt cactttagga ggaagcgggt gtagcgatgg tggtctagga ttactccaat
     4921 ccttgggtgc ttctttagaa gggtacacag aaggaaatcc tttattatct actaaaaaac
     4981 ttcattttga tcgaaaaaac gttcttgttt ctaaagtaaa attgattatc gctgcagatg
     5041 ttaccaatcc atatacaggt caacaaggag ctgcttggca atttggaaga caaaaaggag
     5101 gaatagatac gacgctagct ttcttagata aacaagcagg aaagattgct cagcaaataa
     5161 aagatttctg ggaggtggat ttagatgata ttccaggaag tggagcagca ggcggtattg
     5221 gtggagccct ttttttgttg ggagcgaaga tggtttctgg tttcgattta gtcagccaaa
     5281 tcactggatt ggaaaaaaag atcgaacaag cagatgtcat cattacagga gaagggcgga
     5341 tcgatcagca aacgatccac ggaaaagttc catacggtgt agcagtgctt gcaaaaaaat
     5401 atcgaaaacc aataatcgct atttgcggaa gtagagacca agagctagaa ggactttctg
     5461 attttctccc aattgtctta agtattcatt tgggtccaat aggtttagaa gaagcaatgg
     5521 aacacaggca aacattagaa aaaacaaaaa tcttaggaca gacacttagc agatttttca
     5581 ccatcctccc gaagtagttg acaaaatcat tttctttatg ctatcgtaat ttcaaatata
     5641 ggacaaagtg agggatccgt gcattgaaaa attaagtcga ttgatgaaag ctgcattcag
     5701 actgtacctt gtggagacaa ggaagcagtg tgcagatttt cgggattggc ttggtttttt
     5761 cgatgtatgg gtctttttgt gtgtatccaa cccttgcatc ctctttagcc agttctgaaa
     5821 ataggactgg tcttttttta cgaggtgatg gaaaatgtct aaaatcgaaa taaaaaatct
     5881 gacattcggc tacgacagcc aaggcacatt attatttgaa caagcaaatc taaattttga
     5941 cacacaatgg aaactaggac ttatcggacg aaacggtcga ggaaagacaa ctttactgaa
     6001 tattctacaa aacaaactac cttatcaagg gcaagtaatc catcagcaag aatttgccta
     6061 tttcccgcaa cagacaaaag ataaagaacg tttaacctat tacgtgttaa atgatattac
     6121 ggattttgag atatgggaaa tcgaaagaga gctccaattg atgcaaacag atcctgaaat
     6181 cttatggaga gaattcagca cactatcggg gggagagaag acaaaagtcc tactggcact
     6241 tttatttgtg gatgacactc atttcccgtt aatcgatgaa ccaacgaatc atttggatat
     6301 ctctggtaga aaacaagtag cggcttattt gaaaaagaaa aaacaaggct tcatcgtggt
     6361 cagccatgac cggggattta tcgatgaagt agtggaccat gttttagcaa tcgaaaaaag
     6421 tcaactggaa ctttatcaag ggaatttctc tatctatgaa gaacagaaaa aacttcgtga
     6481 tgaatttgaa atggctcaaa atgaaaaatt gaaaaaagaa gtcagtaggc taaagaaaac
     6541 agcagctgaa aaagccgaat ggtctcgttc ccgagaagga gataaaacaa agaaacaagt
     6601 cggattcatc gatactgaat ctagacgagt gaataaagga gcagtgggtg ctgatgctgc
     6661 acggacgatg aaacgatcca aagcaatcgt gaatcggatg gagacccaga tcagcgagaa
     6721 agaaaaacta ttaaaagata tcgaatatat cgattctttg acgatgaata gccaagcgtc
     6781 tcaccataag cgacttttaa gcgtagaaga tcttcaatta gggtatgaaa atctgttatt
     6841 cgagccaatt cattttacaa tcgagcctca tcagcgggtg gcgatttcag gtcctaacgg
     6901 tgcaggaaag tcatccatta tccattatct tctgggggca ttcaacggca aggttatagg
     6961 agaaaaatac cagccaaaac atctgagcat tagttatgca agccaaaatt atgaagacaa
     7021 tcgaggaacg ttggcggaat ttgcagagaa aaaccaagta gactaccaag catttttgaa
     7081 caacctccga aagcttggga tggaaagaga tgtttttcat aacaagatcg agcagatgag
     7141 tatgggccaa cggaaaaaag tggaattggc taaatcttta tcacagccag ctgaactata
     7201 tacatgggat gaaccattga attatttgga tgtcttcaat caagaacaat tagaacaact
     7261 gatcttgaac gtgaaacctg ccatgttact agtggaacat gatcaaacct tcctggataa
     7321 agtatctact gagattattt ctcttgagag aatctaaccc ttgtaatctc aagaacaaca
     7381 aggtatggtt aaggggaact ttgccaacga aggagagaat cacatgcttt atgtaacgag
     7441 acacggagaa acaacttgga acgcacaagg attagtttgc ggacgggcag atgtgccgct
     7501 gactgaaaaa ggacagatgc aggcacagaa actagcagaa aaggtagtag atttgcctgt
     7561 tccaatcacg aaaatcatcc attcccctct tcaacgagca agagatacag cacaagcagt
     7621 tgctgatcgt ctcagcttgc cgctgaccgt tgatgaacga ttggtagaaa tggattttgg
     7681 ggattatgat ggactgccaa gcaaagatga gaactttcaa aaagcacgtt tagcgttcgc
     7741 tgtacgattc ccaaatgggg agtcagtctt agatgtgtat gcacggatcg ttcctttatt
     7801 gaaagaatgc atcgaagacg aagaaaatgt ctatctgtta gtttgccata atgccttgat
     7861 aagagtgatc aatgcttatt ttcatccgat gcctaatgag gggttcttta cattcatggt
     7921 agataataca gaattggttt cttatgagta attatgtttt tgtgttattt aaataaaata
     7981 tttcaaggca ctgcttttgc ttagcagtgc cttttttagg ttatgacaat tcttttgata
     8041 atataaactc aacctttaga aatgaaattt aattatttta ataaaagtat tgaaattttg
     8101 tttctttaga tatatactct agtcatagaa atgaagacgg atacagaaaa agagggataa
     8161 tatgtttggc ttttcaatat ttatgaacga ttctttgtca gaagagaaga aacagtatat
     8221 ccgccaaatg gcatccaatg gctttgtcgg tattttcact tccatgcata taccagaaga
     8281 tgatgtttcg gcatatcaaa agcgattgat cgaactagga agcgaagcga aaaagaataa
     8341 actagaattg atggtcgata tttcaggaga ggcattggaa cgagcgggtt tttcattaga
     8401 agatctaacg cctttgaaag caattggtgt aacaggttta agaatggatt atcatatttc
     8461 caatcagcag attgccgact ggtcacatca attgaaaatc agtttgaatg ctagcacgat
     8521 cacgcctaaa gatatcgatg aattgaaaga agcagaagct gatttctcac agattgaagc
     8581 ttggcataat tattatccac gtcccgaaac agggctggac aaagaatggt atcagaaaaa
     8641 aaatcaatgg ctcaaaattc aaggtctcct tgtccaaggt tttgtaccag gagatacaga
     8701 gttgagaggc ccactatatc aaggattgcc aactttggaa gaacatcgag ggatccatcc
     8761 tcttgcaggc gctctagatc tattagcaag taacacggat attgtttaca tcggagatgc
     8821 aggtctgtca gaaaacgttc aggagcaatt tgcttctttc caaaaagaac agacagtctt
     8881 gctccatacg gaacctgtag atgaagaatt ttacgaatat atattaggga agcatacgaa
     8941 tcggcaagac gatgcaagag atgtcatacg aagcgtagat gcacgttttc gagagattcc
     9001 gcctatccca gcaagaaata cagctacgcg tatgaaaggg tctattactt tagataacga
     9061 aaagtatttg cgctatatgg gtgagatcca attaaccaaa tatgatttgc cagcggatga
     9121 aaaagtgaat gtagtagcta aagttatcaa agaagagcta ccattgatca accaaatcaa
     9181 agctggtatg aactatcaat ttataagaaa agaaggaaga taaaaatgag caaaatcaat
     9241 ttagagaacc taacaacgga acgcagaaac caagaaactt ttggattaga tgagatgagt
     9301 atttctcaag ccgttcaact aatgaacaaa gaagatcaga aagtggccgc tgcggttgaa
     9361 aaagaactga caagtatcga accagtgatt gctaacacga ttgagtcctt taaaaaaggt
     9421 ggacgtctga tttatatggg agccggaaca agtggaagac taggtgtgtt agatgctgct
     9481 gaatgcgtac cgacattcgg tgtagaaccg gaaatggtca taggtctaat tgctggtgga
     9541 gaagaagcaa tgactgtagc tgtcgaaggg gccgaagatt cttatgagct agggcaagaa
     9601 gatctaatca agctgaattt gacaaaaaat gatatagtta ttggtatcgc agctagcggt
     9661 cgtacaccat atgtgatcgg tggattagat tacgcaaaat cgatcggtgc tgtcacagga
     9721 acgatttctt gcaataaaca agccgaaatc agtaaacacg cggatttccc aatcgaagtc
     9781 gatgcaggtc cagaattttt gacaggctct actcgattga agtcaggaac agcacaaaaa
     9841 cttatcttga atatgatttc tactatcagt atgattggta taggtaaagt attcaataac
     9901 ttgatggtcg atgtcaaacc aacaaatgaa aaactaatcg aacgctcaaa acgaatcatc
     9961 atgcaagcga cagaagctga ttacgaaaca gctgaaaagt actttgaaga agcagatcaa
    10021 aatgttaagc tggctatcgt catgattttg acaaatagtg ataaagagga agctcaagca
    10081 aaattagctg aagcaaacgg atttgtaaaa gggaccttat gagggggaaa agaccatggc
    10141 aaaaaacgaa ctgactttgg aagaactggc aaaacaaatt tataaagggg caggcggcat
    10201 gggcaatgta gaaagcattg tccactgcat gactcgtgtg cgtatgaaga tcatcaatga
    10261 tgatcttgta aatgtctctg aattaaaagc gattccagga gtgcttggtg tagtagaaga
    10321 tgaacagctt caagtcatca tcggacctgg gaaagtgaac aaagtagcac agcaaatggt
    10381 tgatttggca ggcgtgaaat taggagaaaa actttcttct gtaagtacag ctacagcaaa
    10441 ttcatcagca tctggaaaag accgtgtaaa cgaacgggca caagaaatga aagcggcaca
    10501 aaaatcgaaa caaaaaccat ctaaaatcaa atcggtatta aaagacattt ctaatatctt
    10561 tgtgccaatg attcctgcat ttgtcggtac tgggatcgtg gcagggattg ccgcagtttt
    10621 agcaaactta gtcacagccg gaacacttga tgcagcaaca tggcaacaat atatcgatgt
    10681 gatgaacatc ttgaaaaacg ggatgtttag ctacctagtt atctatacag gtatcaatgc
    10741 tgcacaagtt tttggtgcaa caccaactct tggtggagtt atcggggccg ttgtgatgct
    10801 gacagggatg aatccggaag caccactgaa aaacttattt actggtgaag ctcttgcagc
    10861 tggacaaggt gggatcctag gtgtcatctt cgctgtttgg ctattgtcaa tcgttgagaa
    10921 aaaactgcat aaagtagtac ctgatgcggt agatatcatc gttacaccaa ctctttcatt
    10981 gattgccatc ggtctgattg aaattttctt catcatgcct ttagcaggtt tcatttctga
    11041 tggcatggtc ggtgggatca actgggtact tggcgttggt ggagcatttg ccggcttcgt
    11101 tctaggcaca ttattcttgc cgatggttat gttcggactg catcaaattt tgactccaat
    11161 ccatgtgcaa atgattgatg aaactggtcg tacgctattg cttccaattc ttgcgatggc
    11221 tggtgcagga caagtgggtg cagcattggc attatggatt cgctgcaaaa aagataaaga
    11281 attaacagaa atgatcaaag gagctttacc agtcgggatc ctaggaatcg gcgaaccatt
    11341 gatttacgga gtaacattac ctctaggacg cccatttatt actgcttgta tcggtggagg
    11401 tatcggcggg gcagtgatcg gaatgatcgg aaatgtcggg gctatcgcta ttggaccttc
    11461 aggagccgct ttgatcccat tgatctctga cggtaaatgg ttaggctaca tccttggatt
    11521 attggcagct tatgctggtg gcttcgttgc tacattcttc ttcggtattc ctaaagaccg
    11581 tttagaaaaa gaagaattag cgactgaaac agccacagta aatgaagtgc ctgaaaaagt
    11641 agtacctcaa acttcagctg aaagtgtgac tatcctctca tcagttgctg acggaacagt
    11701 tgtttcttta gataaagcaa gtgatccagt ttttgcacaa aagatgatgg gtgaaggcta
    11761 ctttgtagaa ccagaaaacg gacatatcta ttctccagtt actggggtag tcagctctgt
    11821 cttcccaacg aaacacgcta ttgggatcac aacgccaaat ggtttagaaa tcttgcttca
    11881 tatgggaatc aacacagttg acttaggcgg tacgccattt gatgtgaaag taacagaagg
    11941 gcagcaagtg acacctgata cgcttgtagc agaagctgac ttagaagcga tcaaagcagc
    12001 aggcaaagaa acatccatga tcgtcttggt aaccaatatg gatcgtgtaa aaaactttgt
    12061 cttggaaaaa acaggtaaag caaaagcaaa agcacctgtg atggatgtca aagcttaata
    12121 tgatagaaac aatagaaacg aaagaagaaa ctgaaatcta tttttcagct tcttcttttt
    12181 tattatttat tgttttctgc taaaatctga taaattcgtg tcacatcaga tggtgttccc
    12241 cgtaattcat aatctgccaa gaaaggaaag ccaaatgttt gtgcatattg tttggctgtt
    12301 agacaatact gattattgaa gtttttattt ccgctaccga ctacaccaag acaaagttta
    12361 tgattgtctt catgttccag atattcacgc atcgtttcgg tcaagatttc tgtatcaccg
    12421 ttatctaaac catttccgcc gtctaaataa gtaggaacga aagtaaaaaa tggttctgtt
    12481 tctttcgtaa aatcactatt ttcatggatt tcttttaaag taacttcagg attgacagga
    12541 tcttctgcat gcattttttc agcatattct gctagatgtt tggcaaaggc acgcgtattt
    12601 cctgaaatcg atatatataa aatgttcatg gatcatcctc caattctttc cttttagtat
    12661 agcatctttt ttaagttttt tggtggtgta atttggaaag ggactaggcg ctttgcttgt
    12721 ataatgagaa aaaagccggt agaattatat ttctattaaa gtttttatta attaagtaaa
    12781 aataaaaaag gtttgttata ctttttgata aaagcggttt tattttggag gatgcttaca
    12841 tggatacaga gcaacagaaa aaaggaattg gtttaattcc acttgctgcc ttagtaattg
    12901 gttcagcgat tggaggtggc gtatttggta ttatgacgga catctccgga tcggctggcg
    12961 gcccagctat tttcagttgg gtactcgtag gaacagcaat gttgatgctc tccttgtcga
    13021 tcaataatat caataaaaaa cgtccagatt taaaaggcgg gatctttagt tacgcagaag
    13081 aaggttttgg ccgtttcgct ggttttatca gcggttgggg ttactggctc acatgctggt
    13141 taggcaacgt agcttttgca acattgttga tgagcgcatt aggatatttc ttcccagtat
    13201 ttgaaggtgg gcaaaatctg gcatcgatcg tgatgagtac agtcttttta tggggatatg
    13261 catggctagt gaatcgggga gtcgaaaatg ctagtttcgt caatgctatc gtcacattct
    13321 gtaaattagt gccattattt ttatttattg tagtagctat cagcacgttt tcgatccaac
    13381 aattcattga taacttttct agcgacatta tattgaatgc gaatggacag ccgatcccgt
    13441 tttccagtca agtattgagt tgcatcatgg tgatgctttg ggtatttgtt ggtattgaag
    13501 gagcctctgt cgtaggttca cgagcaaaaa aacaatcaga tgttgggaaa gcaacgattc
    13561 tagggatatt cggcttgatt attatttacg tcttggtttc catgcttcca tatgggacaa
    13621 tgacgatgaa tcagctgcaa acgatcaagg cgcagcctgc tatgggctat gtgtttgaaa
    13681 tgatggttgg taaatgggga gcagtcgtga tcaacggcgg tttgatcatt tctttagttg
    13741 gtgtttggct ctcatggacg atcttaccga ttgaaacaat gggaaacatg gctgaagacg
    13801 gacttttgcc taaagcatgg ggaaaactca acaaaaatgg ggcaccaact tatgctatcg
    13861 tattgacgac gttatgtacg aatgtctttt tactttcatt attattcact gacagtgctt
    13921 ataactttgc ttacacactt ggtacagcag ctattttctt cacgtggctt ttcttaggat
    13981 tgtaccaaat gaaactttct tatcagcgga aagaatgggt ccaattttta gtagggcttt
    14041 tagcaagtgc ctttcaagta tgggctatgc tagtcgtagc ctttaatgaa gtaatgcttg
    14101 ttcttgttct tttcttgcca ggcatttatt tttacctcaa agcacgtaag gagcaaaacg
    14161 agcctgtaca acatatccac ttagataaga ttttacttat aggtgtgacg atggtcggag
    14221 tgattgcaat ggttttattt gccactggga tgcttcacgt atagaagtat aaaagaagta
    14281 taaagagagg ttgtgagcct gtcgtttatt caaaaacata ctaggaaagg agctgtgaca
    14341 caaatgttgt cacagctcct ttttcagaat aaacggtgtt taagagacag ccaatcggca
    14401 ataaggctga acgtctcttt cacagcctct tttttgatga gtcatcatac attttttcgc
    14461 ttaaatacga gatatcctaa tgctaagaac aaaacgatgt aagctaaatt accgaatagc
    14521 atttgattag tagaaagatg agtcaattgc ttcatgatct cttcgtttcc aatttggttt
    14581 tgcagattca acatactgat cggattccat ttgatccact cccatttttg gatgagtgca
    14641 ataaggatac cagatatgat tgacgaagca aaataaaaga cgattcctgc tgagatggat
    14701 gcgccggaac tattgataaa gcaagcaatc atcaacacta aacttaaaat cagccataaa
    14761 ccgatataac tacccaaagt ataagtgaac aagctttgga tcagtgtttg accagtagaa
    14821 accttctccg tgaaggaaat cgaagggaaa agaatcagtt tcatcaacac agtgacaata
    14881 atagctaaaa ggtataaata aacagaataa ataactaatg tgatccattt gcttactaat
    14941 atttctcctc gagaatactt tcgataaagg agatttttca atgtaccata ttgtgcttcc
    15001 atcgaaatga tcgtgcttgc agctgcaatc atgataaaga caatccaaga tgtggcacta
    15061 aaaagctgag cagtcatttc aactggatca atgatcgtcg tgtatttttt ggctagaagt
    15121 gctgttccta tgagcaaaac aactaataga acagataaaa cagcagttga ctttttcttg
    15181 atcaatttaa ataattcttg ttttacaagc aatgtcattt ttcttcttcc ccctttattg
    15241 aatccagaat ttccaataga gaagattcca aatcattcgt cagatgctgg acgtctgtca
    15301 catggatatt ttgtgcagac aatagctgga tcacggttcc taataattct tcgttttctt
    15361 ttaaaagctt gattttatca ggatcttcca aagtatatcc agcttctgtc aagatcgttc
    15421 gagcagctag atcatttgtt gtagagataa cgatgaaatc ttgcgctgtt tggttcaatt
    15481 cagccattgt acattgtttg atgatctttc cttgattgat caggatcagc tcatcgacca
    15541 ttttttcaag ctcgctgagt aagtgactgg agatcaagaa agaggtgccc tgagtagcca
    15601 agtcggtaat gatttttcgc acatctcttg ttgcttgtgg atccaaaccg ttcatcggct
    15661 catctaaaat aacgatggac ggatggttca gtaaggcaag agcaattccc aatttttgtt
    15721 tcattcccaa cgagtaggat ttcgcttttt tgtgaatgta cttttccatc ttgagctgat
    15781 tcacgatagt gtcgatagca gatacatcgt tggtttcaga gaataacttc agatgatcat
    15841 agcctgttaa gaatggatag atacccgggt attcgatcaa tgctcctact tgttccaacc
    15901 ctttataact agagggagaa atctcatttt caccaataca gattgttcca tctgagtaat
    15961 ggatcagtcc taaaatagct ttcatgatcg ttgtttttcc agcgccatta ggacctacta
    16021 atccaacgat tttgccaggg gataagctaa aactgacatc gctgagggct tgtttgtgac
    16081 caaagttctt ggataattct tggaccgaaa gacttttcaa aaatattcac tccttttcta
    16141 tagatagtac aagcatacac ctgatgagtg gagaagtcgc taggtcattg gacttatttc
    16201 ttattttttc ataaaaaaat tgaatattaa ataaaaaggc ttattttttc tgatcagtac
    16261 ttattccaag tgtctagtgg ccgctagtaa tcttgatgcc aatgatactg atgagtagca
    16321 tccccacaaa gaaccaagtg gaaagattca gcttatcatg aaacaagacc acaccaatta
    16381 tgaccgaacc aactgcgcca atccctgtcc agataggata agagagactc atcggcaaac
    16441 ttttgattgc ctttgacaaa aagtaaaaac tagcaatcat gccaatcact gtgtaaaaag
    16501 aataattgat tttagaaaaa ccatcactcc atttcatcat cgttgaccag aatacttcca
    16561 aaattcctgc tatgattaat tccatccatg ccatacttac attcctcctt aactataaaa
    16621 aaatacacaa aaagaggaaa atcatccctt caaagaccta atggaatgat catcctcttt
    16681 atcctacccg gtggcagatt gtatatcttt ttctgtagta tagcacacct atccagatat
    16741 cggcaatctc atacttttta gtaaagtgta ccaagcactg acggatatat cgttagcgga
    16801 aaagaagatg aatgctttgc aatttcatag aaagaagttg ttatttatta atcatttaaa
    16861 aaaaacagta actagacctt atttctatat attatcgggc aataaattaa aaaaagcttt
    16921 accttacaat aagtaagata atttttgtga taaaaaaata acgaaaaata tttaaaaata
    16981 gtgtctattt cgtgagaaaa caactatatt ttttatgtgt tttgctttat aattaaaaaa
    17041 atattgtcat tttatggagg aaaatatatg gaaaattatc ttagcctttt aggcgtcttg
    17101 attgttattc tcgggtttgc atttaaatta gactcgatat tgatcgtaat ggttgcttta
    17161 gttgttacag ctttgactgg cggtcttggc attcaaggaa tgttagaact tttaggtaca
    17221 agttttgtaa acaatcgcgg gatggcgatt tttatcatca ttatgttggc aacaggaacg
    17281 cttgaaagaa atggtctgaa agaatcagcg gcttccttga tcaagcgatt caagaaagta
    17341 tctgctggta tgattgtaga tatttatggg atctttcgaa tgatctttgc ggcttttaac
    17401 gtgagttttg gtggagttgc tggatttgtt cgtccaatca ttttaccgat ggcattgggg
    17461 accatcgaat caaaagggtt agaagtccat cctaagcatg aagaagaact aaaaggtatg
    17521 gcttcagcta tggaaaatgt ctgctggttc tttggccaag tactattcat cggaggagca
    17581 ggggcgttgc tagtccaatc aacactgaaa gatctaggat atgacgtaac attgggacag
    17641 cttgctttag tggaagttcc agtggctatc acggctttag ttgtggcaag tgtctacttc
    17701 tatcttaaag aaaagaaatt agcgaagaaa tattatccag gaaaggggca ataagatgga
    17761 cttttttaca aatcctgata ttttattagg cgataaatta ctggagatcc tttatatcgt
    17821 gatgggactc gtattgattt acactggagt aagaaattta acagataaaa ccaatcctca
    17881 tcgttatggg tcagcctttt tctggacggt cttaggggta gttattgcag gaggaagatg
    17941 gcttccggca atcgtgaacg gtggcttgat ctttgcgatg accataccag caattgctaa
    18001 gaaagtcagc aaaggagaaa gccggcttcc atcaaaagaa tatatggaaa aaatgtcaga
    18061 caaactagga atgaaaattt ttattccagc tttgagtatc ggtgtctttg cgatcttgtt
    18121 tgctctattt accaatttag gtgctttagt cggcgtgggc gtcggtgttt ttgcagccat
    18181 gctaatcttg atgttctttt ctcgagataa tcgaccaagt acatttttag atgatgcagc
    18241 agatatgctg ggcacagtag ggccgttaag catgttgcca atgcttttag cttctctagg
    18301 tgcggtattt acaagcgctg gagttgggac agtgatttcc aatgcagtag gtacgatcgt
    18361 tccagaagga aatgtgaaca tcggcatcat catttacgct cttggcatgg tgatatttac
    18421 ggtaatcatg gggaatgcat ttgcggccat caccgtgatg acggttggga tcggtgcacc
    18481 attcgtattt agtcatggag cgaatccagc gttaatagga atggttgcat tgacttgcgg
    18541 tttttgtggg accctgctga caccgatggc tgctaacttc aatatcgtgc ctgtagcgat
    18601 gctggagatg aaagacaaat acggagtgat caaaaaccag ttgtttattg cgttatttat
    18661 gctagttttc caaattgctt atatgattct atttaaataa tagaagagag gggttgtgac
    18721 agaataattg tcgcaacctc ttctaacatt gtagtggaac actatttttt tataagaata
    18781 tattaagttt gatctgttat gttatttaaa ctattgtttc ctttatttac gctgttcaag
    18841 gaaaatggta tgataaaaga taaccatatg ttaaatcaat cggacggagg aacaacagat
    18901 gaaagtacta gtaacaggtt ttgatccatt tggtggggac aaagtgaatc cagcctatga
    18961 agcagtaaaa aaaatgccag atgaaatcag cggtgctgaa atcatcaaag tggaagtacc
    19021 gaccgtattc gaaaaaagca gtcaagtagt caaagaagcc atccaacagc accaaccaga
    19081 tgtcgtgatc tgtgtcggac aagcaggtgg gcggtcagca gtctcgttcg aacgtgtcgc
    19141 aatcaatcta gcagaagcaa gaatccctga taatgaagga aatcagccat tcgatacggc
    19201 tttagaagaa aacggtccag ccgcttactt tacctcattg ccaatcaaag caatgacaaa
    19261 aaatgtacaa gatcatggtc taccagcgta catctcttat acagcaggaa catttgtctg
    19321 caatgatatc atgtatcgtt tgcttcattt gatcgaaaca caattcccgg caattcgagg
    19381 tgggttcatc cacgtgccat tctcacccga tcaagtgatc gatcgtccag tgggtacgcc
    19441 ttctatgtct ttagaagaca ttgcttcttc tttgacttat gcagttgaag cagcagtaga
    19501 aaataaagaa gatatttccg gaaatgccgg aacaactcac taatttagca agaaacactg
    19561 gctggttgct aacgttatgt tagtagccag ctttttgtaa ttagaaagga aagaaacagc
    19621 cgtggaagaa tggcaatccg tctttgaaga atggtttcca aaagaaatca gcaaaagcta
    19681 tcccatcaaa atttctaaac aatacacaag cagtcaacgt tgggagatct atgcaaaatt
    19741 aacgaaaaaa cagcgggaac tagtcgataa gcaccgccga tatctaatca gctctcgttt
    19801 tatggaagaa cattatttag ctgcaacaga ttgggtgttt tctgacttca agatcaatcc
    19861 gttttttcga acaaaacgaa gccagcaaaa attgtattgt gagtgtggta gagaattaaa
    19921 agtgcaatac atcgttaaat cgccaaaaac cggaaagatc ctaaaactag gcatcaacca
    19981 ctttgccgat cacctgcatg tctctccgac agtggcagct tcgatccatc agggaatgac
    20041 aaaagttgat ctggccttag acgaattatt atggcttaag cagaagaata tcgattttcc
    20101 tgaaggacta tggcaaaaat actgttttgt cttgtaccaa aatcgtcgga tgaaacagcc
    20161 atatttaccg gatataaaat tagctcagcg gctcgcagaa tttcgccaag tagaaatgcc
    20221 gatttacata gcggattatc aagcattgga aaatgaaatc aagaaaataa gtgaacacat
    20281 caatgggcag tcaaaaaagc gacagataaa aaaagaacta tttgacgact ttgcagaaga
    20341 attagtaaag gatgtagaag aattcttgat caactatcgc gcatttttac gtaaagattg
    20401 gcaatccatc gtatatgaag aagtgccagt acatcctaat gcttattttg aaacatttat
    20461 ttccgtgttg agaaagacaa aacgtcaacg aacaccagaa gtcactgctc agatggaata
    20521 ttttgcgaaa aaccaacgat tcatccaacc aaaaatctat ctatttattt ggaagcaata
    20581 ttgtcgctat ggttttacag aagggttctt cgacagcatt cctcggatag tcagaaatgg
    20641 ttttttaaaa gtattgcgta aagaacgaga agctatacaa tctgctgata agaaagaccg
    20701 tacagtctca aaagaaaaat ggcaactagt agtgaaagat atccaatctg gaaatgtgca
    20761 agagacaatc gacaaatgga aaggaaaaca ttatcgtttt actgaagctc agaaacaagc
    20821 gttggaatat tatcagaagt tagaagaatc actgcgtttc aatgatgagg caagaaagta
    20881 tctgaaggaa ttactataaa aaa
//
LOCUS       DACEUZ010000037        20785 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.037, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000037 DACEUZ010000000
VERSION     DACEUZ010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20785
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.037"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(6..1208)
                     /locus_tag="IXG44_001184"
     CDS             complement(6..1208)
                     /locus_tag="IXG44_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1657916.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            1325..1840
                     /locus_tag="IXG44_001185"
     CDS             1325..1840
                     /locus_tag="IXG44_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ1657917.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(2039..2485)
                     /locus_tag="IXG44_001186"
     CDS             complement(2039..2485)
                     /locus_tag="IXG44_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAQ1657918.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(2532..4010)
                     /gene="msr"
                     /locus_tag="IXG44_001187"
     CDS             complement(2532..4010)
                     /gene="msr"
                     /locus_tag="IXG44_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAQ1657919.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(4364..4993)
                     /locus_tag="IXG44_001188"
     CDS             complement(4364..4993)
                     /locus_tag="IXG44_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAQ1657920.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            complement(4977..5441)
                     /locus_tag="IXG44_001189"
     CDS             complement(4977..5441)
                     /locus_tag="IXG44_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ1657921.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(5928..7196)
                     /locus_tag="IXG44_001190"
     CDS             complement(5928..7196)
                     /locus_tag="IXG44_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosaminidase"
                     /protein_id="HAQ1657922.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(7427..8164)
                     /locus_tag="IXG44_001191"
     CDS             complement(7427..8164)
                     /locus_tag="IXG44_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ1657923.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(8231..8800)
                     /locus_tag="IXG44_001192"
     CDS             complement(8231..8800)
                     /locus_tag="IXG44_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HAQ1657924.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            8928..9800
                     /locus_tag="IXG44_001193"
     CDS             8928..9800
                     /locus_tag="IXG44_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ1657925.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            10142..11014
                     /locus_tag="IXG44_001194"
     CDS             10142..11014
                     /locus_tag="IXG44_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1657926.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            11011..12969
                     /locus_tag="IXG44_001195"
     CDS             11011..12969
                     /locus_tag="IXG44_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1657927.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(13220..14071)
                     /locus_tag="IXG44_001196"
     CDS             complement(13220..14071)
                     /locus_tag="IXG44_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ1657928.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISILAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            14194..14661
                     /locus_tag="IXG44_001197"
     CDS             14194..14661
                     /locus_tag="IXG44_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HAQ1657929.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(14924..15406)
                     /locus_tag="IXG44_001198"
     CDS             complement(14924..15406)
                     /locus_tag="IXG44_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ1657930.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(15506..16369)
                     /locus_tag="IXG44_001199"
     CDS             complement(15506..16369)
                     /locus_tag="IXG44_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ1657931.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            16967..17815
                     /locus_tag="IXG44_001200"
     CDS             16967..17815
                     /locus_tag="IXG44_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1657932.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPHFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKLKKR"
     gene            complement(17986..18327)
                     /locus_tag="IXG44_001201"
     CDS             complement(17986..18327)
                     /locus_tag="IXG44_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
                     /protein_id="HAQ1657933.1"
                     /translation="MVGLKRKELAGRRFTRIVSVGGGAKNLAWLQIQADIFDATVYCL
                     SVEEGPALGATMIAALGLGWFDTPESCCQNFIQYTDAAEPLLQNVEKYHKIYQQCRKI
                     YPATKEICHDL"
     gene            18413..19120
                     /locus_tag="IXG44_001202"
     CDS             18413..19120
                     /locus_tag="IXG44_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HAQ1657934.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLVYAGSAQFITVSMLASHSPLLSIVFSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVIVV
                     VTLIGCGLGVWIKHAFF"
     gene            19107..19430
                     /locus_tag="IXG44_001203"
     CDS             19107..19430
                     /locus_tag="IXG44_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAQ1657935.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRVLPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLAGILSLS
                     LIRLL"
     gene            complement(19519..20556)
                     /locus_tag="IXG44_001204"
     CDS             complement(19519..20556)
                     /locus_tag="IXG44_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ1657936.1"
                     /translation="MSEKELMKVVGFYEGLPIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGSGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHML
                     GKVVVSGPFNG"
BASE COUNT         6194 a         4299 c         3689 g         6603 t
ORIGIN      
        1 atttcttatt cttcttcatt tgtagcaaaa taattacggt gataaatgat acaacataca
       61 ctgataagaa caatggcacc tgcaaaataa aacgcatagc tttcaccaat atgttcacca
      121 atcacacctg caataaatgg tgctaaagct ccaccgaaaa agcggacaaa actatagctg
      181 gacgaagcaa cgtttcgttg cagacctggt atttccatag caatcgtcgt caacagtgta
      241 ttgataattc cttggaaaaa tcctgctaac acaatgctga tcggtacaag gactggcata
      301 gaagttccag ctcctaataa cagcaaacaa ataatgaata agacaaatgc tgctaaaatt
      361 gtccgataag tcgtaaactt ccgctcaaga gccggtgcaa cgaaaatcga agcaatagcc
      421 agaagcacac cccagccaaa aaatacaaaa ccgacttcta attcagaata gccaatcaat
      481 aaaaatggcg catacgcaag taaagtaaag aatccgaaat tatataacaa tgcgattagt
      541 ccaactgaac gaagtttatg attgcttaac gctttgacac cttcccaaag gcctactttt
      601 ctctttggtt tctcagtttc aggtaaaagt accgctaggg ctataaaacc gcaaaacatc
      661 aaaaatgcta ccccaaaaaa tggataacgc catgaaccac tgcccaagac cccaccgact
      721 agagggccaa cagccattcc gcagccaagt gctgcttcat acatcatgat tgctttttct
      781 gtgttaccaa ctaaaatact gacgatcgtt gctaaagcag tagagacaaa taaagcattc
      841 ccgacacccc agccggcacg taacccaatc agcattccga tattcccaga taatccgcca
      901 agaccagcga atacaacgat gataaacaat cctgtcatca atgttttttt caaaccgatc
      961 cgactagata agaaacctgt aaatagcatc accacaccag tgactaacat gtaactagta
     1021 aataacaggg tcgtttcagc tggcgtagcg tttaactgtg cagcaatcgt ttttaagatt
     1081 gggtctacta gacccactcc cataaaagcg atcatacagg tagctgctac aactagcgct
     1141 gaacttttcc cctttgtttc ttcatttgtt tgttcttgta tatctatttg attctccaat
     1201 atttccatcc ctttctctct attagcatca tacatgattt attttacatg taaatatgca
     1261 catgtaaaac gtggaaactt tactgtgata taataaagaa aataagacga gaggtaggat
     1321 ttttatgaat actttagcct atatattatt atgcgtgctt ttatccaaac cacgttctgg
     1381 gtatgaattg aagcagctga tcacaatttt ttgggaagcc catcacagtc agatctatac
     1441 aacgcttgcc aagttagacc agcagggtta tgtgaccgtt ttggacaaag acgagcactc
     1501 tcaaaaaaag atctaccact tgactgatga aggacgtgct cttgtagagg aatggatcaa
     1561 agaggaaact cccgctccta cgcaaaaaga tgaatttcta gcaaaaatct atgctttttc
     1621 gacgctcgat aaaacaacag ctactggact gctattcact agagaacaac agctgaacaa
     1681 aatcatgcaa aaaaatcaaa aaaaattgaa tgacctctcg ctagacaaga aacaagaatt
     1741 tggacgttat gttgtgatcc agcgcagaat tatgctttgt cagcaggaac tccaatggtg
     1801 tcagcaagta agagaacaaa tgcagcagtt ttttgagtaa gggataaaca gaacctttct
     1861 agaaatatct attgaagagc aaattatctt caataaaaga gcaatacagt tggaaaagaa
     1921 aaagcgatcc gaacgacagg ccaaaaccta tcgttcggat cactattttt ctagaactgc
     1981 attcaagaga ctatgacaaa aagcatgcca aggccttttt tcaaataaca gtgttccatc
     2041 attcattact ttgaattgct tttagcacac ttcttgaaag cgggccaacg atgatcaact
     2101 gcaacggtaa agcggcaatc acattcattt tccaagcatt catataagca gagaataatt
     2161 gatcaggtat tccttgttcc atgatcacac cgaataaaga catacatgtc accattccta
     2221 aaaccatcaa gcatgaaata gttaagatca tttggacctt gctttctgga tttactggta
     2281 gtttaaaggc aatttttttc gccagcacac cgatgatgaa gacatccaaa atgaaagcca
     2341 ccacgaatcc aggcactaga ccaatcaaca aacttgataa atgaagatct tgatgcagcc
     2401 ataaattata cgcgctcatc cccaagacca tcaaaaaaca catcattgtt gtaaaaatta
     2461 ttccttcttt tttatttgta ggcaatctgt accactcctt taaatttgtc acgaaaatca
     2521 tcgtagcaca tttaaaaatt tctcgtaagt acttttttta ttttctgccc ttttatttca
     2581 taaacttcat cagccgtacg ctcgacaaag taagaatcat gagaagtaaa caaaatcgtt
     2641 cccggatata tttgcattag cttctctaaa gcttcgatcg ttgccatatc aataaaatta
     2701 gtcggttcat ctaacagcaa cacattactt ggcttagtaa aaagcaaagc aatcgtcaga
     2761 cgggtcgctt ctcccccact taatgtacat aacggtttcg tcaaagtttc ctgagcaaac
     2821 cctaagtgat tcagcaaact acggacaagc gattctgaaa actctgtttg tctcatgaca
     2881 aaacgaatga tggattcttc agatgtcatt tgataaccct tttgctgata tacttgaaag
     2941 cttactttcg gagagagaag gattccttct ccttgtttgc ggatatgttc taataaggtt
     3001 gtcttacctg aaccattttc accgacaatc gcgatttttt tacctaaaga aaactgaaaa
     3061 tcaccattta ccagtaatgt tctcccactt ctttccaatt ggacattttg tcccatgatg
     3121 ggatagcggc tgtggagttc aagagatttt ggtactggaa aacggatttg tttcatctgc
     3181 tgcggcttgg ttgtttctcc tattttttgg agtcttctct ctaatgtttt cgcattcttt
     3241 tggatggctt tttgtacact gtctttttgt ttagaggaag acaaccgatc cggtctgatt
     3301 tgttgctttt tctttttact ggacactttt tccaactttt ccgcttgttt tttcttttct
     3361 tggatcgatt tttccaatcg ttttttctct ttcatgatcg actctgcttc tcgtagctga
     3421 gtctttttct ccaattcctt ttgagtgaga taggcactat aattccccga aaactctcgg
     3481 atttctccgt cttttacttc ccagattttc gatgctagct catctagaaa atagcgatca
     3541 tgactaacaa aaaccagcgt accataataa taacgaagct cttcaatcag tcgatcaata
     3601 ctttttctat ctagatgggt tgttggctcg tctagaagca tcccttgttc ataagaagaa
     3661 agaagacgta tcaactcgat ctttcgttct tcccctccgc tccatttccg ctctgaagtt
     3721 tcttccagtc ttaactggaa atgactggct aattccaaat ttaggtcctt tgcttcagcg
     3781 agataggtta attgaggcag gtagttgaag gtgatctctg tttggatttt cccatgatcg
     3841 ggaaaaagtt caccggctat tagtttgagc aaagtcgatt taccgacacc attttctcct
     3901 atgatcccta tacgatcttg ctgatagaca cgaagagaat caatagatag ttctagctgg
     3961 ttgccaaagc tgacttgtag atttgttata tttactgcta aattttccat atgttttaca
     4021 ctcctatttc atgtaaaaca acaattatcg atactaaaaa agagcagaaa aatctgtttg
     4081 caaaaaaact gcatcacatc ttttcctact ctgaacgaaa taactatgac taaatcacca
     4141 aatctgtcat agctttcacc tattctactg tcgttcgaaa aaatggacat actccatcca
     4201 tgaaattgtt gtttatacat gtcttttgaa tttatttagt tgttattcaa aagttcgaaa
     4261 cgtaatttca tcgatgcagt catttttccg acctcctttt ttattaggtt accacacacg
     4321 attcaaaaaa agcaatagct actgaatagt aactgaaaac cttttattcg aacgtagtga
     4381 tcttttctgg tgctaaacga acagactcgt aaccttcttc taccgctttt cctattgata
     4441 aaataataac tggaacataa cggtctttat ccaaaccaaa tgcctctgcc aactgatctg
     4501 cttcgaatcc accaattgga tttgtttcat agccatgatc acgcgcaact aacatgaatt
     4561 gcatagccgc taagctagaa tcgactttga ctacatcgtt catttcttct cttgaaaaac
     4621 tcttgtaata aggaatgatc gcagctaatt gttgatctct gacttctttt ggcatcttcc
     4681 ctgattcgta agcttggtta tagatttctt cgccatattc gtaacattgc atatcgccaa
     4741 aaatcatgac cattgcagaa gaagtatcat tttgtctcgt attgaaacgg atcaaaggac
     4801 gcaagatttc ttttgcttcc tcgctttcag cgaccacaaa gcgccatggt tgcatattga
     4861 cagaagaagg tgcagtcgtc gcttctttga tcatttccag catttcttcg tgtgggattt
     4921 tgtatgtttc atcatatacg cgaacggatc ttctaccagt cataattgtt gaaaaatcat
     4981 ttttttgttc cattattatt aacctctttc gtttctgttg ttatattgca aagttgggta
     5041 ttcaatttat ctaaaagtgt tagaaattgt ttcatttcta catcgctgaa agccggggat
     5101 agcacggaac ctgtatcttt atggtccatt tcacaatgct cgattttctt ctgggctttt
     5161 tctgttagcg ctacatacac ttcccggttg ttctgaggat ttctctgacg cgaaacatag
     5221 ccatttgctt ccaatacctt cagatgacga gtaactgctc cttgatcaat ctgcaacttt
     5281 tcttgcaaac tgctttgtag caatggattt tcttctttca gtctcatcat gatctcgtat
     5341 ctagtaagac ttataccgat attttcttca aagatctgga cgattttttg atcggctaat
     5401 ttgatttgat ataaaaggtg actaatctct tctaaagaca taatttcctc ctttctaata
     5461 attgactcgt caacgattga cttgtcaata atagaataaa aaaagaaaaa agtcaaagaa
     5521 tcagctgtgt aataaaaaaa acgctctcaa tagaaaagta tagagaaaag aaattttttg
     5581 tagaatgaac ggaaaaaatg ataaagaaag cacaacgcta aaaccaagat tattgattct
     5641 tatcaaacta tagaagatag ctcttctgca gtagaaaatt ttacaccgcc aaaaatttcc
     5701 gaaatcgcct tttgcaagaa tcccgtcgaa tattagaaat tggatcagta tgcttaaaca
     5761 gtaataaaaa agttatgaca agatgttgtc acaacctttt ttgagtaaac ggtgttcaaa
     5821 aacttgcatc tgcggtaata agttcaacca aacaaaaaac atgaaaatct gtttttgttt
     5881 ggctgttctt actacttgat gcagggcagc tttttcacta cctcttttta ataaccataa
     5941 aatcctattc cttcatatct ccagcccatc ggtgttagcc aatctctttc cttagcactc
     6001 aaggtatagt gatgggtacc agttttttca tttggattaa atagtcgata aattggcttg
     6061 gaaccaccag aataaaaact tagaccttca taacgccaac cgatttttgt taaagagttg
     6121 atctcagatt gtgccattgt gtaatgatgg tctccactgt aaggattgta taaacggtag
     6181 acaggttgtc cgctgtttgg tgcttgccaa gcaatccctt catattccca gcctacagtt
     6241 ggcagataat ttttttctcc ggcattcaat gtatataaat gttctcctgt atgacggtta
     6301 tacagacgat acatcggaga agttgaagtt ggtgctgtat catactgtgt cagattatag
     6361 gtttcgatta cattgttcaa tgtagaacca taattaggcg cagtggcata acgacctgtt
     6421 agccaagccg tagcatcttt gtaagagttc gtattgcttt tccatgcacc ggaatagtag
     6481 taaacgcctg gctgaaaaga agtcgtcttc aataccgtta cgttatcttg caatgattct
     6541 ttgtacgaag gatattttct aaaagcggca ttcaccgtaa tccattgccc attcacatat
     6601 tccgaagtag gcatcgtaac agattggcca ttatagcttc ctttaatccc aaatagattg
     6661 taattagggg cagaagccaa agtactgttc ccccagccac tttcaacgat ggcttgagca
     6721 atcatgactg atgcatataa gtcattggca cttgccaaac tttgtgcagt tgaggcaata
     6781 ctatcaatga acgcttgttg agaattagca gatgagcgag cataaaaagc atagccagta
     6841 tagttttcga attcagacaa atctgcttga tgggtatgtg tcaatgtttg cccatctgtc
     6901 aattggttcg tgatttcttc tgcattctct ggattgttct gagcttctgc tgatgtactg
     6961 tcttcggttt gtgtttgttc tgttgtttca gttgttggtt cttcggttga aattgtattt
     7021 tctgagacag tagctgactg caaagtactc tctgtatcgt tttcaattcc tgtttctgaa
     7081 gaactcgatg tctgagttgc gacagtttct gtcgattctt cagctagcac tgttgtgttg
     7141 gcaactgcta gatttcctaa aagaataacg ctaaataata aattctttgc tttcatcttt
     7201 tttctccttt gaattatgaa acttttccta aataacagtg taactaaaaa agtaaaagca
     7261 agtcaatgtc catatttaaa taacatttgt ttctttttga taaatttgct ttttctacat
     7321 aaaaagacgt aaatgaagaa tattcttctc cattcacgcc ttaaattgat ggtcgtttga
     7381 ataactattt aacaagtaat tctctttgga tattatccgt tatggattat acttcctgtg
     7441 tcgtcggacg gataatcatg ttactgacag acatccggtc tggcgtttct atcgcaaatg
     7501 ctacagcaga agctatatct tcggaggtca atccccattc tttttgcgct tcatgaagtt
     7561 caagagcagc ctttttgtcg ctgatggttt gatataattc agtttgaaca gctcctggag
     7621 agatgatcgt agatttgatg ttattttgcc gttgctcttg acgtaatcct tccatgattg
     7681 cacggacagc aaattttgtt ccgcaataaa cggcggaatc aggataaaca acatgtcccg
     7741 ccacagaatc agttgaaatg atatgcccag atttttgttt ttccatgatt ggaagtacgg
     7801 cggaaattcc atttaacaca cccatgatgt tgatatcaag catattttgc cattcatcac
     7861 ggtgaccttc aactaatgga gcggttggca tgattcctgc attattgtac aatacatcga
     7921 ttcgaccgaa cttttccatc gctaaatcga taactgcttg gacttgtgca aaatctctta
     7981 catctgctgt ttgaatatat agctcagctt ctggtaaaga ttcttttatt gcttttaggc
     8041 gatcttctcg gcgtgcagct ataactagtt tagccccttt ttctgctaat agcttcgttg
     8101 ttgcttcacc gattccgctt gaagcgccca tgatgacaat cactttttct gataaatcat
     8161 tcattctatt ctcctcctgt tttgaaaaat ctcttgctta tactcactat ctcttatttc
     8221 atcttactgt tcaaataatc cgttcgacag gtttgacgat tttggcaggg attcctgcaa
     8281 cgatggtatt gtctgggaca tctttcgtaa cagttgcatt cgctgcaaca atggcattct
     8341 cgccaatcgt tacgcctggc agaatagttg ctcctgctcc gatccaagca tttttcttga
     8401 tgtggattgg ctttactgac aagcctcttc gttcttttgg atcggttaga tggttcaccg
     8461 tgatgaggtt gacacgagga ccaatcaata ctgaatcttc taaacagatg ccacctaagt
     8521 caacgaacat cacttgacga ttgataaaga catttttccc tatactaata tgtttgccga
     8581 aatctgtata aaaaggcagc agaacagtca ccgtatcatc tactttgtca ctaatgatat
     8641 tttctagccg tttgcggata tctgctggtt catggtaccc agtattcagc gctgctaaag
     8701 gctcttcgtt ttcagcttgg atctgatgga tcacttcaaa gagcggatcc gttactcgaa
     8761 tctctttatt gacgatttgt ttttcgatga ggcttgtcat tttgctccct ccttttatcc
     8821 ttactttata acggacggct tatcatgtct aatacttata aaacatactt aactattcct
     8881 tttaactata cttagtggta tgctttttta aaaacaggag ggaaaaaatg gatattcgta
     8941 tactgacgta ttttttagca gtagctagag aaaaaaatat cagccgagca gcaaaatctc
     9001 tgcatatcac gcaacctact ctctcaaaac aactgaaaga tttagaagaa gaacttggtg
     9061 tgacactttt cactcggggc tcccgagaga tacagttgac ggattctggt cgatatcttt
     9121 attcaaaagg aaaagaaatc ttgtcgttgg tagaaaaaac ggcgaacaat ttaacaaatg
     9181 acgcaatcat ctctggagaa atctacattg gcggaggcga aactaaagca atctctttca
     9241 tttctgatag ccttcattcg ttgatcaagg cgcatcctga tatccagttt catttatata
     9301 gcgggaatgc agatgatatc atagacaaac tggatagcgg attacttgat tttggactcg
     9361 taatcgaacc gacaaacaaa caaaaatacc agtcatttcg actgccagcc caagatcgtt
     9421 ggggactttt gatacatcga aatcatcctt tagcaaagca aaccgtcatc catccgcagg
     9481 aactgaaaac tgttcctttg atcatttccc ggcaaacgtc agtggacagt caactagctg
     9541 aatggctagg tttcagcata gatcagttat cgatcgtcgg aacatataac ttattgtaca
     9601 atgcttcttt gatggtcgat aacgggactt ttggtgcatt agctctagat gggatcatca
     9661 atacttatgg gacggaaaaa atattcatcc cactcgatcc gccattacaa gcttcactga
     9721 atctcatttg gaaaaaagac cagcctcttt ctcctgctgc tcgtaaattt ttagatgtcc
     9781 taacggagaa atacagttaa gtagagtttt tttccttcgc tttcagcttt ttaacaacga
     9841 ataagataag caaaagatcc aacgattaaa taacataagt ttcttaattt atttcttcct
     9901 tttatttctg ataattccag taaattacta taattataaa aaaagaattg acaaaacata
     9961 actgtctata taaaattgat tacgttaaca ataattcgtt tcatgtgact agatcatact
    10021 aggcatgaaa aatctctgag gaacatggtt tccctaggag gtttttcgtg tctatttttg
    10081 tttggccgca atcaactggt ctagcaaacg tgaaaaccaa atcttcaaaa agggtgcgcg
    10141 catgaaaatc aaaaagatct ttaatcaaaa tgcagtattg gtagatgacg gtggacagga
    10201 aaaagtagct attggaaaag ggattggatt tgacagaaaa agaaatgatt tgatttttga
    10261 tcgtgatgtc gaacggatat ttgtcatgga accagaaggt caaatcaagt tgcaaaattt
    10321 acttaatcag attgatgaac gctttttatt tgcagcagaa acgatcattg atcatgcgga
    10381 aactgttttg atggaaaagt tgaatgaaca tgtattgatc gctttgaccg accacattgc
    10441 tttttctgcg gagaacatta ataacggtat cgtcatccgc aacaaacttt taagagaaat
    10501 cgaagtgctt tacagtgatg aattcagtat tgctcaatgg gcagtagagt atctgacaaa
    10561 agagcttggg attccatata cttatgacga agcaggatat atcgcaatcc atattcacag
    10621 cggacgaagc ggtcggaaca ataaccatcg cagtatccgt gaagtgacga ttgtatctga
    10681 catcatccat ttagtggaat cagaattgga aacagatatc cattcagaac cattttcact
    10741 gaattattca agactggtga atcatttacg actgcttcta caacgaaccc atgcgcaaca
    10801 atatgctgtt ttagacacgg aaatcgtaga aatggtcaaa cgaaaatacc cagaaagcta
    10861 taagatttct aaaaaaatcc gtgtgttgct gaccaaagaa taccagctcg cgatcacgaa
    10921 agaagaactg ggttacttgg ccatccatat cgagcgttta agaagcgcaa tcgtacaaac
    10981 aaatgttaac aatcatgagg aggaaaaaaa ttgaaagcat atctacaacg aatgggacgt
    11041 tcccttcaac ttccagttgc ggtcttacct gctgctgctt tactagtagg gatcggaaac
    11101 tggtgggcat cttatagtaa cgacattgtc gctcattttt tacaagctgg cggtaacgca
    11161 gttttaggtc aattaccttt gctgtttgcc gttgggttag cactagggat gtcagtggac
    11221 aaatcaggtg ctgctgcctt ggcaggtttg gtcgcatttg aagttccagt aaatgtttta
    11281 aaaactgatt cagtagctac tttattgaat atcaaaccag aatcagttga tccatctttt
    11341 gctcagatca gtaacgtatt tattggtatt attgccggat tgattgcggc agctctttat
    11401 aatcgtttcc acgaagcaaa attaccgatg gctttatctt tctttagcgg caagcgtcta
    11461 gtgccaatct tggctgcctt tgtcatgttg ctgatcagtg cagtgctact gctcgtatgg
    11521 cctccggttt actctgcttt agtttctttt gctaaattca ttttgagttt aggctggggt
    11581 ggtgcaggtc tttatggctt cttcaaccgt ttactgatcc ctacaggatt gcatcaagca
    11641 atgaactccg tgttctggtt tgacgtagca ggaatcaatg atattggtaa atttttggct
    11701 tctgaaggaa caaaaggcgt aacaggaatg tatcaagctg gatttttccc aatcatgatg
    11761 ttcgggcttc cagcaggagc atatgctatt tacagaaatg cacgtccaga aaacaaagca
    11821 aaaacagctt ccttgatgct agcagcagct tttgcttcat tctttactgg tgtgacagaa
    11881 ccacttgaat tttcatttat gttcgttgct tggccattat atgtcttgca tgcaattttc
    11941 actgggattt ctttagcagt gagtgcattc ttccattgga cagcaggatt tgcctttagt
    12001 gccggatttg tcgattactt cctatctttg aaaaatccga ttgcgaatca gccattgatg
    12061 ttgatcgtcc aaggattagt aacagcagtg atttactact ttggctttaa ctttgctatc
    12121 aaaaaattcc atctgatgac tcctggccgc gaggaagcag atctttcaga tgacgatact
    12181 gcaacgacta attcagacaa taaatatgcg gcacaagcaa gcaaaatcta cgctgcatta
    12241 ggcggagccg acaatgtaac ttcgatcgat aactgtacta cacgccttcg tttgcaagtc
    12301 aaagatacag gcctgatcga tcaaaataaa atcaaagcca ctggcgtacc tggtatgaag
    12361 atcatcgacg gaaaaaatgc gcaagtcatc gtaggtacag aagtacaatt cgttgcagat
    12421 gagatggcaa aactacatgg tggtgctgca gcacgacctg cacaaacaaa caccgtggtt
    12481 aaaacagaaa catttgccgg tgaaacgatc gaacaagata tttacgctat cgcaaatggg
    12541 aaactgattc caatcactga agtttcagat gatgtattcg cggaaaaaat gatgggtgac
    12601 ggctatgctg tattaccaga aaatggagaa atcttctctc caatcgctgg tacgatcaca
    12661 aacattttcc caacgaaaca tgctgtcgga atccaaacag atgctggtat cgaagtcttg
    12721 cttcatatgg gcatcaacac tgttgactta aaaggcgaac cattcacttt atatgtagaa
    12781 gaaggtcaaa aagtcgcaag aggtcaattg attgctttag ttgatcttgc cgctattcaa
    12841 tctgcaggca aaaacacgga tatggtcgtt gtattcacaa atggcgacaa agtccaaagc
    12901 ttagaaatcg aacccgcacg tgatgtaaaa gcaaatgata aaatcggctc tgtttccaac
    12961 aaagcctgag ccatcgtctt ccccagactt ttttatcgat aaacaaacag gagctgagac
    13021 aaaaatcttg tctcagctcc tttttccgaa taaaaacggc gttctcctgc caactccgcg
    13081 gtaataagtc cagctaaacg aaacatatga agagctattt tttatttgac tcttcttatt
    13141 acttgatgca gaacagcttt ttcacaaaat ttttcttcat actcaaaact aatcggcttt
    13201 ttcacaacct ctttatacat tattcctgat ccaatatttc ttttaatgct gcttcaagag
    13261 aagttaatgg ttttcccaat actttttcta aatcatcaga ggaaatagct aactgatcct
    13321 ttttaatatc atgttggatc gctaaaaaca tgtcagctgc ttcttgaggg aatccggctt
    13381 cttttagatt ttcgacaaac gctgcatcac ttgcttcaat aatttctaac tctttacctg
    13441 tcgcacgctc taatgccttg gctaattctt catagtcagt tagattgccg gataattcta
    13501 agatttctgg agagtcactt tctactagta cttttgctgc agcttctgca tattcacgtt
    13561 tcaatgccca acctgttttt ccatttcctg cagcataaac gaattgacca gtttttaaag
    13621 cctgcccaat gataggtaat tcattctcta cataccaatt attccgtaaa aatgtatggg
    13681 aaatacctga atccttaatc attttttcag tgaattggtg atctggcgct aatatactga
    13741 tcgagtgatc ggcatctgca aaacttgtat aagcgatata agatacccca gctgcttgtg
    13801 ctgccttcac tacattctta tgttcggctt gacgatttcc tggaacgcca gagacaaaaa
    13861 gcaatcgatc tattcctttc aacgcttctt tcattgagtc taaatcatca taatcaccaa
    13921 tacgtatatt aaatccagct tctttcagtg cttctccttt ttcttcacta cgtactagta
    13981 catagatttc attttgtgga accatctttt ttaagaattc cagcgcataa ccaccaaaac
    14041 ctccagttgc tcctgttact aaataatgca tatttgccac tccctattct ttttatttgt
    14101 taagatttac agctgtacat ttataaatta cacctttatc ataaagagtg tggtaagata
    14161 agtcaagcaa aaaaaatgaa aggatgacaa ctgatgaagt attctctgca atttagtgat
    14221 gcaatccatg tacttgccta tattgaaatg tttcaagata cccgatcact ttctagtgag
    14281 atgattgcca gtagtatcga aacaaatgca gcaaatgtac gtaaaatcat gagtcagctc
    14341 aaaaaatccg gtttgatctt tacaaaaacc ggaaaaccgc agccttccct tgccaaaagt
    14401 ccagatcaga tttctttact agatgtctat cgcagcatcg aaggtaatac acagcttatc
    14461 caagtcgatc caaaaacaaa tccaaattgc gtcgttggtg cgaatatcca agaagcttta
    14521 gaagaaagct acgctcggct gcagaaaaag gcagaagaag aaatggctaa gatcacactt
    14581 gatagtctga tacataaaat agccgttttg gaaaaagaaa aaagaccaga aaacacaggc
    14641 ctcgtagaga gatttttata attctctcta ctgtgcctgc atttttctga tcggaatcgt
    14701 ctacataaat tcttttattc tccgggggac aaaagccttg tcatgcccct ttttcctctc
    14761 taatgctcag gtcaaagcgc tgaagtcatg acgtcttgat aaacggtgtt caagtagctg
    14821 accctcggca ttagttctga aaaatgcaaa atatggaaag ctatttccca tttttcctct
    14881 ctaatgctca ggtcaaagcg ctactttcac gacctctata ttatcatggt ttattgacta
    14941 ccggtatcca gatttctccc atatatttcc cgttggtaac tcccatttcg actacagcat
    15001 ttggaccccc aacataggcg taatcttcta cttctcttaa aacatgccca aaggccgtat
    15061 tcgtcaaatc atttcttaat tcttcaggcg attctgctaa agcgcttaca acaagatacg
    15121 ttccttttgg gaatgggatg attcgcgtag tgtttggaac atcctgactc gtttgaacac
    15181 cgacatagta catcattttc ccgttatagg gttcgttgac tgtaaagaaa taatgatttt
    15241 cagcagcttg tttcaatgca tctattgttc cattaccgat aactttttgc tcaaatgctt
    15301 gtttctctgc agccaaaccg acaaaatccg tattgctact cttcaattcc acaccaatac
    15361 ccgtcaccgt aaaatctggt ttttcttcga tcctatactt tgccatttct cccacccttt
    15421 ctattatatt aagttcatct tacataaaat taaagggaaa tgacacgatt acgctctttt
    15481 ttctagggat atttttattt tttaactatt tttttgcttt cactgcaatc caaatctcac
    15541 tgtaaacatt actgaaatcc tctccccatt ttgtaaaaga gatctcggga gcttcgacta
    15601 acccataatc tgaagaagga agccattctg catagatttt cccccaagta tcttgtaaag
    15661 tttggggaaa tggcccttta ttaggaaaaa tggcccaagt gtgttcttct atagcaagca
    15721 tttctaaatc agcgtacgga ttttcttttg tcgtagcaaa gccaatcatg tgcatcaaac
    15781 tgcccttttc ttccaagcgg tctccatcaa atccataaga aacgttgatt acttgatttg
    15841 gatataaatc tcctaactta tgcatctctt ccctttgagt ctccgtgatc gtctgcgcta
    15901 acttttgtat ttcttgattg actccttcaa atttgacagg aacttttttc gccacacctg
    15961 ccacataaaa ctgttctttc ttttcgatac gaaattccat cgattttcct cctctgacat
    16021 caataaaaaa tgtcaagcgt ggaaaagatt tttgtatgcc gtatctactg atttctgaag
    16081 gcagatatcc tgaccaatca cgaaaagctc ttgaaaaacc ttcgactgtt tcatatccat
    16141 atctaaacgc cgtttctgtc actcttgatc catctagaag atcgcgatta gccaaagcca
    16201 atctacggtt tttgatatag tccattagcg acatccctgc aataaacgta aagatgcgtt
    16261 ttaattgata ttcagagatt cctgtatttt cggctattat tttataggaa agtccttctg
    16321 tcaaatgttc ttcgatgtaa tcgatcaatt ggttcagatc tttaagcata tttatcttcc
    16381 tccttgattg cctttttatc atattatcaa agaaagtcaa ttacttgatg aatcaggaac
    16441 caatttagtc agatgtagaa aatgttgtat ggatctaaag aaaattctca agcagcgttt
    16501 cgtaacggat aattggtatt atctgcctat ctatgtttta cctttagaaa aataaatttt
    16561 ggggaagaca gattctaagt aaacaaagcg gtaaaaatat cttttattcc cttagttaat
    16621 ccgcttacta cctactgtac aaagcaaatt gttgttcaaa ctgctgatca gaacgctgct
    16681 tggatttttc tttgcgatac atcatacctc ttttatcaat tggaacaatt atttatttct
    16741 aacaaaatca cttcattttt gtcagataaa taaaaaacaa aaaaagttcc atctttaaaa
    16801 cacgctattc ttttactttt tctcacattc tctaagaaaa gaaaaggaga atgaattttt
    16861 gacaattcga gcaaaagtga ttatcaaata tgtaaaatca gctacttttt tcttaaacgt
    16921 ttctttatac taaatataaa taagtgtttc atgaaaggag aactagatga ataattggct
    16981 tgagtatttt cctgaaaacg ttttggaacg cggttacagc tatcatctgc acggttttgt
    17041 ccgtcatttg aactatacaa gcaagtactt gtccgcaaca gtttctggca cggaagatta
    17101 taaggtagtg atcacttggg atgaaaagac caacatgact tgtgattgtc tatatgccat
    17161 agaaggaaaa aaatgcaaac acatggctgc tgtcttattc gcttacgaag aacgtccaat
    17221 aaaaaaatcc aactactcac tttccgaact ctcgtccctt gtttcctccg ctagttcctc
    17281 tcttgtacgg gagcttttaa cagagattct tatagaacat ccgcacttta tcgaacgctt
    17341 caaagttaag atgccatttc atgcaataaa ttattcagat aaattaacaa ctatcatcca
    17401 taaatacgat catattatca agaaaaacaa aaacaggaaa acagcgaagt tcatcatgga
    17461 gatgcgaaag ttcatccaag aagcagttga atcgcttatt cagcaaaatg cctatcttcc
    17521 cgcattcgaa ttgatcaatg aagtgattgc tactctcgaa acattctatt gggagcctga
    17581 ggatgaacga acccttcttc tgatagagga ctgttattat ttatggaaag aattattagc
    17641 agaagcacct catgcagaaa aaaggcaaat gttctcttgg ttcgtctgcc aagtcgatca
    17701 tactgacgcc tcttattcaa aacgctactc tataaaaatc ctgaaagaag actttcggga
    17761 aaaagaattt tctaaccaaa agaaaaaaat agataaaaaa ctaaagaaac ggtaaagaaa
    17821 gacgatttca atgaaaaata gcaagtaaac gataacaacc atcctaaatc cacaagaaat
    17881 acagcagacc ttgctttaaa aacacgaatc atgcaaagga tgaagtaaag aagacttaac
    17941 gcgtacccaa tattagtcat ttgactatta ggtacgcgtt ttgttttata agtcatgaca
    18001 aatttccttc gttgctggat agattttccg gcattgttga tagattttat gatacttttc
    18061 tacattttgt aagagaggtt ccgctgcatc tgtatattgg ataaaattct gacaacaact
    18121 ttcaggtgtg tcgaaccacc ctaaccctag tgcagcaatc attgttgcgc ctaatgcagg
    18181 tccttcctcc acacttaaac aatagaccgt tgcatcgaaa atatcagctt ggatttgtag
    18241 ccatgcaaga tttttcgcgc ctcctcccac cgaaacaata cgtgtaaatc tgcgacccgc
    18301 tagttcttta cgtttcaatc ccaccattga caaatgctca ttaaaaattt agaatattca
    18361 caagtttaac gacaattatg aaaatttatg tcgtgctaaa ggagttttgt tattggatga
    18421 aaagttagat ataaaaactg cgatcaaaga cacgttacct accgttttcg gttatatcgg
    18481 tattggactt gcatttggta tcgttgggaa agctgccgga tttcatccac tagtcgtcac
    18541 gttgatgtcc ctgctggtct atgctggttc tgcccaattt atcacagtca gcatgcttgc
    18601 tagtcacagc ccattgcttt ccatcgtttt ctcgaccttt ctagtcaatt cccgaatgat
    18661 tctgatgagt atgacgattg ctccttattt caagaaaaat cggctgcttc aaaatctgct
    18721 gattggcaca ttgctgacag atgaaagttt cgcactggga atgaataaat tgaactatac
    18781 tggacaaaag ttaaactttc gttggatgaa tacagctaat tggatttctt atttgacgtg
    18841 ggtgggttct tctcttgttg gcgcactttt agggaatttt attactgatc cgaaaaagtt
    18901 tggtttggat ttcgccatcg tcgctatgtt tatcgggtta ctttatcttc aagtcatttc
    18961 tgatagaaac acctctaaac gattacagtt gatcctgatt ggtctcactt tgattctggt
    19021 atatgtcgga ctgattttta tcccaagcaa cttagtgatt gtagttgtaa cactgattgg
    19081 gtgcggattg ggggtgtgga tcaaacatgc cttcttttga gtttacattg ctgactattt
    19141 taggttgtac tttagcaact tggctttcta gggtcctacc atttgtctta ctgaaaaaat
    19201 tcgatttacc gcaaccgttg cttgagtatt taagttttgt cccgatcgtc attatgtctg
    19261 ctttatggtt cagcagtcta tttacccaaa atatcggaca tctgccgcag atcaatctcg
    19321 aaaatgcttt ggcttcagtt cctacacttt tagctgctat tctttcaaaa agcttattgg
    19381 tgatcgtctt ggcaggaatt ctttcccttt cgttgattcg cttattataa ataaaaaaac
    19441 aagagcttat ggcaaattac tgtcatgcgc tcttgttttt atcaaagtca aataagaaaa
    19501 aaatcggcgt attcttgctt atccgttgaa cggaccacta acaacgactt ttcctaacat
    19561 atgtcctgtt tctacgtctt tggttgcttg tttcaagttc ttcgcattca tcccatctga
    19621 atagaccttg ctcaatgtac tgcggataat cccttcattt gctaaagcag caatgtgcgc
    19681 taaagcttct ccttgcgttt cgatttcata gttatagtct gttttagcga acatatactc
    19741 ccagtcgaag ctgatagatt ggtttttcca aacacctata tctaacggct ctttgatccc
    19801 tacgatcgtt cccacatgtc caaatggtcg gacaaggtgc ttgatttgat caaagtagcg
    19861 ggtaatatcg aataatacag cgacatactc tacttgatcg attcctaatt cacgaaaact
    19921 tttacttaag tcgtgatgat aatcgattgg atagtctaca cctgtttttt tcagccattc
    19981 aaaattcttc ggacttgcag tcgcgtagac ttctaggcca gcccaatgcg ctagttgatt
    20041 caaaatcgaa ccgacgcccc cggatccgtt gatcactaag atttttttcc cgcgattggc
    20101 attttcttct ggagttagac caaatttttc aaataacaat tcataagcgg tcaatgaggt
    20161 caacggcaag gctgcagctt cttcatctga aagatttttt ggtgcaagag cgacaatacg
    20221 ttcgtcaact agttggtatt cttgattgct tcctgctctt gttgttgtac ctgcgtaaaa
    20281 aacgcgatcg ccgacagaaa atttttgcac ttcgtcacct acagcaacaa ctttcccgac
    20341 tccatcaaaa cctaagatac gtaaagcatt tcttatcccg ttatcttggc gaagtttcgt
    20401 atcgacagga ttaacagata ctgcgttgac ttttaccaga agatcccgtg aactgggtac
    20461 aggtatctgt tctttttcat caataaagct ttttggatca tcgatcggca acccttcata
    20521 aaaaccgact accttcatta attccttttc tgacatacga attcctcctt agcttcatca
    20581 agttggagca tccatacagt gttcatttca atatatatag tatgctattt gttccacctt
    20641 ttttattcat cataattatt ctcttactta aagtaaacta tttgcacttt tttggttaaa
    20701 aaagaggttg cgacaagact tttgtcacaa cctcttataa acggtgttct cctaccaact
    20761 cttcggtcac aagtcaaaat attca
//
LOCUS       DACEUZ010000038        20709 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.038, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000038 DACEUZ010000000
VERSION     DACEUZ010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20709
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.038"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            <1..325
                     /gene="sgrA"
                     /locus_tag="IXG44_001205"
     CDS             <1..325
                     /gene="sgrA"
                     /locus_tag="IXG44_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HAQ1657937.1"
                     /translation="SSESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSV
                     LKQPDFVSRGIDHQEKAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSL
                     FHRVK"
     gene            623..979
                     /locus_tag="IXG44_001206"
     CDS             623..979
                     /locus_tag="IXG44_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1657938.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            982..2139
                     /locus_tag="IXG44_001207"
     CDS             982..2139
                     /locus_tag="IXG44_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1657939.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(2165..2605)
                     /locus_tag="IXG44_001208"
     CDS             complement(2165..2605)
                     /locus_tag="IXG44_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAQ1657940.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            2765..3271
                     /locus_tag="IXG44_001209"
     CDS             2765..3271
                     /locus_tag="IXG44_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ1657941.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            3439..5352
                     /locus_tag="IXG44_001210"
     CDS             3439..5352
                     /locus_tag="IXG44_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAQ1657942.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            5357..6358
                     /locus_tag="IXG44_001211"
     CDS             5357..6358
                     /locus_tag="IXG44_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ1657943.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(6560..6766)
                     /locus_tag="IXG44_001212"
     CDS             complement(6560..6766)
                     /locus_tag="IXG44_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HAQ1657944.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(7412..8260)
                     /locus_tag="IXG44_001213"
     CDS             complement(7412..8260)
                     /locus_tag="IXG44_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ1657945.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            8424..9326
                     /gene="gnd"
                     /locus_tag="IXG44_001214"
     CDS             8424..9326
                     /gene="gnd"
                     /locus_tag="IXG44_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HAQ1657946.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            9328..10329
                     /locus_tag="IXG44_001215"
     CDS             9328..10329
                     /locus_tag="IXG44_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAQ1657947.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            10326..10610
                     /locus_tag="IXG44_001216"
     CDS             10326..10610
                     /locus_tag="IXG44_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ1657948.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            10643..11440
                     /locus_tag="IXG44_001217"
     CDS             10643..11440
                     /locus_tag="IXG44_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ1657949.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNAAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            11454..12287
                     /locus_tag="IXG44_001218"
     CDS             11454..12287
                     /locus_tag="IXG44_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1657950.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            12305..13162
                     /locus_tag="IXG44_001219"
     CDS             12305..13162
                     /locus_tag="IXG44_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ1657951.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLMQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            13184..13693
                     /locus_tag="IXG44_001220"
     CDS             13184..13693
                     /locus_tag="IXG44_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1657952.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            13730..14131
                     /locus_tag="IXG44_001221"
     CDS             13730..14131
                     /locus_tag="IXG44_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1657953.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            14243..14596
                     /locus_tag="IXG44_001222"
     CDS             14243..14596
                     /locus_tag="IXG44_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1657954.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            14630..15073
                     /locus_tag="IXG44_001223"
     CDS             14630..15073
                     /locus_tag="IXG44_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAQ1657955.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            15090..16175
                     /gene="uxuA"
                     /locus_tag="IXG44_001224"
     CDS             15090..16175
                     /gene="uxuA"
                     /locus_tag="IXG44_001224"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAQ1657956.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            16168..16881
                     /locus_tag="IXG44_001225"
     CDS             16168..16881
                     /locus_tag="IXG44_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ1657957.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            17633..17833
                     /locus_tag="IXG44_001226"
     CDS             17633..17833
                     /locus_tag="IXG44_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ1657958.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
     gene            18401..18964
                     /locus_tag="IXG44_001227"
     CDS             18401..18964
                     /locus_tag="IXG44_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1657959.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
     gene            complement(19025..19390)
                     /locus_tag="IXG44_001228"
     CDS             complement(19025..19390)
                     /locus_tag="IXG44_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1657960.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(19466..19711)
                     /locus_tag="IXG44_001229"
     CDS             complement(19466..19711)
                     /locus_tag="IXG44_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1657961.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(19781..20035)
                     /locus_tag="IXG44_001230"
     CDS             complement(19781..20035)
                     /locus_tag="IXG44_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1657962.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(20199..20522)
                     /locus_tag="IXG44_001231"
     CDS             complement(20199..20522)
                     /locus_tag="IXG44_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1657963.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
BASE COUNT         7306 a         3203 c         4226 g         5974 t
ORIGIN      
        1 gagtagtgag agtagcacag gaagcacgag cagcgaaagc agcacaggaa aagtaaataa
       61 tgaacttgat ttggtaccta tcataccaac taaggaacaa aagagaaatg cagggagcaa
      121 cctaaaaagt gtgctaaaac agcctgattt tgtttctcgt ggaattgatc atcaagaaaa
      181 agcgaaagaa acagataata gaaaaagcga tttaccagaa actggtagta gaaccttgaa
      241 caagctaatt acatggatgg gtgtgttact cattttgatt gtaggagcaa gctattttcg
      301 ttctctcttc catcgagtga aataaagaat aaaagcaaca gatctaaaat tgaataaaac
      361 gatagatttt tgagtgaata tttcattcga gagtgctgta ttgcctggcg aagttgtagc
      421 tttgccagat ctatttttgt ttactaacat atttgagaaa caggaaataa caggaacaca
      481 aatggaaaat aagagcaaga ataattgaca atgcacactg cgcatggtag tatctctgaa
      541 aaatcagtaa cgaatctatt tgtacgttat tcatgtagct gtcggaataa gtctaaataa
      601 tagataaaaa ggagtatgta ctttgtttga agaatttatt gatatcaatg aaaggcaagt
      661 atatcaattt ttgaactatt gttatgaaag agacgagaaa ttatatgtgg tgaaggatat
      721 cgctttagat ctaaactaca ccctggctaa gatgaattcg gtgattcaac aagcagaatc
      781 gttttgcgaa aggtatccag aatataaatt atcttttctt tcagaaaaca agatgataaa
      841 agtcgagttt tcttctcagt ttttactgag taaagtttat tctatacttt tagagggaac
      901 gattggttat attttactgg attcacttta caaaggtact tatcaatcct tagaaaatct
      961 tagtcaaaaa attatataag tttgcgtacg attcaaagaa aattaagaga acttaatact
     1021 attttgaaaa actatcattt atcttgcagc ttgaaaaaag gtaatccgat agaaggagaa
     1081 gaataccgta tccgatactt ttttcacttg atgtattggc agtcattcga cgaaaatcat
     1141 tctgatatat tatcaagtat aaaaaataaa cgtcaactat tagaaaaatt tgcgaaacat
     1201 gcgccatata cgcgtagtat agatagaaag aaatggatca gcctactaag aatttcgttg
     1261 caacgaatga aacaaggcca ctcgattacg tatgtcccag aagagatgaa caaatttgtg
     1321 catccattga taacaaaaga agagtttacg cattacatac tagagccttt ttttgaagca
     1381 aacttctggt cgatgaatca catcactcca acagagcaag cttaccttta ttttatgttt
     1441 ggtgtaatga acacctattt ggaagaagat ttgttggata ctccagataa agtacaagaa
     1501 agttggggct ggattgaatc gtctattgta gaaaattttg aaaaaaactt caagatttgt
     1561 tttacaactt cagaaaaaaa ttatctatat gtcaatttgg caatgatcca tttgtattca
     1621 agggtttttg ggacaaaaaa gaaggtggat gcttttggaa aatcagcaga agacagagat
     1681 tttcagcata tatttccagt gatgtatcca atcatgcaac agttttttag tggggtagct
     1741 cgcagaattc cagagcttaa tacttattat gaaaagaaca gacggttgat ttttcaatac
     1801 tgtatgttag tcagggatat ttttataaaa cacgaacaac ctgtcatatt ctatattcaa
     1861 tcaaagtatg gtaaaacaca ggaattatgg gcaaagaaaa ggatattatc tatgactgaa
     1921 cgcccaataa aatatagtgc atccgctgat cagttacctg atattgtcat tagcgattat
     1981 cctatcgaca aaaaggcata tgatcaaacg aatacacaga tattttactg gaatggacaa
     2041 cctacaaaaa atgattgggc gcgtctgaaa gctgaaatca taaaaatcag gaatgagaaa
     2101 aatagatcca tattggaaaa attggatttc gttaattaag aaaagcagat tgtttatgcc
     2161 aaaattaaga cattgcaaaa agattatttt ctttaaaatc gacaagatga ccagaaagca
     2221 agagttcgtt gagctcttgc tttcttttag ttttagaaag gaacatcctg attttatctt
     2281 cgctaaatcc agtcaaaagt tttgcagatt ggtcgtagat aatcggtttt ttcaataaag
     2341 aaggggatgt acgaatctca atatatagtt cactcaaact gagttcatct attctgtgaa
     2401 caagattttt ataactaata gaatgagtag agagcatttc ggtcacgccg ttgtttgtca
     2461 attttagtaa gtggataaat tcatttttgg ttagtggttg acgaattaag ttctgttcga
     2521 taaacggtat atcatggacg ataagccaac gttttgcgcg tcttgaagac gagcagccag
     2581 aacctgtata caatttaatc atcatacatt cctccaatag ctaagtattt atgaaataat
     2641 aacatgaaga aatagtcgaa aagtgccatt tagaggtgta aaataagaaa atgttgtcac
     2701 gtataatgga actagctaga taagaaatac tattaaatca agaaaaaaga aagaaggcac
     2761 aagtatgtca aacacagccg acgcaatagt agtgatcgat ttacaaaaag gggtatgttt
     2821 tgattcttcg ccgatacacg aattagaaac agtgacagca ttagtgaata aaagaatcaa
     2881 aacatacgaa acgattggga aaccagttat tttcgttcaa cacgaagatg ctcaattagt
     2941 gccacaaaca aaaccatggg agctattgtc ggaattatct attcctgagc acagttattt
     3001 tgtgagaaaa acccatgcga attcatttta taagactaat ttgagtgttt tattaactga
     3061 attggactgt cattcgatcg aattatgtgg ggcacaagca gagtattgta tagatacgac
     3121 tgtgaagttt gctcatggat taggatacca gcttatgatg tgtagaggag caacgacaac
     3181 ttatgataat ccttttatga aagcaaagga aacaatcgct ttttacgaga aaatttggga
     3241 taaacgattt ctgacatttt tggagcaata agttgtttct tcgtttaaaa aaagcagaat
     3301 ggatactggt ttatccattc tgctttatta cgtaaaatga gaaaataatc ctgttctatt
     3361 tttatgggat aaaccattta ttttttgtat aaaaaatgtt tgcgtttaca tataattctc
     3421 ctagaaacat aaaaaggagt gaatagcttg agaacaaaac aagtattaaa gggagcgttg
     3481 gtcatcttat ttgctgtaag tacaagcgtc ccgcaatgga cagtatatgg agagagcatt
     3541 tcttccgaat cggaatctcg ggaggtagaa accacagaaa ctactgatgc tggaaatgaa
     3601 acgaaatcga gtacttcaca aacagaaaca aatactgaaa caacggttga gacatctgtt
     3661 actagcacag atcaagaaac gaccgctacc acagataacc aacaaacaga agaagcaaca
     3721 gctacaaaaa tgtcaaacga cgaatcctct tctgctaaag taagtgacaa agagaatggg
     3781 acagaaaatg atgatatcgg taaaggtgaa aatttcaatg gtaaagaata tttgaattac
     3841 ttcggtaaac agatgacaga aatacttaat ggaccaacag aagacatcaa gtcaaaatta
     3901 atagagttat tagtcactga gcttgctgtt aatgattatg gtgtagataa agagattatc
     3961 aaacatctga ttgctactta tctattgtac gatatcgctt ttgcaaaaaa cagtgtgttg
     4021 atgtcgatgc ttggaggtac agatgatggg acaacgccta cagaaaacac aggcttctat
     4081 ccaaatacaa aacaacttca tcaaactgta ctaaacaaac aagatggcaa aatgatggat
     4141 gtatatgcat attatgtcga tcaaggttca gataaaacag tcatgatcca tggcggattt
     4201 cgagggaatt ggaataacgg gatcgttaca gaagaataca atgattttta taaggccggg
     4261 tataatttat tatttgttga ttccagagcg actggtaata gcggaggcga ttatgtgacc
     4321 tatggacaat atgagtcaga tgacgtcctt tactggataa atcaagaagt tcgagagcgg
     4381 ccatctcaaa agattttact ttatggtggt tcgatgggag cagctacgat gatgtctgta
     4441 ttagcgaaag atattcctgt caatgtcaaa ggaattattg aaaattgcgg atttgcatca
     4501 attgatgaac aactacgttt tacgtacagc cagacagtag cccccgcact accggatgcc
     4561 ataaaaaatc agttagacat cattggggat caagagcatg aagatttatt tatgggcctt
     4621 ttgaaacaat actattttga tcaggagatg catttggata caaaagctgc tctacctacg
     4681 attggaatgt cagattcatt gcctaaattg attatccatg gaacagcaga tgatgtagta
     4741 cctgtgtcta acgcgcaaaa attatatgag ttatcaggag ggtataaaga tcttttatta
     4801 gtagaaggag cagggcatgg aaaggcacag gaagttgacc atgctgccta cacgaaacat
     4861 gtaacggact ttttgaaagt agtgttccat gatcagataa acgtaaagta tgtggatgaa
     4921 aacaataaaa gtctactcaa agaccaggac gaaatccagc tttacggagc ttatggagaa
     4981 aattatatga cagagcaaaa gacattcgaa ggatacgaat tagcaaatgt tgaaggaccg
     5041 actgaaggga tttttaatga gacgatacca actattattt tctattacaa aaaaatccca
     5101 gtagctccac caaaaaagca agatccgaca gagaatgcag acaataaggg aaagaatgaa
     5161 cctcgagagg atcaatcaag tgatgatacg caaccgattc agatgacagt gaaagtacaa
     5221 aaaaagacag atgcaaaaaa aagaatgttg ccgaaaactg gagaaaagaa acattcgctt
     5281 ctcatttatt caggtggatt tttatctgtt agcacgatta tcacatggat ttggcgaaga
     5341 aagagagggt aaatgaatga aatggttcaa gcgaatagtg attgcagtac cagttatcat
     5401 cgtagttatt ttagtcactg tatttttgat taatcaaatc acaccaaagc cgattagctt
     5461 gcttgttaga aaacaatttg acacaagtga aaaagaacaa gtatatgccc gaccagatga
     5521 tttccagcaa aaaatcgaac aaatcgatat aaaaaaagaa atatcctatc cttcaaagta
     5581 caaaaataat aatatggata tctacacacc gaaagtggaa aagaaaaaat taccgattct
     5641 tttctggatg catggaggcg catatgtagg aggggataaa aatgattgtc gtgattattt
     5701 ggaatatcta tgttcagaca cgcagcaaat catcgtaaat atagattatg aacgatcacc
     5761 ggaagcaaaa catcctacac cagttatcca attgaacgaa gccattcaag cagctaaaaa
     5821 agagataaaa gatcaagcag attggtcgaa tatttcgatt ggtggaaatt cggcaggtgc
     5881 acaaattagt ggagaatatt tgttagcact tcaaaatgaa gaaattaaaa aagctgatca
     5941 tcttgatccg actttggaga acaagcagat tacgaagttt atttctttgt ctggtctgtt
     6001 ggatccttct gcacttactc aagtaagtga taaaatctct agctttcttt atgaaaaatg
     6061 tggatgggct tactttgata ataaggactt tgaacaatca gagaaggtca aaagtttagc
     6121 cttggtcaga catgcggatc gttggacgca aaacacattc ttaacggatg gaaatacaaa
     6181 tacgtttacg aaacaaatgg aagaagtaac ttctgtattt gagaaagcgg gggtgaaagt
     6241 cacaaaagta gattatgcgg agaaaaatgc tgttttgaac catgaatatc agtttgattt
     6301 cagcaataaa caagcgcaag aaacatatca aaagttagta atgtttttca aggagtaaag
     6361 tcttatgatg caaaagaggt tgtaatctgt cacttagctc caagtcacaa ggagcaattt
     6421 caataaacaa gcttttcatg gtttatgaat attgtaactt gtgactgagg agttagagat
     6481 ggcaaggggc accttttatt cgaaatttgg gaatatcaca cgattattgt cacgatctct
     6541 tttacgtaaa ttttataagt cagatttgtt gttttgatcg tttaaaaaaa gcaacaaatc
     6601 cgaagcctgc ggaggcaaga agggacaaaa agccgagtgg taagcaaaag aatgctggtt
     6661 ccactaatat tccgttgtta ttgatataag gctgggaaca aagatgaata gcaaataaaa
     6721 tcaatgcact agcgaaaaaa agcaaagaaa tatggatagc tttcataagc agcacctcct
     6781 tttttataat tgtatgataa atagattaag aatctataaa tgtgacaagt ttgttaggat
     6841 gtgaataaaa tgatcatcta gcgaacttga aacaaagatg gctaatgata agcaattaat
     6901 gattcagcaa tcagataggt acaaagttta ccatcatacg tctagaccaa taatagaaaa
     6961 tatgggaata taatagaaaa gatttaggat acccccaaaa gaatttcccc aaaaattcga
     7021 tgtatcctaa acttttttat tttacataac aatcatatca actgtgaaat aaaattacaa
     7081 atgataacct aaaatgaggc caaaagccag tgttatcaag gaaatactta attcaccaaa
     7141 atttttttta attttttaga aaataagaaa caaaaataat aaaaaaaaga aaagataaat
     7201 tttttactac tgtatactaa ctttgttatc gcatctaaca agtaactcga ataataccaa
     7261 tacaaccttc tccaaagatg cgatgacata cgttctaacc caccgactat tgatgttctg
     7321 taccaccctt gcagaaccaa aaaagccact accaaatatt tggtagtggc tttttttgtt
     7381 ttatggattt ttcgcttttt ctgttatggt gctattcttt gatttcagaa atagtatgtc
     7441 gaactttagc gatagaacga cgtgattctt tttcgttgta aaacatcaaa atcacatata
     7501 gagagtccaa aatgcttagt tgagaaatac gggaagctaa agcttcagat cggaattctg
     7561 tttcttcaga aatagaaata aaaacgacat cgccaagttt agcaagggaa gaattagctt
     7621 ggctagtaat aacgattgtt tttgctccag tttttcttac ggtttcagca atatggattg
     7681 tttcttttga ttgccctgtg tgagaaatca aaacagcaca gtctttttcc gtcaataaag
     7741 aagcttccat caattgaata tggtaatccg tgctgtgaga agttgcgata ggggaacgca
     7801 aaaatttatg ataagcgtca gttgctacaa tctctgaacc gccaacaccg aaaaaataga
     7861 cacgtttaga ttcattgata atagtagccg ctttttttag atccttttgt tgtaacaact
     7921 tttttgtgtc atttaaagtt gaaatactag actcgaacac cttttgcgcc atagtgagct
     7981 cagtatctgt tttctgaata ttttcgtgga tagaaatagc agatagatca ttttcttgga
     8041 tcaacatggc cattttaaaa tctttaaatc cattataacc aagttttttt gtaaattgaa
     8101 aaaaagtaga gtcagcaatg cccagttcac ttgctaaatt gctaatagga ctatgactta
     8161 ctttgcttgg attttctaaa atataatcgg cgatagcctg ttcttttggg ctgaagtcgt
     8221 tgtacattgt tttgatttgt agtcttatgg agacgctcat gagaattcct cattttcata
     8281 ataagattat cattttttcc gttctcatta atgttaaaga tacaaggaga gtaggaaaaa
     8341 atcaaacaaa aaatattttt gtaaaatcta tttacaaaaa aatttttttg tataatgagg
     8401 gtataagaaa tggaggcgct atcatggaaa ttggtgtaat tggtttggga aaaatgggat
     8461 tgaatttagc tttaaactta aaagagcacc atcacaaagt acaaggactc gacatttcaa
     8521 gtacagctgt tagtcaggca agagaaaatg acattcctgc ttatcaagag atctctgaat
     8581 ttctagctac ttttgagcag aaaaaaatca tctggttaat gcttcctgct ggagagataa
     8641 ccgaaaacat gttaagaaaa ctttttccaa aactcggacc aggagacatt attattgaag
     8701 gcggaaactc ttattataaa gattcaatcc gtcgagcaga agagtttcag aaaaatgaaa
     8761 tcggttactt ggattgtggg acttctggag gaatcgaagg agcacgacat caagcctgct
     8821 taatgattgg aggagataaa gaaacttttc tatccgtgga acaattattc aaagatgtag
     8881 caattgaaag aggatatctg tatgctggtg catccggaag cggacacttc ttgaaaatga
     8941 tccataatgg aatcgaatat ggaatgatgc aagcaatcgg tgaaggattc caacttgtac
     9001 aaaaaagtga gtatgctttt gacttggaac aagtcgcacg tgtttggaat catggttcgg
     9061 tgattcgcgg ctggctgatg gaaatcatgg aagatcagtt aaacgaacat cccaatttaa
     9121 gtaattatcg gggaatcgtt tctgcatcag gtgaagcaaa atggacggtt gaaacagcgc
     9181 tagaaatggc tgttccagtt ccagtaattg caactagttt gttcatgagg aatctttcac
     9241 aggaagaaga tagtttttca gcaaaagtag tttcagcact aagaaatggg tttggcggac
     9301 atgaaatagt tgaaaagggt gagtaaaatg ccagattatc atatagcaat cgttaattcc
     9361 agcagtttcg gaagagtctt tccacaccac ttggaaagat taaagtcagt tggagacgtt
     9421 catttcttta ctgttgatca agagattcca ggaaaagaac tagcggagtt acttaaagga
     9481 ttcaatatca ttattgcaag tgttacgcca ttttttcgac gagatttttt tgaaaacaaa
     9541 gatgaactgc tattgatttc ccgacatggt attggttaca acaatattga tttaaaagca
     9601 gcaaaagaac accagacaat cgtatctatc atacctgctt tagttgaacg aaatgctgta
     9661 gcagaaaata atataactaa tttgttgtcc atgcttcgac gcacgacaca agcacaagca
     9721 agagtcaagg acgatcgatg ggaagatcgg gcggagttcg tcggtaggac gctgtttaac
     9781 aaaacagttg gtgtgattgg tgcaggaaat accggaagcg gagtaatcga aattttgcgt
     9841 aacggttttc gctgtgatgt tctagcttat gatccttaca aatcagattt atacatcgaa
     9901 agctttggtg caaaaaaagt agaacttcaa gaattactgg aacaatcaga tatcatttgt
     9961 ttatgtgcga atttgacaga agagaactat cacatgattt ctgataaaga agtcgatcag
    10021 atgaaagaaa atgtttatct atctaatagt gcaagaggcg ctctagttga tgaagaagcg
    10081 atgattaatg gtctgaaaag cggaaagatc gcaggatatg ccacagatgt tttagaagaa
    10141 gaaccgggac gtcaagatca tccgtattta gcgtttgaac aagtagtgat tacgccacat
    10201 atatcggcct acacaaggga atgtttagaa gcaatgggtg aaaaatgtgt cagagatgtc
    10261 gaacagatca gtcagcacct tcttcctgaa cgttctgtcc agacaaagag caactatata
    10321 aaaggatgat gaagaaaatg gtgaacggtt taaaagtgaa aacaggccct caattttatt
    10381 tatatgaaga aggtggtatt tcaaaagttt ctgatctctt gaaatcatac ggcgcgaaaa
    10441 gagtactggt cacccacgga acggtatcat gggaaaaggc gctacctaaa ttagtatttt
    10501 tgaatgacga aacgattcaa tttttttatc atcgttattc tggagaatgt agttatgctg
    10561 aagccagacg aatagctaca atcatcaaaa aaatgaaatc gatttcgtag ttggcgttgg
    10621 cggaggaaaa ctgaccgatc tagtgggtta tagtgccaat cttgcaagaa tcgatttcgg
    10681 agtcatccct actttagcta gtaattgtgc tccatggaca cctcttgctg tcatgtataa
    10741 ggagaatgga caagcagaag ggaaaacaga acacttttac agacaagctg ctttttttat
    10801 aacagatccc caattagtga ttgattcccc tgttccatat ttcattgccg ggttagcaga
    10861 tacactagca aaatggtatg aatctgagct catcttagaa caagattttt tgcaagatga
    10921 atcatttttg aagctagcac aagatactgc gaagatctgc aaagaagaga ttttgaatca
    10981 ttctgctaaa gcaatccaag atatggcaca aagaaaactg acatcggagt tcaaacagct
    11041 ttcagagatt atctttgctg ttgctggttt agttggagga ttaggagata aatacgcaag
    11101 aaatgcggca gcacatgcta tgcatgacgc tatcagtaaa tatattccgg aaagccatcg
    11161 ttttctacat ggagaaaaag tagcgtatgg gattttctat caactggcac tagaaggtcg
    11221 ctgggaaaaa atagacgagt tatatccttt gtatcgggaa ttagaactgc cggtttctct
    11281 agaacaaatg ggattatttc cgaaagataa acagataata gataaaatgg ctgaatttat
    11341 tgattcgaaa gagaaagtgc atttgattcc tatagaggta acacagaaat cactgataca
    11401 gtcaatgatg gaactggaag gttatatcag aaagatctga aggaggagga cacatggaaa
    11461 cattaagtgt agtacaaagt ttgttgattg ctttatgggt agccgcggtt atgtcccgct
    11521 ggttaggtgg aggagccacg ttgaccttac gtttttctcc tttgatgact ggattagtcg
    11581 tcgggttgat catgggggaa gttgcacaag cgatgattgt tacagccgct ttgcaaatga
    11641 tttatatggg ggttttttct ccaggaggat caatgccagc tgaaccatct attgcagcag
    11701 ctatcgcagt accagtcgca ttattagggg atctgtcgcc agaagcagcg attgccgttg
    11761 cggtgccagt cggattgctc ggaagttact tgtatcagtt tcgttttttc atcaatactt
    11821 ttttaggtaa atatacagac aaagctgtag aagatttgaa tacaaaagct atggcccgat
    11881 caattattct ttatccaacg gttgcctcat ttttattatt cgttccgctt gttttcattg
    11941 ctttatattt aggggcgcca ttgatcgctg atgtaatcaa agctttagag ggaacagtcg
    12001 ttatccacat tcttgaagta gtaggtggag ggttagcggc aatcggtatc gcgacaactg
    12061 tatacgttat cggtcgaaaa gattatctgg tctttttctt ccttgcttat tttatgagtg
    12121 ttgttttgaa atctttagat attacgatgg ttacttatgc catttttggg attattatcg
    12181 ctttgatctt tgtccagtcg caaaaaggga aacaagtaca gcctgccaca agcaataaca
    12241 attcagcatc ttttgatgac gaagatgact atgatgacgg attttaattt aggaggaatg
    12301 ggaaatggct gaaataatgg ataatcaaac aggtgctcct gaagaaatca cgaaaaaaga
    12361 tgtaacaaaa gcctatctga gatggcattt tgcaaacgaa attcctcact cttttgaacg
    12421 ttacttggcg ccatcactac tatacggaat gatgccaatc ttgaaaaaac tttataaaga
    12481 tgaagactct ttaaaagcag catataaacg gcagttactg ttcttcaaca ctcaattaag
    12541 ctggggtggc ggtgtgatta ctggcctaat gtcttctatg gaacagcaaa gagcagaaga
    12601 agagtatgaa gaaaaagaga tcttgatgca ggatgacttg atgtataaca cgaaagcggg
    12661 attgatgggg gcactagcag gaatcggaga tgcaatcgat tcaggaaccg tccaatatat
    12721 ttttatcgca atcgcggtac cttgggcaca acagggaagt cctttaggcg cactttttcc
    12781 ttttatctgc tttgggttgt atcaggtttt actaggggta tttttcgcaa gacaagcttt
    12841 cactatgggc cgtaatgcta caggcctaat gcaaaacact ggtgttcaga cagcgattga
    12901 aatgctatca gttttagggt tgtttatgat gggggtgctt gcgggaaact atgtaaaagt
    12961 agaatcttca ttgaaatttg cgatatccgg acgtgagttt gttatccagg aaattcttga
    13021 tcaaatcgtg ccagggatat tacctttagc tgtcgtaatg ggtgtgtact tgttctatac
    13081 gaaaaaagga ttgaaagtca cacaagcatt attatggctg actggaatat taattgtgct
    13141 agcaggaatc ggtattttat aaaaagaagg gaacgtgaat aagatgggtg tagtcaattt
    13201 agcgcgtgtg gatgagcgtt tgatccatgg gcaagtaatg gtaacgttat ctcaaaaaag
    13261 tggagtgaat tccatttttg ttgtagacga agttgtagca aaagataaat ttatgagaga
    13321 tctttacaaa aatgcaggaa gccgtacagg acaaaaaaca attgtcatta caccagagaa
    13381 agctaaattc tattgggatg aatatcaatt taaagaatac aattgtattt tgatcgcaaa
    13441 aacagtccaa acgatttacg acttagtaaa acacggaatc cccatggaag aattgaatat
    13501 tggagggatt gctcaaaaaa atcctgatac agatctttta gtgacgaaat ctgtttattt
    13561 aaataaatca gatgctgaaa aattgaaaga aatgaatgaa caatatggcg taaagaatat
    13621 ttattttcaa gccactccat ctgcacctaa aacaggatta aaagatgtat tgaacaagtt
    13681 tgatttgtca taaacaaatg cttagaaaaa agataaagag ggcgaaaaaa tgaagaagaa
    13741 cgagctgttt cgtgattggg aatttcgtta tcgctatatt taccgtaagc gccgtacaaa
    13801 aaagagtaaa cagcgttttc tttcagcatt agtatcagat atttacagta tgagaacaga
    13861 tgtgacagtc attgcttacg atacgctagc ctaccgatca aaaaatattt atgttggaga
    13921 tatcgaaaaa gcagagaaag ttatctgtac ttattacgat acacccgttc atgcattagg
    13981 aagttatttc atgtttgatt ggaaagacca aaggaaaaaa acaatctact ctatattatt
    14041 gtccttcatt ttgttgttca gtttaggttg gtggggaatg atgatatata acaaaaatcc
    14101 tcatcacgta ttcgatctgc tatccgtata aacaggtatc actgtactag catttgggag
    14161 ctactttttc ttattgggaa aagcagctcg cggatggcct agcagacaaa cttttattcg
    14221 aaatacttca tccatattaa cgatgctaga aatgatccgt acgattgatg atccgagtgt
    14281 agcgtatgcg tttgttgatg aaggctgtta tggagaaaag ggcttagaca gcgtgagatc
    14341 gggtatgaaa aaagaggcta ttctttttta tctagactct gttggggcag atacaccgct
    14401 acagttttcg ggaaattatt tttcaaataa agaacagtgg ctgaaacaag ttgataagtt
    14461 gaaagaaaaa aacgtcaatt atatttttag tgcaagaaaa aagcaagcgc agttcttcta
    14521 tttgaccaaa acagatttac gagggaaaac atttaattgg caaaatgcca atcagattat
    14581 tgctcttttt cgataagtaa aaagtgcaaa acaaggagtg agaagactaa tgttggaaat
    14641 catgatcgtt acacatggta agttgagcga aggactgaaa gatgcggcgg aagtaattgt
    14701 cgggacatcg agccatattg aaacaatgtg cttagagtct ggggaggaca tccaaacatt
    14761 cggagagaag gtcaaagaaa aattaactat gatcaacaca gcagaaccca tgctagtatt
    14821 tgttgatcta attagcgcaa gtccgtacaa tcaagcgctc ttggcagtca atgaactgac
    14881 tgaagaaaag aaagcagcca cttatgtcat agggggcgtg aatctgccaa tgttgttaga
    14941 agcaatcaat catcagatgc ttcacacacc gatagaagaa gcggtggaac aaattgtcaa
    15001 acaaggaaaa gataatttaa ctgtttggca tgcttcagcg gaagaaaaaa aattagatga
    15061 ggacgatttt tagaaggaag gctgaaaaga tgaagtgggg atttagatgg tatggtgaaa
    15121 aaggggattc gatcccatta aaatatgttc gccaaatacc gggaataaat gggataattg
    15181 gaactttgct gactaaaatg cctggagatg tttgggagat ttcagaaatt gaggagttga
    15241 aagcatctgt tgagagccaa ggactggaac tgcttggaat tgaaagtgta gcgatccacg
    15301 atgcaatcaa ggcagggaca gatgaacgag attactatat tgataactat atacgaacaa
    15361 tcaaaaactt gtctcgatgt ggggttcgat tgatttgtta tagcttcaaa ccaatttttg
    15421 gatgggcgaa aactgatttg tttaatgaaa acaacgatgg aagctattct cttttatacg
    15481 atcagaaaat cgtcgatcag atggagccag gagaaatgta tcaattgata cacagccagt
    15541 caaagggatt caaattgccg ggttgggaag aagaacggtt gaaaaaattc gctcagctgg
    15601 aaaaactgta tgaaggaatg accgaagaag aattatttga aaatctgact tattttttag
    15661 aaaaagtgat tccagtgtgc gaaaaagcag atgtaaaaat ggctatccat ccagatgatc
    15721 cgccatggga actttttggt tttcctagag tgacgaaaaa tcttggcgac ttgaagaaaa
    15781 ttctaaatgc agtagattct ccatataatg gactaacgct gtgcacagga tctttagggg
    15841 caaatccgga aaacgattta gttgaaatca tccatgaggt aggttcgcgc atccattttg
    15901 tccacttcag aaacgtggag tttttaggag aaagaaagtt caaagaaacg gcacatccct
    15961 ctacagaagg ctcccttgat atgtatcata ttatgaaagc gttggtgcaa gaagggttcg
    16021 acggtcctat acgccctgat catggtagaa ccgtgtgggg tgaagtggct atgcctggat
    16081 atggcctgta tgatcgtgca atgggactca cttacatgca aggtctatat gaggcgataa
    16141 gtaaggaaaa accactggga gagcattatg aataaaatca agctcttcat ctttgatatg
    16201 gatggccttt tattagaaac tggacgttta gcttatcgtg cttatgtaaa atcagcacaa
    16261 aagtatgatt atgaaatgcg taaggaagtc tattatttac taactggcca aacagaaatg
    16321 gcgataagag aacaaatggg cattttgtac ggagaagatg ttccatatat caaatggcga
    16381 gaagcaatca atcaatataa agaaaaaatc gtgaaagaag ataagagagt atatacgaaa
    16441 aaaggagcag aagaaatttt atcttttgca aaagaaagag gaatccatac aatcgtggct
    16501 tcgtctaata cacgggtaaa agtagaaatg tatttaaaaa tggaaaatct atatgccttg
    16561 tttgatcaaa tcatttcagg ggatgatgta aaaaaaggaa agccggaacc agagatcttt
    16621 ttgaaagctt gttcaaaaat gaatattcct ccaagtgaag cacttgtatt tgaagactcg
    16681 attgcaggga tcgaagcagc aaggcgagcg ggcattttgt cttttctcat caaagatcat
    16741 ttgatagggt tgcccgatca taatgggaag cataagttga aagtagatgt aagtttatat
    16801 gaatccaata aagcagacta tgaattttat gacctgcatc aagcaaaacg atttttagaa
    16861 agtaaagggt tgtatctcta ataaagagga ctattttcta gtcctcttta tttattgtcc
    16921 tgcacgactg agggaacaga tgaaaaagaa gtaatttttt attttgaagt atctacctta
    16981 tttgtagtga acaatttaga acaaacacaa aacgcatcta ctgttatcta ccagtagatg
    17041 cgtttaaaga gaagaacgag aatcgtccat atgagggttt ccactcctta tgcagatttt
    17101 tttaattctt gcactgcttg ttcgatcgta acacctgtcg ctacatgatt ggtattttct
    17161 ttgtcaaaaa cgacaaatag attcaaatct gtatccaagg ttactcgata ttgtatatct
    17221 ttgttataga atgtcatagc cattctcctt tctttaaata cttgctgaaa ggcaacgaac
    17281 gcgttgataa cgaaattcca tttataagta taccaccaat tgaaaggttt tcatagttaa
    17341 gaaattttaa agtcaaaagt tgcagataaa agcaaataaa caagaaaaca aaaagtgaat
    17401 caatcgcggt tattcaactt tttcttcgat tcgaagacaa tagaagacaa tgaattttat
    17461 atttcattgc attatccttg tgttattaga aaaagcatga tatattaaat gagtaatcta
    17521 ttttgaaacg taatttgagc gatatatcta catagataaa actccttttg cctatcaaat
    17581 atttattgca acgaaaaaac gtacattgat acgtatcagg aggaatttat ttatgaataa
    17641 cggtacagta aaatggttca acgcagacaa aggttttggc tttatcactg gtgaagatgg
    17701 aaatgatgta tttgcacatt tttcagctat ccaaggtgac ggcttcaaaa ctttagaaga
    17761 aggccaagca gtgtcttacg atgttgaaga tggtcaacgt ggccctcaag caacaaacat
    17821 tgttaaagct taataaaaat tgacttttaa ccgtcttttc gaaagaaaag acggtttttt
    17881 tgtacgaaaa aattcattgg tcgttgaatg ggctgaatag tagtgtataa aacaattttg
    17941 tggagcgtga agcttttatg gtagaggaaa atacgaataa agtgatgttg ataaaaagat
    18001 tcctatcttg aatatgaatc gagatgtctt gaaaatgaat gagtttgacc aatcattatc
    18061 ctatcgaaat ttacagtttc gttcgattgg tctgtcaatt aatgaaaaag taaaaaagaa
    18121 tatgttctag gaagagggcg gcacatctaa atttttaagt aacggacagt tcaaatatca
    18181 agcattgaaa gaggctatcc tactgtttga aggaatacga atatataaaa acgacaaaga
    18241 ggaaaaagta gttcattaaa tcccttcaag tgatgaaaga atgagatcta ataaaacctg
    18301 atctttcatc tgttttttta ataacgtaat aattttttta ttttttgtgc tttcgccgtt
    18361 attgtagagt gatttagcat tcttattttt aaaaataact gtgttatata ttaaaaaagg
    18421 cataatagtt ataagctttt ttaaagaata tttagaaatt attaattttc gtctgaaaac
    18481 aaaaaaagaa agcgttatta ttttagtcta ttttcagggg gggaacctta tgataaaaaa
    18541 actattttta ctatcaatat taacgctagc aatggcaggt tgttctgact ctggcttagc
    18601 aactgaactg atcacaaaat caactgaagc cacgacagag gagttaaaat gtggaaacga
    18661 tcacagtgac tacagaggct taaatcgacg atgcatggga atattaggca gaagcaattg
    18721 taagcatcga tgtagtaatc gaagaaggtg tagaagaagc aacagaaaaa tcaaaagaaa
    18781 gatcaaaaga agcaatctgt gctgtgcatc gtatacagac gtcgaagatg cggtactttt
    18841 aaaccacgtc aggagatgtt catttatgtc tagggaagtt ggcgatgaac caaaactaga
    18901 gtacgctttt agttttgcac gtgaggatga agataaccct aaaacttacg acattttagc
    18961 ttgatctaag atggttaaag ctttttaatg aagtaagagg agatagaagg cttttctagt
    19021 tatattaggt aagataaatt cctttttgct ccaaatttgc taaacattct gctagataaa
    19081 cttcatcgtg ttccggataa atcggcacgt tgcgctctac agtaggtaaa tcaaaaaatg
    19141 cttcgcatga tttgccacga tacataggat ttttccactc tggtgctggg ctgtaaccac
    19201 gtttttccat ttcatccatg acaagctgat gatattggaa taatctataa ggagaatagt
    19261 gaaaaacata atccacagta gcatgtttct ttccccagcc atttcctctt aatgcacagc
    19321 attcccgatg ttgtcctaaa agctgttgac gaggaagctt tgaaatcaat ttttcatgcc
    19381 ataatcgcat agaaatgtct cctttatata tagattgtta accttagacg ttaaatgaaa
    19441 aaaccagcac caaggctgga aaaaatcact tcagtttgct tgctgtttct gagcgaccaa
    19501 ttaggtaatc aacggaaata tcgtaaaagt cagcgatata gattaattca atgatgcttg
    19561 gaatttcttc tccttcttcg tacttacgat atttgaataa aggaatactt aacgcgctag
    19621 aaacagctac ttgagataaa gatcggccag ttcttaagaa attcagacga caacgtaaag
    19681 tatccagtaa aatagcttct tcgatactca tttaatcacc tttcattata gtagaatagt
    19741 tataatgact atagaaagct acacatgcaa tcaatagtac tcaatgtttt ttatttaaac
    19801 gtatcaaata aagaatgacg atcaataaag acaaaaccag tcctgataat tcttctataa
    19861 aaacactatc gggttcaaag tagttgtaat taaaaacttt ccacataaag tagatagccg
    19921 gaagagtcaa aatagctaaa taaaaccgtt ctttgttcca attcataaga taaacaatac
    19981 ttgcgaccag tgcgaccgca ggcacccaaa acaaaactct caccaacgta atcatgtaac
    20041 ggactcctca acgattcata taataaaaga taacatgtta ttgcatacat cacaaatgaa
    20101 atgctaggaa aataaaagaa gaatagatca atgaaaaatg aaaaagcaga acagttttct
    20161 gctgataagc aggaactgtt ccgctgaatg tgtcttatct atcgagaaaa aatcaatgga
    20221 ttgatatcta agtaattcaa tacatataaa aagaggacga tcgcattcac taaaaaggaa
    20281 agaatgatga tcccatagcg aatggaaaca tatttaacaa atacttttgt ttgaatacgg
    20341 gcatatcttt ttcctttata gctgagcatc atgacaagaa gcaaaagggt aatcacacct
    20401 tgaaaaatca ttccaataaa cagaattcct gaggtttgat aatttaattg gagaaaataa
    20461 gctgctgccg caactaaatc gaataaaatc gcaataaatg gcaaaataat cactcctaac
    20521 attgagaata aataaaagaa taacattttt tctaaaataa gcaaataaaa attaaagtaa
    20581 caaaatagtt atgacacaag taaaagaaat aagaataaca atattatttt acttatattt
    20641 tgaaacttgt gagagtttgt cattcgtgct atagttgaaa agtaacaaaa ataaatttta
    20701 tttatgatg
//
LOCUS       DACEUZ010000039        20660 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.039, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000039 DACEUZ010000000
VERSION     DACEUZ010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20660
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.039"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(3..1991)
                     /gene="treP"
                     /locus_tag="IXG44_001232"
     CDS             complement(3..1991)
                     /gene="treP"
                     /locus_tag="IXG44_001232"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HAQ1657964.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLVYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            2214..2927
                     /gene="treR"
                     /locus_tag="IXG44_001233"
     CDS             2214..2927
                     /gene="treR"
                     /locus_tag="IXG44_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HAQ1657965.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            3004..3450
                     /gene="msrB"
                     /locus_tag="IXG44_001234"
     CDS             3004..3450
                     /gene="msrB"
                     /locus_tag="IXG44_001234"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HAQ1657966.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            3620..4222
                     /gene="ruvA"
                     /locus_tag="IXG44_001235"
     CDS             3620..4222
                     /gene="ruvA"
                     /locus_tag="IXG44_001235"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HAQ1657967.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            4235..5236
                     /gene="ruvB"
                     /locus_tag="IXG44_001236"
     CDS             4235..5236
                     /gene="ruvB"
                     /locus_tag="IXG44_001236"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HAQ1657968.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            5265..5843
                     /locus_tag="IXG44_001237"
     CDS             5265..5843
                     /locus_tag="IXG44_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAQ1657969.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKVLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            5895..6377
                     /locus_tag="IXG44_001238"
     CDS             5895..6377
                     /locus_tag="IXG44_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HAQ1657970.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIAIEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            6494..6868
                     /locus_tag="IXG44_001239"
     CDS             6494..6868
                     /locus_tag="IXG44_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1657971.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
     gene            complement(7668..9020)
                     /gene="brnQ"
                     /locus_tag="IXG44_001240"
     CDS             complement(7668..9020)
                     /gene="brnQ"
                     /locus_tag="IXG44_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAQ1657972.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
     gene            9167..9757
                     /locus_tag="IXG44_001241"
     CDS             9167..9757
                     /locus_tag="IXG44_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAQ1657973.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            complement(9885..11234)
                     /locus_tag="IXG44_001242"
     CDS             complement(9885..11234)
                     /locus_tag="IXG44_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1657974.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(11402..12142)
                     /locus_tag="IXG44_001243"
     CDS             complement(11402..12142)
                     /locus_tag="IXG44_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ1657975.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            complement(12155..13747)
                     /locus_tag="IXG44_001244"
     CDS             complement(12155..13747)
                     /locus_tag="IXG44_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750719.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ1657976.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            complement(14315..14611)
                     /locus_tag="IXG44_001245"
     CDS             complement(14315..14611)
                     /locus_tag="IXG44_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1657977.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            14787..15512
                     /locus_tag="IXG44_001246"
     CDS             14787..15512
                     /locus_tag="IXG44_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HAQ1657978.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEMAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            15505..16347
                     /locus_tag="IXG44_001247"
     CDS             15505..16347
                     /locus_tag="IXG44_001247"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HAQ1657979.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKAVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            16350..16871
                     /locus_tag="IXG44_001248"
     CDS             16350..16871
                     /locus_tag="IXG44_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HAQ1657980.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(17124..17687)
                     /locus_tag="IXG44_001249"
     CDS             complement(17124..17687)
                     /locus_tag="IXG44_001249"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAQ1657981.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            17938..18207
                     /gene="rpsO"
                     /locus_tag="IXG44_001250"
     CDS             17938..18207
                     /gene="rpsO"
                     /locus_tag="IXG44_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HAQ1657982.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            18395..20521
                     /gene="pnp"
                     /locus_tag="IXG44_001251"
     CDS             18395..20521
                     /gene="pnp"
                     /locus_tag="IXG44_001251"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HAQ1657983.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
BASE COUNT         6747 a         3972 c         4174 g         5767 t
ORIGIN      
        1 gactaagatt ttacttcagc tttaccgact gcttcgcccg ggcgaacaag tcctgattgg
       61 tcaagagaaa gtcctgcaac tttttcatta ttggtcaaag caacaataat gtcagttttc
      121 ttgccagctt gttctatttc tggcaaatcc atttcagcaa tcagggtctc tggtgtgaca
      181 aggtatcctt ctttcacgaa tacattgaaa ggaacaccct ttagttcaac agtatctaat
      241 cccatgtgaa caagtacttc cagcccctca ttagaaagaa taccaatggc atgttttgtt
      301 tcaaagacac tcgtcacttt accgtttact ggtgcataga ctttgccgtt tgacggttcg
      361 acagcataac cgtcacccat cattttttgt gagaaaactg ggtcatttac ttcagttatt
      421 tccttgatgg tccctgcagc gactgcgaat aatgtttctt ttgttgcaac tgccgtacca
      481 gttgctgaaa caggttcgaa agactgtggc gttgctgttg cgccatttgc tgtttgaagt
      541 tgtacatcag cagcattttc aggaacagca ggatcaatct tattcaaaat tccctgacgt
      601 ctaaagatga tcgtcaagcc aaatggaatg atgacagcaa tgatcattgc gatgatgaaa
      661 ggtacccatg tttctgcttg gatagctagt attcctggca aaccgcctac tccgatcgcg
      721 ttagctcgaa cacccatcaa gttagcaaac atgccagcta atccagagcc gaccattgca
      781 gcgacgaaag gatagacgta tttcaaattg attccgaaca tagcaggttc agttacgcct
      841 aagtaacaag aaatcattgc tggaatcgag atttgttctt cttttttatt cccacgatgt
      901 aagaagatga tggctaagac tgcagagcct tgggcgatat ttgataaagc aatcatcggc
      961 cataaattcg ttgatccgaa atctgcaatc agctgcatat cgatggcatt ggtcatatgg
     1021 tggagaccag taatcactaa aggagcatac aggaatccga atacggcact aaacaaccag
     1081 ctgatagaag acgtcaatcc agtatttacg atatttgaga tccagcttcc gactgtccag
     1141 ccgataggac ctaaaataac gtgtgcagct aaaacagttg gtaacaatga aaacaatgga
     1201 acaaaaatca tcgagatcga ctgagggata tatttgcgga agaagatttc tagatacact
     1261 aacataaacc ctgccagcat ggctggaata acttgcgctt gataaccaat catctggact
     1321 tgagcaaaac caaaatccca aaatggaata tctgctgcgg ctgtggaagc tacactataa
     1381 gcatttaata attgcggaga aactaacgta attcctaaaa cgattcctaa tatttgtgta
     1441 gtacccatct tttttgcaat actccaagtg atacctactg gaagaaaatg gaaaattgct
     1501 tcacccggta accacaaaaa ggcattgaca ccattccaaa acttcgagtg ttcaacgatt
     1561 gttccaccaa gactttcaaa ctggatacct tcaagtacat ttctgaatcc aaggatcaat
     1621 ccaccgacaa tgattgcagg aatcagtggt gtaaagattt ctgctaaaac ggcaatagca
     1681 cgttgaacgg gatgaaggtt ttgttttgca gctgcttttc cttgttcttt ggaaacccct
     1741 tcaactccag atactgcaac gaagtcgtta taaaaagtag aaacatcgtt tcctatgatg
     1801 acttggaact gtcctgcatt cgtgaacatc ccttttacgc taggtatatc ttcgatcgct
     1861 ttttcgtccg cttttcctgg atcattcagg acaaaacgca tacgagtcgc acaatgacta
     1921 actgcagcaa tattttcttt tccgccgata tcatgtaaca gcttctcggc atctgcttga
     1981 tattttgcca tatcaataaa cccctttctc ttaacttgtc tatgcaagtt gtttcttcat
     2041 ttactatact ttttttcgga gagagttgca agcgttttat taaaaagtac gagagaaaat
     2101 gatatcgttt taaaaagctt aaaataagct tttcttaaaa ataggaacat tgaaatacaa
     2161 aaagagctgt agtatagtga agaaatgatg gaagaacgag tgagggaaaa aacatgaata
     2221 agttcaatga aattttttta gatttggaac agaaaatttt agcgaaagaa tatcctccgc
     2281 attcgctttt acccagtgag aaccaattga tcaaaattta tggtgtttca cgagaaacaa
     2341 ttagaaaagc gctgaatctt ttgactacgg aaggttatat tcaaaaaaaa caaggaaaag
     2401 gttcgatcgt actggatcgg aatcgcttcg actttcctat ttctggattg actagctata
     2461 aagagctaca ggagacccaa cgtattccaa gtgaaacgat catccatacg ttagaagaaa
     2521 cagaagtgac aaaagcattg aacgagatca ctggctggga gatcggtgcg cctgtttggc
     2581 atttgatacg cgaaagaaaa atcaatggcg aagtagttat attagacacc gattatctat
     2641 taaaggaaat cgttcctatt ttaactccag cacaagcaca aggatcgatc tatgaatatt
     2701 ttgaaaatga gctttctttg acgattgctt acgcgcaaaa ggagatcaca gtagaagaag
     2761 taacggaaat catgaaaaca gcgatggatc ttcacgatga cacttatgca gtagtagtca
     2821 gaagcctcgt tcatctggaa gatacccgct gtttcgagta ttctgagtcg gttcaccgat
     2881 tagataaatt ccgtttcgtt gagtttgccc gacgccgaaa agtgtaaata gttcaaaaga
     2941 aagcaaattg ttggcatctg tccactttgg cgctatgata ggatcacaaa gggggcattt
     3001 ttaatggata aaccaataag accagaaaaa gaagaactga aagaaaagtt atcacccatg
     3061 gcttatgcag tcacacaaga aaatgcaaca gaacgaccat ttagcgggaa atatgatgat
     3121 ttttacgaaa aggggatcta tgtagatatc gtcagcggtg aaccgttatt cagctctgca
     3181 gaaaaatatg atgcaggttg tggctggcca tcattcagca aaccaataac gaaaagacaa
     3241 gtgcgcgaaa aagccgattt ttctcatggc atgcatcgaa tagaagttcg aagcaaagaa
     3301 gcagattctc atctcggaca tgtgtttacc gatggaccag tagatcaagg tggtctacgt
     3361 tactgcatca attctgcctc actgaaattc attccatatg aaaagatgga tgaattaggt
     3421 tatggggaat tcaaatcact tgtagaataa tggaaataca caaagagcgg tacaatacgg
     3481 ggatttactc gatcgtccgc tcttttttta ttcaagcgat tgctatttga aagaaacgct
     3541 ccccagactc cttttttcac aaggtgaaat gtgctacaat aaagggaacg tatgtgtgca
     3601 aaagaaagag gactagaata tgtatgaata tctgacaggt gtggttacct ttatcaaccc
     3661 ctattacata gtagtagaaa ccaatggtat cggctaccag atcgcactag gaaatcctta
     3721 ccgctacagt agcaaactaa atcaaaaaat aaaaatttat gtccatcagg taatacgtga
     3781 agatgcgcat cttctgtatg gatttgattc gctagaagaa aagcaactgt ttttgcgatt
     3841 agtaagtgtt tcaggaatcg gtccgaaaag tgcccttgca atcatggcaa gcgaagacca
     3901 ctccggtttg attcaagcag tagaatccag tgatgtcact tatttgacga aatttccagg
     3961 agtagggaaa aagacagcac aacaaatgat cttggatcta aaaggaaaat tcggtgagct
     4021 gagtatcgat acacctttta gtctatttga tgaagcaaac acaaaagacg ctacagctct
     4081 atctgaagca atggaagctc tctcggctct tggttacagt gatcgagaaa tcaaacgtgt
     4141 agaaaaacaa ttaaaagaag tagataatca gacaacggat gagtatctac gccaagcact
     4201 caagctaatg atgaaaaaat aaggaggaga ccaaatgaca gatgaatact tgttatcacc
     4261 agaatctggc gaaaatgaac tgagtttgga aaaatctctt cgaccacagc tgctttctca
     4321 atatatcgga cagagaaaag tcaaacaaga acttcagatt tacatcgaag ctgccagaaa
     4381 tcgggaagaa cctttagatc atgttctgtt gtacgggcct cctggattag gaaaaacgac
     4441 gatggccatg gtcattgcta atgagatgac cgtaaatatt cgtacgacga gtggccctgc
     4501 tatcgaacgt ccgggtgact tagtagcaat cttgaatgaa ctggaagcag gggatgtcct
     4561 gtttatcgat gagatccatc gattacctag agtggtagaa gagatgcttt attcagctat
     4621 ggaagatttc tatgtagata tcatggtagg acaagggcca accgcacatc cggttcattt
     4681 ccctcttcct ccctttactt tgattggcgc gacgacacgg gcaggtatgc tttctgcacc
     4741 tttacgagat cgatttggca tcatttctca tatggaatat tatgacgaac aggatctgca
     4801 ggagatcgtt ttacgttctt cggatatttt tcaaacagag atcattgaag aaggggcatt
     4861 ggaaattgct cgccgatcac gaggaacgcc tagaatcgct aatcgcttgc tcaaacgggt
     4921 tcgtgatttt gcgcaagtcc aaggagacgg gaaaatcgat cgtcctatag cggatcaggc
     4981 acttactttg ctacaggtcg atcaggctgg attggattat gttgaccaga aattgttgcg
     5041 aacaatgatc gagttatatg gcggcggacc agtagggtta agtacgcttt cagtaaacat
     5101 aggagaagag cgtgagacgg tagaagatat gtatgaaccg tatctgatcc aaaaaggatt
     5161 tttaaagcgt acacctagag ggcgaattgc tactgcttat gcctatgaac attttggtta
     5221 cgactatcat atatgatttt tgcttttaga ttggaggaaa atgaatgagt gatacaacac
     5281 gtacgaaaca attgatcaaa aacgtattga ttgatttgtg ccaaacgcaa tcttttcgaa
     5341 aaatcagcgt ccaaaaaatc gcaaatgaag caaaaatcaa tcgacaaaca ttttattatc
     5401 attttacaga taaatacgat ttgcttcgtt gggtctattg tgaggatgca ttgcattatt
     5461 tagatccaca atgtctttca cttgataatt gggaagagca agcattgaaa gtgctgaaag
     5521 caattgctga gaagaaagaa ttttatgcaa atactgtgct ttctgaccca gaaattttac
     5581 aaaaagagtt ttctcaactg atccaaaaat catttatcaa gatttttgaa caaatggatg
     5641 aagaaagaca actttctgca agagacaaac tattttatgc tcgttttttt tcttacggtt
     5701 gcagtggtgt attagtcaat tggatcacga atgaatatga agaaacaccg atggaaatcg
     5761 ctatgcagct tttccgttta gccaaggata cggaattata ttcttatcgt ctttatgcaa
     5821 aagaagagtt ggaagaacag taatagaaaa attttttgtt ggaaaagcaa taatcgctaa
     5881 aaggagatgg ggaaatgaca aaaattttat ttgtttgttt gggaaatatt tgccgttcac
     5941 cgatggcaga aggcttgctg agaaaacgaa tagcaataga aggaagggaa gcagaatttt
     6001 ttgtggattc tgctgctaca agcacgtatg aagttggaaa gacaccgcac cctggaacga
     6061 aaaaaatatt aaaccaagaa aaagtagata tgacgaatat ggttgctcgg caaatcacac
     6121 cccacgattt tgaaactttt gattggatca ttggcatgga tcaagaaaat gtagaagaac
     6181 tgaaacgaag agcaccacgc tcagcgcagg ggaaaatcca tttgttttta agtagcattc
     6241 ctggaaaaga aaaagaaaat gttcccgacc catactatac caataacttt gatgagacat
     6301 atcacttgat cacagaaggc ctgaatcatt ggtggaatat ttggattgac gaaaataaaa
     6361 taacaaacaa tttgtaaagt tgacgctttc tttttaaggc gatgttagag catttccttg
     6421 tttttttgta aaatgttagg taaaataaaa ttaattaagt aacactttct tagctaaagg
     6481 aggagtgcat tcgatgaacg aagagacagt atcgccaatg aagaagctta cgaagttagt
     6541 tactcatact tcgtatcaac tttttgattc ttttgtaaat gacactgaag aacatgctaa
     6601 gaatgtacga ttgcaaggaa agtttattct tgagctgcaa aaagctgcta ctgaacatag
     6661 tctagttatc ttacaagtaa gagcgaatcg tttgagtagc aagtatgaaa cgatctcagg
     6721 ctgggtggcg acaaaaacgg tcgaaaaaga ccgagtgatg atccgcctac aaaacgaaga
     6781 gcagcagatc cggatggttc ctgtagaaca aattgaaaaa ataacaatcc tttcaccagc
     6841 aggggaacag gaacgattat caaaataatt tataccagat agaaaagcac gtatgagctt
     6901 gtaccatcaa tcattactaa tcaaaaataa ccgaatgctt taagctgata tcagtaaaac
     6961 ggaacgtttt aggatgagta tcacagcatg acagcattgg gttgattagt tgaatcgatt
     7021 ggatggtaca agtttttttg ataaaaaaag gtcctgctag ccggggagct agcaggataa
     7081 ggagttaaaa atgaaaaagt gttgttaggg ttgtttgttg gtatgcttat ataatagtca
     7141 ctagttgtga atattttgtg acgaaagttt tcgaaagttg ttaagttttt ttgcgaaaac
     7201 tatttttata tcatatatat gtctaaaatc tatattataa aagaaatgtt gtttgatcag
     7261 cttctcttcg gtattagttc aaaacaaaac ggcaaagtat atggagctac tttgccgttt
     7321 tgtttctcta atactcaggt cagtttatag acgctcaata aacggtgttc aaaagccagt
     7381 ttctcggtat taagtcaaat ttatgcaaaa atgtgagaag ccattttcac aaaatatttc
     7441 ttaatacttg aaactaagcg gctttttcac gacctctcaa taaacggtgt tcaaaagtca
     7501 gtttctcggt attaagtcaa atttatgcaa aaatgtgaga agccattttc aaaaaatatt
     7561 tcttaatact cgaaactgag cggctttttc acgacctctc aataaacagt gttcaagaga
     7621 cagcctatcg gcaataaggc tgaacgtctc tttcacaacc tcttttatca atctgctact
     7681 tcttctttct tagcgaaact ccaaatcagt ccgataacaa aaccgataac tgctggtacg
     7741 atccacccca tccccaattt gaaaaacggc aagtagcttt ccgcaacata taaaagattt
     7801 tgaacaatag gtgtttgttt gatagtttct gggcaagcgt tcaacccatc aaaaatcgaa
     7861 gcaatcaatg taaaataagt agtcattcga taaacagccg tacgctgctt aaacaaagga
     7921 ccaactaaca ctagtaaaat cagtgtcatt gctaatggat agatgaacat caaaacaggc
     7981 aacgaaaatt gaatgatatt cgtcaagcct acgtttgccg ctaaacaagg aaggatacta
     8041 gcaagtgcaa caaagaatgc gtaatttctt tgcggaaaca aatctgtgaa tgtttctgca
     8101 aaagctgtaa tcaaaccaat accggtcttc aagcaagcta atataacgat aaatgctaat
     8161 aagatactgc cataagtacc taaataatga tcagcaatct gagccagcgt gatcccgcca
     8221 ttttcactta gagcaaagct acctaagctc atagcaccta aataagcgag caaagtataa
     8281 atgattccca tcaatataat gctgatcgca ccagatttga tcgtgtcttt agcgatttca
     8341 gcaggttttg ttacacccat gttttggatt gttgtgacaa tgatgatccc aaatgctaaa
     8401 gcagcaagcg catctaatgt gttatatcct tgagtaaacc ctgtgaaaaa agcatgctct
     8461 tgataatttg gctgaatagg cgcttgattt acagaaccta gtggattcaa aaaagcaagg
     8521 agaagcaaag cacctaataa aaccaaaaat gcaggattca ggaactttcc aacataatct
     8581 aatatctttg tcggttttct tgaaaaccac caagccagca caaaaaatac aatactgaaa
     8641 attgctagat atagcgtctg tctgctagga tcgataaatg gtgctaaacc aatctcgaaa
     8701 gatgtggttg caaggcgagg taaagcaaaa aacggtccga taactaagta aagcaagatg
     8761 gtaaaaacca tcgcatatgt ttgatttatc cgttttgcta attcatatac acctgagctt
     8821 ttcgatacac caatggcaat cacccctaag aagggaagac cgataccggt aaccaagaaa
     8881 cctagattcg cccaaaaggt agcagcgcct gcttcttgac ccaaatgaac gggaaagata
     8941 agatttccag caccaaaaaa tagaccgaaa agcatggagc ctataaaaag atagtcttta
     9001 aatgataatt tttttgtcat acataactcc tttacggttt ttttaaaagt aacaaaattt
     9061 cgaaaaatat gcaacaattt tcagaaaatt tcctatataa aacgacggtt atttaataac
     9121 gtaagtgata aaataaaagc gctgaatcat ctgaggagga atcatcgtga caaagtcgag
     9181 attagaagca tttactgatg gagtggtagc cattgttttg agagttcttg tattagagat
     9241 caagatccct aatacatcta gctttcattc tttatgggtc atccgaaata cattattagc
     9301 ttataccatc agctttatat ttgttgcggt gatctgggtg aaccatcatc gtatatttca
     9361 gatggcagaa cgaattaact accgagtggt ctggtcgaat atctttttgc tcttttggct
     9421 gactttatgt ccttctgtta ctagttgggt cgggcgtaac cctgaagctt tttggcctga
     9481 gatggcttat gtcttagtat acacgatgtg gagtttttct tttggtatct tgatgaggca
     9541 aatcatgaaa gcaaataaac cagattccca tgtcgtgaga gttttgtcaa aggaccgtcg
     9601 aagtcttatc tccatgatga ttaacttatg cttgattgcc ggtgtctttt ttgtaccgtt
     9661 gatcgggtta ttcggacgtt tttttgtatc tggtatttgg attttttcct ataaaaaagc
     9721 agatgcctat tatcagcggt tgtttccggg gaaatagaaa agaggctggg acagaagctt
     9781 ttaactccaa gaaataagcc gaaatttcac aaaaatttaa gaagcaattt tcgtaaattc
     9841 cttcttattt ctcgaagctg accacttctg tcacaactac tttcttactt cttacgttca
     9901 tatttcatat gatagattag taatagaagc aagaaccaca caggtgttag taataaagcg
     9961 attctcgtat cttttcctag gaacaagacg acacacacaa aggccatgta aagaaatacc
    10021 agataattac taaatgggta caacggcatt ttaaatgaat gagctttttt gccgaggata
    10081 cttttacgat atttcatgtg cgtgtagaca agcatactcc agataaacaa gaaacaagta
    10141 gtggcaatac tgcttactag tgtgaatact tcactaggca tcacgtaatt caagataaca
    10201 gaaatgaaaa tgacgatagt agaaaatgtc aaagcattcc ctggcacatg atgtgttgtt
    10261 aactttttga attttttagg ggcactgcct tcttgcgcta aagaacgaag catacgtcct
    10321 gtactatata ttgcactgtt gcaggcagaa gctgctgcac ttaatacgac taaattgatc
    10381 aaggaagcag caatcgtgat cccaatttcc gaaaatactt ctacaaatgg actgttatct
    10441 ggattcaagc tgttccatgg atagattgcc ataatgacaa aaagagatcc taagtaaaat
    10501 aagatgatcc tgataggaat attattgata gcttccggca acactttttc aggattttct
    10561 gtttctccag cgactaatcc aactaattct atcccagcaa aagcaaaggt taccatttgg
    10621 aaagctaaga taaatccttt tgcaccagtt ggaaagaatc cgccatgaga ccatagattc
    10681 gtgatactgg ctgtgccaat atctgtcgta tagtgggtga ggatcatata agctcctacg
    10741 aggataaagg caatgatcgc tacaactttg atcaatgcaa accaaaactc taactcacca
    10801 aacaatgaga cagccaccat attcaagcaa agaagaagac ctaatgcaat cagttcagga
    10861 agccactgag gaacaccagg cgcccagtag cgtacataca ttccgatggc tgttaaatcg
    10921 gccattgcaa tcgataccca gcagaaccaa tacgtccagc cagtgatgaa agccgctttt
    10981 ttgccaagat attctgcaac aaaatccaga aaagaatggt tattggtgtt tgataacaga
    11041 agttcgccta acgcacgcat aatgaaaaaa cagatgcatc cagtaataag ataggcgagc
    11101 aatatcgaag ggccagctag ctggatagac tttccggaac ctaaaaacaa ccccgttcca
    11161 atcgctccgc caatagaaat cagttggaca tgtctatttt tcagtccacg ctgcagttct
    11221 tgctcttcat tcatttttaa atcatcctaa ctaaaaaagt ttaattactt ccatattaaa
    11281 agagttttct gaaaaacaca actaagattc caaaattttc agaaatttac cttttatgag
    11341 taattcataa aaaagaactg cggtaaacac cgcagctctt tgtcccaatc agctattgat
    11401 cctaagcatt taaaatacgt gaaaggaatt cttttgtacg ttcttctttc ggatggacaa
    11461 agatatcttc tggtgtacct tcttctgcaa tcacaccttt atccataaaa atgacacggt
    11521 cagatacatc ttttgcaaat tccatctcat gcgtcacgat aagcattgtc aagccagtat
    11581 gtgctaagtc tttgatcgta ttaagcactt cgccaaccat ttcagggtct aacgccgaag
    11641 taggttcgtc gaatagcaat acatctggat tcattgataa tgcccgagca attgcgacac
    11701 gctgtttctg tccaccagat aattgtgctg gtttggcttc gatataacga tccataccaa
    11761 ctttttccag attttcgatt gctacttttt tggcagtttc tttatctcgt ttcaggacag
    11821 ttgtttgacc agacatacag ttttccaaga cattcatatt gttgaacagg ttgaaggatt
    11881 ggaaaaccat acctagattc gttcgatatt taggtaaatt atatttagga gctaatacat
    11941 tttctccatt ataaatgatc tcgccgcctg ttggtttttc cagtaaattg atacagcgca
    12001 agaaagtaga tttaccagaa ccggaagaac cgatgatcgt cacaacttca cctttattga
    12061 ctgttacgct gatatctttc aatacttcat tttcaccgaa gctttttctt agatgtttga
    12121 tttcaataat tttttcattc atgattcttc ctccttattc ctgcaaattt gaattgtcga
    12181 tttcttcaag cttggtataa gctgaaggac catcgatttt cttttctacc gcccgcagga
    12241 ttctggtgat cgtaaaggtc atgactaagt agatgatccc gacgatcgta aatgtttgga
    12301 agaattggaa attcgcaccg gaagcagagt ttccttggaa gaacaaatca gctacgccga
    12361 ttacacttaa aactgctgta tctttgatat tgataacaaa ctcatttcct gttgctggca
    12421 agatattccg taaaacttgt ggaataacaa cttttctcat cgtttggcca tgggtcatac
    12481 caatggcttg cgcagcttcg aactgccctt catctactgc aaaaatcccc ccacgaacga
    12541 tctctgtcat gtaagctcca gtattgagag agacaataaa caaagcggca atcgttcggt
    12601 tcaatgaaat gccaaaagcg agtgccagac cgtagaagat gaccattgct tgtaccatca
    12661 tcggtgttcc gcggaatact tcgacataga tagaaagaag tgcatttccg attttttgga
    12721 agaagcgagt cagtgcattt cctgattcag gtaccgaacg gaagacacca atcagtaatc
    12781 caagtgtagt accgacaact gtcccgatca atgcgatgaa caaggtaagt ccagcacctc
    12841 gtaagaacat attgccgtat tgtttccaaa tcgtagtgaa atcagaaatg atgccaggtt
    12901 tttcttcact atcagttgca gcaggctgat ctttgactgc ttggtccata attttggttc
    12961 gttcatcgtt agagatgcca gaaagaatct ggtttacttt ttggatatcc gggtcgcctt
    13021 tacgcatacc aacagaaatc tgaacatctt ccgggtttgt ctggaagcca tctgcaccgc
    13081 tgaattcaag catttccaag tctttgttca cactagttgc cgttacacct tccggacgtt
    13141 cgctgacata accatcgatc attccagaag ctaaagctgt tctcattgct gagaaattat
    13201 ccattgcttg ttgtttctgc acatctggta tctgatcgat cacagtatag tggaatgtat
    13261 ttaactgagc agtaatcttt gctccagaaa gatctgctaa actttttgct tttgcatatt
    13321 tgctatcttt ttgaactact actactaatt gtgattcgta ataaggatcg gtaaaatcaa
    13381 tttcttttcg tcgttcagca gtagggctca ttccggcgat gatcgcgtcg atcttgcctg
    13441 attgaagggc aggagccaga ccatcccatt tcgtttggac gatgactaat tttttcccta
    13501 aaccatctgc tactttttta gcgatttgaa catcataacc gccggcgtaa ctattttggc
    13561 cttgaattgg caaggcatta ttctcatctg tttgttgaga ccagttgaag ggtgcatacc
    13621 cagcctccat acctacgcga aactcatcgc tcggtgtttt ttcatctgca tttgccagag
    13681 atcccacact gaatagtgag aagacaaaag caaatagtac ggcaactgaa accatatttt
    13741 ttttcatttt tttctccctt tctgattata tgatacacga gcggtctaga tgaaatgcgg
    13801 taaagcttta tctgaaaaaa taaaaagcga ccgatttata gatataaaaa ggccgctaaa
    13861 attccataag gtgataaacc tctcacaaaa catagcgcaa cttagcatca ctgctaagac
    13921 agtccgcaag ctcttaactt gcgtcccaac atacactcca agcaaaaaag tgcacatttc
    13981 ggcgatcctc cctattcctg cttctccatg tttgcttcac tccacagggt taatgattct
    14041 cgcgacctct acctcattga ttgaggcatc tcgtattcag ttatttatac aaaaaaagtt
    14101 tacgatactc gagaggcttt gtcaatctat aactgctgtt ttttgaaaaa atactgataa
    14161 atatagaaat ctttactttt accgaaaaaa caatcaaaat gtgaatcatc tattcattgc
    14221 aaacgatagt ataattatac acaaaaatgt atgtaataca ataaaaaaag aactagaaca
    14281 gaaaatttcc tgtgtccagt tccatctcga tcatttaagc tgccgctttc tttggctcac
    14341 gtcctaggat cgcttgcaaa agaacaacga cgccataaat ggctaatatt agataaatca
    14401 aacttgagta ttgggtgatt ccccagcccc atgttcgata tacggctaat gtaatggcta
    14461 aaacaattgc ggcaatcacg ttgaataaag caaaagccca aagatttcca ttttctttgc
    14521 gtacaagata aaaaacagag aaataaacgc ttaacgctaa ccaggcgata caagccagaa
    14581 taacgcccca ataaataaag aatggtaaca tatcgattca cctcactttt atacagcagt
    14641 attgtaacac gttttcattc ttttgtcagt gaacattgtg ataaattgtt caaatgccca
    14701 taacactttt ttattcccat ttttctgact tttcaacaat aaatttgttc gagtatgggc
    14761 tataatggaa agtgaggtga caaaacatga aacaattaac agtagacgaa gcagcaaaag
    14821 agatattagc ttatcagcaa attacatttt tgaccggtgc aggggtatcc acaccttctg
    14881 gagtgccaga ctatcgatct ttgtcaggaa tctaccacgg gatggagcag ccggaatact
    14941 tgctgagtca tcaagcaatg gtgaatgaac cggataaatt ctatcaattt gtcaaaaaga
    15001 tttatcatcc tgaagcaaaa cccaatatca ttcatttgga aatggcacat ctagcgaaag
    15061 aaaaaaatgt ttgggttgtt tcccaaaaca tcgacggttt gcacgcaaag gctaaaagcc
    15121 cgcaactcgt caattttcat ggaagtttgt atcaatgtca ttgtcgaaaa tgtcaacaaa
    15181 cggtagactg gaaagaatat cttcacagcg acaaacatca agcatgcgga ggacaaatac
    15241 gcccagacat cgtgttatat ggtgaagggt ttcaagatgg agtcatggag caggcggctt
    15301 atgccgtcag ccaagcagag ctgatcgtca ttgttggaac gagttttcaa gttcatccgt
    15361 tttgtgatct ggtacagttt cgccagccgt cagccaaaat cttagtaatc aatcaaacgc
    15421 ctgtttattt agcggagcct tatacgttta ttcaagaaga cggagcggtt gttttcaaaa
    15481 aaatacaaga attaggagca gattatgact gaattcaaaa ccgaaaaaga aaaaatgatt
    15541 gcaggcgagc tttattttgc caatgatcct gagctggttg ccgaccgtat gtttgcaaga
    15601 agtcaaagcc aaatcatcaa ccaagcagaa tcagcggaac tgcgatccca attgttgaaa
    15661 gagacttttg gccgtacagg gaaaaaaatc tatatggagc cagttatcaa ttttgattac
    15721 ggatataata tatttgttgg ggagaatttt tatgcaaatt tcaattgtac atttttagat
    15781 gtcagcacga ttgaaatcgg tgataactgc atgttcgctc cgaatgtcca gctttacacg
    15841 gcgacgcatc cactacatcc agtgaaacgt aacagcggtc tggagtacgc aaaacccatt
    15901 aaaatcggta ataatgtttg gttaggcgga ggagttatcg tcacgccggg tgtcactcta
    15961 ggggacaacg tagtagtcgg cgcaggttcg gttgtgacca aatcttttcc cgacaatgta
    16021 gtgattgcag gaaatcctgc tcgtatcatc aaggcagtag aggaagaact aacagaagag
    16081 tctcttgaaa cattacgtca taagatcgat atgatcgatc gagagttggt tgctttatta
    16141 gaaaaaagaa tggatacggt aactaaaata ggacaagtga aaaaagaaga aaaccaagca
    16201 gtttatgatg caaaaagaga gcagcaagta ttggataaag ttgttagtct gctgaaaaat
    16261 aagggatata aggaaacaat aacagatacg tatgttgatt tgatgaaaca ctcgcgcgaa
    16321 taccaaaaca aaatgaagga ggaataagtg tgatcgaagg agagacacgg cgaaaacaaa
    16381 tcatgcaaac cttggcaaat acagaaaaac cagtgagtgc tagcaagttt gcgaaaactt
    16441 ttggagtcag ccgtcagatc attgtaggcg atattgcttt actacgagct gcaggtaaaa
    16501 gtatcgtggc aacagcaaga ggttatatcc tcgaaacaga agaaaccaat actgggcaca
    16561 tcagtaaaat cgctgtccag catggaaaag atcaaacaga agaagaactg agactaatcg
    16621 ttgaaaatgg tggtgaaatc attgatgtaa ttgtggaaca tcccctttac ggtgagttag
    16681 caggaacttt gcacatcaaa acgccacaag atatcgattc ctttatgaga cgttatcaaa
    16741 aaagtaatgc tactttgtta tccgaattaa caggcggcat ccatctgcat acgatccgct
    16801 acccagaaaa gcatatgctg aaaaacatca aacaaaaact agctgaagca ggaatcctct
    16861 acgaaggata acatcgtagc tgctactttt tatcctagaa actattctat catctctgag
    16921 ataaacaccc atatggaaga aataagaaaa aataagaaaa gatgcgtatc caatacacag
    16981 aattttttgg aattgcgatt tctagatgtc tatctaaatt tcgattagtg gagaaacata
    17041 aaaaagggaa gtgcagcttt tgagcactcc ccttttttat aaagctttct tgtagcatag
    17101 tatcgaagga acctgccaat tctctactcg atcaccaata cgccttcttt tagtgcaaat
    17161 ggattttgat cgttgatgtg gtcatagaac atcacaccgt tgatatggtc gatttcatgt
    17221 tgaacgacga tcgattcata attcttcaaa cgaatcttgt gtttttcacc attcatatca
    17281 taataagaca cagtgatttt ggcatggcga acaacatatc ctggtacttc tcgatcaaca
    17341 gataaacagc cttcaccttc ccctaaacaa gcatcttgaa cagaatggct caaaatttta
    17401 ggattataca taacagtact tagagaaggt ccatctgcct ctgggtcagg acttggtacg
    17461 tgaacagcaa tgattctttt agaaatatct aattgaggag cagctaagcc aacaccgcct
    17521 cgtaagtgta gttcttcagc tttgattgga tcttggctgt tttttaagaa ctcaagcatc
    17581 tcttttccta aagagatatc ttcttcgctt aatggaagag aaacttcttt tgcaacttcg
    17641 cgtaaggtcg gatttccttc acgaatgata tcatccattg taatcatgga atatgtaccc
    17701 ctttcttaaa agactgtgtt tcaagtctat tttcgcatta tagtttacca caaaaatgga
    17761 aaagtagtag taagattgtt tgaaaactac cttttcttaa tgattaacta cttgattttc
    17821 aacccggttt caggtaaagt atagctgtgt gctctaggca catgacctgc cacttggtta
    17881 agcgaatcac caacgtttta ctcagtcgca ggggaataat cgaaagaggt gaaagaaatg
    17941 gcaatttcaa aagaacgcaa gaatgaaatc atcaacgaat acgctcgcca tgaaggggat
    18001 actggttctc cagaagtaca aatcgctgta ttaactgaag aaatcaacca cttgaatgaa
    18061 catgcacgcg tgcacaaaaa agaccatcat tcataccgcg gcctaatgaa aaaagttggt
    18121 caccgccgta acttgttagc ttacttacgt aaaactgacg ttcaacgtta ccgcgaattg
    18181 atcaaacgtt taggcttgcg tcgttaattt tagtcaaaaa gagtgggatt cacatgaatc
    18241 tcactttttt tactcgtata tcaattaagt aaaattccgt gatcaagcta gatggactcc
    18301 tactaaccaa atgaaagctg ctcaaaagac ggctttcggc agttttcatt tgtttagtag
    18361 cgtcttgctt tcacgagaag gaaaaggaga agttatgtca gaaaaacaag tattcaaaac
    18421 gacatggggc ggccgtccct tacaaatcga agtcggtcag ctagcaaaac aagcaaacgg
    18481 agccgtttta gtccgttatg gagatacagt agtcttaagt gcagctgtgg cctctaaaga
    18541 agcaaaagat acagactttt tcccattaac gatcaattat gaagaaaaaa tgtatgcagc
    18601 agggaaaatc cctggaggtt tcatcaaacg agaaggccgt ccaagtacgg aagcaacatt
    18661 gactgcccgt ttgatcgacc gtccaattcg cccgatgttt gcagaaggct tccgtaatga
    18721 agtccaagta acgaacatcg tgatgagtgt agaaacagac tgctcgccag ctatggcagc
    18781 aatgctaggt tcttctttag cactatcaat ctcagatatc ccatttgacg gcccaatcgc
    18841 aggcgtagaa gttggtcgtg taaatggcga gtacgtcttg aatccaacag tagaacaagc
    18901 agaacaaaca gatatcgaat tgactgttgc cggaacgaaa caagccatca atatggttga
    18961 aagcggcgcg aaagaagttt cagaagaaga catgcttggt gcattgctgt ttggttttga
    19021 cgctatcaaa gagttagttg ctttccaaga agaaatcgtt caagcagtag ggaaagaaaa
    19081 aatggaagtg actttactgc aagttgatga agtattgaaa aaagaaatct ttgatgcttc
    19141 ttacgctaca atgaaagcag cagtaatgac tgaagaaaaa cttgctcgcg aagataatat
    19201 cgagcaggtg aagatcgata tccgcgaagc ttatgctgag aaatttgcag gtcatgaaga
    19261 tgaagatcac ttgttgaaag aagttaaaca aatcacagaa gatcttgaaa aagatgtcgt
    19321 acgtgaattg atcaccatcg ataagatccg tcctgacggg cgtaaattgg atgaaatccg
    19381 tccattgtca tcagaggtca gcttattgcc acgtgtacat ggatcaggtt tgttcacacg
    19441 aggacaaaca caagctttat cagcttgtac actcgcacct ctaggagagc accaaatcat
    19501 tgatggttta ggggtggaag tcagcaaacg attcatccac cactacaatt tccctcaatt
    19561 ttctgttggt tctacaggac gtgcaggctc tccagggcgt cgggaaatcg gacatggcgc
    19621 attaggtgaa cgtgcgttag cacaagtaat cccttcagaa gaagaatttc cttatacgat
    19681 ccgtttagta gcagaagtat tggaatcaaa tggttcttct tcccaagcaa gtatctgtgc
    19741 tggcacatta gcattgatgg atgcaggtgt accaatcaaa gcgccagtcg ccggtattgc
    19801 gatgggactt gtttctgatg gtgaaaacta tacgatccta actgatatcc aagggctgga
    19861 agatcatctt ggcgatatgg actttaaagt tgccggtaca aaagatggta tcactgcttt
    19921 acagatggat atcaagattc aaggaatcac agaacaaatc ctaactgaag cattaactca
    19981 agcaaaacaa gcacgtatgg aaatcttaga agaattgact tcaacgattg ctgctccacg
    20041 tgaagaatta agccaatatg caccaaaaat cgaaatgatc cagatcgaac ctgctaaaat
    20101 caaagatgtt atcggtaaag gcggcgatac gatcaacgga atcatcgatg aaactggtgt
    20161 gaaaatcgat atcgatcaag atggaaaagt aagtattgct tcagctgatg cagaaatgat
    20221 caaaaaagct atcaagatca tcgaagattt gaccaaagaa gtcaaagttg gtgaagttta
    20281 tctagggaaa gttgtccgta tcgaaaaatt cggtgcattc gtcaacttga tcaaaggaaa
    20341 agatggactt gtccatattt cccaattagc aaacgatcgt gtcaataaag tcgaagatgt
    20401 tgttaaacta ggtgatgaga tccttgtgaa agtaactgaa atcgataaac aaggacgcgt
    20461 aaatctgtca agaaaagcaa tgttgaacga agatggttca acgaaaaatg aagaaaaata
    20521 atttaattca taggaataat gtacttgtga tcgtgaaggt gacaggggga caataattat
    20581 tcgaaaaaag gggactgtga caaaagccgt gtcacagtcc ttttttttcg aataaacggt
    20641 gttcaatcag ctgtccctcg
//
LOCUS       DACEUZ010000040        20222 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.040, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000040 DACEUZ010000000
VERSION     DACEUZ010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20222
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.040"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(50..1234)
                     /locus_tag="IXG44_001252"
     CDS             complement(50..1234)
                     /locus_tag="IXG44_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HAQ1657984.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(1259..2266)
                     /locus_tag="IXG44_001253"
     CDS             complement(1259..2266)
                     /locus_tag="IXG44_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ1657985.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            complement(2438..2797)
                     /locus_tag="IXG44_001254"
     CDS             complement(2438..2797)
                     /locus_tag="IXG44_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1657986.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            complement(2769..3140)
                     /locus_tag="IXG44_001255"
     CDS             complement(2769..3140)
                     /locus_tag="IXG44_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HAQ1657987.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            complement(3157..3459)
                     /locus_tag="IXG44_001256"
     CDS             complement(3157..3459)
                     /locus_tag="IXG44_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1657988.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            complement(3476..3946)
                     /locus_tag="IXG44_001257"
     CDS             complement(3476..3946)
                     /locus_tag="IXG44_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HAQ1657989.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            complement(3921..4199)
                     /locus_tag="IXG44_001258"
     CDS             complement(3921..4199)
                     /locus_tag="IXG44_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HAQ1657990.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            complement(4196..5245)
                     /locus_tag="IXG44_001259"
     CDS             complement(4196..5245)
                     /locus_tag="IXG44_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HAQ1657991.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            complement(5202..6131)
                     /gene="cpaF"
                     /locus_tag="IXG44_001260"
     CDS             complement(5202..6131)
                     /gene="cpaF"
                     /locus_tag="IXG44_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component"
                     /protein_id="HAQ1657992.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            6263..8017
                     /gene="ade"
                     /locus_tag="IXG44_001261"
     CDS             6263..8017
                     /gene="ade"
                     /locus_tag="IXG44_001261"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HAQ1657993.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            complement(8115..8777)
                     /gene="trmB"
                     /locus_tag="IXG44_001262"
     CDS             complement(8115..8777)
                     /gene="trmB"
                     /locus_tag="IXG44_001262"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HAQ1657994.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(8847..9626)
                     /locus_tag="IXG44_001263"
     CDS             complement(8847..9626)
                     /locus_tag="IXG44_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HAQ1657995.1"
                     /translation="MEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAAL
                     SREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLKT
                     MLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHGD
                     VNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDEM
                     LEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            complement(9833..11044)
                     /locus_tag="IXG44_001264"
     CDS             complement(9833..11044)
                     /locus_tag="IXG44_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1657996.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            complement(11037..11774)
                     /locus_tag="IXG44_001265"
     CDS             complement(11037..11774)
                     /locus_tag="IXG44_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1657997.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            11912..12346
                     /locus_tag="IXG44_001266"
     CDS             11912..12346
                     /locus_tag="IXG44_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HAQ1657998.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            12339..12683
                     /locus_tag="IXG44_001267"
     CDS             12339..12683
                     /locus_tag="IXG44_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAQ1657999.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            12863..13873
                     /locus_tag="IXG44_001268"
     CDS             12863..13873
                     /locus_tag="IXG44_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ1658000.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(13942..14886)
                     /locus_tag="IXG44_001269"
     CDS             complement(13942..14886)
                     /locus_tag="IXG44_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ1658001.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(14870..17590)
                     /locus_tag="IXG44_001270"
     CDS             complement(14870..17590)
                     /locus_tag="IXG44_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ1658002.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            complement(17587..18807)
                     /locus_tag="IXG44_001271"
     CDS             complement(17587..18807)
                     /locus_tag="IXG44_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HAQ1658003.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            complement(19088..19933)
                     /locus_tag="IXG44_001272"
     CDS             complement(19088..19933)
                     /locus_tag="IXG44_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ1658004.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
BASE COUNT         6174 a         4068 c         3324 g         6656 t
ORIGIN      
        1 aattccctct tgcatgcgtt tcatctttta tcctaacgaa tgctttggct tagccgcgca
       61 gacgctccac atcacgggca atcattagtt cctcatctgt tgggatcact aatactctta
      121 cttttgattc ttttgtagag atatctactt cttcaccacg caattcattc aacgctggat
      181 cgatttcaca accaaaccaa gacatacctt tgatgatttc actacgtaca tgtccgtcat
      241 tctcgccgat accagcagta aagacaattg catcaacacc attcatcgtt gtcacataac
      301 cgccgatgta ttttcggatg cggtccacga aaatgtcata agctaaacga atatgttctt
      361 tttcatagtt cttttcaaga tcacgcatat cactagacaa tcctgttagg cctagcagac
      421 cagatttctt gttcaaaaca ttgatcattt catcaatatc aatctctaat ttttccatta
      481 aatatgggag aacagctgga tcgatatcac ctgaacgtgt acccattgtc accccagcta
      541 atggtgtgaa tcccatagaa gtatccatgg atttacctcc atcgactgct gtgatcgata
      601 caccatttcc taagtggcaa gtgatgattt ttagttcttc gatcggtttg ttcaataatt
      661 ttgccgcacg ttccgacaca taacgatgac ttgtgccatg gaagccatat tttcttactt
      721 tgaaatcttc ataataagac accggtaagc tatataaata attttgcttt ggcattgttg
      781 agtggaaaga tgtatcgaat acagccacac tcaaaatgtc aggtaaaatc tttttaaacg
      841 ctttgatccc cattaagttt gcaggattat gcaaaggagc aaattctgct aattcagcaa
      901 tgcggtccat tactttctca tcgatcacta ctgaatcccc ataagtttct cctccgtgaa
      961 caacacggtg ccctactccg gtgatttcat cataagagct taaaatttgc agttcaatca
     1021 actgatctaa cagcattttt actgctactt cgtggttttc gatatccatg atttgttcga
     1081 atttttgatt atcgccgtac ttgatggtaa aaatagaatc ttgtaaaccg atacgttcga
     1141 cgatcccttt cgcaactacg gtttcttctg gcatttgata taattgccat ttcaaacttg
     1201 aactcccagc attgattgca attgttttag acatatgact ctccttctcg ttgaatgatt
     1261 acagagcaga tgatttccag tttttaaact cgttgaaaaa ttccgtcacc ttctctggtt
     1321 cttttaatga agcaagtttc acaagcaaga cttcttttac ttgttgtgct tgtggtcctt
     1381 ttttctgtaa gatcaaaata cttttttgcg attgtttatt acggaacaat tcatctggca
     1441 attgaatcat tccttgaaga taaccttctt ctttaaacca ttttttgatt tcttcacttt
     1501 gatccgtttc taaaaatcca ctcggcatca aaaacaaacc aaaaccatcc ggcttcacat
     1561 atttcattga ttgctctagc aataaatgat gtgcataact atggccttct tcggtacttg
     1621 ttaaaaactc ttttgctttt tcgtcattcg gataataacc gattggcaaa tcgctgattg
     1681 caaaatcgac aggatctatt aataattcct gcaacccgtc ttgatgaaag tattgaacat
     1741 ttgcttgtgt caaatcactt gtagaagctg cgacagccaa caatgtatca tcaatatcca
     1801 caccaattcc ttctgtttgg taccccgcat tcgataggtt taaaagaact gttaataaca
     1861 agttgcccat tcctgccgca atatccagaa tcttaaccgg tgctttttta tttgtgtata
     1921 gttgttcaat aagaaaaaca aataaaaaac cgatactatc aggagtgagt tgatggttcg
     1981 cctgcaaatg ttctgtctgg ctgcccttca ataaaagtaa ttgagacagt ctgcgccatt
     2041 cttctggctc gaaagaaagt ttcttcaact cttcataaag agctgtgatc cgttgcgttg
     2101 tctcttttgt cggcactcca tccaccacac gtacttgata atcatctatt aaattttctg
     2161 cattttcgac ataggcttct aaaaaggaag ttcccaatgc attctgtaaa agctgaatag
     2221 cttccaggtt ttgattaaac ccttgttcca ttttttctgg taacatggtg cacctcattt
     2281 ctagatatat tataacaatt ttttcacctg tttaattcta tcgaacaatt gacaaaattt
     2341 caatcgtttt cactgttttt tatttgtgaa acggcaaaca tttttttatt aaaaacagtg
     2401 ttataacaga aaagagggcg ttttcacgaa cattccatca ttctgtacta gtatctgtat
     2461 cattttttac ttcttctgtg aaccgaaaga taaacggtga gacttccacc tctaagaata
     2521 gggactgttg tacccactga tatgttacag atccagttgt gtagtaaaaa actccttccg
     2581 aactttcggc aagcgtagac tgttccgata aagccatctg cttcattatt tttgctatat
     2641 aataattttt gattcgtata ttaagatcca ttgacaaacg gtagctttct aatgctgcca
     2701 tcaataaaaa cgtacataaa agaaataaaa gcaagacaga aaataaaata gagcccttat
     2761 aacattttct actgatcgtt ttttgtccat atggcatatc catcttctcc attttcaaag
     2821 tgtacataaa aagaaattgt gtgttctttt tccaataatt gaagctcagt gatacttgtt
     2881 aacataggtt gatgtccgcc ggaagttctt cttttacgta tcattgcctg atatctttcg
     2941 atgatatact gtttatcctc gatcgtgtag tgcagttgct gatttttcac agagacattg
     3001 tgactgctct tgagttcttc ttctaattgt gctaaaaata ttagccactc tttttgatct
     3061 ttacgctgca aaaattcact tacttggatg gtttgctgaa tcaaaagatt gaatgtgagc
     3121 aatagactgc tcaatatcat cagtccaatt agacattcaa tcagcgtaaa tgctggaatt
     3181 acttgttttt tcaatactga tcgcttcttt ctccatattg acaaacactt tttcgattac
     3241 tccattatta tcgacaattt ggatatcgta tgggatcatt tcttcccttc gctctggtct
     3301 tttgtgatac atccgctgcg tcttgatatc ttcataaatc attcgaataa atagcagttc
     3361 ctccccactt ttcttttctg cctgaagcaa aaacagctgg ccaaagctca ctaagtagac
     3421 acagacagaa gcgatcataa gcgctgtaag ggactccaac aaaacaaatc ctgttttatt
     3481 caattctttt tgtatagcga ccactcccta tctggaattg atacgtaatc gtccgctctt
     3541 tttcttccca ataaaattga taagctttta gtgaactttc attcccggtt ctagcagcaa
     3601 aagtaataga agcatggcgt ttcaaggtaa tctcatcagg aatatctaat acttcccaat
     3661 ttattttatc tggattcgga acatgaaaaa tgatttgatt ctcttcacta ttccaataaa
     3721 agcctgtttg gatctgattc acaatagcga ttttttgcgt agaatagatt cgtttttcaa
     3781 attggccgaa aaattgatag actgccaatt cattcttcca tgcagaaaat gcgagaactg
     3841 gaaaagaaag gatcgttgta agtaataaaa tcagtaagag tgattcaaac aacgtatatc
     3901 ctgatgctaa aaccttacct tcaaggccgc tgatagattt ctgcatgttc tcgtttgata
     3961 tagccttcat tcaacaattc ctccacagta ggaactcttc cattattttg aagcgcatag
     4021 agctcttttt gtgtgtctac caactggatg atcgcttcac gactttcttg gtctacatga
     4081 ttcctataac gcgacaaatt agggacgaac aacaacacta atacactgat gatcagtaag
     4141 acgattagca tttcgatcaa tgtaaaaccg ctataataag atatttttct ctttttcatg
     4201 gcaaaacctc ctctatgctt ccgtaaacag gtaataaaac agcggcataa actaataaaa
     4261 tcaaagccgc tacgcccaaa aaaatgattg gctgaataaa atgcatccag tgatgaagac
     4321 gtttgaaaag ctgttttctt gtccattcgc tgtaaaaaat cagttcttta cctaaacttc
     4381 cctttgcctc tccctgtaaa acaattttac tgaattcttc tgatagaaaa cgataattct
     4441 ggaactgctc agccaatgac atacctgcct ccaatccttg aatcaactta gaggcaagat
     4501 gctgaatcag actttcttgt ttcgtttcct ttaagcagac tatgatttgc cttaattcga
     4561 aaccttcatg aaaaagcttt ccaatctcta gtgacaaata agccgattga tacaaggaat
     4621 atatcggtcc aatcaataat tgtctgctta tccattcact ctgatcaaca ggagacaact
     4681 tcatcagacg ataccttact agtaaagcca atagagtaac cgctaggatg aatagtagaa
     4741 cataccaatg aaagacttgt atcagctgaa taccccaatg ctcttgcatg accattcctg
     4801 aaccaataag ctgaggcaaa acgaacaacc gcatactaaa caagatacct agcaaaaagg
     4861 tgagcaatag catcggatat gcaagcagct ttttcaaatc attgcgaaat ccttcaacaa
     4921 gccgaaactg tttggcaatt tccaacaaag ttgtcggcag attcccatga cgttcagcta
     4981 actcgatctg taccagttgc tctgctccat atcctatttg cgaaagtacc tcactaaacg
     5041 cttgcccttc ctttagctga tgttggatat ttgccaaaat aacaggtgga aacgaacgga
     5101 tcgtgagtaa taaataaagg gattcttgca gactaaaacc agataaaagc agttctccca
     5161 tggtttgtgc aaactggttt tgctggcttc ttgttaatcc tttagaatag ttctgactgc
     5221 caaatatttt cctgagcttc ttcataactt tcctcccatg atttttttac tgattcttcc
     5281 tccataaatt gataactcca taaaacagaa ggatggtgat tggcatcttc caaaagttct
     5341 tgataaatga caccagctaa acattcagat agctcttctt ttttctttgt cagttcatgg
     5401 attctgacca atgtaccttc aagattgcgt gcatgtacgg ttgcaaaaac ccggtgccct
     5461 gttagtgctg ctcttaccgc agcatgtgca gttgattcat ctcttatctc gccaatgatt
     5521 agcaagtctg gacgatgccg taatgcgagt ctcaaaagtg tatcataagt ctgtgctatt
     5581 ttcggattga tctgtagctg cagaaaaaac ggttcttcta tctctacagg atcttcgata
     5641 gtaatcactt gcaatttttc ttccaaagcc aaccgataca ttagagaagt cttgcctgat
     5701 ccgactggtc cgctgaaaag gtataatcca cgtctgacgg ttttctgtaa aatagttgca
     5761 aaatctcgag aactaaagca acgatggggt gtttcaaaag taccgtataa tagccgaatc
     5821 actaaactct ctcgttgaag gtaatctcct acagaagaca accgcagacg aacttcctgt
     5881 ttttctaaag aataagtaaa tgcacctaac tgagcttttc ttttttctcc gatatccatc
     5941 tgtcccaaat acttaaatct tgagatcagt tgctgtccat ctgccgaaga gactttcttc
     6001 cagaatgttc gtttttttcc atagcgaaaa tagatgtaaa atacttctga agctggcaat
     6061 atatatacgt cttgggcatg tttggcaaaa ctttcactta tcatttgatc cgataatcgt
     6121 tttatttcca ttattctccc ctccacaaaa aacatacgtc tttttgatct ttttgtttca
     6181 aataaaatga atttacaaaa aaatattggc aaaaaccgaa tttatgttat acttataaaa
     6241 atttatcttg ggaggcactc cattggaaac aacattaagg aaatatatcg aagtagcaag
     6301 taaaaaaaag cctgctgacc tagtgatcaa gcatgctaag atcgtcaacg tctttacgaa
     6361 agaaatcatg gaaaaagatg tagcaatctg cgaaggaatg atcgtaggaa tcggtgaata
     6421 cgaaggaaaa agcgtacata atgctaatgg gcaatatcta gtgccaggat tcatcgatgg
     6481 ccatgtgcat atcgaaagct cacttttatc accaagcgaa ttttcaaaag tatccttgct
     6541 tcatggagtt actactgtag tgacagatcc ccatgaaata ggaaacattg ctgggagtac
     6601 aggtcttgag tttatgatag aagatgcaag acaaacactc atgaatatct tttttatgct
     6661 accttcttgt gtacctgtaa ccccttttga gaccaatggt gcaaatttaa atgcaaaaaa
     6721 attagcccct tttttacaaa aacccgaagt tttaggctta gctgaagtca tgaactatca
     6781 agcagtagca aataatgaaa cagacatcct agaaaagatc caattgatgc accagcataa
     6841 gaaaaaaatt gacgggcatg cagctggtat cggcatggaa gagttaaatg tttatcctgc
     6901 agcaggtatt cggacggatc atgaagctac aactgcaaaa gaagctaaag aacgtttgga
     6961 tcttggtatg tatttaatgg ttcgagaagg gaccgttgca aaagatctgg catcgctact
     7021 accggcgatc acacctgaaa attcaagaag atgcttcttt gtcaccgatg ataaactgat
     7081 caatgattta gtaaaagaag gatcaatcga tcatatcgtc cgtcaagcta ttgcacttgg
     7141 aatcgatcca ttacaagcca ttcagatggc aacattgaat gcagcagaat gttttgattt
     7201 gaaaacacta ggtgcgattg cccctgggta tcaagctgat ttcttcttaa cagacgatct
     7261 taccaagctg ccaattcatg aagttttcta taagggaaca ttagttgttc aatctgggca
     7321 actccaagac catttatttg ctgatcattc aaattcatat acgtgttgtc tgccaaaact
     7381 aaacgctcaa ccattaaatc cacattcgtt agagctgctt ctttcttctt cacaagccca
     7441 tgtgatcgaa attatcccga atagtctagt gactaaagat ttggtagaaa aagtggaccg
     7501 acaaggcgaa cgattcattc cttcaatcga aaaagaccaa ttaaagattg cggtgatcga
     7561 gcgtcaccat caaacgggaa acattggatt aggcatagtg aaaggatttc agctgaaaca
     7621 aggagcgata gcgacaacga ttgcccatga ctcgcataat ctagttgtcg ttgggacaaa
     7681 cgacgaggat atgctgtatg ctgctaatct tttgattcaa aaaggtggcg ggatgaccgt
     7741 tgtcaacaaa cagcaggaaa tagcttgtgt ttctcttcct ataggcggaa tcatgacaca
     7801 ggaacctttt tcgatcgtct atgaacagtt gaatgaatta ataactaaag cacatcaact
     7861 aggagcagct aaaacctttg atcctttttt gacactttct ttcttaacat tatctgtcat
     7921 acccgaattg aagatcaccg acaaaggatt attttcattt tctcgcttcc aattgattcc
     7981 accttgtgga aaaaccgatg aaataaatac attttaaatc aaataaatcc cagaaaatac
     8041 attacatctg taatgttcat tttctgggat ttatttatgg atattaatgt ttttatgaaa
     8101 gcagaaagtt agtttcacag acttttcttt ttggtcttat cttgaaattg agcttctact
     8161 cgataaatac gttgaccttt tgaagaaaat ttttcttcat actccgtcat aatattgcct
     8221 tcaaattgac tttgatgtaa atctaaccat acctgtttga ggatcatgcc atattgagaa
     8281 aaacttgcca gagaatactc aaacaatcct tgattatctg ttttgaaatg gatttctcca
     8341 tttggacgaa gaatctgttc atctacagca agaaaagtct tatatgtcaa tcgccgtttt
     8401 tcatgacgtt tttttggcca tggatcggaa aaattcagat agatttgatc tacttcgctg
     8461 tcagcgaaat actctgtcaa cgctgatcca tccacatgta gaagtttcag gttaggaagc
     8521 ggttgttcga tcaacttgtc tagtgcaatc gaaacaacac tgacctgcat ttctactcca
     8581 atataattga tttcaggatg ggcttttgcc attccagtga tgaactggcc tttccccata
     8641 ccaatctcaa tgtggattgg atgatcgttt tcaaacaatt cattccattt tcccttccac
     8701 aatgttggat cactaattac aaaattcggg tgagccgcta acatttccgc tgctcccgga
     8761 cgatttcgga cacgcattat ttatctccat tcctttcctc taattaaaaa ggtctggcca
     8821 aatctccgca gagtttgggc caaacctcag ccactgaaaa ttcgttttaa ttgtaaaatt
     8881 tcttcgttca tccgtcgatg atcttcacgt ttgcaatatc tggtgatttc ttggagcaaa
     8941 ttcatggccg cataccaatg aaatttttca agcatttcgt cagtcgggcg tatgccataa
     9001 ctgatcagcc actgattcca gctagacagt ggcacataat ttcctaaaat cgttgcaata
     9061 tccaccgcag ggtctgcaaa catgattgaa tcccaatcga ctagatataa ataattttta
     9121 catactaacc aattacgatg gtttacatct ccgtgtacta ctgataagtt ttccttatct
     9181 attgacggga tctgctcacg aagaaaacga tggacaattt gcagaaattg atttttctgg
     9241 agttcttccg gtaattcttt ttcatatttt tgcagcatct tctccggcgt catgacacga
     9301 ccaccgatac gggtcagcat ggttttaaga gaatttgaat gatgtaaatg gtacaaaaca
     9361 tcaatcacgt cgtttctttg accaatctca ttaggagtca gcaagcgacc ttccagccac
     9421 tcttgtgctg tcaaagtatc gccattcccc gttcgttttg tccaaacaag ttttggtgta
     9481 attccttccc ttgaaagagc agccaacatc ggagtcgtgt ttcgtttaat aaagactttg
     9541 tcctcatcac gcatgcctat gtatgttttt cctgtgtcgc ctttgatggg acgtaaccgc
     9601 cactcttggt ctaactgaaa ctccatcatg cattctcccg tcctcttact ctttgttttg
     9661 cagtacttat tattctagtc gcctgaacac tacacgtcaa gcacatatac gttctttttc
     9721 atttaattgc cattcaaaaa accagctttt ctcaaaaaaa atagaacgaa cagcattctt
     9781 actgtcaacg ctctattttt cattctcatt tttaccctac acagctttgt tttcagtctg
     9841 acatcttctt caaacgagtt ggtacatata gtttaacaaa gagcatagta acaatcaaat
     9901 aagccgctag cggaacccat cgctgctgcc catctaatac gactgcagca atcaaaccaa
     9961 aaataaccga cgcgataatt aatacccatg tgagtagata ctgaatcgcc tgcttcttct
    10021 gattcttatg gattggataa agctgaacga gaaccatata gcggaactgg ttcgctaaag
    10081 gtaacagttg gaaaccgatc aaatataaaa ataagaaacc gataatcaga ctaaaataaa
    10141 agtcgactac tccggcaatc aataacccac ctataattac taatcggaaa taaagaccac
    10201 taaaatcaga acctcttgcc aatcttcttg caaacaagta aagataagtg ttttttgatt
    10261 ctcttttaat atgagtcaat ataggatcca aatagcgtct tcttttgatc tttgccgtaa
    10321 tttcaggaac atctgtgaat aaattgataa attgatagat ctgatgcaac ctatgttctt
    10381 ctacatgcac cattttctcc caatccaatc ctttttcttg ttgcttccac aaaaaccagt
    10441 aatatagact tgcctgtact aatgcacctg ctattcctgc ccatattagc caataaaatg
    10501 atagaaacaa aaagatcaga gaagaaaaaa accagcctaa tttccaataa cgaataactt
    10561 gttgatctag tcgaaacaat tctgctctct tgatttgcag atgactgaat ttcaaactcc
    10621 ataatatgat cagatagacg aaaaacatcg aaaaggattg ctccgtacta accaccacta
    10681 atggcatggc caatccgcat gccaatatca aaaagatcaa cggaaaaatg caagaatagc
    10741 gaaaagctcg ttccaaatag gtttgcattt ctttttcttt aggaagtaaa aaaacaatat
    10801 ctgctgcctc tgcaaaagta gccatcttac ccacgaataa acagagcatc caaaatacag
    10861 agattatgag ccctccaatt gcaaacggtg gctgcagggt tttcaaccaa ttagaataat
    10921 ataatcccac accgccaatc aagaaaagac aaactaaaac aaaatgatcg ttcaatacat
    10981 aacgcatata tttcatcatt ttcttttgat gtcttgctaa acgtttttga aaaaaactac
    11041 tcatgtgcct gctcctttgt taaagcaata tagatttcgt ccaaagaaga atccggcagc
    11101 tgaaattctt ttctcaacat ctcaagagaa ccgtctgctc gtatctctcc gttatgtaag
    11161 accacaaaac gatcacaata tttttctgca gtagctaaaa tatgcgtaga catcaaaata
    11221 ccggctcctt cttttttcat ttctaccatc aattcaagca atgcattgat agctagtgga
    11281 tccaagccta aaaatggttc gtcgatgata tataaagaag gctcgatcaa aaaggcgcat
    11341 aagatcatca ctttctgttt cattcccttt gaaaaattag ctgggaacca gtcaagacga
    11401 ttagataacc gaaaagtctc caacaacttt tctgctcttt tcattgctat atccataggt
    11461 atatcgtagg ccatcgcagt gacttcgata tgttctctca atgtcaattc ttcatataaa
    11521 gaaggagtct ctggaatata cccaatttgt ttgcgatatt cttccgtatt tttctttaag
    11581 gatagatgat tgatttctat cgttcctttt gaagcttcca gcagaccgat aatgtgcttg
    11641 atggtcgtac ttttccctgc tccgttcagt ccaatcaacc ctactatctc tccaggctgt
    11701 acatcgaaac tgatatcttt taacacaggg agatgtccgt acccccctgt gacatgatcg
    11761 ataattaaac tcattctttt tcctccgcca tttattttta tttattatac cacgaagaaa
    11821 atgacggtat tttgtttttt tccttacaat agctttaact tatggtaaag ttaagctgaa
    11881 gtatttattc tttatagaaa ggatgtttta tatggaaaac tgtatttttt gcaaaatcat
    11941 caatcaagaa atcccaagct ataaaatata tgaagacgac aaggtctatg cttttttaga
    12001 catctctcaa gcaacgaaag gacacacatt agttgtgcct aaacgtcatg tagcagatat
    12061 ttttgaatat gatcccgaac ttgcaggaga ggttttttcg cgtgtgccta agattgctca
    12121 agcattggaa aaagcttttc cagaaatgaa aggtcttaat atcttgaata acaaccgtga
    12181 actagcctat caatctgttt ttcattcaca tatccattta gtccctcgct acagcaaaga
    12241 ggatgatttt tctattcact ttgtcaatca tcaagatagt tatggatcgg aagaactaaa
    12301 agctattcaa gaaacaatcg taaagcaggt gtcctgcgat gattaaaaac tttttcaaag
    12361 gactgttatt tggagctgct gctggcactg tcggaggtct gttattggcg cccaggagcg
    12421 ggaatgaaac aagaaagaaa ctgatcgaag aattggaaga agcaacaact ctgacaaatg
    12481 atttaaataa cagcctgaat cattttaaaa gcgcattgat cgaaacaaaa gaaacagctg
    12541 aatctgtgat tcctgctttc caagaagcaa tcaaaaaaga tatcgaagat tttaaattcc
    12601 aagccgaacc acgtatcaca caaatcaatg aacaagtaga aaaactaaaa gctgatctgc
    12661 cagagtatcc tgaacaaata taataatgat tattttctca acagatttaa ctatataagt
    12721 gagaaaagtt tgcgtatttc ctaggaatgt gatgaaattt tgaattttgt ttgaaactac
    12781 cacgacaacg tttttaaagg aaatgggata tggtatagtt aatatataaa aataataact
    12841 aattacagga gtgcaaattt taatgaaaaa aaaatcaatt attttggcag ctactagtgc
    12901 gttggctgtt ttaactctag cagcttgttc aggcgatact aataaagata tcgcaacgat
    12961 gaaaggcgga acgatcactg tatctgattt ctatgatgaa gcaaaacttg aatcatcgaa
    13021 ccaatcctta gtacaacgga tgatcattta taaagtattc aataacaaat atggcgataa
    13081 agtaaccgat aagcaagtgg atgcagaata cgacaaacaa gcgaaatcat taggtgatac
    13141 ttttgaatca caattagaag ctgctggata tacgaaagat acttataaag aatatattcg
    13201 taacaattta gcttttgaag ctggcttgaa agctcacgta gatattacag atgacgattt
    13261 gaaaactgct tggaaatctt tccatccaga agtagaagca caaatcatca aattatcaag
    13321 tgaagatgaa gcaaaagatg tgaaaaaatc agcggatgat ggagatgatt tctctaaatt
    13381 agccaaagac aaatcaactg atacagagac aaaagaagat ggcggtaaag ttaaattcga
    13441 ctcaacgaca acaactattc ctgcagaagt aaaagaagca gctttcaaat tgaaagacgg
    13501 tgagatctcc gatgttatta ctacaacaaa tcctacttct tatgcgacag aatactacgt
    13561 tgtcaaaatg gtgaaaaacc aaaataaagg caatgacatg gataaataca aagatcaact
    13621 aaaagatatc gctactgaaa ctaaactaag tgacaatgca ttcacaacta aagtaatcgg
    13681 tgaagaatta aaagatgcaa atgtaaaaat caaagatgat gcctttgaaa atgtgttgag
    13741 tgcattcaca acaacttcat caagtacgaa agactcaagt gaaactacag cttcaacaaa
    13801 gagttctgac acaaaatcaa ctgattcaac aaaagaatct tcaactgaag aaacaactga
    13861 ttcatcaaaa taaaagaaaa caaaaaaagc gaagacttat gtctcgcttt ttttgttttg
    13921 aatgaaaaaa ataaattttt gctataaatc ttttggtaca tagaaacttc ggttgtccat
    13981 accgaaaatc cgatcggtgt attttcctgg ttctgtttga cgaatcgaag tcaacatcat
    14041 ctgcatcgaa gcatcgatat tgtcgatttg atgtaatatt tctgcttcca ttattcttgg
    14101 acgtacaggt gagccatatt ctaataagcc atggtgtgct aaaaccatat gacgtaatac
    14161 gagaacatct tcgtcagctt cgctgatttt caacgcaaga caggctttag ttatttcttc
    14221 gtcaatcaat accagatgac cgatcaaatt tcctgcaagc gtatattctg tcgacatagg
    14281 accggaaagt tcaatcacct ttcctaaatc atgcaaaatg ataccagcat acaacaatgc
    14341 tcgattcagc tcaggatatt catcggcgat tgatttccct aagtgaagca ttgtcaaagt
    14401 atgataagct aatccactgg caaatgcatg atggtttctt tttgctgccg gaaactcaaa
    14461 aaattgtttt tgatatttat taagtagata gcgaacaata cgattccaat gcgcattggt
    14521 gatctcaaac aacaattgat tgatttcttc ttccatttct tctttcttca aaggggctct
    14581 ttccatgtat aaactaggct ctcctggctc atctaccctt gttaagcgca tatgaagaat
    14641 ctttacttga gggtttcctt gataaacctc tcttttaccg tttagaaaaa cgactgtgcc
    14701 ggctgtaaat ttattgattt cctcctccga agcatcccag tatttaccat cgatagtacc
    14761 tgaagtatct tggaaagtaa aagcaataaa tttttttccg tttttagcga ttcttacatc
    14821 tgcacttttg atcaggacaa attgttcaaa ctgctcatcg attactaaat cacgaatttt
    14881 tttcattatt tgctccttct aattgtttta cgatttggtt catctcatgg taatagctta
    14941 ccatttcttt atcagaagaa aaacaaatta tttgatactt ttctccaaat ttagctaaaa
    15001 gttcagctaa ttgatacttt cgctgatgat cataatgaag ccagccatca tcaatgatga
    15061 tcgggcatat cgaacggtta gcttctaatg agaggtaagc aaatctcatc gccataatca
    15121 gctgatcttt cgtacctgta gataactcat agatgggaaa ttctgtatat ccgtctgtta
    15181 aagtcagtgt atcatttttc agtaatatct tctgataatt tccttttgtt aaaataccta
    15241 aataatcacc tgctgctctt aacaactggg gtaattgtct ttcactcatc tctgttgaaa
    15301 gatcacctaa gaatgtcccg gcgatttcaa gtgctcccca ttcctccagt aattcctgta
    15361 attttgcttt ttgccggcta gcttgttgat aaagttcgtc caatgtgccg tctctttgca
    15421 actcttctac ttgaagttgg agtcgttgcc gattttcact tgcttctttt tcttcttgct
    15481 ggagttggga ttgcttttct ttcagttggc gcagtcttac agataatttc tcttttgtca
    15541 gctcttcagg aaatagtgga gtaagcattt ccgataattc attgtatctt ttcatctgct
    15601 gttcggcttg tttggctttt tgtaaaaaga caggtacttc cgaaggataa gataagcctg
    15661 cttgagtcaa ttgttcgctg tattcttgta tccatacttt ttgtttcttt ttcaattgat
    15721 tgatttcttg cttcaataaa gtattttgct gatagcttct ggcaaatttc atctgttcca
    15781 tctcttgtat aaaacgggaa agcaatacca ttttttctga aattgtctta tcttgtatcg
    15841 gtaaccattc tttaacaaac tcaaattttt ggtcgatttg tcgtagtttt tcttgtattt
    15901 cctgttctct tttatgtttc cgctcaaata cgccatataa tttctgataa tctatgactt
    15961 cctgtccata ttctttcatg agtttgagcg tatccatctt tcctaagtgc cgatgatccg
    16021 caaaacgata tacttcttgc tgaagaatct gtttttcttc tccagttctc ttttgttttt
    16081 cttctgcttc agctaattga gctaaggcta gatcaagttg tcctagtttt tcttgccaca
    16141 ttccttttat ctcagagaaa tcatttttag ttttaatttt tggttgcctt ttgtagaaga
    16201 aatagccaat catgcctgca ccaattagta aaaggatcac tttcaagggc attggtacaa
    16261 atatacctgc aatgattatt gctccgccag ccatcagcat atacggttta gatgatgaga
    16321 ctggtgctat ctctgactga ttttgtaata atgctggata ggtctcttcc aagtcattca
    16381 cttctgcttc aatcatctct tgctgttctt tagctatttg gagacgcaca gaaatctggc
    16441 tttctagatc agcaatcctt ctttcttcag ccagaagatg cttccattct tcttcatata
    16501 aaaattcttc cggtaacgga tttttctgcc aatgccacct ttgttccaaa atcttcaatt
    16561 ctgaatttgt ttctctagtc tcaatttgta atctttgcca ttcttcgttt aattgttccg
    16621 ctgtaaattg aagttgctgg agatctttta gttcctgctc ctgctctaaa taaaaataat
    16681 atctggctga ctgctgatcc atcccgctat gcttttccag tgttgcttct aattcttcca
    16741 aacgctcatt tgtttgctga taccgtttgg agaaagactc cagatctaaa agaagttgct
    16801 catcaggaag acctgtagtg ctgttttttt tgagagattg ccattcttca tataaagaaa
    16861 aattaagttc ttgctgtctg gctaatgaaa attgtttgtt cgcctctttt aattgtgcca
    16921 ataactgctg ctgttgcgac tcgtattcct gttcttgttg gatcaacttg ccaaaataag
    16981 cttcctgttc ttgtttttga tgaatttttt cttgcagctc tttccattca tttaattttt
    17041 gattgattag taatttttgg ccttttttct tgaataattg ttgtgctttc tgataatatt
    17101 cttgccgctt ttggaatact tgagaactgc ccgtgatgcc taaagaaatc agcgcatcat
    17161 gtagctcttt ttctctcaaa tgatctagtt ccatcaactg ctcttgctgg aaagtaaata
    17221 cctgctggaa taactctttc gtcaaaggtg cgatcagttt ttctagtaac tcatcacttc
    17281 ccacttgatc ccccagccac accttcgatt ttccgcgatt ttgctgcttg tagcgctcga
    17341 cagcaaattc accatataca ggatgtttaa gccatatttt gccgccatag gctgagccgt
    17401 cttttggtgt ataatccttt tttttacggc cttttgaagg gaaaccaaat aatattgctt
    17461 gaataaattg ataaatcgtt gattttcctg cttcgtttcc cccatagatc agctgattgc
    17521 ctgaataaaa gtcaaaactt ttttgacgcc attttccaaa agcagcaatc tcgatcctta
    17581 aaatcttcat tcctgatcct cctcaaataa aaaatcttga ttaagcagct gcttggcttt
    17641 ttcaagtgtt tcttcttgat attccggcag ttcgctcaat attctagccg cttcttgatg
    17701 actatatact tcatttaaaa tttgttggaa atcttgaggt gtctgatagt attgaaataa
    17761 ctgttccatc aatttagcag aagcagcaag tggggtcttc gtataaaaag tattctctat
    17821 gagagaaata cgccaaataa agaaatcatt tatcctatct gagaattcct ctaacaaata
    17881 gtctaatagc tctcctgatt gtatccgttc caaaacatct gtattgagat ggtcataatc
    17941 ttttaatttt atctcaatta aactaaatcc atcctctact tttgaaagct gctgacgaat
    18001 gtgaacaatg acctcttttg tcgttcttgc tttcctcaat gatatttcct ttgtcttcca
    18061 atagacctct gctactttaa tcggatgtat ttggcaactt cctttactta attcaactag
    18121 tagaatagat gtagaagctt gttccttttt agtatgacct tgaggcgcac ctggataaac
    18181 aattgtttgc ttttcattta agaccgttgg cacatggata tgccctaaag cccaataatc
    18241 gtatcccttt tcttgcatct ttgagggttg gaaaggagca tagtttcctt tttgagagac
    18301 gccaggttcc ccgtggtaca acccaatatg gtaatctgtg agttctctag aaggaaattc
    18361 catcactttg tctttttgaa tccacggttg acgataacta aatccactga gagagacagt
    18421 ttctccactt tttatcgtta ttttttttgt ctctaccgtt tcggatgtaa ataaatggac
    18481 atttttagga aaggagaacc aataccgctc tttttgatag aaatcatgat ttccgaatat
    18541 tataaataca ggtatatggt tcttttctaa ccgtttcatc tgttctgaaa aatgtttctg
    18601 tatttttaat gaaggacgat tttgatgaaa attatctcct gcaagtaaaa caaaatcaac
    18661 gttatttatt attgcctgtt caatgatatt tgataaaacg gtcagattcg tcttcaatag
    18721 tttttcctgt acagttttat caagagttgt cagtccttca aatgaccgat ccatatgtaa
    18781 atctgccgca tggataaatt tcaccattaa aaatttcctc ctttttttct tttatcatac
    18841 cattttcaat gttttttttc atcaataacc gaaaatttgt tcgtattatg caaagaaatt
    18901 acgataaatg gccgacttca aaaaataagt ttattcgaaa aaaagattgt gacatgattt
    18961 ttgtcacaat cctaaatatc tatgttatat tcgcttcgtt tgacactgaa gaagtactat
    19021 ttgtactgga atttatgctc catttttccg tatttagaat tctatgttct atactacctt
    19081 aattagttta tatggtaaga attctttcaa atgcttgaga tactccgtct ttatcatttg
    19141 aaacagtgac ataatcagct tttttcatga tttcttgatg actatttccc attgcaaaac
    19201 taatatctgc actttcaaac atagataagt catttaaact atctccgatc gcgattctca
    19261 aaggtttctt taaattgtag attgattcca aacgatcaaa caccacacct ttagatacat
    19321 tttgaggtac gatttcaata tttacaggtg ccgattcagt aacagacaca tttagcatct
    19381 ttttcaaatg gttaaatata ttagctttca attgacgatt aggaataaaa ataaaaaatt
    19441 tgtaaatgct gatagcatcc ttttctataa tttcctcaat attattataa gggattgaac
    19501 aaatagattt ttcatactca agctcatagt ttagctcttt gatagaaaat tcactcccta
    19561 cctcgtgaaa aatttttttc attttatttt tataattctg agattcataa cttccgagat
    19621 ttgtatatat tttggttgga aatttcgata ggacagaatc aatttttttt acatcatttt
    19681 tatcgaagga agactcaaat atcttttttt ctccatcaaa tccaacagaa ccatttaacc
    19741 ctactgctgg tatccttaaa ccagattttt tgagaatatt attaatgtct tctacttctc
    19801 ttccagaaca aatgaatgga aaacagtctt ccggtaaatg tttcattgtt cttgcatttt
    19861 tttgactaat gctctggtca gaattcaaaa gagtaccgtc taaatctata ccaattagtt
    19921 tcatttcatc catgtctcct atacgttgat tataaattca ctgcttattg taagtctaaa
    19981 tttctcacaa aacatatact aattatacct aatttaagta agtacaaaca tcattttaag
    20041 aataggaaag gacatttata tactattctc tgtatttttg atttataaat aaatatgaat
    20101 tgaatccaaa tcagctatat tactatttta taataaccaa ctataaaaaa cgaagttagg
    20161 tgaaaaacta acttcgatat tcataaaaga ctctattagt tcaacttagc aaaagattta
    20221 aa
//
LOCUS       DACEUZ010000041        19448 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.041, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000041 DACEUZ010000000
VERSION     DACEUZ010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19448
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.041"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            129..3656
                     /locus_tag="IXG44_001273"
     CDS             129..3656
                     /locus_tag="IXG44_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAQ1658005.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3653..7375
                     /gene="addA"
                     /locus_tag="IXG44_001274"
     CDS             3653..7375
                     /gene="addA"
                     /locus_tag="IXG44_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HAQ1658006.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7444..7785
                     /locus_tag="IXG44_001275"
     CDS             7444..7785
                     /locus_tag="IXG44_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1658007.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8094..9137
                     /gene="pheS"
                     /locus_tag="IXG44_001276"
     CDS             8094..9137
                     /gene="pheS"
                     /locus_tag="IXG44_001276"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HAQ1658008.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9142..11562
                     /locus_tag="IXG44_001277"
     CDS             9142..11562
                     /locus_tag="IXG44_001277"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HAQ1658009.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11732..12742)
                     /gene="trpS"
                     /locus_tag="IXG44_001278"
     CDS             complement(11732..12742)
                     /gene="trpS"
                     /locus_tag="IXG44_001278"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HAQ1658010.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            12996..13733
                     /locus_tag="IXG44_001279"
     CDS             12996..13733
                     /locus_tag="IXG44_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ1658011.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13747..14562
                     /locus_tag="IXG44_001280"
     CDS             13747..14562
                     /locus_tag="IXG44_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAQ1658012.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14575..15222
                     /locus_tag="IXG44_001281"
     CDS             14575..15222
                     /locus_tag="IXG44_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ1658013.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15238..15894
                     /locus_tag="IXG44_001282"
     CDS             15238..15894
                     /locus_tag="IXG44_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ1658014.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16056..16877
                     /gene="racE"
                     /locus_tag="IXG44_001283"
     CDS             16056..16877
                     /gene="racE"
                     /locus_tag="IXG44_001283"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HAQ1658015.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16880..18235
                     /gene="rph"
                     /locus_tag="IXG44_001284"
     CDS             16880..18235
                     /gene="rph"
                     /locus_tag="IXG44_001284"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HAQ1658016.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18236..18754
                     /locus_tag="IXG44_001285"
     CDS             18236..18754
                     /locus_tag="IXG44_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAQ1658017.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18861..19361
                     /locus_tag="IXG44_001286"
     CDS             18861..19361
                     /locus_tag="IXG44_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ1658018.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
BASE COUNT         6538 a         3340 c         4177 g         5393 t
ORIGIN      
        1 tgcgtatact taaaaaagta gaaatatcag aaccttaaaa ggatataatt tgaagaatat
       61 agaacaaatg ttcagtattt tttgatataa taaaggagca ccgtatcatg tgttgaagga
      121 ggaaaagcat gtctttacaa tttattcttg gaaacgggaa aaaagatcac cgtcaagtct
      181 tgatcgacga agcatccgct tggctcgacc aggatgcaag taaccaagta tttttccttg
      241 ttcccaacta cagcaaattc gaacaggaac aagaaatttt agcggagatg cgaagaagaa
      301 gcggaaaaaa aactttcagc acaacaaacg ttcaagtatt tagtttttat cgtctggctt
      361 ggtatttgct gcaacagaca actcttctta caggaaatga attatcagaa tcaggatctg
      421 caatgatctt gcgacagatt ttagaaaaaa atgaggaaga attaacaatc tttcgcggag
      481 aaatcaataa aagagggttt attcgtcaat tacaagaact ctacagtgaa ttgcgtacag
      541 gaaatattca accagaagac ctgatgcttt tatttggtga ttcacctaat gcagaagacc
      601 agcaacttaa aatgaaagac ttgaagctga ttttttcagc ttatgacagc gaattgactc
      661 aacgtgcact aacaaatgaa gatgcactat ctatactagc aaattatatg cagacacaag
      721 atttcaacaa cgtcaaattt attgtcagtg gtttttctcg aatcaatggt caagagtatc
      781 aattactgca ggcaatgatg gaaaaggggc aattggttgt cgatttattg ctggatcgtc
      841 cttatacagc ggatcttcca gaagtactta cgctctttca tgagactgga aagctttatt
      901 tccgtctttt tcaaagtgca agagaacgga aagtccctat attagtggac cgatttgcac
      961 cagagcagac tcgtccgaca gagcttcaac tgctgcaaac aatgtgggaa gagacatcta
     1021 gccaaaagaa agcggcagta cacaaaatag ggggacaaga gcatctccat ttgtgggaag
     1081 ctgaaaatcc aactgaagaa gtccgacaat tagctgttga aatcagacga ctagtcagtg
     1141 aagaaaacta ccgttatcgt gatatccaag tgctgacaaa aaatatgtct ttatatggaa
     1201 atatccttcg gccgatcttt caagaaatgg caattccttt ttatatcgat gaagaacaaa
     1261 tgatggaaaa tcatccgctg atcgaatgga tcaacagtct gttcgctctt gaccgctatt
     1321 cttatcggct aagtgatatt atgcgttttt tcaaaactga attatttctg tcgacggaag
     1381 cagaaaccga acttgatgaa tggaaacaag agcgagatga attcaggaaa aaactggaca
     1441 tcactgaaaa tgtagcactt gcttacaatt atcaaggaac ttattggact cgtgaaaaag
     1501 actggcagtt tgtggcgtat gattttgaag cagaacagat tgaagacgta cagtatttgg
     1561 aagaacagtc aaatacgatt agacgattat ttcagaaagc tgtaccagta tttttgaaaa
     1621 aaataaagaa aagcaaaaca ggacaagagg ccgcaaatgt tttttatcaa tttttgatcc
     1681 aaagtggcgt acagcaacaa ttattgtttt ggcgtaatca ggaagtagac agaggaaact
     1741 tagaagcggc aagaaaccat gagcagacat ggggagcgct gattgatctg ttggatgaat
     1801 atgtagtaat ttatggtgaa tcggcttttg actggtcact gttccaagaa atcattacaa
     1861 gtggtctaga aaatctaagt tatggtaaaa tccctaccgc aatcgaccaa gtgcgaatca
     1921 atcgtttgga attagttcga ccaaatcaag ctgccgtcac ttttgcatta gggttgaatg
     1981 atcaagtgtt tccagaccga aaagagacaa aaggattgtt gtccagtgaa gaacgagaag
     2041 tattaaatca ggccttgcca gatggacagt ttttattcga cccatcaaag gaaagtatct
     2101 cttttgagcc tttccaagcc tatcttgttt ttctttccgg aacagatcga ttatatctta
     2161 gttatgcaca aagctatgac acagatggtt cgctgaagat gtctccttat ttgcgtagaa
     2221 tcacagaata tttatctgtt cccgtcgaga gaaaagagca tttgacgatc gaaagcgacc
     2281 ctaattgtta tgtagggact tacagaagcg gaatcaatac agtcaatagg atttatcgat
     2341 tagctttaga tgaaaaaaaa cctgtaccaa agcactggca aaagctcaaa gaattagtac
     2401 tgaattcgtc atggcgaaat tttgctttac agatttttga aagtcaggaa catttaaatg
     2461 ttcctgtttc gatgccagaa aaaatggcgg aagagcttta tggaaaagag atttatactt
     2521 caatttcacg tatggagaat ttttataatt gtgaatacaa atacttcatg caatttggtt
     2581 tgaaattgaa agaacgaact gtttatggtc taactccagc agcaactggt gatttttatc
     2641 atgaatcatt ggatcgtttc ttcaagctac tgttttcaaa ccaattatcc ttagtgaata
     2701 tgtccgatgc gcagcgtaga gactttactg aaaaagtgct gcaggaagta ttcggagaac
     2761 ttcggtttga gatattggat agttctgcaa gaatgaacta tatccgctac cagttaggac
     2821 agacgattca aaaagtgaca tgggctttgc aaaaacagag caaaaataca ggcatgcatc
     2881 ctcttcagac agagattcta tttggtcaga ttgctggagc aaaaggtatc acaggtctgg
     2941 aattaccgtt gagtaatggt ggaaaactcc atgttcgagg gaaaatcgat cgaatcgatg
     3001 tagcttctga acaagaagat acttggctaa gcgtcgttga ttacaaatct agcggacgtt
     3061 cattcgatgt cacagaagct tattatggca tggctatgca attattgacg tatctggacg
     3121 tagcattgat ggatgcggta cgtttgactg gaaaggcggc tgttaaacca gcaggatcat
     3181 attatttaca tgttcataat cctgtattga cggagacaga tgatgtagag aagaatacat
     3241 tgaaaaaatt tagttatgat ggcgtttttg tcgatgatcc cgctctttta gaagtactag
     3301 atcattcact ggaagcaaaa gaacaatcac tgatctatcc agtgaaaaaa aatgcgaaag
     3361 atgaatacca aaaaggtagt ttcagtaaag acaaattttt cacggaagaa gaactgcaac
     3421 tattcatggc gcataatcgg gaaaatatgc ggttagctgg agatgcgatt acttccggag
     3481 aaatcaaact aaatccagct tataaagata aacagcggat tgcctgcgaa ttttgtccat
     3541 tcagaagcat ctgtacgttt gatgtgatgc tgaaagaaaa taattaccac cgtctggaga
     3601 aaatagataa ggaagaagca ctgcgtagaa tattgaaaag gagtgaagaa gagtgaatca
     3661 aattccttta aaaccgcaaa atgaacgatt cacagatgat cagtggcaag cgatttttga
     3721 tcaaggagat aatctattag tctctgcgtc tgccggatca ggtaaaacta cagtgcttgt
     3781 tcgaagagtg attgaaaaac taaaaatggg ttttgatatc gatgaattat tgattgtgac
     3841 atttactgaa gctgccgcaa gagaaatgaa agaaagaatt caagaggcat tacaagaatc
     3901 cgtcaatagc gaaagtgacc ctgtacgtag acagcatttt actaagcagt tagttttatt
     3961 gcccacagcg aatatctcaa ccttacatgc tttttgcctg acagttattc gacgttatta
     4021 ttacttaatt gatattgatc cagtctttcg catgttaaca gatgaaacag aaacgatctt
     4081 gatgaaggag gatgtgtggg acgagctgag agaagcactt tatgcagaaa atgatgaacg
     4141 atttttccaa ttaacaatga atttttctaa tgatcgttca gatgacggct tgacgaatct
     4201 ggtgttttct ctttatgagt ttgcccgtgc caaccctgac ccccaaaaat ggctagaaca
     4261 gttaagcgat aattatcgtt tgcctgaggg gctagcgaaa tctcgattat atcaaaaaca
     4321 aatccgtcct ttagttttgg cagatatata ccaatgtgta cagttatatg aacagatgac
     4381 tcagttagca caaggtgaag gattagaaaa aatgcatgag caagtagctg gagaacaaca
     4441 gcagatcaag aatatctatg aagcgttctc gcaagatcgg ttagaagaag cttatgctgg
     4501 tttggaacaa ctaacttttt caacttttaa gagcagtcgg aaagcagaat tgaaagaaat
     4561 ttccaatgaa gtcaagggca tgagggataa agcaaaaaaa ctgattcagc agataagtaa
     4621 aagttatttt cctgtttccc caagccaaat ggaagaatta acggacaaag cactaccgtt
     4681 agtcgaagaa atgacaaaag tcacgcaaag cttcatggac ggtttcagta tgcgtaaacg
     4741 ggaaaaaggt gtgctggatt tcaatgatct ggaacattta gcgttgcaga ttctcacaga
     4801 aaaaacgaaa gatgtatggc tgccaagtga agcttcaaaa cattatcgaa aaaaattcaa
     4861 agaagtaatg gtagatgaat atcaagacgt gaaccaatta caagaagcca ttttatactg
     4921 gctaagagaa cccgatgata caaaaggaaa tatgttcatg gttggggatg tcaaacaatc
     4981 catctattct ttccgtttag cagatcctag tttatttatc gggaaatacg agaatttctc
     5041 aaagaaagaa ggcggacgtc gaatcgtttt ggctgagaac ttccgctcta gaaaagaagt
     5101 cctttctttt acgaatctta tttttgaaca actaatggat ccagcagtgg gtcaaatcaa
     5161 ttatgacgaa gcagccaaac tgatccaagg attttcagac tttccagaga atgaacagtt
     5221 tgagccggaa atcatgatct atgaaaaaga acaggaagaa tcagagattg agattccgac
     5281 ggatgatata ctggaagaca aaacagaagg cgaacttttt atgacaggac taaaaattcg
     5341 tcaattaatt gattcctcct ttatgattta tgataaaaaa tcaaaaaaaa gtcgtccgat
     5401 agagtacaaa gacattgttc tcttgacacc tacaaagaag aacaacttaa cgattttaga
     5461 gattttcaaa acattggata ttccattaga gatgaatgat gctcaaaact attttcaagc
     5521 gacagaaatt cgcacgatga tctcgctgct acaattaatc gataatccct atcaagatat
     5581 tccgcttgcg gctgtattgc gctcaccgat cgtgggtctg attgaaccag aattagccag
     5641 cattcgatta gctaaccgtg cacatactta ctatgatgct gtgcttgctt atcaagcatc
     5701 aaacgaagat gaattagcag ctaagctgga acattttggc aagcaactag agcattggcg
     5761 agaacttgct cgccgttctt cgatcactga cttgttatgg gatatctatt atgagacggg
     5821 atatctagag tatgtggtcg gtctgccagc aggggcacag agacaagcca atctttatgc
     5881 attggttgat cgtgccaaag cttacgagca aagttctttc agaggattat accagttcgt
     5941 gcgtttcatt gaaaaaatgc aagaaaaaga taaagactta gcagaaccag tcatttcaat
     6001 agaagataat gctgttcgcg ttatgacgat ccatgcaagt aaaggcttgg aattcccagt
     6061 tgtatttttg ttagatatga caaaggagtt caacttacaa gacttgcgca atcggtatgc
     6121 ttttgaagaa aaacttggag caggaattcg ctatatggat cctgaaacac gagtgttata
     6181 tgatacgtta ccgtttcaag ccatcaaatt ggcaaagcaa aacaaattgt tgtcggaaga
     6241 aatgcgtaaa ttgtatgtag gattgactcg ggcagaacaa aagcttttca ttgtcggttc
     6301 ctataaaaat aaagagcaaa tgatccaaac gtggtctgaa gcagctgatc atgaggagtt
     6361 agtttttgat cctgcgctac gtttgaaggg cagaagcagc ttgatgaatt ggattggtta
     6421 cggattgatc cgtcatcctg aaatgcagaa atacttagag gaagaaattt cgacttctct
     6481 tcttcaacat tcaaatgcac agttttctat ttcatggatg aatcagcaat cgatcattga
     6541 acaaagacag ttgcttgctg aaaaagaact ggttaactta gatcagcaga tgaaagaaga
     6601 tgaaacttta ttagcagatt cgttacaaaa acgtctggca tatgaatatc cttatcaagc
     6661 ttcttcacaa accacaagtt atcaatctgt atctgaaatc aaacgattat tcgaagatcc
     6721 agatgataca caagaatcac gtttgactct ggaaagcagt cagaacaaag cagcatcccg
     6781 tcaatttcgt tatacgcaag aacaactagc agaaccaaaa tttttgcaaa aagatcggca
     6841 agtgtcagca gctgctgttg gaactgctac gcatgctttg ttacaattac tgccattaga
     6901 gatgccaact acagagagta tccatcaaaa attacaggaa ttggtaagga aacggttagt
     6961 agacgaaaaa gtcgcgaaaa aagtagatgt ttcttctatt atatggttct ttcaaacaga
     7021 acttggtcaa cagctgattg ccaacaaaga aaacgtaaaa cgagaacaac ctttttccat
     7081 gctgcttcct gctgacgaag tatttcaaga ctatccaaat caagaagatg aactattaat
     7141 ccacggaatc gtcgatggat acctagaaga aaaagatcat ctcaatatct atgattttaa
     7201 aacagatttt attttacatc cggatgatcc agcagaaata gatgcaatcg tccaaaaata
     7261 tcaaggacag ctaagacttt accaacaagc aatgtcagaa gctttgaata agccagtaga
     7321 aaatgttttc ctaattttgc ttcgagtgaa acaaattata aacattaaca aataattata
     7381 attgagcaaa cttgacttat gttttgtaag gattatgcta tactctggga agaggtgagt
     7441 atgatggagc taacacagac agaattcgtt ttatgcccta aatttgaaaa aacattctct
     7501 attttaggaa aaaaatggaa cggattgatt atcgatgtac tactcgaaaa cggtccgcaa
     7561 cgctttgttg agcttgcggc aaaaatcccg atggtcagtg atcgagtatt agtcgaacgc
     7621 ttgaaggaat tagaaaaaga aggaatagtc actcgaaact ttgcctgtag cgaaaagagc
     7681 cgttcagaat atctcttgac tgaaaaagga gcagacttgc aaaaagcgat gcgtcaaata
     7741 caagtttgga gcgaaaaatg gatcacagag gaagaatgcg gttgattttg taaaaaaaat
     7801 cttgtaaact atagtccata cgaataagta aagaacgatg acgaaaaaga gtagtttggc
     7861 taaaccagtt cagggagatc tgtcgcagac tgaaagcaga tcctgtgttt gaaaaatgaa
     7921 gttcactttc cgagttcgtc tgataaaatc agaccggcaa ttgtcgttaa cgaagtaaga
     7981 gtgcggtgta agtcatttat ccgcaaatta ggatggtaac gcgaagacct cgttccttaa
     8041 caggaatgag gtctttttgt tttgtatttg gaatttaaag gaggaacaag cacatgtctc
     8101 tacaagcaca attagagagc ttgaaagaag caacgctcgt caaaatcaca gcggcacaag
     8161 atttaaatga attaaatcaa atacgtgttg aaacgttagg aaaaaaagga ccgatcacag
     8221 aagtattacg tgggatgaaa gatctaagtc cagaagagcg accaaaagtc ggaagtttcg
     8281 caaatgagat ccgagatctt ttgacagaag agatcgagcg aaaaaaacaa gtattagaag
     8341 aagaagcgtt aaatgcagct ttggcaaaag aaacaataga cgtgacacta cctggtagaa
     8401 aagtaaaaca tggtacacgt catatcgtga cacaaatcat ggaagaaatt gaagatattt
     8461 ttgtgggtat gggctatcag atcgtagaag gatatgaagt agaatctgat cattacaatt
     8521 tcgaacggat gaatctgcca aaagatcatc cagcaaggga catgcaagat actttctata
     8581 tttcagacga gatattgatt cggacacata cttcaccagt ccaagcacgg acaatggaaa
     8641 aacatgattt ctctaagggt gctctacgaa tgatctcgcc agggaaagtc ttccgcagag
     8701 atacagatga tgcgacccac agtcatcagt tccatcaaat tgaagggcta gttgttgata
     8761 aaaacatcac aatgggcgac cttaaaggaa cattagaagt cgtaatgaaa aaaatgtttg
     8821 gggaagatcg tgaaattcgt ttgcgtccaa gttatttccc atttacggaa ccatcagtag
     8881 aagtagatgt cagctgtttc aaatgtggtg gtgccggttg taacgtatgt aaatacactg
     8941 gatggatcga aattttagga gctggcatgg tacacccgaa tgtgttgaag atgtcaggaa
     9001 tcgatccaga agaatattca ggctttgctt tcggccttgg accagaccgc gtagctatgt
     9061 tgcggtatgg agtgaatgat atccgtaatt tctatcaaaa tgacttacgt ttcttaagtc
     9121 agttcaaggg ggagtaagaa aatgttagtt tcatataaat ggctcaatcg ttatgtagat
     9181 ttatcgaatg taacaccaaa agaattagcg gataagatgt ctgtgacagg aatcgaagta
     9241 gagggagtaa cggttccaga agaaggcttg aaaaaaatcg ttgtcggtga agtcaaggaa
     9301 tgcgtaccac atccagattc tgatcacctt tctatttgtc aagtcgatat tggcgaagac
     9361 gaactgtctc aaatcgtttg tggagcgcct aatgtgaagg ttggaatcaa agtgattgtt
     9421 gcacttccta actcacgtat cgctggtaat gtaaaaatca aaaaaggaaa aatgcgtggc
     9481 caagtatcaa atggcatgat ttgctcatta caagaaattg gctattcaga tagtgttata
     9541 ccgaaagaat acgctgaagg tatctattat atgccacaag aagctgtaaa tggcgatcca
     9601 gtttttcctt atttggatat ggatgattcg attattgaat tatctatcac accgaatcgt
     9661 gcggatgcat tgtcaatgcg tggcgtagct tatgaagttg gggcaattta tcgtcagact
     9721 cctacattcg atgacgaaaa gctggtagaa tcctcacgtc ctgctagcga taaaattcaa
     9781 gtggaagttg aagataaaga agcagttcct gcttatcaaa tcagaatcat tgaaggagta
     9841 agaatccagc caagtcctca atggctgcaa aatcttttga tgaacgaagg cattcgtcca
     9901 atcaacaatg tagtagatgt gacgaactat attttgcttt tattcggaca accactacat
     9961 gcatttgact acgacaaatt gaaatcaaaa gagatctttg tccgccgtgc aaaagaaaat
    10021 gaaacattag taacactaga tggagaatct cgggaattga caactgacaa tctcgtcatt
    10081 actaacggca aagaaccagt tgctttagct ggtgtcatgg gagggttaga ctcagagatc
    10141 acgaatgaaa caacaacagt tgcactagaa tctgctttat ttgatccaat ttcgattcgt
    10201 cgtacagcaa aacaatttaa tttgcgtagt gaatcctctg cacgttttga aaaaggaatc
    10261 aaccatgcga ctgttggaga agccagtgag gttgctgcag caatgatcgt ttctcttgcg
    10321 ggtggggaag ttctacaagg cgcggtcaaa ggctcagaga tcaaagcaga agacgtatca
    10381 gtgaagattt cacttgaacg catcaatcaa tatttaggaa catctcttgt cgttaaagaa
    10441 gtaaatgaga tttttgaagc acttggattt agctatgaag aaaaggagta tgtttattcc
    10501 gtagtagtac caccaagacg ctgggatatc caaatcgaag cagatattat cgaagaagtt
    10561 gctcgtattt atggatacga ccagttgcca tctacacttc caagtggaga aacggttgct
    10621 ggaagcttgt caaaagaaca agaaactact cgtaaaattc gtacaatctt tgaaggaagc
    10681 ggattgagcg aagcaatcag ttatgcattg acaacagaag aaaaatcaag acagtttgtt
    10741 ataaatgaaa gtaatattac acgtttggac tggccaatga gcgaagaacg ttctgtatta
    10801 cgaatgaatt tgatcagcgg actattagat gatatcagtt ataacactgc ccgaaaaaat
    10861 acagagattg ctttatatga aattggtcgg gtgttcttcc aagaaaatga tccacgtaca
    10921 catctgccgc aagaagtaaa acatgcagca atcgctattt ctggtatttg ggaagaaaaa
    10981 gattggcaga cgaaagctca aaatgtagac tttttcacca tcaagggtct tttagaaaac
    11041 ttatttgaac aactaggcat cactgaagat gtccattatc aaacagtgac agaaatgaag
    11101 gaaatgcatc ctggaagaac tgctgcgatt taccttggcg aaaaatttat tggttttgtc
    11161 ggtcaagtgc atccaacgat tacaaaaagt tatgatattc cagaaacata tgttgctgag
    11221 tttgatttat ctgctatagt ggaagctgct caaaaaggga tcgttttcga agcagtgaca
    11281 aaattcccag cagtcagccg ggacatcgct ttgcttgtaa aagaaactgt gacaaatcag
    11341 gaattaacag aagttatccg ttctgctgcc ggaagatttt taacagatat ccaattattt
    11401 gatgtctatc aaggagaaaa cattgaaaaa ggacataaat ccatggcgta tagcctgaca
    11461 ttcgtcaacc cagaagcaac attgacagat gaagaaatca ataaagcaat ggaaaaagtc
    11521 gaaaaagcgt tggtagaaaa cctagaggca agtattcgct aagaaggaaa atatagtaag
    11581 taacgatcaa gtagcttttg aagaccatac atttgaaaaa aagatgtgac acaacttcgt
    11641 cacatctctt tttttgattt taaaaaagga atagcacaga gacgagataa aaaaacaggc
    11701 ctttacagcc tgctttttta gagtaaatga attaatggaa gtaattgatt cccattgctg
    11761 attttacttc agaaagtgtt tgtgcagcta ctttagcagc ttcttcacta cctttacgta
    11821 agatttccat aacagctgaa ggatcttttt ccaattcttc tcggcgcgca cggatcggtg
    11881 caaattcagc ttccaataca tcaatgaggt atcgtttgat ctttacatca ccaagtccgc
    11941 cgcggcggta atgagctttc atttcttcaa ttgcttcttt atctgttccg aatacatcca
    12001 agtaggtgaa gaccatattc ccttctactt gtccaggatc ttgtacatgg atgtggtttg
    12061 gatcagtata catactcatg acttttttct gtaagacatc ggcaggatcg gagatataaa
    12121 taccgtttcc taacgattta ctcattttgc catttccatc gattccaggt aatctaccca
    12181 ttccttttgg tgggaatacg ccttttggtt ccaccaagac ttcaccatat gtatgattga
    12241 agctttgtac gatttcctgt gtctgctcta gcattggttt ttgatcctca ccaacaggta
    12301 caagattcgc tttgaatgca gtgatatctg ctgcttgaga aacaggatag ataaagaatc
    12361 ctgtaggcac gctttcaccg aatttttttt gttcgatttc tgttttgaca gtcggattgc
    12421 gccgaacgcg tcctacgctg actaagttca aataatacat ggttaattca gctaattccg
    12481 gaatttgtga ttggataaat aaagtagatt ttgcaggatc taatcccacg gctaaatagt
    12541 ctaaagcaac ttgcaagaca tttgatgaaa ccttctcagg atttttagca ttatctgtca
    12601 gtgcttgcat gtcagcaatc atgacaaaca gttggttgtc ttcatctgcc tgcatagcca
    12661 ctcgggtttt tagagaaccg acgtaatgcc ctaaatgcag cttcccagtt ggtcgatctc
    12721 ccgttaaaat aatatttttc aaaaaagaca cctctttacg atttgtaagt ctgagcactt
    12781 aatatcttac cgtttttcct gaaaagaaac aacagttcct aaggaaaaaa caaggaaaga
    12841 ggataaaata actaatagat tagaaatgga agaaatattt atgggagaat aaacgttcta
    12901 agaaacttta caaattacta aactatcaga tataatgtat tttattatac atctttaaaa
    12961 gaaatcttac taaataataa tgggagtgta attctatggc aatgattaaa tttgatcacg
    13021 tggataaata ttatggcaag ttccacgcat taaaaaatat caatctcgaa ttcgagaaag
    13081 gagaagttgt cgttgtcatt ggtccttctg gttcaggaaa gagcacgatg cttcgctgta
    13141 tcaatggatt agagacgatc tcttcaggta agctgctgat taatgatact gacttgcatg
    13201 ataaaaagac aaaactgaca gaagtccgaa aaaacatcgg catggttttc caacatttca
    13261 atttgtaccc aaataaaact gttctggaaa acatcaaatt agcaccaatc aaagtgttga
    13321 agcaagacca ggcaactgca gtcaaaaacg cggagaaatt tttgaaaact gtcaatatgt
    13381 tagataagaa agattcttat ccttccatgt tatccggggg acaacagcaa cgggtggcaa
    13441 tcgccagagg tttagcgatg aatcctgaga tgctgctgtt tgatgaacct acttctgctt
    13501 tggatccgga aatgattggt gatgtattag acgttatgaa aaaattggct catgacggta
    13561 tgtcaatgat tgtcgtgaca catgaaatgg gctttgccaa agaagtggcg gatcgtgtta
    13621 tcttcatggc tgatggagaa gtattggaag acagcagaaa tgtgagagac tttttcgaaa
    13681 atccaaatga agtccgagca cagcaattta tcagtaaagt tatcaatcat tgataggagg
    13741 aaaaagatgc gcaggttaaa aaaaatacgc ctgattttcc ttttaggcat tgcttttctt
    13801 gcactagccg gatgtaaagg aaatgggttg gcaaatgaag atattttaga gcgaagcaaa
    13861 tctaccaatg aaatcatctg gggtgtcaag tatgatactc gattatttgg aatgatggat
    13921 attgagagca gaactgtaca aggatttgat gtagatattg caaaagcaat cactaaaaag
    13981 atccttggtg acaatggaaa aacagaattt gttgaagtca cttctaaaac tcggattcca
    14041 ttattgaaaa acggaaatat cgatgcaatt attgcaacga tgacgatcac agatgaacga
    14101 aaaaaacagg ttgacttttc agatgtctat ttcgatgctg gccaagcttt gttggtaaag
    14161 aaaggaagcc aaatcaaaag tgtagatgat ttgaatgctt ctacaacggt tctagcagtc
    14221 aaaggttcta catcggctgc gaatatccga cagcatgcac cagatgcaaa aattttagaa
    14281 ttagaaaact atgcagaagc atttactgct ttacaatctg gtcagggaga cgcgatgact
    14341 acggataatg cgattttgtt agggattgca gatgaaaatc cagagtatga gttagttggt
    14401 ggaacgttta ctaatgagcc ttacgggatt gcgatcaaca aagggcaaga aaacttcttg
    14461 aaagctgtta accaagcgct agaggaaatg catgcagatg gcacatacga taaaatctat
    14521 caaaaatggt ttccaaatga aactgaagga aaagtagagt aagggggaga ggcaatgagc
    14581 gaattgatac aaacttatgg accaatcttt gtcgacgggt tcaagatcac attgttttcg
    14641 agtgtcttag cacttgtgtt tagtttgatt atcggaacgt taatggcaat cttccaatta
    14701 tcaaagaata aagtagtgag cgccttagca aaggcatatg tagaattttt tagaaacatt
    14761 ccattattaa tcatcgtgat gtttttttat gttgtcgtac cgatttattg gtttaatcta
    14821 gatggattcc aagcaggtac gattggatta acgatctata catctgcttt tattgctgaa
    14881 acagtacgtt cagggattca aaccgtacca aaaggtcaaa cagaagcagg actatcttct
    14941 ggttttacct attcagaaat catgcgttat attgttttac cgcaagcatt caaaatcgtt
    15001 atcccgccat taggcaacca atttatcaac ctcgtaaaaa actcatctat tttagcaatc
    15061 gtagctggtt tagatttgat gtatcaagga gacttgatcg ctagtgaaac attcaatacc
    15121 ttcgatacgt atatcatcgt tgggttattt tacttagtta tcacgctacc gttatcttac
    15181 ttaatggttt atcttgagaa aaaatgggcg gttcgttcat agaaggaggg gaaatacatg
    15241 gattttagcg gcgctttttc atggatgaat atccgttttt tattggaagg attaaaggtt
    15301 actgttgaag tttcattgtt ttcgatcatt tttagtttct tgatcggtgg tctgacaggt
    15361 gtgattcgtt ttgcgaatat ccctttcttt tccaaaatcc ttggtgtagt gattgatatt
    15421 atacggaatc ttccattatt attgatcatt ttcttcactt actttgcctt acctcaaatc
    15481 ggcattcaga tgaacatctt ctggtccgct gtcgcagcat taacgatttt tgagtcagct
    15541 atgttgtcgg aaattttccg agctggactc aatgctgttc caaaaggaca gatggaagca
    15601 ggattatcaa cgggactgac ttatgtagaa tcgatgcgaa cgatcgtgtt accgcaagca
    15661 tttaaatcaa tgatcccggc aattgtgagc caattgattt cactgatcaa agatacttca
    15721 ttagcagtaa tcatttcttt acctgaactt tctcatcagg cacgtattat ttatggacag
    15781 aacaccaatt atgtgcttcc gatgttcatc atcatgacac ttatgtactt cgtggtatgt
    15841 tacgcgcttt ctctgctttc cggttacttg gaaaagagac ggtacagtta ttaatagatg
    15901 attcttttag cggttgtggc aaactaaagt cacaaccgct tttcctcttt ccattcgaaa
    15961 aaaagagatt tcgattgttt taagcaagta agagcacttg aaaattcacg ctaaaaagta
    16021 cacaataggg aggatagaat ttattgaaaa aaaggatgat acgattgaca gataatcgcc
    16081 ctatcggatt tattgattca ggtgtcggcg gcttgactgt agtaaaagaa gccctgaaac
    16141 aattaccgaa tgaaaatatt ttatttgtag gagacacagc acgctgccca tatggcccta
    16201 gacccgcgga acaggtaatc cagtatactt gggaaatgac ggattatctg gtggagcaag
    16261 gaatcaagat gctggtgatc gcctgcaata ccgcaactgc ggtggcttta gaagaaatca
    16321 aagctgctct ttctattcca gtcatcggtg tgatccttcc cggtactaga gcggcagtaa
    16381 aaaaaacaca aaataaacaa gttggcatta tcggtacgat tggtacggta aaaagtcaag
    16441 cttatgaaaa agcactgaaa gagaaagtac cagaattgac tgtgacaagt cttgcttgtc
    16501 ccaaatttgt ttcagttgtc gaaagtaatg aataccattc atcggtggcg aaaaaaattg
    16561 tggcagaaac attagctcct ttaaccacta aaaaaatcga tacattgatt ttgggatgca
    16621 cccattatcc attattacgc cccatcattc aaaatgtaat gggagaaaat gttcaactga
    16681 tcgattctgg agcagaaaca gtaggtgaag tatctatgct gttagattat ttcaatttga
    16741 gcaattcacc gcaaaatggt cggacattat gccagtttta tacaactggc tctgccaaac
    16801 ttttcgagga aatagctgaa gactggcttg gaatcggaca cttaaatgta gaacatatcg
    16861 aattgggagg aaaataagga tgcgtcatga cgggagaaaa gcccaagagc ttcgcaaact
    16921 ggagcttcaa acaaatgttt tcaaatatcc agaaggatcg gtagtgatcc gttttggtga
    16981 tacaacagtt atttgttctg caacaataga aaattctgtt cccccttttt tgagagaaac
    17041 aggaacagga tgggtgacag cagaatatag tatgctgcct cgagcaacga gtacgagaaa
    17101 tcgtagagaa agcagcaaag ggaaactttc tggtagaaca atggaaatcc agcgtcttat
    17161 tggtcgttcc ttgcgtgcag tagtagactt ggaaaaatta ggagaacgta gtatcatcgt
    17221 agactgcgat gtgatccaag cggatggcgg tactagaaca gcaagtatca ctggggcatt
    17281 tgtggcattg aaattagcca ttgaaaaact tttaagagaa aaagaattgt cagaagatcc
    17341 aataaaagaa catcttgctg cagttagcgt aggaatctta ccagatggga cttgcgtcac
    17401 agatttggat taccaagaag attccgcagc acttgtagac atgaatcttg taatgacaga
    17461 atctggaaaa ttcgtggaga ttcaaggaac tggagaagaa gcaacgtttg atggtgaaca
    17521 gttgaatgaa atgctctttt ttggaaaaaa cgcgattgaa gatttgatca aagagcaaaa
    17581 acatgctttg ctaacagagt ttgctcaaaa tgatgagcgg atagaagaaa caaaaacaat
    17641 catcattgcg acaagaaatc caggaaaagc agaagagttt cgaaatatgt tcaaagaagc
    17701 gggatatcat gtaaaaacat tacttgacta tcctgaattg ccagatgtgg aagaaactgg
    17761 cagcacattt gaagaaaatg cccgattaaa agcagaaacg attgcacaat tgcttgatca
    17821 gccagtctta gcagatgatt caggtttgaa agtggatgct ttaggcggta tgccaggaat
    17881 ctattctgca cgttttgcag gggaacaaaa aagtgatgcg gggaataacg ccaaattatt
    17941 atatgaattg acagatgtgc cggatgaaaa aagaacagca caattccatt gtacgttagt
    18001 attcgcggca cccaaaaaag acagccttgt agtagaagcg gaatggcccg gtcgagtagc
    18061 ccgtatacca tctggcgaaa atggctttgg gtatgatcct ttatttatcc cagaaggtaa
    18121 gaaacaaaca gcagcagaat tatcgagcga agaaaaaaac aagatcagtc atcgggctca
    18181 agcaatgaaa aaattgagtg ccgagtggaa acaatggctg gaaggagaga gatagatgcg
    18241 gtatttagta gtaagtgata atcatggaga cagagatatc ctcgttgact tagtagatcg
    18301 ttatcgttct gaagtggata tctttttcca ttgtggtgat tcggaattag aagcttctga
    18361 cccattatgg gatacattcc aagtggttca aggtaattgt gattatggac caggattcga
    18421 acagaaaaaa gtgatccaaa ctggtcagga tacagtattc atgactcacg gtcatttatc
    18481 gaatgtccga tttggtttga cacagttagc aatcgaagct cgagctgcaa ctgccgatat
    18541 ggtgtttttt ggtcataccc atcaaatagg ctgtgagatg gatcagaaaa tcctgttttt
    18601 gaatcctggt agcatttctc agcctagagg cccgatccaa atcccatcat atgctcttat
    18661 cgaaagttta gatgaccaaa tttctgttca atactataac cgtgcccatc aggcagttga
    18721 agagttggcc ttttcttttt cgagagagcg ctgaataaaa aaactctaat ttttaataaa
    18781 aataagcttt attttatgaa ttttgcatcc ggtttcggta aaatagtagt gttgaatgaa
    18841 atagaaatgg aggccatcag atgattggac ctatagtaag agaaatgctg ctggaaaatc
    18901 aggaaacgtt tttgattcct gcagaaaatg tggcaaatgt tatgtatcat catccattgt
    18961 cccatggttt acttgtgtta tcgaaggtcg gttattccaa aattcctgta ttaggaaaag
    19021 atgatcactt tgtaggacta gtaagtttat cagatgtggt caataaaatg attgacttgc
    19081 aaacaatcag catggacccg ttagaaggac taacggttgc tgatgtgaca gaaacagatg
    19141 ttccgacaat tgatgaaaat tgggaactag aagaagtact ccatttgtta gtcgattcgc
    19201 cttttttacc ggttgtcaca gaagatcgga ttttcaaagg aatcattaca agaaaagagc
    19261 tgcttaaagc cgtaaacttc atggtgcatg aattagaacg tcgtaatata ttgtctccga
    19321 aaatcgaaga attaaaagaa caaatcgata tcgtgagcta aagaacgaga aactaaaaga
    19381 taatcaaaaa ataagtagaa aaaacagcgt accagttttg actttatctt gaaaaagagg
    19441 ctgggaca
//
LOCUS       DACEUZ010000042        18980 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.042, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000042 DACEUZ010000000
VERSION     DACEUZ010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18980
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.042"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(330..647)
                     /locus_tag="IXG44_001287"
     CDS             complement(330..647)
                     /locus_tag="IXG44_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ1658019.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            complement(648..902)
                     /locus_tag="IXG44_001288"
     CDS             complement(648..902)
                     /locus_tag="IXG44_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ1658020.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDTIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            complement(1162..1380)
                     /locus_tag="IXG44_001289"
                     /pseudo
     CDS             complement(1162..1380)
                     /locus_tag="IXG44_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            3193..4233
                     /locus_tag="IXG44_001290"
     CDS             3193..4233
                     /locus_tag="IXG44_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HAQ1658021.1"
                     /translation="MTDFNFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCSVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESGISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYE
                     SSPSTITKDWLE"
     gene            5057..5389
                     /locus_tag="IXG44_001291"
     CDS             5057..5389
                     /locus_tag="IXG44_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HAQ1658022.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIS"
     gene            6008..6349
                     /locus_tag="IXG44_001292"
     CDS             6008..6349
                     /locus_tag="IXG44_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HAQ1658023.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     NEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSV
                     EIIEFVADKEE"
     gene            6368..6658
                     /locus_tag="IXG44_001293"
     CDS             6368..6658
                     /locus_tag="IXG44_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1658024.1"
                     /translation="MKFFEVKSPYYYALIAAKNEEECAELYEQVVTDIENREEFIRCL
                     HELNRNEAIQEDAKKISEETLQPIGMEEATKDIFTIIDEQKSCVLSIDSTLV"
     gene            6850..7083
                     /locus_tag="IXG44_001294"
     CDS             6850..7083
                     /locus_tag="IXG44_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1658025.1"
                     /translation="MARPVLDAMDMDTLTKGRYVQLLARGYSPKDVFKALSKGTDMEK
                     ERLRKEFDYWREHNGIKDLKPARPVIRRKKLKK"
     gene            7503..8174
                     /locus_tag="IXG44_001295"
     CDS             7503..8174
                     /locus_tag="IXG44_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAQ1658026.1"
                     /translation="MKKWLFGFLGVALIVFCSVFGYVSYQKHEGDVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTCKFIVSEYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            8227..9855
                     /locus_tag="IXG44_001296"
     CDS             8227..9855
                     /locus_tag="IXG44_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAQ1658027.1"
                     /translation="MAINRYVKKAGMLVGMLGIIAGCFQGYRAYAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PREDSDLTNAKVDEIVKDVEDKGSNSEYVKNATNKITSAVDENGEITFADQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDNTGSGFLKDIHLYPKNIVSKLSFELTK
                     FGDDGTAQSKQTPLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDKEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGADGITVNGVKSETSPYSVFKWGDTA
                     GQGLSYVAEKANIKVTNNDGSVVLKENTDYKIENSDSGFVIDFIVNNGQVSDTVANLH
                     GQDLKMTYNMFVNDSAEIANPLTNSVDFVYSNNPFNQEEHHEKTKADAVTYGAKFLKA
                     DGGLFGTGIKATPLEGAEFAAKNAEGKYYSFCGR"
     gene            10364..10765
                     /gene="tnpA"
                     /locus_tag="IXG44_001297"
     CDS             10364..10765
                     /gene="tnpA"
                     /locus_tag="IXG44_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003378761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HAQ1658028.1"
                     /translation="MDNEYRYSKTTVSLVNYHFVFCPRYRRKIFVNSEVDARFKALVQ
                     EIAKENEWNIIAIETDKDHCHLLINVQPTYSPSKIMKIIKGKTSSIIRQEFEHLSKMP
                     SVWTRSYFVSTAGNVSSEIIKRYVENQRTRY"
     gene            10789..11907
                     /gene="tnpB"
                     /locus_tag="IXG44_001298"
     CDS             10789..11907
                     /gene="tnpB"
                     /locus_tag="IXG44_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAQ1658029.1"
                     /translation="MKKAHKIRIYPNCRQEIQFQKTFGCVRFYWNFLLDQRITNYKKK
                     KENADYKEDKTTYASLKKMEEYAWLKEVEAQPLSQVAMDLNKAFKNTFNSTFGFPKFK
                     SKKYSKKSYRTAMGIKVNRNYFYVSKVGWVKMAETLRFNGKLMNVTISQSKTEKYFAT
                     FLVDIENFQKDPVTESIGLDLGLTHFCITSAGEKIGNERFYRSLEKRLVVEQRKLSRR
                     LEVAKKHNRKLEECKNYQKQRVKVARIHEKIFNQRNDFLHKLSSRITDENQIICIEDL
                     NVTGMVRNHKLAKSIADVSWSEFVRQLTYKCEWKGRTLVKIDRFYPSSQICSSCGHND
                     GKKELDIREWTCSNCGTNHDRDINASINIRTEGLSGIA"
     gene            11973..12338
                     /locus_tag="IXG44_001299"
                     /pseudo
     CDS             11973..12338
                     /locus_tag="IXG44_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
     gene            12351..13106
                     /locus_tag="IXG44_001300"
     CDS             12351..13106
                     /locus_tag="IXG44_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ1658030.1"
                     /translation="MIIRKPPKRRVMHKLMIFFWLLSAVAVYLIPVVETNVAKFQQKQ
                     RILAIENLEKTDSSLDVAKIELGDPVGVLTIPSISLKLPIYDGTSEKILENGVGITEG
                     TGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGQKFFIKVNEEQHAYQIYRIEEVK
                     AEELQKNYLDYLMPEKDQDRITLMTCTPKGVNSHRFLVYGERVSFSKEDLKLQKKAKN
                     RKSISLDWRLALLFAFFAFLLFLFIKKIRRRSE"
     gene            13103..13930
                     /locus_tag="IXG44_001301"
     CDS             13103..13930
                     /locus_tag="IXG44_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1658031.1"
                     /translation="MNKRKWLFVCVAALFATAAYSQISFADSSTTPTTSQTTDSSTTA
                     TSDTDSSKNTAEETADSSTSSTESAESTSESSSETSEKLQEVEDTQVIKQNIHIQKII
                     SSNRINNQGRQVAHQEGVNGAKFIVYDITNILEEMTQDDNEKNQSVEIIESKLKDRAK
                     KLSYDQLKKVTEGETKTIDDQEGVIDFSIEVPANKKQAYYIVNESSPENISNSEDIIL
                     LTPISDEKGEFLTDVWLYPKSKKSEPKEEIKKVVSTGVKKNFFDNCWDFFTHLFFND"
     gene            13940..14188
                     /locus_tag="IXG44_001302"
     CDS             13940..14188
                     /locus_tag="IXG44_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1658032.1"
                     /translation="MKIIKMLAFNLSFLAATILCCGLVFSQLSIYPRYQRLFVHFLNI
                     QHASMPLEVINMLLINPIFMASLFLLAGLVTWVLGKKE"
     gene            14219..16330
                     /locus_tag="IXG44_001303"
     CDS             14219..16330
                     /locus_tag="IXG44_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAQ1658033.1"
                     /translation="MEGIMENKIHCTTGRVFTLVLFLFLLLFARGTAVQALEKVDPSQ
                     IRENNNLVLYHDSDSLRNIEVTGINGFKILDEYSTKKQKVLAWPKVKDLKSTSKIVAH
                     YQKVGTYLGKEIDCDVTFSNFVAHAGVNQDGLAKTINGKKYYNYLLFDKNIYRGFFMQ
                     SINVMNVKLDFKYSGSKTKVALSEPQPEINSKMRSMRAAGIELNDTFLSIYSLNGQYQ
                     QRVSWYNDNNESVGERNIDMREFVGYEKMGSLPYFVTTDTACAEYKAPALSTNNLTSK
                     VVGGRAEKAYSSNDHFTDVVGADTYHRSGVTYTLQGASPTFMIGANTNSMMFSFDTAL
                     LWTPQPSKPTKEVFNKANTEEAAHNIDKKVIPQGSDVYYHIHQKFDALTVNTMNKYKS
                     FKITDTFDSKNFDMVSDGKNYDGALWYVEANGTWKKADTGGKLTVSGNTVTYTASDSF
                     LDNAANYGRDYILQLHMKAKNDAKGIAPNVATVTFNNDPNKGIQKTNTVENYFSLKPN
                     KKVEQDGKDVDGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDIASDHYKQFSIQD
                     PVEARLTYKEGSARVIDMSTGKDITSEGTLTYDSNLRTLKWEASADFLSKNLLDGREI
                     QLIFTAKTPLQSEKNIDNQAVAAVENVSNKTNVVTIGVDPNLPQVIVPRTGSTHLVTI
                     LVVSLVLLVLVTLSYVALKFN"
     gene            complement(16366..18417)
                     /locus_tag="IXG44_001304"
     CDS             complement(16366..18417)
                     /locus_tag="IXG44_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HAQ1658034.1"
                     /translation="MKLLNPKTVLAIGTFAATLGFAGSADAASTNVMYRLYNPNNHEH
                     FYTSSTKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVKSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNANKVLNDSKAT
                     QKQVDSAKNNLDSAYKGLKKKPAPTVNKAELQALYNRVKSTAKGDFTEESWNDFQTAL
                     SNAKKVLDDSKASQAQVNNAKNSLDAAFKGLQHKPETPYMVTINHIDNQTGQVIATDQ
                     AQVTSGSTYTAKAKAFKYSEGAKDNFSYKVIGNDTQSQTITGNTTITFNYNQVHQINL
                     LCNNNYKNEGRVDILKEQIINVTHGESVTLTAPAIQGYVLDDRVEPTNSVTLNNVTES
                     QNYIFNYTRKFNVTVNHVNADTNAVLSSESKTVYEGDSFSTPWKNMTDQNYFLCRNDD
                     PSISVDKNGQRVINNVDQNRTITFKYKNISLSDLNNYVRQKELVWLNNYRQQNGAAPM
                     QFNDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENINSFYLYVDEI
                     LSDTGAVNAMIMWKDSAAHNANLLYNNQSIVGLSHAYEVSADGSLSSDNVFISANPIF
                     K"
     gene            complement(18537..18746)
                     /locus_tag="IXG44_001305"
     CDS             complement(18537..18746)
                     /locus_tag="IXG44_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1658035.1"
                     /translation="MYADKKKIEWLLFDSGKSVRSISIESGVPLTTVSDLANKRSSID
                     NMRFINAIKLNKLAEETIDTNASTK"
BASE COUNT         6713 a         2917 c         3545 g         5805 t
ORIGIN      
        1 aaagttacgt caattttgat taaaaatata ctacttgaac ctctcatgag taaagtcacg
       61 aaatttttgg gaatagagcg tacttgatat cttatttttt cactaatagc agtgtctctt
      121 attcaccaag cgatccccgt agttcctacg gttcttaatt tttctctaga ctaatgattg
      181 tatctttaga ccttcggcca taacatggag caaaataaaa aggtactaag tcttatatat
      241 caatcttcga gtaaccgacg gttaacaacc atatcgagcg gcattaatca cccacctgta
      301 gaggatgagc gacttttgcc taattattgt taaaagccta gtttcatttg tttagattgg
      361 aaaatacgtc ctacaagtat ctcttttttt tcatggtcga tccgataaaa taaggcatag
      421 cttttgccga tcacgatccg atatgttggg atatccattc catagatttc ggaaacttcc
      481 tcatgcatag ttggaaaatt tttggttagt tctaaagcct tctggatcgc acctacaaat
      541 ctttttatgc tggctggaga cagagcaagc tcattttccc attcttctat tatcgtttca
      601 aggctggtct ggaaactttt agaataaact agcgtgtact ctttcatcta ttcactccag
      661 ccgtattttc cgaaagcttt taccatttca atttctgaaa ctgttaaacc tttctcaaag
      721 tcttggaaac tttcttcaat caatttgcga tcgtgttctt ttgctatttg agtagtatct
      781 aatatgaata agttgccttc ttgtttggct tggatagtgt ctccgctttt taatccaaga
      841 gagcttggaa ggatcactcc cacgctattg ccgatctttc taacctttag ttcttttccc
      901 atgttatcac actcctttta gtttttgata aataaattat aacatgttat aacaacaatt
      961 accaacggtg tttcttaagg gattccactc catattatta ttggacaaaa taagctactt
     1021 agaacgctga aaacatatac aaacgatatc aaaatacgag tgaactactc accatacact
     1081 tccgttaaca tccatttaag taggtatttt tattatatat agatttaaca tatgtcggtc
     1141 ggttgattga tatagctaaa gttataactc aaccgaagcc gatttatcta cacgaataaa
     1201 gttgcgagtg ttctcggcta attcataaaa attgttatgt gttccatata gcaatactgg
     1261 aataacttta ttttcttgaa tctcagaagc aactctatac acggtgaccg catacttaaa
     1321 tccattagac caattacctg ataaacctcc agttttttgc tcttacttaa atggatcaat
     1381 agaaatttca tcacaaacct atagaggatg agtgacttct gcctaattac gttaaaaaat
     1441 attgaaagta actctatgta gcaaaaaata tctagaattt catttcctca aaactgatcg
     1501 atattgtatc caaaaatata tactacctaa aataggtagt atatattaaa atatgtgctt
     1561 ttgttagaaa aatcaataca taaattatca tatctgtata tgtcatttag gtattttagt
     1621 tatgttataa tagttttgct gtctgctacc ccggcctaat ctggcaaggg gaggaggaga
     1681 tataatgaaa tacctttttg attttgtttt tgcaatcaag ccatttgcag aggtaactgc
     1741 acactacatc tgcaagtggc ttgattgcaa aattgccaaa aaagacagta agcctacccg
     1801 ttaattctat aatatagaaa cgtaaaaaaa aaccccatca gcctgcacgc tgatggggtt
     1861 tggtgatata atgaaatacc tctttgttac tcataattat accataatta gtcacgaatg
     1921 caaccaatag aaaaaactac ttagaacggt agatcatcgc taataacaat gcattgtcca
     1981 ccaaatggat tttgtgaagc attaaataca ttgttattaa ttatcgctgc ttttaactag
     2041 gcctctcccc ttctattata aaagaagata tatatcattt cttaattacc tactacctta
     2101 aaccaaaatt taagtactac ctaatttaag tagtacttaa ataatctaaa aaatagttat
     2161 cccaattgtt atgattttat aatacgatta tttgtaacaa aatgaatatt atataattaa
     2221 acactaccat atttaggtag tgtttaatta ttagcaaaac aaaaaatatt tattctattc
     2281 tcttaacttc aactaagtta tgctgcacat ctttatataa atcatatgca actcattagc
     2341 tagacaaact acaagcatgt agttttatct gttaattatg tactacctaa ataaagtagt
     2401 ttataattaa caagtatata tatttaaaaa tttaaaatta atttttttat gacttgtaca
     2461 aatttatctg atattctctt attaagaact acctaatttg ggtagttctt attttttaca
     2521 ttttaagtag tatttaattg caatttggta gtattttttt attatcttat tcctagaacg
     2581 gtagtactta atataaatat aggtagtatc taatttaagt attaatggta gtatctaatt
     2641 taacaggtag tatgtaacta ctttaaagta atttttttca gattaacaca gtagtatctt
     2701 attaaataag ttaattctaa aggtagtaca taattattat atatatctaa cattggtagt
     2761 acttattgta ttttttagta gtatataatc tattgttttc tagcctaaag ccttgtctta
     2821 taagaataat ataatatata ctactgaaat aaggtagtat atattactag tagtggtagt
     2881 atgttttata tgcataaggt agttcttaat ttattttttg ttttgatact ggtagtactt
     2941 aattatcctt aattttaaaa ccaaatttta ttcatacgtt cttctggtag tatttaatta
     3001 taaataatat ttatttaaag tagttcttaa tgactttaaa ggtagtacat aattcatttt
     3061 atttcttatt ttaggtagta tttaattaaa aaacagtttt aatgcgtttg cattttttat
     3121 ttatctttgc tattttcgaa aagagtaggt aattttacat aaataaaaca acgtgaaagt
     3181 agaggatcta caatgactga ttttaatttt tttaaggcgg atagagttta caacgaatta
     3241 ttttatcaat ttccaaaagt ctttattgtt tctgacgaat acaaaaaaat gaaagattca
     3301 actaagattg cctatatgct tttaaaagca agattagaga tcgcaatcag caaacggcaa
     3361 atcgatgaag aaggtaatgt ttattttact tatacgacaa atgaactatg tagcgtatta
     3421 aactgccaaa aacaaaaagc gatagcaatc aaaaaagagt tggaatcctt tggtttatta
     3481 ttacaaaagc agatgggatt taacaaacag ttagggaaaa ataatcctaa tagactatat
     3541 ctagcagaat taaaagtctc agaaaatgat atctacttac tcgaaaaatt tgatagagag
     3601 aatagggaaa atgttgataa atcagagggt atgaaaatca tacccaccct cgacgaaaaa
     3661 tcagacgctg aatcccttgg ggctcaagag ggtatgaaaa tcataccgtg ccaaaacgtt
     3721 gataaatcag agggtatgaa aatcatacca gaacttaata ataatatatt agacactaat
     3781 agacacaata tagacactga aaaagaccgc ctacaagatc aattgttgtt agacaatttt
     3841 gagacaatta tgacaaacga cagcattgct acgtttgtcc ctgaacgagt attaaatttg
     3901 ataaaaacat tttcttcaag ttacagtgaa gctcaaaaaa ccgtccagac tattcataat
     3961 gcaaagaaaa aagctgaaat agaaagtggt atttcgatag tttttgaaga actcgatagt
     4021 tattatgtca atgcagaaca agaattatac acgacactgt taaaagccta tcaaaaatta
     4081 aaaaccgaaa aagtcgaaaa tatccagaac ctgatttttg tctatgtaaa aaattggttt
     4141 atcgaaaaac caatagctgc taaagtatca agtgaaaaac gtttgaatta tgaaagctcc
     4201 ccaagcacta ttacgaaaga ctggttagag tgaaacttta ctgacaaagt gattgttgct
     4261 tcatggagta acattaaaat taaaaatttt ttattattct aattatatta cgaatattac
     4321 aaaataatag ctattttaat agaatttata agttaaaata ttatgataaa aatcacaaac
     4381 gtagcctttt acgtcataag tgttaaaaga aataattaat ttcaaatata gaaaagggaa
     4441 cgtatgtttg catactcctt ttttttgagt tataataata tgcgaagaga gataatttca
     4501 ttatttaaac aagctaaaaa tcttgatatt acttcttttc aggcaatacg aatattattc
     4561 gcttacattt tttgtataaa tttaattgtc tgctatgctt gtgatgaatt gattttcgtt
     4621 ctgcactttt ggatgataaa tttggacaat tgaaaataca aaaaaaagaa aggcggtatg
     4681 aactcggaag gctggtaacc ttcgtcagcg ttcatacacg gcattaagat ttggccctta
     4741 atgcgttaac ctttcagtgt tacggtatta cccgcaatgg atttagtata acagatattc
     4801 aaaagtgata ccactttttt tggctgtgtt aaggttaaaa atacgtgata ttacgatgaa
     4861 tctatgccta agcataatgt tataaaaatg tccgtgtggc tctttgccgt atgacgaacg
     4921 ctgaaattag agggaacgca tgtttggatg cgttccctct tttttgtttg tcatgaacga
     4981 aaatcaattc tctatttttg aaaaataaca agaaaaactt gttaaaaata tttaaagaaa
     5041 ttgggtggtt tattttatgt catatacagg aattttgagt ctcaaagata tttgtcacta
     5101 cggtaaacgg tgtaccgcta cagagaaaat cacgaagaaa ctatcaactg gccaaagtaa
     5161 aaccgttgtg cagtgtaaga aatatataat ccagaaagac aaagtctcag aggaaatgat
     5221 ttattacatt gggaaacaaa agcaaataat tttaaaagat cctatcccct taaaagagct
     5281 atatccaaca atcaaacatg tttatgacca aaacggtgta ctgattggtc gaagaaaaaa
     5341 tggagtcttg cgttgtaccg caaaagggat ggggcgttta atcagctgaa ttgatcattc
     5401 tcataacttg tattttatcg tttttcgctt gcttgaaaat attgtcgaga cttctaaaag
     5461 aacttagaat cattagaaaa ataaggagta gacttgatct atcaacaatt cccttcgggg
     5521 agtgacgggg taggttttaa cgattggtaa cgtaaaaaat tggtcaatcg agtaaatgcg
     5581 cttgtttgga tagtggtgaa atgaatttgt gtaaataata agcgcagagt aaacaagagg
     5641 gaatttagaa gttagacaag ttagaatagc gtctagatta atcatgagca gctactaagg
     5701 gatctaggct ttgacgcaaa ctcatggaat aaggagtagg tcttctcgta atggggagga
     5761 cgcacaagaa attgtgtcgt gtgctgcagt acatgtagat acttgcgagg acgttggtca
     5821 ttggatcaat cgagcattta ccgctgtcta ctagggtttc tcgtttcgga cgccataaac
     5881 gagtcgatat tttttatgta tttttaagta aacgaaaaac gataaaatat gaacgagtcc
     5941 tgaataaagt gaaaaaattg atataaaaca tagagaaaat gaaatttacg taatggagga
     6001 atgacaaatg aaaaattatg atccgaatat acgattgggc acacatacta ttaaggtttc
     6061 tttccaacgt tgggattaca aaggttttgt gacgtttaga agaggcggaa attgtaaagg
     6121 attagatgtt ttagcattaa acgaggatga tttatacgat caaacattga ctgataatcc
     6181 aattggattc ggcttgctac ctgaagatga tgaaggtaat gaatggttta aaatgacgct
     6241 tatgaatgat aatggagacg aattatctgt tgaagatact tggtcatatt tgagtgatta
     6301 tattgtcagc gttgaaatca ttgaatttgt agcggataaa gaagaataaa aactggagga
     6361 ataaattatg aaatttttcg aagtaaaatc accatactat tatgcgttaa tcgcagcaaa
     6421 gaatgaagaa gaatgtgcag aattgtatga acaagtagta acagatatcg aaaatcgtga
     6481 agaatttatt cgatgtttgc atgagttaaa tcgaaatgaa gcaattcaag aggatgcgaa
     6541 aaaaattagt gaagaaacgt tacaaccaat cggtatggag gaagctacaa aagacatttt
     6601 tacaattatt gatgaacaaa aatcttgtgt attaagcatt gattctacat tggtttaata
     6661 gatccaagat tctataattt ttttcaaagt aaaaagaaat taggtaatga ttctcagacc
     6721 aattacttag gaaaataacc atacaaatta gattttacag ataaaggatt agaaggaatc
     6781 acggcgatta ataaaaaggt agtattacct taggcattga gtaaggaatt tatttaggag
     6841 gaaaattaga tggcgcgacc agttttagat gcgatggata tggatacttt gacaaaagga
     6901 aggtatgtac agctgttagc tcgtggttat tcacctaaag atgtattcaa agccctatct
     6961 aaaggaacag atatggaaaa agaaagatta cgaaaagaat tcgactattg gagagagcat
     7021 aacggaataa aagatttaaa accagcacgt ccagttatta gacgaaaaaa attgaagaag
     7081 taagaccaaa gcgtatggcg tacataggct tttttatttt gataaaaata atgaaaggaa
     7141 ggaaaactca tgaattgtaa agaaatcgaa aatagaaaaa aagtgtcgaa agaaatggaa
     7201 gaaaagttac taaaaacaat gaaacaaaaa caatgaaaca aaaacaatga aacaaaaaca
     7261 ccttaaaaga ttatctgtcg cgcaatatat caatgatatg cagttaacag gaaaagagaa
     7321 agcttgtttg ttaggttcaa tgaaaaactt tgaacagttg agacgaactt atgtgaaaaa
     7381 aagttctaat tgtcaactat tgttagaagt ctcatgacat tcattaatag aattcatttt
     7441 taaaactttt tctatggaaa tgagaaaaag tttttttgtt ttgtctgaaa ggaggcagat
     7501 ggatgaaaaa atggttattt ggttttttag gagtcgcttt aatcgtcttt tgcagtgtgt
     7561 ttggctatgt tagctaccaa aaacatgaag gagatgtgtt taagcaaaat atagaaaaaa
     7621 agatgcccgt agatcaaatc aatgcacacg caaagagtta taaggaggat gcaacgaacg
     7681 tcaataatga tatgtctttg gggcaaatgt tgtccataca aaaagaagca atcgaaatgg
     7741 gcgtcaataa acaagtattt gcacaaattc agattcctgc acttggttta gcattgccga
     7801 tttttaaagg ggcaaatcaa tacactttga gtcttggagc ggctacttat ttttatgagg
     7861 atgccgaaat gggcaaagga aactatgttc tagcaggaca caacatggaa atgccaggtg
     7921 ttttattttc agatattcaa aagttgtcat taggggaggt gatggactta gttagtaacg
     7981 atggcgtcta tcgttacaag gtaacgtgta aatttatcgt atctgagtac tttaaattaa
     8041 ttgatggcgt tcctgaagag aactctttct tgtctttacc aaagaaagga gagaaaccct
     8101 tattaacgtt gtttacttgt gtttatacgt cacaaggaaa agaaagatac gtggtacagg
     8161 gagagctaca ataacaaggt tgtaatcaac gcgagtcgtt gaaatatgaa atgaggagag
     8221 taaaaaatgg caataaatcg gtatgtaaaa aaagctggaa tgcttgttgg aatgttagga
     8281 atcattgcag gctgctttca gggatatcga gcatatgcag aagatgtcac gcaaaaaacg
     8341 ccaccagaga aggtcaatat cacggttcat aaattaatgt acgatcaagg aacgcaattg
     8401 aatgttgata ttgacgggat caaaaatgat ggttatacgc acgatcaata tcctacaggt
     8461 gtaacgaaat acaacaaggc agattatggt gatgttgagt ttacgttagg aaatattacg
     8521 gatcaagtat taccgaggga agattctgat ctaaccaacg cgaaagtgga cgaaatcgtt
     8581 aaagatgtag aagacaaggg atctaacagt gagtacgtta agaatgcaac caataaaatt
     8641 acttcagctg tagatgaaaa tggtgagatt acttttgctg atcaaccagc ctatgtcaat
     8701 tccaaaggga atgtatatgt ggtttatgaa agtaaatccg ctgctggatt agtcacacaa
     8761 aaagcaaaac caatggttgt gattgcgcca atgactgata atacaggttc aggtttttta
     8821 aaagacattc acttatatcc taaaaatatt gtttctaagt tgagttttga attaacgaaa
     8881 tttggtgacg atggcacagc tcaaagcaaa caaacacctt taaaaggggc aaaatttgag
     8941 ttatacaagg gcgaacctgg aaaaggaaca aaattaggag atcttgtatc ggatgatcaa
     9001 ggaaagctga ctgctactga tttgacgtta ggaaaatact attttgtgga agttccttca
     9061 gaagttgtag tgggatcaga taaagaacca acagcagatc aatatttatt aggcgcagac
     9121 gctcgcaacg atgcgcacaa taaattaacc tttgaaatta cgaatgatgg cgtgacaagt
     9181 gacttaaaag cttcatacgt aaactataaa gcaccagtga tcgataaaac agtaacaaat
     9241 ggaacaggac aagaacattc tttccaaatt ggtgacgcag taaattatca aggaacgatt
     9301 catatcccaa cagacattgc aggcggagca gacggtatca cagtaaacgg agtgaaaagc
     9361 gaaacttctc cttacagtgt atttaaatgg ggggatacag caggacaagg actttcttat
     9421 gtcgctgaaa aagcaaatat caaagtcaca aataatgatg gttcagtcgt attaaaagaa
     9481 aatactgact ataaaatcga aaattctgat agtggttttg tgatcgattt tatcgtaaat
     9541 aatggacaag taagtgatac agttgcgaat ttacatggtc aagatctaaa aatgacttat
     9601 aacatgtttg tgaatgatag tgctgaaatc gcaaatccat taacaaatag cgtggatttc
     9661 gtctatagca acaatccatt caatcaggaa gaacaccatg aaaaaacgaa agccgacgct
     9721 gtaacttatg gcgcaaaatt cttaaaagct gatggcggac tatttggaac aggaatcaaa
     9781 gcaacaccgc ttgaaggcgc agaatttgct gctaaaaacg cagaaggaaa atattatagc
     9841 ttttgcgggc gataaaaccc acggttttaa ccgtgtggat gatagcccgc actgtactgt
     9901 aagagcatag ggaaacttgt gcttagtgga atatataaaa tctcgtatat cccaacaaag
     9961 aaactgaatt gcggggaacc cctaaagcga gtaaaacgac aacgtagtct tgaaataggc
    10021 aaacgtgacc gtggcgaaag cagaaaaaat ttactcgata gcatatggtt aaatcctaag
    10081 tgttgcgaca atgggcaatc tgcagcgaag actcgaacag agtaacgttc aacgactatt
    10141 cctcttgagg gaagtacaca caagcgtgtg gaagtggttt cgccgtactg ggtaaagcca
    10201 acggataaga tatagtctgt gctggcacga aagtgtcaga agttcatcag agaactgcat
    10261 aggaagtagc gaacctatgt gaacaaactc cacataaaaa attcttcatt cttttgtgta
    10321 tattaacatt gatttagtac atggcttaac ctaaaattaa ggtatggata acgaatacag
    10381 atactcaaag acaactgtat cgttagtcaa ttaccacttt gttttttgcc caaggtatcg
    10441 tcggaaaata ttcgttaatt ctgaagtaga tgctagattt aaagcactcg ttcaagaaat
    10501 agcaaaggaa aacgagtgga acatcattgc aatagaaaca gataaagatc attgtcattt
    10561 attaataaat gttcagccta cgtattctcc ttctaaaatc atgaagataa taaaaggtaa
    10621 gacatctagc ataatccggc aagagtttga acacttaagt aaaatgccaa gtgtttggac
    10681 acgttcatac tttgtttcaa ccgcaggaaa tgtatctagc gaaattatca agcgatatgt
    10741 tgagaaccaa agaacaagat attgattgcg ttaaggaggt gtgcagaaat gaaaaaggct
    10801 cataaaatca ggatttaccc caactgtcgg caagaaattc aatttcaaaa aacattcgga
    10861 tgcgttcgtt tctattggaa ctttctacta gatcaacgaa taacgaatta caagaagaaa
    10921 aaagaaaatg ccgattacaa agaagataag acaacgtatg cttcattgaa aaaaatggaa
    10981 gaatacgctt ggctaaaaga agttgaagct caaccactta gccaagtagc catggatcta
    11041 aataaagcat ttaaaaatac gttcaactct acttttggat ttcctaaatt caaaagtaag
    11101 aagtattcca aaaagtccta tcgaacagct atgggtataa aggtgaatag aaactacttt
    11161 tatgtatcca aggttggctg ggtaaaaatg gctgaaacgt tgcgtttcaa tggtaaatta
    11221 atgaatgtga caatcagcca gtcaaaaaca gaaaaatatt ttgcaacttt tttagtcgat
    11281 attgaaaact tccaaaaaga tcctgtgacg gaatccattg gtttagattt gggtcttact
    11341 catttctgca taacttccgc aggtgagaag ataggaaacg agagattcta tcgatcatta
    11401 gaaaaacgct tagtggtaga gcaacgtaaa ctttcaagac gcttagaagt agcaaagaag
    11461 cataatcgta aattagaaga gtgcaaaaac taccaaaagc aaagagtgaa agtggcacgt
    11521 atccatgaaa agatttttaa ccaacgaaat gactttttac acaaactgtc ttctcgtata
    11581 actgatgaga accaaatcat ttgtattgag gacttgaatg taacaggcat ggttagaaat
    11641 cataaattag ctaaatcaat agcagacgta tcttggagtg aatttgtaag gcaattaaca
    11701 tacaagtgtg aatggaaagg tcgaacgtta gtaaaaatcg accgttttta ccctagctcg
    11761 caaatatgtt cttcttgtgg tcataatgac ggtaagaaag agttagatat tagagaatgg
    11821 acttgtagca attgcggaac aaatcatgac agagatatca acgctagtat aaacattaga
    11881 accgaaggac tttcggggat agcttagtaa atttgcgccc gtttgggtgt attacctaag
    11941 aaccccatga ctttagtcgt gtggagtgtc agggtggatt agttgatacg gataaagacg
    12001 gtgtcaaaga agcagtatgg gtagacaatg tagcaaatgc agcgatccta aaatcagata
    12061 aagaagggcg ctttgaaatc actggtttaa ctgaaggtga atattcattg gaagaaacaa
    12121 aagcaccaac tggttaccaa aaattaacag aagatattgc atttaaagta aacaaaaact
    12181 catttaaaga ggaaaatcga atcactgtta aaaataatca aaaagcagct attccattaa
    12241 caggtagtaa tggtttccaa acgtatgtat tgatctcttg cttgttgcta ggagcagggg
    12301 cactttctgc agttgtttac tttaagaaga aagcctagaa ggatcagcta atgattattc
    12361 gtaagccccc aaaaagaaga gtcatgcaca aactgatgat atttttttgg ttgctgtcgg
    12421 cagtagcagt ctatttaatt ccagttgtgg aaacaaatgt agctaaattt caacaaaagc
    12481 aaagaatttt agccattgaa aatcttgaaa aaacagattc ttctttggat gttgcgaaaa
    12541 tagagttagg cgatcctgtt ggtgttctta caattccaag tatttcttta aaactaccaa
    12601 tttatgacgg aacttccgaa aaaattttag agaatggtgt agggattacg gaaggaactg
    12661 gcgatattac aggaggaaac ggtaaaaatc ctttaatcgc agggcacagt ggactttata
    12721 aagataatct tttcgatgat ttgccttcag taaaaaaagg gcaaaaattc tttattaagg
    12781 taaatgaaga acaacatgcc tatcaaattt atcgaattga agaagttaaa gcagaagaat
    12841 tacaaaagaa ttatttagat tatctcatgc ctgaaaaaga ccaagatagg attacgttaa
    12901 tgacctgcac gccaaaaggt gtcaactcac accgcttttt agtgtatggt gaacgagttt
    12961 cattcagtaa agaagattta aaactacaga aaaaagcaaa aaataggaaa tcaatttcat
    13021 tggattggcg attagcactg ctattcgctt tttttgcgtt cctattattt ttatttataa
    13081 aaaaaataag gaggagatcg gaatgaataa gagaaaatgg ctgtttgttt gtgtagcagc
    13141 tctctttgca actgcagctt actcacagat atcttttgct gatagcagta ccactccaac
    13201 taccagtcag acaacagata gttcaacaac tgcaacaagc gacacggaca gttcgaagaa
    13261 cactgcagaa gaaacggcag attcaagtac gagttctact gaatcagcgg aatcaacgtc
    13321 agaaagttct tcagagactt cagaaaaact gcaagaagta gaagatacgc aagtaatcaa
    13381 gcaaaacatt catattcaaa aaattatttc ttcgaatcga atcaacaatc aaggaaggca
    13441 agtagctcat caagaaggtg tcaatggcgc aaagtttatt gtctacgata tcacgaatat
    13501 cttagaagaa atgacacaag atgataatga gaaaaatcaa tcggtagaaa taattgagtc
    13561 aaaattgaaa gatcgagcaa aaaaactttc atacgaccag ttgaaaaaag tgactgaagg
    13621 agaaaccaaa acaattgatg atcaggaagg tgtgattgat ttttcaattg aagtacctgc
    13681 aaacaaaaaa caagcatact atatcgtcaa tgaatcaagc cctgaaaaca tttcaaatag
    13741 cgaagatatt attttattga cacctatttc agatgaaaag ggtgaatttt tgacggatgt
    13801 ttggttatat cctaaatcaa agaaatcaga gccgaaagaa gaaattaaaa aagttgtatc
    13861 aactggtgta aagaaaaact tttttgataa ttgttgggat tttttcactc atttattttt
    13921 taacgattag gatgaaatga tgaaaataat aaaaatgtta gcttttaatc tatctttttt
    13981 agccgcaaca atattgtgtt gcggtttagt tttttctcaa ttatcaattt atccaagata
    14041 tcaacgattg ttcgttcatt ttctaaatat ccagcatgct tccatgccac tagaagtgat
    14101 taacatgctc ttaatcaatc caatttttat ggctagtttg tttcttcttg caggattggt
    14161 aacgtgggta ttaggaaaaa aagaatgaga agcttacaat cgtttaacaa aataatagat
    14221 ggagggcatt atggaaaata aaattcattg cactacggga agagtattta ctttagtgtt
    14281 gtttttgttc cttcttcttt ttgcaagggg aactgcagtc caggccttag aaaaagttga
    14341 tccgagtcaa atcagagaaa ataataatct ggtactttat catgatagtg attcattgag
    14401 aaatatcgaa gtaactggga tcaatggatt taagatacta gatgagtatt caactaaaaa
    14461 acaaaaagta cttgcttggc caaaagtcaa agatttgaaa agcacgtcaa aaattgttgc
    14521 ccattatcaa aaggtgggca cttatctagg aaaagaaatt gattgcgacg taactttttc
    14581 taattttgta gcgcacgctg gggttaacca agacggatta gctaaaacaa ttaatgggaa
    14641 aaagtattat aattatttgc tctttgataa aaacatttat cgaggctttt tcatgcagtc
    14701 aatcaacgtt atgaatgtaa aactagattt taagtattca ggatcaaaaa caaaagtagc
    14761 tttgagtgaa ccacagccag aaattaattc aaaaatgaga tcgatgagag cagctggtat
    14821 tgagttgaat gatacatttc tatctattta cagtttaaat ggacagtatc agcaacgtgt
    14881 gtcttggtat aatgacaata atgaatctgt cggtgaacgt aatattgata tgagagaatt
    14941 tgttgggtat gaaaaaatgg gtagcttacc ttattttgtc acaacagata cagcatgtgc
    15001 agaatacaaa gctcctgcgt tatcaacaaa caatttaact tcaaaagtag tgggaggacg
    15061 tgcagaaaag gcttatagct cgaatgatca tttcaccgat gttgtaggag ctgatactta
    15121 tcacagaagt ggtgtaacgt atacgcttca aggcgcttcc ccaacattca tgattggcgc
    15181 aaatacgaat agtatgatgt ttagctttga tactgcattg ctatggacac cacaaccatc
    15241 gaagcctaca aaagaagtgt ttaacaaagc taatactgaa gaggcagcac acaatattga
    15301 caaaaaagtg attccacaag gatcagatgt ttactatcat attcaccaaa agtttgatgc
    15361 attaacagtc aacacaatga acaaatacaa atcatttaaa atcactgata cctttgacag
    15421 caaaaatttt gatatggtat cggatgggaa aaactatgat ggcgcattgt ggtatgttga
    15481 agcgaatggg acttggaaaa aagctgatac aggaggaaaa ttaactgtat caggtaatac
    15541 agtcacttat actgcttctg acagcttttt agataacgct gcaaattacg gtagggatta
    15601 tattttgcag ctccatatga aagcaaaaaa tgatgcaaaa ggaattgcac caaatgtggc
    15661 tactgtaacg tttaacaatg atccgaacaa aggaatacaa aaaacaaata cagtagaaaa
    15721 ctatttctct cttaaaccaa ataaaaaagt ggaacaagat gggaaagatg ttgatggtcg
    15781 aaatgatggt aaaaaaggaa cgcctacagc tcctttaaat gcaggatctg aagtacaata
    15841 tctagtcaca caaaaatggc atacaaaagg tgtagacatt gcgtcagatc attacaagca
    15901 atttagtatt caagatccag tggaagctcg tttgacgtat aaagaaggtt ctgcgcgagt
    15961 gatagatatg agtacaggaa aagatattac ttcagaaggt acactaacct atgatagcaa
    16021 tttaagaacg ctgaaatggg aagcttccgc tgatttctta agtaaaaatc ttttagatgg
    16081 acgagaaatt caactgatat ttacagctaa aactccacta cagtcagaaa aaaatattga
    16141 taaccaagcc gtagcagcag tagagaatgt ttctaataaa acgaatgttg taacaattgg
    16201 tgttgatcct aacttgccac aagtcattgt tcctagaaca ggttctacac atttagtaac
    16261 aattttagtg gttagcttag tattacttgt gttagttact cttagttatg tggctctgaa
    16321 gttcaattaa acaaaaaaac aaggctgaaa atcagccttg tttttctatt tgaaaattgg
    16381 attggcacta ataaatacgt tgtctgaaga aagtgatcca tctgcagaaa cttcataagc
    16441 atgagataac ccaacgatgc tttgattgtt gtaaagtaag tttgcgttat gagcagcgct
    16501 atctttccac ataatcatgg cattcactgc tcccgtatct gaaaggatct cgtcaacgta
    16561 aaggtagaaa ctattgatat tttctccttt agcgccgtag cagcctaaac tttctaagag
    16621 atcttggaaa gtaccgccgc caggtcgata atgactgaag cttacttgta attcttttgc
    16681 acgtatatca gcagcttgtt gaacgatatc attaaattgc attggagctg caccgttttg
    16741 ttgacgataa ttatttaacc atacaagttc tttttgacgc acatagttgt ttaaatcact
    16801 taatgagata tttttgtatt tgaacgtgat cgttcgattt tggtcaacat tattgattac
    16861 tcgttggccg tttttatcta cgctaatgga aggatcgtca tttctacaca agaaataatt
    16921 ttgatcagtc atatttttcc aaggagtaga gaagctatcc ccttcgtata ctgtttttga
    16981 ttcactagag agaacagcgt tggtatcagc atttacatga ttaacagtca cattaaattt
    17041 acgagtgtag ttgaaaatat aattttgact ttcagtcaca ttgtttaagg ttactgaatt
    17101 tgttggttct acccgatcgt ctaaaacata tccttgaata gcaggagcag ttaaagtgac
    17161 actttcaccg tgagtgacgt tgattatctg ttctttcaaa atatcaactc gcccctcatt
    17221 tttgtagttg ttattgcaca acaaattgat ttgatgcact tgattataat tgaaggtgat
    17281 tgttgtatta cctgtgatcg tttggctttg agtatcatta ccaatcactt tgtagctaaa
    17341 attatcttta gcgccttcgc tatatttaaa tgcttttgcc ttagcggtgt aagtagaacc
    17401 agaagtcact tgtgcttgat ctgtagcaat cacttgacct gtttgattgt caatatggtt
    17461 aatggtaacc atatatggcg tttcaggttt gtgttgcaac cctttaaatg cagcatctag
    17521 actattttta gcattattta cttgggcttg agaagcctta gaatcgtcta gtactttttt
    17581 agcattggat aaagcagtct gaaaatcatt ccaagattct tctgtaaaat ctcctttagc
    17641 agttgatttt actcggttgt ataaagcttg taattcagct ttattcacag taggtgctgg
    17701 ttttttcttt aatcccttgt aagcagaatc taaattattt ttagcactat ccacttgttt
    17761 ttgagtcgct tttgagtcat ttaacacttt attagcatta tttaaagcag tttgaaatgc
    17821 gttccaggta ttgtcggtat agtcaccttt tttcgttccc ttaacgctcg tataaagagc
    17881 ttctaaagct gatttattca ctttgggttt agaggtgtca gcgccatacc aaccgatacc
    17941 ttcgtatttc cagccatagt ctttggttaa ccattgtact tctttccaac ttgcagtgta
    18001 gtgatgtgag ccagatttta caccaggaag gaaaaggcgg tagatcggtt tattattttt
    18061 ttcggcagaa cgccaggcag gcccttcata agtccagccg taatttttag tcaaccattt
    18121 cacttcatcc caactagctg tgtaatggtg atcacgaaga cctttattgt ataaacgata
    18181 gacaagatga ccaccgctag tcggtgcttc ccaagcgggg ccttcataat tcccccactt
    18241 aatcttcact aggtgatcac gttcttttgt gctagaggtg taaaaatgtt catggttatt
    18301 tggattataa aggcgataca tgacgttagt tgaagcagca tcagcagatc cagcgaaacc
    18361 aagtgtggca gcaaatgttc caatagctaa aacagttttt gggtttaata atttcatatt
    18421 ttcaactcct aaaaattagt taaaatcgat tacaaaatca ttatatatcg gtaaaccgat
    18481 atggtcaata aaaaaattaa gaaaagcctc atgtatttct ggcttttctt aatttgttac
    18541 ttagttgaag cgttagtatc aatggtttct tcagctaatt tatttaattt tatagcattg
    18601 atgaatctca tattgtctat actagagcgc ttatttgcga gatcactgac cgtagttaaa
    18661 ggtacgccag attctataga tatagaacga actgatttac cactatcaaa cagcaaccat
    18721 tcgattttct ttttatcagc gtacattgca atgctccctc ttgatttttt ctttattata
    18781 gctctttttt gtaataaatc acaatatcaa gagagaaagt ttttatagaa aggtgggaaa
    18841 ttatgagtat ccgttccccg tgtatcaaaa tgaggtttgt tgctactttc gttatttgat
    18901 atactaaaat aaaacaacga aagaagaaat aaaatgaaga aaaagcaact atggtttgtc
    18961 tctatgtttg caatattttt
//
LOCUS       DACEUZ010000043        18912 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.043, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000043 DACEUZ010000000
VERSION     DACEUZ010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18912
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.043"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(<1..879)
                     /locus_tag="IXG44_001306"
     CDS             complement(<1..879)
                     /locus_tag="IXG44_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HAQ1658036.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQ"
     gene            complement(872..1615)
                     /locus_tag="IXG44_001307"
     CDS             complement(872..1615)
                     /locus_tag="IXG44_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1658037.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(1791..2567)
                     /locus_tag="IXG44_001308"
     CDS             complement(1791..2567)
                     /locus_tag="IXG44_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1658038.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(2815..3651)
                     /locus_tag="IXG44_001309"
     CDS             complement(2815..3651)
                     /locus_tag="IXG44_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HAQ1658039.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(3662..4591)
                     /locus_tag="IXG44_001310"
     CDS             complement(3662..4591)
                     /locus_tag="IXG44_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAQ1658040.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            4783..5136
                     /locus_tag="IXG44_001311"
     CDS             4783..5136
                     /locus_tag="IXG44_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAQ1658041.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(5210..5866)
                     /locus_tag="IXG44_001312"
     CDS             complement(5210..5866)
                     /locus_tag="IXG44_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAQ1658042.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            6092..8026
                     /locus_tag="IXG44_001313"
     CDS             6092..8026
                     /locus_tag="IXG44_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ1658043.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(8112..9335)
                     /locus_tag="IXG44_001314"
     CDS             complement(8112..9335)
                     /locus_tag="IXG44_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronyl hydrolase"
                     /protein_id="HAQ1658044.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(9329..11227)
                     /locus_tag="IXG44_001315"
     CDS             complement(9329..11227)
                     /locus_tag="IXG44_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HAQ1658045.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(11221..12339)
                     /locus_tag="IXG44_001316"
     CDS             complement(11221..12339)
                     /locus_tag="IXG44_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAQ1658046.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(12422..13255)
                     /locus_tag="IXG44_001317"
     CDS             complement(12422..13255)
                     /locus_tag="IXG44_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1658047.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(13248..14165)
                     /locus_tag="IXG44_001318"
     CDS             complement(13248..14165)
                     /locus_tag="IXG44_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1658048.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(14392..15516)
                     /locus_tag="IXG44_001319"
     CDS             complement(14392..15516)
                     /locus_tag="IXG44_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ1658049.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            15699..16607
                     /locus_tag="IXG44_001320"
     CDS             15699..16607
                     /locus_tag="IXG44_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1658050.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            16748..18361
                     /locus_tag="IXG44_001321"
     CDS             16748..18361
                     /locus_tag="IXG44_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HAQ1658051.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT         5973 a         3685 c         3104 g         6150 t
ORIGIN      
        1 ctgatgcaca ttttggttct ttttatgggt tctttttatt ttgaactcac tgtttgaatc
       61 agtcagttgc tctgtaagct ttggtactat cagctgtttt acagcaagga aaaatcccag
      121 agccgtgata gctagtattc cccaacccaa taaaccttgt tgagaaagtg gctgactggc
      181 agcatcatag aatggcaata atatcgaaat cggatgccga tctgttaact cattttcaat
      241 agtccccgtt tgatgattca tccacatgat tccagcaaat gcgatcagca tcgagctgcc
      301 aagcaagata ctagaaactg tttttctatg ttttttaaac actgctgttc gagccaaacc
      361 aaaaacaatc aaactagcca gacaaaaaag aataatcaaa aatagagaaa acaaaaggat
      421 tgagataatg atagtaagca aaatccctag atcagctcgc catccggtta aaataaacat
      481 gactagaagc ggaaaaacaa aaggaatgat ggtcaaagca actaccaaaa tctttgctga
      541 aaaaacctgt tcttgtccaa aaggcagagg caaaaatact tgcaggtcat ttccttcaaa
      601 aaagatatta taaatgactg atatactttg agttaaacct aacataccaa aaagtgccac
      661 gtaataagta aagaaacctg gcaatttaac aaagttcata gcaaacatag caaatccgta
      721 aatcagtaaa aaaatcagcc ctgaaaaaag attttgagac aaaagataga tagttaatcg
      781 ctttcccttt tttcctttct ctcttgcttt attcgtgagc tgtggattaa catataaaag
      841 attaactcga attaattcta ataattgtcg cttatacata cggtccatct cctgtcgctt
      901 gccttccagc cattttcaaa taaatctttt ctagcgtttc atcaggtgag ccattcaata
      961 attcactaac tgccccctga taaatcaatt cacctttctt caaaatagcc agtttatcgc
     1021 ataactgttg tgcggtatcc aatacatgtg tagaaaaaat gactgttttt ccttttgcgg
     1081 catgagcctt catcatttct ttcaaatcaa atgctgcttg aggatcaagt ccttgcaaag
     1141 gttcatccaa tacccaaata tctggatctg gcagcaacgt gccgatcaaa atcgtttttt
     1201 gacgcattcc atgtgaaaaa cttgcgattg tttcattttg atgactatac atatcaaaaa
     1261 gcgctgtcaa ttctgctaaa cgcttttctt ttttttgtgt gtctaattca taggcagctg
     1321 aaatcaagtc ccaatattct ccagccgtca attgtaaaaa aatatctggg gaatcaggca
     1381 cataaccgat tttttgtttg atactcaatc gattttccgt caatttcatt ccatctacta
     1441 aaatcgttcc cgatgatggt tcaataatac taactaaact tttaatcgta gttgattttc
     1501 cggcaccatt atgtcctaaa aatccaaaaa tttccccttg tcttatcgag agagacaagt
     1561 cttttaatgc ttgttttttc ccgtaacttt tacttacatt gatgaattca atcatttctg
     1621 tactcctttt gtttgttatt actttttatt gtacccttcg ttattggtgg agtaaatgtc
     1681 aaaaagagtg cataacaaaa aaggatagaa acgactaggc tgctacttgg gttgttctct
     1741 tctacaggaa aacactcttc taacaggtaa gatatgagtc tttgttttct ttaaaatgat
     1801 tcattttcta tcttttcttg cataaaattc tttagtttat caatacttct ctttcccatc
     1861 tctagttgtt ctccatttgc aaatgttact atttcgttta ctctgctaat agatgtaatg
     1921 ttttcagtat taatgatata agcttttaaa tctttacata aatactcata ttcgagtgat
     1981 tttttatatt ttttaattgt gccgtttacg agaatctctt catctttcaa tactaggcct
     2041 attttatttt tataacctgg catgctcatt agataaagga tttcatcgta atggatatag
     2101 tgatcgctat taccataacg gacagttgtc cacctttttt tcgtagcatg ttgaacagga
     2161 tagttttctt tttctttcaa taagtaactt tggatgcttt ttttattttt ttcataagtg
     2221 aattctttta tcaaataacc actaggcgta atctttcgat taataacagt tattgcttta
     2281 ttcggatgag acgttagata aacaatttta cacttttcat tcttttctct aagcatttcg
     2341 cctagttcga taccgctata ataggcgttc aaatcgatgt ctaggaaaaa aatatctgag
     2401 gaatagattt cattattttt aatgaattca acaaattgta taacgttcaa tccctgaacg
     2461 atctcaaatt tccaaggact taataaaatt ctggattctt ccaaaatttc tactagcatt
     2521 ttttcaaaaa ccaaactatc atccaaaata aatatacgcg ttaccataaa aagtacctcc
     2581 actcgatatt ttactatcaa aatcatttca tacaactttt caacatacat gttaggaata
     2641 aattgtaatt ctcttcacaa tcttttccaa cacctttgaa accgatatca aatactttca
     2701 ggatgctgct tctaaaataa atatcaaaca aatgaataca tcatgcaaaa aaagccagac
     2761 aaccgacgtc cttgcgtgtc agctgtctga gctatgttta tttattttgt tcttttaaaa
     2821 agaacgatac acatactgat ttttcggagc agagactttc tcagcgtttg taacaactac
     2881 agatgggatt tcacgcttga atggttgata atagtctgat tggatggttc cttctatttt
     2941 ataccattca tcatttttaa ccgttactcc ttccggcata tgcgtcaaca aaccaaagac
     3001 tcctgaatct gccacacaat ggataatgcc gaatcggaac aagaagatat ctgtattgct
     3061 atcgttcggt gtttggtaaa caaatccttc atagctgatt tttttgccaa tgaattcgct
     3121 agggtaatta taaattagct ccataacttc taaatagttt tcatctgtga tcttgatagt
     3181 ttgcttatcc ccatactttt ctttcaactc attcatttgg tctaagtagt ctgatttgtt
     3241 gaaataaata ctggtatccg gctttaagta ttgtgtctgc atctccggat cccctactga
     3301 ttctttactt actgggaaat taaatccttt ggcttctacg atcgtagtgt cgagactaac
     3361 tgttggaaac aacgtcccca cgattactgg caaagctagc agtacataag cgattccacg
     3421 ttgataaggt tttgacaacc catggtcatg atcgtgctcg tgtgcctttt cttctgcttt
     3481 tccgtttttg acccacaaat acagctgaac taaagccaaa agagcagata aaatcattga
     3541 aagaatagct aagtagcgat aatggacatt gatatattga tcgagtttgc cagaaacttg
     3601 caagtacatc atcaattcga aatacccaga taaaatcaaa aaccgcagca tattttccct
     3661 cctaaataaa taatgtgaat ccaataacgc taagggcaat cagtccgatc agcgataaaa
     3721 taaagcgtcc tgtaaaatat cgtttcatca tcagtagatt tttgatatcg accattgggc
     3781 caaagaccaa aaaggcagct acaggtgctg tgccgaacag acttaacaaa gaagatccta
     3841 taaacgcatc tgcttctgag caaagagaca tcgtaaaggc caataacagc atgatgataa
     3901 tggctaaaat ctttgttgag ccgagcgtta gcataattct agttggtaaa taagtctgca
     3961 tcgcagctgc gattaatccg ccaatgatca aataacgacc tgtatcaaaa aattcgtcga
     4021 ttgaatgtgt caacacatgc ccagtttgat ggatcagctg attttttggt tcattcttct
     4081 cactttgttc ttcttcatgg acatgatgat ggtgattttc tgctacttta gacgtgattt
     4141 tttctttcaa tacagcgtct ttttgaaaat aggcaagcca gacaccaatc acaagggcaa
     4201 caaaaaagct gcctatcacc cgccagcttg ccatctgcca agaattgcca aatgcgataa
     4261 aagtggaaaa aatcacgatt ggattgatga tcggagcagt cagcataaaa gcaaacgctg
     4321 tgtgaactgg tacgcctttt ttaacaaatt gatgaacgat cggtacgatg ccgcattcac
     4381 atgaaggaaa gaaaaatcct aagatcgaac cagtaaaaat agagaagata cgattttcag
     4441 gaagccatct cttcacacgt tctggggtca aaaatacttg taaggcgcct gagataagac
     4501 agcctaataa tacgaaaggt aatgcttcaa taacgatcga taagaaaatc gtactcatct
     4561 gcaaaaccga atttggaata aaagaaaaca tgagtttcct cctttttatt tctagttcat
     4621 tcgctttgca aatatacgct tttattcaaa aaaaacaagt tgctctgtaa atttttacac
     4681 aactcttatt ttttaccaaa gcacttcaat tgactttttc tgttattccc attaaattaa
     4741 ggtctcatac atatcacttc tatcataaaa gggagaataa aaatgaaaca aaccactaac
     4801 ttgactgaag ttcaagacat actgcaacag tcatccgttg ccgtcattta tctttcgatg
     4861 cctaattgca gtgtttgtca cgctgttcgt ccacgactag aggaacttct cacacattat
     4921 gatattccgg cacttcattt ggatgcattt gaaacaccag aagtagctag taaatttgaa
     4981 gtgctgactg cgccagttgt cctgattttt catcaaggaa aagaagtctt tcggcaagca
     5041 cggttcatcg actttaaaaa aatccgttat ctactagatg aactgacagc agaagatgat
     5101 tccctttctt atgaagaaat cttccgaaca aaataatcgt tgtccaaaca gaaaaaagcg
     5161 attgaacaag tgaaagtttt gccttcattt gttcaatcgc tttttatttt tattctgctg
     5221 tattttcgga atgcgcattt gcgtcagaat gcaagaaata gatcaaggtt tgcaattcat
     5281 tcgtcaaatc aacattttgg atcaatacat ctgacggagc attcaatcga gcagcggtaa
     5341 agttcaagat tcctttgatt cctgcttccg ttaactgatt gactacttct tgtgctcttt
     5401 cagcgggtat cgttaaaatt gctacttcga tttgttggat cttgatttgt tctgacatat
     5461 cttccattgg ataaacgggg ataccgtcta cgatgcgccc aacgatttcc tcattcacat
     5521 caaaagcaca actgatacgt atgctattac tttgatggaa tttgtatttt agtaaagcac
     5581 ttcccaagtt ccctacaccg atcagcgcaa catttgtcaa ttgatcgtcg tttaatgtct
     5641 ttgcaaaaaa gtgcataaga tcttctacat cataaccata cccacgtttt cctaattcgc
     5701 caaaataaga aaaatctcga cggatcgttg cactatctac ttgaacggct tcacttaatt
     5761 cagttgaaga cacttttttc tttccagcat tatttaaaat ccttaaatag cgataataca
     5821 aaggcagtct tttagctgtc gcctttggaa taatatgatc tttcacgaaa tgacctccac
     5881 gtattgtcta attacatctt tgatttctcc atcatagcag tatttatagg aaaaaatcaa
     5941 ttccattgct cagatattgt gaaattttat tctttattca tttatttatc atttctgcaa
     6001 atctcgcagt ataatggaat ttatggtaga ctactaattg aaaaatagtt ttttatttga
     6061 ggagaattca caaagtagaa aagaggattc catgatatta ttacaagcca atcagattgc
     6121 tcgccatttt ggtgcagaag tcttatttga aaacatacat ttagaaatca gtacaggcgc
     6181 tcgtgtcgct ttagttggca gaaatggcgc tggaaaatcg actttattaa agatcattgc
     6241 tggaattgaa gcacctgatg aaggaaagat cgctaaaaat aaaacagcga ctctcggata
     6301 cctcgctcag gacacaggac ttgattccaa tgaaacagtt tggaacgaga tgctgaaagc
     6361 ttttgaacaa gttagacaaa tggaagagcg gatgcgcgag ctagagattt tcattagcga
     6421 aggtacgcca gacacacctg cttatagttc ttatttaaaa gaatacgacc ggctgcaaca
     6481 cgatttttca gatcgaaacg gctatggtta tgaaaatgaa attcgttccg tattgcatgg
     6541 gtttaaattc gattcttcat tttatgatca gaaaatcgat actttatcag gcggacagcg
     6601 cacacgttta gctttagctc gcatgctttt gcaacaccct gacattttga ttctagatga
     6661 accaacgaac catttagata tcgagacact ttcctggctt gagaattatc tgcaaagcta
     6721 tactggtgca ttattgattg tttcccatga ccgttatttt ttagataaag tagtcaatga
     6781 agtctatgaa ttaagcagaa gaaaaatgcg ccattacaaa ggtaactatt cgaaatatct
     6841 agatttgaaa gcagaacagt tagcaagtga atggaaggct tatgaaaagc agcaaacaga
     6901 aatccataaa ttagaagact tcgttgctcg gaaccttgtt cgcgcctcta ccacaaaacg
     6961 agcgcaaagc cgcagaaaga cattagaaaa aatggaccgg ctggatcgtc ctcaagggga
     7021 tgaaaaacaa gcaaactttc tattcagtat agaaaagcct tctggaaatg tagtcttgca
     7081 aatcgaagat gccgctatcg gatatgataa tgtcacctta tctgaaccaa tcaatctaga
     7141 tatccgtcgt caagaagcga ttgctctagt aggtccgaat ggtatcggga aatcgacgtt
     7201 gctgaaatca atcattcacc agatcccttt tatccgtgga aaagaaacgc tagggacaaa
     7261 tgtcaccatc ggctattacg atcaaggtca agctgatctg catacaaaca agacgatcct
     7321 agcagaattg tgggatgagc atcctaccac tccagaaaaa gagatccgaa gtgctttagg
     7381 tgctttctta ttttctggcg aagatgtaga aaaaccaatc cctctgctaa gtggcggtga
     7441 aaaagcacgg gttgctttag ccaagttagc gatgaacaaa gaaaattttt tgattttgga
     7501 tgaaccaaca aaccatttag atattgacaa caaagaagta ttagaaaatg cattgatcga
     7561 ttatgacggc acactgcttt ttgtttccca tgaccgatat ttcatcaatc ggatcgcgac
     7621 aaaagtcgtc gaactctctg aaaatggcag caaagtctat ctaggagact atgattatta
     7681 tttagagaaa aagcaggaag aagaagaaat cgccgaattg ttggcacaag atgagccaga
     7741 atctgctcct gctcccacga acaaatctga ttattaccaa agcaaagaac agcaaaagct
     7801 tatccgtagc cttcaacgaa aaattactca ggttgaagaa gaaatggcgc ggatcgatga
     7861 atggatcgat cagttggaac aagaaatggt cgcgccagaa aacctggaag atcacgtaaa
     7921 actcaatgag ctgaaccaag atttagaagc tgctagacaa gatcaagaaa caaaattgac
     7981 ggaatgggaa gaactaagct tggaattaga agaatatgaa aattaacgaa aaaaccaaaa
     8041 cagaaaccat tcatgctgtt ttggtttttc tttttttatt ttatcaaaca aaaattccat
     8101 atttaccttt ttcaccaata agaagaccaa ttactagtca ttctcactaa agcttctaaa
     8161 taatagtaat caccccaaat acaaggtgta tttaccccat tatttttggg cttattataa
     8221 acagactgtg ttaaaaagcc atcttcttct tccgttgtag catatctttc tttcaattgt
     8281 cgcataattt tccaaacagc agactggtat aagttctttt ctgttttgtc taatagacct
     8341 gattctatta attctaataa tccacaaacc atgatcacca tactagatgt atctttttct
     8401 tctctgctag aatcatcaaa gatcaaatcc caataacaaa tggtatcttc tggcaaccga
     8461 ttcataaaat aattggctaa tattttgctt aaatcaaaat atttttgttc tttcaaataa
     8521 cgataactta atgcaaaacc gtaaatcccc catgcttggc ctctagccca acaggaatta
     8581 tcactataac cttgatgcgt acttccatat cttggttttc catctttgac atccatataa
     8641 aatgtatgat aagttgaacc atcttctctt actaaataat ctgctgcatt ttgaatatga
     8701 gcctgagcag cttttttata tttttgattt ccagtttctt cactcgccca atacaataat
     8761 ggaagattca tgcaacaatc catgatcatt ctaccttgtt gtgaaggatc actaggatct
     8821 ccccaagctt gtataactcc tgccttctcc caaaaacgct gaagtaatgt ttctgcagca
     8881 tcaattgaag cttccttcat ttttcgattt cccgttattt tataatctgc tacggttgat
     8941 aaagaataca aaaaaccaag atcgtgatga ttagtgataa tttgacagtc aatcctgtgg
     9001 ataaaatcta tatctgcttt ttcagctgtt tgttgaaata aaggatcacc cgtcttctca
     9061 aataacaacc aattaattcc tgtccaaaat ccttctgtcc aaccaatatt gtctcttaat
     9121 ggataacgtc cttttatagg acactcgctt gggaattgat actgactgtt actgatacgt
     9181 ttgattaacc tctccgtgtg tctgcgacaa cgctcgatct cattattcca ttctttttcc
     9241 attaattctt tataacattc atttatccat ttttctggat gtattatctc ttcaaacatc
     9301 actttatcaa gcatttcccc accagccttt agcataattt tagtcttttt ttattgtttt
     9361 tattttggat agttagcccg gttgtcccat caaaatcaat tttgggacga ttgttccata
     9421 cttctaaact ataatcccga tcttgatgag caaccactcc tgttccgagc caatgggttc
     9481 cttttccaaa tgtaccctct aatccaggaa taaaagtgtt ttcccatgtc atcaagtttg
     9541 tattgggcaa acccataata gctgtggatt ttcgtttaat agtatcgtca cttaaacaga
     9601 taatacctga aaagccagct tcactcaccc tatagttttc cttgtcttct tctatgatta
     9661 ttccttcttt ctcaggagaa cattcaatag caaaccctgt ctcatataga acatattctg
     9721 tatctaattc tactttgtgt acgcgaatct gccagccttc aaaaggaata agccaagttc
     9781 tgatttttgc ctgtaaagta ggttcccatg tagaggcaag tacctctttc ttacatacaa
     9841 tgtctcgtac attatttcga acgatgaact gtccataggt tttgatgctc tcattcttaa
     9901 aactggtcgt gatatatgtg tccgctcgct gaatccctag cattgaatcc attgccattt
     9961 ctgctttcaa ttgattcgct cgcggtatcg aaaaaccaaa ttgactagaa taagcaaatt
    10021 ttaaatattt ttcattggtc aatgtatgga aatttggccc taattgtccc gcattcaaaa
    10081 aaaatgtatg tccattatca tgttgtatga gcaaattggc ttctgaaaga agtttagttc
    10141 cttcacgaat aggcataggc atttctttag ccgtccaata ctcatggtct tcaggtagcg
    10201 ccaacaataa aaaaatctta ttcagccata atggagataa cgtactatta tagggttcag
    10261 tcaatgtcaa ttgagggtag gtataaccaa tattcaggat acctttttca tcaaatattt
    10321 ctttttgcat ccaccatctt atattccgat tgactaaccc cttgatttca cctaatttaa
    10381 aaggcaaaag atcggcccac aacatagctg accaaaagct tgaaactgca aaacgataaa
    10441 tcatgcttcg accaaaaggc acatttgcac catcttccgc aaaaaaatga atatagtctc
    10501 tagaaaaatc aatagctcgg ttcttatata tctggctaat gggcttttct ggttctagta
    10561 cgctataaat caaaccataa taatgaaatg caaaaggatt ataataatct tggcgacctc
    10621 ttgaactgtc tcgataccag ccattttgaa gataacaatt ttctatatta tcaaatgctt
    10681 ctttcagtgc tgattcttta actggataac caaatttttg gataattgtt ccgacaatta
    10741 tcttaaaaaa ttgccaatta ccatctgcac attcatattc aaaaatttgc gagaaccaat
    10801 gaataatctg gcttttttct cctgtatcta attttttcca taataattgt tcatgatgat
    10861 ataacgctaa taacattgcg ggcatttcta ccatacgctg gtctctttca tgtatttctc
    10921 cccaataatc gggatgggaa ggatctgttc cagcttttat tcctgataga attaatttca
    10981 accatcgttc atctaattgg tcaattgcgg gccccgctcc ccataaaacc cgagagaatc
    11041 cttccattcc tgcaatttta tttccatatc ctaccccttg atgtatataa tcaatttgtg
    11101 cgccacttgc tcgataaaac ggctggagtg gttgatttat ctttttaaat aagtcaaagt
    11161 aatcctgtct ggtccttaat agtggtactg gatgtttcat tgtattttct cctcttttta
    11221 ctaccaaata gctaattccg actgttctaa tcgaagtaag ccttcaataa aaaaataatc
    11281 agaatagatc agtggcacat tataagtcag ttctctatgg tatgcttctg ctccgcctcc
    11341 aatgatccca tcttcatcca tattccaatt tgcatagtgc ttttctgtct tttggagtat
    11401 attgatagca gcttcatgat acatttttga tcgtacagag gaaacatatc ctgctaattc
    11461 cagaagaccg catgcagcgc atactccagc actggtatca atattcgaat cttttacagc
    11521 aaaatcaatg agaggaatat aaccagtttt ttcgattttc tgaataaaat ttactgctat
    11581 cttttctgct atagctagat agtacggatt ttttgtgtaa cgataactta acgcgaatcc
    11641 atataaagcc catgcatttc ctctgctcca acaagaattc tcattgtatc cttgcccgcc
    11701 aagtaaccga atatatttcc ctgattctgc atcaaattct gcgatatgcc cggttgatcc
    11761 atctttcctc actagatact tagctgttgt atctgcatgc tgccgagcaa ttttagagaa
    11821 cctcggatca ccagtttccc tcgaagccca atacagtaat ggtaaattca tcatactatc
    11881 aataatcatc caaccaggtt tatggtcatt ccatgctact aaatagttcc ctgaaatatt
    11941 atagcgtcca gcaagtaccg tagctgcatg taaccctagt tgtcgactat gtttgttttg
    12001 agactgttga taatgtgcaa tagccgatgg taagaataaa aaacctacat catgatgaac
    12061 gtctgaaaaa tggtacaggg cagattctaa taattgttct tgttgtattg ctgcctcttt
    12121 gaacttttct cgttttgtct tgtgaaagag ctgccataaa ataccactcc aaaaaccatt
    12181 ggtccaccag tcgattcctc tgtcctttat tacgtctgta tattttccgt tgtaagaaat
    12241 atatggaatt ttattcccca ttctgtctaa ttccgcatct atcttttcag aagctacatt
    12301 caaaattttt cttgaccatt ccttcgtctg ttcattcatg gatatcccgc cattctttta
    12361 atttttatga atgtggagtt gctcctagaa cgaactagat ggttaacatt cgttattttt
    12421 tttacttatt tgcagctcca atgtcattct ccttaaattt agactgcgcc cacacataaa
    12481 tcaagaccat tgggatcact gaagttgcta aaattagcat aattgcgcca taattaattg
    12541 gctcacctgt actagaaacc gtcatatttt tgataattaa gggaaccgta tatttatctt
    12601 tatctgaaag catgactaat ggaaatagat attgattcca acttcccata aagataagta
    12661 aaccactagt aataatcgta ggtgataaaa tcggtacagc gattttcgta aaaatcaccc
    12721 actcattggc accatcgatt ctggcagcct ccatcagtgc tgtaggaaat tgactgctat
    12781 attgtcgcat cataaacact gaagttgtac tggcaatcga agggatgatc acacctgcat
    12841 aagtattcga taatcctaaa cgactcataa tgtaaaataa aggaatcaat gtggtaaaaa
    12901 aaggcaccat cctagatagc ataactgtat tgaacaacag tttattcccc ataaaacgat
    12961 actttgctaa tgcataaccc gccattgtag aactggctgt agctaaaact gtactgatta
    13021 cagcaataac aatcgaattc caaataatca agggataatt atatctcgcc atgatggctt
    13081 gccagttttt taatagcata tccccaaact tgaaattgat tccgccactt aaaatttctt
    13141 gattactttg cgttgaagct acaatcatat ataaatatgg catcaaaaat attaaggcac
    13201 caaacacaat aatgacgtac gtaaaaattc tgctttgtat cttttgttta gtcattttta
    13261 tcacctccga atttaaattg aatagccgat aatataacag atacaaacac catcgtccag
    13321 gcaatagcag aggcttttcc aaaatcaacc atttcaaaac ttgttttata taaattgact
    13381 cctacgacca aagctgaatt ttctggtcca ttggaagtcc caaagaatat atttgggata
    13441 ttgaaattac ccaagccatt gatagtggct aaaactgtgg ccattaaaat gattggcttt
    13501 agcatgggga tcgttaataa aaagaattgc cgaaatttcg atgctccatc tatttcagca
    13561 ctttcgtata tctctttaga aatcccttga agaccagcta aaaataaaat agtgttatat
    13621 cctacactaa gccaaatcat cgtgatgatc attgatattc gtatccaaaa aggatttgtt
    13681 aaccactgta caccgttaat tcctagcgca ttcaaaaatc cattgaataa cccagtcgga
    13741 ttgaaaaaag tcatgaatac ggaagctaca gccaccgttg atgtaatggt tggaatataa
    13801 tagacagttc taaaaaatcc ttttcctttt aactgtgctg aattcaataa aacagcaaag
    13861 taaatagcta atagcatcgt tattggaatt gatcctgcca tgataatcaa aacattcgtg
    13921 taagatttaa taaacaatgg atctcggaag atagctagat aattgtcaaa tccaacaaat
    13981 tcaaatgagt agccaatttt cataaaacta aaagcaaacg agataatgat gggaatcaaa
    14041 atcgtcacaa taaagaaaat aacaaaaggt gtgaccaatc cataagcact ttttttatga
    14101 gacgtatctg ttgctttttt cttgctgttc tttattgtct tgcttggtaa cgaagattgc
    14161 atcatcgtta agtcctccta atatttttta atcaccagtt aattttacgc cgtacttatg
    14221 agcaaaattt tctgcttctt ttagatatga attttcaaaa tcacctgttt gccaatagtt
    14281 atatgcagcg gtagtcaaat aagacgtagc atctgaataa gtggctggta aaacagtatt
    14341 tgatgtattt tcattgattt tattcaatag ttcatagtat ttttgtcctc cttagtagtc
    14401 aaactttttc tcagctgctt ctgtttgata ggccaatgag ttagatgtag ccacgcccag
    14461 ctccagtgcg ttggctaagc attcttcatc tgtcagcata aatgtaagaa attgttgtgc
    14521 cacttgtgga tttttggcat ctttagggac ataaaaactt gatcctcctg atactgcacc
    14581 ttttcctgga ttttcttttg aaaaaggaat cagtggagcc atgcgccatt tgtcagatgc
    14641 atcaggaaaa ttcaaagaca tattcgtagc ataccagccg ccagcaacaa acattgcaga
    14701 ttcttgaaat gctttctcag catcttgtgc accgtagaaa tgtacaatat ctgagtcgat
    14761 catttctttg attaactttg cagcatgttt cgcttcttta gtattcagac ggatatttcc
    14821 ttcttcatca agcaaagatt gattctgctg ggctagcatg ttgataaaca attcttggtc
    14881 cccagattct ggcaaggcaa ttccaaaact accagttttt tcttttaatg cttttgccac
    14941 ctggatatat tcttcccaag aactgatttc ttcaaaatct acacctattt cagcaaattt
    15001 ttttggttga taataacaaa gtgctggggt aaaatcacta gcccaaggaa taatcgtttc
    15061 attactcgtt tgttgacgca aaatattcaa tttttgatca taaaactgat cgccatattt
    15121 gtcataaaat ccatattcat cagcactata aaaagcatct tgaaattttt ccagcacacc
    15181 tgctgctgaa gtgtctgcca tggagataat atccggcata tcttcgtgtg ttaccaattt
    15241 tggggtgatc atttgtactg ttgctgttcc cgaagctaca ttcacaattt ttaagtcaat
    15301 atttgctcca gttttgcggt tatagatttt tgcagcattt tcaacatagt ttgtcagttt
    15361 gccgtttgcg acatatactt ctaatgtagt ggcttttttt atcattggat ctgcgatgtt
    15421 tgctgttact tcttcgttag tttttccaca tgcacttaag ctgaataata gggtaatgtt
    15481 tagcaaagct atcaccaatt tgtatctttt tttcatgtct atcccttctt tcttttcaag
    15541 ttcttatata aaacgctttc acttatgtat attaacggaa ttagtaacac aaatatttat
    15601 ataaaaaaat attttacttg tcgttttggc ttttcatttt tgttataata gtcattaatt
    15661 ataaatagaa agctttttat ataaaaagag aaaggaaagt ggttcttttg ttaacaaaca
    15721 accaatcctc caatcttgtc aaaaaaacac taacctattc catagatacc catcttcatt
    15781 tatctgctgc tccctatttt cgactactaa taaatgaaag cgctaattta tatgctgaat
    15841 ttgcacataa atctatagaa ctagataatc cttccctttt attcattgat aaacagcaag
    15901 aagtccaaat cttttctact caaaaggaaa cacaaacttt tatcattatc cagttttatt
    15961 ttgatgagca attattagca catattacgg gacaatcttt ttctatagat caagaaataa
    16021 catctatcat tacttttctg aaaaatgaac agttcaaaat tgattatcta aaacatagta
    16081 agccagctat ttcctatgat caggaaatca attctttttt catactatgc caacagatga
    16141 gcattcattt gctttcctta ttagttactt attgtctgca aaaaattcaa ttgtcttcct
    16201 taccaaggca ttatcaacag gaacttgctt tattgacaat accttcaaat gagaaagtca
    16261 ttcctaaaaa aaagcatatt ctttattcag atgaattagt tgatccaatc atacgatatt
    16321 tagaagaaca tctttcagaa aattttttgc ttgaacaatt agcccaaatt ttttttatca
    16381 gtccagccag cttaaaaaaa cattttagaa aagtgaatgg aaaaagtgtt attagctatt
    16441 ttaaagaact aaaaatggaa aaagcaaaac gtctcgtgct agaaaatgaa ttaacctata
    16501 cagaaattgc agatatatta ggatttcatt cgatccatca tttttcttca gcttttaaaa
    16561 aatatacagg gttatcccct tcaaagtatt atctatcctt aaaatagaaa aaaattttta
    16621 ggttttatcc acaaattatt tctctatatt ttctgtctat tcaaaataaa caataatttt
    16681 ccggcacaat tatttgctgt tttttaccat aacatagtac gatcaacgta gaaaaggaga
    16741 tgtttttatg aaagttgttg tcattggcgg tgtagctggc ggtccttcat ttgccactcg
    16801 tttccgtcga ttgaatgaag cgcacgaaat cattatctat gaacgcggag aaaatatttc
    16861 ttatgcaagt tgtgctttgc cttattattt aggaggtgtg atcactgacc gtgactcgct
    16921 gatcgaacgt acaccagaaa tattgaaaac aaaaaacaac atcgatgtct ttactaaaca
    16981 cgaagtaaca gcaatcgatc cttctactaa gcgattaaca gttaaagacc tatccacaaa
    17041 tgaagaaata aaaacagatt acgataagtt gatcatctct tctggtgcta gaccagatta
    17101 tccggatatt cccggagttt ttgaagcaga aaacggcttt gtcctccgta gtgtgacgga
    17161 tgcggatcga atcaaatcgt tccttgaaga aaaaaatcca caacatgtcg tcattcttgg
    17221 tgcaggtgtt atgggtctgg aattagctga gaatttcaag catcgcggct tagacgtgac
    17281 tttaatcgat caattgccac aagtcgcttt cccttatgat ccagaaattg ctaatttagt
    17341 ttatgacaaa ttgctgaaag aaggattagc cgttcattta gaaacaagag ttactgagat
    17401 ccgtgataaa ggtcgagaaa tagtattatc agatggctcc gtcctttctg ctgatatgct
    17461 aatttttgct gttggtgttt ctccgaagaa tgaagtggtg aaagcaaccg gcatacaatt
    17521 atcagataca ggtcagatca ttgttgatga ccagttacaa accaatctgc cggacatcta
    17581 tgcgattggc gatattatcg aaacgactag tgtagtgact ggtcagccga tccaaagtat
    17641 gctttccagt gcggccaatc gtcaaggaca tatgctggca gatattttaa atggtacgcc
    17701 tatgcgctat cgcggatata ttggtgcagg tgtcgcaaaa atctttgatc atacagcaag
    17761 ttatgcggga atgacagaac atgaattaaa agcatcaggc ataacaaatt ataaaactgt
    17821 ttttatcact ccctttgacc atgcctattt ctatccagaa gctacaagat taaatctaaa
    17881 gctgattttt gatgcagata gcggtcgtat tttaggtgga caagcatttg gagaaaaagg
    17941 tgtcgataaa cggatgggag aactttctgt agcaatcacc ggaaacttga cagtctttga
    18001 tttgcctgat ttggagttgc cttactctcc accgtattct actacccgtg acccgttgaa
    18061 tatagctggt tatgtcgcaa tcaatcaaat gacgaatatc gtagaaacga tcaaagcaag
    18121 tgatataccc gaaaacgatt tgaaagaagc gttcttttta gatatacgtg aacctaataa
    18181 agcaccttct ggcagtatct cggcaacaaa aaatattcca atgaatgagt tacgagatcg
    18241 aatcaatgaa atcccaaaag ataaaaaagt ttacattact ttcagaagag gattgaatac
    18301 ttatacttct gcccgaatct tggcaggttt gggtatcaaa gcgattttga ttgaagaata
    18361 aaaaaacgac cgcggtgggt agccgcggtc taggagttat ttttacttga ggagtaaaaa
    18421 tgaaaaagta tatagggttg ttggttggta tgtatataga atagaagata aatgtgaaga
    18481 aattgtgcat tatttcttca catttattag gttatagttt aagaaagttt aaacattgcg
    18541 caatttaatt attttagctg tttcttttca taaaaaaaca tgttgtgact ctgagacaaa
    18601 tcatagtcca tagtgatgtc tctagagtaa ctacgcctaa tattacgagg catcatatta
    18661 atctatcagc actagtacct aaactagcat ccctttcgaa tattttttct tatataataa
    18721 agtttgcttt cactcctcta atctcaccaa atcgactggc ttgcgttgtg tgatactagg
    18781 aactgattgg tttagaacga cttttctatt gtaatcatat ttttcgccat cctcgttgtc
    18841 ttctaatcct tcaatgaatg atccctaatg tgtgtattaa ataatttaat ttttttgttc
    18901 ctatttattt aa
//
LOCUS       DACEUZ010000044        18504 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.044, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000044 DACEUZ010000000
VERSION     DACEUZ010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.044"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            99..746
                     /locus_tag="IXG44_001322"
     CDS             99..746
                     /locus_tag="IXG44_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAQ1658052.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     gene            1139..3784
                     /locus_tag="IXG44_001323"
     CDS             1139..3784
                     /locus_tag="IXG44_001323"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HAQ1658053.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            4094..5407
                     /locus_tag="IXG44_001324"
     CDS             4094..5407
                     /locus_tag="IXG44_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HAQ1658054.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            5397..6050
                     /locus_tag="IXG44_001325"
     CDS             5397..6050
                     /locus_tag="IXG44_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ1658055.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            6105..6797
                     /locus_tag="IXG44_001326"
     CDS             6105..6797
                     /locus_tag="IXG44_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HAQ1658056.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            6947..7147
                     /locus_tag="IXG44_001327"
     CDS             6947..7147
                     /locus_tag="IXG44_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ1658057.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(7401..9080)
                     /locus_tag="IXG44_001328"
     CDS             complement(7401..9080)
                     /locus_tag="IXG44_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAQ1658058.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(9082..9294)
                     /locus_tag="IXG44_001329"
     CDS             complement(9082..9294)
                     /locus_tag="IXG44_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HAQ1658059.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9687..11417
                     /gene="efrA"
                     /locus_tag="IXG44_001330"
     CDS             9687..11417
                     /gene="efrA"
                     /locus_tag="IXG44_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HAQ1658060.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            11414..13174
                     /gene="efrB"
                     /locus_tag="IXG44_001331"
     CDS             11414..13174
                     /gene="efrB"
                     /locus_tag="IXG44_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HAQ1658061.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            13269..13466
                     /locus_tag="IXG44_001332"
     CDS             13269..13466
                     /locus_tag="IXG44_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1658062.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            13619..14104
                     /locus_tag="IXG44_001333"
     CDS             13619..14104
                     /locus_tag="IXG44_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HAQ1658063.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(14208..14801)
                     /gene="clpP"
                     /locus_tag="IXG44_001334"
     CDS             complement(14208..14801)
                     /gene="clpP"
                     /locus_tag="IXG44_001334"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HAQ1658064.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            14981..15508
                     /locus_tag="IXG44_001335"
     CDS             14981..15508
                     /locus_tag="IXG44_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HAQ1658065.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            15599..16336
                     /locus_tag="IXG44_001336"
     CDS             15599..16336
                     /locus_tag="IXG44_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAQ1658066.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            16340..17257
                     /gene="miaA"
                     /locus_tag="IXG44_001337"
     CDS             16340..17257
                     /gene="miaA"
                     /locus_tag="IXG44_001337"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HAQ1658067.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            17259..18488
                     /gene="hflX"
                     /locus_tag="IXG44_001338"
     CDS             17259..18488
                     /gene="hflX"
                     /locus_tag="IXG44_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HAQ1658068.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT         5984 a         3200 c         4022 g         5298 t
ORIGIN      
        1 atttgtctca cacccttttc cttcctactt gtaaccttgc tcccgtgtta gaaacatgtt
       61 acactaaata tgtgaaatta ttaacaagga gcgtcgttat gcgagaaaaa acagatacac
      121 tgccaaaagc aactgcgaaa agattacctt tatatttacg ctacttgaag atgctggatg
      181 actcaggtat aagtagaatc aaatcaaatg agttcagcga aatcacccaa attccttctg
      241 caacgatccg cagagacttc tcgcaattag gcgagctagg cagaagcgga tatggctatg
      301 atgtcccttt tcttatcgat gtattcaata atatccttaa tactaaagaa gaaaaacgaa
      361 tcgcattagt aggatatgga aatcttggca aagcattgaa acacaacaat tttagaagaa
      421 atgaaaatct aaatatcgtg tgcgtctttg ataatgatcc agcactgatc aatcgagtaa
      481 ttgatggaga aatgatctat ccgattgatc gtttcgcgga gatagccaaa gcaaaaaatg
      541 tgacagtagc aatctctacg gtaccaagta aatattcaca atctgctatt gatgaaattg
      601 tcaaaggaaa tgtaaccgct atacttaatt ttgcgcctga cagagtaact gtcccagctt
      661 atgtcaatgt tcaatacatt gatttgacaa ccgagttgca gacgcttatt tactttgatg
      721 agaattactc agaagttttt tcttaggaaa atttgagtag gcttattgat cttgcgagaa
      781 ctcgtcgtaa ttctttcaaa aattttttag tactaaatga agtggttttt tcacgaccac
      841 ttgttgacta aaaaaaaaaa ccattgtaga atgaagcata acgctgaaca agaggagtaa
      901 ctggcacatt cattcttaga gagagaacta tctgctgaaa ggttctcaat gacaaccagt
      961 gaaggtagct tgggagtttc tgtcttgaat ggagtagaga tagacgagtc gtgatcgtta
     1021 actcacttga gaggcaataa tgattgatta ttgcaattta ggtggtaccg cgtaaatcat
     1081 acgtcctaga atacgaaagt attttaggac gttttttagt ttaaaggagg aaaacagcat
     1141 gacggaagaa aataacctat caacaaaata taatccgcaa gaagttgaag ctggacgtta
     1201 tcagcattgg ttagaacaag atttgtttaa accgagcgga gataaaaaag caaaacctta
     1261 ttcaatcgta atcccgcccc caaatgttac tggaaaactt catttagggc atgcttggga
     1321 tacgaccttg caagatatgc tgattcgtca aaagagaatg caaggatatg atactttatg
     1381 gctgcctggt atggaccatg caggaatcgc gacacaagca aaagtagaag cgaagcttag
     1441 agagcaaggg atttctcgtt atgatctagg tcgtgaaaag ttcatcgatc aagtgtggga
     1501 atggaaagaa gagtatgcga gccacatccg cgaacagtgg gctaaaatgg gcttatcttt
     1561 agattacagt agggaacgtt ttacattaga tgatggatta tctgaagcag tacgtaaagt
     1621 ttttgttacc ttatacgaaa aaggtttgat ttatcgtggt gaatatatca tcaactggga
     1681 tcctcaagca cgaacagctc tatctgatat cgaagttatt cataaagata tcgaaggcgc
     1741 cttttatcat atgagctatg aactagcaga tggatcaggc gtagtagaaa ttgcgacaac
     1801 ccgtccagaa acgatgttgg gagatacggc gattgctgtt catccagaag acgagcgtta
     1861 tcaagcgctc atcggcaaaa aagtgattct acctttagtc aataaacaaa tcccaatcat
     1921 tgcagatgaa tatgtagata tggaattcgg gacaggtgta gtaaaaatca ctccagcaca
     1981 tgatccaaac gactttgagg taggtaatcg tcatgatctt ccacgcgtaa atgttatgga
     2041 tgatgatggt acgatgaatg aactagcagg aaaatatgct ggaatggaac gttttgctgc
     2101 tcgtaaagca atcgtgcaag atctgaaaga aatcggtcga ttgattaaaa tcgatacgat
     2161 gatccacagt gtgggtcatt ccgagcggac aggtgtcgtt gttgaacctc ggctgtctac
     2221 tcaatggttt gtaaaaatgg cgccgttagc gaaaaaagcc attgataacc aagaaacaga
     2281 tcaagcagtg gaattcttcc ctccaagatt caatcaaacg tttttacgtt ggatggaaaa
     2341 tgtccatgat tgggtaatct cacgacaatt atggtgggga catcaaattc ctgcatggta
     2401 tcacaaagaa acaggtgaaa tgtatgtagg gatggaagca ccagcggata gtgaaaactg
     2461 ggtacaagat cctgatgttt tagacacttg gtttagttca gctttgtggc ctttctcaac
     2521 aatgggctgg ccagatacgg aaagcgaaga ttataagcga tacttcccta ctagcacttt
     2581 agtaaccggt tatgacatca ttttcttctg ggtaagcaga atgattttcc aaagcttgga
     2641 atttactggc gaacgtccat ttgagaatgt cttgattcat ggattgattc gtgacgaaca
     2701 agggcggaag atgagtaaat cattaggtaa tggaatcgat ccgatggaag taatcgaaaa
     2761 gtatggagca gatgctctcc gctggttctt atcgaatggt tctgcaccag gacaagatgt
     2821 ccgcttcagc tatgaaaaaa tggatgcttc ttggaacttt atcaacaaaa tctggaatgc
     2881 ttcacgtttc gttatcatga atatcgaagg aatgaccgta gaagagatcg atttatctgg
     2941 agaaaaatca gtggctgacc ggtggatcct tactcgtttg aatgaaacga tcgaacgagt
     3001 aactgagcta tttgatcgtt ttgaatttgg tgaagcaggt cgacaactgt ataactttat
     3061 ctgggatgat ttttgtgact ggtacatcga aatgagtaaa gaagtacttt atggagatga
     3121 tgctgtttca aaacaaacca cccgtagcat cttagtccat acacttgatc agatcctacg
     3181 tttattgcat ccaatcatgc cattcgttac agaagaaata tgggaacata ttcctcatca
     3241 gggaaattct ctagtagtgg cagaatatcc tgttgtacgt ccagagtttg atgatgaaac
     3301 agcttctaaa gggatggaag tattgaaaga attgattcga gccgttcgaa atattcgttc
     3361 agaagtgaat acaccattgt ctaaaccaat tacgttgcta attaaaacaa acgatgctaa
     3421 aatcgatcaa tttttagttg aaaatacaag ctatatcgaa cgcttctgta atcccgaaga
     3481 gttgacgata tcaagtgaga tcactgcgcc agatctagcg atgtcagctg ttctgacagg
     3541 tgcagaaatc ttcttgccgt tagcaggatt gatcaatatt gaagaagaaa tcaaacgatt
     3601 agaaaaagaa ctggaaaagt ggacaagcga agtaaaacgt gtccaaggaa aactttctaa
     3661 tgaacgtttt gtttctaacg cgccggaaga agtagtagag gcagagcgtg caaaagaaaa
     3721 agattattta gagaagcaaa cggctgtcaa agaacgaatc gaaagtttgc gtactattca
     3781 ataagaatag aaaaagagag tgtgaaatca gctagctatt actaaaagct agctgattga
     3841 acatcgttac aggaaagagt ttgagcctca ctcttagctt cgattcataa ggagatgtga
     3901 cataaatttt gtcgcatctc ttttttagtc tactcttatt atgtaggaga aggagggagg
     3961 tggatcatgt atcaagagtc aataaagtag ttgataaaat cgttcctctc tctgtttctt
     4021 ttctctttgt catactttta tttttcttat gtgttctatt tgttataata aaagagaaat
     4081 gggggaattg ttgatgacga ttgaagaagc aatagaatgg atccatagtc gattaccatt
     4141 tggttcacgt cctggccttg accgagtcga agctttatta gaagtggtag ggcatccaga
     4201 gagaaaagta aaaacgatcc atatcgctgg aacaaacgga aaaggttcta ctgtagctta
     4261 tttgagaagc ctattagaag aggctgggct gacagtaggt acatttactt caccttatat
     4321 cgaaacgttc aatgaacgga tcgcaatcaa tggcaaaagc attccagaca atgatttgat
     4381 ccagcttgta aaaaaatatc agccactgat caaacaactt gatcaatttg aagcagtagc
     4441 tggtattact gaattcgaga cattaacagc aatggcattt gattattttt tgatgaagaa
     4501 agtggacgta gcgattatcg aagttggctt aggcggctta ttggattcta ccaatgtagc
     4561 tgtaccaatg ctgacgggaa taacaacgat tggacttgat cataccgaaa tacttggaga
     4621 tacaattgaa gaaatcgctg cacaaaaagc tggaatcatc aaacaaggcg ttccagtagt
     4681 aacaggaaat attgccccag aagcttttac tgtcatccaa caaacagcta aagcaaaaaa
     4741 tgcaccgctt tatgtatacg gggaaaatta taaaataaac tatctgcatc ctgatgagat
     4801 gtggggagaa gtatttgaat tttttaatga aataggaaag atccctcaat taaccactcc
     4861 gcttttaggg caacatcaag ctgaaaatgc aggagtagcc atcgaattgt attttgttta
     4921 ctgtcaattg gaaaagctgc ctttccgaga aaaagatgtt ttgaagggat tgaagaaatc
     4981 tcgatggcca ggaagaatgg agcgtttgag taacgagcca ttagttgttt tagatggtgc
     5041 ccataatacg catgcaatga accggcttgt tgagaatttg aaaaaagaat tcaaaggata
     5101 tcaaatcaac attttgtttt ccgccttacg aactaaaaat gttagagaaa tgctagaacg
     5161 attgcagcaa gtaccaaatg tccatattta tttgacgacc tttgaacatc caaaagccat
     5221 cgaattaagt gaagtatcag atttagcaag tgagagagta tcaatcgttt cattgtggca
     5281 attcggatta gcagatatat tggaaaagat gacaaatgaa gacgtattag ttgtgaccgg
     5341 ttcactttat ttcatttcag aagttcgcca tttattaata caattaggag gagatcatga
     5401 agtataatgc agtgattttt gacatggatg gtttgctgtt cgacacagag ttagtgtatt
     5461 acgaagccac acaagtagta gcagacaaga tgggttttcc ttatgataaa gacctgtatt
     5521 taaaattctt aggtgtttca gatgaagaag tatgggcgaa ttatcatatg attttctcgg
     5581 aatttggcaa aacaagtgta caaagattca tagatgattc ataccaggaa acgcttgaac
     5641 gtttttcgca aggagcggta caaatgaagc ccggtgtgtt ggaactcctc tcttttttag
     5701 aagaacatca gattcccaaa gttgtcgctt ccagtaacca acgagcagtc attgaacttt
     5761 tattgaagaa gaacaatctg tttgaacgat ttgagacgat tgtttctgca gaaaatgttc
     5821 aacgagcaaa accagaccca gaaattttct tgttagctaa tcagaagcta ggtacagcga
     5881 agaaagaaac attgatttta gaagattcac aaaatggtat tttagcagct tctgcggctg
     5941 agatcccggt agtcatggta ccagatctgc ttccaccatc ccaagaattg atcaaaaaaa
     6001 cagctggggt tgtttcttct ttacatgaaa tccctgcatt gttaaaataa aaatccatct
     6061 cttttcgtat cttattttag ataagataga aaggagatgt ttttttggga aaacgattga
     6121 tcaaagaagt acctactagc tctttaccaa gggaacgtat ggaaatttat ggcgcggaag
     6181 ctttatcgga tcaggagctt ttggcaatct tactgcgtac cggtcaacac ccttatagtg
     6241 tgatgtcgat tgctggaaat ctgttaaaaa cgtttggagg actggcctct ttacgacagg
     6301 ctactttgca tgagttagaa gagattcgag ggataggacg cgtgaaagca atcgaaatca
     6361 aagccttgat cgaattaggt cgacgaattc agacggatca tgaaagagcg gctccaccgg
     6421 ttcgtaccag ctacgaattg gcccagcaat tgattctgga aatgaaagat cacaaacaag
     6481 aacatttggt ttgtatttat ttggacacga agaatcaagt gatcttaaaa aaaacagtgt
     6541 tcatcggttc gctgaatcag tcagtcgctc atcctagaga aattttccat tatgctgtac
     6601 gttattgtgc agcaaggatc gttttagcgc ataatcatcc ttctggtaat gttattccat
     6661 cgcagcagga tatgaatttt accaaaagga tacaaaagtg tggagaaatg atggggatca
     6721 ctgtactgga ccacttgatc atcggacgca aacgatattt tagtttgaga gaagaaggaa
     6781 tgatggaaga gaagtaagtt gctttcttgc aaaaaaaatg aagcaagtgt acaattatat
     6841 tgtaattttg tttgggaata gaagttgaaa catctgttgc acctctatcg tacacgagca
     6901 atgttacaat ttaaattagt gcttttggca cgaggaggat attattatgg aaaacggaac
     6961 agtaaaatgg ttcaacgctg aaaaaggatt tggatttatc tcacgtgaag atggaagcga
     7021 cgtattcgtt cacttctcag ctatccaagg tgacggattc aaaacattag aagaaggtca
     7081 agcagtgact ttcgacgttg aagaatcaga ccgcggccca caagctgcaa acgttgtaaa
     7141 agcttaattt tatctgtaaa tcaaattgat ggtagacatc aatttttacg agagaacaaa
     7201 gcgagtaaca aaaatacctg ccacaagaga caatttctct tgtggcaggt atttttttgt
     7261 atgaaaaagt aaataaattt aagaggctat gaaagagcca ccttgtatca aggaataaga
     7321 acaaacaaaa atggcttctc atgtttttca tttgttttct cttatattca ggacaaggtg
     7381 ctgatttcat aacctcttat ttattcttca tctggtgtca ttaccattgg caaaatcatt
     7441 ggatgtcttt ctgtacgttc gaataagaaa ggctgaagcg cgccagtcat cgcttctttc
     7501 aatttatatt ctgaacagtc aggtccattc aaacattctc ttaaagcgtg gaataagatt
     7561 cgctgagctt cattgatcat ttctcctgat tctcgcatgt agacaaatcc acgagagaga
     7621 atatctggac cagccaagat ttcttttttc ttgatatcca ctgtcgcgac agctaataca
     7681 agaccttctt ctgaaagcag gtgacggtca cgtaaaacga cattaccaat atcaccgatc
     7741 ccgctaccat cgatatacac atctccagcc acaaaatgcc cagcaggacg cgcagaatcg
     7801 ctagttaacg ctaaaacatc gccattttcc atgatgaagc agttttctgc aggaacacct
     7861 acatcttgtg ctagagaagc atgtactttc aacatgcgga attctccgtg gacaggaata
     7921 aaatattttg gcttcatcaa acgaagcatt aatttttgtt cttcttgtcc accgtgaccg
     7981 gaagtatgga tgttattgat ttttccgtga atcacttctg caccagcttc agacagcaaa
     8041 ttgatcaaat gattgacact tgttgtatta ccaggaatag gtgaacttga aaagatcact
     8101 gtatcgtctg gctggatttg gatttgacgg tgagtaccat tcgcgatgcg gctcaacgcg
     8161 gccatcgctt ctccttgaga acctgtacat aggatcatcg tttcattggc aggcaagcga
     8221 ttgatttcgt gtgcatctac aaatgtgccg tctggaactt tgatatatcc taaacgttga
     8281 ccgttgacga tggcattttc catgcttcgt ccgaaaacag cgattttacg accagttgcc
     8341 acagcggcat ctgctgcttg ctgcaaacgg aagatgttag aagcaaagct ggcaaaaatg
     8401 atacgtcctt cgatttttga aaagatcttc aaaatagaat gcccaatcgt tttttctgat
     8461 ttagtaaatg ttggaatctc agcatttgta ctatcagata aaaggcacaa gactccttct
     8521 tccccgatac gagccatttt atgcaagttt gctggttcgc caacaggagt gaaatcgaat
     8581 ttgaagtctc cagtcgctac gatatttcca gaaggtgttt tcaccacgat ccctaatgca
     8641 tctgggatac tatgggtcgt tctgaaaaag ctgacagaag tctttctaaa acggatgatc
     8701 gtatcttcat tgatctcatg gagctctgca tctcgaagca agccatgctc atctaatttg
     8761 tttgtgatca acgctaatgc gagaggacca gcatagatag gaatatttgc ttgtttcagc
     8821 aagtagggta ttccgccgat gtggtcttcg tgcccgtgag taatcaccaa ggcttttact
     8881 ttgtgtaagt tttgtatgat gtagctgtag tcgggaatga catagtcaat tccaagtaaa
     8941 tcgtcttccg ggaatttgat cccggcatca atcaaaatga tttcatcttg gaattgtacc
     9001 ccatacgtat ttttcccgat ttcacctaat ccaccaatag caaagacgcc tgtctcgtta
     9061 tttttgatgg aaagtttcat attaaaactc cactaatgag aaatcagcat tttctttttc
     9121 gtactctaga tgacttcctt ctagaagttg aacgaattca atattgtatg gggtattttc
     9181 ttccacacgt tggcgagcgt ctacatcgct ttttgcctca atgtaaagag attttgtttc
     9241 ttcccgttta gggttgcgta cttttgtttc ttgataataa actttataga tcattgtttt
     9301 gtctccttta tctgcgtaaa gcagtgattt agctagttga tttttttaga caacacaaaa
     9361 cgaggggaca agccacctgg tttttcatat gctttgacta gaatactgga ttcgagtcaa
     9421 agagacaagc ctcgtttttg cttatctgtt cggatatttt ttcaaccgta ccttctgtat
     9481 aaaaaatata caacaattaa ttgttattct agcataaaac agcaaataag tatagaaatc
     9541 aactagattg aaagaaaaaa tcaatgttta tcatcccgct ataatcataa tgactttttt
     9601 atgagagaag tgttctatta ataggctcgt tgtgctatca tttttagtaa tgaaaatgta
     9661 acaaaagatt aaaaggagat gagaaagtga aactaatgtg gcattatacc atgcgttata
     9721 agaaattctt gtttttcaat ttcatatgcg tatttggttt tattttgatt gaattaggac
     9781 ttcctacgat tttagcacgt atgatcgatg taggaatccg caatgatgat tttgattatg
     9841 tgaagcaaca agggatattt atgatcgtta ttactgtaat cggtattatt ttgaatatct
     9901 tgttaggtta ttttggttcg aggattacga ctaatatcgt agcagatatt cgagatgatt
     9961 tgttcgaaaa gatccaaagt tattcgcatc aggaatacga aaatctaggc gtatcttcat
    10021 tgatcactcg tacaacaaac gatgcttatc agatcatgtt gttcctgcaa aatattttac
    10081 gtatcggttt tatggcacca atgatgttca ttgtcagttt gtacatggtc atgcgtacaa
    10141 gtgtgacact cagtttgtat gtagtaggtg cattaccttt actattattg gcagtcgtag
    10201 cgattgctaa ggtgtctgaa ccgttatcta aaaagcaaca aaaaaactta gataaaatca
    10261 acagtatctt gcgggaaaat ctttcaggat tgcgtgtgat tcgtgcattc gtcaatgaaa
    10321 agtttgaaga aaaacgtttt gctgtagtca atgagaatta tgcttctagt tcaaaaagct
    10381 tattccgttt gatggctgtt gcacaaccgg gtttttattt cttatttaat atcgtaatgg
    10441 tattgatcat ctggagcggg gcagtgcaaa tcgatgcagg cgagcttcgt gtaggagatt
    10501 tgattgcgtt tatcgagtat atattccatg ccttgttctc attcatgtta tttgctagtg
    10561 tgttcatgat gtatccaaga gcagctgttt cagctggacg tatccaagaa gccctagatg
    10621 ccaaaccttc gattgtagaa gatccaaatg gtatatctga tacaaaaact aaagggtata
    10681 tcgaatttca aaatgtaaca tttgcttatc caggccattc gcaagagcca gtgatacgaa
    10741 atgtcagttt tacagcctct ccaggagaga ccgttgcttt tattggaagt accggtagcg
    10801 gaaaatctac gttgatccaa ttgatccctc gcttttatga tgtgagtgaa ggaaagatct
    10861 tgatcgatgg agtagacgtg agagattatc ggctaagcaa gttgcgagat aagattgggt
    10921 ttattccgca aaaagcgctg ctatttactg gaacgattgc agataatttg cgatacggga
    10981 aagaagatgc tacgcaagaa gaaatggaac gtgcagctga gattgctcaa gcttcagaat
    11041 tcatttctcg aaaaccagat ggatttaatg aacatttatc agaaggtggt tccaattttt
    11101 caggtggaca aaaacaacga ttggcaatag ccagagccat catacgccgt ccggaaatct
    11161 atatttttga tgacagcttt tctgcattag attatcaaac ggatgcgaag ttacgtgctc
    11221 gtctgaaaaa agaaacgaca gaatctactg tattgatcgt agcacaacgt gtgggaacga
    11281 tcatgcatgc agataagatc atcgtattaa atgaaggaga agtcgtcggt atggggacgc
    11341 atcgggagtt gttggaaaaa tgcccgattt attatgatat tgccgcttct caattatcaa
    11401 aggaggaatt agcatgaaaa aaaccttttc ttccttaaaa cgtttagctc gatatatcag
    11461 accgtatcgt gagacttttc ttttagtgat cctatttaca gttctgacag tggctttcaa
    11521 cgtggctttg ccatatgtta caggacttcc tactacagaa atcagccgta atattgccaa
    11581 tggcgaatca atcaattttt cttatatatt caaatgtctc atttggatca cagttgttgg
    11641 aatagggtat tgtatttcgc aattactttc tggtgttttg atgacaaatg tcgttcaaag
    11701 tgccatgcat gatttgagaa gagacataga agaaaaaatc aatcgtcttc cggtttcata
    11761 tttcgataaa aaccagcaag gaaatatttt gtctcgtgtg acgaatgatg tagatgcagt
    11821 cagcggtgct ttacaacaag cttttattgg tatcgtaaat gcgatattgg gaattacgtt
    11881 ggctgcagca atgatgtttt atatccagcc gatgatggcg ctgatctcta tgatcatgat
    11941 tccaacatcg atctggatct caaaacgagt gatcaatgct tcgcagaaat attttcaaag
    12001 catgcagaat tctcttggtg agctaaacgg ctacgtccaa gaaaatatga caggttttag
    12061 tgtattgaaa gtatatggac gcgaaaaaga aacattggaa ggattcaaaa aagttaatca
    12121 cagcttgaaa tactttgggt ttcgtgcagc attcatttct ggcttgatga tgcctctagt
    12181 tcaattaact gcctatgcta cttatattgg tatggctgtt ctaggaagct tttatgttat
    12241 tactggggcg atcgttgtcg gtcaactaca agcattcatc caatatattt ggcaaatcag
    12301 tcagccaatg gggaacgtga cacaattgtc ttcagttttg caaagtgcat cagcggcaac
    12361 gaaacgtgta ttcgagattt tagatgagcc agaagaaaaa gaaaacaaag tagatgcttc
    12421 actgccagaa acgattttag gagatgtgac attcgatcat gtcgattttg catatgatcc
    12481 gaagaaacca ttaatccaag atttgaattt tgaagtgaaa gctggtcaaa ccgtagctgt
    12541 ggtcggtccg accggtgccg gcaagacgac attgatcaat ctattgatgc gtttttatga
    12601 cgtagacaaa ggagcaataa ggattgatgg aatcgacacc aaatcaatga gtcgcagtga
    12661 tgtgcgttcg ctatttggta tggtcttgca agatgcatgg ctttatcaag gaacgattgc
    12721 agataatatc cgcttcggaa agcttgatgc gacagattat gaagtagttg atgctgcgaa
    12781 aacagcaaac gtagatcatt ttatccgaac gatgccagat ggatatgata tggccatcaa
    12841 ttctgaggga gacaatgtgt ctttaggaca aaaacagttg ctgacgattg cccgagcagt
    12901 ggtctccgat cctaaaatct tgattttaga tgaagcaaca agttcagtag acacccgttt
    12961 agaagcgttg attcaaaaag caatggatcg cgtgatggag ggacggacaa gtttcgtcat
    13021 cgctcaccgt ttatcgacaa tccgtgatgc agatctgatt ctagtgatgg atcaaggtca
    13081 gatcattgaa aaaggaacgc atgagagttt attggcacaa ggtggatttt atgaaaaact
    13141 gtataacagc caatttgctg aggaagcaga ataagtcata taagaataaa acagtttaaa
    13201 acttattttt tgttctctat tcttgataat ggtaaagtaa agttatcaag aaaagtgagg
    13261 acacggagat ggaacagttt ttaaatgaaa aaattactga agccaaaaga gaagttgatc
    13321 gtttaacgca cgtctgtcag acgaagcctt ttgattcagc tgattttttt gaaaatgagt
    13381 ttcaaatcga acgcttggca gcacaaatag aagtgtacga attaatttta aaaaaagtaa
    13441 tagaagaaaa tatagatgta caataaaaac aggtaaaacg tcagttacca gatactgtaa
    13501 atagacatct ggcctgagca ggttttacct gttttttttc ttatgaaata aatatatgct
    13561 atgatacctt tattcaatag gattagtttc tacaaaaaat gaaaggagaa aaataaatat
    13621 ggaacggttt atagcatcaa atgccacagt gattggtgat gtgacattgt cagaagatgt
    13681 gacgatctgg tatcaagctg ttttacgcgg ggacagtaat tggataaaaa taggacaaag
    13741 aacgaatatc caagatggta caatcatcca tgtcgatcat gatgcacctg tagatattgc
    13801 ggagaatgtg acggtaggac atcagtgcat gctacatggc tgtacaatcg aaaaaggggc
    13861 gctgatcggg atggggacca cgatattgaa tcatgcggtt attggtgaga atagtttgat
    13921 tggtgctgga tcattagtaa cagaaggaaa agttattcct cctaatgtct tggcttttgg
    13981 tcgcccagct aaagtgatcc gtcctttgac aaaagaagaa atacaaaaaa acaaagaaaa
    14041 catccagcat tatgtcgaga taggtcggat gcatgcgcaa ggaaaattca aggagcaaag
    14101 ataggcagta gcatccataa aataaaaaag gatggtcagt ttgagttgac catccttttt
    14161 tatttggttt agataagaga gcttaaaaaa gcttcctttg aaacagatta atttaatgaa
    14221 gaactgtttt ccatgacttc atcgatcaat ccatatgctt ttgcttgttc agctgtcata
    14281 tagttgtcac gatctgtatc tttttcaatc acttcaagcg gttgtccagt gcgttcagct
    14341 aaaattttgt ttaagcgttc gcgtgtttgc aaaatatggc gtgccgcaat ttcgatttca
    14401 gttgcttgcc cttgagcgcc gccaagaggt tgatggatca tgatctcagc atttggtaaa
    14461 gcaaatcgtt taccttttgt accagctgtc agcaagaagc ttcccattga agcagccata
    14521 cccataacaa ttgtttggac atctgctttg acaaagttca ttgtgtcgta aatagccata
    14581 cctgctgtaa ctgaaccacc aggagaattg atataaagat agatatcttt ttcagaatct
    14641 tgagcgtcta agaaaagcaa ttgggcgatg atcgaattag caagatcatc tgttacttgg
    14701 ccgcttaaca tgatgatacg gtctttcaat aaacgtgagt agatatcgta agctctttca
    14761 cctcgtgaag attgttcaat gactgtagga attaaattca taatagagtt cctccttgat
    14821 ttcgtttaga aatagtgtac cataatttag tttctaatac ctaaaatgta gtatacattt
    14881 ttggtcaaaa aaggtcaaat aaaaaactta ggcaaattag tcgcataaac ccgctttatg
    14941 gtatgataag cagagaaaca aaacaaggag gaacaacaga ttgatagttg gtattactgg
    15001 ccttatcgga gtgctgacag gtgctgtagt tgtctatatc ggcatgatac tgcaaatgcg
    15061 tcacttggaa aataaagaac agcgtagacg tgaattggaa ttgaaagtca aagaaatcga
    15121 gacgctgaat caattaaata aaaagatcaa cgagatcttg caaaaacgga caacgctgat
    15181 ggaagaatat gtcagctttg atgcatttga tgattgttat atcacgattg acgattttgc
    15241 gtatctccaa tcattttcag cgcaaaatag tttttatctg ccaaattata ttttagacga
    15301 gctatttaag aatatctccc atcgaaaagt gattttatct ccagatgaaa caatgaagat
    15361 tggtggatat gcttataaag gcggacgcgt gattttagag aattttacgg atagtctgac
    15421 tgaaatgatc aatgaaaaaa aagctcagtt gaagactatg acgcgccagc cgattggttt
    15481 cttctcgaaa gatgatgttt ggagctgata ttttgaagca ttgccgttgc acatgagagc
    15541 gtggtaatgc tttctttttt tcttaatgat acaatagaac tgaacggagt gaagaaagat
    15601 gttacaaatc tatgcccatc gcggaagcag cggcacccac ccagaaaata cgcttcctgc
    15661 atttgcagaa gctgtacgag taggtgcaga tggtatcgaa ctagatgtcc atctaagcaa
    15721 agatggttat ttgatcgtta tgcacgacga agaagtggac cggacgacta acggtaaagg
    15781 gctgattcga gaaaaaacat tggaagaact caaaaaattg aatgcagggg gcaaattcag
    15841 caaagaattt ccagcagcta aagttcctga attgagagaa gtgttaggac tgctcctcca
    15901 aaaaaattac cgagggatgc taatgattga aattaaaact gatcagtatg aatatcccgg
    15961 tatcgaagaa aaattagccg aaacattaaa aagtagagat tggccttttc aacatgcata
    16021 ttgcagcttt catttggatt ctttggaaaa aatcgcatta ctggaaccgg agatacagct
    16081 ggacttattg atgagtactt ctgataaaaa gatccaacta gcagcagaaa ccccttatat
    16141 agaaggagta catccaaaaa atacttggtt gttcgaacat ccagtccagg cagctgattt
    16201 tccaaaagca attcgtgcat ggacgattaa cgatgaagaa gaaatggcca aatgtcaaga
    16261 actcaaaata gatggaataa taactgattt tccggagaaa gctcttcggt ggaaacaatc
    16321 aacgaaaagt aggtaaaata tggaaaaagt attagtgatc gtcggtccga cagcagtcgg
    16381 taaaacagcg ctaagtgtgg aactcgcaca actatttgga ggagagatca tcagtggtga
    16441 ttcattgcaa atctataaaa atctggatat cggtacagca aaaatcaaag aagaagagat
    16501 gaataatgtt ccgcatcatt tgattgatgt gatcgaacct actcaacgtt actcagccgc
    16561 tgattttcag cattcaggtc gacaattgat taccgagatt gcgaaccgag ggcacctacc
    16621 tatcatagct ggtggtacgg gtctctatat ccaatccctt ctatatgatt atcagttagg
    16681 agcgacagaa gaagcgaatc ctgagattag aaaaaagtat gaaacgttag ctgaaacaat
    16741 tggttcccaa gcattatgga atcagttgaa agaaaaagac ccggcagctg ctgagaaaat
    16801 ccactggaac aatcaacgca aagtgataag agctctggaa gttttagaga cgacaggaca
    16861 tagtatcacg gcacctaaag aagagccaaa gcaattgtat gattacctca tgattggatt
    16921 agatacgaag cgtagtattc tttatgaacg aatcaatcaa agagtcgatt tgatgctagc
    16981 agaaggttta gtagcagaag cgcgtaaggt gtatgagctt ggagaagtcc aggccagtca
    17041 aggtattgga tataaagaat ttttccctta ttttgaaggg aaagaaacat tggaagaagc
    17101 aacagaacaa gtcaaactac attcgagaag atacgcgaaa cgtcagttga cgtggtttcg
    17161 taaccgtttg gatgcacagt ggtttgatct tataagtgag ccaaatcaaa aaagtcagat
    17221 tgagcaaaca atcaaaaaat ggctggaggc ggaataaaat ggaagaaaaa gtgatcatcg
    17281 taggggtaga aaccgaagca aaccagcgtt acttttcaga gtcaatggaa gagctagttc
    17341 agctgacgaa taccgcatca ggagaagtcg tattcacaat tacacaaaag agaccacaag
    17401 tggatcggca aacgattatc ggtaaaggga aattacaaga attagttcaa caagcggatg
    17461 cgcatgaagc ggatttgatc atctttaatc atgagctaac tccgagacag agccaactga
    17521 ttaccgatgc tgttggtctt ccggtaattg atcgcgtgca gcttatctta gatatttttg
    17581 cgatgcgtgc tcgttcgaaa gaagggaagc tgcaggttga acttgctcag ttagaatacc
    17641 tgctgcctcg tttagtaggg caagggaaaa cattatctcg tcttggcggt gggatcggaa
    17701 caagagggcc aggtgaaaca aagttagaaa cagaccgccg acatatacgg aataaaattt
    17761 ttgctgttaa gaaagaattg aaagaagtag aagctcatcg agagcgcaat cgccaaaaaa
    17821 gaaaaaatag cgagattttt cagattggac ttattggcta cacgaatgcc gggaagtcaa
    17881 ctatcttgaa tctattgaca caagcggata cttattcgaa agatcaattg tttgcaacgt
    17941 tagatccatt gactaagaga tggcgttttg ctgaaggatt tgagattact gtaacggata
    18001 ccgtcgggtt catccaagac ctgcctacgc aactgatcga tgctttccat tctacattag
    18061 aagaaagtca aaatatggat cttttgctac atgtagtcga tgctagttct ccagatcgta
    18121 ttttacagga gcagactgtc ttaaaattga tggatgaact gaatatgaaa gaaatgccga
    18181 tactgactgt ttacaataag gcagaccaga tcgacccagc gatgttcacg cctacgctat
    18241 tcccaaatgt attgatttct actcagacga aagaaggtaa gatggcgttg atcgaagcaa
    18301 tcaaacgtca gctgatggaa ctaatgaccc cttatacact ttctgtgcca agcaacgaag
    18361 gacagaaatt aagcgagttg agaagacaaa caatggtttt ggacgaagcg tacatggaag
    18421 aaacaaacga atatgaagtt aaagggtttg caaaaaccaa ttcaaaatgg ttaagaaaaa
    18481 accaataaaa ttttattttt ttaa
//
LOCUS       DACEUZ010000045        18356 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.045, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000045 DACEUZ010000000
VERSION     DACEUZ010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18356
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.045"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            9..851
                     /locus_tag="IXG44_001339"
     CDS             9..851
                     /locus_tag="IXG44_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAQ1658069.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVTVVLFFKELLIT
                     SFDPLMAKAMGMNVSFYHYLLMILLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGACSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERKL"
     gene            848..1798
                     /locus_tag="IXG44_001340"
     CDS             848..1798
                     /locus_tag="IXG44_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAQ1658070.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSTNSKDKDTVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            1813..2046
                     /locus_tag="IXG44_001341"
     CDS             1813..2046
                     /locus_tag="IXG44_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HAQ1658071.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            2377..2961
                     /locus_tag="IXG44_001342"
     CDS             2377..2961
                     /locus_tag="IXG44_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1658072.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLV
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            3092..3760
                     /locus_tag="IXG44_001343"
     CDS             3092..3760
                     /locus_tag="IXG44_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAQ1658073.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFGTSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            3762..5186
                     /locus_tag="IXG44_001344"
     CDS             3762..5186
                     /locus_tag="IXG44_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ1658074.1"
                     /translation="MKELFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSG
                     KSTLLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQNPRGQFFTTN
                     TTSELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLS
                     LNSKLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVK
                     NRTVKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDIN
                     FDVKVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQF
                     FTESVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSK
                     TNLFLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGG
                     IKKDIKLRVEDSTLIEQIFNDITK"
     gene            5204..5773
                     /locus_tag="IXG44_001345"
     CDS             5204..5773
                     /locus_tag="IXG44_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HAQ1658075.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVVSWVLMQLGSAAGQWIPIWFFTDRFRQDWIDRGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            5883..7637
                     /locus_tag="IXG44_001346"
     CDS             5883..7637
                     /locus_tag="IXG44_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1658076.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQYLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHRVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            7591..9363
                     /locus_tag="IXG44_001347"
     CDS             7591..9363
                     /locus_tag="IXG44_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002841847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1658077.1"
                     /translation="MRMQMIGGLTMKNFYKKKFALTDQGAEALTKASISSFFVYCINM
                     VPAFIIMMLIDELVLENAKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLR
                     VQIADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVM
                     LLVGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEI
                     NSFNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLL
                     TGEVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSP
                     LKSFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDY
                     DEGCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAA
                     RLANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLD
                     AENEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSE
                     IYKNLIKKTKLSEKFVYEKEAQSR"
     gene            9886..10155
                     /gene="rpsN"
                     /locus_tag="IXG44_001348"
     CDS             9886..10155
                     /gene="rpsN"
                     /locus_tag="IXG44_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAQ1658078.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            10170..10349
                     /gene="rpmF"
                     /locus_tag="IXG44_001349"
     CDS             10170..10349
                     /gene="rpmF"
                     /locus_tag="IXG44_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAQ1658079.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            10381..10758
                     /locus_tag="IXG44_001350"
     CDS             10381..10758
                     /locus_tag="IXG44_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1658080.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            10779..10928
                     /gene="rpmG"
                     /locus_tag="IXG44_001351"
     CDS             10779..10928
                     /gene="rpmG"
                     /locus_tag="IXG44_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003550836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAQ1658081.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            10952..11935
                     /locus_tag="IXG44_001352"
     CDS             10952..11935
                     /locus_tag="IXG44_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAQ1658082.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            11965..12087
                     /locus_tag="IXG44_001353"
     CDS             11965..12087
                     /locus_tag="IXG44_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAQ1658083.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            12105..13628
                     /locus_tag="IXG44_001354"
     CDS             12105..13628
                     /locus_tag="IXG44_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAQ1658084.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            13652..13891
                     /locus_tag="IXG44_001355"
     CDS             13652..13891
                     /locus_tag="IXG44_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1658085.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(14084..14545)
                     /locus_tag="IXG44_001356"
     CDS             complement(14084..14545)
                     /locus_tag="IXG44_001356"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ1658086.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(14740..14958)
                     /locus_tag="IXG44_001357"
     CDS             complement(14740..14958)
                     /locus_tag="IXG44_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1658087.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            15076..16692
                     /locus_tag="IXG44_001358"
     CDS             15076..16692
                     /locus_tag="IXG44_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAQ1658088.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            complement(16933..18273)
                     /locus_tag="IXG44_001359"
                     /pseudo
     CDS             complement(16933..18273)
                     /locus_tag="IXG44_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT         6304 a         2620 c         3516 g         5916 t
ORIGIN      
        1 ggaataatat gcttcaaaat tttattaatg gtctgtatga ctttcacttt ttacagaatg
       61 ctctaattac atctgtagtt atagggattg tttctggaac agtaggttgt tttattattt
      121 taagaggaat gtctcttatg ggtgatgcta tttctcatgc tgttttgcca ggagtagcaa
      181 tttcttatat tttaggtatt aattttttta ttggtgcgat tgtatttggt ctattagctt
      241 ctatcattat tacttttata aaaagcaata gtgtaataaa aggagataca gcaattggta
      301 ttactttcag ttctttttta gcattaggag ttatccttat aggagttgca aatagctcaa
      361 cggatttatt tcatatctta tttggaaata ttttagctgt acaagatatt gataagtgga
      421 ttacaattgg cgtttctgtt ttagtgttgg ttacagttgt tctatttttt aaagaattat
      481 taatcacttc ttttgatcca ttaatggcaa aagcaatggg gatgaatgtc agtttttatc
      541 attatctatt aatgattcta ttaactttag tagcagtaac agctatgcaa agtgtgggaa
      601 ctatattaat tgttgcaatg ttaattacac cagctgctac agcatattta tatgcaaaca
      661 gcttaaaaac tatgatattt atttcggcga cagtcggagc gtgctcatca ttgctaggtt
      721 tgttcattgg ttatagtttt aacatagcag caggatcaag tattgtttta acagcggcat
      781 tcttatttgt aattagtttt ctaatctcac caaaacaaaa atttttaaga aagaaggaga
      841 gaaagttatg atgagaaaat ggaaagcagt actgggaagt ctggggattt tgattgctct
      901 ttttatattc ggggcatgtt caacaaatag taaagacaaa gatacagtgg cttcgaacga
      961 aaaattaaag gtagtagtta ctaattcgat tttagcagat attactgaaa atatagcaaa
     1021 agataaaatt gatttacaca gtatcgtacc tattgggaaa gatccccacg aatatgaacc
     1081 tttgcctgaa gatgttcaaa aaacttcaaa agcagatttg attttttata acggtgttaa
     1141 cttggaaact ggaggaaatg cttggtttac aaaattagta aaaaatgcga acaaagagga
     1201 aaacaaagac tattttgcag caagtgatgg catagatgtt atttacttag agggtcagag
     1261 tgagaaaggg aaggaagatc cccatgcttg gttaaattta gaaaacggta ttatttacgc
     1321 taaaaatatt gaaaaacagt tagcggaaaa agatcctgat aataaaaaat tctataaaga
     1381 aaatctagat aagtatattg aaaaattgga ttctctagac aaagaagcta aatctaaatt
     1441 tgcttcaatt ccgaatgata aaaaaatgat tgttacaagt gaaggatgct ttaaatattt
     1501 ctcgaaagcg tataatgtgc cttctgctta catttgggaa atcaacactg aagaagaagg
     1561 aacaccagat caaataaaac acttagttga aaaattacgc acaacaaaag ttccctcctt
     1621 attcgtagaa agcagtgtgg acgatagacc aatgaaaaca gtatcaaaag ataccaatat
     1681 tcctatctat tcaacgattt ttactgattc aattgcagaa aaaggacaag atggtgatag
     1741 ttactatgcg atgatgaaat ggaacctgga taaaattgct gaaggccttt cgaaataatt
     1801 tggagggtag gtatggtaca aattaaaacg actactatac taatttattt actagcaatt
     1861 caggaattat caaaaagaaa aaagaaatta aaaaataatg atttagcaaa agttctaagt
     1921 gttagtccag catctgtaag tgagatgctg gctaaactta gatatgagga ctatttagat
     1981 ataggatttc aattaactga taaagggaaa aaatttattg acgattacaa aaaaggtttt
     2041 aagtaaaaga ctttattttt agggaggata agaaaatggc tattggagtg attatgatag
     2101 gacttaccct aatagctgga tataattttt ttaaatcttt ctcggattat tttagaaaag
     2161 gtgaatgatt tagatgagtc tattaattct tgtaggattg ctttttgtat cagttatttt
     2221 gctctttctc attagtttag ctctaaggaa tgagttcagt aatagacgtt aacgattgaa
     2281 acttcaagtc ctgtgtaaga taatatagtt aattcatctt attaggagga agtacccata
     2341 gaaaccagag attatattac aactcaccaa aaattaatag aaagtggact aagccatttt
     2401 gaaaaagaag gatttcagca aactaagata aggcaactgt gcttgaccgc aggcgtaact
     2461 acgggtgctt tttataaaca ttttacttct aaggaagcgt tatttgaaga aattattcag
     2521 ccgcatatta atgcgcttat taatatgtat aatgaagagt ttaccaaata cctcttaaac
     2581 ttagaaaata tagatataga gaaaagttgg agagagaact tcaatgaatt agatccattt
     2641 attaattata tttttgagaa taaacgtata ttcgatttgg ttttattcca atcagctgga
     2701 accaaatatc aagatattgt tgaaaaaatt acatcatttg tgacaaaaaa gacagtctgt
     2761 tgtctagata agttaaagac tatcggaatg atcaatcaaa atttagtctt taatgaagaa
     2821 gaaatccact tctacattta ctcattctac gctacattct atgatatatt gaagcatagc
     2881 tattcaaaag aaaagactat gctattaacc agaagattgt atcaattttc tgttcctggg
     2941 tggattgaat tactgagtta attaaaaaaa agcttgcatt gagaattgtt ctcagttatg
     3001 ctttttgata agatcgcact gttatctgaa taacggtgca tttttgtcag cttaagataa
     3061 cagtgcgatc ttattttttg gaggtgaaag agtgtcaaaa atcaatccgt taaccttgat
     3121 tactttaaat attttttttc cggtcattat ttttttagga aagggaaata tatttcaagt
     3181 ggggtgtttc attattgcta cagttttagt attgatgttt cgtaaaataa agacatgtat
     3241 ttatttcatc ttgatttatt cagttctgtt tttagtaatg aaaattattg atgctatcaa
     3301 gatagagttt tttactatat tttttgggac atccttatat attgtatttc gaatgttgcc
     3361 ggtcgtaatg atctcatggt tgttggttag tgtctatact agcaatgaat tattatcctc
     3421 tttagaaaaa atacatttac cgaaaaaaat aatgttaagt attacagtag tgatcagatt
     3481 ctttccgact tatcgttctg aaataagaat gattaaagaa tctttaaaaa tgagaaacat
     3541 tcgcttgtct ttttttcagc ctctgcaata tttggaattt ctgttagttc ccgttcttat
     3601 acgtgcaact atgattgctg aagaaatgac cgctaatgca ataacaaagg ggatagaatc
     3661 tcctgtgaaa agaagttctt actataaaac aaacatgagc ttattagact atatcatcct
     3721 gttttccgtt actatttttt tcttaagtat tttgatttga ggtgaaagaa ttgtttgaat
     3781 ttaacaatgt ttctttttca tataaggatg ttaaggaaca agcgcttgtt gaccttaatt
     3841 tttcaataaa aaaaggcagt cttacattga ttactggttc ttctgggagt gggaaatcca
     3901 cactattaaa gttaatgaat ggacttattc caagacttta tgacggaaaa ttggttgggg
     3961 aaattttatt tgaaaataac tcgctacaat ccttaacaca agaaagtata tcaaaaaata
     4021 ttggctacgt aagtcaaaat cctagaggac aattctttac aacaaataca acgagtgagt
     4081 tagtattttc gatggaaaac tttggattta caaaagagca tatggataaa agaatcaatg
     4141 aattagctga attattagat ctttcaaata tattagataa aaacattttt tcgctatcaa
     4201 gtggtgaacg gcagaaaatt tctattggat gtagtttatc gctaaactct aaattaatta
     4261 tattggatga accgtcttca aatttggact tcaaagcgac taagaaactt gctgacttac
     4321 tttgtgattt aaaaaataaa ggatttacta ttgttatagc tgaacatcgt ttttactatt
     4381 tgaaagaatt aattgatcaa gttctatttg ttaaaaatag aactgtaaaa actacaacta
     4441 ttcagttgtt aaaaactgaa aacaataacg cattaaggaa atttgacatt tttaatagtt
     4501 cgattgaagt tccctatatc gaaaaggtaa gtgcagatgt attaaaagtt gaagaaatta
     4561 cttatgaaaa tattctaaaa gacataaatt ttgatgtcaa agttggagat gttattggtt
     4621 tggtagggaa aaacggtgtg gggaaaacaa ccttactaag aatactcatg aagattattc
     4681 gccctaccaa aggaaagata atcgaaaata aaaaaaattt gtcatcgccg tttttagtaa
     4741 tgcaagaaat ggattaccaa tttttcacag aatcagtgag gagcgagtta gccctgggga
     4801 atgaaatagt tagtaaagat cagcaagaaa aaattttaaa aaaaatggaa ctttataaaa
     4861 tgaaagatca attaccgttt gatttatccg gtggagaaaa acaaagatta ttggtttcta
     4921 tagcttcatt gtcaaaaact aatttatttc tatttgatga acctacaagt gggttggatt
     4981 atatcaatat ggaaaggatt gctgacctta ttaaagactt aaaagaaaag ggtgcagtag
     5041 ttatagccac tcatgatccg gaatttcttt ataaaacatg caatagaatt atttatttaa
     5101 aaaatggtgg aataaaaaaa gatataaaac ttagagtaga agattcaact ttgatagaac
     5161 agatttttaa tgacataacg aaatagaatt gaggagaata tatatgaaaa caaaagatta
     5221 tatttttatt ggtttagcta cagttatttc cctagtaatt tacttttttt caataatggt
     5281 tagtagtatt ggtggggcgt ttggtcacag catatcccca gggatttttg gattattatc
     5341 tggaatcata tttgtttata ttagctataa ctaccctaga aaaggaattt ttactattta
     5401 cacaattgtt ttattgttat tttttacgtt aatgggcggg gcgtacttac cttggtttat
     5461 cagttcgatt agtatggcga tcttagctga tattattcta tctgtatttg gatatgatcg
     5521 agcaattcct caagttgtgt cttgggttct aatgcaacta ggcagtgctg ccggacagtg
     5581 gattcccata tggttcttta cagatcgttt tcgtcaagac tggatagata gaggacaatc
     5641 tgctgcaaca atggatgcaa tgatccacta cgcggtgggt atatggggaa ttatttcagt
     5701 gttagttgta gcaagtttat ctatgattgg tgtattgatt ggaagaaaag tcttaaaaaa
     5761 atacaaaaaa tagaacgtag tttaccttct atctgtttgt acatctctaa tttacgctat
     5821 tacggaaagc attaacagtt gtaaacgaat aagagaagaa attgaatgcg ggggaggaaa
     5881 aaatgaaagt atacaaaaaa ttatttagtt atgtccctaa aatgaaatac tacggatggt
     5941 tagccacttt attttctgat ttttctgtcg tattaacagt ctatggatat tacagtattt
     6001 acaaatttct tcagtctcta attattgtag gagatgaata tttagctaag agttatgcgg
     6061 ttcaaactgt tgcttggtta actttaggag ctttatttta tattttttct ggcttatttt
     6121 ctcatatcct tgggtttcgc ttagagacta atttgagaaa aaagggaatt agcggtttaa
     6181 cagaagcaag ttttcgtttt tttgatttaa attcttcggg atatattcga aagacaattg
     6241 atgacaacgc ggctaaaaca catatggcaa ttgctcactt gattccagat agtgctcaag
     6301 ccatagtaac acctgtttta tcagttgcct taggcttttt cattaacttt aaaattggat
     6361 tggttctaat cgccatgtta gtaattggtg tattcttgtt aaagaaaatg acagagaata
     6421 caaattttat tcaaaaatat caagaatcgt tagatgtgtt aagctctgaa acggtagaat
     6481 atatccgcgg aattcaagtt ataaagatat ttggtgctaa agtaacatca tttaaagcct
     6541 tgaataaagc aattaacgat tatgctaagt ttgcatataa gtattctttg agtgggaaaa
     6601 caccttttgt tctttttcaa tggctatttt ttggcatagt ttgcattttg atagttccta
     6661 taaccttttt tataacttct cttaccactc ccaagatctt agctgttgac ttgttgatgt
     6721 tgtttttttt aagtggaatt atttttgttt ctttcatgag aataatgtat gtaagtatgc
     6781 acttgtttga agcaagatat gcagtcgaaa cactagactc cctttattta gaaatgaagc
     6841 aagaccaact aaaatatggg aacgagacac agattaaaaa ttgtgacatt gcatttgaaa
     6901 atgtcagctt ttcatacaaa gaagagtgtg ttttagaaaa tttcaactta gttttagaac
     6961 aaaaaagaat ttatgcatta gtaggaaaat caggaagcgg aaagagtacg atagctaaac
     7021 taatttcagg tttctacaat gtggataagg gacagattaa aattggtggt aagcctttaa
     7081 atcactattc aaaagacaca attattaaca caattgcttt tgtctttcaa gatacgaaac
     7141 tattcaatca gagtatttac gataacgttt ctatagcaaa tcctcaagcg ccagcaagta
     7201 aagtatttga cgcgctaaga ttagccggtt gcgaatcgat tttagaaaaa tttcccgaaa
     7261 gagagcatac cttaattggt agtaaaggag tttatctgtc aggaggagaa aaacaacgta
     7321 tagctattgc cagagcaatt ttaaaagatg caaagattgt tattatggat gaagccagtg
     7381 catctattga tccagataat gagtatgaac tacaaaaggc ttttcaatat ttgatgaaag
     7441 aaaaaacagt tattatgata gctcatcgat tatccagcat taaatctgta gatgaaattc
     7501 ttgtcttgtc agaaggaaaa atagtagaac gtggaaatca taatgaatta atggctaaac
     7561 atcgagtgta taaacaattg gtcactaatt atgagaatgc aaatgattgg aggcttgaca
     7621 atgaaaaatt tttataaaaa aaaatttgct ttaactgatc agggcgcaga agctttaact
     7681 aaagcttcta tttctagttt ttttgtatac tgcattaata tggtaccagc attcattata
     7741 atgatgctga tagacgaatt agttttagaa aatgcaaagc cccgttggct ttattttgca
     7801 gtatcgtttg ttactttgct tttcatgtat tggttgttag atagggagta tgaaaactta
     7861 tataatagta cttataaaga aagtgcgcat ttaagagtgc aaattgcaga cgatttgtca
     7921 aatttaccat tatcctattt ttcaaaacat aatttatcag atttatctca aactatcatg
     7981 tctgacgttg aaggtattga gcatgcgatg agtcatgcaa tacctaaagc cggcggtatg
     8041 gctctgtttt tcccttttat ttcagtgatg cttttggttg gtaatgtcaa aatgggatta
     8101 gctgttattt tgccaacgtt atttagtttt gtcttaatct tgttatcaaa gaaatcccaa
     8161 acgaaagcca atactaaata ttacgatact ttgagagaaa actcggaaga atttcaagaa
     8221 actattgaat tgcagcaaga gattaatagc tttaatctat ctaaaaaagt tcaagacaga
     8281 cttttcaaaa aaatggaaga gagtgaaagg attcatttaa aggtagaatt aagtactttt
     8341 tcagtcatgg ccttatcctc tattttctca tatgttagtt tagcagtagt cattctagta
     8401 ggtgttcact tactgttaac gggtgaagtg actatactct acgtcgttgg ttacttacta
     8461 gccgcaataa aaataaagga ttccttcgat tctatgaaag aagcagtgct tgaaatattt
     8521 tatttagctc ctaaaataca gcgaattaga gctatgaagg aaaccagtat ccaagaggga
     8581 tcagatagtc ctctgaaatc ttttgacatt gagttgcgag atgtctcttt ctcatatgat
     8641 aacaataccc caattttaga tcatatctct tttacggcta aacaaggaga agtgactgcg
     8701 ttagtaggtg ctagtggttc cgggaaaact agtatcttga aattagtgtc tagactgtat
     8761 gattatgatg aaggctgtat tttaattgat gggtatgata ttaaacgtgt atctccagct
     8821 tctttgttta gcaaaatcgc tattgttttt caagaggtaa ctttgtttaa tacaagtatt
     8881 ttggaaaata tacgtattgg aaatagtcaa gcgtcagatg aggaagtgaa aaaagcagct
     8941 cgtttagcta actgcgaaga ctttattgaa aaactacctg acggttatca tacattggtc
     9001 ggagaaaacg gaagttcact ttctggagga gagcgacaac gcctatccat agcacgggct
     9061 tttttgaaaa atgcgccaat tttaatttta gatgaaatta ctgccagttt agatgcggaa
     9121 aatgaaaaac gtattcagga aagtttaaat cgtttaatac aagataagac agtactaatt
     9181 atatcgcatc gcttgaagtc tattgaaaag gtaaataaaa ttgtagtgat ggatcaagga
     9241 aaactagttg atcaaggtac tcataatgag ttgtatcgaa ggtcagaaat atacaaaaat
     9301 ctcataaaga aaactaaact atctgagaaa ttcgtctatg aaaaagaagc tcaatcacga
     9361 tgatgaaata ggctttaaat taacctacaa tttttgggat atagttataa ttctttttca
     9421 aaaagaataa ttttattaaa cagcatctaa ttttgaatac acgaaagaat cagtatgatt
     9481 gaattagttc acttaatgtc tattcttata atataaggaa taatcaaagt tttaaaaaat
     9541 tggcgataaa ccagttaaac ataaaagatt gaataaaaag ttatagtttc tttcatattg
     9601 ccgttttaag tagaggaaaa gctacttaaa acggtatttg tgtttgattg gactaataaa
     9661 agtgaattct tatttttcag agcctaaact gcatattctt ttaaatgatt tgattatctc
     9721 tatgtaccga taaattcgtt ttcaaatagt tatctattca tatgagtttt atttcatgtt
     9781 atttattgtt gacaagagca actcttgttg gtaaagtatc agtgtcaaaa cgtaacgatt
     9841 acgatttgag gtgtctaaat caaaataaaa ggagtataaa ggtatatggc aaaaaagtcg
     9901 aaaattgcta aagcaaagaa acaaatggcg atgattgaaa agtatgcaga taaacgtcag
     9961 gaattaaaag ccgcaggaga tcgcaccgct ctcgctaaat tgccaagaga ttcaaatccg
    10021 aatcgtctga ggcttcgtga tcagacggat ggtcgtccaa gagggtatat gcgaaagttc
    10081 ggtatgtccc gtattaagtt tagagaactt gcccatcaag ggttgattcc tggagtaaaa
    10141 aaagcaagtt ggtaaaagga ggaaatatca tggcagtacc agcaagaaaa acatctaagg
    10201 cgaagaaacg gttaagaaga actcatcaaa ctgtagtaaa accagaaatt tcatttgacg
    10261 aagcgaatgg tgattataga cgtagccatc atgtatcttt aaaagggtac tataatggaa
    10321 agaaagtaat taaaggagct tcaaaatagt ttaatatata acttttagga gggaatcaaa
    10381 atggaaatta tttacccacc tttagttgaa gaaggattaa agtattatct tgaaacgacc
    10441 cagcaatcgc tagataaatg cactttttat cgttcgatgg tagagagagg aattattact
    10501 gaaacgggtt taccgaccca acaggcaatt gaaaatggat tagtcaaaga ttactatgag
    10561 gaccaaggct tatcatttga tgatttttta agaatctatc caatttttga agagtatgac
    10621 gaggagttat ttcaatgtat cgatggttat tgggagatac ctgttgatat gaaagaaaat
    10681 ttagtttcac aattggaatc tggcgagtta acttttgaag atgcacaaca aattcaagca
    10741 tatcttgaag atcgataagt attgaaggag gaagatttat gcgtcaaaat attattttgg
    10801 aatgtgtcga aactggagaa cgtctgtatc tcaccagcaa aaataaacgg aataaccctg
    10861 aacggttaga attaaaaaag tattcgccaa aattgcgcag acgggcaatt tttaaagaag
    10921 tgaaatagct aagtggtaaa aaggtgacaa aatgggcata ccagttacaa tcgtttctgg
    10981 gtttttaggg gctggaaaaa caacgctaat caatcaagcc ttgcaagtct caccctaccc
    11041 cagggaagaa attattataa ttgaaaatga gtttggtgaa gttgggattg atcacaaatt
    11101 attagttcat agcgaggaac gggtgttaca actaaataat ggatgtatgt gttgcagtct
    11161 tcgcagtgat ttattagcaa tattgatttc tttactggaa acaatagaag aacatcaaca
    11221 accaatttca caaatcatca ttgaaactac aggtatagca gatccccaac caatcattca
    11281 aacactttta acagcgcctc aactaagagg aagagtatat atcgatagtt tattgactgt
    11341 tattgatagt gacagttttg aacttattga aagagaacca caagccctta agcaacttgt
    11401 catggcagat cgcatttttg tgtctagaaa acaaaacttg aatttatctg cactaaaaaa
    11461 attacagaga gaaatcaaaa gtattaaccc tttatccgat attcgaatgt tcacacatag
    11521 tgaaacgatt caaaaaaagg atttttataa tttgaaaaaa tttaatcatc ccttagtttt
    11581 tgaaagtgat tcatcagaaa atcactataa tggcaaatac tatcatcaac accatcaagt
    11641 acatgagttt aaatcaattc tcttaacttc agatcaagac ataaaaaaag attttttact
    11701 ccaatggatt gactggctga ttttaaccaa ccaagggagc ctgtatcgtt ttaaaggatt
    11761 gattgcttta caagagcagg attttatagt tgccatgcaa gaggtaaacg aacaggttaa
    11821 ttttcaatac accactcgac aaaaagacca aaccactatt attctaatcg gaaaaatgtt
    11881 ggatgaagaa aggatcaaac agagttttga taaattactg gcggaaagca aataatgata
    11941 agtagtgatt gggggtaatt tctaatggag aaaacggata tatctagtgc atatcgacga
    12001 cttaaaagcc cgaatattaa aactagaaaa agggcattaa aaattatcaa agaacataaa
    12061 cgaaataaaa tgaagaaatt agcataatta aggaaggaaa aagaatgaag aaaatcagtg
    12121 taggacttat tgggatagca gctctaggat tattgggagc ttgctccagc actaatgatg
    12181 caaaagtatc gaatgataaa gatggaaaat tagaaattgt aacaacattc tatccaatgt
    12241 atgatttcac caaaaacatc gtcggcgatg aagcgaacgt ggatttaatg gttccagcgg
    12301 gatcagaacc tcatgactat gagccctcag ctaaggatat ggcaaaagct catgatgctg
    12361 atgtttttgt atatcataac gaaaacatgg aatcatgggt accaaaagct aaagaaagtt
    12421 ggaagaaagc tgggcctaat gtagtagaag gaacaaaaga catgatttta ttgccaggta
    12481 gtgaagaaga agatcatgat cacggagaag aagatcacca tcatgaacta gatcctcata
    12541 cgtgggtttc acccaaaatg gcaattaagg aagtttcaaa tatcaaggat cagttagtta
    12601 aattgtatcc gaaaaaagca aaagtatttg agacaaatgc tgaaaagtat ttgactaaac
    12661 tgaaacggtt ggatgctgac tataccacta gtcttaaaga agcgaaacaa aaaagttttg
    12721 tgacgcaaca cgcagccttt ggatatttag ctttggatta tggattgaaa caagttccta
    12781 ttgctggact aagtcctgaa gaagagccat cttctggtcg attagctgaa ttaaaagagt
    12841 atgttaaaaa aaataagatc aattatattt actttgaaaa gaatgctaat gataaaattg
    12901 caaaaacttt ggccaacgaa gctgggataa aattggaagt actgaatcct cttgaaagtc
    12961 ttacaaaaga acaaatggat aatggtgaag attatgtttc agtaatggaa gacaatctaa
    13021 aagcgttaga aaaaacaact atggttgctg gtgaagaggt agttccagaa aaagaagcga
    13081 aggatgaaaa gacagttgct aatgggtact ttaaagatgc cgatgtgaag gatcgcgaat
    13141 tgagtgatta tacaggcgaa tggcaatctg tttatccgtt gttaaaagat ggaattttgg
    13201 atgaagtttt cgattacaaa gcaaaaatca ataaagacat gacagctgcc gagtacaaag
    13261 attattatac tactggctat aagacagata ttgatactat caacattaaa gacaacacca
    13321 ttgattttgt agtcaacggt gaacatcatc aatacacata caaatatgtt ggatacaaaa
    13381 ttttgaatta tgaaaaagga aaccgtggtg ttcgttttaa ttttgaaact gatgatgcag
    13441 gtgctggtcg ttttaaatat attcaattta gtgatcatgg aattgcacca agtaaggcag
    13501 agcatttcca tattttcttt ggtggagaaa gtcaagaaaa actctataat gaattgcata
    13561 attggcctac tttctatcca gcttcattgt ctaaacatga aattgctcaa gaaatgatgg
    13621 ctcattaaaa ttcaattttt ggaggaaata catggtaaga aaagtattat tgtctatgga
    13681 ttccaagaat attgatgctt atcggcgtta tcgatttgca aagcaaatga gattaaatgg
    13741 gagtacatta gctgatattg ctgcaacttt acatgttagt attgacacag ttcatagagt
    13801 attaaaacgg gatcctgatt gttgttttat caaggtcaat actggaagaa gtagacgttt
    13861 agcaaaacgg cataatagct tggccaatta attgataata ccagagtaga tcatttttcc
    13921 cttatttgga tttgatctgc tttttattat gtcttgaatt gtcaaatcaa gcgcaacact
    13981 ttatcaaaga agacttcata aggtgttctc caatctaaac actttctagg tctgttattc
    14041 agtttttcaa tcatctgatt tatcgttgag ttatctaccg tagctatctc tttcgatttt
    14101 ggcaagtatt ctcgaatgag tctattcgta ttttaatttg ttcattgttg ccaagggcat
    14161 gaggatctgg aaaataaaag ggcatattat tcaatgcttc tgtaactgcg aaatgattgg
    14221 agaattcttt tcctctatct ggagtgatcg ttttgaccgc ctcatttgga atactgctta
    14281 aaagttcaat cattttctcg ctcacataag cggctttctt tctggggacc ttctttcctg
    14341 ctaaaaggaa gcatgatttt ctgtctgtca aagtcactaa acaagtacca ccagttttgc
    14401 cagctacaat atcggctttc caatgaccaa aatcacttct atcatctgcg gattttggac
    14461 ggccatggat cgaatgagta aggcaaatct ttccacgaga ctcgacatga ttttttgtat
    14521 gtcttgtttt tcctcgatgg tgcaatgaac ggatcaggcc tttattgccg tgtgagaggg
    14581 gttctgtatc gaaaagatca ttgcaaattg cacgttagat cgtgataaaa ctaattgaaa
    14641 agtccatatg ttcatgcttc aaacgattcg agatttcctc tggcgaccac tgaaaatcga
    14701 caaataaatg acgaacaatt gaccaaactc acggatcatt tagaatgtga taacgtccgc
    14761 atttttttct tcgataagca taattttttt ccgctttgga aggtgaatag tttttttctt
    14821 gtttcttctc agttcatgta aaatagtaaa cgggctatgc tgaatgtcgt tgccaattat
    14881 actaatagaa aatccttctt cgtgaagcag aaagattctt tctcgttcga gtatagtaag
    14941 atgacgatac tggctcataa ttattttcct tctaggagtg ttctcgacaa acttatacta
    15001 gttggatatc gattatgact catttttatg tgttgcactt aaagtgtaaa ttcaaggtat
    15061 agaaagaggt tccctatgaa aaaaatacct attacaatct taactggcta tcttggttca
    15121 ggaaaaacga tacttctaaa tcatattctg tacggtgatc acggaaagga aattgctgtc
    15181 attgtaaatg acatagggga agtaaatgtt gatggggtat tagtcgacaa gaaaggtttt
    15241 aaaagaactg gagaaaaact tgtttcgatg ccgaatggat gcatttgttg tacattacgc
    15301 gaagatttga ttgttgaact agatcaatta gtgcaatttt ctgacttgaa tcaaattgtt
    15361 attgaagctt caggaatcag caaaccaatt ccaattattc aagcaattat attagctaaa
    15421 aaccctttag acattgattt gacccaacga ttagagattg gtgccattat tatagtagtg
    15481 gatgtaaaac gtttttggga taatttcgaa acgggtgaaa agattcagga aagaaaagct
    15541 gaaggtaatg tagttgaaga agaacaggat attcgtggct tgttaattga tcaattcgag
    15601 tgttgtaaca tattactttt aaataatagt gacttagtgt caaccaagac gatttacaaa
    15661 attaacggtc taattgctaa gttacagtcc aatgccaaaa taattaggac aattaaaggt
    15721 gaagtagact tatcgaaaat tctaaataca aatcttttta atatagatga gacttcctat
    15781 tcagtgggct ggttcaaaga gttagagctt ggctatcaaa atcatattcc tgaaacagaa
    15841 gaatatggta tctcaagttt tatctatcga aatcggttac tccttgacgc acaaaaattt
    15901 gcccatctta cggataaaaa ttttcctggc aatattacaa gagcaaaagg gtttctttgg
    15961 tttgcccaac atagtgattt ttcgacgatc tttgaaacat cgggtagcta taaaagtatt
    16021 gaatcattaa attattgggt agctagttta cctaaaattg agtgtcaaat gattatacaa
    16081 aaaaatcccg aattgaagga agcctggcat ccgttgtttg gtgaacgcat taataaacta
    16141 gtttttattg gtatgaagat ggatcaacaa tgtattaagg aacaacttga gggttgttta
    16201 actgattctc ttaatgcaga taatttccga aaaatttcac catttccatg gaaaaagtct
    16261 aatatgttta gccagatatc gttaatgaaa tttttaagaa aagctggtgt caagataggc
    16321 gatgtcataa ttattggcat tttgattatt acctctttta ttcccttatt tgtattctct
    16381 atagaaaagg agaatgaaac aactgaagat gctatcaaat atgccatcgt tcgaattgat
    16441 ggcaaggaaa tggatcgctt cgatttggat aagatcaatc ataaactcgt gaattatcat
    16501 cctgcgaagg gacaatataa tattgttgag attaaagatg gaagaattcg agttaaagaa
    16561 gataatagtc ctgatcaaat cgcggttaga actggatgga tctcaaaacc cggtcaaacg
    16621 agtatttgtt taccccataa gttagttatt agtattgaga aaaaagaaag cacagattac
    16681 tacatttact aatctggaaa tagatgagga ggtttttaat aagaaaaaga tattattatt
    16741 aataatagca ggggctttat taagtgtgtt aagcgcttgt ggacaagaaa aagaagcggt
    16801 gacaacaggc acaagtatct ttaacgggac ctgcaatggt ttagttaaca gtcggttaga
    16861 ttacttattt tggctcttcg tcaatgagtg ttggtgaaga aattcaatag aataatcgag
    16921 atactcacac ggctagacag ctatcttgtc tagccgtgtt gttgttctgg ctttagtcag
    16981 ttgggtagta ttcttagttt tcttaaaata ttttcctcga tataaaaaga ttcgttctcg
    17041 gtagttttga aagtttctat agccataggc aattcgtttt agtactttga tatggttatt
    17101 caaacattct atagggccgt tggaataggg aacgcgcaac gctcgtttga tttctgtttg
    17161 gtatttcttg aaggtcgtga acactggttg gtagtgctct ggcaactcct tgtagtcttc
    17221 ttttaggagc gcgacgaatt caggaacatc ttgtcttctg ataacggaaa gaaaatcctg
    17281 gtagacttcg tagcctcgct ttaaggaatc atcataagat aatagccgat cgactatctc
    17341 tgcttctgtc agatgatctc gaaatgacgg acgccagtag cgatgctgac ctttcaattc
    17401 ccacgcattc ttttgaagta gtttccagta tttctttaag tgcttcgacc gacgatcccc
    17461 ttttttaaag gtcttcatta cttgaattcg gtggttggta aaagcccgac caatgtgttg
    17521 gctaatatga aagcgatcgg cgacgattcg ggcattcgga aaacactctt tcaccaatgt
    17581 gcgatagggt gtatagaaat ctgaaaccac tagacgcact tgttcacgga cttttatagg
    17641 gtagcgcaag aaatagtcgc gtaaaggatt gagttgccga ttttccacaa tatcaatcag
    17701 ttgatgggtc ttgccgtcca tcatgacaaa gctcatggct cccgagacat ttttcactga
    17761 tttgaattca tcgaaacaca ccacttctgg taacagctgg ctcaaagaaa tggttttagg
    17821 ccgaaggctg cgaagtattc gaatgacagt ggtcggagaa atgtgcttca tacgtgcgat
    17881 caaagacata gaaagaggtt cgcttaatag ctcaagaatg gcttgtttga ctctgcgagc
    17941 aatggagcag cgacgatcac taactgaatc ttctgctaaa aaagtctttc cacagtgttt
    18001 acatttgaag cgctgtttgt taatccgcag aatggtcttg tagttactga catcattcaa
    18061 taagatggat acttttttcc agccctattt aatgatcgtt tgttcatttt gatagtggcg
    18121 atgaaagcat tcctccggtt gataggtcaa tgtgccatca aaaacaaaac atgtttcgtc
    18181 attgattcgc tctttgcgaa aggcatcttc atgaaagata agatttaggt ctagaatatc
    18241 tagtaactct ttggtaaact ctgaatagga catcttgaaa cactcctttg aatggttttc
    18301 gtcgacttta ttctaaagga ttttaaggtg tctgtttgcg tttattagaa aaaaga
//
LOCUS       DACEUZ010000046        18339 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.046, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000046 DACEUZ010000000
VERSION     DACEUZ010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18339
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.046"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(131..697)
                     /locus_tag="IXG44_001360"
     CDS             complement(131..697)
                     /locus_tag="IXG44_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAQ1658089.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(697..1653)
                     /locus_tag="IXG44_001361"
     CDS             complement(697..1653)
                     /locus_tag="IXG44_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HAQ1658090.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(1653..2309)
                     /locus_tag="IXG44_001362"
     CDS             complement(1653..2309)
                     /locus_tag="IXG44_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAQ1658091.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            2679..4697
                     /locus_tag="IXG44_001363"
     CDS             2679..4697
                     /locus_tag="IXG44_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ1658092.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     gene            5124..7538
                     /locus_tag="IXG44_001364"
     CDS             5124..7538
                     /locus_tag="IXG44_001364"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016183651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HAQ1658093.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            7886..9439
                     /locus_tag="IXG44_001365"
     CDS             7886..9439
                     /locus_tag="IXG44_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAQ1658094.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(9820..11604)
                     /locus_tag="IXG44_001366"
     CDS             complement(9820..11604)
                     /locus_tag="IXG44_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1658095.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNFAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(11619..13340)
                     /locus_tag="IXG44_001367"
     CDS             complement(11619..13340)
                     /locus_tag="IXG44_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1658096.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLQIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            13440..14201
                     /locus_tag="IXG44_001368"
                     /pseudo
     CDS             13440..14201
                     /locus_tag="IXG44_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
     gene            15168..15436
                     /locus_tag="IXG44_001369"
                     /pseudo
     CDS             15168..15436
                     /locus_tag="IXG44_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
     gene            16108..>18339
                     /locus_tag="IXG44_001370"
     CDS             16108..>18339
                     /locus_tag="IXG44_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1658097.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
BASE COUNT         6019 a         3269 c         3712 g         5339 t
ORIGIN      
        1 ttggttggac ttattaccgc agatataagc ttttgagcac cgtgaataaa gaggttatga
       61 aatcaacgct ctaacctgag tattagagaa aaaaacgcaa aacatatcat ttatgttttg
      121 cgttttttat ttatcttttt ttctcaatac aatataaaat aggcggctgg ttcttttggt
      181 tgataaaccg ataattcagc acattatatg tttcctgtgg cagtttggaa caaaaagaat
      241 caaccatatc cagttctttt tctccacctt catgaccata gtagacgaca aggatcaatc
      301 gtccgcgagg cacaagtcgt ttcaatattt cttccatagc tgtgcgtgta gtctctggca
      361 tagtgatgat tgctttatca ctttttggga gataccctag attgaaaatc cctgctttga
      421 gattttgttg ttcagctatc acttctccta acgtttcgtg gccttgtaga aacaatgaga
      481 cacgttcttc taactgacgc tgttctagcc tttcacgagt agcatcgatt gcttgttgct
      541 ggatatcaaa actatagaca tgaccagttt ttccgatgtg ttctgctaga aaaaccgtat
      601 cgtggccatt gcccatcgtt gcatctacga cgtgatcccc tggttgcaag atttcttgca
      661 gtaatgtatg gctgaaatgc aaagcagttt gcaacatcag acgctcacct ttttccgtac
      721 atcatatttt ccttggaagc tatctctgcg aagaagttct tcatcaattg cattcaagac
      781 ttcccatttt ttcaagctcc acattggtcc aatcaaggaa tcccacggcg catcccctgt
      841 caaacgatgg atgatgatct cttttggtat catttccagc tggtcgcaaa tgacattgac
      901 atagtcagga cggctcatta attgaaggcg accttcatga tagtcccgca acattctcgt
      961 tttgcgcatc agatgcatca aatgaagctt gattccttgg atatctgaat ctaaaatggt
     1021 gcgtctgaca ttttctttca tcatttctaa actttcgcct ggtaatccgt tgatcaaatg
     1081 cgtacaaaca cggatattat gtttgcgtaa tttagccacg ccatctaaat aagtttgata
     1141 atcatgtgct cggttgatca gttttgaagt ttcttcaaaa gttgtttgca atccaagttc
     1201 tacccataaa tacatccgct cgttcagttc agcaaggtaa tcaactactt catctggcag
     1261 acaatccggt cgtgtaccga ctgataatcc tacgacacct ggtaaattga ctacttgctc
     1321 aaatcgttct ttgattattt caactggcgc atgggtgttt gtaaaatttt ggaaataaac
     1381 gatgtattgt tggacatgag gccatttttg atgcatcatg tcgatttctt tctggaattg
     1441 gattggaaga gggtcttcag gtgcaacgat catgtctccc gaaccagata cactgcaaaa
     1501 agtacaaccg ccatgggcaa cagtcccatc tcgattcgga cagtcaaatc ctccatcgat
     1561 cggcaccttg aaaattttcc cgccaaactc atttcgtaaa gcgtaattcc aagaatgata
     1621 tcttttattt ccttctgaat aaggaaaagt cattaaaagt ccttctttct gtaagagtta
     1681 tctatttgtt ttcttttttc tctatacact ggataagtca ggcacagcca agaaagactg
     1741 agtaagtagc cgccgatgat atcagtagga aaatgaacgc ccagataaat gcggcttatc
     1801 ccgacactta ctataataaa catcatcagc aattgaagca atgtgcgtaa ggttcttttt
     1861 tgaacaaaag ctggaagcag aaatagtaaa gaaccgtaaa gaatcatact gcctgctgca
     1921 tgtccactag ggaaactgta acttgtttca actactaagt gcgccaatga aggacgctct
     1981 cgcatgaaaa atagtttgat caccgggttg atgattccag caattacaat aaatccagaa
     2041 acgaaccaaa atgtttctgc cctttttttc ttataccaca tccataaagc cgcacaagca
     2101 aataaaatga tgacggtaag aggattaccg aatttagtca taaagagttg gtagctgttc
     2161 agatcaggat aagaagctct gacaatgcct gtaaaccact gatcgaaacc agaaagccac
     2221 gacagatgga actttacgat ccagcccaaa atgaaaaaca ggacaagaaa gaagctacca
     2281 gccacctgaa atgatgtttt atatttcata caattcctcc tttgaagcaa tgacttatta
     2341 tagcattttt ggtccagcga aaaaaacggt aaagagataa aaccaatgta tgattcctct
     2401 ttgaataacc aatattctta taaaaaaaat aactgtaatc ggaaagacat tttttggtaa
     2461 tgcaaatgaa atgttaggga aataaaatca gtgtttttcg aaatcctttt aaacaagaac
     2521 gtagacgtag gaagaacttg aaaatgaaca gaagttgttc attacggtaa attacaatat
     2581 gattacaaaa aacaacaaag ctcttaaata acttttaata aatattaaga gaagttataa
     2641 ttattaataa tagataaatt gaagttggag gatagaagat ggaaaacatt gcaagaaaag
     2701 aacggagacg tttagaacag accaaacgtt ttcgaaaagc aaaacgaggg gcagcaatcg
     2761 tgggaactgc gatggtaggc tgttctgtcg ctgccccatt agtacagccg gttcctgttc
     2821 aagcggatga aacacctgtt cagttcggcg caagaatcaa tacagcggca tttatcacag
     2881 aaattgctac ttatgctcaa ccaatcgctc aagccaacga tctatatgca tcagtgatga
     2941 tcgcacaagc agttgtagaa agcggttggg gcagtagtgc gttatctcaa gcaccttatt
     3001 ataatctttt tgggataaaa ggcagctatc aaggacaaac cgtctacatg gatacgcttg
     3061 aatttttaaa taatcaatgg gtaaataaaa aggagccttt ccgacaatat ccttcttttg
     3121 ctgaatcttt caatgacaat gcctatgtac ttagaaatac ttcatttggc aacggttatt
     3181 actatgccgg tacttggaag agcaacacga aatcctatac agatgcgaca gcttgtttga
     3241 cagggcgtta tgcgactgat cctggttacg caggcaaatt aaataatatc atcgcaactt
     3301 acggattgac ccaatatgat acaccagcta gcggaaacgc aggcggtgga gcgactacag
     3361 gtggcaataa tactggaagt gcaggtagta ctggcggttc aagcaattct ggtaatacaa
     3421 actcaggttc agcaacttcg accacttata ctgtccaatc tggtgattct gtttggggaa
     3481 tctcaaataa attcggtatc acaatggatc aattgatcca atggaacaac atccaaaaca
     3541 atttcattta tccaggacaa aaactgacaa tcaaaggtag ccaggaaaat ggctcttcga
     3601 cgaataattc cggtaacaat accaactctt caggaaatgc tggtacatca aacggaggac
     3661 aaacaactgg tgcgaaatac actgttcaat ctggtgattc tgtttggaag atctcaaatg
     3721 atcacggaat tacgatggct caattgattg aatggaacaa tatcaagaac aatttcgttt
     3781 atccaggaca acagctaatc gtatcaaatg gcggttcttc taatgctgga gcaaatactg
     3841 gctcaaatac tgggaataca agtggctcta gttcgtccag ctcaagttct tctaatacag
     3901 cttcaggaac gaaatataca gttaaagccg gagactctgt ttggagcgtc tcaaataaat
     3961 acggaattac gatggctcag ctaatccaat ggaacaatat ccaaaataac tttatctacc
     4021 caggacaaca actgactgtt tcaaacagtg gatcaacaaa cagcacgtca ggtaatacag
     4081 gttcgtcaac caatacaaat aacagcagtt cagttaatac tggcagcagt tcgtctcaag
     4141 gcacttatac cgtgaaagca ggtgaatctg tttggagcgt ggcaaataaa aacggcatca
     4201 cgatggatca gttaatcgaa tggaataata tcaaaaacaa cttcatttat ccaggacagc
     4261 aattgatcgt taaaggtgga acttcggcaa atacaaatac aggttcaact acttctgcag
     4321 cgaaaccaaa tacaccgaat acgtctgcta cgacaagtac atcatccact ggtaatacga
     4381 tgtataccgt gaaagcgggc gagtcagttt ggagcgtagc gaacaaacat catatcacga
     4441 tggatcagtt aatcgaatgg aataatatca aaaataattt catctatcca ggacagcaag
     4501 tgattgtgaa gaaaggttca tctcaatcaa caaaccaaca atcagttact ggttcgaaaa
     4561 cctatactgt gaaatctgga gaatctgttt ggagtgttgc tgacagtcat ggaattacaa
     4621 tggctcaact aatcgaatgg aacaatatta agaacaactt tatttatcca ggtcaaacgt
     4681 tgattgtagc taaataaaat agctgttcga agaaataacg attgaattcc acaaaaatat
     4741 ttgttactat aaggctactt aaatatgaca acacttgaaa aggaagtagt aaaaagactg
     4801 aagtttcaga gagcgcatgg ctggtgagaa tgtgcacgga tgcttttgaa ctcgcctttg
     4861 agttgccctt gctgaatacc cgagtaagca agcggcggtg acggccgtta caacgttcga
     4921 ggccttttca ctaaggtaaa attaggtggt accgcgtaga ttacgtccta taaaaagagc
     4981 aatcttttta taggattttt ttatttcttt tgtgaagttt gagaaaacaa agcaaaagga
     5041 aataaaaata aacagattct gccggttttt tcgttatatt tataatagaa acaagtgttt
     5101 tcaatagaac aggaggacag caaatgagtt acaatcataa agaaattgag aaaaaatggc
     5161 aaaaatactg ggcaaaaacg aatactttca acacccatga tgatcctgaa aagccaaaat
     5221 tttacgcact ggatatgttt ccctatccat ccggtcaagg gctacatgta gggcatcctg
     5281 aaggttatac ggcaacggat atcctttcac gtgtgaaaag aagtcaagga tacaacgtgc
     5341 tgcatccaat ggggtgggat gcattcggtc tgcctgctga acaatatgcg ctagatacag
     5401 gaaacgatcc agctgagttt acccaaaaaa atatcgaaac attccgacgc caaatcaatt
     5461 ctttaggatt tagttatgac tggaatcgcg aagtgaatac aactgatccg gagtattata
     5521 aatggacaca atggatcttt acgaagcttt atgaaaaagg tctagcttat gaagcagaag
     5581 tagcggtaaa ctgggtacct gaattaggaa cagttatttc aaatgaagaa gtgatcgatg
     5641 ggaaaagtga acgtggtggc tatgatgtag tcagaaaacc aatgcgccaa tggatgctga
     5701 aaatcactgc ttatgctgat cgcttattgg atgatctaga attagttgat tggccagaaa
     5761 gcattaaaga aatgcaaaga aattggatcg gtcgttcagt aggagcaaat attgaattta
     5821 aagtggctgg tacggataaa tcctataccg tatttacgac acgaccagat actctattcg
     5881 gtgcaactta ctcggttctt gcacctgaac tagatcttgt tagagaaatt acgacccctg
     5941 aacaaaaagc tgctgtagaa gcttatatcg aagagacagc taaaaaatca gatctaaaga
     6001 gaacggatct tgcaaaagaa aaaacaggag tatttactgg tgcttatgca atcaatccag
     6061 ttaatggcaa ggaaatccca atctggatcg ccgattatgt attatcttca tacggcacag
     6121 gagccatcat ggctgttcca gcacatgatg aacgtgactt tgaattcgct caaactttcg
     6181 gactagaaat tcttccagtt atcgaaggtg gagatgttga aaaagctgct tacacagaag
     6241 atggtacgca catcaactct gaatttctag acggcatgaa caaacaagaa gcaatcgata
     6301 aaatgaatga atggctagaa gaaaatggcg tagggaaaaa agaagtcagc tatcgcttac
     6361 gtgactggct attctcccgt caacgttact ggggtgaacc aatcccaatc atccattggg
     6421 aggacggaac agtcacagct gtcccagaag aagagctacc attacggttg cctaagacaa
     6481 aaaacatcaa gccaagcggt actggagaat ctccattagc aaatatcgag gagtgggtca
     6541 atgtcgttga tcctgtaaca ggtaaaaaag gccgtcgcga aacgaataca atgccacaat
     6601 gggcaggaag ctcatggtat tacctgcgtt atatcgaccc tcataacaaa aaagaactag
     6661 cagattatga aaagttgaag cgttggctac cagttgacat ctacattggt ggagcagaac
     6721 atgcggtact tcatttacta tatgcacgat tctggcataa attcttatac gatatcggtg
     6781 ttgttcctac aaaagaacca ttccaaaaac tatacaatca aggaatgatc ttaggcgaaa
     6841 acaacgaaaa aatgtcaaaa tctcgtggta atgtagtcaa tcctgatgat gtggtcgaaa
     6901 cttacggtgc agatacactg cgcatgtacg aaatgttcat ggggccatta gatgcttcga
     6961 ttgcttggag cgaaaatggc ttagaaggaa gccgtaaatt cttggatcgt gtatggcgtt
     7021 tgatcgttga tgagaacaat aaaatgcgtg accgtatcac tacattgaat gatggcaagc
     7081 ttgataaagt gtatcatcaa acagtgaaaa aagtgacaga agactatgaa aatcttcatt
     7141 tcaatacagc aatctcccaa ttgatggtct ttatcaatga agcctataaa gtggacgctc
     7201 tgccttatga atatatcgaa ggatttgttc aactactcgc accgattgct cctcacatgg
     7261 gtgaagaact atgggcaatc cttggaaatg atggtggtat ttcttacgca ccatggccaa
     7321 catacgatga agcagcactt gttgaagacg aagtagaagt tgttttccaa gtaaacggta
     7381 aagttcgtgc gaaaagcaat gttccccgag acttaggaaa agatgagcta gaaaaagcag
     7441 cattagccaa tgaaatagtg caagaatata ttgaaggcaa aacagtcaga aaagttattg
     7501 cggtaccaaa caagttagtc aatatcgttg ctaattaatt ggctctttgt caataaggac
     7561 tgatgagttg tgcaaaatca aatctgggcc agaatgaact tcattctgtc tcagattttt
     7621 tgttatttca aattgattaa ttgattgatc aaaaaggttt tgattttcat gttctcaaac
     7681 acgttcaatg tctttttctt ctgaatggtc ttgtaagaga tctctgtaat catcaaattt
     7741 ttgtctatta atcttagtat tttttgatgt ataaactacc tgaaattgag agtacatacc
     7801 aaattcataa attttttatt agtgggacta gtgattgtat ggattttgac gcataatgaa
     7861 tgaagtatga gaaaaggaga gacaaatgga tcaagaacta tttattatcg aagtattatc
     7921 agctaaacaa aaaattatcg agaaagcgaa aaaaagagcg aaaagaataa tgttcaagct
     7981 tttaagctta gtatttttct ctggtttgct ttttttcgga gctgtatcgt tcctatatcg
     8041 ccatatgagt gcagtagtcc gttcagctga agggatgcaa aaattaaata atttgtatca
     8101 cttttcgtgg ggagttggcg gaataacaat tgttgtgctt attgtttttc ttctacttat
     8161 ttggctccgt taccatcaat tgtcgcgtca tttcgatcaa tggcttagtc aaagagcaca
     8221 acagttttta cataataaac caacttctca agatgatgac tatctttatt ttgagaagac
     8281 ttcgacgagt caagcttttc ggatacaaaa aagtgattgc cagttattgc tgagatcact
     8341 tgatgattta cctatctact taggcgaaag caaatcaaaa ttcggaatga acacattcca
     8401 tattttttta ctagaaaacg aacccagatg ggaaaaaagc cagtcttcgc ctggtggata
     8461 cctgacaact aagatgagta cattagtcac tggttttttc ctcattcttt tagctggaac
     8521 aatctattat gggagataca ctcagccaaa aatgtcttct catataaatg ctgcttctca
     8581 catgacaatg aactcaacta acgatacgca tatacaaaag acaaatgaag ggttgttagc
     8641 tgatcctaag caaaaggtaa atttgacaac acaaaagata aatgatctca aacttgatac
     8701 agagacgcac tctctctata tgacaacaga tagtggtgct aattgggtat ttgtgccgat
     8761 tgattcgcaa tggttgcgtt ttggcgaata caccctgaca acggggctga ttcctgaagg
     8821 ttattggatg gacaacacat atgatatttc cgccgatttc tcatggttta tctattcttc
     8881 tggggatgat gtacgtctgc tgacttcaag agataatggt aaaacatggc aaacgtctat
     8941 tatcacccaa tcagttcagc cgatacgtta tcgaaaagcg gtattttatg gcaacgatgg
     9001 tctatttatc tgtacatcag ccagtgggat gtctgcagaa actttgtaca tttacactac
     9061 tatggatggc ggaatcactt ggataaaaag tggaagtaca atgatcaatc agccagtaca
     9121 aaatgtcagc ttcgttacaa cttctttagg ttttgtttcg acacgtgaaa gtttgtttta
     9181 tacaaacaac ggaggacgct cattcaaaga atcagttctg tcgattccgg aaaactatca
     9241 gacagggggg atagatcttt ttcaatcacc aaatgaagtt actcaaataa gcgcgaatac
     9301 gttagagaca aaattttatt tgttgaaaaa aggaccaatc gatgcaggga aaatgtttgc
     9361 ctgcctcttc cgttcaactg ataacgggga aacatggcaa ttcgttcagc aactatcgca
     9421 agtagaacaa ggagattaaa gaagagagat tgtgagcctg acgttcagca ccgagtcaca
     9481 agaaacaaat tcaaaaaaca gcttctcata tttgttgaat agtgtaactc gtgattcgga
     9541 gttgacaaga gcaacaagaa gcgattttga agtatagctt ctcgtatttt acaagatcgt
     9601 gtcttattgc cgataggttg tctcttgaac accgtttatt cgtaaaaagg gattgtgaca
     9661 agtcttttgt cactacttac cgaacgaaac tgaaggagct atccaaaaag tatcattagt
     9721 attattcttt gtgtttctag tggcaccact agtacattta aaaaagctat cgatcatgga
     9781 ctaatcatac atgatcgata gctttttaga taatgtttgt tacgctatct gaaattgact
     9841 attataaaga ctggcataaa gcgttggtga ttggagtagt tcagaatgtg ttcctttttc
     9901 gacgatatcg ccattcttca ttacaaggat catgtctgca ttttcaatcg ttgacaaacg
     9961 gtgtgcaatc acaaaacttg tcctatcttt tgtcacagct tccattgctt gctgaatcaa
    10021 tgcttctgtt cgtgtatcta cgctagaagt tgcttcatct aaaataacga caggaggatt
    10081 cgctagaatg actcgagcaa ttgtcaataa ttgttgctgt ccttgagata acgtgccgtt
    10141 ttcgctagaa atgatcgtat catatccgtc tggcagtgtt cgaataaagt gatcacattg
    10201 tgcgatttca gccgcttcga taatttgttc tcttgtggca tccattttcc cgtaggcgat
    10261 attatcagct accgttcctt caaacagcca ggtattttgt aaaaccatcc caaaagaacg
    10321 acgtagttct ccacgtgaaa gcttggtaat atcaagaccg tcgaattcga tctttccttt
    10381 attcagttca taaaaacgca tcagcagatt gactaaagtc gtttttcctg caccagttgg
    10441 accaacgatc gctaccgttt gttttggctt tactgaaaaa tcgacttgat tcatcaatat
    10501 tttatctgat gtgtaaccaa actgtacatt tttgaaagct atttttcctt tcggttgttc
    10561 taaaagtctt gcatctggaa ggtctggaat ttcatctgtt tcatctaata tctcaaatat
    10621 cctatcgatt gctgccattg cggattggat cgagttgatc acataagaag cggtagaaat
    10681 tggttcagaa atctgattga tgtactgaag ataagcttgt aagaagccga tcgtcacacc
    10741 acctgataga acaagcatag caccgaatac cgcacttatg atgaaggcga gttgattaat
    10801 gaaacgaatg gccggataga tagcaaagtt cataaattga gcacgtttaa aagcgttgta
    10861 ttgcttttta tttacttcgt gtatagcagc ttctgcatga gcttgctgat tgaatgtttt
    10921 gaccactaga ttaccagcta agatttcttc cattcgatta tttaaaacac ccagttcata
    10981 ctgattctgt tcagaaatct gtttgttttt gttcgcaatc ttagttgtaa caattgaact
    11041 gccaacgatc agcaataaga cgagcaaagt aagcttgctt tcgatataaa acagcatcag
    11101 accagcagca gctattgtta cgacagaagt aaaaaattga ttgatacctg tcaataacac
    11161 ttgagaaagt tgattgagac cggtagttgt ccgactaatg atgtcaccta cttgatgatt
    11221 gtcaaaaaac gacatcggta atgttttgaa tttttttgtg atttcttttc gcaccctcaa
    11281 tgtgatcttt tcgcttaacg ctgccattgt ccgttcctga ataaatgaag tgatgctgct
    11341 taagactgaa cagccgatca gcaataagac agggaacaac aatgcttgtt tgatatcact
    11401 gaaagaaagc tgtccaatac ctgtttttct tatttctgat aagagatcat cgatcccgat
    11461 tcccatgatc aagggcatgg ctacgactaa taggttcccg aataaactgc atagaaacaa
    11521 tccgtaaaaa tatttatgtt caggtttgat cattttccaa aaacgtatca aagcacgcgt
    11581 gttcaacgtt tttttctttt ccatcgtatc ccttctttct atattccttg tgattttgca
    11641 aactcctgat aagtttggca attagacagt agttcttcat gagtaccttg tccaacgatt
    11701 tttccttcat ctaaaacaat gatgttatct gcttgttgga ttgtactgag gcgttgagca
    11761 acaatcacga gagttttctc cgacatgtgt ttatgaaggg ctgctcttaa tgctgcatct
    11821 gttttatagt ctaaagcgga gaaactatca tcgaatacat agatctctgc tggtttgatc
    11881 aacgctcgag caatacatat ccgctgtttc tggccccctg aataattaga accgccttga
    11941 gcaacaaagc tgtccaatcc gtcaggaagg gtgtccacta gttcttctaa ttgagcgatc
    12001 ttgatcgctt tagacaactc ttctgttgag gcatattcat ttcccatctg caagttgctt
    12061 aaaattgtcc cgctgaataa gaaagccttc tgagggacat aactgatgtg ggagcggatc
    12121 tgttcttgag tcatttgagt aagaggaata ccaccaaggg ttacttttcc tatcgtaggg
    12181 tcactaagtc tcaagagaag tttcgccaaa ctactttttc cagatccggt cccgccaacg
    12241 atcgctgttg tttttccttt aggtattgtg aaatcaatat cttctagaac tggatcagag
    12301 atttcagtat atttaaatgt gacatgttca aaagcagctg catttgtctg atcaaaggtt
    12361 ggttcagcta ctgttttgat cgtctcttcc tcaatttcga tctctgcttg cagtatttct
    12421 tctatccgtc gtaatgaggc aaacgattta gggataatga ccaataccat agctgcgatc
    12481 atcaagtaag caagtgtcag catagagtat tcgactacag cagtcacgac accgatttgt
    12541 aatgctcctt ctgctacaag atttccgcca aaccacaaaa tagaagcata aacgattccc
    12601 atgagcaaaa aagcaattgg agtgacccaa gcaaatattc ggttcacttt gatcatttga
    12661 ttagcatagt cagcaaagct cttattcgtt cgcttttctt catatgattg attatcaaag
    12721 gcacggatca tattaatccc agtaaagaat tctctcaatg tgatggtgat ccgatccatt
    12781 tttggctgga tactcaaaga taaaggtgcc cctcgtttca tcagtcgata aacaatccat
    12841 acataaaaaa caacagagat caatgtaacc catgttagta atagagaata acgaaacatc
    12901 atgagaatgg tgaaaacgca aataaccggt gctggtaaga tcatttggca aaataaaaca
    12961 atcatttgct ggacattatc gacatcattc gtcattcttg tcaaaagaga tccagaacta
    13021 aaacgactga tatccttcat tgacagctgt tggaatttac ggaaatattt ctcccgcgtt
    13081 tggaatccaa acctagcgga agccaatgct gaaaagtaac tagaggcaac tgcagcgatt
    13141 gttcctagaa acgctacaag cagcatttca actccaattt tttttataac tgaggcttcg
    13201 ccacttgcta tacctgtgtc gatcagcttt gctactaatt ttggtacacc taatgtaccg
    13261 gcgatttgta aacagatcat cacaaaggta aggagagcga gtcctttgtt tttctgtaag
    13321 aatgttttta agatagacat attattcctc cttttttcct gatacttttc gattctatac
    13381 cttcaagtaa cttgaaggtc aatagaaaaa agaataaact gaaaaagagg tgagaaaaaa
    13441 tgaaaaaaat gtatacgatt ggccaagtat aggaactatt tgatttgcca aaatcaacga
    13501 tccgctattg ggatgaacaa ggattaatcc gttcaagtag acaagaagag aatgattatc
    13561 gattgtttga tatcgatgat atttttatgc tttatgatat cgttttttat agaaaattag
    13621 atataccgat caaacagatg aagaatttat atggaaaaac attgacggag ttatatgaga
    13681 cacttgatga aacagaaaga agaatccata aagaactggt ggtgatgaaa caaaaacaaa
    13741 aagagataag ggagcaaaaa aaacagttga aattgatgat cgatacaaac gaggaagaat
    13801 ttcctgtaga agagatccct tttgactgta tgatctcaac ggagtttgaa gatattgtgg
    13861 aaatcaaaaa atttctacct aactattcaa gctttgggat gatgagtatg ccagcttcca
    13921 gttcacagac gatgtatgga ttttttatcg accctagtga agtacattta tttactcaag
    13981 acgtgatttg ggagaaaaaa gatacggctg tttatcgtcg attcttattg aaatccgaga
    14041 tgaaccatgc ggaacgtaat aatattttag aaatcagaga aagaatgtat gaaaaaggat
    14101 ggaaaacagg agaagtgatc ggccagtatc tattgacgaa tacggatgaa aacaatatca
    14161 gaactgaata ttatcatgca tggatagaaa tgaaaaaata ggaaatatgt gtttaagata
    14221 actcgaaaat gatggttgtt ttagttataa agaaaaattt tctggttcga ttacgggttt
    14281 aatgtggggg attattagtc gtggatagat agaattttag ttttttagag gaaatatgtc
    14341 tttaaaaatg gaatggttgc aattcttagc gatatatacg atgtaattgt cctcttaaaa
    14401 aagtatggaa aaataattgt attgggtgtt atgtataatt catggtgaac tgagactaat
    14461 gagaagaaaa attcatattg ttatgagtga gattgtatca gatgagaatg ataaattaaa
    14521 taggtgcata aaagcattac acttaaaata cattgttgct tacattatta caatatgtta
    14581 ataaggcaaa aataaataga tggattgaat aaacatattt tttaatcaaa tagtccctac
    14641 tgtgactttc ctcagctatc ggtgatacta ccgatagcat ttttttgctt ataaaataat
    14701 tataagtatt tagagatttt tcttctaaca ttcttcaaac aagaaaaatc acaaaggtta
    14761 ttgttatttt tatatattct ttttaagaat gattgttttt ttatagaaat tttaaaaaat
    14821 aaaacacgtt attgatggat agttagtttg ttatttggta atattataga agttgactgg
    14881 tagtgattta tttttcgtcg ttttataaaa atgttatttt ttggaaaaat tcacaaatat
    14941 gtaacgaatg tttttctgga atttgctgtg ttaaaaagca gtatgactaa tataaatact
    15001 attgtcattt atgcaatttt ttatggatag taattttaga ggggaatcaa tgaaggaaga
    15061 atagaaggga aaaatagttg caaatacgcc agttagcaaa aatagcaata catcttcaag
    15121 ctttaaaatt cgagagaatc atcttggtag tggaatcaac tacaacaaaa ggattaaacc
    15181 aattaataga tagaattacc aatggaaaaa tagaaaaggt agttattcta tacaaaagtt
    15241 gtttaattcg cttcggttat gaacttagtg aaaccctttg caatatatgg tacagaatat
    15301 agaaattatt gacattacag aaaatgatga agatttaata cagattgtca ctatttgtag
    15361 atacctttta caaggaaacc gagccaataa agctaagaag atgattaagg agttgatgga
    15421 agacgataca agttaagaaa gtgaaactac gcccaaataa agagcaaaaa gagtaattgg
    15481 acgtggaaaa tgcaagaaat gaaccatcga ttcggtggac agtttattag taatgacggt
    15541 ttgagaacat ccatgacgag aatgaaggaa catcctaaat gcgtttagtt gaaccaagtg
    15601 ccaaataata tggccaaata agcgatcagt tatcgatgaa tcccaagtat tctaaactat
    15661 atattcattt tgatgctaag cattgatata tttttttggg attgatgaag gttaaccaat
    15721 acagggactc actgatgaaa ttctagagtc tgaccttggt atcaacgatt tagtgattgt
    15781 ttctaatgga aaagtataaa agtatataag tacattaaca aaataactat tagggttacg
    15841 ctccagcttg tgcaaggcac tggcaggagg aatcgtgaaa tcataattat ggttaagcca
    15901 cacggacaac taaaagtcat acaaagctat cgaatcaatc aacagaagac aatcggcaat
    15961 cattagcttt gtttacagct tacattgcaa cgcttgagtt gaaccgaaaa tacgagggag
    16021 gtaacgcagc cattttgagt tggggaatct ttttagaaaa ttatcgggta ccttgttgtt
    16081 tagtcaaaat tataaggggg agtaatagtg aggaacaaat ttcataaact gttaagtatg
    16141 tttctagtta tgcttattgt gatcaatcaa tttgggaaca taacggttct agctgagtct
    16201 ttagcagaac agccagagcg ttcgctgacg atcgaagttg atcaagagga ggctgctttg
    16261 ggtgagcagt tccagttgcg agtcagagga acaccagacc tgctaaatgc attggtcatt
    16321 gataagagtg aggatttgat tgaaggggaa gtgaagcaac tcaatgatta cgaacgtctg
    16381 attacttatt cttcccagca ttcaggtaaa tacgaattga ccgcaagaag cggtgatatt
    16441 caatcaaata cggtctcaat agaaatcagt gtaccagaag aaagcacaag tgagtctttt
    16501 aatgtaccag aagaaagcac aagtgaatct tttaatatac cagaagaaag cttaagtgaa
    16561 tcttttaatg aagccagatc atctgagcca tctataacgc aggaatctca gattgaaaga
    16621 caagaggagc tcttgaatta tgacatcggc atctctgctg agcgccccac tttgccgagc
    16681 aggcatacac aaacgttaaa gggatacatt caaggaggcg ctggcgatat tcttgaagaa
    16741 tatggggaga tggtcgtacg agttcctaga gcatcgattg gcttaattga gttcattaga
    16801 gtggaggtcg ctgatgattc acctttaaag ccactggcat tttaccaaga aggcacagac
    16861 gttgtttttc catttacctt tgattatgaa ggattaaatg aagccgctca gtcgtataca
    16921 ttcagtatca cgtttgaagt tgctttcttt caatcaggcg gtactgagcc aaataatgac
    16981 tttcaagtga gtcttactta tgctgagcga gcagcaaaca cggaattttc aattacgcct
    17041 attccttcat caagacccat tttttcgaaa ttatactatg gctctagaga aatcgacagc
    17101 caaggaaatc ttgggaatct gctgattgac gggcaagacg tgaccaacag taataaattt
    17161 attttgcagg taaattatga aggatcacga aaagacggtg tagtggttac cgatgtacta
    17221 ccgcaaggaa caactttggc accgaatccg actacatgga gttccactgg aaatagagaa
    17281 cctatttcag gtggggttcg gatcgcagaa gtaacgaatc caggtgaagc aaacgttaga
    17341 actcattatg tttccgagga atttgtggac aaagtatctt ttaataagga aacgaataca
    17401 cttgttgtgg actttggtaa tctagcagag ggtcaatcgt attttattga atatgctctt
    17461 gaagtgaccg atgtcgactt gttcaatgaa ggaagacaag aggatgggtt ttctcggaat
    17521 cgagcgagta tgacctacaa taatcaggta atgagccgag actaccgctt gagaatccct
    17581 gaaagtaatc ttccggcaat ctctttaaga aaaagtgtgg acaaaaatgt tatcaatctc
    17641 aatgaaaatg aactaacgta tactctgcat gtctcgatgt tttctggaac aagaaatacg
    17701 cttgttattg aagatgtttt ggaagaaggc atggaatttg tgtcatttct atcagatatt
    17761 ccctctttca ttgatgccga agtcaataat ggaatagttc gtttcagtgc gaataatacg
    17821 attggtggtc cgacaagttt taccctctct tttaaggtca atgtagaggc gtatgcggtc
    17881 ggcgatcaaa ttttaaactt tgcaacgctt gtgaatagtg acaatcgatt gaatacgagt
    17941 actgtaagaa caaggaaaat cgatgggcgt attaaagtaa ttaaaactga tgagaatggc
    18001 caaaggctgg caggagcaaa atttgaaatt ttatcaagcg attcacaagt gatacaagaa
    18061 ggtataacgg cagaaaacgg agaattttta agtgaaccgc ttcctgtagg tagctatcaa
    18121 gtccgagaaa cagaagcacc ggaagggtat cttttagatg cgacggtaca tgatgttttt
    18181 ataaatgaat actccataga agaagtgacg ttaaccattg aaaacatcct tgatacgggt
    18241 ggtgttgaac taattaaaca tgctgaaggc agtggtgaag tccttcaagg agctgttttt
    18301 gagttacaga accgagaagg agaaacgcta caaaccggt
//
LOCUS       DACEUZ010000047        17687 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.047, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000047 DACEUZ010000000
VERSION     DACEUZ010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17687
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.047"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(136..1716)
                     /locus_tag="IXG44_001371"
     CDS             complement(136..1716)
                     /locus_tag="IXG44_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HAQ1658098.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(2117..3493)
                     /locus_tag="IXG44_001372"
     CDS             complement(2117..3493)
                     /locus_tag="IXG44_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAQ1658099.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(3621..4739)
                     /locus_tag="IXG44_001373"
     CDS             complement(3621..4739)
                     /locus_tag="IXG44_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ1658100.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            4881..5315
                     /locus_tag="IXG44_001374"
     CDS             4881..5315
                     /locus_tag="IXG44_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1658101.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(5393..5926)
                     /locus_tag="IXG44_001375"
     CDS             complement(5393..5926)
                     /locus_tag="IXG44_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HAQ1658102.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(6020..7198)
                     /gene="mutY"
                     /locus_tag="IXG44_001376"
     CDS             complement(6020..7198)
                     /gene="mutY"
                     /locus_tag="IXG44_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HAQ1658103.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(7191..7988)
                     /gene="recX"
                     /locus_tag="IXG44_001377"
     CDS             complement(7191..7988)
                     /gene="recX"
                     /locus_tag="IXG44_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HAQ1658104.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            8095..9477
                     /gene="rlmD"
                     /locus_tag="IXG44_001378"
     CDS             8095..9477
                     /gene="rlmD"
                     /locus_tag="IXG44_001378"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAQ1658105.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(9651..10229)
                     /locus_tag="IXG44_001379"
     CDS             complement(9651..10229)
                     /locus_tag="IXG44_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin-binding protein"
                     /protein_id="HAQ1658106.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            10551..11021
                     /locus_tag="IXG44_001380"
     CDS             10551..11021
                     /locus_tag="IXG44_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HAQ1658107.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGEYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(11129..12355)
                     /locus_tag="IXG44_001381"
     CDS             complement(11129..12355)
                     /locus_tag="IXG44_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR family transcriptional regulator"
                     /protein_id="HAQ1658108.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(13109..13309)
                     /locus_tag="IXG44_001382"
     CDS             complement(13109..13309)
                     /locus_tag="IXG44_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ1658109.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(13443..14051)
                     /locus_tag="IXG44_001383"
     CDS             complement(13443..14051)
                     /locus_tag="IXG44_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HAQ1658110.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNAAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(14136..14606)
                     /locus_tag="IXG44_001384"
     CDS             complement(14136..14606)
                     /locus_tag="IXG44_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HAQ1658111.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(14654..15568)
                     /locus_tag="IXG44_001385"
     CDS             complement(14654..15568)
                     /locus_tag="IXG44_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAQ1658112.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKNVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            15714..16517
                     /locus_tag="IXG44_001386"
     CDS             15714..16517
                     /locus_tag="IXG44_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HAQ1658113.1"
                     /translation="MTTKQLIISSFIRFKDLKETRNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKELR
                     SFLSKASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            16695..17642
                     /locus_tag="IXG44_001387"
     CDS             16695..17642
                     /locus_tag="IXG44_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAQ1658114.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
BASE COUNT         5363 a         3543 c         2910 g         5871 t
ORIGIN      
        1 gacatttcta tgattttaat atagtaaaaa gagccgtcac ggaaaatccg tcacggctct
       61 aaacaaaata tgagaggctg tttttgtttg gctattctta ttacttgatg cagggcagct
      121 tgtgtcaaga cttctttaca tcaaactttt taattttaca ttgggatatt tatccgcaaa
      181 ccagcgttct gcaaattggt tctcgaataa gaacaacggt tggtcgaaac ggtcacgagc
      241 caagatattg cgacttgaac tcatcttctc atccaaatct tctggatcga tccagcgagc
      301 gattttgtgt cccattggag acatgattac ttcggcgtta tactcattta acatacgata
      361 ttggaatact tcgaattgta gctgaccaac agctccaatg atatattctt ccgttagata
      421 tgttttataa agctgaatcg ctccttcttg aactaattga tagattcctt tgtggaagga
      481 tttttgtttc atgacatttt ttgcagtcac tttcataaac agctctggag taaatgaagg
      541 gagttcttca taagcaactt ttagttttcc ttcgaacaat gtatccccaa tctgataatt
      601 gcctgtatca tatacaccga tgatatcgcc tgctactgct ttttcgacgt tttctctcga
      661 atctgccata aattgtgtga cattgcttaa tttcagtttt ttatttgtac gttccaacca
      721 gacatccatt cctcgttcga atgttccaga acagattcgg acaaaagcga ttcggtcacg
      781 atgggctgga ttcatatttg cttggatctt gaatacgaaa cctgaaaatt cttcctcata
      841 aggacttacc atctgatcat cttgtgtttt gtgtccataa ggagctggtg ccaattcaac
      901 gaaagtctct aaaaaagtct gtacaccgaa gttagtcaat gcagaaccaa aaaatactgg
      961 tgtctgatcg ccacgagcaa ttttttcttg attaaatgca tctccagcct ctttgattag
     1021 ttctacttct tctaagactt gttcatataa ggaattttca gttagaggat gattttcggg
     1081 aagcagtcca tcttcatcca attctactaa tcgttcatcc tgataatttt ccggacgata
     1141 aaactctaca cgattatggt agatatcata aagaccttct agccctttac ccataccgat
     1201 tggccaattc atcggataag attcgatatc taatagctct tctaattctt ctaacaattc
     1261 caatggttca cgaccatcac gatctagttt gttgataaaa gtaaatatcg ggatgccacg
     1321 tttttttaca acttggaata atttctttgt ctgtgcttcg atcccttttg cactatcgat
     1381 taccatgacc gcactatcca cggccatcaa agtacgataa gtatcttcag aaaaatcttc
     1441 atgtcctggt gtatccaaga tatttactcg tttcccttga taatcaaact gcataacaga
     1501 actagtaaca gaaatcccac gttgtttctc gatttccatc cagtctgatt tggcaaaatt
     1561 acctgttttt ttgcctttta cggtcccggc ttgacggata gccccaccaa acaatagtaa
     1621 ttgttctgta atcgttgttt tcccagcatc cggatgggaa atgatcgcaa aagttctgcg
     1681 attgtccact tgttgcttta attttggatt attcataacc taactccatt tcttccattc
     1741 aattatttat attttctcta ggcacagatt gtccgatctc ttaaagaaag acaatttcat
     1801 atagggctgt gacaaaagcc gtgtagcagt ctctttttcc gagtaaacgg cgttctagag
     1861 acagcctatc agcaataagc ccacccaagc caaaaatacg aaaagctgtt tttgcttggc
     1921 tgctcttatt acttaatgca ggatagcttt ttcacaacct ttgtcaacta ctacaactga
     1981 atcagtatag catggattca gcgaatgaaa aagccaccaa accataagag tcaaatattt
     2041 ttttgtcagc tgactaaaag aaaagacgtg ttgcttgttt attcaagcaa cacgtctttc
     2101 ataaccaagt ttccaattat cttcttggct cgtcatcttc gaattctttt ctgaagtatc
     2161 ttcgttcttc ttcaggttcc gcttctgttt cttccggatc atgaaaaatg acacggattt
     2221 taaggattcg agaaccttcc atttcttcag aaaccagcgt aatattatcg acatcaaacg
     2281 ataacttttc gccttcatcc ggaatcatgc ctaatgctgt aatcaaataa ccagccatcg
     2341 tatctacatc actcatatgc agatcactgt caaaagcttc attgaactca tcaatcagca
     2401 tacgtccttg gatgatatat tcgtgttcat cgattttttc ataaagattc tcaacttcat
     2461 ctgattcatc atcgatttct ccgacgattt cttctaaaag gtcttctagt gttgctaacc
     2521 caacgacgcc gccatattca tctaataaaa tagccatctg attctgcgtc tttttcattt
     2581 catataacag atcatcaata aagaccgttt caggaacgaa taaaggctcc tgcatgattt
     2641 tcttgatatc taaattatca aagccgaatt tatgggcagc ttttagtaaa ttctttgtat
     2701 gaaggattcc gactacttta tctttgtctt cgttataaac agggattctt gaatagtttt
     2761 cggataaaac ttcattcaca ttttcttcca cagtgtcgtt gatatcaatc ataaatgcat
     2821 ccgttcgtgg gaccatgact tctcgggcaa ctttcgtatc aagagaaaaa accccttgga
     2881 gcatttctaa ttcttcatta ttcaatacgc cttcgttttc aagcatataa cgcatttcat
     2941 ctctggtcat ttttgaatct tcatcatcaa aagtcatcgg cgtgatacgc gccaataaac
     3001 ttgtggaagc agaaagcagc cagacgaagg gcttagctac tacaccaatc aagcgtatca
     3061 gccctgaagt gaactttgcg acttcttctg atttatttaa agcaatacgt ttagggtaca
     3121 attctccaaa aacaatcgat acataggtca aaatagccag cacaatgata tttgcaatat
     3181 tttttgccgc tgtgccgcca cctaataccg gagctaaccg ttccgataaa gtattagcta
     3241 gtgaagcacc agacaaaatg ttgaccaaag tgattcctac ttggattgtc gataggaaat
     3301 tatttggatt ctgcaatatt tttaaaagct tttgagattt cacatctcct tcatcagctt
     3361 tttgctcgat tcgattctta ttgatcgaaa caacagcaat ttcggaagct gctaaaaaag
     3421 cattgatcaa tgtcaaaacg atcaataata aaatctgcgc taacagcgac tgactctcag
     3481 ggtcagcatt cataatcgca ttctctcctt ataaaattaa tttttagttt acgatagaaa
     3541 aaaacgtggc actatagcca cgttaaaatg gtatcacaaa gcaattttct ttgcaataaa
     3601 aacaagtgta ctaaaactac ctacgaggtc gtagcaattt tcttcttgtt ttcattttgc
     3661 ttgtcttctt tgacgatttt gattatatag gaaatgatcg tcttgcagat tgcgtaaaaa
     3721 ggaacaccta agaagatacc aagcaatccg gcaatatttc cagctactag aagaacgata
     3781 atgatcgtta atggatggat tttcagtgat ttcccaatta cattaggata aatgatattc
     3841 ccatccaact gttggacgat tagcacaacc acacaacaaa gtaatgcttt taccggatca
     3901 ttgaagactg ttactagaaa tgcaggtgct agtcctagat aaggtcctaa atatggaatt
     3961 aggtttgtca atcccgcaat gaccccaaat aagaaagcat aatccacacc aattaccata
     4021 tatcctaaaa atgtgaaggt accaacgaat aaacattcga ttgcttgtcc gctaatgtaa
     4081 ttagccaatg ttttattcag ttgccccaat aactccacaa tttggtttct tcttttttct
     4141 gggaaaaact gttggatgtt tggcactagt ttttctccgt ctttcaacat atagaacaag
     4201 atgaatggtg ctgtaatgat tactacggtt gccgaagcga tcgttccaac gatcgaacct
     4261 aaactattgg atactccact aaggaattgc tgaatgattg tcccgtaaga aatgtctaat
     4321 tgctcaaaat atttattgat atctacttcc ttgaacaaag gcaattcggc tacctctttt
     4381 aaccagcttt ctacctgttt gatgaaatca ggcatattag atgccaatga ggaaatttgc
     4441 tgtactagac ttgggatgac actaagtaaa atccaaataa ttgctgcaac taaaaggagc
     4501 agcacaattg cgacagccca tattcttttg atttttgctt tcatcaacag cttgacaata
     4561 ggattcaata aatagtaaag aaagcctgca atcagtacag gtgcaaataa cgtggaaaaa
     4621 aatgtcccga tcggttcgaa aataaagttt atttctgatg aaatcatgat caatgcagct
     4681 atgatcaata attcaagcga ccaaaacatt agtttagact gtctaagttt ttcaaacaat
     4741 agatttcctc ctttctttct tactagtata tattatattc gttaaaaatt cttttattct
     4801 ttttttattt ttgaaaactt gccatacttt ttatttgttt tattttccag tatactatac
     4861 gaaaggaagg tggctagacc atgaaagttg tacaaacaaa agatacaatg agcaatattt
     4921 acttagatgc tgtacggatc aggcatcaag tttttgtagt cgaacagggt gtgcctttgt
     4981 ctcgagaaat agacaaagat gaagcccatt gtatccactt cgtcctgtac tcagataaaa
     5041 aagaaccgca aggaactgta cgtcttttgc ctttagaaaa cggaaaaatg aaattacagc
     5101 ggatggcgat tctttctgaa tacagacacc aagggttagg aaaaatattg attgaagaag
     5161 cagaaaactt tgctaagaat caaggctata atacgatttt attaggggca cagtccacag
     5221 ctgaaacttt ttacgaaaaa ctaggatata cagcttacgg cgatcctttt gaagatgctg
     5281 gcatgcctca tatttatatg aaaaagactt tataaaaatt tatttcgaaa aaagcaaaaa
     5341 acagcaagct tttgagaaaa atctctcact ggcttactgt tcttttttgt gtttacttac
     5401 gcgacaattg cttgtaacgg ttataccaaa tatcaatata gccttccgag aacgggccat
     5461 ctccattatt gatccagtca accagaatct tcacattttc ttttaggatg aagtcaatat
     5521 cgttcgggta attcatcatt ttgctgtgca tctcatattc atcgacatcc aatagtcgtt
     5581 tctcgccgtc aggaaatacc ttgatatcca agtcatagtc gatatatttc aaggcctcat
     5641 cgtccagcaa aaaaggtgaa gctagattac aataatagga aacccccttt tctctgatca
     5701 ttgctattac attgaaccag tattttttat gaaaataaac aatcgctggt tcgcgcgtta
     5761 cccatcggcg tccatcagat tcagtgacta atgtgtgatc attcacgccg atcatagagt
     5821 gttcgcttgt ttttaatacc atggtatctc gccacgttcg atgcaatttg ccgtcgtgct
     5881 tgtaactttg gatcgtcaca aactctcctt cttttggaac tcccatagca acccttcctt
     5941 aagcagcatt ttgtgctttg tgtttgggtg ttcattacac cgacatcata attattatat
     6001 caataaaaat gagctttgcc tagaaaggat tcctattgtt tcgatttttc tctaactgat
     6061 ctaccagttt catttggacc ttaggaaaaa cgaccgaatc aaatgcatca ggcttcaacc
     6121 acttcgaagt tttgttctcg gtaaattctt gttctgctcc ctcggttgcc cgtccgtaaa
     6181 aaaggagaac atgccatttc agatgactaa aaatatgagt aacttctcct aaatgtcgtg
     6241 tctgccaaac aaagagttcc tgcttttcaa atgggagatt ttgctcttct tctgctacta
     6301 ggtcatcaaa cagatcgatt tctggctttg tttcccattc ttttttcaat cgctcatact
     6361 cctcttgtgt cacttccatc attggaaatg tccacatatt cgccagcaat ttttggctat
     6421 ctctttcttc aaaataatac gctccagaat gattttgaag agctgcactg atataataaa
     6481 cgtcttttgg ctttgctttt tttgttttta caggaaaact cgtttggatt cctcgtttgt
     6541 tagctaaaca aaaggcttgg atcggacaag tctcacactt tggagaagtt ggtgtacaga
     6601 tagctgaacc tagatccatc attgcttggt tgaattcacc tggatgcttt tcatcaatga
     6661 ttttccgcat cgcttcatca aagattttcc tgctagaagc ttttgcgatg tctgcttcaa
     6721 tgcaaaacaa tctgctgact actcgcatca cgttgccgtc aactgcaggt tctggaagtc
     6781 caaatgcgat acttgcaatc gcgcctgtcg tatatggtcc tattcccttc aatgagctaa
     6841 tttcttcagg cgtttgcggc atttccccat caaattctga catgatctgt ttagcagcag
     6901 cttggatgtt ccgggctctt gagtaataac caaggccctc ccatgctttt aaaagctttt
     6961 cttctggagc atttgctagc tcttcaattg taggaaacca ttccatgaaa cgataaaaat
     7021 aatcgatcac tgtgtctact ctggtttgct gaagcatgat ctcagagatc cagatacgat
     7081 aaggatcacg attatagcgc caaggcaggt ttcttttttc ttgttcatac cattgaatga
     7141 actgatcttg aaattctttt gtttctttat ctgtccattt ttcccattct ttattcactt
     7201 tcctcttcct tttccgtaat gaatcgctgg atcatatcca aatcaaatcc tttttgatat
     7261 aagctttgct ttacttttag atttcttttt ttaggttcaa accttaaatt tcttcgccat
     7321 agtttatctc cttgtttgac taaataatca tattctattt cttgatctgc ttcttgcgga
     7381 agatcagcta tcgcttgttg gatcacttct tgagaaaagc ctttcgtcat caatgtttgt
     7441 tggatttttc tcaataattc tttttggctt tttccgtgat tttttccaaa cgctttttcg
     7501 gctgtctgat gtgcattttg aacttggtct tcaaatgtat attggctgag cgcttgttca
     7561 atgatttcag gtttcaaacc tttttgctgc atctgctgcg ctaatttctt tggtcctttg
     7621 tcacttaagc gcatattcgt gcgcacataa ctttccccat agaccaaatc atcgacaagg
     7681 tccaattctt ttaagcgggc tacgatttta tttcgatctt cttgaaggat ttccttttct
     7741 tttaagtatg ttcggatttc tttttccgat cgcaactgat aacttaagta attcattgct
     7801 aggtgaaaac cgaaatcagc tccgctactc ttcttgatct cttggatagt ttcttcgtct
     7861 aattcccgtc cttttaataa tcgatagcgt actaatacat cttcagatac ttgtaaagct
     7921 tctccttcag ataaatctac gcgatagaaa gggcctttgc ctttactgat tctcgtaacg
     7981 gttaacataa acatcctcct cgaacatata ttctctatca taccacagaa aatccccgta
     8041 atcaggtatt atatgttaga ataaaccaca gaaaaaagaa aaagggagat acttatggca
     8101 gaaacaattg tcaaaactgg acaaacggtt cctttgaaaa tcaagcgttt aggcataaat
     8161 ggtgaaggaa tcggttattt caaacggttg atcatatttg ttccatatgc actaccaaaa
     8221 gaagaagtac tagtgaaaat cacgaaagca actcctcgct atgcagaagg agaattaata
     8281 aaagtcaaaa aaacaagcaa agatcgcgta gtggcaccat gtcctgttta ctatgaatgc
     8341 ggtggctgtc aattacaaca cttagcttat caagctcaat tggattttaa aaaggatctg
     8401 cttttgcaag cattggaaaa attcaaacca gcaggctttc gatcttatca acttttgccg
     8461 acgatcggga tggatgagcc ttggcattat agaaacaaag cacaatttca gttacgaaaa
     8521 aataagcaga ctcaaaaaat tgaagctggt ttatacgaag caaactctca tcagttagtc
     8581 cctataacag actgcttggt tcaagaacct cgaacacaaa aagtaatgaa tactgttgtt
     8641 caactattga ataaatttga tgcacccatc tatgatgaac ggacaaacag tggaatcttc
     8701 cgcacaatca tggttagaat cggtgttaaa actggggagt tacaagttgt ttttattact
     8761 cgcagtaaaa aattcccgca aaaaaacgca atgatccgtg agatcaatga acgactgccc
     8821 gaagttgttt caatcatgca aaatgtccag gcacaaaaaa cgtctctcgt gatgggggat
     8881 gaaacgatcc acttatgggg aaaagaagcc attgaagaac aaatcaatga agtgactttc
     8941 gatctttctc ctcgtgcctt ctttcaactc aatcctcagc agaccgaatt gctttatggc
     9001 gaaggaatca aagcattaga tgtccagcct aatgaaacga tcgtggatgc ttattgcgga
     9061 gttgggacga tcggcttaag tgtagcaaaa caagccaaag aagtacgggg aatggatacc
     9121 gttcctcaag caatcgctga tgcaaaaaaa aatgcggaaa gattaggata tcacaacact
     9181 cgatatgaag tcgggacagc agagcaagtt ctgcctaaat ggttgaatga aggttttcag
     9241 cctgatggta tcatcgtaga tccaccacgt acgggtttgg ataacgcatt gatccaatca
     9301 ttattgaaat accctccaaa aaaacttgtt tatatctcct gtaatgtttc tacacttgcc
     9361 agagatctgg ttaatttaag taaaaagttc aagattgaat atttacagtc cgtcgacatg
     9421 tttcctcaga ctgcacgttg tgaagttgtc gtgaagatga cgagactcgc tgactaaatg
     9481 aaagaggttg tgaaaaagtc tgccctgtat caagtaataa gaacagccaa acaaaaatag
     9541 ctcttcatgt tttatgaata ctgtacttgt gaccgaagag ttaacaggag gacactattt
     9601 atttaaaaaa agggttgtga catagttttt gtcacagccc tttttgatta ttattggata
     9661 ttcacgtcta tcacttgata aaatgcattt gtcgtatcgc tgatattcca tactgctaaa
     9721 atcacattat atccttttcg atcctgcgga agtgtgatcg tttggttgac tgtttttggt
     9781 ggaacagttg ctttgtcgtc tatttttgtg atcaattcaa aatcactaaa tttcaaaggt
     9841 tgattaggat tccaagttgg tttggtaatg tagtaatccc aagtacttgt ccgatgttgt
     9901 gctgttaaat tccaagtaat atttaatgtt cctggattaa ccatgttttt atgccatctg
     9961 ttagatgtct gttcatctaa aggctcaaag ccgctgactc ctgcactagc aatttttcca
    10021 tcaatgaatg tatttttggt agcttcgata ctttgcggtt cccattgtgc tcttccgaca
    10081 ttttgattta agtttcctgc tgctgatgtt cctaggtatg cacggcttcc tggagaagta
    10141 atataaccat gcgcaaaacc tggaatgcta aaaccgaaca accctaaact aactaaccca
    10201 ataaacaata acgtactaat ctttttcatg tagaaacctc cttcatttga taaccacatt
    10261 gtaaacgctt tattgtatta acacaaacat tatgctttta taattttatg ttgaagtaaa
    10321 aaaggtgaaa aaagtaagat gcagattata acagaagtag acgagaaaag tacaaataga
    10381 aaacatgaga atcaaatcat atgatctttt tcaaaaacat cattcatata aacaaatgtt
    10441 caacttttta ataacaacaa ttatcaattt ttttcttact tcacaaacta aatacaggtc
    10501 ttgctgtgag ccttcatggt aaactactat tatagaaagg agttagtaaa atgaagatga
    10561 taaaagatat tcgtttttgg gtttgtgtta taggaattgt catattaggc tttttatcag
    10621 ggttgcttag cggaaatccc ggagaatatt actattcttt gcaacttcca ccatttgcac
    10681 cacctagctg gatatttggg ccgatgtgga ccttactgta tatattgatg ggtatttctc
    10741 tttatttgtt acttaatcac aacaacaaaa aacagcgaaa caatcttgtc ggtctttttg
    10801 tgattcagtt catttttaat tttatatggt ctgctctatt ttttaattta agaaatattt
    10861 tcattgcagc aatcgatatt acacttttag ttattttctt gtcagtgctt atgtatcagt
    10921 tgtggctaca tcatcgcctg gcaatgtggc tcatgattcc ttattattta tgggtactat
    10981 tcgcaactct attgaattat tcaatttatt ttttaaacta agcgctaaaa agagacgtcc
    11041 tgtaatttaa cacatagaaa aagtgccacc taagttagat tttttgtcta atttaggtgg
    11101 cactctttat taaccaaact atttttttct agtacccact tgttgttcca tactcattat
    11161 tctggtcata accataacca ttttgttcct gtccagcgta aggatcataa ctttgagtat
    11221 cttgacttgg tgtatagtac tgacttgtat cttctgtttg ctgataatag gtattcggat
    11281 cttggtactg atcatatgtg ttttctgaat aggaagaata atcggtgtac ccttcactat
    11341 tcgatccatc accgtttgtc gaatcgccat cttccgaatc atcaaagaac atcattgaag
    11401 cattctcatt tttcaaatta ggtaatgtat cgcttgtttt tatatttaat tgtttcttca
    11461 gttcattttg gatcgaaaga agatcatttt ttcctaaaat ttgatagtag atatcattga
    11521 tcatttgtcc ttcgccttgc aactggtctt gtttgattgt ttcaaacgca ggggtgtagt
    11581 tcgtcgctac atcaagcatg tcatcccaat ctaagtccgt cgtcacattg ttctcaactg
    11641 cttttaaaat ttttctataa ttgcttacgc catctaaact taaaacttta ttgacgattt
    11701 tcgtcactac ttcccgctga cgacgttgtc gaccgatatc gccttctgga tcttcgtgtc
    11761 gcattcgaga ataggctaaa cctttttctc cattcaatgt ttgttcacct actggtacat
    11821 ggacaccatc caaagtaaag tcaattttat tattcacttt gatgccaccg accgcattga
    11881 tcaaatcttc catgccatcc aaattgatcg ttacataatg atcaatagga atatcgagca
    11941 gttgctcaat tgaatccatg gccatttcga caccaccaaa tgcataggca tgatttaatt
    12001 tatctaccgt tccataacct acgatattcg tataaatatc tcggtccaaa ctgatgatcg
    12061 ttgatttttt ttgtttcgga ttaactgtta ctaccatcat cgtatcagaa cgtccttgtt
    12121 ccgttcttcc taatccaccg gtatctaaac ctaatagcaa aaccgaaaaa ggttctttgt
    12181 catcaatgct tactctctca gaacgtttgg aggatattcg attagagttt ttagaaattt
    12241 tgtttactgt ttggctcgct tctcccataa atctgattcc gtatgctgat aatccggcaa
    12301 tcagcaaaac aaatacccct aacaaggtta atagaacttt ttgccatcgc ttcataatac
    12361 acctctgttt cacattttct aaaatattgt ctcatataac tgttcaatca gcaaattcac
    12421 tttttaatca taagaaaact ttaaaaaaca tttttgtgta acgattacta ttttttaaaa
    12481 tttctcaaaa ttcttaacta tcataccata ccgtgaaaaa gatcgaaaga ccgaatacac
    12541 gcagttcgca atcgttttat ctttttttac gtattttttc cagaaaaagc gaacaacaag
    12601 gtattttcca aataatcgtg cagtaaaatg atcgcacata cctttcccat ctttttaaca
    12661 gtctttgcct accgttcaga ttatcattcg atttcacgaa aatttcttcc tatgactggt
    12721 ccttttgtta aacccatctt ctattcgttt tatttccagc tcactgcatg gctcttaaaa
    12781 tgactcgaac tagtgatttc attaccatca tcttttaatg gatagatatg ctcctcttca
    12841 aagtaaaacg attttctttt aaagtaatag aattgacatg gtaatgagta tgtatactag
    12901 tggtttcaca aatggtcttt tattcaacat ccaaaaactg aaatttcttt aaaatccatt
    12961 actaacgaag aaaatgttcc ttgcttgata attagttagg acatgtcaag tgaacaaatg
    13021 catttactta gccaaaaaat ctaaaaactt cataaataga caaaaagaca cgactctcta
    13081 agaagtcgtg tctttattca acaaattatt ataatttagt cacttcagca gcttgaggtc
    13141 cgcgtgcacc ttcaacaatc gtgaattcta cttcttgacc ttcttctagg ctcttgaagc
    13201 cgtctccttg aattgctgag aaatgaacaa atacgtcatc tccgccgtct actgtaataa
    13261 acccaaatcc tttttcattg ttgaaccatt taaccgttcc gtgttccata aatgtatagc
    13321 ctccaaaaaa gttcttgcaa gtaaccttgc aagaacatta tacccaattt agaaaacgct
    13381 agcaagcttt cgctctttta ttttccaaat tatttttaag actgatatga cggaggaaat
    13441 ggttattttg ctgcttcgaa acgtttgttt acttcatccc aattaataac attccagaat
    13501 gctgcaatat aatctggtcg gacattttta tatttcaaat aataagcatg ttcccaaacg
    13561 tccaaaccta gaattggtgt ctttccttcc atcaatggag aatcttgatt tgcagtagag
    13621 gtaatagcta attttccatt ttccattaca agccaagccc atccagaacc aaatcgtcca
    13681 gcggctgctt ttttgaattc ttctttaaaa gaagaaaaat caccaaaagc ttcattaatc
    13741 gcttctttta tttctcctgt aggttcgcca ccagcatttg gtgccatgat ttcccagaaa
    13801 aatgaatggt tagcatgtcc gccaccattg ttacgtacag ctgtcttgat atctgttgga
    13861 atagcgtcca tatcagataa taattcttct attgtttttt cgcctaattc tgggtatttc
    13921 tcaattgctg catttaaatt cgtcacataa gtattgtgat gcttatcatg atgcagatgc
    13981 atcgtttctt cgtcaatata aggttccaat gcgtcatatg cataaggtag atctggtaat
    14041 gtgtaagtca taaaaaaatt cctcctcaat gttttcttta taatgtctac attacaaaga
    14101 gtaacaaaga gaaggaaatt cttcaattgt tatgcctagt cgtctttttc actttgacct
    14161 acttcttctt caaaaacctc agtttcttta aaaataaaca acttgctgaa aatatagttc
    14221 gctaggataa caataatttg agtgatgatt ttcgccacat actcattgct gttcataaca
    14281 ttgatcaaca ataccattgc ccccatgtcc aatccgtaag aaaggatacg gtagaagaag
    14341 aacttaccgc attcaataag tgcatgtgta aaattcggtg attttgaatg gaatacccaa
    14401 agtttgttcg ttacaaaagc aaacaataca gaacaaaacc acgaaataga attggataag
    14461 gccatactta aatcaaaata ttggcgtgcg agagcaaaac taacaaaatt tacaatcgta
    14521 gccaaaccac caaaaaagag atagactagt atctcccaat atccttttgc ttctaaatat
    14581 ccttttaact gtcgatactg cttcatgagc caacctacct tattaaaaat tcttatttat
    14641 catactctat atttcaataa ctttcatctt tatttaaaaa aattaatttt ttttgatcct
    14701 ttgcctttgt ctctgcttgg atcgtcacat gtgtaatacc atattttaat tttaataaag
    14761 aaattatacg aggatatatc ttttcaatct cgcttagtag ctgatcttca aaatcaatat
    14821 gtccgtcgaa aataattatc ttttcgctat actgccaaat atggacatga tgaacattct
    14881 ttaccccatc gattgcttca atttcttgtt ccattttttc atagtcaata gcaggtgcac
    14941 tttgtatcaa aatagcggca gcttttttga tcaccattat cgcttctatc aatatatata
    15001 tcgaaatgat cagtgtaata actggatcga tccagtagat atcgaaaaaa cggatcaaaa
    15061 ttgctccgat aatcacactt accgaagaca gtgtatcact taacaaatgc aaataactag
    15121 attttatatt caaactttca tctgcttctt gtcgtaataa taatactgag aacaaattcg
    15181 cgagcaatcc aataactgct actgttagca tcaaatgact atttatcttt tctggtgaag
    15241 tgaaccgctt gaatgcttca acggttaaaa acaaggataa tatgatcaat atgaatgagt
    15301 tgatgaaagc actcaatatt tctaaacgct ggtagccata cgtacgtttt ttatttgctt
    15361 cttttcgggc cattttttgt gccccgtaag aaaaaataat tgccaatgta tcactaaggt
    15421 tatggaaagc atctgaaaca agagacaaac tccctgacat aatccctcca ataaattctg
    15481 ataaagtgat caccgcattc aaaaccacta ctccaaatag tttttttgat gatctctgtg
    15541 gctgtgtagg cactattcct tcctccattt tatttatatt ggatagtctt tagacaagaa
    15601 taacattttt ttgatatttc accaatcatt cagatgtttc cctaaagcac ttgtatttca
    15661 cttgatactt cgctagaatt gcaagtgatg acactactag atgaggagta cttatgacaa
    15721 caaaacaatt aattatcagt tcctttatac gattcaaaga cctaaaggag acgagaaatg
    15781 tcccattttg gaaagccatt gtctatcttt tagttttgag tataattatg gctttgccaa
    15841 tctcttttca agtttttcaa gtcttaaaca acattcaatt ggatgggcaa aagattgcta
    15901 ctcgcattcc tgattttaca atccaaaacg ggcagcttca aaccgaagaa aaagaaggat
    15961 tcatttacca aacaaactcc attatcttca catttgatcc agagggaaaa cgaacagaac
    16021 aggacatctc tactgatttg atggggaatt ttgttagtgt cgggatgtta aaaaacaaac
    16081 ttatcatagc tcttcctaat acaggcacga cttctgcttt gttgaacaat aaccaattag
    16141 aacttcccta caccaatgaa tctttaaaaa acttaacagg taaggagctc cgttcttttt
    16201 taagtaaagc atctatacca ctttggataa aagtcctgac atttatattt tctatttatc
    16261 ctagttttct gaatctgctg atcactttgc tatttaccaa cattgctgct tttctctatg
    16321 cacgctttcg actaacaaaa gctacttttc ttgattgttt aaaaacattg atctattcaa
    16381 tttcattgcc tactataatt gcaacaatct tgatggtctt tcttccttct tttgacacaa
    16441 gcgccttcat tgcttttgca ggaatcttta tttttgctca agctgtcaaa ggatgggcaa
    16501 aaatatcgat caattaagaa ataaagacaa ctcgttattt ctacttcaat agaccctttt
    16561 agaggttgtg acattttttt gtcacagccc cttttttatt tgaaaaatat ttctttctca
    16621 aaaaacaagc aaggcgcttc aaagttcttc ttgtatgcgc tacacttaga gtaaagaaag
    16681 gcgggaaatt aactatgtca gaaagaatca tttatgcaga tcgtccattt cgtgatgaat
    16741 ttatcgaaga aatccatact atagcgcctg gttatatctt taaaacagaa ttacaaccag
    16801 aggatctaca aaatgtagag atcagtttgg gctggaactc agactaccaa gaaaaactat
    16861 tagcctcttc taccttgaaa tgggtccaat ctatctcagc tggtgttgat cagctacccc
    16921 ttgagacttt ttcaaagaaa catattttac tttcaaatgg tagtggcatc cacagtgaat
    16981 cgattacgga gcatgtctta ggaatcatcc ttgggtattc tcgtggtctt tttcaagcac
    17041 aaaaagcaca gagtgacatg aaatggttag gttcatcggt ccattaccaa tctatccaag
    17101 gaaaaaaaat actaatcatt ggaactggcc atattggaaa aatgattgca aaaaaatgca
    17161 aagcattaga catgaactgc tatggaatca atacatccgg tcatccagca gaaggaatgg
    17221 aaaagacttt ttctttaaat caactaaagg aagttcttcc tgaagtagat ttcgtagtca
    17281 atatcttacc tctaacggag gaaacaactg ggctctttga tcagcatatg tttgaacact
    17341 ttgatccaaa atctgtattc atcaatgttg gccgtggtgc ttctgttaag acacaagatc
    17401 tggtgcaggc tttaaataac cagcaacttt cctttgcagc attagacgtt tttgaggaag
    17461 agcctcttcc acaagatcat cctctttgga aaatggacaa tgtactgatt acttcccaca
    17521 ttgcaggttt gacacctgat tttcaaaaga aattaatggc tattttttta accaatctga
    17581 aaagctactt tgccacccat gagcttcaaa ccaatcaagt aaaattgtct gcaggatatt
    17641 aatagctgaa aacaatattt tcaaaagaac aaagagaagg tgacaaa
//
LOCUS       DACEUZ010000048        17517 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.048, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000048 DACEUZ010000000
VERSION     DACEUZ010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17517
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.048"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            100..2427
                     /locus_tag="IXG44_001388"
     CDS             100..2427
                     /locus_tag="IXG44_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ1658115.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSATETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            2553..2882
                     /locus_tag="IXG44_001389"
     CDS             2553..2882
                     /locus_tag="IXG44_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HAQ1658116.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     AEIKTSD"
     gene            2922..3926
                     /locus_tag="IXG44_001390"
     CDS             2922..3926
                     /locus_tag="IXG44_001390"
                     /inference="COORDINATES: protein motif:HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAQ1658117.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKTKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            3923..5352
                     /locus_tag="IXG44_001391"
                     /pseudo
     CDS             3923..5352
                     /locus_tag="IXG44_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306286.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
     gene            5558..6730
                     /locus_tag="IXG44_001392"
     CDS             5558..6730
                     /locus_tag="IXG44_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ1658118.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(6892..7347)
                     /locus_tag="IXG44_001393"
     CDS             complement(6892..7347)
                     /locus_tag="IXG44_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1658119.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQQAAAEQASQQAQAAAP
                     EQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSKCYGN"
     gene            7936..8358
                     /locus_tag="IXG44_001394"
     CDS             7936..8358
                     /locus_tag="IXG44_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ1658120.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            complement(8524..9717)
                     /locus_tag="IXG44_001395"
     CDS             complement(8524..9717)
                     /locus_tag="IXG44_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1658121.1"
                     /translation="MTTYQRKLLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            9941..10318
                     /locus_tag="IXG44_001396"
     CDS             9941..10318
                     /locus_tag="IXG44_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ1658122.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKKIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            10306..10644
                     /locus_tag="IXG44_001397"
     CDS             10306..10644
                     /locus_tag="IXG44_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ1658123.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            10778..11221
                     /locus_tag="IXG44_001398"
     CDS             10778..11221
                     /locus_tag="IXG44_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HAQ1658124.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            11483..12298
                     /locus_tag="IXG44_001399"
     CDS             11483..12298
                     /locus_tag="IXG44_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1658125.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            12308..12970
                     /locus_tag="IXG44_001400"
     CDS             12308..12970
                     /locus_tag="IXG44_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAQ1658126.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            13175..14839
                     /gene="alsS"
                     /locus_tag="IXG44_001401"
     CDS             13175..14839
                     /gene="alsS"
                     /locus_tag="IXG44_001401"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HAQ1658127.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            14852..15562
                     /gene="budA"
                     /locus_tag="IXG44_001402"
     CDS             14852..15562
                     /gene="budA"
                     /locus_tag="IXG44_001402"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HAQ1658128.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            15692..16189
                     /locus_tag="IXG44_001403"
     CDS             15692..16189
                     /locus_tag="IXG44_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HAQ1658129.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            16374..16634
                     /locus_tag="IXG44_001404"
     CDS             16374..16634
                     /locus_tag="IXG44_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1658130.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(16996..17235)
                     /locus_tag="IXG44_001405"
     CDS             complement(16996..17235)
                     /locus_tag="IXG44_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1658131.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
BASE COUNT         5944 a         3045 c         3474 g         5054 t
ORIGIN      
        1 aaaaaagccc caaactttct atacatcaat aaaataataa aaattaaatt tagaataacg
       61 tgtaaaggat tatttgatga ttttttattg agaggaggga tgaaaatgat taagaaaact
      121 caaaataaaa tccaacgtat atgtcaaaaa cattttgttt ctagactcaa agctgtatct
      181 aaaagaaaat acttacatcc cattatgatt tcgatcatct tgatttttac tttcttaggg
      241 agcttatcaa gtatttatgt aaacgtttat acagaaacgt caaattttgc taaccaaacg
      301 acagaggaga caaatagtcc accctattct gaaatacagt ccatagaaag caccaatcag
      361 tctagcaaca acgagacaac ctcaaccaca tcctcgactc aactgattga tccgacagaa
      421 aaagaaatca cagcagcaaa agcagcaggg gagaaagctt attcagaaac aggtagaagc
      481 cagacattta gtactgttgc ggccactgcg aatttcaacg gacataccat accagatgga
      541 tatacagtga tatatgccaa agatggagtc attgttgcgg ttaacggatc gatgagtgcg
      601 gataaaactg cgttacaagg cgtaaccaaa gcaatcgctt ttattgataa ccgtccagat
      661 atccataatc tgtttattgg ctccacagta aatggtacta ctatcacatt cccaaaaagt
      721 atctatatgg gggtggctac cgctggaaat acgatcgatt cagttacacc caatgcggat
      781 aaaagttatt cttttacacc ctcaacatgg ggagggcttc ctgccggagg aggtatgagt
      841 actttttcaa tcggtataca gttagcaaat gatattccat tcacggggta ctcgatcaaa
      901 aatgggcaga tcgttgatgg taagggaaat gtttcaaatt ttccaaccta tcaagcagca
      961 acggttcagg tcaatgcgaa tcggacagga actttttctt atacagtcaa tggtggagca
     1021 gcaaaaatcg gaacgtacgg gactacatta tctggaatcc atgtgggtga caaagtggtt
     1081 atcactccaa cagcagtaac gggctattct tttgcacaaa caaacggttc tttcacagcc
     1141 ctagaaagta acgtcgatac ggtgacctat acaggcaaac aaacaccaat cactattaag
     1201 tatcaagact attttggaca gatgattgct ccttctaaaa taatgaatct tacttacgga
     1261 agtgctgcac aagacctgac gactaatacg cctactatca acggatatac ctttacagct
     1321 gtttcagcga cagaaacaaa gaaccaatca gcaatgagtg ttttagctag tttagataca
     1381 aatggaaatg taatcgtaaa ggacgcaaat ggaaaacaga tttctgaagt catcctatat
     1441 tataagacaa atgcaacagt atcaatcaat gcaaatggca gtaagtacta tgatggtctt
     1501 tctgttgtac cgatcgttaa atatgccttt aatgataaaa cagaatcaac atccctttta
     1561 aacaatgatc tcagtattga tcaaatcaca tggaatccaa ctgatttcaa agcgatgaat
     1621 gaaaagggag ttgaagtaac tgcgcctact gaaattggaa cctatacgtc ttggcaattg
     1681 acaaaaagcg gactagctaa actagctgcc agaacaaact atttatttac tatcgtgcag
     1741 acatctgatg tctatacgat taagcaaatc agcggtgcgg tagagctcag tggcagtaag
     1801 acatatgatg gaaaagcagg tgtcccgtca atccatgtca agctagcaga gggtgtcact
     1861 agttcgctta ctccagtaca agtggcactt agcagtatgg attacacagc agatgcacaa
     1921 tcagcaaaaa atatggttaa tgctggcagc tatacaatta aactaacgaa tagcggaata
     1981 gacaaagtca aagcagcaaa tcccaagttg tcatttactg atatagccaa cacatcagga
     2041 acttatacca tctataaagc taacgcagtg attactgtcg atgacgctgc ctttaattat
     2101 gatgcccaaa gtcactcgat ttcggttgga aatctccacg ttaccggtgt tgtcttaggc
     2161 gaatcattgg attataccct aacagttaac agccggacag atattggatc acaaatagta
     2221 acgattagtt taggcgaagg aagtgtaaac aataattata cgattatcgc aacagatacc
     2281 gctaagttga cgatcaatcc gactctcttg cttccttcga caggtgggat aggtctactg
     2341 ccctttgtct ttttaggact gatctttatt ttttctggct tcttttattt tatccatcgt
     2401 aagaaaaaag caggtgaaca gcgatgaaaa aaacaaaaaa tgtacgtgtg gatatatttg
     2461 ctgttctctt ttttatccta ctggtctttt ttataggttc tccgactgtg tcagctagag
     2521 aatcacctat tacatttact ttgcataatc gaatgttcga aagcgataag atgccaagta
     2581 tcagtcaaaa cgacggaata aatatcccgg ataactttca aggagtaaaa gggttgaacg
     2641 gcgttacttt cgctatatat gatgtctcgg atgaatttta caagctgcgg tcagaaggaa
     2701 gtagtgtgga agacgcacaa agaaaactag cacaaaaaag tgatagtgaa aaaatactgg
     2761 cagaaaatgt gacaaaaaca gtagacgagg aagagggcat tgcttctttt agtgcatcgg
     2821 acaaagatga gcaaggtcgt gacgctgttt accgatttgc ggaaataaag acttcagatt
     2881 aagtaaaaga aaaatcagca ccatttgtcg ttgtgcttcc tgtgacagat tcatctaatc
     2941 ataaattgac gaccatccac ttgtatccaa agagcgaaca aaaaatacct gctgcactca
     3001 cattaacaaa aacagtagag aataagcaga cagattttgc agatggggat aaagttcctt
     3061 atctcatcac cgtaacaatt cctgagaaca tcaacgagat aggaacctat acgatcaaag
     3121 atacagctga tcctcagtta tggctagaac cagaaacgat cgattttttg attggtggag
     3181 acaagattca tacatttcac acacaaaaaa caaaacatgg ttttgtctta tcagattctg
     3241 gaaaagatct tagttcgttt gctgggaaaa aattaacgat ccgttaccaa atgacattga
     3301 aagaaaacag tgaaaaaacg acatttgata acgatgtacg tttgacgact gacaatcaaa
     3361 cagtagaaac acatcttgcc atccaaacag gaggcaaaca atttgtcaaa gtggatcggc
     3421 aaacgaaaca aaaactagca gatgctcaat ttctagtcaa aaaaaatgac caatacttag
     3481 cgcaagaaaa cgggataaat cattgggtag ccaaagatga ttcaagagca acgattttta
     3541 cttccagtaa agatggaaca ttttctgtcc atggattgtc ttttggaagt tacgaactag
     3601 tggagattaa accgcctaaa ggatatatat tgagtcaatc aaccattctc tttgaagttg
     3661 aaaaaggcag ttatgatccg cttcatccta taccattgga aatcatcaac gaaccaaaag
     3721 caaatccgac gaataatgga aaaacgccag ttcagatgca gacaaatggt ccatcaaatg
     3781 aaaaaaatgg aggaagcttt ccaaaaacaa gcgaagagat gcttggaggt ttctcaattt
     3841 tagggttgtt gctagtcctt tcaacaggga cagcttggtt ttataaaaaa caacaaaaag
     3901 gaaacaatag gagggaaata aaatgaaaaa catcaaaaca aaaatatgga cgtttttagg
     3961 aactgctatc atgctgcttc ctttcgttct cggcctagga actgcagagg tttctaggcc
     4021 gtatcaccta caccagaaaa tgttacggtt aatttgcata aattgaaatt cacttcagca
     4081 ccagaaaatc aaataaacaa cggaacggaa ttgacatttc cgaatagcga accgttgaac
     4141 ggcgttgaat tcaacgtata tgacatcact gctacgtact atccaagtaa agacacagca
     4201 gttccagcag acgctacacc cttcgcaagt gtaacgactt ctggtgaagg attagctaac
     4261 ctgactcttc caggtaaatc tgatgggaaa gatgctgtat acgtctttgt agaaacacca
     4321 aagcccggtg tcgaaacgtc gccaaatatc gtgttaagct taccctttta taacccgaat
     4381 tcatcagacc ctgacaaacc tttagacacg attcacctat atgctaaaaa tgtcatcaaa
     4441 gaaagcaata tcaaaatcca gaaagctggg aacttttcag gggtagataa gttagcaggg
     4501 gcgcaattcc agatcaaaaa ttcagagaat caattcgtga ccggttttga tgaaaacact
     4561 ggtgtcgctc tctatgatcc tgctgagtca gcgttgacat tcacaactga tactgaagga
     4621 tccatcagtg tcaatggatt aatagacggc aattatactt tagttgaaac aaaagcacca
     4681 gatggttatc aattaaccaa tccacaaaaa gaaactactt ttatagtaaa aaacggaaaa
     4741 ttgcaacaaa caaccggtat catccatggc gcagatgaag ccaatcttgg gaatccaaac
     4801 ggtgtagtca atcgggaaaa tacaactacg gataacacga ttactgtaga aaatgtccaa
     4861 aactacggtg atttccactt catcaaacaa gatgtgaaca cgcaagaaaa attagccgat
     4921 gcagaatttt ctattctaag agcaaaagat ccaactgtta aattaatgaa aaaaatagca
     4981 aatcttgcct caggagaata cccatatgct tggtcagatg aagtcacagg ttctgactgg
     5041 gaagaagtga aattgatttc tgattcagaa gggaaatttt caatcgaagg gctgaaacaa
     5101 ggggcatact gccttcaaga aacaaaagca ccagaaggct acgcattacc taagaatgaa
     5161 gctgcgtaca ctgaatttac agtagatgat gatccaacaa cttctgatga aacaacgatc
     5221 gataaccaac ctaaaggaat cttgccttca actggtggta tggggatcat cgtattcatc
     5281 ttggtaggta ctgctttagt tggaggagca gtgatctact tcaaaaaacg ccatcaagaa
     5341 acagaagctt aaactattgg aaacaaaaat catacgataa ataaacgata gtgattcaaa
     5401 tggggagaaa ggttgtgaca agtggtcggt ctcttggtat tcgctcaaca aacggcaaaa
     5461 taggaggaac tattttgccg tttgttgagc gaatactcag gtcaaggcgc tgatttcaca
     5521 acctcttcat atacatatta gaaaataggt ggacatgatg aataaaagca gaataagaat
     5581 actggacatc ttcatggcaa tcattttagt cgttgggatc gggatctttt cttatccttt
     5641 tgtagaagat tcactgaatg attttcttgc ccagcaaatg atcatccatt atcaaaagca
     5701 agcatcgaat aagaattcag aggaaataaa aaaacagcag gaaaaaatga caaagaaaaa
     5761 ccaacagttg gcagagcaaa acgtatcacc agggataggc agtttcaacc aagcagttga
     5821 tgcaaaagca ttaaaagatt tacctagtaa tgcctttttt atggaacata tgcttggagt
     5881 catcgaaatc ccaaaaatca atgtcagctt acctatattc gatcagacaa cggaaatatt
     5941 tttgcaaaaa ggaacgtcat tattggaaga aagttcttat ccgactggcg ggaaaaacac
     6001 ccatgccgtt ctttctgggc atcgtggact cccagaagca aaattattta cagatttgcc
     6061 taagttgaaa aaaggagacc agtttttcat ccagatcaat ggaaagacat tggcgtatca
     6121 agtagaaaaa atccaagtgg tcttacctga tgaagtagat tctttgggta tccaaaaagg
     6181 cagagatctc gtgactttgc ttacctgtac tccttatatg gtcaatacac atcgactgct
     6241 tgtgacgggg catcgtattc cttatcaagc aaaagaggca aaaaaagcga tccaaggaat
     6301 tgatcaatgg aaaaaatgga aattttttat ttggtttatc ggtatactac ttggatcaat
     6361 cggcttagtt tggctactca tcgcttattt agattctttg gccatcgcta agcgaaacta
     6421 tcctttatct ttttatgtaa agaacacaaa cggacgacca atcgaaggaa tggtcttttc
     6481 tgtaaaaacg ctcaacggca aacactatat tactagagaa aaagttccat ttgtgaaagc
     6541 ttctgatgaa tacggactag tgagattttc tgatttaaaa ggcaggaact atcgcttgca
     6601 acatgaggag ctcctgctta aaatccatgt aaaacataaa cattcgaaac agttttcaat
     6661 gaaattgaaa aaaggaagat ataaactgcg aaaagaaaaa gaggtgtact atttgattga
     6721 gaaagaatga atgaagatgt ccaaaaaagc agagggaaca gactaaatga aaaaagagag
     6781 aagctgacga aatggacgtg taaactgcta tttttggttt actatctgga acgccagctt
     6841 ctattttttt atgtttcaga agaattttat tattatagta taacttttga attagtttcc
     6901 ataacatttg gagcaagggg ttaaaccacg agcttgagct tcgcttaaag tggcaggggt
     6961 atacgtacca tttccacatt tatgtgtatg gtattttgaa ccggtcggtg tgacatagac
     7021 agtttgttca ttattttgct caggagctgc ggcttgtgcc tgctgagagg cctgttcagc
     7081 tgctgcttgc tgtcgcgcct gttctgcttg ttgctgtgcg attgcttctt gttgcgcttg
     7141 ttctgcttgt tgtcgtgcaa tttcttcttg ctttttccgt tcttcttctg cttcttttgc
     7201 tttgattgtg gaatcaattg cagcaagacg gcttgtcaac tcttgtttgt tttcaggtag
     7261 cgcttggata gctgctgaag cagttgcata gttttctttt gtaggatttg cttcagcttg
     7321 tgacaaagca gattccgctt ctgtcacttt tttttctagt gcagcttttt ccgctttttg
     7381 tttctcttct tcttttttct tttcaatttg ttcttgttct ttcgatgaat ctgccgtact
     7441 ttcggagatc gtggtactgc tgctggaagc ctgagctgaa cttgtgcttt gagtagtaga
     7501 cgtagtagca ccacacccac tcaacaaact tcctgcgacc aaaaacaatg caactaattt
     7561 tttcactaaa tttcctcctt aagtaaaaaa caaccgtttt caataaatgt ccttttcaat
     7621 tgtttactta cttgttataa taattgtgtt atgaatagtg attgaggaaa cttaatctaa
     7681 tcccgttttg atacacggga ttttttgcta tagttctgtt attaagtatg caataataaa
     7741 atatgtacgt tattttatta ttagattatt gacatttata taataaacga ttaaatgatt
     7801 catatcaata gaatacttag gaaaaaaaga gaaataataa caggtttatg cactattgac
     7861 ggtgtgacaa tatgatattt gtatccgttt ttttcttgta tgtagtatag taaagtagat
     7921 acaggaggag aaaatatgca gactttttta acaaaagttt ctcaaaagaa aattaaaatg
     7981 attcatttac tgcttgaaac agaacgttgg tgttcaatag aagaactaca aaacgaattg
     8041 aaggtatcaa gtaagtctat cttgaattat ctaacagatt tagaagaatt gttccaaaaa
     8101 tatcctgata aggtcatttt gaagaatgag aataatcggc gcttttctat ggaaaagcag
     8161 gagaacttcc caatctacat tatttatctt catttctaca gaaaatcata taattatcat
     8221 ttgattgaat tcatgtatca acatcctgag aaaaatttgg aagattatgc agacgctcaa
     8281 tatacgagtg tatccacagt ttttcgttat gctaagttat tagtaaagta ctttgaaatg
     8341 acggacgtca aagaatagta gccgccagat agaaggaaat tagcagttga aaaagctgcc
     8401 ctgcatcaag taatacgacc agccaaacgg aaacagtgtc tcatgttttt gatttggctg
     8461 gtcttattac cgcagatgca agcttttgcc acacctcttt cttgtcattt acagactcaa
     8521 ctatcaatgc tgcaagcttt cccttaatgc tgtaacacca gtaagagttg tcaagaccag
     8581 cagactgact aggtaaatac cgctcaaaaa caagattacg gcagttaaac tataatgatc
     8641 catcaagaaa ccgatgacga tagaagaaaa accaccaaca gctcgtccaa ggttcataat
     8701 gaaattattc gccgtcgcgc ggatttctgt tggatacaaa ctactaatca ctgcgccata
     8761 tccaccatac atgccattga taaagtagcc gactatcaca gcggctgcta ccaaactcca
     8821 ctggctatgg gctaacacaa gtagatagac agataaggat gaacaaatta aaaacaaaga
     8881 aaaggctagt ttaggcccca atttatccat gattatgcca aaagtcatca tccccaaaga
     8941 catgcctaaa atagtactga ccatccacaa tgaagaccca gaaaccgaaa gccctaatcg
     9001 attttgcata attgaaggca gccaattcat taacccaaaa tagccggcaa tttggacagt
     9061 taccatgata ctaaggccac atgtttgcca agcaagatgt tttgattcaa acagtctgct
     9121 aagtttggtt ttcttttcgg cagattgtac agatttgaat tcctttggtt cagtcaagcc
     9181 gcgacgaatg aacaagacca tcaacaccgg cagtacacct acgacataca acatcttcca
     9241 accaaacgtt ggaatgatca cagcagctaa aatcgcagcc aaagccgcac cgacctgcgc
     9301 accaatacca gcaaccgaag aagctcgtcc aatttgtttt tttgaaaaac tttcggaaat
     9361 caaggacata cacgccccgt attctcctcc cgctcctaca cctgcgataa agcgaaagag
     9421 ataaaccaag tagatgtttg aagcaaaata catcaataaa gaagcaatcg aaaacagaag
     9481 gaccgtttga gaaaatactt tcacacgtcc atatttatca gctatgatgc caaaaaaaat
     9541 acctccaatg agcatcccca aattagtgat cgttgaaatg aaacccgctt gcgtaccact
     9601 aatatgaaaa gaagtaatca ttgaagataa tgaaaaagct aaaaacataa tatccatatt
     9661 ctctaaagca attcccgccg atgtcgataa taataacttt cgctgatacg ttgtcatttt
     9721 tatacccctt ttcctaaaat ttccctaaat tgttaaaagt atatccaaaa aagaataaaa
     9781 aaacaagact tttttatttg aaataataca caaacaaccg atatagataa tccagcttag
     9841 ttttaataga aatgagtatt gacttggagt acacttaaag ttgtagaata aataaaatac
     9901 ataagtaaag tggcaagaaa ttaagagaaa gagtgatcag atgaatatca aacaagtcag
     9961 tgaagaaaag ggcatttcgg ccgatacatt gcgctattat gaacgaatcg ggttgattcc
    10021 accagttaat cggacaaaag gcggaatccg tgattataca gaagaagacc taaaatgggt
    10081 cgactttacg atctgtatgc gaaatgccgg attatcgatc gagtctttga ttgaatatat
    10141 tcgcctatct agcgaaggag aagcaactgt atttgagcgg cgtaagcttt tgattgaaga
    10201 aagtgaactt ttgacgaaaa aaattgctga aatgcaagaa tgtttggaac gtttacaagg
    10261 aaaaatcaag aaatatgatc gtgtgttatc tagaaataaa atggaatgta tcaagtaaaa
    10321 aaatcaagga gcatcgctat ggagttacgc ttactaaaat atttttggac aattgcagaa
    10381 gaaggaacaa tctctcaagc cgcagaagtt ctacatctta cacaaccaac cttaagccga
    10441 caactgaagg agttagaaga ggaactagga acagaactgt ttgtacgcga aaaccgcaaa
    10501 atgatcttga cagaagcagg ttattttcta aaaaagccga gcagaagaaa ttttagattt
    10561 aacgaatcaa accactcaag aattcgagaa cagaaaaaaa caattattca gcggccgatt
    10621 tacgatcggc tgtgtggaag ctgataattc gaatacattg gcactattac tagaaaagtt
    10681 gatccgtgat tatcccagcg ttcaatttca tatccacagc ggaacaagtg atgatatcac
    10741 ggagtgacta gaaaaaagcc tgttagattt agcgattttg ctagatccag tagatacaga
    10801 gaaatacgaa acgattttgt taccaagaaa ggaaaaatgg ggattgctca ttttagaaaa
    10861 ttctgtttta gcaaaaaaag acgtgataac aaaagaagac tttgcgaatg tacctctttt
    10921 gatctcaaag cgtccagaaa tacagcgaat gtttgctgac tggttaggca taaaaaagga
    10981 gcagttgctg atcacaggaa cgtacaattt gattttcaat gtatttgcgc taattgagaa
    11041 tcaggtaggt gctgctttag tcatagaagg tgctgtaagt catctttcaa agcgagtgac
    11101 attcgttccg cttgacccaa tgattgaaac gaactgcatg ctagtctgga aaaagaatcg
    11161 gattttatca cctgtagtca atgaattgat tcaccgtttc aaagaagctt ttgaacagta
    11221 ataagtatct ctaatcgaag aaatttcaat acaaaagcga gacgtatgac agtcttgctt
    11281 ttgtattttt tcatttttaa atcaaaaaaa ataaagagat ttacataact tgttaaataa
    11341 gcgcttacaa acataagaag aacggaaatg aatcagctat aataaccgtt gttctggatt
    11401 gtaatcatta caaagaaatg aagattataa ctttggaggt gttcgaaatg aaagaaaaaa
    11461 agcaaaaatt cgttcaaaaa acatgccctt tattcaatgg ttgggtatga ttctgctgat
    11521 tgtattagct ttattgacga tcattccttt tttatggatg gtttctgctt cattcaaagc
    11581 aaataatgaa gtgttcacga ttccgattca atggatccct aaaacttggc atttagagaa
    11641 ttatctggtt atttggtctc gtattccttt agtgacattt tttaagaata cagctttttt
    11701 aagcgttgtg atcacgttga ttcagttgtt tacttctagt tttgctgctt acgggttttc
    11761 taaactaaat tttaaagggc gcgatatttt atttttagca tatatcggca cgattgctgt
    11821 cccttggcag tcttatatga tcccgcagtt cattatgatg agaaaagtgg gtttaacata
    11881 tacgttgtgg tcgttggtgc tattacaagc gttcaatgct tttggtgtgt tcttgttgaa
    11941 gcaatactac agtagtatcc cagattcttt atgtgagagt gcgcggatcg atggcttatc
    12001 agagtgggga atctatcgca agatcatttt acctcttaca aaaccggcga ttgccagttt
    12061 gacgatcatc acattcgtta atacatggaa cgattatatg gggccattca tttatttgtc
    12121 ttctacggaa aataaaacaa tccagttagg gttgaaaatg tttattgggc tatttgatgc
    12181 agagtacgca ttaatcatgg ctgcctcagt tgtttctgtt cttcctgttg cttttatatt
    12241 tttagcgatg caaaaatatt ttgtagaagg gatcgcttca tcaggaatga aggaatagga
    12301 ggtgattatg aaagagcagc gttttgacaa aaatgtttat atggaattct tttattggtt
    12361 gtatcaatca ttggcaagta gcctttgttt ttggtgtgtc aatatcttgt atatagcagc
    12421 tgcgttcctt ttgtcgatag atggaaggaa tgtcttttcc tttatttttt ctttactttt
    12481 tattggtcca ggttgcatcg tgatgatagg tatgacagaa gaattagcaa aagaaaaaag
    12541 tttttcacca gtacgtttgt tcataaagac gtttcgccaa ttttggctaa agggattttt
    12601 ttattggcta tttgcttgga tagttagtgt gataatgatt tttgattgtt tcttttttat
    12661 acgtttttct tatggaaaat ggttgattcc gttgtttgtt ttacttgctt gtttatcagt
    12721 ttcctttagc atcaactact ggtattttca ggtgagaaat ccagcgtcga aaccaaatca
    12781 agttctacgg atagcgttct actatacgtt gaaaaaatgg tatgtgagtt tactagattt
    12841 tttactattg acaagtttgt ttctcttctt tttcgtaaag ccccaatggt gtattctact
    12901 aggtccttct atcgtatttg gtttgattta cttcaacaat cgtaaattga tgagaactat
    12961 ggatctgtag actttataaa ttgaaacttt caattaagaa tgacagtttt tatgtaaaaa
    13021 ctgtcattct ttttcatttt ttgaaacata tggctaaaca aatgaaataa aacgtgctat
    13081 acttttgaat gaaacaggtt tagaaactgt atcaattatt tgtgattatt tctaactaat
    13141 aattaaaaat aaagcataaa aaggacgatg ttcaatggac gaaaaaatga aacaaggttc
    13201 gaaatctgta gtagaaagtt tgattaatca tcatgttgat tatgtattcg gtataccagg
    13261 tgcaaaaatc gatggtgtat ttaatgaatt agaggatcaa ggtcctgaat taatcgtgac
    13321 tcgtcacgaa caaaatgctg catttatggc acaagcaatc ggacgtatta caggagagcc
    13381 aggagtagtt atcgcaacaa gtggtcctgg tgctagtaac ttagctacag gtttggtaac
    13441 agcgacagca gaaggtgacc cagttttagc aattgccgga caagtaaaac gaagcgatct
    13501 attgaagttg acccatcaaa gcatggataa tgcagcactg ttccagccaa tcacaaaata
    13561 cagtgcagaa atccaagatc cagaaacaat ttcagaagtg attgccaatg cttatcgtat
    13621 ggctaagagt tcaaaaaaag gcgcgagctt tatcagtatc ccgcaagatg tagtggatgc
    13681 acctgtccaa gggaatgtca tcaagccatt gagcgatcct aaattaggtt ctgcttctgc
    13741 agatgatatc cgttacctag ctgaacgcat tagagaagca aaactaccgg tactattagt
    13801 aggtatgcga ggatcaagtg aaaaagaaac gttagccatc aggcaattag ttggaaagac
    13861 agcattgcct gttgtagaga cattccaagc agctggagtg atctcacgtg aattagaagc
    13921 gcatttcttt ggtcgtgtgg gtttatttag aaaccaacca ggagatatgc ttttgaaacg
    13981 aagtgatcta gttattgcta ttggctatga tccaatcgaa tatgaagcaa gaaactggaa
    14041 tgcggaaaaa gatgcgcgga tcattgtgat cgatgaagcg ccagctgaaa tcgatccttt
    14101 tatgcagcct gaaagagaat tgatcggtga tatctcagcc actcttgatt tattgacagg
    14161 ttcattagag cctcaacaag tgtcagaaga tgcaaaagaa tatttagcat ctcttcaagc
    14221 agaattaacg gagcgggaca ttgttcaatc aaaaggagaa gcaggtatct tgcatccttt
    14281 agaagttatt aacacgcttc agtcaaaagt aacagatgat atgactgtta ccgtagatgt
    14341 aggtagtcat tatatttgga tggcacgcca ctttagaagt tatgaacctc gtcatttgtt
    14401 attcagtaat ggtatgcaaa cgcttggtgt tgcattgcca tgggcgattt ctgctgcatt
    14461 ggtgcgtcca aatacacaaa tcgtatcagt ttccggcgat gggggatttt tattctctgc
    14521 ccaggattta gaaacggcgg tacgtaaaaa attgaatatc gttcatttga tttggaatga
    14581 tggacactac aatatggtag agttccaaga aaaaatgaaa tatcagcggg cgtcaggtgt
    14641 agactttggt ccagttgatt ttgtgaaata tgcagaagct tttggcgcta aaggaatccg
    14701 agctacaagt gtagaggaat tagaaaaagc tttagaagaa ggatttgcga cagaaggacc
    14761 cgtaattatt gatattccga ttgattaccg tgataatgag aaattgggag aaacaatctt
    14821 accagatcaa ttctattaag gaagtgtcga catgactgaa aaaatcttat accagcacgg
    14881 gacattagga gctttgatgg ctggcttgat ggatggaaca gaaacgattg ctcatatatt
    14941 agaaaaagga actctgggat tgggaacatt gcatgggctt gatggtgaag tcatcttttt
    15001 agatggcgta gcttatcaag gaagatcaga tggctcagtt gcccaattag atggaagtga
    15061 acttaccccc tatgcagcaa ttacagattt tacgccagat actagttttt ctgtctcaaa
    15121 aactgcagat ggagaacaat tgaaaaaaga catattagta agagaagccg gggaaaacct
    15181 atttttggca gtgaagataa ctggattatt caaaaatatg cacatccgta tcatgccgaa
    15241 gcaagaaaaa ccttacagaa gactagcaaa aatttcagag tctcaaccag aattccaaca
    15301 aagcaatgtc caaggaacat tagtcgggtt ctttacgcct gaattattcc aaggagttgc
    15361 tgcagcggga tttcacttgc attttatcga tgatacacat acatttggcg gacatgtaat
    15421 ggattttgaa gtggctgaag gaaaagttga gattagccgc atctcgtccc ttgtacagca
    15481 ttttccagtt gatgatccga cttttgttca cgaaacgatt gattatgctg atttagcagc
    15541 agaaatccaa caagcagaat agaaaaagac agcgagatgc gttcaaggtg attaacctga
    15601 cgtatctcgt ttttttgagg aggaaaccta gcgtttacaa aaaaactgag ctataatctt
    15661 ctccatagat caaaaaggag cggtttttat catgtggaca gaaaaacaga aaaaagatgc
    15721 atacgaaatt ttattattac agcaacaagg attattagaa gcagaagata actggttagc
    15781 aaatttagca aattcatctg cattgttgaa tgagactttg cctgagactg tctttgcagg
    15841 atattattta ttcgatggta atgaattgat tttaggaccg ttccaagggc gagtttcttg
    15901 cacacgtatc aagatgggaa aaggagtttg tggagaatca gctgaaaaac aagaaacatt
    15961 gatcgttgat gatgtcaaaa cccatgcaaa ttacatttcc tgcgattcag ccgcacaatc
    16021 agaaatcgta gtgccaatgg taaaagaagg taatttaatc ggtgttttag atatcgattg
    16081 cggagtaacg aaagggtatg atgaaatcga tcaagcattt ctagaaaaat atgtagatct
    16141 tttattagca aattttaata taaatgcttt ttcatttatt aataactaaa atcgtctatt
    16201 gaaacaaaaa agaagagaaa tcaagaataa aaagagccgg aaagacagtg ggcatttctg
    16261 tttttcggtt tttttatttt tttataaaat cacaagtgtt attgattttc ataatcatat
    16321 tttaatggta ttatcatttt aaataatgga aaaacattaa ggatggatga aatatgaata
    16381 acgaaactat cattttagga aaaaaatata tttgccaacc tattggcttg aaacaaaaag
    16441 tggttggaga agtaatcagt aaattagaaa attgtgttgt gatctgcgta gacgaatatc
    16501 atgacgtcga tcgcaatgaa atattagaaa aatgcggaaa agtagtggca aaatatactc
    16561 acgtttatga gttggaaaca acagaatatc tatataacta tagcaacaaa tatgaacata
    16621 ctttagtttc ataaagattt aaataaagtg ccttcaaaag cgagaagacc aagcttttga
    16681 aggcacttta tttagttggc agtgattgtt catgattttt gatttattca aaaggttcgc
    16741 ctagagaatc tgtctctgta acgaggcctg gtggagaaac tcgataaaaa cctatactgc
    16801 cagaactgtc ctattttcgg atagacaaac tagtagtttt aaaaagatca tccgatccaa
    16861 tcatttcaaa aaatgcaaaa ctgatatcag gatcatttag ctcaggcttg ttatcgtaaa
    16921 gcatttgtaa aggttgcatt tagttaggag taaacgcgaa actatcatca ttatttgctg
    16981 aagaagatga agaagctaat tcagagtttg tttgaaataa aggagacgtg ctgacagttt
    17041 ctttaccctg ttttttgcag aggtagaaac ttttatatcg gaagtttcta ccggagaatt
    17101 tttctcattt gtagaaattg ttgaatcgat actattcgaa atttgcgtat ctgaaggtat
    17161 gttctttgat gcagaagtcc cacaagcagt aagtaacaat aaatttgata aactgataaa
    17221 cagaattctt ttcattatgt acacactcct tatagtatcg tttagcatac acctatgctc
    17281 agtattaggc aaaatcaatg caaaactatc aagtctttta tagtatctat tagtcttagt
    17341 aatacatata tggagagggt gatatcccag ttcaaaccag atacattggt taccatctgt
    17401 attaacgatt atgcctctaa acgctttttt tatgaaaaaa tttataagtg aaaacgtagg
    17461 tatgatatta tctagaggta atttttttgg gtatttaaat agttgctagg aggatag
//
LOCUS       DACEUZ010000049        17180 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.049, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000049 DACEUZ010000000
VERSION     DACEUZ010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17180
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.049"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(543..2048)
                     /locus_tag="IXG44_001406"
     CDS             complement(543..2048)
                     /locus_tag="IXG44_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ1658132.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(2045..2731)
                     /locus_tag="IXG44_001407"
     CDS             complement(2045..2731)
                     /locus_tag="IXG44_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1658133.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="IXG44_001408"
     CDS             complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="IXG44_001408"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HAQ1658134.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
     gene            complement(4515..4694)
                     /gene="rpmF"
                     /locus_tag="IXG44_001409"
     CDS             complement(4515..4694)
                     /gene="rpmF"
                     /locus_tag="IXG44_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAQ1658135.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(4752..5321)
                     /locus_tag="IXG44_001410"
     CDS             complement(4752..5321)
                     /locus_tag="IXG44_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HAQ1658136.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            5507..6409
                     /locus_tag="IXG44_001411"
     CDS             5507..6409
                     /locus_tag="IXG44_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ1658137.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(6444..8228)
                     /gene="pyk"
                     /locus_tag="IXG44_001412"
     CDS             complement(6444..8228)
                     /gene="pyk"
                     /locus_tag="IXG44_001412"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HAQ1658138.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(8302..9264)
                     /gene="pfkA"
                     /locus_tag="IXG44_001413"
     CDS             complement(8302..9264)
                     /gene="pfkA"
                     /locus_tag="IXG44_001413"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HAQ1658139.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(9432..12746)
                     /gene="dnaE"
                     /locus_tag="IXG44_001414"
     CDS             complement(9432..12746)
                     /gene="dnaE"
                     /locus_tag="IXG44_001414"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HAQ1658140.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KRQLIYLLGEDNVVFK"
     gene            12977..13162
                     /locus_tag="IXG44_001415"
     CDS             12977..13162
                     /locus_tag="IXG44_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HAQ1658141.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(13224..14420)
                     /locus_tag="IXG44_001416"
     CDS             complement(13224..14420)
                     /locus_tag="IXG44_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ1658142.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(14590..16026)
                     /locus_tag="IXG44_001417"
     CDS             complement(14590..16026)
                     /locus_tag="IXG44_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAQ1658143.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            16319..17128
                     /locus_tag="IXG44_001418"
     CDS             16319..17128
                     /locus_tag="IXG44_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ1658144.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         5042 a         3595 c         2983 g         5560 t
ORIGIN      
        1 tctttttgtt ttaacaactc tttaatctta tcagattcag aagagaatgt caacaacttc
       61 tttgtttatt ttttctaaac gtctttgcca ctcattagca gcgacttgat tatcatacca
      121 tctttattgt tatttgtcaa taactttttc gaagtttttt tcgataaaat caagctttat
      181 tagctatctc atccaaacga cttcgttatt ataacatgac atcaaaagtg cgtcaacact
      241 attttttata atttttagtg acaccttaaa ggaatcattt agaacaaata ataatatagc
      301 aatctcttta taaaattgca atagaaaatt ccctattttt atttattatt tttaaacacg
      361 aatattacag cattttattg ttttttcgtt catctaaaag atacttacat ttaattaacg
      421 gatgttattc tatttaattg ttcgctagtt tttttataat gacatccctg aatttctcac
      481 ttgcttgtat tttgtacctt attctttgtt ggatatataa aaaaacaagt aaagcacatg
      541 ctttacttgt cttctgcctt tgcgatagga ataaagattc taaaaattgt cccttgccct
      601 actacacttt ctgctaagat acgtccttta tagttttcaa ccagttgttt agcgatagac
      661 aaccccagac catttccacc cttcgttcta gctcttgctt tatccactcg ataaaaacga
      721 ttgaagatct tattaaggtc ttctttgggt attccttcgc caaagtcctg aatcgcgatc
      781 tcaaattcat tcagagtgga agaaatagag atatggactt cttttcgtgt agttgaatac
      841 tttactgcgt tatccaatat gataatgatt aattgttcaa agtgattacg ataaatttgc
      901 agggtcactt cttttgtcaa atcatcatct aaagtgatca catagtctgg atataatatc
      961 ttgaaattat tataaacttg ataggtgact tcttttcctt tagttgtttc attcgcataa
     1021 tgaacatcta cttgttctgc tcttgataag tctagcatct cttgaactaa gcttttcata
     1081 cggctgattt cttgcatgct ggcgcttaat gattcttcta atatctcagg atcatctttc
     1141 ccccaacgat ttagcagact aagatgccct tcaataattg caacaggtgt ccttaactca
     1201 tgagatacgt cttccacgaa ttgctcttgt tgttcaatat acaaacgcat acggtcaagc
     1261 atctcattga atatttcagc taaatctgcc aattcatcat ttgtgtcgat ttcaggcatg
     1321 tggatatctg attgcggatc tttacgtata gtatccattg tatctctgag taccttgagt
     1381 ggttttaaga aataagaaga taaaaagaaa cctaaaatac tacttaagat caaagaaaca
     1441 acttctaaaa caataagagt caataatagc ttatttcgaa tatcatagaa agaagataat
     1501 tcatagaagc tttgcgcata gccgatcttt tctcttgtac ctttagaata tataggctgg
     1561 atagataaaa agccagtttt tccttctagt gtaacgatcg ttggaacttt acgtgtagtc
     1621 tgaatcaggt ttcgactgtt ttcatgggtt ttaaaaatca agttctcgtc caaatcataa
     1681 acataaagat ccattgatgg ctgccctagc tcagctataa aggaatcgat cttcattaag
     1741 tttccttcga ccagtgaagt atgatctacg acctcatctc cgtcatttgt cgctttcgtc
     1801 agattacgat acgtatttac taatgtcagt tcttcatccg agttagccaa acgagaagct
     1861 acttctgaaa tcgtacgctc tacattgttt ctttcttttg ccacaatcag attgatagat
     1921 gatttatagg taatgacagc gaaaattgta aacacaacga atatgaagaa agaactcgca
     1981 aaggcccact tgattgtcaa ggagggccct ttcagttctc ttttttccga ttttttcggc
     2041 tttatcacga gcgcatcaca taacctgttc cgcgtacagt ttggatatag ctttcctccc
     2101 cgggaacgtc aattttgttg cgaagataac gaatatatac gtccactacg tttgtttcaa
     2161 cttctgtttc gtatccccac actttattta gtaaaacgtc acgtgctaat accacgttca
     2221 cgttttccat taaagtaagc agcaactcgt attctctttt tgttagttca attacttcat
     2281 ttccgcgacg tacgacacgg ttttcttttt cgatagtcaa atcgcgataa gtaatagtag
     2341 tttgtttagc cacattttta tcgccttcaa tatcgattcg acgcaacaat gcacgaagac
     2401 gtgctaataa ttcttcgata gcaaatggtt tgacgatgta gtcatctgct ccgtggtcaa
     2461 gtccagatac acgatcgatc acagaatctc ttgcagtcat cataatgatt ggtgtatttt
     2521 tgacttggcg tacacgtctg cagacttcta aaccgttcaa ttctgggagc atcagatcca
     2581 acaatatggc atcccactca ttatttaatg ccgcatctaa accagttcgg ccgttgtagt
     2641 ggacttctgc gttataccct tcatgcttca actctagctc gacaaatcgt gccaagttct
     2701 tttcatcttc aatgattaga atattgctca tttattattt tatcctttct attctactgg
     2761 ctttttaaaa actctatcat tataccgata atatgcttaa aaatctatag tcattttatc
     2821 atggatagca attttaaaaa agagctgaag tctactcata acaatcgact tcagctcttt
     2881 cgtcctcttt ttattttctc atttcctaac agccgaaaga ctattattct tcatggtacc
     2941 atgagtaatg gaagattcct tctttgtcga cacgctcata ggtatgtgcg ccaaagtaat
     3001 cgcgttgggc ttggattata tttgcaggca gacgttctgc tctgtatgaa tcgaagtaag
     3061 cgatggctga tgagaaagtc ggtacaggta cgccagcttg gacagccaag gcaacgacgt
     3121 ctctgactga ttgctgatat tttttcgtga tttcaacgaa gtaatcatct agcaataaat
     3181 tttcgatctc aggatttttg tcataggcat ccgtaatttt ttgcaagaat cgagctcgga
     3241 tgatacatcc agcgcgccag atttttgcga tttcaccaaa tggcaagtcc caatcaaatt
     3301 cttttgaagc gacacgtaat tgagcaaatc cttgggcata actcatgatt ttactgaaat
     3361 acaacgcttc tcggattttt tcgaccaatt cttttttgtc tcccgtgaac tcaaaatcgt
     3421 tcgtacgaga caaaatcttg cttgcttgta cgcgttcttc tttataagca gaaatatagc
     3481 gggcaaatac agattcagtg atcaatggaa gtggcacacc taagtcaagc gcgctttggc
     3541 ttgtccattt acctgttcct ttattcccag ctgcatcaag gatcacatct actactggtt
     3601 tacctgttcc ttcatcgtct ttacgtgtca gaatgtcagc agtgatttct atcaaatagc
     3661 tgtctaattc gccttgattc cattctttaa agatatcagc catttcttct actgaaaggt
     3721 ttaaaatatt cttcattaag tcataagatt cagcaatcag ttgcatatct ccatactcaa
     3781 taccgttgtg taccattttc acataatgtc cggctccatt tgggccaata tatgtgacac
     3841 atggtgctcc gtcttctgct tttgcagaaa tttgctctaa gattggtgca actagcgcat
     3901 aggcttcttt ttgtccacca ggcatgattg aaggtccttt taaagctcct tcttcaccac
     3961 cggaaacgcc tgtaccgata aagttgatgc cagagttggc taactcttca ttacgacgca
     4021 tcgtatcttt aaagaatgta tttcctccgt caatcaaaat atcgccttta tcaaggtgag
     4081 gaagtaactc ttggattgtt gcatctgttg ccggtcctgc tttgaccatt agcaagatcc
     4141 gacgtggttt ctcgatagat tctacaaatt cttcgattgt atatgttgct ttgaaatttt
     4201 tatctgggtg ttcttctact acagcggttg ttttagcccc tgtacgattg aataaagcca
     4261 ctgaatagcc gcggctttct atgttcaagg ctagattttt acccataacg gccataccga
     4321 caacgccgat ttgttgtttt gacatatgaa ctcctcctac atacgggatt tctccctaat
     4381 ctcttacatt atagcttaac acggctacat tgcaaactaa agaacaacat attttttatt
     4441 taccgactac aaaaaaataa acctcaaggc tccatggtta gccttgaggt tttacatttt
     4501 ctaattaaaa tgtattaagc ttcttttgac attacgtcct taccatcata gtaaccacaa
     4561 tttgcacata cgtgatggct ttttttcatt tcaccacagt ttggacattc gtttaaccct
     4621 ttgattgtca atttgtagtg tgtacgacgt tttgcttttt tagcttttga tgttcttcta
     4681 gctggtactg ccattttgct acacctcctt gtaaagtctt gtatctaaat tagacacaat
     4741 atattccaat cttacttgtt atcgtcgtct tttgatttag aatcaaaaag ttcagatagt
     4801 tttgcaagac gaggatcaat tttattctcg gcttcttcca tttttctgcg ctggtattct
     4861 tcttcggaaa caacttccca atcattgcct tttggaagtt ctgttgctgc ttcctcttct
     4921 tttgtcaata cccgcattgg aatcgccaat aagatgttgt cttcaatcga ctctgctaaa
     4981 ttcaataatg gaccttcaat tagcaaaatc tcatctgcag acatttgttc atctctactt
     5041 tggaattgtt cttctgtcat gaatacttcg tctacagaaa aatccatggg cagttcaaca
     5101 ggttctagtg aacgtgttga aggaactgtc aaagtcgtct gcacagtgta atgaacgata
     5161 tagcctttct tatcaactga gaccaaacct tcgacttcca ctggcgcaac atcaagtatt
     5221 aaattgtctc gcttcatgag agcggctttc aagtcgagcg tctcatgaaa agttaacggt
     5281 gtctcctggt attttctcaa ttccaataaa gaccatttca tttttcatca ctcctaagca
     5341 acaaaagcaa ttatacaggg caaaaagcat gttgtcaatg aaattttctt gacaccttgc
     5401 agttcataaa aaaacagaaa aatctttatt agaaagcctc tgtttgctat catcctcaga
     5461 atcatttaca ataatcatta ttccattaaa ggagcggatg atcttcatga acacgaaaga
     5521 tatcgaatac tatgtccaaa tcgtcaaaca aaaaaatttt acgaaagtgg ctaattattt
     5581 ccgtgtgagc caacctacta ttacatatgc attacaacgt cttgaaaaag agtttgacac
     5641 cgtacttatt acaagagatc gatcgcatca cgaattgatc gtcacgcctt ctggaaagca
     5701 gctgtttctc catgcacaaa ccattctcca agagatttct actgcaagag ctgaaatcac
     5761 tcagttgcaa aagaaaaagc ttcgttttgg catgcctcct attatcggca atgaatactt
     5821 tcctaaactg tcaagttact ttatcaagca tgaattgatg aatcaaattg agattaccga
     5881 tggaggctct cgggacttat acgggatgat ccgacaaggg aggatcgatt tagctgtatt
     5941 aggctcaaca cagccaatcg tggatcaata tttagaaaca gagttacttt tggacaaacg
     6001 tttcatgatc gtcgtgccac ctacacatcc tctttccaag caggatactg tctctttcaa
     6061 agatatagca gatgaacagt ttgtactatt gaacgaacat tatgtccacc ccgcagcatt
     6121 cactaagctt tctaaacaaa cacatactca tccaaaagtt gtttatcaaa gcaatgactt
     6181 gacgatttta aagagtatgg tccgagaaaa tgtgggtatc ggttttttgg cagagattgc
     6241 tgtccatcct gaagatcatt tagtttgcct ccctctttca gatgaagttc agcctcgctt
     6301 cttgatttca ttggcaaaac gaacacagca gatcgtcacg cctcttcaag aacaagtagt
     6361 ccaagttatc catgaattcg ttacagcaca aaaaaacagc tcttcgtaaa agaagagctg
     6421 tttttatttt ttattaagct aaattagata gctgttgttt cgccacggta aacgatgccg
     6481 cgacgtgaat caacggtaat caattcacct tctgtaattg ttgttgttgc gcctttagca
     6541 ccaacgatta caggaatatc ttgtgcaatt cctacaactg cagcgtgaga agtcaatccg
     6601 ccttcttcaa cgataacagc agctgctttt tcgattgcag gcatatattc tttgtcagtt
     6661 gatggtacaa ctagaaccat tccgtctttt gcttttttta cagcttcttc agcagatgaa
     6721 gcaataactg catttgcaac gacagatttt tgtccaacgc cttgagcttc taataattta
     6781 gaaccaatca tttgaatttt catcacgtta gttgttccgc gttcaccaac tggtacacca
     6841 gcagtgatca agattaaatc gccttctgat gcgaaaccta attcaacagc ttttttagct
     6901 gctagttcaa acatttcatc agttgttgat ggtttttcag caactgttgg gtaaacgccc
     6961 cagtttaaca tcaaaccacg ttttgtgcgt tcgtcaaaag taacagccaa gatatctgca
     7021 tctggacgat attttgaaat catttttgca gtgtatcctg attcagttgc agcaacgatt
     7081 gttttcacgc ctagattttt tgcagcacga gctacagata aaccgattgt ttcagttaca
     7141 tctgttttat cgaattcgtt caattggaat gtacctaatt ctgataatgc attttcagct
     7201 tcttggtcga tacgtgccat tgttgaaaca gcttcaactg ggtagtcccc gtttgcagat
     7261 tcaccagata gcattgttgc atctgtgcca tcaaatacag cgttagcaac gtcagaagct
     7321 tccgcacgtg ttggacgtgg gttttcttgc attgattcta acatttgtgt agcagtgata
     7381 actgggatac cagcagcatt acattttttg ataatgcgtt tttgaaccat tggtactagt
     7441 tcagctggga tttcaacacc catatctccg cgggcaatca taacaccgtc agaaactttg
     7501 atgatttcgt caatgttgtc gataccttct tgagattcga ttttagggaa gatttgaaca
     7561 tgagtcatgt ctttttcttc taagatttcg cggatatcta gaacgtcttg tgctttacgt
     7621 acaaaacttg cagcgataaa gtcgatatcg ttgtctaaac caaaacgaat atcgtcagca
     7681 tcttttggag taatacctgg caaactaata gaaacgcctg gtgcatttac ccctttacgt
     7741 gatcctagca tacctgcatt tttaacttct acgactaatt cacggtttgc ttcgtctttt
     7801 tcaatgattt gcatatcgat caaaccatca tcaaataaaa tgtgtccgcc aacgtgaaca
     7861 tcgtcaaata atcctgggta agtaaccgcg attttttctt ttgaacctac atgttcagga
     7921 tccatagaga tgcgtgtttg atctcctact tcaaatttga tgtatcctgc acgaccaaaa
     7981 tcagcttctg ttgtgccttg aacagttgta cggatttcag ctccttttgt atctaaaaga
     8041 ataccgacat cttttcctgt ctttttaact gcttcacgga ccatagtcat acgtgataat
     8101 tgttcttcat ggtcaccatg agaaaaatta aagcggaaga catttgcccc agattcaatc
     8161 aattgagaaa taatttctac tgagttactt gctggtccta atgtactaac gattttggtt
     8221 tttttcattt aaaaaactct cccattcaat tttgtttatt taaagctgta cacagcttaa
     8281 atagcttggt ttctttcact cttaaaatga aagtgaatta tttagatcat atagtgataa
     8341 gtctggttta tgtttgttgt tttcaagtgt atcaatgatg tcagacgcaa cgatttcgtt
     8401 gtcatggata ccgacacata atccaccttt tccttcttga agcaattcga cagcgtaagc
     8461 accgaattta cttgccaaca cgcggtcgcg tgcgcttggt gctccaccac ggacaacgtg
     8521 tcccaagata gatacacgtg tatgatagtc accaaattct gaaagtttct cagcgaattc
     8581 atttccgccc attacacctt cagccaagat gatcaagcaa tgtttcttcc cgcgatcgcg
     8641 gccttcttgg atttttttag caactgaagc catatcaaaa tcatgttctg ggatgatgat
     8701 ttcatccgct ccgccagcca cacctgacca taaagcgatg tctcctgcat tgcgtcccat
     8761 tacttcgata acgaatgtac gaacatgaga agtcgctgtg tcacgaatac ggtcaattga
     8821 ttctagtact gtattgattg ctgtatcaaa accgattgtg aaatcagtcc caggaatatc
     8881 attatcgatt gttccaggaa tacctactgc tgggaaaccg cgttttgtca aagccattgc
     8941 gccatgataa gagccgtctc ctccgataac cactagacct tcgattccga attttttcaa
     9001 ttgttcgatt ccttttaatt gaccttcttc agttgcaaat tctggataac gtgcagaata
     9061 caagaatgta ccaccacgtt ggatcttgtc acctacatct gccacatcaa gacgacgaat
     9121 atctccagca actaaaccgg caaaaccata attgatcccg tatacttcca gtccttcgta
     9181 aataccttta cgaacaacgg cacgaacagc tgcattcatt ccaggtgcgt ctccgccact
     9241 ggtcaaaatt ccgatgcgtt tcataataaa cattcacctc attaaaatac tttatcctaa
     9301 gtatgactta ggtttacaac catttttgcg ttaaccacga ctaacattct aacattattt
     9361 ttctcacttg tcttgattta cggataaaaa aatgaaaaaa aatgaaagca agtagtattt
     9421 tttaccgtaa gttatttgaa aaccacatta tcttcgccaa gcagataaat aagttgccgc
     9481 tttatactct tgctgtcatc cacccaataa gcatctgaca gaactacttt tcgttgcgtt
     9541 ttttcgtgat acataactac tggtatatat cctgggtgtt tctgaaaaat cacttgtaat
     9601 tgtttcatta tctcgggatc ttcccgttct tctgtaatac gcaaatagca agtttgtgaa
     9661 ggatactggg cttctaactg gttaggatca gcaaagcttt ctccaattag ttgcagttcc
     9721 tgattatatt tgctgatttc gctccgccct tgaacaaaga tcactcgatc aacggcaaac
     9781 aaattacgat atttacgata aagagttgga aaaactgtga cagaaatttc tccactactg
     9841 tcggttcctt caacaaaggc catcagttct cctttttttg tccgaatttc tctaacttcc
     9901 ttgatcatca ctaataaatt gactggttga ttagggacga tatctgtaat cagctgaact
     9961 tgtttagcta aacgaagacg atcaaaccct tctgcaggat gacctgacaa ataaatacct
    10021 aagtactcct cttccagctt caagcgttct tctagtgtat agtcagcgac ttcttcttct
    10081 tttaatgcca tgattcccag caagtcaaga cttccaccac tgtaaacgat attttggatt
    10141 tttccttcga gatcaagcat caattgtttc ctgttaggag ataattcatc aaaaactcct
    10201 actgctgtca gtgattgcag taactctaat ttcagccaac gcttatcgat ccgtatgagg
    10261 aattgatcca ctgactggaa cgggccattt tgttttcttt cgtttaatat atcttctaca
    10321 aaatcacgcc gaataccttt tatcgctgtc agtccgaatc tgatttcttc cttattgacc
    10381 aactcaaaac tggaattact ttgattaatg gacggcgagg aaagtttgag tttattttta
    10441 cgtgcttctg caatatattc tttcaatttt gatgtgttat gccgaacaga attcatcagg
    10501 ctggtaaaga aagcacctgg atgatgggct tttagatacg ccatttggaa tcccacgaat
    10561 gaataagcaa atgcatgtga ccgattgaat ccatagtttg caaatttttc gatatattca
    10621 tatacggtcg ctgctgtttt ttccgaatat ccctgttgta atgctcctga tacaaaatgc
    10681 gacttttctt catctaatac gtcttttttc tttttactca ctgcccgtcg cagaatatct
    10741 gactgcccta atgtaaatcc agccatagtc gaggcgatct gcatgatttg ttcctgatag
    10801 acgatcactc catatgtgtt tttcaaaatc gggatcagtg aatcatctgg gtagcgaatg
    10861 gcttcttttc ctttcttcct ggcaatgaag gtatctatat tttgcatagg gcctggtcga
    10921 tacaatgcat tgactgcggc aatatcttcg atgttttctg gatctaatct tcgtaaaacg
    10981 ttccggatac cagcagattc aaattgaaat actcctgagg tttcgcctcg ttgaaacaag
    11041 agcagcgttt ttgcatcatt tagcgggatc tcttttaatc ggatctcata cccttctact
    11101 cgcttaacag ctttcaaagt aaaatcaata atcgataagt tgcgtagtcc taaaaagtcc
    11161 attttcaaaa gaccgattgc ttctacctca ttcatcgtat actgggtcag aaaagcttca
    11221 tcagaaccgc gttggagcgg aactagttca agtagattct tatcgctgat aactacacct
    11281 gctgcgtgag tcgaaacatg tcttggcaat ccttctaaaa tcactgcagt tttgaataaa
    11341 agttggtttt tttcgcttgc ctgaactaat tctactaatt gatgcgaagt tttataagct
    11401 tgggtcaatg tgattttcaa ttgattgggt attgcttttg accagcggtt tgcttcactt
    11461 tgcgataaac caaacactct agctgcatct ctaagaacca tttttgctgc cattgtgcca
    11521 aatgttgcga tttgtgccat atgatattgt ccatactttt ctcgaacata attcaatact
    11581 tcttcacgtc gattgtcagg aatatccaaa tcgatatctg gcatcgtaaa gcgttcgggg
    11641 tttaaaaagc gttcgaacag caaatcatat tctaatggat ccacatctgt gatttctaac
    11701 acataggcta ctaatgaacc cgcagcagaa ccacgacctg caccggtaac gattttgttt
    11761 ctatgagcaa aagccattac atcccatacg atcaagaaat aatcatcgaa tcccatttga
    11821 tggatgacag ataactcgta gtccaaacgt tcttggtatt cctttgtata gtcagtgatt
    11881 cggcgttcca cttgcgaata gcataactct cttaaatatt ctccagcttg ttttccatta
    11941 ggaactggat aatgcggaag taatttctga tggaactgga attcaaatga aatcgattca
    12001 gctaaacgct gggcgcctat tacagcttgg gaaagacccg ctttttcgaa tcgtttgatc
    12061 atttcttgtt ctgaaagaag atagacattt gctgttagtg gtaaagatgc agataaattt
    12121 tctaaaggta tttggcttcc ctctttgata tgtttcatca cttcaagtgc aaaaagttct
    12181 tcttgttgta aactagcgat gggatgtaat gcacaaagcg aataattttc ttttatcaca
    12241 tactgatacc attcaggcaa ttgatcaggc gtgttttctg tcaagccgat ataaaaattt
    12301 ctagtaaaaa cattttcaag taaaaactgc caatgaccga aaaagttatc ctctttattg
    12361 aaagcctcag aaacagaatg atttgccgga acaacaaacg ctagatggtc ggtttctgtc
    12421 atttctgcaa gttccaattt ttcttggatc atttttcggc tggaaagttt catcagctgc
    12481 tgataaccta tttcatcttt tgcgtacgca agcaagccat aatttttttc ttcataagta
    12541 taactaagat gtaatccaat gattggctgt actccttctt ctacacaaat acgatagaat
    12601 tcaggtgcac cgtataagac atctttatcc gttatggcta atgaagtgta gccttgtttt
    12661 tttgcttctt gtacatattc tcgaatacgt atcgtacttt gtaaaagaga ataagaagta
    12721 attgtataaa gttgtgggaa aaacatcttg cattccgcct tttttttcgt taaaattcac
    12781 tagcgttaaa aggtttgatc cttttacttt cattaagata gcaggcgctg aagttgatta
    12841 ttccacttca tttgcttgct tgtaatctgt aggaatgagt tctttcgcaa gaactcaaat
    12901 tttattgaga tttggtctcg tttctttaca ttcaaagaaa atctggtaaa attaagatag
    12961 aaaagaggtg ttctttatgc gatatatcgt tacattattc tgggctgtcg ttctaggtca
    13021 agtagtcggt tatattggtg ctgcattaac aagcggaaca tatgatttta ctttgacaac
    13081 aatcatttca ttcattgcgg gtgtcattat ccttttgatt ggtgcagttg cgccaagaaa
    13141 agaaacctct gcccattcgt aaaaaaacag ccaagagctt gagatttctc aagctctttt
    13201 ttattttcta ctcctcacca ccattataat ctgctgcgaa gaactttacg aaagttgaat
    13261 aaagaaagaa gaatatttga cagaacaaac aaacttgtca caataaaaac agcactgtaa
    13321 ttataagcat gcgcgactgt cgatcctagc atgggaccta aaacttgtcc aaagttgcta
    13381 aacatctgat tgtagctata gattcggctg actccttctt gaggcgtaat cttactgatc
    13441 agtgtattga cagaaggcat caaagcgcca gttgaaaagc ctaaaataaa gcgaagtata
    13501 cctaattgga gcggtgtagt gacgaatgcc ataggtaaat aacagcaaaa tgataaaatg
    13561 agtcctccaa ttagaacttt ttgattccca atcttatccc ctagttttcc tagtttcggt
    13621 gaagaaaaga cagcggaaac accagctatc gaaacaatca agccgctgac aaaaaggata
    13681 ttttctgtac ttccgctcaa ttcacgaata tataaggtca aaatagggct gatcgtagtg
    13741 attcctatct gtaaaatcaa ggtcgtaatg aagagaccga tcaaaataga gagatgatcc
    13801 atttttgaaa aaatttcttt ggtgcttatc agatcttttt tctcgattgg atggaaatct
    13861 tctttgacta agaaaatggt caataccgtt gtgatcatca acaaagcccc tgtgatcaag
    13921 aatacatttt ccataccaaa ccactgagcc agtgcaccgc ccattgaagg tccgatcaga
    13981 tttccagcaa tcgctccggt agacaatgtt cctaacgccc atccgctctt ttctctaggt
    14041 gcctgtgaag caatcatcgc tgtggcattt ggaatatatc cagacaaaat cccattccaa
    14101 aaccgcatga tcaaaagcca atacgcgttt ggtacaaaag ccaaagatcc cattgttaat
    14161 gtcatccctg ctgctgcacg gatcatcatg agttttcggc cttttcgatc agctagattt
    14221 ccccagattg gtgcaaccac ggcagatgca aaagcagtca cagagattgc tagtcccgaa
    14281 aacaattcga tttgatcttt tggtgttcct agttgctcaa cataaactgg tatgaatggc
    14341 ataactaaac taatacttgc accagtgaaa aaacagccaa tccacgcaat cactaaattt
    14401 tttctccaat taatctccat ctatcatccc tctttcgtct cttttatcat acctattttc
    14461 gaataaacaa gcaacgatct cgccagtaaa aagagaaaaa tcccgcaaag gtcattttat
    14521 atgtaacgtt ctttttatgt aaaacaaaaa gagatcagaa caaatactgt tccaatccct
    14581 tctctttagt taaattgttg ctagaatgat gaagttgata atgaataaaa tatcaacgat
    14641 ccacaaagca acagaaactt gattgtattt tcctttgatt actttcacaa ttgtatagaa
    14701 aataaatcct gctgcaatcc cataggaaat gctatagcac aatcccataa agatcgatgc
    14761 aaagaaggcc ggtaaagctt cttccatatc tagccatttg atgtctgcaa aagaagccat
    14821 catcattact ccaactaaaa tcaatgctgg cgcagttgct tgatttggta caatggcgat
    14881 cagtggagag aataaactac ttaatgcgaa tagcactgcc actactactg aagtcaaacc
    14941 agtacgtcca ccggccccga tcccagctgc agattcgaca taagttgttg tattcgatgt
    15001 accaaagata gcccccacag aagtagcgat cgcatcggca aatagtgctt tatccatttt
    15061 tgttttgaac ccacgaccat cttccaacgc gatctcgtct tcttttgaga aaatacctgt
    15121 gcggcgtcct gtaccgataa atgttccgat cgtatcgaaa gtatcagata aactgaatgc
    15181 gatgatcgtc atcaatactt gaggaatctt ggaagaatca ctgaataaag attgcatgcc
    15241 ttcagaacca aatgctgcac cgaaagttgt tcctaattct ttaaatgaat tacctaacga
    15301 attcgtttgc caatcaatgg aacttaaatc aacaacaccc ataaaaatac caaggactgt
    15361 ggtcgctacg ataccgataa gaatagcccc acggacattt tttacaacta aaatggctgt
    15421 cagtaccaaa ccaatcacag ccaataagat aggagcgttg tcaaagttgg ctaacgcagg
    15481 gacgattcca ccatttatcg taacatttgt cgcttttccg ccttcaacga cagagctcga
    15541 aatagctgac tggtctgctg aaaaagataa gaatcctgca tttttgattc cgacatacgc
    15601 aacgaaaata ccgatcccgc caccaatcgc atgttgaaga ctttcgggaa tcgcccgaat
    15661 aatcattttt cgaatatttg tgacagtaat aaaaatattg attaatccgc aaataaacac
    15721 cattgccaag gcttgttgcc aagaataacc aagcccaaaa acgaccgtaa aagtgaaaaa
    15781 tgcgttcaat cccataccag gtgcctgagc atatggtaca tttgcgaaca atcccattac
    15841 cagcgtacca ataatcgatg caatgattgt cgctagaaat actgcttgga agggcatacc
    15901 tgatgctgac aaaatcgtcg gatttacgaa taagatataa ctcatggcaa agaatgtcgt
    15961 tactcccgcc attatttcag ttgatacatt tgtattgttt ttctttaaac cgaaaaactt
    16021 ttccataaaa agttccactc cctgaatttt tttctgatgg aaaactataa taataaaaaa
    16081 taagtaaaat agggcaaatc catcgaacaa tttcaaatta tatagacgtt tcattcgctt
    16141 ttcaacgttt ttttaacaaa aaaagcgaaa ataatactta acgttcgtgt ttagcttatg
    16201 tttagctatt tatatagcca taactgtagt ttttgttagg aatagctgat tgctcttgtt
    16261 aattacctaa aatatgctag actaaaacaa atgctagttg aggaaggaag gacccttatt
    16321 gaataaaaaa ttaattgcca tcgatctgga cggaactacc ctcaatcaag attcgatgat
    16381 tacatctaaa acaaaagaga ctctgaaaaa agcaatcaat gccggtcatc atgtcagtat
    16441 tgctacagga cgcccattcc gaatgagcca tcagttttac caacaattag aattaacaac
    16501 accgatggtt aattttaacg gcgcattagt acacatccct aatcagcact gggatggtga
    16561 aagagaaacg ttgatcaatc gtgaaatcgc ttttgaaatc ttatcgcaaa aaaagcagct
    16621 aaatttagat tttatcgctg ctgaaaatcg tgatactttt tttattgaca gttttgattt
    16681 ctttgatgaa aaaatttttg cttcttctcg tccaggagag aagaatttgc tttctcctaa
    16741 aaatttgacg acaaatccta catctttgct tgtccgaaca gacaaacgtt ttgcaggagc
    16801 tgtatctgct gaattaacac gtcaatttgg cagttatgtc gatgttcgga catggggtgg
    16861 acctactgcc atcttagaga ttgtttcaaa aggaatccaa aaagcaaaag gcgtgcaaga
    16921 gattgctaac tacctgtcta tcgatcaaaa agacgtcatt gcctttggtg acgagcataa
    16981 tgatttggaa ttgttggatt atgcaggttg gggagtagct atggctaacg gtactgacca
    17041 actcaaaggc attgcaaatg atgtcactcc tttgagcaat caggaagacg gtttagctgt
    17101 ttatctggaa aaactactta aactataata gagaattaga ttccacaaaa aagaggttgt
    17161 gacaattttt gtcacaacct
//
LOCUS       DACEUZ010000050        17136 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.050, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000050 DACEUZ010000000
VERSION     DACEUZ010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17136
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.050"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(151..2058)
                     /locus_tag="IXG44_001419"
     CDS             complement(151..2058)
                     /locus_tag="IXG44_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HAQ1658145.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            2188..3063
                     /locus_tag="IXG44_001420"
     CDS             2188..3063
                     /locus_tag="IXG44_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HAQ1658146.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            3143..4117
                     /locus_tag="IXG44_001421"
     CDS             3143..4117
                     /locus_tag="IXG44_001421"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAQ1658147.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            4306..5442
                     /locus_tag="IXG44_001422"
     CDS             4306..5442
                     /locus_tag="IXG44_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAQ1658148.1"
                     /translation="MKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGRHAH
                     EKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIEHPA
                     VLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLMPIK
                     EIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKRDGV
                     NIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLILDTL
                     EANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNIEPS
                     HVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            5579..5923
                     /locus_tag="IXG44_001423"
     CDS             5579..5923
                     /locus_tag="IXG44_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HAQ1658149.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            6194..7480
                     /gene="efmA"
                     /locus_tag="IXG44_001424"
     CDS             6194..7480
                     /gene="efmA"
                     /locus_tag="IXG44_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HAQ1658150.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(7618..8217)
                     /locus_tag="IXG44_001425"
     CDS             complement(7618..8217)
                     /locus_tag="IXG44_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphatase"
                     /protein_id="HAQ1658151.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            8621..9742
                     /gene="mnmA"
                     /locus_tag="IXG44_001426"
     CDS             8621..9742
                     /gene="mnmA"
                     /locus_tag="IXG44_001426"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HAQ1658152.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNDKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            9988..11409
                     /gene="arcD"
                     /locus_tag="IXG44_001427"
     CDS             9988..11409
                     /gene="arcD"
                     /locus_tag="IXG44_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HAQ1658153.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            11423..12541
                     /locus_tag="IXG44_001428"
     CDS             11423..12541
                     /locus_tag="IXG44_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HAQ1658154.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(12601..13893)
                     /locus_tag="IXG44_001429"
     CDS             complement(12601..13893)
                     /locus_tag="IXG44_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ1658155.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            14043..14603
                     /locus_tag="IXG44_001430"
     CDS             14043..14603
                     /locus_tag="IXG44_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1658156.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            14732..15109
                     /locus_tag="IXG44_001431"
     CDS             14732..15109
                     /locus_tag="IXG44_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HAQ1658157.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            15042..17114
                     /locus_tag="IXG44_001432"
     CDS             15042..17114
                     /locus_tag="IXG44_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAQ1658158.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
BASE COUNT         5472 a         3105 c         3652 g         4907 t
ORIGIN      
        1 gaaactaagc agctttttca caacctctaa tacatggtat tccagaagta acttcttcga
       61 aaaataagcc gaaatgtcac aaaaatttga gaagcgattt tcccaaattc cttcttattt
      121 cttgaagctg accacttctg gcacaacctc ttaccagatt cgcactcttt tttctggcgc
      181 tagatacata gcgtcttgtt ccgtgatacc aaatgtttca tagaattcgt caaggttttt
      241 tggttgcacg tttgctcgga gtttagcagg ggcatggaca tcgatttgca gtaaaagttg
      301 ttggtattct ttttttgctt ttgtacgcca aatcgttgcc caattgacaa agaattcctc
      361 gatagaaaga tctgattccg tttttgctgc ttccaatgca cagcttagac cgcctgcatc
      421 agcaatattt tccgaaacgg ttaattttcc gtttactttt ccgtcagcaa aaggcagacc
      481 atcgaattct tcgatcattg cttgtgcttt gttttgaaat tgtgtaagat cttcttctgt
      541 ccaccagttg ttcaagttgc catattcgtc aaataaagcg ccgttattat caaatgcatg
      601 ggaaatctca tgagcgataa cagctccgat tccaccataa ttagcagagc ttgattgttc
      661 tagactgtaa aatggtgctt gaaggatcgc tgccggaaag acgatcacat tccgaaatgg
      721 atgataataa gcattgaccg tgtcagcact catttcccat tctgaacgat ccactggttt
      781 gttccatttg ctgaaacgat cttttaatgt gagtttgctg aaaacaagag tatttgaaag
      841 caaagtacca ccctgatctg caggtactgt tttgaactgg tcgaataatg ctgggatctt
      901 atcggggtag cctacttgga tacctaagtt atttaatttt atgacggctt tttcttttgt
      961 tgcatcccca agccagtcat tttcttctag acgtttttta taaacggcga tcattttttt
     1021 caccatttgt tctacgtctt ttttagcagc ttcgccaaaa tatttttttc cgtagtaatc
     1081 gccaactact tgatcgaatt gtccagaggc aaggtaatac gctgcttttt tctgaggcat
     1141 tgcttcgtct gttccagaca atacgcgaga atagattccg ctgacttggc ggaattcttc
     1201 tgatagataa ccactaagag aacgtactgt tttaatgatc atccagcttt tgaataacgg
     1261 aaaatgagct tctgtcagga tgtcattcaa atgatcgaag tacactggat cagttacgat
     1321 cactttatct ggagttgtcc caatcaaacc cgtgacaagt tttttcaagt ccagtttatc
     1381 tgtgtattta ataaatgtat caaaagattg cggattatac attttgctgt aatctgcgtt
     1441 ttcttctgca ctttttacat gaggagcaat caatttatca aattgcactg cttgttccgt
     1501 gattgcttct gcctgacttt tttctttgcc ggcaagctct aataactgaa cagccatatc
     1561 aaagaaaacg cctattagtt gtgcaccgtt gggatggtct gatgcataat acgtcttatc
     1621 tggaaggaat aaagaaggcg gataagcaaa gaaggcgttc gtttgcgcat tcttcatatc
     1681 tgcatctaca tccagattaa atggcaatgg taatgtatcc aacagccatt ctggaaattg
     1741 cttattcaat tcgtcgaaag actgcaatga ttcaactttt tcgattatag gttgaagcgg
     1801 ttgagcatgc aactcatttc ttcggtcata atcgtttgcc agttgataat atgcaaggaa
     1861 atgtttcatt ttttctgaag acactttttc tggatgggca agcagatcgt ctgtttctcc
     1921 catcaaaagt tggtcgatcc cgtcaactaa atcttggaaa ccgcctgtag ctggtttatc
     1981 tgcagggatt tttgctgttt tttgccattc ttcattgact gcttcataga aatcttgttt
     2041 caatcgttct ttgttcattc atcgatcgct ccttttttgt ttcgtgtgcc cattatctta
     2101 caaaatgaag gaattaacaa atagaacata aaaaattgaa aattcgaaca atcaaactga
     2161 tttgtttttt tcaatccttc acttataatg aaaaaaagga ggcgatccca actgaatcaa
     2221 gtagtggata aacctttata tttcaaagta gataaaaaaa ttaaaaaatt agccagcact
     2281 cagcagctgc agtcaagaaa aagtaaattg ctttttttag ctctggtttt tgaagatcaa
     2341 agttatgtga ttatcgatca atcaggacat ccggtagaat attctccagc aaaatatact
     2401 tatcaagagg gtctttcttg taaaaaatgg aagctgatca atgaagagcc aatcgagtta
     2461 tctagatgga tcaacagaaa agaagatatt cccgttttga tcgaagaaaa aaggtcagga
     2521 aaggaactcg caaattgctg ggtgggattg ccagaagaac gttttttgag gtataagaaa
     2581 tgggctaccc cctcaggcta tctttgtggt acttacgcgg cagcagttct ccttgcctat
     2641 tatcaagact atcgtaaggg gtggatgctt ccaaatgaga ttcgcaagaa aaatacggct
     2701 gattcgttgg ttttgacgaa ggcattgcgt agccagatcc aaccactagg acttcctacg
     2761 attccttttc aggttagtac agggatttct agttttttaa agaagaatgg caatcatgag
     2821 cgtgcacgag caactcttct aggttcatgg cagcgagcta caaaacgtat acgtgaaggc
     2881 aaacctgtga tgattggtat tttgaaagta cttggcagta catatggcaa tcattgggtg
     2941 acagcgtacg cttattttga aactgaaaca ggagaacgtt attacaaagt acacgacaac
     3001 tggggcgatt atcataaagt cattcctgcc agttggagta acggaacagt ttcacttcct
     3061 taagaatttg ctgaaattaa cagaaaatct tggaaaaata tgttaaacta tcgaagaata
     3121 gagaagtttt ggggaggact acatgagtaa taagtatcag gatgacacgt tgaagatttt
     3181 tagtttgaat ggcaaccgtc cattagctga aaaaatcgcg aaagtgttcg gaacagaatt
     3241 aggtaaaagc gtcgtgaagc aatttagtga tggcgagatt tccatcaata tcgaagaaag
     3301 tattcgaggg gatcatgtgt atatcgttca atcaacgaat gcacctgtca atgattacta
     3361 tatggaacta ctgatcatga tcgatgcaat gaagcgagca agtgcgaaaa cgatcaatgt
     3421 cgtattgcct tactatgggt atgctaggca agaccgcacg gcgaaacctc atgaaccgat
     3481 cactgcgaaa ttgatcgcta acttgatcga agaagcaggg gcaactcgag tactgacttt
     3541 agatctgcac acggttcaag tacaaggatt ctttgatatt cctgtagata atttatttac
     3601 gatgccgttg tttgctcatt attaccgtca attaggtctg acgggagatg acatcgtagt
     3661 tgtttcgcca aaaaacagcg gtgttcagcg tgcacgtagt ctttcagaat atttgaacag
     3721 cacattagcc atcgtagatc atgcagatga tgcttcaact gagggtggtt atgtgatcgg
     3781 tgatgttcaa ggaaagacat gtatcatggt agatgatatc ttgaacacag gagcaacgct
     3841 tgcacgagca gcaaatgtat taaaagaaaa cggcgcaaaa gaagtatatg cctgtgcgtc
     3901 acatggatta ttgtccgatc atgcgaaaag tattttagac aatgcaccaa tcaaagatat
     3961 ttgtatcact gattctgttt tcacagaaga aaatcgtcat ccagaaaact taacaatcgt
     4021 tacatgttca gaattaatgg gtgaagcggt caaacgcatc catgaaaata caccgatgag
     4081 tccgttattc cgtctggaag aaaaagaatt cgaataaaca aaaaaaagag ggaaagaagt
     4141 tgtgacagaa gtgagcagct tcaaggagct gcgacaagac ttttgtcaca gctccttttt
     4201 tctatgccca tgaaacgtcc tctagacaaa aatgagctct tctttggttt gttcgctatt
     4261 ttatgctaga atgtgcctag caataatttg gaagggatga atgaagtgaa aactatttat
     4321 ctggatcatg cggcaactac ccccatgcat ccacaagtaa ttgaagcgat gacggcaatc
     4381 atgcaagaca cgtttggaaa tccttcaagc atacatggat ttggtcgcca cgcgcatgaa
     4441 aaattggaaa cagcaagaca aatcattgca gacagtctgc aagcaaaacc tcacgagatc
     4501 atctttaaca gtgggggaac agaaggggat aatacagcta tcttagaaac agcgttctcg
     4561 cgtcaaaaag aaggcagaca tctaatcact acagctatcg aacatcctgc tgttcttaat
     4621 accatgaaat atttagagac aaaggggttt gacgttacat atctgcctgt agatgaaaaa
     4681 gggaatctgt cgatcgaaga ggtcaaaaaa gcgttacgag aagatacgat cttggtctct
     4741 gtgatgtacg ggaacaatga aatcggaaat ttgatgccaa tcaaagaaat cggtgaactt
     4801 ttagcggatc atccagctta tttccataca gatgcggtac aagcgtacgg aaaccaaacg
     4861 atccatccag aagaattagg aatcgattta ttaagtatct ctgctcataa aatcaatggg
     4921 ccaaaaggcg tcggtttctt atataaacga gacggagtaa atatccctgc attgctttta
     4981 ggcggggagc aggaagaaaa gcggcgtgca ggtacagaaa atttagcagg gatctggggt
     5041 atggcaaaag cagttgaact gctgtcacct gaagaacgtg aagcacgtaa caacaaatat
     5101 aaaggattcc agcaattgat tcttgataca ttagaagcaa acgaaatccc ttatcaagta
     5161 aatggtgatt taacgaataa actccctcat gtgttgaatc ttcgcttaat tggcgaagca
     5221 aatgatttgc tactgatgaa gcttgatctt aatggggtcg ctatttctac tggttcagct
     5281 tgtacagcag gaaatatcga gccttcacac gtattagaag caatgtatgg agaagataca
     5341 ccgatactaa aagaatccat ccgtatcagc tttggtttgg gaaataccga agacgacatc
     5401 cagtatttcc ttgatcaatt agttcgtgct gtcaaaaaat aactagtgaa gtcattctta
     5461 tatgaacaga tgtctgagag aacgccttca ttttttaggg aacttaactg aaaaacctag
     5521 taaaaatcag gataaacttt tataataaag agaaactaat tttatcgagg tgaaaatcat
     5581 ggcatttgaa acaacagcaa cggtactggg cgcaccaatt agctatcgtt tgcatcctga
     5641 tgcaaaaaga tatacattgc gcgataatgg atttacagaa acaaacggag gcaattatca
     5701 attgatccga ccattagatg ccacgccgca aagcaaagaa ggattcaaat taaaaatcac
     5761 tgtatcaaaa gatattcaaa cattgaaaat gtcgatcact acagcaaatg gattgaaagc
     5821 agtcaatatt ttcaaagatc caaaacagaa aatgagccaa gataaatttt attttctgat
     5881 ggatgggatg attagccgtg ggttattcga aaaagtcgaa tgatctgtgg atataaaaag
     5941 acaactagtt ttgaaacggc ctattgttag atgactaact ttgtaaggac gacgcaaatt
     6001 agttgtcttt tttattttta aatttaacac atgttgttat ttttaatatt ttgctgattt
     6061 attgtttcta agtaaagaaa tttaaattga aacgattgtt atttagggaa aaaggtattt
     6121 tttgaaaaaa gaatcccgta taatgattgt tgtgcaaacg ctatacaaaa tgaaaaagaa
     6181 aagagggatg tttatggaaa atgaacagtc ggtcgtttta acgaattgga agagaaatta
     6241 tttgttcttt ttatcgggac agtttttgtc aggaatcact agtatggtcg tccaatatgc
     6301 gatcatctgg tatttgacaa gagaaaccgg ttcggcgaca attttgagtt ttgctacttt
     6361 actaggaatg attcccatgg ttttactaag cccatttgta ggaccgctag tggatcgctg
     6421 ggataaaaaa gctcttttga ttgtcacgga tattattgta gcgattttcg cgcttatttt
     6481 agcagtagtc ggaacgattt cagaatcctt cccgatttgg ctagtttttg tgtctttgtt
     6541 tatgcgttcg gtcgcacaaa cattccaaat gccaacgatc cagtcgatta tgccgacaat
     6601 cgttccatct tctcacataa caaggacgaa tggacagtta ggaatggtcc aatcagcgaa
     6661 tttcatcatt gcgccagctc taggtgcggc tttgttttca gtcgttcctg tcaattacct
     6721 tattttatta gatgtattgg gggcagtttt tggtgtcggc ttattgattt ttgtgaagat
     6781 cccaaaagtt tctcctgaaa tattggaagt tccgcttact atttttaaag atgcaaaatt
     6841 tgggctgcag caattgatgg ataataaagg gttatggtac ataacgatca atggtgcatt
     6901 tgtgatgctg ttgttcatgc cagcaatcag tctgtatcca ttgatgacgc tagattactt
     6961 tggtggttcc gttggacagg cgggagcagt ggaagtagtc tacgcagtcg gaatgctctt
     7021 aggcggtgca ctgatcagtt ttataggaac atggaaagac cgaatgaaac ctattattat
     7081 agcctacatc attatgggcc tgacgatcgg tgcaagcgga ttggttccaa acgatagtca
     7141 agggttcttg tactttctta tcttgaatgc aggagcaggt tgtgcaacgc catatttcaa
     7201 tacactgctg atggcaatga tccagcagag ttatgaatcg aatgttttag gtcgtgtctt
     7261 agggaacttc aactctttga tgaacctggc aggtccaatt ggattattat ttgcaggacc
     7321 attagctgat cgtttagggg tagaaaaaat gttcttgttt tccggaatcg ggattttatt
     7381 atgcggaatc gttttatttt taacgtcagc tgcccgaaat tatgataaag aattacagaa
     7441 aaaactggta aaagaacacc atgagcaaaa agacgaatag tatggaatgg agttgtgaca
     7501 gaagtcttgt tacaactcca tttctgatcc taatgttcct gtggcaataa gcctactcaa
     7561 atgaaaaata tgagtaatta tctttatttg tttaatactt gatatagggc agttttttca
     7621 cgacctatcg tacttcctct ttgcttcata gatttctttg atttgttgct cagataacgg
     7681 agtgccaaat ggtagttttt cctttaagag ttctctttcc ggtgcgtcta cgcctacatt
     7741 gacatcgttc tcgataggta cgctgtaatg atggatatgt ccatgaagat tgcggatagt
     7801 gtcgttaggc ccgagcaaca tcggataatg agtcaagtaa tattgttgat ggtcgaattt
     7861 aacgatggca cctacatcat aaaaataaaa tttgggactt ccgtcagtaa gcctaggatc
     7921 atttttttct aaataacgga aaaaagcgcg actgtcatgg ttccctttga tcaagtggat
     7981 tgttccttgc agttgagtga ccagttccaa aatttctgca tttgcttttt catattgcgg
     8041 atgcatagca aggtcgccta gatgataaac atggtcggtt tcttttatta ctgcattcca
     8101 gctagtgatc aactgttggt tcatttcatc cacatttgca aagagccgcg gagcaaagtc
     8161 gttttttcct aataagcgtt catggaaaaa gtgcatatca ctgataaagt aattcatgta
     8221 tcttttgcct cccttttttc ttagagtaaa caaaatcact tatctgagca actcataaca
     8281 tctaaaaaga aaaggttgta aatgagacgt gcagctttaa gcgataagaa gcaattttga
     8341 aaaatagttt ttcacaattt ataaaattat tccttattgc cgaaaggctg gcccttttta
     8401 tgagtatgcc aattttttat atcgaatgct caaaagatga gtgatttttt ggttgactat
     8461 catgtttgtg tactgaatag gtccagtaga acagtatttt tacgacttga taattaaggg
     8521 atgaaatttt cagaaatatg acgtataatg taaaatactg aaaagttgcg accttcaact
     8581 cgtagatgtt tcagaatcta attgaaatga tggtgatatc atgacagaca acagcaaaac
     8641 acgcgttgtc gtagggatga gcggcggtgt cgactcatct gtaacagcac ttttgttaaa
     8701 ggaacaagga tacgacgtga tcggaatatt catgaaaaat tgggatgaca cggacgaaaa
     8761 cggtgtatgc acggccacgg aagattataa ggatgtagcg aaagtagctg ctcaaatcgg
     8821 gattccttat tactctgtga attttgaaaa agaatattgg gatcgtgtat ttgaatattt
     8881 cctagccgaa taccgcgcag gacgtacacc taacccagac gtgatgtgca acaaagaaat
     8941 caaattcaaa gcattcttgg attatgcaat ggatcttggc gccgattatg tagctactgg
     9001 acattatgcg caagtcactc gcgatgaaaa cggcgtggtc cacatgcttc gtggagtaga
     9061 caatgataaa gatcaaactt acttccttag ccaattatca caagaacagc tttcaaaaac
     9121 gatgttccct ctaggcggca tggaaaaatc agaagtacgg gcaattgctg aacgcgcagg
     9181 ccttgcgact gctaagaaaa aagattcaac gggaatctgc tttattggtg aaaagaactt
     9241 caaacaattt ttgagcaact atctgccagc aaaaaaagga aatatggtca ctcttgatgg
     9301 tgaagtcaaa ggacaacatg ctggcttgat gtattatacg attggtcaac gtcaaggact
     9361 agggatcggt ggcggaggag attcgcaaga accctggttt gttgtaggaa aagatctagc
     9421 gacaaacaca ttatatgtgg ggcaagggtt ccatcatcct gcattgtatg ccacaagctt
     9481 ggatgcaagt gaaatccact ttacgacgaa tgaaccaatg ccaaaagaat tcaagtgtac
     9541 agcgaaattc cgttatcgtc agcaagacgt tcctgtgaca gttcgcttat tggatgataa
     9601 tcgtgcagaa gtcgtattcg acgaacctgt acgtgcaatc actcctggac aagcagttgt
     9661 attttatgat ggaatggaat gtctgggtgg tggtttgatc gaccacgcat atcaagaaac
     9721 aaaagtttta caatacgttt aagcgatatt aaatgaatca aaacagctat ggctaacaaa
     9781 agaaacacct tttcttttgg atagcatagc tgtttttttg caataaaatt aatttttatt
     9841 cattttatac ttgaaatact tgtgtaaaaa gttcataata tccaataata tgaataaagt
     9901 tgctttttgt taagcaagag cagttgcaaa atagaaggga atcttttgca agtgatttgt
     9961 tcaattttat aagtgagggt gatggaaatg gaaaagaaat cgacgggtat tagcaaatgg
    10021 ggactggttg ctctggttgt cagttcgtca atcggaagcg gtgtgtttgg gatcacaagt
    10081 gatctggcta gttctgcagc tccagggcca gcgatccttt catggataat cgttgggatt
    10141 ggtattttag ctttggtatt atcgctgaat catttaggag aaaagcgtcc agatctagat
    10201 ggtggtattt tcggttacgc ggaagcttca tttggtaaat taggtggctt catcagtggt
    10261 tggggttact ggttgtctgc gtggctggga aatgttgctt ttgcaacgat gctgatgagc
    10321 gccatgggag aatttttccc gatattcaaa ggtggacaaa atgttccttc tattttattt
    10381 gcaagtattt ttatttgggg tctgacttta ttagtaaaca atggagttga aggagcgagc
    10441 gtcatcaata caatcgtgac gatctgtaaa ttagtgccat tattcctatt tttaatattt
    10501 gtatttatcg catttaaagt gaatatattc actgctgatt tttggggtaa cgtatctgat
    10561 aatctagtga atggaacagg gcagcaagga acgatctggt tgcagattaa aggttgtttg
    10621 atggttatga tgtgggtctt tgtagggatc gaaggcgcaa gtgttttagc caaccgcgcg
    10681 aagaaacgtt cagaagccca gcaagcatcg atcattggtc tgctttgttt attgtttatc
    10741 tatatccttg cttcgatctt gccatacggc gtcttatctc aagaagagtt agcaactatt
    10801 tcacaacctg ctatggcaaa tattttaaaa ggaatcgtgg gaaattgggg agcagcgctg
    10861 atcaatatcg gcttgattat ttcgatcatt ggttcttggt tgtcttggac gatgctccca
    10921 gcagaaacaa cgatgttgat ggctcgagac ggtctgttgc cgaaaggttg gggaaaaacg
    10981 aataaaaaaa atgccccaac ttattcgtta gttgttactg caatcttgac taatctattc
    11041 ttattgactt tcttagttac tgattatgcc tatcaatttg cctattcttt atgtacagcg
    11101 gcaattttga tttgttatct acttgtcggg atctatcaag tccagttttc tttaaaagaa
    11161 ggaatcaaaa aacaagtagt gatcggtggg attgctgtag tttttgaatt gatgggcatc
    11221 attttagcag gattttctta tgtcctttta tgtagtattg cctatcttcc tggtttttgc
    11281 ttctactgga tcgcttgtcg tgaaaccaaa catcaaatta cgggaaaaga aaaagtaatg
    11341 attgggctga tttgtgtggc cgcactgctt tcaatcgggt tgcttgctgt aggctggatc
    11401 aagatataag ggggaaagaa acatgaaaat cgcaaatttc ggtgtagaag aatggctaaa
    11461 taaatgggaa aagaaagcgg tatatgatat tgcacaaagc tcgatcgaag cattgacatt
    11521 agacgaactc gttggcttag atgggactag cgtaagtaaa tttttcgaaa aacgcaaaaa
    11581 cgaaccattg gactatggct ggatcgaagg atcagatgtt ttcaaacaag aagtcgcttc
    11641 tctttatcaa accgtcgatc cggaaaacat cttacaaacg aatggagcga ctggtgctaa
    11701 tcttttagct ctatacgcgt tagtggaagc aggagaccat gttgtttcga tgcttcctac
    11761 ttatcagcaa ttatatgaca tccccaaatc tttaggtgcc gctgttgact ttgttcattt
    11821 gaaggaagaa gaagattggc agtttgattt agaacagcta gaagccttag tcaaacctga
    11881 aacaaaaatc atctgtttga atagtgcaaa caatcctaca ggcacattgc tagatcggaa
    11941 aactttagaa aaaatcgcag aaattgctcg tacagtagat gcatatgtat tgatagatga
    12001 agtatatgcg cctttaacgg ataaaggtga attcttgtcg atcgtagatg tctatgaaaa
    12061 aggtatagca accaattccc tatccaaaac ctactccatt ccaggtattc gtattggctg
    12121 gacagctact ggacctgagt tggcagaagt ttttcgaaag tatcgagact acacgatgat
    12181 ttgcggcgga gtcctgtcag atgatcttgc tgtacatgca ttgaagaata aagaaaaaat
    12241 attggcgcga aacaaaaaga tcattacaga gaaccttgtg attttaaaac aatgggtagc
    12301 caatgaaccg aaagttgaac ttgtcgctcc caattacgtc tctacttctt tcattaaatt
    12361 agccatcgaa gaagatgacc agacattttg tatcaattta ttagaagaaa caggagtact
    12421 attagtacca ggaagtgcct ttgatttacc gaagcatgcc cgactaggtt actgttgtaa
    12481 aaaagaaacc ttggaaaagg gattattcct cttatctgag ttcttgaaaa aacaggccta
    12541 aacaaaaaca ctgcaaggac agtctgtgac aactgttcaa gcagtgtttt tcatttacgc
    12601 ttattgtcta tgagaaggag agtgccctga agctatggag actcctgaag ctttttcttc
    12661 tgcagtttct tgctgatgga tctcttgtgt attgtgtcgt acaagcaaga agttgaccaa
    12721 aacaagaaga gaagtcgaaa gaaagacgcc acgataacca aatgcactgg aaacactgga
    12781 gccgatcata ggccctacta cgttaccagt tgcttggaaa gattggttat aactgaagat
    12841 ccgtccagca gcttgatgag gcgaatattt tgtgatcaat gcttggacag cagggagcag
    12901 acaagcatca gagataccaa tcaaaaaacg taaggcagct aattgccaaa cattcgtgac
    12961 gaatgccatt gggatataaa ccagaatcgc aaaacccaaa ccaatcgcaa gtattctttc
    13021 actcccaatc ttgtcaccta agcgaccaaa tcttggagcg gctatcaaag tggcaatccc
    13081 aggtatcgaa gcaatgattc cgctgatcaa cgtaacattc ccatgtccat gcagcaattg
    13141 gcgaatgtac agactgatga ttggactaat ggaattgttg gatgcttgga tgatcatcgt
    13201 tgtgacgaac ataccgatga cgacgtgtgg atatttcaag tctttgaaaa tctgtttggc
    13261 agaaaccatg ttttcttttt tcactgggac aaatttttca tggacgaaaa acagactcaa
    13321 caagaagacg aaaaataaga tgatccctgt aatgaaaaag gtagggcgat agccaaacgc
    13381 tgaagcagag atcccgccaa gaagcggtcc aagtaatgtt cctgtaacag aaccagttgc
    13441 taaggttcca agaacttgac cacttttttc ttttggcgtc ccagttgcga caagggcagt
    13501 ggcattactg atataaccag aaaaaatccc ttgaagcaga cgcaatgcga ctaattgata
    13561 cacactagtc acgcaaccca tcaaagaaat gacgattgcc attcctaatg aagcacgcaa
    13621 cagcatcaat tttcttcctt tttggtcggc taattttccc caccaaggag agataagcgt
    13681 agtcaccaag aaagtagatg aaaaagtcaa tccgctccaa aagttcaatt gtgacgtact
    13741 ataattacct aacgtatcta tgtaaagtga cataaaaggc atgactaaac taaatccgat
    13801 accagccata aatgttccaa accataagac aaacaagttt tgttcccaag tttctctttt
    13861 cctaaataat ttttccttta ttcttgcagc caattagact ccttctttct gaaaattttc
    13921 atctacagct ctttattata aactacaata tgtagtttat caatgaaagt ctcttttttc
    13981 tctttctttt tcgtggtaaa ctgaaagagt gaaggattta ttttatgaga ggaaacatgg
    14041 aaatgaaaaa aagaaatctg tctcaagaga aaatcatcga ctgctttcga gaactagcag
    14101 aagagatgga tgttcagcaa ataacctttc agcatttagc caaacgcttg gatatcaagg
    14161 ctccctcgct gtataaccat ttcaaaaata ttcgtgatgt caaaacagcg ctaacagcga
    14221 aacttctgca ggaacttaat gaccagctgc gacgggctct tgtgggaaaa agtggtcagg
    14281 aagcactgca gatctatgca gaaacttatc gatcctttgc tttttcaaat caagcagtgt
    14341 atgagttact gatcagtgta ccgcatacca atgaagctgt tttattagat ggaatccatg
    14401 aaacgaatca gatcattttg cagatatttg aggcttttca attaacgaaa gaagaaaaag
    14461 ttcatcgaag cagggaactt cgtagtatga tccacggtta tctttcctta cgttttttag
    14521 gatactttac aaaagaacca acaatcgatc cagaagacag ctatttttgg atgatcaata
    14581 actttatcgc tactttgcct taaacaatca gattcatttg taacaatttt aagatgttca
    14641 ttaaatgacc cttttataag agaaaattct tgcaactttc ttgaaaagtg actaagatga
    14701 gtctagaaaa gctgaaagaa ggagcacgag tatgtatcaa gtaattacca tgttcgggga
    14761 caatgagcct tggtggtttt ttgaagactg ggaagaagat attgaagaag aagaaacatt
    14821 cgagtctttc gaagaagcac gacaagcgta cgaaaaacaa tggaacgaaa tccatcaaaa
    14881 ttatgaatat atcaatgcaa aaagtaactt tctcagcgcc ttttggaatg acggagatga
    14941 gcgctggtgt gaagagtgtg acgatgattt acagcaatac aagggacttg cgttgttgaa
    15001 aaatcatcag cccatcacag tagaaagcag aaaggaattt tatgaaacaa ctaattctag
    15061 cggaaaaacc aagcgttgcg aaagacctaa gcaaggtgct tggtgctaat caaaagcaca
    15121 aaaattatta tgaaggaccc aaggtgatcg ttacttgggc ccttggtcat ttattaggat
    15181 tgaaaatgcc ggaagatctg aataaggaat ggcagagctg gcaaatggac acgttgccaa
    15241 tgatccctaa aaaactagga atcaaaccac ttcctaaaac aggacatcaa ttaaaagcaa
    15301 tcaagcagct ggctcagcga aaagatgtct cagaagcagt cattgcgaca gatgccggaa
    15361 gagaagggga acttgttgct cgctggatct tagagtatgt gcatttcaat aaaccagtga
    15421 agcggttatg gatctcttct caaacagata aagcaatcaa aactggattc aagcaactaa
    15481 gacctgcgaa agaatatgat gcattgtatg aatctgctct tgcccgggca aaagcggact
    15541 ggctggtagg attgaatgtg accagagcct taactgtgaa gtacgcggat aatttatcag
    15601 caggccgtgt gcagacaccg acactagcat tagtgagaga ccaagaaaag aaaatcgagc
    15661 agttccgtcc acaaacgtat ttcacgatca cgttgcaagc tggaaaagaa caagccaaaa
    15721 tgcttcaaaa aaatcaattt gctttaaaaa cacaagaaga agcacaaaaa cttgttcaat
    15781 tgatcagtac aagcaaagga actgtcacag aagtccaaga aaaattaaaa acggaatctg
    15841 cgccattgcc ttatgattta acagagatcc aaagagaagc caatcaacgc tatggctatt
    15901 ctgcaaagaa aacattaagt cttgtacaaa gtttgtatga gacccataag atcgttactt
    15961 atccgcgtac agacagcaaa tatctgacaa atgacatgaa aagtacgatg aaagaacgat
    16021 tgcaagcagt cagcgacttt gctcctgagg tcaagcagta tttgaaaaac ggagccatcg
    16081 tccaacagac aaaagtattc caagacagta aagtgaccga tcaccatgca cttttgccaa
    16141 ctgaaaacag agcacgttat gaaaaattaa gtaatgaaga gcgaaaaatc tatcaaatga
    16201 ttgtctctcg ttttttagga ctgtttgctc aaccccataa agtcagccaa acaaaagtta
    16261 cggttgaatt tggtaaagaa cgattcattt ttcggcaaaa tcgtgtcgtc caagcaggat
    16321 ggaaaggcga aactgaaact gagacggaaa cagtcaaatg ggaaaaagga atgagaatca
    16381 atcccgattt cgccatcaaa aaagaactat ctgcaccgcc taaaccattg tcagaagcaa
    16441 gcttgcttgg tcaaatggaa aaatacagtc tgggaacccc agctacgcgt gcagagatta
    16501 tcgagaaact aatcaaatct gaactgatgg aacgtacccc tcaaggactg caagtatctc
    16561 ctaaaggtaa gcaattatta gtattggtaa atccctcttt agtaacacca gatttgacag
    16621 aaagctggga aaaagacctt gaagctattg ctgcaggaaa gaaacaagca ggaaccttct
    16681 tgaatggaat cgaaaaagaa acaaaacgct tggtgaatga gatcaaatca agcaaacaag
    16741 aatatcaaga tttctcgatt acccaaaaga aatgcccaga atgtggggcc aatctgcgcg
    16801 agaaaaacac acgtgacggc aaaatctatg tctgcaccaa ttcagattgc aactaccgca
    16861 gaagaaaaga tccaaaagtc tctaatcatc gatgcccgca atgtcaccgg aaaatggaaa
    16921 tcatcgaagg taaaaacggt gcttatttcc gttgtaaata cgatggaacg acagaaaaat
    16981 tgcttgataa aaaagagcag aagaagaaaa tgaccaaaca tgaagaacga cgcttgctga
    17041 aaaaatatgc acaagcagaa gaaccagaag aaagcccatt agcagcagca ctaaaagcag
    17101 caatgaagaa gtaataagat atgaaagagg ttgtga
//
LOCUS       DACEUZ010000051        17108 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.051, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000051 DACEUZ010000000
VERSION     DACEUZ010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17108
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.051"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            <1..379
                     /locus_tag="IXG44_001433"
     CDS             <1..379
                     /locus_tag="IXG44_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ1658159.1"
                     /translation="EVDPEEKPDVTPEPDTDARDQGIPEKINKKTIQEDGKKESKKSN
                     LAILKINEEQLNKKSRIFDSAQSAETLKSSKDTTFASPETKNKQLPKSGESQNKVILW
                     SGIILLSIATMLSAKRFKQNRSL"
     gene            complement(505..1584)
                     /locus_tag="IXG44_001434"
     CDS             complement(505..1584)
                     /locus_tag="IXG44_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ1658160.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVIDFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVIALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(1693..2133)
                     /locus_tag="IXG44_001435"
     CDS             complement(1693..2133)
                     /locus_tag="IXG44_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ1658161.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTSLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTPQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(2190..2600)
                     /locus_tag="IXG44_001436"
     CDS             complement(2190..2600)
                     /locus_tag="IXG44_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAQ1658162.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(2781..3215)
                     /locus_tag="IXG44_001437"
     CDS             complement(2781..3215)
                     /locus_tag="IXG44_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAQ1658163.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVSFEERKQLFEKYM"
     gene            complement(3315..4163)
                     /locus_tag="IXG44_001438"
     CDS             complement(3315..4163)
                     /locus_tag="IXG44_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ1658164.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAVLGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLFFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFVFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            4552..6318
                     /locus_tag="IXG44_001439"
     CDS             4552..6318
                     /locus_tag="IXG44_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HAQ1658165.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETRRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVRDHLDYLTDLGVNGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVYDREDKLYFTRELEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            6420..7679
                     /locus_tag="IXG44_001440"
     CDS             6420..7679
                     /locus_tag="IXG44_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ1658166.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            7826..9127
                     /locus_tag="IXG44_001441"
     CDS             7826..9127
                     /locus_tag="IXG44_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1658167.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            9130..9981
                     /locus_tag="IXG44_001442"
     CDS             9130..9981
                     /locus_tag="IXG44_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1658168.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            9994..10809
                     /locus_tag="IXG44_001443"
     CDS             9994..10809
                     /locus_tag="IXG44_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HAQ1658169.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            10960..12126
                     /locus_tag="IXG44_001444"
     CDS             10960..12126
                     /locus_tag="IXG44_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ1658170.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            12356..13003
                     /locus_tag="IXG44_001445"
     CDS             12356..13003
                     /locus_tag="IXG44_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HAQ1658171.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            13087..14031
                     /locus_tag="IXG44_001446"
     CDS             13087..14031
                     /locus_tag="IXG44_001446"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HAQ1658172.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            14166..15617
                     /locus_tag="IXG44_001447"
     CDS             14166..15617
                     /locus_tag="IXG44_001447"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HAQ1658173.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            15638..16474
                     /locus_tag="IXG44_001448"
     CDS             15638..16474
                     /locus_tag="IXG44_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAQ1658174.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            16474..>17108
                     /locus_tag="IXG44_001449"
     CDS             16474..>17108
                     /locus_tag="IXG44_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ1658175.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLE"
BASE COUNT         5708 a         3029 c         3330 g         5041 t
ORIGIN      
        1 agaggtagat ccagaagaaa aaccagatgt gactccagaa ccagatacgg acgcaagaga
       61 ccaaggaata ccagaaaaaa taaacaaaaa aacaattcaa gaggatggaa aaaaagagag
      121 taaaaaatca aatctagcaa ttctaaaaat aaatgaagag cagctgaata aaaaatcacg
      181 aatatttgat agcgcccaat cagctgaaac cttaaaaagt agcaaggata caacctttgc
      241 tagtcctgaa acgaaaaata aacagctacc aaaatccgga gagtcacaaa acaaagttat
      301 actgtggagc ggaatcattc tattaagtat agcaacaatg ctttctgcaa aaagatttaa
      361 acaaaacaga tctctctaac atttgaataa tgtctgtgaa cgcatttttc aaataaaaag
      421 aaatatccag aaaacgaccc caaaatacca gattgaaatc tgatatttgg ggtcgttcat
      481 actttatctt tcttcatatt tattttaagg gatatctgtt tcttttggaa gatggttcgg
      541 gatctttttc actttcaata catctaaaaa gaaggtaaag atcggaatgc cgacaatcaa
      601 gccccaaacg ccaaagaacc gttcgctaac aagtaagacc acaaatgtat aaaaaatcgg
      661 cagttctgtt ttgctggaca tcaacttcgg attcagcaca taagactcaa ttagatggac
      721 aatcaaaatc gtaatcagga tataaacaac atctcggatg cctccttggg aataagctat
      781 gaaagataga ggaatacaag aaatgataac acctgctact gggatcagac ttaaaatgaa
      841 aatcatgatc gctaaactca gcagctgatg aaagccaaat acagccaagg cgattgtagt
      901 gagcaacgtg tttaaaagag cgataacaaa ttgagcttct aataccaaac caaatgtatt
      961 gacaaactta tcggcaaaat agtaaatatc ttgaaagaac caagaaaatt cactttttaa
     1021 aaacaatctt gagaatacaa ttgttttttt cttttcaatc ataaaaaaga aacttaagat
     1081 gaatgacata gcaaacgcca ctgccatttt tcctatatcc tgtaaatagc caagcgcaat
     1141 cgaagcgcca ttttgaagct gttcaaaaaa atcgtttcgc tcaatataag tatggatata
     1201 ttgtaaaaca ggatctgcat cttttggctg atgctcgtaa aaatcaataa cggactggat
     1261 catatcaaat gtttgatgaa taagcatcgg gatatacttt gtgaatgcca gatagataag
     1321 gtaaacgatc agtgtataaa tcatgatagc taaaacacct gtagggattc ggacaaatcg
     1381 ctgaatgatt tgaacaagat gaatcatcaa ataagtaaag ataaatgtga gcaaaatggt
     1441 tgtaatcata cttctaacta agaaaagaac aaaaacgaca aataataaaa cagtgagacg
     1501 gcgtattttt tcattcgcca aaaaacgctg ataaacggtc aaatcgacaa cctccaaata
     1561 acaaaaaata aacacgttat tcattatatg atggaatatg aaaataagaa agagaaagag
     1621 caaaaagaaa aaccgctaaa tgacgttatc ttatgagttg gttttcctca taaaatcgtt
     1681 catctagcag tttcagccct ttttctggtc ttgatgaaaa ttatctagca tttttcgggt
     1741 cagatgttgc agtgtatcca attcttcctg ttcaagtgga agataagact tgatcttttg
     1801 tggaatacct tctgcttgtt tttgcaacgc agcaccttga ggagtcagcg taacatttac
     1861 gattctttca tcacccgttt cacgctgacg attaatataa tttttttctg ctaacttttt
     1921 tagtaaggga gtcagcgtac cagaatccag acacaaatca tttcctagct ctttcactga
     1981 agtctttttc ttttcccata aaactaacat cacaagatac tgggtatagg taagattcag
     2041 cggcttgagg agggaagtgt agtgtctaat gatttcctta gaggcggcat ataaaggaaa
     2101 acatacttga ttaactaaat atagatcttc catttttcca cctccgctgt tttactatag
     2161 tgtatcatag cggatttaca caaaaaagtc tattcactaa ctgctttcag agtaacttct
     2221 atatttccag ctgtagcttt tgaatacgga cacactttat gcgctgcctc taataattct
     2281 tgcgtttctt ccagagaaag accttcgata gatccttcaa tctctacacc taagacaaag
     2341 ctcgtttcac tttctgaata aagggaaact tgtgcactga cagtagaagc cccagagata
     2401 tttttttgct gtttgatcaa ttctaatgca ctgttaaaac atgcactgaa accagctgca
     2461 aacaattgtt cagggttggt tgcattttcg acgcgtttac caggagctgc aatattcaat
     2521 tcgaatgtac gatcgggatt atgaacttct ccagagcgtc cgcctgtgtt gatcattttc
     2581 gtagaataca tttttttcat aaaattacca cctttaatta tattttatac aattgaattt
     2641 tacacaatat aattgtggaa agcaaagaaa tggattggca taagataaat aagagaaagc
     2701 acatgaaaca gacgcgcttt ctcgaatagc aaacatatac aaaaaaatat cagagacaaa
     2761 aactaatctc atcataaaca ttacatatat ttctcaaaaa gttgttttct ttcttcaaaa
     2821 cttacttcac gatggacctg atgaagcatc cataaataac caaaaggatc tctgaacatt
     2881 gagttagtca caccaaagtc catcatttct gtcatttctt gaatctctac acagccattt
     2941 cgtaatgctt tattatgtgt gtcctcgata tcagagacga tgatattgaa ccagatcgta
     3001 tttggatgtt cagcagtagg agcagtcaaa ccaagagacg tgttttcatc cagcatatgg
     3061 aatctcgtgc catacaaggt aaaaatgact tcattattgc ctctagggta atctgttgcc
     3121 tcaatcagtt gtatttcatc aaagattgtt tgatacagtt ctagtgcttt caaactatca
     3181 tttaatacaa aatctatttc cgttccttta tgcataaaga tctcctctcc ttttcagtcg
     3241 aacagttact agattctttt attataccag ttaaagaaaa cgcattcaaa aaaatagcgg
     3301 atttacttac caactcattc gattggatga taagtaaatc cgcgtcccat tacttctgct
     3361 acgctgctga caatgacaaa ggcacttgga tcgatttctt ctatcaatgg tttcattcgg
     3421 gaaaattcat gatcagtcac aacgatcaat aaaacctctt ttgactttcg attatacccg
     3481 ccttttgcct ctagcagggt aaggccacgt ccaatctcag cacttaagcg aaggcggatg
     3541 tcttccaaat aatgttcact catgaccata acagatttct ttcgattgat acctgtttcg
     3601 atataagtca tgacaattga agtgattgca atagataaaa tagcataaag aaactcttct
     3661 attccaaaaa caaacagatt aagagaaaca acgaatgcgt ctgtaaaaaa taagccaatc
     3721 gaagtattca agtggaaata ttttttaaaa atgaggggag ggatcgtcgt accgccgcta
     3781 gaagagttgt ttttatataa aatcgcaact cctaaagcaa aaatcgcact tccaaaaatg
     3841 acagacaaca accgatctga agtaatcatt gtttctggaa ctattgcaat gattaaagga
     3901 aaaaacaagc ttccgaaaag tactttaaaa aagggttttt tacctaaaaa tagaagagct
     3961 agtaccaaca taacgatatt caacactaaa ataactatag ctcggtcaaa tcctaaaaca
     4021 gcttccagta atattcctag accgctgaca cctccagcag caatatgatg aggccctaaa
     4081 aacatattga tagcgactcc taatatgaag agggcaaaaa taatcttaat gtatgtccat
     4141 ataaattgtc tcgtttcttt catttatcca cttccttaat tcattaataa tactcttatc
     4201 ttattataac ttacttttca ccttaagtta actttaatta ataaaaaaat taggctagtc
     4261 taaattatta tgtttcattc ttctagaata tacttctacg ttaaaaactg ttaaaaaact
     4321 tttactttag aaaattggag aaattgatcg cttaagataa attcaggaca ctaaatagta
     4381 caggaagtag attaaaactt ttaaaagttt taagtctatc tatttggcaa gtttaaaaaa
     4441 taaaaattcg gtatcaaaaa actgttaccg ataacatttt tgaaagcgtt gtcttttgtt
     4501 tctctatcac ttatagtgat gacgtcatat taaaacacaa ggaggaaatc aatggataca
     4561 gctgcaattt atcatcgacc ggaaagtgaa tatgcatttc tatatacgaa tacaagattt
     4621 cgtattcggc ttcgtactag gaaaaatgat atcaaaaaag tatatgtgct ttgtggagat
     4681 ccatatacca taacaactga aaaatggtat caaaaacaac gaccaatgaa aaaatgtcta
     4741 agtaccaatg ttcatgatta ttgggaaatt gaagtgacga gcgaaacaag gcgcttacag
     4801 tatgcgttcc atgttgttgg ggaagataac acggactgtt tttacggtga tcaaggaatt
     4861 tttccttatc gagaaaatgt gattactgaa ccaaattttt attttcgaat accttatttt
     4921 catcaaatcg atcgattcac tattccagaa tgggtaaaag aaacagtatg gtatcaaatt
     4981 tttcccgaaa gatttgcaaa cggggacaaa gcaaatgacc ccgaacacac tttaccttgg
     5041 ggaagtaaag atccagaccg agaagacttc ttcgggggag atttgcaagg tgtgagggat
     5101 catttggatt acttgacgga ccttggcgtc aatggcatct atttttgtcc catatttaaa
     5161 gccacatcca atcataaata cgatacgatt gattattatg aggtggatcc cgcttttggc
     5221 gataaaaaac ttttaaaaaa cttgattgat gaagcacata aaagagggat acgtatcatg
     5281 ttcgatgctg tattcaatca tatgggtgtc cattcaccac agtggcaaga cgttctcaaa
     5341 aacggagaaa aatcgatcta taaagactgg ttccacatcc gttttttccc agtagattct
     5401 tatcagatga ctgacttacc agaaactgct gaaaatatcc catatgatac ttttgctttt
     5461 actccattca tgccgaaact aaatacagca aatccagaag tgcaaaaata tttgctggat
     5521 atcgcaactt attggataaa agaattcgat attgatggat ggcgtcttga tgttgctaat
     5581 gaagtcgatc atcatttttg gaaaaaattc agagaagcag ttacagaaat caaaccagac
     5641 atctatatcc ttggagagat ttggcattcc tctcaagctt ggctgcaagg ggatgaattc
     5701 catgcagtga tgaattatgc attcacagat tcgattaaag attattttgc aaaaaagaaa
     5761 atcacagctt cacaaatggt cagtggaatg aatcatcagc aaatgcttta cagagatcag
     5821 gtgaacgaag gaacattcaa cttattagat tctcatgata ctgccaggat cttgaccctt
     5881 tgccaaggta acaaagaatt gatgaagtct gtgatggcct ttatgtttct tcaaaaaggg
     5941 tctccttgta tctattacgg gacagaaata gggatgactg gtgaagatga cccggattgc
     6001 cgcaaatgta tgatatggga gaaggaggaa caagatctag agctattcgg ttttataaaa
     6061 gaacttgttt ctctaaggaa acagttgtct aagattattt ctgaaggttc tactcagtgg
     6121 ctaatagtat atgatcgtga agataagctc tatttcacac gggaactaga gggacagatc
     6181 atctatgttt actttaatca gtcaaaagaa ccttgggtag tagaacagga aaatgaggtg
     6241 attcttagtc aaaattgtca gttgttagaa gacgggaaag cagagatacg acaatacgga
     6301 tttttgatca tgtgttgaaa actgttaccg gtaacaacaa gaaagcgttt gcaaatgaag
     6361 atttgtgccc attataataa gggtgtaaca gcacgttgaa tacacaggag gaaatgaaga
     6421 tgaaaaaaag aagtgtgaaa cagttgggtt tagggttaat ctctttagga atcttaggcg
     6481 gattagcggc ttgtggcaat ggtaactctt cagcagaagg ggaagccgat gaaggaaaaa
     6541 cgttaacagt ttccgttgat tctggatata aagattatat aaacgaaata aaaaacactt
     6601 tcgaaaaaga aaacgatgtg aaaatcaagc tagtagaaaa ggacatgttc gatcagttgg
     6661 aatcattagc gttagatggg cctgctggaa agggacctga cgttatgatg gcagcatatg
     6721 accggatcgg tgcgttagga cagcaaggac atctggcgga agtaaaactg ggaaatgaag
     6781 cagcttacga tgaaacagac aaagcccaag tgacttatga tggaaaaatc tacggagaac
     6841 ctgcagtaat cgagaccctt gttttgtact ataacaaaga tttagtcgat acagcaccat
     6901 caacgtttaa agatttggag aatctatcca aagacagtcg ttttgctttt gagtcagaag
     6961 caggaaaaaa tacaggattt ttagctaaat ggactgactt ttattattcc tatggtttaa
     7021 ttgcaggtta cggtggctat gtatttggtg atgatggcac aaatccttca gatatcggtt
     7081 tgaataatgc aggtgctgta gaaggaatct cttatgcaac agattggttc aaaaatgttt
     7141 ggccaaaggg aatgcaagac ataaaaagtg caggagactt cgccagccag caattcatgt
     7201 ccaataaaac tgcagcaatc attgacggtc catggcaagc gcaaacatac aaagaaaaca
     7261 atatcaatta tggtgtagcc aaaattccta ctctaaataa tggacagcct tatcaaccgt
     7321 ttggcggagg aaaaggatgg gtagtcagca attatgctaa aaacaaagac ctctctcaaa
     7381 aatggcttga ttatgtaacg aatcaggaaa atcaagaaaa attctttgaa atggtaaatg
     7441 aaatccctgc aaaccaacaa gctagggaaa cagcaaaggc taaaaacgat gaattaacaa
     7501 cagctgtgat tgaacaatat gaaacagctc aagcgatgcc aaacatccca gaaatgggcg
     7561 aagtttggac aggtgcagaa aacttgatgt tcgatgcagc atcaggttca aaaacaccaa
     7621 aagaatccgc agatgaagca gtaaaaacaa tcagtgaagc catagaacaa aaatactgat
     7681 taaaatgata accgtccatt tttataaaaa atggaacagg tgcttcagat aaaagtggca
     7741 agagcgagat ggaaaattat cttgctcttg ttgccattat cttaaaagca gtttgcggtt
     7801 tagcaagttt agagggggca ttcagatgca gcaagataaa acaagcaata tcaacgaaaa
     7861 aaaaatcacg ctttgttcac ttattccagg gatggggcag tttgtaaatg gccaagcttt
     7921 taaaggtatc gtttttttcg ccatattcgt cctattcatt ggtcagatgc ttctgggcgg
     7981 atttcatgca ttggtaggat tggttacttt gggaagtata ccaatggaag atcattcatt
     8041 atttattttg attgaaggca ctttgcaatt catcattaca atcattttct tgatttttta
     8101 cggtttgaat cttttagacg cttatcgagt agcgaagatg cgtcaagagg gcaaaaaaat
     8161 caatcggacg atgaaggaag tcatacataa tataggtgat gaaggttttc cttatctatt
     8221 gacacttccc gcttatcttc tcatgatttt tacgatcatt ttcccagtcc tagttacatt
     8281 gttcactgca tttactaatt atgacttcaa tcatattcca ccagccagtt taattgattg
     8341 ggtaggggct aaaaactttt ttagtatctt ctttttaagc tcatatcgta atactttttt
     8401 tgcagtcttt tcgtggaccg tgatttggac tttatgtgca acaactttgc aaatcaccct
     8461 tggtattctc acagcggttg ttgctcacca gaaatttatt aaattcaaac gtattttcgg
     8521 tatcattttc ttattaccat gggcggtccc agcttttatt actatcatga gtttttcgaa
     8581 tatattcaac gatagcgtag gtgccatcaa tactcaagtg attcctttca taaaccagct
     8641 tccttttgta gatatcagtg caatctcttg gaaaacggac ccgaactgga cgaaagtagc
     8701 aattatctta gtgcagggct ggttaggatt tccctatatc tatgtgatgg ttacaggaat
     8761 cttacaatcg atttcagaag acttatatga agcagcaaaa atcgacggag caaatgcatg
     8821 gcagcgcttt tcaaacatca cgcttcctgc tgtcttccta gtggcagcgc caacatttat
     8881 tacacagtat acggggaatt tcaataattt ctccatgatc tatctgttta acaatggtgg
     8941 tcccggaagc gtaggcggag gagctggttc tactgatatt cttatttcct ggatctataa
     9001 acttacgact ggaacttctc cgcagtattc tatggcttca gcaatcacgt tgattatctc
     9061 cgtcatcgtt atctgtgtgt cattgctggt atttaaaaag acaaatgcgt tcaatatggg
     9121 ggattaacaa tgaaaaaatt tcattcgagt gctcgattcc aaaaacgact ttctcacttt
     9181 tttacttact tttttctttt aatactgtca gtcattatca tctatccgct tttgatcaca
     9241 gcaagtactg ccttcaagtc aggaaatatt gcagctttta gtttagatct ttcaagtgac
     9301 tggactttag gaaactttac tcggctattc agtgaaacac tttacgggac ttggtataaa
     9361 aatacattga tcatttctgt cctaacaatg atcgtacaag tgactgtagt aactttggcg
     9421 ggctatactt atagtcgcta tcgttttatc ggccgcaaat caagtttgat gttcttttta
     9481 gtgattcaga tggtaccgac tatggcagca ttgactgcct tttatgtcat ggctttctta
     9541 ttagaagcgc tcgataagta ctggttcttg acaatgatct atataggcgg tggaattcca
     9601 atgaatactt ggctgatgaa aggatacttt gacactgtac caatagattt agatgaatct
     9661 gctaaacttg atggggcagg gcatttcagg atttttttcc agattgtatt accgcttgtc
     9721 aaaccgatgc tcgccgttca agcattatgg gcttttatgg gaccttttgg ggatttcatg
     9781 ttagctaagt tcctgcttcg aacacctgaa aatcagactg ttgctgtcgg cttacaaaca
     9841 tttatttctg atgtgacaaa ccaaagagtc tctctttttg ctgctggggc aattttgatt
     9901 gcactaccga tttgtctatt attcttcttt ttacaaaaga actttgttac cggcctgaca
     9961 tctggcggaa caaaaggata ggagcagagt atcatgcaaa catttccgtt aaactatttt
    10021 tccgcattac gtacgccaac tcaagtgttt gcgggacgca aactattatc atggcctaaa
    10081 tttttcttga tatttgtatt tttagtttcc ttgatggtca tgcctgtgac tcttttttat
    10141 gccaatcaga tccaagcgat tccattagaa caatttctat ccgttcattc gctgatcgat
    10201 gaacaaggaa cccaaaaatt ttctgaactt gaattatctg aaactgggtt acaagcatcg
    10261 caacaaacga tagctgtgac ccctgaaatt ttggtaggtg tgtctttatc tgagaaacaa
    10321 caatccgatc atggaacttt tattgatttt gagaaggaac aatgggtgat ccaacaaaag
    10381 gataaatctg gtatacgccg ctatacgatg aattatagcc catctttcca accagattct
    10441 gtacgtacgc ccgaagattt ccaacgattt ttagaaagag aattttatgc cagtaatcgt
    10501 ccaacaatca ttttatctta tagtttaagt cttggtcttg tcctatttgt catgacgagt
    10561 ctcattttat tcggggcttc ctttttcttg tggatgacaa gaaaaagcca actttcatcc
    10621 attcatacat tcaaagagag tgcaaatttg atgctgaatg ttatgggaat aggttccatg
    10681 gcagcagcag ttgtagggtt tatccacttt gactttatct tgatgctaag tgtccaaaca
    10741 acgataactg tcttgctgct tttatgggtt tttgtcaaaa caagattcaa agataagcgt
    10801 gtcgaatgac aagtttaagc gatttatgcg acaatgaatg tgaaaaaaat gaaaaataaa
    10861 gtagtttttg aaaaatttct gaaaaaatta aacttcttag ttgattcttt tcttttttag
    10921 tattaacgta atagggtagt ttttttaagg aggaaaatca tgttagagaa aactttatac
    10981 aatcaattat tttcacgttc tttctcgctc cctgtcgaag taaattattg ggacggtaca
    11041 acgaaacaat acggagatac ggacactcct ccccaaatca aaatcaatat ccatgaagaa
    11101 atcccaatga aagagattac aagtaacgct tctttggcac taggagaagc ctatatggat
    11161 catcgaatcg agatcgaggg aagtatccaa gcattgatca acgatgctta tcagcaagca
    11221 aacagctttt tacgaggaaa agattatcta aaatggctgc caaagaaaga aaaacatacg
    11281 aaacaaaaaa acaaagaaga tatccatgcg cattatgact taggtaacga cttttataaa
    11341 ctttggctag atccaacttt gacttattct tgtgcttatt tcaatacacc tgaagatacg
    11401 ttggaacaag cacaaatcaa taaagtccat catatcttag acaaactctt tatcaaagaa
    11461 ggcgaaacgt tgcttgatat cggttgtggc tggggaacat tgatgttcac tgctaccaaa
    11521 gagtataatg tcaaggcaac tgggatcaca ctaagtgaag aacaatacga tttgattacc
    11581 aaaagaataa aagaagaaca tttagaagat aaatgccgtg tattattaat ggattaccgc
    11641 gaactaaaag gcgaaacgtt tgatcatatc acaagtgtag gtatgttcga acatgtaggt
    11701 tctgaaaatc ttgaaggtta tttcaaagtg gtaaaagatt tattgaaacc aaaaggaaca
    11761 gctttgatcc atggaatcag ccgacaacaa ggcggtgcga caaatgcttg gatcaataaa
    11821 tatattttcc ctggcggtta tgtccctggt gttactgaat tagttgataa tattacaaaa
    11881 aacgatttgc aactgattga tttggaaagc ttacgtcgtg actaccaatt gacattagaa
    11941 cattggacaa agaattttca tcaagtccaa gatcaagtca cgaaagaaat gggtgattcc
    12001 ttctatcgaa tgtgggatct atatttacaa gcttgtgcag cttcatttga atcaagtaat
    12061 atcgatgtga ttcaatattt gcttgttcaa cctggaaaca atgacatccc aatgcatcgt
    12121 gcataattga ggcaaaagta ctgtttaaag actatttaaa aagaagctaa gttttatgat
    12181 gctccagaag tgaacttttt tgtctaactt ctaggattgt ctctaaagct tagtttcttt
    12241 ttctttatat tttcataata aaagtttggt tctttaattt ttaaacatga cagaaaattt
    12301 tttcttcgtt atacttgtaa tagaatacta ttttataaca aaggggatcg aatcaatgaa
    12361 aaaaactgtt atttatacta gtttatttgc agctttgttt ttagctgctt gtcaatcaaa
    12421 taccaatcaa ggcacatctg ggaatacgtc ttccagtagc gaacagcgct cttcacaggt
    12481 atcggactcc tcaacaaaag caagcacatc ttcgtctagc cagacaaaaa gttctagtga
    12541 agcaagtact cagtcagata tcaaagtgtc tgcagctcgt gcgatcgaat tgttccaaga
    12601 taaataccca gatactgcaa ttactagttt ggaattagat agtgattggg gatcttactt
    12661 ctacaagata gaaggtgtcg atgatcaaaa agaatacgaa gcaagaattg atgctcaaaa
    12721 tgaaaaaatg gagacagaga atccagaaac tctagatcga gatgaacaaa atggtcaaaa
    12781 gaaaaaagaa gatggtctgg atgttgataa attgatcacg attgaggaag cagcaaaaat
    12841 cgccgtcgac aaagtaggaa gcggtacggc aacagattgg gatttggata aagatttagg
    12901 tactacttat tgggaagtca aagtaaaaga cggcaatcaa agtacatcag tcaagatcaa
    12961 tagccaaaca ggcgaagtac tgtcaactga acaagatgat taagaatttc ttcggaaaac
    13021 aagagttgat ttttctttta gtcatgatac actagacaaa ataacttgtt tgaaggagcg
    13081 atttacttga gtttagaaac gacctttgcc aatcatatct ttgccaatcc tttgatgaat
    13141 gcatctggtg tccactgcat gactacccag gaattagatg aactggctca ttcagaagca
    13201 ggagcattta tcactaagag ctgtacgatc aatgaacgaa aaggaaatcc cgaaccacgc
    13261 tattttgatg ttcctttagg gagtatcaat tctatgggac ttccaaactt aggtttttct
    13321 tattatctag agtatgctct tgcgtatgaa aaagtgcagg aaaaccaaaa tcaaccgtta
    13381 tttttttcca ttgccgggat gagtgtccag gaaaacttgg aaatgcttga gaaaatagaa
    13441 aaaagtgggt ttaatgggat cactgaatta aatttatctt gtcccaatgt gcctggaaaa
    13501 ccacaactag cttacgattt tgaggccact tatgaaacac tgaaagaagt attcagcatc
    13561 ttttctaagc cgctaggaat caaattgcct ccttattttg attttgctca ttttgatcag
    13621 atggctgata tcttgaatca gtttccattg acctatgtca atgctatcaa cagtgtgggc
    13681 aatggtttgt atatcgatac agaacaagaa gcagtagtca tcaaaccaaa agaagggttc
    13741 ggcggcattg gcggagagta tatcaagccg actgctttgg cgaatgttcg cgctttttat
    13801 acacgtttga aaccagaaat ccaaatcatt ggtacaggtg ggattcggac aggacaagat
    13861 gcatttgaac atctgctatg tggcgcaagc atgttacaga ttggtacaga gcttcataaa
    13921 gaagggccgg aaattttttc tagaatcata aaagaattaa cgcagattat gtcagaaaaa
    13981 ggatatacgt caattgacga atttaaaggc aaattacgca ccatttcata gcaaagagac
    14041 gaaggtcatg attgatcttc gtcttttttt ataaaaaaca ctcgtctgtt cattcctgtg
    14101 caagcgtact cattgtatgt tagacgaact tgtgggaaaa taacagaggc aaaaaggagg
    14161 ataatatgaa tgatgcaaca gaattagcaa aagagcttaa agaaaaagaa tttagttttc
    14221 aagaactagt tacggatata cagaaaaaaa taaagaagca aaatcatgaa ttgaatgcgt
    14281 ttgtcgcgct tgaaccattc gagagtaatt cccacgagct attgattccc cagtcagaaa
    14341 aagggccatt ggctggtatc ccatttcctt taaaaatgct cggtcaagaa aaaaagggat
    14401 ggctagctac ttcaggatct cgtttatttg aaacacaccg agcttctcat acatctaatt
    14461 atgtgcggca agcagaagcg ataggtctag ttccttttgg acaaaccaat gcgccagagt
    14521 ttggtttcaa aaatattaca gacccagtca tctatggccc agcaagaaac ccttggaacc
    14581 ttgaatgtac accgggcgga tcaagtggag gagctgctgc tgcagtttca tcaggaatcg
    14641 ttcctttagc tggtgctagt gatggcggtg gttctattag aatacccgct tcgttttgcg
    14701 gattgattgg gctgaaacct tcgcgaggga cgatgccagt tggtccatat gcttggagag
    14761 gatggcaagg agctgctatt gatttcggct tgacagtttc tatgagagac acggaggctt
    14821 tattttatgg gatgagaagt atccattccg gtgcgccata tcaagtatcg ccagttgagt
    14881 ggcaaacaca tccaagaaaa aaacggttga agatagcttt ttgtgttgat tcaccgatcc
    14941 actcacagat tagtgaagag gcagtacttg ctgtaaaaaa agctgtagat ttccttggaa
    15001 aagaagggca tgatttgaca gagattcctt acccactaga tggtcgtaaa ctgatcacgt
    15061 cttattatca gatgaatggc gcagaaacag cagcaatgat ccgctcgatt gaaacaggca
    15121 tccagcgagc tgtcagaaaa gaagaacttg aacccatttc ttggactttg ttccaatatg
    15181 gtcagaagct ctctgccgct acttacatcg attctctgca tgtatgggat gaagcagcaa
    15241 ttgtcatgga aaacctacat caagaatacg atctattcct ttcaccaacg actgcattta
    15301 ccgcccctaa aatcagcgaa gatttgcaaa gtgatgcgat aagagcacag atggaacaca
    15361 tcagtgattg ttctgaagta gaaggtttag acgttgtttc ttccatgttt gaggagagct
    15421 tgaagatcac accttataca cagttggcta atctgactgg acaaccagca atcagcttgc
    15481 ctactcattt gacaaaagaa aacatgcctc taggaatcca atttatggct gccagaggaa
    15541 gagaagattt gctatttcaa atcggctatc tatttgaagc aaatcatcag ttccatttac
    15601 caaatccatg gaaatagcag aaacaaggag aagaagaatg aagaaaaaaa gattgtttgc
    15661 tctaataact agtgcctttt ttattttaag cgcttgcggt acagctgccg ctaatcagga
    15721 agataacagc tggagtaatg tagaaaaaaa gaaagaatta gtagtagcga cttctggcac
    15781 actgtttccc gcatcttatt ataatgatca aaatgaatta gttggttatg atgtagattt
    15841 ggcaaaagag gtggctaaac ggttaaatgt caaaatctcc tttaaagaat acaacgtgga
    15901 tggacaaatc acttctgttt caaaaagaga atcagatttt gctgcgaacg attttggtat
    15961 cacaaaagaa agagaaaaaa aattctccct ttcagagact ttcaaatatt catttgacag
    16021 tatgatcgta cgaaaaaaag atgattcagg aatccactca cttgaagatc ttaaagggaa
    16081 aaaagcagct ggcgaaccca atactagcta tatgcgttta gcggaaaaac acggagctaa
    16141 attagtcaca tatgacaatg ccacgaatga tcagtattta agcgatgtag caaatggtcg
    16201 aacagatgtt attctaaatg attattatct gcagaaaatg gccgtatctg ctttaccaga
    16261 tatccctgta aaaatactgg agaacgttta tttcaatgaa aacgaaagtg gttttttatt
    16321 gaaagaagga aatgatgctc tgaaaaagaa aatcgatggc gtattaaaag agatgaaaga
    16381 agatgggacg acaaagaaaa tatctgaaaa atattttcaa actgatgttt cagtcaaacc
    16441 gaaagaaacg atggctgagt cggtgagtga gtgatgtact ttcctctaat tgatttttcc
    16501 ctcatgtgga cttcacttcc tttcgtactg caaggcttgg tatacacatt agggattggc
    16561 ttcgtgtcgt ttcttttagg aaatttggta gggttgcttt tgacagtttt aggtcttttg
    16621 gattggctgc ctctgaatgt atttattcgt ttttatttat cgttctttag aggcattccc
    16681 gcactagtcc tgctgtttct gttatatttc gggttacctt atcaattaag tgccttaacg
    16741 gcttcagtca tctgctttac gattaccagc agtgcattta ttggtgaaat ctatcgagga
    16801 tcactagcag gcgtttcttc aggtcagtgg gacgctggtt tagcttctgg tttttctttc
    16861 tttggggtat tgaaatatat cgttttgccg caagcttttc gaatctcagt accggcactc
    16921 agtaacgtag ctatggatct agtgaaaggt acttcgttag cagcaacaat cacagtgcca
    16981 gaaatatttc aaaaagcaaa gatcataggc ggtcggacat tcgattattt ttctctctat
    17041 attttagttg cactgattta ttgggtcatt tgtatcggga tcgaaaaagt gcaacagtat
    17101 ttagaaaa
//
LOCUS       DACEUZ010000052        17006 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.052, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000052 DACEUZ010000000
VERSION     DACEUZ010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17006
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.052"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(<1..124)
                     /locus_tag="IXG44_001450"
     CDS             complement(<1..124)
                     /locus_tag="IXG44_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HAQ1658176.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVF"
     gene            complement(228..539)
                     /locus_tag="IXG44_001451"
     CDS             complement(228..539)
                     /locus_tag="IXG44_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAQ1658177.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPSPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDKSQ
                     F"
     gene            complement(541..738)
                     /locus_tag="IXG44_001452"
     CDS             complement(541..738)
                     /locus_tag="IXG44_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterocin"
                     /protein_id="HAQ1658178.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(1132..2859)
                     /locus_tag="IXG44_001453"
     CDS             complement(1132..2859)
                     /locus_tag="IXG44_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ1658179.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2852..4045)
                     /locus_tag="IXG44_001454"
     CDS             complement(2852..4045)
                     /locus_tag="IXG44_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ1658180.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(4233..4877)
                     /locus_tag="IXG44_001455"
     CDS             complement(4233..4877)
                     /locus_tag="IXG44_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1658181.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4971..5105)
                     /locus_tag="IXG44_001456"
     CDS             complement(4971..5105)
                     /locus_tag="IXG44_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HAQ1658182.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(5107..7239)
                     /gene="feoB"
                     /locus_tag="IXG44_001457"
     CDS             complement(5107..7239)
                     /gene="feoB"
                     /locus_tag="IXG44_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HAQ1658183.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(7236..7709)
                     /locus_tag="IXG44_001458"
     CDS             complement(7236..7709)
                     /locus_tag="IXG44_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HAQ1658184.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            8110..8334
                     /gene="nrdH"
                     /locus_tag="IXG44_001459"
     CDS             8110..8334
                     /gene="nrdH"
                     /locus_tag="IXG44_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HAQ1658185.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            8493..10652
                     /gene="nrdE"
                     /locus_tag="IXG44_001460"
     CDS             8493..10652
                     /gene="nrdE"
                     /locus_tag="IXG44_001460"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HAQ1658186.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            10702..11667
                     /gene="nrdF"
                     /locus_tag="IXG44_001461"
     CDS             10702..11667
                     /gene="nrdF"
                     /locus_tag="IXG44_001461"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HAQ1658187.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(11756..12895)
                     /locus_tag="IXG44_001462"
     CDS             complement(11756..12895)
                     /locus_tag="IXG44_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ1658188.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(13204..13917)
                     /locus_tag="IXG44_001463"
     CDS             complement(13204..13917)
                     /locus_tag="IXG44_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HAQ1658189.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(14171..14872)
                     /gene="nagB"
                     /locus_tag="IXG44_001464"
     CDS             complement(14171..14872)
                     /gene="nagB"
                     /locus_tag="IXG44_001464"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HAQ1658190.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            15106..16638
                     /locus_tag="IXG44_001465"
     CDS             15106..16638
                     /locus_tag="IXG44_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ1658191.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16829..>17006)
                     /locus_tag="IXG44_001466"
     CDS             complement(16829..>17006)
                     /locus_tag="IXG44_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1658192.1"
                     /translation="VFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQ
                     TVVFKGKRNLENKK"
BASE COUNT         5279 a         3496 c         2908 g         5323 t
ORIGIN      
        1 aaaaaacaca ttccactaaa tttgatgccg gttttccttg aggttttgtt ccagcacctc
       61 ctttaatttt tttaagctct tgatctgaac attgtttagc atttagtcta tttttttctt
      121 ccattacgat tccccctaca aactataaat acttttatgt cactgattca atatacctat
      181 ttttctaatc aaaaaatttt gagacatatt taatattata agtatcatta aaattgagat
      241 ttatctccat aatctgctcg atataaacca tttattccag cagttcttcc aatttcactc
      301 aaatttctta ttaattcctc ttgatattca gtgaatctta ttttattagt ataagcagta
      361 aaatatagat aattcaccaa tctatttatt aaaggactgg gatctaattc atatttttgt
      421 ttttctaatt ttaaatatac cttttgtaaa acctcaagaa tatcagaata tttaggatct
      481 ttacttatag atatatcatt atataattca tgaacaattt gcttagcatt ttttttcatt
      541 ttagcacttc cctggaattg ctccacctaa aaaaccacct atagacattc ctgcaataca
      601 agtagttgcc ttggcccaat cgaccgtaca tttattttta gtgcaataca ctccatttcc
      661 ataatatttt ccactatgag tcgtaccccc atagataagt tgtgtctctt taatagacaa
      721 aatttttaaa tgtttcataa tctaatcacc cctaaattaa tgtttttaga ctattattct
      781 aacattcatt aaatttcaaa aaacaattcc ttcttgaaac gtagcaaaat aattcctgaa
      841 taagaattta atcactttta agtatataaa ctatgtatat tctttaatat tttttgagaa
      901 tgcacttcgt acataataaa ctttaatcac attagtcttt catacctacg atcgatctct
      961 ttgactcgca cccataaatt cataatagtt tgtcggcgtc tttgaacgtt tcgaagatga
     1021 gctacgcatt ctcttttttt atagacaaaa aagatagaga cgaacatgga gcttccaaat
     1081 ttggatattt gatccaattt tggaagctcc atttatttcg tgtctgtctt tttatttttt
     1141 ccagctttcg actaattctt ggttctcttt gatccagtct tttgctgctt gctgtggatc
     1201 ttttccattg ttgatttcca acataacttt ttccatatct ttttctgacc aatggaaatt
     1261 gtctaatact ttgtaagctt ctggctggtc ttctttcaat ccttttcgaa ccatcgtatg
     1321 gatctgctct tcgcttccca ttccgttttc aggatcttct aagtatttca gatcatattt
     1381 ggcaaacatc caatgaggtg tccatcctgt aactacaatt ggttcgtgtt ttttgatcgc
     1441 ttgacctagt gcaacggtca ttgctcctga agaagatgtc tctactttcc agtctttcaa
     1501 gttgtcatat ttttggatcg tgttttcagc agccgtaact acaccagctc ctggctcgat
     1561 tcctatgatt ttcttacctg cctgatcggt taactcatcg atcgaattta catccatgta
     1621 ggctggcact gctaaaccaa cttttgctcc tgtcaaattg gcacctaggt cttcgacttg
     1681 atctttatat tgtgcatatt gtgaaccatg agttttaggt agccaagctg aaaccatggc
     1741 atctgactcg ccatttgaaa cagatttcca catgattgaa ttatctaatg gcgtcatcgt
     1801 tacatcatag cccatttgtt tcaatacttc accgactaca tttgttgaag caacttctgt
     1861 atcccattca acataagata agttgatacg ttttgtttct ttggcagatg aaaaactccc
     1921 tataccaatc gcacctaaga tcactactac tacagcacca atgatcagtc cttgacgttt
     1981 tttacttttc tgtgttgttg ctcctgcagc ttttttattg tttggctggt tcaaatgctg
     2041 tgtaaaacgg tcaatgatga ttgctaagat aactaatgca accccgttga caaatccatt
     2101 cccgacttgt gctctttgca atgctgataa tacgccgcgt cctaagccgg gtgcaccgat
     2161 catagaggca ataacgacca ttgaaagtga cagcattgtt gtttgattga tccctgccat
     2221 gatcgtactt ttagctaaag gcaattctag tttgaatagt ttttgacgac ttgtgctccc
     2281 gaatgaatca gaagcttcta ctaattcttt aggaacttgt cgaatcccta aattcgtaaa
     2341 acgaacggtt ggtggcagag caaagatgac tgaggcaaat acccctggaa ccatcccgat
     2401 accgaaaaat gcaactgctg gaatcaaata aacgaatcct ggcattgtct gcataaagtc
     2461 aaggatcggt ttgataatac tttgggcttt actgcttttt gccatcaaga tccctaatgg
     2521 taccccaatg atgatcgaga tcacactaga taaaagaaca agtgtgacag tattcatcaa
     2581 atcattccat agaccttggt tataaataaa ccatagaccg attaatgtga acaacgtaag
     2641 tcctggacgt ttcttagaaa taaagaaagc tgctaccgtc aataacaaga taaacaaagg
     2701 agctggaatc actaacagta aatttgtaat ccccgacatc actgcctgtc caatcgtttg
     2761 taaaaatgaa aataggccag aaaaagttgt cgtaaaccat tctgtgatat tttctaccca
     2821 gtttgccact ggtaattgat agttcaatag attattcatg ttcactcacc tcgcttgttt
     2881 ctgctaatgc ttcaatcaca ctacctcgga tgacgacacc ttttaatttt ccatcctcta
     2941 caactgcaag tggagtcggt gagtcataaa taatattgaa aatatcactt accaccatgt
     3001 ctttcccaat caccgtcaca ttttcatcta ggaagtcgat caatggacgt ttttgcttcc
     3061 tagcttctaa cgcttgatct gcagtgatga tccctttcag gtgtctcttt ctgtcaacag
     3121 ccatcagcat gctgacttct tcattacgca tccttgtcaa tgcaacgttt ggtccatcag
     3181 attcgatatt cgtcgtcaat gcaggtacca tgatgttttg agccgtaagc actttggaac
     3241 gatcgacttc ttccacaaat tcacgtacat aatcatttgc tggattggtc aatatttctt
     3301 cacctgttcc gatctgcatg atttcgccgt ctttcatcaa ggcgattctg tccccaatcc
     3361 gtaaagcttc attcaaatca tgggtaatga aaataatcgt tttttgaaca ttggcttgca
     3421 ggtccagtaa ttcgtcttgc atttctcgcc ggatcagcgg atcaagagcc gagaacgctt
     3481 cgtccatcag caagatctca ggatcatttg ctaaagctct ggctaggcca acccgttgtt
     3541 gcatcccacc agataactga ctaggatatt ggtctttaaa tgaaagcaaa ctagaatttt
     3601 ctaatgcttt ttctgctttt tcttgtcgtt cttctttagg tacaccacgg acttctaacc
     3661 cgtattctgt attttccaag atcgttcgat gtggaaacag accgaagttt tggaaaacca
     3721 tattgatttt atgacgacgg acttctcgca attcttcttt gcttaacttc gatacatctt
     3781 ctccatcaat atagatactg cccgaagttg gctcaatcag acgattcaat agacggatca
     3841 atgtggattt cccactcccg gaaagcccca tgatcacgaa gatttctcct tcttttacgt
     3901 caaaattcac gtcatacaca ccaaccgttg caccggtttt ctttaaaatc tccgttttcg
     3961 gctggtgatc attcatcata tctaaagcgg cttgtgtctt tttaccaaat atctttgtta
     4021 aatgttttac ttctactttt accaaacaaa ttctcctttt ttatgtaaat tcatttttct
     4081 ttctgaaaaa aagtgaccac actatgttaa catcatgtag tcactttgtc aaaaatgaaa
     4141 gaaatttgct tgtccaaact gaaaaaaact tggctaaatc tttgtaaata aaaggtttag
     4201 ccaagttaat atttttttaa tatttcacac aatcattctg caagctgatg ctccgggtaa
     4261 aaaaagtttg tcatacgctg tagtgtatat tcatttccaa taaaatgaac gatgtcccct
     4321 gccgtaaatt tagcataggg gccaggtgaa acaagcagtt cttcattatg agaaattgcg
     4381 ataactgtgg cgccagtcgc ctgccagata ttcaactcgc taattgaacg gtccagatgc
     4441 attgcttttt ctgataaaga taattcaaac ggaagtaaag gattcatctt atgtattcgc
     4501 ttcgtttgat tgaccagctg atccaataat tgagaaaaat gattcagctc atcttgttgc
     4561 ttctccacac ttgccatgat ttctgctttt gtatcttgga tggattgtac atcttgatat
     4621 tgcaatagga aatcttttgc tttttccttc gaagcaacaa atgccccact tccatgccgt
     4681 acttccatga ttccaagatc taccagcaca ttgatagcct tgcgggtagt ctcaggagaa
     4741 gcattgtacg tactggcaag tgttgagcgg gcatggatct tctcacccac tttatatcgg
     4801 ttttcaacga tacgttcggc taaatcaaca gcaatctgct ggtagcgcgg gcggacgatt
     4861 tttttctttc cagacatttt ctttcctctt tcttttttta ctttccctca tcttactatg
     4921 ctctagcaaa ataagcaaaa aaggggagaa tagcctcttg caacgactga tcatcgcttg
     4981 atctgttcct tttttactgg gcaagctgtt tgacagtcat cacaattctt accggatttt
     5041 aaacgagtat agacaattgc gcctgcactt ccaaagatta acacacttaa taagaatgtc
     5101 gccatcctat tcctctcctt ctaatgacaa tatagtataa aactggttct tggattttgg
     5161 ctgacggaac aaaacataaa cgagtcctat catcagtaaa atagcaagat acgtccctaa
     5221 cgctacctgt ccaccctcca ataagtgtcc caactgataa atcacaaaac taactaaata
     5281 agctaaaccg cattgatagc ctatggcgat ccaagtccat ttcttggcat tcatttcccg
     5341 atggatagcc ccaattgccg caaaacaagg cgcacaaagt aaattgaagg ctaacaatga
     5401 ataagctgct gcgggagtat atgtgtgttg tagcacttgc cagatttctc tgccattttc
     5461 ggatacttcc gcatgaccaa acaaaatacc aaatgtgcca ataatatttt ctttagcgac
     5521 caatcctgta atcgctgcga ctgttccttg ccagtttccc caaccaagcg gctgaaaaac
     5581 aactgccagg attcgcccga aaaaggctaa gatactttgg tcttcagaaa caggttgagc
     5641 ataaaaatta taagaagagc tgaaccagat cacgatacta gaaacaaaga taatcgttcc
     5701 tgctttttta acaaaagact tgctgcggtc ataggtttgt cttaggaccg ctgaccattg
     5761 aggaaaatga tatgcgggca attccatgat aaaaggagct gtatctccag caaacaaacg
     5821 tgtttttttc aagctgattc cagagaaaat aattgcagtc ataccaagaa aataggcaga
     5881 aggagctacc caagattgat gaggaaaaaa ggcccctgct actaaggcaa taatcggcaa
     5941 ctttgcagaa caaggcatga aggttgtgac catgatcgtc aaacgacgat cttgctcgtt
     6001 ttcaatcgtg cgactggcca tgacgcctgg cactccacat cctgtagcaa tcagcatagg
     6061 aatgaaggat tttcctgaaa gaccgaattt cctgaaaagt cgatccatga caaaagcgat
     6121 cctcgccata tatccgcagt cttccagaaa gctcaagcat aaaaacaata taatcaattg
     6181 cggtagaaat cctaacactg ctcccacgcc tgcgatgatg ccgttcaaga tcagctcctg
     6241 catccatccc gctacttgcc attcagctaa caatctgcca acatgttctg gtaaccatgt
     6301 accaaacaat gtgtcattga tccaatctgt ccccattgtt cctactgttt gaatagccag
     6361 ataatagatc agccacatca caaatgcgaa gatcggcaag gctaaccaac gattcgttgc
     6421 aatctgatcg attttgtcac tcatcgataa ttggaaagaa tcatttgaca caacacagag
     6481 tgtacatagt tgggtaataa attcataacg ttcattgacc agaatcgtct cactatcgtc
     6541 gtccaatagt ttctctgtta actggatcag ttgctcgatc tctttttgct gagaaactgc
     6601 tatctcgtaa tcctctctta ccttctgatc tcgttcaaaa tatttcattg cataccagcg
     6661 tttcttgccg ctttgttctc ctagtacatc ttcaatttca tgtaaagctg cttctaaacg
     6721 tggatcatat gtacagggaa caggaataga aggccgcttt tttgcagact cctgaatctt
     6781 ttcgattagt ttttcgattc cttgcttttt caatacgctg attggtaaaa ctggtacacc
     6841 aagaccatag gaaagctttt ctagattgat tttttttcct tcttttttga tgacatccat
     6901 catattcaga ccaatgatca taggaatgtc taaatccaat aactgcgtag ttaaataaag
     6961 atttcgttct aaattggtag catcaataat gttgatgatc agttcaggct gttcctgaac
     7021 caaataatct cttgcaatca cttcttctgg tgtataagga gacaaagaat agatgccggg
     7081 taggtcctga atatgaatgt tcggattttt tttcaacgat ccttcttttt tttctactgt
     7141 tacgcccggc caattcccta catattgatt cgaaccagtc aagagattga acaagctagt
     7201 tttcccgctg tttggatttc cgactaacgc gatattcatt ctttttcctc cctttgtatc
     7261 aagattagtt cggcctctga ctttcgtaac gtcagttcat accctcgcac tgtcagttcg
     7321 attggatctc ccagaggggc tagttttcgg ataactattt ttacatttcg cgtcaatccc
     7381 atatccatta accgtcgctt cactgcgcct tgtccgtgga ttgctacgac gatcccagtc
     7441 tctcctgctt ttaattgatc aagagaagtc cagctttcag aggaagcagt ctttttattg
     7501 acaaagattt gacctagtat ttcccgactt actgccactc gatgactttt taccagtaaa
     7561 atggcattgt tgcctgataa attgacgacg acgattttac ttccggcaac gatcccgata
     7621 ttttgcaaat ggcgttttgt tttttcttct gtttcgattc gttcgacttg ataaacgaaa
     7681 ttctgctttg cctgcgctaa ggtctgcata ttgattcctc tcttgtgaaa taaatcatgt
     7741 attaaaatca ttcattatat gttctaattt tcttacgtgt tacgcaaata aatgagaatc
     7801 attctcacat agaatactct cggtaattga gaatgtcaat tatacttgtg atattatgag
     7861 cgaatcttta gtattgatag ataaaaatga gaaaaaaata tttcttcata gttttccaca
     7921 attttttctc tattaaattt gctattttcc cacacgatac ttgttttcag ctcttttttc
     7981 aaaaacacaa aacttagtat tccacagttt ccacaacaca atatgtagtg tttgttgttt
     8041 gactttttta ctaagagttt tatactatct attatagata ataagaatgt gttagaggag
     8101 gcactcacca tgaacgtaaa aatcttttct aaaaataatt gtatgcaatg taagatggtc
     8161 aaacggtttt taacagaaaa tcaaattgct tttgaagaag tcaatatcga tgaacagcct
     8221 gaagcagtag actggttgaa agcacaagga ttccaaagtg ttcctgtcat tacttctgat
     8281 gcagaaacag ttatcggatt ccgtcctgat cagttgcgta aacttgccag ctaacatgac
     8341 taaaagaatc aaaacgtgat acatggattt gaagggcttg agacagcacc agtttttgtc
     8401 gcaaacttcc aaatcctttt ttaaagctta aaaacaatgc aatgatttgc aatcaagcat
     8461 catcaaatac gaaatgaagg ttgggcgtac gtatgagtct taaaaattta caagatgtca
     8521 gttatttcaa gctgaataat gaaatcaatc ggccagtcaa tggtcaaatc cctttgaata
     8581 aggataaaga agctttagtt gccttttttg aggaaaatgt caaaccaaat acgatgactt
     8641 ttccatctat tatggaaaaa atcaattact tgatcgatca agaatatata gaagcagaat
     8701 tcattcaatt gtattcacca tcatttattg aaaagctata tcaatttttg gacgagcaac
     8761 atttttcttt caaatctttt atggctgctt acaaatttta ttctcaatat gcgttgaaaa
     8821 ccaacgacgg ttccgcttat ctggaaactt atgaagatcg agtagccttc aatgcgcttt
     8881 actttgcgaa cggggatgaa gaattagcac ttgctcttgc agacgaaatg atccatcaac
     8941 gatatcagcc agctacacct tctttcttga atgctggacg taaacgtcgg ggcgaattgg
     9001 tgtcttgttt cttgactcaa gtcactgacg atatgaactc tatcgggcgc tcaatcaact
     9061 ctgctttgca attgtcccgt attggtggcg gcgtagggat tactttatca aatcttcgtg
     9121 aagccggcgc tcctatcaaa ggatatgagg gtgctgcaag tggtgtcgtt ccagtcatga
     9181 aattattcga agatagtttc agctattcta atcaattagg tcagcgtcaa ggagctggcg
     9241 tggtttattt gaacgttttc catccagaca ttattgcttt cctttcagca aaaaaagaaa
     9301 atgcagatga aaagattcgc gtaaaaactc tttctcttgg cgtgatcgta cctgataaat
     9361 tttatgaatt gacacgtaaa aacgaagata tgtatttatt tagtccatat agtgtcgaaa
     9421 aagaatatgg tgtcccattc tcttatgtaa atattacagc tgagtatgat catctagttg
     9481 caaatccaaa cattcgcaaa caaaaaatca aagcgcgtga tcttgaaaat gaaatctcaa
     9541 aattacaaca agaatctggc tatccttacg taatcaatat tgatacagcg aaccgcgaga
     9601 atccaatcga tggcaagatc atcatgagta atttatgttc agagatttta caagttcaaa
     9661 cgccttctgt gattaacggc aaacaggaat atgaagtatt aggaacagat atctcatgta
     9721 acttaggctc gacaaatatc gtgaatttga tgcaaagccc taactttggt caatctgtgc
     9781 gtacgatgac tcgtgcattg acatttatta cagatgcatc tgatatcgat gtcgtaccac
     9841 cgatccaaaa cggaaataag ctgaatcata cgattggatt aggtgccatg ggcttacaca
     9901 cttatcttgc aaaagaacaa atggaatatg gctcagagga ttcattagac tttacaaata
     9961 tttacttcat gctgttgaat tattggacac ttgttgaaag caacaatatc gctcgtgaaa
    10021 gaaaacaagt tttccataat tttgaaaaat cagcttatgc tgacggcact tatttcgaaa
    10081 aatacgtaac tggagaattc cagccaaaat cagaaaaagt caaagcattg tttgaaggca
    10141 tttttatccc aactgctgaa gattggaatg ctttgaaaca agcagtaatg aaagatggac
    10201 tgtatcatca aaatcgcttg gcagtagcac caaatggctc aatttcatat atcaacgata
    10261 cgagtgcaag tattcatcct atcactcgca tgatcgaaga acgccaagag aagaaaatcg
    10321 gtaaaattta ttacccagcg ccttatctat ccaatgagac gattccgtat tacacttctg
    10381 cttatgatat ggacatgcgt aaagtaatcg atgtttatgc aacggcacaa caacacgtcg
    10441 atcaaggcat gagtttgact ttattcatgc gttctgaaat cccagaaggc atttatgaat
    10501 ggaagacaac aacgaagcaa acgacacgtg atttgaatat tttaagacat tacgctttcc
    10561 acaaaggaat caaatccatt tattacgtac gtacctttac tgatgatgca gaagaaatcg
    10621 gaagcaatca atgtgaaagc tgcgtgatct aatatcatag atagattcgc ttattcatat
    10681 tttaattaaa ggagcattct catgtcagaa acatattatg aagcaataaa ctggaatgaa
    10741 attgaagata tcattgataa atccacttgg gagaagctga cagagcaatt ttggctagat
    10801 acacgtatcc cactttcaaa tgacctagat gattggcgta cattgtctca attggaaaaa
    10861 gatacagtag gacatgtatt tggcggtttg acactgctag atactgtcca atctgaaagt
    10921 ggcatggatc agttgcgtaa agatgtccgc actccccatg aagaagccgt tttgaacaat
    10981 attcaattca tggaatccgt tcatgccaaa agttattctt ctatttttag tacactgaat
    11041 acgaaaaaag aaatcgaaga aatttttgac tggacaaata cgaatcctta tctgcaaaaa
    11101 aaagctgagc gtatcaatga aatctacaaa cacggtacac cgctagaaaa gaaaattgcc
    11161 agcgtctttt tagaaacatt cttgttctat tcaggttttt atactcctct ttattattta
    11221 ggaaacaaca aattggctaa cgtagcagag atcatcaaac taatcatccg agatgaatct
    11281 gtacatggaa cctacatcgg ttacaaattc caattaggat tcaacgagtt acctgaagca
    11341 gaacaagaaa cgttaaaaga ctggatgtac aatcttcttt atgagcttta cgaaaacgaa
    11401 gaacgttata cagaagaatt atacgatcca atcggctgga cagaagaagt caagacattc
    11461 ctacgctata atgccaacaa agcattgatg aacctaggga tggatccact ctttccagat
    11521 actgccaatg atgtcaaccc aatcgtaatg aacgggattt caacaggcac aagcaaccat
    11581 gacttcttct cacaagtcgg aaacggctat ttgttaggtc atgtagaagc gatgaaagac
    11641 gacgattatt tgattggatt ggaataagga tttattttaa ttcaaaaatt tttcgaaaaa
    11701 taaaaagagt ttagcgtaca gatattttaa atgtacactg aactcttttt tatgcttatt
    11761 cacttgtttc ttccagcact tttggactgt ctaacttttc cgaaacatgt tccattcttc
    11821 tcaataaata gattttccgg atatttaata ttatcgtctt aatcactgca tagacaggta
    11881 tgcaaatcag catgcctaat attcctgcga cacttcctgc tccaatcaat accaagatga
    11941 tcgtcaaagg atggatattc attgatttcc caaataacaa cggcttgatc aaatttccat
    12001 ctaattgttg gatcactaaa atcgacaacg ccatatacaa agcttgaaat ggagagataa
    12061 acaaaccgac aatgattgct ggtaccgctc caataaaagg gccgacatac gggataatat
    12121 tcgtgattcc gcaaatgacc gcaagcaaca agctataagg ctgatcgaat attctcattg
    12181 ccaaaaagct cattacacca ataatcaaag catctaagat cgtactgctg atataggcag
    12241 ataatgtttc gtttaattct tttaccgttt ggcgtaactc tttacgaatc gctttcggaa
    12301 agaagtgaga caacgcatca ataaatttgt gcccatcctt gaacataaat agcaaaataa
    12361 atggaacggt aaataacaaa ataaaaaatt gcatcataaa tgaaatgatt tttgagacac
    12421 ttgcagataa actgactaat atgatattga acaaattact aagagataaa tttgctgatg
    12481 ctaattgttc ttccaaattg aaatttttaa actcttcccg ctgcgccaat tcatttaacc
    12541 atttgcttgt ttcatctgca tacattggca agctctgggt caattgaatt gtctgttcca
    12601 ttaactgagg cactacattc atgacagtta aaacaactaa aatgattacg accaaaaacg
    12661 tcaataaaaa accgaacact ctcgggatcc gatgcttttc gagaaaaaca acgattggat
    12721 cgaacatata atagataaaa ccagcaacaa gcaatggtaa aaaaattgcc gaaagcattt
    12781 tcaatattgg actaaaaata ttcggcattt gtaaaataaa gtacacgccg atgatcgtga
    12841 gtactagctc cactgtccag aaaaataatt ttgattgttt aaaacgtgat tgcatacatc
    12901 tcctccaagg tcctttgata cttctattct atcatttaac aaagagttac gcgctgagat
    12961 tttcatttgc ttaaaaaaaa caaaaaaaat aaacatattt taatgagtag acgagtactt
    13021 aaacaaaaac acggtttgga gtagtgacaa aaattatact gcagtccctt tttccaaata
    13081 aacggtgttc aaaagcttgc atcttcgtaa taagcccagc caaacgaaaa atataaggaa
    13141 ctatttttat ttggctgggc ttattacttg atgtaggaca tctattttac aacctcagat
    13201 catttattgc gcaatactga ctaaatgcaa ctttgtgata tttttgtgta cggacaactc
    13261 ctcgatcaca tcggcataag tgagcccttt tggaagatca atcgtataga tatttttgta
    13321 cactcggtag tcatcaacga attttacgtc aaaattcaca tcttcgatta cgatattttt
    13381 ctcttcaaaa taatcggtaa gaaaatcttt ggtttctttt ctatggataa attggatttc
    13441 taacttttta gtcgtaggta catggatcac gcgtttcact actgtgagtg caatagcaat
    13501 cccgataaaa ctgatgatcg caatggaata gtaacccatg ccgatggcga tccctaatcc
    13561 agcaattgcc cataaagaag cggcagtcgt aagtcccgtc accgattgtt tcgtgacgat
    13621 gatcgttccc gcgcctaaga aaccgacacc actcaccact tgagcaatca gccttgcttc
    13681 atctgaacga atcgttccag caagctgtgg atactctaaa gcatctctta atgcaccact
    13741 agcgatctca acttggatca atgcaatgat acatgctccc atacaaacta aaatatgcgt
    13801 acgcatacct gctggacgat ttttatattg tcgttcaaaa ccgattgctc cgccaaatag
    13861 catagcgatc cctaaacgta aaataatctc tggaattgtc aataccatat cattcatcta
    13921 aacaccctcc agtccaatat atcatgtctg aaaggagcag acaaaaaaag tgcaattttt
    13981 acctcttacg aaaaaaggac tgtgacaaaa tttttgtcat agtccttttt atgaataaac
    14041 ggtgttcaat cagctgaccc tcggtattac tcaacaaacg gcaaaatatg gggagctatt
    14101 tttcctttgt cttctctaat actcaggtca aaacgctgat ttcacaacct ctttagccta
    14161 ctattgaaaa ttacaactta cttgctgctt cttcatcgat gatcacaaca acattgtcgt
    14221 gattttgcaa tacacttgct ggcaaatgat tcgttacagg tccttcaatc atccctttga
    14281 ttgcatcagc tttgctttcg ccaaatgcca tcaagacgat ttctttactt ttcataattg
    14341 aaccgattcc cattgaaaca gcttttgttg gtacatcttc tgctttatcg aagaatcgtt
    14401 tgtttgcatt gattgttgat tctgttaagt caactactga agtcgttccg tcaaatgaag
    14461 caccaggttc attgaaaccg atatgtccat tttgcccgat tcctaagatt tggatatcga
    14521 ttggatgtgc atcgatgatt tgatcatact ttttgcatgc tgcttccaga tcttctgctt
    14581 ttccatctgg tacaaacgtt tctttgaatg gttttttatc aaataattgg acatccatga
    14641 aatgacggta gctttgttca tctgaaccgc ctagtccaac gtactcatct aagtttacag
    14701 aagtcatttc actgaaatcc aaatcgcttg aagtcatttc tttatataaa gtaactggtg
    14761 tgcttcctgt tgccaatcct aatacttttg cacctttgtt cataccttct ttgatgattt
    14821 caaatgcttt ttttccgcct tcttcagcgt tttttacacg aatgatttcc atagtgattg
    14881 ctcctttata ttttggtata gcccaattat agcattcttt ttttcttttt acaataatta
    14941 gtatagacca aattgcattt ttttcatttt cttgacaata aataggaatc cgtgttaggt
    15001 ttcataagat ggttcaatca aacaatccat gttgcatgaa aaccaagcaa gtgattcact
    15061 gtggacgctt tatgtctgca gtttttttat gaaaaaggag ttattatgag tatactgaca
    15121 ttaaacaaca ttacccagca atttggcgac aaaatcctct atgaaggtgt cgatctgcaa
    15181 ttaaatgctg gcgaacatct tgggctgatt ggacaaaacg gagccggtaa aagtacattg
    15241 attaaaatca tcactggtga gatcttacca gatgacggac agatccattg gcaaaaaaat
    15301 attcacaaag gttacctcga tcaatatgtg gaagtcgatg aaacattaac aatcgaagaa
    15361 tttctaaaaa ctgcgtacgc aaaagaatac gaaaaggaag aaaaaattgc ttttctttac
    15421 caaacatata gcgagacctt cgatgattcc ttgctggaaa aagctgggaa actccaaacg
    15481 gaattagacc aaggagtgtt ttatcaaatc gacaccttag ttgctgagat gagcagcggg
    15541 ttggggatcg atgtcttagg tttagattca ctgttgaaaa atctaagtgg tggacaacgt
    15601 tcaaaggtga ttttagcaaa actactttta gaaaacccag atgttctgat cttagatgaa
    15661 ccaaccaatc acttagatga tcagcatatc caatggctga cgcagtttct tcaggatttt
    15721 gacggtgcgt atatcgtgat ttcccatgac caagaatttc ttgatcagat caccacccat
    15781 atcgcagaca tcgagtttgg aaagattact aagtatactg gccatctaaa acaagcattg
    15841 aaacaaaagg aacaaaatcg cgaaagctat ttgcgccaat atcatgctca acaaaaacaa
    15901 atcgaaaaga ccgaagctta tattcgaaaa tacaaagccg gttcccgatc aacgatggca
    15961 aaaagccgac aaaaacaatt agataaaatc gaacggctga cgcctccagc ttcagcaagt
    16021 aaagctgtct ttgattttcc attctcgcct attgtcacga cattagcagt ggaaacaact
    16081 gatttagtca tcggatatga tcgaccgcta cttgcaccca tcaatttgac catccgcttt
    16141 ggtgaaaata ttgccattcg aggcttcaac gggattggaa aatctacttt attaaaaaca
    16201 ctgatcggtg agattgaaaa aataagtggc gatttccact tcccagataa cacaaaaatc
    16261 aattactttt cacaagatct gtactgggaa aatccattag aaacaccact acaatattta
    16321 agcagtcaat ttccaaaagc gactatcaaa gaattacgtc gccaactagc taaagcaggg
    16381 ttagttaatc aattagcaag tgaacctctc actacattaa gtggtgggga acaaactaag
    16441 gtgaaacttg cacagttgac aatgaaccaa ggtaatttac tgattctcga tgaaccgacg
    16501 aaccatatcg atcaagaaac gaaagaaagc ttacaagacg ggatacagaa atttccagga
    16561 acagtaatca tcgtctctca tgaacaagaa ttttatcaag atcttgtgca acgagtcatt
    16621 gagattgaac caaaataaac gtttgattga atagttgaaa aaaaggagct gtgacaaaag
    16681 ttttgtcaca actccttctt ctgaataaat ggtggccaat tagctgacct tcggtattag
    16741 ctcaaaaaac ggcaaaatat gaggagctat ttttatttgg ctattcttat tacttgatgc
    16801 agaacagctt ttcacaacct cttttcgtct attttttatt ttcgagattt ctctttcctt
    16861 taaaaacaac tgtttgaaag aagaacgcac atccttgaaa aacaagcccg ctaaccgtta
    16921 cacctaacca gccatactgc tgccacattg tcgtccctag caatgaacca gccgcacctc
    16981 caataaagta cataaacata aataca
//
LOCUS       DACEUZ010000053        16631 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.053, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000053 DACEUZ010000000
VERSION     DACEUZ010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16631
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.053"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(45..779)
                     /locus_tag="IXG44_001467"
     CDS             complement(45..779)
                     /locus_tag="IXG44_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAQ1658193.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRFVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1105..1515
                     /locus_tag="IXG44_001468"
     CDS             1105..1515
                     /locus_tag="IXG44_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HAQ1658194.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     EGKTAEGVEALLGYVRKKKAGRVSVNEMEENQID"
     gene            1508..2209
                     /locus_tag="IXG44_001469"
     CDS             1508..2209
                     /locus_tag="IXG44_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HAQ1658195.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2209..3822
                     /locus_tag="IXG44_001470"
     CDS             2209..3822
                     /locus_tag="IXG44_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ1658196.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKKTIKTITKKEKKDDI"
     gene            3812..4033
                     /locus_tag="IXG44_001471"
     CDS             3812..4033
                     /locus_tag="IXG44_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HAQ1658197.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4030..4791
                     /locus_tag="IXG44_001472"
     CDS             4030..4791
                     /locus_tag="IXG44_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HAQ1658198.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEELSLRFPETFAYALGDVRQMKELQQAVSKCQDRFGEVTILLNTSGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRRKSGTIINMASIAGLVAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5172..5681
                     /locus_tag="IXG44_001473"
     CDS             5172..5681
                     /locus_tag="IXG44_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAQ1658199.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANMASPLGLIDVVVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5751..6290)
                     /locus_tag="IXG44_001474"
     CDS             complement(5751..6290)
                     /locus_tag="IXG44_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HAQ1658200.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVVVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6430..7041)
                     /gene="rpsD"
                     /locus_tag="IXG44_001475"
     CDS             complement(6430..7041)
                     /gene="rpsD"
                     /locus_tag="IXG44_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HAQ1658201.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7357..8154
                     /locus_tag="IXG44_001476"
     CDS             7357..8154
                     /locus_tag="IXG44_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-nitrite transporter"
                     /protein_id="HAQ1658202.1"
                     /translation="MNPVSPLFEKIDASIEKKANLFENSFSRYAVRAVLACLFLTLGT
                     AVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAELGTSNMLYMTVGVFRQRLA
                     LSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNFMLESIAVKLAKPAATILV
                     EGMFANIVVNTAVLISMRMKEDAGKVITVVFIIFIFAFLGYEHVIANFPAFTLAYFAS
                     QGQTDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQSKSSYVD"
     gene            8182..8817
                     /locus_tag="IXG44_001477"
     CDS             8182..8817
                     /locus_tag="IXG44_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HAQ1658203.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVYYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8837..9610
                     /locus_tag="IXG44_001478"
     CDS             8837..9610
                     /locus_tag="IXG44_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ1658204.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10018..10815
                     /locus_tag="IXG44_001479"
     CDS             10018..10815
                     /locus_tag="IXG44_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HAQ1658205.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKKIQLIQINDDRPFME"
     gene            10793..11206
                     /locus_tag="IXG44_001480"
     CDS             10793..11206
                     /locus_tag="IXG44_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1658206.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEQEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11190..13835
                     /gene="mutS"
                     /locus_tag="IXG44_001481"
     CDS             11190..13835
                     /gene="mutS"
                     /locus_tag="IXG44_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAQ1658207.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLKKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKNSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13853..15961
                     /gene="mutL"
                     /locus_tag="IXG44_001482"
     CDS             13853..15961
                     /gene="mutL"
                     /locus_tag="IXG44_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HAQ1658208.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEEPKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPVV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15983..16543
                     /locus_tag="IXG44_001483"
     CDS             15983..16543
                     /locus_tag="IXG44_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HAQ1658209.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARIVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
BASE COUNT         5399 a         2969 c         3602 g         4661 t
ORIGIN      
        1 ttggtacgca atcgctgtac caagtttttt tatttagctt ttgattaagc cataaataat
       61 aatttatata agaatactgc tacgacacca gcacagatag gtgctacaac tggtacccat
      121 gcatatcccc attgagaaga acctttatgt ttcaatggaa gaagtgcgtg tacaaaacgt
      181 ggagcaaagt cgcgggctgg gtttagtcca ggacctgtag gaccaccaag tgaagcaact
      241 aatgtccata caaggaaacc gagtgctaag tgagctgtac caagattatc ttggaagaat
      301 ggtgcttttg taatacctaa ggcaccaaag aataaaacga atgaaccaaa gaattcattg
      361 acgaaaccat tgaatttaga accaacgctg ttgattgttg agaatgtacc taacacatgg
      421 ttcacgtctt ctgtttggtc gtagtaaggt ttgtaacaag ctactacgat caattgtcca
      481 atcgctgcac caatcaattg tgcaatgata taaggaacaa cttcagccca agggaataga
      541 ccattagatg ctaaagcaat tgtaaatgct gggttgatgt ggttaccgct gattccacca
      601 aaaatcattg ctggcatcat aaccccgaat ccatacccga cagcaatcag catccagtca
      661 ctgccatatc cttttgtacc ttttaaatct acgtttgcta cagcaccgtt acctagaacg
      721 attagtaatg ctgtaccaat catttccgcc gcaagacggg tcattagtga gtaatccata
      781 aatacctcca aaagttataa aaatgcaaaa aaaaagaaac atcaacacga aaagcatcgg
      841 tgcagtgttt cgtgtcggaa ggatttctgc agtcttaaca aatgtgaata aaaaaatgaa
      901 ttccattact tataagaaac cagaccaaca gtaaaactcc aactcttttt aaaagttctc
      961 cacaaaccac caagcttaac tataaatata ccaaaaaaaa aacgtttttt ttctcaaaaa
     1021 ttgtatctta gaaacagcat gttttgaaac tttcagaaaa ggtttgttag taaaatgaaa
     1081 gctatacgta aggagtgttg tttgatgaaa ttttttcaag agtttttcat tattattttc
     1141 ttttctgtat taggagaagg catacggatt tttgctcatt tacctattcc tggaagtatc
     1201 ttaggtattc tttttttatt tttagcattt gaaatgcatc ttgttgatcc tgaaaaaatc
     1261 ggagcaactg gtgatttttt attgaataat ctagctattt tatttgttcc tgctggtgta
     1321 ggtttattgg aatattttga tgatattgct tcgatatggc ctgttttact gggagcagtc
     1381 gtcgtgtgtt ccatcgtcac gatggttgca gagggaaaaa cggcagaagg tgtagaagct
     1441 ttgctgggct atgtccgtaa gaaaaaagca ggaagggtat ctgtaaacga aatggaggaa
     1501 aatcaaattg actgagtttt ttgtgaatcc actatttggc ttaagtttga ctttaggagt
     1561 gtatgtatta tctgataaat ttttgcggaa atataaactg cctgttttga atcctctcgt
     1621 attatcgatt gttgttatta tcttattttt gcatttttct gggatcagct ataaagacta
     1681 taatatcggg ggcaattttt tgactatgct gattactcct gcgacagtgg cacttgccat
     1741 ccctctttat aaaaacttcc gtttactgaa agagaatttt tatcctgtaa tggctgcgat
     1801 tttagcagga atagtcgcaa attgcttggt tagtgtaggg ttcggttact tcttctctct
     1861 tcataaagag atggtgatct cattactacc taaatctgta acgacggcta tttctgtcga
     1921 tttatctcat acgatgggcg gggtgaatgc cgtgacactt gctattgttg tctctacagg
     1981 tatttttggt tcgctgatcg ccactcatat tttccgccta ttcaagatcg aaagtccagt
     2041 tgctcgcggt gtggcgttag gaagtacttc tcatgctatc ggtacagcaa aagcaatcga
     2101 gattggcgaa atagaaggaa tcatttccgg acttgcgata tgcgtaaatg ggatattgac
     2161 cgttttgttg ctgccgttat tctttcagcc ttttgctggc ttgttttgat gcgaaaacca
     2221 ctattttatg ctacaataac caaaacgttc gagcataaaa aaggagggaa agacgtgtat
     2281 ttgtcttccg agtatcttaa acaatttgat aaagatctat attataagat tctcctccta
     2341 gaaagttttg aggatcaagc ttggcatacc gctgcacaat tagcgcaagt tgttcagctg
     2401 gatgcccgca gcgtgtcgaa atacctgaac gagctgtcta aaaactatca gcagtttagc
     2461 ggtaagacac accctttgtt tacaaaaaat caccgttctg gttataactt ttatgatacg
     2521 ctcgattcaa ttgaacatga gcgttttttg atctatctcg tgcaaagtac gctgaaattc
     2581 cagttgctgc acgacatttt ttttgaagaa ttccatacaa tgtaccaatt tgcgcaaaag
     2641 cattacatta gtgaatctac tgctcatcga aaaatcaatg aatggaaaca gcaactgcaa
     2701 acttacggaa ttcggctgca acgaggtaca tatatcgctc aaggcgaaga agaaatcatt
     2761 cgactttact tgcatatgac attctggcag ttgtttcgag ggaaaatctg gccatttgaa
     2821 acgatttctc aaatggacgt aaagaatatg gcagaacata tcatggcttt ttttaatgtt
     2881 cgtctgaacg agatcaaaaa aagacgattg gaatacatgc taggtgcctt ttttttaaga
     2941 aaatcacaga agcattatgt tgtattgaat gagaaaaaaa gacggctgat ttctgacaat
     3001 ctgttgttcc agcgattttg ccaagtcatg gagccggtat ttcctaatta ttttcaagta
     3061 gaagacgagt taggcgcatt atttcttgta ctgatgactc gtgaagaata ttatagtgat
     3121 cctaagatac ggaagaaaat ttttgatttt catcaacaag caaaaacacc gccctttaca
     3181 gccctttcag aagcaaaagc cgctctttct ctttatcagg aagagcaggg gctgccagca
     3241 gaaaatctaa ccttcgaggc agaaaactat ttattcagca gtcatttttt cgcctatctt
     3301 tttcctaatg cgaaagaaac gattgatggc aatagtagtg attttattaa tcatttagtt
     3361 attgaaaaca aggaattgaa acaatggctc gtacattttt ttgaaagtag acacaaacat
     3421 cccaatcatc tggcatttaa aaatcatgcg tttttgatgg gtcggtatct gactgtgttt
     3481 aaaacattag gagcctttac tccacagctt ccaaagatca cgatcctttt aatgacagat
     3541 ttcccattat ttgaagagca actgttagag gagggattac ggacattttt ccgtaatgag
     3601 tatcagctga tttttctgcc aacagattat cgggggagag aagttgatct tctgatttcg
     3661 acaagtaaag tccaccgcaa accatgggcg gatcttgact attttattgt gaccgaagaa
     3721 ctaaaattaa ttgattacat ccaactatct caaaaatttg aaatgattca aaaaaaaact
     3781 attaaaacaa taacaaagaa ggagaaaaaa gatgacattt gaagaaattt taccagaatt
     3841 gaaaaaaggt gcaaagatta ttcgtaaagg atggagtgga tttgaacttt atgttgttct
     3901 cgtatccgat gatgaatttg atggctgtcc agtgacacct tacttattga tcaaaacaag
     3961 tgacgaaggt ttttccagct tcgctcctac agtttgtgat attttagcag atgattgggc
     4021 gatcgtagaa tgaaaaacca gtatccagat ctagctaaac aggttgtatt tatcacagga
     4081 gctgcttctg gaattggtgc tgcgcaagcc cgagcgtttc ttgaacaaga tgcttttgta
     4141 tttggattag atcaaaagtt cggtaaaatg gaagaactct ctttgcgatt tcctgaaact
     4201 tttgcctacg cactaggtga tgttcgacaa atgaaagaac tccaacaagc ggtatcgaaa
     4261 tgccaggatc gttttggtga agttacgatt ttactgaata catcggggat attggatgcc
     4321 tacaaaccat tgatggaaac tgacgaatcg ctgtgggatc ttatttatga gacgaatgtg
     4381 aaaagcatgt atcagctgac aaaaatcatc cttcctgata tgatccgccg aaaaagcgga
     4441 acaatcatca acatggcttc gattgcgggt ttggttgctg gcggaggtgg gatcgcttat
     4501 acaagtgcga aacatgcaat cgtcggattt accaaacaac tcgctctgga ttacgcagga
     4561 cagggtatcc gagtaaaagg aattgctcca ggagccatcc aaacaccaat gaatgcggct
     4621 gattttgaag ggaaaggtga gatggctgag tgggtcgcaa atgaaacacc agtcaagcga
     4681 tgggcacagc cggaagaagt agctgagctg acgttatttc ttgcctctcc tcaagcaagc
     4741 tacatacaag gaacgatcat cccaatcgat ggcggatggt tgttaaaata accttgtgtt
     4801 tttttcaagc ttctgtatga taaaactaaa gagagaaaca acggttgatg ataaaagaaa
     4861 gtcgatgact gttcccgaca ttcgatgccg aacagccagt tcaaaaaatt ctttttaata
     4921 atcgttaata ttcgaaacta agcggctttt cacgaccttt tgatataaac ggtgttcaaa
     4981 agcttgcaac tgcggtaaca agtccagcca aatgaaaaat ataaggagct atttttattt
     5041 gactattctt attacttgtt gcagggcagc tttttcacta ccttctgcgg cacttcagtt
     5101 ggaattttct tcccatcctt tggtcgcagt cattaccaag ataaaattat cttgcaaagg
     5161 agaaatagaa catggaaaaa aataataccc gttcagccaa acgctggaca acgacagatg
     5221 tgactcggat ggcgctagtc actggtttat atgtggcagt gacgctggtt ttgtctgtct
     5281 ttagctttgg agtgattcag attcgtctct cggagatgtt caactatctg tccttgtata
     5341 acaaaagata catcgtcgcg gtaacagcgg gagtagcttt ggctaatatg gcttctcctc
     5401 ttggactgat agatgttgta gtaggaagcg tcagcacgct gcttgtattg cttatcaact
     5461 ataaaatcac tagtcgcatc aaaaacatga aaatcaaaat ggctgtgaca gcatgcgtat
     5521 ttgctttttc catgtttaca gtagctggtc agctaacgat tttatatcaa ctaccttttt
     5581 tcttgaattg gtgggtgatt gctttaggag aattggcatc aatgattgtc ggtgggataa
     5641 ttatttattg gatcggtaaa aaagtggatt tgtcaaaata aagatctgtt taatttaaaa
     5701 tatggcagcg tggaagagac tttgagtctt cttccatgct gcttttttga ttaatttgta
     5761 tgaagaaatc gatttagccg ttttttcatc aagataccga tataagctgc tccagttgcc
     5821 ttgattgctt ccggcagtaa aaacggcagt agtccactgg caaatgcagt tgtaaaagta
     5881 agatcaccac ttattttcag ccagatcgtt ccgcagatca atgcagcaag agagcccatt
     5941 agattggcaa agattgcata taagtaatga gcatccgttc tttcgatcat ccaaccagtt
     6001 atccacgttt gtaagagaaa tccaatcaaa tatccgccag ttggcccaaa taaaacagct
     6061 atacctccag tcatccctga aaatacagga agaccaatca agccaagtaa aagataaact
     6121 acaacagata tcaatcctag ttttcttccg agaaaagttg ctgttaatcc aatagcaaat
     6181 gtttgccccg tcaaaggaac caagccaaaa ggaatggtca tctgagaaag gacagaaata
     6241 atcgcggcaa attccgcagc aagtattaat tctttcaatg ttcggttcat aatgccctct
     6301 tttcgttaac ttaatatttt tatttggtta acgaaattgt aaaacaaagt gattactttg
     6361 taaatagaaa atataaaaaa agaccagcta tccaatagat agcggtcttt ttcaagaaag
     6421 cttttgaaat tataagcttt ggttgtagta ttcaacgaca agagcttcgt cgatttctgg
     6481 atatagttcg tcacgttctg gtaaacgagt caatgaacct tccaattttt cagcgtcaaa
     6541 gcttacgaat gctgggcgtc caacagttgc ttctacagct tcttttactg ttacgatgtt
     6601 ttgtgatttt tcacgaactg agatcacttg acccacttct acgtggtatg aagggatatc
     6661 aacgcgtttg ccatcaacta aaatgtgacc atggttaact aattgacgtg cttgacgacg
     6721 agtcgttgca agtcctaaac ggtaaacaac attgtctaaa cgttgttcta gtaagatcat
     6781 gaagttaaca ccatgtttac cttctttgat tttgctagct tttacgaaca agttacggaa
     6841 ttgacgttcg ttcattccgt acatgtgacg aagtttttgt ttttcagtta attgcatgcc
     6901 gtattctgat tgtttgccac ggctgttagg tccatgttga cctggtgcgt aagggcgacg
     6961 tgctaattct tttcctgtgc ctgacaatga gatgcctaag cgacgagaaa ttttccatga
     7021 tggtcctgta taacgagaca ttaaaatttc ctccaatgaa tataagtttt tggagtaaaa
     7081 taatccgttg aaaaattcat aatcatatag tttgttcttc aatcttcacc tttgcagccg
     7141 cggttacaca atcgaacccg agacggggca acaaactagg gatgagttat tatttttctg
     7201 ctgcattatt ttacacaaac tacagtatat gatagatcat ccacgaatgt caagataatt
     7261 agtacataaa aacgctttac aaacctaggc tgtctttata aaatgagagg gcatgtgcaa
     7321 atggaaaaaa gcgaaggaag tttaaggaga aaatcaatga atccggtatc accattattt
     7381 gagaaaatcg atgcttcaat tgaaaagaag gcgaatcttt ttgaaaacag cttttcacgt
     7441 tacgctgttc gagcggttct cgcctgtttg tttttgactt taggaacggc tgtggcattt
     7501 gcaatcgcga tgaaggggga agatatcgcg catggtctag gaaagatttt atacgcattt
     7561 atgttcagct ggggtctggt catgattcta tacatgaacg ctgaattagg aacatcgaat
     7621 atgctttata tgaccgtggg tgtttttcgg cagagacttg ctttaagtaa ggcagccaag
     7681 attctatttg cctgtatttt tttcaatatg attggcggta tattatgtgg atttcttatt
     7741 tctatgacta cgccatttca agatttaacg ttggacaact ttatgttaga atcaatagca
     7801 gtaaaactag caaaacctgc agcaacgatt ttggtggaag ggatgtttgc gaatatcgtt
     7861 gtcaataccg ctgtactgat cagcatgcgg atgaaagagg atgccgggaa agtgatcaca
     7921 gtcgtattca tcattttcat ttttgctttt ttaggatatg aacacgtgat agcgaatttt
     7981 cctgccttta cgcttgctta ctttgcttct caaggacaga cggatggaat gacagtcagc
     8041 aatgtgttac ataatttgtt ttttgctttg gcaggaaatt atattggtgg cggtttagta
     8101 atgggactgg gatatgcttg gctgaatcag tcaaaatcat cctatgtgga ctaaaaagtc
     8161 gttcattaaa ggggaagtgc catggaagtt ttagtttttc caaaaaatga ggaaaaagcg
     8221 ttacaaatgg aaaagtacat gaaaaatctt ttttcctttg ctggagtacc taagccggaa
     8281 cgcgctcttt tacaacaacc gttactggat gagagcaaaa atttatctat ttctgaagtt
     8341 ttagcttttg tttattatta ttatgaacaa atcgaaagag agtatcagta tgtagcgatc
     8401 gatttagtcc agaaaaacat caaacggctt acatttgaag acttagtcgt attgttgcca
     8461 ctagttcaga aaaaagaatg gtgggacagc atcgactctt ggagaaaagt atacagcaca
     8521 tggtgtaaag cctacccaga gcagcgtgaa gaagtctttc aattgtttga aaaaaaggaa
     8581 gatttttggc tacgtcgaat agcaatcacg ttgcaattag gttataaaca gcaaacagac
     8641 acaggactat tgatccgagc aatagacgaa gatcgtcaaa caaaggaatt ctttatccaa
     8701 aaagcaattg gttgggcatt acgcgattac agtaagaccg atgccgattg ggtaaaaaga
     8761 cagctgacaa cagatttgag taagttagct gttcgagagg caagtaagta tttataagaa
     8821 agcgagacga caagagatga gacgcaaatt atttgctttt gatattgatg gaacgttgtt
     8881 agattcaaac aaacaagctt tagatagcac gagagaagca ttggaaaaat tacgcaaagc
     8941 cggtcatctg gtcacgattg ctacagggcg gagccgattt catgcgcaag agatcattcg
     9001 cgatcttgat tttacgaact acattttatg taatggagcg gccggttttt tagaccatga
     9061 acaagtatat aagaatttac tagatcgcga ccagttggat gcgttcgttc aagaagcgca
     9121 tcatgagcag atcgatacag cttttgtcag cttagacaat atcaagcgtt cttcttccaa
     9181 tgatgtaacg gtgatggaag aagcgatgca ttctttcggt gctgtcttgc ctgaattaga
     9241 tcagtttttt gcagaggaac aagaaatata ccaagcgtta gcatttttcg atcaaacata
     9301 tgatgggaag ttctcttcat atgataaaat tcgatttgtc cgctggcatg agaacagtgt
     9361 agatgtcgta cctaaaaatg gttctaaagc agcgacaatt ctgcacttag cagaacaagt
     9421 aggcatccct catgaagaca tcatcagctt tggcgatgga caaaacgacc gtgaaatgtt
     9481 gcgcatgtca ggaattggga tagctatggg gaatgctgtg ccagaagtac aagcagaagc
     9541 taagatggtc actgatacaa acgatcagga tggaatttgg aaggcattga aagagttaag
     9601 cgtgatttaa aagaggttgt gaaaaagctg ttctgcatca agtaataagc ccagccaaac
     9661 aaaaatagct cctcctattt ttcgtttttc tgggcttatt accgcagatg caagcttttg
     9721 aacaccgttt aaggaagagg tcgtgacaga agtggtcagt ttcaagaaat aagaagggat
     9781 ttacgaaaat tgcttctcaa atttttgtaa aattacggct tatttctgaa gaagtagatg
     9841 aaacaacgtt tattcgggaa atgggactgt gacaagatgt ttgtcatagt cccatttttt
     9901 tattttattt ttcaaaacta agaaatacca ggaaagagtt ccttttgttt gaccttttct
     9961 ttcttattga aaacaactat aatatagagt gatgttaaaa gagaagagga atttagtgtg
    10021 cgggtattgt ttattggtga tgtagtaggt tcaatgggac gagagatgat tactgaatat
    10081 cttcctagat tgaagaagaa gtaccgtccg caagtaacga ttgtaaatgg tgagaatgcc
    10141 gcttctggac gtggaatcac agaaaaaatc tataaaaaat ttttgcaaga cggcgtagat
    10201 gtcgtgacga tggggaatca tacgtgggac aaccgcggaa tctttgagtt tgtaaatgaa
    10261 gctaaaaaga tggttcgtcc ggcaaatttt ccggaaggga cgcctggaca agggatggtc
    10321 tttgtcaaag tgaaccaagt cgaactggca gtgatcaaca tgcaggctcg ttcttttatg
    10381 gtggacttgg atgatccttt tcgtaaaatg aatgagctgg tagaagaagc cagaaaacgt
    10441 acgccgatca tttttgtaga ttttcatgga gaaacaacaa gtgaaaaaca agcaatgggc
    10501 tggtttttag atgggaaagt ttcagcagtg gtcggtacac atacacacgt tcagacaaat
    10561 gatgcgcgaa ttttgccaag aggaacagcg tatctgacag acgttgggat gacaggtccc
    10621 tatgatggta ttttgggtat gcgcagagaa cctgtaatcg aaaagtttct gaccgcatta
    10681 ccaaaacgtt ttgaagttgt ggaacaaggc cgatcgattt taagcggctg tatcttggaa
    10741 ttggatgata cgacaggtca tgcaaaaaaa atccagctga ttcagatcaa cgatgatcga
    10801 ccgttcatgg agtagaaaga gaggcagttt tttgcagcag gagccggaaa ttcaaaaaga
    10861 agtaaggaaa ctgacaaaac ttttaagaga gaatgaaacg atcattcgct acaaagaact
    10921 tgaagaaaag atccagcaaa accagtattt agctgagttg agagaaaaaa tcaaacaagc
    10981 gcaaaaagat gctgttcatt ttgcccacta tgacaaacca gcagcagaac aagaagcaat
    11041 caaacaagcg gatcaattca tgcaagaatt tgatcagcat ccgcttgttg tagcttatag
    11101 aaaacagctg ttggaagcag atgatctgct tcatcattta acaactatga tacaagaaga
    11161 aatcaatgga caaatagagg aggaaaaaca tgcctcaaaa aactaaaaat actcctatga
    11221 tggaacaata tctaagcatc aaggcccaat accaagatgc ttttttgttc tatcgtttag
    11281 gagactttta tgaactattt tacgaagatg cgatcaaagc gtcacagctt ttggaattaa
    11341 cgttgacgag cagaaatcga aatgcagaag aacctattcc tatgtgcggt gtacctcacc
    11401 atgcagcaca aggatatatc gatgccttga ttgaaaaagg ctataaagta gcgatttgcg
    11461 agcaggtaga agatccaaag acgactaaag ggatggtcaa gcgcgaagtg atccagctag
    11521 tcactcctgg tacagtcatg gacagcaaag gattgtcggc aaaagacaac aattttttaa
    11581 cagctgtagt tagtcaagga aatgaatttg gctttgctta tgccgattta agtacgggag
    11641 aattgaagac ggctcggttg catgatgaag aagctgtttt aaatgaagcc tctgcattgc
    11701 agacaaaaga actcgtatta ggcagtgaga tcactgatac ccttcgtgaa cagctaagcg
    11761 ggcgtttagg gctagtcttt tctcaacaaa atgagatgga agaaaacgcg gaatttagct
    11821 ttttgacaag cgaactagtc gatccgctga aaaaagaagc aaccggcaaa ctattgactt
    11881 atttatcggt gacacaaaag cggagtttag ctcatatcca aaaagcagta gaataccaac
    11941 cagatcattt tttgaaaatg gattattatt caaaattcaa cctagaatta agccaatcta
    12001 ttcggactgg gaaaaagcac ggcactcttc tatggttgtt agatgaaaca aaaacagcaa
    12061 tgggtggccg tttactaaaa caatggctag accgtccgct tatccaggca aaacaaatta
    12121 aagcaagaca agaaatggtc gcttctttgt tagacagcta ttttgaacgt atcgatttgc
    12181 agtcagcatt aacgaaagtc tatgatctgg aacgcctagc aggtcgagta gctttcggta
    12241 atgtcaatgg gcgagacttg atccagctga aaaattcttt agaacaagtg ccaatgatca
    12301 gagggttgat tgaaggaatc gatcaaggtc aatggcaaag tctgctttca gaaatgcagc
    12361 cgatggacca tctcgtccaa ttgatcgacc aagctatcca agatgatccg ccgttacaaa
    12421 taacggaagg aaacatcatc aaagatggat tcaatgagca gctcgatatg taccgttctg
    12481 ctatgagaaa cggaaagaaa tggttggcag aacttgaagc aaaagagcgt caagaaacag
    12541 gaatcaaaaa cttaaaaatc ggatttaacc gcgttttcgg ttattatata gaaatcacaa
    12601 aagcaaatct tggaaatgca gaactggaga agtatgaacg aaaacaaact ttagcaaatg
    12661 cagagcgttt catcacacca gaactaaaag aattggagac acaaatcctc gaagcagaag
    12721 aaaaatctgt tgatttagaa tatcagttgt ttttagctgt gagagaagaa gtcaaaaagg
    12781 ctatccagcc gctacaagta cttgcaaaag caatcagtgc agtagatgtg ctacaaagtt
    12841 ttgcaacgat cagcgaacgc tatcagtatg ttcggccaga acttgtcagt gacaagcatc
    12901 agcttctgat caaagacgga cgacatcctg tagtagaaaa agtattagga catcaggaat
    12961 atatccctaa cagcgtagaa atggcagaag acgaaatgat cttgctgatt acaggaccga
    13021 atatgtctgg taaaagtacg tatatgcgac agttggcatt aactgttctg atggcgcaaa
    13081 tgggctgttt cgttccggca aaacaagcag ttttacctat ttttgatcgg atttttaccc
    13141 gaattggtgc aagtgacgac ttaattgctg gacaaagtac ttttatggta gagatgatgg
    13201 aagccaatca agccctgcgt tatgctacgc caaacagttt gattttgttt gacgaactag
    13261 ggcgaggaac agcaacctat gacggaatgg cgttggccca agcaatcatc gaatatatcc
    13321 atcgtcatgt acaagcaaaa acactctttt ctactcatta tcatgaactg acggtacttg
    13381 agaaagaact tccgcagctt aaaaatgtcc atgttggagc cgttgaaaaa gacggagaag
    13441 ttgtcttctt gcataaaatg atggatggac ctgcggataa aagctacggc atacatgtgg
    13501 caaagatcgc aggattgcct tccaatctac tagaacgagc agcagatatc ctgcatacgc
    13561 tagaaaatgg cgataacgga caccatactg ttccagctga agtagaagag aaaactaaaa
    13621 cagccaaaca ggagcaaata aaaccacctg cagaaaaagt aaatcaagac catatacaag
    13681 accgtgtaaa agaagaaaac caacagcttt ctttattcag tgaattatcc gaaaatgaaa
    13741 cagaagtcat tgaatcactg aagcaaataa atttactaga aatgacgcca atggatgcac
    13801 taaacacatt atatgaatta caaaaacaac tgtaggaaga aggcggatat cgatggccaa
    13861 aatccaagaa ttatctgaac gtttagctaa tcagatcgct gcgggagaag tagtggagcg
    13921 accagcttcc gttgtgaaag aattagtgga aaatgcaatt gacgccggca gtactcagat
    13981 tgatatctta gttgaagaag cgggactgcg gacgatccaa gtgattgata acggagaagg
    14041 cattttggca gacgacgtag agaatgcctt caagcggcat gcgacaagta agatccataa
    14101 tcgcgacgat ctttttcgca tacgaacgct gggctttcga ggggaagctt tgccaagtat
    14161 tgcctctgtt tctgaaatga ctctggaaac ggcgaatcag gaagaaaaac aaggcaccta
    14221 tctgtcgctg aaaggcggag aagttattga gcatcgacca gcaccactgc gtcaaggaac
    14281 gaagattacc gtcagcaatc tgtttttcaa tacgccagcc cgcttgaaat acgtgaaaac
    14341 actgcacaca gaattagcga acatcgggga catcgtcaat cgactggcat tgagtcatcc
    14401 atcgattgct tttcgtttag tacatgatgg aaacaaaatg ctggcaactg ctggaaatgg
    14461 cgatctgaaa caaacaattg cgggaatcta cggattatct accgcaaaaa aaatgctgaa
    14521 aatcgaagga aaagatctag attttgagtt atttggctat gtttcattgc ctgaggtgac
    14581 tcgtgccagt aggaattact tatcgacgat catcaatgga cgttttatca agaacttttc
    14641 cttgaataaa gcaatcgttg caggttatgg gtcaaaatta atggtcggtc gtttccctat
    14701 tgctgtattg gaaatcaaaa tggacccgct tttagtagat gtaaatgttc atccgacgaa
    14761 gcaggaagtc cggctgtcga aggaaaaaga actaacagag ctaattagta acgccattca
    14821 aaaagcattg tctcaagaaa atctgattcc aagtgcagcg gataatctac ggtttaagaa
    14881 aaaagtacct gatcagccta aaacagaaca aatcgaaatc gatttgtcct acgcttttga
    14941 agaagaaaaa cctaaaaagc caagtagttt gaattttgat cgagagacag gtaccttttt
    15001 cgtagaagaa ccaaagaaca catttgttga cagaattgtt gataagtctg tggaaaatgt
    15061 tgaaaactat tctggttttt ctgaagaaaa caccacttat ccagaaaaaa cagcgaaaac
    15121 ggtggataag tttggtgaaa atgttgataa ctttgtagaa aaaacagtgg aaaaatctga
    15181 aaagaaagaa tttcccgttg tggataacat ggagaaaaca aatgaagaac aagcagataa
    15241 atccgtagaa gaggatgaaa agatttacga ggaagaattg acactccatc ctgaatttga
    15301 tttcactcaa ggaaacgaca aaacattaga acaagccatc aagaaattag aaacggaaaa
    15361 accagtacaa cgttttcctc agttggaata ttttggacag atgcacggaa cgtatctttt
    15421 tgcccaaagt agcgatgggt tgtacatcat tgatcagcat gctgcccaag agcgcattaa
    15481 gtatgaatat ttccgggaaa aaattgggga agtatctgat gatctgcaag aattgcttgt
    15541 tccatttgtg ttagactatc caaacagtga tgctttgaaa ttaaaagagc aaaaacactt
    15601 gctagaagat gcagggattt atttagaaga atttggtcaa aacagcttta ttgtacgggc
    15661 ccatcctact tggtatccca atggagaaga agaatcgatt attcgggaga tgatcgatat
    15721 gctgctgaca actggaagtg tgagtgtaaa aaaattccgt gaagcaacag caatcatgat
    15781 gagttgcaaa cgatcgatta aagccaacca ttatttgaat gaagcacaag cacgtgtgct
    15841 gctgaaggat ctggcacgat gcgaaaatcc tttcaattgt ccccatggcc gtccagtatt
    15901 gattcacttt accaattcgg atatggaaag gatgttcaaa cgaatccaag acccgcatta
    15961 agtcaaagag aagagggaaa acatgaagat tgtattagca tcccaatcac caagaagaaa
    16021 agagttattg gctcatatcg tttcagaatt cgagattgaa cctgctgata tcgatgaaac
    16081 gccatttcca gacgaacagc ccgtcgatta tgttcgtaga atggctgaag aaaaagcacg
    16141 aattgtctgg gaacaagatg aaaaagaaga ccatttagtg attgccagtg acacgactgt
    16201 tgttctcaat caagagatca tgggtaaacc agaagatctg gctgaggctg aattcatgct
    16261 gaaaaaacta agcggggaaa cacatactgt atatacagct gttgttttaa aaacaaagga
    16321 acgagaagaa cggatactag cagaagcaca tgtcactttt tatcctttga cagacgaaga
    16381 gatcaaacgc tacttggaga cgggggatta tgcggataag gcaggggcat acggcatcca
    16441 aaatgctgcg agtgtattcg tcaaagaaat ttctggagat tattacagta tcgtaggatt
    16501 tccgattggt gctgtcaatc aggcactgaa aacattcatt taaacaaaaa agcggactat
    16561 ctgattttcg attgaaaatc catgatagcc cgtttttttt attgaaaaga ttacttatct
    16621 actctgacta a
//
LOCUS       DACEUZ010000054        16551 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.054, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000054 DACEUZ010000000
VERSION     DACEUZ010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16551
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.054"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(5..586)
                     /locus_tag="IXG44_001484"
     CDS             complement(5..586)
                     /locus_tag="IXG44_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1658210.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(769..1395)
                     /locus_tag="IXG44_001485"
     CDS             complement(769..1395)
                     /locus_tag="IXG44_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ1658211.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(1417..2748)
                     /locus_tag="IXG44_001486"
     CDS             complement(1417..2748)
                     /locus_tag="IXG44_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HAQ1658212.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(2903..3304)
                     /locus_tag="IXG44_001487"
     CDS             complement(2903..3304)
                     /locus_tag="IXG44_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAQ1658213.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(3390..3596)
                     /locus_tag="IXG44_001488"
     CDS             complement(3390..3596)
                     /locus_tag="IXG44_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1658214.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(3691..5193)
                     /locus_tag="IXG44_001489"
     CDS             complement(3691..5193)
                     /locus_tag="IXG44_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HAQ1658215.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(5211..5666)
                     /gene="argR"
                     /locus_tag="IXG44_001490"
     CDS             complement(5211..5666)
                     /gene="argR"
                     /locus_tag="IXG44_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAQ1658216.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(5764..6381)
                     /locus_tag="IXG44_001491"
     CDS             complement(5764..6381)
                     /locus_tag="IXG44_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1658217.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            6602..7975
                     /locus_tag="IXG44_001492"
     CDS             6602..7975
                     /locus_tag="IXG44_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ1658218.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            8050..9162
                     /locus_tag="IXG44_001493"
     CDS             8050..9162
                     /locus_tag="IXG44_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAQ1658219.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(9234..10871)
                     /locus_tag="IXG44_001494"
     CDS             complement(9234..10871)
                     /locus_tag="IXG44_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HAQ1658220.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAVVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(11039..11647)
                     /locus_tag="IXG44_001495"
     CDS             complement(11039..11647)
                     /locus_tag="IXG44_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ1658221.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
     gene            complement(12031..13182)
                     /gene="queG"
                     /locus_tag="IXG44_001496"
                     /pseudo
     CDS             complement(12031..13182)
                     /gene="queG"
                     /locus_tag="IXG44_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
     gene            complement(13201..13953)
                     /locus_tag="IXG44_001497"
     CDS             complement(13201..13953)
                     /locus_tag="IXG44_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1658222.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            complement(14094..15044)
                     /locus_tag="IXG44_001498"
     CDS             complement(14094..15044)
                     /locus_tag="IXG44_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAQ1658223.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            complement(15045..15884)
                     /locus_tag="IXG44_001499"
     CDS             complement(15045..15884)
                     /locus_tag="IXG44_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAQ1658224.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            complement(15881..>16551)
                     /locus_tag="IXG44_001500"
     CDS             complement(15881..>16551)
                     /locus_tag="IXG44_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAQ1658225.1"
                     /translation="TINPQKITGIIGPNGAGKSTFMKSLLELIPATTGKVTFSGQSVN
                     SVRKKIAYVEQRSELDLSFPIDVLGVVLLGTYPSLRIGQRPGKKEKERARQALKKVGM
                     EEYAKRQISELSGGQLQRVFIARALAQGAEWIFLDEPFVGIDALSERKIFDILQELKN
                     SGKTILIVHHDLHKVEEYFDEVILLNKQLIASGPVQEVFTSKNLQLAYGEIIRHLVKG
                     GEKK"
BASE COUNT         4467 a         3495 c         2907 g         5682 t
ORIGIN      
        1 attattaaag aaccttttgc gaagttaaca atctttcttt gatcaattga aaattctgcc
       61 taagtagtga tagatcctgt gtgactttca acaataagat acttccctgg atttcagcaa
      121 tcaacgcttt ggcttgacgg tctgcttctt catcggatag tttttcttca gaaagtgcca
      181 ggatattttg tttcgtatac ttgacccatt gattgactaa ttctagtagc aacagacggt
      241 attcttcatc cactgtactc aactcaataa tcaaattacc tacaggacag ccatgataag
      301 tgatctgtgt ctcatgatca gcgaaaatcc agtccaacat ttcttctaat gcttcttctg
      361 gctgttttcc agcatttaag atgtcttcga acaattctgt ctgccatacg tccatatgcg
      421 catggatcac cgctagacaa acttcttttt ttgaagaaaa gtaataataa aattgacctt
      481 ttcctgtatc agaagctgct agaatatctc ggattgacgt ttcatgatag ccttttttat
      541 aaataagatc tttggtcgct tccaagatct tttcctttgt tttcaatgat attctccttt
      601 ttcatttctc atagtcctat attatacagt aaattcatca ttttttcttc ttaatcgttt
      661 aaaaaagact gtgccaaatt atctgacaca gccctcacta taaaactgtt ctcatatagc
      721 tagatgttca ttgattcaag aactgcgagg acaaaagcaa ttcctatctt atgatagttg
      781 ctctaatact tcctctgttg tcatcacttg atctgcaata aagccaaagt tcgtcaacgc
      841 tgcatccatt gcttcttggc caggagcacc aattgcatct gtcgctacca caacattgaa
      901 tccactctct accaattcac gcatatggga atcgctacac aagtttccgt tcattccacc
      961 aagaattact gtgtcgattc cattttttct caattggagc gccaaatcat tgctttctgg
     1021 tccaaagatt ttatgaggag agctgatgat cgtattttca tccaaataag gtttcaattc
     1081 ttcgataaaa tcggctcctg aaccttcttc cactgattca tattgacttt ttctagcaaa
     1141 catctgctgc tcaagcatca tatgttctcc tgcaccgcca aattgccatt tatggtcatg
     1201 tgggtaaaaa taatgaggag aaataaagag tttgtatcct ttttcttttg cttgttttac
     1261 catttcaatc atattttcga tcgtattgta tttttctaag atatcttttg tcaaaccaaa
     1321 gccaccgcct gctggtttca agaactcgac ttgcgggtca gtgagaacta ctgctgtatt
     1381 tttattattg aacatggtgg attctccatt cgttatttat tttaatacat catttagagc
     1441 ttcactcatt gtcggatgag aatagatttg atctctcaat aacgtataag gaagttttcc
     1501 attcatagct agcgaaatca agttgatgac ttcatgagaa tcttccgcat agatacttgc
     1561 ccctagaatc aagtcagttt cagggtcgac taaaactttg aatacacctt taggatcttc
     1621 taatacttgt gctttaggga caccgcttgt catatacttg aataatttgt aattgacgcc
     1681 ttgtgctttg gcttgttttt ctgttaaacc tacattggat aaagtcggag tgataaatac
     1741 actgtaaggc acagctttcc ggttattcgt tgttcgttta ttctcgccct tcaactgatc
     1801 cataatgatt cgataatcat ctagagagat ataagtgaac tgcaatccgc ctttgacgtc
     1861 tccaatcgcc caaatgttgt ctgcatctgt acgaaggtat tcatccacag cgattgctcc
     1921 gcgatctgtt agtttaacat ctgtattttc taatccaagt gatgctgtac ttggttttct
     1981 tccagttgca gccaaaactt ttgacgcttg gattgttact tcttggccat cttctgtaaa
     2041 ggtgacggca gctgttgatt cttggtctgc tacttgatct acagatacac ctagatggaa
     2101 ttcgattccc gcatttgtca tgtcttctaa aatcatttgc gcgatatctt catcttcacg
     2161 tggaagaaat tcagaatggg catcaagaac gacaacttta gatccgtatt cattgaacat
     2221 tgatgcaaat tccaagccaa tatatccagc cccgatgatc actaattttt ccggtaattg
     2281 ttcctgatcc atcgcttgcg tagaatcaat caagtagcga cttgtattca atcctttgat
     2341 tggtaaaatg attggttcag aaccagtatt gatgaaaatc cgttcgcctt caacaatcac
     2401 tttttcacca ttattcgtca cttcgatttg atggttagaa agaaaacgtg ctgtaccgtc
     2461 catcactgtc ccagttgcct catcgctgat catatgataa tttttggcgc gaagcatgcc
     2521 cgtcaatttt tctttccgag caacagcctc tgtaaagtcg attcctcttt ctccattgaa
     2581 aatcaatgat ttagaaggaa tacagccgat gttgatacat gttccgccat acatacgtgt
     2641 tgacttttca acgactaaaa ccgattcacc ttttgaagct aacgccttcg ctaaagtctt
     2701 accgcccttt ccaaaaccga tcacaatatt ttgatatttt tccatcattt ttccctcgct
     2761 tcttttttac ctaccgattg gtacaaaaat atgataaccg ataattgtct attctccaaa
     2821 tcatctgctt tatttgtcaa aaaaaaccag acaaaaagcg gtcctttcac gtttttagtc
     2881 tggttcgatt tcctttattc ttctaaacaa tcgttacttc tacatcgata tttcctctag
     2941 ttgctttgga gtaagggcag aattcatgcg catcttctgc aattttttgg gcttcttctt
     3001 cgctgatgcc atcgatatgc acttctaata ctacaccgat ctgatagccg ccatcatcta
     3061 aattataaag agagacacgt gcttttactg tggactctgc tgtcattcct gcttctttga
     3121 tcatatgttg taccgcccca ttgaaacaag aagcataacc tgctgcaaat aattgttctg
     3181 gatttgttcc ttctgcatgg attcctggag gagtgatttt catatgcatc gagccatttg
     3241 gtgcctcgac ttctccattg cgcccgccat ggttgatcat tgctgtttcg tatgcttttt
     3301 tcattgtact gctcctttcg attgaccatt tgctacttgg ctataattat agtgtatgac
     3361 aagtaaggcg ctttctacaa aaattctgct tattcgaata ccagaatgcc gtcttctact
     3421 atttttccaa aaaacaaaaa aatactactc gtgaacaaca gcgtcatatt tgttccaaaa
     3481 taaatagcaa tcttttctac ataatctttt atatgattga ttttaagata catatatacc
     3541 agaaagttga ccccataaac caatactgtt aaaaaaataa ccatattcgt taccattttt
     3601 tcctcctttt gctggtctcc ctaccagcac tcgatttaac cataccactt tttttgccgt
     3661 ccaagcaact agcctttcaa aaagactggt ttattctgct gttaatgatt caagatcaga
     3721 aaaatgtccg aataattttg ctaattttac taacgccaac ttgttctttc cgaattcatc
     3781 attttctatc tgtaatacta gtaacggctc atgtaagctt aaacggatca aaaaccagcc
     3841 gtttccgtat ggctcttcta gatcatatcg aataccttct tcattttccg ggtcgaagtt
     3901 catccctttt tcttcttcca acagtcgctt catttcttct aatatcttgt ttccttgttc
     3961 gcgtggttca tcgctgaaca attttaatcg gatttcttgt acttctacgg gctgtttcaa
     4021 ctctttgatt tgttcatcta atgtgatacc ttttcttttt aattctggca aaagcatcag
     4081 gatctttgcg atgacatatg caccatcatc cagaaaatag ttttccttga atgcagcgtg
     4141 tccgcttgtt tcgatggcta gctgtgtatc tttcccaaga cggttaagtt ctaatgcttt
     4201 attgattaca tttcgatagc cggaaatata acgaacttgg atgcctcctt tttcttcaat
     4261 gaatctttgt agatgagaag aagtagggga attcgtcacg attcttgttc ctggatgctc
     4321 ttttaatacg attgtactta atactgcgat caaattattt cgattgatca ctgttccaga
     4381 agcactgaca actgcagaac gatccacatc tgtatcgaaa atgatcccaa gatctgcttc
     4441 atttgctaaa acagcctgct tgatactttc catcgcttct ttattatcag gatttgggat
     4501 atgattagga aatcggccat ctggttccaa aaattgggaa cctgttgaat ccgcacctaa
     4561 gctattcaat actttttcag caaagaatcc accagcacca tttccggcat caacaatgat
     4621 tttccaacca tttagtggtt tgctttctag tgttccaatc ccctttttaa tttttgtgac
     4681 taaatcttgc gcataaggtg tcaaaaggtc tgctttttct acgcttccag tttgttcgct
     4741 ttttattttt tgctgctgtg ataggataaa agcaatatct tctttttctg ctcctccttc
     4801 tgaggtaaag aattttattc cattataata atagggaaga tggctagcag tcagcataac
     4861 cgcagcatcg cacgagaact gttcaaattg agtggacata aacatagcag gcgtagtagc
     4921 taattcgaaa tcgatgacat gtatcccttg ttctttcaga ctttggatca acgcctcttt
     4981 cagtgctggt ccgcttatcc tactgtcatg accaatccca atcgtccatt ttttatttgg
     5041 atcggttgat ttcttctttt tctttatcca gtttgctagt ccccagccga tcttttttac
     5101 catgtctgtc gtcaagttta cttcatatcc ttcaactggt aatgcgattc ctcgaatatc
     5161 cgatccattt tgcagcgctt tcatatcttt catataaaag tcctcctcat ttattccctc
     5221 attttttcca gcttttctgc aaatcgctgt gcttcttctt ctgagcggca aataataatg
     5281 aatgtatcta caccggccat cgttccgaca acttcagggt aagccacttc atctagccaa
     5341 ttcgttacca catcggcacc atttcgttca gtcaatacga taaccatgaa ctgaacagac
     5401 tggatacgca aaacctcgcg tctaattgct gtacgcaaac gttcttcatt taaagaaaca
     5461 gatggctggt taaataatgt atagcgtact attttgtttt cattttgact ttttactaac
     5521 ttcatttctc gaatgtctcg agaaatcgtt gcttgagtca catcggcgcc tgcttcatgc
     5581 agccgcgcaa gcagctcttc ctgtgtccca ataggttgtt ctgtaatgat ctggcggatc
     5641 agtgcttgtc gctgtttctt tttcatatta gacacctctc aatgttgatt atagcaaaag
     5701 ataagtaata tttcttcata ttttggtatt ttttactttt attttttgtt ttcattcagg
     5761 ttgctaacct ttttctattt taacacgcaa aatcgtcttc attttttcag gtgctgattt
     5821 tcttggatcg ctgatgtaaa tctctttgtg ttctttagaa gctctttcat agccttcttt
     5881 tgcgatgaat gcttctaatt tctcaaacgt tgctggttct tcggcatagg gcccgacgtg
     5941 taagatttgg gcacaaagac cttcttccat ctttttgaat actacttgtt ctttcaagtc
     6001 ttccggaagt ttgctagaac gctgaatggc ttcttgtgct atcgcttctg taacaaagtc
     6061 aggctgtcgg atcatcaatt gataagaaaa atgttctttt ttcatcactg gttcatgcaa
     6121 atacttttct tgcagtcccc attgtccttc cagtggatag acagtataca tctgataatc
     6181 aggaatcagc caattttttt taggcgacat gcgtattgca tagctgactg catatagaca
     6241 ggctactctt cgtttaaagt cttcctgatt cgggtcgccg atccctctga tgctaaagaa
     6301 tgatttttta ggaacctcta gtatttgcgg gctttttttc atactatagc tcttgtcttt
     6361 acgccattca attttttcca tttttcttcc tccgcgaaat tccatttagt tatttattat
     6421 catacctctt tttgagtgac ttggctaatc gtgttgacac tatttttcag tttgtgctag
     6481 aataagtgaa gtaaataaat cgtacagata acacgtttcg gcgtgtcgag agagaggcgg
     6541 tttctatttt tataatagaa atcactctct ttttttacga aattttttag aggtgatgta
     6601 aatgacaaaa caaaaacagc tgcttttagt acctgcagta attgcaacag ggatgatgtc
     6661 ttttgcgggc gtattgatcg agacggccat gaatgtaact ttcccaactc ttatcagaca
     6721 gtttgattta acgactgcac aagtccaatg ggtaacaacg atttatcttt tgatgatttc
     6781 gatcattgtt ccgctgtcta cttatttgaa tcgcaatttt agtctcaaaa aactcttttt
     6841 agcttcaaat ctgcttttct tggcaggtat aatgatcgat ttcttctctc ctaccttcat
     6901 ggtcctattg tttggtcgat tgctccaagg tgcagcgaca ggtatcgctc ttccgttgat
     6961 gttccatatt atcttgacat ttgcccccct caatcgacga ggcgcgatga tgggtgtcgg
     7021 tactttgaca acggccattg ctccagcaat tggccccact tatggaggca ttatgacttc
     7081 acattttaca tggaatcata tcttcctgtt tcttgtgccc gttttgattc tttctttgat
     7141 tatcggctgg tttgctattc ccgagatggc agtacaaaaa agtggttatt tggattggat
     7201 aagcgtttta ggaatttgtc ttttgtttag tggaagtcta acatttttga gtacgttggg
     7261 aacagtttcc agctggattg ctttaatcat cggcggtatt ggctggttcc ttttttacta
     7321 tcgaagtaaa aaagtggagc atccactgat ccgactgtct attctttcaa ataaaacatt
     7381 ccgcttgttt ttgattggct tcttagttta tcagttttta ttacttggtg tatcttttgt
     7441 cttgccgaat ttccttcaga tcgttcaagg aagtagtcct tttgtcgctg gattagcgat
     7501 gcttcccggc gcagcggttg gtgctttgct ttctcctttt tccgggaaaa tgctggatca
     7561 atatggtccg aagaagccaa tttttgctgg gctgattctt tctttgattg gttggctagc
     7621 attattccta ctcataggac acgcttcagt gggtgtattg attgcttgtc atgtgtttta
     7681 tatgatcggt atcggattat cttacagcaa tatgatgacc accggaatga atgcactgaa
     7741 tgaagaacta caaggagacg ggaacgcaat gttcaatacc ttgcagcagt tttctggcgc
     7801 tgtggctact tcattagtag cggtcattat caattacgtc cagcatcata cttcccataa
     7861 ctatgaagtg tctactacat taggatcaaa agcagcatta ggcgtcttgt tgctgttgct
     7921 tctcgtaagc tttgctcgat tcgcctatta tctgttcttt gctaaaaaag cgtagattcc
     7981 atatacggca ggtgttgcat ctgccgtttt tttgtgtgaa aatggttgtt tgtaaaagga
     8041 ggctttttta tggaaaaaat tgccattatc ggtggcggga tcattggtat gaccctagcc
     8101 agttacttag atacatcaaa attcaatgtt gctttatttg atgaagggac aggacaggca
     8161 actaaagcta gcgctgggat tatctcccct tggttgtcga aacgcagaaa caaacaatgg
     8221 tatcgtctgg ctaaagacgg cgctgctttt ttccctaaac ttataaaaga tttccaactt
     8281 gatcaaacga tttacgcaca atcagggaca cttttgcttc gacctgaaaa agatttagct
     8341 gaattggctg ttcttgccga gaaacgaaaa gaagaagcgc cagaaattgg aaacatcgaa
     8401 tggctttcac ctgaacagac atctcaagca cttccgttat tgaaaccagc agtttcactg
     8461 cgtatcagtg gtggtggtcg tctggatgga aaagcatatt tagaacatct tggtcagcgt
     8521 ttagcacaaa aacaaatgga gcagatccag gcaaaagtca ctttgcataa gaagagcgat
     8581 tcttggctca tcaagggacc gtttggagaa tacacagctg atcgtgtaat tttgacacct
     8641 ggcccggcac ttaagccttt attacaagaa ttaggctatg ctgcagatat acgtccgcaa
     8701 aaagggcagc tcttagcatt tcagacatct tataagatga gtcaagactg gcctgttgct
     8761 atgcttgacg gagaagctga tttcattcct ttcatggatg gaaagatcct tttaggtgct
     8821 acccatgaaa acgatcaagg ctgggatttg gctcctacta gcgaagctta taagggattg
     8881 actaagagta cagaacagtt cttgactgat ccagatcaga taaaaaactg gccatacgag
     8941 ttccgtgtag gtacccgtgc ttatacgtca gatttttccc catttttcgg cgtactgcca
     9001 gatgatccaa gcttactggc tgctagcggt ctaggctctt ctggcttgac cacaggtcct
     9061 tatatcggct atctattagc caacttctta aatactggtc attggcctac ggaagattat
     9121 caaaaggaca tctccaccta tattacgaaa aatccatcat agaaaaacag ctttttcaag
     9181 taaggatccc gagcgatatt gcgcatggat tacttcttga aaaagctgtt attttagttc
     9241 aatttctgaa tcaaattctc caaactttct tttgcttcat ctggcaagag atcttttcct
     9301 atctgagctt caatgaatcg taaacgaata tcaagtcgtt gtttcaccag atttttatag
     9361 gacgtatcct caaaagaaac aggaaaatct ggtataggta aatagtggat gtcttcaaat
     9421 ggaccaaacg acttgaattc tgccgtttgt tgcacgattc tttcaacgat tgacaatgtc
     9481 gtttcaggtt tgattttttt atcatcgaaa tatccagtat tcaggacata gcaggctgtt
     9541 tgctgtttca aaaacaactc tcgaaaagct tgatagtctt ccctaagagg atagacacgg
     9601 aaaggattag caaatggttc aatgactagc tcatctagct gtctttttgt agtgatattt
     9661 tccgcgtctg atcgttttgt tgctaatgtt gcaccaaaag acgctgctaa aactgggtca
     9721 ttgatcttga taattggcgg aagcgaatca tctttcatga tccaaaaaac agcatcgatc
     9781 ttttcgctaa gatgatcgac tcggtcgggt gtcgcataac gtgatttgac tgtccgccca
     9841 tttccgtttc gtatatctcc tgttactagg actttttctc ctttacttgt acatgtcacg
     9901 cccacattct gacaagtaag gaaagaagaa atggccggat catcagacgg atagtcttgt
     9961 gttttatcaa agtaagaagg ttccaaggca gtagcaaaac cattttttcg agaaataacg
    10021 aaagcatcgt catgcaagat cgaaacagag tgaccttccg tatgttttgc taaggtaatc
    10081 gttgattttc cagaaccgga caacccaaat acagccatcg tataggtctg gtcattacta
    10141 gcatattgtt tcatcccacc gtggcaggca ataaagccat tgcgatttcc caatgcccaa
    10201 gcaagcgtca aagtcgcttt ttttaattct ccaaagtaac gtaatcctag cacaaccgct
    10261 gtattatgga gagcatcaaa caaaatgagt ccatttggaa atgtttcatg tgtccattcg
    10321 ggatcacaaa agatataaag atctccctca tcaaaggaaa cagacttttg ataagtctct
    10381 aagatcttcg aattcgctgg ctgaaaattc agcaaataag aatacaaatt gttctcaaat
    10441 ccttctggaa tcagcaaatg actttgcaca gaaaattctt cagataggcc aacgattact
    10501 tttgtatggt aaaactggcg cttgcgactt tgatacacag cttctcttaa aattttctcg
    10561 tatttgcttt gttccttgtc taagtcctca aagatccgcc gagcacttgc tgttcttccc
    10621 acaatgcttc catcattgaa tactagtgtc ctaacatcag ctggtaaatc tagttcttca
    10681 gcatgtgaga ttgttaaatc tgtgatgatc gttcctggac tcccttttgc tagttgataa
    10741 gctgtcttcc tatcaaaaac ttcatgaaca ttattaccgt aaaatgtcgt ttctacaatc
    10801 gatcgaatac tggataacag tggatttcca cttgtgattt cgtttaattc gagactctct
    10861 tttgtactca tggctcctcc tcattcccta aacaaaaaaa cgttttcttt attatacatt
    10921 attcgagttt ttatttgtac tgctgcgctg tgggaagcaa acgagttatt tttcacacaa
    10981 aaaaaccgag aacttgaata aacaagtttc tcggtttcat cattttcctt ttatcggctt
    11041 agtaccagcc gtttgctaac cagaatgttt tagcagcatc ccatgaacca taacgagatg
    11101 ttacatattg ttctgctaca cgttcttgat tggcagctga atagtcgccg tttaagtatg
    11161 aagcatctaa ttgataacgg ccgatgtaac gtccgttagt agctgtgtat gaaccgcctg
    11221 attctttttg tgcgatccat tcttttgcag agcttgaagt tggcgctgtt gcagcagctg
    11281 gggcagcagg agctacttca actgtttcaa caggtgcaac gtatgtatgt tcttgcgttg
    11341 ctgtttcagt tgtagatgct gtattttctg ttgttttgtt ttctgttgtt gctgaagaat
    11401 catttgaagt tgggattgtt aattgttcac caacataaat gcggttgatg tttgcgatcg
    11461 aatttttttc agcaattgca tcgatcattg agttgtctcc tgcaaatttt tgtgagatct
    11521 tagataatga atcacctgat tttactgtat aaacttcatc agcgtgagca tttgtagttc
    11581 ctacaaacat tgcagtagca ccagcagcaa aagttgtacc taaaacaatt ttcttaaatg
    11641 aattcatatt aaactccttt ttttctccgt tttttattct ttcgacgcta ttaactttat
    11701 catttagaaa tatttcatag gtaaaagaaa catgacattt aatgacaaaa taacgaatta
    11761 tttaatattt gtaacattta aacaaatatt tttttgatac aaaaccataa aagccttgtt
    11821 gtataagcaa agtaatcgtt tttctatgtt tttttatttt tgaatgaaaa acgcagattc
    11881 tgtaacattc actgcaataa tccataaaaa aaagcaaaaa aatttttgat ttttatcaaa
    11941 ttctcttaaa aaaatgtaaa aaatacaaaa aaaccagtaa ttgttacaac aattactgga
    12001 caaaaacttt gaataatgtt aactattttt ttattttaac tttctccgca ataatttctg
    12061 ggcatgttcc aattcgttta taacgacttc ttctgtttct ttagctaata tttcatctaa
    12121 tacatcaagc catttttacg gttcttttgt tcctagacgt ccaatagccc aagctgctgt
    12181 tcctctgatg acagggcgga catctgttaa acattctgaa atctcaggca atgccgttcg
    12241 atcacctaga ttagctaaag caatcaatgc gtttctctgc aaaggtttct tcccacgcca
    12301 tgaaccagct aaatgcccaa attgctgttt gaagtcttta ttagacatcg aaagcattgg
    12361 ctttaatttt ggatatattt cgtctacttt tggttccatc tcttcatgaa aatgaaaatc
    12421 ttttccttga ttgtaagggc agactaactg gcagatatca catccataaa tgacatttcg
    12481 cattttttta cgatattctt ccggcatcat cccttttgtc tgagtttgat aagacaaaca
    12541 tcgctgggca ttcattcttc cgtcaccaag caatgctttt gtcggacaag cagtcacgca
    12601 gcgcatacag tcaccgcaac caaattctcc tggtttatca ggctcaaata caatattagt
    12661 aacgatctca ccaagataga caaatgaacc gaactcttca gtgatcaaca atccgtttcg
    12721 tccaataaat cctaaaccag ctcgctgtgc tacagcgaca tcgatcagtt ctccagtatc
    12781 tacttgcgga gctagacgcc attcctcttc tttttgccat ttttctgctc ttgtttggat
    12841 gaattcgatc aatcggttca aacggtctct gagtatatca tggtaatcga tcccccaaga
    12901 agcacgagca aactgcccgc gtttttcatc cctaggcact tcttcatgga tcctagtagg
    12961 atacgcaagt gcaatggaaa tgatcgattt cggctgatca aatgtcattt ctggataaag
    13021 acgctcatcg atatttggat gttcaaagcc agaagtatgg ccagctgctt tttgttcttc
    13081 caacgattct tttaatgaat caaaaggttc cgccgtggtg aacccgattt tgtcaattcc
    13141 taatcgtttg ctttcttgta tgatttcttc ttttaatgtc atggtcttcc tcgcttgctg
    13201 ttaacggctg tataacccat aatatagttt ttcttttttc agctggacaa actggatctg
    13261 ctgatcggca atcttctttt cttccatcca tttttttctt gcagcaaata cttcccaata
    13321 atttgttgaa attaattttc ttttgttatc taatgcatta aaaaaattca atgaaactgg
    13381 ttttgtactt aacagttcat ggagccttac ctcttcccat ggcgaaaact cagacagatc
    13441 gcgggaatac aatacttctt catagagatt tttttgctgt gtgtacgtga atgtttgcgg
    13501 gattttccca ggcctagcaa tttctactag acgattataa aacatattca ttaatttgct
    13561 gttggcaata aattgacttt tataataggc cgactggatc tttttgatta ctccttctgc
    13621 tttgatgact gaaaataatg caggccatgc tttgacggtt atttcgtcgt tttgtttaac
    13681 taatacgcca ggtgtgaagc cgactggaga aaggaacaca aaataaccga taccggtatt
    13741 ttcatctaaa ggctgaaatt catcaaacaa ccatttgcct atgtacagct cttcatctgg
    13801 ttctgttgtc tcattttgat ttgctatgaa taatttagct aattctgctt caagaaattg
    13861 aaggtcttga tccttgtcgg tattttcctg ctggctgatc aatcgaaaga cttcttcttt
    13921 aatcgtatat ttatctttac gtgtaaaatt catagccaca ccttccttcc ttactctaac
    13981 tataacggaa tttcttcaaa aaaggtatct gtacgacgaa aaacagcata attttttttc
    14041 atatctctta taaaaaatgc aaggtctgct cctcaagcta gaccttgcac ttttcagctt
    14101 tcttttgttt gtgacaaacc ttcatggatc gtttcaatat tccatttcat catctgatag
    14161 taagaatctc cttgttctcc ttcttttgct atggaatcag tgaacagttt atcatagatt
    14221 ttcaaacccg tttcttttgc tacccgttcc atgcttcttg agtctacact ggtttccacg
    14281 aataaaaccg gtacttcttt tgctcttatc tgatcaatga tcgctttcat ttgatcaggc
    14341 gtaccttgac tttctgtatt gatctcccat atataagctg ctggcaaatc atatgctcgt
    14401 gaaaaatact tgaatgcccc ttcacttgtt actaataatt ttttgttctc ttcgatagaa
    14461 gcaaaacttt ccttcgcttg tgtatctaat tcttttagtt tttccacata tgcttctgca
    14521 ttttttttat agagtgtcgc attttctgca tctttttcag agaatatacg tgcgatttcc
    14581 tccacatatt ttattccgtt agataggtct agccaagcat ggggatctgc ttttgtatgt
    14641 tcttcaccgc tagttaaata aagaggttct acatttttgc taactgcaaa ataatctttt
    14701 ccttcttctt ttttagccgt ttccatcaag ttatcgaacc agctgttacc tgtttcaaga
    14761 ttcaaaccgt tgtataaaat aacatctgca tcgcttgcct ttttgatgtc ttctggtagt
    14821 acttcatatt catgcggatc tgttccgact ggtacgatac tgtggatatc tatttgatct
    14881 gttccgacct cttttgccat gtccgccaag atcgaattgg tagccacgac tgacaacttt
    14941 tctttacttc cttcatccgc ttggtcagaa gcctgctttc cgcaaccggc taataccaag
    15001 aaactaactg ccaagattaa aaagagtgat ttttttgttt tcatctatct gcttcctttc
    15061 tgaaataaaa ttccttttgt tggagaaaat aaaaaagcaa ggataaaaaa tgcggtacat
    15121 gctaacacga tcgtagctcc tgatggccaa ttgtaactat aactgaaaaa cacaccgaca
    15181 actgcactta gcataccgat tcctgcagct gtaatgatca ttttcagtaa atggttcgtt
    15241 agcaagtaag ccgtggcggc tggtgtgatc aacatggcaa tcaccaagat tgttcctact
    15301 gtctgtaaac tgactacagc cactaatgtc aaaaagaaca tcaaaagata atggatcagc
    15361 gatgtgttca gcccataagc ttttgccatt gtcggatcga atgatgtgat cttcaattct
    15421 ttataaaaga aaaagacaaa tatcaagacg attccactaa cgagtgctgt cagcaacaaa
    15481 tcgctttccc taacagctaa gacattccca aataaaatat gatacaaatc tgtcgcactc
    15541 tgggcatatg aaatcaagat aatccctaat gcaaaaaagg agctaaagac aattccaatc
    15601 gctgtatcat ttttcaatcg gctccgctga gtcacgaaac caatcactgc tgctgttagc
    15661 atcccaaaag cagtggctcc aaagatgtaa ctaaaaccaa acatataaga aatggctaca
    15721 cctggaagaa ctgcgtgaga gatagcgtct cccatcaaag acatccctcg taaaataata
    15781 aaagaaccga tgactccaga agcaagtcct acaagcatgg aagtgaccaa agcgtattgc
    15841 aggaattgat aattcattaa tccatcaata aaggattgga tcattttttc tctcctcctt
    15901 ttactaaatg tctgatgatc tcaccatagg ccaattgaag gttcttcgat gtaaagactt
    15961 cttgtactgg accggaagcg atcagctgtt tatttaaaag aataacctca tcgaaatatt
    16021 cttctacttt atgaagatca tgatggacga tcaaaatcgt ttttcctgaa ttcttcaatt
    16081 cctgcaagat gtcaaagatc tttcgttcac ttaacgcatc aatccctacg aatggttcat
    16141 ctaaaaagat ccattctgct ccttgggcta gagctcttgc aataaaaact ctctgaagct
    16201 gtccacccga tagttcgctg atctgtcttt tggcatattc ttccatccct acttttttca
    16261 aagcttgtct tgcacgttct ttttcttttt tcccaggtct ttgtccaatt cgtaaagatg
    16321 gatatgttcc taaaagtaca acgcctaata catcaattgg aaaggaaaga tccaattcac
    16381 ttcgctgttc cacgtaggct atttttttac ggactgagtt taccgactgt ccagaaaagg
    16441 tgactttacc agtagtagct ggtatcaact ctagcaaaga tttcataaat gttgattttc
    16501 ctgcaccgtt tggtccgata ataccggtaa ttttttgtgg atttatcgtc a
//
LOCUS       DACEUZ010000055        16159 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.055, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000055 DACEUZ010000000
VERSION     DACEUZ010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16159
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.055"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            <1..374
                     /locus_tag="IXG44_001501"
     CDS             <1..374
                     /locus_tag="IXG44_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAQ1658226.1"
                     /translation="LKEQDLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVR
                     VPTKRWQNDVNAMLEQMTKKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHL
                     VSEALLGGSSSTNSSETNVTE"
     gene            complement(449..733)
                     /locus_tag="IXG44_001502"
     CDS             complement(449..733)
                     /locus_tag="IXG44_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAQ1658227.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(723..1457)
                     /locus_tag="IXG44_001503"
     CDS             complement(723..1457)
                     /locus_tag="IXG44_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HAQ1658228.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            1586..2209
                     /locus_tag="IXG44_001504"
     CDS             1586..2209
                     /locus_tag="IXG44_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HAQ1658229.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            2401..3522
                     /locus_tag="IXG44_001505"
     CDS             2401..3522
                     /locus_tag="IXG44_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HAQ1658230.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            3529..6657
                     /locus_tag="IXG44_001506"
     CDS             3529..6657
                     /locus_tag="IXG44_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HAQ1658231.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            6676..9870
                     /locus_tag="IXG44_001507"
     CDS             6676..9870
                     /locus_tag="IXG44_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAQ1658232.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(10016..10723)
                     /locus_tag="IXG44_001508"
     CDS             complement(10016..10723)
                     /locus_tag="IXG44_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAQ1658233.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
     gene            11488..11886
                     /gene="spxA"
                     /locus_tag="IXG44_001509"
     CDS             11488..11886
                     /gene="spxA"
                     /locus_tag="IXG44_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HAQ1658234.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
     gene            12218..12871
                     /locus_tag="IXG44_001510"
     CDS             12218..12871
                     /locus_tag="IXG44_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HAQ1658235.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            13097..14095
                     /locus_tag="IXG44_001511"
     CDS             13097..14095
                     /locus_tag="IXG44_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HAQ1658236.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            14271..16079
                     /gene="pepF"
                     /locus_tag="IXG44_001512"
     CDS             14271..16079
                     /gene="pepF"
                     /locus_tag="IXG44_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HAQ1658237.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
BASE COUNT         5617 a         2708 c         3345 g         4489 t
ORIGIN      
        1 aattaaagga acaagatttg ttgaaagaca ctgttctgat cggactagga acgaatggct
       61 cattctcgga agctcagttt gatagtgtga tggatgcaat cggtgatcga aaagtatact
      121 gggtcaatgt ccgagtaccg acgaagcgct ggcaaaatga cgtgaatgcg atgcttgaac
      181 agatgacgaa aaaatacgac aatatgacgc tgattgattg gtacagctac agtaatgacc
      241 atagtgactg gttttatgat gatcaagtcc atccaaatcc tgaagggatg acacattaca
      301 ttcacttagt cagcgaagcc ttgctaggag gaagttcaag tacgaacagc agtgagacaa
      361 acgtgacaga atgatagcta caaaaaactt caggcaaaaa catttgtttt tgcctgaagt
      421 ttttttatat ttaccgtgtt tttaacattc atatagattc aggaagcaaa acggttgagt
      481 gtgtctgtaa ataacgttct ttttgttttc gggtgagttt tttgaatgca gcttctgctt
      541 ttgttgcttc gcttcttgaa gaaaatactt ctgcatggat catcttaaca ggtcttcttt
      601 tttgaagacg agtatattta gcaccagtac cattgttatg ttcctctaat cgtctagaaa
      661 gatcggttgt atatccacca taaaatgtct gatctttaca gaataatacg taaaaataat
      721 gttcatttgc catagagcat ctcacgaatc tctgcagtgt attctccatc catattgtaa
      781 gtgatcaatg gtggagcgac tttgaatccg tctaatttgc cttctttgat tccttcaact
      841 aacaaaatat ttgcctcttt tccaggtttt gggtaaacaa attggatcct tttaggtgca
      901 attcgatagt cttccatgac ctgaatgatt tctaagaacc gatcaggacg atggaccata
      961 gtaaagcgtc catttgtttt tagtagctta cttgaaatat cgatcacttc ttctaacgtc
     1021 gtatgaatct catgacgggc gattgccagg tgtttattag ggttcgtttt atttgttggc
     1081 agccctttga aataaggtgg attacagacg actaagtcac aagaattatg tcgaacgaca
     1141 gataagctgt ctttcagatc gatggtgtgg acagtcattt gttcatttaa atggttaagc
     1201 gcgatactac gtcttgccat gtctgctaga cgaggttgaa gttcgatagc gtcgatagcc
     1261 gcctgggttc gtttacttaa aaaaagtcca actgcgccat ttcctgcaca aaggtcaaca
     1321 atcttccctt ttttaggtac acgggaaaag tgagcaagca gcacagcatc taaagaaaaa
     1381 gaaaacactt tggaactttg gataatcttg atatcttctg cgtataattg gtcgattcgt
     1441 tcgtcttcgt gaagcatgga ttccctccaa aatgaaaaaa atagtcgtct gtattatact
     1501 atttttatta aaaagaacca aggtggcttg tcaaacctct attttttatg catactatga
     1561 ccataagaag ggaaaggaag agactatgtt ttatcgattt atgcgcgggg tagttcgagt
     1621 actattagcc ttagttaatg gaaatgcccg ctatgagaat aaagaagttt taccgcaaga
     1681 tgagaattac atactagttg ctccccatcg tacttggtgg gacccattat atctggcggt
     1741 agctgcagca cccaaggaat tcagctttat ggcaaaagaa gaactgttca aaaatccagt
     1801 tctgcgattt atattaactc gatccaatgc ttttccagtt aaaagagaaa atccaggacc
     1861 aagcgtcatt aaaacacctg taaagatttt gaaatcgaca aatttgagtt tgatcatgtt
     1921 tcccagtggg acaagacatg ctacagaact taaaggcgga atggcattga tttccagaat
     1981 gtctaaagcg aaaatcgtgc cagctgtata tcaaggtccg ttgacacttg gggatctgtt
     2041 caagcgtcag agagtgacag tgcgtttcgg cgaaccaatc gacttatcag atatcaagaa
     2101 aacggataaa gaaggtatgg cggagatcga acgacgaaca caagcagctt ttgacaaatt
     2161 ggacaaagaa gtgaaccctg atttcaaata tgaagtcaaa aaaaaataaa atgataagtt
     2221 tatgcgtggg cggtcttttg aatctactgt cctgttcagt tcagactttt tcaatgaaaa
     2281 cgagggagag acgtcaagtc tgccctcttt ttgcgttcca atatgtaaaa tagaagacag
     2341 gtgtcatgta atggatcaaa gtgttataat aattagaagg acattttttg aggtgattga
     2401 atgcgttttt tacatacagc cgattggcat atcggaaaaa aactacaagg ttatgacttg
     2461 attgaagacc agcgtcatgt actatcttct atactagata tcgcgaaaga agaaaaagta
     2521 gaggcaatcg tgattgccgg agacttatat gataggagtg tcccagctgt tgatgccgta
     2581 gaattataca atgaattgat ggtcgattgg aatttaaagg aaaagatccc gatttttgct
     2641 atatcaggaa atcacgatag cagtacgaga ctttcagcgg gtacgccatg gttcagttat
     2701 tcgaactatt ttttgcgaac acagctagca gaagcatttg aaccagtcga gtttaaaaac
     2761 gtacaatttt tctttcttcc ttactttgaa ccaattgcag cgagattata ttttgaagat
     2821 gacaatatcc gaacgatcaa gcaagcggta gccaaggtga tagataaaat gcaggagcta
     2881 tttgattcag aaaaaaaaca agtattgatt agccattttt ttgtaatggg aagtgtaaaa
     2941 tctgattcgg aaacgaaaat cgaagtaggc gggttggatt cgatccctgg tgaactactt
     3001 acaccgtttg attatgtagc actaggacat ttgcataatc aagctgcttt gagacaggat
     3061 aatgctcgct atagcggttc cccattgaaa ttctctttat ctgagatcaa ccaaaaaaaa
     3121 ggcgtatgga tcgttgacct gacaacaggc ctggagttga attttaggga gatcgaaccc
     3181 ctgcatgata ttgaagaaat aacagcgagt ttcaaagaat tgctggatcc tgccttttat
     3241 cagtccgtca atcgggagaa ttacatctat atctatctca ctgatagaac gatcatcccg
     3301 aatatgatga accagcttcg gcaaatatat cctcgtattt tggggattga acgggcaaac
     3361 ggtcgagcag aacaaacaaa gcgcaaacga aaagtactag aaatgaaaga tcctcaaaaa
     3421 ttagcagaag cattttttag tgaaatgacg gaagagcagc taactgcaaa ccaagaaaaa
     3481 tggctcggag aaacgttgca agaaatcatg gaaatggaat gatcaagaat gaaaccgaaa
     3541 aaattacaac ttaaaaattt tggtcctttt atcaatgaga ccgtagattt ttcacgtttg
     3601 acagaagcac cgcttttttt gatcagcggg aagacaggag ctggcaaaac gacgattttt
     3661 gatgggatca ccttcgcact ttttggtgaa acttccggaa gacttcgctc tggaaaagag
     3721 atgcgttctt tgtttgctac accagacgaa gaaacaagcg tgactttttc ttttgaacat
     3781 cagcagatga gctatgaaat cgaacgaaaa ccagaacaag ttttagcaaa acgaaaagga
     3841 aacggaacaa aaaaacaggc agccaaagta acgttgacca tttttgatgg gaaaggaaaa
     3901 gaaataaaac aaatcacaaa acgtacagaa gcagatcagt tgatcagaga actgatgaat
     3961 ctcgatgcga aacagttttc tcaaatcgtt ttactacccc aaggagaatt ccgtaatttt
     4021 ttggtttcat caagcagtga taaagagact gttttacgtc atttattcgg tactcaattt
     4081 ttccaacagt tcaatgagcg attaaaagaa aaagcaaaac aacagcaaca aaaattggat
     4141 catttagagc aagagctgca tcttctgcaa aaaaggtttg ttttagtcaa agaagaggaa
     4201 ccagcagcag ggtttgaagc cgttttagcc caatggacaa accatcaggc aatactcgaa
     4261 aaacaaatca cggaagaaaa agaagctcta acggaacttc aggaaaaaca aaagcaacta
     4321 gaaaattcct actatcgttt tgaaagcttg aaaaaaagtt atgaagaaaa aacgatatta
     4381 ataaacaagc aaaaggaatt agctgctcaa aaagacgaaa tagaggaaaa gaaacgatgg
     4441 attgggtatt atgaatttac gcaacgatta gtcgaaccaa tccagcgcct acaagaaata
     4501 aaagttgaaa aaatagaact gtcacaaaaa agtagtgagc aaacaaagaa actagcgatg
     4561 actacgcaga attatgaaga gtggcaaaaa aatgaagcaa accgcgtacg ccgacaaaca
     4621 aaaatcaaag aagctgctca aaaactgcaa aacaaacaga tgatggtgcc aattgtcgaa
     4681 tccttacgta gtaaaaaaca agacgcagaa caattaacaa tttcattgga taaagaagaa
     4741 ttgaatgccg agaagctgta tcaaaatctc gctatctatc aggaaagaac tcgaattctt
     4801 caagaaaatt tactggatca ggaaaaactg caagaagatc gtcttgcttt tggacagtta
     4861 aaatatctag atgaaaagca gcaagaaata gaaaaacaaa aagcaaaaga cactgggtcg
     4921 ctcgcacaga taagagaaga actcaaaaaa gcaactgaag aaataacaaa aaatagtcag
     4981 ctattgaaag aacagacaga acaagcagaa cagctgaaaa gccaatgggc aaagcagcaa
     5041 attgctcgtc ttcagctttt tttgcgtcca ggggagccat gtcttgtttg tggatcaacg
     5101 gaacacccca tacaacaatt aaaacacgaa cagccaaata aggaagatat cttaaaagta
     5161 gaacaacaac tggcagaagc tgagtctgct gtagaaagaa ctcagcaaat cattgccaaa
     5221 agtgaagcac atgaaaataa tcttcaacaa caattatcag atttagaaca aaaaatcatg
     5281 gcaacgaata tacaattggc tgaacaagaa gaacacttgc aacaggcttt gaaaaaggca
     5341 tttcctgaga taacagaaca aaaaacaaaa acaactcttg tagtaatcga acaatcttta
     5401 gatagacgaa aaagcaagat ggccaatgca agaatagagc tggaagaaat aagacaacaa
     5461 gcagaaaagc tcgaacaaca attagctgtc tacaaacagc agcagacaga aaaaaataat
     5521 cagctccagc aactgcaagg agaaatcaaa agtttagaac aacaattgca agatacggat
     5581 agtaactgtt tagaagagga gatacgacga cttgaaaaac agttagaaga agactcgaat
     5641 caactagctg ttgagcagaa agcaggagtg caattgttgc aagatcttca tacgcttcgg
     5701 acacagcttg atcttcaaca tgaacaaata gaaaaaatag atgaaaaaat agaaaaggaa
     5761 caagaaagaa tagctaaaaa gttagaaagc cagtcgtatt tttcaacaat tgaagaaatc
     5821 tgtgcatttg cacaacagca agcacaatat aaagaaaacc aacgtacagt tacttcttat
     5881 catgaagagc aattagtgac agctgaccgg ctgcgtcaat tagaaaatat ccagctaact
     5941 gagacattcc cagatacaga agagatcagc caacagttag aagaaataag acgtatgatc
     6001 gaaataaagc aagaaaagat atacggttta caagaacaaa aaattagtaa tcaaaaaatc
     6061 tacgatgaat gtctttcgat ttatcacaca agccagacga aattagatga gcttacgcaa
     6121 ttacagcagc tttcgcaaac attcaacgga gaaaatccta agaagactag tttggaacgg
     6181 tatgtgcttc aggtttatct tcaagaagtg ttacaagtag caaatgatca tctccaacgt
     6241 ttaacgaaaa atcgttatca atttgagcta gcggatacca ttggcagtta tcgagggaaa
     6301 accggattag aaatcaatat ctatgatgat caggcaggta tgtctcgaag tgcgcatacc
     6361 ctgtctggcg gtgagagttt tattgcagca ctttctttag cattagctct tgccgatgtg
     6421 atccagacac aagctggtgg tgtagcaatc gatgcattgt ttattgatga aggatttggg
     6481 tcattagatg aagaagcttt ggaaatggca atggaagctt tagaaacgat cgaaagtgaa
     6541 ggaagaatga tcggcatcat tagtcatgta agagaattga aagaacgagt attacaacaa
     6601 ataagaattg atacagaagg tagcggacaa agcaaaatac gctatcaact tggataaaaa
     6661 gggagattgg taactatgaa atggagtatt ccagaaaaag tcattgcacg tggacgtcaa
     6721 tacttgcaag aaggccgagt gttatcggtt gtccctgacc aagaagaaaa cgtgtggcat
     6781 gcagaagtct tgggcagtga attgtatatg gttgatttgg atggtacagc aaaagagata
     6841 gattactgcc aatgtcccta ttgggaggaa cataaatatt gtaagcatac agttgctgtg
     6901 gaactttatt tacggtcgaa aaatctatct cgtatcatga aaaaagacca gccaatgcta
     6961 gaaccggtaa aagcaacaag tgagggagaa atcattgcaa aaggtttttc tcgtctaaaa
     7021 acacaaaaca atcctcaagc ggtgcaacct ttgattattg aataccaagt agaaacgatt
     7081 gagactaatc agtatcaccc agagctttct attttggcac tttacttgcg tatcggttca
     7141 ttggcatcac cgaaaaaaac atatatcgta aaaaacatct atgatttttt acaagcttac
     7201 acagaaaaag aaacttacac tgttaataaa cagtaccagt ttcgtctaga taaacgggcg
     7261 tttcaaaaag aagatcaaaa aatactcacg catcttgcag gaagtgctca gacgcatcaa
     7321 ttattaggta cgaacggctt acaagtcaaa ggaaaactag ataaaagata tctgcttctc
     7381 ccagtagaac aagcccgctt cctgttagag cagatgaatc agactacacg tttgcatctg
     7441 cagataaatg aacaaaacga tcaaggaata caattttccg atcaaaacaa acctttatca
     7501 ttcaaagtgg aaaaatttgg agaagattat ttgctgcaag cattgaatga ttttgatttt
     7561 ttctttatgc attatcagtg gggagttatc caagggacat tttattctct gacgaaatat
     7621 caacaaacgg tttatcaaac ttggaaacaa ttgattcgcc gattggagaa accagaagta
     7681 gtgttcaaga aaaaagaatt accgtctttg tttaaagaga ttatccctct gctgtcagag
     7741 atcggcgacg tagctattgc ccctgcagta gaagaggaag tagcagatat ccccgtcgaa
     7801 tttattttct ttttccgaaa agcaaaagga aaaatccaag ctcgagtcga tttcgtctat
     7861 ggcgaagtca tctattcgac tgacccgaag catgaggtct cgacagaaca ggcttttcag
     7921 gtgttacgtg acacagtaga agagaaacga gtacttgatc tattcagaat gtatcgctac
     7981 cagaaaaatg aaactggtta tgaacgtcca ttgccaagcg gggaagaact atatgccttt
     8041 ttccgcacag aactatctgt cttccgtcag tttggtgaag tcagacttgg aagaaaattg
     8101 cgagagttgt atttagatgc gcataaacat caaccgaaaa tcgaagtaac cgagaccggt
     8161 tcatggctcg atattcgatt cgatgtgaca gggatagagg agcaagaaat cgatcatgtc
     8221 ctgcaaagct tgctgagaaa tgatgctttt tatacattag aaaatggtca ggttctttct
     8281 tttgattcag aagaatttca acaaacaagc caagtgttgc aacaattacg ggaatcgata
     8341 cgtacagaag aaggaacgat ccatgtacct aaaaatcaag gattgatcat tcaaaatcaa
     8401 ttggaaaaaa gcaatgctac ttttagtgaa tcgttccaaa cgatggtaca agatctcatt
     8461 catccagaaa gataccaggc acagcttccc aaaggattga atgcaacgat gagagattat
     8521 caaaagcaag gttttcgatg gttgaagatg ttaggtcatt atcagtttgg cggtatttta
     8581 gccgatgaaa tgggacttgg gaaaacacta cagacaattg cctttttact ttcggaaaaa
     8641 gaagaacgga aaagcttctc tgctttgatc gtagcgcctg cgagcttaat ttacaactgg
     8701 caggcagagg tgagaaaatt cgccccttct ttaagtatcc aagtaatcaa tgggaataaa
     8761 aaagaacgtg aagaattact ggctaaagac acagatatcc gagtgacttc ttatgccagc
     8821 ttaagacagg atctagctaa ttatcagtcg caaaaaattg attatttgat tcttgatgag
     8881 gcacaaatgg tcaaaaatag cagtacgaaa actgcacaag cattgagaga attagctgta
     8941 cctcagcggt ttgcactgag tgggacacca atcgagaata atttagaaga attgtggtca
     9001 ttgtttgcaa cgattatgcc aggatttttc ccgactaaaa caaaattcag ggaacttgcc
     9061 gcagaagaaa ttgcgcaaat gattcgaccg tttattttac ggcgagataa acaaactgtg
     9121 ttgaaagact tgccggaaaa aacagagatg aatctatatt ctgcacttac agaagaacaa
     9181 aaaactgttt atttagctta cttgcgtcaa atgcgtgaag aaatcacatc aatggattct
     9241 gaagcattca agaaaaaccg gatcgggata cttgctggtc tgacaagact gcgacaaatt
     9301 tgctgcgatc ctcgtctgtt tattgaagat taccaaggcg gttccggcaa gctcgaacaa
     9361 gtaaaagatc tactggtagc tgccaaagag aacaaacgaa gagttttgct gttttctcaa
     9421 tttacaggga tgctgactat cttgcaagaa gagttagccg agctagggat ctcgacattt
     9481 tatttaagag gcagtacgaa accacaagat cggttaagta tggtagacgc atttaatgct
     9541 ggcgaaaaag atgtttttct gatttcctta aaggctgggg gaactggatt gaatctcacg
     9601 ggagcagata cggtcatctt gtatgacctt tggtggaatc ctgccattga agaacaagct
     9661 gctggacgtg cacatcggat cggacaaaaa aatgtggtag aagtgtggcg tatgatctct
     9721 gaaggaacga ttgaggaacg catggattca ctccagcaag aaaaacgcga actgttccaa
     9781 aaagtgatcc aaggtaatga agaacagttg acgaaactga ctgaagaaga catccgcatg
     9841 attttaagtg taggagaaat cgaggagtaa gagactcgaa aaagcgttca gtaatgggta
     9901 agaaagcctt tctaaagaag cgattgttta tttgatttaa aaaatagcaa ggaggctggg
     9961 attccatttt atcccagctt ccttgctatc atttttttat ttctattttt aaaggttata
    10021 caaagattgt cttcattgtc tctaacagct gatcaaaaga agaaatctcg atatcgcaaa
    10081 ttggagtctg ttcaattttg cgttctcgat ggttgaacca aagcgcttgc cagttagcac
    10141 tttttgcacc tagaacatca ttgtcgtaat tatcgccaac gtataaggtc tcttctggaa
    10201 gcatatgaaa ttctttctca gctagctgaa aaatctcttt ttctggtttt tggaaacctg
    10261 ttgcttgaga aatgagcata ttgcgggaag gaatccaatt gttcaattga agttgcttca
    10321 gtttttttgt ctgatggtca gtcggcccat ttgtgataat tcctaatggg acattctttt
    10381 ccttcaagaa atcgagggtt tttttgacct cctcatgcaa gcagatatta tcaagttctt
    10441 cctcgtaaat tttttgaaat aagaggccat cttcttccgt aatatgaggg tagtctaaat
    10501 cttttagtga ttggctgatc cgatgtgctc gcatatattc tagtgtccaa tctcctgcca
    10561 ttacttttgg gaagttttca tcactatgat ggcggaaccg gatatatagt ggatgcatat
    10621 ctgcatctga aacaagcgga acgactcttt tgaccgcgtt tcgaaatggc tgctgctgat
    10681 catacatcgt atcatcaaca tcaaacacaa ctgatttaat caaaatttta cacgcctttc
    10741 ggttttcatt gattttttca caaatccttg actattgtaa cagaaaaaat tgtctaaaaa
    10801 agctgataaa taaaggtttt tcttactata tttctatttc cttgacagaa taaggttttt
    10861 atatttcagt ttttttcact aaaaatcgtg aaacacgtca ataagtttga tgaacaaatt
    10921 aatcattatg gtagaagtcg attgatttat tttaatgcaa tctctgtgga attttcgaaa
    10981 gatttatttt tttaatacag tctcaatata actattcaaa cataaaataa cgttatttga
    11041 tttatttgtt tatagtaaat tttatttagt aatcattgct ttaaaatact acgcttatat
    11101 aacagagaaa ataagcgtta taataaagaa cagtattatt taaaatgaca gaaaacatta
    11161 ctattaaaga aataaaaaac ataaaaaatc ttaagaaatc ggtttcattt agcttttgag
    11221 ataaaataat agtacagttt tatacgctgt ttcatatgtt tagaaaattt gaattagatt
    11281 tgaaaactct aatttttaaa tgataaaaaa agcgagaatg ctggttttac tgtatattaa
    11341 ccttagataa ctttcatttt tttaaaaaaa ttatgaaaaa aaggtagcta tttaaaagtt
    11401 gattatgtat aatagaaaca gatgggagat tattatgatc ttctgttaca aaaaagtgat
    11461 tcttatcacg aaaaggagtg gtgcacgttg ttaacattat atacttcccc aagttgtact
    11521 tcctgcagaa aggctcgtgc gtggttgcaa gaacatcaga ttccatttgt tgaacgtaat
    11581 attttttcag aaccattgaa cagttctgaa ttaaaagcga ttttacaaat gacagaagac
    11641 ggaacagaag aaatcatctc gacacgttcc aaagtattcc aaaaactgaa catggattta
    11701 gatgatctgc cgttacaaga attattggaa cttgtgcaaa acaatcctgg attgcttcgc
    11761 cgtccaatca tgattgatga taaacgtctg caagttggtt ttaatgaaga tgaaattcgt
    11821 cggttcctac cgcgcgatgt acgacaatta gaattacgtc aagcacaatt gatggctgga
    11881 ttatagaagg ataatacgaa acttggaaaa gactattaca aagatagttt ttccaagttt
    11941 ttttgttttt acaaaaagta ttcttgatta gatgtataaa aaggtagtag aagatgatga
    12001 ttgcacgaaa aatagagagg gaaaattagc tgaaaagtcg gctatatagt attcgtctgc
    12061 caaaaatgtt atagttaaat aagatagctt gaaaggttga aaaatccgct ggttttatta
    12121 aattcgctta acagcgagat tttttttctt tacatttaag ctgatttcgc tacaatgaat
    12181 ctatattaaa cattgtggca aagtgaggtg tagcaaaatg gaaatggaac atattaatga
    12241 aaatacgatc cgtgtactaa ttggtcatga agatttagag gaacggggcg tttcattttt
    12301 ggatttactt ggcaatcaca aagaaatcga aaatttcttt tatagtattt tggaagaagt
    12361 tgatatcgaa gaggagttcc gaggaagcga agctgtcact ttccaagttt tgccaaaagg
    12421 agacggatta gaactgttta tcagtaaaaa tcttccggat gaagaaattt ctcaatcaga
    12481 agattctact gttgattcag aagatgtgag tgactttctt cgtcagcaga ttattggcag
    12541 cgatccggag gaagaattat ctggaagcgc cctagaagaa caggaaagac aagcaacttt
    12601 tgcttttgaa gattttgaac aagtgatcca acttgcaaca gatgtcgaac tagagtctgc
    12661 ttggactgat ttgtatttat tcgaagagcg ctattaccta gtcgttcact tctggatgga
    12721 aaatctcaac caagcggatg tagagaatca gattgctcga attttggagt ttgctgaaaa
    12781 gagcgatcgt acaccagaaa cattgaatga atatggacaa tgtctgatgg cgcgtaatgc
    12841 tatcgagcga acgaaatttt attttgtata aatcagcatt atagtaagag actgtgacaa
    12901 aaattgtgtc gcagtctttt tttcgtataa atgtgttcaa aagcttgcat ctgcggtaac
    12961 aagcccaacc aaacgaaaaa tattgggaac tatttttgtt tagctgttct tattacttga
    13021 tgcagaatag ctttttcacg ccctcttttt tcgtgtaaat aaattcttag acgtaaataa
    13081 gttcagaggg agaaaaatgc tgcttgcaat gaatgaatat aataagtgct gttttgctaa
    13141 acatgcagaa aaaggaaaga aatattattg tcgtggctgt cgaaaaagag tgattttgaa
    13201 aaagggaaag aaaaaatgtg ctcattttgc ccatcaaaaa tcagataact gttcggtctt
    13261 tagtgaaaga gaatcaaaag agcacttaca gttaaaggaa tgcttcatgg attggctggg
    13321 acagtcagta gaaccagtat ttttggaagc ctatttgccg aggttgcggc aaaggccgga
    13381 tattttgttg gcgaatctag caatagaaat ccaatgttct agactgtctc accagagatt
    13441 tattgaaaga acccaaaatt atttgaataa cagttaccaa gtatggtgga tacttggtaa
    13501 ttcttttctt ggacaatcgc aattctcatt gatagaaaaa agttgctgct actataatag
    13561 aaaaagagga gtgcattgct ggaaagctga tctcaagaaa caaaagcttt atttgtatca
    13621 tcatattaca gagactgtat cagggcatat ttccttttgc agtagctgct ggactttttc
    13681 tagtagggac cttaaagaaa tattcactag taacgaaatt aaaatgaacc aaatgaaaaa
    13741 aatagaaaaa gtgagtgaag atgggaagaa atggctgaca cgccaactta ttcacaagca
    13801 aaagaataca gtatccatac aagaacaatg ctacttgcgt cataaacatc ttttgcatct
    13861 atcatcgtgg atctatcaaa aaagtcggtt tttcttttat ttacaagaac aggtttttct
    13921 ttatcgaatg ttgtacgaag aagccttgga aaaccagaaa gttcctgatt tcaactcatg
    13981 gctttgccaa ataaaagaac ataaaaggga atggcttttt cctatgattg atgaagagat
    14041 ggtctatcag cagtttttta acgaatgcat acacttgtct tctttgaaaa aatagtaagt
    14101 ataaaaatag gctaggaatc ggttttctat gtaaaacaag atttagttat gctaaaattg
    14161 atggaagcag acacatgaat gagtagaaaa gttagatgct gtgacatgtg gatgttagac
    14221 tatgatctat tttgatagat agagctaagg gaagaaaagg agtggatttc atggaagtaa
    14281 agcagttgcc aaaacgagaa gaattgcctg aaaatttaac ttgggacttg accaagatct
    14341 tttcaagcga ccaagagttt gatgagaaat atttggaatt atcagaagag ttaaaacaat
    14401 ctgaaaaaca caaaggaaca cttgatcaag gcgcttctca atttttaaat gcgattgaat
    14461 tcgtattgag ggtttatcgc caaactgaag tcatttatgt atatgcgcac cttaaaaacg
    14521 atcaagacac tggaaataca gattaccaag cgctttatgc aagagcaagc agtctgtttt
    14581 cgaaagttag tgaagccgtt tcctggtttg aaccagaaat attgcaattg tcagatgacc
    14641 agatttggca atatttcaaa gaagaaccaa aattggaagt ctatcgccat tatatccagc
    14701 aaatagtaga taatcgagcc catgtcttat ctgctgagca ggaatctctt cttgctggag
    14761 caggtgaaat ctttgatgct tcaagtgata catttgctgt tttgaataat gcagatctag
    14821 tttttccaac gattgaagga gaaaatggtg aaatagtcca attatctcat ggcgtgtatg
    14881 gtcagttgct agaaagcacg gatcgaaggg tgcgcgaagc agcatttaag gggttgtaca
    14941 gtgtttacga acaatttaga aatacatttg cttctacttt aggcacacat ataaaaggac
    15001 ataattttaa agcgaaagtc cgtaattaca gctctgccag agaagcgtct ttgagcaata
    15061 atcatattcc tgaaagtgta tacgatactt tggtagacgt ggtaaacaag catttgcctt
    15121 tgttacatcg atacatggaa ttacggaaac gtttattaga agtggaaaaa ctgcacatgt
    15181 atgatcttta tacaccggtc ttaggggaag ctccaattac ctttacgtac gaagaagcaa
    15241 aagaaaaagc tttagaagca ctgaaaccaa tgggtgaaga atacatggcc atcgtagaaa
    15301 aagcattctc tgaacgttgg atcgatgttg tcgaaaataa agggaaacga agcggtgctt
    15361 attcttcggg aagctatgac acaaatccat atattttatt gaattggcat gatacgctgg
    15421 atcagctatt tacgcttgtc cacgaaatgg gacatagtgt tcatagttat ttcactcgtt
    15481 cgaaccagcc ttatgtgtac ggcgactact ccatcttttt agcagaaatt gcttcgacaa
    15541 cgaatgaaaa tatcctaacg gagtatttat tggaaacaga aaaagaccct cgtgtacggg
    15601 cttatgtact caaccattac ttggatgggt ttaaaggaac agttttccgc cagacacaat
    15661 tcgctgaatt cgagcatttc atgcatacgg aagatgaaaa aggtgtgcca ttgactagtg
    15721 aatacctaag tgatagttat ggtaaattga atgcaaaata ttatggtcca gcagtcgaag
    15781 aagacccaga aattaaattc gaatggtcac ggataccgca tttttattat aattactatg
    15841 ttttccaata ctcgactggc ttttctgctg cttcagcact ggcgaagaaa atactaaacc
    15901 aggaaccaga ggcactagag aactacttgg cttacctaaa agcaggtaac agcgattatc
    15961 ctgtggaagt aatgaaaaaa gcaggagttg atatgacaca ggctgcatat attgaagatg
    16021 caatgtcgat gtttgaacaa cgtctaaatg aattagaaga attgatcgat cgtttataag
    16081 gctttctatt tttgattttc tcgaatagaa acgaaaaaaa tacgtcggct ctcttgtaag
    16141 ggagtcgacg tattttttt
//
LOCUS       DACEUZ010000056        16143 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.056, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000056 DACEUZ010000000
VERSION     DACEUZ010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16143
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.056"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(182..601)
                     /locus_tag="IXG44_001513"
     CDS             complement(182..601)
                     /locus_tag="IXG44_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HAQ1658238.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(603..1187)
                     /locus_tag="IXG44_001514"
     CDS             complement(603..1187)
                     /locus_tag="IXG44_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HAQ1658239.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            complement(1234..2709)
                     /locus_tag="IXG44_001515"
     CDS             complement(1234..2709)
                     /locus_tag="IXG44_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HAQ1658240.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPTMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            2890..3201
                     /locus_tag="IXG44_001516"
     CDS             2890..3201
                     /locus_tag="IXG44_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1658241.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(3316..5652)
                     /locus_tag="IXG44_001517"
     CDS             complement(3316..5652)
                     /locus_tag="IXG44_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1658242.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            5794..6462
                     /locus_tag="IXG44_001518"
     CDS             5794..6462
                     /locus_tag="IXG44_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1658243.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            6465..7508
                     /locus_tag="IXG44_001519"
     CDS             6465..7508
                     /locus_tag="IXG44_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ1658244.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(7745..8626)
                     /locus_tag="IXG44_001520"
     CDS             complement(7745..8626)
                     /locus_tag="IXG44_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ1658245.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERANRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            8785..9513
                     /locus_tag="IXG44_001521"
     CDS             8785..9513
                     /locus_tag="IXG44_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HAQ1658246.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            9669..9986
                     /locus_tag="IXG44_001522"
     CDS             9669..9986
                     /locus_tag="IXG44_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ1658247.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            9979..10587
                     /locus_tag="IXG44_001523"
     CDS             9979..10587
                     /locus_tag="IXG44_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HAQ1658248.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            10642..11433
                     /locus_tag="IXG44_001524"
     CDS             10642..11433
                     /locus_tag="IXG44_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HAQ1658249.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GKSYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            11571..12059
                     /locus_tag="IXG44_001525"
     CDS             11571..12059
                     /locus_tag="IXG44_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HAQ1658250.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            12110..12982
                     /locus_tag="IXG44_001526"
     CDS             12110..12982
                     /locus_tag="IXG44_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HAQ1658251.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            13158..13520
                     /locus_tag="IXG44_001527"
     CDS             13158..13520
                     /locus_tag="IXG44_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ1658252.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            13529..14281
                     /locus_tag="IXG44_001528"
     CDS             13529..14281
                     /locus_tag="IXG44_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1658253.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            14295..16097
                     /locus_tag="IXG44_001529"
     CDS             14295..16097
                     /locus_tag="IXG44_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1658254.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
BASE COUNT         5026 a         2811 c         3304 g         5002 t
ORIGIN      
        1 ccatgtttac aaaatttttc ttactactcg aaacgaagcg gctttatcac aacctcttga
       61 taaacggcgt tcaatcagct gtccctcggt attagctcaa aaacggcaaa atatgagaag
      121 ctatttttcc atttttttct ctcatactca ggtcataacg ctgatttcac aacctcttct
      181 ttcataatgg tttttcacca tctttataat aaactggttc tttgatatgg aaatctattt
      241 tttccaacca atctaaaatc ttgtaaccta actcttctga catcggaaaa gaggcgttga
      301 tttgtgcatt ggagatatgg tcaacatgga taccggaagt gatcgtacaa cttcccggaa
      361 cggcagtttt gatgatcaca gctatttttt cggctaagac atcgtcttta tggaaccgac
      421 catcgtggct agggaaacgt acagtttgca tctctgtatc acgagaaaat gtagtgactg
      481 ttccgatatg tggtgtatct ccgcctgtta aagtgatcaa taagtcttta ccgattactt
      541 taacagcagc ttggatcgtg aaacctagtt tggttacttc aactatcgtt tctttttcca
      601 tattaatcac cctgccaacg ttttgaatgc tcatttacaa tttcaaattg atccaaaagt
      661 ttcattgtat ggtgattaac aagatcttgg atcgtttgag gctgattata aaaagcagga
      721 attggtggta tgatttgcac acctagtcgt gctaatttag tcaagttttc caagtgaatg
      781 gcatttaatg gcgtttcacg aggaacaatg actaactttc gttgctcctt cattgttaca
      841 tcagcagcac gtgcaatcag gttgtcgcct aaaccaacag agatacctgc gactgttttc
      901 atacttgccg gtacgatgat cattccatcg gtcaaaaatg atccgctggc gacagcagca
      961 cctaaatctt tgtttgaata atggtaatca aacaggcttt cgaattcagt tagtgataag
     1021 tcggattcca gctttaagtt ctgttttgcc catggactga tgattccgtg tgtttctatc
     1081 atcggatatt cttttagttt cttcactagg ttgatggcat aaatggtgcc tgaagcgcca
     1141 cttacaccaa tcacgatttt tttcttccgt tgtgccaatt gttccatttt ccgcactcct
     1201 ttttatgatg tcaaaacagt tgcagatcat tcattatttc aaatattttt cccaatcttc
     1261 tacttcttgg aattttgcac gtacaaaatg ttctttcata tcgaaaggaa ctgtaccgtc
     1321 aaagatagtt tttgcactta tcccacggaa acgaatagag ccaggattat attctggtaa
     1381 ttctgatggg tccaatggat ggttcctcat tcctgggatc acgatgatat ctttatctcc
     1441 ttggaaacga gtgttgaaag tccaaactac atcgttcatg tcgaaaatat caacatcttc
     1501 atcgactaaa atgactgtct tcaattcttt gaaggctgat aaagcaagaa tagctgcttg
     1561 acgctgaata ccttcatccg cttcgttttc tttacggatt tgcatgatgg tcatcaattt
     1621 accaccacct gcaggaggat tgtagacatt tactactttt cctggaatag ctttatcgac
     1681 caaggcaaga atacttgctt ctgtaggaat accggccata gatacgtgtt cttcactggg
     1741 accaatcgtt gtttgcatga ttggattgtc ttttcggtga gtaactgctt tgactttcac
     1801 tacttgtaca gctggattag catccccagt atagccagga aattctggca ttgcttttcc
     1861 tgagttcgtg ttgatatctt cttgcattgt ttcatttggc atgatttctg cttcaatgat
     1921 gaattcagca cgagcaatac ccacttcatc gactgctata ccatcaacga tttgaatagg
     1981 ttcgttacgc aaagctcctg cacaccatag ttcattgtag ccaagtggag tggtaggtgg
     2041 ttcgaaagtc gtgccaatgg caattgctgg atctaaacca atattgatag tgataggcat
     2101 tggtttattc aaggcttcga attgtttttg gaagtgacca atgtggcgtc cgccaggcat
     2161 gatgtacata ccgatcgtat ctttatcttc tagaaccata cgatggatcg ttacatcgct
     2221 cattgttttt tctggatctg atcctaagac tacacccatt gtgatgtatg gaccagcatc
     2281 ttcaggagta tttgtaggtg caggcaaaat gttgcgaata tcgaagtctg catcggtagc
     2341 tagatgaacg acttcttgtg ctggagcgtc tgctttgtct actttcacag gtttgattgg
     2401 attttctaca gcgtctttca agaaacgacc aagtgttcga taatcatggt gcaaaatttc
     2461 acctacacgt ttacgactag ccattgttcc gatgttcaca cgtgtgccgg ggaaaccttt
     2521 gatattgttg aaagtcatag ttggtccttc ttgagtcgga cgttttactg taccgccagc
     2581 accgatataa cggtaaacac cagataattc tgcttcagga tcgatttcaa tatctgtttc
     2641 gtgatattga cctggaagtg tttttagttc ttcgatgact tttcttaaat catacggttg
     2701 atctgtcatg agagattcct ccttagttgt ctttacctat ctatagtaaa aggtgaaaaa
     2761 gaggaaaaca attcaacttg ttcggtaaag tattctacaa tggaatggat gcgttttttt
     2821 gataatagaa taaactatga tatacttatc actaaaagaa agcgcatact tagtgttgga
     2881 ggaaggaaag tgaaaaaaat ggaggagtca tttcctaaag cagtaaaagt ggaaaatata
     2941 gcgaatattt tgaaagttac atttgaaaac ggagaagtaa aatacgtgaa atctcattgg
     3001 acagaagaga taaccgatgc cttgcaattt ggaaaaaaag gaagaggaaa aagaaaaaat
     3061 cttctcgcac tttctcgtaa tatgtggatt gggactgaag taacgattga agcagatgga
     3121 acagtattca taaatggaaa agacaggtat acgccagaag agttgtggta caaaggaaag
     3181 aaaagtatac cagaattata aaaaataatt aaaaaaaaca gcgcgaaaca aggaacatat
     3241 tacttcctcg tttcttgctg ttttctacta tttcagcgct cttatgactc aaatctaaat
     3301 gtggttgtca caaccttatt ctgcacgcaa agcgatagcg gcgtcttttt tcgctgccat
     3361 tcgtgcagga agatgaccgc caagcatcgt caatatagta gagacgatca ctagaataat
     3421 cgcatggaca ggatttagtt gagcaacatt ttttagatcc gtcatgttgt ataagatgct
     3481 attgataggg aacgtagcta gccatgcaat aaacacacct aatatcccag aagaaatacc
     3541 taaaatacac gtttctgcgt caaatacgcg agtgatgtct tttttacgtg cacctaaagc
     3601 ttttagtaca ccgatttctt ttgtccgttc aatcacagat gtataagtaa tgatactgat
     3661 catgatcata cttgttacta aagagatccc tgcaaaagca ataaggacat aagtgatagc
     3721 atccatcaat ccgccagtca gttcagtcat cgttccagct aaatcagtat aaatgatttt
     3781 atcttcatct gatttgcctt tattgtaatc atctagataa tccaagatct tttctttgtc
     3841 ttcaaaattg ttcggataaa tcatgatact tgaaggcaag ctgtctccac ctaaataact
     3901 aagcaatgtt tgtttggcat tttcgtcaac tttttcagta gttaagacat ttacaccact
     3961 gtctctttga gctttgacga tttctgattc tttgttttcg ttgacgattt gagtcgttaa
     4021 ttgatcgcta taagcgattc ctggagataa gagattcatt gtagaagaag atttcacgcg
     4081 taggatcccc gagatcttca attcatcaga agcattctga tacatcgcat cataatctgt
     4141 attagggata aaattcccag ttggaagttt tgtatagtaa gtattgttgt ttactaattt
     4201 gaatgttgta ccaacgatat catcaaaatc aagtttttcg tcttctttca catcaaatcc
     4261 aagattcttc aatgcgttga tattagtatt attattgccg tctacgatca gcacgacatc
     4321 tgttgctgag gctggataac ttccggcaag taaagaataa ttgtctttta aaaagttttc
     4381 tttgctagtg tcaagctgtg tagggaaact tgacactccc acacctgtca ttgctgacat
     4441 cgtactagat aaagaaagag attccgtatc aggattttga ttcgagaagc tgacaggctg
     4501 tactttgcca tttacatcac gtaaaagatt gattcctgta gtacgggtaa atccgatatt
     4561 attacttaag ttaggatcga tgtctgttac gtaatcgacg tatgtttgat caatcttatt
     4621 cgtgtgctgt gctttatctt cttcgctgac ttttgcagta atcgttttgc tgtctggata
     4681 atccgctttg cttgcaccca atccggcatc gtcacgagtc tggttagtcg ttaccttaga
     4741 aatagtaatc ggaaatttcg ccatcgtttc agcttgcgtg ttatcgattt gtttttggaa
     4801 tccagaagaa agggacagaa cgatcgcgat tccgatgatc ccaatactgg aagcaaagga
     4861 ggtcaagaaa gtacgtcctt tttttgttcg gatattgttg aaggaaagct ttaacgctgt
     4921 ccaaaaactc atctttgttc tgcgtaagtt aaattgatca tcttttggtc gttcgatgtg
     4981 aggatgggaa tctgttaaga tcttaccatc agaaaattca atgattcgat ctgcgtattg
     5041 atgggccagt tcaggattat gtgtgaccat gatcactaat ttttctttcg ataactcttg
     5101 gatcaattcc atgatctgga tacttgtttc tgtatctaac gctccggttg gttcatcgca
     5161 caaaaggata tctgggtcat tggctagtgc tcgagcgatg gccactcgct gcatttgtcc
     5221 accagataac tgatttggct ttttatgcat atgatttgtc aagccgactc ggtgcaatgc
     5281 atcttctgcc cgttttcgtt tttcgtcttt agaaacacca cttaatgtca tcccaagttc
     5341 aacgttttca ataatgccta gatgaccaat caagttgtaa ctttgaaaaa tgaaaccgat
     5401 agagttgttt cggtaggcat cccagtcact gtccttaaag tctttagtag actttccgtt
     5461 gattaccata tcaccggaat cataattatc tagtccacca atcacattca gcatagtcgt
     5521 tttaccagaa ccactaggac ctaaaatcgc tacgaattct ttttgtctga aagcgacaga
     5581 cacaccatcc aatgctttgg tggtggtttc gccgacttta taatattttt ttatatcttt
     5641 taattgaagc ataatcaccc ttctcccgta ttgaaatatt cggtatacta ataagtatat
     5701 cagaacaatg atcgtagtat acgaaataaa tgttaactga agatgaacta caagaaaaaa
     5761 tctagaaatt tttcaaaaaa cagagggaga tcaatgaatc gtattttagt agtagaagat
     5821 gatccgcaat tacagttgct ttaccgttct gtgttggaac gagcgggctt ttcagtcatc
     5881 accgcattga atggagagga tgcgctgaaa aaactagaag cgacacgtat agaattaatc
     5941 attacagata tcatgatgcc gaaaatggat ggctatgact tgctagactc attacgtagc
     6001 accagatttg acacacctgt tctaatgatt acggcaaagg ccgattttga ggataagaaa
     6061 cgcgggttca aattaggagc agatgattat atgacaaaac ccatcgatgt caatgagatg
     6121 atcctacgtg tggaagcatt gcttcgacgt tcaaagatca attacagcag tagcttgata
     6181 atcggacaaa caaaattaga gctagatgct tatacagtgt tacaagatca gcattcgttg
     6241 attctgcctc aaaaagaatt tctgcttctg tataaattac tgtcttatcc caacaagatc
     6301 ttcactcgcc aacagttgat ggatgatatt tggggattag atacgaatac agaagaacga
     6361 acgatcgatg tacatatcaa acgtttaagg acccgttttg aaaagacgga agattttcaa
     6421 atcatcacag tacggggact aggctataag gcggttattt aaagatgaaa aaaaagctat
     6481 tttcacaatt atggatctat tttgctagta ttgtcttcat ttcgatctta gtgacactgc
     6541 tgtgttttct cggttttatt ttcttattgt ctcgacattc tatctcgcct gctgaatcgg
     6601 ctggaaaacc aacgttatac ccgttattcg tctttgcggg gttcagtatg gtcgtgggta
     6661 caggaatatc gatttttgtt gggcggcgta tcctgcatcc aatcagtgcg ctagggacaa
     6721 atatgagtct ggtagcaacg ggagattttt cgattcgaat ggatgaacag cagaaagtag
     6781 cagaagtaca gcaactgtat aaagatttca acgttatggt ccaagaactc aatagtatcg
     6841 agacactgag gaatgacttt gtgtccagtg tatcccatga attcaaaaca ccactagcaa
     6901 caatccaagg atatgttcaa ttgttacaag ccccaaatct ctctgacgaa gaacgccaaa
     6961 tatttttata tcggattatc gaaagtatca cgcagctttc acaattaaca gagaacatct
     7021 taaaattgaa caaactggaa aaccagcgta tccaactgga aaagaaagag tatcgattag
     7081 atgaacaaat cagagaagta atcgtttttt ttcagcctaa atgggagaaa gaacagctag
     7141 aattggatat cgaacttgct gcagtaaatt acacaggcaa tgaagagttt ttgtaccaag
     7201 tgtggctgaa tatcatggat aacgccataa agtacaatca aatcaatgga cagattcata
     7261 tcaaactatt tgagacagcc acagagattg tactggaagt gaccgattca ggtgtaggaa
     7321 tgaatgaaga gaccagagat cgaatgtttg aaaaatttta tcaaggagat acaagtcgtc
     7381 aaatttctgg gaatgggcta ggactttcac tggttaaaaa aattctagaa ctgcatgatg
     7441 gacgtattga ttatagcagt atcgaaggag taggaacgac agcaatgata cggttaagta
     7501 aacaatagga aaaagaataa aacccctaaa aattctattg aaggttttta ggggttttta
     7561 gtcgtttttt tgtgattagg gaaatgttct tacggttctt ttggcataaa aatcgctgct
     7621 ttcttattga actacgagaa atgttagagg tgtcattgaa acaaggacat gatgcaagaa
     7681 agagagcgaa agtcatgcgt cgctctcttt ttttgggtgg atagatggac agaaaatgta
     7741 gggcttagtt ttcttcttga tttaaatact cccaaaatgt atctgaagcc aaagaatgct
     7801 caccgacttt acgagtaaat gcaagataac tctctgttgt tggagaaata gggagaatca
     7861 cagtattttc ttttaataga ttcttgactg ttttttccat aacaatagca atccccatgt
     7921 catttgcaat catattgatg atcaagtcga ttcttgctcc tttataagag atttttggtt
     7981 caaatccagc ttcgcgacac agatctagta caggttgata caagttcgta gtggggccaa
     8041 ggagcaaaaa gttctccttt ttcagttcgc ttagatccaa tttcttttta ctagctaaag
     8101 gatgattttt aggtagaact gctacaaaat cgtctgtttc agtaggcaac cactcaatcg
     8161 aacttggtgg ggcctggaaa cttctagcaa agtggatcgt gtcactatgt tcattggcgt
     8221 cattgtaagt taattcgaca ctttccactt ctttcaactg gactgtaaat tcaggatggc
     8281 gtttcaaaaa ctcggtgatt ttggaaaaac ctttataatt gatcattgtc ggtatcgtta
     8341 agatatttaa tgaaagattg ttgtctttcg tataggtttc aagcacttct tgcatagcat
     8401 ggtattgttc tacgacattt tttacatatg ggagcaataa gcttccagct tcggtcaatt
     8461 cgatttttct attggcgcgt tcgaataatt tgacgtctaa ttctgtttct aacgaaagaa
     8521 tctgtttaga aatatttcct tgggtggtga attgtcgatc agcagttttg gtgaaattca
     8581 ttgtttcggc taaatcaaga aaaacttcga atttatggat atccatcata ttcctcctac
     8641 ttattccaat aaagaatact ttaacacaaa aaatgaattg gtgtaatctt ctctgtctgt
     8701 tacaatcttt cttgcagcga aagcaataat tacagaaatt tttctgattt ttttcgcttc
     8761 gtagaaaaag agaggagttt tttgatgcca actggagttt tgatcaatgt aggttccgtt
     8821 ttgtttggcg gactaattgg cggattagta ggaaataaat taagtgaaca ttttaaagca
     8881 cagctgacga tggtttttgg tgtgtgttcg atggggatgg gaatctattc cattgcgccg
     8941 atgaaaaata tgccggctgt tatttttgct ttagtgatag gaacagcgat tggtttgatt
     9001 gttcatttag gtaatgggat aaataagggg gctgcgctga tgcaagtacc tatttctaaa
     9061 atcttcccta gtgaaaagct tggtatgacc cacgatgaat ttatcagcac attagtgacg
     9121 gtcattgttc tattttgtgc aagtggtacg gggatctatg gaagtttaga ttctgggatg
     9181 acaggagaca gtacgatttt gatttctaaa tctgtccttg attttttcac agcagctatt
     9241 tttgcgtgta atttagggta tgtcgtttct gtgatagcaa ttccccaatt cattattttt
     9301 tatattttgt tcttattggc aaaatttatt tatccattga ctacaccaga catgatctta
     9361 gattttaaag cttgcggcgg attcttgatg gtcgctaccg gttttagaat gatcaacgtg
     9421 aaaatgttcc cagtagctga catgatacct gcaatgatcg ttatcatgcc actgagctgg
     9481 atgtggacaa attggattat gccactgtta taataaaaat caaagagaag ccattttttg
     9541 ctgactaaaa aggatcaaat cttttagtca gtttttttgt atttgacaaa ctgattttga
     9601 aactatatta tatgttgtat aatatagatt gaaataaaat attacacttg agaatagaag
     9661 gtgacgtaat gcacatccag attccaactg ttcttcttga tggtactgtg ctagcagcat
     9721 taagcaagga agatatgtat gggtatgcct taacaaaaat cgtacaagaa agtctctctg
     9781 tcagtgaatc tacactatat ccggttttga gaagattgaa aaaagaagga tatcttgaga
     9841 cctatgatga accttttgaa ggcagaaacc gtcgatatta ctcgttgaca ccaatgggaa
     9901 gtcgtcattt agaagaaatt attgatgaat ggattgtttt tcgagattct ttagaccggt
     9961 tattggagga gagcacaaat gaatgaatat ttgaatgaag ttgtggatca tcttcaagaa
    10021 attcctgaag acgatcgttt tgatttgatc cagtattatg aggaatattt tttagattct
    10081 ggaaaaacat tagatcagat cattgatgaa tatggtacac caaagcagtt tgccttaaaa
    10141 ttaaaaatca gttacttttc agaagaagaa caagcagaaa acgagttagt gaaaaatcca
    10201 aaaagacaaa ttcgcctgat ttggttaatc attatcggtt tatttgcttc tcctgtcctc
    10261 attccactag cactggggtt tatattggtt attgggggac tattggttgc tttactagcg
    10321 ctcattttta gtatctatgt tttatgtatc agtatcttag gtgttggcct attcatgatc
    10381 atctcaggaa tcagtgtgtt tggacaatcg attctgagtg gattattctt tatcggtgtc
    10441 ggtctgttcg ccacaggaag cgcagtattt atttcgccac tgttgctgaa aggaacaaaa
    10501 gggctttttt atgtcatgat gaactttgtt aaatggatgg gacgacgttt tattacaaaa
    10561 agagatctgc attcagcggg cgtttgaagg aggacacata aattgagatt gaaacatttt
    10621 ttgattaccg gtattggtct gatgcttact ggtggcctcc tagcaggtgc aacttataca
    10681 ttaggcgctc aaaagagtct gacttgggat aatggtcctc ggttgatcga tatggttact
    10741 gagacaaaaa aaatcaagga tgcaaacaaa atcgtcatcc atgcagaaaa tcaaacagtt
    10801 tcggtcatac gcggattgga ttttgagata aaaacgactt atgataagac agaaaagcca
    10861 gaaataaagg agaaaaatgg cgtgttgact gtgaatgcag tcaaaaaatc aacaggtgca
    10921 atcattagtt tagaagacac aagtgcacaa atcactattg ttgtccctgc agatcatgaa
    10981 ttatctgaat tgacaatcga agggaataat agtgcaattg atatcaacga agtatcaatc
    11041 aaaaaagtga atactaagac acatgatggc tgggtatcgt tcaatgaatt tacaggagga
    11101 acaataactg cgaaagatga attaggctca cttcgattag atgaggccaa tgtggataac
    11161 ttgatcgtca caggtgaaaa cagcaacttc catctagaag acagtttgat cagcaaagaa
    11221 ggaaaagtca tcctaaacgg tggcaattta tctttgcatg agatggaaaa cagtggctat
    11281 actatccaaa cgtctggtaa atcttatatt gaaaaagact accagccaat aacaaatact
    11341 ttgttagaac aaggacagaa aaagctaaag atcactgcta aagacacatc gatcaatatt
    11401 acgacggatc aagaggacaa tgaaacagat tgatcctgta ctcaactgga tattttaaga
    11461 aaaaacagca aagatccttc caatctttgc tgtttttcat cgcttaacgc acataactat
    11521 agtaaaataa gaaagaaaat atctaaaaag aaaacagggt gaaagagtat atgaaggtag
    11581 aagaaggtca aagagaaaag ttaaaaacag aaatggaaag attacatact tatatcactc
    11641 aactaagcca aaccttctat gatccagaca aagaaaaagt gatggttaat tacccgaaca
    11701 attcagaagg tcgccaatta gagcaagtgt accatgaagt tttcaaacat ttattgacgg
    11761 tgaagaaaga gctggattat tattctttac cgattatcga tacaggaata ttgaagtatg
    11821 atgggaagaa ggaacgattt atctttaaat ctgttcggga agatttgcct ctttcagcag
    11881 ggatggatct agaggtcttg gtagaagact attttacaga agaaaagcac tgggttcgaa
    11941 caaaattgga ttatttaccg caggcagccg gagggacaca ggcaagcggt tggtacatca
    12001 ctgaagacaa agagttagaa ctagaaggtg taatggcgcg tattcgaaaa aaaaattaaa
    12061 aaagtaacat tagatattta ttgaaggata aaggagcgca ggaagtagca tgagagaaaa
    12121 aagagcattt tatatcaatg gttatgcagg gattgtcgga ttggtcgtat tagctttagt
    12181 tggattgttt ttcttctatt taggtatgtg gcaagcgaaa gtttgggcac tttttttaag
    12241 tatttttctt tggttgatca cgttattgtt gttaagttcg gcaactgtag tcagtcctaa
    12301 tcaagcaaaa gtaattttgt tttttggaca atatttaggt acgattcgag aaaatggctt
    12361 tttccttacg atacctttgg cacaaaaaat gactgtttcg ttgaaagttc ggaatttcaa
    12421 tagttctgtt ttaaaagtca atgatttgga tggaaacccc atcgaaatct cagcagtagt
    12481 agtctttaaa gtcgttgata cagcaaaggc attattcgat gtagcgtatt atcaagattt
    12541 tgtagaaatc caaagtgaga cagccattcg gcatatcgct agccaatacc cgtatgatac
    12601 attcaatgag aatgatttga cgcttcgagg aaacacgacg gcagtgtctg acgagttgca
    12661 aaaagagctg caagagcgtt tagcagtagc aggtgtcgaa gtaatcgaaa cacgcttgaa
    12721 ccatttagcc tatgcaacag aaatcgctag tgccatgctc caacgtcaac aagcaaaagc
    12781 cattttgtct gctcgacaaa ccattgttga aggagctgtc acaatcactc aaatggcctt
    12841 agagcaaatc gaagatggac aagaaatcaa tttcaccgat gatcggaaag tccaattgat
    12901 caataattta cttgtctcaa tcattacaga caaaggaacg caacctgtta tcaatacagg
    12961 cgacatgaaa gaccgtaagt gacatacttt ttgtaagata ggaagaagga gtttaggacg
    13021 gattattgtc ttgaactctt tttttagttg ttttaaagaa aatgacaaaa ttgaaagaag
    13081 aatgatagtt gattcgaagg cagaattgtt cgcttttttc tataatcagt atataaagta
    13141 agagagaagg ggaaaaagtg aaaaaacttc tgatcggtct acttgttttc atcgttgtgg
    13201 tacttgcagg ggtgaaaatc gcagattacg tagttatggg tggagaagaa tactatgtac
    13261 aaattacgac agatggtaca aaaacagttt caaaatcaga ctctggacaa gaaacagtca
    13321 actatagata tatcctgcct ggctatacga aagagggtaa ggaaaaagaa ctggacttca
    13381 agggacaatt gactcgaccg cttcgtaaag gagcatattt aaaaatcacc tggaacaaaa
    13441 ataaaggtgt cacttcgtat gaagaagtcg aaaaaaaaga cgtgccgaaa acagcactgg
    13501 aaaaactaat taaagggtag ggaaagaaat gaacaaaata gtcgaagtac agaatgtaac
    13561 aaaagtatac ggaaaaaaca atgagaaaaa gacgcaagca ttaagcggca tcagcttcga
    13621 tgtagaaaaa ggcgaattta ttgggatcat gggcgcttct ggttctggta agtcgacact
    13681 tttgaatatt ttatccacat tagataaacc aacagatggg catatccgaa tcaatcaaaa
    13741 agatgtgacc acattaaaag gaaatcaact agcagatttt cgtgcgaatg agattggctt
    13801 tattttccaa gatttcaatt tattagaaag cttgacagca caagaaaata tcgctgttcc
    13861 tctttcttta caaggcgtca gaccaaaaga aatcaagcaa cgtgtccaaa aaattgcaga
    13921 acgtttatcc atctctcata ttttggaaag ctatccttct gagatttccg gtggacaaaa
    13981 acaacgggtc gccgccgcac gtgcattgat cacacaacca acgattctat taggggatga
    14041 gcctacaggt gcgctggatt ccaaaagtgc ccgggatctt ttagatacga tggacgaatt
    14101 gaatacaaaa gatcatgtat cgattcttct tgtgacgcat gatccgtttt ctgcaagtta
    14161 ttgccaacgt attttattca tcaaggatgg cggtatccat caagaagtga aacgtggaga
    14221 tcaaagccgt gattccttct atcgagaaat actatctatt cttggaaact tggaacagta
    14281 gggggagatt gactatgctt tggaaattat cacttactgg gatcaagagc cgtttaagag
    14341 actacttggt gttattctca ggattagtta tggcatctgc tattttttac atgtttgaat
    14401 caatggcaag caacgaagct tttttgaaaa gtaatacgat cgttgcggct acagttatca
    14461 ttttccgatt cggttctgtt ttactaggga tcatcacatt cgtttatatt ttatatgcaa
    14521 attccttttt gatgacgatg cggcaaaaag actatgcaat gtttatgatg ctgggagcaa
    14581 aaggcagaaa aatcgcacag atgatttttt ctgaaacatt cttagtaggg atcgcagcta
    14641 ctttagtggg ttcagctgtc ggtgtaggat tgacaagtat cgtccaccga ttattagtgg
    14701 atcagttaga tctcaatatt tcgcatttta caccttttag tattcacggg ttgttgatca
    14761 ctgttttgtt ttttgctgtt ttgtttttat tagctgctgt tgtcaatgct ttttcgattg
    14821 tcaaaaaacc gattttagca ttgattcgtg cggatcagac accaacaaga atcaagcagc
    14881 ataaattctt gtttttcctt gaagtagtac tgggaattgc ttttctaggc tgcggttact
    14941 atatgatgaa acacgtgggg acctatcaag tctttggtat aggggtagcc ttagttacga
    15001 tcgtattagg aacttatttc attttccatt ctgtgatcat tttcttttta tcattactaa
    15061 agaaatcaga acagatttca ttgaaaaaat tgaacaattt cacattgtca cagttaaact
    15121 ttcgtattcg tgaatataca caaatgctct caatggtcgc tatgctgttt gccttggcac
    15181 tgggcgcttt gacggtagga ttaggtttta aaaatgaaat tgcagacttg acagaaaaca
    15241 attctggata tgatctagtg atgaataatc ctcgggcaga agatcagcaa aaaatcaaag
    15301 aactttcacc aacactttct gcttcttata ctcaaaaaga agatgcggga acgatttact
    15361 ataatgcaag tgaatttgcg gctcaaccat tgatcatgat aaaacatgaa gatggaacac
    15421 cgccgaagat cacgaaagaa aaagttcctg ctaaaaaaat gaatgaactg actgtacaag
    15481 aggaacttcg aagctatgaa ttgccggaac agcaagaaaa agaaatcaag gtagtcagtc
    15541 aatcagaatt tgatcaactg aacttgccac aatccacttt acagctagtt caggtaaaag
    15601 acttccaagc aaacctgaaa ccaatcgaaa cacttgttgc tcaaaataaa atgaacaatc
    15661 catctttgca aaaagtagag tacagcaacc agaaatatga aatgtatgaa gtgtataatg
    15721 gtctattctc tggttttgaa tttatgggat tcttcctagg tttagctttc ttgaccatgc
    15781 ttgctagctg tttgatgttc aagatcttgt caggaagcaa gagtgacatt gctcgttatg
    15841 aaatgcttga aaagatagga acacgacgcg ggttgcttaa acaatcgatc cgtagagaag
    15901 taggcgtgtt attcttagca ccaggaatct taggtgtagt ccatgtctta ttcggattac
    15961 aaatgtttgg tctattcatg caaaatccat acaaagatat ttgggttccg tttacgatat
    16021 tcttcgtatt gtatttcatc tactatgtac tgacgacatg gttatatact ggaatcgtat
    16081 tgaaaaagag attgtgaaaa agctgtcctg catcaagtaa taagaacagc caagcaaaaa
    16141 tag
//
LOCUS       DACEUZ010000057        16109 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.057, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000057 DACEUZ010000000
VERSION     DACEUZ010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16109
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.057"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            161..598
                     /locus_tag="IXG44_001530"
     CDS             161..598
                     /locus_tag="IXG44_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1658255.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            693..1577
                     /locus_tag="IXG44_001531"
     CDS             693..1577
                     /locus_tag="IXG44_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAQ1658256.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            1801..4968
                     /locus_tag="IXG44_001532"
     CDS             1801..4968
                     /locus_tag="IXG44_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1658257.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            4988..5533
                     /locus_tag="IXG44_001533"
     CDS             4988..5533
                     /locus_tag="IXG44_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HAQ1658258.1"
                     /translation="MNRIWNKSHRNNCSLLMILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILI"
     gene            5561..6016
                     /locus_tag="IXG44_001534"
     CDS             5561..6016
                     /locus_tag="IXG44_001534"
                     /inference="COORDINATES: protein motif:HMM:NF023225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
                     /protein_id="HAQ1658259.1"
                     /translation="MKLKKAYADQSDGRNVIKANLQNPNASYMFGLTTSVEIYKKETK
                     EPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKGAWKEKRWNLHTDFEITD
                     DAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFILKERRKKK"
     gene            6013..6771
                     /locus_tag="IXG44_001535"
     CDS             6013..6771
                     /locus_tag="IXG44_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAQ1658260.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(6857..7597)
                     /locus_tag="IXG44_001536"
     CDS             complement(6857..7597)
                     /locus_tag="IXG44_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1658261.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            7658..8911
                     /locus_tag="IXG44_001537"
     CDS             7658..8911
                     /locus_tag="IXG44_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ1658262.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            9233..9460
                     /locus_tag="IXG44_001538"
     CDS             9233..9460
                     /locus_tag="IXG44_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAQ1658263.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            9583..9909
                     /locus_tag="IXG44_001539"
     CDS             9583..9909
                     /locus_tag="IXG44_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAQ1658264.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            9977..10690
                     /locus_tag="IXG44_001540"
     CDS             9977..10690
                     /locus_tag="IXG44_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAQ1658265.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            11000..11851
                     /locus_tag="IXG44_001541"
     CDS             11000..11851
                     /locus_tag="IXG44_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1658266.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            11972..13837
                     /locus_tag="IXG44_001542"
     CDS             11972..13837
                     /locus_tag="IXG44_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1658267.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAEADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHRGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            13885..15294
                     /locus_tag="IXG44_001543"
     CDS             13885..15294
                     /locus_tag="IXG44_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HAQ1658268.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            15402..16103
                     /locus_tag="IXG44_001544"
     CDS             15402..16103
                     /locus_tag="IXG44_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1658269.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
BASE COUNT         5375 a         2705 c         3450 g         4579 t
ORIGIN      
        1 tttttattag aactaaatca acagtagttt aattactact tgatttagtt ctttttattt
       61 taaaaatttt ttttacttag aatccagcga ccctagatat ttttctgtat aatcttaaat
      121 aaagaaaaca atatcaaaaa atgcacgtag aagtagataa atggagtggt tttgcaaatt
      181 tcttttattt ctagaagtta ttcacgtata cggatttgtt tttctcacaa ataaaaaggc
      241 aggaagtaca atggatcatt tgtatataaa atatattcat caattttccg ttttggtgtt
      301 aggcacacta attatagcgg ggattgtcgc atttattatt caaggccgtg acatattaac
      361 agtacatcaa gagtttttca gttatgcatt tggtattcct ctgatcgcta taaggtctga
      421 taacaaaaag tggaagaaac gctattacat tctcttgcta tttttgtgtt tttatctgcc
      481 acctaagatt tttactttat taggtataca atgcctatca ttttctttta tttcctatat
      541 cattgtttta attttcctca gtttcatagt atggatgttt tatcttgtca aaaaataaat
      601 gaatgtttca tgaacagtac ttcatcacag cctaattttt ttctttatgc tatcgttata
      661 ttaaagaaat caatcagaag ggaaagaaag tgatgaaaaa agttttatta gtggtaaacc
      721 ccagttcagg cggtgaacaa gcaaaggaat ttgagcaatt agcgatagca aaattggaat
      781 cggtgtttga tgaagtggtc gtcttgcata caaaaaaagc aggggatgca aaaaacttta
      841 ctcgcgaagc ggctacggaa gggtatcata gtgtgtttgt catgggcgga gatggaacag
      901 tcaacgaagg aatcagtgga atcgctgaac aagaacatcg gccaaatttc ggatttttcc
      961 cattgggtac ggtgaatgac ttggctcgag cattagggat accgctagaa cctgaggaag
     1021 cgatcaatca tttttcgatt gaatcagtca agccattgga tattggaaaa ataaatgacg
     1081 actattttat gaatgtcgtc gcaattggct ctatccctga atcaatcaat gatgtagatg
     1141 cggagaagaa gacgaaattt ggaaaactag cttatttcat gtcaggaatc aaacagttag
     1201 cttccacaca atcctactcg tttcacgtag aagttgatgg aaagaaggaa gaaattgaga
     1261 gcagtacctt gctgatagga ctaactaact cagtcggcgg ttttgaaaca ttacttccaa
     1321 atgcaaaagt ggatgatggt aagctccatt tggtttattt aaaagattct tctttgatcg
     1381 atacgcttaa aacattgcca gacctattaa aaggagtaga tgaatctacg aaaaatctag
     1441 cctaccttac ttgtgagcaa ttagttgtgt cattagctga tgaggaagaa ttgacgacga
     1501 atgttgatgg agatgaaggg gataaattac ctgtgacaat caaagtattg ccctcacatt
     1561 tacaggttta ttgttaaaat ggaggttgtg accgaggagt tggcgggaga acaccgcttg
     1621 ttcgggataa gggactgtga caagactttt gtcacagcct tttttttgta caaaaaagaa
     1681 atttacacct aatagaaaaa tttttccata aaaatacata ttttaaatat gaaagtacaa
     1741 ttggaaatag attaaaacga taaaataaaa ttgtcaaaac gtcagagagg atggtggaga
     1801 atgaatcaat gtgtaaaaaa gctgattgcg tcgattttgt ttgtcttccc tctcttgatg
     1861 gtgacatccg tttttgccga tgtagagaaa acaaacgaat caacgcaatc agagaatcaa
     1921 agttttgaca agactacttt ttcagaagaa tcgattactt ctgaaactac agaatctcaa
     1981 agtgaggctt ctactaataa aaggcaagaa gaaaataaga caagtgagac ctcagaagta
     2041 gctgaacaaa cgtctgatag cagtgaagcg gcactggcaa gagctgtttc agcggatggg
     2101 tacagcgaag ctgccacaac agaagaactg gcgcagcttc tcgcagacga atcagtaact
     2161 aagatccgac tgattcagcc tttgacttta gacagggaat tagagatcaa acgagatatc
     2221 gttattgatt ttggtggatt cgctcacaat ttcggcacac accatattta tatcaatgag
     2281 atacctcagt cgatcgagtt ccagaatttt aaaggaacag ctgcacatcc aggtggattg
     2341 acaccaaata tcgatggaaa tgccattatc attgcttata tgaccgattt ttggggaagc
     2401 agatatcatt ttacgggaga aataaaattt accgggacac ttgatttgta tgatgggtcc
     2461 aaattaggac tgatttacgc tcctcgtgca actgtcacat tagatggtgt ttccggtgtg
     2521 ttggatgtcc aaccagtgaa ggaaggactg aatgccgctc cgggtaaagc ctatttttgt
     2581 agaagctatc aattgaatgt gatcaatggt agtcaacttt atggaccgta tctagggaaa
     2641 ttctacggtt ttctagattc aggagatgag actggtagtg gaaaaacagc gccaggaatc
     2701 catatcttaa gcggttcaaa ggtctctctt gattatgatc gggcagatgg cgttagcgat
     2761 gaaggagagg cagtcgatac gctacctggt aatgtcatgt ttgaagtcag tggttcggga
     2821 agcgaatttt cggtaaatac aaagatcaat attaccaatg acaacaatcg cggaatcatc
     2881 caaatgagag gttcaggatc gaaagtcact gtatcgaatg gtgggaaaat caccatcaat
     2941 acagcaacta cgggcggatt tcgcttgcaa ggagattcta gtcaaatcca tgtatcaagt
     3001 ggtggacaaa tctcggtcaa aatggctgga gatgacgatc aacccgggaa caatgggatg
     3061 cgttttgtag gaaaaggcct ttctttgatc gtagaaggag ccggatcagt tatcgacatc
     3121 gataagcaga gtgggcgttc cagcgcagtt cgttttgaga atggtacgca aaagctgaca
     3181 gtaaccaatg gaggctcatt gagggtaaga aatacaggtg atggcaaatc ttacgtgaac
     3241 agactcaatc aaggaaatca agcgatccaa ttttatgacg cttctggttt tctagaaagt
     3301 ccaggttcag ctgattttac agtcactgga gaaaaatcaa atatcgatat acgcgcagat
     3361 tccggagcaa ctgtagatac aactggagat attgatttaa attttacggc aggtgaaaaa
     3421 acttatttag tcatggtcgg gaaaacaggg gaagataaga ccggcatcat cagtggggat
     3481 gtgttgaatg tggacttaga atctcctacc tattttgatt tccaaaacaa acggattgga
     3541 agcgagacca atggcatagg cggctgggta ttccaaggaa atggagattc ttctttaaag
     3601 atcgcccatt cagaaatcgc tttatggcga aaaacaggcg caacattcga taactttgaa
     3661 aaagacccgg attatttttc tgatatgaca gacttatcga tcaatgggac cgacttaggc
     3721 aatttcgtat caagccctag tgcaggttta acaaatgaat ttgctcagac aggtgccgga
     3781 atgaaaaatt ataatcgaat cagtgccaat aatcagctcc cgatgattga atccttaaga
     3841 gtgccaacga atgcagataa aaaaatctat gggcatgtcg ttgttcccga aggtgtcgag
     3901 gcaaagccaa gagatgcttg gacaggcgaa gtagaagtga ctttacgtat tacttacgca
     3961 gatacgacga aatcatctgt agaaattact gctctcacgc aaggacaaac agatgaaaat
     4021 gaaggggcag gatataaccc atggggagaa ggcgaacaag gcggcttgtt cgaagttcaa
     4081 gtacccaatg atgagagatt aaaaacggag gatcaagtgc aagttgttgc tgctaaaaaa
     4141 gtaagaggtg gccaagaagt gacagaactg gactctgcca aaacgacagt cgatgtgatt
     4201 cctcctgaac ctgctaattt aagtaccaca ttgatcaatg cagcttctcg cacgatcaca
     4261 ggtacaggtg aagcaggagc cttggtttca ttgaaaaaaa atcaagctgt tttgtcagaa
     4321 acaactattg atgatacagg ggaatttgtt ctttctatcc cagaaaatca actttttgca
     4381 ggagatgtat tagaaattgt attaaacgac caagcaggag aagctactgc taagggtgtg
     4441 cagaacaagc caagtaccaa cgatgaaatt gggaatcaca atccaagtga tacgccagtt
     4501 gtttatcatg atgccccgcc ttttgcacct gctacgaaga tcgtagtaga aggtggtctc
     4561 tcatttcttg ccccttccaa actcgatttt ggtcagatca aagcaactgg tttgacacaa
     4621 gaagcatttg gccaaatgga tatacaggaa aaattatgga tttatgatca gcgagaaacg
     4681 aaaaatccgt ggatcttaac gataaaatta tctcagcctt tcacaaatga tgcaggattt
     4741 tctatgcagg atgtcctata tttcaagaaa ggagaggagt atcaattagt gaatgaagaa
     4801 gaactggaaa ttgtcagagg acaaaattca acgaatgtgc aagcagatta ttcagaatat
     4861 ttaaataacg gtcgggcgtt taaacttgtc ttaaccaaag aaaatcagat agctggtaat
     4921 tactcagcag agataacatg gacattagca gatgcgccga cagaataaag atcaaggagg
     4981 atgattgatg aatcgaatat ggaataaaag tcatcgaaac aactgctctt tacttatgat
     5041 ccttctcctt ggattcatcc tttggcagac acctgtccat gcctccgaat tcaattttgc
     5101 ggtagatttt gaattaccgg ctaatcagat tgataagaac aaaacatata taaatgtaag
     5161 gatggagcct gggaaaacac aggaaatccc ctacacggtc tttaaccgaa cagataaatc
     5221 agtaacagtt gagacattga tccactccgc tacgacgaat tcaaacggtg tgatcgaata
     5281 tggagaatca aaaagtaaat ttgatgaaac attaagttat gatatgaaaa acttagcaaa
     5341 aacagtggga gaaataactg ttcctcctca aagtgcggtc actgagacac tttcactgaa
     5401 agcgccagaa aaagagctgg ctggtgtggt cgcaggaggg atcacgtttc aagaaaaaaa
     5461 agagaacgtt tctaacaaaa gtacagttgg tgtcgtcaat cagttttctc aagtgaaagc
     5521 aatcttgatt tgaaatagcg aaaaagagtc ttccccagat ttgaaattaa aaaaagctta
     5581 tgcggatcaa agcgatggaa gaaatgtgat taaggcgaat cttcaaaatc caaatgcttc
     5641 ttatatgttc ggcttgacta catcggttga aatctacaaa aaagaaacaa aagagcctta
     5701 cctttcaata aaaaaagaaa aaatagatgt agcacccaat acaaattttg atttacttgt
     5761 cccattagaa ggaaaaaaat tagctccggg acattatcaa gcggttgtga agggtgcttg
     5821 gaaagaaaaa cgctggaatc tccataccga ttttgaaata actgatgatg cagcggacca
     5881 attgaatgaa aaggatattg atttaaaaaa tcagccagaa acatttgact ggcgatggct
     5941 gatacttgtt ggagtcattc ttattggctt atttgctgga ttatttattt taaaagaaag
     6001 aaggaaaaag aaatgaaaaa aacaattgca ttaagtaccg ttctagctgg aagtctatta
     6061 ttatgtacac aaagtgtttt tgctgagaat aagagctcag ccactacaga aggaacgatt
     6121 tctgtgacca aaccagatgc tgcaaatcct gaagacgttg ttccagttcc tcctgttgat
     6181 ccaaatgatc ctgatactcc ttttacacct gacgaaccaa atcagggaac atccggatta
     6241 ctgacgattg accaagctcc agatcttgat tttggaacaa tcaagttagg tacagcaaaa
     6301 acagtatatg ctgcattaca aagcggaacg gacagtactg gagcacaaac acaagtacag
     6361 aatttcgtac aagtaacgga taaaagcgga aattatgccg gttggaaatt atcagtcaaa
     6421 cgaacagaat ttattggtac aacatctgat acgaataaac taactggttc aaaggtgaaa
     6481 ttcaaaaacg ccgtgattcg aaatagcgca actaatagca gtggaatgcc atctaccgtt
     6541 tctggaactg gcgctgaagg aatcgaaatc ccagcagatc aggcagtaga tctagtaact
     6601 gcaggagcaa atgaagggat tggcacatgg atttattcat taggagagaa tgtggaacaa
     6661 gggagacaat cagtggaatt atttgttcct gtatccaatt atgctgtaga caactacgtt
     6721 tcaacattca catggtcgtt aacagatgct cctacagaag aggaagcgta aaatcatatc
     6781 aacgatttca gggagtggag ccgattcaag gcccactccc tgaaatctgc tatgcttacg
     6841 tctcagctct tattcatcaa ctcgaagcag ttttttggaa gtcatggaaa cgttcctttg
     6901 tcttttcaaa gatctttcga ccaacgagta aggagtcacc gttgaaaaat tctaacacat
     6961 aatgagcaga atcaattttc tgcaattgcg ataagttgat acagtaagat tttaaaagta
     7021 aaaagtaagc atacttttct aactctgtct tcaattgttt taaagaagta ttcactaatt
     7081 tatcttctgt agttgttttg accataactt ggtttttcaa acctttgata ctttcaatat
     7141 ataaaatttc ccgaggatac aggacgagac ttccaccttt ttgatcaata aagattttat
     7201 cgacatttac gatctgctgt tctttccaat tgatctgttc tagcacttga cgaatttgtt
     7261 cagataaatt cctgtctttt ctgatatatc caatcggaag gattttccga ttgatgatct
     7321 ctactgcaaa attcgtataa gcggtgaaaa aaacaatatt actatgtgca ttttttttac
     7381 gtatttgttc ggcgacatca atacctgtga gtgattgctt caagtcgata tcgataaaga
     7441 aaacatcgtg attatcaata gtgcccaact ggatcaactg attgatttga gagatagaag
     7501 tgagctgcac gatattctcg atatgatgat tgaccaattc tctgatgatc cattcgctgt
     7561 gaaactggtt atcttctatg ataaaatact ttttcatatg caggctccta ttaaaaaaat
     7621 atgtgtacat tttgaaacaa aacaatggag gaataagatg gtcgcaacag tgacacttta
     7681 tttgaccttt actttgattt tagatttagg tatctattta ttcttttcga aacaactgct
     7741 agtaaaaaac agctttggtt gtatagcttt gatggtggtg acacttttac ttcctatccc
     7801 taaagtgagc tttccttacc tagttggctt attattgatg tttctttttt attttactac
     7861 gaaaaacctg tttatcagct gtttgatcag taatcttttt tttgctatca ctggactcag
     7921 cttttttctg gtttttgatc tgatggatta tttaggaaag agacatcttg gaacagcaag
     7981 tttttttgca ctacttatcg catttatttt ctatctatta gtaaaaagta tagacaaaca
     8041 gacgagattt ctcaacttgc tttttactta tccaactgaa cactattttg gaggtatttg
     8101 tattttatta agctggggcg tgatttactt ggtattggga agtgttcact tccgctcatc
     8161 taattatctg cttctatctc ttttgtcgat agtcatcagt tttttttctt tgtttttttc
     8221 cattatgttg atcagtttcc aagtaaaaga aagacaacat caggaatcgc ttcaactgta
     8281 tcaaataaat gaagaatatt accatcatct agaggaattc aaacatgatt ataaaagtct
     8341 actgttttca ttaaaaatta ctcttcagga aaataaaaaa gaaacgttgg aatttttaga
     8401 aatattagaa gatcattctg cagctatttt tgaaaaaccg caaatacaac agctaacaaa
     8461 tattgtatcc ccagctgtca ggggtgtctt tttaaaattt ctagaaaaag cagaatcgga
     8521 gaaaatcccg atccaagtag ttattcctgt cgaagtgaaa acaatcccga ttgatctggt
     8581 tgtttttatt cgctgtttat ctatttttat ttctaacgca ttcgatcatc gtgtagccga
     8641 tcagagtccg attacgatca atctgctgga agaaaaagag ggatcacgtt ttagtatagc
     8701 gaacaaggca aatccaacga atttgacgct atcggagatg gtcaaaagag gaaaaacctc
     8761 taaaaaaaat ggcgggttag gtctatacag tgcgaaaaaa atgttggatg tttacgaaaa
     8821 tgcagaacta aaaatcgaat tttcaaaaca gaatcactat ttttttgtag aaatgtatct
     8881 ggcatctaga aagagcaaat cagcctactg acttactgtc aaaggataag tttgaggaaa
     8941 gtctacacgg agatagagac aacaacacag catattcaaa agaaacagaa gcgtgatgac
     9001 cgttgtcacc cttctgtttc ttttgctatg tagttaaaca aagtagttga cacttccgct
     9061 acttagtatt agtacgaaag tatacttcat gatactaagt agataagaag tgaaaagcgg
     9121 aacccacgat cgtcaaaatg ctaactgctt tgcttacacc agataaaggg aagagcaata
     9181 tcgcttattt taggcgtgga gaagcaagca aatccctacg atcacaaaaa aaatgctgac
     9241 ccagcagtta agagagttag aacaagatga aagtatccat cgaaaagcct ataaccaagt
     9301 cccgcctaaa gtagagtact cgctcaccga agaaggaaaa tcattaagag agattctagt
     9361 agcgttgtct gtttgggaag aagaacgtat cgaaaaacaa aaagcctcaa gggaagaaga
     9421 cacgctactg catacacatg gtggctacct aaattattga ttttctatga catttatttt
     9481 aaataaaaga aaacaggagt ttttttaaaa gcatcatcta ttttttatgt aaaaaaatgg
     9541 tatgattaat caagtaagaa gtgattggaa gaaggcaagc aaatgaatag tatggaaaaa
     9601 gtcaaccagt ttagagatga acgtaattgg cgacaatttc ataacgaaaa agatttggct
     9661 atctctattt ctttggaagc atctgaattg ttagaacttt ttcaatggaa aacgccggaa
     9721 gaagtaaaag aaacacaatt agaacgaatc aaagaagaac tggcagatgt attgatctat
     9781 tcatacatga tggcagacaa ccttcacctg gatctagatg agatcattga agaaaaattg
     9841 atcaaaaata atttgaaata tcctgtagaa aaaagcgcag gtgtacgaaa aaaatacaca
     9901 gagttgtaga aatgtgtcat tcggctgttg gaaagagcaa aagaaaaaca gtaaaatgag
     9961 tctggaggga gagaaaatga aaaaaaaagg aaaagcaaaa agatggctag tcaatatcct
    10021 cttgttcctc ttgttattag taggactagc tttgattttc aatgaacaga tcaaggatta
    10081 ttttgtcaga gagacaggag ataaatatgc tatagcgaat gtaacaaaag aagatctgaa
    10141 aaagaataac gataaagatg tcagttttga ctttgatgcg gtagaaccaa tgacgacaga
    10201 aggggtcatg aggtcgcaaa tgagaggaac agatctacca gtgattgcta gtatcgcagt
    10261 cccttcagtt tcaatcaatt taccggtttt caaaggcttg gataatacgt cactacttta
    10321 cggagcagga acattatctc cagatcaaga aatgggaaaa ggaaattatg ccttagcaag
    10381 ccatcgtgca acgaaccctg aattgctgtt tacaccgcta gaaaatttag agatgggtgc
    10441 caagatctat ttgacagatt tagaaaatgt gtatacatac aaaacatttt ttaaagaaaa
    10501 agttgctcct acagataccc aattactgaa tgaagtcgag ggaaaagaaa ttgtcacact
    10561 aattacttgt ggcgacatgg acgccgtcac gcgactagtt gtacaaggag aactggaaag
    10621 tgttacatcg ataaaagatg cgacagatga tatgagatct gctttcaatc tagagacaaa
    10681 aacattttag gatactggga cataagtcgt tacagattaa aaacagccaa aggatatgaa
    10741 tcgataagga aaaaccaatt tgctgaaaaa aagagaaacg caaagaggtg catctctgat
    10801 ttggttgttt tctagataga cttgtgttcc agttttttta ttttttatat gaaaaggctt
    10861 gcataaataa aaattcgatg gtatgatgat tttgaataag aaatggattg ttactgtacg
    10921 gcaggcaaaa ccagaattca gctaagaaac agtaggcgaa ttctaggttt ttttattttt
    10981 cttgagaggg cgaagcaaaa tgaaaattga taaaattttg aataacaatg tcgtcatttc
    11041 gaaaaatgga ttcggggaag aagtcgtttg tatgggaaaa ggactagctt ttcaaaaaaa
    11101 gattggggac gagatttcgc cagaagcggt ccaaaaagaa tttgttttaa gagattcttt
    11161 tgctaaaaac caattccagc agctgatggc tgatattccg gcagaggaga tcgaatgggt
    11221 caaacaagta gtcgaactgg ctgaagataa actaaaagtt gaattttcgc ctaatattta
    11281 tctgacattg accgatcatc ttcattatgc gatcacacga gcaaaagaaa atatccaact
    11341 gcctaatcca ttattatttg aaacaaaaaa attttatccg aaagaatatc aagcagcgaa
    11401 agaggctctt ttgttcgtga aacaaaagac gggtgtggaa ctttctgagg atgaagccgg
    11461 ttttattgcc tttcattttg tgaatagcca acaaggaaac gaagacatgc aagtgacgat
    11521 gaccgctacc acaatggtaa gagatatact gagtatcatc agtaaattct tcggcatcgt
    11581 gttcgatgaa gaatctttga attatcagcg tatcattact catctgcagt tttttgccca
    11641 gcgttatctc aaaggggaag gatctgacga gcaagatgaa tttctctacg cattagttca
    11701 aggaaagtat ccaaaagcgt ttcattgtgt cgaacggatc aatgagtatc ttcgacaaac
    11761 gcaaaaacag gaaatgggga tcgcagagca gatttattta acgattcata ttcaaagagt
    11821 agtaagtgag aaaaaaagca tccaatcata aggattgtta ctgtatggca ggcaaaacct
    11881 ggattgaaaa aataggttca cgaatgtttg tgactgtttt tttcaatctg ggtttttatt
    11941 ttttaaataa aaaagaaaga aggaaaaaac aatggataat caagcagttg gtcgtcgtgt
    12001 atgggaagcg gtaggaggac aaaagaatgt caaaagttta gtacattgtg ctacgcggtt
    12061 gcgattcaga ttaaaagatg aatctttagc agatacgcaa aagttaaaag aagacccgga
    12121 cgtgatccaa gtcgtacaaa gtggcggtca gtatcaagta gtgattggca gtaatgtagc
    12181 agatgtttat caatcgatcg tggatgagga aggtcttgca gtttcggagg atggaaaaga
    12241 agagtccaaa aatattttga atcgtctgat cgatattatc tctagtatat ttacaccttt
    12301 tctaggggcg atggcagccg ccggggtatt gaaaggattc ttatcattgg caacggtgat
    12361 gggctggctg acagctgata gcggtactta tcaaatattg tttgctgaag cagatggtgt
    12421 ctttaccttc ttacctgtca tgcttgcttt tacagcagct aaaaagttca aagccaacca
    12481 gtttttagca gtagcaattg ctatggcact tgtgtatcca gctatcacag cagttgctgg
    12541 tgcgggagaa gctatcagtt tctttgggat cccagtgatt ctttcaccat ctggttatac
    12601 ttcttctgtc ataccaatca ttctagccgt atgggttcaa agtaaattag agccattcgt
    12661 aaaaaaagtg attccgcaat ttctgcaaat gattttagtt ccgcttgtcg ttttagtagt
    12721 gatggttcca ttaacatttc ttgctttagg accaatcggg acggttgccg gtaatgcttt
    12781 aggcggtctc ttcaactcga tttatggatt tagtccaatc gtagcaggtc taatcatggg
    12841 aagtttatgg caagtattcg tgatgttcgg catgcatcgg ggattcgtac cgatcatgtt
    12901 tttgaatatc gaacaatatg ggtttgatgt attgatgcca atgcttcttc cagccatctt
    12961 ggcacaaggt ggggctgctt tagctgtagc attgcgaaca aaagatacga aacttcgagc
    13021 attagggatt tcttctacag tgacttcgtt atttggaatc actgaaccaa ctgtgtatgg
    13081 tgtgacatta cctttgaaga aaccattcat tgccgcttgt atttctggag gtattggtgg
    13141 agctattatc ggattttccg gagtaaaagc tttctccagc agtttggtca gcttattgac
    13201 gattccgact ttcattaata cggtggatgg tgtagagtca aatgtgacag tagcagtaat
    13261 agctacagga attgcttttg ttcttgcatt cgtaggtaca ttgattttag gatttgacga
    13321 acaaacacaa gacaatcaat tagaaaacaa acatgcgaat gcaggagaac cgattacttc
    13381 agcacggcat accttaaaaa gtcctttaac gggtaaagta ctgccattga gtgaagtacc
    13441 agaccaagtt ttttcttctg gcgtgatggg aaaaggtatc gctattgatc cagaagtagg
    13501 agaactagtt gcacctgcag acggagagat cacgactatt tttccaactg gacatgctgt
    13561 agggatcaca acgactgacg gagcggagat tttgattcat atcgggatgg atacagtaga
    13621 attaaatggg aatggctttg agatcttagt caaacaaggg gatctcgtca aagcaggaga
    13681 tttattgatt cgttttgaca tcgaagcaat tagagcagct ggctatagcg taatcactcc
    13741 agttgtcatt acgaatactg atgcatttgc cgatatactt gaattagacc aaaaagagat
    13801 tattgcaaac gaagatgttc tggcaattgt aaaataagga ggaaatcagg atgcaatcac
    13861 gtttttcaga aaaattttta tggggtggag caactgctgc caaatcaatt agaaggggct
    13921 tatgatcgag acggaaaagg actatccgtt gcagatgcta tgcctggagg taagcagcgc
    13981 tttgcgatta tcggcagtga agaatttgac tggacgattg atcaagaaaa atatatttac
    14041 ccgaatcatc gaggaatcga tcattacgat cgtttcaaag aagatcttgc cttgtttgcg
    14101 gaaatgggat tcaaatgtta tcgtttttcg attgcctgga cacgaatttt tccaaatgga
    14161 gacgaaggta ctcctaacga agcgggatta gaattttatg atcagctgat tgatgagtgc
    14221 ttaaaatatg atattgaacc tgtgatcacg atttctcatt acgaaatgcc tcttcatttg
    14281 gcaaaagaat acggtggctg gaaaaatcga aaactgatcg acttctatga acgatttgct
    14341 cagacagtat tagagagata cagcagtaaa gtcaaatatt ggatgacttt caatgaaatc
    14401 aattcggcct ttcactttcc agctttaagt caaggattag taaaaagcaa tggagcagga
    14461 gagtaccaaa atattttcca agcatggcat aatcaattcg ttgcaagtag caaagccgta
    14521 aaaatcggcc acgaattgcg ttcggatatc caaatcggct gcatgattat ctatgcaaca
    14581 acttacagca tcgatgccaa tccagtcaat caggcagcaa caatgatcca aaaccaagaa
    14641 ttcaattttt tctgcacgga tgtccaagta cgtggagaat accctgcata cacagcgcgt
    14701 acccataaaa aatatggagt cgatccggaa aaattagagc aaacggaaaa agacttccag
    14761 ttgctaaaag aatatccagt ggactacatc ggatttagtt attatatgtc tacggcaatc
    14821 aatgagactg atccacaagc agcaacttca gaagggaact tgctgggagg agtaaaaaat
    14881 ccgtttcttg aggcaagtga atggggctgg cagatcgatc cagaagggct gagaatcgca
    14941 ttgaatgaat tatacaatcg ttaccagaaa ccattattca ttgttgaaaa cggtcttgga
    15001 gccattgatc atatggaaga ggatggcacg attattgatg attatcggat cgattattta
    15061 cgtcgtcata tcgaagcgat ggctaatgca gtagcagacg gtgtagattt gatgggctat
    15121 acgccttggg gatgtatcga tcttgtcagt gcatcaacag gagaaatgag taaacgttac
    15181 ggctttatct atgtggatct agacgatgaa ggaaatggga cactgaatcg atcaaagaaa
    15241 aaatcttttg attggtataa agaagtaatt gcctcaaatg gcgtaaactt gtaattaaag
    15301 cagctgaatg aaaaagaaca cacttctcac atgtgatttg tgttcttttt ttatttttta
    15361 tgtgctattc ttttagtaat agtaaagcaa agggtgacgt tatgaaaagg acacgtgtac
    15421 tatacttgga agtcgcagat aaaattaaag aagatatttt ctcaggaaaa tatccagtcg
    15481 gttcgatgtt gccgacagaa agtgaattag aagaactgtt tcaagtcagc aaaatcactg
    15541 tccgaaaggc aatcgaactg ctggcatcag atgaatatgt cgcaaagaaa agtggtcgtg
    15601 ggacgacggt tctaagcaat cgtccttaca atcgattatc caaagcggca agctttacgc
    15661 aaatcttgga gaactcccga tatgaagtcc ggaaagaaac gctggatttg caattggtcc
    15721 atataaagaa ggaagatcct ttgttttcct ttttcggtga gcaagctgtt tgttttaagc
    15781 gcttatatta tttgaacaat cagccttata tttattttgt tcattatctt ccacaagaaa
    15841 tgatgcaatt cagcagagaa gaatttggca aagaatcttt gtatcgtttg ctgacgaaaa
    15901 aaggatatac tattgactca ttttctgatg atttctcggc agtattttta acagaagaag
    15961 aacaaggaat tctgaaaaca gacgcacaga tcggcatcaa acggatacgg aaatcgtcta
    16021 cgcaaattgg ggaaatgatt gaatattcgg aggcggtcta ccatacggag ctgcatcctt
    16081 accatattga ttatgaaaca taaataaaa
//
LOCUS       DACEUZ010000058        15818 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.058, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000058 DACEUZ010000000
VERSION     DACEUZ010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15818
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.058"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(<1..363)
                     /locus_tag="IXG44_001545"
     CDS             complement(<1..363)
                     /locus_tag="IXG44_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter solute-binding protein"
                     /protein_id="HAQ1658270.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLP"
     gene            615..1796
                     /locus_tag="IXG44_001546"
     CDS             615..1796
                     /locus_tag="IXG44_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ1658271.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            complement(1856..3484)
                     /gene="treC"
                     /locus_tag="IXG44_001547"
     CDS             complement(1856..3484)
                     /gene="treC"
                     /locus_tag="IXG44_001547"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HAQ1658272.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(3935..6646)
                     /locus_tag="IXG44_001548"
     CDS             complement(3935..6646)
                     /locus_tag="IXG44_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAQ1658273.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGTIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            complement(6656..7357)
                     /locus_tag="IXG44_001549"
     CDS             complement(6656..7357)
                     /locus_tag="IXG44_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1658274.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            7543..8142
                     /locus_tag="IXG44_001550"
     CDS             7543..8142
                     /locus_tag="IXG44_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ1658275.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            8223..9236
                     /locus_tag="IXG44_001551"
     CDS             8223..9236
                     /locus_tag="IXG44_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1658276.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            9344..10261
                     /gene="rbsK"
                     /locus_tag="IXG44_001552"
     CDS             9344..10261
                     /gene="rbsK"
                     /locus_tag="IXG44_001552"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HAQ1658277.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            10289..10684
                     /gene="rbsD"
                     /locus_tag="IXG44_001553"
     CDS             10289..10684
                     /gene="rbsD"
                     /locus_tag="IXG44_001553"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002829567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HAQ1658278.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            10684..12057
                     /locus_tag="IXG44_001554"
     CDS             10684..12057
                     /locus_tag="IXG44_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1658279.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            12414..14558
                     /locus_tag="IXG44_001555"
     CDS             12414..14558
                     /locus_tag="IXG44_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ1658280.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(14610..15651)
                     /locus_tag="IXG44_001556"
                     /pseudo
     CDS             complement(14610..15651)
                     /locus_tag="IXG44_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
BASE COUNT         4686 a         3167 c         2935 g         5030 t
ORIGIN      
        1 cggaagtaaa agcatacctt ctgtcgcttt tatcgtatgg acattgcctt cttgtaatgt
       61 tttttggatt gcggggaccc atgtttccat gttttcatca ttataaacaa agatatctgt
      121 atcttggatc tttgccatgt ctttagcaga aggttcataa tcgtgggaat cagtccctgc
      181 tggaatcaat aactctactt ctccttcatc cccgacgatt gcttttgtaa aatcatacat
      241 gggataaaag gtcgtcatca cgctcaattt ttctttactg tcctcggctt tcccacttgc
      301 cccgcaagca gttaacatac ctgctaaaac cagcatggcc aatataccta tcaatcgttt
      361 catttatgac actcctcttc tcatcttctt tcatttgaat gttccgattc gcttttcgca
      421 aaccgtaata gttacgtttt agcaacagga tttaatttat catatggtta aaagaactgt
      481 caagcgtttt cttattttaa ttgatgagaa agtttatatg aatgattgac gatatttttg
      541 caatcaacct ctatcatttt ccttttatac ccgcttttgt ctataatgaa gttatagata
      601 aaggaggaaa aagagtgaaa aaagaaacag atacaacgaa tcgtttgatc cgctttttag
      661 gaggaaaaac ctcttactat gttttaggaa tagttatctt acttgcgctg atcatttatt
      721 ttttccatca gatctcgttt atattcaagc ctgtcggaat cattatcgct actatcttgc
      781 ccccattatt gtttgcactg atcttgtatt atcttttgga accagtcatc aaacgtttga
      841 gcaagcacct ttcgaggaac ttgtcagtca ttatcgttta tgtgattatt ttatttttat
      901 tggggcttgg aagcatatgg cttattcctt ttcttgaaga acaggcaaag acattgatca
      961 gtagtttgcc agatttgttt caagattttc aagagagtct ccgacaattt ttggagaaaa
     1021 caccttttgc cgattcattt aatcaatttt tcgcttctat tgacgacgtg acgtctgata
     1081 tagctggatt tattggaaat tattgggaaa gtggcgcaca acggatcagc aacatttttt
     1141 caactgttac agctattttt attactttat ttactggtcc aattgtcgct tttttccttt
     1201 tgagagatcc tcaaaaattt tatcagtcag ttttagcgat tgtcccacca gcatttcgta
     1261 cagattttaa aaatttgaca aagattgcta atcaacaatt gggttcattt ctaaaggggc
     1321 aaatcattgc ttcatttatc cttggagcag tctactgggt ttgtttctta ctgatcggat
     1381 tagaatttgc tagtgtttta gctatttctg ctgggttgtt atgtatcatt ccatatattg
     1441 ggccgtttat cgccttcttt ccaggtttga tcattgcatt ccaagactct acttttatgg
     1501 ctgtcaaatt tgtcattgtc tggtttgccg tacaacttct ccacggtgac ttggtgattc
     1561 ctcgagtaat gggtgatcgt ttacagatcc atccgattac gatcttaatc gttttacttg
     1621 tcatgggtga tctaatggga attgtgggcg ttatttttgg tattcctatc tatacgttag
     1681 tcaaattact cgttactttt attttccgta aattcaaaca acgctacaat aaattttatg
     1741 gcgataaagg agagtatgaa aactcttcat tttctgaaga agattatttt aaataaaaca
     1801 caaaacgtcg atcttacgag aatagctcgt aggaacgacg ttttgtgttt ttgttttaat
     1861 ttttcagcaa agcaattgcc tggtatggtt ttagctccag actttgatga agaacagtat
     1921 cggaataatt atcgataaga atattggctt ctgtgaatcc ttctgggatg gcgatccttg
     1981 tatctttatc atagaaattt gttaatacaa gcagctgctg tccttcaaat tggcgaagaa
     2041 agccataaac ttccggatga tttgcttcat aagcatgata atctccttgt gcaacgattg
     2101 gatatgtttt tctcagccga atcaatttct tgtaatagga caaaatagaa tcttcagaag
     2161 atagttcttt ttctacatta atctcttcta gcttgcctcc aacttttaac caaggtttcc
     2221 ctgtagtaaa tcctgcttgt tctgtgtctg accattgcat gggtgttcga gagttatcgc
     2281 gagattttgc tttgatccga cgaaacgctt ctttttctgt gaatccttgt tcaaggagta
     2341 acttataagc attcaaactt tctacgtcaa tatagtcgga aatcgatgaa tagtctgggt
     2401 cgatcatgcc gatttcttct cccatataaa tatagggcgt cccacggttc aaatggatca
     2461 tggttgctag catctctgct ccttgttttc gatactgatc gatagctaca aatcgattga
     2521 gcgcacgtgg ctgatcatga ttattaaaaa atagagcatt ccagccgtta ccctcactca
     2581 tcttttctcc ccatgtatga aatagtcgct ttaattctgc aaaatcaaat ggaggagtag
     2641 accatttatt cccattttca taatcgactt tcaaatgatg gaaattaaag accattgata
     2701 attcttctct actaggttct gtatacaaga tacaattttc gatcgtcgta gagctcattt
     2761 caccgactgt gatgatagtg gaatccgtcc caaacgttgc ttggttcaat tcttttagat
     2821 acgtatgggt gattggtttg tccgtatatt caggtttgcc atcattttct ttattatttt
     2881 tcaattgttc gtctttaccg ataacattga taacatcgaa acggaatcct tttacccctt
     2941 tttccatcca aaaacgaaca actttgaata gttcctctct tactgcttgg ttccgccagt
     3001 tcaagtcagc ttgtgtccga tcatataagt gcaaataata tttccccgta tctccaaatg
     3061 gcgaccaagc acttccacca aattttgata cccaatccgt tggctcttct cttaaaatga
     3121 agaagtcctg ataatgctgg tcccctgcta gtgctttttt gaaccattca tgttctgtcg
     3181 aaacgtgatt cagcaccata tcgagcatga tttctatccg gtatttcttt gcttcggcta
     3241 ttagctgttc gaattcctcc atcgtaccaa agataggatc gatggctgtg taatcagaga
     3301 tatcataacc gttgtctttt tgagggcttg gataaatagg gtttagccaa atcatgtcga
     3361 tgcctagatc attcagatac gggagttttt ctttgattcc ttttaaatct ccaattccat
     3421 caccattact gtctttgtaa gatttgggat agatttggta aatgactttg tctttaaatg
     3481 tcatggatat ttacctccat tcaactgatt tattacctac cacaacttat atatacaagt
     3541 tatcatttat tattataact gaaccgaaag cgtttgtaaa atttatcttt atacattcta
     3601 agaggtctgt tgcccaataa aaaacaagag atcagacatg tatttgtcta tcctcttgtt
     3661 taaaatagca aaatattctt gtttcttcta taaatggtgt tcaaaagctt ttatctacgg
     3721 caatcagtct tctcaaatgt aaaacatgag gagctaattt tatttgagta ttctttttgc
     3781 tcgatgcagg acggcttttt tacaacctct gattcatggt gttcaatcag ctgtccttcg
     3841 gtattagctc aaaaaacggc aaaatatgaa aaactatttt gccgtttgtt tctctaatac
     3901 tcaggtcaaa ccgctgattt cacaacctct gtttttattc attcgatttc aacgcatcaa
     3961 tcatatcgat atgctttaat ttgaaatggg taacaatcat cacaataacc gtaaatacga
     4021 tagttaatcc tgctgataag acataagccc accatgtgat cactaatgga aagatgacat
     4081 tctccataga cgcttgttgc aagatgaagt cagtcaatat atatcctaca ccaaatcctc
     4141 cgatgattcc taaaagagta aagatgatat tttctcgaac aatgtacatc gtgacttctt
     4201 tatcaaaaaa tccaaggact tttattgtag ataattcgcg tacacgctcg gaaatattga
     4261 tattagttag attatacaag acgataaaag ccagcagtcc tgataatacg acaaagatta
     4321 agacaaccga gtctaagttc gccattgatt cttcttgcgt ttcgatttgt ttggacatga
     4381 aagacgtatt tgtcacagcg tctgtggcaa gcagtttctc ggaaagcatt tcttcttctt
     4441 ttgttgtcat cttatctgta cgcagcaaaa atgcattatc catcatcgat tgatcagtaa
     4501 ctgaagcata atattcaggg gacatataaa taaagctacc tagatagttt ttcacgattt
     4561 cttttacttc gactttccat tctttttgat cttcatcata aagcgaaata tgatctcctg
     4621 tagataagtc aaatagttct gctacttttt gcgtaatagc gattcctgtt ttaggcaagg
     4681 agcgtgggtt ctcttcaaga tcagttaaat ggataaaacg atccaaatca gctgttttac
     4741 ttggtacgat cactgtcaga ctttgcgaag catgtccttt ttttctcaat tctacggttt
     4801 gagtatgaat agctaggatc tcatttactt tttcttgctt ctgcagcata tccgctgctt
     4861 tttgacgact atcttccgtg gatgaatcca atgtaacgat tgcttgatag tcaatgatcg
     4921 ttccaaattg tttggctgaa acagaactta gagaatcttt caatcccacg cctgcaacca
     4981 tcagcccggc acatccagca attccgataa tcgccataag cattcttgac ttatatcgga
     5041 agagatttcg gtaactgatt ttttgattaa agctaaggcg tgaccacagc ggagtgatgt
     5101 attcaagaaa aatgcgtttc cctggcttcg gtgctttcgg ttgaagcaat gcagcaggcc
     5161 gttctcttaa atctttgaat aaaacgatca ttgcagcgcc aaatgcggca atgaagaatg
     5221 cggcagctgc ttgtaaaatc ggcggccagt cataaaaaat aagcgcactc ccaatatcat
     5281 accgctcgtt agacagctca aaaatgatcc ttggcaaaag attcgttcct aatatcgttc
     5341 ctaaaacaat acctatccct gaggctagac ttgcatagat tgcatatttt tttgcgattt
     5401 caactttcgt ataacctaac gcctttaatg tcccgatttc cctgcggttt tcttctacca
     5461 tacgtgtcat tgtcgtaaag gtgatcaatg cggcaataaa gaaaaagaaa acaggaaaaa
     5521 cattggcaat cgcagcgata cgatcagaca gatcaccaaa ttcttgaaaa ccgacatttt
     5581 ctgaacgttc attgtatagg tatgatggtt tagttaaatt ttcaattttt tctgcatttc
     5641 gattcaattc ttcctctgca gaggccagtt cacgttcttt ctcaactaat tgcctttctt
     5701 gttcagcaag ttgagcttct tgttgagata atgtttgaac agcgattgtt cgttgcgctt
     5761 caggaagctg ctggatagtt gctgactgtt gcgctaactg ttttttagca tcttctaacc
     5821 gtgttcttgc ttgcgttgtt tgtgctttgc catccgctat tttttgctgt gccggttcta
     5881 attcttcgtt ggccgatttt ttcagctctt ctaagcgttc ttcgggtcga tcttgtaaat
     5941 acttttctgc cttttcttga ttttgctcca ttttatcttt gtaagctttg ctgtacgtat
     6001 tcaaattctt tacgtttgta aaatcaagat ataagacaga ataaatatct gttttaaaaa
     6061 cggattcagg taaatagaca aaataatcga cgctaccatt tccgacgttc gcgtatcctc
     6121 tctccaaatt gttgatgaac aatggagaac ggacaaagcc cgtgatggta tactcttttt
     6181 ctttcaattc atctgaatca atctgataaa tatcaccgat tttataccct tcatcttccg
     6241 ctttttcatc caatacaagc tggtttggtt tttccgggag ttttccatca gttaattgta
     6301 gcttgttctg cttgttattt ttgttataag aaaatatttg aatcacttcg tttttgccag
     6361 tattggaata atagatctgt tttcctgttt ctacttttgc atctgggatc ttttctgctt
     6421 gagcaatatc tttttgtgtc aagccaccag tagaaacgat ttgcaaatca gaaagtcctg
     6481 ctgtaccaac atagtgatcc atcgtttttt gcatgacagg tccagcagtt ttgacaccta
     6541 caaataaaag tgttcctagc atgacaataa ggatgattgc aatgaatcgt cctttagatt
     6601 gccgaatttc gcgaaaacct gctttaagat atgttcttgt tttcataaaa tccccctacc
     6661 atacaatctc atcaatggat gaaggatgat catttaattc aacagacctt actttagcat
     6721 cattaatgtg aatgactcta tcagcgatgg gcgctaaagc agaattatgt gtaatgatca
     6781 aaacggtatt gccgtgttgt ctgctagcgt tttgcaataa tttcaatact tgcttgcccg
     6841 tttcaaaatc cagcgcgcct gtcggttcat cacataaaag aagtttggga tttttagcta
     6901 gtgcacgagc aattgaaacc ctctgctgtt cgcctcctga taattgggac ggaaagttat
     6961 tcaatcgatg agctaggccc acttgcctca atacctctac aggatcaagc gcatcaggag
     7021 aaacttccgt tgccaattcc acattttctt ttgctgttag attaggaacg agattgtaga
     7081 attggaaaac gaaccctaca tctgttcgtc ggtaagcagt aagctgttta tctgaaaatt
     7141 gcgcaatatc tgtgtcatca ataatgatct gaccttcatc aggtgtatcc atcccaccta
     7201 aaatattaag aatcgttgac tttcctgctc cacttggacc aagtataatc actaattcgc
     7261 ctttttcaat tgaaaaagaa atatcgtcat ttgctgtaat ggtcgtttct cccattttgt
     7321 atcgtttgta ttcatttttt acttcgacat acttcactct gattcctcca ttttcacgat
     7381 atttctcgct atatctatgc atcgaatgcg ttttctcaca cttatttatt ttaccactga
     7441 atttctctag actaaagaaa ggaatccttt aatttagttg acaaataaac gatttatgat
     7501 ttacttacat aaaaaacatt gttatttgaa agaaggaaca tcatgaatga tttaaaggaa
     7561 aatatgattt ttgatttatc aaaaactgct tttgtcgcga tcgacatgca aaaagagatc
     7621 gtaaataacg gtacactctc tccacacact gcagcatcta ttctgacaaa caatgatttg
     7681 ttagtaagaa cattgaaacg taccgctgcc ttgaaagttt tagtgaacgt agacatctcc
     7741 actttccctt atttgagtca acaaactgat atgggtggaa tgactgcttc tgtacctcca
     7801 gaattcactg atttactttt gaaggattct ttgaaagata cagataatat gttgactatc
     7861 acaaaataca atccaagtgc tttttttggt acttctcttg atcttcaact tcgccggcgt
     7921 ggaatcgaaa ctatcatttt aagcggtgta gcgacaacaa acggtgtata tgctactgca
     7981 ttagatgcct ttcaacacgg gtaccatatc gtgcttgctg aagatgcttg cagtgatcgt
     8041 gacaaagaat cccatcaatt gttcataaaa aagatatttc cgaaaactgc acgagtacgc
     8101 tcaacgaaac aaataatcga agctatccaa caaagcaaat aagtactggc acatgtgatt
     8161 ttcatttact tctttttgat ttcatgggaa aataaccaca ggatttagat aggagtatga
     8221 acatggtgaa aaaaaagatc acaatcaaag aagtcgctaa actttccggc gtttcaatca
     8281 caactgtctc cttgatatta aatggaaata cggagaaatt cagtccgaaa actgttcaga
     8341 aagttttagc tgccaaagaa gaattaggct atcagcctga ctattttgct agacaaatga
     8401 ttaccaagga aacgaaaaca attggtgtac tcgttcctga tatcacgaat ccattcttca
     8461 gtacactgat gcgaggaatt gaggatatct tgtataagca gaatttcgtc acgatattat
     8521 gtaatgctga ttctgatcat caaaaagaaa tcgaatatct agccgagctc acacgtcgag
     8581 gtgtggatgg attcattatc gcgacttctg ctgtctctac agatgcaatc aacgaaaatt
     8641 tgaaaaaaca aggccggccc ttcattgttt tggatcagaa aaaatctgaa ggatttagtg
     8701 atgccgtacg aacagacgat tttcgcggtg gttacctagc gggcatgcac ttgcttagtc
     8761 taggacatca aacgatcgcc cttgtctatc ctgaaaatcc accggaaaat gtccacgctc
     8821 ggattgaggg gtttaaatct gctcttgatg tttaccaaat ccctcacgac caactgatcc
     8881 tcttacctac acagttctca aaacaaggtg gctaccagat cacagcagaa cttcttgatt
     8941 ctgctgcaac tggtgtgttt gctttaaatg atgaattagc ttttggatta tatcgcggat
     9001 tagaggaagc tggaaaaagc atccctgaag actacagcat tattggctat gataatatag
     9061 atatgtgcga gtatatcaag ccgaaactga caacgatcgc acaaccaatc ttcgaattgg
     9121 gacaaacatc tgccaagtta ttgttagatc ggatccaatt cccagaaaaa gaatgggaag
     9181 aaaaaagatt acctgtacga ttagaaaaaa gattttcaac tgcacccttg aaatagcttt
     9241 caaggtgtgc tatattttta tcgaactatt aaaacgtttt actaaaacgt tttaaaaaat
     9301 aattttccca tcaaaataaa tgagatagtt ggaggtttgt aaaatgaaca cggttacagt
     9361 aattggaagt attaatatgg acactaccct tcgtttaacc aacatgccca agccaggcga
     9421 aacgatgcat gcgcatgaga tttttcacgc tggcggaggt aaaggtgcca accaagcagt
     9481 ggcagccaaa cgttcaggcg cacggacatc ttttattgga ggcgttgggg ctgacagcga
     9541 aggacaacag ttattagatc tactgacaaa agaaaacatc gatacatctg ggatagctga
     9601 aatccaaggc gccacaaccg gacaagcaat gattatggta gacgcagcag gagaaaatag
     9661 tatcttgatc catgcagggg caaataatgc tttccatgaa caagaagtcc taaaaaacaa
     9721 gcaactgatt acaaacagtg actttattat tgctcagttt gaaagtagct tagatgcgac
     9781 gatcctagct ttttctattg caaaagacgc aggaaaaaca acgatcctga atcctgcacc
     9841 agctcgtgaa acgattccta ctgaactact tgaaaagaca gatatcatta taccaaatga
     9901 aacagaaaca gaaatcatta caggaatcag agtgacagat cataacagtt tggtcgcagc
     9961 agccgaaaaa ctccatgaat taggtattgg aacagtaatc attacactag gcagtgccgg
    10021 cgctttctat catacagaaa aagaacacgg tatcgtccct gcttttaaag tggatgccgt
    10081 tgacacaaca gccgcaggtg atacatttat tggcgcatta agcagcacac ttcagccaga
    10141 ccttagtaac ttgaaagaag cgatccttta tggaaatcta gcctcttctg ttgctgtgca
    10201 gtcttatggt gcgcaacctt ctatccctta ccgcgaagct ttagaacaat cagcagtcta
    10261 aaattaacaa tgagaggaag aaacaaacat gaaaaaagga aaagtaatca attcagatat
    10321 ttcacgagta atcgcacaaa tggggcactt tgataaatta agtatcgggg atgcaggcat
    10381 gcctgtcccc tttggtactg aaaaaataga tttggctgtc tcaaacggtc ttccaagttt
    10441 tttatctgtt ttagaaaacg ttttagaaga acttgaggta caatcgattg aattggcgga
    10501 agaaataaag acaatgaacc cggaaatatt ggaaaaaatc aaattattgt taccagatac
    10561 accgattagc tttatcccac atgaagaaat gaaaaaagag ttgaataact gtcatgcatt
    10621 tattcgtaca ggcgaaatga caccttacgc caatattata ttagtgagtg gagtggtctt
    10681 ctaatggaaa cgacaatgaa tcctgaacag atggtactgt ccgagcagat tcatatgctg
    10741 attgaacagt tgggaggaaa agacaatatt gattctgtcc ttccatgtgc gactcggctt
    10801 cgtatcaaat tgaaaaaaca gccagtagcc aatgcagaaa aattaaaaca gcaaatccct
    10861 tccattaagt atttcatttt actcgataga tatgcagaaa taatcatgga tcagggattg
    10921 gctcctgctt acaaggaatt gacgcgtcaa atcgatcatt cagctccgaa agaaaagcta
    10981 aatccgatcg aaaaactatt tggtttgata tcggaaatct ttacgccgat cattccgctg
    11041 tttgccggtt ctggtatatt aaaaggtctg gttgttttag cgactacgat tggctggctt
    11101 agtgaaacaa gcggaactta tcatattttg tctgctgcgt cgaatgccgt cttttatttt
    11161 ctgccaatct ttcttgcttt ttctgctgcc aaaacgttta aagtgaatgg atatattgcg
    11221 gctgtcattg ctgcagcact gattgaaccg aatctgacct ctttactttc agacagtggc
    11281 aaagcagcta ctgattttct ggggattcct gtagtactga tgcagtatac gtctactgtc
    11341 atgccggcga ttttaggtat tttcttttat tcttttgttg aaaaattcct caaaaaatat
    11401 attcgcgaaa atatgcaatt agtatttgta cctttacttt cattagttat cacggttcct
    11461 ttgacgttaa tggtattcgg gcctgcgggt gtttaccttg gcgaaggttt agctgccgca
    11521 attacttgga tgatggatat taacggaccg atcagtggcg caatcatcgc tggtggttgg
    11581 aatattttgg taatatttgg tatccaatgg gctgtaaacc ctgtcatgat cagtaatatc
    11641 tctgcttatg ggttcgaccg aatcgttccg ttgacaggag cagccaattt tggaatggct
    11701 ggtgcagctt taggtgtatt tctgcgaagt aaacgttcaa aaacccgttc gatctccggt
    11761 tctgcttttg cttcgatttt acttgcggga gtgaccgaac caactgttta cggaattgcg
    11821 atcccattga aaaaaccatt tgttgctgct tgcattggtg cagctgctgg tggagctgta
    11881 atgggatttg cccaagtgaa agcgatcgct tttgtatttg gtagtctgac cacattgcct
    11941 gcattcattt ccggtacttt tttctggtac ttagcaggac tggctgttag tctagtcgta
    12001 gcaatgatca ctactctcgt tagcgggttt gatgaagacc taatgtctta tgaatagtta
    12061 atgaaaaaga ctgaagtatg agatgtttat cacatatttc agtctttttc tttttaggat
    12121 cattattttt ataaattttt gtgatacttt ccaaactttg gtataattta catatgcata
    12181 accattttta tccccacttg tttatcccca aacaaaggtg gtctatgcaa taaaatgaac
    12241 gtcaaagaaa aacgaatgaa tgctcgttgc ttatttacaa attgttttat ttagaataca
    12301 cagacagatt tatgaaaaga aaaagctttt cgacacgatt atctgttcca aaaaatgtct
    12361 aatgaatgct gaataagaca gaaaatcaat gaaaggaaaa ggactagttt attatgaaaa
    12421 ataaaaaaac gatacgtttg ctagctgcta cttggctgat tgctccgtct gttttgtcga
    12481 ccaataccgt atttgccgta gataatcaaa tagctgatca aacagtcaca acagatacga
    12541 ctacatccag ctcagctact agtgaaacaa cggattcttt tacagaagac ccaactggaa
    12601 caactaactc aacaacagga tcgaccgagc caactgaaat tgatccggct gatccatcag
    12661 aaaatgaaaa gccagccgag acatattccc ttacaattgc tccttctttg attggtaaga
    12721 tccaactgac tgcttcgaca gcggattccc ctgaacagca acctttggat attacggaaa
    12781 gtggaacggt agaaggttta ttagcggaaa cgaaggtaac ttacactatt acgccagctg
    12841 aaggtgaaca aattgcttca gtcgctgtta atggtgccaa agaagtaaat tatgatgcaa
    12901 agacctttac agtaggtacg aacaatttat ctattactgc agaatttaaa gctggaccgg
    12961 atacttcaac agagaaacca actgatccta gtgaagaagt ccctagtaca ccagatccgg
    13021 aaacgccaaa caaaccagat gtgggtaata acaacaataa taatggttca aataattcaa
    13081 acaataataa caataattcg aacagcggga attcttcgaa tcaaacaaac ccatctggat
    13141 cgaataacaa tggaaatact cagagagaac caaaccatcc aaacaatggt tcatcgaatg
    13201 gcggaacgaa caatgcttca tctcgtccaa atacagcaaa tcaaagcggt atagagaatc
    13261 catcttctgc ttcttctgat tttgttgtaa aatcaccaat tgatgctgta ttgcctacaa
    13321 atacaacgaa tgtccaacaa gcaatcgttc gagaagcatt caagcatctt ggaaaaccat
    13381 atgtatgggg agcaaaaggt ccaaatactt ttgactgttc tggtttgact tattacgtat
    13441 atatgaaagc aaccggtcat tatatcggtg gctggacagg cgaacagcaa tatgcaggta
    13501 cgcagattcc agtaagtcag gcccaacctg gcgacctcgt cttctgggga ccttcttccg
    13561 gtgttactca ccatgtaggt atctatattg ggaacggaca atttatccat gcgcctcaac
    13621 caggagataa agttcgagta acttctattt cagatttcac gcctgatttt gctgtccgag
    13681 taaacttagc aggtttacct gctgcttcag gttcgctgac aggcggctca tctattttag
    13741 atggattaga tggctcgttc catttcagtc aaaaccaaac aactgatcag ttcttgaaaa
    13801 agattgcaga tgatgcacag gaaatcgggc aaaaagaagg aatctacgca tcagtcatga
    13861 tggctcaagc tatccttgaa agtggatctg gaaacagctt attatcaagc gaaccaaatc
    13921 ataacctttt tggtatcaaa ggatcatata aaggaagcag cgtgaccttc aatacacttg
    13981 aacaagatag ttccggacaa agctaccaaa tccgcgcaca attccgtaaa tatccttctt
    14041 acaaggaaag tttggaagac tatgccgact tgatcaaaaa tggactgact ggcaatcctg
    14101 atttctacaa accaacttgg aagtcggaaa caaaagacta caaagaagca accaaatatt
    14161 tggaaggacg ttatgctaca gatcgccaat attcacaaaa attgaacgcg atcatcgaag
    14221 cgtacgacct gacaaaatat gatgaaccga aaaaagaagc agcaacaact gaagaagcaa
    14281 acgctgaaat cctcaatatg tctaagcgat tcgatgtgcc aatcaaatgg acaacagatg
    14341 gcgtgccaat cacaggatta gaattttcaa cgataaaacg cgtcaacaac gcttcttcca
    14401 tcttccgtgt cgcttcaatc tgggatttgt ggaacaattt tacggcaaga cagataccag
    14461 aaacaactac tcgtacagtt aaagctgcaa ctgttcctgt actaaccttg ttaagtttag
    14521 acaaatcatt ctggcttgat ctgcctttaa agaaatagaa taaattaaaa aggggatgtg
    14581 acactgtcac actccctttt taatttattc tatttcttta tatttatttt gtattccgtt
    14641 acaaactcgc gtacttggtt aggttccaga gtgacatctc caaaactttc aagtcgctcc
    14701 gctcctggca gttcttgagt ctcaaatgtg ataccgccat gatttactaa tttattcccg
    14761 cgcatttccg gtccatcttc tccaaaattt gctgtaaaaa tcacgattcc tgaagcatct
    14821 gtttttactt ggatcgatac tttttcatct gggcttgtca attccgcagc atttagtaaa
    14881 ccagattgtt ttttcaacaa aaatggatga tcgatgccgc ccactatcat tgattggcga
    14941 aaatcactgg caaagacctc acctaatttt ttaggacgtt ggaagtcaaa tgctgtgcca
    15001 cttactgaaa ctacttctcc tgtaggcgtt gtatctttat tgagcgtagc aaataatcgg
    15061 ctattcaacc aaagtatatg ttcgtcgatt gactgtgctg gatcaccggt taaattgaaa
    15121 tagacatgat ttgtaggatt gaatactgtt ttgcggtcac tgatcccttg gaccgtcact
    15181 cgccatgtat catcgttcgt cagcgtatac cgtacttcta catctaaagt tccaggaaat
    15241 ccgttttcca tatctgggct agtctgatag aaaatgaccg atgcttcgtt ttcaccattg
    15301 atgactgtat aatgccactg cttggcttca aagctaggtg caccaccatg taaaccaatg
    15361 accagtgtcc gggtctgttt ttacttcgaa cgtttcatca tctaatttga atgtgccatt
    15421 cgtaatccgt ccagccatac gtccgattga agcaccgata taagggtctt tttctagata
    15481 ttcttttgct tcatcaaaac ctaagatcaa ctctcgtttt tcattatcga ttattgtttg
    15541 aaaatgaaca acccgtgccc caagatcact gacagacaaa gatgtcccat tttgattcgt
    15601 gatcgtaatc aagtgatagt tttcgccaaa attttgttca ttgatttcca tgttccttgc
    15661 accctctttt ctttcatttg cactgattcc agcatacatg gaaaccgttt tcttaacagc
    15721 ttattccgtg agtaacgaga aaaaatttac tatattctaa taaaagaccg tgaaaaaaag
    15781 gttgtgacaa aaatattgtc acaacctctt tttccgaa
//
LOCUS       DACEUZ010000059        15728 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.059, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000059 DACEUZ010000000
VERSION     DACEUZ010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15728
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.059"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(<1..263)
                     /locus_tag="IXG44_001557"
     CDS             complement(<1..263)
                     /locus_tag="IXG44_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1658281.1"
                     /translation="MLMEGKTQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINL
                     YYSNKNYFKIPATKTRMKKDVYFLFDIVTDVPDARSDHRRYDY"
     gene            complement(483..849)
                     /gene="ssrA"
                     /locus_tag="IXG44_001558"
     tmRNA           complement(483..849)
                     /gene="ssrA"
                     /locus_tag="IXG44_001558"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(1020..1793)
                     /locus_tag="IXG44_001559"
     CDS             complement(1020..1793)
                     /locus_tag="IXG44_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ1658282.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            1937..2287
                     /locus_tag="IXG44_001560"
     CDS             1937..2287
                     /locus_tag="IXG44_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HAQ1658283.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            2268..2984
                     /locus_tag="IXG44_001561"
     CDS             2268..2984
                     /locus_tag="IXG44_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1658284.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            2984..4432
                     /locus_tag="IXG44_001562"
     CDS             2984..4432
                     /locus_tag="IXG44_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ1658285.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4502..5011)
                     /locus_tag="IXG44_001563"
     CDS             complement(4502..5011)
                     /locus_tag="IXG44_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1658286.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(5001..5726)
                     /locus_tag="IXG44_001564"
     CDS             complement(5001..5726)
                     /locus_tag="IXG44_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1658287.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWHLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            complement(5949..8069)
                     /locus_tag="IXG44_001565"
     CDS             complement(5949..8069)
                     /locus_tag="IXG44_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAQ1658288.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYISAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            8299..9477
                     /locus_tag="IXG44_001566"
     CDS             8299..9477
                     /locus_tag="IXG44_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HAQ1658289.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            9493..10251
                     /gene="aroD"
                     /locus_tag="IXG44_001567"
     CDS             9493..10251
                     /gene="aroD"
                     /locus_tag="IXG44_001567"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HAQ1658290.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            10274..10759
                     /gene="ybaK"
                     /locus_tag="IXG44_001568"
     CDS             10274..10759
                     /gene="ybaK"
                     /locus_tag="IXG44_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HAQ1658291.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(10830..12083)
                     /locus_tag="IXG44_001569"
     CDS             complement(10830..12083)
                     /locus_tag="IXG44_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ1658292.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            12200..13549
                     /gene="gdhA"
                     /locus_tag="IXG44_001570"
     CDS             12200..13549
                     /gene="gdhA"
                     /locus_tag="IXG44_001570"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HAQ1658293.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            13661..15007
                     /locus_tag="IXG44_001571"
     CDS             13661..15007
                     /locus_tag="IXG44_001571"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HAQ1658294.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(15315..15701)
                     /locus_tag="IXG44_001572"
     CDS             complement(15315..15701)
                     /locus_tag="IXG44_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ1658295.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         5148 a         2811 c         3165 g         4604 t
ORIGIN      
        1 aaatagtcat aacgccgatg atcgctccgt gcgtctggca catcagtcac gatatcaaac
       61 aaaaaataca catctttctt cattctggtt tttgttgccg gaattttaaa gtagttctta
      121 ttagagtaat agagattgat taataagcta tcttcgattg ctaaaaagaa aacttctgaa
      181 tcccacacct tataaaaatc tttgacaaat ctattcgaag gatcaaattt aaaccataat
      241 tgtgtttttc cttccatcaa cataatgttc acctcaaaaa gagtatacga actaatgttc
      301 tttttgtaaa gtaggaatat cattgataat atggatggaa catatatatt aaaaagtgtt
      361 ctttttgaat gctattttga atgccatttt cattcattta acgaaaatct gcgaaaataa
      421 aaaatgctca aacccttata aaataaggat ttaagcatct agcgaaatct gctgaaatct
      481 cacaatggag acggcgggag tcgaacccgc gtccaaacac atcgccactt aaaaatctac
      541 gttcatagtc aactcattta aggattcact ttacagcttg ccgagtgaca ggcattctgt
      601 atcgctagtc tgatgttctc tttttcaact tacagacgga aagttgaaac gtatcccact
      661 aaagttagga cccttactcg agcacatggg cgatgccgag aggatctacg ctaactgttt
      721 ttaggcagct aaagcgtaag agttgttttc gtttttagca gttatattta actgtaacgt
      781 ttttacgtag acgtaaccta cgaaacgcaa ttcaagctcg actgtgcctg tcgaatccgt
      841 aacgtccccg ttagctaaca agggttacgt gtatttttca tacaaaatta gtatagcata
      901 tattgggata aattcaataa aaaagcgaat catatcccat tatttatata ataactgccc
      961 ctttcttaag aaaaaataag cgattttctt aagaaaagag gtagcttcaa aaaaagatct
     1021 taaatcagat caaaatactt caatgcgtgg acaattccgc cttctgtatt tttctttgtg
     1081 ataaaggtag aaagttcttt taatccgtca taggcgttgc ccatagcgat tttgttgtcg
     1141 caagcttcca acaaggcaaa atcattcgga ccatcgccaa atccaaaagt aggaactttt
     1201 tccagctgaa gttctttttg cagacgtttg acacctgccc cttttgagat tcctttcttc
     1261 actgtatcga tcgagtaagg accattccga taaaaagtta gctcaggaaa atgttcgtga
     1321 tagtattcgt cacctgattg agataagatc agcaacatat ttactgtgtt ttctttaaag
     1381 cctaaaggat caatttccgg aacatctgaa tgaatgaagt cgtaagcatt gatcattgtt
     1441 tggctatgac cggtacacca gattttctga tcgttatagt aagaaagttc atgaccgttc
     1501 aactgtacat gatcatacat tttttgacat tcttctgaag aaaatgtttg agaatagatt
     1561 tcttcgccgt ctactcgaat gaaagaacca ttcatggtga ccgcagaagt aatgcccgca
     1621 tcttccatga tagagtggat ttctgcttct gtacgtcctg tagcgatgac ggggagaaca
     1681 ttgttttctt ttaatgcgtt catagctttt gccacttcgg gtgtgatttt tgatttcgca
     1741 tctagtaatg ttccatcaag atcaaagaaa gtgattgcac gataatttga cacaaataaa
     1801 actccttttt tattggaata agtttatctt aacaaagaat gaacaaaaga caaacctttc
     1861 gtgaagaatt cctgaacata ttggttcagt caacaattta gagtatacta taagaagaga
     1921 atttaggagg gaaagaatga tttttttcag tattggtatc ttattcttaa taattggcag
     1981 tctttttcgg attctgccct caaaaggaaa tctgcctttt tatgggtatc attcgcctct
     2041 tgccgcaaaa acagacgctc attggcggct ggctcaaaag acaagcggaa actggttttt
     2101 tcttatgggc ttactgatgg cattgatcgg ctattattta aagacatctg ggcatacgaa
     2161 ttattttctg attgaaatgc ttctgctagt atttccaatt atgcctattt ttattatgac
     2221 agaaaagaaa ttacagaaat atgacctgga aactggaggg aacgataatg aatattttaa
     2281 tgattgaaga taatcaatct gtttctgaaa tgatgcagat gtttttctta aatgaaggtt
     2341 gggaagcaac tttcaagtat gatggaaaag aagggctcga tgccttttta gaaaacccac
     2401 aacattggga tatgattaca ctagacctga atttgcccac actggacggt atgctagtag
     2461 gaagagagat ccgcaaagtt tcaagtactg tgccgattat catgctgaca gctagagatt
     2521 ctgaaagcga tcaggtgatc ggcttagaga tgggggcaga tgattatgtc acaaaacctt
     2581 tcagtcctct gacgcttatt gctcgaatga aagctttgca tcgccgtagt gagttggcag
     2641 aagcaagaga agaagcagta gtttcagatg atcattttga tatcgagact agtcatttca
     2701 agatgaatac gaaaacacga gaagcgtatc tggacaaccg gccaattgaa ggactgacgc
     2761 ctaaagagtt cgatctattg tttactttag caaaaaaacc acgtcaagta ttctctcggg
     2821 agcagttgct tgaattagtc tgggattatc agtactttgg tgatgaacgg acagtggacg
     2881 cccatatcaa gaaattacga caaaaaatcg aaaaggtcgg accacaagtc atccaaactg
     2941 tttggggagt cggctacaaa ttcgatgatt ctggtgttgc ctaatgcgtt atttgtatca
     3001 gcaactctta gctttctgcg gaatggtcgc gttgatctta ttgatcgttg gagtttcttt
     3061 tacacaactg acaaaacaaa cgattgaaga aaacaattac cagcaactgt ttggatatgc
     3121 tgaatcagtt gaaaagacaa cgcaaaccta tgccgatacg ctcccgcagt ttagtcaaga
     3181 tcaggcgttt caaaatgcat tgctgctgac agagcaagta ctgacagaac aaaatgtgaa
     3241 ctttatattt atcgataaaa acgaacgtgt catttatccg actaatacag ctttactgaa
     3301 ttttacctta acagctgatc aatggaaaaa tctgaggaat ggacgtcaag aaaaattcac
     3361 atcgaataaa aatattttag gacaagcaca ggcgacctct tacgcacttg tgccatttaa
     3421 tttgaatcat gaattctatg gagcacttgt agttagtcaa cctgcgcgaa atatcgataa
     3481 cagcgttcgt ccaattacac tgaacctgtt caaaggattt attttttcca gtatcgttgc
     3541 agttattgcc agctatgctt ttgcagcatt ccaagttaag cgtatcaatc ggttaaggaa
     3601 tgcgacgaaa gaagtgacga atggtaattt tgatgtacag ctccccgtac atgacaaaga
     3661 tgaatttgat gagttagcag atgatttcaa taaaatgacg aattcattga aagaatctca
     3721 agcagaaatt gaagaacaag aaaatcgtcg aagacaattt atggcagatg cttctcacga
     3781 gatgagaaca cctcttacga caatcaacgg acttctggaa gggttagaat acaatgcaat
     3841 ccctgaaaat cagcgagaaa acgcgattaa attgatgaaa aatgaaacag agcgtctgat
     3901 ccgtttggtg aatgaaaact tggattatga aaaaatccgt acgaatcaaa tcagtatggt
     3961 cattaaaaaa ttcaatggaa cagaaacatt gaaaaatatt gttgcacagt tagaagctaa
     4021 agcagaagca gcaggagata cattgacatt gaaagccgtt gatgatattg atgtttacgc
     4081 tgattatgac cgatttgtcc aaatcatggt caatattatt caaaacgcga tacagtttac
     4141 tgaaaacggc caaatcacga ttactttaga aaaaggctat cttgaaacga tcatcacgat
     4201 tgaagatacg ggaattggaa tgtctgaaca acaaatgaaa agtatctggg atcgttatta
     4261 taaagtagat ccttcaagaa aaaatacaaa atatggtgaa tcaggattag gtttaccaat
     4321 cgtgcagcaa cttgtacgct tgcataaagg aaaattagaa gtagaaagcg aactaggaaa
     4381 aggaacaaaa ttcgctgttc gtttgcctga cgtagaaatc aaaaaagagt aaaaaacaag
     4441 cggtataaaa gatcatgaat cgatcttcta taccgcttgt ttttactata aaatgattga
     4501 tttagcccgc gggctctaaa cgatcaatat aaggactttg agcaatagta gcatcatgag
     4561 taaagagtaa gataccttga ttttcttcat gagctaaata agaaagcaag tggagtagct
     4621 cttggatctc caatacacta agttcatcaa atgtatcttc aataacaata ttgtttcgct
     4681 tggaaagcaa ggcatgaagt aattgcagct ttactttttc aaaagacgtc aactcagctg
     4741 ccgcacaatt aagcaaggaa ggagagatat caagtttttc ttgataggcc aaacgatctt
     4801 gtttgatttc tttttccggt acatccaaca ggacatggtc tttaagattc agataaggaa
     4861 acatcggcca atttttttgg atgatcgcaa aattatccag ttttgcaaca agaacctctt
     4921 ttttcaagtg ctccaatatt ttatgctgtt tttcgagagg acacaaaata acggtcaagt
     4981 gttcagcatg aatcaaatat tcaggtttca ttacgtccag gcctcctact ttgcaaatga
     5041 tggataaaaa aagtgaaagg gaatagacta agaatgagac agcatccact gagcccaata
     5101 agagtttggg caaatccctt aagtatatct gtgaaaaaaa gatcattttg gaaatcgatg
     5161 gtaaaaaaag tctgttgatc catcggaatt gtaagtccgc ttaccgaagt caagatatca
     5221 cggatatctg gcagttcagt agtctttaag acaagaaaat tgatcctttg aagaagcgct
     5281 tcataaactg actggaagct tacgatccac aataaaacca agatacaaca aagaaagaga
     5341 ggaaagaaca gctgccagaa aataaaaagg aataatttgg ttttagaaag accagtcagg
     5401 tgccaattga tcaattcctg cttgttttgc ctgactgaat agtaaaagaa aaagataaaa
     5461 aggaaggcaa atagaaaaaa taaactgaat atgcctttta tataaaatcg gctagctaat
     5521 tctaaagccg gaagaagctc tgtagatgca gaagcgccaa taaatgaaag ccgatcttgg
     5581 acttgattgg ctaaagccga ttgcaaatgg taaagagtca ggcatgctat caataaaaaa
     5641 gcagaaaagc ttgaaaataa acaatagagt attgtctgct ttttgtaata aattaatcga
     5701 gcagtagcat agcggattat tttcatactt ttcacctcta taaaaagtat agaaaatgaa
     5761 tatgaataaa gtatgaaatt cctttagtga aatcacgaat taaaaccaga aaatattttg
     5821 taaaaagcaa gaggttgtga agaagctgcc ctattctatt accgtagatg caagcttttg
     5881 aacactgttt attcgaaaag aggagcagcg acaataattt tgtcgctgct ccttttaaat
     5941 ttattttatc attcggaaga acttgtggtc gttccgtgaa gttcttggta cgtttgcgtt
     6001 ttataaagat caacagttga ttgtccgttg tgttcactaa agacactagt tgacttgtca
     6061 ccaagagatt tttcagcttc ttttaatttc tctaagccat tcttgtagtc ataatcactg
     6121 gtatcaattg ggtctaatcc actattcgta tagaaccgaa gaagatcacc attattgatt
     6181 tggtcagaaa cagctaactg tttatcaact ttttctttca gtgaatcaat ttcattctgt
     6241 acttcctcag taggttcagt aatagcttca ccagtttcgt tactatagat cgtaccaccg
     6301 tagtaagtat attttggtgt aacaaaatca ccatcacgga aagaaacaag ctgttcatga
     6361 tctttcgaga atagatcttg tcctaactga atataatttt ttgtatctac acctagtaag
     6421 tgaagtaatg taggcaaggc atcaacttgt ccgccgtaag tgtgattgat tccgccctgt
     6481 gtttgtccag ggatatggat catataaggg acacgctgca tttgtgcatt gtcgtagttt
     6541 gtccaagtag aacttgtttt tccaactaat tcagctagat ttttatttct tgaattcgag
     6601 acaccgtaat ggtcaccgta aaggacgatg actgaattat catacaagcc gctttctttc
     6661 aaataattga agaattcttg tacggcggta tctaaataat tggctgtcgc aaagtaaccg
     6721 ttgatcgttt catcatttgt attggctaac gggaatcctg cttcgtcatt ggtgaatttt
     6781 gaataaggat aatggttaga tacagcaatg aattttgaat aaaatggttg ttgcagacgt
     6841 tccaagtatt gtacggattg ttggaaaaat ggtttatcat gcaacccgta ttggaacgaa
     6901 ttgtcatcat taacatcata ataagaagaa tcaaagaaat aatcatatcc taaattcttg
     6961 tatgtctcgt ttcgattcca gaatgtacca gcgttcccat ggaataccgc actaatgtaa
     7021 ccttgtgttt gacttaaaat atcaggtgca gcttggaaag tattttttcc gcctaattgt
     7081 gtgaacaagg agccttgatt caagccaaac aatgaatttt ccatcagcgt ttccgcatcg
     7141 cttgtttttc ctgcttttac ttgatggaaa aagttgtcaa aactgaacgt gctgttgctg
     7201 tggtaaagac tattgatgaa aggtgtaact tcatgctgga gaccattctc atctgtcaat
     7261 ttataatcga tcaggaattg ctggaagctt tctaaatgga tataaatcac attttttcct
     7321 ctagcgagcc cgaacaaatc gtcatttggt gctgcataat gttcttttac ataattttct
     7381 acttctttca tatcattggg acttgcttct gcccgcactt ggtttgtctg gtaagtttga
     7441 acaccgtcat aaaccatgaa agcattcaag ccaagatatt tgaccaaata atcacgagag
     7501 aatgttcgag tcagcaattc tggacgatca gcttccgcca taaaaagatt tcctgagaaa
     7561 atcataactg ctaatgtaga gatcgcaaaa gccattcttg ctcggatcgg gcgagggtcc
     7621 aacttgattt tttttgcaat cagcagaaca gtcaagataa taaaatcgat ccaatagatc
     7681 acatcatatg ggcgaaacag tcgaatggca ctttctccca gaccactcgc tactttccca
     7741 gcacctaaaa tcgtgttgat cgtaatgaag tccgtgaatt ctcgataata agtgacattc
     7801 gaaaaaacaa gcaatgtcaa aagaaaataa atgaccatca tcgtgatata agcaactcta
     7861 gggcgtctga tataaagagc aattccaagt aataacaatg tagttgcaat agggttgata
     7921 aacaagataa agtattgaat gccactttcg attccgagat ggaaatcaac taaataggca
     7981 aaaatcgttt tcaaccaaag taaaacgact agtaaagaaa aaaatccgag cctcttattc
     8041 aaaaaggcag gtgtttttat acatttcaag ataatcttcc tttctgcact tcttaatcac
     8101 agaagtgtcc tgaaaccatg ttacacagct cacttattct catttatttt actagtatga
     8161 caatatagtg tcaattgaaa ccagaagttt atgctgtttc attaaggttt tctttaagct
     8221 tcatgaaaaa atgagcagta agacaagtcc tatgtttttt gttatacttg ggaagagaaa
     8281 gctaaagcag gtgaaggaat gaaagtaaaa gtaaaaaaaa gagcaagcaa tcgattgaaa
     8341 aaaggatatc cactgatcca aaaagaagac cttgctcatc cccaaaaaga aaccacgcaa
     8401 tgggtggaat tagttgattc gcaaaaccaa tttatcgcaa aaggatatct aggaaagcag
     8461 aataagggaa tcggttgggt attgagccag cagaacatcc catttgatca atcttttttt
     8521 gaagaatttt ttgtgaagtc aaaagaaaaa agaactttct ttttttcaga tgattctact
     8581 accgcttttc gtatattcaa tggagaagga gacgggatcg gcggtctgat tgttgattat
     8641 tatgcaggtt atgctgtttt ttcttggtat aacgaaacgt tatatgcaca tcgtgaatta
     8701 ttgattcaag cttttcgtga aatctacccg gaaatcatag gaggctatga gaaaattcga
     8761 tttgaatcag taaatctgcc tgagagccag ttcatttacg gaaaagaggc accggaaccc
     8821 ttgatcgtaa aagaaaacgg agtaaaattt gccgtttatt taaatgaagg attaatgact
     8881 gggatctttt tagaccaaaa agaagtacgg ggatcattag cagaaggttt cgcttctggg
     8941 aaaacagttt tgaacatgtt tagttatacc ggcgcctttt ctgttgctgc cgcaatggga
     9001 ggggcaatcg aaacaacaag tgtcgatcta gcaaaacgca gtttaaaaaa aacacaggaa
     9061 cagtttgaag tgaatggttt ggatgtcgat cagcaaaaaa tcattgttat ggacgtattc
     9121 gagtatttca aatacgccaa aagaaaacaa tttttatatg acatgattgt attggatcca
     9181 ccaagttttg ccagaaataa gaaaaaagtc tttcgcgtag caaaagatta tggggaactg
     9241 gtcaaggata gtctggatat tttagcagaa gacgggatac ttattgcgtc tacaaatgct
     9301 gcgaatgtct cgatagaaca attccaaaca atgattgaag aggaattcca tgccaaaaat
     9361 gtttcttttg aacaacttgc tttatatcgt ctgccaaaag atttccagac gacggatacc
     9421 tttttagaag gcaattatct gaaagtgttt gtttatcaaa ttaaaaataa ccattagaag
     9481 gagcgatgca gaatggttcg ggtaaaggga ttgaatatag gaagcggtag accaaagatt
     9541 tgtgcgcctc ttatcggggc cgtccgagag gaagtattac gtgaagcagt tcttgcacgt
     9601 gaagcagggg ccgatttagt cgaatggcga gtcgatcatt atcgtgaagt tttttctttt
     9661 gatgaagtag cagaaacatt ggttcttatc catgagatgc ttgatggact gccgttatta
     9721 tttacgtttc gtaccttagc tgaaggcgga gaacaagaca tatcgatccg tcagtatcgc
     9781 gacctttatg aacatgtgat acacacagga ttagtagata tggtcgatat cgagttgttc
     9841 aaagtagaaa gtctaggaaa aagactgata gaggaaatca agtctttaaa cattccttta
     9901 attatcagta gtcatgattt taaagaaaca ccagcagacc ctgtcttgct ttaccgattg
     9961 aatgttatgg aacattttgg agccgatatc ggaaaactgg cagtaactcc caataatgaa
    10021 cgagatgttt tgcggttgat ggagttgact agacgggcaa atgcttttgt ctccatgcct
    10081 attattacga tgtccatggg aaatcttggt aaaatttctc gtctggttgg cggaacgacg
    10141 ggttccgtta tgacatttgg ttccttaagc agcgattcta cttctgctcc aggacaatta
    10201 ccggtcaaag cattgaagca aattattcaa ttattaaatg aaccgttgta gaacaagttt
    10261 gaggtgatat tttttggcaa agaaaaaaat agtaaaaacg aatgcaatcc gtatggtcga
    10321 acaaaaaaag attccctata cagagcacga atatgagtgg gacgaaagcc acctaagtgc
    10381 gtcatcggtc gctgaacagc ttccagaaag ccaatctcgt atatttaaaa cactggttgc
    10441 tgtgggcaat gtgacggggc ctcttgttgc tgtgataccg ggagaagccg aattgaatct
    10501 aaaaaaatta gcaaaagttt cagggaataa aaaagtagaa atgctccatt taaaagattt
    10561 ggaagcaacg accggataca tacgtggtgg ctgctcgcct ataggtatga aaaaactatt
    10621 tcctacttat ttagatcaaa ttgcagaaag ctatgaacaa atcattgtct cggctggcag
    10681 aagagggcta cagatggagt tagcacctca agatatctgt gcattaacga gcggacaatt
    10741 tgcagatata aaacaataaa tcatacgtat aaaagaaaga aggaggccgg gatgttgttt
    10801 gtccagtctc cttctttctt ttttatacat tatgctcttt gcccggcatt cattcctgct
    10861 acatgccctg tagcgaaagc cgctgtgata ttaaagccgc cagtataacc attgacatcc
    10921 agtaattctc ccgcaaaata cagaccatca atgattttac tctccaatgt tttaggataa
    10981 atttccttta gagaaacccc tccgccagtt acaaatgatt tctcaatagg aaaagttttg
    11041 ttgattgata gcttaaactc tttgcatagt tgaacaaaat cttgaatttg tttttgagag
    11101 gcttgttgtc ctgtcagatg atccatttca agccgctcaa gaaagaaaac taacaatcgc
    11161 tcaggcaata taccatgcca agcattgacc aagttctttt tggttgcttt acttttttct
    11221 gtcaatatat gaataagctc ttgtttggtt tgtgtaggga aacaatccag agaaagtgtc
    11281 actggttctc ccgttttctc taattcttta ttgacaaaac ttgaacaacg cagtgcagcc
    11341 ggaccagaaa taccgaaatg ggtgaaaagt aaatccatcg tatgttcagt caatacacga
    11401 ccttttttat ttaaaacgcg taaagtgatg tcttgtaaag aaagcccttg aagtgttttc
    11461 tcctgaatat acggttcatc cgatatcaag ggggactctg tcgcataaag aggcgtcacc
    11521 gtgtgaccca ctcgcttcac gatcttgtac ccatctccag tagaaccagt agatggataa
    11581 gtccgtccac cagttgttaa gataatggca ggagcatgga actcttcgaa ttccgtgcgt
    11641 ataccgtaaa ttttatgttc tttgtggatc aatttctcta ctcgagtaga gaatagaagc
    11701 gtcacatcta gttctttcag acgattgagc aatgcttcga taatcgtttt tgatttattt
    11761 gttacaggaa acatccgtcc atgatcctct tcttttaaat gtacgccttg tgattcaaag
    11821 aagttcatga tatcgaaatt attccattgc gcaaacgtac tatataaaaa ctttccattt
    11881 ccaggaatat gtgcaatcaa atcatcaaca ggacgattgt tcgtaacatt gcaccgacca
    11941 ccacctgtca tcagtagttt ttttcctgct tttttgtttt tttcgatcaa gagtacgcgt
    12001 gcgccttgtt cagctgcact gatcgctgcc atcatcccgc ttgttccagc gccgactacg
    12061 atcacatcat acagtttttc cattccctca cctcaaggaa agtgtagcat atctgaagaa
    12121 agaaaacaga aaaaaatgtt ttgagccaaa agagaaaatg agctaaaatg agggaagata
    12181 agcaaaggag cgtggaacga tgacaaacgc aacagaatat gttcaagcaa ttcaagaaaa
    12241 gctacatcaa aaggatcaag ggcaagaaga atttttacaa gcaatcgatg aattcatgcc
    12301 cacggttatg tcttttttgg acactcatcc cgaatatatc gaaaagaata tcttagaact
    12361 tttaacggaa ccagaaagag ttattcaatt tcgagtgcct tggcaagatg atgaaggaaa
    12421 ctggcgagta aatagagggt accgtatcca atacaactca gctatcggcc cttataaagg
    12481 tggattgcgc ttccatccta gtgtcaatct aagtatcttg aaatttttgg cttttgaaca
    12541 gattttcaag aatagtttaa ccggtctgcc aatcggtgga ggaaaaggcg gaagtgactt
    12601 cgatcctaaa ggaaaatctg ataatgagat catgcgtttt tgtcaaagtt tcatgctgga
    12661 attgtcaaaa catatcggcc catcgattga tgttcctgcg ggagatatcg gtgtaggagc
    12721 tagagaaatc ggctatttgt tcggcgaata taaacgattg aaaaaatatg atactggtgt
    12781 gttgacaggt aaacctttgg atttttgggg aagtaagata cgtacggaag ctactggtta
    12841 tggattagtg tattatgtca aacatctatt gaacgaagaa aaagattcat ttaatcaaaa
    12901 aacagttttt gtttccggaa gcgggaatgt agccatttat gcaatcgaaa aagtgcaaga
    12961 gctaggcggg aaagttgtta catgttcaga ttctagcggg tacatctatg atccagaagg
    13021 aatagatgtg aaactattga aagaagtcaa agaaatcaat agacaacgtt tgacggaata
    13081 cgcaaaccaa cgtccttctg ctatttatca tgctggagaa tctgtttgga cattgaaaga
    13141 aaaagcggat atcgctttgc catgtgctac tcaaaatgaa atagacgaaa gcttggccga
    13201 aatcttagta gaaaatggga tcaaaatcgt agcagaaggc gctaatatgc catgtacagt
    13261 aaaagctgtt tctgttcttc atgaagcagg cgtgtggtat tgtccaggaa aagctgctaa
    13321 tgctggagga gtagcagtat ctgctttaga aatgagtcag aacgtccagc gattgacttg
    13381 ggaaagagag caagtggatg agcagctgga tcaaatcatg gaaacgattt accatacgtg
    13441 tcgccaaaca gcagccgagt acggcgacga aaaaaatctg cttcttggag caaatgttgc
    13501 tggatttgaa aaagttgcca aagcaatggc agcagaaggt ttagtttgaa aaatgaaact
    13561 ttcttggaaa tattgtcagc attttgttta tttgcttgaa aaataataaa aaaaaacgta
    13621 aagtattacc tgtagagtaa acctataagg aggagaacaa atggcacaca ttcattttga
    13681 ctattcaaaa gtagcaccgt ttgtcaacga acaagagtta gggtacatgc aaagccaagt
    13741 aacagcagcc cacaacgaat tacgggaagg tactggcgca ggaagcgact tccgcggctg
    13801 gatcgattta ccaacgaact atgacaaaga agaatttgca cgtatcaaag aagcagctaa
    13861 aaaaatccaa tctgattctg aagtattagt tgttatcggt atcggtggtt cttatttagg
    13921 cgctcgtgca gcgatcgact tcttgaatca cacattcttc aatttattag atagcaacac
    13981 aagaaaagca ccacaaatct tctttgccgg aaattcaatc agctcaacat atattgctga
    14041 cttgatcgaa gtaatcggtg atcgcgactt ttcagtaaac gttatttcaa aatctggaac
    14101 aactactgaa ccagcgattg cgttccgtgt attcaaagag cttcttgtta agaaatatgg
    14161 acaagaagaa gcaaacaaac gtatctacgc tacaacagat aaagcaaaag gtgcagttaa
    14221 agtagaagca gatgcagaag gttgggaaac tttcgtgatt cctgatgaca tcggcggacg
    14281 tttctctgta ttaactgctg ttggtttgtt accaatcgct gttagtggtg cagatatcga
    14341 tgcattgatg caaggtgccg ctgatgcaag caaagcatac tcaagcgaca aactagaaga
    14401 aaacgaagct taccaatacg cagcaatgcg caacattctt taccgtaaag gcaaagtaac
    14461 tgagttgttg atcaactatg aaccaggaat gcaatacttc tcagaatggt ggaaacaatt
    14521 gtatggtgaa tctgaaggaa aagaccaaaa aggcatctat ccttcaagcg caaacttctc
    14581 aactgatttg cactctttag gacaattcat ccaagaagga cgtcgcaata tttttgaaac
    14641 agttgtgaag gttgaaaaac ctcgtaaatc aatcacaatt cctgaacaag cagaagattt
    14701 agacggtcta ggctacttgc aaggaaaaga agtggacttc gtaaatacaa aagcatttga
    14761 aggaacattg cttgctcata cagacggtga cgtgcctaac ttgctagtca aaattccaga
    14821 aatggatgct tatacattgg gttatacaat gtacttcttt gaaattgctg taggtatttc
    14881 aggttacttg aatggaatca acccattcga ccaaccaggt gtagaagctt acaagaaaaa
    14941 catgttcgca cttctaggca aaccaggctt tgaagatctt gctaaagaat tgaacgaacg
    15001 cctgtaagat aagggttttt cgctagattt aaataatcag tgatagaaac atgttcgctt
    15061 taaaatgaaa actggtaccc atttaggtac tcaaacatac tgcatcgttt attcagaata
    15121 aggggctgtg acaagacgtt tgtcacagtc ccttgtttgt atagaaatcg attgattgcc
    15181 ttgctcttta gtttgtttta ggactttaag aacagtgagg ctgacttcga aggttaattc
    15241 tgtttttttg ttttctagaa tcagtaagca aaaaagcact cacttcgagt gcttttttgc
    15301 gatatattct tagcttaaga taggcctaat ttttctttta cagatgaggg taccttatcg
    15361 ctggtaatta cttttttctc tatgatccgc ttttcgcttg ctttcaactg caagatcgtt
    15421 ccttcggggt aagcagaacc attggcagag tcaccagaag taatgacatc gaaatctaac
    15481 tgtctttttt caccgttttc agcataagca tctacgttgt atttaaaacc tcttccaatt
    15541 acttccccat tatctgcttt gatatcagtt tctgcaggca ttggagcttt gactaccgca
    15601 taataatctt tacctacata agtagactga tagtagtccc aacctttcca tcctataaaa
    15661 gcaatcaata tgataacgat caaccctaaa atttttttca tgaaagatct ccttttttat
    15721 ttttatta
//
LOCUS       DACEUZ010000060        15710 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.060, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000060 DACEUZ010000000
VERSION     DACEUZ010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15710
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.060"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            52..927
                     /locus_tag="IXG44_001573"
     CDS             52..927
                     /locus_tag="IXG44_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1658296.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            1031..1795
                     /locus_tag="IXG44_001574"
     CDS             1031..1795
                     /locus_tag="IXG44_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HAQ1658297.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            1812..3224
                     /locus_tag="IXG44_001575"
     CDS             1812..3224
                     /locus_tag="IXG44_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1658298.1"
                     /translation="MNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADDMLNIPVIGAA
                     GNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMDPEVNMGIFGG
                     IISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLGFIWPLIQIGI
                     DNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSPSKEVFRGEMT
                     RFINGDPQAGLFLSGFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMGSGAGTSFIAN
                     ITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVDYLINFRIATN
                     AILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEETELGLASKNYE
                     YMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISGIIKPTEKNYQ
                     IIIGLEVTYVMAEFNKLLEE"
     gene            3241..4566
                     /locus_tag="IXG44_001576"
     CDS             3241..4566
                     /locus_tag="IXG44_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005227470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HAQ1658299.1"
                     /translation="MKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDEER
                     NMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKIPL
                     KHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVRRK
                     YPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREIMP
                     EIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVVEH
                     TDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLNDDR
                     RRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAVEK
                     LLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            4579..5037
                     /locus_tag="IXG44_001577"
     CDS             4579..5037
                     /locus_tag="IXG44_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAQ1658300.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            5329..6630
                     /locus_tag="IXG44_001578"
     CDS             5329..6630
                     /locus_tag="IXG44_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1658301.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            6657..6917
                     /locus_tag="IXG44_001579"
     CDS             6657..6917
                     /locus_tag="IXG44_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAQ1658302.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(7916..9151)
                     /locus_tag="IXG44_001580"
     CDS             complement(7916..9151)
                     /locus_tag="IXG44_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAQ1658303.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            9719..10546
                     /locus_tag="IXG44_001581"
     CDS             9719..10546
                     /locus_tag="IXG44_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1658304.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            complement(10819..11589)
                     /locus_tag="IXG44_001582"
     CDS             complement(10819..11589)
                     /locus_tag="IXG44_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ1658305.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            11807..12874
                     /gene="ulaG"
                     /locus_tag="IXG44_001583"
     CDS             11807..12874
                     /gene="ulaG"
                     /locus_tag="IXG44_001583"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HAQ1658306.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            12892..13353
                     /locus_tag="IXG44_001584"
     CDS             12892..13353
                     /locus_tag="IXG44_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ1658307.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            13373..14857
                     /locus_tag="IXG44_001585"
     CDS             13373..14857
                     /locus_tag="IXG44_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAQ1658308.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQVLIFMGLILGCYWAVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            14900..15199
                     /locus_tag="IXG44_001586"
     CDS             14900..15199
                     /locus_tag="IXG44_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1658309.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            15214..>15710
                     /locus_tag="IXG44_001587"
     CDS             15214..>15710
                     /locus_tag="IXG44_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate-6-phosphate decarboxylase"
                     /protein_id="HAQ1658310.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTG"
BASE COUNT         5023 a         2399 c         3279 g         5009 t
ORIGIN      
        1 tttcttaggt cgttttttgt ctttaaaaat tgtttggaga caaaaatatg tatgaaggag
       61 tctgcaagtt gttttgtttt atcatataga ggaggtgaat taacggtgta tgtgatgaaa
      121 aagctgaatc ataatgctgt attgatcgat gacgatggaa tagaaaaaat tgcgatgggg
      181 aaagggattg gcttttctgc taaagtgagt aagccatttg atgttgagga tgctgacaaa
      241 ttatttgtat tagattctaa agaaaagatc aaacgattca gtgagatagt ctcgcagatt
      301 ccaatcgaat ttatggattt tgcggaagaa gtgattgaac tgataacagc taggatctcc
      361 tctccattag attcaaatat ctatattaca ttgacagatc atatttattt tgctattcaa
      421 cgaaaaagag aagatagaga tgtcacagca attatgttgc cagaaatgag attcctttat
      481 ccaaatgaat atgatatagc agtagaagtg gtcgagcgaa tcaatcatcg atatcaaacc
      541 gagttagggt caaatgaagt aggattcatc actatgcata tcattaatgc tgagctagga
      601 gagcctaata gtttaaagag cttaaaaata gtagaaatga cgaccacgat tttgtcccat
      661 ctagaaaaat cctattcaaa cgtttatgat aaagaatcac tgacttatca tcgcttaatg
      721 gttcatatca aattcctggt taaacgcttg atttatcagg aagaaacgcc agtagaaaat
      781 attggttttt ttactgaaac atttaaacat agcaaagcgt attgtacagc actaaagatt
      841 aaagatatca tcgaacagca atttcaactt gccattcaag aaaatgaaat catctatttg
      901 gcaattcatc tagcaagaat ctattaaaat aagggcgtaa ctatctatag gcgcaaccaa
      961 atgacacata gtaaatggac tatgattatt tggttgcgcc tattttagta gaaaaaagaa
     1021 ggagaatgaa atgggaaaag tgatttttaa ttcggatgat tttggctata gccatggtgt
     1081 gaattatggg attatggatg cttatcagcg aggaattttg acatcaacta cattgatggc
     1141 aaatatgccg ggtttcgagc atgctgtgaa actaagaaag gaaatgccta ggctaggtgt
     1201 aggagtccat ttaacattga cgtgtggcaa accactcctc aaaactgtcg atactcttat
     1261 cgaaagcgga gagttcagat ccttatctta ttatcagcat ccatttaaca ttgacaaaga
     1321 tcaactgtat caggaatgga atgctcagat tcaaaaaatt tatcatgcgg ggatcatacc
     1381 aactcattta gacagtcatc atcatactca tactttggat gaaaatcagg aggtagttgt
     1441 tacattagca aaaaaatatg atttgccggt tcggagcaac tttgaacgaa aagatgaagt
     1501 tcgtcatgtc gattattttg agccttgttt cgatgtcgta ggagaaactg atgaaaaaaa
     1561 acgacaaatc aatacgagtg tggaagaata tttggcaagt ttactcatga tattgagaga
     1621 agataaaacg atcgaagtca tgtgtcacgc agcgtatttg gatcaggaat tgttaaatgg
     1681 ttcaagtttt gtgtatcctc gtacccacca agtcgaattt ttaattcatt cagcatttgc
     1741 tcaaaaagtg aaaaatgatc ctttgattga gttagtaaca tatgaggata tttaatagag
     1801 gaggaataaa aatgaatgtt aaatcaaatg tgcagaagct tggaagagcg atgctacttc
     1861 cagttgcagc gatgccatta gcgggattga tcatgcgttt atcagctgac gatatgctga
     1921 acatcccagt aattggtgca gctggaaatg ccgtttttgg aaacttggat atcttatttg
     1981 ctataggagt tgcaatcggt tttgtcaaaa ataaggacaa aggaatacct gcacttactg
     2041 gttttttagc gattgctacc ttgaaaaaag gcttagaaat tatggaccca gaagtgaata
     2101 tggggatttt tggtggaatt atttccggtt tagtagcggc atggacgtat aatcgcttta
     2161 aagaacaaaa attaccagta gttttttctt attttgcggg cgaaaaattc ccattgacta
     2221 tggtgatgat tctacaaaca atcgcagctg tttttttggg ctttatctgg ccgctcatcc
     2281 aaattgggat tgataatttt gcaaaactgc tcgttgatat gggagcatta ggtgtgggga
     2341 tatttatgtt tttgaatcgt ttgctgatcc cctttggact gcaccatgtg ttgaatacgt
     2401 atatttatta cgatttagga agctatacat ctcctagcaa ggaagttttc agaggagaga
     2461 tgacacgctt tatcaacgga gatcctcagg ctggactgtt tttatcaggt ttttttgtag
     2521 taatgatgtt tggtatacca gctatatgtc tagctatcta taaagcagct tataaagaaa
     2581 ataaagagat ggtcaaagga atcatgggaa gcggtgcagg aacttcattt attgcaaata
     2641 tcactgagcc tgttgaattt agtttcatgt ttctttctcc tatgttgtac gtgatccatg
     2701 cactttttgc cggtttagca gggattgtct gttacttatt caatatacga attggtttta
     2761 cattcggagc atgtattgtt gattatctca tcaattttcg aatcgctacc aatgctattt
     2821 tgattcttcc aatcggaatt ttcttttttg ccttgtatta cgttactttt tattatttga
     2881 tcaataagag aaacattcag acattaggac gtgaagctaa ggcagagttt ggcaatgaag
     2941 taaccttaga agaaacagag ttagggttag caagtaaaaa ttatgaatat atggcaacaa
     3001 aaatgctaca agcgtttgga ggaaaagcca atatcctgga tgtctatagt tgtaatacac
     3061 gcctacgggt agaggtagtg gatcccacca tggtcgagga acagcgaatc aagcaattag
     3121 ggataagcgg gatcattaaa ccaactgaaa aaaattatca aattatcatc ggcttagaag
     3181 tgacttatgt tatggcagaa ttcaataaat tattagaaga atagaaagga aaagatgatg
     3241 atgaaaaaac agattatcac tatagctgga ggcgggagta cgtatacacc aggaatcgtt
     3301 caagccattt tgaacaatga aaaacggttg ccattatctg aaatacgttt atatgatatt
     3361 gatgaagaaa gaaatatgga tatgtacttg attgttaaat ttatgctgaa aagaaaaggt
     3421 ttttcaaata ttcgcattcg tgcaacggat gatccaaagc ttgcttttac aggatgcgat
     3481 tttgttttct cacaaattcg tgttggagga ttggagatgc gagaaaagga cgaaaagatt
     3541 ccacttaaac atggattagt aggacaagaa acgtgtggac tcggaggatt tgcttatggg
     3601 atgcgctcaa tcaaaggttt attggaaatt gtcgatcata tacaagacta tgcccccaaa
     3661 gcatggattt tgaattatac aaatccagag tcgatcgttt ctgaagctgt tcgtcgtaaa
     3721 tatccgaatg taaaaatgat taatgcctgt gatatgacta tttcaattga agaaacgatt
     3781 gcagtgaatt acggttatga tcgtaagaat tggatcgtta cgtattatgg tttaaaccac
     3841 tttggctggt atacatcgat ttatgataaa gaattgcaac gagaaattat gccagagatt
     3901 attgaaaaac taataacaaa agaaatgcaa gtcgccgatt ttaatatagg agataaaact
     3961 tggcaaaaaa cctttcaaat gatgtcagtc atcacgaaaa attttccaag taatatcccg
     4021 aataattact tagagtatta cttatatcct gatatggtcg ttgagcatac tgacaaagag
     4081 tatacacgtg ctaatatggt catggatgga cgagagaaaa ataccaaaga aatggctgat
     4141 aaaatacgcc gcggtattgt ggaagaagta ttgaacttta attttgggga gcatggacaa
     4201 tatatcgtcg atatagctat ttccttgttg aatgatgatc gtcgtcgctt tatgctaatc
     4261 gttcctaatc aaggggcgat tccgaatcta cggtcagatg cagttgttga aataccggca
     4321 tatgttggtg caactggtgt ggagccaatt acactaagaa aaccaatcag tgattttcat
     4381 aaaggattga tggaggcaca agttgcagta gagaagttac tagtagatgc ttactttgaa
     4441 ggttcttatc aaaaagcttt acaagcattt acattgaatc aaaccgtacc aaatgccaga
     4501 gtcgcaaaaa aaatactaga tgaaatgatc aaagtaaaca aaaaatattg gccagcatta
     4561 aagtagaggt gataaaaagt gcatttcttt aaaacaagaa acattctaaa agctgtagct
     4621 agtggtcaaa ttattccatt aacagcagta aaagacgagg tcttttcttc aaaaatgata
     4681 ggagttggat acgctataac gaatcatgat ggatatatcc atgcgccagt taaaggaata
     4741 atcgataata tatttcctac ccgacatgcc ataacgatcg aaagtgaatc tggcagaaaa
     4801 atactgatcc atatgggttt agatacagtt gaactcgaag gaagtccgtt tcagatacgg
     4861 gttgaaagta accaggaggt aaaagaaggg acactgttag ctattataga tttgaagaag
     4921 ttggagcaag cacaaaaaga tacggtgatc attgtagttt ctccaggcat ttttgaagga
     4981 aatttattaa aacaaaacca agaagtaaag attaaagata gttgttttga attttagtgt
     5041 acgatgaaaa aagttaaata gggtgatatt tatgggtgaa tttacaaaat ttttttgaaa
     5101 tgatatgttt caatcactag atttaatcat tttgattttt gttaattatc gaattttttt
     5161 gttgctccta tgatttagaa aacttatttt caggattctt aaaaataaat ttgattattt
     5221 aagagaatat attgaaataa aatttcttaa ttatataatt agttatgaac attctttgcg
     5281 ttgtcatgca atgaatggtt catattttat tgaaaaggag tggttttagt ggaaaagctg
     5341 atgaaatggg tcgaagagaa acttgtccca ccaatggcaa aaattggtac acagcgtcat
     5401 ttattagcaa ttcgtaatgg ggttgtttca acattgtcat tgattttgat tgggacattt
     5461 tttatggtct ttatcaattt accgtttcct ggatggaatg aatttattgc cccttattcg
     5521 gcaacgattg ttttaccctt taggatgacg atgggattga tggctattta tgcaactttt
     5581 gttatgggtt cggatctagc taaatcatat ggattagata gtgtgacagg tggtatatta
     5641 tctttaggaa cgttctttat gttacaagtt cctgtaaacg ttttaactcc tgaagaggca
     5701 cctcttggct gggtcttacc tatgagttct ttgggagcat ctgggatgtt tgcggggatc
     5761 ttatctatga tttttgcagt ggaagtatat cgtttcttta aaaatcgaaa tattactatc
     5821 aaaatgccag aacaagtgcc acctgctgtt gcccgtagtt ttgaggcatt gattccaggt
     5881 gcagtcattt taacaattac ttggctgatt cgaagcgtcg cgggctttga tgtaaacgct
     5941 gccttattat cgttattcga gcctttaacc aatattttag ggaataattt gcttggtgtt
     6001 ttactaccaa tgtttttgat ccatttgttg tggtcatttg gtatccatgg catgagtatt
     6061 gtaggggctg ttgttcgtcc aatgtggctg atcatgttgg atgagaatgc aaaggcattg
     6121 gcagatggta cagctgccac ccaattaccc tatatcgctc ctgaacagtt ttatcaatgg
     6181 acggtaacaa ttggtggtgc aggagcaacg atcgtggtat ctgttttgtt tatattcttc
     6241 tgtaaatcaa aattcttgaa agaggttggt cgtttttcta ttattcctgg tatcttcaat
     6301 atcaatgagc ccataatttt tggagctcct atgatgttga atccttacat gttcatcccg
     6361 ttcaacttag ttcctttagt tttgactatt atcagttatt ttgcagtcaa attagaaatg
     6421 gtcaatagat ttactgtttt gccaacatgg acattaccag ctccaatcgg cggttatctt
     6481 tcagcaggaa atgactggcg tgttgttatc ttgatcatga tcaacacatt gatagcattt
     6541 attatctatt atccatttgt aaaagcttat gataaaaaga tgctgtcaga tgaacaaagt
     6601 cgtggagaaa acgaagtcca gactgtctaa gtttggtttt tagaaggtgg gaaacaatgt
     6661 attggctgat tttatttttt gtttttatct ttttattaac ggctagtcac cttatattga
     6721 atatgctagc tacatatcat attcaaatca atcgatggat ttgggcactg gcatcatttt
     6781 tgatcgtgat tcttccaaag atcattgtac cgcatatgaa tgttttgttt tcatggggga
     6841 catatgtact ttgtggaatt tttgctatca attttatgat cgagcagcat agatggtttg
     6901 tcacatcgaa gctttaaatg ttctaaaagt ggttgtaaca gaagtggtta gcttcaagaa
     6961 ataagaagaa atttacgaaa attgcttctt aaatttttgt gaaatgttgg cttatttttc
     7021 gtttggttga acttattacc gcagatgcaa gcttttgacc accgtttatt cggaatatgg
     7081 gactgtgaca aggcttttgt cacagccctt ttttacataa agattattat tgttttaact
     7141 gttttggcag aaagaaggag gaaaaaatgg aaattttatc agcaatggat ttagaattag
     7201 tagatctact accaaagtga tcaagtcaaa tatagatttg ggagcttaca accatatagt
     7261 aggggctgca gtatggtgta tgaatggtaa tatgtatgat gtgagtgttt attcaaatca
     7321 tgaaacgtgt atagaaataa ttgtgctggg aatagcaatc agtcatggtg aacgtgactt
     7381 ttagtgtatc gttgctgacg acatagaaca ttctgacatg atctactcaa tatgtgggga
     7441 ttgctggcag tttatatagg agtatgcatc ggattgcgag atcattgtct caacagaaga
     7501 gggaggttga ttatcaaaga taaagtggta aatcctacat ttgatttagt aattgttgag
     7561 gggactagcg gcacaaatct ctacaaagcg atgcttctag atctatatga cacattttaa
     7621 gaaaatgtat ttgcctaatt attatggtct ttcttttgaa gaaacgttga gatgatccaa
     7681 ccagagaaag gtgtcaaaga atttgactaa gaataaatgg cagacgctac ctatgctcat
     7741 ttggatagcg tcagatcttt tattttaagg atgattttgt gttttttaga caccgtaatt
     7801 taagtaactc taacttttca atccactatt tacttttttt gtattttcac tgtctgggtc
     7861 tcgaatagcg aaattactga tgagtctcta ttaaaaaagc tttttctact tgaaactata
     7921 ttgggtggtt gctcaggaag gtttcgattt cttgaattgc ttgttgtgga ccatttattg
     7981 gaaaaacggc ttgcagcgtg cgtgcctgct gacgaatctt gggtgaagaa acttcgcgca
     8041 gagcaatctg cagttttctt tcagtcactg gttttgtgag ggcgattgcg tttccgtatt
     8101 ttacacaata gtcaatatta cagctttgct caaattgcat ccccacaccg ataaacggtt
     8161 tgccggattc acaggcagtt tggacggtcc cttccccgcc gtggataaaa gaaaaatcaa
     8221 tgaattcatg caaaagatga ctagacagat attctgttat taaaagatct tctgagtcag
     8281 attgaatgtc atcaagcaga tagtcaatag gtgcaataac cgctagatcc ggttgttttt
     8341 gtaagaagaa caaaattttg cgtaacatac gcggattgcc tgaactaccc attgcgaaat
     8401 agataatacg ttttctttct tgttgctttc gctgtataaa agttagcact tcttcaggca
     8461 aggaagtact gagcttagca aaaattggac caacgattcg ataattttcc ggcagctgtt
     8521 cttcttccaa aaatagttta ggcgttgtga tgagatttag atcagcatcc atcatttgta
     8581 aacttgtttt tggtaaagga agcatttctt tttgtgcagc gtgtttgaag gcattgggga
     8641 tccatcgggt atttactata cgcttttttg tccagtgcca aaggggattg cttaaagggc
     8701 cgaagaaacg taactcgtgg ggcatcagcg catgctctga ttgaaaaaaa ggttcactca
     8761 aagcataggg tttaacatat actaaaggaa tctttttatt gcgggccgat aaaaaaattg
     8821 tcacattcga acctgtcaca atcagtcgag gctcaaactt agcaatcaga ctctgttcac
     8881 tagtgatacg agaggcaacc atttctttgg taaaagggtt tttaagtgta cgcaactggt
     8941 caaattttat gatttgttct tcttgttttt tcgtaaggat cggcgctagt aaatgatact
     9001 caaagccact ttctgtgatc aaaggagcaa aagttttaga aaagccaaag aatacacagt
     9061 aataacggtc tttcatagct ttggcaacct caatcattcg tgttgtctca gctaaattga
     9121 atactgcagg agcaaaaaga attgtcttca tgaaatcctc cttattggaa tgagtctatc
     9181 ccttcgtttc ttactagtat actaaaaaac acagcgttcg agtagcctat gtatattttg
     9241 aatattaaac tgtctctcgc tgttttcaca acttctaaat ttaaattttg taatcaagtt
     9301 gaaactccaa ttttcaaaaa aacacgcaac aattttttga tcgatttata tgttgtgcca
     9361 gataaatacg agaagttgtg tcattaaaaa taaacatgag aacataaaaa aataaaataa
     9421 ttatattaat tgataggtgt gctgtcgtca ttatacacac gtaagaaaga aatagttatg
     9481 tttaaaaata ttcttagact ttctcgccat gacaattttc actatgtgtt ataactgtaa
     9541 acgataatta ttcagataac acaaaaagaa atcaaggagc agatacgcca gttgtttaat
     9601 ttgaatatta ttttaaaatt aaaattttta ttctgttcta aacatttttg gttgtttaga
     9661 actttttttt gtgtatgata taaaggagaa ataatttttt aaagggaagg aaattgtttt
     9721 gattaaagta gatggagcat tactaaaaga ataccgaaag aaaaaggggt taacacaagt
     9781 acaattagca gaaggcattt gtgaacaagc aactgtcagc aatatcgaaa acaagaatat
     9841 gtcgccaggc atcaatatct taagtgcgct ttgcaaaaag ctgaatgtcg agatctccga
     9901 tatattagta gaaacagaag aagacaaaat caggaaaaaa ataaatgaga ttcaatcatt
     9961 gaattctgta tcaaaatacc aagaatctta taaaatttta caaacgatcg atcaggaaaa
    10021 agtaagtaat tctgatcttt taaatcaaat tttattttat caaggactgc ttgatcatct
    10081 ttatgcgaaa aaaccagatg cagcattatt ttattttaat caagtgttgg aacaaacgac
    10141 cgaaacagat atccatcact tacaagcagc tgcaaacgtg gcaatgatct acttgtcaaa
    10201 aggagaatta gattttgcga aagtttatgt ggagcgtaca cttaaaatat tgagtgagac
    10261 tgattttgat aatctgatgg tctgcattgt atattatgat atcgccacct attaccgtaa
    10321 aatcgaggat tatgataaag caatccaatt atgtgagaag ggtattgagt ataataagaa
    10381 acataaatcg acttatgcat tggaatactt gctttatgaa attgcttctt gccataagca
    10441 attaggagaa gatgattatt tagaaagata tatggacgca aaaaaaatag cgcgtttcaa
    10501 cggaaatgat tacgcggtaa aggtgattga aaacgacctg aaataaagtg gttgtgaaat
    10561 cagtgttctg atttgaatac cgtttattcg aaaaaaggag gtgtgacttt tgtcatacct
    10621 ccttttttcg atattgtttg attcctattt aggcaacata taacggttat aaaagaagca
    10681 atagattatg atggtatcga ttggatggat acatcttggg attcctttat caatttgagc
    10741 gtagcgtgtt tttcatttat cgataaattt gttattcatt tttttgataa tcctatttac
    10801 atatttacgt cctggttctt aaaatgaatc tacttgaatg gtttttatcc ccgtatttct
    10861 gatctcctct aatacttttt cagaacaaaa tttatcagtg atcaaataat caaagactga
    10921 aatatcagca atcttgaact ttgatgtcag tccgattttt cgatgatcag cgactaaaat
    10981 cttacatttg attgtcttgt tgaccatcat ttcgttgatc gttgattcat ttaatatctt
    11041 tgttgtgacc ccatgtacta catcgactcc gctacagcca atgatacaaa tgtctgcatt
    11101 gactgaagaa atcgtattta atgcgatgtc gcccactagt gcttcttttg gaaagcgcat
    11161 ctctccacca gttaaaatat aactacaatt atctttgtgc ggagagtaat tgattttcac
    11221 gttgtttgta atgactgtca aatggttaga ttgaagaaaa tccagtgttt tcaacgcagt
    11281 cgagctagaa ttgataaaca gcgtatcata atcgttaatg tatttactag cttctctggc
    11341 aatcgcttgt ttaatccttt caggaccaga ttgatcaaaa tcagtctggt ttttgtaatt
    11401 ataagaacaa taaccgtaat tgcgtatgat ctcgtttttt tcttctaata aatttaaatc
    11461 tcttcgtatc gtactaatag atacatcaag ggattcagct aattcgaaag ttgatagttg
    11521 ttcagtctgc tgaagcagtt ccaaaatttc tttttgacgt ttttcaatat tgacaagtga
    11581 gtttttcatg acttttgcct cctgagggac atcttgatcc atggatttat ttgactagtt
    11641 gttcatttaa acattcatat ctgtctttat tatactgaaa caatcattga ttactgaaag
    11701 aaaatcatat aaaaaacaaa cggtcatata aaagggtttt catggatgct gatacacatt
    11761 aatatttact tgtaagttgg aacaacgata tggggggagc aagcaaatga gtaaaactgt
    11821 caatgaagta acaaaagaaa agtggttagt agaaacattt cctgagtggg gcacatactt
    11881 gaatgaagaa atcgaaacag aagtagtgga tcctggaact tttgctatgt ggtggctggg
    11941 ctgtacggga atttggctga aaagtcatga aggaacgaat attttatgtg atctatggtg
    12001 tgggacggga aaacgtagtc atgggaatgg catgatgaaa aaaggccatc agatgatgcg
    12061 tatgagtggc gttcaaaata tgcagcctaa tttacgtact cagcctttcg taatcgatcc
    12121 ttttgcgata aaaaatgtag acgctttaat cgttacacat atccattccg atcatctaga
    12181 tatccataca gccgcagcag ttcatcaaaa ttgtcctgaa gcaacattca ttgggccaaa
    12241 agaagtggtt cgcacgtggc aaaaatgggg aattcctgaa gaaaaaacga ttacagtaaa
    12301 gccaggtgat gcagtcaaaa tcaaagacat caatgtagta gctttagaag cgtttgatcg
    12361 gacagcgttg gtcacttgtg aagatccaga agaggtatta aaaggaaaaa tgcctcagga
    12421 gatggatcaa attgcagtaa actatttatt cgaaacatca ggagggaatc tttatcatgc
    12481 aggagattca cattactcca atttatttgc caaacatggt aatgaacaca aaatcgatgt
    12541 ctgtttaggg gcctatggtg aaaacccacg agggataaca gacaaagtta cttctgttga
    12601 tatgctgcgc atggcggagt cattgaatgc aaaagtagtg attcctgtcc actacgatat
    12661 ttggtctaat tttatggctg atccaaaaga gatcactgaa atttggaaat tcaaaaagga
    12721 tcgcctgaag tatggattta aaccttatat ctggcaagta ggcgggaaat tcatctatcc
    12781 gacaaacaaa gatgatcttg agtttaattt ttatcgagga tttgaagatg tcttctcgat
    12841 tgaaaatgat acaccgtttc cgtcgttttt ataagaaaga aggggagttt cttgctgaaa
    12901 tacttttttg accatcaatt gattcattac ccagataaag aaatcacgga ttggcgtgaa
    12961 gctatcgccg aaagctgtct tgtattgatc gataaggaaa ttattgatga aacgtatgtg
    13021 gaggagatca tccattgtgt agaaaaatac ggtccttata ttgtgatcgc acctcaagta
    13081 gcaatgccgc attcttcaga aaaaagtgat ggtgtttttg gaacagctat ttcatttacc
    13141 aagttgaaac agccaatccg ttttgaaagt gaaggggaga atgaggagaa aacggctagt
    13201 ttgtttttta cactcgcagc aaaagatcca gaagaacatc tagaaaatat ccaaaaatta
    13261 tccgaattat taatgacaga tggattggtt gagcgtctca tctctacgaa ctccatcgat
    13321 gattaccaag ttgtgatggc agaatataat taattgatag aggagaaaaa agatggggga
    13381 atttttgtta agcatttgga cgtattttgc aacaaatatc ttgacgcagc cagcatacct
    13441 gattggtttt atcgtgttat taggctatat tttgctgaaa cgcccgcttc atgaatgcat
    13501 cgctggattc ttaaaggcaa cggtcggtta ctttattttg actgttggct ctggtggttt
    13561 ggtcaataat tttcggccaa tcttggttgg actaaaggaa cggttcaatt tggatgccat
    13621 ggttacagat ccttattttg gacaaaatgc tgtggatgct ggattaatgg agacatttgg
    13681 ccgtaccttt ggagatgtga tgattttatt actgattgca tttgttttaa atatcttatt
    13741 agtccgtttt cagaaatata cgaaactgcg ttccgttttc actaccggaa acgttcaggt
    13801 tcaacaagca gctaccgctt tttggatttt actgttttgc tttcctgatc taggtaggat
    13861 acaagtattg atttttatgg gactgatttt aggttgttat tgggcagtag cttctaactt
    13921 aacagtagga atcacgcagg aattgacaga aggtgccgga tttgcagtgg cacatcagca
    13981 aatgttcgga atctttatat ttgcaaaatt agctgagtgg atgaaaaagc gggatgaaaa
    14041 gaaaaataga tcaatcaagc aagacaaaaa aattgaagat atcaaattac caggattctt
    14101 gtcgattttc aatgagaata tggttgctac ttcgttactc atgctatttt tctttgggat
    14161 cattctgatt gtactaggca aagattattt gattcaagcg caattcatgc aagaagggca
    14221 gagtttctta ttctacatta tgacgacttc tttaaatttt gcggtatatc tggcgatctt
    14281 gcaattaggt gttcggacat tcgtcgatga actaactcaa tctttccaag gaatctcaaa
    14341 cacgatttta ccaggagctg ttccaggaat tgacgtagct gctacttttg gctttggttc
    14401 gccaaatgcg gtaacgattg ggttcttgtt tggtgctttg ggacagtttt tgatgattgg
    14461 tttactgatt ttattgaaat caccaacgat cgtcatagcc ggtttcattc cgttgttctt
    14521 tgataatgcc gttatagcag tttatgccaa taaccgaggt ggattcaaag ccgcttgcct
    14581 gttcccgttt ctttcaggaa tcatacaagt aggcggttca gcattattcg ctacatggat
    14641 tgggatgtca acatacggtg gttatatcgg gatgttcgac tgggcaacag cttggccatt
    14701 gtttacagta atcatgaaag tcctaggtta tgctggaatt gccgtagttg tcattggttt
    14761 attggctatt ccgcagttac aatatcggaa aaatcctgat ggttattttt tgattgtaga
    14821 agactatgac acatatgcag aaaagcagag agtataaaag aaaatctgat tagacaacac
    14881 tcataatgga gggattaaga tgagaatttt agtatcgtgt gcaaatggat caggaacaag
    14941 cttgatgatg atgagaagtg tggaaaaagc aatgaaagaa ctaaacgtac caattaccaa
    15001 aatccatcat tgtgccattt cagagggaaa aagttcagct ggccaatacg atgtagtttt
    15061 tacgccagtc aattttttga atatgtttac tcaggcagag aaaaaaggag ttacagttat
    15121 cggtatcaaa aatgtgatgt cagtcaaaga aatcagtgaa aaatttgctg aaacaaagtg
    15181 gtaccaaaaa aacaagtaaa ggaaggaact aagatgagca ggccgaattt gcaaattgcg
    15241 ttagatcaca atgacttaga acatgcatta ggagatgtta tgaaagttgg tgatgtagta
    15301 gatattattg aagtgggcac aattctttgc ttacaggaag gacaaaaagc gattcgctgt
    15361 attcgcagta tgttcccaga taaaaaacta gtcgctgata cgaaatgtgc agacgctgga
    15421 ggaacagtgg cgagtaatgt tgcaaaagca ggagcagact ggatgacagt catttgttgt
    15481 gcaacgattc caactatgga ggcagcacaa aaagaaatag gtgaattgca agtagaactc
    15541 tacggcaatt ggacttttga acaagccatg gattggcata acatcggtat ccggcaggta
    15601 atctaccatc aaagccgtga tgcgctgtta tctggtgaaa cttggggtga aaaagattta
    15661 tcgaagatca aaaaactgat tgaactaggc tttaatgttt ctgtaactgg
//
LOCUS       DACEUZ010000061        15457 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.061, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000061 DACEUZ010000000
VERSION     DACEUZ010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15457
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.061"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(403..1635)
                     /locus_tag="IXG44_001588"
     CDS             complement(403..1635)
                     /locus_tag="IXG44_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAQ1658311.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSAAGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVIDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            1892..2461
                     /locus_tag="IXG44_001589"
     CDS             1892..2461
                     /locus_tag="IXG44_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1658312.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(2639..3079)
                     /locus_tag="IXG44_001590"
     CDS             complement(2639..3079)
                     /locus_tag="IXG44_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HAQ1658313.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLSGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            3237..4001
                     /gene="map"
                     /locus_tag="IXG44_001591"
     CDS             3237..4001
                     /gene="map"
                     /locus_tag="IXG44_001591"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HAQ1658314.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            4033..4956
                     /locus_tag="IXG44_001592"
     CDS             4033..4956
                     /locus_tag="IXG44_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HAQ1658315.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            5032..6171
                     /locus_tag="IXG44_001593"
     CDS             5032..6171
                     /locus_tag="IXG44_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HAQ1658316.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFTAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            6164..6964
                     /locus_tag="IXG44_001594"
     CDS             6164..6964
                     /locus_tag="IXG44_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ1658317.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            6964..7791
                     /locus_tag="IXG44_001595"
     CDS             6964..7791
                     /locus_tag="IXG44_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ1658318.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            7769..8503
                     /locus_tag="IXG44_001596"
     CDS             7769..8503
                     /locus_tag="IXG44_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ1658319.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            8603..9469
                     /gene="rfbA"
                     /locus_tag="IXG44_001597"
     CDS             8603..9469
                     /gene="rfbA"
                     /locus_tag="IXG44_001597"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HAQ1658320.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            9483..10055
                     /gene="rfbC"
                     /locus_tag="IXG44_001598"
     CDS             9483..10055
                     /gene="rfbC"
                     /locus_tag="IXG44_001598"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HAQ1658321.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            10077..11105
                     /gene="rfbB"
                     /locus_tag="IXG44_001599"
     CDS             10077..11105
                     /gene="rfbB"
                     /locus_tag="IXG44_001599"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HAQ1658322.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            11203..12054
                     /gene="rfbD"
                     /locus_tag="IXG44_001600"
     CDS             11203..12054
                     /gene="rfbD"
                     /locus_tag="IXG44_001600"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HAQ1658323.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            12088..14121
                     /locus_tag="IXG44_001601"
     CDS             12088..14121
                     /locus_tag="IXG44_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1658324.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPAKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKIDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKEIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            14165..15445
                     /locus_tag="IXG44_001602"
     CDS             14165..15445
                     /locus_tag="IXG44_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HAQ1658325.1"
                     /translation="MEIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGLGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGAVAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIATLLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYGEQPDSAHSSRIQRYH"
BASE COUNT         4723 a         2670 c         3222 g         4842 t
ORIGIN      
        1 gtatgtctta gtccctcaat aaacagtcga agagaaaata ggcaaaaatt gattactaaa
       61 gaaaaactta ctaggtcgac ggtgtagttg attttcatac ttcaacagat tcgaattaaa
      121 cgatttcgat tggcttatac cagtcctatc cgacgaaaga tatatcgttc ttaactctag
      181 cggtataact tgaagcttta cgtaaagcca atgggaatga actggagatg actttttatt
      241 atttttagta atagctgatg ggttaatact tacaactttc cttttcaaag taccatataa
      301 aaaaacggtg gggttgttta gcgaaataaa ggatctaagc caatagctta gatcctttat
      361 tctatgcttt atattatttt ttgcatgtgt gcgaaagtat actcaagccc aatcgccatt
      421 tcggaaaatc ggtactgtag agccatcttc acggattccg tcgatatcca tcttgtcaga
      481 tcctaccatg aaatctacgt gggtttgact tctattcaat ccagcttctg ctaattcttc
      541 atcggacatt tctgtaccgc ctttcacact gaaggcataa gcagaaccta gtgctaagtg
      601 attcgatgcg ttttcatcaa ataatgtatt gaaaaagatg atgccagatt gagaaatagg
      661 tgaagggtca ggtacaagtg cgacttctcc taaacgacgc gcaccttcat ctgtatcaag
      721 cagttttgct aagacatctt ctccttgttc ggcagaaaaa tcaatgactt ttccatcttt
      781 aaaagtaaat ttcatacccg agatgatggt gccagcataa ctcagtggct ttgtacttga
      841 aatgtaacca tctactcgat gactgtcagg tgctgtgaac acttcttccg tcggcatatt
      901 tgccatgaat ttttctccac gagcattata gcttccagcg ccttcccaaa gatggttttt
      961 aggcaatccg ataatgatat ctgtaccggg agcagtatag tgcagggcag aaaattgttc
     1021 tcgattcaat tcttctgctt tttttgctaa tttttcatca tgcttcttcc atgctaagac
     1081 agggtcttct tcatagacac gagtcgtttt gaaaatctga tcccataatg cctcaacttg
     1141 tttttcttct ggcagttctg ggaagacttt tgctgcccat tgtttaccag ccgcagccac
     1201 gacagtccaa ctgactttat ttgcttgagt tgccttgcgt aaattcatta gtgctttgcc
     1261 agccgctgat tgataagacg ctactcgatg gctatctaca ccagcaaatg catcgggatc
     1321 ggcagaaaca acactgatcc tgcttgctcc tttttcaagc cattcatccg cttgatcgat
     1381 tttagattgt ggtacattct caatacgatc cgttgcagca tgtaaaagaa attctctttg
     1441 gatctggtcg tcagtccatt gaacgataac ctcagcggct cctagtttat aagcttcttg
     1501 tgtgatcaat cgagcaaggg gtgcctgttc cacgctgatc tgtaaaacaa ctgtgtgtcc
     1561 tttttctgtg gctacgccag tttctgcaat taagcgtgca tattttttta gaagttcatt
     1621 aaaatcagat aacataataa aaattcctcc tcaccatttt ttctggtaac tattagcatg
     1681 ataccattta attatgattc aaacaatgag gcctttaaat aaaaaaaaga ggttattttt
     1741 ttaaggttaa gtttcatgat atatgttact acttgctatt tcagcttgtg aagaaaatca
     1801 aaaactaatt ttgcatatca aaaaaaacat gttacaatga aattaatcaa tggtggaagc
     1861 gttataaaaa aattaaggag attaaaaaaa tatggcaaga aaaaagacga ttactagaga
     1921 acagattttg gcagctgctt atgaagtagc tgccaccgat ggatttacac gatttactgc
     1981 gagaaacatc gcgaacaaaa tgaattgttc cacgcaaccg atttatcttg agtttaaaaa
     2041 tatggatgac ttgagacaag ctctttttga gcagatctat gatcatttac aatatgaagt
     2101 gttcactgtt gagcatacag ggaatacgat tatcgatctt gcttttaatt atatccattt
     2161 tgcaagaaga gagaagaagt tgtatcgttc actgtactta gaagaatatg gtggaggaaa
     2221 agagatgcag aatttctctt atacttactt tgtgaataca gtgaaacgcg atccagctta
     2281 tgtgaatctt tcagaagagc agatcgaatc tttgtataat gggacatgga tcatagctac
     2341 tgggatcgca gctttgatgt cttcaagcat catccatcca actgatgaac agattgaaaa
     2401 gatgatacaa gacagtatag atgctatctt gaaattcaaa gaaccaatag aaatcgattg
     2461 aatatactca aaagttccac aaatcagtag ataacaagag atgtgtcaaa ataaggaaaa
     2521 gttcgttttt cttacagcgt atgcattttt aactcccgaa gaactcttcc atgaatgcaa
     2581 tgaataaaag agccgtacag attgaataaa tctggatggc tcttttattg caagtatatt
     2641 aaatttttgc gactagttct ttagcgaaag cttctaaatt ttcgatatct tcttcttcag
     2701 cattcagatc cactttaaca ttttcggctc cttttgatgc gcctgttttt gcaaaagcag
     2761 catcgaaatc atccaccgat ttgcagaatt catcgtaaaa agtatcacca gatccgcaga
     2821 caccgtaaat ttttcccgat agatccaatt cttgcaagtc ttcataaaag tctacgatct
     2881 catcaggcag atcgccatca tcgtatgtat aagtagctac tacgcaaata tctgcctcct
     2941 cgaattcatc tgcatctact tgggtacatt cgttgatttc gacttcgata tccagatttt
     3001 caaatgcttc tgcaacaata tcggcgattt cttcagtgtt tcctgtcatg ctagcatata
     3061 cgatttttgc taaagccatg atgtgcctcc tcgtttataa ttatttaaca attatgaatt
     3121 ataccaaaac atcattgaaa agaaaataaa acctagagac acctggggaa tgtcatttgg
     3181 ctaaatgtat gccgctatgt taaaatgaga gggatttgta aaaaggagac gaaaatatga
     3241 ttactttaaa atcagcacgt gaaattgaaa tgatggcaga atctggagct ttattagcag
     3301 atgtacataa aaatctgcgt gattttataa aaccaggaat cacaagttgg gatattgagg
     3361 tatttgtcag aaactatatc gagagtcatg gcggaattgc tgcgcaaatt ggctttgaag
     3421 ggtatgaata tgcgacttgt atcagtataa atgatgaaat ttgtcatgga ttccctcgta
     3481 aaaagccttt gaaaaacggt gatttggtaa aagtcgatat gtgtatcgat ttgaaaggcg
     3541 gcatttctga ttcttgttgg gcctatgtag ttggagaatc tacacctgag atcgaccgat
     3601 tgatggaagt aacgaagaaa gctttgtaca tcggtattga gcaagcacaa gtagggaatc
     3661 ggattggtga catcggtcat gctatccaaa cttatgtaga aggcgaaaat ctagcgatcg
     3721 ttcgtgattt tattggtcat ggcgttggtc ctacgattca tgaagaacca gcagttcctc
     3781 attacggaga agctggaaaa gggttgcggt tgaaagaagg catggttatc acgatcgaac
     3841 ctatggttaa taccggaact tggaaaatga aaatggatcc taacggctgg actgcgtata
     3901 caagagacgg cggactttct tgccaatatg aacatacatt ggctattact aaagatggtc
     3961 caagaatttt gacatcacaa ggagaagaag gcacttattg atctatccag tcaataggta
     4021 cgggagaggt acatgaaagt ttggaacaaa ataaaacaaa atgaaaatct gatgcgcttt
     4081 attgagacga ctcaaaagcg catcactgat tcagaaatga gtacgacctc tgtcgtcgtt
     4141 gcttattatt tactcttgtc tttatttcca ttgattattg cttttggtaa tattttaccg
     4201 tttctccaca tcgatcaaga gactgtactg acctatttac gagaagtgat tcctgagacc
     4261 gtttaccagt ttatcggtcc tgcaatcaaa gaccttttga cacaaagttc aggaggcttg
     4321 ttatccgttt ctgcattagc ggctttatgg tctgcaagtc aaagtatcaa tgctttgcaa
     4381 attgcgatga ataaagcgta tggcgtagaa aatagaaaaa attttttgat cgtccgtttc
     4441 ttctcactaa ttgtcattgt cttgttcatg attgctatta gtggggtaac tatcgttctt
     4501 gggttgggac agctgatttt ggatgctatt cagccaatcg tacaaatacc cgtgaacttt
     4561 atcgatcagt tccaaacttt gaagtggcca atcacattgg tagctttatt tgtcatcatg
     4621 ttcttgattt atttaattgt ccctaatgct cagttgaagt tgaagacgat cattccgggc
     4681 acgatcttcg ctactgtcgg ctggatgctg ttatcacaag tgtttagtat ctatgctagg
     4741 tattttgcaa cgaggatcag cggttatcag atcataggaa gttttattgt actcatgctt
     4801 tggcttaatt ttgctgctac gatcatcatt ttgggcggaa tcatcaatgc agttgttcaa
     4861 gaatacgtga cgggtcatca aatcaaaaaa cgtcgagatc caagccgaaa atggttcaat
     4921 aaattgaaaa ctaaattttc tcataaaaaa aagtagaaac aagagttctt attgtaaatt
     4981 tatatcagta atttataata attatgactc ttaattaaag taggtgccca tatgcttgtt
     5041 tcttttagta tggttataag attttttctg acaattttat tatcactgat attaacacct
     5101 gtattcaaaa tcatttcagt acaaacaggt atggtcgaca aaccaaatga aagaagaata
     5161 aataaagttc caatgccttc agcgggcgga ctgcctatct ttatttcatt tgttatatcg
     5221 actttattct tattccgtaa aattatccct caatcgtata ttgtaccgat cttgattgct
     5281 tctttagtaa tcatagtcac aggagtgctc gatgataaat atgagctgac acctaaacaa
     5341 aaaagtatag ggatcttgat tgcttctctt attatttatt ttgtagcaga tatacgtatt
     5401 gattccttca cgcttccttt tattggatat atccaactag gctggcttag ttttcccgta
     5461 acgatttttt ggatcttcgg tatcacgaat gcggtcaatc tgattgacgg tttggatggg
     5521 ctggcggcag ggatttcatt gatcggttta attacgatag gaatcattgg atattttttc
     5581 ctccatgctt ctacagtgta tattccagtt gttattttct gtcttgtagc aagtattatc
     5641 ggtttttttc cttacaattt ttatccggca aagatttatt taggcgatac aggagctcta
     5701 ttcctagggt ttatgatggc cgtcctttca ctacaagggc tgaagaacgt gacttttgtt
     5761 tcatcgattt ctttgctgat cgtcatggga gtgccggtaa cagacacgtt ttttgctatc
     5821 attaggagaa aagcaaaccg tgtttctttt tctacagcag ataaaaaaca tcttcatcac
     5881 cgactaatca gtttaggctt tactcataaa ggtgctgtcc tgacgattta tgcgatggct
     5941 ttgatgtttt ctttcacagc tatggtcatg aattatactg gaagaatcgg aacgatcata
     6001 ttgattattg ccatgttgtt tactgcaatt ttattatttg aattgattgg tctgatcaat
     6061 gagaaacatc agtttatttt aaaaacattg cgatttttag ggaataaaga gtaccgtacg
     6121 caagtaaaac agaaatattt gaagaaatgg aaccgtcata tagatgaata acaaattaaa
     6181 ccagccgatc cttattagta ttgtgacggc aaacagtgat aaaatttttc ggacactaga
     6241 taattttatt tcaactgtaa aagatgaaac agcagtaaaa atccgaattt ttgacaacaa
     6301 ttcacttccc gagtacaaaa aaagactaac agagtatagg aaatatccct acatcgatat
     6361 ctattttcat gatgaaaatt ccggatttgg ttatggacat aatcacaatc tgatagctag
     6421 cgattctcgc tatggcatta tttgtaaccc agatatttta gtggatgaag agacagttac
     6481 agcattggtc ttgttattga aagaacatcc ggaatgtgcg atgttagcac ctaagattct
     6541 taatgaagat ggaacaactc agcacctcat ccgtgagcag ctggctgttt ttgattacgt
     6601 tttgcgcttt gtgcctttca aatgggtcaa aaaaaagttt gacaaacgac tggctgtgtt
     6661 tgaatgccga ggattgccta ctgatgctaa tagttatgta cggatgatct caggtagttt
     6721 tatggtagct gatattgata aatttaaaga gataaagggg tttgatgagc gtttcttcat
     6781 gtactttgaa gacaacgact tgtgtttgag ttttgaaaaa gcaggttaca agttattgta
     6841 cacacccttt tatgcagtta tccattttta cgggaagggt gcgcatcgaa atgcaaaact
     6901 gtttcgcgtg tttttgactt ctatgatgaa atttttcaat aagtgggggt ggcgtttttt
     6961 ctgatgaaac cagaaatatt agtagtcgtt gtgctttatc agcaactatt cagtcaatct
     7021 ccagcttatg gaccattaag caaagcactt agcgaaaagc aaattcagct tattatttac
     7081 gataatagtc ctcaaaggca gcaaaatgag ctgtttgaaa aagaaaatat cgtttatcat
     7141 catgatccgc aaaatcctgg tttagcggtg gcttataatt ttgcgttgtc tcaagcagat
     7201 gaaggaacac gttgtctcgt cactctggat caggatacga ggctagtcga cgattatttt
     7261 gaactattgc gtaaagtaac ctttacagat gaatgtgttg ctgcagttcc gatgattttt
     7321 tctgggagcc gacaagtttc tcccctatat tcagatcgtt atatcaatcg tacagctcaa
     7381 gcagtagaag ttgaaggccc aacagctgaa cgaatcatgg caatcaattc aggtgttgcc
     7441 tggtctgtca aatttctaaa aaagattggc gggtttaatc ctgctttttc tttagatttt
     7501 ttggatcatt ggctgttttg gaaagtggat cagttggaaa aaactgtgga gatccttcct
     7561 gtacgaatgg aacatgatct ttcggtttta gactatgaaa aagtttcaag taatcgatac
     7621 cgttctattt tacaagcaga aaatcgattt tatcatgaat atgatcaaag gcatttatcc
     7681 agtcaccgac gacaattatt gttaagaaca gctaaacagt tcttgaccgt caaaaaccgg
     7741 catatctggc ggatgacttt gcgctcatat gttaagaatt ggaaggtgtg aattcgtgcg
     7801 ttcggtttgt atagctacct ttaatggagg cagatattta agagagcagt tagatagtat
     7861 tttatctcag ctttcagcag aagatgaagt agtcatttct gatgatgggt cagtagatga
     7921 tacatgggat atattaactg aatacgcaga aaaagataag cgaatccagc ttttttcggg
     7981 gcctcgtcaa ggattgatcg ctaattttgg ttatgcgatc cagcagacga aaggagatct
     8041 aatctttttg gcagaccaag atgatgtttg gttggagaca aaagtgaaaa agatcactga
     8101 ctattttgag cagaatccgg atcagctagt agtagtttct gatttaatca ttgtggacag
     8161 cgagctgcaa gagatccatg attcgtattt ttcgtttcga aattccaaag aaggatggct
     8221 taataatgtg ataagaagtc actatattgg tgctggtatg gctttccggt cttctttaaa
     8281 acccgatatt ttgccgatcc cgtcagaagt gccgatgcat gatatgtgga ttggtttgct
     8341 gggaggaaag aagactgggt tcctaagaga accgttaact ctgtaccgaa gacatgattt
     8401 aaacgcgagt gagattgaaa cacattctaa attacggcaa aaaatttctt ggcgtgttca
     8461 tctgattacg gcgttgatca aacgaaaatg gtttaaacgt tgataaaaga tgtttaaatc
     8521 cttaatattt ccatgacaaa tataacaatt gatgagataa taacaaagtg aaatttgaaa
     8581 tcaaaaagga gagcagatat ccatgaaggg aatcattctt gctgggggca gcggaacacg
     8641 tctgtaccct ttaacaaagg caacatcgaa acaattgatg ccgatttatg acaaaccaat
     8701 gatttattat ccaatgtcta ctctgatgtt ggcgggaatc aaagaaattc tgattatttc
     8761 aacgccacaa gatacaccac gtttcaagga attatttgga aacggaaatg atttaggact
     8821 gcagatcgaa tatgcagtac aagaaagtcc agatggattg gcacaagcgt ttatcattgg
     8881 agaagaattt attggagatg atagtgtctg cttggtatta ggggacaata tttactatgg
     8941 tggcggtctg tctaaaatgt tgcaacgagc agcttcaaaa gatacaggcg cgacagtatt
     9001 tggctatcat gtcaatgatc cagaacgctt tggtgtagta gaatttgatg agaatatgca
     9061 agctttgtcg attgaagaaa agcctgctca gccaaaatca aattacgctg taactggcct
     9121 ttatttttat gacaatgagg taatttctat cgctaaaggt atcaaacctt cagaacgtgg
     9181 agaactagag attacggatg tcaataaagc atatttggat aaaggaaaac tatccgtaga
     9241 acttatggga cgcggatttg cttggcttga tacaggaaca catgaatcat tgcttgaagc
     9301 atctaccttt atcgaaacaa ttgaaaaacg tcagaactta aaagtggctt gtttggaaga
     9361 aattgcttac cgtatgggct acatcgataa ggaacaattg ctggcccttg cacagccatt
     9421 gaaaaaaaat caatacggtc aatatttatt gaatttggcc gctgaataag gagtccttac
     9481 agatgaaagt aacagataca aaattaacag atgtcaaaat cgtagaaatg gacgtctttg
     9541 gcgatcatcg cggtttcttc acagaaagtt attcaaaagc aaaatttgca gaacatggat
     9601 tgaaatatga ctttgttcaa gacaaccatt cactatcagc tgaagctggt gtccttcggg
     9661 ggcttcattt ccaaaaagga gaagctgcac agacaaaatt gattcgggta gtcactggag
     9721 cagttttaga tgtagtagtg gatatccgca aaggtagccc gacttatggt caatgggacg
     9781 gatttatctt atctgaacat aatcatcgac agttgcttgt acctaaagga tatgcccatg
     9841 gattcgttac tttgacacca aatgtgaact tcctttataa gtgtgataat tactataatg
     9901 cagaagcaga tgggggcatc gcatttgatg atccagattt agcgattgat tggccaattg
     9961 atccatcaag agcgattatg tcggaaaaag atcaccgtca tccgacatta aaagaatttg
    10021 aagcagaaaa tccatttgtt tacggagaaa tctaagaatt aaggagaaat tttcttatga
    10081 aaaacatcat tgttaccgga ggtgccggat tcatcggctc taatttcgtt cattacgtgg
    10141 taaacaatca tccagaagtg catgtcactg ttttggataa actgacttat gcaggaaaca
    10201 aagagaactt ggctggactt cctagcgatc gcgtagaatt ggtcgtagga gatgttgcag
    10261 atgcagaatt agtggaccgt ctagtaaaag aagcggacgc agttgttcat tacgcggcag
    10321 aatcccataa cgataattca ttaaaagatc ctttcccatt tgtacaaacg aatatcattg
    10381 gtacgtatac acttttagaa gcttgccgca agtatgatgt acgctatcat cacgtatcta
    10441 ctgacgaggt ttacggcgat cttccattac gtgaagacct gccaggacat ggggaaggcg
    10501 aaggagaaaa attcactgcg gaaacaccat ataatccttc aagtccatat tcttctacaa
    10561 aagctggttc agatctgtta gtaaaagcat gggtgcgttc ttttggctta cgtgcaacca
    10621 tttcaaactg ttcaaacaac tatggtcctt atcagcatat tgaaaaattt attcctcgcc
    10681 aagtaacgaa tatcttaagc ggtattcgtc caaaattata tggcgaagga aagaatgttc
    10741 gtgactggat ccatacgaat gatcattctt cagcagtgtg gctgattttg acgaaaggca
    10801 gaattggcga aacctatctg atcggtgcag acggtgaaga agataataag accgtgatgg
    10861 aattgatctt agaaatgatg ggtcaaccaa aagatgcgta cgaccatgta aacgaccgtg
    10921 caggacacga cttgcgttat gcaatcgatt cgacaaaatt acgggaagag ttaggatgga
    10981 agcctgagtt tacgaacttc cgtgatgggt tggctgacac gatcaaatgg tacgaagaac
    11041 atgaagactg gtggaaaaaa gaaaaagagg ccgttgaagc agcatatgcc aaaaatggac
    11101 aataatacag agaaaatgtc actgtattaa tggaaaaaca catgtgaggc aggcgctcgc
    11161 ctgtctcttt tttaaaataa aaatcaagga gcggataatc atatgttttt attaactgga
    11221 ggaaatgggc aactaggtac agaattgcgc catttattag atgaaaaaaa tctgcaatat
    11281 gtttcaacag atgcaaaaga cttggatatc acagatgccg agaaaacaat ggcttacatc
    11341 acagaattaa aacctgatgt tattttccat tgtgcagctt acacagcagt agataaagca
    11401 gaagaagaag caaaagaatt agatgagaag atcaatgttg atggtacaag aaatgtagca
    11461 cttgcagcaa aagctgtgaa tgctcaattc gtctatatca gtacagacta tgttttcgat
    11521 ggaaaaaaca aaaatgtaga atacgaagta gacgaccaaa cgaatccact aaatgaatat
    11581 ggtcgtacga aattattagg cgaaaaagca gtagaagaga ttttagaaga ctattatatc
    11641 atacgaactt cttgggtttt cgggatctat ggacacaatt tcgtcttcac gatgcaacgc
    11701 ctagcggaaa cccgcgacaa actgactgta gtaaatgacc agtttgggcg accaacttgg
    11761 acacgtactt tggcagaatt tatggtatat gtcatcgaag aaaaagcacc atttggtatt
    11821 tatcatttgt cgaacgaaaa tagctgttct tggtatgaat tcgcaaaaga aatattgaaa
    11881 gatacagatg tagaagttgc acctgttacg tcagaagaat ttccgcaaaa agcaacacgt
    11941 ccgcagtatt cagtcatgag cctgaaaaag acagaagagt taggatttgt cataccaact
    12001 tggcaagaag cacttgcgca aatgctggaa gctgccaaaa aaatgaataa gtaatttcct
    12061 tttatcaaat agaatttgga gcatgatatg gaaaaacaaa caagaagtgc acgacgaaaa
    12121 gaaaagccaa aagcgaacca ggcagattcg aatttggtgc ttgacctgtt tctaaaactc
    12181 cgctatctta ttggcgtttt agtcatcgtg gtcatcgtta cttttaattt aaacggaagt
    12241 tcacttggtg tttgggataa atatgtgtcg caaagagacg atgggaaaaa aactgatgtg
    12301 atttttggac agaatcggga agtgcggtca gacgaatggc tggtgcagac acctttttac
    12361 ctctcacaag ctgaagagga ctttccgttg gtgaacgagg attattcatt aaacggacaa
    12421 aacatgatca ttgcgtataa ttctcctgca aaggatatta cggttatcgg aaaaccattc
    12481 aactggggat tctttttcct cggaagagat cgaggacttt ccttttattg ggcgatgaaa
    12541 ttagtcgtca tggtcttgct tggattcgaa gtactgatga tattaacgaa gagaaaaaaa
    12601 gctctatcgc taatcggtgc atttgcgctg acattttctc cagcagtcca gtggtggttc
    12661 atgcaacatg tgggcgattt gattttcttt actttgggac tgatgactgc tttttatcat
    12721 tatttctaca atcatgataa aaaatggcta cgaacgttga tgatgctttt ggtggttatt
    12781 ttcggtattg ggtttatttt agtgatttac ccagcccatc aagtgatgct ggcgtacttg
    12841 ctgatttttt attttgttgg cctgcttatt tactatagca agaaagtttc atgggatctg
    12901 ttggatgtag tgttgattgg aggggcagtt ttatttattg gcggagtcat ggttcatttc
    12961 tggctaacgt caaaagacgc attgttagct tctttgaata ccttatatcc aggaaaacgg
    13021 gtaagtactg gaggaaagtg gacgattggc gaattttttt ctttccttac aaattggaaa
    13081 attccttttg aagatatcaa ctattcgaat aactctgaag tggccctgtt ctatcacttt
    13141 tttccaactg tattcctagc aagtccattt gtgttatttg gcaaaaaaga tagcgaacaa
    13201 aagttactgg gacgtatttt gatgattttt tgtctgtttt ctatcttttg gattaccgtt
    13261 gggttgccaa aatggctagc agaaatcact ctgctgtctt atgttcctcc attaagagcc
    13321 atccttactt ttagttttgc ggcttgttta ttatcgatct ggtttatcgc ttatatttgg
    13381 agagaagatg ttattcctat ttggtataaa ttcattttgt tggcagtaaa tgcatggtct
    13441 tatttctatg cgattacgca ttcgaaaata gataattatt tttcggattt tgaaaccatc
    13501 gcagttgctg tgttgggaac agtgatttta gcatttgcat tatttggctg gagacgcttg
    13561 ttttacctcg cttttgccgc cttagttatc gtatctggct ttttcgtcaa tccagtcgta
    13621 gaaggtacag gagcgatttt tgaaaaaaca ctggcaaaag aaatccaaaa aatcgatcaa
    13681 caagatcctg gtaaagtttg gttgtctgaa gatgaattat ataacttcac cccaacattg
    13741 ggagtgaaat ccttcaatac agtacgcttt tatccagata tagctgcatg gaaggaaatc
    13801 gatccgaaag aagaatacga gaagtattat aatcggtatg ctcatacaag agcttttatc
    13861 gctaaggaag atacaaagtt cattttagat cgtccagata tgttttcggt cacgttgaat
    13921 tttgaagacg cagaaaaatt agggatcgac tatgtgatca gtaaacgacc attaactgat
    13981 tacaaccaat tatatgaagc gcagttcacg cagctatatg gaccggataa agacggttat
    14041 atgattttta aagtagaata tccggattat ccagatgtac cacaaacaca aactcaggaa
    14101 aatcagctag tcgtacagta ataggctagc tgagaataat cctgaaatat tagtagaaag
    14161 gagagtggag ataattcaag caatcgtaca aaaatttgtc aacaaaatga gtgtcggttt
    14221 cttgcttttg ttgctgacgc tagtcttttt tgtttggaca gcagggttag gttttccagc
    14281 aactttgttt atatataaca atagttcatt acttatcgct tttagtgtgg ctattttgct
    14341 cattttaaat gtttataaag caaaaggcgt cgattttctt tatctaggtg ttgcgcttgc
    14401 ccttttcatt ttcttttctt actttcggga attaagaaat gagtctttat ttggagattt
    14461 actgtttcct ttgattattt tattttgttt agtaattaaa tggatcgagt ggacaaaatg
    14521 ggatcgcggt atttatctga ttgtctttac ggtcttcctc ggaatcacgt tatatcgtgt
    14581 atttacagag atccaagtac cagaaggaca aagcatctgg gctccaggca attcgctttc
    14641 tgatatttgg atcaatacga atacaatcgg aagttctttg atgactcttg ctttattgat
    14701 cagtggattt gccagttctt ttgaacgatg gtatgtacga atcatcggtg cagtagcagt
    14761 tgttgcagct tttctcggga tttgggtctg ccaatctaga ggtgcattag tcgcgttgat
    14821 tgtttttgcg atattggatc ttttacctaa atcgttgatg cgtgtcattc gtgcgccatt
    14881 tattgcttac acggttacga ttcttttagc tttgccgatt tcttatctgg cggctgtctc
    14941 tgaaaaagtc aatttgttta ccggtcgaga agatatttgg cataaattct atcagacgct
    15001 aggagaaaaa tctgaacaag ttttactagg aatgaaaaca tttatcttcc aacgaggaaa
    15061 ccaattttta ggaaaccaca atagttataa ttctattttg aatatctatg gattgatagg
    15121 atttgggatt gctactttgc tgcttatcct ttttattggt cgcttaacgt tgaaagcaga
    15181 tttatccaac gggcagatga cttttatctg ggcctttttt gcggtgatga tgcagtcatt
    15241 tatggaggat acattgactt catacccatg ggttccgatt atttatatcc tgttggctat
    15301 ggccgttcat cgatatgatg aaccagaagt tgcggaggaa ttctatgtta tggaagaggc
    15361 atatgacgat atgacttatg atgatacatc ttatggggaa caaccagata gcgctcattc
    15421 ttccaggatc caacgctatc attgataggc acaaaat
//
LOCUS       DACEUZ010000062        15390 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.062, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000062 DACEUZ010000000
VERSION     DACEUZ010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15390
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.062"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(12..482)
                     /locus_tag="IXG44_001603"
     CDS             complement(12..482)
                     /locus_tag="IXG44_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HAQ1658326.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            complement(641..1099)
                     /locus_tag="IXG44_001604"
     CDS             complement(641..1099)
                     /locus_tag="IXG44_001604"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HAQ1658327.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            complement(1270..2415)
                     /locus_tag="IXG44_001605"
     CDS             complement(1270..2415)
                     /locus_tag="IXG44_001605"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HAQ1658328.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            complement(2428..3345)
                     /locus_tag="IXG44_001606"
     CDS             complement(2428..3345)
                     /locus_tag="IXG44_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HAQ1658329.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPNGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            3773..4336
                     /gene="efp"
                     /locus_tag="IXG44_001607"
     CDS             3773..4336
                     /gene="efp"
                     /locus_tag="IXG44_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HAQ1658330.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            4822..6081
                     /gene="lysA"
                     /locus_tag="IXG44_001608"
     CDS             4822..6081
                     /gene="lysA"
                     /locus_tag="IXG44_001608"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HAQ1658331.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            6068..6868
                     /gene="dapF"
                     /locus_tag="IXG44_001609"
     CDS             6068..6868
                     /gene="dapF"
                     /locus_tag="IXG44_001609"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HAQ1658332.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            6871..7752
                     /locus_tag="IXG44_001610"
     CDS             6871..7752
                     /locus_tag="IXG44_001610"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HAQ1658333.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            7749..8465
                     /locus_tag="IXG44_001611"
     CDS             7749..8465
                     /locus_tag="IXG44_001611"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HAQ1658334.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            8471..9166
                     /gene="dapD"
                     /locus_tag="IXG44_001612"
     CDS             8471..9166
                     /gene="dapD"
                     /locus_tag="IXG44_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HAQ1658335.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            9182..10183
                     /locus_tag="IXG44_001613"
     CDS             9182..10183
                     /locus_tag="IXG44_001613"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAQ1658336.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            10180..11541
                     /locus_tag="IXG44_001614"
     CDS             10180..11541
                     /locus_tag="IXG44_001614"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HAQ1658337.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            11501..12652
                     /locus_tag="IXG44_001615"
     CDS             11501..12652
                     /locus_tag="IXG44_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAQ1658338.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            12627..13796
                     /locus_tag="IXG44_001616"
     CDS             12627..13796
                     /locus_tag="IXG44_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ1658339.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            13971..15350
                     /locus_tag="IXG44_001617"
     CDS             13971..15350
                     /locus_tag="IXG44_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ1658340.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
BASE COUNT         5020 a         2719 c         3282 g         4369 t
ORIGIN      
        1 tttttattaa gttaaagaac ggattcaatt gcagcggtca ttttcaatgg agaagtttgt
       61 ggtgcaaatc gttcgattac tttgccattt ctatcaatca aaaatttagt aaaattccat
      121 tttattgctt tagtgacagt accaggagct tcatttttaa gataagtaaa aagaggatct
      181 gtattttctc catttacgtc aatcaattga tgcataggga aggtaacgcc ataagtggtt
      241 ttacaagctt ctgctgcttc agtcgctgaa tctagctctt gtttaaattg attggaagga
      301 aagcctaaga taaccagacc ttgtgtctga taagcttggt aaagttcttc taattgttta
      361 aattgaggtg ctagaccgca ttttgttgca gtgttaacaa tgattatcgg tttgcctgca
      421 tattttttca atgaatagac agtgccgtct tctaaagtag tttcaaaatt ataaatagac
      481 ataaagtttt ctcctttcta ttttatataa taaaaagagc gctaaactga aaactagcta
      541 tttgttttcc cgtttagaac tcttttcaga caataaatat ttaataaaat acgaagataa
      601 gatagctata ttgcttctca tctttacgtt cttttagaaa ctatgcttct acttcagacc
      661 attcatcgcg ttttgctaaa aattcacgag ccaaagcttg tgcaccttct agtgtatggc
      721 tggcagccca gccacattgt gtttcattgc ttgcaggtac ttcagtagct tcaagaacat
      781 ctttcattgt tgcttcaaga acatctaaaa tgtcttcgta gctttcatga ttcaatacag
      841 tcaagtaaaa gcctgtttgg catcccattg gagaccagtc tacgatgtaa tctgcgtgat
      901 tgcggattag ttcggcagtc aagtgctcaa gtgaatgtag acttggcatg tccatgtgtt
      961 ctttatttgg ttgtttaaag cggacgtcgt atttaatgat gacatcgcca tgcgcacccg
     1021 tttttcgatc agctacacgt acataaggtg ctttgacttt tgtatggtca agattaaaac
     1081 tttcgacatt cattctcatt tgtaagactc ctatctgttt gttcattcgt tttgatgctt
     1141 ttcactaaag aattatttag tgaaatttac aaaaacgtct ttctacttga ttctaacact
     1201 tatcgtttcc aaggtgtagc aatctgaaat attttttaga tttttctgct aagtcgttta
     1261 ttgggacttc tagttcttga ttctgtcaaa tgcctgagct aaatcgttta tgagatcctg
     1321 accatactca attcctacag aaagacggat caagccgtcc tttatgccag cctcttctcg
     1381 tttttctttt ggaatagacg catgagtcat gacagaagga atttccacta aactttctac
     1441 gccacctaaa ctttctgcta aagtaaatag ttggagtgat tcaacaaatg gaatagcaga
     1501 ttcttcattt tttaaagtaa aagaaatcat tccgctaaag ccgctcatct gctgcttagc
     1561 aactccatgg agttcatgat cgggtaatcc tggatagtag accttttcta ctgctggatg
     1621 agaaaataga aaatcagcta ccacaaaagc atttttttga tgttcctcca tacgaacact
     1681 tagtgtcttg attccgcgct gaagtaacca gctatcttgg ggaccaagta cggctccgat
     1741 agcattttgg taaaatccga tctgatcggc tagatcttta tggttagaag tcactaaacc
     1801 agctactaca tcgctgtgtc cgcctagata tttggtaccg ctatggacaa cgatatcaga
     1861 accgagatct aaaggtcttt gaaaataagg agtagcaaat gtattgtcag caataactat
     1921 caatccatgt tgatgagcca gagtggcact tttttctaaa tcagtaatct ttagaagcgg
     1981 attgctaggt gtttcaagat aaagtgcttt tgtattgggt ttgatggatt gttctatttt
     2041 atcaagatta ctcgtatcta tgatcgtgta ctcgagacca tttttagtaa gaactttgtc
     2101 aaatagacgg aatgttcctc catagacatc atctcctaat aagatatgat cgccagcttg
     2161 aaaaagagaa aaaacggcat gaatcccaga aagaccagaa gaaaaagcga atcctcgaac
     2221 accgccttcc aaatcagcaa tcagttcttc caatgcaaaa cgagtaggat taccagatct
     2281 tgaatattca tattgtttag gctggccgac gccatcttgc cgataagtgg atgtttgata
     2341 aataggtaca ctgacagctc ctgttgtggg atcttcactg atgcctccgt ggatcaattt
     2401 tgtttgaatg tgcatgtttc tttcctccta taagtaaatg ttttttgata aatagcggtc
     2461 tgctgcgtct ggaaaaatcg taacgacacg atgaccaggt ggtaaacgac gaatttctct
     2521 taatgctgct gcaaaagccg caccactcga acttccaact aatagtcctt gttctcgtgc
     2581 taactgccta gtatagttaa acccttctac atcagagatt gtctcgatct gattgattga
     2641 aaggggagaa agaaacggcg gaatgaattc tacaccgata ccctcaattt cgtgaggggc
     2701 tggatcacca ccatttaaca cagagccctc tggttctaca ccgattattc gaatgtcggg
     2761 atatttttct ttcaaaaagg tagaagttcc agcaaaggtt ccaccactcc caattcctgc
     2821 tacaaaacta tggatatttt ctttgatttc atgaaagatt tctggcccta acgtatgata
     2881 gtaagcagca ggattatctt tattttcaaa ttgaagaggc agatagctgt ttgatatctc
     2941 ctctgcaagt tttttacttt tgctgatggc accgatgatt ccttgctcac taggagaatg
     3001 gatgatcttt gctcccaatg ctttcatcaa ttgttgtttt tctaagctaa acttctctgg
     3061 aacaacaaaa accgttctta atttgtatgt gagtgcggcc agagcgatcc caattcctgt
     3121 atttccggca gttggttcga tgatcgtcgt tttttcattg atttttcctt gggcaaatcc
     3181 ttctttgatc aaataactgc ccaatctatc tttgatactt cctccaggat ttaaatattc
     3241 tagttttgca taaatagcag aaccattagg catagaatag tgctgattgt caaagcgata
     3301 aagcggtgtg gaaccaatag cttctgtaat tgtcgagata atcatgtggg cctcctaaaa
     3361 aaagataata aaaaaaggag ctgactatta aatcagctcc ttgaaagaca tagagaaaaa
     3421 ttgagtagat aaaaagataa gttacctggt actcattcta tgaagggcaa ttaatagaca
     3481 tgacaaatag tatgctgctt ttatgcacca tacaacagca acgattcaat tgtttttggt
     3541 tgctgactgt catgtgtatc cctccaagta aaatttttat taacgatagc atgaaaaaaa
     3601 ataaaagtca aaggcttgaa agaaaaaaat atttcgatag catcgtttac ccgtgtttat
     3661 ttttctgaca aataggcatg agaccttaaa agatagagaa tactctagtt ttctggccgg
     3721 ttgtctgata caataaagtt gattatgata aagaaaagaa ggagcaaaaa taatgatctc
     3781 agcaagtgat ttaagagccg gtatgacttt cgtacaagac ggcaaactga ttaaagttct
     3841 ggatgcaagc caccataaac ctggtaaagg aaatacagtt atgcgtatga aactgaaaga
     3901 tgtccgttca ggtgcaacat acgacacaac gttcagacca gaagaaaaat ttgaaaaagc
     3961 acatatcgac acaaaacctg tacaatattt gtacacgatg gatgatacag ctttcttcat
     4021 ggatttggaa acatatgagc aatatgaaat tcctgtagaa acagtagaag aagaaatgaa
     4081 atatatccta gaaaacatgg aagtaaaaat ccaatttttc ggtagcgaag tcatcggcgt
     4141 tcaattacca acaactgtcg tattacgtgt tgtggaaaca caaccttcca tcaaaggtgc
     4201 aacagtcact ggttcaggta agccagcgac aatggaaact ggcttagtag tcaatgttcc
     4261 tgacttcatc gaaacagatg aattgttgga aatcaataca gcagaaggtt cttatgtaaa
     4321 acgtgcgtca aaataattcg ctaacggatt gataagaatt tgaaaacaga gcttgacctc
     4381 ttgaaaggca gatcatcgga tctgcctttc aagaggtttt ttgtggaatc agcaaattga
     4441 aaaataaaaa agtatttgat tttatttata ttttgaaaga tgaatggcaa aagctagaga
     4501 aagaaaaaac tgcacttatt tatgaagaaa ttgaaatgat tcataaaatt gaaaatatcc
     4561 gaactattcc gaactatatt gaagcgaaaa tattttttca tcactaaata gtgttgaatt
     4621 tatgttttta tgtataaata gcttctaata gttcgggtat tttgttttta taaccatttt
     4681 tattcttatt tttaaaaaaa tatttcgaaa tatactagac aattcagaaa attttgttta
     4741 atattattcc aataaaattc aaatctttat caaatgaaat tccaataaaa acggaaaaaa
     4801 agtgaaaaga ggaatgaaaa aatgacagaa ttgataattg gcggagtcac agcaagcaca
     4861 ttggcagaaa agtataagac acctctgtat gtgtatgatg aagaaaagat gagacaaacg
     4921 atgctgtcat tcaagcgagg ctttgtatcg aaacgttttg atacaaaagt gctttacgcc
     4981 tctaaagcat tccaaacgat tgagatgctg catttagttc gtgaatatgg attcgggcta
     5041 gatgttgtca gtggtgggga aatctatacc gctttgcaag ctgattttcc tagagatact
     5101 atctacttcc atggtaacaa caagacgcca gaggaactga tctatgcact tgaaaatgat
     5161 ttgttgcata ttgttgccga taatttgatg gaagttgaat tattagcaga attaagccaa
     5221 cgctatttaa aaaaaatttg tatcatgctt cggttgaatg tcggagtaga agcacatact
     5281 catgaatata tcgtcactgc gcatattgat tcaaaattcg gcatgagcta tgaaagtaaa
     5341 gagtgccaac aagcccttca gttgataaaa gaaagcagct atctagagtt agaaggattt
     5401 catgctcata tcgggtcaca aatttttgat gttactgctt ggttagctga aatcgacaaa
     5461 ttgacgaatt acttaaccga ttttgaccaa cctctttcct tgaatcttgg cggagggttt
     5521 gggattcgtt atacacaaaa tgatcagccg atcccagtcg aagaggcact aaaaaaactg
     5581 attgaatata cggaagaagc tttggaaaaa cgaaaactga ccattcgtca attactgatc
     5641 gaaccgggta gaagtattgt tggagaagct ggtacaacac tatacacgat tggtttcatc
     5701 aaaaagacac cacataaaaa atattacttt gtagatggcg ggatgacaga taatatccgt
     5761 ccagctctgt atcaggcaga atacgattgt gatttagcag caaaactgtc ggaagaaaaa
     5821 acagagaaag tgacgattgc aggaaaaatg tgtgaatcag gcgatgtggt aattaaagaa
     5881 gcttatttgc cgaaagcaga atcgggtgat cttttagctg tttattccac gggagcttat
     5941 ggatactcga tgagcagcaa ctacaatcga gcgacacgac cagcagttgt ttttgtcaat
     6001 aatggccaat caagactgat tgttcgcaga caagcatttt ctgatctact aaggggggaa
     6061 gtttcctatg atccttcata aatataatgg ctgcggaaat gattttatct tattagatta
     6121 cgcagaagac acagattata gtcaattagc cgcttcttta tgcacaaaag aaaaatatga
     6181 tatagacgga ttgatcgccg taaaaatcga tccgttggaa atgatctatt acaacaaaga
     6241 tggcagccgc gcgccgatgt gcggaaatgg cattcgctgt tttgctcgat acgtaaatga
     6301 tcagaacatg atgaatgaac aggaattcaa cgtagagaca ttagcaggca caagaaaaat
     6361 tacggtgttg gatccctcac ctttttattg cagcgtagat atgggagtgc cagatgattc
     6421 tccggaaaaa atcaaagcta atcaaactta tccaataaaa aaccaggaaa tagagatcaa
     6481 tggtgaaaaa gtaacgatca ctagtttatt tttaggaacg attcataccg ttgtttttgt
     6541 tgaaaatgcg cgtgaggaag tctggagaaa aagaggtgaa ctgatctgtc atcatccttt
     6601 gttcaaagaa aaaaccaatg tcaactttgt agaagtagtc aatgaacaag aattgatcgt
     6661 ccgtacctat gaaagaggag taggttggac acttgcttgc ggtacaggtt gctgtgcctc
     6721 ttatgtggtt gctaaggaaa ctgggaaaat ttcagcagat caggtagttg ttcacttgga
     6781 acaaggagac ttgatgatct ctggtgagga tgcgatacaa atggctggcc cagcagttca
     6841 tgaatggaca agaatattgg aggaatgaag atgataaaag gttcgatcgt ggcgctgatc
     6901 acgccgatga atgaagaggg atcagtagat tatgctggtt tggaaaaatt gatccaattc
     6961 catttagatg aacaaacaga tggattatta gttttaggta caacaggaga atcttctacg
     7021 ctcacacaat ctgaggagga gcagatcctt caactgacag taaagaaagt ggctggccgt
     7081 gtgccggtga ttgcaggagc tggaacgaat aatacgaaag aaacgattga aaaagcgaag
     7141 cattttgctt ctttgggggc agacgcactt ttggtcatca ccccttacta caacaaaaca
     7201 agtgatgcag gactagctgc tcattttaca gcaattgcag aagcctcacc gattccgctt
     7261 attttgtata atgttccttc tagaacaggc atgtcgattc caatccatgt attagtaaat
     7321 ttagcagaac atcctaatat cattggtttg aaagaagcct caggagatat ggcttatgtg
     7381 atggatgccg ctcggttgat tggagaagaa ttcttccttt acagtggaaa tgatgatttg
     7441 atcctaccag taatgagtgt aggaggaagc ggtgtaataa gtgtatgggc gaatatccaa
     7501 cctaaaatag tccatgaatt agtaaaagat acgcaggacg gaagatggca gcaagcaaaa
     7561 gaaaagcaat taaatgcact tgaactgatc catgccttgt tcagtgagac caatcctatt
     7621 cctgtcaaag cagccatgtc attattagac ttaccttccg gacctctacg cctgccttta
     7681 gtttctcttt caaaagagaa aaagaaacaa ttagctcagc ttttgttaaa agaaaggacg
     7741 gttgaaaaat gaaaatcgga ctgattggca gtggacagat gggaaataga gtcgaagaag
     7801 ttgcacaaga aagaggagat acggtccttc ttttacggac ggcacctaaa gaagcaacta
     7861 cgcttgcttt tacggaactg ccagaactgc ttattgattt ttctcatcca gataatttag
     7921 agaaaatcct ttcgtatagc ttatcatatc aacttccgtt agtcattggc acgacaggtt
     7981 atacacaatc acagttccaa gaaataaaag aacatgccaa acatgtacct gtgatgtata
     8041 gtgctaattt ttcgttcggc atcatggtga tgaatcaact aatcaagcaa gcagctgcaa
     8101 tgttgcaagg ctggcagatc gaactgattg aaaaacatca tagtcgtaaa aaagatgcac
     8161 catctggtac tgcaaatatg ctgcttcaaa caatccaaga agtgcaaaag ctgcagcccg
     8221 tatacaactg gcaagggaaa aaaagagaag aaaaccaaat tggtgtacac agtattcgtg
     8281 caggttcttt acctggtgaa catgatgtat tgtttgctgc tactgatgaa atatttacga
     8341 tcaaacatga atctttttct aatcgaatct ttgctgcagg cgcagtagaa gcagcggact
     8401 ggttgaaaga tcagtcacca ggttattaca aattagaaga tttattaatg gacaaagggg
     8461 tgtaaaaaac gtggaagctc aagaaatcat ccgctacatt caaacggcat ctaaaaagac
     8521 gcctgtgaaa gtctatctga atgtaaccga gccaatcgaa tttaaagaaa gtaaagtttt
     8581 tggtgaagga agcagcttcg ttgtttttgg tgactatgag acgattgctc cggttttaga
     8641 agctgaatca gaaaaaatca ttgaaatcga aatcgaaact gctgcaagat acagcgcagt
     8701 tcctttgcta gatattaaaa aaattaatgc tcgtatcgaa ccaggtgcta ttatcagaga
     8761 tcaagtgtca atcggcaaca atgccgtgat catgatggga gcgattatca atattggtgc
     8821 agtaatcggt gaaaacacga tgattgatat gggtgctgtc ttaggtggac gagcgacagt
     8881 gggcaaaaac tgccatattg gtgcaggagc tgtcttagca ggtgtgatcg aaccagcatc
     8941 ggccaaaccg gttatcgtag aagacggcgt actggtaggt gcgaatgcag tgattgttga
     9001 aggtgtccat atcggaaaag atgcagtcgt ggcagcagga gcagtggttt tagaagatgt
     9061 ggcagcagaa actgttgtcg gtggaatacc tgcacgtgta ttaaaaataa gtgatgatca
     9121 gacaaaagaa aatacagcat tgattgctgc gttacgcgaa ttatagagga gatggagaaa
     9181 aatgcgcgaa atagatacct gtattgtggg ggcaaccgga ttagtcggcc aaacgatgct
     9241 caaagtgtta gaagaatatg attttccagt aggacgattg aaattattag cttcaaaacg
     9301 ttcagccggt aaagtttgtg tattcaagaa aaagaaacac actgtacagg aattgacaga
     9361 aacttcattt gaaggatatg atttagcgct attttctgca ggtgcttctg tatccaaaga
     9421 gtttgcgcca atcgcacgaa gtcagggact atttgtcata gataattcct cagcatggcg
     9481 ggaagattcg gatattccat taatcgttcc agaggtcaat ctggaagact atacattgaa
     9541 tcggctgatt gcaaatccaa attgttcaac cattcaagcg gtacttccgc ttaaagcttt
     9601 acaagaagcc tttggtatcc agcgagtcaa ttattcgacc tatcaagcag tttctggttc
     9661 cgggcaaaaa ggcattgaag atcttcatcg tacgcaaaat gacgaggaac cagttttgta
     9721 tccttataat attagccaaa cggtgattcc ggaaatcgat cagcctttgg aaaatgggta
     9781 tacaaaagaa gaacagaaaa tgataaatga gacccgtaag attctgcatc agcctgcttt
     9841 gccagtatca gcaacctgcg tacgtgtgcc aatcgaaaat ggacatggag tctctgtggc
     9901 agttcaactg caaaaagagt ttacgacgga agaaattaga gagtgtctca gccagtttcc
     9961 agggattcaa ttagaagatg atctagcagc acataaatat ccgacgagca ttcttgctag
    10021 aggaacagat atggtttatg ttgggaggat cagaaaagac acttctttag agaacggatt
    10081 gttgttctat acgactgccg ataacatacg caaaggagcc gctgcgaatg ccgtacagat
    10141 tgctgcagct ttaatgcaga aaatagcgga ggtggcaaga tgagtaatac gctgaaagta
    10201 gcgaaattcg gcggaacatc gatgtctcat gaagagcaat tcaaaaaagt ccgtacaatt
    10261 gttctttcag atcccaatcg ccaagtgatc gttgtttctg ctttaggcaa acgtacgtca
    10321 gatgatgaca aagtaacaga cttactttat ttgatttatg cttaccttca gcatcatgtt
    10381 gattggacac ccctttggaa aaggatttgt gatcgtttca ttcaagtaag agatgttctt
    10441 gccctgcagt tacctattga agatgaacta gcacgcatac agaaacaact ggaagacgaa
    10501 aagataactg aagattattt agtaagtaga ggtgaatatc tgactgctca actgatggct
    10561 gagtatcttg gttaccagtt tattgacgca aaagacctga ttttctttga ctatgatggg
    10621 aaaatcgatc aggaaaaaac agcacaggaa atcacaaaag catttaaaag atatccgaaa
    10681 atggttgttc cagggtttta cggggcgaac cctgcaggaa aagaaaaatt attgggccgt
    10741 gggggaagcg acattaccgg cgcgatactg gcgaagatgt tgcaagcgga aagttatgag
    10801 aattggacgg atgtttcagg aatcatgatg gctgatccgc gaatcatcga tcatcctaaa
    10861 atcatcgagg aaatctcttt tagagaactg agagagatgg cttatatggg ggctaacgtt
    10921 cttcatgaag cagctgtttt cccagttcaa gaggctgata tccccatcca aataaaaaat
    10981 accaatgatc ctttcgctca agggacaaaa atatcgaatc acgaaataga aaaggaaaat
    11041 ggactgacag gaatagctgg acgcaaggat tttctttcca tcactttatt caaacgtcat
    11101 atgtccgatg aaatcgggtt tatctggaaa gcaatgagca tttttgctaa acatgagata
    11161 tcaatcgaac atattccttc agggatcgac aatatcggag tgatcgtctc agcagaagcg
    11221 attgccgata agttatttct catcacgaaa gaattaaaag aaacacttgg cgtggaagaa
    11281 atcgtcgtga tcgaagactt agctttgatc tctgtcgttg ggggtccgca taaagaattg
    11341 attggattat ctgggaaagt gttatcgata ttgaacaaat tagaaattcg cacatcgatc
    11401 ttatcacaag gagcacagga gttgaatctg atcatcggtg taccgaataa tcaatatgaa
    11461 acagtagtga agggtatcta tgaagggatg gtgaatacgg atgctgaaag aatatcgaat
    11521 ggcactccat caaattcctg aaatcgggtt tcatgaattc aagacaaaaa aatatttata
    11581 tgaacaagta aaaaacagcg aggccgttat ccatgaaatc ggcgaaacag gactactcct
    11641 ttattttgat aatcagcaag aagaaacgat tgcttttcga acagatatcg atgcactgcc
    11701 agtcaaagaa gaaacgaatc tctcttttgc ttctaagcat gaaggttata tgcatgcatg
    11761 tggtcatgac ggacatatgg cgatgctttt agggttgact gactttataa ctaaacacat
    11821 tgaggatctt atttataatg tagtactgat ctatcagcca tcagaagaaa tttccggtgg
    11881 cgcacaaagc gttattcagt caggattgtt ggataaatat gaagtacaag caatatttgg
    11941 ctttcatcta tggcctggac tagctaaggg agaagttttc tctcgaccag gacctttgat
    12001 ggcacaaagc agtgaaacag acattatcat taaaggccag tctgcacata ttgcttctag
    12061 tgaaaaagga atcgatagtc tggaggcagc tgtacgattt ttgaagaatg tttatgattt
    12121 tgaccggcaa ttaccggcag agtggaatca tctaatgaaa tttggccaaa tccaaggtgg
    12181 aacgatccga aatgttttag ctggagaagt tattgtatcc ggaagtatcc gttcttacag
    12241 ttcagatatg caaaattatt tgaagagggg attagaccat ttagcagaac agtttcactc
    12301 tacagctact atttcttttc gctataatga cggctatcca gctgtaatca accatagagg
    12361 cttatatgca gcactgttag attcaaacaa actacatgaa ttagatgctc cagttcttca
    12421 agccgaagat tttggcatat atacgcaaca atatccatgt gtatttttct ttttaggtgt
    12481 cggtgatacc cctgctttac atgatccgac gttcgatttt gatatggatg ttttagaaaa
    12541 tggtgtagaa tggtacaaaa cgattctttt tacagaaaat cttcttgtaa aaatcgatag
    12601 cagttcaaaa gaggagcgag taaataatga tgaatttatc caatcgattt agtcctaggc
    12661 tttctagaat agaagtatca aaaatacgac aattcgatca gcaaatatcg tcgataccag
    12721 atgtgatcaa actcaccctg ggagaaccgg attttccaac tcctgaacat gtcaagcaag
    12781 caggaatagc agcgattgaa gaagactttt ctcactatac cggaatgaga ggattagagg
    12841 aactgcggga agcggcttgt atatttcagc aacagcgtta tggattaact tatgatccgc
    12901 aaacggaagt tttgacgaca gttggtgcaa cggaagcgat tgcctctgcg ttgttatctg
    12961 ttttggaaga aggagacaag gttcttattc cagcgcctgc ttattcagga tatcagccat
    13021 tagtggaact agctggtgct gaattgatcc cgatagatac ttctgacaca gggtttgtct
    13081 gtcagccgga acaatttgaa cgagcatttg aacaatatgg atcagctgtc aaagcagtca
    13141 ttttaaacta tcctaacaat cctactggga cgacattgtc tgcaaaacaa ttgaaagcaa
    13201 tcgctgaggt tttaaaaaaa tatccggtat ttgttataag tgatgaagta tatgctgaat
    13261 tgacttatag tggaacgcat atgtccatag ctacttatct tccagaacaa acaatcgtga
    13321 taagcgggtt atccaagtca catgcgatga ctggttggcg agtgggcttt atttttgctc
    13381 aaaaagccct tattgacgaa ctaattaaag tccatcaata tctggtgact gccgctacaa
    13441 cgatgtcgca aaaagctgcg gctgaagcgc taataaatgg ggtgaaagat agcgagaata
    13501 tgaaagagca atatcaaaca cgaagagact atctggtcca gcaattggcg cctttaggat
    13561 ttgaagtgac ccagccaaac ggtgcatttt acttgttttg caaactccca gctgccattc
    13621 aaactgactc ttggcagttt tgtgtagaat tagcggaaca ggcaaaagtt gcgtgtatcc
    13681 caggaattgc atttggccca gaaggagaag gttatatccg gatttcttat gctagcagca
    13741 tggaaaaatt acatgaggcc tgcaaacgga tcaaagactt tttaacagaa cagtgattca
    13801 tagattttat caaaaaaaca atcgttaatt ttgcggtata ttctgcaagg agacgattgt
    13861 tttttaaatt tagttcaaat gaacgcgttt cttttgattc ataactaatg taatgttaag
    13921 tttaaattgt ttagaatgat tttaaaagaa tattcaagga ggtttcatca atggaaaaaa
    13981 tcaaagttgt cattgtaggt gcatctcatg gagggcatca atcgatcttg gaactattga
    14041 cacgatacgg tgaaaatgta gatatcacgc tatttgaagc aggagactat atttccttca
    14101 tgtcatgtgg tatggagctg tatttagaag acaaggtaac agatgtaaat gatgtacgta
    14161 atttcagacc agaaaacttt ccgcaatcca atgttcatat tttaaataat cacgaagtaa
    14221 aaacgatcga tgccgataaa aaaacagtaa cggttactag cgcatcagat aacgaaacaa
    14281 aagaatatcc ttatgacaaa ttgattttga gttctggtgt caaaccaaac aatctgccag
    14341 taccaggagc agatctagaa aatgtctact tgatgcgtgg atacaattgg gcaaccaaaa
    14401 tcaaagaaaa attaaatgat ccggctatta aaaaaatcgc tgtcgtaggt gcgggttaca
    14461 ttgggatcga agcagcagaa gcaagccgaa aagctgggaa agaagtcgta ttgcttgatg
    14521 tgatcgatag acctttaggc acttatttag atgctgagat gacagatatt ttagaacaac
    14581 acctaaatga aaacggtatt gaagttttga caaatgcaaa aatcgaagct tttactggta
    14641 acggaaaagt tgaggcaatc aaaacaagtg aaaaagaaat cccagcagat ctagtcattc
    14701 aagctgccgg tgtgaaggca aatacagaat ggttaaaagg gatcgtcgac ttagatgaac
    14761 gtggctggat ccaaacaaat gaatacttgc aaacgaatct tccagatgtg tatgcagtag
    14821 gagatgctac acttgcttat tctatcccag cacgcaaaaa aatgccgatc gctttagcga
    14881 ctgttgcacg tagagaagca cgttatgtag taaaacatct ttttgaagaa gtaccaaaca
    14941 aaccgttcgg cggagtcgta ggttcctctg cattaagtgt ctttgactat cattttgcag
    15001 caagcggatt gaatagcttt acagcagcaa aagcaggagt agatgtacga gtgtcttatt
    15061 atgaagatac gttacgacca aaatatgtac cagaagaaca aggaaatcca aaagtgtcca
    15121 tccaattatt ctcggatcca atgacccatc aattgctagg aggagcagta ttatctacct
    15181 atgatgttac aggtcaagga aatgtattgg cactagcgat ccaaaacaaa ctgactttag
    15241 aagaactagc agaagctgac ttcttcttcc agccaggctt tgaccgacaa tggagcatgt
    15301 tgaatctagc cgctcaacaa gcgttaggag aagaaccatt tgtagaataa gaaaacgaaa
    15361 tatttgaaaa gaggttgtga aaaagctgcc
//
LOCUS       DACEUZ010000063        15315 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.063, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000063 DACEUZ010000000
VERSION     DACEUZ010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15315
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.063"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(45..1340)
                     /gene="purB"
                     /locus_tag="IXG44_001618"
     CDS             complement(45..1340)
                     /gene="purB"
                     /locus_tag="IXG44_001618"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HAQ1658341.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(1353..2486)
                     /gene="purK"
                     /locus_tag="IXG44_001619"
     CDS             complement(1353..2486)
                     /gene="purK"
                     /locus_tag="IXG44_001619"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HAQ1658342.1"
                     /translation="MINLVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVS
                     DCPAAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTD
                     LLAITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKG
                     QYVLKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHN
                     NILHETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRP
                     HNSGHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENK
                     PDWQFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            complement(2470..2958)
                     /gene="purE"
                     /locus_tag="IXG44_001620"
     CDS             complement(2470..2958)
                     /gene="purE"
                     /locus_tag="IXG44_001620"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HAQ1658343.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            complement(3374..4681)
                     /locus_tag="IXG44_001621"
     CDS             complement(3374..4681)
                     /locus_tag="IXG44_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HAQ1658344.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(4684..5265)
                     /locus_tag="IXG44_001622"
     CDS             complement(4684..5265)
                     /locus_tag="IXG44_001622"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HAQ1658345.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(5381..5478)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(5837..7129)
                     /locus_tag="IXG44_001623"
     CDS             complement(5837..7129)
                     /locus_tag="IXG44_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ1658346.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(7126..7749)
                     /locus_tag="IXG44_001624"
     CDS             complement(7126..7749)
                     /locus_tag="IXG44_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1658347.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(7752..8588)
                     /locus_tag="IXG44_001625"
     CDS             complement(7752..8588)
                     /locus_tag="IXG44_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1658348.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(8605..9492)
                     /locus_tag="IXG44_001626"
     CDS             complement(8605..9492)
                     /locus_tag="IXG44_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1658349.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(9508..10791)
                     /locus_tag="IXG44_001627"
     CDS             complement(9508..10791)
                     /locus_tag="IXG44_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ1658350.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            complement(11474..12157)
                     /locus_tag="IXG44_001628"
     CDS             complement(11474..12157)
                     /locus_tag="IXG44_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAQ1658351.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(12175..12792)
                     /locus_tag="IXG44_001629"
     CDS             complement(12175..12792)
                     /locus_tag="IXG44_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HAQ1658352.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(12802..13626)
                     /locus_tag="IXG44_001630"
     CDS             complement(12802..13626)
                     /locus_tag="IXG44_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HAQ1658353.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(13619..14179)
                     /locus_tag="IXG44_001631"
     CDS             complement(13619..14179)
                     /locus_tag="IXG44_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1658354.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            14353..15309
                     /locus_tag="IXG44_001632"
     CDS             14353..15309
                     /locus_tag="IXG44_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ1658355.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
BASE COUNT         4495 a         3417 c         2622 g         4781 t
ORIGIN      
        1 tttttgctat tctattttaa ttgaattttg caactcactc ttctttattc caaacctact
       61 cgtttgaata tcacgtcaac gtttttcaaa tgatgatgat aatcaaaggc atcgtctatt
      121 tcttctttag ataataccga tgtgatggtt ggatcttctt ctaacagcgg gcggaactgc
      181 acttggttgt cccaagcgta cgcagttttt ggctgaacaa gatcataagc ttcttctctt
      241 gtcattccct tatcgatcaa tttcagcata acgcgctggc tataaatcaa accaaatgtt
      301 gcagacatat tccgcttcat gttttcagga aagaccgtta gatttttaac gatattgcca
      361 aaacgattta acatgtaatt caataaaatc gtggtatcag gcaagatgat tcgttctgct
      421 gaagaatgag aaatatctct ttcatgccat aatgtgacat tttcataggc agtgaccatg
      481 tgtccgcgca ccactctggc tagaccagcc atattttctg aaccgatcgg attccttttg
      541 tgcggcatag cagaagaacc tttttgacct ttcgcaaaaa attcttctac ttcacgtgtt
      601 tcggattttt gcaatccgcg tatctctgtt gcaaaacgtt caatacttgt agcaataagc
      661 gccatcgctg agaaatattc agcatgtaga tcccgaggga gaacttgagt agagatttct
      721 tgtggacgga tccccaattg tttacataca tactcttcaa taaatggcgg gatatttgcg
      781 aacgttccca ctgctcctga gatttttcct gcttctactc cttttgccgc atgttcaaat
      841 cgttcgatgt tccgtttcat ttctgaatac catgtggcaa gtttcaatcc aaaagtagtt
      901 ggttcagcat ggacaccgtg tgtccgaccc atcatcactg tatatttgtg ttcttttgct
      961 ttttcgccaa tgatttccgt aaaacgtttc aagtcttccc gtaaaatatc atttgcttgc
     1021 ttgatgagat agccataagc agtatctacg acatccgtac ttgttagacc ataatggacc
     1081 cattttcttt cttctcccaa tgtttcagaa accgctcgtg taaacgcaac gacatcatgg
     1141 cgtgttaatt tttcgatttc caagatccga tcgatatcaa atgatgcatg ctcacgaatt
     1201 tttttcacat cttcttgcgg gatttcacca agttctgccc aagcttcatc tgctaaaatc
     1261 tcgacttcga gccacgcacg gtatttgttc tcttccgtcc agatattccc catttcaggg
     1321 cgtgtataac gttctaacat gttttttctc ctctaatccc aaatattcgt ttgtttgatt
     1381 tcttccagcg tttgctggat gtcttctgtc aagatagtga tatgtcccat cttgcgacct
     1441 ttttttgctt gtttttttcc atagaaatgg aattgccagt ctggcttatt ctcaatcaag
     1501 cgatagcttt ccagcagttg ttctcccaaa acattgacca ttactgcatt gcttatcaaa
     1561 cgtacttttt ctcctaatgg ccaaccacaa atacctcgaa tatgggcatc gaactgactc
     1621 atagaacaag cttcaataga ataatgtcca gaattgtgag gacgaggagc taattcattc
     1681 acatatagcc cgcctgtttt tgttaaaaac atttctaccc ctaaaacacc tgaaaggctc
     1741 actgcttctg caatcacacg ggcaatacgc tcagcctcct cgatcacatc ctgatcaatc
     1801 gcggctggag caatcgtttc atgaagaata ttgttgtggt ggatgttttc cacgaccgga
     1861 aaagtcgtga aatctccttg accatttcct gccaccatga tcgaaatctc tttttcaaac
     1921 gggatccatg cttctaaaac acaggttcct tctcttaaaa ggtccatggc aggagctaga
     1981 tccgcccgac ttttcaaaac atactgcccc ttgccatcat agccgcctcg agtggttttc
     2041 aaaacacaag gatagccgat cccgtcaatc gcatcttgga tatccgtcgg actgacgatc
     2101 gttgcataag gagcaatcac gatgttattc gtttccaaaa aagatttttc taacaggcgg
     2161 tcttgcgtaa tcgcaagcaa gtctgtccct tgaggaataa atgacatggg taaaatcgca
     2221 tttaacgcct ctacactgac gttttcgaat tcataggtaa tgacatctgt tcttctggca
     2281 agctctttca gcgcaaaggt gtcatcatag acactttcaa tgtgccaatc tgcaacttgt
     2341 gcggctggac aatcgctcac aggatccagc acacctactt taaagcccat ttcttttgca
     2401 ctaatcgcca tcatacgacc taattgaccg ccaccgacga tcccgatcgt tgaaccaggc
     2461 aacaaaggtt taaccaagtt gatcactact ttctataaca gttttagcca acattgaacg
     2521 tctttcagcc aatttagcct ccaattctaa atcatacatg gaaagcatct gtatcgcaag
     2581 tagtccagca ttaattgctc ccgcttttcc gattgctgta gtagctacag gtactcctcc
     2641 aggcatttgg acaatcgaaa gaagcgaatc caaaccattt aatgttcgtg attggaccgg
     2701 tacaccgata accggcaaag ttgtttttga tgcgaccata cccggcagat gcgcggctcc
     2761 ccctgctcct gcaataatga ttttgattcc tttttttcga gcgttttgtg catattcaaa
     2821 catcaaatct ggggttcgat gagcagaaac gacctttttt tcataagaca caccgaattc
     2881 atccaatatt tgacatgctt ctttcattgt ttcccaatct gaagtactcc ccataatcac
     2941 agagattttt gctgtcattg tttttcctcc cagtttttct acattcatat tttaacaatc
     3001 actttttttg atgtcaaaga aatttcgaat gttaacaaag aaaaacattt tattgttcgt
     3061 gtttattaaa gataaaaaaa aggaatgtga caaaagttag gtcacattcc tttttatgga
     3121 taaacggtgt tctcctgcca actcctcggt cacaaggaac aatattcaac aaacatgaga
     3181 agcttttttt tgaaattgtt ccttgtgact cggagatgaa cggcaggctc acgacctctt
     3241 ccttcaacgg tgttcaatca gctgaccctc ggtattagct caaacaaacg gcaaaatacg
     3301 aggaactatt tttccgtttg tttctctaac aatcaggtca aaacgctgat ttcacaacct
     3361 ttcttttgct ctattacttc ttgaaggctt cttccaatat ttcttcatct tttttcaatc
     3421 cattaaagat caaattcaat atgatagccg taatgctact cattacgatg ccatttccag
     3481 tgaacatttg caatgtttgc ggcatttgac tgaataatgt cggcgtaaca ttgaaaccca
     3541 gcccaaatcc aacagaaaca gcaacaatca atagattttt atcattgctg aaatcaactt
     3601 ccaacagcat acggattcct tggactgcca ccatcccgaa catgactaac attcctccac
     3661 caagtaccgg ctcagggatg atttgtgcta acgcaccgat tttagggaat aatcctaaaa
     3721 caatcaagaa gaatgcagag aaatagatgg gttttcttgt tttgattccc gaaagttgaa
     3781 ccaatccgac attttgagaa aagccagtat aagggaatgt attgaaaata ccgcctaaga
     3841 taacggcaag tccttctgca cgatatccct ttttcaattc ttcttctcca acatttttcc
     3901 cagtaatgtc accaagtgca aaatatacac ctgttgattc taccatgctg accatggaaa
     3961 tgatgatcat tagtaaaatc gaccatacgt cgaaagttgg tttaccaaaa taaaagggtt
     4021 gtggaacatg gaacaacggc gcatctccaa tcgcgctcag atcaatcatt cccatgaaag
     4081 cagctaagat gcttcctccg actaaaccaa tcaataccgc gattgaacga atgaaccctt
     4141 ttgccagcat ttgcacgcct acaattagga gaatcgtcac aaatgcaagt agcagatttg
     4201 ttttgtcacc aaaatcagct gctgttgcat tacctccacc cattttctcg attgctacag
     4261 gaatcaaagt caaaccaata acggtgataa ccgttcctgt tactatagga gggaatagtt
     4321 ttttaatttt agaaaagaca ccagaaacta acacaacaaa gatcccagaa gcgataatag
     4381 atccgtagat agctcctact ccttcattgg ttccgatcaa gatcagtgga gctactgctt
     4441 ggatcgcaca tcctaaaacg acaggcaagc caattccgaa aaatcggtta actgtcaatt
     4501 gcaataatgt cgctaatcca cacataaaga tatcaattga aatcaaataa gtcatctgtt
     4561 cttgattaaa gcctagtcct gtaccgatca aaagaggcac cgctactgct cctgcataca
     4621 tcgctagcag atgctgcagc cctaaaaccg cagcttttcc attttgtgtt tctttttcca
     4681 cgattatgcg tcctcctcaa gaaactcaac ttgtccattc gtcaaggaag caatacgcgc
     4741 aagagaaacg actctcaatc ccatttcttc aagtaactga cgtccatctt ggaaagactt
     4801 ttcgatgacg atcccgattc cttctacttg cgctccagct tgctggcata attcgatcaa
     4861 tccttttgcc gcttgtccgt ttgccaagaa atcgtcgata atcaatactt tgtcttctga
     4921 agataaaaat ttacgagaaa tagaaatcgt acttgtcact tgtttcgtaa aagaataaac
     4981 agaagctgtc aataattctt catccatcgt caaacttttt gcttttctag cgaagatcat
     5041 tggaacatct aattctcttg cagcaaataa agctggtgcg atcccagaag cttcaatggt
     5101 cacaactttc gtgattcctt gttctttaaa tacttctgca aaccgttttc ccatttgttc
     5161 catcaataca gggtctactt ggtgagtgac gaagctgtcg acttttaata cgccctcgcc
     5221 taatatacgt ccgtcttttt gaatacgttc aactaattct ttcactttat ttgctccttt
     5281 atcatttatt aataccgaaa taaccgcttt tgtgcaaaaa cctactatgt ttctgttaaa
     5341 gaaaaacacc ctccacttta tcataagaaa agaagggtga aatagccaat cttttactta
     5401 tagtccagca tttacggtac tggctagaaa ctgtcatcca tattatgacg atatataagt
     5461 aggtatgtaa aactaataat agtgtaaatt cattttcttg caactgtttt gcacgtgaag
     5521 gcagtttgcc atatttgaat aaaaaatgtt gtttcaaata ttgaaaacag tctaacacta
     5581 ctttctgaaa ctgacaacgt ggtttactta tttttttgta aaatctaatg atttttaatg
     5641 cattattcct ttttgttcgg gtttaaatag ttatttttat tttctcctat ctaaaaaagc
     5701 agcccacata aaaagaaggc atgacaaaaa gcatgtcata gccccttttt ccgaataaat
     5761 agtgttcaaa agacagccta tcggcaataa ggcaagattt tataaactac gggaagtaat
     5821 ttccgtaaat ctattcttat tcttttaagc tgacaagctc gtcaatcact actgcttttc
     5881 ctgaagcaat agaatgattt gctgcgattc ctgtcaaaat cgacaaaata ccatctacgt
     5941 gagttgctgc ccgattccac ggatcttctt ctttttcttc accaaataga tcatctaata
     6001 aacgcggatc tcctccgcca tgtccgcccg cttcttgttc gacgattacc tcatatggct
     6061 caccgaacat cggatagaca tggatggact gaagaacggc tgctccttca tcctttttat
     6121 ctccacctgc attgatgtaa gacttttcga tcactttcat ttcgatccgt cctttgctgc
     6181 cattgaaaga aacattgaat ccttcccacg gcatatacgc gtttagcgaa tacgtcaaaa
     6241 tcgcaccagt ttcatatttg accgttacgc ctagtgtatc ttcaatagag atatcatcgg
     6301 aaaatacact gcggtcacga atatatccac tgtctttttc tgcttctaaa tacatttctc
     6361 gcaattcctc attggctgac aggtctatag caaatggatc agctttagct agttcgcttc
     6421 catagcatcg actgtaaaat atttctactc cgcgtttctc cgcattatgc atcccataaa
     6481 agtttaatcc accgaatgca aatacctttt ggggacggct gttcaaccaa aaattcacta
     6541 gatcaaaatg atgggtagat ttatgaacaa gaagcccgcc gctattgtgc ttatttcgat
     6601 gccatcttct gaaataatct gccccgtgtt gcgtatttaa cagccattcg aagtggatag
     6661 aaaacacatc accaatcaca tcactttcta tcagttcctt tatcttcgtg tgatacggtg
     6721 cataacgata attaaaagtg actcgcaggc ttcttcccgt ctgtttttgg accgttatta
     6781 tttcttgagc tttttcctca tcgatagtca gaggtttttc gcaaataaca tcatatcctt
     6841 ttttcattgc acgaatgata taatcatgat gcgttcgatc tactgacgta ataatgactt
     6901 cagccggccg tacgtcatcc agtagttgct caaattgtga tggcatacat gtctttactt
     6961 ttttctgtcc atacttgttc acaagtatcc gatttgcata atccatcctt gtttgagata
     7021 catcacaaaa accgactagt tcacaagttt gatgatacgt agaggcaatt gcctcataaa
     7081 aaaaacgcgc tctcccacca agtccgactt gtacatattt tttcttcatt taaagtcatt
     7141 ctccttttct tttaagcaaa atactgtgac aaatggcagc cctggataca gtaatgcagt
     7201 gagtaaaaaa tcatgctgta tacaccagaa cagtccatac aaaaggcata aataccacag
     7261 aaaccccttg acgttttttg acaacagcca gatattcctt ttgatcaaat aaacaagact
     7321 atctgcttta tcttctttgc agatatcaat tagcgtacta aaaaaactat gggatacaaa
     7381 aagcaacaga cacaatagaa aaggaatcag ataccaagct ccaatctttc ccactaataa
     7441 ataccaatca actgcaaaaa taatgacaaa cagctgaaac aacgtaatga tgaaacacat
     7501 ccgtttgatt tttcctttcc tccaacatac ctttttttct gccaagtaat ggacagatag
     7561 ataataagaa ggatacatca tcaaaaaata aatcagatag acaacacgag cagacagcac
     7621 taatcctaaa gtgaacaaac atcctagaac taaagcgaaa agagcattac tgataagact
     7681 ggttaaaaaa atccgacaga catagtagtt gagcttgtaa agaaacatct cttctattgg
     7741 ctttttcata ttcagccctt cagaccgctt gtactgatcc cctggacaag atatttattg
     7801 aagaataaga aaatcagaaa aaccggtagc agcgacaacg tagacatcgc aaacatactc
     7861 gcgtaagtcg tactagatcc agcgtctgca aataatttga tggcaatcga taccgtgtaa
     7921 gatctcggct tgtttaaata aatcaatggt cccatgaagt cgtcccattt ccaataaaac
     7981 tggatgatga tcgtcgtgat gatcgaaggt ttcagcaaag gaaaaataat tttggtaaag
     8041 acactatact tgctgcatcc gtcaataatc gctgattcat ctaactcttt ggggatgctg
     8101 accatgaatt gcatgatctg gtaaatgaaa aatgcttgtc caaaaaaatg tggtaaaatc
     8161 agcggcacat acgtcccaac caaattcagc cgattgtaaa taataaactg cggaatcaaa
     8221 atcacttgtg caggaatcat catcgttact aacatgacga cgaaccagaa tctttttcct
     8281 ttgaaatcga ttctcgataa tgcgtaagca atacaagtcg agctcagaac tgtccctatc
     8341 gtcgaaactg cagagatgac aaacgaattc ctaaagaaag taaaaaatga aattccagca
     8401 aaccccgccc atccttttgc gtaattttcc cattgaaaag tctctggaat caaattcagc
     8461 gaaccactca taatctcttg atccgtttta aaagaacctg cgatcatcca taagaccgga
     8521 taaatcatga ctagacctaa tagcaaaata aaaccgtatt gaaaccattt cgttactgta
     8581 ttattcattt cttcctcctc tcattcacac aacgacccat ttttttgatg atttgaaaat
     8641 cagaaaggtg acagctccca taatcaacag catgatccac gaaatagcac tagcataccc
     8701 catagaatta aaagaaaaag cttggttata cacataaaga gcataaaagt tcgtggcatc
     8761 attcggacca ccttgggtaa tgataaatgc ctgcgtaaaa gtcataaacg ccgcgattgt
     8821 ctgcataacc aagttataca aaatgatcgg cgaaagaaga ggtaacgtga tcttgtaaaa
     8881 cgtctgccat ttgttcgctc catcgatctc tgccgcttca taatacgagc ctgggatctc
     8941 tttgatacct gcagcaaaga tgatcattgc cgaaccgaat tgccaagcag acatcaaaac
     9001 aagagggatc atgaccagtt tttgatcgct aaaccaggaa agcgcaggtg cgcctaatcg
     9061 ttccaggaaa atattcacta acccgttttt agaaaacaac tcccgccaaa ctagagcgat
     9121 agaaatactg ccaccgatca aactaggcac ataatacaaa gaacgaaaga aagtcgttcc
     9181 tttgatattc ctcgttaaaa gataagcaat caatagacca aagatcagtt tcacaggtac
     9241 agatgcaaat acatagagaa aagtgactcg gatggagttc aaaaaacggg catcactcag
     9301 catctttata taattatcca agccgatcca tgagactgga tttcccaaac gataatcggt
     9361 gaacgaataa taaagtgaca agatcatcgg tatcactgtt atcaatagaa atcctaggat
     9421 gaatggagaa gcaaatacgt aaccgaccac attgtcttga tccaagaacc gttttagttt
     9481 ttcttttttc atgccttcct cctgctgcta tcctagaatg tttgacgcaa actcataaag
     9541 actctctgct gcttcttctg ctgttacttc ttcataaatg actttttcaa ctaaaagatt
     9601 gtattggtct tgaatttccg gattcaaagc actatcaaag acattcactt cgccgttgtc
     9661 catatcggaa atcatttcta aataatcaaa tgtcgcttgc aaagttgcat catctgcacg
     9721 agcgagctgt tctcgaatat tcgacatgat tggtacacca cgttctcctt ttaaaatttc
     9781 attggcttct tcactgtttt gaaagaaatc aataaactta gcagcttctt ctttatgggc
     9841 tgagcttttt gccatactca gcatttgcga agaacgtaaa gggataccag attctccatc
     9901 tgcttttctg cgaggtggat taatcaactg aatctccata tcttcaggag cccctgacaa
     9961 tatttctgtc aactggttac ttgatagaaa caccattgcc gcttctctat ccctaaccgc
    10021 ctgaaaagac agatccgtcg tacttgcagc tccaccacct ggttctggat gggccccttt
    10081 gtctaccaga tttttacgca gctggaaaaa atctgttaga tagtctggat tttcaaatcc
    10141 caagctgtta gatagatctt cccgattata aaaattcaat cctttctcaa cttgtggtat
    10201 ttgaataata cagccagcta taaaatcttc gaatcttgaa atagcataga ttcctaattc
    10261 ttcagtgatt tgatagctga tttgttcaaa ttcatcccat gtccaatttg aagctggttc
    10321 agggatattt aattgttcaa atatattttt attgtaagca atcgcaaacg aattcacccc
    10381 gttagacagt ccaactaatt gcccgtcgta agtcgtcgtt gctaaatagg attcatctgc
    10441 attagaaata tcaattatcc cttgctccac atattcatct aatggttcaa tctgattgat
    10501 gtattgagga aattgattgt tcagttggaa aatgtcccag atatcatctg atgctgctag
    10561 tgtacctaat tttgtgtaat atccttcata atcataaaat tcataatcga tcgtcacatg
    10621 gggatttttt tcttcataaa gctcgattac ttttactgtt gcatcgtgtc ggacttgcga
    10681 accccaccaa gccatgttca gcgtgatttt tttgtcatct tcttgcccac aagccgtcaa
    10741 gaagaataac ataacactta aagctaagat gattctcatt cttttcttca ttctctgcac
    10801 ctctacttcc attgttattt ttcaatatac tgtattttta tacagaacat atttattaaa
    10861 cgatcttttt caaggggaat aaaaaccaag acttgcctta tcgttctgcc taggatgaac
    10921 aactcaatac tttcaagaaa aattagatag tgctgattct tctttcatcg ctaaaaacac
    10981 ttcttgcttc aatgatggga ggataattac gcaaagaaaa aaggatatca gacaactgcc
    11041 caatatcctt gacatagcta aataagcatg cttcttcgtg tgcgctcttc cctaaaaaac
    11101 agaaaaataa ctagtaattc tgtatgaaat taggtacaat atacacgtcg atgcacaagc
    11161 attgactatg gcaacttgat tggtcagttc ccgctgactt ttcaagcccc gacaatttag
    11221 ctgctactaa attgtcgggt gcctttttta gtttttattc gtttttcatt ctatagaaag
    11281 cgcttcaata aatcaagaca tttttttgtt agaatgctaa taataacaaa aggttttaac
    11341 tcttcttatg tacattacag gtaaaaaaac aaaaaaattt ctcttttttt tgaattctga
    11401 acaaatagta ctggagtaca aaagacgagg aagagccact atctgctctt cctcgtcttt
    11461 tgattctcac ctgttattca tgattctttt tctcagtttc ccgatatttt tccattttac
    11521 gactaaaaag ctctccgcta cttggtggcg tatatgtgat ggcatttgct cctgcttgaa
    11581 tggtctcagc aatactttct tccgttggac cacctgtcgc aatgatagga agttcaggat
    11641 attttttacg aaaatggcgt actgcttctg cagtttcttt acctgcactg atattgataa
    11701 tcgaaatacc agctgctaac ttttcatcta tttctgtata tttcgacgtt accgtactga
    11761 taactggaat atcaactact cgacaaactt cttttactgt ctctaaaggc gtaggaccgt
    11821 tcaaaacgac ccctaacgaa ccttgcgctt ctgcgaacat gctcatgtag gaagaacgag
    11881 caccttgtgt caaaccacca cctacaccgg agaaaactgg aatgtcagct gcctcaataa
    11941 tgcttttagt aatcgctgga tgaggtgtaa aaggatacac agcaattact gcattcgcat
    12001 ttgtattacg tatgatcgca atatccgtag taaagatgat cgaacggatc aattttccaa
    12061 atatcttgat cccgcttgct tctaaaatga tttccggtac tcttacgata tcttttctca
    12121 attccgtcat gatttctggc gtaaattgtt ctgccatcag ccacttctcc tttgctatct
    12181 ccggtcatac tgattcaatt gatacgtctc gatgacctgg atgatttttt gcgcataacc
    12241 ggggtctgtc gcataacctg catcttgcaa tgcttgtgcg gcttcctgat agttggtggc
    12301 cgtaatcact ttttcataca gcgcaggatt ccaatctact ccttggacga acagttgtgt
    12361 atggtcatcc atggactgtt cccatgaatc atacactttg aagtctcctt ggatagttat
    12421 ccattcttcg ttaacaaatt cctttgtttc caggcttact ttcttctgat ccccataagc
    12481 cttgatcccg aaaagattat tgtatttgct agccaaggta ctttttcccc aatttgattc
    12541 taatatagct tgtcctaaaa tgatactagg aagcactcca taaccatctt gtagttcttg
    12601 ggcatgcgga acaagccgtt caataaattg ctgatgggat tgcgcttcat tttcttgctg
    12661 attatcagta aaaggagaag acagattctt caaggaaaaa acaaaggcta agagtatcac
    12721 tgctacaccg gcaaccactg ctggccaacg gatattcttg cgcttttgct ttttccactt
    12781 ttttttcgcc atgatcaatt cctacttttc atttaattca ttcagatatt cttctagcgt
    12841 caattggtgt ttggtgatat actcagcgtt ttctttaccg acataacgta agtgccaagg
    12901 ttcatatgtg atcttagtta tatcttcgtg tccttttgga taacgaacga taaatccata
    12961 ctgcgcagca ttatctgcga tccatttagc tccaggctga tctccgtaac ttgcatccaa
    13021 tacagtagaa ggataactgt tgtaccagtt ttgatcgacg acatcgactg ccagacctgt
    13081 gtggtgttcg ctgtatcctg gctccgtaat cgtttcttta gtaatctttg tcgcctcctc
    13141 ttctgataca ccttgacgac tcatcacatc ttggacattt tgttcgaaaa cttgttgttg
    13201 ggatgataca gaccggtaag ctgagaccat cactaatgga aatccagctg cttcagcagc
    13261 cttggcaaat gcttcatatt ctggagcaat gcgtttgtcg atcaagtatc cgttacttaa
    13321 tgatgccaat tgagtagact catcaatttc tttttctaat ttgtgatctg gaccaaccaa
    13381 aactagcttc caatcgtctg ttttaacatc tggcaattgt ttggacgtac gatcttttgt
    13441 ttctttgtcc tttaccttgc tgctggttgt ttgactgttc ttttctttac tagcagttgt
    13501 tttggtgctt gttcgttcga ctgcttgatt gccggcttga acatgcgaat catcatcgct
    13561 aaaaataaca tagcccattg ctgcgatcat aatcacaact gctccaaatc ctaaaaattt
    13621 attcacttga aaaatccttt cgttgttctt cacgttcccc attcgcctca tgaacgtaag
    13681 tttcttccag attttcattc acccaatcga gaagttcgat tttttctcct tcgatctgtt
    13741 tttgaacatc tgctggcaga cgaaaattca aaatatcatc atagccccat tcatcatatt
    13801 gaatacttac tttcaagtca aggtatctac cagcctgctg attatttttc aaagggatca
    13861 gctcgacttt tgctgctttg ctgctaagcc atttttgcgc gatgcgtgtt ttgatttgct
    13921 tgtactctgc aacggcaacg tttcgttttt cgggaaaaat aatcgtttga cgcaaaatct
    13981 tttgcgttag catccattca taatcagtga aaagattttt tactttctgc atcgcttcag
    14041 atggcagatt tttctcgcca tctttccatt tttgccattg ttctggactg atcagcaagt
    14101 attcatctaa aaaatttttt tcagtttcaa actgttctaa taatacactc ataataagat
    14161 caatatatac ttgatgcacg tttatcccct ccttttaact attctactta agttttgatc
    14221 aaaaagcacc tcttaaatcg ctgctttcat tttttttaat gtttcaagtc ctctcctctt
    14281 tattcttcgt gatttttgtt ataataatag acagacaaga aactcaatcc aactatgaag
    14341 gagcgttttc agatgaaaaa atatcaatgg ggaatcgttg gtcttggtac catcgcacat
    14401 gagtttgccg aaagcttcaa tcaagagacg agcgaactaa cagcagttgc ttccagaact
    14461 tcagaaaaag cggaaaactt cgctcaccgt tacaatattc caaaagctta cggaagctat
    14521 caagaaatgt tagacgatgc tgaaatcgac atcgtgtaca ttgctgtacc aaatagacag
    14581 catatcgacc atatcttggc agcattggaa gcaggcaaac acgttctatg tgaaaaagcc
    14641 atcacgatga ataaaaaaga actggctgac gctatgcgct tagcagaaga aaagaatttg
    14701 atcttagcag aagcgatgac aatcttcaac atgccacttt atcagcagtt acgctcaata
    14761 atggatacag gaaaactggg ggctttaaaa atgatccaag cacctttcgg cagttacaaa
    14821 gaaccagacc ctaaaaaccg gttcttcaat ccagaattag ctggaggagc tctgttggat
    14881 atcggtacgt atgctgtatc tttcgctcgt ttctttttga gctctcaacc tgaagtagtt
    14941 gcctctacaa tggttccttt tgaaacaggc gtggacgaac aatccgtcac tatcttgcgt
    15001 aacaaagaaa atgaacttgc tgctgtcagc ttgacttttc aggcaaaaat gccaaaagtc
    15061 ggtatcgttg cctttgagaa cggttatgtg acgattgctg attacccgcg tgctgatcgt
    15121 gcggagatct tatttacaga tggtacaaag gaattcatcg aaagcgggaa tacatctgaa
    15181 gcaatgaatt acgaaataaa gaatatggta cacatgattg aaggtgactt gcccaatcgc
    15241 tctctcttct tgacaaaaga tgtcatcgaa atcttggatc aaatgcaagt tctatggaaa
    15301 aatatgtaat tttaa
//
LOCUS       DACEUZ010000064        15008 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.064, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000064 DACEUZ010000000
VERSION     DACEUZ010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15008
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.064"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(273..1154)
                     /gene="galU"
                     /locus_tag="IXG44_001633"
     CDS             complement(273..1154)
                     /gene="galU"
                     /locus_tag="IXG44_001633"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAQ1658356.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
     gene            complement(1197..2216)
                     /locus_tag="IXG44_001634"
     CDS             complement(1197..2216)
                     /locus_tag="IXG44_001634"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAQ1658357.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
     gene            complement(2849..3853)
                     /locus_tag="IXG44_001635"
     CDS             complement(2849..3853)
                     /locus_tag="IXG44_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ1658358.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            complement(3866..4915)
                     /locus_tag="IXG44_001636"
     CDS             complement(3866..4915)
                     /locus_tag="IXG44_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ1658359.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            complement(4932..5753)
                     /locus_tag="IXG44_001637"
     CDS             complement(4932..5753)
                     /locus_tag="IXG44_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ1658360.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            complement(5740..6519)
                     /locus_tag="IXG44_001638"
     CDS             complement(5740..6519)
                     /locus_tag="IXG44_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1658361.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            complement(6536..7006)
                     /locus_tag="IXG44_001639"
     CDS             complement(6536..7006)
                     /locus_tag="IXG44_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1658362.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            complement(7018..7437)
                     /locus_tag="IXG44_001640"
     CDS             complement(7018..7437)
                     /locus_tag="IXG44_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HAQ1658363.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            complement(7518..10286)
                     /locus_tag="IXG44_001641"
     CDS             complement(7518..10286)
                     /locus_tag="IXG44_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1658364.1"
                     /translation="MLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNTVSH
                     YLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQPKDL
                     FSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLHDLL
                     EDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLDEVH
                     RLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRIPIR
                     IELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGELKNI
                     VKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLMEKQ
                     QPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHELLVY
                     WTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELEQQV
                     SQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIAAHG
                     YATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILVDMG
                     SLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPEWRL
                     LYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNSDPL
                     FSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQRII
                     RNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLIERL
                     IRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSEDTE
                     FLSIEEEF"
     gene            10486..10710
                     /locus_tag="IXG44_001642"
     CDS             10486..10710
                     /locus_tag="IXG44_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1658365.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            11489..13204
                     /locus_tag="IXG44_001643"
     CDS             11489..13204
                     /locus_tag="IXG44_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1658366.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            13207..14970
                     /locus_tag="IXG44_001644"
     CDS             13207..14970
                     /locus_tag="IXG44_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1658367.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
BASE COUNT         4362 a         3096 c         2585 g         4965 t
ORIGIN      
        1 aaaaaattcg cctttttatt ctactatgaa tcttctggct tggctttcat ttctcttatg
       61 taatcatttt cttttataca acttaaaaaa aaattaaacc attagcataa tattttctga
      121 atctattcac gaaagtcttt ttctatcttt cgttttccac gactcaactg atctagagtc
      181 aaaaaactaa aacgacagtt gcgcaaatga cgcacaagct gccgctttaa taacaaatag
      241 cctttcattc tcagcaggcc acttatttcc agttaacttt ctgttcttag ctttttactc
      301 aactggataa tatatgcact taatgaagtc ccaatttccg aatgttgaag tccatattca
      361 atatttgtct tcatatatcc taacttatcg cctacgtcgt atctttttcc tttaaactct
      421 ctcgcataga ccgcctctgt ctggttaaga gtctgaagtg catccgttag ctgtatctcg
      481 tttcctgctc ctggttcttg agtttctaat atctcaaata ttttaggagt cagtagataa
      541 cgtccgataa tggctaaatt acttggtgct tttcccattt caggtttttc tacaaaccct
      601 tttactctgc aacgaccctt ttcgctatct gttttaggat caataattcc gtatttagat
      661 gtttcatttt ctggtacacg catgacggca acagcggaag cttgtgtttc ttcataatcc
      721 atcatcaatt gttgagtcaa tgggatatca tctttcatca aatcatcccc cagcatgacg
      781 acaaacggtt catttcctat aaatgctttt gcttgaagca cggcatcccc cagtcctctt
      841 ggatgagatt gacggatgaa gtgaagattg acttgagtcg tttcttcaac taaagcgagt
      901 aattctgttt tttccttttc ccgcaaattg ttttccaatt cgatatttgc atcaaagtga
      961 tcttcaatgg gtcttttttc ttttcccgtt acgatcaaga tgtcttcgat tcccgaatcc
     1021 aatgcttctt ccacaataaa ctggatcgtt ggtttatcca cgattggaag catttctttt
     1081 gccatggctt ttgttgccgg caaaaaacgt gttcctagcc cagcggcagg aatgactgct
     1141 tttttgacct tcacttgctg ttcctcctaa acgacgatta actattgatc atctatttaa
     1201 cgaagagaaa attcattctc catttttccg tcacgtaaca tgatttcttt tgctgctttt
     1261 ttgatatctt ctccattata caaaacatta tagattgttt gagtaattgg catctcaaca
     1321 cctagctgtt gagctagctc tactgctgct tttgtcgttg ccacaccctc aacgaccatt
     1381 cccatgtttt ctagaacttc ttccagctta tgtccctgtc ctaacaagtt accggcacgc
     1441 caattgcgag aatgaacact tgtacaagta acgatcaagt cccccacacc actcaaaccg
     1501 ataaatgtca gaggattggc acccatcgcg acaccaagac ggctgatttc tgccaatcct
     1561 cgagtcatga ttgctgcttt tgcatcatct ccgaatccta atccatggat agcgccggca
     1621 ccaattgcaa tgatattttt cagtgctgca ccagtttcta cgccaatcac atcattattt
     1681 gtataaatcc tgaaataatc attcatgaac agtttttgta cataggctgc tgctttttga
     1741 tcaatacttg cagctgtaat tgttgtgata tcatgtactg ccacttcctc agcatggcta
     1801 ggaccagata atacaacaat tgcctgtctt ttttcttctg gaatttcttc agccaaaact
     1861 tctgagattc gtttatgcgt atcttgttct agtcctttac ttgcatgtat aatgattggc
     1921 ttagtattca ttttaccaat aagttcctgt gcgactgaac gaatcgcttt tgttggaaca
     1981 acaaataaaa tagcatctgc gttatccact gcttctgcca attctttata tccgataatc
     2041 gattctggga tcgtcaaatc aggtaaaaaa tgctgatttg tatgatactt attgatttca
     2101 tcaatctgtt caggtacatt tccccaaata cggacatcat gcccgttttc tgccaatgtt
     2161 tgtgctaaag cagtgcccca agatccagga ccaagaactg cgattttttg tttcatggat
     2221 attcctccaa ataaataatt gataatttcc attatagtat acatttctta aagttgggac
     2281 taatttccat tgtttttaag tgtttgttta cttcttttat cgtttttact tgaaataatc
     2341 aatgatatgt tacaatagtc tacattttat ttttacactc gttatgcttt gcttgttcct
     2401 aagaggaagc agttttgaca atgttactac cagcattact attaggaggt taggaaactt
     2461 ctcctagtga gtattgattt aaaatacata agagacagat tgaacatttc taaatctgtc
     2521 aggtcctgca acggacagat tgtctctgtt gacggttgac ataggaactt ttgttttagt
     2581 caacattctg gatacaaaac gctatgtaaa ctggcgcaac tcgttgtact tggactttta
     2641 ctacggatcc atgcaacaac taaatagtac tattgcataa caaaaaatgg ttggcaccca
     2701 catgtcccat taaacagatt ataagttgaa ctgtagataa tgcaaaaaat gtgttttata
     2761 acgtaattga tgccaatatt ttaaaaaaat aaagggagca gttatcgctg ctctctttat
     2821 tttttatatt ctttttatcc agtctatcct aataatcaaa ctgacgatag tagcggcgta
     2881 tctctaatgg atggcagttc aactgctcca catgagcatc gatgcgttgt gtcactacat
     2941 gcatcaataa gaaagacaaa acacgtccgc gatatttttc actgatacct tctactggat
     3001 agtcttttgt gtctatcaat gtataattgc tgcaaatttt tggcaaaagt ttagccacac
     3061 gttcagataa cactctctgt tcatcttcgc ctaaaaataa tgtgactggg gttgtttctt
     3121 ctacgatctc taacgtcccg tgaagaaact cggcagcatg gatagatttc gatggaagcc
     3181 agctttgttc ttcccagtaa cacatggcat atgaataaac agctccccat tgattccctg
     3241 ctccaataaa ataatgcata ctatcacgtc gatgtttttc tgcaaatgat aagccaaatg
     3301 catctgcttt tttctctgct tccactaacc ctgttggtaa ataacgttcg agctgttcat
     3361 agtattctga ataagcttcg aattctccat tcaaatacat caatcgatct gctgccataa
     3421 aaaatttgat ttgttctgtt ccaggagctg gataagtgac cacaaaatcg catagctgag
     3481 acaacttact gttcgcttta tcgataaatc caatcagtgt cgctcctact ttcttgattt
     3541 gttctactgc tttcactact tcttgagttg taccggtaac agaggacaaa ataacgatag
     3601 aatcttttgt taatcttttg tttccagtag tgtaatattc tgcagcatgc tgtacaaaga
     3661 caggcaagtt gcttttccca ttcatataag tgacagcttg catcgaggaa gcatacgttc
     3721 caccgattcc tagataatag acagcgtcaa atttttccgc ataaagttta tcgatgattt
     3781 cttctacttg cggacgtaaa gctagtgcgc cttttattgc tttgatttgt tcttcttcat
     3841 tgaatttcag catgatttcc acctcttata atttactttt caagactgta tcaaagtctt
     3901 caaaaatctt tttgttcaag tcaatacctt tagctactgc ggttttataa gcgaatagtt
     3961 gaaacggaac gacaagcgct aatacagaca gatattcatc acattctatt gctaatccaa
     4021 gtgttttctc gtttcttgct ttttttgtgg taatggtgag agtctctcca acgtaccgac
     4081 tcatatatct ttgcaacttt tgcgaacgtt ccttatttac actttcccct tcaataaaga
     4141 atagcaaatg cgaagcatct gcctccaggt atgggccgtg catataagct tctaactcaa
     4201 atccttgtga cggaacgcgg attgtttcag tcaatttcgt ctctgcttct ttagctgttc
     4261 cccagtttgg accatatcca atggtgacga aacgctgtgc taaacgaaat aaactttgat
     4321 atccatcaaa aaattcttcc gttttttgaa taatcgctgg taaatggttg attattttgc
     4381 gcaactgttg catgtagtct tgttctattt ttttgctgat tttattttta cttattccaa
     4441 ttcctatccc taagagtagt aactgtaaaa cagtcgcaga atagcctttt gtaacgaagc
     4501 ctactgattc taccccggca cctaaatcaa taatttgtgc agcttctaaa gcaagtggac
     4561 tttgcacgtc atttgtgata gcaacaacag gtaacccttg tttttgtatc atttttactg
     4621 cttcaacagt agaagcactt ttcccgcttt gtgacacagc aatcacagta tcgattgaag
     4681 gagaaagttt tccataattg aaaaaataaa aaggttcttg aatatcgaca gtaagatcac
     4741 cataagattc caatgctggc ttcgctgcta aacaagcgtt ataagatgaa cccgttgcta
     4801 agatcagtaa attcactgtt tttttcatat gttctcttgt cgaaaaatca ttctttttca
     4861 agatcgtact tagtacttcc ttttcttcat agatataatc ttgcatcgta aacattttct
     4921 ctgctccttt tttaaaaaat ccctatccac gaaccaaaaa ttccaaagaa agctagtccg
     4981 atcaagatcg ttaatggttt cacttctttc ttcaatagat aaaacactat gccaaatgct
     5041 ccaagacata agatatttgg aacgatatca tcaaaaatac tttgaacagt cacagcattt
     5101 tctcctgaac cgatttttaa aggaatattg atggtgatca tcgtggcagt cattgctcca
     5161 acgaccatca gtccaataat agacgcgcct aatgtcaaac tttccatcat accgtttgcc
     5221 tggatctttt tcaaaaaacc cgttcctaag cgatatcccc aacctgttgc tagatagcga
     5281 aacagaatgt gcggaatatt gaaaatcaaa atgaacaaga tagggcctaa aatatttcct
     5341 tgcatggcta aagaggtacc cacacctgtg gcaatcaatc gcaaagttcc ccaaaagaaa
     5401 gaatctccta accctgcaag aggtcccatc aaagatgttt tgatactatc gatcgcagtc
     5461 acatcaaatt caggatcatc cttattttct tcctccattg cagccgtgat tcctaaaatc
     5521 agtgtaacga tatgtggggt cgtgttaaaa aactctagat gccgtttcaa agctgctgcc
     5581 atttcttctt tcgactgata taactttttc aaaatcggaa tcattgcata aacataagcc
     5641 atattcatct gacgttcata attccaagaa aactccattt gaaaagaacg ccaaaatacg
     5701 tgatttagct cttttttcgt tattttgctg tttttgatct cagaattctt catcttcttc
     5761 tacctcccct gcagttgttt gaagtggtag ttgacggaca ttcataatat tgactaccac
     5821 aactgctaaa atcgcaccaa aaatagctac tccggtcaca ggaatctcta agtaagaagc
     5881 tatagcaaaa cctaagaaaa aatatggtgc aacttgtttg ttgatcagca aacgagcaag
     5941 catcgcaaaa ccaagagctg gaatcaaccc tgtagctact gacaacccct tttgaacgaa
     6001 gtcaggaatc atatttaata gatttccgat cgtattgctt cccaccatat aagaaaccat
     6061 taccacaaca ccaagcatca aggaaagacc gataccggca agcaagtgca tatgctcgac
     6121 tcctttataa tttccttctt ctgcataatt gtctgcttta tggttcataa ttggaatcaa
     6181 aagccctaag taaatatttt tcaatatcaa tgtcaacgta gcaatcggca aacctagtaa
     6241 aagcgcggtt tctgtaccgg ctccagctgt aatagcaaaa gccgtcccta aaatcccacc
     6301 ggtcacaaca tcaggtggaa tcgaagcacc tactgagaac gatccgataa atgctaactc
     6361 taaagttgcg cccataatga tcccagtttt tacatcacct aacacgattc ctgtaaacag
     6421 ccccgtcaca atcggacgag aaagcaacga tgtccctagt gcatattctg attgtgcgac
     6481 aaatgcaaca attcctaata agactgcttg tacaatcatt gaggttcttc ctttcttaca
     6541 aaacttctct tacatccact tttttatctg ctggtacttg acggatttct acttctattt
     6601 gtttgtcaat taatcgtttc aagcgttcct cctcttctgg taaaatattt accgcttttc
     6661 cgatgctgcg agtaccaggt tttgccttga tccctcctaa gttgatggtt ttgatttctg
     6721 gatacgcttc tgccagctta tatgcatcct cgatcgattc cactacgatg aataacttat
     6781 atttatctgt caccccgctt ttcaacgcgg caatcgagtc atcaattgat ttgatcacta
     6841 gtttgactcc ttgtggcttg gctaatttga tcgttgtctt tcgtatctca ttcgtaggta
     6901 catcatcatt agcaatcaaa atacagtctg cgcctattgt ttgtgtccat gaaaaagcta
     6961 cttgtccgtg taataaccga tggtcgactc ttgtcaatac aatcattttt cttcctccta
     7021 aaattcgtct tcttctattt tctgattgag ccgctcgtta caaaactgga tcattttttt
     7081 tgaatcagtt agcacctgct ggatcaaaac agttgtctct acttcagcgt ccaaccgaga
     7141 tactagctca atcaatagtg gcaaattcaa tccggcaacg agatagacat gatcccgatt
     7201 gatatattgt aagaattcgt tgttgacact tccgccaaaa agatccgtca gaacaatgat
     7261 ttctttatct ttgtacctca taaaaagctg ccttactgtt tccgctaaat caaaattctc
     7321 atcagtatag caatcgaata cagtaataga atgatttttc ccatagatca gctctaatga
     7381 atctaatatc cctgaagcaa attttccatg ggaagctaag ataatttctc tatccacttt
     7441 ttcatctcct cttttcactt acacatacta aaaaagcaag ttgcatgcca aaattagtgg
     7501 caacaacttg cttatgttca aaattcttct tctatcgaga gaaactctgt atcttcactc
     7561 aaaatatcat agatataggc aatctcagaa ttggggatca tgacactata atgctcttca
     7621 atgacactaa acgcagattt aatagcagat aacccttctt tttgacactg cttccatttc
     7681 tctaatcctt gataggtttc gattggcatt tgtcggatca atcgttcaat gagacagctc
     7741 acatggacat acaacgctaa ttttttggga ttgctgatgc ttcgattcaa ctgcacctct
     7801 aattctctca taaaatgatc gatttctccc atcaccttct ctgtatctag aatagtcacg
     7861 gaatccagta ctttttccaa tgaaaaatta cgaatgatgc gctgattgaa tatttccttc
     7921 tctgaagcat tcatagtagt gtccaaccat tcagctagta cagacatctt ttcccccgag
     7981 atgaggtctt ctaatgaaag atagtcgata ttggtgatat caggatctgt ggtgccaatc
     8041 attcctacga cttcgtatat ggaaaataat gggtcactgt ttttcttatc ttttaattgc
     8101 tgatattcat atgggatgat tttcaactga caagccgtag gtaagctttt ttctagcagt
     8161 tcacttaagt ggaccgccgt accgattcca gtgaagcaag tagtaatgag tgctttgggt
     8221 ttgttttcct ctggatagag cagtcgccat tctggctgga tctgtcgaac agtctcttct
     8281 gctatctctt ctaatgatac atttttttgc aggcattccc ctactgtgat tgctaaaggt
     8341 gtcgtcacat tattcaaaat caaaagcggt acggataact gttttttaaa aaactgatgg
     8401 atttctttca gagatcccat gtctactaat atgatcaatc catttgaaac atcgctgttt
     8461 tcgcaataat caaggatttc tgaagcaatc tgctgtggtg tgacatctag tggcatatca
     8521 aatgattcaa atagatcctc ttgtaataaa cgatttacta catttgccat actgctagca
     8581 gttgcataac cgtgagcagc aatcatcgct tttggcagtc gtttttcttt tgaccatttc
     8641 gcttttttta gatagatcgt cagtaaaatc cgatccatac ttgtgtcttc aatatctaat
     8701 ttgatcttac ttaattccag aattcgttgg acatagtgat aactagatgg ataagactga
     8761 gaaacttgtt gttctaattc tttgattagt tggataatat catgttcttc cggtaaccat
     8821 ttgatagtgc ttcgctgata gagatagtaa ctcaaggcat agacactgtt tccatcgaat
     8881 ttgatttgat aggcagtttc catttgtcga aaggtctctc ggatagattg tgtccaataa
     8941 accagcagct cgtgtttttc ttgtctactg gtctcaaaca gcaaaaaatc aaataattga
     9001 atcacttctt gctttaattt tccttcgcaa gaatatagca gacagttatt tttcttgaaa
     9061 tcaattagga tttgctcaaa acttttaacg attcgctgct gttcgggctg ttgcttctcc
     9121 attaattgct ctaaggtctg gtcttgcgta atatagattc cttgctgtgc tgtattttta
     9181 gtaataggct gcgcaagtag ttctttttgc aaatgatgga tcgtgacatg gatttcaggc
     9241 tgttcacgct gttcagcata tgcttttgct gctgaaactt tgacgatatt tttcagctct
     9301 cctatgtttc cggataattt ctgacttgtt aaaagtgaca aagctggtcc actgactgac
     9361 aaagggctcc ccatttttct ttgttcttcg agaaaaaaag agtaaatcag ttctagccgt
     9421 tcattacgac tacgctgatt aagtgcaggt agttcgattc ggataggtat tcttcgaatg
     9481 aaagttgtta agaaatgact agtaatctct tcagtcgtag caaaaaagag acgtgttttg
     9541 atccggcgtg acaaattagg ttctcccata cgataaatca caccttgatc aagataggtg
     9601 aacagttttt cctgaccttc tggtgttaag cgatgtacct cgtctaaaaa caagatccca
     9661 ccatctgctg cttcaaaagc ccctttttta ttttcttctg caccagtgta cgctccctta
     9721 acatgtccaa acaaattgct agtcaaaagt tcaggattat ctgcatattg tgcacaatta
     9781 aaagtgatga atggtgcaga atcttctagc agatcatgaa gcagacaata ctgatagacc
     9841 aaattaacta aataactttt acctgtgcca ctttcgcctg taatcaaaag tggtaatccg
     9901 ccatctggat agtaaagagc tgttttgatt tgttcgatac tttctttcaa actggcgtcg
     9961 tatccgatca taaaagagaa taagtctttt ggttgcgcga aaaaaggctg ttcctctttg
    10021 agatcatcga tagaaaaata aaaattcgtg cgtaacttaa aaaattgttt ttcaaaagct
    10081 tctttgtgga aataaatcac tggtcgagaa ttgattttga ctagtagttg ctcatcattt
    10141 aactggttca aataatgact gactgtgttt ctttttaccc caaaatgatc agctaacctt
    10201 tgtgctgtaa agacctcgtt catatgttct aaatcgaaaa aggctgtctg gttctctaag
    10261 taacataaaa tttccttctt caacaactta ttcacccctc ctccatcata gaaaacggat
    10321 tcattttgtc aataaaatct ttaataaaga attgaaaaat atatcttatt atagacagca
    10381 gcctacttat cccttataat ttttattcta aaaaacgtac agttccttcc agtttttata
    10441 ctggaatttc aacatatgaa agaataagga ggctatgact attcaatgaa atcggataaa
    10501 tataaaaaca ggaaacatac ttcctggaaa acttttcgag aaatcatcgg atcttatctc
    10561 gctgtagttg tatttttgcc tttgctttat atggctatga cagaacaaac tcgttttatt
    10621 ggcgtagata cgcaatatat agccgaaaca ggatttttat tcatctttat cttgattttt
    10681 gcgttctata aatccttcca tacaaaataa gattcacaga tttcaacttt cctgtctgtt
    10741 tttagctatt acaaatcctt atatgtggta taacaacaat ttattggcat aatgaggaaa
    10801 aacgagttat gatgctttca gagagtaaag aggtactgtt gtcaccatcc tttttattga
    10861 ttacgtacac cactcttagt tttttcccat tgattacgaa aacaaggtag aaacattgat
    10921 gttcctacct tgttttcttt ttttattttc agatcaaaat ctcaagtagc ttttgacttg
    10981 atgtcgggag gttgtcttcg ttccatcaaa cctgatagaa tctgcgccaa accatagttt
    11041 tctccactat tatttattcc tctaagatag tacccacaat aaacgaataa atatttttct
    11101 cattttcata ctccttcttt gaaaatcaat tagatttctc tcaacccact tttttacaaa
    11161 aattacgtag caaaaaagat tgcaagaaca aattaaaaaa gaagtaaata gagtttcatc
    11221 tataaacatt tacttgatat attttttatg cagaattcat ttatagaaaa attaagtaaa
    11281 atagacgttc ctatgaatga agagactggc atgttttctc attttctttt ttttaaaaaa
    11341 aatagtctat tcaatttcta aagcttcttc ataataaaac tttcatttat gaaaaggctt
    11401 gttctctttt cattctaaat ttcctaagct acaatgtggt agtacttgtt ttttcaattg
    11461 tggacaaata aataatagga ggaatcatat gattcagtta ttacgttatg caaaagacta
    11521 tcgtaagcaa atcattttgg gcccgttctt caaatttctt gaagctgtct tcgaacttgt
    11581 cctgccttta atgatggctt ccttgatcga taatggacta aaaatgaatg accgaggaaa
    11641 aatcattgaa atgggcttat ggatggtcgc tatgtctgtg attggtctga tttgtgctat
    11701 catttgccaa tactatgctt cagtagcttc acaaggattt ggtacagagt tacgtaatca
    11761 gctaatcaaa aaaatcaata ctttctctca caacgagttg aatcattttg gaacagatac
    11821 cttgatcacc cgaatgacaa acgacatcaa tcagcttcag ctagctttag caatggtcat
    11881 tcgtttattg atcagagcac cgtttttaag tatcgggtca gtggtcatgg cttttgtgat
    11941 cgactgggaa gtaggtctgt ttttcttagc attattgcct attttctcaa tcattttatt
    12001 ttttatcatc aaaaaaaccg ttcctctcta ccaaaaagtg caagaaaaac tagatcagct
    12061 caatgaacac gttagtcaga atcttagcgg tgttcgtgtc atccgcgcat ttgcgcagac
    12121 aaaaaaagaa acagagaagt tcgataaaag cacaaatgat ctagcgaaca attatttacg
    12181 tgtttccaat atctctgccc tattatcacc tgctacaacc ttgatcatga atgtcgggat
    12241 catctgtttg ctgacagttg gcgggatcaa agtaaatatt ggtagcttgc aacaaggaca
    12301 agttcttgct ttgatcaatt atatgaatca aatgcttttg gcactgatcg ttgtttcaaa
    12361 cctcgtcgtg atcttcacac gtgccgaagc ttcaggaaat cgggtgaaag aggtgctaga
    12421 cacagaaaat tcgattcttg aagctgaggt cagtactact cccgattttt cttctgaagc
    12481 aattatcgct tttgaccatg ttgacttccg ttatactcct gattctggtc tttctctcca
    12541 aaatattaca tttcagctga agcgaaatac agtccttgga atcattggtc caaccggaag
    12601 cgggaaaacg actttgacac aattgattcc gcgtttctat gatgtcagtt ccggtacagt
    12661 tataatggac ggaatggatg ttcgttcttg gcagctggat catctccgtc agcaaattgc
    12721 tcttgttccc caaacttctg tactatttac cgggactatt cgtgaaaacc ttcaatgggg
    12781 taagaaagac gccacagaaa aagaatgttg ggaagctctg aaaatcgctc aggcagaaga
    12841 atttgtccgt cagcttccaa atggtttaga tacaaaagtc atggaaaatg ggaaaaattt
    12901 ctctggcgga caaaaacagc gactgacgat tgcaagagct ttgattgcca agccagcatt
    12961 actgatttta gatgactctt taagtgcgtt ggattatcaa acagacttgg atttgcgtaa
    13021 ggcgctacag acttatcttg gcagtacagt gattattatc tcccaacgtg tccgctctat
    13081 tcaagaagca catcatatct tagtgatgga tcaaggtaag attgctgctc agggtaccca
    13141 tgaagaatta cttgctcaat cagcagaata tcaggaaatc gtcgcatcac aggaggaaga
    13201 atagttatga aagtaaaaaa attatccttg cagacgctcc gccgattcat tccttacctt
    13261 actgcctatg caaaagacat ttggctagct gtgatactag gaatcatcaa tgggattgca
    13321 acagttgtga tgacgttaca aattggtcaa agtatcgatc aaatgattgg agaaggacag
    13381 gtccgcttca atcgactgtt ccatttactt attctattcg gtggtatcgt tttaatcaac
    13441 gtagtcagtc aatggctcat ccaagtgtta agcaatcggt tagcttactt atccgttgca
    13501 aaattaagaa aagatgcatt tgctcatctg aatcgattgc cacttcgtta ttatgatcaa
    13561 aactcccacg gaaatatcgt cagtcgattt acaaatgata tggacaatat ttccattgca
    13621 atttctgcgg ccttcaatca attattttct ggagtggcaa tcatcattct ttcgtttatt
    13681 tttatgatgc gtctaagtcc acttctgaca ttggtcgttc tttgttctac accaattatt
    13741 ttccttgtga gttggatcgt tgctcgtgct tcacaaacaa atttcgatag acagcaaagg
    13801 attattgggg atatctcggg ctttgtgacc gaacgaatcg gtaaccagaa aatcgtgaaa
    13861 gcttttcagc aagaagaagc tagtcaacag caatttgaac tgctaaatgc tgatctatat
    13921 gaaaaaggac aacgggcaca attttcttct tccttgacaa acccgtcttc tcgttttgta
    13981 gatcaccttg cttatttatc gattggttta gttggcggcc tattggcatt gaagagtggt
    14041 tcgtcaatca ctgttggtgt gatttccagc tttacgatct actcaagtca gttttctaaa
    14101 ccatttatcg aactttctgg aatcacgaca caactgcaaa ccgctttttc tggtctagaa
    14161 cgagcatttg atttgatcga tcagccagaa gaaaagcctg atttgccaga tgcctatgcc
    14221 ttggaaccca aaaatatcaa aggaaaagtc tcttttgatc atgtttcttt ttcttatgac
    14281 cccaagcagc gtttgatcga agatttcaat tttactgctt atccaggtca aaccattgct
    14341 atagtaggaa aaacaggggc cggaaaatcc acacttgtta atttgctgat gcgtttttat
    14401 gacacaacaa aaggaacaat tacagtcgat gaccatgaca tccgctctat ccaacgtgat
    14461 acattacgta aaagttttgg aatggtgtta caagatactt ggctatttga cagtacattg
    14521 cgagaaaatc ttcgttttgg gaatccggaa gcaagtgacg cagaaatcaa tgatgcccta
    14581 aaagcctctt atatgtatga atttgtagaa cgtttgccgg aaaaattaga tactaagatt
    14641 ggcggtcaag ggttaaagat atcagatgga caacgccaac tgttgacgat tgctcgaacg
    14701 atgatcagta atccccctat gttgatctta gatgaagcta ccagctcagt cgatacattg
    14761 acagagaaaa aaatccaaca agccttctta gcaatgatgg aaggaaaaac cagttttgtg
    14821 attgcccatc gcctgtccac aatcaaaaat gcggaccaga tattagtgat ggatcaggga
    14881 gctattgtcg aaatgggcac ccatgatgag ctgcttcaaa aagatggtta ttaccgacgg
    14941 ctctatcaag cacaattcac agcacaataa aaacaagcag tcttctggga tgggaagact
    15001 gcttgttt
//
LOCUS       DACEUZ010000065        14929 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.065, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000065 DACEUZ010000000
VERSION     DACEUZ010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14929
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.065"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            129..1964
                     /gene="tdc"
                     /locus_tag="IXG44_001645"
     CDS             129..1964
                     /gene="tdc"
                     /locus_tag="IXG44_001645"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAQ1658368.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEQVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     EQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKEIAKAMEKKLNEIIQ"
     gene            2269..3747
                     /locus_tag="IXG44_001646"
     CDS             2269..3747
                     /locus_tag="IXG44_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1658369.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLKNPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            3713..5044
                     /locus_tag="IXG44_001647"
     CDS             3713..5044
                     /locus_tag="IXG44_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1658370.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRVMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKANFGQLNELVPFGFAGMIASFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKNAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQSGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFVGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            5158..6468
                     /locus_tag="IXG44_001648"
     CDS             5158..6468
                     /locus_tag="IXG44_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HAQ1658371.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIEARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADVRLIEVHDLLVE
                     ESILTGESEASEKNHSVLAAEESIGDQKNMAFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLILFLVVFTTFRYGMDWHILLSAMIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEETRVLFIMKNCQELKTKEEQKTENLSGNPTELALIRHTETADLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGGVIYVKGAPEVLLAKSTLSSSE"
     gene            6607..7779
                     /locus_tag="IXG44_001649"
     CDS             6607..7779
                     /locus_tag="IXG44_001649"
                     /inference="COORDINATES: protein motif:HMM:NF013991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HAQ1658372.1"
                     /translation="MIDPPKESAVKAVKECQQAGISVKMITGDHKDTAKAIAEQIGLK
                     HTAKVLEGIDLDLMSEEELIRQVPKVDVFARTTPEHKLRIVKALQKNGEIVGMTGDGV
                     NDAPALKRADIGIAMGIKGSEVSKQAADMVLGDDNFHTIAKAVKEGRRILDKSAKTLT
                     PFFEEMAQIAWHYAIDQELRERIAEIGRKAEKAMLIATNGINTHKGAIWAMGLLISVI
                     AQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQKYGVNGAYEEAILGY
                     PHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSDQKTLVEMQKLAHAAS
                     QSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELWTADTYICPMLLND"
     gene            7925..8653
                     /locus_tag="IXG44_001650"
     CDS             7925..8653
                     /locus_tag="IXG44_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HAQ1658373.1"
                     /translation="MGLCLFALPTQGFAVAGGHGGGTSSGRSSGRGSFGGRSYGGSYF
                     GSRSYGRDSYGYYGPSTGPSSYEQTGFLALAVLSVGGVSAFSIVKNKRQKKRLKSEIL
                     SRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFAEHQKVLQKNKAD
                     GVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLISGSKTQRQYFSQ
                     IWYFDFSEKEKRWQADFIQPIMLD"
     gene            9142..11235
                     /locus_tag="IXG44_001651"
     CDS             9142..11235
                     /locus_tag="IXG44_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ1658374.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLEEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            11260..11562
                     /locus_tag="IXG44_001652"
     CDS             11260..11562
                     /locus_tag="IXG44_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1658375.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            11589..12878
                     /locus_tag="IXG44_001653"
     CDS             11589..12878
                     /locus_tag="IXG44_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1658376.1"
                     /translation="MSVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
     gene            12897..13304
                     /locus_tag="IXG44_001654"
     CDS             12897..13304
                     /locus_tag="IXG44_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAQ1658377.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            13327..14196
                     /locus_tag="IXG44_001655"
     CDS             13327..14196
                     /locus_tag="IXG44_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HAQ1658378.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            14198..14818
                     /locus_tag="IXG44_001656"
     CDS             14198..14818
                     /locus_tag="IXG44_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ1658379.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
BASE COUNT         4836 a         2539 c         3243 g         4311 t
ORIGIN      
        1 gtaaaaatct acgtggattt gatgttataa aaaaaaattt ctcatttttg gcgctatagt
       61 gaagaggtgt tacagataaa cacatgtatc tgcgagacat tgaccaacaa aatatggagg
      121 gaagaaaaat gaaagatatg gatatcaagg ccgtctttat aggcgacaaa gctgaaaatg
      181 gaccagttta taaaatgttg ttgaacaaga tggtagatga acatcttgga tggagagaaa
      241 attatattcc ttcagatatg cctgcaatta gcgaaggaga taagctcact ccagattatc
      301 tagccacccg cgaccatatg atagaggtct tggatgaagt cagtcagcga ttacgcgcag
      361 gatcgatccc atggcattca gcaggccgtt actggggaca aatgaatgct gaaacattga
      421 tgcctgcttt attagcttac aactatgcga tgctgtggaa cccaaataat gttgcattgg
      481 aatcttctat ggcaacttcg caaatggaag cagaagtagg acaggatttt gcttcattat
      541 tcaatatgac agacggatgg ggacacattg cagcagatgg ttctattgct aatcttgaag
      601 gattatggta cgcccgctgc atcaaatcga ttcctttagc tgtcaaggaa gtgctgcctg
      661 aacaagtgaa aaaaatgtct gagtgggaac ttttgaattt atctgttgaa gaaattttgg
      721 aaatgaccga aagctttacg gacgaagaaa tggatgaggt caaagcagct tcttcccgta
      781 gcggaaaaaa tatccaaaga ttagggaaat ggctggtgcc tcaaacaaaa cactattctt
      841 ggatgaaggc gttagatatt tgcggagtag gcttagatca gatggttgct atcccagttc
      901 aagaggatta tcgaatggat attaatgctt tggaaaaaac aattcgtgaa ttagctgaac
      961 aaaaaatccc aattctaggt gtggttgctg tcgtaggaac gacagaagaa ggtcaagtag
     1021 acagtgtaga taaaatcgtc caattaagag aaaggttaaa agatgaaggg atctatttct
     1081 atttacatgt tgatgccgca tatggcggct atgcgcgttc actgtttcta aacgaagcag
     1141 gagaatttgt gccgtatgct tccttagctg aattctttga agaacatcat gttttccacc
     1201 actgcgtaac gattgacaaa gaggtatatg aagggttcag agctatttca gaagcggatt
     1261 ctgtcacgat agatcctcat aagatgggct atgtccctta tgctgctgga ggaatcgtaa
     1321 tcaaacataa aaatatgaga aatatcattt cttatttcgc gccatatgtg tttgaaaaat
     1381 cagtaaaggc tccagatatg ttaggagctt atattttgga aggatcaaaa gctggagcaa
     1441 ccgccgccgc agtctggaca gcacatcgtg tattgccatt gaatgtgacc ggatatggtc
     1501 aattgattgg ggcttcaatc gaggcagctc aaagattcag agaatttctc gatcatctga
     1561 cttttactgt taaaggaaaa acaatcgaag tctatccatt gaatcatcct gattttaata
     1621 tggtcaattg ggtattcaaa gaacaaggct gtacagattt gaacgctatc aatgaattaa
     1681 atgaaaaaat gttcgatcgg tcttcttata tggatggcga tgtttatggt gaacggttta
     1741 ttacttctca tactacattt acacaagaag attacggtga ttctccgatt cgttttgttg
     1801 aaagaatggg tctaacgaaa gaagaatgga aaaaagaaca gaaaatcact ctgctacgtg
     1861 cggctatcat gactccttat ttgaatgatg atcgaatttt caatttttat acaaaagaaa
     1921 tcgccaaagc aatggaaaag aaactaaacg aaatcatcca atagaactga tttttgtgtg
     1981 aaagaataac aagagacata tggagaacag acaattattt gcttcatcaa aacaattgta
     2041 tttccccctg tttttcatat attcctcgcc aagatattct tttttataaa acgttctata
     2101 agttggatcg ttcgtttcat gtatcaaacc cagttcacta agatcgttca atggatgttt
     2161 cggtgatact gggttttgta ctgaatcgat gaaaccttgt catttctgtt cttgaacaaa
     2221 aataaaagaa aacaaaaaca tatgtcgcta ttgaaagaag gaataaagat gaaagatact
     2281 ggttcatcaa agcaaatcac gaccatgacc tttatcggaa tgacctgcgc ccttgtagca
     2341 agtgtacgaa atattccaga tgtagcagca acgagctgga cgatgatttt ttacatgctg
     2401 gttgcagtat tattgtacgc ttttccaatc tcattgatct ctggtgaatt tgccggaatg
     2461 tttccccaaa aaggtggtcc agaactttgg gtatcgaatg ctctaggaaa aaaatggggg
     2521 ttcgttgtct catggctgtt atgggtccaa atgtttcctg ggatggtgat ggtcgcttca
     2581 gcacttgctc cgttgtttgg taatattatc gataatgtgc cgcttggttt gaacagcaaa
     2641 tttacattag ttgtcatttt agtcgtttat tggatcatca cttttttgaa tttgaaattt
     2701 gatatggcaa aaatcggcgg aaaagtcggt gtttggttag gattgtacgt gcctttgacg
     2761 atgatgctct tattagggtt tgctgcgtgg atcaaagttg ggatcgtacc aaccggaaca
     2821 ttaggcagtt tttcatggga caaattaata ccagatacta cgactgcatc ctcatttgtt
     2881 tattttgcgc cgatcatgtt tatttttact ggtatcgaaa tgtcctctgt gtatatcact
     2941 cggttgaaaa atccagttaa aacgtatatt cgcggagtat ttgccgcgtt aatttttata
     3001 tttttcgtca atattttgaa tgctttagtt gtagcaaatg tcgtaccaaa aggacaaatg
     3061 gaattgaata atatcgcgca atctatttcg atttattgcc aaattcttgg tttgccacat
     3121 cttattgtga atctgtttag tttacttgtc ttcattggtg tagcagtcca attatctgct
     3181 tgggcctcag gaccagcaaa gacagtgaca gaaagtgcta gaaaaggtgc gtatccacca
     3241 aaattcaatt tttggaagac gaatcaattt gacgtatcca aatccgttat tttgactcag
     3301 tctgtcatta tctcaatctt tgcacttttt tatttgctga ttcctggcgt caatcaagca
     3361 tttttgatgc tcgtaaattc aactactgtt atctactgta tcgtctatgt catcatgggg
     3421 atcgcagttt tgagattgcg ttatacacat gcaaagttga atcgaccatt tagaattgga
     3481 aaaaaagaga aaaagagcaa tcttggagtt tggactgtcg ttattgtttt atttgcggca
     3541 atcggatttt ctgtcggact gacgatgaaa gctgggacat ggatcaatct aattgcagtc
     3601 acagccatca gtgtcatttt gtttgtagta ccactctgta ttgaaaaaat aaagaaacct
     3661 tcttgggaac aggaagtcgc catgctgctt gaaaaagaaa aaggtgaact tgatggaaaa
     3721 accggcaatg aaacggactc taactaatcg aaatatccag ttaatcgcat taggtggcgc
     3781 aattggaaca ggattatttt taggttcagc aaaggcaatt gcatcagcag ggccagctgt
     3841 tattctcgtt tatttgatat gcggtttggc tatttatggt ttgatgcggg tcatgggtga
     3901 tttattttta gcaaatagta catttaactc tatcagtgat ttcgttgact attatttagg
     3961 caaaaagtgg gcattttttg tcaattggac ttattggctt tgctggcttg gagctggact
     4021 tgcggaatta acagcaatag gattgtatat ccatttctgg tttgtacatg taccagcttt
     4081 gataagcgga ggtagcgcac taatgatctt aatggcagtc aaccttgttg cagtcaagtg
     4141 gtttggtgaa ttggaatctt ttttctcaat catcaagatc ttggggattt tcctttttat
     4201 cttattgggt ataggattga gtgtctcaga ttttcatacc cagtctattg gaatcaaagc
     4261 aaattttgga cagctcaatg aattagtccc gtttggcttt gctggaatga ttgcttcatt
     4321 tcctatggtt ttattcagtt ttgctggaat tgaaatgatc ggattaacag ttggtgagac
     4381 gagtgaccca caaaagaatt tgaaaaatgc aatcaactgt ctgccttggc gtatcctctt
     4441 tttctatata ggaacgattt tgtcgatttt atttgttgtt ccatggcatt gtttagattt
     4501 aaaagaaagt cctttagtga ctatgctgca agtgatccat tgccaaagcg gagcagcaat
     4561 tgtcaatcta atgattttgg ttgcttcttt gtcctcagca aatagtgcag tctacagtac
     4621 tagcaggatt ttatatcaaa cagcaaaaga gaaaagtttg ccccattggt tttgtgcgct
     4681 ttctaagaaa ggcgtacctt tacacggtat tttaattaca gggatgttat tttttgtcgg
     4741 tctttgtcta catttttttg tagctgatag tcgattgttt tttcagcttt tggcaggcat
     4801 gacaacgagt tgttttctat ttgtgtggtc ttcgatcttg tgtgcacatg ttcaatttat
     4861 taaagaaaag aaacggaaca ctacacaaag atataaagat cttgctttgt tggtgttttt
     4921 tacttcaatt gctttcagct tgttattcga aagcacgaca cgctttattc cttttttatt
     4981 ggttatctgg tttgtattct taaattttgt atataaaaga ataagcaata gaaatatcag
     5041 ttaggcccag atacattctg caaaacgaat tatttcagac ccccctgaaa taatcgatac
     5101 gcctgaaaaa acttccctgc gtataataac agtatcaatt aaacaggagg ggataggatg
     5161 aactggtaca gattgacggt agaagatgta ttgaaaaact atcaaacatc aacagaaggc
     5221 ttgtcgcaag aagaacggca gaaacgcctg actgatcaag gatataatga gatagaagca
     5281 agacagcaga caaaacgatg gaaaaaaata gcaaaacact ttactgatct gctgatgatc
     5341 gtgttgatca ttgcctcttt tttgaaattt gcttcgagcg aatacatcga aggaagtatt
     5401 attttattcg tagtgatcgt caacggactc gttagttatt ggcaagaaag aaaagcagaa
     5461 gaatcgttga atggtttgaa gcagctgatg ggacaagaag cgatagtact atcgaacggg
     5521 atgcaagaaa aaatccctgc gaaagaacta gtaaaaggag atgtcattgt cttacgtgcc
     5581 ggagacgtcg ttccagcaga tgttcggttg atcgaagtac atgatctact agtagaagaa
     5641 tcgattttga cgggagaatc agaagcatct gaaaaaaatc attccgtttt ggcagcagaa
     5701 gaatcaatcg gtgaccaaaa aaatatggct ttttcaggaa cgcttgtaca atctggttct
     5761 gcagtcggtg tcgttgtaga aacaggaaac acgacagaaa tcggtaaaat caatcaagca
     5821 ctgcaatcgg tcgaacaaca aacgacgcct ctgatcaaga agatcaaccg attgaacaaa
     5881 caaatttttc aaggcattct ttgtttgatc ttgttcttag ttgtttttac aacattccgc
     5941 tatggcatgg attggcatat ccttctttca gctatgatcg ctttggtggt gtcaatggta
     6001 cccgaaggat tgccagctgt gttgaccatg atcctatctg tcggagtaca tgaaatggca
     6061 aaagaaaagg cgatcatcaa aggactgcct tctgtagaaa cattaggttc tatgacagtg
     6121 atctgttcag ataagacagg gacattgaca aaaaatgaaa tgacagttgt ggatgtagca
     6181 gcaaaggaag aaactcgcgt actgtttatt atgaaaaatt gtcaggaatt aaagacgaaa
     6241 gaagaacaga aaacagaaaa tttaagtggc aatccgacag aacttgcact gattcgccac
     6301 accgaaacag ccgatctgcc tttgaaaaaa atagaagcaa aaattccgtt cagctctgaa
     6361 tacaaatata tggcaacgat gcatgcggag gaaaaaggag gggtgattta cgtcaaaggt
     6421 gcgccagaag tattattggc taaatcaact ctttcttctt cagaataaga agcatggggt
     6481 caaacagcca cagcgtttgc gaaaaaaggt cagcgtgttt taggatttgc ttacaaaaaa
     6541 gtagattcga aacaggaact cacgcatgag tctttaaccc aattaacttt tgcagggatt
     6601 gctgggttga ttgatccgcc aaaagaaagt gcagtcaaag ctgtaaagga atgccaacaa
     6661 gcaggaatct cagtgaaaat gattacgggg gatcacaaag atacagcaaa agcgatcgcg
     6721 gaacaaattg gcttaaaaca tactgctaaa gttttagaag ggatcgattt ggatcttatg
     6781 tctgaagaag agttgattcg acaagtacca aaagtcgacg tctttgctcg aacgacacca
     6841 gaacacaaac tgcgaatcgt gaaagctttg cagaaaaatg gagaaatcgt cggaatgacc
     6901 ggagacggcg taaatgatgc acctgctttg aaaagagccg atataggaat cgctatggga
     6961 atcaaaggta gtgaagtaag caaacaggca gctgatatgg ttttaggaga tgataatttc
     7021 catacgatcg caaaagccgt aaaagaaggt cggcgcattt tggataagtc tgctaagacg
     7081 ctaactcctt tttttgaaga aatggctcaa atagcatggc attatgctat tgatcaggag
     7141 ttaagagaaa gaattgctga gattgggcga aaagcggaaa aggcaatgct gattgctaca
     7201 aatggcatca atacccataa aggagcgatt tgggcaatgg ggctattgat cagtgtcatt
     7261 gcacaaaaaa tcagtatgga acagcctttt tctttatcga aaatacttga tgatgtcagc
     7321 aagcttgctg cttttcctga tacaaaatat cgtcataaaa aaaccactca tggtcatagg
     7381 gtgaagcaaa aatatggcgt aaatggtgca tatgaagaag cgatattagg gtatccgcat
     7441 atcaggcttg caatccaagc agctaaggag ctagataatg attcatatgt acagaaacaa
     7501 atgcatatgt tgttagtttt gatgagttcg ctagatgaca catgcgtatt gcatcgcagt
     7561 gatcagaaaa cgttagtgga aatgcaaaaa cttgctcatg ctgctagtca aagtgcttta
     7621 ccaaatcgtc catttacagc actgttgcac ttttgtcagg aaaaccaaat ctctcctgga
     7681 ggaagcgcag atctcttgtc tgcaagcttg ttcatgctga agatagagga attatggact
     7741 gccgacacat acatctgtcc gatgttactc aatgactaaa taagacaaaa ttgctaaaaa
     7801 aacagtttat gcatctggtt attgactctt ccttttagca aggctatgat aaaaatagtt
     7861 ttttgtaata aaagataacg aggaagacaa tgaaaaaatc aattatttag caatcatttt
     7921 gttagtgggc ctatgtttgt ttgctctgcc gacccaagga ttcgctgtcg cgggtggtca
     7981 tggtggaggt acatctagcg gtcgaagttc gggcagaggt tcatttggcg gtcgttcata
     8041 tgggggcagt tattttggca gtcgttctta cggcagagat tcttatgggt attatggtcc
     8101 aagtacaggt ccttctagtt atgagcaaac ggggtttctt gcattagctg ttttgtcagt
     8161 aggaggggtt tctgcttttt ctatcgtcaa gaataaaaga caaaagaaaa ggctgaaatc
     8221 ggagatattg tcccgcatgt caggaacggc taaagaaaag aaagcacagt ttttggaaat
     8281 ccaaaagata ttcatgaaga tccaatccgc ttgggaagaa gaaactcctg aaaaagccag
     8341 aagctgttat acagacaagc tgtttgccga acatcagaaa gttctgcaaa aaaacaaagc
     8401 agacggtgta agaaatcata ctaaaaaaat aactattcag gaagtaggaa attatcgaca
     8461 gatccacgaa aatagttttg ccatccgaat tgatttcaga tgctgtgatt attcagtcga
     8521 tcgaaaagat gggcgattga tctcaggaag taagacacaa cgacagtatt tttctcagat
     8581 atggtatttt gatttttctg agaaagaaaa aagatggcaa gccgatttta ttcaaccgat
     8641 tatgctggat tgacaggaaa tatttatgaa cggagttgtg acaaaagcct tgtcgcagct
     8701 cctttttctg aataaacggt gttccagaga tagcctatcg gcaataaggc acgattttgt
     8761 aaaatatggg aagcgatttt tcaaaatcgc ttcttatcgc tcaaggctga acatctctgt
     8821 cacagcttct ccactaaatg gtgtttaaaa gcttgcatct gcgataataa gaacaaccaa
     8881 acaaaaaata ggaagcacta ttttagttta gttgttctta ttacttgatg caggacagct
     8941 ttttcacaac ctcttttttg tatagatggg caaaattttt gtaataatag gcgtatgtct
     9001 ttttcgttga ggagatgaaa aagcatatgg cttatttttt gtttgctttg cttactttct
     9061 ttccacttca agtggcagaa atatctactt tttattatgt aagcggaacc atatacttga
     9121 aatcagaagg agatcatttc tatgaaaaca agaacaattg aaatacttca cagaatcatg
     9181 acagcccaat atccagtaaa agcaaaacat ctagcaagtg agttcaatgt cagtcagcgg
     9241 acgattcgtc aggaagttct tgaagcgaat atctggttga gcagtaaaaa gcttccggag
     9301 atccgtacaa ttcgcaacaa aggttttttg ttgaagctgg aaaaggaaaa gcaagctcga
     9361 ttagaaactg aaataaaaga tgtgaaagac ggctttttga atcggcgcga acgtattttt
     9421 gatctagttt tagccgttgc ttacagccga atcccgatcc atctaaacaa aaaagaagaa
     9481 gaatttcaaa tctctaaaag tacattagat gaagatatga gacggttacg agcaacactg
     9541 gtcagttatg ggatagagat cgtcagttat ggaaagcaag gactggttta taaaggttca
     9601 gaacgatcta tccgtacgat gatctatgat tttatcaatc agaatctggg aatgaccgac
     9661 ttctttatga atcaagatgc acaattaaca ccggctcaaa aaatctttct ccattatttt
     9721 cctttggagg aaatcaaaaa aatacaggag atctattcca ataagttgtt gaaacaagaa
     9781 gatgatattt ataaaaaaca aatcctgctt tttacattga tctggatcca gcgaatcagg
     9841 aaacatgaat ttatctctgc cattaattgg aaaaatacag aaataaaaga cagtagcttt
     9901 tctgcattta ttgaccacat gatcgaggaa tttcaattgg acaatgtccc gcaagtagaa
     9961 agaaattatc ttcgtttcac tattgagacg ttcaatgcta gagatatctc caattcaatc
    10021 gagtgggtac aagctcaatt actaacgatc caattgatcc aatttgtaga gaacgagaca
    10081 cacgtacctt tccatctaaa ggaagaagcc ttattcgaaa atctctataa gcacatggct
    10141 gcactgattg taagaatcaa gaatcatatc caagtcatga atccattgaa agaaaacatc
    10201 cgaacaaatt atttcccaat ctaccgcgca gtcactcatt ttgttcctac gatcgaagaa
    10261 gtaattggtg gcaagatact ggaggacgag atagcttttc ttgtcatcca cttttcgacg
    10321 atcgccagtg taatcaagcg agacttgaat tatgtttata aatcagtcgt tgtctgtaac
    10381 catgggatgg ctactggaaa tctgttagca gagaatctga aagaaaagtt tcctcagatc
    10441 gagattactg cagttttaag ttcaaaagaa atcgaattag tagataaatt ggatgtcaat
    10501 ctggttttca gcacctttca attgaattat cgaaataagc cgcttttagt tgtcgatccg
    10561 attttaactg agacgaatat cttggttata gaagactttt tgaaaagaaa tgaagaacta
    10621 cagcggttag agcctaacag tcatgaatcg acggagttat tcactcaaat aatagatgtc
    10681 atgaaagaaa gtggtggccg agtagatcga atcatctatg gcaagttaga aaaattattc
    10741 gaaacaaaca atttagaaat caacaaaagg gagatccagc ctatgttaaa agatattttg
    10801 acggacagca atatcatgat caaagaaaaa gccactactt gggaggaatc gattgaacta
    10861 gctgctagac cattggttaa agaaaacatt gtcgaagagc gctatgtcga agcgatgatt
    10921 cgtgcagtga atgattacgg accgtatatc gtgattggca aacacatggc actagcccat
    10981 gcccgaccag aagatggggt caataaatta ggagtcagcg tagctacgat tgagcaacca
    11041 atcgatttcg gtaatcctga aatggatcca gtaaagatta ttttttgttt ggcagcagta
    11101 gactcctact cccatctgaa catcatgaaa gaactaattg aattgatcaa caacgaagag
    11161 aagttggatc gattgattga atgcacaaat atcgaagatt tcaaacaact gcttttttca
    11221 gtagttgaga aataataaat gatacggggg agaacaaaga tgaacaaatt gaacatttta
    11281 ttcgtatgcg gcgcaggttt aggaagtagt tttgcagcac agatgtctgc tgaggatgtt
    11341 ttgaacaaat taggagtaga agccaaactg gaccattgtg atatttcttc cgcagtttcc
    11401 atgaaaccag atgtgatcat cacagcagaa aattttcgtt cgcagttcga aaagttcgca
    11461 gttgatccta aaacggcgat gatctatttg aaaaatattg tttcaaaaaa tgaaattgaa
    11521 gaaaaaatca ccccagttct gcaagataag ggagttatct aaatacaaaa agaaaagagg
    11581 gtaaaaacat gagcgttatc aattttatca tcgaaaatat tttgactcag gcatcgatca
    11641 ccattgcatt gattgctatg ttgggtctac tactgcagaa gaaatcagca ggacaagtca
    11701 tctctggcac gttaaagaca ctcctaggat ttcaagtgtt gagtgccggt tcgagcatca
    11761 tcgttggaag tttaacttat ttcgggaaaa tctttactga aggatttcat atgcaaggga
    11821 tcatcccttc tattgaatct atcaatggtc aagcaatgaa tgatttgggt ttaggtagag
    11881 atattgcact gacgttctta gccatttttg tattcaatat tattttggct cgtttcacaa
    11941 aatggaagta cattttttta actggtcaag caattttatg gatggcaacg atgacgacag
    12001 tctttggcta ttttgccggt ctgcgaggga tcgtattgat cttggtcggt ggttttatcg
    12061 gagcttgttt cgccattgct atgccggcag tagcgcaacc gatcattcgt aaaatcactg
    12121 gatcaaatga tattgcttta ggtcattttt gtacgatcgg gtacttattt gaagccggtg
    12181 ttgctaagct ttttggtgag aaaggcgaaa ataaaaagtc gatcgaagat atcaagttgc
    12241 cgacacattt cgaatttctg caagatactt atttgtctgt catggtcgtg atggtcccgc
    12301 tatatatcat cactgtttta tttgcaggcg aaccattcgc ttctgaatta tcaggagatc
    12361 aaaactatat catgtttgcc tttttacaag ccatccaatt cgtagtgggt gtgtatgtat
    12421 tgctagctgg tgtacgtcta ctgttaggtg aaatcgtgcc ggcatttcga gggatagcta
    12481 tgaaattagt tcctgatgcg atccctgcct tagattgtcc agttttcttt ccttatagcc
    12541 caaatgctgt cattttaggt ttcattacga caacgattgg tacgattatt gccatgttta
    12601 ctttgcccat gttcggctta gcgatgattt tacctggtat gctaaccaat ttctttgccg
    12661 gagggacagc agggatattt ggcaacgcag ttggcggacg ccgaggagcg atcattggcg
    12721 gtattgctca tggtttcttt attacattat tgcccgcttt gctagtaacg atctttaatt
    12781 cgatgggatt caacaatgct acagcaaccg atgtagatac agtagccgca gcactcttat
    12841 atgcctggat cttaagccca gttttgaaag cattttaatt gaaagtaggg aaaatgatgt
    12901 ttggattttt taagaaaaaa gaaaaagaac cgaaaaaagt tctggcaaaa gaagaaaaag
    12961 ccctatcgcc agaagcgacc gaaaagattc aatcagagat tacacaattg aaacaagaga
    13021 tttccactac ccaagataag cataaattag cgaaacttta tgaacaagtt ggacttaaat
    13081 tttctgaatt gtatgtgaac gatcaagcca tccaatattt agaaaagagt ttggaaaata
    13141 aacagacgat cggagatggg tataaaaaac tgatgagcct ctataaccag aagcgtgcag
    13201 atgctgctcg tgctggtgat gatcaaggga tcgattacta catgggcaaa atggatgaga
    13261 tgcgacaaat tgcaaaacaa gtaacgatca aaggaaacaa ataaaaagat aaggtggcaa
    13321 taaaagatgt atacaacatt gaaagaagta acaaaaacag cagaagagtt gaattttaca
    13381 gttggagctt ttaatacgca taacctagaa atgcttcctg ctatgctagg cgcagcaaaa
    13441 gaaatgggtt cgccgatcat tattcaaaca agtgtcgata ccgctaaata tatcggcatg
    13501 catgtgttag tcaatgtaat gacagctatt gcagaagaag aaatggttga tgcagtcttg
    13561 cacttagacc acgcccgaga ctttgatgat atcaaagaag caatcgacag cggatatact
    13621 tctgtcatgt atgacgggtc ccatctgcct ttcaaagaaa atatcctgaa gacaaaagca
    13681 gtagtagaat atgcccatgc gcacggtgtt tccgtagaag gagaacttgg aacaatcggc
    13741 ggtacagaag aaggaatcca tgtcgctgaa aatgacaagg tatatacaag accggaagac
    13801 gcagtgaaat ttgtagaagc tacaggcgtg gatgcattag ctattgctat cggtacgaat
    13861 catggacaat tcaaatcaaa aacagaagta aacattccct tgctgaaaga aatcgatgca
    13921 gtagttgatg tccccctagt tatccatgga ggaacaggtg tgaaagaaga agattatcct
    13981 gaattgatca ataacgggat ccgcaaattc aatgttggaa cggagctttt agtcaactgg
    14041 acgaaagtag ctaaagaaac ctttagtgaa acagaaatca acaagtctct tcgccataat
    14101 gtgattccag caaatgaagc agtaaaagaa attgtcaagc ataagattgg actgtttatg
    14161 aacttggaaa gtccaattaa cttaggaaat aaatgagatg aaaaagtatt tcattcttct
    14221 ggctggtagt cctggtacag gaaaaacata tcttatgaat aaactacggg aacgatttcc
    14281 agatatgtat gccatcacgc tcgatgaaat caaggaatac tatgcagaat ccatcgggtt
    14341 taacaatttg gaagaacggg cggaacaaga aaggacgaaa gtttatcctt tctattacaa
    14401 agcacttgat ttgtatatgg aagcaggaaa gaaagtgatt gtgtccgagt atccttttag
    14461 tgataagcaa aaagcacgac tccaagaatt agcagataga tacggatatc aggtgatcac
    14521 gattcgccta acagcagact ttgaagtgtt atggcaacgt cggtacaaac gtgatcgtga
    14581 atcagatcgt catttgagct atatcatgga tcattatcac tatggtgacg tattggaaga
    14641 tcgaacgaaa gggaccaatc atattacgaa agaagcattc aaaaagatca tcgccgaccg
    14701 aaaatatgac aaattccagt taggaacact atatgaattt gatgtaacag actatactca
    14761 agtggattac actccactat tggataaatt agaaaaagaa atcaatgaca ataagtaaca
    14821 aagaaatggc ttaatatcga aaaattggct tttgaacgct gtttaccgag aggtcgtgag
    14881 cctgtcgttc agctccgagt cacaaggagc aatttcaaaa aaacagctt
//
LOCUS       DACEUZ010000066        14872 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.066, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000066 DACEUZ010000000
VERSION     DACEUZ010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14872
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.066"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            149..838
                     /locus_tag="IXG44_001657"
     CDS             149..838
                     /locus_tag="IXG44_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1658380.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PKDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            844..1800
                     /locus_tag="IXG44_001658"
     CDS             844..1800
                     /locus_tag="IXG44_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HAQ1658381.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            1844..2356
                     /locus_tag="IXG44_001659"
     CDS             1844..2356
                     /locus_tag="IXG44_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1658382.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSGVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(2612..3160)
                     /locus_tag="IXG44_001660"
     CDS             complement(2612..3160)
                     /locus_tag="IXG44_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ1658383.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(3249..4430)
                     /locus_tag="IXG44_001661"
     CDS             complement(3249..4430)
                     /locus_tag="IXG44_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HAQ1658384.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(4666..6174)
                     /locus_tag="IXG44_001662"
     CDS             complement(4666..6174)
                     /locus_tag="IXG44_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HAQ1658385.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATCLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            6333..6524
                     /locus_tag="IXG44_001663"
     CDS             6333..6524
                     /locus_tag="IXG44_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1658386.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            6727..7644
                     /locus_tag="IXG44_001664"
     CDS             6727..7644
                     /locus_tag="IXG44_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HAQ1658387.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            7637..8710
                     /locus_tag="IXG44_001665"
     CDS             7637..8710
                     /locus_tag="IXG44_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1658388.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            8858..9376
                     /locus_tag="IXG44_001666"
     CDS             8858..9376
                     /locus_tag="IXG44_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1658389.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            9357..9749
                     /locus_tag="IXG44_001667"
     CDS             9357..9749
                     /locus_tag="IXG44_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HAQ1658390.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVVFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            9825..10760
                     /locus_tag="IXG44_001668"
     CDS             9825..10760
                     /locus_tag="IXG44_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HAQ1658391.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            10893..11672
                     /locus_tag="IXG44_001669"
     CDS             10893..11672
                     /locus_tag="IXG44_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HAQ1658392.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            11665..11982
                     /locus_tag="IXG44_001670"
     CDS             11665..11982
                     /locus_tag="IXG44_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAQ1658393.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            12226..13836
                     /locus_tag="IXG44_001671"
     CDS             12226..13836
                     /locus_tag="IXG44_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ1658394.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            13993..14841
                     /locus_tag="IXG44_001672"
     CDS             13993..14841
                     /locus_tag="IXG44_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ1658395.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
BASE COUNT         4803 a         2663 c         2926 g         4480 t
ORIGIN      
        1 gttctatcca tcagtcaatc tgaaaaatag aaatggtgct agcgtggctg gcaccatttc
       61 tattttttgt ttcttgtata ttctgcctag tattttaaaa ccatttcatg taaaatgtaa
      121 atcgaaaata tgttcggagg tagaaaacat gaagaccccg ctaaccatcg aaatagaatc
      181 ctcgttatac caatattgca tcgagcaagg cggcttggtt gtagaagaag tcacgatgcc
      241 tgatgaccaa ggaattgtag atacgcttag ctgcttgaca aaatttgatg gaagccgaga
      301 atggcgatgc tatgagctga aagtgacaaa agcagatttt cgctctacag ccaaactgtc
      361 atttgttgga cattataatt attttgtttt accaagtaaa ctatatgaag aagtaaaaac
      421 tgaaatccct acaggaatag gtgttttagt gtatcgtccg tacacggttg aagaggagat
      481 gcctgcctct ggtacatttc tgattgcgaa aaaagcagca aagaaagagc ttgctgtacc
      541 ggaagaagct ttgacaaacc gcttcatggc ttctttattc agggaagtaa gaaaagcaaa
      601 gcaaatggag cgaggcgtaa aatattttcc aaaagatcaa ttgtacaaag aattgaaaag
      661 acgttcagaa aatcaagatc cattttctaa cgaacgatat tatgaacggt ttttagagga
      721 aatagctagt caggcaatca atgagttgca agaagaagtt atcgcgcttc agcatgatta
      781 tgaatatttg aggcaacaac agcagatccg tcggttgcct acagagccat tggagtgaaa
      841 aatatgtact atccttattt aagaggaaaa caatttgacc tattagcatt aaaagaagca
      901 ctgtcgcgag gattgctctc caataaaatc caaccagtga tcgaaccagt acgtgattcc
      961 gctacgctta aaaatgtgat cgaattattt caaaaaaagc accatccaat cgctgtgatc
     1021 caaaatcccc aagtaggaca attcaaatta ttcgatcagc atgtgcacag ttggacagtc
     1081 aaagaaaata gctctgtcgt ccctgcacag atcttaacgc cagaaaatct cagtgaagta
     1141 ctggattcac ctccagcgtt tcttgttttc gatggacagc actacccaaa agatacagag
     1201 gtgtggaaac agttggcagg agttgattcg aaatttttga taccagatac tagtcgcttt
     1261 cgcatctggc tgcccgaaaa caaaattgtg atccgtgact cttttcaaac gagaaagcat
     1321 gttgaaagtt atgcagataa aacagatgac ttttttagtg atgactatct ttttttccac
     1381 gcagacggat atattggatt ttctgatttc acgatcgaag gtagccgtta tttcgataaa
     1441 gggttcccta gccgagcgct tgctatccat ctaacgtata ttgatgctta cggcaatata
     1501 cgcgtgaaac attttgtctc tgacagtaac gactctgcta aagaccaagc attgaagttt
     1561 cttgaagcag gagataaaat gaaaaaatgg atcatgcgtc accatcatca actcttgatt
     1621 acttcaggaa tgattgaatt attggctctt taccagcaac aaaaattccc tggactggga
     1681 gtgttgaaaa agtggtcact tatgcatcat ttggaactta ttagtcacct gctagatcat
     1741 ccaaaagatt ggcttaaata ttattcaaaa gttcaggaac tatatgaagt gatccaatga
     1801 ttagataatg taaatgaaaa aggaatagaa aagaggaaac tggatgtata ttcggcaaac
     1861 aaggaaagaa gaagtagaag aagtggtaga aatcattaat gccgcagcgg atttcattgc
     1921 ttcgcaaggc tctcctcaat ggcaaaacgg gcagaaacca acaaaagaaa cgattttgaa
     1981 cgatattgaa agaaaagaaa gctatgtatt aatagtggaa gatcaaattg ctggaacagc
     2041 tgcattagtc tcaggcgtgg atcccgttta cacaaaaata gatggtagtt ggaaagaatc
     2101 aactggtcct tatttatcga ttcatcgtgt cgctttagca gaaagtactc gaggaaaaaa
     2161 actagcaaaa ccgttcttag aacatttgtt gacgattgca gccagcaaaa atatccaaga
     2221 tatccgtatc gatacttatc cagctaatga accgatggaa aagaccatct attctgctgg
     2281 attcacttat caagggatga ttgaatttcc ttttgaacac ggcgaaagaa aagcatatca
     2341 aataatcatc aattgactat aaaatcatct gagcaaagat aaagacatta acactactga
     2401 aaaaaacaat catatgatag ccaagaggtt gtaacagaag tggccagctt caaaaaacaa
     2461 gaaagaattt ccgaaaatgc ttctcaaatt tttgtggaat tttagcgtat tttacgaaat
     2521 agctgctttc tgaaactccc ttcatcgaga ggtcgtgaca tagcttttgt cacgatctct
     2581 cggcttatcc tacgagtatt attcgataca tttatatcaa taatcctttt tttattttat
     2641 ctgcagcttc ttggaacgct ccatcgtttg acacgactaa aagtgagggg atagctttca
     2701 aacgtttcaa ctgtgctaaa tcttctacat aaaccgccag tctggctgaa atctgttcag
     2761 atgaatctcc acgttttttc aggcgcgatt gcaatgtttc tttagagatc gtaataaaga
     2821 taggacggac taccgtcccg aaatactcat ataaatgcca aaaaccattt gctgtcaaaa
     2881 cggcgtaaca atttcctact gtcagtttct ttttaatttc tgctttactg gaaccatatt
     2941 tatgttgagc atattggtcc cactctgcaa actcatttcc cttgatcatc tgattgaatg
     3001 tattgtcaga aacaaaataa taatcctctt gatctttttc cccaggtctt ggtgatcgat
     3061 tcgtgtaagt aatgatcttt tgttccggct gaaaaatagc ttctgcgagt gaagtttttc
     3121 cagaaccaga aggaccgata aatacatagc aataattcat tgtcatttct cctttgatga
     3181 ttgaacaagt gaaaaagctt tggaaaatat ttccaaagct tttaaaagta actttgcttt
     3241 tttgtatttt ataccagcaa gccagtcaca atagcactta aaaagctgac taaagtcgct
     3301 gttagcaaaa ttttcaatcc gtgttttgcg acaagatttc ctttttcttc atttaatccc
     3361 ttcatcgcac cagaaatgat cccgatagaa gagaagttgg caaatgaaac gaggaagaca
     3421 gatataattg ctgttgtacg ctcagtaaaa tggaaattac catttgctaa ttctgtcata
     3481 gcaacaaatt catttgtaac cagtttagtc gccatgatac taccggcatc cactgcttct
     3541 ttccatggga ttccgctgat gaaggcaaga ggggcaaaga tgtaacctag gatctcttgg
     3601 aaagtgatgc cgaatatccc gtggaagata gcattgatca tggcaatcaa agcaacaaaa
     3661 ccgataagca tagcagcaac cgtgatcgcc acatggaaac catcaagaat gtattctccc
     3721 aacatttgga aaaatgtctg ttctttgttt tcttcaatga tcagctcatc ttctttttct
     3781 tctaatacat agggatttac aattgaagcc agaatataac cgccaaataa gttcaaaaca
     3841 agggcagtga tcacatattt agaatcgatc agtgccatat aagatccgac gatcgacata
     3901 gaaactgtag acatagccga tacactcatg gtgaacaaac gtttctcaga taggaagggt
     3961 aattcctttt ttatggaaat aaaaacttcc gattgtccta aaatagcaga agctactcca
     4021 ttataagatt ccaatttgcc catcccgttg attttactta atcctagacc aacagctttc
     4081 ataaataatg gaagaatctt gatatagcgt agaataccga taatagcaga aataaataca
     4141 attggcatca atacggaaag aaagaaaggt gcacttcctt tatttgctac accggcaaaa
     4201 acaaagtcaa caccttgccc agcaaaagca agaagatgat caaacatttt tgccaataca
     4261 gaaacaaccg ctgtaccaaa agttgtacgt aataatacga aaccaagaac aagctgtaaa
     4321 acgaacatca caattagtgg tttcaatttg attgcttttt tgttactgct gatcaaaaaa
     4381 gcaagcccta atgtgacaaa taaaccaata atacctaata aataactcaa ataatcactc
     4441 ctattccaaa ttccgtttta ttttacctgt tttgcaaata aaatactaat ctttagtgaa
     4501 attttcatta cttttttaga gtgaaacgca ttcattttcc attagaaaat aaagcgaaca
     4561 aaataaaagg tttaaaaagt gtaacgtctt agtaaagtct ataaaaaaac gaaactatcg
     4621 attaagctaa tagtttcgtt ttcaacattt ataagtaaac tttttctatt ggtaatatag
     4681 attttcggaa gggtagacag gtgcgcaggt aatatcgatt cctagttttt tcaatgtttg
     4741 ttcatcattt ttattgacca tcacagtaga gtgggcttgt gctccctcaa gttcagctag
     4801 tttatcataa gcaacttgag cgattggatt cgttactgca ctgattgata aagcgatcaa
     4861 gacttcattg atgcttagtg catccaaaga ggaatgaagt gtctttgatt tcaatccttg
     4921 tattgtttca agaactaatg gtgacaataa aggaatgtca tctgcgatat gggctaaatg
     4981 ttttactgca tttaagataa ctgccgctga cgcatccatc aatgaacttg tacgcccggt
     5041 aatgatgact tcatctggta attcgattgc aatcacagca gaaggatctt ttgtaggact
     5101 ttgcgcttga atcttatctg catagtcatg ggcagggact accacgtttc ggtcttcagg
     5161 acgtaaaccg acttcttcca taatcagttt gattcgatta actgcttctt cgtcgatcaa
     5221 tccttttttg aagtcgcatt ctgtttggaa ataacgacgg atgatttctt gtttagaggc
     5281 ttcttggaca acggtatcat ccacgatccc aaatcctaca cgattcacac ccatgtcagt
     5341 aggagattga taaacagatt ctttaccagt aattttttca ataatacgtt tgattacagg
     5401 aaatgtctca acatcccgat tatagttcac agctacttta ttgtaggcat caaagtggaa
     5461 ggaatcgatc atattgacat cttttagatc gactgtggct gcttcataag caatgttcag
     5521 cggatgtttc aacggaacat tccatactgg gaaagtttcg aacttggagt aaccagctgc
     5581 atgtcctttt tggctttcat gatacagttg gttcaagcaa gtagcgagct ttccacttcc
     5641 tgggccagga gctgtcacta caacgattgg tttagttgtt tcaatatact cattttttcc
     5701 aaaaccatct tctgagacga ttttttctac gttgactgga tagtcttcga tttctgagtg
     5761 tttatagacc ttgatgtttc gtctttctag tttattgata aagactttcg tggcaggctg
     5821 tccactatag cgagtgataa cgacactgtt gattaataag ccataaccac gtaattcatc
     5881 gattaaccgc aagatatcca tatcataagt aatcccataa tcaccacgga tcttattacg
     5941 ttcaatatct ccagcataga cgcaaatcag tatttcagct tgatctttca atttttgcag
     6001 caatttgatt ttcgcatctt cttcaaatcc tggaagtaca cgtttcgcat gcttgtcgtc
     6061 aactaacttc ccaccaaatt ctaaatataa tttgtcatag tggttcactc gttccaaaat
     6121 atacgctgac tgctcttcaa tatatttttc tgaatcaaaa ccaatttttt tcactaggtt
     6181 ccctccttaa gtactaaagc attttttcat ttttttgagg attcgtcaac tggaagagcg
     6241 aaattcctca aaaaaacaaa gaaaatggtt taaaccaact tgtctagata gttattcatt
     6301 ggtatgatag tttttgataa ggaaggtgct acatgaaaaa aagacagaaa aagaaaaacg
     6361 catataaaca ctatatccgt tcgattttta ccggttatga aagaatgctt gaagatcccg
     6421 aattggaaca attgacgttt acttatctaa acgaagaaac gcagctaact cgtgatgagc
     6481 atcagcgcat tcatttcaca acaagagatc tgcctagtaa ataaaaaata agataaaggt
     6541 catcggattc gattctgtcc tatcagatgg aaagactagt tgatcgaaaa aaaggctgta
     6601 ccaaattttg gtgcagcctg ttttatggag cattgcccgc tcttttttat aacaattttt
     6661 tacagaaaat ggtactattg aagtattaat aagtgaaaaa cagatgagta agaggaggca
     6721 agtagtatga aagagccaga tacaaatgca aattatgagc cagacgaact agaggagacg
     6781 aacgcggatc aggatacagg acaagaatcg tcttcttttt acgatatcaa agaaaaagca
     6841 agcatgaatc tttcccaatt attagaagac ttacaggctt ttgaaaaagc tgtccgcgaa
     6901 gaagatatcc ctgagatcta ccgtttatac aatggccgat tgaatgaaga attaaaaaga
     6961 agctcaaatg aaaatcacga gatcgatcaa ttgctgatgc gtaagatcca tgatcgtttt
     7021 ttacaggaat ttccttttat ggaacaagta gaaaacatct cgccaacgat gagctattac
     7081 aaaatcggca cttattacca tgatcggccc acggtcggta ttgatgcaag tctgccagaa
     7141 atttttgttt tgcctaagat cgatgaagag tgggagaaat attcccaacc ggagtcaacg
     7201 gactatgaca aaaaaatcaa tgagctagac gcaaaggtga tcactgctca gacagaaatt
     7261 gaacgtttag atgaacaaat aaaagaaatc aatcgacaaa tgactgattt gaacgacaat
     7321 aaaggattct tgaatcgtaa aaaaatcgag gaagaaattc aagaattaga gaagaagaag
     7381 caagtactat ctaatgaaaa attaggctgg cttccttata ttgagacgcc tgaaacaatc
     7441 cagcagcaaa aagaagcatt gaagcaagaa gcaagagcag accagcttcg tgcagccatc
     7501 gtagaaaaag aacaaaggca aatcagacgc tatttcggta gcaaagaggg attcggtcaa
     7561 gcaatccatg aattcttgat gaattatctt ggcaatgaga atcctaataa tcaatctaat
     7621 gaaggaggtt ccgagtatga atgaacacga tgaagcagaa tggacaaaaa atgaagaaca
     7681 aacagaagat catgaagaaa cgaacacttc attggaagaa caaacaagta atcctctgga
     7741 tgacacagaa gaaatcccta tgctagagga aacaaaaata catgaaaaag aaaaagtaac
     7801 tccgacgaat acgaatactg cagaaaacac ccatgcttac gaaataactg aaaatcccgg
     7861 gtatttagaa ggaaaagagc aagatcatca agatatcgtc agtttgattg aaacagcgga
     7921 agcagaatta gcggatttac gtatgagaaa agatttgatc gaatcagatt ttccacaatt
     7981 attagaatca tatgatcgtt ttatattgaa tgaagaacca atctccacga tcggaaagac
     8041 aagcctgatc gaatacttca cgtatgaatt actgaaaccg ttgaatgata ttggtctgga
     8101 acggtcaaat tcagcttatg acacatggaa aacaagacat ttttcgatca attatacgtt
     8161 gaatggacaa aacgaattag tcttttcctt tgtattaccg accatcgatc aacgatacca
     8221 agtggaatcg atgagtctcc taaacgtatc accagagaca atggaaatca atgtccaaga
     8281 tgaccgggtg ctttctttga tccgacactg gtcagtggat cgagtattct ctgcagggca
     8341 gatcacgata ttcaatcaca aattgaatca attattagcc catgcaagaa cacttggttt
     8401 taccgttaat cagacattac tagacaatac gaagccactt cgtttgaatc ttcagagcga
     8461 atttgaattg acgaatgaag tattggatga tatttttatc gtggcaatga aaaatccatc
     8521 ctacgatatg gaaaagatca acgaaactac ttatcaagta cgattagata aaggacagtc
     8581 tttaacgata acaacaaacg aaaaagatca aacagtcttg tcgatctcat ctggagacta
     8641 cccacgatct gtcattgatt tctttatcaa ttacgaattc ttggtcccat taatggtgag
     8701 aaagtcctga ttggataagt tggattacca gatgtcattc agataattct gttccaaagg
     8761 acttgcggca gttatccaga ataattgtca caagtctttt ctgtatgtac taaatggacg
     8821 tgaataactt gctgatgaag gagggatttc attaggaatg tccacaaata aaatagcaca
     8881 gcttagctta ttgtctgctg cttgtgtagc aggtaggatc gcttttcaat ttattccgaa
     8941 tttccagcca atgactgcta tttttttatt tatagtactt tacttaagct taaaagatgc
     9001 gttaattgtc atgagtcttt ctattgcgat cagcagtttt tattttggtg ttggaccatg
     9061 ggtcatcggc caatggatca gctatactgt tgttctgagt ttgttttcaa tcgtctgctt
     9121 gagaaaaaca gtgcaaagga atctatggtt aaagggagtc tgtttttttc tggcgggcat
     9181 ggtttacgga ataggtatga ccgtttttga tacgctgtta tatcgtttgc ctcaaccttg
     9241 gatctattac cttcaagggg tgtcgtttga tttgatgcac agtataggca atgttgtgtt
     9301 ctttcttgtt tttctgccag tcgtcaaacg tttttacaat cattcaggag gaaaaaatga
     9361 aaaaaataat ttatagtttg cttgcaatga gtgtcagtat actcattttg acaagttgca
     9421 gtacaaccta tacacaatcg gcttccaacc aaaaggagga agtaaaaaca tcgcaaaaag
     9481 caacaatcac gttacaagaa aatggtcaaa acttttcgga aaaagaagtg gtcttcaaaa
     9541 aaggcgatga tttactgagt attttgaaac gaaattttga agtcaaagaa gataacggct
     9601 tcatcacttc gcttgaaggg cactcacagg atgagaaaaa tcaactctat tggatgttta
     9661 ctgtggacgg gaaaagtgct actactggtg ccaaagagat caaattgaat gatggacaat
     9721 cggtcatttt caatttgtct aaactataag tttcataaaa aactgttaaa gggcgaatat
     9781 tgccataatt cagtgagttc agttgatgaa agaaggaaaa taacatggaa aattttaaaa
     9841 aaagcggaat gttttccgta attgctgctc taggggctaa tattctagtt gctatcagca
     9901 aatttgtcgg ctatgcaatc agtggcagtg ccgccatgct aaacgaaagc atccacagta
     9961 tcgttgattg cagcaatcag attttgctgt tattcggtga taaacaagcc aataaaggac
    10021 agagcgagtt gcatcaattt ggtgaaggac gagcaaaata ctttttcagt acaatcgtcg
    10081 caatgatgct tttcttcggc ggtggggcac ttggggtgat ggaagctttt gaaaagttga
    10141 cgcatccatc ccatgaagtg ggtaatccga tcattgttat agcaatactg ttatttggtt
    10201 tggtaatcga aggaagttcg ttacgcgtgg caatgaaaga aatcaaagaa ttgaatactg
    10261 aaaaactttc cacgatgcgc tttttgcgcg aaagtcgaca cagtgaaata ctgattattt
    10321 ttacggaaga cttgtgtgct gtaatcggtt tggtcttggc tttagcagga acaatattga
    10381 cttatgcaac cgggattgct gcatttgatg cgatcagcgg attgctgatc ggtttcttgc
    10441 tgatgggagc cgccattttc ctagcaaaag aattttatag tctgatcatc ggtgaaagcg
    10501 tgacagcaag cgatttatca aaaatcaagt tagcttttga acgtcctgag atcagtcggc
    10561 tgatcgatat aaaaacagtt catttaagtc caacggagat tttagttgca gcaaaagcag
    10621 atatcgttgg taacaaagag aatgaagctt actcggtcat caatgatatt gaaaaaaata
    10681 tgcgtcagtc tcttcctgac aagaagatgt atatatacat tgaaacagat aaatatgacc
    10741 caaattattc tcgtaaataa aacgtttatt catgaattga agcaaaaatg agaaaaatat
    10801 aatcttgttt ttaaataaaa cggctacaat ttagcgaatt tatgttacaa tgagtccgct
    10861 gtttatttgt tgtaattaaa ggagatattt taatgaacaa aaagaaaagt gcgccatggc
    10921 ttcaagcttt gcatgcagtg atgccacttt gtataagcta tataccagta ggattagctt
    10981 gcggagtatt gttgcaaaaa gttggatttg atcctctctt atcaggactg ttatcgattc
    11041 tggtcttttc cggtggcgcg cagtttttag ttgcatctat gcttacgacg caagcatcat
    11101 ttgcaacgac attattgatg gttttctttt tagaattacg ctatacatta ttaggagcaa
    11161 gtcttgccgg ttttatgaaa aaagaaaagc ggacgttttt ggctgtcttt tctcaatcac
    11221 tgaatgatga aaactatgcg gtgaactact taaagttttc aactgaccct tcctggaata
    11281 aaagaaaggc tttatatgtc aattggttct caatgactgc atgggccggt agcaatatga
    11341 tcggtaattt tttcggctcg gtgattcgag tagatgctga ccttgtccat tttgctttga
    11401 cggccatgtt cattttcatg tttatcatgc agatgaaaag cgcactactg atctttacag
    11461 gtttattctc aggcgtatta ggggtcgtat tcatggtatt attccaaaat actttcggat
    11521 tgattgcagc aacagtcatt gcttcatttg ccggatttat gctggagaag gcattcaaaa
    11581 aagaaaaagc taagaaacta agagaccgcg gtaaagatgt caatgagccg ctcttcaact
    11641 tcccggaaca ggaggccaat caccatgact aaactatatt tgctactttt agttgcttgt
    11701 ctgttcatcg tggcatatat cccgcgcttg ttcccgatgc tttattttac tcatcgaaaa
    11761 gtgccatcct ggttcagtga ctggatgaaa tatgtaccgg tggcattatt tgcggcgtta
    11821 gcttttaaag atgttttcat tactcatgaa catctggata ttgcttggaa tatcaaaata
    11881 gctgcaatga ttctagtagc aggcgtggct tacaaaacac gttcgatggc gttgtcagtt
    11941 ttgacaggac ttgcgtctgt ctttttgctg tctatgcttt aaactgtttt attttattaa
    12001 aaacagagat tgcgacataa gttgttcaaa ctgatctatc cgaactgcat ttgcaacagt
    12061 caattctgga tgcaagttta ttcgtaggga taaaaaagat tagtgttggc ttatgatgca
    12121 agctcttttt tattttagga attagagagg gatttgtcct tctatgattt tttgctataa
    12181 tagggaagat gtttatttta tgaaagaata gttgagggaa tttaaatgaa taaaaaattg
    12241 atgcaaggaa cattttggct aacctttgct aatcttctat gtaaagtttt aggggtagtc
    12301 tatttgattc cctggctaag tatgatgggg aacaatcaag atggtatgct tgctacgaca
    12361 ttgtataatg ttggctatct tccttatgga ttgttcctga tgcttggtac agtcggattt
    12421 ccaaatgcga ttgcaaaaaa agtagctgta gccacaaaag aaggagataa cgctgcttgt
    12481 cggctgattt ttagaagtac gatcaatatc atgtttgtca tcggtatcgc ctctgctgca
    12541 ctgatgtatt tatttgctcc tttactagcc ggaatcagtc cgattgctaa cgttaataat
    12601 gggatactag ctatcagaag tttatgccca tctttgattg ctatcccaat tcttagtgca
    12661 atgagaggat atttccaagg aaaaaatgat ctgcggcctt atggaacttc tttgattatc
    12721 gaacaggctg tgcgagtgat cgttatcttg gctggtactt attacttgcg cgtattgact
    12781 gacggaacca tattagaagc tgtattgatc agtacagtcg cttctttctt cggtgggtta
    12841 gctgcgatta tccatatgta tatcgtcgga cgcagaaaag acttcttcga acttaaagat
    12901 tttttgattt ccagccgtta tttcgaaaga gaaaaccgat ctgcctctgt ctctatcatt
    12961 cgggaaacac taccgtttat ttttgtcggc tcagtcatta ctatcgttca aatgatcgac
    13021 caagtaacga tgaaaccact gcttcacttt ttccgtccag agatcgcaga tcagcaactg
    13081 gaattcttat tcagccgtgc ttctgtcaat ccaaataaat tgacattgat cctgatctcc
    13141 atggtaggaa cagtagctat aagcagcttg ccaatattaa gcacgatgaa gaaaaaagat
    13201 cggatacaaa tcgaaaaaac agtaggagat agtttttcga ttgcgttatt gattcttctt
    13261 ccttcattga ctggaatgtc tttattagct ggaccactat acacattgtt ttttggctat
    13321 gatcctgaaa gtgtcggata tttccaaatg gctttgctag cttctttgtt tttctcttta
    13381 ttcacgatcc tatctacgat gcttcaatct ttgaatcacc atcgttttgc gatccgatta
    13441 actgtagaag caattatcct gaaagtcatc ttccaagtaa tcggattagg attagttggc
    13501 ggatacggga tgagcttatc aaatggctta gcattcggta ttgtctttgt tcgcggctat
    13561 cactttatgt gcaaagaata ccgaatagca cctttagcaa agatcagcaa ttttttctta
    13621 aaaactttcc gctccaccct gattatgctt gcagtatgtt ttgccgtttt ctttttgctg
    13681 aatcagcgat tgtcaatgga atcaaaaagc tatgcaatga tttattgtgt ggctgtagga
    13741 agtattggcg gcttagtatt tttattttct caattcggaa aaaatgggtt tcgaatgttg
    13801 aaaaatgtta aacatcatag cagaagcaaa gaataggtta actaatcgtt ttttgctgtc
    13861 acttgaaaaa cggcaactcg tatgattacg cttgtcgttt ttctattttc caattttagt
    13921 ttcatatcta atcttactta tgatatagtt ataaggtctg gaaaaattct ttgaaacgag
    13981 gaaattaaac acatgaaact tgcagtagtt acagatagtt cggcatatct gccggaacgt
    14041 atcaagaacc atcccgactt attcgttatt ccaatccctt tgattatgga tgggaaaatt
    14101 tataacgaag gaatcgatat cgaagcggat gaatattatg gacttttaaa aaacagtaaa
    14161 gattttccta ctacttcaca acctgtcgta ggggaagttt tgggtttata tgaggaattg
    14221 aaagagaaag ggtatgacac gattttaagt atccatcttt cttcagggat ttctggcttt
    14281 gtcaacacat tatttgcgat gaaagatgac atcagcggag taacggtcat tccttatgac
    14341 tctaagatca ctagtatgcc gatgggacac atggtagaag ctgccttaga cttaaatgat
    14401 gaaggtcagt cccttgatga aatacttgca catatcgatc gaatccgaga taatacctat
    14461 gcttatctga ttgtagatga tttgaacaat ttagtacgag gaggacgttt gacaaatggt
    14521 gcagcgctga ttggcagctt attaaaaatc aaaccgatcc tgacattttc tgaagggaaa
    14581 attgttttat tcgaaaaaat ccgttcaagt aaaaaagcat ttgctcgagc tgaagaaatt
    14641 attggtagaa gaaataacga aatacaaccg ccagtcaaac tttacgtgat acatgcaaat
    14701 aatttagaag tagcgattgc ggagaaagag aaattgcaga aaaaatatcc aaatgcagaa
    14761 atcgaaattg gtcatttcgg accagtcatt ggtacacatt taggtgaaaa agccatcggc
    14821 attgccattt cagcgcaata attcctttta ttcaaaaaac ataccaaaaa ta
//
LOCUS       DACEUZ010000067        14567 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.067, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000067 DACEUZ010000000
VERSION     DACEUZ010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14567
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.067"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(172..1590)
                     /gene="nhaC"
                     /locus_tag="IXG44_001673"
     CDS             complement(172..1590)
                     /gene="nhaC"
                     /locus_tag="IXG44_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAQ1658396.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(1993..3216)
                     /locus_tag="IXG44_001674"
     CDS             complement(1993..3216)
                     /locus_tag="IXG44_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HAQ1658397.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(3247..4086)
                     /locus_tag="IXG44_001675"
     CDS             complement(3247..4086)
                     /locus_tag="IXG44_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HAQ1658398.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(4103..4783)
                     /locus_tag="IXG44_001676"
     CDS             complement(4103..4783)
                     /locus_tag="IXG44_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1658399.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(4785..5858)
                     /locus_tag="IXG44_001677"
     CDS             complement(4785..5858)
                     /locus_tag="IXG44_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1658400.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     gene            complement(6505..7176)
                     /locus_tag="IXG44_001678"
     CDS             complement(6505..7176)
                     /locus_tag="IXG44_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ1658401.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(7263..9941)
                     /gene="mgtA"
                     /locus_tag="IXG44_001679"
     CDS             complement(7263..9941)
                     /gene="mgtA"
                     /locus_tag="IXG44_001679"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HAQ1658402.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            10600..11037
                     /gene="ndk"
                     /locus_tag="IXG44_001680"
     CDS             10600..11037
                     /gene="ndk"
                     /locus_tag="IXG44_001680"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HAQ1658403.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(11059..11925)
                     /locus_tag="IXG44_001681"
     CDS             complement(11059..11925)
                     /locus_tag="IXG44_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ1658404.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(12085..14178)
                     /locus_tag="IXG44_001682"
     CDS             complement(12085..14178)
                     /locus_tag="IXG44_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAQ1658405.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
BASE COUNT         4229 a         3109 c         2480 g         4749 t
ORIGIN      
        1 cgagaggttg tgaaaaagtc gcttagtttc gcgtattaag aaaaattttg tgaaatggct
       61 ttccacattt ttgtataaat ttggcttaat accgagaaac tggcttttga ataccgttta
      121 ttcgtaaaag ggattgtgac aaaagtcttg tcacaatccc tttttatgtt tttattgttc
      181 taattgcgct gtcttgctta ctgcttcttg cggtttcaca aatttttttg agattcccca
      241 acctgtgaag gcactgacca tcgaaaacac aggagaaagc aaactgaaaa atacgaatgg
      301 caagtaagtg agtgtaggaa taccaaatgt acttgctacg aaacttccgg caattcccca
      361 aggcactaag tagttgatta ctgttccacc atcttctagc gttctaccta atacagttgg
      421 atcaaatcct gctttttcat aagtttcttt aaatgcatta ccaggcagaa taacagatag
      481 gaattgttca ccaataaaaa tatttactcc gatacttgtc atcaatgttg taaaaatcaa
      541 atttggtgtg ttcgtcatct ttcttgatac tcgttccatc acggttgaga tcaagccaaa
      601 ttcgatcaac aatccaccta atgacaaagt tagaataatc aatgacacag taggcatcat
      661 actttcgatt cctccgcggc taagcagtga atcgatttgt tggtttccag tatttgaaac
      721 aaaccctgat tcgatgaccc cagccaactt cgttaaggaa gtttgtgggt tttgaataaa
      781 aatcattcct actgctacag cgatattcaa caaaatcgtt aaaattgccg gcaccttttt
      841 ccatgcacac agtaacatta aaaataaagg aatcagtgac cagattgaaa tagtaaagtt
      901 tgcttctaga acatcagtca cttcacgaat agatgaaaga cttgcggctt tacttgtgtg
      961 tcctaaaatc atgaataaaa agaaagaaac aacaaatgcc gggacagttg accacatcat
     1021 atgcttgatg tgtttgaaca aatcggcatc aacaatagct gctgctagat ttgttgattc
     1081 ggataatgga gacatcttat ctccaaaaac ggcacctgaa acaatagctc caacaaccag
     1141 cgctggattt aagcctagtg ttgtcccaat accgaaaaat gcaatcccaa tcgttgacat
     1201 aacagtaaag gcactcccta cagcgctacc tacgattgca cacacaacaa atacagaagg
     1261 gacaaaccac tggacactga tcaatttgaa cccaatgacc attagagttg gaatgatccc
     1321 tgcttgtatc catacagcaa tcaatgaacc gactaaaata aaaataaaga ttgggatgat
     1381 tcctggtttg atgccattga tgattccttc gtgcagccaa tctgtcgggt atctttttat
     1441 cactgcatac aacatgacca ttccgatcgc tgctaagatc gtgatattag gtgagacctt
     1501 taatccgaca acgccaatcg caatgcctaa aacaataacc attaatacaa gtgttgcttc
     1561 taaaaaactt ggtgttcctt tttttcccat gataatctct ccttttttag ttgttcttat
     1621 gttctaaaaa agttaagata tcggattact ccgccgcaat tcatccgcga ttcctccttt
     1681 ataaaaataa aaagacctgt tttaaactaa ggacgaatga ctccgcggta ccaccttaat
     1741 taaaaacaga tctgtttttc actttcaaac ttttattcaa atgtatggac tttccatgta
     1801 attcgtcaat taggcttgat cggttcacag cgaccaccga ctttctgaac aaaacaccta
     1861 accgctactg cgtgaaaagt tgacttgtac gaagtttacc ttccataaga tcatttgtca
     1921 atgaaaaaga aaatattttt tcatttttct gaaaattatt tttataactt tcacacacaa
     1981 gtttattttt tactagaaag tagcgccctc ttgttttttt cttgctacta tttcttgatg
     2041 ttcttgcttg attctttcta atagctttgg ctctttcaac agatcaagag ctgtattggc
     2101 gagtagttct gctgcaatcg caatagaagc aaggcctttg tcacttttag cagcagcttt
     2161 gaattcttct gtgtgtccgg caatatatgt ctcagagatt gctaccgtag gctggattgt
     2221 tggtatgact tggctgacat ttcctacatc agaagaaccg agacttgttc tttgttccgt
     2281 ttctatttct tcatctggaa cacctgcttc ttttgcatgc tggaagaata agtcatcaaa
     2341 agaaggagta acgatgacat catcaactgc attttggaac aatccaaact caaaagtcgt
     2401 gcccgtactt atcgcagcac ctcgtacgat attttccact ttttcatata ctttatctaa
     2461 tgtttgccga tttcctgcac gtaaatagaa acgtccagca gcgtaatctg gaatgacgtt
     2521 ggcagctact cctccgtctg taatgatccc atggatattg acatcttttg gcaattgcaa
     2581 gcgtagagcg ttgattccat taaacacttg gatcaatgct tccaaagcat ttactccttt
     2641 ttcaggtgct gctgctgcat gggaagcgac accgtaaaat ttgatgtcga ctggatcatt
     2701 cgctaaagat tctgtcgttt tcccataacg aaaagctgga tggacgcata acgctacatc
     2761 gacatcctca aaaaaacctt ctcgaacaaa gctgccttta gctgatccgt tctcgccccc
     2821 ttcttcccct ggggtgccat atacacggat ttctccacca gtttcatcaa tgaattgctt
     2881 cactatgctg gcggctaata cggagtaagt accaaacaaa ttatgcccgc atgcatgacc
     2941 aatacctggt aaagcatcga attctgccaa aaaggcaatt gtgggaccgg gtttattgga
     3001 aatataacgt gcatcaaagg ctgttcggtg tccagctact tctttttcta cccaaaacct
     3061 ttctttttct aattgtttaa tcaacgctgc tgaggaataa aactcatagt tacttacttc
     3121 aggatgttcg tggatatcta gagcaagtgc ttgatattct ggtaaacgat attggattag
     3181 atccttcaat tggtttgttg agattgttgt tgtcataaaa aattcctcct atgctagtca
     3241 tttttcttat tttctcccga atgtttccca tgcaggcacg cttgatcctt ttgatgtttt
     3301 ctcgattact ttgactgtgt cttctgtttg ataagcgtct actatttttt gatacgtttt
     3361 attgttttca tcttcttttc ttgcaacgat gatattcaca taaggacgtg catggtcatc
     3421 cactggttct aagaagatgg catcagaagc aggattaaat ccagcatcta cagctacacc
     3481 gctattgatc actgaaatgt ctacatctga caaagcacgt gctgtttggg aagcatctaa
     3541 ttcagaaatg tctagatttt ttctattctc ggtaatatca gaaacagtgg gcgcttgtcc
     3601 ttttgcctta tccactttga tcaatccagc agtttgcaat aacagaagag ctctgccgcc
     3661 atttgtaaca tcattaggga ttgctacttt tgctccgtct ttgatatcag ctacatcctt
     3721 caaagtgtta gaataaattc ccagcggtgc attgacagta tttccgatcg atactaaatc
     3781 agttccgtgt tctttattgt aattgtctaa aaagaattga tgttggaaag aattcaaatc
     3841 aatcgatcca tctgctaaag ccacatttgg ttgattgtaa tctgtaaatt tcacgtattc
     3901 caaattaatg ttctcctttt tcaaacggtc tttcacgttg tcccatacat ctgtatcttc
     3961 tccaataatc cctagtttta cgatagtttc ttttttgtca gttgtatctg cttttccttg
     4021 gctacaccct gccaaccaaa taattgctac aaatgtaatg actacaaaac gaataatttt
     4081 tttcattttt tctcctccac tttcaatgtg taattttttt aatcagttga ttactgataa
     4141 attgacttaa aaaaactaag attagaatca ataatgtagc cacaatcgtt acatctgttt
     4201 ggaatcgatt ataccctctc gatatagcaa gattccctaa tccgcctgca cctactgctc
     4261 cagccatggt agtcagtccg atcacgttga tgatagtcaa cgccgagacc cgaatgatac
     4321 caggcaaacc ttctaacaaa tacacacgta aaatgatacc gattggactg gttcccatcg
     4381 cttctgccgc ttcgatcacc ccaggatcaa cttctagcag tgcgttctcg atttgacgag
     4441 caaaaaacgg aacaactcca aaaatcaatg gcactaaagc tgctttcgca ccaatcgtcg
     4501 ttccaactaa gaaacgagta gtcaatgcta aaagagaaag caagataatg aaagggatgg
     4561 aacgaatgat attgatcagc ttatcaagta tttcaaatag cagacggtta gctagtaatc
     4621 cattagggcg tgtcactact aacgtcacac ctaagatgat tccaatcatc ccgccaatga
     4681 atgctgtcca aaaagacata tatagtgttt gcttcgtcgc ttcgacaaat tcatccggaa
     4741 tcaaataaac attgggcaaa tatgttttaa agaaatcagt cattttatct tcctccttcg
     4801 atcactttga tacgactatc taaatcgttt ttttgtaaaa tatttatttt tacaccagat
     4861 ttgatcaaat aatccaatgc ttttttccgc tgtttggttt ctccagctaa agtaacgatc
     4921 aaactcccta acggcgtttc ttgtaataac tctacattgc catataaaat attcgctgtt
     4981 acttgatatt tgctgtacag atgagcgatt agaggttcgt tcgtctgatt tccaatataa
     5041 cttaattcca ccagccattt attggcgatc tgatcggcaa aagcagaatg ggcaatgatc
     5101 gtttctaatg cttggtcaag atgagtagct gttcgaataa aatctttggt caattcttct
     5161 tcaggattgc tgaagatctg gatgctgcta cctttttcaa cgattcttcc atcttccata
     5221 accgctacct tgttgcagat ttctttgaca acttgcatct catgagtgat caggacgatt
     5281 gttaaaccta actggcgatt caattttttt aacaatgcaa ggatctgaag ggtcgttttc
     5341 ggatcaagtg cactggttgc ttcatcacat aataatacct taggttcatt cgccaaagct
     5401 cgtgcgattg ctactcgttg tttttgtccc cccgacagtt gtttaggata agcagatgct
     5461 ttttcttcca aaccgacaag ttccagtagt tcattcactt tttgacgacg ttcctgtttc
     5521 gattttccag aatactttaa agaaaaatca acgttgtcaa aaatagttcg gctttccatt
     5581 agattgaaat gctgaaagat cataccgatg gcctttcttt cttttcgcaa ttctttggga
     5641 gagagctgac tcagattcgt ctgattgaca atgacttctc ctgctgttgg tttctgcagc
     5701 aaattcatca cacgaactaa cgtacttttc cctgcacctg agtaaccgac gattccataa
     5761 acatcgcctt tttcaatcgt taaatctaca gaatctactg cctgtatctc tttttctttt
     5821 tgtttgaaac atacagaaac atctcgcaat tcaatcatta aaattcctcc ttatctaaaa
     5881 aaagcgtcct caagaaaatg aaaatgtcat tttcttgagg acgctttttt gcgtgttacc
     5941 acctcatgtt caagctgtgt tcacacacaa ctcctcttca agtacgatca tactctagca
     6001 agatatcggt cgcagccgtc atcgccttac atctatgctc gggatgaatt gctcgatggc
     6061 cattttccaa tagtcgttcc ttgcttcttc tcagctaccg aagctctctg tcaaaggttc
     6121 ttctatcgta cttaccattt caatgcaaat aatattcaaa aaaaaagtcc ttttactaac
     6181 ataaactatg tcagtaaaag gacgacgtat cgcggtacca ccttttttcg gaagtttcct
     6241 tccctcaagc ccaacatctc aaaaagagag attggcgttc gttttaacgg tcgaacagtt
     6301 ccgaatttgc ctacttatcg acaaattatt ctccaaggcc attttcagct tttcttactt
     6361 gactcttctc accagccaga gcctctctgc aaagtttcca aacttacttt ccttttcaac
     6421 gaaatcgata ttttgttgtt gggattaatc tacatgagga aaactgaaaa gtcaagtacg
     6481 aaattcaaat tattttttct atatttattt ttctgttttt ttatttaaca tttcaataac
     6541 agttcgaaga tcttttcctt ccgcaagaag tttcaatttt tccttttctt cgacggtata
     6601 atttcggaca aaactggaat caggtatcaa aacaacgcct aatcctgctc gtgaagcagc
     6661 tgttgctcct actaaagaat cttccactgc aaaggctgaa ctagcttcta atcctgttcg
     6721 ttccaaagct gtcagataag gaatcggtga aggcttgttc tccgacactt cgtctccgaa
     6781 tgtaaacgtg tccaagtaat cagctaattt aaagtgatcc aagatatctg ttgctctttt
     6841 cgtaacagtg gtcgtagcaa tgcctagttt catatgacgt tcttttaagt ctgccaatat
     6901 ttcttttgca tatggtttta agtggatggc tccgcttgtc agctgttgat aaaaatattc
     6961 ttcacgcttc gctcggactt cctgtacttt ttcagggcta cctgctgctt tcactaaaat
     7021 atcaaaactt tgtttccagt tttttccgcc ccaagcgtca acgatttctt ttgggatatg
     7081 gatatcaaat tgctgaaatc cccaaagcca gccatcacga tacgtttttt ctgtgtcaac
     7141 taacaaacca tctaaatcaa atattgcacc tttcatctat acattaatcc tttccaaaac
     7201 aatacacata gtttagcacg actggtccaa aaagaacaga agcaaagaac cagccttctc
     7261 gtttataaga gttcttggaa tcgacggata tagattttct tcacagctgt cacaagcgcc
     7321 agataagcga tgactgtagc aaataaccat aaccagtaat tgccaggaag tggcatcaag
     7381 cctaattcag caccaaaact tgtaaaagga aggacagaac cgacaataat tcctaatgta
     7441 gtgataacaa acatggcaaa tgttgcgttg ctttggataa atggtacttt cggtgtacgc
     7501 agtgcatgga gcactaaggt ttgtgtccat agtgattcga caaaccatcc tgcatggaat
     7561 aatgcgatga aaatcactcg ctgtgaacta tccaatgtat ggaaacttcc gcccactaca
     7621 gcaggacaaa taacaaaata catcaacaag taagtagtaa tatcaaatat cgaacttgtt
     7681 ggaccaaagt aaaccatgaa ttccccaata ctagatgcat tccatttttt tggctcgtcc
     7741 aaatattctt tatccatccg atcccatggc aaggaaatgc aggaaacatc atagatcaga
     7801 ttcaaaaaca aaagttgtaa aggcagcatt ggtaaaaatg gcaggaacgt acttgcaatc
     7861 agcacggaaa acatattgcc aaaattagaa cttgctgtag ccttgatata tttcatgata
     7921 ttcccaaaag tttctcgtcc agaaagaagg cctctttcca aaatcgtcaa atctttttct
     7981 aaaaggatca cgtctgcaga ttctttcgca atatccacag ctgtatctac agaaataccg
     8041 atatcagcca ctttcattgc aggagcgtca ttgataccat ctcctaaaaa tccgacagta
     8101 tgaccatttt tacgtaaagc tgtcgtcaaa cgtgcttttt gctgaggatt caattttacg
     8161 aatacttcat ggtcttcagc tacttgtgct aattcatgat cactcatttc cgagattttc
     8221 tcaccggtaa ccaattcatt gatctctaga cctacttgac ggcacactga acgtgtaact
     8281 aattcgttgt ccccagtcag aacttttaca gctacaccat gttctttcag agctttcaac
     8341 gcttgtttcg ttgtttcttt tggtggatca aggaaagcaa gataaccaat caaaaccatg
     8401 tctgactcat ctttgactga aaattctcca acgacgcttg gattggtctt ttgtgcgaca
     8461 ccaatcactc gtaagccatc ttcattaagt gactgtactt ttgcaagtac ggcttctttt
     8521 cgttcttttg ttaatggtgt aacagtaccg ccaagatcaa taaatttaga aatgttcagc
     8581 atttcttcaa tcgcgccttt tgtgatcatt tgtgttttac cagctggatc agcaaccaca
     8641 acactcatac gacgacgttc aaaatcaaaa ggaatttcat ccactttttg ataattgatc
     8701 ttatctgtat ctaaagtttc tgttgcttca tcaatgatag ccacatctaa caaatttttc
     8761 agccctgttt gatagtagct atttagatac gcatggcgca aaacacgatc atcttcattt
     8821 ccttcacaat ccatatgata ttcaagaatg attttatctt gtgtcaatgt ccccgtttta
     8881 tccgtacata acacatcgat tgcaccaaag ttctggatgg cattcaaatt tttgatcact
     8941 gttccttttt tggccatcac acttgctcct ttgaccaaat tcgtcgtaac gatcattgga
     9001 agcatttcag gtgttagacc aactgctaca gatagtccaa aaagaaaagc ttctagccaa
     9061 tccccttttg taagcccatt gatgacgata actgctggag ccatcaatga cataaatttg
     9121 atcagtagca tggatgtttt atggatgcct aattcaaagc ttgattgaat tggtttttca
     9181 gatagcgttt ctgctacatg accaaatagt gtttcatttc caacagaaac aatgattcct
     9241 tccgcacttc cactgatgac attacttccc ataaagacaa gattttcgta acttgtttcg
     9301 ctttgctgat cgtcttcaca tactttcgca tgcttttcta ctggatagct ttcccctgtc
     9361 aatgctgctt gggaaatgaa taaatccttt gcggacaata aacgcatatc cgctggtatc
     9421 atatcccctg ctgaaagccg aacaagatct ccacagacga tttcttccat aggaatctct
     9481 gtgtactttc cattacgttt tacagctgcc gttactttca caagtgattt cagtttttca
     9541 gccgcttgat tggaacgtac ggactgaatc aacgtcattg tcccgctgac gataaccata
     9601 gtgaagataa tggctacacc gaaaagatct ttatctccag gagctgctaa tacatattcc
     9661 gtgatgaatg aaataattcc taatgcgatc aacactaatg taaatggcgt gatgtatgct
     9721 ttcaatattt ctacaatgaa tggtgttttt ttgccatatg aaatcgcatt ttcaccgtaa
     9781 ttttctcttg aacgttcgat ttgttcttct gtcaatccat ttgctgatgc atggaatagt
     9841 tcaaataatt cttgtttcgt ttttccagca aaagctgcat agattttttg tgtatctttc
     9901 tttttttcca tctcaatctc tcctttgtgt ccaaaagaca gctgttctcg ctgcttttgg
     9961 tctttagcta ttcatcggat cgatagagaa aaaactgaaa ggggctgaga acagatagat
    10021 aatagccctg gcctttcctc tagatccttg tttttcgaat gactgtcatc catttgttct
    10081 catctccttt actaagtaat tttaattcat gacttccaat catttttcga atagttattt
    10141 ttccaattag accacctcct ttttattttt caccgatggg gatttgcggc aacaaaaaaa
    10201 cctctcgcca aatactcagc gaaaggtttg aaaaagcata ttaaaaaaag cttctcagat
    10261 ctcatcgttg agttttgaca ctgtacaacg taaaaagcag atacctgctt ttagctacct
    10321 tatgaaaagc cttatctcga caattcctgt tctacccatt ggcgtctctc gacgtttttg
    10381 ggcagtagcc tatgtttgta taggagcctc acctaacaaa tcttattcgg ttttccttcg
    10441 ttaatataac aactaaaaaa gaggatgtca aatcaatttt ccatgttttt catacttttt
    10501 ttaaaataca cacgttatat tcctgtctca cgcggtttta tttgtctcat tttccacctc
    10561 ccgctataat agaaatatag taggaaacga ggtgctgtta tggaaagaac acttgttatt
    10621 atcaagccag acggcgtgag acgccattta gtcggaagca tcatccaacg ttttgaagca
    10681 aagggactgg ccattgctga aatgaagttt gaaactatga ctcctgaatt agcaaaagaa
    10741 cattatcagc acttgactga gcgcagtttt tttgatgaac tgatcgatta catgacttcc
    10801 ggtcctgtcg tttatcttgt gctggtcggc gaggaagtga tcgatatcgt ccgtaagatg
    10861 gttggagcaa caaaagctgc cgatgctgtt ccaggtacga tccgaggaga ctacgcgctg
    10921 ccggggactg aaaacatcat tcacgcttct gattcccgtg atgctgcggt aaaagagatc
    10981 gcccgctttt tcccgccatt cgatataaac aaaccgtctg aaaaagcggc gaagtgatac
    11041 aaattctaaa ctgctggtct agttgctggg ttcagcaatg gccagcagtt ttttctctaa
    11101 ggacctccgt tgattctttc ctaacatata ttcgagcact gtgaagaatt cttggtagtc
    11161 tttcgtcttt tcttccccta aagcagcaga aatcaatctt tgataataat ctgccatcaa
    11221 tgtttcatag atcactgtac ttctttcttt atgtgctaag ctccgtttca tatcttcatt
    11281 cacactacgt gcctttttca accgaccatt ttctatataa aacgaagtca ggttcattaa
    11341 aaaagtaaag agatactgct catgattgtt caactgtgct aatcgtcgca ttcttttttt
    11401 gattttcaaa taattgttct caatgaaccg atcactgaat atccaaggaa aatcagctag
    11461 aatagatagt tcaaattgac catagtctgg tatctgcatc aagtattctt caatgattcg
    11521 gcattcataa cgtaaaaatt caaattctgt ctcagatact tctgtttttt cgaccatgtg
    11581 aagattcaaa accaaataat acagatcttc tgttttctta aaatgcctac ggttattgct
    11641 gatctcttta taaggatcaa agctgtcttc ttgtacccga tctagcattc gtcttttttt
    11701 ctttaacttt attggttcat ccagctggtt tcgcatccat tcatattctg caagtgtcat
    11761 gttcattcgc tctaaaaatg atctcaatgt tacaaatgac atttttttca ctgaatttgt
    11821 ctcaatcccg actagagaag tcttaaaaca gattccttcc gctaattggg actgtgtcaa
    11881 cttcttgttg actcgaagtt tcttgatcaa ttcaccatca tacataatag ttctcctttt
    11941 taatttaaat ttttccacat tcaataaata gatacgttaa aaaggaaaaa tacacgaaaa
    12001 aaataaaaaa atgttttttt cataaaaaat accaaccttc aagcattaga atgttgtcta
    12061 actactgaag gttggtttga ttgtttattg attatttttt tgctgttcaa cgtctgttct
    12121 tgttactgga tataagttgg ttgttgtttt attaccattt tttgaaaaga cacttgtcga
    12181 atgttcgcct ttttgctttt cgattgcttc attacgttcc gcttcatttt tatagtcata
    12241 ttttttcgga tcaatcgtct caaatccgtt tggcacataa aaccgcaaga gattttcctg
    12301 attcagctta tctgaaagac ttaacgcagt cttgactttt tcctgatctt tggagatttc
    12361 ctcatcgatt cctgctgctt tcgtatcgat gatttctcct gtctgattat tgtaagcttt
    12421 tcctcctagg acggtgtatt tgggtgtcac aaagttccca tttcgaaaag caactacttg
    12481 atcatgctga gaagataata agtctgtacc aaaatgaaga taatctttat catcgattcc
    12541 caatagatgt agtaatgtcg gcaaaacatc gatttctcca ccgtattcat gattgactgt
    12601 ccctttagta taaccaggca tatggatcat caatggcact cgttgcatct gtgcgttgtc
    12661 aaattcatcc catgtatctg gatctttacc aagagcgctt gctaaatctt tattatctgt
    12721 attcgagatc ccaaaatgat ccccgtaaat cacaaagatt gaattttggt aaataccaga
    12781 ttttttcaaa tacgtaaaaa attgttccaa tgattgatcc agataatgtg ccgttcgaac
    12841 ataattatct actgtgctat tgccagtatt caaagatgga aagtcgaaat tcttgtcatc
    12901 cgtataataa ggcgtgtggt tggttacgga aagaaatttc gtgtaaaacg gctgttgtag
    12961 atgctcaaga tattgggcag attctctgaa taaatcttta tctaatatcc cataacctaa
    13021 cgtttcgtcg gattcatcaa aataagatcg atcaaagaaa ttgtcataac ctaagttttt
    13081 atatacatga tctctattcc aaaaactagc tacattccca tggaaaacag cgcttgtata
    13141 gccttgcttt tgtccaagga tcgctggtgc ggcttgaaag acattgtctg acccaagttc
    13201 tgtgaataat gagccttgag gtaaaccaaa tgtgccagtt tccaacatat tttcagcatc
    13261 acttgtcttt ccctgtccta cttcatgaaa gaaattgtca aagctgatcg ttgcttgatt
    13321 ctggaaaata ctatttaaaa acggtgtgac ttcttgtccg tctaccttca tattaatcaa
    13381 aaactgctgg aaactttcta aatggagaac gatcacattt ttccctttag cgatcccaaa
    13441 tgtttcggga tttggctctg cataatgttt ctttgtataa tctaaaactt catcaatccc
    13501 attactgctt gcatgcgcgc gaacactgct agtcattcct gttttgattc cgtcataaat
    13561 agtatacgca tcgattccta gatatttgac aatatatgaa cggtcaaatg ttcgctgcag
    13621 caattgtgga cggttggctt cgctcagcgc tagatttcct gaaaaaataa gccctgaaag
    13681 acaggtaaca gcgatagcca tcggtttgcc gacagggttg cttttgatag gtattttctt
    13741 gaccttcaac caaaccagta gcccaatgaa cacagcgata tccagccagt agatgacatc
    13801 atggggcttc atcaaagaaa aggagcttcc tgacaatcct tgcgacactt tcgaaaagcc
    13861 taatacagat ttgactgtaa tgaaatctgt gaattcccga taaaaaataa tattcaagta
    13921 caacaaaagc gtatttaaaa tatcaatgat caaaagaata gctaacgctg gctttggctt
    13981 tttaatatac aaagaaaggc caaaaaacaa taaggtagtc gcaattgggt tgatccacaa
    14041 gataaagtac tggatcgtac cttccacacc taatgaaaaa tctgcatagt aggcaatgat
    14101 cgtcttcagc cagaaaaaga aaacagctgt caaaacgatt gctaaacgat tttggcacaa
    14161 ttttttaagt tgtgtcacgt atatcacttc tctctattca cattttttct tattttgctc
    14221 ctgaaaaatg aaaatttcct aaaaaagttg ttatcttaag atttttttat tctttctaaa
    14281 gattattccg tccacttttc acagcataaa accgctgcga tgtaagcgat taaactaatg
    14341 aagagataaa agtcttgtgg tgaccgcttc actccacggt attcaaaagc cagtttctcg
    14401 gtattcgtac aaaaaaagag gctgtgacaa aattcttgtc gcagctcctt atttcttgat
    14461 gcaggacagc tttttcacaa cttcttcctt aaacggtgtt ctcctgccaa ctcctcggcc
    14521 acaagtcaca atatttataa aacatgagaa gctgtttttt gaaattg
//
LOCUS       DACEUZ010000068        14292 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.068, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000068 DACEUZ010000000
VERSION     DACEUZ010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14292
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.068"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            132..2603
                     /locus_tag="IXG44_001683"
     CDS             132..2603
                     /locus_tag="IXG44_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ1658406.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(2706..4442)
                     /locus_tag="IXG44_001684"
     CDS             complement(2706..4442)
                     /locus_tag="IXG44_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HAQ1658407.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSVFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(4439..5143)
                     /locus_tag="IXG44_001685"
     CDS             complement(4439..5143)
                     /locus_tag="IXG44_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1658408.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            5274..5777
                     /locus_tag="IXG44_001686"
     CDS             5274..5777
                     /locus_tag="IXG44_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HAQ1658409.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            5737..6075
                     /locus_tag="IXG44_001687"
     CDS             5737..6075
                     /locus_tag="IXG44_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HAQ1658410.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            6096..7514
                     /gene="ffh"
                     /locus_tag="IXG44_001688"
     CDS             6096..7514
                     /gene="ffh"
                     /locus_tag="IXG44_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HAQ1658411.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            7626..8204
                     /locus_tag="IXG44_001689"
     CDS             7626..8204
                     /locus_tag="IXG44_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HAQ1658412.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            8208..8729
                     /locus_tag="IXG44_001690"
     CDS             8208..8729
                     /locus_tag="IXG44_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ1658413.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(8808..9362)
                     /locus_tag="IXG44_001691"
     CDS             complement(8808..9362)
                     /locus_tag="IXG44_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAQ1658414.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            9615..9890
                     /gene="rpsP"
                     /locus_tag="IXG44_001692"
     CDS             9615..9890
                     /gene="rpsP"
                     /locus_tag="IXG44_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HAQ1658415.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            9902..10153
                     /locus_tag="IXG44_001693"
     CDS             9902..10153
                     /locus_tag="IXG44_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAQ1658416.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            10278..11069
                     /locus_tag="IXG44_001694"
     CDS             10278..11069
                     /locus_tag="IXG44_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1658417.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            11239..11760
                     /gene="rimM"
                     /locus_tag="IXG44_001695"
     CDS             11239..11760
                     /gene="rimM"
                     /locus_tag="IXG44_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HAQ1658418.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            11750..12511
                     /gene="trmD"
                     /locus_tag="IXG44_001696"
     CDS             11750..12511
                     /gene="trmD"
                     /locus_tag="IXG44_001696"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HAQ1658419.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            12647..12994
                     /gene="rplS"
                     /locus_tag="IXG44_001697"
     CDS             12647..12994
                     /gene="rplS"
                     /locus_tag="IXG44_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HAQ1658420.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            13252..13545
                     /locus_tag="IXG44_001698"
     CDS             13252..13545
                     /locus_tag="IXG44_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HAQ1658421.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
BASE COUNT         4794 a         2567 c         2905 g         4026 t
ORIGIN      
        1 ttttttttgc tatatgacag gaatgaaagg tgtttgcaag cttaatgtga ggaacacttt
       61 ccatatctgt tctatactgg ataggagaag tttggaaata tccagaaaac gaaatcaatc
      121 gaggtagcca catgaagaat aaatggataa atcgaagtaa aaaaaagaaa gtaacacaaa
      181 gatcatcaga taaagtcatc cgtctttttc catcgtataa agaaggatta acggcagatc
      241 aagtcaagga acgaatagaa aaaggtgcag caaataattc agtcgatccc acattcaaaa
      301 caaatcagca aatcgtattg gaaaacatat ttacgtattt caatttgatc tttcttatct
      361 tagccatttt gctttgttta gtagaatcct ataagaactt gactttttta ccagttatca
      421 tagccaatac aggaatcgcc atttttcagg aaattcgatc caaaaagatc ttggacgaac
      481 taaatgtttt aaatacagca aaagtcaatg tcatacgaga cgggatagaa caaacaatag
      541 atatagaaga ccttgtaata gatgacattg ttattttaga gactggatat caaattccag
      601 cagatgcaga ggtcgtggaa gggaaactgc aagttaatga ggctttattg acaggcgaag
      661 cagatgaaat aaccaaagaa ataggtgatc cattaatgtc cggcagtttc attgtttccg
      721 gaaaagctta cgcccgatta gacaaagttg gagcagattc gtatatttcc cagctgacag
      781 tcaaagccaa gacaatggga gaaggagaac agtcggagat gattgcttct cttaatcaat
      841 tgatcaaatg gattgggatc atcattattc ctatcggtat cctgcttttt tctcaaagtt
      901 acttttttaa tggaaacacg atcaaagaaa gtgtcgttgc aatggaagca gcattgatcg
      961 gtatgatacc tgaaggactg tacttgctga cgacgattgc attagcatta agtgcgacta
     1021 aactagcaaa gcaaagagtt cttttacaca atatgaaaag cattgagaca ttggcaaggg
     1081 taaatgtcct ttgtgtagat aaaacaggaa cgatcacaga aaataaaatg tctgttcaaa
     1141 aaatgatcgt ctctaaaaaa caagaaataa tcagtcagtc agatcaattg gaagcatgca
     1201 ttgctgacta tgcaaaagcg atggcaggag acaatgcaac aatggaagct gttaaagcat
     1261 attttacaaa aaccactgat caatcctatc accatgttat tcctttttct tctgtccaaa
     1321 aattcagcag tgtagcgttg gcagacaagg tgtatgtttt aggagcgccc gaaatggttt
     1381 tgcgcgatca attgccggaa tatgcggaag agtttgtttc tttcgctgaa aaaggctatc
     1441 gcgtgttggt ctttggtgtg tacgatggga ttcttgaaga accagtattg acagaagcag
     1501 tcacaccatt aggctatatt ctgattgcta atcctatcag aaaagaagca agagccacat
     1561 ttgattactt caaaaaacag caagtagcca tcaaagtgat ttctggagat aatccgctca
     1621 ccgtatccaa tgtagctgtc caagcaggga tttcacatgc tgatcagtac gttgatgcat
     1681 ctaaattatc agaagaagaa tatgaagcag ctgtcgaaaa atatacagtt tttggacgcg
     1741 tcaagccaga acaaaagaaa atcttcgttc aattgataaa gcgtaacaat acagtagcta
     1801 tgacaggtga tggcgtgaat gatattctag ccatgaaaga agcagactgc agtatagcga
     1861 tggcttccgg aaatgaagcc gccatgcagg cctcacaagt ggtcttactt gattctgact
     1921 tttcacgcat gccagaagtt gttgcagaag gacggcgcgt tgtgaacaat attgagcgat
     1981 cagccagctt gtttttagtg aagaatattt tctccttttt gctctcactg ttttcagtga
     2041 tctttgctct gacctatcct ttagaaccat cacaaatcac attgattagt ttgtttacta
     2101 tcggacttcc atctttcttg cttgctctag aagaaaacaa aaagcgaatc agagggaaat
     2161 tcatcatgaa tgtgatggaa aaagctgtgc caggtggact aacagatatg atcgtagtcg
     2221 gtgcgttggt gatctgcggg gtgactttga atctaaataa aacagatgtt tctacagcat
     2281 ctaccatgct tttgattgca gtagggttcc tcgttctcta taaaatctgt tcaccgctga
     2341 ataaattcag aagtcagatc attcttttct gtgctagcgg taactttttt tcggtcatct
     2401 ttttgcataa gctgttttca atcaccggta tctcagctgt atcacttttg ctgttgagca
     2461 ttctattttt ctcggcagat tcgattttta ggcatttgac cacattggta gaatatttgt
     2521 ttgaacgtaa aaatgaaaaa gagcaaaagc cgtttagtcg cgtagctaaa aaatttctat
     2581 cgatatttaa acgagaaaat tgacgaaaag tatctcaaaa aagagtaaaa aagaacaacc
     2641 actcgtgtat ctttcgaagc aggatgagtc atccaacttt aaaacgcacg agcagttgct
     2701 cttttttaca ttttcggaat ggtaacggtg aaagtcgaac ctgttccaag atgactgtct
     2761 acagtaatcg taccattaag gagttgcacg tagtctttta caatcgctaa ccctaatccg
     2821 gtcccgcctg aatgtcggct tcgcgccttg tctacgcggt aaaaacgctc aaagatccgt
     2881 tcctgatcct caatgtcgat acctatacca aagtcttgga cagaaaacga tagatcatct
     2941 gttgcctgat agcggataat aatctcgctg tcgctttttg aatattggac agcattttca
     3001 atcagatttt tgataattgg ataaaggata tctgttttag tagtaaatgg ttcgttcttt
     3061 ccaatcaatc gaatcgtcaa ccgtttcttt tcgatgattg gctgataatt ttgaacgatt
     3121 tgctggaaat aaggttctgt ataaattgtc tggatctcgt aagaattctc gccatctttt
     3181 gacagctgga tgatttcccg aatcagttta tctaaccgga tcgcatcttt ttgcatgatc
     3241 tcaagaaaag aagtcagagt ttgcgggtca tcttttgcac catccagtaa agtctcggta
     3301 aaaccaatca gtgaagtcac aggagttttt aattcgtgag agacgttgcc aacgaaatcc
     3361 ttttgtagct tttctaaacg tcgcactttc gttaagtcat acgccacccc tagaatctgt
     3421 ccggtacctt ctgaattagt aaagtagcgc aaactcaagt ctaatgtctg ccgaccttct
     3481 gtaagggtga tctcctgatg gatcagcgga tgctcaggag tcacttgatg aatcagctga
     3541 atgagttttg gctcttgaat gacttctgtg taccgctgtt caggttgata gcttaccacg
     3601 tttaaatgta gctgcatctt tggattaagc agtagtaaac gagaatcagc atcatcaatc
     3661 aggaatacgc caatcatcag ttcgttcagt aacgtatata attgctcttc tgtggtagta
     3721 cacgcccgat acatcttact catttgttcg ctcaacgcat taatcgtctg atacaattct
     3781 tcccattgat cgggtgtttg catgattact tccgcctgat taggattttg caccattttt
     3841 tttagtacgg gcagcacggt tttcaatggc tggttttttt gatagatcag ataatagatc
     3901 attcctgcaa gaacaaggaa gaaagacaga aagaagaaaa agacccaacg gcgaaaactc
     3961 tcagttctcg gtaaaaaaac gcttgtcggt tctgcgatac gtaaaatggc ttctaactgg
     4021 ccactttttt tgactggtaa tgcgacatat aaaagatcct cattcaaagt agtacttttg
     4081 cgcaaagcgc tacctaggtt cccaccattc aatacggctt tgatttcggg acgggagttt
     4141 cgctgttcat tcaatgtttc attgtttgtg tcaaataaga tctttccagt agcatccatt
     4201 aaagtaatcc gttcgttcga ctgatggacg aattcttcca aagcagtttt gttttgcgct
     4261 gcttggaggt tttcgacatc taattgccgg atcagcagtt gtgctttttt tgtcaggtag
     4321 ctttcttgct gttccacgat ctgctgttga aaataatggg aaatcaattg ccagccacca
     4381 aaaaacaaac taactaaaag aataagaaag aagagacgtt cataaatttt tttccatttc
     4441 atcgtttggg ttcctgaaac ttatagccaa atcctctaat agtcaccaag tactttgggt
     4501 gttttggatc tcgctcgatt ttttctcgca aatgactaac gtgtacatct acgattcggc
     4561 tttgtcctgc gaagtcaaaa ttccaaatgc ggtctagcaa tgtgtctcgg tcgatcacac
     4621 ggtcttttcg tttcatgaaa taaacaagta attcaaattc tttcggtgtt agttcaatca
     4681 gatggtcgtc tacggtcact tgataattgg tcaaatcagc agtgatttgt ccaatcgata
     4741 gatattcagg ctcagcttca tcttttgatt gcttacgagg ttcaattcgt ctgaatattg
     4801 ctttcatacg ggctaatact tcgcgaggac taaatggttt ggtcaaatag tcgtcagcac
     4861 ctatttctag gccaatgatc cgatccactt ggtcatcttt agccgtcagc atcaaaatcg
     4921 gtgtttcatt tttttcttga cgcagccgct gagtgatctc catgccatcc atcgttggaa
     4981 gcatcacatc taaaatgatg aaatcaaatg attgttctaa tgctaattct aatccttcgc
     5041 ctccatctgt ggcactggtt accttgtagc cttctttttc caaattgaac gtcaagagcg
     5101 ttacaataga aggttcatca tcaacaacta gtattttttt cattcaaatc tcctttaaat
     5161 aggtttcatt ctgtttatat tttagcatag aatcagtaca aagggctatt ttacaaaaga
     5221 aataaatggt ataataggaa ggcactttgc tgaatagaaa ggaagagcag tcaatgattg
     5281 gaatcagtgc atgtttaggc ggtctggcct gtcgctatga tggacagaaa aaagaagtac
     5341 cagaattgat ggaactagtt gaaactggag aagctgtcat ggtctgtcca gaagtgatcg
     5401 gcggattacc aattcctaga gatcccgctg aaatcattgg tggaaatgga ttcgatgtgt
     5461 ggaaagatca agcaaaagta tggacgacaa gcggcgaaga tgtaacggaa gcgtacaaaa
     5521 atggcgcgat caaagcctat caaaaattgc aggaaaaaca cattgatcta gtcatcatga
     5581 aggaaaaaag cccttcatgc ggaacccatt cgatttatga cggaagtttt tccggaataa
     5641 aaaaaaatgg tgccggagtg gctactgctt attttattca gcaaaaaatg actgtgctct
     5701 ctgatgaaga atggctgaaa actagaggtg agcagaatgg aaatcgaaaa gaccaatcga
     5761 atgaacgcgc tctttgaatt ttattcgacc ttattgacag aaaaacaaat gaactacatg
     5821 gaattatatt atgcagatga tttttcatta ggtgagatcg cggaagaata cgaagtaagc
     5881 cgacaagcag tatatgacaa tatcaagcgg acaagcaaga tcttggagga ttatgaaaaa
     5941 aaactccatc ttttttctaa ttatattgtt cgagaacagg tattggaaaa tatgactgca
     6001 tatattacag ataagtaccc ggaagataaa aaactacttg aatatgtaca gcaaattcaa
     6061 gcaatcgaag aataaataaa aggaatgaag aaaatatggc atttgaaagc ttaactgaac
     6121 gtctccagca ggcgatggga aaaataagaa aaaaaggaaa agtatcagaa gctgacgtaa
     6181 aagaaatgat gcgggaaatc cgccttgcct tactagaagc cgatgttaac ttgcaagtag
     6241 tcaaagattt tactaagcgc gtacgcgaac gtgcggtagg ggcagaagtg ctggaaagtt
     6301 tatcacctgc acaacaaatc gtaaaaatcg ttgatgaaga attgacgatc acacttggtt
     6361 ctgaaacagc agagttaaat aaatcaccaa aaataccaac tgttatcatg atggctggtc
     6421 ttcaaggggc aggtaaaacg acctttactg gtaaattagc caattattta aagaaaaatg
     6481 aaaatgctcg tccgctccta atcgcagggg acgtatatcg accagctgcg atcgaccaat
     6541 tgaaagtatt aggtcaacag ctagacgttc ctgtatttga tatgggaact gaagtcagcc
     6601 cggtagagat cgtccgtcaa gggatggagt tagcaaaaga aaagaaaaac gactatgtat
     6661 tgatcgatac ggcaggacgt ctgcatatcg atgaaacatt gatggatgag ttgaaacaaa
     6721 tcaaagaatt gacgcaacca aatgagattt tgcttgttgt cgatgccatg actggtcaag
     6781 atgccgtcaa cgtagctgat agcttcaacc agcaattagg tattacaggg gtcgtcatca
     6841 ctaaacttga tggggatact cgaggcgggg cagcactatc gatccgctca gtgacaggcg
     6901 caccaatcaa attcatcggt tctggtgaaa aattgactga cttagaagta ttccacccag
     6961 atcgtatggc tagccgtatc cttggtatgg gggatatgct gacattgatt gaaaaagcgc
     7021 agcaagacta cgacgagaaa aaagcggaag aacttgcaca gaaaatgcgg gaaaattcat
     7081 tcgatttcaa tgactttatt gaacagcttg atcaagtgat gggtatgggc ccactggaag
     7141 atctaatcaa aatgatccca ggaatgaatc aagtccctgg aatcgaaaac gtcaaggtag
     7201 atccaaaaga tgttgaacgc aaaaaagcga tggtttattc tatgacgcca gcagaaagag
     7261 caaatcctga tctgttgaac ccaagccgtc gtcgccgaat tgctgccggt tctggaaaca
     7321 gtgttgttga agtgaatcga atgatcaaac aattcaagga atcaagaaaa atgatgcagc
     7381 aaatgtccaa aggggatatg aacatcccag gtatggacca aatgtttggt tcaggcgtca
     7441 aaggaaaact aggcaaaatg gcgatgaatc gaatgatcaa gaaaaataag aagaaaaaga
     7501 aaaaacggaa ataagatgaa aaaaacagct tcggtacaga ctgaaaatgt ctactgccgg
     7561 agcttttcta tcagcaattt ttattcttta ctaatacaat tactctaaac gaagaggtgg
     7621 cacagatgag atgtccgtgg agtgaacaaa cagaaagtat gcggcaatat catgatactg
     7681 tctggggtgt tcctgaatat gatgaccaaa agctgttccg aaaattgatg cttgatatta
     7741 atcaagccgg attgagctgg cagacgattt taaataaaag tgaagcattc gatgaagcat
     7801 atgatcgatt cgatatcaaa aaagtcgcaa attatggaga aacaaaaatc agtgaactaa
     7861 tggaaaacaa aggaattatt cgtaaccgaa gaaaaatcga agcagccgtc caaaacgcca
     7921 aagcagtctt gaaaattcaa gaagaaatcg gcagtttttc agagtatctt tggtcgtttt
     7981 ctaatggaaa aatcgttgat tcttcttatc attcacaaga agaagtccca acaacaagtc
     8041 ctctttcaga tgaattagca aaagatctaa agaaaagagg atttaaattt atcggaagca
     8101 caacaatcta tgctttttta gaagctgtcg ggatcatcaa tgatcacctt gacagttgtt
     8161 tccgcaaaga agaggtcgta cgtatcggca gaaagggcga ataaagcatg cgaaaaatca
     8221 tattttatgg tgctataagt ttagatggct atttggcaac gaaaacggat gatcttcaat
     8281 ggctttttga cactccaaca ggtaagcaaa ctacttatga agctttttat gaaacaatcg
     8341 atacgaccat catgggcaga aaaacttatc aggaagcaaa aaaatatttg gagaccgaag
     8401 caatttatcc agagaaaaag aatttcgttt tttccaccca cgcacaatta caattgccag
     8461 atgctgaggt cgtaaacgaa gatcctgttg cttttctaaa aaatctacaa atgacagaag
     8521 gaaaagatat ctgggttgtg ggtggcggtc gattgatcaa accgctattg gaacatcaaa
     8581 tgattgatga atggtatatc cagattaccc ctgtattatt aggggacggg attcctttat
     8641 tccaaaaagg aacatatgaa gcgcgttttg aaatgctaga cacgacacga ttcggtgaat
     8701 ttatcgaatt gcattatgta cgaaagtaga agtaaataaa ataaaaaagg caactatatg
     8761 cagaaatgtc ctgtacatag ttgccttttt tattaccgtt atgcttttta ctctgttaat
     8821 ttgttagaaa aatctagata cgcatctaaa atcgtttgga agaaacttaa tgttccttca
     8881 acgatattac cattttcatc aattagattc gcaacgtttc cgatatatgc ttccggttgc
     8941 tgcaaggttg gcacattcaa aaagacaagt gattggcgta gatggtggtt tgcgccaaag
     9001 ccgccgatcc cacctggtga tgcagtaacg actaaacctg gtttatgatc ccaaacactg
     9061 tgtccataag gtcttgagcc tacatctaaa gcatttttca atactgctgg cacagaacgg
     9121 ttgtattcag gagtaacgaa aacgacaccg tctagtcccc tcacttcctc acgaaaacga
     9181 gtccattcag caggagcctg ttctggagtt tcgagatcct cattgtaaaa tggcagatca
     9241 tcgattttta caaatacact ttcgtaccct tcaggaagca atttcgcaaa agcttctgct
     9301 acttttttgt tgtaagaatc ttttcttaaa ctaccaataa aaaatccaat tttttttgtc
     9361 atacgatttt tcctccttca aactatctat aataatctta caaaagataa gtaactggag
     9421 caaataaaaa cacttactga caaaagctac atagaagata gattacttat ttttaacaaa
     9481 aaagaagtct aaaatcttct attttctaaa aaaaatcgaa tttaatcaag ctgtaaagaa
     9541 aaaaagcttt acagggtaaa aaaaaactgt tacactacat cttgtgaata aaacaatgga
     9601 ggtggaaata aataatggca gttaaaatcc gtttaaaacg tatgggttct aaaaagagtc
     9661 cgttttaccg tatcgtcgta gctgattcac gttctcctcg tgatggacgt ttcatcgaaa
     9721 ctgtaggtac ttacaatcct ttgaaagacc ctgcagaagt agttttaaaa gaagacttag
     9781 ttcttgactg gttatcaaaa ggtgcacaac cttctgacac tgtacgtaac atcctttcaa
     9841 aagaaggcgt tatgcaaaaa caccacgaag ctaaattctc aaagaaataa ggtgaccgga
     9901 tatgaaagat ttaagcgact tagtcttaac aatcgttcgt ccgctagtca cttatcctga
     9961 tcaagtcaag ttagacgtcg tagagtcgaa agacttttac gagtacaatc taacagtggc
    10021 tcctgaagat attggccgta tcattggaaa acaagggcgt gttgctaaag caatccgcac
    10081 aatcgtttac ggtgttcgga tcaatgcacc gaaaaaagtg cgtttgaata tcatcgatga
    10141 taaggaaaag taaataaacg tgctgtactc gaattttcga gtgcagcttt tttgtttttt
    10201 ttattaattg aagaaatgat tatttggcaa aacaagcaag aaagcgtatc cttataggag
    10261 gagggagtga acagccaatg agaaagaaaa tgttatttat tccatttgct ctattgttaa
    10321 gcgcttgtag ttcggttact caagaatcag acaatacaca tctatcaagc agtatatctg
    10381 aaactgttac cagtccagaa aaaagtagta ccaaaacggc ttccaccact gaacaaacaa
    10441 tggctacctc aaataatgag aaaaagactg ctttagacca gttgaaagaa caacagccaa
    10501 atgttcccat gcctctagat gtaccagtct cttcaggcta tctcaatatc gcagcgacac
    10561 atacaaagca aggttattct attttatatt acagaacaga tcgtcccctc gggttgaatg
    10621 ctgacgaatt gaatcaagaa acgcctattg caacctatct atatcaatat gggtttgctt
    10681 ctagtcaaga aacaatccaa gttttgcagc catttgagat cgacacaaat ggtcagcaag
    10741 tggacttagg ttctcgaatc acagggtatc agcaaggtgc agcaggttca agttttcttg
    10801 aatggcaaga aggaaactgg cgtattcgaa tccgtgggaa taatatagaa ggccaagatc
    10861 ccttactatt agctaaagaa atcgtagctt atttggaaga aaacagtcta ccagcacctg
    10921 aacaatttgg taaaattact gttgatatgg gtgatacgac gaatcgagcc gtagaagtaa
    10981 gctggcaaga accgaaaaat gcctatacga tcactcatca agatcctatg tctgcgttga
    11041 agatggcagt ttctatgaag aggttgtgaa atcagcgcgc tgatctgagc ccatcattta
    11101 actccaagtc acaggaagta atcaaaaata gcacgccgca tttttgcata attttggctt
    11161 aataccttaa aagaaaacta gaattgcctc ttcacacatg ctaaaataaa acagacaaaa
    11221 aagaaatggg gatataagtt gactgaatac ttgaatgtag gaaaaatcgt aaacacacaa
    11281 ggtatcaaag gcgaagtccg tgttatctca acaacggatt tcccagaaga acgttacaaa
    11341 aaaggaacgg tgctaacact attccaagac ggaaaagcac ctgtggaatt aacagtcaaa
    11401 agtcatcgca aacataaaaa ttttgactta ttgagttttg aaggacatcc ttctatcaat
    11461 gatgtggaaa aatatcggga tggcatatta aaagtgtcaa aagacaactc tgtagatcta
    11521 gctgaaaatg aattctatta ccatgagatc atcggtctgc atgtattaga agatggaact
    11581 gagttaggta aagtaaaaga aatcttgtca ccaggtgcca atgatgtttg ggtcgttcaa
    11641 cgtccgaaaa aagctgatat attgcttcca tatatcgctt ctgtcgtcaa agaagtcgat
    11701 ttagaagctg gagtggtccg tgtagaaatt ccagaaggac tgattgacga tgaagattga
    11761 tgtattgact ttatttccaa gaatgtttga aggtcctatg ggggaatcca tcatcggtaa
    11821 ggctgtagat aaaggattat tagatatcaa tatctctaac ttccgcgatt attcagataa
    11881 caaacatcag acagtagatg attatccata cggcggtgga gcaggtatgc tgctcaaagt
    11941 ccagcccatc tatgataaca tccaagccat tgaaaaagct gcaccggatg tcaaaaaaag
    12001 agtaattctt cttgacccag ctggtaaacg attcgatcag aaaatggcag aagaattttc
    12061 acaagaagaa cacttagtat tcatttgtgg tcattatgag ggatatgatg aacgtatccg
    12121 cacattagtg acagatgaag tttccttagg agattatgtc cttactgggg gagaattagg
    12181 tgcgatggtc atgatcgatg caacagttcg gcttttgcca gatgtgttag ggaatcagac
    12241 ttcagctcaa acggattctt attcaaccgg tttattggaa catccccaat atactcgccc
    12301 ggcagaatat aaagggatga aagtaccaga agtattgaca aatggaaatc ataaattgat
    12361 tgaagaatgg caattaaaag aatcactaag aaggacctat caacgtcggc ctgatctttt
    12421 ggaaacactt gaatggacac ctcaaatgac gaaattttta gaagaaatca agaaagaaga
    12481 acaagaaaaa acagcagatg aagccaagtg actttttcta aaatcttctt tacagtaggt
    12541 tgataatatg ttagtatttt atggtgagtg caaaagcact taactattac gatattccgc
    12601 tgtgagaaaa gctcataaga atatttggaa taaggagaag aaaaccatga atccattaat
    12661 cgaagaatta acaaaagaac aattacgttc tgacatccca gcgttccgcc ctggtgacac
    12721 tgtgcgtgtt cacgcaaaag ttgttgaagg tactcgtgaa cgtatccagt tatttgaagg
    12781 tgttgttatc aaacgccgcg gtgctggaat cagcgaaact tatacagtac gtaaagtttc
    12841 taacggagtg ggcgttgaac gtacattccc attgcataca ccacgtgttg caaaaatcga
    12901 agtagttcgc tacggtaaag tacgtcgtgc aaaattgtac tacctacgtg cattacacgg
    12961 aaaagctgct cgtatcaaag aaatccgtcg ttaatcattg ttatcagact gagaaagaac
    13021 ccttggtatt caagggttct tttttcttag catgtaattt gaacaccctg atccagtttt
    13081 ttctttaatt attggtaggg gagaataatg gattagaata aatctaccta taagtagtag
    13141 taaaaaaaag agcactatga taatatcttt cttaagagta accgctttaa taaaaaatcc
    13201 agaatattct aacataaatg gagtattctt agaatcaaaa ggagaaagaa gatggtttat
    13261 acttatacgg agcaagaaaa gatgaaagtt ttagacacat tcatgaatga tggaaaagta
    13321 aaacaagtac caagttcaac aaaaaagaaa tacatcctat tgaaagaagt cttgaaacgc
    13381 tttgaccatg gagtgactta tacagaaaca gaggttaatt cgatactgct gaacgtattt
    13441 tctagtggcg attatgtcga acaaagacgt tatctaatca catttggctt tttcaaaaga
    13501 agttcagacg gtcgggcata tcagatgatg ggaattgaaa attagaagtt ttaatttata
    13561 ctcattgaaa aaagttcaag tatgcgatat tggttctaca ccgcatactt gaactttttt
    13621 tgtcatctgt atctataata caaagccaag aacgttattc aaaatatgga tagctatgat
    13681 aaaagctaat gagcgattcg cagttaaata agcaatcgtc agtaaaatcg ttccaggagc
    13741 gtacatgaaa aaatcaattg gatgcgttac atgaataaag ataattctct tttatttgta
    13801 cgaataaata ccaaaacgta cctcctaaaa ttagattttg gacagatgcc ctttaaaaag
    13861 ataacaagat ttcgttcttg aaacaatata tatgctttta ttccaatgag gttgtgaaaa
    13921 cagtattttg tcctgagcgt tagaaaattg aaaatgggga atagcaactc gtactttacc
    13981 attttttaag tgaatacaag atcagctaat tgaacaccgt ttattcgtga tgagggacac
    14041 aacactgcag tcgacacatt tctcttttat gttcaaagat aacaaatatt taaaacaaag
    14101 aaaaatagta gataatcccg tcctaactct tttattcttc cctcatagct tccaaattta
    14161 agcaatactt caattaatca agttttatct ttttttttca ataatcgtaa aaaaagagta
    14221 ttttttattt aaaaataaaa tctttgtatt aaaggacaag actaaaataa ccttagaata
    14281 aaaagagaaa aa
//
LOCUS       DACEUZ010000069        14269 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.069, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000069 DACEUZ010000000
VERSION     DACEUZ010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14269
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.069"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(299..1756)
                     /gene="ascB"
                     /locus_tag="IXG44_001699"
     CDS             complement(299..1756)
                     /gene="ascB"
                     /locus_tag="IXG44_001699"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAQ1658422.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(1753..3648)
                     /locus_tag="IXG44_001700"
     CDS             complement(1753..3648)
                     /locus_tag="IXG44_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1658423.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(3706..5481)
                     /locus_tag="IXG44_001701"
     CDS             complement(3706..5481)
                     /locus_tag="IXG44_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ1658424.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(5531..7027)
                     /locus_tag="IXG44_001702"
     CDS             complement(5531..7027)
                     /locus_tag="IXG44_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ1658425.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(7221..8843)
                     /locus_tag="IXG44_001703"
     CDS             complement(7221..8843)
                     /locus_tag="IXG44_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAQ1658426.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(9171..10484)
                     /locus_tag="IXG44_001704"
     CDS             complement(9171..10484)
                     /locus_tag="IXG44_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAQ1658427.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(10729..10953)
                     /locus_tag="IXG44_001705"
     CDS             complement(10729..10953)
                     /locus_tag="IXG44_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1658428.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(10973..11341)
                     /locus_tag="IXG44_001706"
     CDS             complement(10973..11341)
                     /locus_tag="IXG44_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAQ1658429.1"
                     /translation="MIQKTRVMLYVNDVEMICRFFVEKIGAEISETIELPEEFKSIVL
                     SISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETIYENMEEPGEITDNNGILT
                     FNFSDPEGNYFVIGKAESSD"
     gene            complement(11537..12247)
                     /locus_tag="IXG44_001707"
     CDS             complement(11537..12247)
                     /locus_tag="IXG44_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1658430.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(12265..12762)
                     /locus_tag="IXG44_001708"
     CDS             complement(12265..12762)
                     /locus_tag="IXG44_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1658431.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(12799..13251)
                     /locus_tag="IXG44_001709"
     CDS             complement(12799..13251)
                     /locus_tag="IXG44_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003331879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ1658432.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(13386..13991)
                     /locus_tag="IXG44_001710"
     CDS             complement(13386..13991)
                     /locus_tag="IXG44_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAQ1658433.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETSEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
BASE COUNT         4196 a         2978 c         2307 g         4788 t
ORIGIN      
        1 atcagaggtt gtgaaaaagc cgtcctgcat caagtaataa gaacagccaa acaaaaatag
       61 ttcctcatat tttgtcgttt tttgagctaa tgccgatggt cagctgattg tacaccgttt
      121 attgagaggt cgtgagcctg ccgttgaact ccgagtcaca aggagcaatt tcaaaaaaca
      181 gcttctcatg ttttatgact atggtgactt gtgatcgagg agttggcagg agaatactgt
      241 ttattcgtaa agaaggggct gtgacaagac ttttgtcaca gtcccttatt tccttttatt
      301 ataggatttc gccatttgat tggatgactt gtctgtacca atcaaatgat tttttcttta
      361 accgtttctt tgtgccattc ccttcgtcat cttgatcaac atagatatat ccataacgtt
      421 ttgacatttg tgaagtagaa gcgctgacga tatcaatgca gccccacgac gtataaccaa
      481 ataaatctac tccatctttg atagcttcct tcatctgctc aatatgctgc ttcaaatagt
      541 caatgcggta atcgtcttga acggttccat cttcttctac tttgtctata gctccgagtc
      601 cgttttcagc tatgaacagt ggtttttgat acctatcata taattgattc aaagcaattc
      661 tcaaaccctt aggatcaatc tgccagcccc attcacttga tggaagatac ggatttttaa
      721 ttcctccagt tattaagttt ccttggaccc cttcccaatt ttctggatga gcactgactg
      781 cactagacat gtagtagcta aaagaaacaa aatcaacagg gtaggctgct aagatttctt
      841 catcacctac ttctattttt aagttgacac ctttttcttc aaacattcgt tgcgtatacg
      901 ctggataata tcctcttgct tgaacatcac taaagaacaa agaagttctt tgctgctcaa
      961 ttgttttttg aatatcatcc ggatcacaag aatacggaac aagaagctga tagctgatca
     1021 tacaaccgac ttgtgaacct ttgataattt catgacaagc ttttgtagct aaagcacttg
     1081 ctataaactg atggtgtgca ccttggtatt tcgcttgttc caaatgggga tggttttctt
     1141 caattactcc tacgctaaca aaagggtgat gttttacaca gttgatctca ttgaaagtca
     1201 tccaatattt tactttattt ttatagcgtt tgaatactgt ttcagcgaaa cgaacgtaaa
     1261 actgaataag ctttcgatta ggccatccac catattctgt aaccaaatat aacggcattt
     1321 cataatggga aagagaaacg actggctcaa tcccatattt tagacattcg tcaaacactt
     1381 gatcataaaa aaataatccc gcttcattgg gtgtttcttc atcaccatga ggaaaaattc
     1441 ttgtccatgc gatagacatc cgatagcatt taaaacccat ttcagcaaat aaacggatgt
     1501 cttctttaaa gtgatgatag aaatcatttc cgtgtctttt aggataataa tacttgctgt
     1561 tgggatcctg cgccgctttt acttgctgat gcgtcatcat atgccattga tctgtccatt
     1621 tttctttagg taaatcgctg tcgaatgtat acacatcaga cacagataat cctttttcat
     1681 ctacatccca tgcaccttca cattgattgg ccgctgtcgc tccgccccat aaaaatcctt
     1741 ctggaaattt tttcatatcg atccccctaa acttgctagt tttcttgttt gattattttt
     1801 cttatgacta gtaatgatat ttttgaatct actactattt gtaacaataa ccattgtggt
     1861 cggatcatat ccctctttga ttaattcttg caaatctact tccatcaata aatcgccttt
     1921 acgtactttt tttccatcag atactgcact ttgaaagtat ttgccattta aattcactgt
     1981 atcaataccg atatggatca gtatctctgc tccttcttca ctgactaccc caattgcatg
     2041 tttcgttgga aaaactgttg aaacttcccc gtttacagaa ctgtagattt ttccgtccgt
     2101 tggaatgatc gcaaaccctt ctcctaaaga tccactagca aatgttggat cgtttacttc
     2161 agataaatca attatttcac ctgctgcagg agtacttaac actatatcct ttgaggattc
     2221 ttcttcgatt tcatccttgt agccgaaaag aaaagttagc atacaggaac tgatcatcgc
     2281 taagaaataa ctagcaacta gccaaacaaa tccttcacca atataaatcg ggaaagacaa
     2341 aatacttctt gtagcaactg ccaaagcacc ggctcctgcg gcaccagcta ctccaccaaa
     2401 tatagctcca catatacaag caatgtaaaa tggtcgttta tatttaagag tcacaccata
     2461 aacagcaggt tcagtgattc caaataatgc tgtgataaat gcagataaag ataaactttt
     2521 tactttctta tttttcgttt tcaaaaatac cccaagtact gctcctgctt gcgcagctac
     2581 ggcagtcata catgctggac ctagcgtatc acgaccgtag aaagaaagat tgttggtcat
     2641 tactggtaca agtccccaat gtaacccaaa aatcactaac acttgccaaa atccgccgat
     2701 aacagcacca gataaaacag ggctaaagtt ataaacagct aaatatccag ctgcaatcag
     2761 ctgacttgca taatctgcaa ttggacctac aactaataaa ataattggga ccatcacgat
     2821 gatacaaatg aatggtgtca ataaatttcg aatactttcg tgcaagcttt tgcgaatctt
     2881 aggctcaata taagaaagaa cccatacagc taaaattgct ggaataaccg tacttgtata
     2941 ccttgctaga atcacgggca tccctagaaa tcgcaaggtt atgctatcac tatatgcact
     3001 gacaatcgta ggataaacga gagctgctgc aataaccata gcgatgaaac gatctgtatt
     3061 gaactttttt gcagcagtat atgccaaaaa tataggcaag aaatagaaaa atgcatcagc
     3121 tgctgcattc aaaatgatat aagttcctga ttcagtagtt aataaattca aactcgtaca
     3181 taaggcgagt actcctttga tcaaaccact tcctgccaag acgcctaaag tcggggcaaa
     3241 aacagcggcg atcatgtcta taaaagagtt taaaatgcct tttttctcag ttcctctctt
     3301 tccactagat gcatcatctt ctagtttcat ttgtttggat aatacgtcat acacttcatt
     3361 tactgcattt cctactacga cttgaaacat tcctccagct tcgactacag tgatcacacc
     3421 ttctagttgc tctaccttct ccttatctgc cttcttatta tcttttaggg taaagcgtaa
     3481 ccttgttgca caatggtaaa cattgctgac attttcttcg cctccgacaa atcggacaat
     3541 atctttcgct aattgtgaat agtccatttt tacttccccc ttgttaatta atgccgtagc
     3601 aatccttctt acaagataag tatagttctt agaaaacggt ttctccattc aattagtacc
     3661 ccttctaata gcgaacgaaa ctgtacaata gtgtgtatgg tttacttagc cgagcaaatc
     3721 actcactgct tgaaagaaaa caagtatatc gtttctctta tagtcaaagg agacaaaaaa
     3781 aacacgcttg atcttccttt gctgatgtat aagtggtatc tcaagctgat actctgtgat
     3841 ttttgtttct acttggcttt tgataagaca gcacaacaaa gtctcggcat ttaacggaag
     3901 cataactagg tcagaactag gagagattag ttgctgaatg gaatctatct ttttatggat
     3961 ttttatttga ttttcttctt tgtagtaatg atcaagcagt tttgttttct ttttttcttc
     4021 aactatggtc agcttttcac gcaaaatctg ataaatctcc caagattcaa actcatctag
     4081 aacactaggt agataaatca aatcatccca ctcaaaaata agctcttgct cagtagtcaa
     4141 aattgcatca tattccgtta ttttgtttga ttgatttttg aaaagataca ctggttctat
     4201 ccggcattct ttgatccaca tgttcatact ttgttgaatc tgttttttta gtgtattcaa
     4261 aatactaatt ggctggttac taacaatcag tatctcccct tgttttcgaa aaggattcaa
     4321 cgcttcggca agatacaaaa caaaataagc gctttcagct aaattgggac gtaattgaaa
     4381 atgtattggg aagaaacgcc gaattaaata aatttctaaa ggataacggg agatgatctt
     4441 ttgtacaaaa tgattcatgg tattatgtcc ggcatccatc ctcgttttca tttgtttgat
     4501 atgggttagc aaaaattctg tttcatggaa caataacgaa acaggaagat ctaaaaaacg
     4561 aataatcgtt tgctcaaatg ctcttagatt cttctctatt tctttgtttt tttcatcatt
     4621 aatcatcaaa taatttaact ctaatagtcg attttcaatt gcttgttttt cttgattgga
     4681 aaaagaatag ggtagttctg atgataactt ttttgtgagg gtctctacca tcggaataaa
     4741 taatgtatac ctgtttgttt tttctaaaag ttcttctctt cgaagaatgg tcaaaacgag
     4801 ataccgagaa aatcgtatga agtcctctcc tgaaacaata taattatatg atataagaac
     4861 ctcttttaat gtcttttgga cagtcggcat gatattggaa aaaattgtac tgatttgttg
     4921 aggaaacctt gcgtgttgga tcaccgtttc tgttccaaca agagataaga aaattctttt
     4981 tgaattttct tcagcaatga tttttagtcc ttttgtcgac gaaacttcca gttctaattt
     5041 aggattgcgc tcgatccaac gcaaaatcgt ttttatttgt aatgatacgg ctgtttttga
     5101 aagaaagaaa atgtctgcta tcttttgcat actgacatac cctttttgaa acaataagta
     5161 cgtcacaata gttgaagctc ggaaatccat gctggaatag attttatgtt tcgtcacctc
     5221 ttgtatgatt tgctgtttag tttcagattc aattgattct aaaaaataac ctgaacgttt
     5281 atttgactgg ataatgattt tcttctcgta gatagcaagc atatttttga tttcagtttg
     5341 gatcgttttc ttgttggtac ctaaagcttc agctaattct tcgcttgtta tccaatgatt
     5401 cttatttctt aatactacaa gaatttggag ttgtcgttta tttaagtcca tttgcttaca
     5461 cctcctcttt gttataaaca tacaggaaaa acctctcaaa tgacaatagt cacttgcaga
     5521 ggtttaaaaa tcatttgaag aaagatctat attcaaggtt tttaccatta gactggataa
     5581 tttgtttata atattcaaaa ctatcttttt tcaatctcct aaagcttccg ctcccatcat
     5641 tttgtctatc cacataaata aagccatatc gcttcctcat ttcccctgtt cctgccgata
     5701 cgatatcgat tggtccccac cataagtaac caatgatttg gcaaccatct tttattgcct
     5761 cagcaatttc ctgaagatgt atttttagat actcacaacg ttttggatcg tggattcgat
     5821 tcaattcatc tggtgcttca tctagtccaa tgccattttc aactatgaat aatggtaatt
     5881 cataacggtc tgtcaattca ttcatgacat aacgaaaccc tagaggatca attggccaac
     5941 tccaaggttc gggagatttt tccttcaaaa agggattatc gaaaccattt gctccgccag
     6001 tatctacgcg catttctgct tcttttttat agacagcgct acgataataa ctaaatgcaa
     6061 tataatctgc cgtgttagct tgcaagcact gttcgtctcc atctaacatt actggtgcac
     6121 agtcttcttc cttccatgtc ctgtaaatat aagctggata atgccctcgg cacatgacat
     6181 cggagaaaaa ccatgaattg cgtttaaatt gcagtgaacc aaatacattc tctggatcac
     6241 agtcatatgc ataagtcgct aatgcagaaa agctacacat aatacccatc ttagcatttg
     6301 gatcgatttt ctttaataat tgagtagcac gggcgttcgc tagaaacata tgatgacagg
     6361 cttgataaat cacttttttt gttagtgtag caatgggttc aagtttatct ctagcaggga
     6421 atgtaaccaa tccagcagct gcataaggca tacgaaaagc attattgatc tcattaaagg
     6481 tcatccaata tttcactttc ccttggtagc gttcaaagac ggtctgcgca taaaattcaa
     6541 aaaagtcgat taattttcga ttttgccacc ccccatactc tcctaccaat gctaaggggg
     6601 tttcataatg actaagagta attactggct cgatatcata tcgatttaat gtattgatta
     6661 gtctgtcata aaatacgaga ccagcctcat ttggcttctg ttcgtctcct cttggaaata
     6721 ttcgtgccca tgagatgctt gttcgaaaag cttttaaacc taactccttc agtaaacgaa
     6781 tatcctcttc aaatcgatga taaaaatcaa tcgcttcatg agaaagataa tggagtgatt
     6841 cgtctaaagc aatctcccaa atcttttttt cttcattcca ctgaatacta ggatgctggt
     6901 tcatgatccc gataagcgta tctgtcacat taggtaattt gccgtcttcc aaccatgcac
     6961 cttcacattg attagcagct actgctccgc cccataaaaa attttctgga aatatttcct
     7021 gatccataac tatctctcct cgctatcaac aatttcttac ttgaagtata gctcgattca
     7081 tcaaaacaag atattctaac tatttcggca gaagagaaga aaaaaattga atgctactct
     7141 tcatttttaa tatttatata taacaaatac ataagacgat ccccgaaaga aaacactcgt
     7201 taaccatcta agcaaacgat ttattttact tccaacacaa atgcttcata aggtcgtaaa
     7261 tcaactttgc cgtctggtaa ctgtttcaga ggataatttg acacaataac ccttaaagct
     7321 tcacgtggaa tagtgaacgt ctgcttttca ctggacatat tacaaataac gagccattgc
     7381 tggtcttcat atatgcgaga ataggcaaag acatgctcag gcgtcttttc cactaattca
     7441 tagtctcccc agacgatgat gtcatgtttt ctacgcaaat cgatcaattt cttgtacata
     7501 taaaaaacag aatcttgatc tgctaacgct tcttctgcat tgatttcttt ataatttgga
     7561 ttgactcgaa gccatggaat tccagtagta aacccagcat gttcacgggc gttccattgc
     7621 ataggcgtcc tagcattgtc tcttcctttc gccttgattg attccaagat ttcctctttt
     7681 gtaaatccag aagaaatcct ttcgttatac atattgattg tctcaatatc ttttgcttcc
     7741 gcgatatcat cgattgggca attcgtcatg cctaactctt ctccttgata gatataaggg
     7801 gtgcctttca tcatatggag caagatagca aacatcttgg cactttcttt tcgatacaca
     7861 tcgtcatttc cccaacgaga aacaattcgc ggaagatcgt gattattcca aaataaacta
     7921 ttccatcctt catcacctaa agatgtttgc catttggata atactttttt cagttcagag
     7981 atactcaagg gtttcaaatc ccatttgtct ttgccttctt gctgatctaa tcctatgtgt
     8041 tcgaattgga agaccatgga taattcattt cttttaggat tggaataaag tttagcgatt
     8101 tcggtagttg ctccccatgt ttcgccaaca gtcatcagat ttttgtcacc aaaagttgct
     8161 tgattcattt cctgaagata ctcatgcagt ttaggtccat tacttgtgat ttctttatcc
     8221 ggttctttgc caatcatatc gatcacgtcc atacgaaagc ccccaatacc tttatcgatc
     8281 cagaaattca tcatttgata aatctcttgg cgaacctgtt tatttttcca attcaaatca
     8341 ggttgacgct tgctatacaa gtgcagaaaa tattgccccg ttttttcatc caactgccaa
     8401 gcagaaccac tgaatattga gcgcaatcta ttaggctctt ttccattgac cggatcacgc
     8461 caaatataat agtcccgata aggattttct ttatttttac gggcctcaat gaaccacgga
     8521 tgttcatcag aagtatgatt caccaccaga tccatgatga tacggatatt tcttttcttt
     8581 gcttcttcaa tcagttcttc catttcttcc atcgtaccga attctgccat gattgcttga
     8641 taattactga tatcataccc attgtcatca ttcggagact cgtatactgg agaaagccaa
     8701 attgcatcaa tacctaaata cgccagataa tccaacctgg aaataatccc ccgcaaatct
     8761 ccaataccat cttgatttga atcttgaaaa cttcttggat agatttgata aacaactgaa
     8821 tggtgccacc atttttgttc catgattttc ctcctataaa tggatgtttc tattttttgc
     8881 ttaatcattt attttagtct ctctcactaa aaattgtaac gctttcaaat agctattgca
     8941 ccttgttatc ggtaacagaa taaaacaaaa aaacagctga ggaaattccc tcaactgttt
     9001 tttgaaagga ttgatttatg cagggaagaa tgctgatttc acaacttctt tataaacggt
     9061 gttcactcag ctgtcccttg gtattagctc aaaaaacggc aaaatataag aaactatttt
     9121 ttcgtttttt tctctaatac tcaggccaga acgctgattt cacaacctct ttatttcaca
     9181 ttcaatatcc acatgaatcc cacaaataca aagaacagac catacatgat tggagatact
     9241 tcttttcctc ttttagctgc taacatggta attggataga agataaaacc taatgcaatc
     9301 ccatctgaaa tactataagt caaaggcatg ccgatcaaga tcaagaatga agggatcgca
     9361 atctctaagt ttccccattc gatttgtcgc aaagattgtg ccatcaatac tcctaccata
     9421 atcaatgctg gtgcagttac ttgtgaagta acaactgata gtaatggaga aaagaacaag
     9481 cctaacaaga agaaaatccc tgtagtaata gctgtgatac ctgaacgtcc tccaacggcg
     9541 ataccagctg atgattctac aaatgcacca acaggagaag taccaaataa ggaacccgca
     9601 agcatagctg ttgaatcaga agctaaagct ttccctacac gaggcatctt gttgtctttc
     9661 atgaaacctg cttggtttgc taatccaact aaagttcctg ctgtatcaaa gaaagtcaca
     9721 agcaagaatg tcagcactac gacccacatt tgaagagaat tgatgtcccc tacatgtttt
     9781 aatgcaacta aaaatgttgg tttcaaactt ggtgctgcgg caacaatttc agaaggcatt
     9841 ggaatcaaac ctgtgaccag acctaaaatc gttgtagctg ccatcccgat gaagatgcca
     9901 cctggtacac gccgaacaag caagatagca gttacgacta atccaaaaat cgtcaaccaa
     9961 gtagaaccaa cgttgagtga tcctaatcct actaatgtag attcattcga aacgatgatc
    10021 ccaccttcgc tcagaccaag gaaagcgata aatagaccga taccgccaga aatcgcatat
    10081 ttcaaatctg ctgggatcgc atcaatgatc atttcccgca gcttgaagat tgtgatcaaa
    10141 ataaaaatca aagaagcaac gaatacgccg gctaatgctg tttcccatgg aatccccatt
    10201 cctaaacaaa cagaataagc aaaaaaagca ttgatcccaa gagcaggtgc cgttccaatt
    10261 ggataacggg ctaatacccc cattaaaaca cagccgatcg cactggctaa tgcagtcgca
    10321 gtaaacactg ctccttcatc cattccagaa gcacctaata cagttggatt tacaaataag
    10381 atataagcca ttgagataaa tgttgtaaat cctgccagta cctcacgttt taccgtggtg
    10441 ttcaattttt ctatttcgaa gtaggaaagt attttttcct tcatcttcca tccccctaaa
    10501 tccgaacatt ataaataaag tccgttaaaa tttcgattta ttaataaaac aacgtgtttt
    10561 atcatatact aaaacaaaac ataagtaaac aaaaaattcg gattttaagc aaaatccgaa
    10621 cgttccatct gattaaatcc ctttatctca aagttcatga acattcactt tggattacaa
    10681 gaatttcttt attgtgttaa taaagtttct tcattctagt ctcaaaaatc aatagtgtga
    10741 tttgatttta ccatttcgtt tctgttcttc tttgatgaat tcttttgtat cgacaggatt
    10801 cagattattt agtccgccaa aacctgtatc gaccattttt ccccagcctt ttgtcttctt
    10861 ttttcctgat ttgagattct ttgctcgaag cttctcacta tgttttgctc gccgatactt
    10921 caaataacct tctaatcttc ccattagatc cactgttttt cctccttttt attcaatcag
    10981 acgactcagc ttttcctata acaaaataat tgccttcagg gtcagaaaaa ttgaaagtca
    11041 agatgccatt gttatctgtg atttctcctg gttcttccat gttctcgtag atagtttcaa
    11101 attcatccgt aaaaaatact aaagaaggcg gtggtcctaa tacctctggt gaaaacttct
    11161 gcacaaatac ttttgggaat acgcctaatt ctaattcttt tgagatgctg agtacaatac
    11221 tcttaaattc ttctggcaat tcgattgttt cagaaatttc tgcaccaatt ttttctacaa
    11281 agaatcggca aatcatctct acgtcattga cataaagcat cacacgtgtt ttttgtatca
    11341 tcatttgacc cttctttcta tttgattttc tttcctgtct ataaactgat tccattatat
    11401 cacgaaagcg caaacacctt gtgtttgata ctattcaaga aaaataaagc aatagtttct
    11461 aggattgctt tcctccacaa acttttttta caaaaaaaaa acggcaatga atgccgctct
    11521 tcttatttcg attcttttat aattctgctt ctgattcatc tttggttagt gtgtgtctaa
    11581 cactagttac gccaaatgtt tgatcgattt tttccttcaa cgcttcgatt gcctgtgcgc
    11641 cacgtgccgc ttgttcagca ttgatggatt caatgaccag caccgctgct tgtgtttctt
    11701 ccgtcagcat cgttctttgg gcttcacgcc agttgagaca gcgacagatc gctccttgtt
    11761 catcatagta gatgatttct tccggaagtg ccggagcatc ggaatctgca cctaaaggca
    11821 ggaatgattc gccaccttgg gcttttccta aatgcagatt tcctgcgatc ttttcgatat
    11881 cttctccgcc gcaaggtacg ccaaaagaca aggaaatact attatagata tctacaagcg
    11941 gattgattgg gaaaaattcc cgttcttgat ttgcacgttt cagtaaagct tcaatagaag
    12001 aacgtgcacc ttttttagtc ttgaatgtgg taaatgcctg acgccactga gcaatgactt
    12061 cgttttggct gaatgtttct tctgtaaggt aatttcttgc ttcttttgct gcctcattca
    12121 ataattgatg atacgtttct tcactttctt ttgttacgtg gttatcgata ccatttacgg
    12181 ataagatttc gatggttgct tcaggaaata cttcccagaa attggtatcc actattactt
    12241 ttttcatctg ttcacacctc ttgattagaa taattgcttg atgaatagat agtctgtttg
    12301 gggatcatct cccatataaa aaacatgttc gctgtatttg ataaatcctt ttttacgata
    12361 aaaagcgagc gcaacatcgt tgtgttccca aacaccgagc caaatggctg ttttttgctt
    12421 ttcttttgct ttttcaatag ctgtatctag taaaaatgta ccgatacctt gatgtttaaa
    12481 tttttttcgt acatagattc gctcgatttc taaaaaatga tcagccattt cctcagattg
    12541 actcgttcct gtatttagtt tcaggtaacc cgcaggctct tgatcgatac tcactaggaa
    12601 aaaatcggtt tctttatctt ttagctcttc tgtaagtttt tctagtgcat aagcttgtgt
    12661 caagtaatca gctaaatgct ccggtgaatt atcttttcca aatgtatctg taaatgtttc
    12721 gatactaatt tgttgtagta agtctacatc tgaaggcgtc atttttcgaa tttcgtattt
    12781 attcacggct tcttcctttc aatagatcct tttattgcct tttttgacaa aattccaatc
    12841 ttcgctaaca ttcttgcata ctttatttaa aagatattcc aattgttttg cttcttcgtc
    12901 agaaagacca tttaatgcga ctgcattgga gtagttgttt tctcgaatga tgaaaggata
    12961 gatttctgct ccttttttag tcggatagag tttcttgatt tttttatttt ctttatcttc
    13021 caaacgttcg atcatcccat tggattctaa tttcttgatt gctcgggcag tcgttgtacg
    13081 atctactttg atcatttctg ctagtttttc ttggatgatt ccaggatttt cgcaaatacg
    13141 tactaaataa agatactgtc ctcttgttaa atcgacttct ttgaattcga tattgctaat
    13201 agaatctagc gctcgggcaa tcattccgat gtctcgaagg atttcagcca tttcatccca
    13261 cctttatttt gtttgtaaga ctagttcacc tctttttgtt gcaaatgcaa ctattttcaa
    13321 taaaaaaatc aagttctttt ttaataacta gtaaagtata ttcttttctt cttttacgaa
    13381 aaagctcaat cacaatagat ctggtttttg ttttctaaaa taagtaactg tttcttcgtt
    13441 gttcgctttt ctgatagctt tggcaactct tctatactaa aaaatcccat gttggaagtt
    13501 tcattattct caataaactt ggcctcttca ccgtggatcg cacaagcaaa aacaagttta
    13561 taatactgaa aaagctgtgg aatatctttt tgtttattcg tatcaaaaac tgctcgcaac
    13621 tgagtagttt ctactgttaa tcctgtttct tcatagactt ctttacgcac attttcttca
    13681 ggagacaatc ctatttcagc aaatccacca ggaagtgacc attctttcgt ccgtttatct
    13741 tcgattagta ggaccttgcc atcttttttg atataggcac gaacatctac ttttggtgtt
    13801 ggatgtcctt ctccaatcgt tgtgagcgct tttatttctt cggaagtttc ttttcctatt
    13861 tcactgatca gttccaatga aatcgtccgg atctcctcaa aacgctcttt atcaaataca
    13921 tcttttccat aatatagacc agcttgagaa agtgcgagta gtcttttgta ttgagccaac
    13981 ttcttgttca ttctcttccc ttctttttta gcaaatgtat gactatgtaa tcaagtatag
    14041 aagactggtt taattacggc aaataaatat aatgaaatct gcaataaata aaagaatgtt
    14101 aaaaaactcg agaacaaaag acattcgact tctctggggc tgtgaataaa tggcgttcaa
    14161 aagcttgcat ctgcggtaat aagccccacc aaacgaaaaa tatgacgagc tattcttgtc
    14221 tggctgttct tattaattga ggcaggacag ctttttcaca acctcccca
//
LOCUS       DACEUZ010000070        14211 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.070, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000070 DACEUZ010000000
VERSION     DACEUZ010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14211
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.070"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            <1..407
                     /locus_tag="IXG44_001711"
     CDS             <1..407
                     /locus_tag="IXG44_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1658434.1"
                     /translation="LSGCSSNKKSEAKEDNSLLQPGTVWKEEVGGLVNNLKIIDDTTW
                     EYSESVWHPEQVQITVEKLKDYKGLERYKVIDSGGIDLFIGKTALIVPYNKDGLETFK
                     FIPTNEKEHRDKSEILKEYSTKSGYKLQKTSE"
     gene            404..646
                     /locus_tag="IXG44_001712"
     CDS             404..646
                     /locus_tag="IXG44_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1658435.1"
                     /translation="MIASLFIYPEGGITVAKAKTPFEKAASKILERRGVSETQWKQEV
                     LEKAQFDLLRGKDKDLEGYIYQREREKLVFDEIIKH"
     gene            664..966
                     /locus_tag="IXG44_001713"
     CDS             664..966
                     /locus_tag="IXG44_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1658436.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAAAA
                     VASIVIGGLFFLFGRRGAENGKMHVGYVLMGVGVILAAASIVVSLFGLFGQTPQGL"
     gene            1036..3090
                     /locus_tag="IXG44_001714"
     CDS             1036..3090
                     /locus_tag="IXG44_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1658437.1"
                     /translation="MYFTIAGNTKTDKELIKKATKEVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGKVLEHESRSSVKLTLALHDTEGKIEKTGTEYVFGPLDYQISEGFYPTLMDLIR
                     LDVFKETDETMSVEESYRNEKLLQVLEEELESDQRKVNQGSEVEVNEEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETAEVEVDPVEA
                     NEESTWTEGDSQEYIEPLISEEVEKNEIEIQKATVFDYILDQYPPEHEWTKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKAKEELNKEEQRVIEEEVQKIKERYEKKRDNAYSLCVE
                     KINNFTQTNQEALSKEKENLLKERIKERKEEFYLGLRTDKLKISKELNEQLNKLYQTT
                     TKVLMEKHEQIQQELQVQIPKWKKEYEIQQQELKEQEEKQRKKEKEHEQLEIRKREQD
                     LAEQKMAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPVAAVQTPVNNQ
                     ANLTPSPQRSFDGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEEAQE
                     QARKEYEQQLEELLNRTNQSTTESNMENSKESDSSKTKEGSSDSTVQSNTSESSESNE
                     VK"
     gene            3090..5699
                     /locus_tag="IXG44_001715"
     CDS             3090..5699
                     /locus_tag="IXG44_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HAQ1658438.1"
                     /translation="MRIRETTKRKDKPFNLEKKKYLSHFYSYAPAGERLPGKHDKRVK
                     QKLMYSGLVYLPLDYLVTRIPEAFSRFEIIQGSLQGVNLFQLQEMPQVFHFDSAGFWN
                     PLTPATSLGTRLVVSTICVVPAVLMGLKMDMWFRPLADGQKGDNRLMTLKEIQQIYPQ
                     IPDAKGSFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     KQSSMVVNDPKNELYVASKDELEARGYDVYAYNLTEPLQSMSDNPLALIVKYWQRGDV
                     DTATQLANTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYQDPTNEFKQLNALDEFMNQLPPGNLAKKQYGATKIGSDKAKGNIL
                     STAITGLNPFTLTKIAKMTSQSTIDYKSVGFPKYLDLKLHESLLNQRIVVEFYHQGKK
                     FHEERVKVGYRGFCEINFDCHLEQGDQVRLKYDHQRKEYVAWYEFSQRELRDEEGNIV
                     YRKKRGETHLPEWEKEAVLTLDESKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHII
                     VSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNMLW
                     DLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENST
                     TNSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNHG
                     ETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDIYARRLYHNMH
                     DQIEEEAKENIFIKIVQSLDNSTAQKLLIQFYEEKQIDRLQEYLEKLHQNEEITEVIR
                     KRLLLEAEKAVKE"
     gene            5696..8092
                     /locus_tag="IXG44_001716"
     CDS             5696..8092
                     /locus_tag="IXG44_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1658439.1"
                     /translation="MIIGGIFSGIANWWSGLFGDGDADPKTVTKVLQDYGNYLQYQDV
                     FSWICNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNELFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKIKNILVNLFMAMILIFGGSSLIDEGLNISKNFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLETDPNKVESLPTDKRNALTAKNFKTANINSVII
                     PKEAAKMADEDKSGLSDIQKDRLRALQYRLELDDNGKEIPVEISDEGLAKYVYTSGYR
                     RYISSPGIILAGESSLSVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSA
                     IEDISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQ
                     SVTKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSNLNDIRKNGF
                     GNRKDVIPDNGEEQPDDKATIVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGT
                     AEDLTEKAKDTLEEKLDGTRAKELAEAVKNPSEALEKQKEKASEVKEGLKDAYGEGQV
                     NAAVKANTTSEKENQEGAAIPSAKDHQDELHSNENSIGEKDSEEGQHGQLPKTDPEQY
                     RYKSNINSENEKRISDLPKRNQEVQTDEDSIDLPNDNGIEVGTEKSMKIGLPKTQNLP
                     MKDSRLNQFKENDQSFVHQSTAGTVQMKPSRLPENGNLTTVSYNLSESGETGNVSIQN
                     VVTREAAPEPIQKVHTGYKPANVAFTAPTKINVTRETKNRNNELKEQLLRDIKQDIKP
                     "
     gene            8144..8476
                     /locus_tag="IXG44_001717"
     CDS             8144..8476
                     /locus_tag="IXG44_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1658440.1"
                     /translation="MDKYINMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKIVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFADKNHYHALDPNRNY
                     HGTYKEDE"
     gene            8477..9070
                     /locus_tag="IXG44_001718"
     CDS             8477..9070
                     /locus_tag="IXG44_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1658441.1"
                     /translation="MRVKGQNSVDKKSILQFGKPEERSILDLFPYKSCTEEGVLVTKE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVQTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLRKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            9233..11257
                     /locus_tag="IXG44_001719"
     CDS             9233..11257
                     /locus_tag="IXG44_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAQ1658442.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASRLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTDTQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKRFLAMIDECHNAINPDFIETVNYVTNFQ
                     REMRKVEAMTVLATQSPQEMVPENMSSDNFSKLKKVFEFSSTKIFMRMDESILRHIEG
                     LVGKSMTNSELESIPQQNQGDAVINFGSKETIRVHFSPNKRQLKLFDGGK"
     gene            11257..12462
                     /locus_tag="IXG44_001720"
     CDS             11257..12462
                     /locus_tag="IXG44_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAQ1658443.1"
                     /translation="MENQDESLIKQYVKRRAKRRLFLWLFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYK
                     LLPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKF
                     PNSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGCMS
                     IITSNKLTEYDEFAIKHWGEGGNDNGTITGEWTNPFPGSSLDKSSFSGGQLFGTNPGG
                     EFRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVY
                     QEFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFTDDGTWIDP
                     LPFLNSSKK"
     gene            12478..13122
                     /locus_tag="IXG44_001721"
     CDS             12478..13122
                     /locus_tag="IXG44_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1658444.1"
                     /translation="MENKTLKIVLTAALSISLIGNIALFWQLKEEQATNTYKIEEVAK
                     NASEKLKKNFSEAQAKIDKLNQTINEKNQEIDQLKQQDGIKAKEEVTDAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPADDDHQHDDYGSYSFTLGDVQTYAETS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVTDFSYFTR"
     gene            13133..13939
                     /locus_tag="IXG44_001722"
     CDS             13133..13939
                     /locus_tag="IXG44_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1658445.1"
                     /translation="MSKKKGARQRFKKRKSYLRWLGIFTILGFLILASPIYTHKKVNI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQGLLKELANLDYRVEVKTAK
                     GDYQAIKAKVIKVSDDYFVVKLAHVPEEYIAMRLTIVPEKIDPKVDMQEPQDLIYYVH
                     EDKVKDQVKDDNYEQHAINYKVKGYKKEQKAAQKQIESLQATIDLNEELVNKLKDQLP
                     YQVADDQENTENKINDYQQEIETSKTQMKDQEEIIQKIQEKIDRMTKENI"
     gene            13962..14192
                     /locus_tag="IXG44_001723"
     CDS             13962..14192
                     /locus_tag="IXG44_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1658446.1"
                     /translation="MKGANLVGKDIFAEQKVLIKAYLKVVKAKEICQEVNLSNTTISE
                     LKNGRKSLDTLKFRSFEALYLAAKKYFTAERK"
BASE COUNT         5219 a         2214 c         2971 g         3807 t
ORIGIN      
        1 ttttaagtgg atgttcaagt aataagaaat cggaagctaa agaagataat agtttattgc
       61 aaccaggaac tgtttggaaa gaagaagtag gtggtcttgt taataatttg aaaattatag
      121 acgatacaac atgggaatat tccgaaagtg tttggcatcc tgaacaagta caaataacag
      181 ttgagaagct aaaagactat aaaggattag aaagatataa ggtaatcgat tcaggtggaa
      241 tagatttatt cataggtaaa acagccttaa tagttcccta taacaaagac ggtttagaaa
      301 cgtttaaatt tattccaaca aacgaaaaag aacatcgaga taaaagtgaa atattaaaag
      361 aatacagtac taaatctgga tacaagttac aaaagactag cgagtgattg ctagtctttt
      421 tatttatcct gaaggaggaa tcacagtggc aaaagctaaa acgccatttg aaaaagccgc
      481 gtctaaaatc ttagaacgcc gcggtgtatc tgaaacacag tggaaacaag aagtccttga
      541 aaaagcacaa tttgacctat tacggggaaa agacaaggac ttagaaggat atatctatca
      601 acgtgaacgt gaaaagcttg ttttcgatga aattattaaa cactaaaagg agcgaacaat
      661 tttatgtttg aatcaattac aatgaatttt ttaacacaac cgaaagtagt agctgcggtt
      721 gatattttta gagcattaca aactgcagca actagtttaa aaaacaattt gatgattttt
      781 gctgctgctg cggccgttgc ttccattgtt attgggggtc ttttcttcct atttggacga
      841 agaggagctg aaaatgggaa gatgcacgta ggctatgtat tgatgggggt aggagttatt
      901 ttagcagcag cttctattgt ggtttcgtta tttggattgt ttggacaaac acctcaagga
      961 ttataagaaa gtaaaaaagt aggagtaaga agtagtcgat actttttact ccttttttat
     1021 gaaaggagaa tactcatgta ctttacgatt gcaggcaata ccaaaacaga taaagagtta
     1081 ataaaaaaag caacgaagga agtaggaaag cgtacttctt ttttaccgaa aacaattaaa
     1141 tattctgtca ttgaaacgga agaacagcta catgtgttag ggaaagtact ggaacacgaa
     1201 agtcgatctt ctgttaagtt gactctcgcg ttacatgaca cagaaggaaa aatagagaaa
     1261 actggtacag aatacgtttt tggcccactg gattatcaaa tttcagaagg tttctatcct
     1321 acattgatgg atttaatccg ccttgatgtg tttaaagaaa cggatgaaac tatgagtgtg
     1381 gaagagtctt atcgaaacga aaaactttta caagttttag aagaagagtt agagagcgac
     1441 caaagaaaag tgaatcaagg gagtgaagtc gaagtaaatg aagaagtacc atgggaacga
     1501 ccaatatttc caaagaagga gtcaaaagaa aaagagacag tctatgttga cgaacaagct
     1561 gatattccga ctcagaaatc gatatttgag gaagagaacg atgtgatggg tcaattggag
     1621 gtagatccct ttgaagaaac aatggaggat gaagagacgg ccgaagtaga agttgatcca
     1681 gttgaagcta atgaagagtc aacatggact gaaggggatt ctcaagaata tattgaacca
     1741 cttatcagtg aagaagtaga aaaaaacgaa atagagatac aaaaggcaac tgtatttgat
     1801 tatattttgg atcaatatcc gccagagcat gaatggacga aagaaaaaaa caaattattt
     1861 ttaaatgagc tttattcgaa atatcaatta ccccaaacct atcaagcatt tttagagagt
     1921 aaagaagaat taatctcaca gggtaaaggc acacttttag aacagttaga gtctgtccaa
     1981 tcaacaaatt ggcatgagct tgcacaagaa gatttattag ccacctttga agaacgtcaa
     2041 caagaaacag atcaagaaat taatgagtat gcgacggaac agtcgtctaa ttgggagaaa
     2101 gcaaaagagg aactaaataa agaagaacag cgagtgattg aagaagaagt acaaaaaatt
     2161 aaagagcgat acgaaaagaa acgcgacaat gcgtattcct tgtgtgtaga aaagatcaat
     2221 aattttactc agacgaatca agaagcgtta tcgaaagaaa aagaaaactt gttaaaagaa
     2281 cgtattaaag aacgaaaaga agaattttac ctaggtttgc gtacagataa gctaaagatt
     2341 agcaaagaat taaatgaaca actgaataag ctatatcaaa caacgactaa agttcttatg
     2401 gaaaaacacg aacagatcca gcaagaatta caagtacaaa ttccaaaatg gaaaaaagag
     2461 tatgaaattc aacaacaaga attgaaagaa caagaagaaa aacaacggaa aaaagaaaaa
     2521 gagcatgaac aattagaaat aagaaaacgg gaacaggatc ttgcggaaca aaaaatggcg
     2581 atagaaaaac aacggttaga gcaagaaaag gaaaaagaag cagctgctcg taaggaacgt
     2641 caagaacaaa tggatacatt gcgtcaagca cagttgaatt atctagcaca acctcaacca
     2701 gtagctgctg ttcaaacgcc agtgaacaac caagcaaatt taactccttc accacagcgt
     2761 tcctttgacg gatggatggt gggatgtatt gcaagtttaa gcttgcttct aggtggaggg
     2821 acagtatttg cttttaatca tatcaatcaa tcttcacagg ctgattctgt agccacaacg
     2881 gcgtcaatca aggaagaagc ccaggaacaa gcaagaaaag agtatgaaca acaattagaa
     2941 gaattgttga atcgtaccaa tcagtcaaca acagagtcaa atatggaaaa cagtaaagag
     3001 agtgattcta gtaagaccaa agaaggatca agcgattcaa ctgtacaatc aaacacttcc
     3061 gaaagttcag aatcaaatga ggtgaaataa tgcggattcg agaaacaacg aaacgaaaag
     3121 acaagccttt taatttagaa aaaaagaaat atctatcaca tttttatagc tatgcaccag
     3181 caggtgaaag attaccaggt aagcatgata agcgagtcaa acaaaaacta atgtatagcg
     3241 gtttagtcta tctcccttta gattatttag tgacaagaat acctgaagct ttcagtcgat
     3301 ttgaaatcat tcagggaagc ttacaaggag tgaatctgtt tcaattacag gaaatgccac
     3361 aggtctttca ttttgattca gctggttttt ggaatccttt gaccccagca acgagtttag
     3421 gtacacgctt agttgtttct acgatttgtg ttgttccagc tgtacttatg ggtttgaaaa
     3481 tggacatgtg gtttcgtccg ctagcggatg gacaaaaagg ggataatcgg ttaatgacac
     3541 taaaagagat tcaacaaatc tatcctcaaa taccagacgc gaaaggttct ttccctggat
     3601 atggtggaat tcctatcact cactataaaa aatattggta tatccaaaca gataccgtta
     3661 atacttgtat tgttggtact tctcgatcag ggaaaggaca gattgaagta ttagcaacta
     3721 ttgataattt gtctcgtgca gaaaaacaaa gttcaatggt tgtcaatgat cctaaaaatg
     3781 agttgtatgt agccagcaaa gacgaattag aagccagagg ctatgatgtt tatgcttata
     3841 atctaactga accgttacag tcaatgagtg ataatccttt ggctttaatt gtgaaatatt
     3901 ggcaacgagg agacgtggat accgctacgc aactagcaaa tacatttaca aatacgattt
     3961 attatgatgc caatgcttca gataacaaat actttaacga aaacgcgcag aaagctgtca
     4021 atgcgcttat ttttgcgttg ttagaacaag ccgatcaaac aggggattac tccaaattaa
     4081 cacccaataa tcttgttgag cttctttctg aaattggcgg ttttaattat caagatccta
     4141 ctaatgaatt taagcaactc aatgctttag atgaatttat gaatcagtta ccacctggaa
     4201 atctagcgaa aaaacaatat ggggcaacaa aaattggttc tgataaagca aaaggcaata
     4261 ttctatcaac agcgatcaca ggattgaatc catttaccct aacaaaaatt gcgaaaatga
     4321 cttctcaatc aaccattgat tataaatcgg ttggttttcc caaatatttg gatttgaaat
     4381 tacatgaaag tcttttaaat caacgaatcg tagtggaatt ttatcatcaa ggaaagaaat
     4441 ttcatgaaga acgagtaaaa gttggttata gaggtttttg tgaaatcaac tttgactgtc
     4501 atttagagca aggggatcaa gttcgtttaa agtatgatca ccaaagaaaa gaatatgttg
     4561 cttggtatga attttctcaa agagaattga gggacgaaga gggtaatatc gtttatcgaa
     4621 aaaaaagggg agaaacccat ttgccagaat gggaaaaaga agcggttctc acactagatg
     4681 aaagtaaaag taacttatta gtagagtcta ttcggatgca ctataccgat aaaccaatgg
     4741 ctatttttat gctcacacct gattatgatc catctaacca tatcattgtt agtatttttc
     4801 ttagtcagtt gtataaagaa ttatctacgc aatgtgttaa gactaaagga gataaatgtc
     4861 accgtaggat tcattttatt ttagatgaat ttgggaatat gccaccaatg gataatatgg
     4921 aaggaatcat gacagtcaca gctggtagaa acatgttgtg ggatctgttt attcaatcct
     4981 atcagcaact ttatgcgaag tatggggaac aaaacgcagc gattatcaaa gaaaattgtc
     5041 agacacatat ttatattatg tcgacagacg ataataccat cgaagaattt tccaaaaaag
     5101 tggggaataa gacggttgaa caagagaaca gtaccaccaa ttcattaggt atgaatactc
     5161 atatcaatcg caatgttgat tcagatcgta tcttaactcc tgaaagaatt tctacttttt
     5221 tagaaggtga aacgattgta ctcgctcctt taaaacgaca agatttgaaa ggtcataaaa
     5281 tacggccgta tcctattttt aatcatgggg aaacgaatct tccttatgct tatcaatata
     5341 ttgaagattt caatccatcg tgggatatca atgatataga catacattgt acacatgcgg
     5401 acttggatct taaggcttta caaattgatt acgattattt tctacctgat atctatgcac
     5461 gtcgacttta tcacaatatg cacgatcaaa tagaagagga agctaaagaa aacattttta
     5521 ttaaaatcgt tcaatcttta gacaactcaa cggcacaaaa gttacttatt caattttatg
     5581 aagagaagca aatagatcgt ttgcaagaat accttgaaaa attgcatcaa aatgaagaaa
     5641 ttacagaggt tataaggaaa cgacttttgc tagaagctga aaaggcggtg aaagaatgat
     5701 cattggagga atattttccg gcattgccaa ctggtggtca ggcttatttg gggatgggga
     5761 tgcagatccc aaaaccgtaa cgaaagtctt acaagattat ggtaattatc tgcagtatca
     5821 agatgtattc agttggattt gtaatacttt tttatggggt ttgattaaag ggttatataa
     5881 tctcaatact ttactagaaa aaacaattta tcaaagcttt gatttaaaag acttattgaa
     5941 cgctgctggt gttaatgaac tgtttcaaag tttgattagt aaggtattgg ctcttttttg
     6001 tgtcgtcaca ttaatttatt taggcttaaa attttttatg agtaaacatc caccaaaaat
     6061 caaaaatatc ttggttaatc tatttatggc catgattttg atttttggtg gtagttcttt
     6121 aatcgatgaa gggctaaata ttagtaaaaa cttttacgga gacgtgactg cagcaaataa
     6181 agcagataaa actggatcac ctgcttttca aattattcaa aataatgtga tcgatgtgag
     6241 tacgctacta gaaactgatc cgaataaagt agaatcccta ccaacagata aaagaaatgc
     6301 tctcacggcg aaaaacttta agacagcaaa tattaattca gtgattattc ctaaggaagc
     6361 cgctaagatg gcggatgaag ataagagcgg tctatcagat atacaaaagg atcggctaag
     6421 agcgttacaa taccgactgg aactggatga taatggaaaa gaaatccctg tagagattag
     6481 tgatgagggg ttagcaaaat atgtgtatac ttcaggctat cgccgataca ttagtagtcc
     6541 agggattatt ttagctggtg aatcttcttt gtcggttgcc tatttattta tcttgtttac
     6601 gattgtaact tgcattattg agctggtatt taaaaaattc tatttagtaa ttgccgcgtc
     6661 aacggacttt gagacaggac aacggatgaa aagtgctata gaagatattt ctcaatcctt
     6721 cttgttattg gcgtttacga ttttagaatt acgaatctat acattaatgc tgtcaggtat
     6781 cggtgatttg cacgcagcag gcaagatcaa cggatttttg tatgttgtcg ctttaattgt
     6841 actaaccatt gcgctattca aaggctctca atctgttacg aagattttcg gtgttgatac
     6901 aagtctgaaa aacggcagca attctcttct tagcatgttt gccttaggaa atattgcgaa
     6961 aaatgtgggt agtggggcta aaaaccttgc aagtgctggt aagagtggtt taagcaattt
     7021 aaatgatatt cgtaaaaatg gatttggcaa tcgaaaagat gttattcctg ataacgggga
     7081 agaacaacca gacgataaag ctacaattgt acctaatagt aagaaaacag tttccgaaat
     7141 gttcgggaaa gcgaaaaatg gtgtttctaa agccgcagaa ggtcttggtt acgtcaatga
     7201 acgtgggtta aaaggaacag ctgaagatct tacagaaaaa gccaaagaca ccttagaaga
     7261 gaaattagac ggcacgagag ctaaagaatt ggctgaagct gtgaagaatc catccgaagc
     7321 actagaaaaa caaaaagaaa aagcttcaga agtcaaagaa ggactaaaag atgcgtatgg
     7381 tgaaggacaa gtcaatgcag cagtgaaggc aaatacaaca agtgaaaaag aaaatcaaga
     7441 aggtgcagcg attccttcag ccaaagatca ccaagatgag ttacattcta acgaaaattc
     7501 gatcggagaa aaagattcag aagaaggaca acatggacag ttaccaaaaa ccgatccaga
     7561 acaatatcgt tataaatcta acattaattc tgaaaatgaa aagcgcattt cagatttacc
     7621 aaaacgaaat caagaagtac aaacggatga ggattccatc gatttgccaa atgataatgg
     7681 aatcgaagtt ggaactgaaa aatcaatgaa aattggtttg cctaagacac aaaatttacc
     7741 aatgaaagat agtcgtttga atcaattcaa ggagaatgat caaagctttg ttcaccaatc
     7801 aactgcaggt actgttcaaa tgaaaccaag tcgtttgcct gaaaatggaa atctgacaac
     7861 tgtttcttac aatttatctg aaagtggtga gacagggaat gtgtccattc aaaatgttgt
     7921 gacaagagaa gctgcaccag aacctattca aaaagttcac acaggttaca aaccagcaaa
     7981 tgtggcattt actgcaccaa caaaaataaa tgtaacaaga gaaactaaaa acaggaacaa
     8041 tgaattgaaa gaacaactat tgagggatat caaacaggat atcaaaccat aaaaggccta
     8101 cgagttaggc cttttttcta tgtatgaaaa ggagaaatca tttatggata aatatattaa
     8161 catgaaaaat cctccaagat ttagtgggga atggaagttt ttcgcctttt tctttttaga
     8221 tctattaacg cttggtggtt gctttttttg ggcgaaaata gtcaataatc ttgtccactt
     8281 accactcttt tttgcactct tgaattatgg cttatggatt gcgatagctc tagtgattgt
     8341 ttggcgacct tataataacc caggtcaacg acaaattttt gcgattatcg aatctatttt
     8401 ctttgcggat aagaaccact atcatgcgtt agatcctaac cgaaattatc atggtacgta
     8461 taaggaggat gaataaatgc gagtcaaagg acaaaattca gttgataaga aatccatttt
     8521 gcagtttgga aaaccagaag aacgatctat cttggattta tttccttata aatcctgtac
     8581 agaagaggga gtcttagtta ctaaagaaga tcattttcaa cgttattatc gtgtagtttc
     8641 tacagacgtg gaaggattaa acgaacaaga gaaactagaa cgaatgaatc agttgaccac
     8701 cgtcatgcgt acgtatgtgc aaacaattaa gttagttagt ttgaccacag agacggatct
     8761 aacggaacaa atcgtacaaa agcggcaatt attagataag aatcgtatgg aacaatcctt
     8821 aggaagaaat ctacagcgat taagaaaata tgaaaagaaa ttagtagagg aaatccagga
     8881 attgaaacga gcagaacaag aacgtcctga cttaagcttc tttttcgtca ttgaaggaaa
     8941 gacaatcaag gatttacaaa ccagaaatcg tcagttaatg cgatctagtg gcattttagg
     9001 gttaaaacca ctctacaaaa aagaattgat caagattctt tatcgaatga acaatatgaa
     9061 tgatgagtag gaatggagaa gagagaatga aatatccgtt gatatggtta gaaaatgatg
     9121 ttccgaagga agagatcact agaaaatttt ctcacacttt aagcctgggt aagttgggaa
     9181 aagggcaatc ttttaaccaa gtaaagaatc aaacaagaag aggtgactgt acatgttaga
     9241 agcgattaaa acagcttttt caccacaaaa tgatcaaaaa gtacttgatc cattaaagca
     9301 actaggaaat cgggaaatag aaattttaaa atccaaaggg tatgatttag attttttaaa
     9361 acaagtgatg ccacaaggtg gattaaattt cgatgaagag tatgtgacct atggaaatgg
     9421 ttattcacga tgtgtgacag tctatcacta tacaaaaaat cctactcttt tttggttggc
     9481 caatctcatg aacaatcctc atacagtggc cacactagat gttagcacag atgaaaagca
     9541 aaaagtgatt gatcaaatca accgcgcatt agatgaatta aatgatcgat cagataatga
     9601 acgaaaagcg actgaccgaa ataaaagtgc ggaagaatac ctggaactag cggaatatgc
     9661 ccgaaaattg acttctaatg gggaaatttc taaacaagtc aaagtgagag tacatgttta
     9721 cggaaatacg atacaagaag tggatgaacg ggcgcaggaa ctgatcgaaa atctaaaagg
     9781 gatggattat cgtgcggtga cttatctgca taaaaccaaa cgagattggc aatcgatgtt
     9841 taccatgttc gatgaacaag ctgaatggtt cggaaatatc aaagggcaat cactaccatc
     9901 gttaaacatt ggcggaggtt tcccgttcca ccaccaaacg ctaaaagatc caggaggtac
     9961 acaatatggg gaaaccatga caggcggccc atttgtctgg aatgcgacgt ataaaaatag
    10021 tgtacggttg tctactaata ccttgcttct aggaatgatg ggagctggta aatcgaccgt
    10081 tcttaaaatg atcgcggaag cccatttagc tgcaggagac tttatttggg gctttgaaaa
    10141 gggaaaagac tttattcctt tcttaaagga atataacggt attatggtgc gtttagacgg
    10201 atctgacggg atgatcaatc ctttagagat atttgccaca cggacgtatg atgaagcatc
    10261 caggttatat gacgatggat cagtcaaaga tttgaagatc aatgaagcag cgtcctatca
    10321 aacgcactta gacaaagtag tctatcaggt acagttagtg agtcctcagc taaaaggaac
    10381 gatgaaagca gaatttaaga cctacttaaa tcgtttttat gaagagtatg gaacagtacc
    10441 acgtggcttt acttcctcaa attcgcgttc aaatactgat acgcaagtca caggtaaaga
    10501 tccagaagct tatccgacgt tcaaagaatt tttagacttt ttaggacaac tggaattacc
    10561 aggtgcttcc caagagaaaa agaatcgaaa agaagaaatg gaatccatcg tagaatcctt
    10621 atgtgagacg tatggcatga tttttgatgg tcacagtacg attcgacacc ttgaccaaca
    10681 acagttggtc ttttttgaca ttgactctat tacaggttta gatcgagggg tgcaacaatg
    10741 tcaaatgtac atggctatga cgttgatttg gaatcaggca ttgatccagg gaagaagaca
    10801 acgacgttta attaaagaaa aacaaatgga agaacaagac ttcaaacgct tcttggcgat
    10861 gattgatgag tgccataatg cgattaatcc agattttatt gaaacggtaa attatgtcac
    10921 aaactttcaa cgagaaatgc gtaaagtaga agcaatgact gttttagcta cacaatcccc
    10981 acaagaaatg gtgccagaaa atatgtcttc tgataacttc tcgaaactga agaaagtctt
    11041 tgaattttct tctacgaaga tctttatgcg tatggatgaa tctattttac gtcatataga
    11101 aggactagtg ggtaaatcaa tgacgaactc agaattagaa tccattcctc aacaaaatca
    11161 aggagacgct gtgatcaact ttggatcaaa agaaaccatt cgcgttcatt ttagtccgaa
    11221 caaacgtcaa ttaaaattgt ttgatggagg aaaataatgg aaaatcaaga tgaaagttta
    11281 atcaagcagt atgttaagcg tcgcgcaaag cggcgcttat ttttgtggtt gtttggtact
    11341 tctgcaggtt tgattactat tttgatcact gtgtttgtta ccctatttct aatacttgcg
    11401 gcgggttcga ttgataattc ggattcagat tcaagcagtg gtggcgaagc ttttacagga
    11461 gaatattcgg aaggcttacc gatttataaa gaaatcaaag gaagaggccc tttttctgat
    11521 gagattgcgc aatatgccgt aggtgcagca gtgaaatata aattattacc aagtgtgatt
    11581 ctttcgcaat acggttatga atcagcgttt ggtacgtcgg cgtccgcaag aaatgatctc
    11641 aactattttg ggattacttg gtttgatggt tgtctgtttc ctaaaggaac tgcacgagga
    11701 attggtggca ttgaaggtgg ctggtacatg aagtttccca actcaaaagc tgcattctcc
    11761 tattacggct ttatggtagc cacacaatca aattttaatg catgtgtagg aaataaaagc
    11821 cctggcgcaa gcttattgat cttaggacga ggtggttatg ccgctgcagg aattactgaa
    11881 gatagtccgt attatacagg ttgtatgtcg attattacct caaataaatt aacagaatat
    11941 gatgaatttg cgatcaaaca ttggggagaa ggcggaaatg acaacggtac gattactggt
    12001 gaatggacga atcctttccc tggcagcagt ttagataaaa gttccttcag cggtggtcaa
    12061 ttatttggaa cgaatccagg cggtgaattc cgtccgaatg gtttccatga tggattagac
    12121 tttggttcag ttgatcatcc aggaagtgag attcatgcgg ttcatggagg aaaagtcgtg
    12181 tacgttggaa atcctggtat ttcaggtttg ggcgcgtgtg tgatcgtgat taattacgac
    12241 ggcttgaata tggtatatca agaatttgca aatagtacag gaaattcccg tgtgaaagtg
    12301 ggagatcaag tgaaagttgg tcaagtaatc gggatacgag atacagcaca cttgcattta
    12361 ggcttcactc gaatggattg gcgtcaagca caaggacacg cattcacaga tgatggtaca
    12421 tggatcgatc cattaccatt tttgaatagc agcaaaaaat agatcaggag aaaaaatatg
    12481 gaaaataaaa cgttaaaaat cgtcttaaca gctgcactca gcattagctt aattggcaat
    12541 atcgcgctct tttggcagct gaaagaggaa caagcgacaa atacctataa aattgaagaa
    12601 gtggctaaaa acgcgtctga gaagctcaaa aaaaatttct ctgaggcaca ggcgaagatc
    12661 gacaagttaa atcaaaccat caatgagaaa aaccaagaaa tcgatcagct gaagcaacaa
    12721 gacggtataa aagcaaaaga agaagtcaca gatgcccaaa gaaagacggc agaagagttc
    12781 ggtcaattgg ccattgataa atcactaagc agaaatgaat taacggaaaa actaaaagat
    12841 gtcgcaaccc aagaagtgat cgacaaatta agtcctgcag atgatgatca ccaacatgat
    12901 gattatggaa gttactcctt tacattaggt gacgtgcaaa cttacgctga aacaagcagt
    12961 gtaaaagagg aacaaaattt tgtcgtattt gttgattata caatcggcaa tcctcaattc
    13021 aaagatgtca aaccgcaaaa aataaaaggt ggattaactg tcactgaaaa acaagtagat
    13081 ggtcaatgga aagtcacaga ctttagttat ttcactcgtt aggaggaaga aaatgagtaa
    13141 gaaaaaagga gcaagacaac gttttaaaaa acggaaaagt tatcttagat ggctgggaat
    13201 ctttacgatt ctcggctttt tgattcttgc tagtccgatt tatacgcata agaaggtaaa
    13261 cataccagat gaggtaagca cgaacgcttt tttaaacacg aaagaactga caatggtatc
    13321 aaaagaaaaa ggagcgaatc agcaattagt ttttgaattt gcgattgacg atgaggatca
    13381 aggcttatta aaagaactgg ctaatcttga ttatcgcgta gaagtaaaga cagctaaagg
    13441 tgattatcaa gcaataaaag ccaaagtgat caaggtgtct gatgattatt ttgtagtgaa
    13501 attagcacat gtaccagaag aatatatcgc catgcgttta accattgttc ctgaaaaaat
    13561 tgatccaaaa gtggatatgc aagaaccaca agatctgatt tattacgttc acgaagataa
    13621 ggttaaggat caagtgaaag atgataatta tgaacaacat gcgatcaatt acaaagtgaa
    13681 aggatacaag aaagaacaaa aagcagcaca aaaacagatt gaatccttgc aagcaaccat
    13741 tgatttgaat gaagaattag tgaataaact aaaagaccaa ttgccttatc aagtcgcaga
    13801 tgatcaggaa aatactgaaa ataagattaa tgattaccag caagaaattg aaacatcgaa
    13861 aacgcaaatg aaagatcaag aagagatcat tcaaaaaatt caagaaaaaa ttgatcgtat
    13921 gacaaaagaa aatatttagt agaacaggtg gagtgaaaga aatgaaggga gcgaacttgg
    13981 tcggcaaaga tattttcgct gagcagaaag tattgattaa agcctatctc aaagttgtaa
    14041 aagctaaaga aatatgtcaa gaagtgaatt tatctaacac aacaatatct gagttgaaga
    14101 atggtagaaa gagtttagat accttaaaat tcagatcatt tgaagctttg tatttagctg
    14161 ctaaaaaata ttttacggct gaaagaaaat aaaaacagga ggattttatg t
//
LOCUS       DACEUZ010000071        14067 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.071, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000071 DACEUZ010000000
VERSION     DACEUZ010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14067
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.071"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(9..530)
                     /locus_tag="IXG44_001724"
     CDS             complement(9..530)
                     /locus_tag="IXG44_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAQ1658447.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            729..4421
                     /gene="nifJ"
                     /locus_tag="IXG44_001725"
     CDS             729..4421
                     /gene="nifJ"
                     /locus_tag="IXG44_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HAQ1658448.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            4610..5497
                     /locus_tag="IXG44_001726"
     CDS             4610..5497
                     /locus_tag="IXG44_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HAQ1658449.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            5494..6663
                     /locus_tag="IXG44_001727"
     CDS             5494..6663
                     /locus_tag="IXG44_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1658450.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            6687..8042
                     /locus_tag="IXG44_001728"
     CDS             6687..8042
                     /locus_tag="IXG44_001728"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HAQ1658451.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(8114..9919)
                     /gene="glmS"
                     /locus_tag="IXG44_001729"
     CDS             complement(8114..9919)
                     /gene="glmS"
                     /locus_tag="IXG44_001729"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HAQ1658452.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            10328..10618
                     /locus_tag="IXG44_001730"
     CDS             10328..10618
                     /locus_tag="IXG44_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1658453.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            10631..11656
                     /gene="sppA"
                     /locus_tag="IXG44_001731"
                     /pseudo
     CDS             10631..11656
                     /gene="sppA"
                     /locus_tag="IXG44_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
     gene            11659..12318
                     /locus_tag="IXG44_001732"
     CDS             11659..12318
                     /locus_tag="IXG44_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HAQ1658454.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            12475..13407
                     /locus_tag="IXG44_001733"
     CDS             12475..13407
                     /locus_tag="IXG44_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1658455.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            13561..>14067
                     /gene="tig"
                     /locus_tag="IXG44_001734"
     CDS             13561..>14067
                     /gene="tig"
                     /locus_tag="IXG44_001734"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624743.1"
                     /note="Tig; RopA; peptidyl-prolyl cis/trans isomerase;
                     promotes folding of newly synthesized proteins; binds
                     ribosomal 50S subunit; forms a homodimer; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HAQ1658456.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVID"
BASE COUNT         4516 a         2548 c         3096 g         3907 t
ORIGIN      
        1 ttattttttt attttgtttg gatcaattct tttaattcct ctattgaaat agattcatca
       61 aagatttctt tacccaagat gacagttgga acaaatcgaa tatgtgcttg ctcagcttcg
      121 ttgatgacag cttggctcag ctgttcgtct ttttgttctg tcagctttag tttatctact
      181 gcaaaatccg ctacttcttg gagagaaaga tgtttccact cgtcttgagt atcgaatatt
      241 tgtttgatct ctttgatcgc tttttggcca tcgctgattg tgagataccg gtgcatcacg
      301 ttccctcgct gtaagctttc tttttcttta tcgaacaatt tgatcactcg ctgcaattgc
      361 ccgctttgga ctgcttcttc taatagctca taagattctt caaaccattg tcggcaatac
      421 ggacaagcta agttgatgaa ttcgaccatt tttttaggag cattcgatga accataaagg
      481 attccttttt gtgtatttgt tttcgttgca tcaatcactg atatatccat gttaaaacac
      541 ctctttcgct tatagtttat acatgtctga caaaaaaagg caacgattta gactgtttgt
      601 ctaaaaatta gatgaccaat tcaaatagat acatgtaaaa tatttttata aataaaagaa
      661 aaaaatggca tgttttacgt tataatggaa cagataataa gagagaatat gaacaaggat
      721 gatgaatcat gcgaaaaatg aaaacaatgg atggcaatac ggcagccgca tatatttctt
      781 atgcatttac cgagattgct gcaatatatc caataacacc cagttcgacc atggctgaat
      841 tagtagatca atggtcagca gcaggaaaag aaaacgtatt cggtcaacca gtaaaagtag
      901 tagaaatgca atccgaagca ggagcagctg gtgtggtcca tggatcattg aaaactggtg
      961 cactgacaac tacatatacc gcttcacaag ggctgctttt gatgataccg aatatgtaca
     1021 agattgctgg ggaactactg ccaagtgttt tccacgttgc tagtcgggca gtgacgacaa
     1081 atgcgttgaa tattttcgga gatcacggag acgtgatggc tgcaagacaa acaggatttg
     1141 ctatgttagc tgaaagcagt gtacaagaag tgatggattt agcacctgtt gcccatcttg
     1201 cttctattga aggagaagtc ccttttttga atttctttga tggattccgt acgagtcacg
     1261 agattcaaaa aatcgaagtt ttggaatatg aagaattagc tcctctgatc aatcaggaga
     1321 aattagcttc atttcgtaaa cgagggatga atcccaatca tccgactgtt agtggtacga
     1381 accagaatcc agacatccat tttcaacaac gggaaacgat caacccctat tatgataaat
     1441 tacctgaaat cgttcaaaaa tatatgaaag agatcaaccg attacgagga acttcttatg
     1501 acttggtgac ttattacgga gcagacgatg cggaagaagt cattgtttct atgggatcag
     1561 ttgcacagac gattgaacaa acgattgatt acttgaatgc aaacggacgg aaaacaggtt
     1621 ttttgaatat ccatctttat cgtccttttc cagtggaaac attcgtgcaa aagttgcctc
     1681 atacagtaaa aacaattgct gttcttgatc gaagtaaaga accaggggca ggtggagaac
     1741 ctctcttatt agacgttcaa agtgcgttat atgattctga acttcgcccg gctgttattg
     1801 gtggtcgcta cggattgggg tctaaagatg tgactcccga ccaaatctca gcagtttttg
     1861 atgaattgaa aaaagaccca agcatacgca aaaaacggtt taccataggg atcgtggatg
     1921 atgtgactta tcaatctttg gaaccaaagg aaagtctgga tttgacagaa ccgcaaacat
     1981 tccaggccaa gttttgggga tttggatctg acggaacagt tggagcaaat aaatcagcta
     2041 tcaagattat tggagatcac accgataaat atgcgcaagg ctatttttat tatgattcga
     2101 aaaaatctgg cggtctgact gtctctcatc ttcgttttgg agatacgccg atacgttcag
     2161 cgtatcttgt cgagcatgcg gaccttgttg cctgtcacac accagcttac ctgcatagct
     2221 atgacttggt caaaggattg aagccaggtg gcattttttt attgaatact ctttggagcg
     2281 acgaacaatt agaaacgcat ctgcccttaa aattaaaacg ttacttggct gaaaataaca
     2341 ttagattcta tacgatcaat gccatgagat tggcccaaga agtcggtttg ggacgccgga
     2401 tcaatacggc aatggaaacg gcttttttta aattagctga catcattcct tttgatgagg
     2461 tgcttccgct gctaaaagaa gaagccctga aaagctatgg acataaatca atggctatcg
     2521 tagaaaaaaa catacaagca atcgatcgga cgaccgaact gcttcatcaa gtcgcgatcc
     2581 cagaccattg gagaacgttg gagatgcctg aaaaaatcaa taaaaggaac actggttata
     2641 tacaagaaat acttgaacca gtgaatgcac aggaaggaaa tcaactctct gttggtacac
     2701 ttgtagcaaa tggaatgaca actggcgaaa tgcctttagg aacggcttct atggaaaagc
     2761 ggggcgtcgc tttagaagta cctgagtgga ttagtgatcg ctgtacgatg tgtaatgaat
     2821 gtgcctttgt ttgtcctcat gctgccatcc gtccattttt agcagatgaa gaggaaatgg
     2881 aagaagcgcc agaaggattt atcgtgagag aaatgcgagg cgcagacggc gtaaaatacc
     2941 gtatccaagt ttcagtggaa gactgtacag gttgtggtct gtgtgtagag gcttgtcccg
     3001 caaaagaaaa agctttagtg atgaagcctt atgaagaaca gaaagaacaa gctgtgaatt
     3061 gggcttttgc aatgacatta agacaaaaag caaatccagt caaaggaaag aatactgtct
     3121 tgtctacaca attcaatcaa ccgcttcttg aattctcggg cgcttgctca ggctgtggcg
     3181 aaacacctta tgtaaaatta ttgacacaaa tgttcggtga tcgaatgatg atcgcgaatg
     3241 cgacaggctg ttcttctatt tggggcggtg cttctcctgc ttctccttat acgaccaatg
     3301 aatgtggtca agggcctgca tggtcaaatt ctttattgga agataatgct gaatttggtt
     3361 atggtatgct tctagcggca cagactcgtc gcgaggcttt ggcaagttca atcaaagaag
     3421 tattgccaaa agtatcagaa gaactacgag aattgatgga agactggtta gtacacatgt
     3481 ttgagagtga aggaacgcag caaagagcag caaaactgca agctgcgatc gaaagcgaac
     3541 gatcaatttt ccctgaactg gaggatatat acaagagaaa ggatctattc gtaaaaaaca
     3601 gccaatggat gattggtgga gacggatggg cttacgacat tggttttggc gggatcgatc
     3661 atgtacttgc cagcggagca gacgtgaata tccttgtttt ggataacgaa gtctattcca
     3721 atactggcgg tcaaacctca aaagcaacac cagcttcggc aatcgctaaa ttttcggcta
     3781 gtggaaagta tgcttcaaag aaagatctag gaatgatggc gatgacatat ggaaatgttt
     3841 atgtggcgca aattgcttca ggtgccaatc aaatgcaaac gatcaaggca tttgaagaag
     3901 cagaaaagta tcctggtcct tctctgatta ttgcctatac gccttgtatc acacatggct
     3961 tgcttggcgg aatgaaagaa tcgcttaaag aagcaaaaga agctgtttct tctggttatt
     4021 ggtcattata tcgatataac cctacaatgg tcgaaaaaaa caagaaccca atgactttgg
     4081 actacaaaaa acctgatttc tctgctatgc ctgatttcat gaggaaacaa gttcgctttt
     4141 cctcgctaga taacgcaaat ccaaatcttg caaaagcttt atttgaaaag actgtccacg
     4201 acgctaaaac acgtttttac aactatgctg cactgacggg agaattagaa aaaatcaaga
     4261 aacgacttga accagaggaa tctatcgatg tacccgttaa gactagaaaa gaaaaaacga
     4321 cggaccttga agcagaagaa agaagagctg ctagaagagc tgccagagca gaacgacgta
     4381 aaaaaatgga acaagctaca aaagaaaaaa aggaaaaata acagaaaaaa tataatgaat
     4441 aattctctga agtttaaaaa tatttattgc agagaaaaat aaaacgcata cttgcaattt
     4501 tcttgaaaat tatgcgtttt tctcaagaaa aatacgagca tatcagcctc taaaattaaa
     4561 acatatgata tactagagag gaaatctcaa aagagaagag aggcttttta tgtcatttca
     4621 aatcggggaa ctttttaatt tggactattg gcagcaaatg ttttccggtg acttgttctc
     4681 tctgaatttt ttggtgaaca tacttgatat tctggttgtc tggtatcttg tatacaaact
     4741 gatccaactg ctgcgaggca caaaagcgat tcagcttctg aaaggtgtag ctatttttat
     4801 tgttatccga atcgtagcgg aaatcattgg tctgcacacc ttgtcttggc tgatgaacca
     4861 agtcattacg tacggggtga tagctgctat cgtgattttt cagccggaaa tccggagagg
     4921 actggaacat cttggacgca gttcgctttt caaacaatca aaaagcgaac agcaggaaga
     4981 tgagaagatg atcttgtctt ttgacaaagc catccaatat atgtccaagc gaaaaatcgg
     5041 tgcgttgatc actatcgaac gtcatactgg attagatgag tatattgaga cagggattgc
     5101 tttagatgca gatatcactg gggagctgct gatcaatatc tttattccta atacaccgct
     5161 gcatgacggt gcagttattg taaaagaagg aaagatcgca gtagcaagcg cctatcttcc
     5221 tctatcagaa agtatgttga tccctaaaga attcgggact cgacaccggg cagctgtcgg
     5281 tataagcgaa gtcagtgacg cgattaccat cgttgtctct gaagaaaccg gggacgtcag
     5341 catcacgtta gataatgaac tgatggctgg cttatcccag caagaatatt tggcaatctt
     5401 gcgtagagaa ctaattgctg aagagaaaac aaaaaacaag aaaaacgtga ttcaatcttt
     5461 cctagacggt atgactaagg gaggggatcg caaatgatct caaaagagaa acggacaagc
     5521 attctttaca gttttattgc attgctgttc agtattgcgc tgtacttcaa tgcaaatggc
     5581 cagtccgttc caaatacgct ttctggaaat gaagcgtata atcaaacagt tacgggggtg
     5641 ccgatcaaga tttcttatga ttcaaagcgc tactatatcc atggatatga aaatgcagtg
     5701 actgtcaaac tttccagtgc aaaccgagtt cagttgaatg cagaggcaaa cgaagataca
     5761 cggatgttcc gcgtcacagc agatatcact aagctgggcg aaggaacaca tgaagttccg
     5821 ctaaaaattc aaaatttgag cagtgctgtc acagcaaaga tcgaaccatc gactattaca
     5881 gtaacggttg agaaaaaggt aataaaagat ttcgatgtgg aaacgttgat tccttcaacg
     5941 ttcacaccat ctggatatga acttgatgat acttccgtta gtcctaaaaa agtctctatc
     6001 actacaggtg acaaaacatt ggcagaaatc aaacgagtag tagctgatgt ggacgcttcg
     6061 atggtcacag atgatgggat taatagtgag gtcccaatac aggcgctgaa tgcagcaggg
     6121 gaagtattaa gtatcgtctc tgatccagtg caagtaacag tgagagctga ttcgatcaag
     6181 ccaagcaagt cggtacgact ttatgggata cagcaaggaa caccagctgc cggggtagaa
     6241 agctatgatt tcagtttttc aaaactggaa gcagaagtaa gcggctctag cgatttgctt
     6301 gcatcgatcg gtgacagtat tgctgtaccg atcaatgtct caggtatcaa tcatcgaaca
     6361 actcgtaaaa ttgaaatacc agtagaagag gggttaagta tcaagcccga atcagttagt
     6421 gtggagatta cacccgtgtt gcaaacgtca agcagtagct ctgcttcgac gagcagtcgg
     6481 acacaagaaa atacaagtac gagtagaagt atttcatctg ctgattcagg aacggaacca
     6541 tcgacaacaa ccatttcttc cagttcggaa tctgggacaa gcagctcgac cacaagtaca
     6601 gaagaaacat cagaaacaag cgaatcaaca caaatgagca gaacaaattc ctcagaaaat
     6661 taagttaaag gagtaatttt acaactatgg gtaaatattt tggaacagac ggcgtacgcg
     6721 gtgaagccaa caaggaactt acgccagaac ttgcatttaa gctgggacgg tgcggaggtt
     6781 atgtcctaag ccagcatgaa gaagaaggaa aacgcccacg cgtgttagta gggcgggata
     6841 caagaatctc aggtcaaatg ttagaagaag cgctgatttc aggactttta tcggtgggta
     6901 tcgaagtgtt ccagctaggt gtgatctcta caccaggtgt tgcttatctg acacgtttgc
     6961 aaaaagcaag cgcaggggtc atgatttctg cttctcataa tccagcacaa gataatggaa
     7021 tcaaattttt cggtgcagat ggttttaaac tagtagatga ccaagagcta gaaattgaag
     7081 cattattaga tgctgaagtg gatgaattgc ctcgtccatc agctgaagga ttgggaactg
     7141 tagaagaatt tccagaaggc ttattgaaat attctcaatt ccttgtacaa acgatcaacg
     7201 gtgacctttc cggtttgacc atttgtgtcg atgcagcaaa cggagcaact tcaacaagtg
     7261 ttaatcgttt gtttgccgat cttgaaacag atttttatac aatgggcaca aatccagacg
     7321 ggttaaatat caatgacggc gtaggctcta cccatccaga acgtttagct gagatggtcg
     7381 ttgaaaaagg agcagatgct ggactagctt tcgatggcga tggcgatcgt attattgcgg
     7441 tggatgaact tggaaacatt gtcgacggtg ataaaatcat gtttatctgt gctaaatact
     7501 tggcagaaaa aaatcgattg aaaaaagata cgatcgttac gactgtcatg agtaacttag
     7561 gattccacaa agcggtggaa ggaatcggtt tgaaagatgt cgttacacaa gttggtgatc
     7621 gttacgttgt agaagaaatg cgcaaaaacg attataactt tggcggcgag cagtctgggc
     7681 acatgatttt cttagatttt aacacaactg gagatgggat gctttcagga attcagttac
     7741 tgaatatcat gaaacagaca ggtaagaaat tatcagagct tgcagcagag gtaacaatct
     7801 atccgcaaaa attagtgaat atccgcgtaa gtgataaaaa cggtgcgatg gatgttccgg
     7861 caatcaaaaa agtgattgaa gaggcagaag ctgaaatggg atcagaagga agaatcctag
     7921 ttcgtccttc cggtacagaa ccattacttc gagtgatggc agaagcgcct actgatgaga
     7981 aagttaatta ctatgtcgat aaaatcgcgg cagtagtcaa agaagaaatc ggactggatt
     8041 aagagatatt ttcataaaaa ataaaagaac aaggaccagt gtcagatgtc tgacactggt
     8101 ccttgttctt ttattattct actgtcactg atttcgctaa attacgtggt ttgtcaacgt
     8161 cgaatccccg taatagcgtt gcgtaatatg caagtaattg agtcggtacg actgtggtta
     8221 gagtagttag ataagggtgg acagttggta gcaccagctg gtcactttcc ttttctaatc
     8281 cctctgagac aataactagt gttttagcgc cacggctttc gacttctttc aggtttcctc
     8341 gagtatgcgc accagtcact tcttcactga tgattccgat cactggtgtt ccttcttcga
     8401 tcaaagcaat cgttccatgt ttcaattcgc cagcggcaaa accttctgcc tggatataac
     8461 tgatttcttt caatttcaaa gcagcttcaa gagaaacgtt gtagtcagca ccacgtccaa
     8521 tgtaaaaggc atttcttgta tcgctgagat atacttctgc caactcttct aaataatctt
     8581 tttgggcgat gaccgattcc atcgcattag caactaaact caattcatga gtgatatcga
     8641 atacaagaga agtttcaaat tcttttttat caccgatagc tttggctaat aaagttagta
     8701 cagctatttg tgcagtataa gctttcgttg atgctactgc gatttcgggt ccagcatgca
     8761 atagcagtgt aaaggatgct tcccgcgaaa gagtagaacc agcaacattt gtaatcgtaa
     8821 gtgaagggta gccgagacgg ttgattttta ccagcacttg gcggctgtct gctgtttcgc
     8881 cactttgact caagaaaata aagaatggtt tttttgaaag aagaggtgtg ttatagccaa
     8941 attcactggc taaatgaacc tcaacaggaa tctgtgtcaa tttttccaaa gtctgtttac
     9001 ctgctaaccc cgcatggtaa ctagttccgc aagcaacgat atagattcga tcgctatcag
     9061 acaatgtatc gaccagcttt tgatcaagag agacacgatt gtcttctgtt acgtattttt
     9121 gagcgatacg acgcatgata ataggctgtt cgtcgatttc tttcagcata taaaaaggat
     9181 aggtaccttt ctcgatatct gaagcatcta cttgtgcttc gaatgaagaa cgttctacga
     9241 tgtcacctgc aaaattttga ataataactt tttctgatgt gacggtaacc atctcttcat
     9301 cttcaatctc aacgaattcc ttcgtatgag caagcatagc cattgcatcg ctggcaatca
     9361 cattgaatcc atcgccgagt ccgattaata gtggactttt atttttagcg acgtaaataa
     9421 catcaggtgc tgtccgatca atcaaagcga aagcgtaaga acctttgatg acccgcaaag
     9481 cttttaaaaa cgcctcttgc gttgtagtat ccttcgcgaa agtttcaatt agatgagcga
     9541 taatctcagt atcggtctca ccaataaagt ggtcatttgc taaataagca attttaagtt
     9601 cttcaaagtt ttcgattacg ccgttgtgaa cgagcacgaa gcgtccactt tgggaagtat
     9661 gtggatgggc gttttcttca gaaggttgcc cgtgagttgc ccatcgtgta tgaccaatac
     9721 ctatatttcc tgtagtatca atatttattt tgtttttcaa attttgaatt ctgccttgtg
     9781 ctttgaccag ataatcagtt gtgccatctg agacaaagat accagctgaa tcataaccac
     9841 ggtattctaa tttttctaga ccgtcaatca atcctggtgt tacatttttt aatccaatca
     9901 ttccaacaat tccgcacata aaaaacatcc tactttctat aatgaattcg tatgaatgct
     9961 tccagctgca ggaaaatcct tttgttttat ctttgcagac attttttgtg gattttctgt
    10021 agagtgcagt ccctagggcc atccgccgaa tctttcgata aaccctctcc tcgtcatctg
    10081 tttacacaga cctggcgcta tcctaaaagg tataactatc ctcctttttg ttttttatct
    10141 ggaaacattt aaaatcataa ccttttgcga atgttcttgt caacaaaaaa agaataaaaa
    10201 ataaattggt atatatcaaa ttctttgtca aatgtccttt ttatgtcaaa aaataatggg
    10261 tttttatcta tcatcttttt caggagttcg gtacaataga aagtaatcgg atagaggagg
    10321 agtaaaaatg gattcaaaaa ttgagatcat gacactggga atgttaaaaa aacaattgtc
    10381 caaattcgaa gcatcagctg gtgtatcaga tgatacgaaa atatttttag atactggctg
    10441 ggatagcatc caagaaatcg cacctgatgc cttggaagtt gctcaagctc gtgagtttac
    10501 ggtagaagat gaattaacaa aagagtcgtt ttccgggtat gcgcgggaag aaaaagcaga
    10561 gcggttcgac acttctgagc agtcagaaac tgtgatcgtc ataaaaaact tgtattagaa
    10621 aggagaaaga atgaataaaa gacgttgggc agcagttctg attgctgcag gattatttgt
    10681 ggtttcgtta gtttcagcta gtttgactaa gcctcaggaa aacgatcaaa ttgagggatt
    10741 gaacagttgg ttatatgggg atgaagaact agctccaaat gtgttggaag agggagacag
    10801 tacgcaaaaa atcgtataat tatcagtaga tggaacgatt gctgcgggag ggtcttcagg
    10861 tttatttgct tctgaaggtt acaatcataa aaattttatg gaacaactga aggcgattga
    10921 agaagatcca acagtcaaag gtatcttttt agaagtaaat tctcctggtg gcggtgtcta
    10981 cgaaagtgcg gaaatcgcta agaaattgga tacgattcgt aaagaacatg atatcccaat
    11041 gtatgtcagc atgaaaaaca tggcagctag cggtggctac tatatttctg ctcaagcaga
    11101 taaaatcttt gcaacagaag aaacagtgac cggctcaatt ggtgtgatca tgtcaggatt
    11161 gaattattca ggacttctag aaaaactggg tgtggaagat acaacagtta aaagcggagc
    11221 gttgaaagat atgggttcag gaacgcgacc agagacaaaa gaagaagaag cggtgcttca
    11281 agcatatatc gacagcgcat atagtcgttt tgtagcaatc gtcagcgagg gacggaataa
    11341 atctgaagaa gaagtcaaaa aaattgctga tggaaggatt tatgatggtg tccaagcaaa
    11401 agaagtaggt ttagtcgatg aattgggatt tcctgaagat gctttaaaag ctatacgaaa
    11461 ggaacaacag cttgaagatg cggaactagt agaatattct tccacgccat ctgggtttgc
    11521 tagtacatgg ttaggaagta aactagcaga attccaggga ttgaagcctt ctgagactag
    11581 tcagatctta tccgttcttg agaatctagg gacagcggaa tcaccaaaag caatgtatta
    11641 ttacggaggt gagtaagcat gtcagagctt tcaaatgaaa ataaggaaca agaaaaagat
    11701 aagcaacagc agcctgctgt aggacctgac tccaactttt cccaaaaggt actgagctct
    11761 tttgaaagag agcctttgac gcccgaagaa atcaaaaaga aacaacgtaa atggaaaaag
    11821 tatacggaca caagaaatga accgccaaaa gcaaatgatt ttcctaagta tttctatgca
    11881 ggttttttta tccgaatgtt tgccttcttg gtcgatcttt tgtgtatcgg agctattacg
    11941 cgtttaacac ttggtattgc caaaaatcta ggttggctta atttttcaga ttcttatctg
    12001 agtctctacg gattcacagc cttacttatt tatctagcgt attttatttt attgacgaaa
    12061 ttcaatcatg gccaaacgat tgggaaaatg atctttggta ttcgagtcat cagctttgat
    12121 gaagaagaac ttagctggtc aaccgtttta gttagagaag gtgcttgccg gttcattcta
    12181 aaatttcctt tgctgatgtt agggtatctt ccagcagtct ttaatcagaa aaagcaacat
    12241 ctgggcgatt ttttcactaa tacaagtgtt gttacgatca atctgatcaa agcgttcaac
    12301 caggaagtca acagctgatg agcaataaaa agcagtaaca gttgaaagca ccttctaaaa
    12361 gtcagatcta aaatctagct tttagagggt gcttcagtta tttttaattg tcatgctttt
    12421 tttgttttgc tactattgaa catgagctga atatgagcaa gaggaggaca ctgcgtgaag
    12481 ccgattgatt ttccagaaaa atatgagaat ttaatgcgag tggcacaaca agcactagca
    12541 aaccagcaat atcagcaggc aaaagaatta tttcaacgag cttacgagtt gaaagaaagc
    12601 tttgaggcta atagcctgct tgttttttgt ctttacgagt tggacgagaa aaaagaagca
    12661 ttgaagcaag cactcttgca tgaaaaacag tatcttcaaa atgaggagtt tgctgaattt
    12721 tattttgact tgttaatcag tacacaagat tttttatatg ctagaaaatt gatagcaagt
    12781 acagattttt atgaagcttt cgagcagcgg gtcattgaaa aaatccaatt tgcagaagaa
    12841 ttatccggac aaatggagag gcagaagatc aaggcactcc acaataaatc ggaggaactt
    12901 ccttcgttgg aacctgcacg tcagcttgcg tctattgatg agatcgaaca gcttccttat
    12961 catgagttta tccaaacagc gagtaaactt attgtatcgc cagaggtcca tattttagca
    13021 agggcaaaat tactggagac acttaggcaa ttgaacgaat gccatcctgt tttctatttg
    13081 acgattgaag aaaagttagt caaagtcata ccaaaagatc tgccaaagcc gcagcagcag
    13141 gcaagttacc agcaattatg tgtattttca gatcgttacg ggaatgaaga tgcgctgctt
    13201 agttccgtgt taaaagaaga attcacgttg caatctgcaa ttgtttatcc tgtctatgat
    13261 acatacatcg aagacccgaa gcgctggttt caactgactg tagaggcgtg tacaggaaaa
    13321 acgatgtatg aaggtacaga agatgaaaaa caagattttt taaaaaaaag agaaaaaatt
    13381 ttgcagcaaa tgatcttctt ccattagatt ttatcagtga aaaagataaa aatgttatga
    13441 aaaacaagga ttctatccat tcaatatgtt tacaatttga aagggttagt gtactattta
    13501 aaggtatgca tgaaacgttt ttgatgtata aaatgaattt ctaaactcgg agggaattaa
    13561 atgactgcaa catttgaaaa aaaaggcacg aatgatggtg ttttaaaatt tacgattgcg
    13621 caagacgaaa tccaaaaagg attaacaact gcgtttaata aagtgaaaaa aagcttgaac
    13681 gtacctggat tccgtaaagg gaaagtttct cgtcaagtat tcaaccgtat gtacggtgaa
    13741 gaagctttat atgaagatgc attgaatgct gtattaccag aagcttatga agctgctgtc
    13801 aaagaagctg gcatcgatcc tgtttctcaa ccaaaaatcg atgttgaaag catggaaaaa
    13861 ggtcaagact gggtaatcac tgctgaagta acagtgaaac cagaagtaaa attaggacaa
    13921 tataaagatc tagttgttga aaaacaagac cgtgaagtaa cagatgctga cgtggatgca
    13981 cgtatcaaac gtgaacaaga agcacaagct gaattagtta tcaaagaaga tgctgcagct
    14041 gaaaatggcg atacagtagt aatcgac
//
LOCUS       DACEUZ010000072        14029 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.072, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000072 DACEUZ010000000
VERSION     DACEUZ010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14029
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.072"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(74..1030)
                     /locus_tag="IXG44_001735"
     CDS             complement(74..1030)
                     /locus_tag="IXG44_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HAQ1658457.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            1134..1736
                     /locus_tag="IXG44_001736"
     CDS             1134..1736
                     /locus_tag="IXG44_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ1658458.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(1824..3323)
                     /locus_tag="IXG44_001737"
     CDS             complement(1824..3323)
                     /locus_tag="IXG44_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HAQ1658459.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(3339..3863)
                     /locus_tag="IXG44_001738"
     CDS             complement(3339..3863)
                     /locus_tag="IXG44_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1658460.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            4161..4910
                     /locus_tag="IXG44_001739"
     CDS             4161..4910
                     /locus_tag="IXG44_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HAQ1658461.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            4901..5686
                     /locus_tag="IXG44_001740"
     CDS             4901..5686
                     /locus_tag="IXG44_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ1658462.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            5655..6371
                     /locus_tag="IXG44_001741"
     CDS             5655..6371
                     /locus_tag="IXG44_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAQ1658463.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(6473..7492)
                     /locus_tag="IXG44_001742"
     CDS             complement(6473..7492)
                     /locus_tag="IXG44_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HAQ1658464.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(7511..8569)
                     /locus_tag="IXG44_001743"
     CDS             complement(7511..8569)
                     /locus_tag="IXG44_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014372913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HAQ1658465.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
     gene            complement(8790..9938)
                     /gene="nagA"
                     /locus_tag="IXG44_001744"
     CDS             complement(8790..9938)
                     /gene="nagA"
                     /locus_tag="IXG44_001744"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HAQ1658466.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(10014..10850)
                     /locus_tag="IXG44_001745"
     CDS             complement(10014..10850)
                     /locus_tag="IXG44_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1658467.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(11034..12251)
                     /locus_tag="IXG44_001746"
     CDS             complement(11034..12251)
                     /locus_tag="IXG44_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ1658468.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            12634..12717
                     /locus_tag="IXG44_001747"
     tRNA            12634..12717
                     /locus_tag="IXG44_001747"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12668..12670,aa:Leu,seq:gag)
     gene            complement(12957..13955)
                     /locus_tag="IXG44_001748"
     CDS             complement(12957..13955)
                     /locus_tag="IXG44_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1658469.1"
                     /translation="MGETGWRATTLNYQWPVAFSLLTFYPFFQLLRGEEINRKIYWVS
                     IPLLIFLTNQEQVNACFFVLTSIVSLYLIVNGRYNYKLSVFSIISLAELIFSLTTPGN
                     ALRAAHEINKWFPEYKNFNFLNKLDLGISSFGKPFFLDMNILFLLLFFLIFLLTYRKC
                     QNYYVRILTALPFFLNLIIYFGNTMGQSFTYVNGNKRAMIWSSSNLNNLFTELGTKLS
                     LFYPGTWIATLVVLALLLCLIVGIYLSFDNKKTSIFLVILMIMGFCSRLIMGFSPTVW
                     ASGMRTYYILYVVIAILVLMAVKELMKSMSVQKNEFMQFGLTVLGICTFIITVINR"
BASE COUNT         4277 a         2845 c         2391 g         4516 t
ORIGIN      
        1 caggagaaca ccgtttaaag aagaggttgt gaaatcagct ttttgacctg aatattagct
       61 gaattcacaa cctctatttt tggatttttt gttgggagaa ttctgccttg atttcaataa
      121 aacttttaaa agactgcaaa aactggaaca attctgctcg atcctcgaat tcagggcgac
      181 tagtcggcag aggcatttgc cgatacaata cataaacttc ttcaatacgt gttaaaattt
      241 gtctgccatc atttgactct gaaaatgttt ctgcagtata aatcaataaa ccatagatat
      301 gttttccata ataagctggt tgctggattc tctctagatt ttccagcatg tctttaatca
      361 cattggcctg tgctcttctc atagagaagt aagtttcgta gtatagcggc tgtctgagcc
      421 agtagttttc ttgatgttct ctggcatctt cttggctttc gcgaatgaat gtcagtaaat
      481 gttcgcattt gatctgcaca tcttctttat tttctgataa aagaaattct gccatttgtc
      541 tcaaaatatt tcgaaattct ttttcgatct gtatttggtt ttccttcagc tgcttcgtac
      601 gatctggcat gtaaacattt gcaagcaaag caaatactaa tccaatcgcc attaatcccg
      661 cttcattgcc gatcagctgc caagacatgt tttcctccac taaataatga gtgaccaaaa
      721 ccgaatttac tactatccct tcggttaatt tgaagcgaga ggcaagcggg atgaacagga
      781 ataaaaaaag actgaagcca aaaatagtat acccaacagc aaaaaaactg aacagtgctg
      841 ttgccgtagc taatataaag gctgcgattc gattcatgcc cgtacgcaat gtagagcgct
      901 tggtatttcc tacacttaat aatgcaatga tccctgcagc tggccaatac aacagcgata
      961 agctacccgc tgtgaccatc gaaaagaaga cactgatcac tgttttgact gttcgcattc
     1021 ctatacgcat ttacttcccc cttttttatt ttaattatac ttgaataatg aaaaactagc
     1081 tatcagacaa aagtttaggt atgatagtaa aaagaccaaa aaggagaatc tctatgcaat
     1141 ttggccgttt tcgactaggt atgcgtacga tcaaaagtgc cttagctgtt tttttatgta
     1201 tcttgttttt tcatttgact caacgaggaa tcccgatgat tgctgcatta tctgctgttt
     1261 tttctttgcg tcaggacttg actacaaccg tttcttttgg aaagtctagg atcatcggca
     1321 atacattagg tggtacatta gctatcattt atttttttgt caaaagctat tttcacaatg
     1381 attttttggt agaattgatc ttgcttccta ttctagttat tattgtgatc gtcgtgtcag
     1441 atggtattaa caataattcc ggtatcattt ccgcaatagc tacgttgttg ctgattgcgt
     1501 tgagcatccc tcaaggcgaa tccgctttgt atgctatcca acgtgtacta gatactttta
     1561 tagggacatt cattgcaatc ggtatcaatt tctttcttcg tcctcctgaa acagaaaaga
     1621 aggaagaact atcagaagat ttggtcgaac tccaaaagaa agaacgattg ctaagaacaa
     1681 atttacaaga gatagaagct aagatccaaa aacaaaagaa aaatagcgag gactaatttt
     1741 cttttgcaat aaaataaaaa aatggagaga agaactttaa aaagtcttcc tccatttttg
     1801 attgaacggt gaagtgtact actttattct tccttcatcg agttttgttc tgcctctttt
     1861 ggcgcttcat acatatttac ttggctagta gtcccacgtg tcgacaaatt ctctacgatt
     1921 tccttcaagt caagtcctgt tgtcgctttt agtgtttctt gagtagaaga aagcaaattt
     1981 gtggcataat ttgtcactcg gttcgctcca ctggtttctc ctgaactgct tcctgtatcg
     2041 acaacagaga ttttttcgat atttcctagc ggctgtgctg cttcgcgcat caattgcggt
     2101 agcatatcga taaccatact caagacagct gcttcgccat attccttgaa ggcattggcg
     2161 atcttttgtt ttgcttcggc ttctgctttt ccttttgcta gtgctgcttc ggcttctgct
     2221 tgtccagcta aacgtgtctt attggcttcc gcttctgcca gtgcttccac acggaatcgt
     2281 tctgcttcgg cttcagtcac ttctttgact ttttgagctt gtgcttcttg ttctttagcg
     2341 taccgatcgg catctgcttt tttcttcact tccgaatcat attgcttttc acgacgtgtg
     2401 atttcttttt cttccagttc gatttgtttt tgacgttcta cgattttaat ctgcatttcc
     2461 tgttcgatta cttgttgttg tgctcgggca ctttctaaat tgtaggcttg gtctgctttt
     2521 gctttagcaa catcttgttc ttgtttgtag gttgctaatt tcagttcttt ttcttttaat
     2581 gattctgcga tctctgtttg tcgctgtaat tcagctgctt gtgattcttt ttctgcttga
     2641 gcttttttga tacgcgtttc ttttagagct tccgcttctg ctatatctgc atctcttttg
     2701 acttgggcga ttcttggttt ccctaaagag tcaagataac cgtttttgtc tctgacctct
     2761 ttgatagtga aagaaacgat gatcaacccc atttttgcaa ggtctacaga agcgacttct
     2821 tgtacgcttt gactgaactt atcgcgattt tgatagattt cttctacagt catagaacca
     2881 agaatcgagc gcaagtgtcc ttctaatact tctcttgctt cattttccag ttcttctctt
     2941 gtcttgccta aaaactgttc tgctgctgta gcaatctctt ctacagatga accgattttg
     3001 atgatcgaag taccatcaca catcaccggt acgccttgtt ccgtatacac ttcaggggtg
     3061 gagacgtcca atttactcga taatagactg atccgattcg aacgttgaaa aactggcaag
     3121 acgaatgcgc cacctccacg cacaattttg attttgttat tgctttcatc aatgtgcaca
     3181 tttttgcttc ctaaataact tccactgatg atcaacgctt catccggttt tgctgtctgg
     3241 tacttcgcta caaagacgat cagcaacatc agtaaaatca aagctgcgat tgcgatcgcg
     3301 ataaaaacaa ctggtatttc catttaacac acgctccttt aagcttttat catgctttca
     3361 taaggcatga cataacagat tctttccttg atctcaatga tcaatacttt cgttccccgc
     3421 tcagctgtct gttcttgggg gctgtaaaaa caagctggac gactaatgga accggtaaca
     3481 cttttcaact gaatttctcc catacctcgt acagggatcg gtgtgatcac aacagctact
     3541 tgtccttcca gagttttttc agaagacgac atcgttgatt cggcatgttt catcggcatc
     3601 aatatataga aattaagcaa aaataccaac actatcgata tgcctccact agcaataaat
     3661 aaaaataaag atgattgatc tgttaaacgc tcacctagaa aaccaaataa gcttgtgaaa
     3721 gttaaccaag ggataataag catcggatca agcgggccat caaaatcgaa tacatctcca
     3781 aaaataatca ataggactgc caatcctgca caaatcagca atgtatacca ataaatcgtc
     3841 tcaagtgcga ttccccctat catatacacg cctccttcca tttctacgtc tatcatacca
     3901 atgtttgaaa attctgtata gcttctttta ccgactcagt ggtgtttttt ataggaaggt
     3961 tgtgtttatc gttgacaatt tgttaaattt ttgttttcat gttaaaattt tcacaataaa
     4021 ttctttcgag gtagcctatg tttccttttt ttaagaaaaa accattaaag ctccagaaca
     4081 cgatcatcct tgtcgtttgt ttctccacat tcgtttcttt gattgtttct gccatattga
     4141 tcaggaattt tattctttcg atgaaccgat ctagtgtgtc agataaaatc tctagcattg
     4201 ctaaaatcgt tgccaatgat aaactcatcc aaaaaaagct gcagactggt gacccgacac
     4261 atgcgattca aggaataaca aatgaaatca tgattgcatc gaatttagat tttatcgttg
     4321 tcatggaccc tgaaggcatt cgatattctc acgcagaccc aagttacatc ggtcttcctt
     4381 tctcaagtca tgaagatgtg aaaaaagtga ttggaggaca agaatttttc tctgaaaaag
     4441 aaggaatgtt agggttcggc ttccgctatt tcgtccctat ttattatgat caagagatta
     4501 ttggtgcagt ttgtgttggc ttaacaaaca acacgattga caatcaagtc aaagatggac
     4561 aaaaaatgat tgttttttca ttgattattg gacttagcgt aggcatcata ggcgccgttt
     4621 ttcttgccca aaaaatgaaa aaagtactgc ttggacttga accacaagaa atagcagaac
     4681 agttaagtga aaaaacgatt atcgaaaatg aagtagatga aggaatctta gccatctctt
     4741 ctgataaaac tatcctcttg atcaatacac ctgcaaaaaa cattttctac caaacagagc
     4801 agctattgga tttcgaagta ggaaaaaaat cagtgataat ctatatgacg ttttgtttaa
     4861 agagacgatt gagcatcaaa cgaccgttcg gactcaatcc atgtatttaa atgggataga
     4921 aactatttct agcgttacac ctctttttag caatgaccgt tttttaggag cagtcgcaac
     4981 tttacgaaat cgatcagaag taatgaaatt aatggaggag cttgggggga cgaaacaata
     5041 tataaactcc atgagagctc aaacccatga attcatgaat aaacttcatg tcatttctgg
     5101 tttgattgat ttgaagaagt atgaagaagt cacccatttt atccagcagc taaatcaaaa
     5161 ttataaaaaa gagattggcc aagtctcaag tctgatcaaa gtacctgcaa ttgccggttt
     5221 tttattaggc aaaataaacg aagccaaaga acaatctgtt gaaatgctgt tagaaagcga
     5281 ttcttttatc cctaaaatgg agatggatga ctccatccat tcacttttgc aagtgttagg
     5341 gaacttactt gataatgcaa aagaagccgt gctacaaaaa catgaaagaa acggacaggt
     5401 ccggattcat ttaaactatg acacagaagc tgaagtattc atcgtgaccg tctgcgatga
     5461 cggtgtcggg atatcagaag aaatctcaga aaaaatattt acgctaggtt tttcgacgaa
     5521 gggaatcaat cgtgggtttg gattgaatct catccattca atcgtcaaac gccacggtgg
     5581 atttttagaa ttttccaaac aaacaaatgg cggtacaatc gtctatctag aattaccaaa
     5641 aaaggaagat caaaatgatg acaaatgtcc tgataatcga agatgatcca atggttgcag
     5701 aattgaatcg tacttttgtg gaaagcatcc ctaatatgaa cgtaatcgaa agtttcagaa
     5761 atgtagaaga aggaaaaaaa tatttcagac aaaaaaacgc agatctgctg ctgcttgatg
     5821 tatacatgcc tggcgaaagt ggtatccatt ttttatcttg gcttagacag caaggaaatg
     5881 atatcccagt gattttgatc actgctgcag atgatatcga aaccataaag aatgtgatcc
     5941 gctacggtgc cttagattat ttgatcaaac cgttcacttt gcaacgtttt cagctggcga
     6001 tcgaaaagta tcttgctttc tctcagacaa tccaaacgac tgaaaaagcc gatcagaaat
     6061 tgatcgatca agtattcaac cacaccactc ccattcacga aataccatca ttcatcgatt
     6121 tgcctaaagg tctgtcaaaa ctgactttaa aaaaaataag tgcatttatt ctaaaagaag
     6181 atactatttt ttcaacaaaa gaactggctc aaaaaagctc actgtcacgt atatccgtca
     6241 agaaatattt gaatttttta gtagaacaag atatattgaa cgaaagaatc aaatatttgg
     6301 aaatcggccg gccgctaacc tgttatgaat tgacaaagaa tgggcggaaa aagctcacag
     6361 attactgttg agtcatcgaa ttatcccata caaaatagtc caagtataca gaagtaacaa
     6421 ctgtttactt ggactatttt taaacatttt attgacgaat atccatgttt tcttatctta
     6481 attgacgggc tttaggaaga gatacctcat ttttcgtttt tccagtttct attatttcac
     6541 gaaagttctc gaaacttgtc gaaatcatat ttgataatgc ctcatctgta tttgaaccta
     6601 catgcggggt tacaagaact ctagggtaca tttctactaa ttgctggata gcagggtctg
     6661 gaatcgtctc ccatggcttg aaggatcgga aaaaaacctc tgtttcattt gcaaaaacat
     6721 cggttgcaaa accagctaaa tgattgcttt tcaaagctct taaaatagct tcattatctt
     6781 gaagctctcc acgggctgta ttaatcaaga tgctaccttt tttcatttga gctaaaaatc
     6841 cttcattgat catttgatcg ttttcacctg ggatatgagg tacatgaata ctgacaatat
     6901 cgcttttggc taataactct tcaagcgagt ggaacgtcaa tactttttta gcagcttctg
     6961 tctgatgaat atcgtagccg atcactttgg ctcctagtcc cttaaacaaa acagcttccg
     7021 tcaaccctat cctgcctgtt cctatgatcc ctactgtgca atttctgatt tcacgactaa
     7081 acatttgctc atccactcga aaatctttga aagatgttcg gatcgtcgta tacgcagtat
     7141 ttctcaaaag catcatggca aaagtcaaag ataactctgc tatcgcattt ggcgaatacg
     7201 atggtacacg ggctactgtc ataccaaaat cagctgccgc ttgtagatcg atatgattga
     7261 ctcccacagt ccgtgtaaaa acataacgaa tcccatattc ctgcattttt gcaagattgg
     7321 tctcatctgc tatacaattt cccctcaaca atacagcatc atggtccttt gctgacacga
     7381 tattgtcatg tgtcaacagt tcttcataaa gactgagatc ataatggtaa atattcaatt
     7441 tttcaaaaaa atctatctcg ttcggacgaa caccgtaaca tgcgattctc atactttctt
     7501 cctccatttc ttatgaaaat aaacctatgc catgaatcat ttgtaataca acgatccata
     7561 caggtgtcag taccacagca aaaatagttg acagtaagga tgcattggag gctaaaagtg
     7621 cctccctatc aaaactgata gcataggctg ctgccactgt tgcagctggt gtagccatca
     7681 tgataacgat tgttgccagt gcttcaaaat tgactggtag aatattcgtt gccgtcaaaa
     7741 ctgctagaat cacgatattc aatgcaggta caaataccac tttgactaga ctgtaatacc
     7801 atgaagtttt ttcagatgca gcttttttga agcttacttc acctaatgtt gcgccgattg
     7861 ctagccacgc caatggtgaa cttaaaccag ctaaataatc catcggctga aatagccacg
     7921 gtgcagtctg gtcaattcgc aaaaacgcta cttggcttat ctctccttct gcacctgtaa
     7981 ctgacacttg tggcagatac ccttggaaca gccaaatgaa aaggccggca aaagtagcga
     8041 taatgattgg attgacgaac atcgctttga tatttttagc ttccatcttc aagccactca
     8101 tttttatgta tccataagaa tacaaaaaga ttcggtagcc aatattgaaa attgaggaat
     8161 acatcacgcc gacaggacca taaatcgcac tgacgatagg cgttccgaaa aaggtcgtag
     8221 aaccaaagat cgtcaggaca cgcaacgttg tttgcttatc ccctttatag cttaagaaaa
     8281 aaggttttga aactagaata agaataatat aaataactaa tccccaaatc aagacattga
     8341 ttccttgcat cagtaattct gtattgatat cttgcatgaa agctcgaaat gccagtgctg
     8401 gcaaagccac tgttaagacg actttcgata gaatttttcc aacagattca gaaaaaatcc
     8461 ccttttttct acaaacaaac cctaataata taataaaaac tgttgaagtg attgcactga
     8521 caatcgacat atctgtcaaa gtcgatcgaa aaatgtcaaa taagttcatc tttctcctcc
     8581 taattatgtt catttattca caataaaaaa agcttttatt ttgctaatac gtattaacat
     8641 ttttaatttt atctttacat tgttacttat tgaacaatag gaaattaaag aaacttttga
     8701 aacttatgta aagtgatttt tactagaaca caaaaaaaac cttaaaacca aacttaagaa
     8761 gtttagtttt aaggttaact taaattatat tatgcgtgga aacgttccac gccatctaga
     8821 taagttgctt ccaatgtcat atcagggttc aatacgataa agtctgcatc gcgtccttga
     8881 gcgatttttc cacatgtatc atcgatttta cagctgattg ctggaatcag actagccatc
     8941 atgactgctt gttctggtgt tgctaatccc cagtctacaa catttttaat tgcttctttc
     9001 aatttcaata tacttccagc taagttgcca gattctagac gtgctgttcc ttcttttact
     9061 actactggaa attcgcctaa attgtaattt ccatcaggca tcccaccggc catcatacag
     9121 tctgtaatta atgcgacatg atcatgtcct gctttttcca ttaggattcc cgcagcattt
     9181 ggatgtacat gatggccatc acaaatcaat tcagaaaaga catgttctaa agataataac
     9241 gcacccacca tacctggttc gcgatgattc aatccacgca taccattgaa agcatggaca
     9301 aatacacttg cgcctgcttc aacagctttt tgcgcttctt ctagtgtagc attactgtgt
     9361 cctagagaaa caactactcc ttcttcagtc acttgtttga caaactcttc tacaccttga
     9421 cgttccggtg ctaaagcaat ttttttgatt aaaccacctg aagcttcttg ccattcatgg
     9481 aaagtatcca aatcaggatc cccgaaataa cttgggtttt gtgccccttt gtgttcttct
     9541 gtgaagaaag gaccttcaaa atagattcct ttgattttag ctcctggtgc ttcctgatac
     9601 attttgccga ttgtctcagc tacatctttt aaacgttctt tgcttgacgt caatgttgtt
     9661 ggtaaaaatg aagtcactcc acaagataac agaccttcag acatcacttt gatgccttct
     9721 gcatcattat ccattacatc atgattcata tagccgtgaa tatgtgtatc taccaaacca
     9781 ggagcaatcc atttgccgct ttggtcaatg atgttgactt ctccctcagg tagttctttg
     9841 gtgtattcac caaaaagtcc atcggtaatt tcaagatagc ctgcatgttt gacacctgac
     9901 gctaaaaaga atttgtcagc atagataaaa gttctcattc tattctctcc ctttgttttt
     9961 ctacctctaa agtactctta tttagagaag aagtcaagaa tggtctacac catttacaaa
    10021 ttttttgaaa tttgtttcac agcttgtgct aagccttcat agaatgctgc tctttctggt
    10081 tcttctttac gcttgtatat taagctttta agcacaaata aatcattgag gtaatagtag
    10141 ctattcttct cttgagccca atagattcct tgattcacct gttctaacgc ttcgtcgatt
    10201 tcattttgat ggaataagaa atttccataa ttataaaaaa tttgcgaaaa ttcacttttt
    10261 gatcgttcat cagctgattg atgcagcagt tggatgcttc gactcaaata gtagcgtgct
    10321 tcagccgttt ttcctgccac accatatatt tttccgatac aactaatcag ttgtatttct
    10381 gtgtcagaaa catggattct ttctttttgg ctagagtagc ttaatgcttc tttcaaataa
    10441 taaagggact gagagatatt ttgacgcaac gtgaattcgc aaacaccgag ataataataa
    10501 tagcgttgaa agtcagttgc ttgatacaga ttttcagaaa tatcagaaga ttttagtact
    10561 tgttctaact tccgataatc acgtttttgg aaacaactat ccaacagttc aaataattta
    10621 tcgtttgtcc gagtagtttt tcgagagaga ttcatcacat gatcaatcgt tacccctagc
    10681 cgttgacaaa tctgcatcat caccaataca ttcggcagtt cctcattatt ttcgatccga
    10741 cttaatacac tttgtgaaca aatatcatgc gaaagcattt tttgcgtcat tttttgttct
    10801 tttcgaatct ctttaatgat cgttccaaag gactcgatta acgtttccat aagtatcctc
    10861 cccaaaatat gcgtattcgc attctattcg tattttactt taggaagtcg caaacttcaa
    10921 gaaaagactt gcaactaaat aatatacttt agttatttaa gaaaactcca ctagaaagag
    10981 agcctcagtg tgtttgtctg tggccctcct actgaaaaat ttggttttta aaatcatttt
    11041 tggcgatagg ttgctaggaa ttctctcatt ttcttagctg tttgtttcaa atcatccatt
    11101 ttaggaagat aaacgattcg aaaatgatct ggctggttcc aattaaatcc accaccatgt
    11161 acaagtagaa tatgatgttc atgtaaaaag tccaaaacga atttttcatc atccaaaata
    11221 ttgaatcgct ttgtatcaat ttttgggaat atataaaaag cagcttttgg ttttacggct
    11281 gaaagaccag gaatatcatt gattgcatta tagatatatt ctctttgttc atagatcctt
    11341 ccgcctggca ggagcagctc atcgacactt tgataaccgc ctagcgcggt ttggataatt
    11401 tgctgagaca atacattgga acacaaacgc atggaggaca acatattcag cccttctata
    11461 tagcctttga cgtgtgactt atctccactt aggaccatcc agcctactcg aaatcctgct
    11521 acacgatgag atttggatag accgttcagc gtaacaacga acaaatcagg cgctaatgta
    11581 gcaatcggaa tatgttctag accatccatc actaagcgat catagatttc gtcggaataa
    11641 ataatcaggt tattttgtcg agctacttca acgatttctt ctaataattc ttttggataa
    11701 agtgccccag taggattatt aggattaatg ataacaatag cttttgtccg actcgttatt
    11761 tttgatttga tatcatcaat atcaggatac cattctgcct cttcatcgca aatataatga
    11821 acaggtgtgc ctccagctaa tgcaatagaa gccgtccaca acggatagtc cggcattgga
    11881 accagtactt catctccatt gtccagcaac ccctgcatac acatcgtgat caattcgctc
    11941 actccgttgc cggtgtagat atcattgatc gtcacattcg gaaaatgttt cagttggcaa
    12001 tattgctcaa ttgcttttct agcagaaaag atccccttgg aatctgagta gccttctgag
    12061 tcacgggcat tcatgatcaa atcacgaatg acttcgtttg gtgcttcaaa tccaaaaggt
    12121 gcgggatttc ccgtgttcaa ttttaaaata ctaatgccct cttcttgcat ccgctccgct
    12181 tcttctagaa cgggaccgcg aacatcgtaa ctgacacctt ctaatttgtt ggatttttca
    12241 aattctctca tcacttagcc aatccctcat ttcaaaaagt tagaactttc caaatttttt
    12301 gttatttact atttcaatat catactccat cctagaaaat tttgccactt atttaggaaa
    12361 aacgtaagta ttaaaaaaac acaaacaagc gttgagaact taattgtcat gatagtcatt
    12421 ccatagattc gctgcccaat ccattgcaat tccacaaatg attcctagca taaaaccaag
    12481 caaaaaacgt ccgaatgcaa gtccaatcaa tacacctaag atcattccca gtcctgaaaa
    12541 ttctccgtca tatttctcca tcttaataag tcctcccatt tttgagcttg acaactatgt
    12601 aaaacctcgc tatcattagt gtgttattat tacgcagatg tgttggaatt ggcagacaag
    12661 catgattgag ggtcatgtga gcgttagctc gtgtgggttc gagtcccatc atctgcacta
    12721 caactcgtga agttaatttt gcttcacgag tttttttgtt tgaacgtttc tttatgccat
    12781 tatatacata tcactgtatc ctttacaaaa agaacgtaca atcatgtaaa cagcctgagg
    12841 tgagtatatg aaggtcggaa acaagctact cgtagaaact tgcaagtgtt aaaacaatta
    12901 atcgaaaaac gagtaaaatc cctcacctaa tactaaagcg agggattttt tgtatattat
    12961 ctgtttatca ccgttattat aaaggtacaa ataccaagca cggttaatcc aaattgcata
    13021 aattcatttt tttgtacact catgcttttc attagctctt taactgccat tagtactaag
    13081 atggcgataa caacataaag tatatagtac gtccgcattc cggaagccca tactgtggga
    13141 gaaaatccca taattaacct tgaacaaaat cccattatca tcaaaataac taaaaaaata
    13201 gaagttttct tattgtcaaa acttaaataa attccaacaa ttaaacatag taataatgcc
    13261 aagactacta aagtagctat ccaagtacct ggataaaaaa gtgacagttt cgttcctaac
    13321 tctgtaaaga gattattcag gttactacta ctccaaatca ttgccctttt atttccattg
    13381 acgtaagtaa agctttgacc cattgtatta ccaaaataaa taatcaggtt taagaaaaac
    13441 ggtaatgcag tcaatatacg cacataatag ttttgacatt tcctatatgt tagaaggaaa
    13501 attaagaaaa atagcaataa aaataagata ttcatgtcta aaaaaaacgg ctttccaaat
    13561 gatgaaatac ctaaatctaa cttattaaga aagttgaaat ttttatattc aggaaaccat
    13621 ttattaattt catgtgcagc acgtaatgca ttccctggag ttgtcaaaga gaaaattaat
    13681 tctgctaagc ttattattga aaatactgat aacttataat tatatcgtcc attgacaatc
    13741 aaatataaac taactatact tgttagaaca aaaaaacagg cgttgacttg ttcttgatta
    13801 gttaaaaata tcaataaagg aatacttacc caatatattt ttctatttat ttcttctcct
    13861 cttaagagtt gaaaaaacgg ataaaaagtt agtaaactaa atgctactgg ccactgatag
    13921 ttcaaggtgg ttgctctcca accggtttcc cccattactg tcaatggtat taaacagtat
    13981 atagagcaaa ccaataataa atttttaaca tttattttct caaaaacat
//
LOCUS       DACEUZ010000073        13807 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.073, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000073 DACEUZ010000000
VERSION     DACEUZ010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13807
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.073"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(6..662)
                     /locus_tag="IXG44_001749"
     CDS             complement(6..662)
                     /locus_tag="IXG44_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAQ1658470.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            855..1133
                     /locus_tag="IXG44_001750"
     CDS             855..1133
                     /locus_tag="IXG44_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1658471.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            1152..2360
                     /locus_tag="IXG44_001751"
     CDS             1152..2360
                     /locus_tag="IXG44_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1658472.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(2508..2873)
                     /locus_tag="IXG44_001752"
     CDS             complement(2508..2873)
                     /locus_tag="IXG44_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HAQ1658473.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(3020..3793)
                     /locus_tag="IXG44_001753"
     CDS             complement(3020..3793)
                     /locus_tag="IXG44_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ1658474.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            complement(3967..4827)
                     /locus_tag="IXG44_001754"
     CDS             complement(3967..4827)
                     /locus_tag="IXG44_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HAQ1658475.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            complement(4827..6245)
                     /locus_tag="IXG44_001755"
     CDS             complement(4827..6245)
                     /locus_tag="IXG44_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1658476.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            complement(6302..6751)
                     /locus_tag="IXG44_001756"
     CDS             complement(6302..6751)
                     /locus_tag="IXG44_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ1658477.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            complement(7196..7459)
                     /locus_tag="IXG44_001757"
     CDS             complement(7196..7459)
                     /locus_tag="IXG44_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HAQ1658478.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            complement(7558..8853)
                     /locus_tag="IXG44_001758"
     CDS             complement(7558..8853)
                     /locus_tag="IXG44_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HAQ1658479.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            complement(8850..10121)
                     /locus_tag="IXG44_001759"
     CDS             complement(8850..10121)
                     /locus_tag="IXG44_001759"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAQ1658480.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            complement(10286..11155)
                     /locus_tag="IXG44_001760"
     CDS             complement(10286..11155)
                     /locus_tag="IXG44_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HAQ1658481.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            complement(11447..13057)
                     /locus_tag="IXG44_001761"
     CDS             complement(11447..13057)
                     /locus_tag="IXG44_001761"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HAQ1658482.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
BASE COUNT         3931 a         2911 c         2354 g         4611 t
ORIGIN      
        1 gttcatcaaa agataatatt gtcttctaac aagtccatat tcaaaataac gatatgatgg
       61 tctttaacgg tgataatacc atcttttttc agttgtttta aaattctgtt tacactgcta
      121 gcagttgaga tgccacaaaa tttggcgatt tcttcattcg tgatcgaaaa atcgatcagc
      181 atcccttctt ctgtccgcac accgaataaa tcatataatt cgtaaagttg cgtacaaaca
      241 gctcccagct ttccgtttaa tagcatttgc tgcatttttt tcatcgaata aagcagacga
      301 tagcggtaat actcttttac atagtgttgt agctttgggt cttggttgat attttgccaa
      361 aagcgcacac gattgattcg ataaaactct gcatattctg attcaactcg aatattatac
      421 ggcgaatcga tgaattgtga atattcgtct tttaaaagag agacgatttc aagatctttg
      481 atatagcgaa ggttaaattc tctcccatca tttgaaataa cacttgcttt taccgttccc
      541 tctttcaaga tatacgtatg ggaatcttcc atcccttcga atgtcaaata tttatgatat
      601 cctttgcgta taatcggaaa atcattttta tccaaatatt cctttaagac tctactatcc
      661 aatgatgctc ccccatccaa actttcatct aaaaagatca tacagtactt taaaactttt
      721 taaagtccaa aggtatttta ctaatgatat taacatttgt taagtacctt tttccatcac
      781 ttatgcgatt tttttccttt tttttattcg ttatactgca gaagtaaaat tttttaagaa
      841 tagagggaaa aaacatgaat tactggatga acacaatcat taatcgatta gaaacagcat
      901 atcaaacaag atttgatatg aaagcttctt tagtcttttt aaatgatgcg tatcaaaact
      961 cgatcgaatt gatcaaagca gtggatgaga atccaacaaa cgaatgcgaa gaatttttaa
     1021 atctgtttat gtctacaaga gatttattta ttcgccaact tgttgatcgc tatccatcca
     1081 attatcacga tgtagaagtt caaatacaaa aattaaaagc atattcagct taaggggaat
     1141 gtataacata catggaaaag aaattattta ataaatattt tattggaatc acattgatca
     1201 attttattgt ctacctgatt tattatctgt tgatggtcat catggcagtc attgcgcaag
     1261 acaccttaca cgccagcttg agccaagcag gttttgcttc tgggatctat atcatcggaa
     1321 cattgctggc acgcttattc atggggaaac agttagaact atttggtcgg aagtttacct
     1381 taagaggcgg cgcgatcttc tatctccttt caacattagc atatctttat acgccaacta
     1441 tcggggcatt gtatttagtt cgattcttga atggattcgg ctacggtaca gtttctacag
     1501 ctacaaatgc tatcgttact gcatatatcc ctgcatctaa aaaaggggaa ggcatcaatt
     1561 attatggatt aagtactagt ttagctgccg ctgttgggcc atttttagga atgattttgc
     1621 tgaatatgac tgattttcgt ttcattatct ggttttcaat tgtactggtc ttctttgtta
     1681 cgattgcctg ccttgtcttt cctgtaaaaa atattccatt atctgaagaa caaaaaatat
     1741 ctcttcgctc atggaatatc aatagtttta ttgaaaagaa agcacttttc attacaggaa
     1801 ttgccttttt gatgggtctt tcttattcaa gtgttttatc atttttatct tcttatacaa
     1861 aagtaatcca cttagttgct gctgggtcat ttttcttcgt cgtatatgct ttggtcatca
     1921 catttacacg tccattgact ggaaggattt tcgatgtcaa aggagaacaa tacgtgatgt
     1981 atcctagcta tttattctta acagcaggat tatttttact gagtgtaacg acgaatagtg
     2041 tgacattgct tatctctgga gcattagtcg gcctaggtta tggtacgttt atgtcaaatg
     2101 gacaggctgt ttgtttaaaa atcgtccgcg aacatcgaat cagtattgct ttatctacct
     2161 actttattgg tttagaccta ggcttaggcg ttggtcctta tttgatgggg atgctaaaat
     2221 ctcttacttc atttagagga ttgtatgtga ttgccggagt cattccattg atatgtacag
     2281 cactctatct tgttaatggc cgaaaacata gtcatgaagc agagggcgaa ttgattgaat
     2341 cattgaaaaa tgaaatttga tcttgatgaa tagataaaaa aaaccgttca gatctgaaaa
     2401 taatctgagc ggttttttga gtaaaaatag tttatgatta ttcttaatta aacatctatt
     2461 tatttttaaa gctttcattt atcgataaga atagtttaac tgttgattta ttccattttc
     2521 cgttcatctg gtacgatgcc ttgacttagc aaccacttgt ggaagaacca ttctgccaca
     2581 ccgatcaata gagcaggaat caatactttc atccaaatat cgactgtatc attcataaaa
     2641 tatagataga gccacaagat aactgcagct gaaccagcat ctgcgatcgt cgctgcgatg
     2701 ttccctactt tacgtaagat caacagatcc cctagcacat agatcatgac tacaataatt
     2761 accccaacaa tcgttgaatc cataaagcta acaccgtata aacctgtcag cacgacccac
     2821 agaatcgccc agatcatgat tgccttcact accaacgcct tcacatgttt cattttctgc
     2881 actcctttct cctggataaa aaagtaagat tatctaacaa gataaccttt tcattattat
     2941 accatcggct gagagaagcg agcaaagata aggtcctatc taaaaatgag gaagccactt
     3001 agaacattca cgtaataatt tatccgtaca gacattcgat atggtgttcc tttactttcg
     3061 catactgttc ttttgtcagt tctttacttg tcaccagata atcgatttca tgaaaagcaa
     3121 tggcttgatg catgaattta tgtgcaattt tttgctgtgt aactaaaacg gcaagccggt
     3181 ctgattgttt tctaaaagct tttttgacca ttgcctctcc ttcatgagtt tctgttactc
     3241 cttggctgaa atctagcccg cgacaactca ttatcgctaa atccgcataa tatgacaatg
     3301 catcgttata ggcttttgaa ccgttgattg ttgctcgttt cggaacaatt gcccctccca
     3361 atacagatac ttttgccgtt gtatattcac tcaacatagc agctgtgacg atgccattcg
     3421 taatgatctg caaaccatca aatcttgcta ggtttctgac taagtgtaaa gaggttgtac
     3481 tggaatccaa gaataagacc atatcatctc gaatcaaaga agaagctgta tctgcaatct
     3541 tacgtttttc tgtttcttct tctaatttgg tgatcttcat caggtaaggc acgtcaattt
     3601 ttcgattgtc aataagcatg gcgccaccgc gaactctacg gatcaatcca cttttttcca
     3661 gaaaaacaag gtcacgttta atcgtggctt cgctataatt tactaactcg atcaaatcgg
     3721 tgactcttgc aaattggttg ttttttagat aattcacaat ctcctgctgt ctattctcca
     3781 cttcattcaa cataaaatca ccccatttac tttatatagt aacaggaaaa aagaggttgt
     3841 gacagaagtg attggcttcg agaaataaga agaaattttt cgaaaaaact acttctgaac
     3901 atcgtgtgtg gttaagaggg tatgacaaga ctattgtcat accctcttga ccacacattt
     3961 ttatttttag tataaactag ccttgcctac tgtgttcaaa atttccattt tatgtttgac
     4021 cacttcttgt acctttttat tagctgctgg ataaattgca ttcggctcgt acatcgatgg
     4081 attttcactc aatatttctc tcaaagcatt gaaaaagacg cttttaagat ctgagctcaa
     4141 attcactttg gcaactccgt attttactgc ttcgctgact tctgcgtccg gattacctga
     4201 accgccatgt aaaacaaatg gaagatcgat ttgttggttc aattctttca gtaagtcgat
     4261 atttaatttt ggagttctgt ttttcggata caagccatgc gctgttccaa ttgctacagc
     4321 aagtgtatcg attcctgttt ctttcacaaa aatcttcgct tgatcaggat ctgtataaat
     4381 gattgtttca gcacctcctt cagcagaacc attattccca atcgttccaa gctcagcctc
     4441 cactgagaca tttaccgcat gagctaacgc aaccactttt ttcgtaatct gtacattgtc
     4501 gtcgaatgat ttcaaagacg catcgatcat cacggaagaa tagccgtttc gaatcgcccg
     4561 taagacatct tctatcgtac cgccatgatc caagtggata acgatcggca gtttacttgt
     4621 ataagcatag gctcttactg ccgcaacaaa ctcatcactt aaataggcga tttcatcagg
     4681 atgaatttct aagattactg gtgcttctaa tctctctgct tcttcgatga ttgctttcat
     4741 catatcatag ctgcaaatat tgaaggctgg tactgcaaat tgattctctt tagccacttt
     4801 caataactct ttcattgtat ataacattac gcttccacct catttgttag attttcattt
     4861 ttcgtccact tgtcttgttt tttctctttc actgtttttt tcaatagtcc aagcataaag
     4921 gctgtagtga ctgtacctga tgcaattgcc aagacataca taggtaaatt gttcataaca
     4981 tttggaatta gcatcaccca aatccctcca tgaggtgcca ttgaagtaac tcctactgcc
     5041 atggaaatcc ctgcagcgac tgctgaacct gtcatcaaac taggtatcac gcggatcggg
     5101 tcagataccg caaacggtat agcaccttct gtaatatacg aagcgcctaa aatccagcat
     5161 gattgacctg aagtccgttc ttctttggta aatttatgtt tgaataataa agtggctaaa
     5221 gcaataccta ggggaggtgt cattcctgca gccatacaag cagcaatcgg tgcatttact
     5281 ccagctgaca tcaaaccgat cgagaaagtc gaagtcgatt tattgatcgg cccacccata
     5341 tcagctgcca tcatgatccc gataagtaat ccgaacaata gcccggatgt atttcccaag
     5401 ctattcagcc aatttgtcaa agcatccaac acccatgtga tcggttgttc aatcacaaat
     5461 accatcaaaa gcccaataat taagatcgat aaaaagggga cgacaatcag ctgataaata
     5521 gcagaaaatt ctttttttac gctaatttta tttgccagaa aatcacagat ataccctgct
     5581 agcacgccac caatcattcc gccaagaaaa cctgaaccac ctgtagaggc cagtaatccg
     5641 gcaacaattc ctggggcaaa acctgctttt ccagcaatcg atgtagcaat ccccgcgcct
     5701 aacgcaggaa ccatcatggc aaacgctgta tctcctccga tccttgaaaa tgcagctgct
     5761 aacacattat agtctgctga gttgggatca gccgctgtaa tcccaaaggc aaaactcagt
     5821 gcgatgatga ttccgcctgc gatcacaaat ggaagcatgt aattcacacc attcatgaaa
     5881 tgattataga ttgtcggctt tttctcattt gcagctggtt ctttttcatt agacacaacg
     5941 aatacgccaa tatctctcaa tgcttcgtct attactactt gcggttcttt gattgctcgg
     6001 cttgtagaga cacgctttac ttttttcccg gcaaatcggt ccaattcgat ttctttatct
     6061 actgcaagaa tgattgcatc tgcctctttg atttcttgtt cagataactg attttctgtt
     6121 tttacacctt gtgtttcgaa ttttgcttga tggtttagat ttcttgctgt tacttctaat
     6181 ttttctgccg acatataagt atgagcaatt ccagtcgggc aagcagttac accaacgatt
     6241 ttcatgatta ttttcctcct ttttcttcac tatcgatact cggacaaaga ctctactatt
     6301 ttcattcttg actaatctgt ctgatgatat ctgctggcgt agtggcttct tttattccct
     6361 ttctaaaatc tgcattcatc aatcgtctgg agagtgtgct gagaatctct agatgcatat
     6421 ctgctgattt ttcaggaact acgatggcaa aaatatattc aattggcatt tcatctatcc
     6481 cgctatagtc cgttaatttg gctgtatgaa aaaaacacag tgctggtttt gtcacatatt
     6541 tgctttttgc atggggaatc ccaaatccat cttctattcc tgtggagatc tcctcctctc
     6601 gtttgaatag tgccttaacg actttttgaa catttcttac cttatttgtt tttttcaaca
     6661 catcccctat tcgctcaaat aatgatcctt ttgaatctat cgatggatcg aaaacaatca
     6721 gttcttctgc gatcaattga tcaaacctca taaaaaacct ctcccccaca aaaaatgtta
     6781 acgttttctt ggaactgagg tcagtatagc atcgctagct gtaccgttca atattatgaa
     6841 tgaacagagg aaaaagaccc atcatccgcg tgtataagtc attaaaatga acgatctagc
     6901 tcacaaacaa tcgcaagtct ataacaaaac aaaaagcctt ccttgaaagg aaagcttagc
     6961 ttctaatttt aggtaaatgg ttttttactt cgaaataacc cctcttcatt tcgttaatat
     7021 aaggtgtttt tatcaagtgg ataattaaaa ctatatacta gtttttcatt gttttgtgca
     7081 ttatgatcct acgacttttt cagcatcaaa cgaagtttta aattggcaaa aaacataaaa
     7141 aaacagacta tccctatcag atagtctgct aaacgttgag ttatcagcgt ttttattatt
     7201 ccgcagcgtt tgcgtctttg agaccgtatt ttttgttgaa acggtccaca cgtccgtctg
     7261 cttgtgtgaa tttttgacgt ccagtgtaga atggatgtga gtcagaagtt acttcgacac
     7321 ggatgactgg gtatgtatta ccgtcttccc attcaactgt ttcttgtgaa gtttttgttg
     7381 aacctgacaa gaatttgaaa ccagttgttg aatccatgaa tactactggg tggtaatctg
     7441 gatggatgtt ttctttcatg attttttcgc tcctttgccc tgctccattt gctgaaccag
     7501 agttgatttg tcgatttaca acattaacat cttaacatgt ttcagtcaag gttgcaatta
     7561 tcttttcaga tttctggtcg gatttttttt accaaaggag acatcttgaa attctttgaa
     7621 aaattgctga ttgttttttg ttttacgcaa aaagcggatc agttgttccg tatattctaa
     7681 tgaatctcca gtcatatttt ttcgaagttt ccaaatttct tctaattctt cacttggcat
     7741 caataattct tcttttcggg tacctgattt tttgatatcg atggctggga agatccgacg
     7801 ttcagataat tcacgtgaaa gttgtaattc tagatttcca gttcctttga attcttcata
     7861 gatcacgtca tccatacggc ttcctgtatc gactaaagcg gtcgctaaaa tcgtcaaact
     7921 gccaccttct tcgatgtttc ttgctgcccc gaaaaatttc ttaggtttat acaatgcggc
     7981 tgggtcaatc ccgccactca atgtacgtcc gcttggtgga acgactaagt tataggcccg
     8041 agcaagtcta gtaatactat ccatcaagat aacgacatct cgtttatctt cgactaaacg
     8101 cattgcgcgt tccaatacta attcagaaac tcgagtatgg ttttgcggct gcagatcaaa
     8161 tgtagaagat accacgtcgc ctttcacgct tcgttctaga tccgttactt cttccggtcg
     8221 ttcatcaatc agtagaacga ttaattctac ttccggatag ttttcggtga taccatttgc
     8281 gatttctttt aatacagaag ttttccctgc ttttggcggt gcgacgatca atccacgctg
     8341 accaaaaccg attggagaaa agatatcgat cattcgggta gatagtcttt gcggtgttgt
     8401 ttccagttga agctgtcttt gcggataaag aggtgttaag gctggaaaat gcggacgttc
     8461 ttttgcttct tccggatctt ttccattaac gctttccacg tgcatcaaac cataataacg
     8521 ttccgattct ttaggtggac gggcttttcc tgccactttg tccccatttc tcaaactgaa
     8581 tcgtctgatt tgtgaggcag aaatatagat atcttctgca cttggtccat aattgattgg
     8641 ccgtaaaaaa ccgtaaccat cttgaccaac gatatccaat attccttcca tataataaaa
     8701 tccttgtttc tctgcttgtg cacgaatcac agctagagat aattcctttt tattcatctt
     8761 actgtaataa ggaattttaa attctttggc ataactataa acttctttta acgttttgtt
     8821 ctctagttct gccattgtca aataatcgct cattcagctc ccacttcctc tatcatttca
     8881 attgatgccc ctaaggctgt tagtttctcg ataatatgat cataaccgcg taagatataa
     8941 tctacattgt aaatcgtcgt tgtcccttct gccaatagtc cagcaatcac taaacaagca
     9001 cctgcccgaa gatcagaagc cacgacttct gtgccgtgaa gtttatttgg accattcacg
     9061 atgatcatat ttccttccac ggtaatatca gcgcccattc gtgctagttc cggcacatgg
     9121 ttcacacgtt tcgcatagat cgtatcgatg atttctgaag tgcctgtagt catcaatagt
     9181 aacgcagtaa gtggctgctg cagatctgtg gcaaatcctg gataaggata ggtcatgatg
     9241 tttgcgggtt ttaattcttt cgaggggtaa acttcgattt catcttcgct gattttcata
     9301 tggacaccaa tttcctgcaa ttttgctata aagctttcca aatgttcaaa gatcacattt
     9361 ttcactttga taccatttcc tactgcagct gctaatgcta gataggttcc tgcttcgatg
     9421 cggtcaggaa tcatgaaatg ccgacagcca tgtaatgttt cgactccttc gatacggatc
     9481 acatctgtac ccgctcctcg aacttttgcc cccatattat tcagtaaagt tgccacatct
     9541 atgatttccg gttctcgtgc agcgttttca attactgtct gtccttttgc ttttacagca
     9601 gctaacatca cattgatcgt tgctccgact gaaaccacgt ccatgaaaat ccgattgcca
     9661 cgcaatcctt tattttctgt ccgtaaatac atggcaccat gttcatttgt cacttcagca
     9721 cctagagctt caaatccttt gacgtgtagg tcaatcggtc tagggcctaa ataacagcca
     9781 cctggcaggc ctactaccgc ttcgccaaat ttaccaagca gtgttcccat aaaataataa
     9841 gaagcacgca aactattgat ttttccgtgc ggcatcggaa tcgatacgac ttctcgagga
     9901 tcgatttcta gtatgttgtt ggaaaaatgg acagttgcgc ccatgatctc taaaatttca
     9961 attaatgaat gaacatcttg tatgtccggc acaccttcca agatcactgg ggaatcagct
    10021 aagattgcag ctggaatcaa tgcgactgca ctattttttg caccgctgat cgttacttca
    10081 ccagctaaag gtcggtttcc tttgattacg atttttttca tctgtttcca ccttgtttat
    10141 taatcttttt tcatggatat gcttgtattt tatcatagat aaaaaaaaga aacgagcatg
    10201 gtctgccgtt ttaagtaaaa aagaatggag agcgacttca aaagtcgttc tccattccta
    10261 tatatcgtat ctgttatgtg gatgcttacg ctttgttagc tgaaccgaac cagtcgatat
    10321 gagatttagc atcttcaacg attgctgctt tacctggagc taataattta cgagggtcga
    10381 atcctttacc ttctttatct ttaccagctt cgatatattt acgagtagct gcagcaaatg
    10441 ataattggaa ttcagtgtta acatttactt ttgagatacc cattgagata gctttttcga
    10501 tttgatcttg aggaatacct gatccgccat gtaatactaa aggcatgtct ggacctacag
    10561 catcagcaat tgcttgtaag tggtcaaaag ctaatccttt ccagttttct ggatattgac
    10621 catggatgtt accgataccg caagctaagt agtcgatacc tgtagcaacc atttgtttgc
    10681 attcttcaat atcagctaat tcgccgttac cgatgattcc gtcttcttct ccgccgattg
    10741 aaccaacttc acattcaact gagatacctt ttgcatgtgc tttttcaaca acgtctttag
    10801 ctaatttcaa gttttcttca aatggtagat gtgaaccatc gaacattact gaagtatagc
    10861 ctaattcgat acattccaaa gcagcttcgt aatcaccatg gtctaagtgg atagcaactg
    10921 gaacagtgat gttcattgct tcaactaaat cagtgattaa atctttacag attttgtaac
    10981 cacccatgta tttagctgca cccattgatg tttggataag tactggtgct tgttttgctt
    11041 cagccgcttc aaggattgct tgagtccatt ccaagttgtt tgtgttgaat ccgccgacag
    11101 cgtaaccgcc tttacgagca gcttttaaaa attcagctcc tgatactact ggcataataa
    11161 aattcctcct aagttttgta aaactaaatt tgttaagaca attcttaacc aacgacccta
    11221 ttttaccaaa catttcggta gttttctact aaaatcaatg aaaatgaaaa ttttcaagaa
    11281 gttttttgca aaaaataaaa tatccaatag cgctttaatc gaaaaaagtc ttttgctttc
    11341 tatctttttt cctacaaata ataacaaatc agaaaaagag aaaagtgaca agaaaattgc
    11401 ttatcccctt ctcttgctac ttttctcttt ctatcaagtt caaaaattat tttgtttcgt
    11461 gatctgctaa tgatgctccg acaaatgctt tgattaattt ttgcgggcga tttggacgag
    11521 aaatcaattc tgggtggaat tggcatccca caaagaattt tttctctggc agctctacga
    11581 tttctactaa acggttatct ggtgagacac ctgaaaagac tagcccttgt tcttcaaaca
    11641 attgacgata tttgttattg aactcatagc ggtgacggtg acgttcttgc acaacatcgg
    11701 ctccatcata tgcttcagcc gtcttcgttc cttttttgat tttacatggg tataatccaa
    11761 ggcgtaaagt tccacccaaa ttctcgatgt tttcctgatc agccattaaa tcaataatat
    11821 tgtttgctgt atctggattc gtttcagctg atgacgcatc ttctaatcca acgacgttac
    11881 gtgcaaattc gacacaagcc atctgcatac caagacaaat acctaaaaac ggaacgtcat
    11941 tttcgcgagc ataacgaatc gcttcgattt tcccttctac tccgcgatca ccaaatccac
    12001 caggaaccaa gattccatct gcgtctttca aacgttcttc tacattttct ctcgttaatt
    12061 cttgtgcttt gatccaatcg atctcgatgt ctgaattata ggcgaaacca gaatgtttca
    12121 atgcttcaac tactgagata tatgcatctg gtaactcgac atatttaccg acaagcgcaa
    12181 tccgtacttt tttcttcaag ttcaagacac gttcttctaa ttctttccat tctgtcatgt
    12241 ctgcttctgg tgtatctagt tttaaatggt cgcaaacaat ttgatccatt ccttgtgctt
    12301 gtaaaactaa tgggatggag taaagtgtat ctacatctgg cgactcgata actgcttctg
    12361 gttcgacatc acaaaattgt gccaatttgt ttttcacgct ttgagaaaca ggttgctctg
    12421 tacgtaccac caaaatattc ggttggatac ctaaaccacg tagctctttt acactgtgct
    12481 gcgttggttt agttttcatt tctccagcag ctttcaagta aggaatcaat gttgtatgga
    12541 tatacataac attatctgta ccgacatctg ctttcatttg acgaagtgct tcaaggaatg
    12601 gcaaagattc tatatcccct actgttcccc ctacttctgt aatgatcaca tctgaatcag
    12661 tcattttagc tgctcgcatg attttttctt tgatctcatt tgtaatatga ggaataactt
    12721 gaacagttgc tcccaagtat tcgcccttac gttctttacg caaaacttca gaatagattt
    12781 ttcctgtcgt cacgttggag tatttgttca aattgatgtc aataaaacgt tcgtagtggc
    12841 ccagatccaa atctgtttca gcaccatcat ctgttacgaa tacttctccg tgctgatatg
    12901 ggctcatcgt ccccggatcc acgttgatgt atggatcaaa tttttggata gttactttta
    12961 agccgcggtt tttcaataag cgtcctaaag aagctgcaac aatccctttt ccgattgatg
    13021 aaacgacacc gccggtcaca aaaatatatt ttgtcataag ttcaaaaact cctttttaat
    13081 ttatgtgcag gaaaagtctc agacaataaa caaagctccc tattcttcaa taagaatagg
    13141 gagccgaaat ttctgtaaac ttctgaccct ctaaaagggt gcccaaggtg tataatacaa
    13201 gctgactaag ggaaagtcaa gtactcaaat gaatactatc cgcttacaat tttttctttt
    13261 tcgatatcct ttgagaaatt atttcttaag tcctttatga gtgcaaaaaa aatttaaccc
    13321 acataataag cttgatataa acatagctaa cgttaaacct aagcaaatca gaaaatcgtt
    13381 actgaaaaat tttaagtgaa ataatagata aaaaaataaa acaaacatta tgatagataa
    13441 tatcccacct aacaaagaac tactttttcc tacttgcagc aacagtttat atagcttatc
    13501 gtagaaacaa tctttttctt tcacccttat ttacctcctt tacgtaaaaa acgtagtcca
    13561 cagttctcgg atttttggca tatcttttct attaaataat atcaaaaaca aaacaattcc
    13621 actaattttt tcaaagatga cctttttttc aacaaaagtg ccttgtaggg attcattgaa
    13681 ggtaacgaat cacataagta aaaaataagg agatgcgaca aaagtcgtgc ctccttattt
    13741 ctaaataaac ggtgttctcc tgccaactcc tcggtcacaa gtcacaatat ccatcaaaca
    13801 tgagaag
//
LOCUS       DACEUZ010000074        13807 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.074, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000074 DACEUZ010000000
VERSION     DACEUZ010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13807
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.074"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(161..2158)
                     /gene="tkt"
                     /locus_tag="IXG44_001762"
     CDS             complement(161..2158)
                     /gene="tkt"
                     /locus_tag="IXG44_001762"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAQ1658483.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2260..2511)
                     /locus_tag="IXG44_001763"
     CDS             complement(2260..2511)
                     /locus_tag="IXG44_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HAQ1658484.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2658..3281
                     /gene="lexA"
                     /locus_tag="IXG44_001764"
     CDS             2658..3281
                     /gene="lexA"
                     /locus_tag="IXG44_001764"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HAQ1658485.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3461..3679
                     /locus_tag="IXG44_001765"
     CDS             3461..3679
                     /locus_tag="IXG44_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1658486.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(3968..4480)
                     /locus_tag="IXG44_001766"
     CDS             complement(3968..4480)
                     /locus_tag="IXG44_001766"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HAQ1658487.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4494..6791)
                     /gene="recJ"
                     /locus_tag="IXG44_001767"
     CDS             complement(4494..6791)
                     /gene="recJ"
                     /locus_tag="IXG44_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HAQ1658488.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(6933..7367)
                     /locus_tag="IXG44_001768"
     CDS             complement(6933..7367)
                     /locus_tag="IXG44_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HAQ1658489.1"
                     /translation="MKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPLILV
                     IIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQREFRN
                     ERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7377..8165)
                     /locus_tag="IXG44_001769"
     CDS             complement(7377..8165)
                     /locus_tag="IXG44_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ1658490.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8162..9103)
                     /locus_tag="IXG44_001770"
     CDS             complement(8162..9103)
                     /locus_tag="IXG44_001770"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HAQ1658491.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9280..9867)
                     /locus_tag="IXG44_001771"
     CDS             complement(9280..9867)
                     /locus_tag="IXG44_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HAQ1658492.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10088..11401)
                     /gene="obgE"
                     /locus_tag="IXG44_001772"
     CDS             complement(10088..11401)
                     /gene="obgE"
                     /locus_tag="IXG44_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HAQ1658493.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11689..12699
                     /gene="gap"
                     /locus_tag="IXG44_001773"
     CDS             11689..12699
                     /gene="gap"
                     /locus_tag="IXG44_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAQ1658494.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12784..13719)
                     /locus_tag="IXG44_001774"
     CDS             complement(12784..13719)
                     /locus_tag="IXG44_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ1658495.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         3807 a         3095 c         2436 g         4469 t
ORIGIN      
        1 tcaagaaaca caaactaatc cgaagaattc tttgttttgt catttaaatg taacatagcc
       61 gaaaacgaaa agtaatattg atttattctt ctaacacata aagaacagct gtctccaact
      121 tgcagacagc tgttcttatc agtcaaacaa ttgcttggat ctatgatagt tgattgtacg
      181 tattcacgac attttctact gtaaatccaa attcttctaa aattttagtg ccaggggcag
      241 atgcaccgaa atgatcgatt gtaatcgttg cgccctctgt tcctacgtag cgttcccatc
      301 caaatgatgc agcagcttcg atagccacac gtttcttcac tgatttagga agtacacttt
      361 ctttatattc agaagattgt ttttcaaaga ggtcaaagct tggcatagaa acaacagaaa
      421 catctttgcc tttttcagcc aattcttttt gagctttcac agccaaatca acttcagaac
      481 ctgttgcaat caaaatacct tctggtgttt ctccttgtga tggagaaaga acatacgcgc
      541 cttttttcac catttcatct gctacttctt ttgtagaagg caatactgga aggttttgac
      601 gactcaatac aaggatagta ggcgcatctg ttgtctccat cgctactttc caagcagcac
      661 gtgtttcatt tccgtctgcc ggacgaatca cttgaacacc aggcatgcag cgaacagatg
      721 ctagttgttc aatcggttcg tgcgttggtc cgtcttctcc gacagcaacc gagtcatgtg
      781 ttaagacgta aacaacagga gtatgttgga ttgctgctaa acgaactgct ggtcttagat
      841 aatcgacaaa gacaaagaat gttccaccgt agatacgagt tccgccatga agttggatac
      901 cattcatagc cgaagccatt gcaaattcgc ggacaccgaa ccagatgttg cgtccttcat
      961 aatgttcagg agtgaaatct ttatctgcag ccaccattgt attattggaa cctgacaagt
     1021 ccgctgaacc accccagaag cttggtatag ctttcgataa ttcttggatg acttctttac
     1081 ttgaaacacg gcttgcttga ctgcttccaa cttcataagt tggcaattct gcatcccagt
     1141 tttctggtag tttgccatca aaagcatctt caaattgttg tgctaattca ggataagctt
     1201 ttttataatt ggcaaacatt tcattccatg catcttcagc tttttgtcct tcttctatca
     1261 tcgtttgatg gaagcgggca gctacttctt ctggaacagt gaaatctgga tactcccaac
     1321 cataaacttc tttagccatc ttgatgccat cttcaccaag aggagcacca tgaacagcag
     1381 atgttccttc tttaggagca ccatagccga tgactgtttt tacttcgatc aatgttggtt
     1441 tgtctgtttc agcttttgct tcttcaatcg ctttagagat tgcttctagg tcatttccgt
     1501 ctttcactaa gatatgttgc cagccatatg cttcatagcg agcaccaacg ttttctgtga
     1561 aagcttttga agttggaccg tctagtgaaa tatcgtttga atcgtataaa acgatcaatt
     1621 tccctagttt catatgacct gccatcgaac ttgcttcttg agatacccct tccatcaagt
     1681 ctccgtcacc gcatattgca tacgtatagt gatccatcac attaaagttt tctttgttat
     1741 acgttgctgc aaggtgcgct tctgccattg ccataccgac agccattgca atcccttgtc
     1801 ctaatggccc tgtagtggct tctacaccat ctgtatgatg tacttcagga tgtcctggtg
     1861 ttttagaatc ccattgacgg aattgtttca aatcatcgat tgtcacttga tagccagcta
     1921 aatgaagcaa gctatacaac attgctgacc catgtcctgc cgaaagaacg aaacggtcgc
     1981 ggtcagccca gttttttgat gtcgttggat ttactttcag gtgttttgtc cataacgcat
     2041 atgccatagg tgctgcgccc atcggcaatc caggatgtcc tgagtttgct ttttggactg
     2101 cttcaatact taatgtacga attgtgttaa cgccaagttg gtcaattttg tcaaacaaaa
     2161 taacccctcc tattaattta ggtttttttt acctttattt cttacacttt cattctataa
     2221 gatgtcacac gaaaaagcaa tctttttcag tattttttac taaaagttgt catttcggtt
     2281 gtgtaagcct tttttctttt ggatttcttt taatttttcc ggggtcacgt cattgccctc
     2341 aggatcgacg actttcattc cttcgatatg atgacgcata ccggaacgaa aagcttccaa
     2401 atactctttg cgcaagttga tttgttcttg ttcttcggct tttgagagac cattttcctt
     2461 ctttttacgt gcaagttcat tgatccgctc tattttttca ggtgacagca tgctcagccc
     2521 tccttttact ctccttcata ttatataaaa actgaaaaaa aaacaactag ataaactaaa
     2581 taaaatacga acattcgttt gatttagcgc tttttatatg gtactattta tttatcaagt
     2641 gaaagaaggt gtaccttgtg gcccgacaaa ctgaatcaag acaaatcgaa gtcttacgat
     2701 atatccatga acaagtaacc caaaaaggat acccgccgac tgtccgtgaa atcggggaag
     2761 ctgttcagct ttcttctact tctactgttc acggtcatct tgcacggctt gaaaaaaaag
     2821 ggtttatcca aagagatccg acgaaacctc gtgcaatcga gttgacaaaa caaggtttag
     2881 aaaagattgg gattcgtcca acaactattc cagtacttgg cgtcgtaaca gctggagaac
     2941 ccattttagc agtagaggat gcttcagact tctttccaat ccctcctaat ctttcttccg
     3001 aagaaggcag tctctttatg ctgacaattc gtggagatag tatgatcaat gctgggatat
     3061 tggatggtga caacgtgatc gttcggaagc aagaaactgc tcaaaatggc gatattgtca
     3121 ttgcaatgac tgccgaggac gaagcgactt gcaaacgatt ctataaagaa aaaaactttt
     3181 tccgtctgca gcctgaaaac gacttgctcg aaccaatcat cttagatcaa gtatctatat
     3241 taggacgagt agtaggactt tatcgtagtc atatctatta attgctacta attaaaagaa
     3301 aactttttaa aaagagtttg agacgttggt ctcaaactct tttttcgccc gggttcttct
     3361 tctttcaaat ttttatcact tccctcttta caaaccgaac aaatattcgt atactaaata
     3421 tacgaacaat tgttcgcata aattaaagag gtgagaattc atgaaatcaa tgagacgtct
     3481 tggcttagta ttaactgttt tgtttttagg tattattatt ggtaatttcg gcatacgtcc
     3541 agctttactt gtgtttgttc cgttatttac tttgtggttc atgttgtggg atgaaaagaa
     3601 gtaccgtcaa tctgagcggc gcagaattca tgaagatcat ttctatcatg agccctacta
     3661 cagatccagg caatcataat aaggattgac tgctaattat tcaaaagaca agaagttaaa
     3721 cttttacggg ggtgtgacaa aaatcggtca catccttttt ttcgaataaa cggtgttcta
     3781 aagccagttt ttcggtatta agtcaaattg cggaaaaagt gattttttaa acttctactg
     3841 gtctttgctt gtttccattt ataaaaaaat agcgaccctc aaaagtcaga tagagaatct
     3901 gacttttgag ggtcgcttta aaatcgcttc tttaagaata acatcaagaa tagcttcttt
     3961 tttctgctta atattccatt aatgtaatga tgtcatatcc tttgattttg tcgcgtccat
     4021 ggagatccat caattcgatc aaaaatgcgc agcctacgac tacaccgcct aattgttcga
     4081 tcagttcgat cgtcgctttg attgttcctc cagtagctag caaatcgtca caaatcagca
     4141 cacgttgacc tggtttaatg gcatccttat gcaatgtcaa tgtatctgtt ccatattcta
     4201 atccataagt cacttcgatg gtttctcgag gtaacttccc ttttttgcgg actggggcaa
     4261 aaccgactcc taactcataa gcaacaggac atcctacgat gaatccacgg gcttctggcc
     4321 caactaccat atcaattcgt ttttcttttg cgtaatttac gatttgttta gtagcttccc
     4381 gataagcgtc accatctgcc atcagtgggg aaatatctcg aaacacaatt ccttttgacg
     4441 gatagtcggg aatactggca atataatctt ttaaatccac tctttgtttc ctcctaggtt
     4501 gttaaccatt tctttatggt aggaatatcg ctcaaaagca aaaattcctc aactttgatt
     4561 ttattgagcc gtcgctggta taatctgctc tcagtcaaag gatgactttc tgggctagcg
     4621 actttttgta aaacaccgtt ctctattgta acaaatttca attcaaaaaa cacctgtatc
     4681 ataaaaatta ataatttttc agggatacgc aaatattgag ctattgcttt tattttatga
     4741 cgtatatcaa ttcgttcttg agaatggatc aatttaaaca attttgcgta ctgttctctt
     4801 gtgcctaccc cattcaaata ggcctcatca ggtgaaatcc ccatcagata aacacgattg
     4861 aatgaataag accgaaaaac ttctttcaga tccgctaaat catctggaca atccataaca
     4921 actagagagt ttggttttct tttttcaatt attgccttta cagaatctag tccttcgaaa
     4981 acaatcgcag cttcctggat aatctcaggc acgaatttca acgtcgaaga atcaaatgct
     5041 aaataaaggc taccttccga cgttcggctc tgttcacgga aacgttttgc ccgccaatca
     5101 aatacttgca gaccacccac agaaaaatca gataccatca gctgcggttt cttacgcccg
     5161 ttccattcat tgatcgaaag ctttcctacc acatctacct gattattcag cagttcttct
     5221 tcttgagtac caaaaccaaa agcgaccgca tcaagctgac tcgatgcatc ggaaaggaca
     5281 aatttcagat gctgttggtt tgctccgatt cttttgatat tttctgcttg gacttgtcga
     5341 aataaaaact gaggcaaagg attatccgta ccaaatggcg ccaatagttt cagctcatca
     5401 attcgctcta ctgttacttc atttggcagc aatacttcat caattctcaa ctcaggccct
     5461 ctcatcaagt caatttgatg atcgactata tattggttca tcttctcctg tagtatagtg
     5521 acattttcac ttggcatcgt taaaccgact gctgcatgat gtcctccgaa gtaagtaaat
     5581 aaatctcgca tctggtcaag catttcaaaa agattcaagg cttcaatact acgtccagat
     5641 cctttagctg atccatcttc ttttaaagta aggacaagcg ttggtttacc cgtttcattc
     5701 attattttcc cagcaacaat gcccaaaacg ccttcatgcc atcctcggtt taccaataga
     5761 tggatatgat tctccttatt gaccatcgct aatgcttctt gtgttatctg ctctacgatt
     5821 gcttttcttt cttcattgat ctcattcaat ttttttgctt gaacggatgc ttcctcttca
     5881 tcaaacgtcg caagtaacga gacggcagga cgtggatcgc ccatccgacc gattgcattt
     5941 agtctgggag caatcgaaaa accgatcgtt gtttcgtctg cgtccctcat ttttacgccg
     6001 ctttcttcaa atagcgcctg aagtcctatc cgttctgaat gatgaatggc atcgattcct
     6061 aatgaaacaa ggattcgatt ctcatcagtc aatgaaacta aatcggcgat cgttcctatc
     6121 gctactaaat ccaaaaattc tgcaggcggt tcttccaata atgcagaagc tactttgaat
     6181 gctactccga ctcctgccaa atctggaaat ggatagtgcc ccgctggatg acgagggtga
     6241 acaatcgcat aagcgtctgg taaattctca ggtagttcgt gatgatctgt aatgatcacg
     6301 tctacaccag cttgctgagc gtatgctacc gcatcatttc ctgctacccc gttatcaaca
     6361 gtgatgatca actggattcc ttcttcgatc ttttcttggt ataccgattg gtttggtcca
     6421 tacccatgct caaatcgatt tggcaggaat acttctactt ctgcaccaag taattccagt
     6481 gtttctttca ttaccgttgc actggtgatc ccatctgcgt cataatcacc gtaaacgagg
     6541 attttttctc cactgattac agcttcttgg attcggctga ttgctttatc catatcgtgt
     6601 atcagatacg gatcatgtaa ttcttgttcc tttggatgta aaaatcggtt gatatccttt
     6661 tcctcatggt aacctctttg ccacagtaac ttaccgacaa atggagataa tttcagttca
     6721 cggatcattt ggagaaagga ttcagaaggc tctgtctcgc ttaacttcca atcaaaattt
     6781 gcttgtctca ctttctcact cctaacttag ttattatagc aaaggaaatc agcaaaaaaa
     6841 agcagaaaga aagaataaaa acagatcaga tccgctagac agatgttcag caggacctga
     6901 tcttttgtac gaccatttat gaaatatccc tattagtcaa aataattgaa gctttgtttg
     6961 ggcgattgag ttttttgttc ttccggcgct ttcaattgac tgatttgtgc ggttttcgct
     7021 tgtaactccg cctgataagc agcttccaac tccgcacgtt cgtttctaaa ctcgcgttgc
     7081 tgttcttctt ttgcttcttc aattttcttt tgaagttctg tttgatattc tgacatttcc
     7141 tgagtcagct gtttgatttg ctttttttgc tgccatagtg ttgccgaagc agtcaaaaat
     7201 acaatcagtg caccaatcag cgcagaaccg atgatcacta agatcaatgg cgcagaaaag
     7261 gaagtgaagc caaaacttac aggaacattt ttattgttca atacagcaaa aagaacaata
     7321 atcaaaacta aaacaaatcc aagaatcaca ttgctttgct ttttcaattc cttcacctac
     7381 tttttgttaa aaattccgct ggctaaataa tcaccaatcg ttggaaacag cgtataaaat
     7441 tttgatgcaa cttctaaaat aaacggttga ttgatttccc gttttggatg tctcatattg
     7501 cggacgatca tttttgccaa cttggcaggt tctaaaacga tttgtccgac tttttccaaa
     7561 tagcttccgc ttggatcagc cttatcgaag aaatttgtct cgattggacc tggattcact
     7621 gtcgtaacag ctacgccaag tggtttcaat tctaatcgca aagcgttgga gaagccaagg
     7681 actgcaaatt tcgtggcaga ataaaccgtt gattttgcag ttgccatttt tccagccata
     7741 gaggcgatat taatgatatg accttgtctt ctttctgcca tatcgatcgc aaatttttgt
     7801 gtcaaaacca tcattcctaa gatgttgact tcaaacatat cgtatgcttt tccaaggtcg
     7861 aatgtaagga agtcttcgaa aatcccaaaa cccgcattgt ttaccagaat atcgacgcgc
     7921 ccgacttctt ccatcacctt ttcatataat tcgtccacgc tttcaggatt gctgacgtct
     7981 accttgaaag cataagcctc tttgccgctc aagcgtctgc actcgtctct gacaccttct
     8041 acaaatgcca ttcttcgtgc gcaacttata acgattgctc cttgctttgc tgcttcataa
     8101 cagatttgtg cgcccagacc gcctgaacta cctgttacta aaacaacttt atcttctaaa
     8161 ttcatgcctc ttcctcctcg cgaaatggaa tctcaatgct atcaaaatct ttcataatcg
     8221 tcgtgttttc aaacacttct tgtgcttctt tttcaagctc taaagctgct tttcctaaat
     8281 atctcgcact gatatgagta agtagcagcc gttttgcctg cgcttctttt gcaacttcag
     8341 cagcttgatg agtcgtggaa tggaaatagt tgtgcgccat tcttgcttca tctttattaa
     8401 atgtgctttc atgtaccaga atatcgctat tttccgcaag cacaacggaa ttatgtgttt
     8461 ttctcgtatc gcctaaaata gtcacgattc gtcctttttt atctggacct acgaaatctt
     8521 ttccattgat cgtccgccca tcttctagtt ggatcgtttc accttgcttc agtttgccgt
     8581 acagtggtcc agctggaata cctaattctt ttagacgatc cacctgcaat tctcctttat
     8641 gatcatgttc aaccacacga taaccaaaac tgtcgatccc atgatttagt ttttttgtat
     8701 aaacagaaaa ttgctgatca gtaaaaatcg gggctgtttc atttagctca atgaatttca
     8761 gcggataact taaacgcgtt tgcgaaatac ctaaagagac ttttatgtat tcctctatcc
     8821 cttttggccc gtaaatctct aatggcgtat ccccaccttg aaatgaccgg ctgctgatga
     8881 gcccgggaag tccaaaaata tgatcgccgt gtaaatgtgt aatgaaaatt ttaccgattt
     8941 ttctcggacg aatagtcgtt cgcaaaatct gcatttgcgt gccttcgcca cagtcgaaca
     9001 accaaacttc attgcgctcg tctaaaagtt tcaatgctat acttgtcaca tttctatgtt
     9061 ttgcaggaac acctgcacct gttcctaaaa attgtaattc cataatctac ctgactttct
     9121 ttaagttcct ctttaatttt agggtaaaaa gaagggataa tcaaccgtaa gtcgagaatg
     9181 ggttcaaatt aaaaataacg ttctaaagcg atagcgtttt ctttgaaaat tactataaaa
     9241 acagcagcga acccgtttga gatcctctgc tgttttttat taatttgaag atccagaact
     9301 ttcattgtcg ttggaagttt gcggatgttt gtccttccaa gtcttttgtg cagcttcttt
     9361 cgcatgattt tcagcttctt tcgcatcatg cacatctttt tcgacatctt cataatccaa
     9421 gcgagtaatt tctccgccta attccatcat taccaattga ttcagtgggc gattgtcctc
     9481 ttcatcagca atcaagatca gtcctgtagc tccttcaccg attttatttc ctacaaagtc
     9541 gaacacggtc tgggcatctt ttatttcttt ggcatcttgt gtcgcaccga tcattcctcc
     9601 ggcaaaccat cctaataaaa tccctagcgg accacctaat acaccaacca acattccgat
     9661 taatccaccc gtagaagatt tgttattacc ggtaaagtcg ataaaatctt ctattttgaa
     9721 ttgatgtgtc ccattaggat catggctgac aactgccatc tgttctcctt taatcgaacg
     9781 aacagcatgt aactttttga tttctgaaaa tgcctgatat gcctggcttt ctacttgaaa
     9841 atttaaaatg attactcgtt tgctcattaa aaacgcccct ttcatacctt cttgttattt
     9901 attttacctt taattgaaaa ataagaaaag aaaagcactc tttttttcca ataaaatagg
     9961 gctgtgacaa aagtcgtatc acagctcctt tttccgaata aacggcgttc ttgaagctga
    10021 tcacttctgt cacaacctcc cttaaatctg aatctatatt ttttggtatt tgtttttatt
    10081 tgcttgccta ttcgacaaat tcaaattcaa actcaccgat acgtacaagg tcgccatctt
    10141 tcgcaccgcg tgcccgaaga gcttcatcca cacccatacc acgtaattga cgagcaaaac
    10201 gcatcactgt ttcatcatga tcgaaattcg tcatttggaa taatttttcg atcttctcac
    10261 cagataagat ccacgttgca tctgaatcac gatcgatttg gaactcagga ccttctggat
    10321 tgaatccata gtgtacagtt tcttcttcca gttcttcttc gtacaatggg aattctggcg
    10381 ttacttctaa caaatctgct gtagcattca ataacgcgtc caatccttgt cgagtcactc
    10441 cagaaattgg aaatactggg atgtcatcag caaattcatc ttctttttcc ttgttcagct
    10501 gttctttgaa tttcactaga ttttccggtg catccggcat atccattttg tttgccacga
    10561 tgatttgcgg acgctctagc aagcgtaagt tataagtaga taattctttg ttgatggcaa
    10621 gatagtcttc ataaggatct cgtccttcca tcccactcat atcgatcaca tgtaaaatta
    10681 ctcgtgttct ttcgatatga cgaaggaatt gtgtacccag cccaacacct tgcgaagctc
    10741 cttcaatcaa gcctggcaag tctgctgcgg caaaacttct gccatcactt gtagtaacca
    10801 ttcctagatt aggtacgagc gtcgtgaaat gataagcgcc gatttttgga cgagcagatg
    10861 aaataacgga tagtaaagtc gatttcccga cagatggaaa acctacaagt ccaacgtctg
    10921 ccaatacttt caattctagt tcgatttttc gctcttgtcc tggttcaccg ttttcagcaa
    10981 tttccggtgc aggattccga ggagaggcaa aacggatatt ccctcgtcca ccgcgtccac
    11041 cttttgccac aactagtgtt tgaccatttt caattaaatc tcctaaaaga gcaccagtct
    11101 ccgcatctcg gacagtggtt ccttgtggta ctttaacata ggtatcttct gaaccacgac
    11161 cgtgcatccc tttactcatc ccattttcac ctggctgcgc tttgaaatgg cgattgaacc
    11221 gaaaatccat caatgtacgt aacccttcat cgacgatcaa gatgacatcg ccgccacgac
    11281 caccgtctcc gccagccggt ccgccgtcag gaacgtattt ttcgcgacga aatgcaacca
    11341 ttccgtctcc gcccttgcca gctttcacat cgatcgttac ttgatctaaa aacatggaca
    11401 taattaagtc ctcctattat tcatttattt atttgactgc ctgaatttcc acaataaaca
    11461 aaagtcttac ttattttatc ggtgaaatct gattttgtct atcatttcag caaaaagatt
    11521 gtttaccttc ttcttttttt atcatttggt aaggtttcca accttttttc tgctaccaag
    11581 tataattttt tgacaaaata ttctttgcgt agcagaataa cagtgtatta attgtattaa
    11641 ttgaggatgc cgtttcacag cacccctaat aaaggaggaa cttttgatat gacagtaaaa
    11701 gtaggtatta acggatttgg gcgtattggc cgtctggctt tccgccgcat caaagaagtc
    11761 tcagatgata ttgaagtagt agcaatcaac gacttaacaa gtccaactat gttagcacaa
    11821 ttattgcaat ttgactctac tcatggtact tatcctggca cagttactgc aacagaagac
    11881 tcaatcgtag tggatggcga agcaactcgt gtttatgcag aacccgacgc aagcaaaatc
    11941 ccttgggcaa aagaaaacgg tgtagatatc gtactagaat gtactggttt ttatacatct
    12001 gaagaaaaag caaaagcgca tttagatgct ggtgtcaaac gagtagttat ttctgcccca
    12061 gctggtgcta tgaaaaccat cgtctataac gtcaatgatg atacattaga tgaaaacgac
    12121 aaaatcatct cagcaggttc atgtacaaca aactgtttag cacctatggc ttatttctta
    12181 aataatgaat ttgggatcga agttggtaca atgacaacag ttcatgccta cacttccaca
    12241 caaatgctct tagatggacc tgtaaaaggc ggtaatttgc gtgctgctcg ttcagctgct
    12301 gacaatacta tcccacattc tactggtgct gcgaaagcaa tcggcttagt tatccctgag
    12361 ctacaaggaa aattacaagg acacgcacaa cgtgttccag tcgttgatgg ttcattaact
    12421 gaattggttt cagtattgaa aacaaaagta actgctgatc aagtaaacga agcaatcaaa
    12481 aaacacacag ttgacaatcc ttcattcggg tatgatgacc gtcaaatcgt ttctagcgat
    12541 gtcattggaa caactcaagg ttcgatcttt gacccaacgc aaacagaagt aacaactgct
    12601 ggtgatttcc aacttgttaa aactgttgca tggtatgaca atgagtatgg atttacttgc
    12661 caaatgattc gcttactaga aaaattcgct aacttgtaat ttgatagatc aatcgataaa
    12721 agaagccttt gactggatcc agtcaaaggc ttctttcttt tcatccccta cttttaaact
    12781 cgttcaatga acatattggc ttaaaaattg ttggactact tgctgatatt tttcaggatc
    12841 tgtcttgtat gcttccgcat gctcggctcc ttttacaaca tacttttctt ttggtccctt
    12901 cgttgcacga tagaccttat ctaacatttc aaatggtaca aatgtatctt cgtctccatg
    12961 tataaagagc attgggcgct ggtttttctt caattgttcc actgcatttg cttcaccgaa
    13021 aaagtagcca gcacggactt ttgtcactag actcgttact tgcataagcg gaaatgctgg
    13081 aagactaaac atatcgtcta gctgataagc aagctcatca tgaacggaag aataaccgca
    13141 atcttcaatg attgctttca cattatctgg tagtttttct ccgctagtca tcataactgt
    13201 tgctgctccc atgctgacac cgtatagaac gatttcttgc gattttccat tgttttctag
    13261 cactttgttt atccactgta catagtcttt acgctctggc catccaaatc caatgtaatc
    13321 cccttcgctt tttccatgtc ctcttgcatc aggaacaagg acattgtaac ccatatcatg
    13381 aaacatcttt gcgtaattgg tcatcgtttc agcgtttccc atatagccat gagccacaag
    13441 agctgttttt tcacttttcg tttcagcagg aagataaatc gctgataatt tcagaccgtc
    13501 ttttgaagtg atggtccaat cgcttcggtt ttcttttgaa ctaaaccatt tatgtgcatc
    13561 cgtttctttg gatgtctgtg ttgtaccagg tgtatcttcg tctaaaaagt ctttctcaga
    13621 aggcacaaca gcataattat aaaaataatt ccctgcaaac actaacgcaa caatcagagc
    13681 taaagcgacg cctaaaagga ttttcatcca aagtttcata aatcatccat ctcatttctc
    13741 ttttttcata taaccttgaa ttagtataat caaagaatct ctactatgca atgttcattt
    13801 ttataac
//
LOCUS       DACEUZ010000075        13538 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.075, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000075 DACEUZ010000000
VERSION     DACEUZ010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13538
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.075"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(62..1009)
                     /locus_tag="IXG44_001775"
     CDS             complement(62..1009)
                     /locus_tag="IXG44_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HAQ1658496.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            1199..1468
                     /gene="rpsN"
                     /locus_tag="IXG44_001776"
     CDS             1199..1468
                     /gene="rpsN"
                     /locus_tag="IXG44_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAQ1658497.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(1644..2006)
                     /locus_tag="IXG44_001777"
     CDS             complement(1644..2006)
                     /locus_tag="IXG44_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAQ1658498.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(2017..3138)
                     /locus_tag="IXG44_001778"
     CDS             complement(2017..3138)
                     /locus_tag="IXG44_001778"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HAQ1658499.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYIHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(3154..3504)
                     /locus_tag="IXG44_001779"
     CDS             complement(3154..3504)
                     /locus_tag="IXG44_001779"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HAQ1658500.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGKVWVSIT
                     HTNETAFAQIILED"
     gene            complement(3634..5145)
                     /locus_tag="IXG44_001780"
     CDS             complement(3634..5145)
                     /locus_tag="IXG44_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAQ1658501.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQSETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(5467..6819)
                     /locus_tag="IXG44_001781"
     CDS             complement(5467..6819)
                     /locus_tag="IXG44_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HAQ1658502.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKDFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVPDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAVKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(6972..8048)
                     /locus_tag="IXG44_001782"
     CDS             complement(6972..8048)
                     /locus_tag="IXG44_001782"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HAQ1658503.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            8244..9989
                     /locus_tag="IXG44_001783"
     CDS             8244..9989
                     /locus_tag="IXG44_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HAQ1658504.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDSVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLKKEEQTQASRKTEIVHEKEFGSFAFRASTSTEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(10238..10705)
                     /locus_tag="IXG44_001784"
     CDS             complement(10238..10705)
                     /locus_tag="IXG44_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HAQ1658505.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFCQALKKAIDQGLPSTEDSR"
     gene            complement(10689..12062)
                     /locus_tag="IXG44_001785"
     CDS             complement(10689..12062)
                     /locus_tag="IXG44_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1658506.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGIMYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDAAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(12383..12871)
                     /locus_tag="IXG44_001786"
     CDS             complement(12383..12871)
                     /locus_tag="IXG44_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1658507.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEAFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(12893..13495)
                     /locus_tag="IXG44_001787"
     CDS             complement(12893..13495)
                     /locus_tag="IXG44_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAQ1658508.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         3863 a         2938 c         2458 g         4279 t
ORIGIN      
        1 ttttttgtct ttgcccctac aaaaatgttt cagctttttt cgttttttat tcctgtattt
       61 cttatttttc gaatgacagg atgattttcc cagcaggagg atgtccttca actagctgat
      121 gtgcttggat gacatgctta agggaagcag acagattcat tgaaataaaa acatgaaagg
      181 ctcctttttg gtaggctttg attatgccag caaaggcttc tgcatctaga tattcctttc
      241 ttggaacaaa cgattcataa aagccttcct ttttttgccg caaatctaga tctgggaggt
      301 catttaatgc aacatacctg ccgcctggtt tcatgatctg gatgccggtt tctcctttga
      361 tacttccttt cgtcgcatcg attacaaggt ctgcctgatc agcaaattgc agacctggat
      421 ttgttttatc ataagcagca aatgctgaag ctcctaattt ttttaccttt tcctcatttt
      481 tactggatgc actggttaag attcggattc ccttttcatg gagaagctgg atcagacttg
      541 acccgaccgc acccgatgct cctagtatca ttacagtatc tgtcggctgg atttccgtca
      601 aatggttgat tagattataa gccgtaattc ctggcgtgac cattccggct gcttcttccc
      661 aactcatttt atctggaatt tttgctactt ttgctgatgg caacacgact tcttctccgt
      721 aagtcccgcc gactgcatgg acagctactc gatcacctac atgaacagtt gtgacatctg
      781 aaccaatctc tgtcacaata ccagcgccat cattacctgg tacataaggg aatttcaatg
      841 ttcgtatctc tttcatttct cctaaacgca aggcaacatc ataaggattc acactgaatg
      901 cttttatttc tacacgaaca tgtccagaac cgacttctct gggatgagca tcgatttctt
      961 caaaaacatc ttgtgcttct ccgtaaccgt taattgcaaa tcttcgcatg gtcaccatct
     1021 ccctttttct ttaagtttac gtttatgcct ttattttgac aaaaaaaatg tcttttcgtt
     1081 ttggcgcatg attcaaaaca aaatagttgt cttttttcgc gtaacgtgtt aaattattta
     1141 tctaaaagcg taaagattac gatttacgct aataaaaaat aattggagga atttgttgat
     1201 ggctaaaaaa tcaaagattg ccaaagcaaa aaaacagcaa gaaacgattg aacgctatgc
     1261 tgctttacgc caggaattaa aagcgcaaaa agattacgaa ggcttgcgca aacttccttt
     1321 agatgctcgt ccagaacgat tgaaaaatcg tgatttgatc gatggacgcc ctcgcggata
     1381 catgcgtaaa ttcgggatgt cccgtataaa attccgtgaa ttagcacata aaggtttgat
     1441 tccaggagta aaaaaagcca gctggtaatc caagtctggc tgtaatgtaa aggaggaggg
     1501 cgcatggacc actccttgga tcatcaaaaa agatacgttc gcttcttcta gcagaaatgc
     1561 tggtactaga agcagacgta tcttttttta cgcacagctg gtcctttttt atatgtaaag
     1621 gctgcatggt tacttcgcta tatttattta gtaacaggag tcagtgcttc gatacccaca
     1681 ctaattttca atgctcggtc aacttctgcc ataatgggtc gatccaaatg gcagaccttt
     1741 tctttcaatc ttgatttatc gatcgttctg atctgctcca acaggataac ggagtctctt
     1801 tctattcctg ttccagtagc tttgatccca atatgagtcg gaagcttagg tttagccatt
     1861 tttgccgtta tggccgcaac aatgatcgtc gggctaaaat ggttacctag attattttga
     1921 ataaccaaaa ctgggcgtgt tccgccttgc tccgatccca cgactggaga aagatcagca
     1981 aaataaatat ctcctctttt taccatagga tcctccttat tcttgatatt ctcttggtat
     2041 ccgctgtcct aatccgcaag ccacttcata gtggatcgta cctaattgat ctgctatatc
     2101 ctgcatagtg atttctttgt ctgcattttt gccgataagc gtcacttttg ttccgacagg
     2161 aaattcttga ggcaatcgga tcatcagttg atccatacat acacgcccaa tcgtttcaca
     2221 gtagttccct tctaccaata gagaaaaacc ctgcatcttt cgtggccagc catctgcata
     2281 accgatcggt atcgttccga tccactctgc ttcaggagtg atatatgttt ctccatagcc
     2341 gattccttca cctgctggga gttttttgac ttgaattaat tcagaaacca gctcaagtgc
     2401 tggctgcagt ggataaactt ccggtagtgc gtgcccagag ggattcagtc cgtacattgc
     2461 tactccataa cgaatcatat tgccgattgt ttcatcatgc cataaagccg ttgcactatt
     2521 gctgacatgt atatatcgag gaagttcggg aagttttttt agcacttcta taaagcgttc
     2581 tttttgcaga ttccaatatg tatcatctgc ctgatctgct gtagaaaaat gggtaaataa
     2641 accttcccat tctaatacac gactttcttt gatgaatgca gctgcctctt ttacttcttc
     2701 aggtgtacaa aatccgatac gacccatccc agtgtctgct tttagatgaa tcgaaagagg
     2761 cgtctctagc ttacttttct ctaaaacagc agctgctgcc tgcaaccatt cccgtgtacc
     2821 tgcagttact gataaatcat gagcaacagc tacagtcagt gcgtcgtaag gaaccatatt
     2881 caagataagg atgggctgag taaatccagc ttctctcaac tcgactcctt catctaaatt
     2941 tgaaacacaa aagcccgaag ctccacctgc aactgctgcc tcagctgttt caatagctcc
     3001 atgaccgtat ccgtttgctt ttactactgc aaacaattct tttccttgcg gaagtcttgc
     3061 aacttcattt gctacatttt cagttatagc ttttttatgt atcactgctt tcgttgggcg
     3121 gtgccaagcg acaaccatcg tttttccttc tttctagtct tccaaaataa tttgtgcaaa
     3181 agctgtttca tttgtatgag taatactgac ccatactttg ccttcatgag gtgaacgcgt
     3241 cacaacaggc tgaccgtttt cattttttaa aatctcgaca tcttgaaagg taacttttcc
     3301 gatcccagtt ccccaagctt tagagaaagc ctctttgcaa gcgtaacgcc cgcctaaaaa
     3361 ttctacttgg cgatgaaaag gcaaggactg gaatagtttc atttcatcag aggtcaagat
     3421 tcgtgcaatg aattttggtt tttcttcgat cagccgagtg atcctaggaa gttcgactgc
     3481 atcgatccca attcctttta tcatatgctt ttctcttttc aataaattta gaacactctc
     3541 attctaccaa attataacca ttttgccaca aataagtcca caaataacaa aaatgaaagt
     3601 ttctcttaaa aaagagaaac tttcatttta tgtttaatcg ttatttgaac ggattacaaa
     3661 tccgcgtttt ttatcgttgt gacgtttacc gccatctttt tggctgttat tatttttgct
     3721 ataaccgcct tttgatttgt ttccacgata attaccgccg tcacgacgat tacggttgtt
     3781 tccgccacca cgtttgccat ttttattgta tccttttttc tgcattggca atgggcgttc
     3841 tggtgtgatc ttcactggca ctgcatcagc tggatctttt gctgtcgttt ttagcaaaag
     3901 tgcaactaaa tcttgtgcag aatattcttc taataattta tctgctgttt gcaggtactt
     3961 atctagacca ttttctgcaa gttttgtttc gatttgttca actgcagcac ctaattgacc
     4021 tttgaatgct tctttttcgg ttggtggacg taaagtcgtc atccgttttt tcgtcaagtt
     4081 ttcaatcaca tgcaagtagt ccatttcatt tggtgtaacg aaagtcacag acataccgcc
     4141 ttttccggca cggcctgtac gtccgatacg gtgaacgtag ctttctggat cttgagggat
     4201 atcatagttg tacacatgag tgacacctga aatatctaat ccacgtgcag caacgtctgt
     4261 cgctacaagg atatctaaat gaccgctctt aaatgaacgc aatacgctca ttcgtttttg
     4321 ttgagacaaa tcgccatgga ttccttctgc acggtaaccg cgtgcttcta atccgcgtgc
     4381 caattcgtct acacggcgtt ttgtacgtcc gaagacgatt gttagttcag gtgtttgaac
     4441 atcaaacaag cgagtcatga tatcgaattt ttcgtattct tttgcgcgga catagtattg
     4501 atcgatcaag tcagctgtca tttctttcgc tttgatcttc acatgatgtg gatttttcat
     4561 gaatttaacg ccaatattct tgatagctgg cggcatcgtt gctgagaaaa gtaatgtttg
     4621 tctttgatca ggaacttgtg aaatgatttt ttcgatatct tccaagaagc ccatattcaa
     4681 catttcatct gcttcatcca aaactaatgt ttgaacggtt ccaagtttca atgtgtggcg
     4741 gttgatgtgg tcaagcatac gtcctggtgt acccacaacg atatgtggac gatctttcaa
     4801 gccacggatc tgacggccaa tatctgctcc gccgtaaacg gcttgtacac gtactttttt
     4861 gtcgcgccct aaacgatata attcttcttg tgtttggata gctaactcac gtgttggtgc
     4921 gataactaaa ccttgtaatt cgtgacgatc tggatcgatt ttttctaaca ttggtaatcc
     4981 aaatgcagct gttttccctg tacctgtctg tgcttgtcca ataacgtctt ttccagctaa
     5041 tgctagtgga atcgtttctg attggatagg tgttgcttct tcaaaaccag cgcgttcgac
     5101 tgatttcaac aattctggtg ataattctag ttctttaaat ttcaaatgaa tcctcctata
     5161 tggttgttaa ctgaggatgt agcttttttg ctaccccgtc gatttgttcg gtcaaagtgt
     5221 attgtttctt tttgacagtg gccacgcacg tttttatcca ttcttagccc ctgtggcacc
     5281 accgaaaaat cactttgcga agtttaggca ttagaaaagc caaacgggtc gcgttcagct
     5341 ttatcattat tatgacatct ggcttcaaca agttgtttct atggcaaaac cagtccaatt
     5401 tctcttcatc tttcggcaat aggttattct aacatagttt ttcgttttct gcaagtttac
     5461 agatttttat ttcatttgct gaagctgttc taccacttca aataagccca tgctgttgct
     5521 gccttttagc ataatactat ctgttgcttc aagattttgt tttacagcat caatcaattt
     5581 attcttttct ttttcaaaat agaacacagg tagttggggg tatttttggt tcaattcttc
     5641 ttttagtgca tgcatttgtt ccccataaag gaaaatcatg gcgaatttct caggatcgat
     5701 atgttcactc atttgcgcat gcatatttaa agactctttc cctaattcca gcatatctgc
     5761 taacacagcg attctgcgtc cttttgttgg tagattttta aaagtatcta ataccagtcc
     5821 catggctgtt ggatttgcat tatagacatc gctcaaaata gctgcaccat ttcctgccgt
     5881 tagccattcg gttctgtttt gagtgataga aacatgggcg agcccttggc gtatctcagg
     5941 tatggacagt ccaaagaagc gcccgatcgt atacgcaatc aaagcattcg ttacattata
     6001 acttccaggc agcgggatcg taaatgtttc tccttctact acaaaatcgg tttgtgcttt
     6061 tccttttgct gtcacttccg catgaacatc gccatttcca ataccgaatg ttgtgactgt
     6121 ttgtactaat gttgccgtta aaggtgtcag caacggttca tctgcgggca caacgagtaa
     6181 tccgtctggt acaagtccgt cagtgatttc cattttggct ttagcgattc cttctcgcga
     6241 cccaagattt tctatgtgtg cttcaccgat cattgtgatt gctgcagctt ttggttgagc
     6301 cataagtgaa agttccgtga tttcattggc gtgatccatg cccatttcca agacgatctt
     6361 ttctgtatct tggggcatgt gcaaaatcgt gtaaggaaga ccaatattat tgttgtaatt
     6421 tccttgcgtc ttatatgttt tgtatttttc agacaagaca gacgcggtta gatctttcgt
     6481 ggtggtttta ccattgcttc ctgtaattgc aacgacttct gcacctactt tttccaagta
     6541 ataggttgcc aattgctgca tggctttcaa aggatctgct acaggaataa ctggaaaatc
     6601 ttttggagct tgttcaacag gcaagctcca aaatgaagca gatgctccgt ttgtgatcgc
     6661 ttgctggata aattcatgtc catctcgcgt accagcaaga ggaacgaata aacttccttc
     6721 ggttatcttc cggctgtcaa attcgacatt ggtaatcttt gtcagtgcag agcattcgct
     6781 gcccacggct tgtgcaattt cttgtattgt tagattcatt gagatctctc ctaaaaaagg
     6841 ttgtgaaaaa agcgtttggc ctaaacggcg ggttcgcatg actctcactt ccaagtaaat
     6901 ttctctattc gtatttagaa gctagaatga acgcggttac gcaatcactc cagcttcttc
     6961 tttatttctc tttattcatt tttttcaaaa aaagattgac gctgatggta tcgattcatt
     7021 cctaattgaa tcagttcttc aatcaaatca ccgtatttca agcccatatt ttcccataag
     7081 agtgggtaca tactgaactc cgtaaatcct ggcatagagt ttaattcatt caggaataat
     7141 tcgtttttat ttgtcaaaaa gaaatcgcac cggctcaatc cgcttccacc taacatcgtg
     7201 taagctaact tcgcgtactc ttgcgctttt tgataaactt cttccggcac ttcggctgga
     7261 atctgcattt cgattttatt attgatatat ttggcttcat aatcatagaa tgctacgtct
     7321 tttacgactt cgccaggcaa agtcgtccga acatcttcat ttcctaatac agcaacttcg
     7381 atttcgcgcg cttcaattcc ttgttcaacg attgctcgag aatcatactg ataggctgtt
     7441 gctaaagcat tttgcagctc ttctcggttt tctgcctttg taatgccgac actagaaccc
     7501 atattcgcag gtttgacaaa catcggataa agcaaagaac cttcacattg atcaaatact
     7561 tttttaggat tttctttcca ttgattctta agtactggta cataaggaac ttgcggcaca
     7621 ccagcagctt gtaaaatata cttggtcatg attttatcca tggcacatgc actggtcaat
     7681 acgcctgcgc cgacataagg catattcaat gtctctaaga agccttggat cgttccatct
     7741 tccccgtttg gcccgtgtaa aactggaaaa acgatggctc cttcttcttt gatttctccc
     7801 ggattgatca cttttcctgt aaagccttcc tctgtctgtc cacttgggtc ccatgaaaga
     7861 tgcaagacat ctttgctagc aggtttttct gttagtaatg gacctttgac ccattgtcct
     7921 tcttttgtaa taaatacgag ttgaacttgg taataattat aataaatggc atttaaaact
     7981 gaaaatgcgg aaagaatgga aacttcatgc tctgcgctgc gtccgccata tagtaaagta
     8041 atcttcaaaa gattgtcctc ctgtattcta aatcgttcag tgatttactc tcatatttta
     8101 gcacaaaaca aaaataaacg aataacttat ttataaaaaa acgagttggt ttctaaaaga
     8161 acaaacgtca tttatttttt gtcagctaga ttatttgtcc cgttttttat aaaaagttat
     8221 gataaagaaa aggagggatg atgatggcgc aaacaaatcc acgtagccgc ttggcagaaa
     8281 tcattcgagt gttcatcaat tacaatgtag tgccaaactt tgtacagcaa aaaaatcccg
     8341 aacaggtaaa aaaagccttt gaagaactag gccccacctt cattaagata ggacagatgc
     8401 tttctgttcg agaagatttg ctttcttctg ccttcactca aacattcaaa acattacaag
     8461 atagtgtacc tagcgacaca ttttcaacgg tcaaaaagac gatcgaaaca gaactttctc
     8521 tttcattatc agatatattc gatgattttt ccaaatcgcc ttttgcctct gcttctatgg
     8581 ggcaggctca tcgcgccaag ctgaagaacg gtgactcggt cgtcgttaag atccaacacc
     8641 ctaacatcgc cgaagagatc cgacttgatc ttcaactatt tgaacgagcg atccctttga
     8701 tcaaatacat tcctgaaact agcgtagttg atctaaaagg ggtactccaa gaagtaaaac
     8761 gttccttgat caatgaaatg gacttcttaa aagaaagtca aaatggcgaa caattctatc
     8821 aaaagaacaa tggctggaaa gagatccgca gccccaagat ttatgacgct ttttgttcga
     8881 aaaaagtgat cgtgatggaa gaaatgtctg gtaaaaactt gaaccattta atgaacgccg
     8941 aaaacaaaac tgaaactttt atcactggaa ttcaaaacaa gcagctgaaa caagaagttg
     9001 caaagctttt agttgagaac tttatgaaac aggtatttga tgatggtttt ttccatgctg
     9061 atcctcatcc agggaatctg ctattccatg tactaaaaaa agaagaacag acacaagcta
     9121 gccgaaaaac tgaaatagtc catgaaaaag agttcggttc ctttgctttt cgagcctcca
     9181 cttctacaga agaccctgtt gcgccatata ccatcaacta catcgatttt ggtatgatgg
     9241 ggcatctttc tgcaggactt aggcaaaaac tcacacaagc cgttttagcg ctgtatacga
     9301 aagatgctta tcgaatcgaa aaagcggtcc ttcgactttg tcagcaagaa ggctcgtttg
     9361 atgaaagtcg gttccaccaa gaactgacaa gttttcttga acagtattat gattctccta
     9421 ttgatgaaat caacttgcaa gaagtattca cacacgtcgt gacgatttgt catcaaaata
     9481 atctgcagtt cgatcgtgat atcacgcttt tgctgaaagc atttgggaca ttagaagggg
     9541 tgatccgcgt ccttgaccct gaagtttccc tcatggaagt cgcttcgcct tttgcccaac
     9601 attattttct cactcatctg gatgtagaag atacattgaa acaaagcgga ttagacttat
     9661 tggaagggat gaaggctgcg cctaagatcc ctcaacagct ccaccatcta ttagaaatgt
     9721 ggacttcggg acaaggaaaa gtcaatttgg aattgaaaaa gcaagacaaa ttattgagca
     9781 ggatcgaaag tatgatcaat cggcttgttt tcggtatgat tttagctgca ttgatcgtag
     9841 ggtcctccct gcttgtccaa gcagcacctg tagaaaacgc agaggtcgtt tccttattag
     9901 ggatatttac gtatgctatt gccgcattcg tcattatttt tttggcaatc gatgcattga
     9961 tccaaatgta taagaagagg aaaaagtaga aaaaggttgt gacagaaaga atcagcttca
    10021 agaaataaga agtgattttg cataaattgt ttctcaaatt tttgtgaaaa ttcggcttat
    10081 ttttcgaaga ggctacttct gaagcaccga gaataaaaaa tgtgaaaaag ctgttctgca
    10141 cccagtaata agaacaatca aaaaggaaca tggagcacat ttatcctgtt tccctgttcc
    10201 ctttttgatg tattcgagca taaataaaat accttcgtta cctactatct tctgtacttg
    10261 gtaaaccttg gtcaattgcc ttttttaacg cttgacaaaa agacatatga tctttctccg
    10321 taaagggttt tggacccttt gtccttatgc cagcttttct taactttcca ctatgataac
    10381 gttgttctag aagcgtgccg atattcgctt cgtcaaattc ttttccagag tgatggaaca
    10441 ctcggacgcc cttcggcaat acaagtgaag ctaaaccata atcttgcgtg atcaaaaagt
    10501 cattcttctt aaccataccg acgattttat aatccgcact atcagctccg cggtctacgt
    10561 agacgatttg gacatgatta gcgtattctt tggtcgtata atggtccaca ctggtcacga
    10621 taaccacgtc aagaccatag gattctccta attttatgac atcttctttt actggagaac
    10681 catctccatc aatgaatagc ctcattcttt ttcgccttct tccataaatg ataaacagtt
    10741 actaatccgc tcagtaaaag accaacattg atccattttg ccaagataag cagttgtaca
    10801 ctttctatcg gcactgattc atggttcagc gtgatcaata atagatggat tggctctccc
    10861 atttcttttg ctaaaacaaa caaaagcgtc atcaataaaa actggtggat cacactccct
    10921 ttattttgag tagtatactt gacaacgatc cggcgatcca ttggaatagc tgcatcagtt
    10981 tgataatatt ccttatttag ccagctgcct gttactacgt gccagaaact aagcagaaag
    11041 acacctatcg caaaaccatt tggaatcaag aggaccaata aagaagaact cagtccaagc
    11101 aattgcatag ccaatggaga ccatgttaaa cgtttcaaaa tccatggtcc gactattttt
    11161 gctgcaatca agcctgctgc ataaggcaaa tacataaaaa tcgaaagttt ttctaatccg
    11221 tacattatgc caacataaaa actgctaaat aaaaaaagga aattccctag cattccattt
    11281 agaaaagtaa acagatttag ccagcttggc aacttccatt ccttcttctg gtgtcctaaa
    11341 acaaacacga acaagatgaa cagcagcaag aaaccataaa tagcaagatc aaaaagcctt
    11401 tcatcgaata acaatcgggc atttctcaaa agaaacaata agccaaagaa tatcaaaaat
    11461 aaaacaagct ctcgcgtagc taccagattt cttttgatgt ctttgaaatc cagttcgtaa
    11521 cgcggataat gactaaccgt atggtaggcc atgacgtaaa aaagtgtata aatagtaaag
    11581 cttgctgcaa tctgagtcgg tgcaggcaac tcaatcgctt gatacaggag aaatagccag
    11641 atagctgcaa ataagtactt tttcccatcc atattgataa aaccttgcgt cttttcgtga
    11701 aaattgaccg tggtattagc aggtacaagc catgctgcac tcagccctag caaaacactt
    11761 gaaaaataaa ataaaggaaa ataaaatacg cctaatattc caagcaatga accagcacca
    11821 ccaatcagca gagcgatcat cagcaacgtg taactatcta gaccaaatcg tagactgttg
    11881 actaaaaata gtccagtcat ccgaaaggca taaaacagcg taaatggcaa aatcgtcagc
    11941 caattatgcg agtctgttag tcccaaaaat aaaagataag gaataaaaat aaccgtgtta
    12001 cttaagataa agggcgttgt ttgtgtaaaa ttacgcccaa atcgtttgag tttttcgaac
    12061 atgacaatgg ctccttaaac agtcttataa ctaatctgct cttcgatttt agcattaaag
    12121 gggaaataaa ccaattttac agaatatatt cttgtaaaaa aaatacagaa agaagcataa
    12181 gcttctttct gcatttttac ggtacttgat tactcaagca gacaagccct tctccacaaa
    12241 agtcttgccc gacacccctg catccggcag gtgataaaga tagaaatcaa ttatcctgtg
    12301 cctccaaaat caattaggag ctctgtaaac cagagtcctt ctcgacccgt cgcataaatt
    12361 tactttaaca cagttcctat gtttatgcga gagctttttt cattttataa tcgaccattt
    12421 gaaatccttg accgatatct gaaataagtt cttctgtaat ctcaaatcct tcatgcttat
    12481 aaacctcgat tgcttgatga ttcccctgat tcactcgcaa aaagatctct ctttcctttg
    12541 tttgcgcagc aagttcttca taccagcgaa aaactgaaga agtcaatcct tgaccacgta
    12601 ctttatttag caaatataat ttacttaaat aaatttttcc atttatctct tcataagcag
    12661 tgtaaccgac agtcttttct tcgcttttta ataaaaagta ggacacacct tctgtcagtt
    12721 ctttttgaat ctgttttttt gactgataag ttttcagcat gtaatccact tgtttttgac
    12781 caatgatcgg cacaaaagct tccggccata cttcttgtat gactgccatc agctcatcca
    12841 gttcattttc tgttactttt attttttcca tacacccacg tcctttttta cattataaat
    12901 gaccttgcgc ttgatggaat aaaaagagca tgatcatgct atacacaccc accgaccacg
    12961 gtttcaagaa aatcacaatc tgcataaatt ccctaaaaga taatttcgtc aaaccagcta
    13021 ctagacagat caagtcgtct ggcgcaaagg gaagcagcaa ggtaacaact agtacttttt
    13081 taatatcttt cgtttttctt gtcagcaatt catcaaattt ctgatacttt tttggcgata
    13141 aaataagctg gacaaaggct acgccatagt aacggacaag caaaaatccg attgcttccc
    13201 caatcactag gccaatacaa ctatataaaa gcccaaatcc attgccaaac atcagcatgc
    13261 ccgctactga ggagatccct cccggcaaaa taggaatgat cacttggatc acttgcaaca
    13321 gaacaaatat catcactgca tacttgccaa attgctggat aaacgcttgc aaagacgtgc
    13381 tcgatcgaaa aatcccatga tgataagcat aaagaatcaa cacaaagaaa aaaaacagac
    13441 caataatggg catgatctga agacattttt gtaaaactga tcctttttta gacatctata
    13501 ttccccacat cttttttcat ttattaaaac tgcctcct
//
LOCUS       DACEUZ010000076        13194 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.076, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000076 DACEUZ010000000
VERSION     DACEUZ010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13194
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.076"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(135..1046)
                     /locus_tag="IXG44_001788"
     CDS             complement(135..1046)
                     /locus_tag="IXG44_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ1658509.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(1449..2027)
                     /locus_tag="IXG44_001789"
     CDS             complement(1449..2027)
                     /locus_tag="IXG44_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1658510.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            complement(2047..2715)
                     /locus_tag="IXG44_001790"
     CDS             complement(2047..2715)
                     /locus_tag="IXG44_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1658511.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEAKELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            complement(2730..3800)
                     /locus_tag="IXG44_001791"
     CDS             complement(2730..3800)
                     /locus_tag="IXG44_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1658512.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAVGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            complement(3971..5335)
                     /locus_tag="IXG44_001792"
     CDS             complement(3971..5335)
                     /locus_tag="IXG44_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ1658513.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQAIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEHTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            complement(5332..6891)
                     /locus_tag="IXG44_001793"
     CDS             complement(5332..6891)
                     /locus_tag="IXG44_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1658514.1"
                     /translation="MRRKLLLLKSEMLGGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     ISVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQICYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFTITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            complement(6947..9427)
                     /locus_tag="IXG44_001794"
     CDS             complement(6947..9427)
                     /locus_tag="IXG44_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1658515.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLADIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISTSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSVQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYEAKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            complement(9411..9746)
                     /locus_tag="IXG44_001795"
     CDS             complement(9411..9746)
                     /locus_tag="IXG44_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1658516.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPVPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEEKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            complement(9760..10794)
                     /locus_tag="IXG44_001796"
     CDS             complement(9760..10794)
                     /locus_tag="IXG44_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAQ1658517.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTIPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNEFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            complement(10884..11564)
                     /locus_tag="IXG44_001797"
     CDS             complement(10884..11564)
                     /locus_tag="IXG44_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAQ1658518.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGSLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            complement(11848..12951)
                     /locus_tag="IXG44_001798"
     CDS             complement(11848..12951)
                     /locus_tag="IXG44_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAQ1658519.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHRLIIPHVLRDVELPDTTTHFDEEMLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDNGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
BASE COUNT         3783 a         2719 c         2267 g         4425 t
ORIGIN      
        1 tccttgtgac gcggagttaa acggcaggct cacaacctct tccttaaacg gtgttcaaaa
       61 gcttgcatct gcgataataa gcccggccaa acgaaaaata tgaggagcta tttttgtttg
      121 gctgggctta tttattactg aaaataagtg gtcaatgctt gtgtcaaact tgcagcaatc
      181 tgctgctgat aactgtctgt tactaattcc tttaaatcac tatcattatt catataaccc
      241 atctcgatta aaatagaagg ttgaagattt tctcgaagaa cttgatagtt cccagtggag
      301 attcctcttg aatccagagg cagatcagtg aaggagtcag caattgtctg tgctaatgtt
      361 tcctcttgtc catagtaata atatgtcgtg attccagttg cttcattcgc atattccgta
      421 gagtctgcgt gtaaactgat aaagacatct gccttttttt cttcactgta gtcgcatata
      481 tctccaagct gtactaattc atctgtagta tgggtcaaat aaaccgttgc tccagcatcc
      541 tctagcgcct tttttacttt tatggctgta cttaatgtta tatctttttc ttctgtttgt
      601 tcatcattgc ttaatgcccc agtatcattt ccgccatgcc caggatctaa tacaatcact
      661 gcatcagata gctcttccgg ttttgtttgt atattctttg tgactttggt tccatcttcg
      721 agggctaaat atccagtata gccatcattt gtcttgatct gataagcatt ttttgtttca
      781 gaaagaaact ccgccagctc tccgccgttt atagttgcaa gtggggtagt tagcgagtca
      841 tctgtatata gtgtacggtc attttttccg attttgatta atgtctcttg ttctgtatca
      901 tcaaatgatt cctttgtctt tgtaaccgta ctgttcgtac tgctgaccgt ttgtccaact
      961 atcttttttt gaccaaaaac atctatttga aaacagataa taatgataat gattgcaaat
     1021 acaaaagctc caattatttt tttcattctt gactctcacc tcgtagatcc agactaaggg
     1081 tcaaaactta aaatcagctt aagtacgcaa aaaaggggct gtaaaagata taaccgtaga
     1141 aaataacatt actgctaaaa tgattttttt catttttggc atatcctgaa atctcctttt
     1201 gactattcgt ggtgattcat ctacccaata aatagtttcc ttcattctac aaacaattaa
     1261 tttcttcgtt attaaattaa aacacttcct ggaacaccga cataataaat aaaaatatac
     1321 cctgataatc atgagagaat aacaaatttg tatttatgaa aaactaccta tataaaaaaa
     1381 acaagatata acgatcttcg tcatatcttg cttcttcacc taactatttt tctccctata
     1441 taacagcttt acgcttctcc taatacacca ttcaaatata aatcgatcaa ttgcgccaca
     1501 tttagcggtt cttccacagg tcttttcgac cgagtgatgt aaggtgctaa cactaagaaa
     1561 aaatgccttg ctgcgatttc ttttgaaatc ccatttctta gcacagattg gtttgctgaa
     1621 aagatttctt caaaaggttc acgataattt ttcttccata gaaagaaaag atgatctctt
     1681 gtttcctttg aaagattgct ttcaagatcg tgaagcatca gaaacaaatc gaccagatgt
     1741 gtctcaatca aaaactgcgc catttttgtc aaggttactt gaaatcccgt ttcgcttgtt
     1801 tgaacgattt ctctcaaagc ttttccaaca tcttttaagt ggttttctaa aactgccgta
     1861 tatagttttt ctttatcacc gaaataatga tagagattgg gttgggtgat cccaactttt
     1921 tgtgcgatct ctcttgttga agtgccgaga tatcccttgg acataaacaa gtcagtcgct
     1981 tctttcaaaa ttttttgctg tgtatctttg ttgtatcgat ggaccatttt tccacctgct
     2041 ttttggttat tctttttctt tcaatacacc atcttccatc ataaatattc tatcgcattt
     2101 gtcaatcagc cgtgtatcgt gagtaaccat gatcgttgct ttatttttgt cttttgattc
     2161 ttttgccaag atatcgacta cttctcgtgc cttttctgaa tccaaactag cagttggttc
     2221 atctgctaga ataatggacg gatcattgta caatgcacga gcgattgcga ccctttgccg
     2281 ttctccacca gatagttctt ctggatactt gttgatcaac ttatccacgc caagctgctc
     2341 aaataattct tttgcttctt tttgacgatg ttcatgtttg atcttatcta ctaataataa
     2401 ctgctttttt actgtcagaa aaggaaccag attggaagct tgcaaaataa agccaatttc
     2461 tttaaaacgt agtttaacgc gttcactttc tttttgatta ctgaattctt gatcattgat
     2521 aaatacagag ccactgctag gcgtctgcaa tccgcctgcc aaagtcaaaa aagtactttt
     2581 acctgaacca gaaggaccga tgattgcaac aaattcccct ttatcgatac tcatggttgt
     2641 ttctttcaat gctttaacta cttggtcgcc atcttggaaa cttttttcta cttctttaaa
     2701 ttctattgct ttcatgctct tcctccttct tatccaattg ccttcaacgg atcgattttt
     2761 acgattgtcc gaacagaaaa tagtgcgcct aacacagcta ctaccagcat caacaagcta
     2821 atgacgccaa aaaacagcca attgctttgg aatggaaccg catcaggtag aacaagggat
     2881 gttcccactg ttcctaatag accaagaaga ataccaacaa atgataaaat gaatgtttgt
     2941 gccactacag aacgcgcaat aaatcctcct gtgatccctt gtgctttcat tacaccaaag
     3001 atattaatct tttgcatcgt cagtacataa atgaaaatcc cgatcacaat agccgcaata
     3061 acgatcaaaa atccgatcat aaatccaaaa gtcaatactt gcgcattata tccaggtaat
     3121 tcattgatga atttagaaat tttgatctgt tctaaatctt caggtaaatt actgatttta
     3181 cctctgacaa ctattgcgtt aatccgtgca ttttcacttg tatcttcttt ttcgaagcgg
     3241 atctcttggt aagcattgat ggttgtatag agaactggtg agacattgaa tttggcatga
     3301 tccgtaaagc cagttagttt aaacgtcttg tcactaccag ataacttaac tgtatctcct
     3361 acggctagac catattcttc tttcaaacta atatcgccta ctgcctcatc atcgttatca
     3421 aaagcttttc cttcaacaag attgggcatg atgaattgat ttttatcgat accgaagaag
     3481 ctaacattga tttttccttc ttctgtactg tctttactag tcacgactcc aggtgtctgg
     3541 gcaagatacg ccactttccc accttcgact tcttctgcta tcttcttagt aatcatggac
     3601 atccctagat ttgaatttgc atctttcgaa agcacaatcg aatctgcctc ccacttgtcg
     3661 actgctgttc gattatcttg agctaagcca taagctaacc cagtcaaaaa aaataccagg
     3721 taggcgatca aaaacatcac ccctgcaact aacgcataac gtaactttga atgcataatt
     3781 tcatttaatg ctaaaaacat ttaactcctc ctcctgaaat aatcatttta tacaacaatt
     3841 ttatcacttg ataaattttt ggcgaatatt atgctcaaaa aattcttccg ctatttcatt
     3901 atatgaattt tttattctcg attgattaat atttattata tccccacaca ataaaagacc
     3961 gtgatatttc tcacagtctt ttatctactc tattgctatt tgctctctta gctagtactt
     4021 taaaagctaa ctcattgagg tagtgaaaac gagaggtgtg ttcagacaga aatccaaaat
     4081 cctcaactgc tagctgtgga atcgtagttt tgtgtttcac gatatccgca agcaggttca
     4141 aatctgataa taagaattct tggcttatca gctgtccgcc aattatttct aatgtatccg
     4201 gatggtagat cagctttgca tttactgttt tgttttcttc atcgtattgt ggaaatagat
     4261 caaattctcc tgaacacgaa ccggtatttt gttccaagaa tggcgcttca tcttctgtca
     4321 gccctacagt tcctaaaaag tagtctataa tgactgctgc tgttatcttt tgtctgaggt
     4381 cgtaagcggt tggctgacca ctcaaagtta gcgctactgc tcttgccata tgtacagctc
     4441 gagtaactaa aggtaaaaag agatgccgtt tatttttgtc aaaactgact gggacgatat
     4501 ctccaatcgc atagatgtcc ggatcacttg tctgcagata ttcattgact atcacagtct
     4561 catcctcata caaagccact ttttctttta acaatgtcgt attaggacga gtttgggcag
     4621 aaaaaatgac catatctgcc tttattgctt gagatagaag ttgtacggat actactttct
     4681 cttctgattt tttccaatcg atcaagaatt catttggata aaaattgatt tgcttagttt
     4741 gcagctgatt ttgaaaactg ttgatcatat ccttatcgaa atatcttgat aaaacattcg
     4801 gcatataatc aactagatga ataatgtatc ctttatttct caaagcatct gctaattcca
     4861 atccaatata accagcaccg aagatcacta cttcttttgc ttcttttaaa gcggcaagta
     4921 cttgcttaga ttgcactaga tttttatagg tgtagacatt ttcagcttca atttgtttat
     4981 acaaggtgac aggagaggaa cctgttgcta cgattaattt atcataggtt atggtctcgt
     5041 tttttccttc tttggatgcg aggatgagct gatgtttgtc tggatcaagt tcagttactc
     5101 tccattcggt gatcagtttt gccccacttt cttgaagagt ccgcatgtta tgggcaactt
     5161 cagctaaact acgtatcttt ccttttaaat aaaggttgat tccatttgaa acatagccta
     5221 gtccatcttt atgatcttca tcgatcagta aaacttctgc tttcggttga agttttctta
     5281 gatttagggc agctgtgatg cctgcatggg aagcaccaat gatcacgatt ttcatgtttt
     5341 attcatcctt aaattcaatt tttctaaata acattctttt aaaaattggt tcaaataggg
     5401 gaaatcataa ttatattcgt tgctagtgaa tacaaaaact ttagcggact tctgtgacgg
     5461 gtactctttc aattggctag tcaaaatgat atcatactgc ttttctttct cgtattcgac
     5521 taaatcatat ttgatcgtat tcagcaattg ttcctggatg atttctttca cgatatccgc
     5581 cattagcctt tcataaggaa gatcataagc aatttgcaga cgtaattcta actgcttgat
     5641 cagcgtccgc aaaatagatg catagcgagc aaataattca aagcgggcat tttcctcgtc
     5701 tacttcagta aaatcaacaa tagcaaatgt ttgtctgacc aatctttcac ataaaaccag
     5761 gaattgttta tcttctactt cagttaaccg ctgttttaat ccttcttggc caaaaatatt
     5821 catccatcct ctaaaatagg caaaatgagc gtgattttgc atcactgtca acgcaatctt
     5881 ttgcgaaatc tcttctggaa aaacataaaa agagaaatat tcttctatcg attgtttaaa
     5941 cgttcgattc aattgagcta ctacgtcgtc tttgtatttt tcgcttatca aaaactcctg
     6001 gacatgcgag ttatgtgtat ctaatgtaaa gttactgatc atgaaaagat aaaaaaccaa
     6061 cgtttcattt tgattccaag gaaaggcata ttgaaagaag taacgggtca aaaatgtatg
     6121 gaggtccaat aaaaattgtg tcgtttgaaa caatccaaca ggaagattgc tataatctgg
     6181 tttggatttt ttcgtacagc gcattcgctt cttcataatg gaaagccata tttcaaattt
     6241 caatcgtcct tcttctgtaa tagtaaaact aaactctttt tgcagcagat ctaaaaaagt
     6301 gtttcctgat aaatcactgt attctttttt gttttcaaga taaggacgtc caaaccaaaa
     6361 taattggaaa taaaaataac aaatttgact ttctggaccg gtcagccgcc cgctttttat
     6421 tttcagatga aactcattta aagaatcatt taattccttc actctgcgag acaaagtagc
     6481 cgaactgata ttaagttcca tgacaagttt ctgccgatca aatgagccat tttccaacag
     6541 ataaagaagt atctgatatt ttgtagaata gcgcaagtag caatgataat aaacatcgac
     6601 tgctgctata tttccatgtc tgattaataa aatattccct tcttggataa caatttccaa
     6661 ttgtttgata ttttccaagt tagcttgtaa ataaaacaga tcttctttca aggtagcccg
     6721 atcaactgat aagcgggtaa gtacgttatt aacggaaatc tctccttttt cctctaataa
     6781 aagttggaaa agtttgattt gccgttgttc atttttttca agcaaactga aaagttccaa
     6841 gtccactcct ccaagcattt ctgattttaa cagcagaagt ttccttctca tccttgaaaa
     6901 agaggatctg tgacagttat cacagatcca tacaaattct aatcacttag ctggttggcc
     6961 cagctgtcag ttcccatgtg accatgcctt gatacgttgc gccaggttga gcattcgtac
     7021 tgctatttac ctctaagagt aaaccatcgg tatcgggaaa gtcaacgaga acctctcctt
     7081 tagctgttcc atcactctca taaatcaacg tgttttgacc ttgctgaatc aattgttttg
     7141 atccagaatg attgaagtat aattttgttc cgcttaattc ttgaccttga tcattcttaa
     7201 ataaagaagt caatgtccct gtcagttgcc aaggagacgt gcttggaagc cgagtatccc
     7261 gcacatcaaa agaaaaatcg ccttttgctt cataataact tgtcttcaac ggtttgggga
     7321 gatcgttacc aaatgtaaaa tcatctggta cattaactaa tgttaattct tgtttgggga
     7381 caagaattgt tttagcagat gtttggacaa ctgggttagg aagcttagca ccatctgcta
     7441 atgtaatatt gctggaaagt acagctgcct ttgtcagtat gctgacggat tgtacagaag
     7501 tatctacagt atttaattga tagttgtaag taagatgaat ggtatctcct attttgctca
     7561 atagatctgc tgggacagaa actccttgtt gaatgccggt atctgcgata gttgtaccat
     7621 ttaattttaa ggaaccattg atataagtag tattttcagg aagtgagatc gtgatttttt
     7681 tgtcggttat cgcatcccca gattttgtta acgtgaatgt cacattttct tgaatcgtat
     7741 ctcctgatag agcagacagg ggatctcctg tctcaggttg tctgctaagg ttttttagct
     7801 gactatctgc actcacagat ggaaaattag ccactgttat ttgaggaaga gtggtgattc
     7861 cagcttgttc agactgccat ttgtacgcaa aagacggata catgttagta agtacatcgt
     7921 ccaccaagcg ataatcaaaa taatattgcc ctgaagccaa tgcaggtagt gtagaagtag
     7981 ctgtatttac acccccgtta tttgattgga tcgtttggaa aagttgatct gtccatcctg
     8041 tcgctaattt ttgggttcct ttcgaataaa gcctgtaatg gatcgaaccc ttttttagtt
     8101 cagttgctga ccaattggcc gtgacttggt acgatgcaga agaaaggagc tttccccact
     8161 gactatcggc tgttaagttt gttggtaaaa ccgttccgtc tagcttgtac tgctccaata
     8221 cctttaaagc aacaggagaa agcaatccgc cttgcgcatc tttcccactg tacacgatat
     8281 tggctgtctg attcaatcca cttgtgcttg ctggagtatg agtcagtatt gctccttgtg
     8341 taacgttttt tcctgtaaaa tagttgacta cgcgatcaaa gcgactaaat ggcaactgat
     8401 caaaacgtcc ttcagtcagc cccatcgtca atgtccgact attggtagag cctggaaggt
     8461 tctggttatt tccatccacg tattctgtgg tatcagcaaa tgtattatat tttcctgttg
     8521 aatcacttgt tgagatgtaa ctattccacg tatttatctc tgcacttgca gaaaatgcat
     8581 atgctcctgg attcaagttt ccgccagtta atttaggata aaatctacct gcagctggag
     8641 ctaaaatcga attgaacata cgatcttgga taatattatt cgaatcataa aaatcaacag
     8701 aaaaaacggc ctttttagac atagaaggtt gtaatttacc cttggtcaaa attttgtttg
     8761 gactcgtatc atatttatct ccgttttctg tcatcttcgc gacattagct gtaacatcaa
     8821 attgaagcgg gatatcgtcc acgttgaatt cgcctcgcgt aataatagca ataatatctg
     8881 ccaatagagt atttgtatcc cacgacccca tcccaaaacg caaggtatgg cttcctgctg
     8941 ataataaact accgctagta accttgatgg gggcaccggc aatatacaag taggcgttgt
     9001 tccagtcaat tgcttttaat acatcactgc ttgatacatg ttccggcaaa ttgatgatcg
     9061 ccacaaatgc aaatccttgt gcagtgatat tcaccaaatc aagtacattt gttagtttca
     9121 catggctttc ccaatgaaaa cggaccgtgc cacctttcca aggatcattt gaaccatcgt
     9181 tcaaataagt agcagtcaat ttaggcaatt ggttagcccc agttagttgg acaagcttcc
     9241 cgccattgac tgcactacgt ccaccatttg gaggtacgat attttcatac gtgtatggat
     9301 cataagaaat ggcaggaggc gttgctccaa cagcttctgc cgaaggtgaa aagataaata
     9361 tacttaaaac aaaaagcgaa gtaaaaaaga aggagcgaaa aaatgaaaat ttattttgta
     9421 gcaacatgtt catcctcctt ttcctttctt ctccataaca acaaccacgc aagaagccat
     9481 aattctatac caatgatcaa ataaagaaaa cggatctttt cttcattcgt ctccgggaat
     9541 acgccatgtt gattagattc tttataaatc ggtagctgtg tctgctgttt tgatgcgcca
     9601 ttgactgaac cacttggatg cggaactgga cttagatcat aaaatgtaat cgtacctgaa
     9661 cttttggtat ctgcttggac tggctcttgc cagctgaaga acagcaaact tgcaataatc
     9721 cctcccaata agatcgatgt tttcaacgaa atcacctcat catgttgttt ctcctttttt
     9781 ctttttcttg tggacccaca aaaacagcaa aatcaaccat aacactagaa ctgcagcaac
     9841 caatcctgca taaagccatg ggaaagattg ttcttgtact gcaaaaacgg aattttcatt
     9901 tactttttgt gctttttctt ttttgatcgt aaagttttgt gtaaatcgcc attcattact
     9961 gtcgcctgat tttgctatga tttctgcgcg gtaatcgccg gcttttgctt tatcaccagc
    10021 taaatcaaga ttcaagtgaa aattcgaatt tggtgcaact aaaaattcat tagacgtatt
    10081 ttcaatataa agctgatttt cataaaagat cttgctcgta aatatcaaat tgctgataat
    10141 cctaggctga ggattttgga aacgaagctg aacagttggc tttgcgttga cctcgtccaa
    10201 atctcctgat aaaagtttca gatccggttc tatcgtctct tgactttctt gtaaaacgac
    10261 tgcgagatta taagcaatac gatttttgat ttgtgctcct tctgtagcat tttctgtttc
    10321 agcttcttct ttaggtgaaa catgaatacc cgctaataca cgtcccttaa agctttttgc
    10381 tgggattttc acttctatag gtatagttgt ttctgcatgt gctgatacat tgatttcctt
    10441 tttcgcaatg agcaaaacgt cacggacatc aaacggcaat gtctcatcta acgttgggtt
    10501 tttttgacta taatcgatcg ttccaccatc actcgttacc gccggattga cagagacctc
    10561 aaatgttcgt tcttcatctg aagaattgct gataattact tctagtgtct gattttgttc
    10621 cggtgccact aataaatcaa aataaccgat tgctttatct atttggttct ctggaaatac
    10681 cggtttgatt ccaaagtctc ctgctgaatc ctttgcttgg ctgattgacg gaaaaatgaa
    10741 aaatagactt aaaatagaaa aaaacgaaac aaacagtcgt ttactttgat tcattgtcca
    10801 ccacatcctt tcaacaacaa ctgcggtaaa aaatccttca cctatagtat tttcaccgca
    10861 gtttgttgaa tttatttcat actctatgct actgggccag ccacgaggtt ccaagtcaaa
    10921 tcagccgtga atgttccagc tgctgctttc ggcactttca gtgtgatatt tgctggatca
    10981 taaacagaaa gccatgttcc cagaccttga tcttttgctg ctgtaaatat tggtgtcgct
    11041 gcacttgctt tactcaaccc agttacagtt tgactagttg gtccagtgga ttcgttatct
    11101 gacgtacttt taacagtgga tgttccaaaa tctaattcag cgccttgcaa aacttgtgaa
    11161 ccgtttacaa aatctgaaac cgaaactgtc acgttccacc cttgtgcttg gccatctgca
    11221 cctacacctc gacgatctga tacttggaga taaggtgtat cgttttttga agcgtctact
    11281 tgatacgttt ttgttccgct ttcaatctcg tgtgtaccaa atggcaattc aggaactaca
    11341 tccaatgtta aagaacctgt ctggccagtt gctggatttt ccggatctgc tggctctact
    11401 ggttttgaag gatcagtagg gttaagaggc tcaacggtat ttgttggttc agtaaaggta
    11461 atatctcctt ttgaagtcgc ttgaccctca gcaaaagctg gtgttgccaa gccgcctaga
    11521 agaagtgttg aaagtaataa tgtcccaatc atgttctttt tcataataaa tcactccttt
    11581 ttcttagtga cctaagtata gtagagacag tatgcaaaaa atcctcatga attcatccgc
    11641 ttacttgaaa ttatgaccct ttttgtataa aagagaaaaa aacaataaaa tcgtttacat
    11701 gaatattctt ccttttttat taagtatgaa aaaatatttc aattttattt tttcgtttca
    11761 aattattcta ttagtgacat ccttgaacta taaaaatagg agtacggcat catctgccgc
    11821 actcctattt ttttcgtcat ttatttctta aataaaacga ttctcacgca attttatttt
    11881 tttgatatct tctactgttt gtgttccagc taattgcatg accatttcca attctgtttt
    11941 aaagaaatca aacacttgat ggactcctgt acttcctcct aaagacaatc cataaataac
    12001 gggtctgcca atagcaacaa gatcggcacc tgatgcgatt gctttgaaaa catgttgtcc
    12061 acgtcgtaca ccgctgtcaa acacgattgg tacacgttta tctactgctt ctgccacata
    12121 ttgcaacgaa tcaaacgcag caggcccccc atctaattga cggccaccat ggttagatac
    12181 ccaaattcca ccagcaccag attctaatga tcgatagaca tcttcttctg attgaacacc
    12241 tttcacgtag actggcaaat cagaatgagc agcgataaat tctacatctt ttggactaag
    12301 tttttgtttg gaagatttat atacagcatc catcgtttgg cctactcctg attgataagc
    12361 ctgaacgatt ggcattggca gcgggaaggt aaacccgttt ctgcggtctg tttcacggtt
    12421 tccgccaact gttgcatccg ctgtcaatac gattgctttt gctccattac gcttggccac
    12481 ttcaagaata tccaaattga ttccattatc tttgctcata taaaattgaa accattgcgg
    12541 tgctttctcc ccgcctgctt ctcttatctc ttctaaagta caagaagcgt aagaacttgc
    12601 tgtatagatc gtcccaaaat ctgctacacc ttttgcagaa gctttctccg ccttcacatg
    12661 agctaatcca tgagcagcaa ctggtgccat gatgattggt gcagtcagca tttcttcgtc
    12721 aaaatgtgta gttgtatcgg gtaattctac atctcttaat acatgaggaa taatcaaacg
    12781 atgattgaat gcccgttcat tttcttgata agtaaataag tcgccagcac cactgctgat
    12841 gtaaccataa ccaccagctg gaatcacttg tgaagccgcg atttctaaat cttccatatt
    12901 gataaaatct actattcctt catttgtacc tgcttgatat gttttttcca tctcaatgcc
    12961 ttctctctat aaaatttgaa tgatgaacaa tatgatagcg tttacacttt ttatattact
    13021 cttcgacatt gatacaggca aataatctgt ttacatttca gccattccag atgtcactac
    13081 tctggaagaa atgattgtaa taaaaataga taaaaaaccg atgggacttt gacaaaagta
    13141 gtgtcaaagt cccttttttt gaataaacgg tgttcaaaag ccattttctc ggta
//
LOCUS       DACEUZ010000077        13007 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.077, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000077 DACEUZ010000000
VERSION     DACEUZ010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13007
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.077"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(26..319)
                     /locus_tag="IXG44_001799"
     CDS             complement(26..319)
                     /locus_tag="IXG44_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HAQ1658520.1"
                     /translation="MELTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(347..1012)
                     /locus_tag="IXG44_001800"
     CDS             complement(347..1012)
                     /locus_tag="IXG44_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HAQ1658521.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(1152..1784)
                     /locus_tag="IXG44_001801"
     CDS             complement(1152..1784)
                     /locus_tag="IXG44_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1658522.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     ALRPDVILMDLVMEEMDGIESTKAILKDCPKAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNDPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(1791..2858)
                     /locus_tag="IXG44_001802"
     CDS             complement(1791..2858)
                     /locus_tag="IXG44_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ1658523.1"
                     /translation="MMGRISKAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLMAISLGVGLIVFLLLSLIQKAKYGKIEEKLRALSSGNYESKLLHQP
                     IPSASDDLYIKDIDMEIAKIKEKMIEVSSELQIMTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLTSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(2855..3586)
                     /locus_tag="IXG44_001803"
     CDS             complement(2855..3586)
                     /locus_tag="IXG44_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HAQ1658524.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKYPRTKFQNFQLILGSLVIFFSLVNSPALWMMVVLAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMVQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAIISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKICSS
                     DYDENPRRLKIITNTLVGDVEVIRI"
     gene            complement(3754..4233)
                     /gene="greA"
                     /locus_tag="IXG44_001804"
     CDS             complement(3754..4233)
                     /gene="greA"
                     /locus_tag="IXG44_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HAQ1658525.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGD
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(4369..5544)
                     /gene="mltG"
                     /locus_tag="IXG44_001805"
     CDS             complement(4369..5544)
                     /gene="mltG"
                     /locus_tag="IXG44_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HAQ1658526.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPNGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELYQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEKAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVQKYVNNS"
     gene            5709..6869
                     /locus_tag="IXG44_001806"
     CDS             5709..6869
                     /locus_tag="IXG44_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAQ1658527.1"
                     /translation="MAKGKHQKKQQISLSFLAVLLLAVSFFSTGIITGWQLTKQPQPQ
                     IYKTEPAQLASNLTSNEQMLASKIDQLLQKSDYIGSIYVRKNNRVILEKGYGYANLRI
                     KESNSPSLYYQIGSIQKAMTALLILKQIQSGKITFDTKLSEFYPQIPGSQQISIKNML
                     YMRSGLHRTASPKKPMSDSEIIQFALHHLKFTGYDTYHYEPLNYTLLTGVLIKLMNQP
                     YETLLKKEIIRPLHLEHTDFYENVKNSPQHAVSYQMSATDDYSKALVEPETDIRNELG
                     TGNISMSVYDLNKFFTSVLSGDIIPKELLFSLWQNNGDKHPYSGGVYSGNDYILAQGN
                     INRFHAVAAMKKDTKDAIVMESNIQSDKNIKLPATDLRNQIYELMEGVNLKS"
     gene            complement(6909..7856)
                     /locus_tag="IXG44_001807"
     CDS             complement(6909..7856)
                     /locus_tag="IXG44_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAQ1658528.1"
                     /translation="MDTILITGGAGFIGSTLANYLGKENKIVVVDDLSMGKKENLDME
                     KQVTFIEGDVSDPQLMERIMKQYQFAYIFHLAAVASVADSVERPLETHRVNFDSALLL
                     LELVRKYQSGLKRLVFSSSAAVYGDEPTLPKKEESVIRPLTPYAIDKFASEQYVLDYC
                     HLYDVPTSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQLAGEKTEFTLFGDGSQSR
                     DFVYIEDVIQALLLVAKEEKALGQQFNVGTGKSTTLLELIHSIDQILGTELALKYEAE
                     RSGDIRDSLADISKIRSLGYQPKFDILNGMERYLKTEID"
     gene            complement(7989..9380)
                     /locus_tag="IXG44_001808"
     CDS             complement(7989..9380)
                     /locus_tag="IXG44_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="HAQ1658529.1"
                     /translation="MLNEKFLKYVKGWYFIYFGFILMLRAIGPYLLLPTRYDTLLFWP
                     AGIAGVVLIIIDAIIAFREKNLKKYDWLLIAFIGAMLVSSLMNLKYGYSENIKLIMWQ
                     LIFFFMIYQFGKDYSSNFFFKVFTWILSLVWFLANLVSLYWFVIQYGIKIPIKYKYYP
                     VRLGWLGNRLFGVYTDPNFGAVISLIVIIIAIYYLVTYKHLNLLFKIFFAINIFIQFS
                     FIALSGSRTTLVCLLIATFVFSFFTSYSSRFLKTNTLKRWLTALVVGLLSIIAMYYVT
                     DITAQAWAHVPHITEIKKDISDSSGTTSSNKVDLKRPDTDGGDVSNLRFGLWKSAIEI
                     FKSKPISGGSPGYYIEYAHDKLPYTLMGKDTLTAHNFIFLVMAATGGLGLLTLLTFLF
                     IQCFKTLRYCFTQPNLVKDKHFFAILMILTIAVSALFITELILVSTIGSFIFWGYLGS
                     IQKTISKEEGVTN"
     gene            9506..9916
                     /gene="tagD"
                     /locus_tag="IXG44_001809"
     CDS             9506..9916
                     /gene="tagD"
                     /locus_tag="IXG44_001809"
                     /EC_number="2.7.7.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate cytidylyltransferase"
                     /protein_id="HAQ1658530.1"
                     /translation="MKRVITYGTFDLLHYGHINLLKRAKQYGDYLIVALSTDEFNWNA
                     KQKKCYFSYEKRKQLLEAIRYVDLVIPEENWEQKVKDIKEYHADYFVMGDDWAGQFDE
                     LEQLTDAQVIYLPRTPEISTTQIKNELNSLNSDK"
     gene            10096..11385
                     /locus_tag="IXG44_001810"
                     /pseudo
     CDS             10096..11385
                     /locus_tag="IXG44_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(11452..12879)
                     /locus_tag="IXG44_001811"
     CDS             complement(11452..12879)
                     /locus_tag="IXG44_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HAQ1658531.1"
                     /translation="MKKTVKDIFYNAIYQVFLIVLPLLTIPILSRRIGSTGLGIYGYV
                     FSISQFLMTVIAVGMNPFRIRNIAKSRKDKKALSLQFWNIYFIQFLIGLSVSFLYIAY
                     IYFFDVKYKNLYLIQLIFIVGLTLDISWFFQGIEEFSKVVIRNTVIKLSSVLLIIIFV
                     STKDDLWIYVFITSITNFLGSLVFWLSIHGKVGKPIFNSNVFKQLWKPAFIILTPQLF
                     MQVYTTLDKTIVGYFVDPTELSYYDQSQKIARIILAVLTSITIVMLPKISKAQAEGKS
                     ENILVYTKKSFDYTIIIALILFSVVFINTKEFVPWFFGENFKPMTINMLISCLIIVVS
                     PIGGVFANQFAIAIEKDKEYAYPMIIGAIVSIIGNVAFVPTYGAVAATIVLVVVELLV
                     FFFRVFFVRKEIKISYFFGKNVILFFIVNLTLTTWAYYFLPQLFSSSFLDMAIKSIMI
                     FIIYLPFVYFLFPDIRNEIRKITRK"
BASE COUNT         4245 a         2528 c         1948 g         4286 t
ORIGIN      
        1 aaaacttgtg tcaccctctc tttttttatt gttttttaaa gtgataggtt ggctcctcca
       61 ggttgggatc ataatcaaca agtaaatcat atcctttaaa gaaccattca tctgcttcct
      121 cgataaaaaa aagtgtccca tccatttcca ctttctttac aggatgttct ggctgttcaa
      181 ctgacattcc aatcgaaaaa ccatcatgaa cctctgtttt gccgtagact tttccaaaaa
      241 aacgtattcc gtaagtttct ggcagatcaa tttctgagtc aaaccacttt tgtgcttttg
      301 gcgttaccgt taattccatc ccaacaacct actttctctt ttctttctat tgattcatct
      361 tatcgtctag aatatccaca tcgcctgtct ctccaatcac cagtagatta tcattttcgc
      421 gaacatattc atctgctgca ggagaagcga taaccgtttg atctactctt cgtatggcga
      481 ctactgttaa gtcaaaacgt tgcctgaaat ttaattcagc taatgttttg ttgaaaaatt
      541 taggattgga gactcggatt tctgctaaag aatatttatc tgacagctct aagtagtcta
      601 gaatattctt tgatactaag ttatgtgcaa tccgtatccc catgtctcgc tcagggtgta
      661 cgacacgatc tgctccgatc ttctctagta cacgggcatg gtactcattt tgggcttttg
      721 ctaaaatatt cggtacgccc atttccttta ccatcagagt aactaagata ctggcttgga
      781 tatcttctcc aatagcgaca attacatgat cgaaatttct tatacctaat gaacgaagtg
      841 tcatctcatc ctgtgcattt gccacaacag catgagtggc tatattcata tattcattga
      901 ctcgatcttc attactgtca atagccagta cttcttgtcc agcttcgatc agtgtacggc
      961 agatgcttcc gccaaatcgt ccaaggccta caattgcaaa attttgtttc accattttcc
     1021 tcctttagta tcttcttttc tcagtataac aaagcctttc caaaaacaaa agcaagcatc
     1081 attattgtaa aaaagccaag aaaaataata gttattttct cagcttttga ttatcctctt
     1141 tatcatcgtt tttatttcac caaaccatgt ttaaacgcat agatagctgc ttgagttcgg
     1201 tcttctacct ctaacttagc taatatattc gacacatgcg ttttaactgt tttcagtgtg
     1261 ataaacaatt catccgcgat ttcctgatta cttttccctt cagaaatcag catcaaaatt
     1321 tcattttctc gattcgttaa ttcttcatgc aatactggat catttcgccg actcattttt
     1381 tccatcattt ttgttgtcac ttcaggttcc aatactcgct cgccgcgttg cgtagccctt
     1441 atagcatcag ctatttcatg tgcggtagac gtcttcaaca aatatcctgc tgctcctgct
     1501 tcaattgcag gataaacttt ttcatcatca ataaaacttg tgacgataat gattttggct
     1561 tttggacaat cttttaagat tgcttttgtt gattcgatgc catccatttc ttccatgact
     1621 agatccatta aaatgacatc tggtcgaagt gccatcgcct tttcatagcc ttgacgtcca
     1681 ttttcagctt ctccaatcac ttcaatgtct tcttggatag ataaataaga agagacgcct
     1741 aagcgtacca tttcatggtc atctactaat aaaactttta tcatctttat ctatgcctcc
     1801 ttcatcaaag ggaccttgat ctccacgctc gtaccttgcc ctttaaaact aatgattttt
     1861 actgttccac caataccagc tactcgttct ttgatattgt tcaatccata acttcccgtt
     1921 ttggtttcat tcatattaaa tcctgtcccg tcgtcaatga tacgtaataa aagattgtta
     1981 tctattttat gcaaatagac ttctaattca ttagccttag catgtcttaa tgtgtttgat
     2041 aacaattctt gaacgattcg gaacaagtga tcctcaatgg aactagttaa ttttacatct
     2101 tctacatccc attttagcga aatctggatt ttattctgca attccttcaa taactgctca
     2161 atgccttgtt ttaaactttt ttcttctaaa ttgactggac gcagatgaag aagcagcgca
     2221 cgcatctcag actgggaagc gttgatgatc tctgccacca ttttcaactg cttacggaac
     2281 atttctggtg tctcactttt ttctgcttgc tccgtcaatg ccgacatcat catcatcgct
     2341 gcaaataatt gctgtgaaac tgaatcatga agctcacgag ctaatcgatg cctttctaat
     2401 tctaaaatct cttctttggt ttgtccatct acatattgag gacggctagt cataatctgt
     2461 aattcactgg atacttcgat cattttttct tttattttag caatttccat atcaatatct
     2521 ttgatgtaca gatcatccga cgcgcttggg atcggctgat gcaaaagttt ggattcataa
     2581 ttgccagaag aaagtgcacg aagcttttct tcgattttcc cgtattttgc cttttgaata
     2641 agtgataaca atagaaatac gatcaatcct acacctaatg aaattgccat caaatggaag
     2701 ataagcggaa cgtataataa acgcgcacgc agcaattctc cccaccaatg actttggtta
     2761 ctggcataaa aataagtgaa aagtgaaaat agaataataa ggaaagccgc aatccccgaa
     2821 taaacagcta gcattgcttt ggatattctt cccatcatat tcgtatcacc tcgacatctc
     2881 caaccaaggt attggtaatg atcttcaatc tgcgaggatt ttcatcataa tcactgctgc
     2941 agattttgat ctgttcgttt tttaaggaaa actgctcttc ttcaaacaag acatttccta
     3001 ctaatgtcgc atgctctaaa cgaatagcca ctcccagggg tactaagatc cgtgttctgc
     3061 ctatcccttt tcttacaatc acaatattat catcttttgg caaaagtgta ttacccaaat
     3121 cgatgatagt atcaccagaa atgatggcaa tgttgatatc atcccattca tagacgtcgt
     3181 ttccaatacg ttggttacca aataattgct gtcttttacg ctcattatta tgtgttttta
     3241 attgctctgt ttggaccatc atgatctgtt tttttcgcca aaaagcattt tttgtcaaat
     3301 ctattcctga aatctctaca cctttcagcc cgataaataa aacggccaat acaaccatca
     3361 tccataaagc aggactgttg actaagctaa aaaaaatgac caagctccct aagatcaatt
     3421 gaaaattttg aaatttagta cgaggatatt tgcgcatagc tagatatata ttgaaaatac
     3481 cgaatattac taaaagcaaa agtccagtat tgtttacgat ttgccaaaca gcaaataaca
     3541 gcagcagtgc ttcgactacc acaaaaaaac gccaagaact attcatcgct ttttctccct
     3601 cctgacacat tctcatttca tcatacctat ttttttgact gttggctata ggactttaga
     3661 tgtaagatcg ttcagtgaaa cctgcaatac tcaaagagaa aagatctaga gtgcgtcatc
     3721 agtaagatga actctctaga tctttcagtc agactacgct tgttctactt tgacgatttt
     3781 aactaacata tcgccacctg gtgtagcaat ggttacttca tcatttttac gtttgccaat
     3841 caatgcttgc gcgataggag aatcattgga aatctttcca gaaaacgggt ctgcttcagc
     3901 acttccgaca atcgtgtact cttcttcatc tccatcaggc aactcgatga acgtaactgt
     3961 tttaccgatc gatacctcat ctgcatctac gccgtcatta tcgatgattt gtgcaaaacg
     4021 gatcatattt tcaattgttg tgattcttcc ttcaacaaat gcttgttcat ctttagctga
     4081 ttcgtattcg gagttttcgg aaagatctcc gaaaccgcga gcgattttga ttcgttcaat
     4141 gatttctccg cgtttaactg tttttagttc ttctaattct ttttctaatt tttctttacc
     4201 ttctagagtc attggataaa ctttctctac catgtgtaac gtctcctttt ttcgataaaa
     4261 taccgcaaaa ggtaatcttt tgaggaatat agtccaaaaa agattaccac gcttttctta
     4321 aaatgtaaat gtttttctaa aaatgatcaa tgactagccc ctgtttttct aagaattatt
     4381 tacatatttt tgcactagtt ccatatgttc ttcatacgtt ttagcgaaat aaacatttcc
     4441 agtgctcgta tctgctacga aataataata gtcattttct gctggttcta atactgcctt
     4501 gattgccttt tcgcttggat tgtcaaaagg accaggccca tagccggtat tggtatacaa
     4561 attgtatggt gaatctacag ctgtatcttc atacgtcacc aattctttat gttctcctaa
     4621 tgcatataaa acagagatat ctgactgaag tggcatattt gccttgattc ggttaaagaa
     4681 gacttgtgca atatttttac gatcattttc ttttacgcct tctttttcaa ctagagaagc
     4741 caaagttaaa acttcttgta cagtcaaatt tttctgtttg atagctgaat aatagttttg
     4801 catgaccgta tttgtcttgt tcaccatctc aatcacaaga tccttaagcg aagtactcgt
     4861 gtagtaatca taagtagctg ggaataagta gccttccaaa cggtattttg tgttctcagc
     4921 tttagaggca ctttccaaca attctgggaa tttttgatac aactcgttaa agaaagtctc
     4981 atcatttact aaatctaaaa attctttctt gtctttacct gttaccttcg ctacccgctc
     5041 agcaatctga tcgatgtcgt agccttctgg aatggcaatt ttcccatctg caacttttgt
     5101 tggctcagat gtgccgcctt cttgcaactg cttgccaatc tcatcaagag tcatattagg
     5161 cgctaactga taataaccag cttgaaagcc agttaagttt ttaaattttg tataataatt
     5221 gaaaacaatc ccacttttaa taatattgtc tttctccaaa atttcgccaa tctgcttatt
     5281 agaagagccg tttgggattt ccacttggac taattggtcg tccgatttat ctaaaggctt
     5341 caacccgcta tctacataac gatatacggt aaacccgaga aatccgccaa taatcaataa
     5401 tgtaagagcg atcactaaaa tgatcttcct cacgatctta tcttcttttt tacgcagatt
     5461 tctccgcttg tcgattatat ctgctgtagc tgccttactg gcttcttttt tgtctttgct
     5521 tgggtgttgt ttgtttcctg acaaaacaac tcctccttac tatcatcttc gtaactctgt
     5581 ttattataca tgaaatttct actgccgtga attattttac caaatattat gtcaaaattg
     5641 taagtggcaa atgctctttt tgaggattac aatgaaaaat agttaaaaat ggatagaaag
     5701 gatttaatat ggcaaaagga aaacatcaaa aaaaacagca gatctctctt tcttttctcg
     5761 cagttttgtt gctggctgtc agcttctttt caacaggcat cattacagga tggcaattaa
     5821 caaaacaacc tcaaccacaa atttataaaa cggaacccgc acaacttgct tccaatttaa
     5881 catctaatga gcagatgctt gccagcaaaa ttgatcaatt actgcaaaaa tcagattata
     5941 tcggttcgat ttatgtacgc aaaaataatc gagtgatatt agaaaaagga tatgggtacg
     6001 caaatctacg cataaaagaa tcgaattctc cttctcttta ctatcaaatc ggttcgatcc
     6061 aaaaagcgat gactgcttta cttatcctaa aacaaattca atctggcaag ataacttttg
     6121 acacaaagct atctgaattt tatcctcaga ttcctggaag tcagcaaatc tcaatcaaaa
     6181 atatgcttta catgcgctct ggtcttcatc gaaccgcttc tccaaaaaaa cctatgtcag
     6241 atagtgagat cattcagttt gcccttcatc atttgaaatt tactggttat gatacgtatc
     6301 attatgaacc attaaattat acattgctta caggggtatt gatcaaacta atgaatcagc
     6361 cttatgaaac tcttttaaaa aaagaaatca ttcggccact ccatctagaa catacagatt
     6421 tttatgaaaa tgtaaaaaat tcccctcagc atgctgtatc ttaccaaatg tctgcaactg
     6481 acgattattc aaaagcattg gtcgagcctg aaacagacat ccgaaatgaa ttaggcacag
     6541 gaaatataag tatgtctgtt tatgacttga acaaattctt taccagtgtt ctgtctggcg
     6601 atattatacc aaaagaacta cttttctcat tgtggcaaaa taatggtgat aaacatcctt
     6661 acagcggcgg tgtttacagt ggaaatgatt atatccttgc tcagggaaat atcaatcgat
     6721 tccatgcagt agcggcgatg aaaaaagaca caaaagatgc aatcgtcatg gaaagtaata
     6781 tccaatctga taaaaacatc aaattgccgg ccactgattt aagaaatcag atttacgaac
     6841 tgatggaagg tgttaattta aaaagctaag tcgagacaaa aaaaccagca ggatgctggt
     6901 tttttgtttc aatcgatttc tgtctttaaa taacgttcca ttccgttcaa tatatcaaat
     6961 ttgggttgat aacctaaaga acggatcttt gaaatatccg ctaaagaatc acgaatatcc
     7021 cctgaacgct ccgcttcata tttaagcgct agctctgttc ctaagatttg atcaattgaa
     7081 tgaatcaatt caagtagcgt agtgcttttt cctgtaccga cattgaattg ctgtcctaaa
     7141 gctttttctt ccttagcaac aagtaaaagt gcttgtataa catcttctat ataaacgaag
     7201 tctctagatt ggctaccatc accaaataaa gtaaattctg ttttttctcc tgccaattgt
     7261 tttttgtatc ggtccactag aatcgaaata actccgctat atggagagtt aggattttga
     7321 tttggaccat aaacgttaaa aaatcggaca gcactagtcg gaacatcata taaatgacag
     7381 taatccaaaa catattgttc tgatgcaaac ttatcgatgg catatggtgt taaaggacgg
     7441 atgaccgatt cttctttttt tggcaacgtt ggttcatcgc cataaactgc agctgatgaa
     7501 ctaaatacca atcgttttaa accagattga tattttcgta ccaattcaag caataaaagc
     7561 gcactgtcaa aatttacacg atgagtttct agaggacgtt ctacagaatc tgccacacta
     7621 gctacagcag ctaaatgaaa aatataggca aactgatact gtttcatgat tctttccatc
     7681 aattgaggat ctgatacatc cccctcaatg aaagtcactt gtttctccat atccaagttt
     7741 tctttttttc ccattgataa atcgtcgact actacgattt tgttttcttt tcccaaataa
     7801 ttcgctagcg ttgaaccgat aaaaccagct ccaccagtaa tcaatatagt atccatcaaa
     7861 ttcactccaa ttatgcattc tcatacttaa taaagttagt ttaccatttt tatattgtaa
     7921 aaactataaa atttgttgaa gttgggataa ctagttttct tttctacttt atatacctaa
     7981 aaatagtttc aattagttac tccttcttct ttagatatag ttttttggat agaacccaga
     8041 tatccccaaa aaataaatga acctatcgta ctaaccaaaa tgagttctgt gataaataat
     8101 gccgaaactg ctattgtaag aatcattaaa atagcaaaaa agtgcttatc ttttactaga
     8161 tttggttgtg tgaaacaata acgcaaagtt ttaaagcatt ggataaataa gaaggttaac
     8221 aatgtcaata gacctaatcc tcctgtagca gccataacta agaaaataaa attatgagca
     8281 gttaaagtat cttttcccat taatgtatat ggtagtttat catgtgcgta ttcaatatag
     8341 tatcctggag aaccgccgga tatcggctta ctcttgaata tttcaattgc acttttccaa
     8401 agtccaaacc ttaaattaga aacatccccg ccatcagtgt cagggcgttt taaatcaact
     8461 ttattgctgc tagttgttcc tgatgaatca gatatatctt tttttatttc tgtaatatgc
     8521 ggaacatgtg cccatgcttg agcagttata tctgttacat aatacatcgc aattatactt
     8581 agcaatccta ctactaatgc agttagccat ctttttaaag tattagtttt aagaaaacga
     8641 ctactataac ttgtaaagaa actaaaaaca aaagttgcta ttaataaaca aactaatgtg
     8701 gtgcgtgagc ccgataaagc aataaacgag aattgaataa aaatattaat tgcaaaaaat
     8761 attttaaata gaagatttaa atgcttgtaa gtgaccaaat aatatatagc aataatgatg
     8821 acaattaatg atataaccgc accaaaattt ggatctgtat atacaccaaa taaacggttg
     8881 cctaaccacc ctaaacgaac tgggtaatac ttatatttta ttggaatttt tatgccatat
     8941 tgaatgacaa accagtataa tgatactaaa ttagctaaaa accataccaa tgaaagtatc
     9001 caagtaaata ctttgaaaaa aaagttacta ctgtagtctt tgccaaactg ataaatcata
     9061 aagaaaaaga ttaattgcca catgattaac ttgatatttt cgctatatcc atattttaaa
     9121 ttcattaaac tgcttactag catcgcgcca ataaaagcaa ttaatagcca atcatatttt
     9181 ttaagatttt tttctctaaa tgcaataatt gcatctataa tgatcaagac aactcccgct
     9241 attccagcag gccaaaaaag taaagtatca tatctagtag gtaatagtaa atacgggcct
     9301 attgctcgta acattagaat aaaaccaaaa taaataaaat accaaccttt cacatatttt
     9361 aaaaattttt cattcaacat ttttcctcca tccacctatt tctcataaac atttcttatc
     9421 ctttttagga taaactggtt ctttcaattc taacaaaaaa atgctaaact aacaatgtat
     9481 attaatgaat ttcgagaggt gaaagatgaa aagagtaatt acttacggta cctttgattt
     9541 gcttcactac gggcatatca atttactgaa acgagcaaaa caatatggag attatcttat
     9601 tgttgccctt tctacagatg aattcaattg gaatgcgaaa caaaaaaaat gttattttag
     9661 ttatgaaaaa agaaaacagt tacttgaagc tattcgctac gttgatttag ttattcctga
     9721 agaaaattgg gaacaaaaag taaaagatat aaaagaatac catgcagatt atttcgtgat
     9781 gggcgatgat tgggctggac aatttgatga acttgaacaa ctaacagatg cacaagtaat
     9841 ttatttacca agaacacctg agatctctac aactcaaata aagaacgaac ttaattcttt
     9901 aaatagtgat aaataatatt ttttaaacaa tgaaagaaca atatatctgg ataagacgta
     9961 aaaaaatggc tctataattt ctaggactga tagaattttc tgtcgatcct agattttctt
    10021 gtaaaaaaaa cagacataca aaaatttcga taaaatagag tcgtctaaaa catcaatctg
    10081 aaaggaagat tttgtatatc catttctcgt tctatcaaag agcacttcac tattaaagat
    10141 aaaaatattg aaattaattc ttttcaaaaa actttttata aggacaagaa agccatcgta
    10201 atcgaaggta cattagttcg cacttttaga cattgtctat tttgtaaaga aactgcgaag
    10261 cagctcgtga aaaacggcaa gaagatttca atgattatct taaatcgcag tgggaataaa
    10321 taaacctatt tgcgtcttag aaaacaacat taccactacc aatcatgcgg aaaatatttt
    10381 actgctcata cgtatcttgt cgttccttca tgttttattt cgaaacaagt tcattacacg
    10441 atattagaag agttgaccga gcgtcaagcg ttaaagacac ttacgaaacg ttgtgatctt
    10501 tcagtaacga catttcaacg gacattatct gctctatcgg caatgctgcg acagatgcta
    10561 gactatttac ctcattgttt actattcgac gagtttcgct ctctttcaat ttcccatgga
    10621 aagttctcct tttcttgtat gaatagagag acagggaagc tctttgacat tttgtccagc
    10681 cggagaaaaa aagatttgat tgcttatttc atgcgtttta caagaaaagc tcatatgggg
    10741 gttcgataca ttgtcacaaa tatgaatgcc ccttattttt cattagtcaa agagtccttt
    10801 ccaaacgccc aaatcatcat tgattgcttt cacattggac aacacttgaa caagtttttc
    10861 gatcgtgttc gaaaacgtgt gatgaagcag ttgaatcaaa aagacacttc acaagctaag
    10921 tactatcgac aattgaaacc tttgtataaa ttacttctaa aatcagatga cgagttggat
    10981 tacacaacat tcaagaaatg gcagaatttt caatggcgct atttaacaga aagtgaagta
    11041 gttgatcgtc taccctcaat ctcttatgaa cttaaaattg ctaagcagtt tatcaaaagc
    11101 tgcttagcaa ttatcacaag aaacagctag ctacttcgag aaacgaccaa agaagttccc
    11161 tcgaaacttc actatctcaa aaagcctttt tcatatctga aaaaggcttt taactagcat
    11221 tttcgaaaaa gtattctaat aacaaaatta gagaatcttc gcacacatat caaaacattg
    11281 aaacgatttg cttatgggtt tcgcagtttt accaatatgc gcttgtggat ttttctaatc
    11341 aacaggttaa tcagctatgc attaaaagct agtaactctt actaactttg ttgttcataa
    11401 atgaccttat cagcccgatt tgacaaagaa ctactttttt acaacattat tttattttcg
    11461 agtaatcttt cttatttcat ttcgtatatc tggaaaaaga aaataaacaa acggaagata
    11521 aataatgaaa atcattattg atttaattgc catatctaaa aatgaagaag agaataattg
    11581 tggaagaaag taataggccc aagtagtcaa agtaagattg actataaaaa ataagataac
    11641 gttcttccca aagaaatagg atatttttat ttcttttcgt acaaaaaata ctctaaagaa
    11701 aaagactaat aattctacta ccacgagtac gatcgtagcc gctacagcgc cataagtagg
    11761 cacaaaagct acatttccaa taatacttac aatcgcacca atgatcattg gataagcata
    11821 ctccttatct ttttctatag caattgcaaa ttggttagca aaaactccac caattggact
    11881 aacaactata attagacaag aaataagcat gtttattgtc atcggtttga aattctcccc
    11941 aaaaaaccat ggaacaaatt cttttgtatt gataaacacc acactaaaaa gaattaatgc
    12001 aatgataatc gtgtaatcaa aagatttttt tgtgtaaact aaaatatttt ctgatttccc
    12061 ctctgcctgt gctttagata ttttaggcaa cataacaata gtaattgatg ttaagactgc
    12121 caatataata cgagcaattt tttgagactg atcataataa gataattccg ttggatccac
    12181 aaaataccca actatcgttt tgtctaaagt tgtgtacact tgcataaaca actgaggagt
    12241 caaaataata aaagctggtt tccacaactg tttaaaaaca ttagagttaa agattggttt
    12301 acctactttt ccgtggatac ttaaccagaa aactaaactt cctaaaaagt tagtaattga
    12361 agtaatgaac acgtaaatcc acaaatcatc tttagttgaa acaaaaatga ttattaacaa
    12421 taccgatgat aatttgataa ctgtatttct aataaccact ttcgaaaatt cttcaattcc
    12481 ttgaaaaaac caagaaatat ctaatgttag ccctacaata aatatcaatt gaattaagta
    12541 taaattttta tactttacat caaaaaaata aatataggct atatagagaa aggaaacact
    12601 caagcctatt aaaaattgaa taaagtaaat attccagaat tgtaaagata gtgctttttt
    12661 atctttcctt gatttagcga tatttcgaat acgaaaagga ttcattccca cagcaatgac
    12721 agtcattaaa aactgtgata ttgagaaaac atacccataa atccctaaac cagttgaacc
    12781 aattctacga ctaagtatag gtatagtaag taagggtaaa acaatcaaaa agacttgata
    12841 tatcgcatta taaaatatat cttttactgt ttttttcact ttatatccct ctctatcaac
    12901 tcaagaacct tttctgtact tgctcctgtt tcataatttc caatataatc aagtaactct
    12961 tgagaacttt tcaaaagctt ttcttgttgg tttttaaaaa catcaac
//
LOCUS       DACEUZ010000078        12947 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.078, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000078 DACEUZ010000000
VERSION     DACEUZ010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12947
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.078"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(78..150)
                     /locus_tag="IXG44_001812"
     tRNA            complement(78..150)
                     /locus_tag="IXG44_001812"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(115..117),aa:Lys,seq:ctt)
     gene            531..1100
                     /locus_tag="IXG44_001813"
     CDS             531..1100
                     /locus_tag="IXG44_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HAQ1658532.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            1087..1947
                     /locus_tag="IXG44_001814"
     CDS             1087..1947
                     /locus_tag="IXG44_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ1658533.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            2137..2841
                     /locus_tag="IXG44_001815"
     CDS             2137..2841
                     /locus_tag="IXG44_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1658534.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            2846..4681
                     /gene="walK"
                     /locus_tag="IXG44_001816"
     CDS             2846..4681
                     /gene="walK"
                     /locus_tag="IXG44_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HAQ1658535.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            4678..5994
                     /locus_tag="IXG44_001817"
     CDS             4678..5994
                     /locus_tag="IXG44_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1658536.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            5995..6864
                     /locus_tag="IXG44_001818"
     CDS             5995..6864
                     /locus_tag="IXG44_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1658537.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            6919..7728
                     /locus_tag="IXG44_001819"
     CDS             6919..7728
                     /locus_tag="IXG44_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAQ1658538.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(7831..8460)
                     /locus_tag="IXG44_001820"
     CDS             complement(7831..8460)
                     /locus_tag="IXG44_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HAQ1658539.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            8630..9922
                     /locus_tag="IXG44_001821"
     CDS             8630..9922
                     /locus_tag="IXG44_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1658540.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(9998..10660)
                     /locus_tag="IXG44_001822"
     CDS             complement(9998..10660)
                     /locus_tag="IXG44_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAQ1658541.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            10885..11169
                     /locus_tag="IXG44_001823"
     CDS             10885..11169
                     /locus_tag="IXG44_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HAQ1658542.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            11220..12845
                     /gene="groL"
                     /locus_tag="IXG44_001824"
     CDS             11220..12845
                     /gene="groL"
                     /locus_tag="IXG44_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HAQ1658543.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         4315 a         2238 c         2802 g         3592 t
ORIGIN      
        1 ataaaaaaag aacagcgtaa atgttttcga aatgctttcg caccaacatt tacctgttcc
       61 gtgggtttgg cacccaatgg gccgtgaggg gctcgaaccc gcgacccgct gattaagagt
      121 cagctgctct accaactgag ctaacggccc gtacaaaaag tacttaataa tagtagcact
      181 aagaatttta aaatgcaaga ctacatttaa agaaaatcag aaaaatcttt acttccttat
      241 aggaaatcat tcatagatta ttcacatttg ctcgctatta tataagcata ccaacaaaca
      301 aagttcttgc aagagaacaa accctaacac ttttttcatt ttttactcct ttcccgccgt
      361 ctccccggcg ggtctcctgt tcttaacgat aaaagacaag aaggcttagc cttcttgttt
      421 tttgttttca tggaatcccg ctaatcaaat cacctagtta aagcaactag attgaatgtt
      481 tatcataagt ctgatatcat gtttatgaaa tgaaaaaagg agcggttatg atggaaaaaa
      541 tgcgtcagca attaatggag tgggggaagg aactagaaac attccgtctg cctcactggg
      601 aagaactccc tgacttagaa ctttatatgg accaagtcag aacgttagtt gatcgttatc
      661 tttcacctgt gatacaagga gaaaagcatc cattgcttac ctcttctatg gtaaacaatt
      721 acgttaaact aggtttgata ccagcaccag ttaaaaaaag atataacaaa gaacacgtag
      781 catttttact tgcaatcact acgttgaaac aagtattgac tattcctgaa atcaaagaag
      841 ggatattgtt ccaaggaaaa acagtaggaa ttcgtgaagc ctataatcta ttttgcgatg
      901 aacaagaagc agctgtgtgg atggtcagcc aattagcaca aggtaaatct catccgcaaa
      961 aattcgacca ggcaacccct gtagaatata ttgctgtaag agcggcaact ttatcgtttg
     1021 caatgaaact tcttgcggaa aaaacgattg ttctagaaac cgaatattta aaagaggaga
     1081 aaacaaatga aaaacaataa aatcgcaatt ttagtagatt caggtacgga tgtgccagaa
     1141 gaattgataa aaaaatacga gatgtatatg atcccattga aaattattta taaagatcag
     1201 gtatatacag ataaaattga cattacctct gaagagatct atcaacgttt gccagaggaa
     1261 atccctggta catctttgcc ggatggtgag tcgatcacag aaatctttga gcaaatcaaa
     1321 aaagacggtt atgaaaaggt acttgcggta acaatttcca gtggattgag cgggacatat
     1381 aatgtggttc gtttaattgc acaacagcaa gaagaattgg aagcatatgt actagatacg
     1441 aaaaatattg gtattggtgc aggtttttct gcgatccaag cagcaaaatg gctggaagaa
     1501 ggagtagaat gggatcaact gatcagtaat ctgaatgagt tagtcgaaag aacaaaagtt
     1561 ttctttaatg ttgcgacatt ggaatatctt caaaaaggtg gacggatcgg tttagttgct
     1621 tctattgtag gaaccgcttt aaagcttaac ccaatcatct catgtaatga agaagggatt
     1681 tattacacag taggaaaagc taggggacgt aaaaaaagtt tggataaaac ggttgcttac
     1741 gtcaaagaaa aaatcggtaa tgcaaaggtc ttcaacttag ctgttgccca tggggatgcg
     1801 aaagaagaag caattgaaat ggaagcgaga cttaaaagag agttcccaca agcaaaccaa
     1861 atctactttg gccagatttc tccggcatta gtagtccaca ctggtccggg attattgggt
     1921 gtcggtgtac aagttttaga aggataaatc aaaaaaagga aacactgagg tgaaaatcag
     1981 tgttttcctt ttttattgaa gttagaacaa aaaataataa agtattttgc actttcattt
     2041 taattttctc ttttgtttta taggcaaaga tgaaacaaaa gcagaaatac ggtataatga
     2101 gaaggtatga caataatata taagaggtgg ctctttatga aaaaagtttt agttgtcgat
     2161 gatgagaagc cgatttcgga tatcgtcaaa ttcaatttag ccaaagaagg ctatgatgtt
     2221 tatactgcat atgatgggga agaagccttg gaaaaagttg ctgaggtaga acctgatttg
     2281 attttacttg atttgatgct acctaaaatg gacggcttag aagttgctcg tgaggtacgc
     2341 aaaacttatg atatgccgat cattatggta acagccaaag attctgaaat tgataaagtc
     2401 ctaggcttag agttaggcgc cgatgattac gtgaccaagc cattttccaa ccgagaatta
     2461 gttgcacgtg tcaaagcaaa tcttcgtcgc ggtgccacgg ctgcgaagga accagaagag
     2521 gcagcacctg cggaattgac gattggtgat ttgacgatcc atccagaagc ttacatggtg
     2581 acgaaacgag gagaaacaat cgagttaaca catcgtgaat tcgaactact cttttacttg
     2641 gcgaagcacc ttggacaagt gatgacaaga gaacatttat tacaaacggt ttggggttat
     2701 gattattttg gagatgtacg aacagtggat gtaactgtac gtcgtttgcg tgaaaagatc
     2761 gaagataatc caagccatcc aaattattta gtgacacgtc gtggtgtcgg ttactatttg
     2821 cgtaacccag aacaggagta attggatgaa aagaaaagtc cgcttttttc gatcagtcaa
     2881 ttttaagatc gctattacct ttattctgat tttattgatc tcgatcgaaa ttattggtgc
     2941 ctactttata cgtggtttag aacgttccac gatcaataca tttattaaag atatgaatca
     3001 aacggtggaa tcattagcaa caacaatcag cccggaattg aatcgaaaag ataatgcaga
     3061 tgatgaagag gtaaatgcga atatcaaaag atttattgaa aacagtgcga ctagcgatat
     3121 catcgaaata cgggtcgttg acgaaaaagg gatcatccga ggaacaaccg atgtcaatga
     3181 acaaagcgct gtgggaaaga aaaatgatta tgttgatatc aatgatttca cgactaaacg
     3241 ttatgttgct ttagataacg acgataaacg ggtgaatatc aatgttcaac cgatcctgtc
     3301 cccgaccggc gatgccgtta tcggggctct ttacgtcaaa agtaatatcg aacaaaaata
     3361 ttcggaaatc aataacacag cagtcatttt ctttactgcc tcactaattg cggcatttat
     3421 ttcaatgatc gtatctgttt tagttgctcg ttcgattaca cagccgatcg gtgaaatgag
     3481 agaacaagcc attcgaatcg ctagaggaga ctacagtcgg aaagtcaaag tgcatggaca
     3541 agatgaactt ggacagctgg ctgatacctt caatcagtta gctgaacgta ttgaagaagc
     3601 tcaagataca atggaagctg aacgcaatcg tttggatagc gtgctatccc atatgacaga
     3661 cggcgtcatt gcgacagatc gcaggggtaa agtcattacg atcaatgata tggctgtttc
     3721 cttgctagat atcaaaaacg aggaagcaat cggacaatcg attttgacac ttttagatat
     3781 cgaagaagaa tatacgttgc ggaaattatt agaagatcca aacgaaatgg tacttgaccg
     3841 ttcaaccagt acattggaaa gtgatcagat gatcttgcga gtagactttt ctatgatccg
     3901 tcgcgaatca ggttttatca gcggattagt agctgtcttg catgatgtga cagaacaaga
     3961 aaaaacagaa cgggaacgtc gtgaattcgt ctctaatgtt tcccatgaac ttcgtacacc
     4021 gttgacaagt atgcgtagtt atatcgaagc attgagcgaa ggtgcgtgga aagacgagga
     4081 agtagcacca aattttttga aggtaacgct agatgaaaca gaccgaatga ttcgaatgat
     4141 caatgacctc ttaaatctat ctcgcatgga tacaggtaat acccagcttc aattagaata
     4201 tgtcaatttc aacgagttag tgaattttgt tcttgaccgg tttgatatga tggtgggaaa
     4261 tcaggaaaaa aattataaga tccgtcgtga atttacgcaa agagatcttt gggtcgaagt
     4321 agatacagat aaaatcatcc aagtagtcga taatatcatg aataacgcga tcaagtattc
     4381 accagatggg ggaacgatta cttgtcgctt attagaaacg cataataacg tggttttaag
     4441 tatcaccgat caaggattgg ggattcctaa aaaagatctg aaccgagtgt tcgagcggtt
     4501 ctatcgagta gataaagcac gtgcaagagc acaaggtggg acaggacttg gattagctat
     4561 ttctagagaa gtgattaagg cgcatcgagg cgctatttgg gcagagagca aggaaggaaa
     4621 agggtcgact ttttatatct cgctgccata tgaaccttat gaggaggaat ggtgggaatg
     4681 agaatctctg aaaaagtcgt tcgtatagga ctcattttga tggttgcgtt aagcatatac
     4741 ttctcttatg caatctggct gagcccagca ggaaaaagtt caatcaacgt agatgagaca
     4801 aacactcaaa tgatcgagtc tcaaagcaat cgaaaaactt ccgaagtctt tcttccgctt
     4861 catgttacat ggcaccattc gggaatgatc caagaaacca acagtgaaaa tctcgtgtac
     4921 cgattgcaat tgatcatcga taaagcaaga tttggaaagc tttctgaagt agtttcagga
     4981 gacaaatcac agtttgaaca ggtgaaaaat ctgaactctg gttttgagtt ggcctataat
     5041 gcgccttttt tgctaagaga atacaaggaa gcatttgatt tagatttaga tgtgtcgaac
     5101 ttggcaaaag aaagcgaaaa tatgtatttt actagaatac aattcgatca aggaaataaa
     5161 aaaatccgat ttttgaatta ttcgaaccat ctcgtatatg aagcaaatat ttcgttgaat
     5221 tggacagata tcaataaaga attaacagca aaagacacaa aatggacaga aatgacaaca
     5281 gaaccatcga cgatcgacac gcaatatgct acgaaacatt cgatcaaact gaaaaagtat
     5341 agttatatcc tgtcacagca accctataca ctttttagaa atgctttttt ccagaaacca
     5401 gatagcgtga aaaataatga tgaaagcaat gaattgattt tttacggtgg gaatgaaaca
     5461 atgtctatcc attcggaaac gcaagtagtt gattttcgtg gagagctgcc agagaaaaaa
     5521 gaaaatgcta gtgtattttc tgaaagtttc ccctatatta gcaaattagg cagttcttta
     5581 gggaacctca gatattttga ccgcagtgac aatacgatcg attatcggat attcgtggaa
     5641 ggcttcccgg tttttgggac agacagcaga ggacagattg gtgtggaagt tggcggtgaa
     5701 acgaatggtt ctgtgcaagt gaatatccaa accagtttga atacgattca agtgccgata
     5761 ccttcagacg aagaagtaga acttgcaagt acgaatgaaa tcatccagca gttaaaagct
     5821 aagggagcag atagcaacaa gattggttca gtgatcgttg gctatacatg gcaaaatgtc
     5881 aaagaaacga atcgtgtcgt tgatctctta cctgaatggt atgtgaaata taaagataac
     5941 tggtattcag ctaacgacct tctggaaaaa ttacctggat tggaggcgaa ttaattggat
     6001 ttcaaaaaaa tcgaatggat atttttcgtc gcttttttag ggttgaatgt gtttttattc
     6061 agcagttatc atgacgcggt ataccaagaa aacaatgtca ttcgctctaa tcagactgaa
     6121 tcaatccaac aacgtctggc tagcgacgat atcacttaca cggggaagat ttcgtctgag
     6181 aacaagcaag gctattatct tagcgcggag cagaccaatc ttagcacttc attagcgaat
     6241 tatcgcaagg ctggtaaaag cggcttgctg gcaagtggtg tgtttttaga cggtgatgtt
     6301 ttgacgaaat ctttatccag ttcagatgct gaaaagaacg tgatcgatcc agataaaatt
     6361 agtagttcct tggaagattt tttatctaac aaagacaata ttttattcgg taatgattat
     6421 gtgtacacga aaaacttttc tcatttagaa gaagaaccac tagaaatcca agcaagccaa
     6481 gagtatgaag gcatcccatt caatgatgat acagcaaaaa tcgttctttc tttagaaaaa
     6541 aacgataatg attatagcat cacgaaatac atgcagacgc atttatctga tatcgagcag
     6601 ctgcgcgaaa agacggaact gtatacggaa gaggaagcaa tcaatacatt gtacatcaat
     6661 aacaaaatac caagaggatc aaaaatttta tggcgtcagc tagcttattc ctgtattctg
     6721 aaagtccggg aaaaaaatgt gtatgttcct gtttggcatg tagcgatcga aacaagcgat
     6781 aaagtggtcc aaattgagag tgtcaacgct ttcagcaata cgatcgtgac aaacaatact
     6841 gtaccaaaag tggaagatca ataaaaaagc gagtataatg aaaaaagttt acaaagtaag
     6901 aagggaagta ggagtcaaat ggctgaagaa aaaccagctt ttaaaatcag tatcctagca
     6961 agtggaagtt cgggtaattc actttatatc gaaagtgaaa aaaaacggct tttggtcgat
     7021 gcaggactta gcggtaaaaa aatcacttcc ttgatggctc aaattgatcg gacaccggac
     7081 caattagatg gaatacttgt gacgcatgaa catcgagatc atatacatgg cgtgggtgtc
     7141 ttggctcgta agtatcattt ggatgttttt gccaatcagc agacttggga agcgatggaa
     7201 ccactgattg gaaatgtacc agtggaacaa aaacatctat ttgagatggg gaaagtcaga
     7261 acattcggcg atatcgatat tgagagtttc ggcgtctccc atgacgcagc ggctccgcaa
     7321 ttttatcgtt tccataaaga aaatcggtca ttcgtcatgt tgacggacac cggatattgc
     7381 agtgattata tgcgaggtgt cattgaaaat gccgatggtt atttgatgga aagtaatcat
     7441 gatcttgaga tgttacgcat gggaccttat ccgtggaatt taaaacagcg tattttagga
     7501 gatagaggac atctttccaa cgaagacggt gcacttgtca tgactgatgt gatcggtaat
     7561 cggaccaaac gaatctatct tggccatctt agcaaggaaa acaatatgaa agaacttgct
     7621 catttaacaa tggaaaatgt tttgaaagaa aaagattttg gcgttggaca tgattttgaa
     7681 atctatgata cagatccaga tgaagcaact gagctatttt cgatttaaac agttcaatca
     7741 ataaaaatat aaaaagcaga agataaaatc aatcagggtt ggttttatct tctattttta
     7801 tttttttagc atattaaaaa aataaccgta ttatcgactg actacactga tgtgctgctt
     7861 ttcatgtttc tcatccagta tctcatctac taatgcaatc gcgtaatctg ccatgctgat
     7921 cgtactgtcg ccagcagcgt ttctttgtaa ataatcatca cttaacaaat aagtacctgt
     7981 acgtttgcca tgtggatcaa ataaagaagc tggactgata taagtccaat gtagattttt
     8041 agattgtttc aattttaaca atccttcgta catattgaaa gctgttggat agaagggtgc
     8101 atttttaggt acttggtcaa tcatacgagt cgtcttttct gtatctaaga ataacgaaga
     8161 ggcaccccca acgacaatta agcgtgtatc ggttccttca agcatctcaa tcaaatggga
     8221 caaagaagtt tgatgcattt cttcttttcc tttgggagcg ttgaaagcat caacgacaac
     8281 atcaaaagat gcaagatcag tagttgtcaa ctcaaataaa tccttttgta taaacggaac
     8341 atctgatggc aatttttctg gatgacgcac gattgccgtc acgtctaaat ctctggacaa
     8401 tgcttcgtct aaaataaatg aacctgcatg gccagtagct ccaatgagtg caattttcat
     8461 aaaaaacctc cttttttatt cctgtccatt gtaagcagtt gctgaatatt cgtcaaagaa
     8521 tccgattttt gaaaagctca gtgcgcattc ctttcatggt tcgtgatcat tgggtatagt
     8581 taaattaaga aaactgattg ataaaaatag aagttgaagg agataagaaa tgaaagagtg
     8641 gttagatcgt gcctttttac caatgataac atctgttgtg aatcttagac ccttaactgc
     8701 tttgcgtaat ggaatgactt acacgatccc aataattatt gttggctcgc tgtttttctt
     8761 atttgctcat tttcctgtgg attacatcgc tcagtggata gacaaaacag gtttttctat
     8821 gtattttaca caagtatacc aggctacatt tgatgtgata gccttttgga tcgtttttgc
     8881 cattgcatac gcttatgcaa aagaagaaga catcgatcgc ttaccagtcg gcataacagc
     8941 cttgtctgct tatctactgc ttattcagcc actaaatact cagatagata ctgtctggac
     9001 aggtgctgaa gggatactag gagcagttac aactgggctc atcatcggat ggggctatgc
     9061 acgtttagta agacttgcga aagggtggaa gcaaagagag aagatacctg aaaatgtttg
     9121 gcagtctttt acttcattga ttccaatcat tgttatagtc acttttacgc ttatcggttc
     9181 agcggttttt caacagttgt tcagaatgac tgttgttcaa ggagtatgga catatgtcca
     9241 gcttcccatt caatcgttca tagacacgtt gtttggtgtg cttttattag gtttttttgt
     9301 tccgttttta tggttatttg gtcttcatgg atctgcgatg gtcaatgggt tggttagtcc
     9361 cgtcttgcaa gcgaattcat tagcgaatgc agaaatctta gcctcaggac aagagttgac
     9421 ggtggcaaat ggcggacata tcgttactca gcaatttctc gatcagttca tgacggttac
     9481 gggtgctgga ttgacgttag gggctgtatt ttttatgacg gtttttgcga aatcgaagaa
     9541 atacagagaa ttaggaaatc ttgctctttt accagccttt ttcaacatca atgaatcaat
     9601 tattttttcc acaccgatcg tgatgaaccc gatgatggct gttcctttta ttttcgcacc
     9661 ggtcgtttca ggattgatta cctattccgc tttatatttt ggattcgttc ctttatttac
     9721 agcagtccaa gtcccttgga caacaccgcc gatcctctcg ggtttcttag taggcggagt
     9781 gcctgcagcg attttacaag gtatcgtgtt gtcaattagt tttttcattt atttcccatt
     9841 tttgaaaaaa atagattctg caaactgcaa aaaggaaaga cagacaaaaa atgatggagc
     9901 agcagaaaag atgaacgact aaaaaacaaa aatactcctg tcgctttaga ttgggaaaat
     9961 caccaatcta ggatgacagg agtatttata gtcatgttta tactagccct ttttggacag
    10021 ctaattgaac aagaacaact aatgtgttca ttcctacgtg tgtgatcatc gatgtccaga
    10081 tccgaccagt tgctttgtac tctaggctga agaagaaacc taggaaaaag tagattagca
    10141 gatgaccatc attatgagca aacgcaaaca gcaaagagct gatcactgct ccgacccaaa
    10201 aatttgaata tcgactgatg atccccagta tactgcggcg gaatacgaac tcttccatga
    10261 tgggtccagc aacagttgtc gccaaaataa aagcgggtgc ttccatgatc atctggatga
    10321 tattttgtgt attctcggaa ggggttgctt tgccaaacaa tacagattct aacatgacag
    10381 cgatgctttg cagaatcaat gcgacaaaga ttccaaccaa tccataaata acaatctttg
    10441 gagcagaagc tttttttacc gctgattcga tcgctaaggg ttcacgttgc ttaaaataaa
    10501 aaatagctag gacaacggcg cctagtatat acatgactgc agttgctgtg atcaattcag
    10561 acgtagattt tacaagtcct atcgaagcta ggatcaaggg tgagataaaa attagaccat
    10621 agcaaaaaat gctgaggaag ctatattttt ttaaagacat gtaattcctc cttttaaggg
    10681 ataacaagca tgcaaggcaa gcttatatcg attgatttca attatatcat aatttcaata
    10741 agcagctgtc agaataaaat ttcttttctt tgatttaaaa gcttgcaaaa aagagggaga
    10801 atgagtatta ttaaaaatgt aggttagcac tcgaaggtta agagtgctaa cagattaaaa
    10861 aaagagttat cggagggatt tatcgtgtta aaaccattag gtgatcgcgt cattatcgaa
    10921 gtcgcaaaag aagaagaaaa aactgtcgga ggaatcgttt tagcttctgc tgctaaagag
    10981 aaaccccaaa ctgggacagt tgttgcagtc ggtgaaggcc gtttgttaga aaacggtgaa
    11041 aaagttcctg ccgctgtaaa agcaggcgac caagttatgt tcgaaaaata cgccggtaca
    11101 gaagtaaaat acgaaggaaa agaatatctg atcgtcgcag gaaaagatat catggcgatt
    11161 gttgagtaaa tttggaagat aaaaaaataa tcaaaaataa tcaatgaggt gaatcaatca
    11221 tggcaaaaga attgaaattt gcagaagacg cacgcgcagc tatgttacgc ggggtagata
    11281 aattagcaga tacagtgaaa gtaacattag gacctaaagg ccgaaatgtc gtactagaaa
    11341 aatcttacgg ctctccattg atcacaaatg atggtgtgac gatcgcaaaa gaaatcgaat
    11401 tagaagatca ttttgaaaac atgggtgcaa aacttgtatc agaagtagca tcaaaaacaa
    11461 atgatattgc aggagacggt acaacgacag caactgttct gacacaagca atcgttcgcg
    11521 aaggtttaaa aaacgtaact gcaggtgcta atcctctagg cattcgtcgc gggatcgaat
    11581 tggcaacaaa agcagcagtc gaagaattgc ataatatctc aactgttgtt gattcaaaag
    11641 aagccattgc acaagtagca gctgtttctt cgggttctga taaagttgga catttgattg
    11701 ctgatgcaat ggaaaaagtt ggaaacgacg gtgtcatcac aatcgaagaa tcaaaaggga
    11761 tcgaaacaga actagatgtt gtagaaggta tgcagtttga tcgtggttac ttatcacaat
    11821 atatggtaac agataacgac aaaatggaag ctgttctaga aaacccatat atcttgatca
    11881 cagacaaaaa aatctctaac atccaagata ttctgccatt gttagaacaa atcttgcaac
    11941 aatcacgtcc attgttgatt attgctgacg atgtagatgg tgaagcattg ccaactcttg
    12001 tattgaacaa aatccgtgga acattcaacg tagtagctgt aaaagctcct ggtttcggcg
    12061 atcgtcgtaa agcaatgctt gaagatatcg ctattttgac aggtggtaca gtaattactg
    12121 acgatctagg tttagaattg aaagatgtaa caatcgaaaa cttaggaaat gcttctaaag
    12181 ttgttgtaga taaagacaat acaacgatcg tagaaggttc tggagaaaaa gaagcaatcg
    12241 aagctcgtgt gcaattgatc aaaaaccaaa ttgctgaaac aacttctgat tttgaccgcg
    12301 aaaaattaca agaacgctta gctaaattag caggtggtgt agctgttgtt aaagttggtg
    12361 cagctactga aacagaattg aaagaattaa aattacgcat tgaagatgca ttgaatgcta
    12421 cacgtgccgc agtagaagaa ggaatggtct caggtggtgg tacagctcta gtgaacgtta
    12481 tcagcaaagt ttctgctgta gaagcagaag gagacgtggc aacaggtatc aagatcgttg
    12541 ttcgtgcttt agaagaacca attcgtcaaa tcgctgaaaa cgctggttat gaaggatcag
    12601 tgattgttga taaattgaag aacgtggaac taggcactgg tttcaatgct gctactggag
    12661 aatgggtaaa catggttgaa gcaggtatcg tagacccaac taaagtaaca cgttctgcct
    12721 tgcaaaatgc cgcttccgtt tctgccttgt tattaacgac agaagcagtc gttgcagata
    12781 aaccagaacc agcagcgcca gcagctcctg caatggatcc atcaatgatg ggcggtatga
    12841 tgtaagaaaa agcaacttgc ttatctacaa ctgatcatta gtgaaagaca gataaaaaga
    12901 ggctggaaca gaagcgttta actccaagaa ataagaagaa attcacg
//
LOCUS       DACEUZ010000079        12904 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.079, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000079 DACEUZ010000000
VERSION     DACEUZ010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12904
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.079"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(260..979)
                     /locus_tag="IXG44_001825"
     CDS             complement(260..979)
                     /locus_tag="IXG44_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAQ1658544.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            complement(1028..2674)
                     /locus_tag="IXG44_001826"
     CDS             complement(1028..2674)
                     /locus_tag="IXG44_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ1658545.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            2853..3443
                     /locus_tag="IXG44_001827"
     CDS             2853..3443
                     /locus_tag="IXG44_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ1658546.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            3535..5040
                     /locus_tag="IXG44_001828"
     CDS             3535..5040
                     /locus_tag="IXG44_001828"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HAQ1658547.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(5124..6476)
                     /gene="celB"
                     /locus_tag="IXG44_001829"
     CDS             complement(5124..6476)
                     /gene="celB"
                     /locus_tag="IXG44_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAQ1658548.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(6476..6994)
                     /locus_tag="IXG44_001830"
     CDS             complement(6476..6994)
                     /locus_tag="IXG44_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1658549.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(7010..7336)
                     /locus_tag="IXG44_001831"
     CDS             complement(7010..7336)
                     /locus_tag="IXG44_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HAQ1658550.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(7349..9328)
                     /locus_tag="IXG44_001832"
     CDS             complement(7349..9328)
                     /locus_tag="IXG44_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ1658551.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(9349..9663)
                     /locus_tag="IXG44_001833"
     CDS             complement(9349..9663)
                     /locus_tag="IXG44_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HAQ1658552.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            9831..10124
                     /locus_tag="IXG44_001834"
     CDS             9831..10124
                     /locus_tag="IXG44_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HAQ1658553.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(10202..11314)
                     /locus_tag="IXG44_001835"
     CDS             complement(10202..11314)
                     /locus_tag="IXG44_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ1658554.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            11701..11880
                     /locus_tag="IXG44_001836"
     CDS             11701..11880
                     /locus_tag="IXG44_001836"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1658555.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            12074..12244
                     /locus_tag="IXG44_001837"
     CDS             12074..12244
                     /locus_tag="IXG44_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ1658556.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(12668..>12904)
                     /locus_tag="IXG44_001838"
     CDS             complement(12668..>12904)
                     /locus_tag="IXG44_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ1658557.1"
                     /translation="DATPVTFKIEKEQEVAIFLTKENRKVADSSDIRGNNPTSSGNKH
                     LPKTGETIISQFILSILGVLLVFISIKFRKKRNI"
BASE COUNT         4076 a         2590 c         2191 g         4047 t
ORIGIN      
        1 aggttgtgaa ttcagcgttt tggcctgagt attagggaga tcgtgtcctt actatctaac
       61 tctgagtcac aaggaacaat ggggactgta acaaaagtct tgtcacagtc ccttattccg
      121 aacaaacggt gttccagaag tagcttctac ggaaataagc cgaaatttca caaaaaagcc
      181 atccgaaagt cagacggaca agtctgcttt tcagatggcg ctacaactac tctgcttatt
      241 cttttagcta tccattcaat tatttttctc tcacttgttc tagctcttct ttggtcatgg
      301 gtcgatattg ccctttattc aaatctttag gcagccaaaa ttttcccata cggattcttt
      361 ttaaatataa gacttctttt cctactgctt tgaccattcg cttaacttgg tgaaactttc
      421 cttcatgcaa aatcagacgg ataattgaag tttctgtctc ttgatctact tgatcaattt
      481 ggagttttgc cggcaatgtc tgctcatcgc catcaataat cagtccttct gcaaataaac
      541 ggatatcttc ttctgtcatt ataccgctaa cctcagctag atattctttc tccacatgtt
      601 ttttaggtga tagtaactga tgggcaaaca ccccgtcatt cgttaaaatc aagaggccct
      661 ctgtgtcttt atctaatcgg ccgactggaa aaagatcttc ccgataatcc tggtcttcca
      721 ataaatcgat tacggtttga tcatgattat cctgagtagc agaaatgact cctgcaggtt
      781 tatgcaaaat gtagtaaaaa tctttttgat acgtaatgat ttctcctaaa taagtaaccg
      841 catcttcgaa ttcatttact tgcgctttat cgcttttcac cacttgtcca ttgacttcga
      901 tcgttccttt tttgattaat cctttgactt ctttgcggct gccaagtccc atatctgcta
      961 aaaacttatc taaacgcatg cgtttccccc atttcaaaag agcatcaatc tagctcttct
     1021 gattcactta ttttattttt aatttatgtc gtaaacgcgc tattcctggt cctagtagtc
     1081 tttctgccaa gcggattttc aaagctaaat aaccatacac taaagcccct atacctgcta
     1141 ctacaagaat caacaataga gactggaatt tgctatcttc gtttaaaaaa gcaccaaaaa
     1201 tcatctttgt gatccacgaa gccaaaagca taatcaaggt aagaataaag atcagcaatg
     1261 tcctgcgcca gacaaaactt ggtttaaagc gggctacttc atggattctc tttaagatca
     1321 aggtacaaga cacagcaaat ccgatcattg tcgccaataa agggccgtat acttcaaata
     1381 aacgaatggc tgggtattga agaaggagtt tcactagaaa cccaataacc aaataattaa
     1441 ttgccgcctt gttttcaaac attccttgca gcatgttgga aactagcata tatagtccta
     1501 ggaagagtcc tacaaaactt gcttggatca gtacattact tcctagagaa tccggtgtat
     1561 aaaataatgt atttagcgga taggcaagta acataacacc aaatgtcgcc ggaaacataa
     1621 aaaatgaaaa caactgtaaa tttccgcttg tcaattttgc cagacctact cgatctttca
     1681 acgtaacagc ttctgtaatc aacggtaacc cagtagacgc gatcgatgtt gccaatgcaa
     1741 taactaccat cgtcaactta tctggattgg cactaaaaat cgaaaaaaga tccagtaact
     1801 gtgcattgga atactcggtg gatgcagaca tgatcttcat aaaagtgaac tgatcgacta
     1861 atttgaagat cgttacccct gaacccacaa taataaacgg aatcgcttct ttaacggtat
     1921 ctaacaacaa ttccttcgtt gcaatattta ttttgttttc gctgtagtga ataaacgccg
     1981 atgtatacgc ttgatgtttc ttcaaaaaat acaaaaggac aagaatactt gctagcatac
     2041 cgataaatgc cgcgaaagtt gattgcgtga ccgccgtcac atagtcacca tccatgactt
     2101 tcatgatgat gaatgtagcc aatagcatat aaaaaatacg tgccacctgt tctacaatct
     2161 gcgacagcgc ataaggcatc atttcttgat tgccttgcaa ataccctcgt atcacactca
     2221 tacaaggaaa aaccaataca gcgatactca aagaacgcat cgttggaatc agttcttctc
     2281 cgccaccaga agcatgagct agccaaggag aggcgatgta catgaacaaa gcaaacaaca
     2341 ggcctaaccc gcccatcatc tgtaatgcac ggataaataa tcgtcgtgaa gtgccatatt
     2401 cattcaatga attatagcgt gcagtttgtt ttgcaatcgc agcaggtatt cctgctgtag
     2461 agatcagtag aaacaatgca tatatattgt atcccatatt gaataatcca ttggcagcct
     2521 tagcattttc gcccatccaa gcataccagg gaatgatata gacagcgcct agcaatcttg
     2581 aaatgatatt actggcggtc atccaagcag agcctcgtgc cattttttct tgcggtgtta
     2641 attgatctaa tgctggtttt ctttgattat ccatcggtca gattccaact ttctacaaaa
     2701 cattactatc attttaattg gtaaaacaga aactggcaat cgattttata taacttttat
     2761 aagaacaatt gtttttttga attcggtttc gtttttttgg taaagtaaag aaatatacag
     2821 ccttttatgg ctaacattta taggagatat caatgacaca agaaataaaa aaactacccg
     2881 ttatattttt aggtcttttc tttatcacag ttggattgaa ttggttcttg ctccctcacg
     2941 atatagcttc cgccggagtg ggcgccatcg gacatttagt tgaaacggcc ttttctatcc
     3001 aacgctcagt tactgtttgg agcatcaatc ttatgatgct gcttctagcc gctgtcctat
     3061 taggaaaatc agtatttttt aagactgtag cgggaagtct gctttttcct gttgttttag
     3121 agattgttcc aaaagtggaa atgatctcct cacatttctt ttcactgatt tcaggaagtc
     3181 ttttgttcag tcttggggtt tacacattgt acacagtcgg ggcctcaaat ggcggtataa
     3241 cgataccgcc gattattttc cacaaatttt ttcggcttcc aatgccgcaa ggacttttac
     3301 ttaccaatag tcttatcgtc tttttgaatt attatgtttt tggattgttg gagacactat
     3361 tcgttctcct atctattaca gtcagttcat tgtttatgaa aatactcatc cgcttgtccc
     3421 ctgtgtcaag aaaatttgaa taaagctgga ttttgtcata gcggaaacaa acgatatatt
     3481 aaaaagagtg tgctataata aaccaagaaa ttgaataaag cgagggatca atcaatgagc
     3541 ttctctttag aagacatccg tcatttacta ttaaaagaaa atctattaaa agaatttgtt
     3601 tcaacaagag gctggcatct tgatgtacca gaaaaagctg aatttcttca gttaagttat
     3661 gattcaagaa aagctgatgc ttctactttg tttttctgta aaggaatgaa tttcaaagaa
     3721 gaatatttgg attcagcaat cgaacagggt attcaatatt atgtatcaga acaaccatac
     3781 gaaaattcag cagtaggaat catcgtaacg gatatccgta aagcaatggc gcttcttgct
     3841 atggcttttt acgactatcc gcaaaataaa ttgaaagtga tcggattcac tggaacaaaa
     3901 ggtaaaacaa ctgctgcgta ctttacaaaa gcaatcttgg atcacacaac aaataaaaaa
     3961 acagcattgc tttctactat gaatacaacg ttagatggca aaacatattt caaatcccat
     4021 ttgacaacgc ctgagtcatt agatttatat cgtatgatgt cagaagcagt tgaaaatggt
     4081 atgactcatc tagtgatgga agtttcttca caagcttaca aaacacaacg cgtatacggc
     4141 ttgactttag atgtcggaat cttcttgaat atctcgcctg accatatcag tccgatcgaa
     4201 catccgacat ttgatgacta tttctattgc aagcgacaat tgatcttgaa ttcaaaaaca
     4261 gttgttttaa atcatgaaag tgactatttt gatttactta gggaaacagc cgatctattc
     4321 cagattccaa cgatcactta tggtcgttcg gaagaaagca attatcaagt gatccgttca
     4381 gataaaggta ctcacggttt tactttatct tctcatgaag atcgcttagc tatctgcgat
     4441 actgcttatg atattttgct ggctggagga ttcaaccaag aaaatgcagc cagtgcgatc
     4501 attgccgcag cactaacagg tgcctccagt caagatgcac aagacggatt aaaagaagct
     4561 cgtgtaccag gccgaatgga tcaattgatc caatccaatg gagcgcatgt ctatgtggac
     4621 tatgcacata attatctaag cttgaaaaca ttgctgaaat ttgccaaaaa tgaacatcca
     4681 gatggccgtg taattgttgt attaggcagc ccaggaaaca aagcaatctc ccgtcgacat
     4741 gattttggaa aagtgctatc agaaacagct gacatcgcct ttttgacggc agatgaccca
     4801 gcatttgaag atccgaaaaa aatcgcggaa gaaatcaatg aagcaattac taatccagat
     4861 ttgatcgttc attatgaaat ggatcgtcct gaagcgattc gacgcgcttt agcagaaagc
     4921 gctccgcaag attcagtcgt tattgcggga aaaggcgtgg atccctatca aaaaatcaat
     4981 ggtgtagatg aaccttatga aggggattat gcaatcgtga agcggttgat tgaagaataa
     5041 tggtatttat atactagtgg tttcccattt ctaagaaaaa aagggactgt gattttttgt
     5101 cacagttcct tttttcttag aaatcatttt aacttttcac tagctaattc tgctgccttt
     5161 tctctttctt catttaataa tttattatca tagaatttga taaatgggaa ccagataatg
     5221 aatgcgatga ttgcacaaat gactgctaac acagcagctt tccaatcacc accactacca
     5281 acaaatgctc cgatcccgac tggtgaaggc cacggcatct gtgcaagcat cggtttgacg
     5341 atttccagcc gaatggcaaa ataagcgagt gtagcagaag ccatcggagc taagaaaaac
     5401 ggaaccgcta aatagggatt gtagacgatt ggcatcccga aaatgattgg ttcattgata
     5461 ttgaataatc ctggtacaag tgaggctttt cctaacactt tcagctgatc agatttcgcg
     5521 aggaatgcaa taaagatgat caatcctaaa gtcgcacctg atccaccaac ggttacataa
     5581 gaattgttga attctcctgc caacggataa tttgcgcctt gtgcgttagc tgccatatta
     5641 gctaatacga tcggggtcaa gaaagaagtg atgatattcg ctccatggat ccctacgatc
     5701 cacaatgcat ggatcaagaa ataaatcacc atgacaccaa gccatgtatt cgtcaactgc
     5761 gtaacaaacc caaatgggat agcaatcatt ttgaagatat ccgttcctag tgcaactaat
     5821 aatccattga tgatcatcac cacaaacgca atgacaaatg ttgggatcag agcagtgaaa
     5881 gaacgagaaa ctcctggcgg aacaacatct ggcattttta tgacccaatt tctcttgaca
     5941 cacatacaat acaattcgac cgcaataatc gacataataa tagctgtaaa gattcctgaa
     6001 gttcctaaac gttcgacaaa agagcccatc ctaataccat ttactacagt ttctgtctca
     6061 ttgatagaag caatcaagct gattttacca tcagtcatga tcaattcagg gatacacata
     6121 aagaatgcaa aaacagatag taatgccccc gtgagcggat taacatttaa tttttgttca
     6181 tctcgttcaa ttgacgttaa ttcataagca aacacaatgg caaagtaaat agataaaatc
     6241 cccatcgtca ttgtattgac gatcatgtac aagtcactaa ctttgaaaaa agttgcatta
     6301 aaaaatcctt ctaaaaatgg gaacggtaat ggcaaaatat ttaaaaccaa aaacatcgag
     6361 cctacaatcg tgaaaggaat agaagccatc cccgctgcca tgacagcccg aacgatttta
     6421 agttgcgcaa ttttccccat aggtcccatc aggtatttct gcaaaaaatc aaacattagt
     6481 tattctcctt tcctaaatat aacgcttttt catattgttt aatacggata tactgttggt
     6541 cttggttgag tgaaattttc tttaaacgat actgtataaa tgcactggca aaaataccgc
     6601 ttacaacaag aatgaaaaca aataactgtc ctccattgtt atttgccata aaaggataca
     6661 agaatgtaaa tcctgaggta aacaacactg gaatcactaa taaattaaag acactctgga
     6721 acaaaaaata ccactttgtc catggaacgt tcgtttggtg ttttcgataa agctttactt
     6781 gttctcctac tgcaggcaaa atagctaaaa tcagaatcat cggtaaacta cttcctaaac
     6841 ttttagctga tagaagcaga atcatccatt gcatgttcat gaaaaagaaa aaggcagtca
     6901 ggtaacgtac gattagataa cgattgaaat acatgttttt caaactaagt tgcttttttt
     6961 ctaattcgat tttccttttt actgtatcct gcacaatgat ccttcctttc tatttatttt
     7021 tttgatacaa ataattcatt tcctgtgcaa cttccaataa tgtcatcgtc gtcatcagat
     7081 gatcttgtgc atggaccatg ataatttcca tttctatttt tgtcccgctg gcatattctt
     7141 tcagaagttg agtttgtgac tgatgcgcat gaagaatctc ttcatttgct tgtcgcaact
     7201 tctcttctac ttcttcaaac atctctttac gcatcgagcg aaatgcttca tgtatcagtg
     7261 tccgtgcatt tccgctatgg aaaatgatat caaaagaagt cacttgtagt gtttcactgt
     7321 ttattttttc agccatctgt tttcctcctt attcgatcag tttgataaat ttactgctga
     7381 aattctcaaa aataggttca gctaagattt cctgctgtac ttttagctgg tcaactaacc
     7441 taacgattgc tttagtcacg accgtaattc cttcattttc gacataagaa ggcgaaacta
     7501 aaaaaacaaa atgaatttct tgatattgat cccactgcat cccagaagga atcaatgcaa
     7561 cggctacctt tgtttcgatt ccaactggaa cagcgggatg tgggactgcc actgtatcac
     7621 tgaatatgat ttgtcccata ttttcacgtt gctggatctg ctttcgcatt tctcttatat
     7681 atccttcttc ttcatttatg ctcagctttt gcagcaaatc atgaagaatt tcttcctttt
     7741 tcggttcgta cgtataagtc ttgaaaaatt tttcagacaa ctgttcggtg taaatctgct
     7801 ttttttgctg tagagaaaat gctggtatcg gtctatctga ggaatgatag ggtatagatt
     7861 ctccaactac ctttcgtatt ttacgaacat catcttcatt caaaaaaata ctgacatgta
     7921 aaactggaat tttgaaaaac attgtcgaaa gatcaataga agaaataatc aggtcgattc
     7981 cttttaaagt tgtttcattg atctcataat agccttttac acttaccact gtaatatttt
     8041 taccgaattc actgactacg cgattcttta atagttgggc actgccgtaa cccgttgcac
     8101 agataatcaa tgcatgtagt ttatgcgcag ctctttcttt ttccaatgcc gccatgagat
     8161 gcaaagccaa ataggcccat tcatcttcat tgatggtata gtttttcaaa gccggcatgt
     8221 tgctgaaatg cctttttact gcgtcaaaga ctttgggatt ttctttcttt atctcctcag
     8281 tcaatgggtt ctcaatgcga atacctcggc tcaatcgaat catcatcggt gacaaatgat
     8341 cgagtagtcc attgaaaagc tggagatcct ctactaatga aaaatgaaat tcctgagaca
     8401 atcttttcag aactttcgct aattcctttg atagttcctg atcattttga ttttctttgt
     8461 gattcgactt agccatgaga tgtaatgtca aataagcaat ttcttcttcg ggaaacgata
     8521 cacctgcgat tacctcaata cgttgaacga tccgctgcgc cacttgatac tctactcgct
     8581 tagaaacttc ttgttcgatg cctaattcct tgatttcaaa cccttctcgg agccgtttga
     8641 tccccaaagc caagtgtaaa gtcagattct gtatgataaa atccgaaact tttaacttag
     8701 cttctcttgt ttcatcgaga atgataatga ctagctcttc aaaactgatg tctgggaaaa
     8761 aacggctttg ccctaaatat tctttcaatg aatttccata atggtttcca aaaaaagtat
     8821 ccatgataaa atgccgtcga tttctttctt ccccttcgat ccatgtccct tgaccatgct
     8881 tagaaacgat ttttagtgaa tatggaatca atcgctcctt gatttcttgc atgtcctttg
     8941 tcaaactgga acgactgata taaagctctt ctgccaaatc atccataaaa agaatggcat
     9001 cttccaaaag caattggttc aatatatact tttgtcgatc ttcggactca taaaatcctt
     9061 gcggttctct agcatatcca ggtactattt cttgccgact caaaaacaag tcaaatgtca
     9121 gtctttgtaa aatatgcagt tggtagccat aacctggttt agcaatgatc ttacctccgt
     9181 ttttttctac taactctttg atccgctgca agtaattacg gattgtccga tcagataata
     9241 acaattcaga agctaaacgt tgactggtga catactcctt cttgtgatca atcaaatatc
     9301 tcaacagcaa tttttccttc tgtttcatta tcatccactc ctattccatc atatgttctc
     9361 ttcgatcacc tctgctaact tctggatgcc tgtagggatt gggacatacg cttggaaagg
     9421 gatactaaca acaggctttc caacagattc tcctaatttt ttgaatttat ccaaaaacat
     9481 cgtcgtttgc gggctgatca gaaataaatc aaaatcacta ttcttaatca tattatcgcc
     9541 ttctgtagct gaaactgcat ctacgagtac ctcttctctt ttttgcttaa agaaatccgt
     9601 tgtctttgag gccatcatcg aggatgacat tcctgccgca caaataatca atgctttttt
     9661 cataaatcaa tcactccttt tatttttata aaccaagcat atcttgtaag cgttttctat
     9721 tcaaaatata tatttccgta gcattgcgga aaaaatgtgt atcatatctt gaataataat
     9781 ttctggcacc gtgatatact agtgatagaa ttttttcagg aggggattta gtgagtatca
     9841 cagtgaatat tttgtataca agtacaaatg gacaagccag aaaatttgca gaagaaatgg
     9901 tcgaaagaaa cattgtcaaa gcgattcgtg cagaagaagg aaacgagaaa tatgactatt
     9961 actttccact agaagatcca gaatctcttt tactgatcga ccgttggaaa gatgaagaag
    10021 caatcgaaaa gcatcacaaa tccgaaatga tggctcaaat aaacgaactc cgtaaaaagt
    10081 ataaattaaa aatgaaagtc gaacgtttca ctgatttacc gtaaaaagat acacatggaa
    10141 tgatagcaat ttatcatcca tgtgtatctt cactttttcc ttatttttta ttcgatctca
    10201 tctaatgatt caaaccgtgt ttttctctta tttttcggat aagtatttca gtcaccccaa
    10261 tatacatgat tcctaaacat accccaagga tattctcaaa aatgcggatc gttactgctc
    10321 ctggaacccc gaataaactg gcagtgattg ccaaagcacc aaaagaatta aaaactgttt
    10381 tatatctata ggtagaacaa acacctaaag ctagaccacc taatataccg acccagttaa
    10441 aaggaataaa ttggctgata ccaataaata aaacacatcc aatcaatgag ccaatgattc
    10501 ggtctactgc tcgttctttg aaaccaatcg acatcaatcc atagctagat acaatagaag
    10561 aaaatgcaaa ccctgcccac atcaatcgct gaaaagggac atactctcct gcaacgatca
    10621 aaagactaat gccaaatgca tagatgagca tccaaatttt ttcctgcgaa tacatgcctt
    10681 tcaaaaaaat ctctttaaac aagcttttgt ctttgttctt ttctcgatgt tttctgtaaa
    10741 ggagaactga aaaccaacaa aaaaacaaaa ataataagct acttgtttgt gtaaagaaat
    10801 tcttgttcaa cgaatcttta ggtaacgaat aaacaataaa gagatatgaa aagccgtaca
    10861 aagaagcatt ccccattttg ggatcactag cggttgccat taatatgctg cttagtaaaa
    10921 taaaatgaat caacatgccc ataactgacc atttgatcag ctggacatac ggtgctacta
    10981 atagtgagaa catgacgatt gctaaaccta tgatcgaatg ggaaatctta taaccaaagt
    11041 caacaaaacg aatactcatc aacatacaga ataaaaccac agcgtatggt ttatgcttcg
    11101 attcaaataa tgttgtaatt ataatgataa aacaaatggc aaacaatacg atgaataatg
    11161 atcgtaaaaa caatgaacaa atatacatgc acttttcttt tttagaagct gcttctcgta
    11221 ttttttgttt taaaataaac ggatctagtt gtagagactg ataaaaatcc acctgttgtc
    11281 gtcctttctt tttactttta ttttttacag tcatctatga caggttgtaa atactatgga
    11341 acatcatatg gcgttgtcat tttcttgtca actatttttc ctattcatgt ttttataaaa
    11401 aaacatatcg ttttcttgta tgattaaatc accacaaaat aaacaactga gagaacgata
    11461 tgctttatga atggttctat caaaaaaatt ctgagatcaa tagacaaaaa tttgatgatt
    11521 acagaggtct cttacgaaac cttacagaag aaaaaaacat tgatcgttgc tgcagttctc
    11581 ttccctgctc catgtgtctg tagaaattgt ggttctgctg tggtagatgg aaataggaaa
    11641 ttaatggtgg tgaaaaacaa gaagaaggaa accattgtcc actttgaata atataatcat
    11701 atgccaatgg ttatacgcct aaaaaagcag cgctatacct gtaaaaactg tcgaagccat
    11761 tggaacgctc aaagttattt tatccgtcca aggcactcga tttcaaatca tgtcagacat
    11821 aaaatcactt ctttactgac tgaaaaagtg tcgttatttt ttatttcgaa aagttgttag
    11881 gtatctttga caaccgttat tcgcacattg aaagagttga gaagctattt accaaagtct
    11941 tccaaaagaa ttctgtcttg agtgttgagg gttgtgaatt tcgttctcac gtttccgttg
    12001 aggataaact gagttttatt tccgcggatg gcgagacaga aaaattaatc gagaaaattt
    12061 acctacacaa aaaattcctc gattaacaaa ctatttcctt agctgcacta atcctaaaga
    12121 aatagaattc ttagttacag acatgaacgc cacctacttt cagctcacca aacatgttct
    12181 accaaatgcg aaattggtga ttgatcgatt acttggtttt tccacgcctt tcaaggaggc
    12241 gtaaccttta ttcacgagtt ggttgaagct ttccgagaca aaagacccga cttgtttttc
    12301 tccttattga caaagagcca ttaaaactag agaaaaaatt acaactgatc gctgattatt
    12361 tgtagaaaga aaatcaaagt attacccaat aaacttgagt cattaggcaa aaaacactgc
    12421 gaaactcaag aatgattttc gcagtgtttt tgtatatttt ttattaacta gtattactac
    12481 aaaggtttat ttttcacttc tcaacgcagc acaaaattgt ctttacttat ttactggttg
    12541 ggttcaacct tctctactcc gatactaata tttgtttgta taccccttct aaaatcggta
    12601 gtttccattc aattgatggg tactagttga ttttaaaaac tatgcttatt caatttttta
    12661 ttttagctca tatatttcgt ttctttctaa attttatact gatgaaaaca agtagcacac
    12721 ccaatattga caatataaac tggctaatga tggtttctcc agtttttgga agatgcttat
    12781 ttccactgct tgttggattg tttcctctta tatctgagct atcagcaact tttctattct
    12841 cttttgttaa aaagatcgcc acttcttgtt ctttttcgat cttgaaagtt actggtgtcg
    12901 catc
//
LOCUS       DACEUZ010000080        12868 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.080, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000080 DACEUZ010000000
VERSION     DACEUZ010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12868
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.080"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            200..634
                     /locus_tag="IXG44_001839"
     CDS             200..634
                     /locus_tag="IXG44_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ1658558.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            735..1826
                     /locus_tag="IXG44_001840"
     CDS             735..1826
                     /locus_tag="IXG44_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HAQ1658559.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            1856..2533
                     /locus_tag="IXG44_001841"
     CDS             1856..2533
                     /locus_tag="IXG44_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HAQ1658560.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            2865..4160
                     /locus_tag="IXG44_001842"
     CDS             2865..4160
                     /locus_tag="IXG44_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ1658561.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            4150..5610
                     /locus_tag="IXG44_001843"
     CDS             4150..5610
                     /locus_tag="IXG44_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ1658562.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            5746..7242
                     /gene="glpK"
                     /locus_tag="IXG44_001844"
     CDS             5746..7242
                     /gene="glpK"
                     /locus_tag="IXG44_001844"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HAQ1658563.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            7256..9079
                     /gene="glpO"
                     /locus_tag="IXG44_001845"
     CDS             7256..9079
                     /gene="glpO"
                     /locus_tag="IXG44_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HAQ1658564.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            9080..9796
                     /locus_tag="IXG44_001846"
     CDS             9080..9796
                     /locus_tag="IXG44_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAQ1658565.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            10102..12032
                     /locus_tag="IXG44_001847"
                     /pseudo
     CDS             10102..12032
                     /locus_tag="IXG44_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            12158..12712
                     /locus_tag="IXG44_001848"
     CDS             12158..12712
                     /locus_tag="IXG44_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HAQ1658566.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
BASE COUNT         4311 a         2137 c         2913 g         3507 t
ORIGIN      
        1 atactcaaaa ctaaacgact ttttcacaac ctctgattca cggtgttcca gaaggagttt
       61 tgtcgaaaac aagccgaaat tctttgaaga tggctacttc tttttttcaa atctagacag
      121 aggaggcgga cattattccg tgttttttga caaaaaataa aaatcatact atgatatgta
      181 cgccaatgga ggggatgaaa tggaaaagcc aattgaaaaa gcgttagatg aatgtctttt
      241 ctttagcgtg aaaaagctag atcgcatgtt gaataaatta gctgatgaag cgtttagaag
      301 gacaggtctg gcaccaactt atggttttat tttattgata ttaaaagaaa aagatggaat
      361 accacaaaaa gatattgcac aaatgctcta ttccgcccca tcaacgattg ctcgttttgt
      421 tgaaaaatta gaatataaag gatacgtcaa aacagttagc gaaggtcgct tgtcattagt
      481 ttatcttaca gaacaaggac gccaatttgc taaggaagtc gatggtagct gggaagaact
      541 tcatcagtca tacaaagcag ttttgggaaa tgaagcaagt aaagaattag cccagacaat
      601 gaatgatgca acagataagt tacaaaaaaa ataaaatttt ttaagcaaat tagttgcatg
      661 tacaactaat cgagtggttt aatgatagat gattctcgat aggttaatca gagcgaatta
      721 tgggaggaat gacaatgtca caatttgatc aatcaattac tttaccttca ggagttcaat
      781 tgaagaaccg attgatgctg tcgcctatga cgacacaact cagctttttc aatggagtga
      841 tcaccgaaga tgagatcact tattataaac aacgttctaa aggtcttggt gctgttatta
      901 ctggtgctgc aaatgtccaa gatatcggaa aaggctggcc aggtgaactc agcatcgctc
      961 atgatgaaat gattcctcga ttaagtgagc tagcgaaagc aatccaagga gaaggcgcaa
     1021 aagcgattat tcagattttc cacggtggac gaatgacaag tcgagcaact ttatctggag
     1081 aacagcctgt atctgcaagt gcagttgcag caaaacgtcc tgatgcggaa acaccacgag
     1141 caatgagtga agaagaaatc gttgaaacaa tccaagcatt tggcaaagcc acacgtagag
     1201 cgattgaatc aggatttgat ggaatcgagt tgcatggagc aaatacgtat ttgatccaac
     1261 aatttttctc tccgcattcg aatagacgag aggatcaatg gggcggtagc ttggaaaatc
     1321 gttatcgttt tattgaagaa ttattaacaa cggttttctc agcggttgaa caatatgcta
     1381 aaaaaccatt catcgtaggg tatcgtttct caccagaaga atatgaaaca ccaggtatcc
     1441 gttttgaaga cacgatctgg cttcttgaac gattacgtga gtcaaaacta gattatctgc
     1501 atgtttcatt aaatatgtat gatcgaatag ctcgttctga taaatacaac gataaatcga
     1561 ttcttgaagt ggtccataac acgattcaag gaaagatccc tttagttggt gtcggtggga
     1621 tccgcaatcg cgaagatgta gagcaggtat tgaaatatgc agaacttgca gcaattggac
     1681 agcaaatgat cgtcgatcct gactgggcag agaaattatt ggaaaaccgt gaggatgaat
     1741 ttgtaacgaa accttttgaa gaagcatata aagagcttta tttgccaagc ccattatata
     1801 atttcttgaa agcacgttat caataacgca atttttatag aaagaggatg ggaaaatgaa
     1861 agtattgatc gtattaacaa atgtagaaaa gtacgaaaag atcgagcgtc ctacaggttt
     1921 gtggttaagt gaactgactc atttttatga tgtattagta aaaaatggga tcgatgcaga
     1981 ttttgtcagt cctaaaggcg gatatgtacc tttagaaccg caaagtgttt tgaatatgga
     2041 tgaaatcgat tgggcctatt atgaagatga agatttcaga aatcgggcct tgggaaatac
     2101 cttacgtcct gatgatgtgg atgcaaatga ttatgcagcg gtttattatg ctggtggcca
     2161 cggaacaatg tgggattttc cagaagcaat agatcttggg aaagtcgctt cacaaatcta
     2221 tcgaaatgaa gggctggtct cagctgtttg tcacggtgtt gtaggattgt tgccagttac
     2281 agatgagtct ggaaaatcta ttttagctgg acgtacagtg actgggttca gtaatgaaga
     2341 agaggaagca aatggtacaa cgaatgaagt accattctta gcagaagatg ctttgaaaga
     2401 aaaaggagca aactatcagt ctggagcagc tttttcagaa gtggttcaaa ttgatgggcg
     2461 gcttcttaca gggcaaaatc ctcaatctgc tcatcaatta ggcgaagcag ttgttgaagc
     2521 attaaagaat taaaaataaa tgatgtgtct attctaacta ttacattaca cacgctaaaa
     2581 ctcgtctttt cgttcttttc tgtttcctat ttggacagag gtacgtaaaa ggcgagtttt
     2641 tttcttttat aagagtacat tttttgaaac atctcttcag aaatgatact cttcaattag
     2701 gtccactagt acattattga gaagcagacc tagaacacgg tatgatggag agggttgatt
     2761 ctgctgcatc gctatgtatc ataaggttga aagcgagtta tgaatactgt gttttctgat
     2821 tgagaagtta gcagtagaac accttataag aaagaggaat gcagatgaac attgtaattg
     2881 tcggcgggtc gtttgcaggg attcatgcgg ctatttatct tcgccaattg atgccaacaa
     2941 gtgaaatata tttgattgaa aagcaatcga agttaggatg gattcccagc gggttcaatt
     3001 tgatattaaa aggaaaggtt accagtgaaa aacaactttc ttggatcacg aaagaagaac
     3061 tgactgaccg ctatcgaatc catgtttaca cagaaaagac agtaatagga ttgaaagaaa
     3121 aaaaagtcgt tttagcagga ggaaaaaaat tggattttga tcgtttgatt ctagcgactg
     3181 gttcaaatca aaagtttcga aatatttctg cagaatcaac gcttattcac ccagttaaaa
     3241 agatacaaaa cgtaacggct cttcagcaaa agatcatgaa tgcaaagaaa attgcaatta
     3301 ttggtgcagg acaagcagga atcgaaatag ctgaagggtt ggcatcaaga aaaaaacaaa
     3361 tccatcttta cgaaagccga aagtcaattc tttttcgtta tctagatcct gaaatgacag
     3421 aaccgttagt aaaggaaatg aaaaatcaag ggctttcact ttttttagaa gaacaagttg
     3481 tgtctttgac cgaaaatgaa accgctattg tagagacgga aaaaagaaag gagaactatg
     3541 atcttgtttt gctggctaat cattctcgtc ccgacaatca gatgtgggaa gaacaattaa
     3601 cattgaatga tgatggaacg atctgggtag atgactactt gcaaacgtct caaaaagacg
     3661 tttatgcgat tggcgatgcg atccaagtaa cttttcgtcc aacaaatgaa aaaatgtatg
     3721 tttctctagt aaataacgct gtccgtacag cacgaaatgt cagcaagaca attagtggct
     3781 cacagacaaa ggatcaggga acctatcgtc cagtcggcaa tcagtggttt ggctatttct
     3841 taggaagcgt aggtttgaca gaagaagaga gcatttttta tccccaaaaa attgaaacag
     3901 gatacgtaga ctacaagctt acagcaataa atcaaagcaa agttaaactc aaatttctat
     3961 ttgattctga aaaaaagatt cttggagcac agctccaaag caaaaaagag atgttccacc
     4021 ttcttgacca actgacaatt gcagtagaag aaggatggac gtttgaacag ttagaagaac
     4081 atgagctatt tttccagcca gaataccgtg tgccagatat ggtgtggtca aaggtggcga
     4141 atactgccta tgaagattga agcattatta gaaagaaaag agcagttaca gatcctgatt
     4201 ttgcgaaacc ttgttctgca agggggaaca gcctctatca atggtttgag agagcatgtc
     4261 caactgtcaa aagcctcgtt tgatcaatat ttggaagata ttgaattgat tggccgaatg
     4321 atggaaaaaa aagtagaagt acagcgaaat gagtatcagg cgctgcttgt gttggacgaa
     4381 gatgtaagtt tggaaaaaat tctattgttt ttattgcagg aaagcttgaa gtttaagatg
     4441 ctcgtctatt tattagaaca tcagcaagta tccatcgttc gattagcgac agcgtttaat
     4501 atcagtgagt cttctgtttt tcgaaagatc aaagaattga accagctctt agaggaattt
     4561 ggattacaaa tcaaaaacgg tcaattatac ggagaagagc tacagatacg atatttttat
     4621 tatgaactat tccaatatat cccggaagat cagcgaccgc tttttttgca aaatacacca
     4681 gaaaaaagac cgttcattct aggattggac cgtgtattag aaacaacgtt tacggcatca
     4741 gcagaagcac agattgcttg ctggctcggg attacgaaaa aaagactatt gaatgaaaaa
     4801 agtacgtacg caactttgaa agaaaagaaa ttactctatc agtcggaccg gctttatcaa
     4861 gcaatcgatc caatcatcgt gatgcatctt agtcgaacag cagcagaact gaatgtatac
     4921 gaaacaatga tgttttacag cttttttgtt tctttttcga tagtagacga agaaatattt
     4981 tatcagtatg atttgacacg gagcaaaaag ttaccaaccg ctgttttaga tacttacatt
     5041 cgggaaacga tgttatggca ttatcgtcct cgtcgtttga aaatcaaaga agaaaaggca
     5101 gtaggctacc aaatttccca gatcaacaat gaatggtttt tctttgttgg aaagatcgaa
     5161 gtctatgagc gtgatcggct tttggaacag cagcagaaaa tgctcgggca ttccttaaca
     5221 cagttactgg cgaaattaca ggaaacagcg gtgcagcaat tgccgaggaa aagagctgaa
     5281 gatagtgagc ttagttacct gatgatccag tatgccaatg ttcttttgat gatcgatttt
     5341 tatattgcaa aaccagtagt gattggaatc gatttagaaa gtctgccgat ttatcgtatt
     5401 gcttttcaac aatatctgat cagagaactg aggggaatcg gcggtattga gattggaagt
     5461 tatgaagaag gtaaagaata tgatttggtg atcacatttt gccagagaaa caaaaaacag
     5521 tgtgaatatt atctgtcaga atttgcgtca ccttacgaca ttattcgatt gaaaagaaga
     5581 atagagacgc tgaaaaaaga aaagaattga ttaaaaaata gcttcataat aaaaaactgt
     5641 caaaaaaaga aaaaaagaat aaaattttga cagtttcttt ttttaaaaca gtagtatgat
     5701 aagagtagtt aaagaaagcg tttgcatata atggagggaa tcacaatgac agaatcaacg
     5761 tatatcatgg cgattgacca aggtacaact agctcacgag caattatctt tgacaagaaa
     5821 ggaagacaca tcggcagttc tcaaaaagag ttcacgcaat atttcccgca agaaagttgg
     5881 gtggaacatg atgcaaatga aatctggaat tctgtacagt cagttattgc cggagcgttc
     5941 atcgaatcag ggatcaaacc aaatcagatt gctgggattg gcataacgaa ccagcgtgaa
     6001 acaacagtaa tatgggaaaa agatacagga cgtccaatct atcatgcagt tgtttggcaa
     6061 tccagacagt cagctggaat tgcagataac ttaaaaaatg aaggctatca agaatttttc
     6121 cacgaaaaaa cagggttagt gatcgatgcc tatttctcag caacgaagat acgctggatt
     6181 cttgatcatg tagaaggcgc gcaagaacga gctgaaaaag gcgaattgat gtttggaacg
     6241 atcgattctt ggttagtatg gaaactgacc gatggacaag cacatgttac tgattactcg
     6301 aatgccagtc ggacgatgtt gttcaatatc catcagctag attgggatca ggagatttta
     6361 gatttactaa atattcctcg agtgatgtta ccaaaagtca cttctaactc agaagtttat
     6421 ggttatacgc aaggttatca tttttatgga agcgaagcac caatttcagg gatggcagga
     6481 gaccagcaag ccgcattatt cggacaaatg gcattcgaac caggcatggt caagaatact
     6541 tatggtacag gttcatttat tgtcatgaat accggtgaaa aaccgcaatt atccaaaaat
     6601 aatttattga cgacaattgg ttacggcata aatggaaagg tttactatgc tttagaagga
     6661 agtatttttg tggctggttc ttcgattcaa tggctgagag atggacttca aatgttgcaa
     6721 aaagctagcg attcagaaga cgcagcgaaa cgctctacaa gcgaagatga ggtttatgtt
     6781 gttcctgcat tcgttgggct aggtgctcct tattgggatc aggcagcacg aggggctatg
     6841 tttggcttga cacgtggtac gacaaaagaa gatatcatta aagctacttt acagtcgatt
     6901 gcttatcaag tgagagatat catcgatacg atgcaagatg acactggaat caagattcct
     6961 gtattgaaag tagatggcgg tgcagcaaac aatgaatatc tgatgcaatt ccaaacagat
     7021 atcttgaatg taccgatcca acgagcagaa aacttggaaa caacggcgct aggggcagca
     7081 tttttagcag gattagctgt cggttattgg aaagatacag acgagatccg cgaattttat
     7141 gaagctggca agctatttga agttcaaatg gaggaagagc gccgagaaaa actttacaac
     7201 ggttggaaaa aagcagtaaa agctacccaa gcatttgaat aaggaggaaa tccatatgtt
     7261 ttcaaacaag acaagacaag atagcattca aaaaatgcag caagaagaat tggatctgtt
     7321 gatcatcggt ggcggaatca ctggtgccgg tgtggcagtc cagtcagcag catcaggaat
     7381 caaaacagga ttgatcgaaa tgcaggattt tgcagaaggg acttcctctc gctcgaccaa
     7441 acttgttcat ggcggtattc gttatctgaa gacatttgat gtggaagtag tagctgacac
     7501 agttggtgaa cgtgcagtcg tacaaagtat tgccccacac atcccaaaac cagatccaat
     7561 gcttttgcca atctatgagg gcgaaggagc aacaaccttc aatatgttct cggtcaaagt
     7621 agcaatggac ctttatgaca aactggcaaa tgtgacagga accaaatatg agaattatac
     7681 gctgacacca gaagaagtat tggaaagaga accgtttttg aaaaaagaag ggctaaaagg
     7741 cgcgggtgtc tatctagatt tccgcaacaa tgatgcccgt ttagtgattg ataatatcaa
     7801 aaaggctgcc gaagatgggg cttatctagt aagtaaaatg aaagcggttg gctttttata
     7861 tgatggcgat caaatcgttg gcgtcaaagc tcgtgacctg ctgacagatg aagtgatcga
     7921 aatcaaagca aaattagtga tcaatacgag tggtccttgg gtagataaag tacgaaactt
     7981 gaattttacg cgtccagtct ctcctaaaat gcgtccaact aaagggatcc atttagttgt
     8041 agatgcgaaa aaactgcctg taccgcaacc cacatacttc gatacaggaa agcaagatgg
     8101 gcggatggtt tttgctattc caagagaaaa caagacttac tttggtacga cagatacgga
     8161 ttaccaagga gattttacgg atccgaaagt cacacaagaa gatgtagatt atctattgga
     8221 tgtgatcaat catcgctatc cagaagcaaa tatcacattg aatgatatcg aagcaagctg
     8281 ggcaggactt cgtccactgt tgattggtaa ttctggttct gattacaatg gtggagataa
     8341 tggatcgatt tcagacaaga gcttcaataa agttgtcgat acagtaagtg aatataagga
     8401 aaacaaagtt tctcgtgctg aagtagaaga cgtgttgaac catttggaaa acagccgtga
     8461 tgaaaaagca ccttctacga tttccagagg tagttcttta gaaagagaac cagatggttt
     8521 gctgacttta tctggtggga aaatcaccga ttaccgtaag atggcagaag gagctttgcg
     8581 attgatacgc caattattaa aagaagaata cggaatagag acgaaagaaa tcgactctaa
     8641 aaaatatcag atttcaggtg gaaacttcga tccaactaaa ttagaagaaa cagtgacaga
     8701 attagcaaaa gaaggagtag cagccggttt agaggaagaa gatgctactt atatcgctga
     8761 tttttacggg actaatgccc gacgtatctt tgaattagca aaagaaatgg caccttatcc
     8821 tggtttgagt ctcgctgagt cagctcggtt acgttatggt ttagaagaag aaatggtttt
     8881 ggccccaggc gattatctca ttcgtcgtac aaatcatctg ttgtttgaac gagatcagct
     8941 tgatgagatc aagcaacctg tgatcgatgc aattgctgga tattttgaat ggacagaaga
     9001 ggagaaggcg caacagacta aacgtttaga agcattgatt gcagaatcag atctgcggga
     9061 actaaagggg gagaaataaa tgaacagtga tatgactcaa atcatggggg agtttattgg
     9121 gacactgatc ctagttttgc ttggagatgg tgtgtgtgcg gctgtgaatt taaacaaaag
     9181 taaagcacag gcttctggct ggattgtcat cgcatttggt tggggactcg ctgtaacaat
     9241 ggctgtctat atttctggat tcatgggccc agctcatttg aatcctgcag tctctctagc
     9301 tatggcgatg acaggggcaa ttagttggaa tcttgtcgtt cctttcatta ttgcgcaagt
     9361 tttaggagct tttgcagggg ccattcttgt ttggctttct tatttgccac attggaatgc
     9421 aacgaaagat gaaagcgcga ttttaggaac gtttgcaaca ggtcctgcta tacggaatta
     9481 tccagcaaat gtgatcacag aattaattgg tacatttgta ttagttcttg ggttgctagc
     9541 ttttggacaa aacgaatttg cacctggaac gaacgtattc gctgtaggtg gattgatctt
     9601 agccatcggt ctttcattag gagggccaac aggatatgca atcaaccctg ctcgggactt
     9661 tggtcctcga ttagcacatg cggtattgcc aatagctaat aaaggaactt cagattgggc
     9721 atattcatgg gtaccaatcg caggtccaat gatcggagca attatcgcgg taggtgtcta
     9781 ttcattaatg gtgtaaatct ataaaacata ctaaagtttg acaaccaaaa tgtgtcttgc
     9841 tatagttaag tggaaaataa ataagaccta gaagaatggg gtatgttttg tgagtgtagc
     9901 tggtatctaa tcagtaagtc gaatagccaa taaacaaagt ttatttggtg tgcttatgaa
     9961 acggtacctg tcaggaaaac aatatctttg tattctaata aggataggac atgacttttt
    10021 taagctgtgt tctatccttt tatttatctc taggccctta tttattttgg gacaggtatc
    10081 tcttcaagaa aaggagagaa tatggacaac gaaatgatta gtcaattaca atttgatcgg
    10141 atcaaaaagg aaatccaagc aagagcaatt ggaaactata gcaaaaaacg aatcagcgat
    10201 atgacggtct cgacaaacct acaaactgtc ttggatcgtc aagaagaaac aagagaagca
    10261 cgcttgatct tagaaagcag tcagcatgtt ccatttatgg ggttgccgag gatcgatgca
    10321 ctgacagaac aagtcaaaaa agggttgatt ctgcagccaa ccgatttgat cgaatatgcg
    10381 gattttctta gaagcagtcg gatgatcaca aagttttttg ataagaatca ataccagaca
    10441 cccttgttgt ttgcttatag caaacatctt cctgatttga taaatgtaga agagttgatt
    10501 gaccagaaaa taaaaaataa taaagtcagt gacgacgcgt cacgtaattt gcgtaaagta
    10561 cgcaaacaat tacaactaat cgaaaaagaa atccagtcaa aattactgaa attcttgcgt
    10621 catccgaaaa acaaagaaat gatccaagaa gcaatgattg ttcaaaaagg cgaatactat
    10681 acgattccta tcaaagccag ctataaaaat aaaatagatg gaacgattat cgatgaatca
    10741 aataaaagaa cgacggtttt tattgaacca actgtgattt ctaaattgaa tgaacactat
    10801 cagttgttaa aagctgaaga aatcagtgaa gaatatcaag tcctggcggc attgactggt
    10861 gctatcgcag aaaacgaaga agtgatcgat ttattgatcg aaacgatcac ggtgttagat
    10921 atcatctttg ctcgggcaaa atttagccga gaaatcaatg gaagcacacc tagaatcaat
    10981 aaatcggaac atatcattat caagcaaggc agacatccat ttttacctga gcatgcggta
    11041 ccattggatg ttgaaatcgg taaagactat cgaggcttga ttatcaccgg agcaaatgcc
    11101 ggaggcaaaa cagtcgtgtt gaagacagtc ggtttattga ctttgatggc gatgttcggt
    11161 atgcaagttc cggcaaaaga agggacagag cttgctgtct ttgatgagat tttcgtcgat
    11221 gtcggtgacc accaaaattt agaaaatgcg ttgagtacat tttctggtca tatgcaaaac
    11281 atcgctgcta ttttgaaaaa gataaaaagg aatacgttgg tacttttaga tgagattggc
    11341 agtggaacag agccaaatga aggggctgca ctagcgatcg caatcatgga atctatgtat
    11401 gagcaagggg cattgatcat tgccacgacc cattatggag aaatcaagaa attcgccgga
    11461 gatcatgaag attttgtgcc ggctgctatg gctttcgatc gggaagcact tcgtcctaaa
    11521 tatcagttgc gggttggaga aacaggagaa agccaagctt tgtggattgc tcataaaatg
    11581 gatatgtcga tgaaattgat ccatcaagca gagcgttact tggcggaaaa agcctatcgg
    11641 acacagaaaa aagaatttct tcctaagaca gcagttacaa ataggaagga gaataaaaaa
    11701 gaacgccagc tctttgcaaa aggtgatcgg atatttgtca atgaatacca caaaaagaag
    11761 cgttagttta tgaagatata ggtgaagata cgattgatgt atatttagac aaaaaaataa
    11821 tccacgtgcc tcgtcagcgt gttcgtctgg tgcgttcagc agaagatctg tacccgacag
    11881 gttacgattt agacagtctg tttatcgatt ataaaacacg taaacggcag cgtgacctag
    11941 agcgaggatc aaaaaaagct cataaagtac tagtaaaaga aatgcgcaaa cgacaggaag
    12001 aaagaagagt aaacgatgaa aacagcaagt gattatctca aattagctca taatatcagt
    12061 ggctgtaaag aaaaataaaa acagtgaaat cattggtttt attccccgtg taagctaaaa
    12121 taaaaagaaa agagtgctga ggaggtaggg cgataaaatg aaattaagca cagactggcg
    12181 taaagaaata caaacgattc cgaatctttt atcgatattc cgtatttttt tgctgccgat
    12241 ttatctttat tttgtgctga gacagtcatt ttatatagca ggagcggtca ttgttgtctc
    12301 aggtttgtct gattatctag acggggtcat tgctcggaga tataatcagg taacagactt
    12361 agggaaggtg cttgatccgt ttgcagataa attgacacaa ctttttctga ttttgtctat
    12421 ggcatggtat cgaccgtggc tttggctgtt gtttggtcta ttcttgataa aagaaggctt
    12481 tatgtttgta gctggattaa tcgggctatc taaaaatatc aaattaagtg gagcgaagtg
    12541 gtatggcaaa gtagcgactg ctgtcatcta tgttggcatg atccttttgc tgcttttccc
    12601 agaattacca acgctgtggg tgagagtcat ttttgccgtc atcacttacg gtcttttaca
    12661 atcttttgtc ttgtatgctg tcgagtaccg aaagatgttt caaagaaaat aaaaaagggg
    12721 tgtgacctat gtcgcattcc tttttttgat aaatagactc ccagtagcta cttctcgata
    12781 ataagccaac caaaccaaaa acaggagaag ttgtttttga aattatttct tgtgattcgg
    12841 agttaaatgg taggctcaca acctcttg
//
LOCUS       DACEUZ010000081        12670 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.081, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000081 DACEUZ010000000
VERSION     DACEUZ010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12670
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.081"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            6..305
                     /locus_tag="IXG44_001849"
     CDS             6..305
                     /locus_tag="IXG44_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1658567.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="IXG44_001850"
     CDS             378..1739
                     /locus_tag="IXG44_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAQ1658568.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="IXG44_001851"
     CDS             1761..2021
                     /locus_tag="IXG44_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1658569.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="IXG44_001852"
     CDS             2035..2787
                     /locus_tag="IXG44_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HAQ1658570.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="IXG44_001853"
     CDS             complement(2881..3486)
                     /locus_tag="IXG44_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ1658571.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="IXG44_001854"
     CDS             complement(3539..4513)
                     /locus_tag="IXG44_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HAQ1658572.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5670)
                     /locus_tag="IXG44_001855"
     CDS             complement(4891..5670)
                     /locus_tag="IXG44_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1658573.1"
                     /translation="MLLRVVGSIIFRYTLEEVNNVPKYEEIANILRERIRSKEYPLDS
                     FLPNQVDLVKEFGASRMTIKKAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEY
                     EGLSLQMKRRHRSLKSQIIHFDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVL
                     EHTFMPVHLVPGLKKEHLLASIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAE
                     NDPVLEVQQVVYLKDGQPIEYSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IXG44_001856"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IXG44_001856"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAQ1658574.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="IXG44_001857"
     CDS             7161..8558
                     /locus_tag="IXG44_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ1658575.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="IXG44_001858"
     CDS             8639..9079
                     /locus_tag="IXG44_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ1658576.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="IXG44_001859"
     CDS             9227..9553
                     /locus_tag="IXG44_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ1658577.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="IXG44_001860"
     CDS             9554..10303
                     /locus_tag="IXG44_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1658578.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="IXG44_001861"
     CDS             complement(10383..10838)
                     /locus_tag="IXG44_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAQ1658579.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11265..11540)
                     /locus_tag="IXG44_001862"
     CDS             complement(11265..11540)
                     /locus_tag="IXG44_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1658580.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            11834..12649
                     /gene="thiD"
                     /locus_tag="IXG44_001863"
     CDS             11834..12649
                     /gene="thiD"
                     /locus_tag="IXG44_001863"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HAQ1658581.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
BASE COUNT         4056 a         2175 c         2587 g         3852 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaaattta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaaa
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattcttttt tttcagtaaa cataaaggtt tgggtatcgt aaccttcttc atgtaggaaa
    11401 tagattattc ctttttctgt gttttgcgta taagctttta tactcctgtc ttttttgaaa
    11461 gaacagatcg tatagccatc tatttggtct ggtagtcgtt tgattttctt caaaatgact
    11521 tttttatcct caagtttcat ttttttcacc tgactttatt tttttcatat ggaaatcata
    11581 gcgcctttgg ttatatgaaa acaagaagga tactgaaata tatataaatc aaatagtggt
    11641 tatttattat tagaatagcg aatacaaata aacgaccact attttctgat ttattgcaaa
    11701 tgttttttca aataaatttg attaccaaga aaaggtagtg gtatatttac tgagggatac
    11761 tttgaaaaac atatgataga aacagtaaca gtattatgaa aaatcttttt ataaaaatgg
    11821 aggaatcgat ttgatggaag atcagtatgt acaagcaatg acgatcgcag gatcagatag
    11881 cgatggaagc gcagggatgc aagcagatat gcatacattc tttacaagaa atgtttacgg
    11941 tgtttctgtc atgaccgcat gtgttgcagg taattcttac ggaatcggag caagtgtgac
    12001 attgccaaca gattttatcg acaaagaatt tgaactgatt gcgaaggact ttcaaatacg
    12061 tgcagctaaa acaggtatgc tagcagattc aaagttgatt gagacagttg taaaaaatta
    12121 ccaaaaatat gattttggac cattagtcgt ggatcccgta atcgtaacga aacatggaaa
    12181 tcttttacta gaagaatctg ctttgcaaag cttgaaagaa aagcttgtcc ctttggccga
    12241 agtactgaca cctaattttt atgaagcaga aaagctgact ggacggacat tagcagctga
    12301 agaagatttt gtgcaagcag caaaagaact gcaagatatg ggtgcaaaaa acgttatgat
    12361 caaaggacat catgctaaaa atgcgcagca agaaacagtc aatgactacg tattgttcga
    12421 atcaggaaaa tcattttggt taaatgcacc attttatcct accgaccgga tcaacggaac
    12481 tggcgattcg ctatctgcat gtattgcagc tgagttagcg aaaggaaaaa cagttgaaca
    12541 agcgatccga ttggcaaaag actatgtaaa ccgtgcaatt cgtgaagaaa taaatgtagg
    12601 gcacaaatat ggccccatca atcattgggc agctggtgaa ttagagtaaa agaaagaagt
    12661 tgtgaaaaag
//
LOCUS       DACEUZ010000082        12594 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.082, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000082 DACEUZ010000000
VERSION     DACEUZ010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12594
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.082"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(68..1099)
                     /gene="queA"
                     /locus_tag="IXG44_001864"
     CDS             complement(68..1099)
                     /gene="queA"
                     /locus_tag="IXG44_001864"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HAQ1658582.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKIGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGKMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            1292..2467
                     /locus_tag="IXG44_001865"
     CDS             1292..2467
                     /locus_tag="IXG44_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ1658583.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESDTPEESKEV"
     gene            2471..3112
                     /locus_tag="IXG44_001866"
     CDS             2471..3112
                     /locus_tag="IXG44_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1658584.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            3109..4026
                     /locus_tag="IXG44_001867"
     CDS             3109..4026
                     /locus_tag="IXG44_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ1658585.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYDQTWFPSYGFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVNNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            4023..4694
                     /locus_tag="IXG44_001868"
     CDS             4023..4694
                     /locus_tag="IXG44_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1658586.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATDGTSIILAGALPTALMVIVTDWLLGILERRLDPA
                     SRTSR"
     gene            complement(4890..5724)
                     /locus_tag="IXG44_001869"
                     /pseudo
     CDS             complement(4890..5724)
                     /locus_tag="IXG44_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016619675.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5925..6191)
                     /locus_tag="IXG44_001870"
     CDS             complement(5925..6191)
                     /locus_tag="IXG44_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAQ1658587.1"
                     /translation="MSNYTVAIKNSATVDLRKIKQSNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(6191..6454)
                     /locus_tag="IXG44_001871"
     CDS             complement(6191..6454)
                     /locus_tag="IXG44_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAQ1658588.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSGFTNIDDIDWEAL"
     gene            6929..7114
                     /locus_tag="IXG44_001872"
     CDS             6929..7114
                     /locus_tag="IXG44_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ1658589.1"
                     /translation="MALNYGNKKWSLDEAEISTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(7256..9079)
                     /locus_tag="IXG44_001873"
     CDS             complement(7256..9079)
                     /locus_tag="IXG44_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HAQ1658590.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(9676..12381)
                     /locus_tag="IXG44_001874"
     CDS             complement(9676..12381)
                     /locus_tag="IXG44_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HAQ1658591.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPILQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
BASE COUNT         3623 a         2624 c         2375 g         3972 t
ORIGIN      
        1 ctttttcttt gtcataaaat ctttgtaaag cttttttgtt attcaaaact agcggctttt
       61 cataacctta tttcacgaac atcgcatctc caaaactaaa gaaacgatag cgctcttcaa
      121 tcgcatgctg gtaagcagat aaagtcaatt ctcttcctgc aaaggcacta actaacatga
      181 ctaaagtcga ttttggcaaa tggaaattag ttgagaaagc ttctaccact ttgaattcat
      241 agccaggcgt aatgaaaata tcagtccatc cgctgtctgc tttgatttct ccattaaatt
      301 tcgttccaat cgtttctaat gttcgaattg aagttgttcc gacagccacg attttcccgc
      361 catttttacg tacttggttc aatcgctcag ctgcttcttc cgtcaaacgg tagaattcac
      421 tatgcatatg gtgttcttca attttatcta cgctgacagg tcggaatgta cctaacccta
      481 catgtagtgt cagatagact aattcaacac cctttgcttc gatctcatca agtaattctt
      541 tcgtaaaatg caatccagct gttggcgctg cagcagaacc attttctttc gcatagacag
      601 tttgatagcg atctgggtct tccagacgtt ctttgatata tggaggcaat ggcatttttc
      661 ccaatgattc aagaatctct agaaaaattc cttcatactc aaagtcaatg atccgtccgc
      721 cgtgttccaa ttcttctttc actaccgctt gcaatctgcc atcaccaaaa gagattcttg
      781 tcccaacttt ggcacgtttc gcaggtttta tcaatgtctc ccacgtatct ccttgtgtat
      841 tcgttagaag aaggacttct aaatgcgcac cagtatctgg tttttctcca tataaacgag
      901 caggcagaac tcgtgtatta ttcataacta gcgcatcacc aggctgtaac tcttcaatga
      961 tttcatggaa atgtttatct tccatctctc caattttccg atccaaaaca agtaaacgag
     1021 aatgatcgcg gtcttttaat ggtgtttgtg cgatcaactc ttccggcaag tcaaaatcaa
     1081 aatcttctgt tgttaacata tcgtcactca ctttctatca tgtgcttcat cattctatca
     1141 tgtttttcat tctgttggca aaatcagcac tttttcactt ctattttttg ttttcctaaa
     1201 taaaaaaact accctatttg tattttttca ttgttttagg catactagta atagatagtt
     1261 cttcattgaa aatagaaaaa gaggtggcac catgattgaa tttcaaaatg tgtcaaaaat
     1321 ttataaaggt ggaaaagtcg ctgtagagga cgtcaatctt tcatttgaaa agggtgagtt
     1381 catttgtttt atcggtacga gcggaagcgg aaaaacgacg acgatgcgga tgatcaaccg
     1441 aatgactgat ccaacacaag ggaaaatttt gattgatgga aaatccatcc aagaaattga
     1501 tccagtggaa ttgcgacgtc agatcggcta tgttatccaa agtattggat tgatgcctca
     1561 catgacgatc cgtgaaaata tcacccttgt ccaaaaattg ttgaaagtca gtcaagaaga
     1621 acggaataaa acagctgaaa aaatgattga tcttgtggaa ttgccccgtg agatgttaga
     1681 tcgctaccca catgaattat ctggtgggca gcagcagcgt attggagtcg ttcgtgccct
     1741 tgcagcgaat caagatatca tcttgatgga cgagccattc ggtgcgcttg atcccatcac
     1801 tcgtgattct ttgcaagacc ttgtcaaaga tttacaagag cgcttgggaa aaacgatcgt
     1861 atttgtaacc catgacatgg atgaagcgat taagctagca agccgtatcg ccatcatgag
     1921 tgaaggccgt gtgatccaat ttgatacacc agaaaatatc ttacgtcatc ctgcaaacga
     1981 tttcgtagaa gagttgattg gagaagatcg cctactacaa gccaagccaa atgcgacaag
     2041 agtcggagaa gttatgctaa atacagcaat cacgattaca ccggaaaaat cattacaaga
     2101 agcaatcaaa ctgatgaggg aaaaacgtgt agatacgttg ttagttaccg ataattcgaa
     2161 tgtattaaaa ggatttatcg atgtagaaac gttgaataaa aagcgaggaa aagtatccag
     2221 tgtcggcgac atattaaata aacatgtttt ttacgtaaaa gaatccgcct atcttcgtga
     2281 taccttgcaa cgcattttaa aacgcggact gaaatacgtg ccggtcgttg atgaacagaa
     2341 caaagtcgta ggtatcctga ctcgtgcatc attagtggat atcgtctacg atgtgatttg
     2401 gggagaagat cccgcaacta ctgatgttgc agagtcagat actcctgaag aatctaagga
     2461 ggtctgacct atgcagcagt tttttcatca gcacggttca gaaattctct caaaaggttt
     2521 tgaacatctt tatatttcgg gacttgcttt gatcattggt atcatcattg ctgtaccgct
     2581 tggtgtctta ctgacacgtt ttccaaaagc tgccacgatt gtgattggct taaccagtgc
     2641 cttgcagaca atcccttctc ttgctttatt ggcattgatg atcccgctat ttggcgtagg
     2701 taaattacct gcgattatcg cactctttat ttattcatta ttgccgattt tacgtaatac
     2761 gtacattggg atgaaaaatg tcgattggaa ttatcgggat gtggcaaaag gcatggggat
     2821 gactgagttc caatccattt tttccgttga attgcccatt gctatgccaa cgatcatggc
     2881 tggtattcgt ttagctgccg tttacgtgat tgcatgggct acacttgcct cttatatcgg
     2941 cgctggtggt ttaggcgatt tgatttttag tggactgaat aattaccaac cagatctcat
     3001 tttggcaggt acgatccctg taacgatttt agcactattg gctgattggc ttctaggtct
     3061 cttggaacac cggctgacac cgatcgcact tagagaggag aatgaagaat gaaacgtata
     3121 ctaaaaatga ccatggttct tttctctctt cttatacttg cgagttgttc tttccctggt
     3181 cttgccagca atacagatga agacaccatt tcgatcacag gtggaatcac ttcagaagca
     3241 cagatcttag caagcctagt tgccggaatg gttgaacatt atacagataa aaataccgca
     3301 atcatcaaca atctggctac cacaactatc aaccatcaag cgatgatgaa cggagatgct
     3361 tcaatttcag cggctcgcta cacaggaaca gatttaacga ctacattgaa ccttcctcct
     3421 gaaaaagatc cgaaaaaagc gtttgctacc gtgaaagatg aatttgagaa aagatacgat
     3481 caaacgtggt tcccgtccta tggttttgaa aatacgtatg tctttcttgt tcgaaaagac
     3541 accgcacaaa aataccattt atctaaagtc agcgatctga aaaatgtagc ggatgaatta
     3601 gtagctggtg tagatacttc ttggatcaat cgtaaaggag acggttatga cggcttccaa
     3661 gaaacttatg gcttctcctt caattcgata ttacctatgc agattggact tgtttatgac
     3721 gcagtcgaag caggaaaaat ggatattgtt cttggctact ctacggatgg acggatcgcc
     3781 agttatgatc tagtcatgtt ggaagatgat cgtcaattct tcccaccata tgatgctgct
     3841 ccagttgtaa acaacaaaat attagctgat acgcctcatt taaaagaagc cttaaataag
     3901 ttaggtcata ccatcagtac ggaaaaaatg caggaattga actacgaagc agataataat
     3961 ctagtggaac catcagtagt agcacaacgt ttcttacaag aaaaccacta ttttgaaggg
     4021 aagtgaagca attggataaa atgaatgtct ttcaacaatt tttctattat ttccaggaaa
     4081 atggctccta tatctttgca caatttatcc gtcacttcct gatttctatc tatggtgtcc
     4141 tctttgcagc agttgtcggg attccggttg ggatcatgat ttctaggaga cgtaaattag
     4201 caaactgggt gattcgtttg gcgaatatca tccagacgat cccttctcta gccatgatct
     4261 ccattttaat cattggatta ggcctgggag tcaatgtcgt gattgttact gtatttttgt
     4321 attccctctt accgatcatc aagaatacgt ataccgggat gatccaagta gacaaaaaca
     4381 tccttgatgt cggcaaaggc atggggatga ctgctagaca acgactattc atggtagagt
     4441 taccactctc tgtttctgtt atcatggcag gcataagaaa cgcgttagtt gttgccatcg
     4501 ggataaccgc gatcggtgca ttcgtcggtg ctggtggtct tggagatatc attatccgag
     4561 gaaccaatgc aacagatggc acatcgatta ttttagcagg tgcattgcca actgcattaa
     4621 tggtgattgt cacggactgg cttttaggta ttttggaaag acggctagat ccagccagcc
     4681 gtacatcacg ctgatgttat tgtaaaaaac aaaagcttct gcgaccattt gttggtcgca
     4741 aaggcttttt tagttctgtc attttttaag gcaataatcc actctgattc ttccctcctt
     4801 atgggcaaag agttgctttt cttagctatt tatccattcc gtatactcca acttcatact
     4861 gccaacgggt tcatctctgc atccaccgac taagtttttt tcttatccgc taaggcattt
     4921 tgccaatcca ttttcccatc actcgtcatg gaattcatac cacatgatct ctaaatactc
     4981 gtctgcttgt tctttgaatt gggcatcaat tcttacatcc tttggcgtgg ctttcattta
     5041 agccttttcc atcagtcctc gattgttcga tgccttcagc tacatcatca aaactggtga
     5101 ctgccgtatc ggtaacttcg atcatctttt atacaatcgg gaacttgcca tcctcatcct
     5161 tggctaaaaa tatatctagc cattccagct tctcgccatg ggaggtttgc ggttcctaaa
     5221 accggtacat gattcttatg gactgcatcc gtcgcaacta gacttggcgg tacgatgatc
     5281 cttcaccaga agagcacccc cgtaaaccaa ttgatcgata tattgccaat aagaaaagac
     5341 attggcgtaa aagatatcga tccctcgtga tgcattgccg ttgatactgc tattcatggc
     5401 cgaaagcgcc acgactttga cttcttccgt ttgagtgaca ttcatgttcg gcaatgtcgc
     5461 tttggatacc cgctctgcta aagaaacgac actggtattg tatttcgtat ctgtatcttt
     5521 tccaaaagac catgccaacg aatcctctgg gaaccaataa gacgactccg gttgattgtc
     5581 cattgctttt ttcaacatac ttttggctgt ttctcgataa acgacatttc caattgtctt
     5641 cgcctcactt tggcgataat cgatggtcgt tgccactgcc aacactaaca acgatgctcc
     5701 aattttactg attttccttg tcacacttct tcctccttgt tttacataaa cccgtcaaca
     5761 ttccattcct aaactcaaac aatgaccctt caaaggtgga gatataggaa caaattaaag
     5821 aaatcatgca ataaatccaa gaaggttggt tttagaacaa aaaaagagtc tcctttttgg
     5881 ggagactctt tgttaactat ctaaaaaggt tggctcttct cacttcactc ataatgtgtc
     5941 caggcagaat aaatttccac aactttattt tcctcatcaa ctttgtaaac cactcgatgc
     6001 tgtctattca gcctgcgtga gtatctgcct tcatgtgtag gtctcagctt ctcaaaagac
     6061 tgcgtgggca tatatggatc ttccttcaat gtctgaatca cttcttcaaa ttgtttcttt
     6121 aaattcgatt gcttgatttt tcttaaatcc actgtcgcag agtttttgat tgcgactgta
     6181 tagttactca tcaaagagct tcccaatcaa tatcatcgat attcgtaaag ccactgtcgt
     6241 ctttttcgcg gtctcgtact ttatctaaag tacctgtttg ctcaagaaaa agggtttctt
     6301 gaatcgctct ccagtcttct agcccaatca atacagcatt gtttccacta cgatcactaa
     6361 taatctcgat ttcagtatga ttttcattta cctctttcaa taactgataa aaatttttac
     6421 gagctgtgct aggattcatc gttgtattag ccattgtctc tcctcctttt tctgatataa
     6481 ttataacgta cttgattaag tacgtcaaaa aatacaataa agcacttaaa ttcagcaaac
     6541 ttttcctctt ttttacatag agaaaaagac ccctttttag ggaggatctt tgtccacgct
     6601 cacttcttac tcctttacga ctagcggtag ccaaacgtaa agtaattggt aacaacgtat
     6661 catctttagc aaaggtcgaa tccaaagcct gtgagtctat gcaacaacac caatacacga
     6721 aggttcctcg ttgcagtgtg acttgattga tatcttgaat aacctgagga tacccacgaa
     6781 agatttgtaa cggttattca aaactgattt tggccgtcaa gtcttctact gcctcaaaaa
     6841 tgattgcctc tttcgtctcc tgccattcat gttcactatc gatttgatac ttgcttagac
     6901 cgtcgggttt gtgaatcacc aattaaaaat ggcactgaat tatggtaaca aaaaatggtc
     6961 acttgacgag gctgagattt ctacttatga agacacatgg cacttaagag tcgaggtaaa
     7021 ggaagaagct gaaaatatag gaggctgtac attatttggc aaaggattcg gaaattatga
     7081 tcaagacatc aaaatcaaaa tgttttacag ctaaatagga agaataataa catttcatac
     7141 gctgttccca tctttaatgg cttatagagg gcacctttag ctgggaataa gacaaaacag
     7201 accctctagc ttcaattaag agctagaggg tctgtttttg attttaaaac ttggtttatt
     7261 tcttcaaatg gtagctatag gtgctgacaa cgatattttc acgccaagac aatcgcaatc
     7321 ttaaccgcaa acttgtttgg ttatggagaa tattctgcca gcgtctgttt ggtacaaatt
     7381 gaggaatcaa cacagtagtt gtatagtttt gtttcgctgc atttttactt acgatatcta
     7441 cataacggat aattggattt tcaatcgagc gataagaaga gtggacgatt gaaaaacgga
     7501 catctgggaa atgcttttta aattctgctt cgatttcttt ttctttttcg acatcttcgt
     7561 ctagcgaaac atgcatcgca acgacataat caccgatcga acgtgcgtaa ttcaacgcac
     7621 cgatatttac ccgtgtcaca ttcccgacta atacaagaac ggtattcccg tcataatcat
     7681 gtagttttgc atcattttct aaacgaagct gttctgctac ttttttataa tgatcatgga
     7741 tcttataaaa aatgaacata acgattggca taatgatgaa gaaaggccaa atgtccccta
     7801 accgatagac gaacaaaatc gcaatgattg catatgagat gaacgcacct gtaatattgg
     7861 caatcgactt gcttaaccat ctttttcctt cttttttcca tttgacgacc atccctgttt
     7921 gagataaagc aaatggaatg aacacgccga tagagtataa cggaatcaag cgttctgttg
     7981 agccatggaa aataaacagc aatacgatcg aacctagcgc tagtgtgata atcccattgg
     8041 aatagcctag tcgatcccca cgatcttgat acatgtgagg catgaattta tcttttgcca
     8101 gattgtatgc aagaactgga aaagcagaaa aaccggtgtt agcagcaact gctaaaatca
     8161 gcgctgtagc aaattgaagg acgtaataca aaataccatg tccaaagaca gctttaccaa
     8221 tttgagacaa aacagtgact tctttttcag gaacgatccc ataccaatag ttgataaatg
     8281 tgatccccac gaagaagaaa cctaagatca atgccatcat cgtcaatgta gcagcggcat
     8341 tttttgctct tggcttctta aagaatggta cagcattact aatcgcctcc accccagtta
     8401 aagaggaaga accagaagaa aatgctcgta aaatcaaagc gatagaaaca cccggcacag
     8461 ccgctcctgg aagtgcagta gcttgcaatg gcacagcacc tgtcacgata ttatataaac
     8521 caacaacgat cagtaacgag atcacaagga taaatgtgta caccggaaat aataggaacg
     8581 atgcagattc cctcagccca cgaagattca gcatcatcag tagcaaaacg attgtcacag
     8641 aaatagcgac ttgatgaccg tagagtgctg gcaccgctga tgtgatcgct tcagcacctg
     8701 ctgagacaga tactgctact gtcaacatgt agtcgatcaa taaagagcca ccggaaatca
     8761 agccagcatt ctttccaaga ttctcactgc tgacgacata tgctcccccg ccatgaggat
     8821 aagcatggat gatttgacga tatgatagcg ttaatgaaat caataaaata ataacaaaag
     8881 cggcaatggg tagcgaatac cagatggctg cagcagacaa agcgactaaa acgacaacga
     8941 tttgttccgt accatatgca atagatgata acgcatcaga cgacaataat gccaaagcag
     9001 caaatcgggt taatttgtgc tcatcatttt cagcagattt taaaggctta cctactaaca
     9061 acctttttaa gtaatccaat ttctaaactc ctttacacac aattttactt caagttttat
     9121 agtcgaaaat ttatagtaaa caagatgtta tagtacgctc actgacctta aaagtcaata
     9181 aactttaaga aaataattgg aaaaaaacag aaaaatatca agcgcttttt caaacgcttt
     9241 aatacagcaa aaatctaaca tttttttatg ggctttttct ttgaattcta taataaaagg
     9301 atagaacttg acttagaaaa tcaagttcta tcctttttga tatcttaact tttgaaagag
     9361 agatgtaagg ttgctttcta cccatcattc ttactactcg aagcatagcg cagttgtcac
     9421 aacctcttga taaacggtgt ttaaaagctt gcatctgcgg taataagccc aaccaaatta
     9481 aaaacataag aagctgtttt tatttggtcg ttcttattac ttgatgcaga acagcctttt
     9541 cacaacctct tggtacacgg tgttcaatca gctgaccctc ggcattagct caaaaaacgg
     9601 caaaatatga ggaactattt ttccattttt ttctctaata ctcaggtcaa aacgctgatt
     9661 tcacaacctc tttttttaat gaaaaatatg gctctcatgc gccgcttctt tgaatttcgt
     9721 cattttttct tcgatgaacg gatagattgc cgttcctacg attccaaaga caacaaacag
     9781 accgatcatg atccagattg ctttcgtagc atttggccag taaatgcctc ctgcagattc
     9841 ccgtaacaag tttaccgcat gagtaaatgg caagaacgga ttgatcattt ggaagaattt
     9901 ccctgagact tggatcggat agttcccgcc tccgccagag atcgacagta ctaagatgat
     9961 aatagcgatc cctttaccga cattatcgaa taatgcagcc aagacataga cgatcatcat
    10021 gaacgctaag gcgatcaaga cagcaaatga cacgctgtag accggatttt ggacatctac
    10081 tcctagtaag aaaatattcc ctaatgtaac gatcaagctt tgcgcaatcg ccattgtcaa
    10141 aaatgtcagc atacgagcaa cgaattgttc tcgtttcgta aaattgtttc ggtctttctt
    10201 tcctaaatag tattctgtcg tagcaacact tgaaagaaga acagcgccga cccacagaca
    10261 tagtgcagta taaaacgggg tgctagctga accgttattt gcgattggat aaagcgcatt
    10321 ggtttgtagc tcaacaggtt gtgtaaagaa gtcactttct tttgttgcat ctagtttcaa
    10381 taatttgatg acttcgccta gatcgacttc ttgttcgcct tttcggatcg cgtctgctgc
    10441 tttttggata ccagctttga tatttggcca gtcattgttg atcaagtccg cagcagcgtt
    10501 cacagctgtt tcgacttctg gcattttttc atttactgtt gtcaatgtac cggtgatttc
    10561 attttttagt gctggccaat cattttggat aaaatcagcg gccaatccta atttttgctg
    10621 aaggatcggt aattcattgt tgtataggtc tgccccttca ttgatgccat tgacgatggt
    10681 atccatattt ccattcagca ttgtattggc atcatggatt tcttgtccaa ttgcaggcat
    10741 ttctttttga tatttttcta aaattgccac tgcattcgtt acagttgctt tcgttgaact
    10801 gagaagcgaa tcaaagtcga tctggtccgc tttgcttagc gccccttgcg cagtcccaat
    10861 cgtatcgatg acttggtcta atagagaagt gacagtggaa ctgatttgac ctgtattgat
    10921 accagcaatg atgccattga tattattaac aacggcatca atttgatcta gcaattcatc
    10981 aatagccttc gtgtcttcat tttgaacagc tgtgttcaaa gcagataggc ggttttgcag
    11041 attgcttagc aagggtttta attgatttag ctgatcaata attggctgca atttcgtatt
    11101 tccagcactt gtttgcaatt tagtcaggaa ctcgaccagt ttatcgattg cattttgttg
    11161 ttgagatatt ccgtcaataa agccgttcaa tacattgctg atgtttttct tttcatcatc
    11221 agtcagctta ccatcttcaa tagctgttct tacagtatct attaacgaac ctgtcgttgt
    11281 tgccacttgt ccaattgatt tcaatgtaac atctacactg tttgtgatgc ttggtatcgc
    11341 atctttcagc tgagaggcac ctgattttgt ttggtttgct agatcacttg cctgattttc
    11401 taaatttttc acatctggca agatatcctg tacttgttgg atgatcgtca agccttgttt
    11461 tgcttcatcg attccttggt tcattgtctc ttggatcgaa gcaaaatcgc cgtcgatctc
    11521 tgctagttgt ttaccggcat tttggatctc tgggattttt tcttgcaaat caaggatgat
    11581 ttttgcttga tctttcaact caggcatttt gtcattcaat gcaacgactt tttcacccat
    11641 ctcatctact ttagggatat aatccacgaa ttcatttgct ttattcagtt tttccttgat
    11701 ttcaggtagt tgactttgta attcgaggac ttgttttgtg tagccgtcga tcgtgtccaa
    11761 attttcatct gttgataaaa tcatatcttt gactttattg atacttacta ggttcgtatc
    11821 gatgtcgtaa cctatctcat tgaatacttt caataaagtc gaactggctg ttttgataaa
    11881 ttcatttgtg atttgcgatt ggatcgaact tgctccttta tcggtgattt tcggtgcaat
    11941 cgcattgatt ttttcattta ccgtatattc gatttttggt ttcttgatat caccactagt
    12001 aaaactcagc agatcttctg agaaatcctt tggcaagtaa atacctgcgt aatactttcc
    12061 ggaacggact ccgtcttcta attgttcttt tgaatcaacg aattgccatc ctaattgttt
    12121 atttttatga agcgattcga tgacttgctc cccaatggcc acttctttac cttgaaactc
    12181 tgctggcttg tctgcactgt aaacagcaat tggcagctct ccagtatttc catatggatc
    12241 ccataatgct ttgatgttaa accacgcata tagcgaaggg atgatcatga tcgcgatgat
    12301 cagaagtgtt gcaatcgggt ttttgaaaat ccgtttccag tcaagcaaat ataatcgaag
    12361 cgtatttttg attgactgca tagttctccc ccttcttctt ttttcagctg aattacttta
    12421 ccatgtattt catacttttt tcaatcggct tttttcaaaa aagtcgaatt tttggacata
    12481 tcacggcaat tgtctcattg taatctctta catattgaga aataattaag gattacagac
    12541 tttactttat catgaaataa aagggaaaga taattttatg ccaaagtttt tatc
//
LOCUS       DACEUZ010000083        12204 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.083, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000083 DACEUZ010000000
VERSION     DACEUZ010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12204
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.083"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(147..488)
                     /locus_tag="IXG44_001875"
     CDS             complement(147..488)
                     /locus_tag="IXG44_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAQ1658592.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(556..2727)
                     /locus_tag="IXG44_001876"
     CDS             complement(556..2727)
                     /locus_tag="IXG44_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAQ1658593.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            2938..3795
                     /locus_tag="IXG44_001877"
     CDS             2938..3795
                     /locus_tag="IXG44_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAQ1658594.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            complement(3866..4723)
                     /locus_tag="IXG44_001878"
     CDS             complement(3866..4723)
                     /locus_tag="IXG44_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ1658595.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(4708..7410)
                     /locus_tag="IXG44_001879"
     CDS             complement(4708..7410)
                     /locus_tag="IXG44_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAQ1658596.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(7423..8712)
                     /locus_tag="IXG44_001880"
     CDS             complement(7423..8712)
                     /locus_tag="IXG44_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HAQ1658597.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            8862..9908
                     /locus_tag="IXG44_001881"
     CDS             8862..9908
                     /locus_tag="IXG44_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1658598.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            9910..12063
                     /locus_tag="IXG44_001882"
     CDS             9910..12063
                     /locus_tag="IXG44_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAQ1658599.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
BASE COUNT         3362 a         2575 c         2230 g         4037 t
ORIGIN      
        1 tccttgtaac tcagagttaa acggtaggct cacgacctct tggtaaatgg tgttcaagag
       61 acagcctatc ggcaataaga agcaattttg tcatcgctca aggccgaacg tctctttcac
      121 accctctgtt gtttcataaa atagttttat tcgctgtaaa ggtcgcgaac tggtgtcatg
      181 atgatacggt tcaagtcatt gatgattgta ctgaagcctt gctctttttg catcaagtca
      241 ttgattactt cttctgattg gattttcaca gccatttctt gtgctttcat tgcatcttcg
      301 tcgctgaact cttgtccact catttgtttt tcttgtaatt cgatttgcat cgcttggaag
      361 tctttgaaca gtttgtgtgc ttcttcgttt gcttgtactt tctcgtacgc ttctttcaac
      421 gctttgaatt ctggtaattc acggatctca cgttcgattt gattcgcgct atcgtagatg
      481 tttgacatgt tatgccctct tttcttttag tagttactct ctattgtaac atggggttat
      541 tcgttttcaa aggggctact ctccttgata tttccgccag aggtctttag ccgcttcacc
      601 aattccctga acgccttctt tcaccatatc gccgaagttt ttcagccctt cttttgcttg
      661 ttcaccatat tgattcaagt tatcttgcca atttccagac tctccattag aatttcctgt
      721 gtttccaact tgatctgcag ctaccacttg tcctcctgtg gcataggcat ctgctacact
      781 aaatcctgtt tttgaagtat acggaaggat gccttctgcc gctgatttga agactttccc
      841 gacaacattt ccacttgtcc cttccaaata gtgcagcttg ctggattctt cgtaacccat
      901 ccatgtcgaa atcactacgt ctggcgtgta accgatcatc cattggtcat ttgcttttgt
      961 ggcatcgaaa ttagtttctg tggttcctgt tttccctgct atcgtataac ctgcaggatt
     1021 cgcagcagcc gctgttccat ttgaaaatgt accaagaagc atgctggtca tttcatcggc
     1081 tgtttccttt gatatgacct gttttggttt tttgtttgtg ttatcgacga tcaccgctcc
     1141 agtcgaatca acgattttcg tgataagatg cggcgtatac atctgaccat cattggcaaa
     1201 tgcactgtaa gcacccgcca tgatcaatgg agatactcct ttttccagtc caccaagggc
     1261 taagccataa tacttatcag attttgctaa aggcaggccg aattcttctg ctttatcata
     1321 tcctttttgt aaaccgatct catgcaaaag ccatacagta ggcaaattca aactttgtgc
     1381 aacagcttga tacatcggta cttctccttg gtatgtcccg tcaaaattat gcgcatcgta
     1441 ataactttgc ggttcgtctt tcaagatact ggaaggcttg tatccagctt ccagtgctgg
     1501 tgtatatacg cttaatggtt tgatcgttga tcctggtgaa cgacgcatct gtgtagcaaa
     1561 gctgaatcct ctaaagacat gatctcctct tcgtcctact agtgcttgta caccacctgt
     1621 attcggatct aaagctactg aagcagattg gaccatggca ccatctgaag catttgctgg
     1681 gaataatgca ctgttttgat aagtagcttc taattgcttt tgataatcct gatctaaagc
     1741 tgtatagatt ttatagcctt tattcatgac atcttcttca tcaagaccat actcgttaac
     1801 tgcttcatcg atcactgcat caaaataata aggataacga taaccagaat ctgaattatc
     1861 gtatgtgtca ttaagatacg cgctgatatc tgtctggctt tctgcttgtt cttgttcttt
     1921 tgtgattttt ccgttttctt ccattacact tagtactgtg tttttccgat cattcgcatt
     1981 ttttgggtga tcgatcggat tataaatacc aggacctttc agcattcctg ccaaagtagc
     2041 agcctcagac agcgtaacat tcgttgcatc tactccaaaa tactttctgg aagcgtcttg
     2101 gacaccccat accccgttgc caaaataggc attgttaaga tacatggtca agatttcttc
     2161 tttgctgtac ttcttttcga tctcgattgc caaaaataat tccttcgctt ttcgatcaaa
     2221 cgtttggtct aatgtcagat acgcattttt ggcaagctgc tgagtgatcg tacttcctcc
     2281 gccccctgat gttccgccag acaagaccat tcggactgct gcacgggcaa tccctttgat
     2341 atcaaacccg tgatgctcgt aaaaattcct atcttctgtc gagattactg catcttggat
     2401 atagggagaa atcgcgttta attctacata tgttcctttt tgggcgtaca gtgtgccagc
     2461 ttcttcgttt ttcttatcat aaatgactgt cgattggctt aatcctgatt ttaacgtttc
     2521 gacatttgct tgttttgcta aaataaagag gtagatactt gtcactaaga cacagatcaa
     2581 accaatcagc aaaatcaatt tgttcacatg atattttttc cagatgcgct ttcgccatcg
     2641 atggaattgt ccgagatatg gtctgatcca acgccaaaaa ctgatcaaag cagcttttat
     2701 cttttctaaa atgctttgaa aatccatttg cttcttctcc tgtctatttg attcaatcgt
     2761 cttattttac catagtgaaa gagtgttttc ttcctgctta agttgtagtt caatatgttt
     2821 ttttacgtta gtgcaagtag agtaacataa gaatgaacag aaaaatgtaa gttttttctt
     2881 aaatatgttt taaaaatggt acaattagtc tgcgattgca ctgaaaggag tacacttgtg
     2941 aaaatcacga tcactttacc tatgaaccat cctgaaacaa caattcgcga cttgcttgaa
     3001 aaggaatggt tagttcctag aaaggttcga cactttttgc gtatacgtaa aaacgtttgg
     3061 atcaatcaac aacctgcttt gttccatcat gaagtaaagg gcaatgacca gatcacattg
     3121 ttattcgaag aaacagatta taattatcaa gaagttcaat taggagatgc gaagcacatc
     3181 cgtgttttat atgaagatga gcatttgatt atagtggata agcctgcagg aatgaagact
     3241 catccaaatg aaccgacaga aaacagtacc ttactcaatc atttagctgc ttatctcgac
     3301 actaaacagc aacgacctta cgttgtccac cgcttggaca aagaaacaag cggtgccgtt
     3361 ttatttgcaa aaaatccatt tgtcttgccg atacttggaa gaatgttgga acaaaaaatg
     3421 atctatcgga gatatcaagc catcgtatgg ggagaaattg ccaaagatat gaccatcacc
     3481 aataaaattg gacgagatcg ccatgatcgg agaaaaaggg tcgtagatga taaaaatggt
     3541 cagagcgcct tgacacatgt cactgtcaac aaagtacttt ctgaacaaac acaagtctat
     3601 tgtgttttgg aaaccggacg tacacaccag atcagagttc atttagctca taagggacat
     3661 ccgatcgttg gagaccctct ctaccaaaat agaacagctg aacgactgat gctccatgca
     3721 ttagaactgc acatgatcca tccatttact caagaaaaaa tagtcggtaa agctttacct
     3781 ggattatggg aatagaaata aaaaagaaca gagtcttatc agcagataat gataagactc
     3841 tgttcttttt gtgtaatttg tctgcttact tttgatgttc gtgttctgct aacagcaaac
     3901 taccgataat cccactttca ttcggcagtc cccaacgaac gatatattca cttacttgag
     3961 gtgtttccat ataaccaccc atcaattcaa caaattgctg acggattttt tgaaggagat
     4021 gatcctgatt cattacccca ccgcctaaaa taatcttttc aggtgctaac gtcaacgtat
     4081 agttgatcag tgcttgagca atataataag cctgaatagc ccaaacagga tgatcttgcg
     4141 gcagatcttg ccctttgatc cctgtgcgtg cctcaataga cggtcctgct gctaatcctt
     4201 ctaaacaatc tttatgataa ggacaagtac cttcataatg gtcatctgga tggcgtttca
     4261 cccaaatatg tcccatttca ggatgtgcga tccctgaaaa aatttcttca ttgatcacga
     4321 cacctgcacc gattcctgtt cccacggtaa ggtaaataca actatttttt ccttgtgctg
     4381 ctccttgctt caattcccca tatgcagcag cattgacatc cgttgtccaa gcagcaggaa
     4441 tattgtagcg ttccttgatt gtgccaagaa aatcgaattg tgcccaccct tttttgggag
     4501 ttgccaaaac gtatccgtaa ttatcttttg ttttgtcgat accaatcggg ccgaatgagc
     4561 caatcccgat tcccttcaat tcataacgat caaaaaattc aaatacctga tccaatgttt
     4621 cttctggtgt tgtggtaggg atacttaatt tttctaaaat ttctccacca tctgaaaccg
     4681 cacacacaaa ttttgttccg cctgcttcta ttccgccata cattcgtttt cctcctcgaa
     4741 tccgattgtc cgaatctcat aaggttggat tattgggaca accgcttgct tcgttggttc
     4801 ttcaagcaga ttcaatatga cagtttttcc attgtcttta gttatttcca atttttctaa
     4861 atgactagac atattgaaac ctcttactac tactttatca ctaaacttgc tgcgtttcaa
     4921 agctgtaatt gcaaacgttt cactttttat cgttaaatat tgttgttttg aaatcaatgt
     4981 tccttcatga tgtttgattt gttgagtgga aaaaggaatc tgtgctgcgt atgcatgcaa
     5041 ataagtagag aatttttctt ctggtccatg tagttcaatg ctgtaattga accgatgctc
     5101 acctagacac tgtgcttctg gtgttggaaa atatccccaa tcgcctagtt ctcccacact
     5161 gcgaagtagc gtcactgcga tttgtccgtc ttctgttact tcatattcat tcaaaccaaa
     5221 gtttccgact gttacaccgt actcttcatc atgaatattg acaaatgctt gctgatgttg
     5281 cggattcgtg gggttttccc aacttttaga cacatggttt gggcgcttca ccacttcaaa
     5341 aatactatct gcttcgtgat gttccacatg taactttgta ggaaacaaga cacgtaagcg
     5401 atgatctttc atctgattgt cgagagtcgt ttcaaagtcg atttttttac tatcttttct
     5461 gattgtcatg attgttttta tttgaaggat tcgtttttct ttggatcgtt ctgcttttcg
     5521 ataacgaaat tccacaacca tctgctgttc tttttctaaa cgctcgtctg cagaaacagg
     5581 gatttccatt tcctgtagaa tacttatttt tgctatttcc ggtgtatcta ctaatacttt
     5641 tacttcagag ttttctttgt tactggatag gattggctga tcacagaaag gttgtttaaa
     5701 gatatactca tttcctatat cgccgatatc ttcaaaaata tgcagattat tcaactttcg
     5761 tttgtttttg cgatcttcca ttgtaatggt accatttttt tcaatcgtca aatgtagagg
     5821 gccgttttcg atgattcgtc cactctgatg aatcattgtt tccttttctt ctgctttcgc
     5881 ttccccttct acaagcgcga atgtttccca agaaaatgct ggcatgtttt ctaatggtaa
     5941 agtcactttg atcattcttg ccatatatgg cacacggaaa cggtctttcg gcaaatcata
     6001 accaaattgg acagtttctt cagataaaat agctggtata gtggccccac tttttgtttc
     6061 tacatgatac ttacgtttag gcaaattttc taactcttga tatagttgtt caggtattcc
     6121 ttcagcgaat ctttttcttt ctaattcgat ggttatttct gcttcaccag ttttatccat
     6181 tcctgcagta tttacaatga caaatggaaa gctgtctttt gggaatccgg tcgtatcgat
     6241 tgctgcggtc agctgttcta aggaatcttg tgcaagatac tttccaactt catctgcttt
     6301 ttcaaaacga ggtatcattt cccgatggac agaatcgact gaacaaccgc aaatactgtc
     6361 gtgaggatga ttttgcatta atgtcttcca agcataatct aattgatcat ggggatagtt
     6421 tccactaact tcataagcca tcgtcgcaag tggttctgta atattttcta gctgtcgctg
     6481 gaccttggta ttccattgtt ttaagtaaac ccgagcagat gctgtattgg ctaaagtata
     6541 ccagccatcc gtttcttgac ttgtcaattc tccttctact gagcctaagt cttctggcac
     6601 atctttttga actgcttcca gatagtcagt aaaattagaa tgaataaact cgtaatctgg
     6661 aaacagttca tttgcgagat ggattgcttt tgatatatct ttttgtaccg gttgatgatc
     6721 aacaccattc atcatcaata agtggttcgt agaggcatat tgttctgcat ccgctaattt
     6781 ttgcttccaa aaggcaagag ctgcttcttt ttctactgga atctcattcc cgttgctata
     6841 ccaattggca aatagcagac caaagattgc tgtttgatca ggtcctttcc accacatctc
     6901 tgagtattgg gaagaatagt tttctgcttc caaaacttga ttatcaaagc caattggttt
     6961 tacaccacgg ccaaacgctg ctgctgaaat accagcttgt ttcattaatt gaggcgtctg
     7021 tcccatattt ccaaatgtat cgggaaaata gcctaacatg acaggggttc cccatttacg
     7081 gctttcttcc atgccaatca gcatatttct cacgttcgat tccgagctga taagaaaatc
     7141 atcttgcaag atataaaatg gtccaattct caattttcct tcattgattg cttgttggat
     7201 tgcctgtctt ttttctggcc ttacttgaag atagtcatct aaaataatcg tttgcccatc
     7261 caaatgaaaa ctgttgaagg acggatcaac ttcaaatagc tctagtagat cgtctatcaa
     7321 ttcaatcagt cgcatgtgat gctgttcgta agccatgtac cattcacgat cccagtggct
     7381 atgagaaatg atatatactt tctttttcat ttacgatacc tcctactttt ctactctaat
     7441 atcaaagtaa tccatgacta attcgcaaaa catcatattt gcccaagaaa accattctct
     7501 cgtataattt tcaggattgt tcacatcgaa tccttcgtgc ataaggtgtg ttcctgcgtc
     7561 agttgctaca agtaaattca atatccgctc tttttcagat ttgtctgacg ttgtcattcc
     7621 ttccatagct aatgcaatcg gccaaacgta tttttctggc gtatgggagg atccaattcc
     7681 ttttgcgtac tttccttcat agaaatatgg attttcttta ctcaacaatg ttcttcttgt
     7741 ttgcagataa tcttcctctt gttcagaaca atatcccaag taaggtgcgg cgatcagatt
     7801 gggcacatta ctgtcatcca tgactgtggc gtttcccatt ccatctactt catatgcata
     7861 aatcgtttgc cctttttgat taaccgttcg tccgtgattc tgaatcccct cttggatctc
     7921 tttcttcaat ttctgtgttt gctgtacaat ttctttatca tcgagtatcg tttgaaaaat
     7981 ttcttccaca taacctaaca cgaccaccgc aaacatattg gatggtacca gatatccata
     8041 acggcaagta tcatcgctag gacggaaacc agaccatgtc attcctgtaa aagctgtttt
     8101 tgcacctcgc ccgtcattga tcagcgtatc ttcttttctt gtcgtgtctc gtacaaatgt
     8161 ataaggagag tgttcatgat cttgctctgt tttgaacacg tccaagattt ttttgatacc
     8221 ttcaataaaa gattgattga attgatcagt ttttcctgta tttttgtaaa gaagataggc
     8281 caactgtatt gggtagcata aagaatcaat ttcatatttt ctttcccaga tccaatcgtt
     8341 catttttgta tgatctgttt ggtggccagc accgttagct tcttcgttga aggcattcgc
     8401 atatggatcg atattgatat aaaaaaattg ctttttgact aacccgctga tcatgtcagc
     8461 taattcctca tcttcttttg caatcactaa ataaggacga acttgtgcag tagagtctct
     8521 taaccacatc gctggaatgt ctcccgtcaa taaaaaggtg gttccgtcat tttgcttttt
     8581 gactgttgtc aacagagtat tggcaaatgc tgctgtaaaa ttctttgccc agtccgcatg
     8641 gatatcccca catttttgtg tgatatcttc cataaatttc tgcaccgatt ttggtatatc
     8701 tttataagcc actaataaaa ccttctttct atttgatata tctttaaaga ttatacttca
     8761 tacagccaat tatgtctacg ctttcaaaaa atatcttttt atttactttt tcatccatta
     8821 tggtattatt tgaatatgat atatcattaa gaggtgttta tatgaatcaa ccattgtacc
     8881 agacaatatt taatgatttg caaacggcta ttttaactgg tgaattacct gtagacagcc
     8941 aactaccaac tgaaaaagaa ctttcacgta cctataaagt aagcagaatc acttccaaac
     9001 gtgcattgac tgaattagag caattaggac ttatttatcg cgttcgcggg aaaggcagtt
     9061 tcgttaaatc cgcttctccg gaacagattc ttgcttctcc tacaaaaaca cgacggattc
     9121 tgtttcttct tccatatgtc gctgatttgt ctgttggtga ttttagcaag ggattgaacc
     9181 ctgtgatgca agataaacag attgatgtca tgatgacgac gcttgacttt ctacaaaata
     9241 aaacagcaaa agagctaatc aatgaatttg acggattgat ttattatgct cttgatacag
     9301 aacaacatct tgacttactt tttgagcttt cattgaaaga attccctgtc gtcatattag
     9361 ataaaaaaat ctatgagtta ccttttccta ccgttctttc cgataatttc caaggcggtg
     9421 cgcttgctgc ctcttatttg atcgaacagg gacattcaaa aatcgcttat ttgtttggta
     9481 cgcagactca tccacaatcc gttcgacagc gttaccttgg ttaccttgaa gcattggaca
     9541 aagctggttt gactttccac acttcactgg atgacaaaag cgcgacatta gatgaattgc
     9601 tttcttatat cactaagtat gaagtaactg cttttgtttg tgaaaacgat gttgtcgcta
     9661 tccagaccat gaatctcttg agacagcatg gttatcatat ccctgaagat ttttcaatta
     9721 tcggatttga tgatatccaa gcagcagctc ttgttgaccc gccactgaca accatcgcac
     9781 aagattttga acagttaggt aagcttgcgg gagatgcttt ggtcacttgg ttagagacaa
     9841 aagatcgacc atccgatagt aaatttccag tttcattcat caaaagacaa tcaacaaagg
     9901 agaaataaaa tgaatatcgg tcaaatagat actagacatg caacagcgaa tcagccaaat
     9961 ttctctaatg ggaactgcca gccttatact ggcgttcctt ttgggatgaa ttattttgca
    10021 ccgcaaacta cggatcaaaa cggcagctgg tggtttcatc ctgatgaccg cgtttttcaa
    10081 ggctaccgtc ttacccatca accaagtcct tggatgggtg atttcagtca tatgctgttt
    10141 acgccgatca atggaaaact acaggagaac acccttttcc atgctcaaag ttcttaccgc
    10201 ccagaagaaa gtatattttg cccgactcat ctgtcgatct gtcaattacg ttatggcatc
    10261 tgttctacat tgatacctag catgtatggc ggaatcttat ctattgatta cagcaaaaat
    10321 gacagcggtt tacttttatc ctttccggga cgccatcagc tctttgttat agatccttac
    10381 acagtggaag gaaaaatcac acattttgct ggttcagaag atcctgattt ttctttttat
    10441 tttatattga gatttgaata tcctttaaca attgatgagt tagctgtttc caacaaaaat
    10501 agcgactcga tcccgttgta ttttgaacag acaaaaaaac aaactatccg atttgggaca
    10561 tcttttatca gctcggaaca agcagcttat aacctggaac tagaacaaaa ccaaagcaaa
    10621 gagacttacc ttgctcacag tcaagaacaa tggcagtcct attttgatcg tatacagctc
    10681 acacaccatg atcaacaaca attgcggacg ttttatcata atttatatcg ggtgttttta
    10741 tttccacaaa cattttatga aattgatcct agtgggcaaa aaatccacta tgatacgact
    10801 agtcgatcta taaaacctgg tgttctctat acaaataatg ggttttggga tacgtataaa
    10861 acggtttatc ctctttattc tttgattgct gtagaaaaat acgaagaaat gctggaaggt
    10921 tttttgaaca gctatagaga aagtggctat ttgccaaaat ggctgtctcc tgatgaacgt
    10981 ggacttatgc ctgggacatt gatcgatgcg gtcattgcag atgctgctgt aaaagatatc
    11041 cgacgagact tgatgcctga atttctaacg gcaatggaaa aaggtgctac ggttcaaagc
    11101 aaacatccaa attatggacg ccaaggaaca gaagattact tgacatatgg ctatgttcct
    11161 aatcactacc acgaatcagt caatcacaca ttagactatg cttatagcga tttttgtatt
    11221 agccaagttg ccaaggtact agaagaagag gaaatgacga agaaatatca gcagcaatca
    11281 ttgaactatc aaaatatctt tgactatgag tcaggattta tgcgttcaaa agataaagat
    11341 ggcaagttca gagaaccatt ttcagctaat cgttggggac aagattatgc agaaggaagt
    11401 gcttggcaaa gcagctttgc tgtttatcac gatttttcag gactgatcgg aaaatatggt
    11461 ggcagtgaga gatataaaga aaaactcatt gagctgtgca atcagccccc aacgtttgac
    11521 ccaggaggat atggttttga gatccacgaa atgagtgaga tggccgcgat agaatttgga
    11581 caattagcta tttcaaacca gccaagtttt cactatcctt acttgttcag ttatatagga
    11641 caacctgaaa tggcacagcc actgcttaaa cagttgatga ctcagacctt cgacgattct
    11701 ccaatgggtt accctggaga tgaggacaat ggaagtatgg ccgcttggta tatcttcaat
    11761 agtctgggat tttatcctgt cacgcctgga accggtgaat atgttatcgg tatgccatta
    11821 attgacaagg ctgttttgct tttgtcaaat ggaaaggaat tgacgattga agcaacgcct
    11881 aatcggccgc agcaacaatt tatccataaa atcaaacgga tggacaaaga atatacgaaa
    11941 ttatatttta cccatcaaga tttgctgaaa ggcggcacga tctcttacca attagggatc
    12001 atccctcacc ctaagaaata tcaagcaaaa gatttgcctt tttccttatc aaactataat
    12061 taacaagagg ttgtgaaaaa gctgtcctgc atcaagtaat aagaacagcc aagcaaaaat
    12121 agatcctcat atttttcgtt tggttggtct tattaccgca gatgcaagct tttgaacacc
    12181 gtttattgag aggttgtgaa atca
//
LOCUS       DACEUZ010000084        12171 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.084, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000084 DACEUZ010000000
VERSION     DACEUZ010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12171
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.084"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            592..1920
                     /locus_tag="IXG44_001883"
     CDS             592..1920
                     /locus_tag="IXG44_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAQ1658600.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2122..2955
                     /locus_tag="IXG44_001884"
     CDS             2122..2955
                     /locus_tag="IXG44_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HAQ1658601.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            2971..3708
                     /locus_tag="IXG44_001885"
     CDS             2971..3708
                     /locus_tag="IXG44_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HAQ1658602.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3863..4831)
                     /locus_tag="IXG44_001886"
     CDS             complement(3863..4831)
                     /locus_tag="IXG44_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HAQ1658603.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            5063..5896
                     /locus_tag="IXG44_001887"
     CDS             5063..5896
                     /locus_tag="IXG44_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HAQ1658604.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            5930..6769
                     /locus_tag="IXG44_001888"
     CDS             5930..6769
                     /locus_tag="IXG44_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ1658605.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6795..7355
                     /locus_tag="IXG44_001889"
     CDS             6795..7355
                     /locus_tag="IXG44_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAQ1658606.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7554..9275
                     /locus_tag="IXG44_001890"
     CDS             7554..9275
                     /locus_tag="IXG44_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HAQ1658607.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            9440..10582
                     /locus_tag="IXG44_001891"
     CDS             9440..10582
                     /locus_tag="IXG44_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAQ1658608.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            10598..11809
                     /gene="thiI"
                     /locus_tag="IXG44_001892"
     CDS             10598..11809
                     /gene="thiI"
                     /locus_tag="IXG44_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HAQ1658609.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         4149 a         2042 c         2526 g         3454 t
ORIGIN      
        1 aaagcttatg caaagaaaaa gaaatccaat cacttgggtt tctttttctt ttattattat
       61 gatgaatcaa gctttctgtt tttgtcacgt atgttgtaat ctttacgaaa gagtagagtt
      121 ggctactcat aatcaaccat acttcccatc atcgctaaat acgaaataaa tcagcattcc
      181 tacaccaatc aaagcacaag ctgcaccaag aaaagggatg acattcccaa agttatctgt
      241 attcataagt atgccaccac ccacaaacaa aaagccgcca gcaatcaggt ttttcatttt
      301 attcatctcc tttcttacgt tatagtattt ccttctttca atgagtattt tgaaagctat
      361 cttattgtat gatgttcttc taggcctctt ctttattata caggccattt tcgttatctc
      421 atagaaatat tcctttttat ctgaaaacta ttgtataccg tatgacattt ttcttcatga
      481 agttgtaagt atattctcat tcaaatgaaa atttcatcat agaagagatt gtatttctaa
      541 attcttaaga gtaaaatacg aagtataact tggatttgga aggatgattg gatgaaagct
      601 attacgcctg atatgacaac aaaatttaac aaacagtttc aagcaaatat caaacagcag
      661 gcattgcagc gggcagttgt gaagaacgga attactgctt ctgctgaaaa tgtttcagca
      721 catgtggaga atacaccggt attttctata gatttggcta cggggaaagt ggctaatcaa
      781 atgcaatcgg ggcgttgctg gatgtttgct gcactgaaca cattccgtca taagatgctg
      841 aatcaattcc atttaaaaga gtttgaatta tcacaaaatt acactttttt ctgggataaa
      901 tatgaaaagg caaattattt ctatgaaaac atcattgcaa caggaaaaga ggcaatcgat
      961 agccggaaag tagctttctt actcgctact cctcaacaag atggcggcca atgggatatg
     1021 atcgtttcat tattccaaaa atatggcgtt gtgcctaaat cggtgatgcc ggaaagtgcg
     1081 aatagttcaa attctcgaga tttgaataac tacttgaaca aattactgcg taaagatgca
     1141 gtagtgttac ggaaaatggt tgctcaagga gaaacattag acgctattga agaaaagaaa
     1201 gaagaaatgc tagaaagcat ttataacttc ttagctatct cattaggaac acctccaaaa
     1261 gaattcgatt ttgagtaccg cgatgaagaa aagaactatc atcttgaccg aggactgaca
     1321 ccacaaattt tttatgataa atacatcggt gtaaaattag atgattatgt tagcgtgatc
     1381 aatgcaccaa caaaagacaa accattcaat cgttcttata cagtagagat gctaggaaat
     1441 gtcgttggcg ggaaagaagt caaatatctg aatgttgata tggaaacttt caaaaaatta
     1501 gcgattgctc agcttgaaga aggagaatct gtttggtttg gttgtgatgt aggtcaatct
     1561 tctactagaa ctagtggcat catggcctta gatgcttatt caatggatga tctatttgat
     1621 acggatttta caatgactaa agctgaacgt ttagattttg gagaaagtct gatgacccat
     1681 gcgatggtat tgaccggtgt tgacttagta gacgggcaat caacaaaatg gaaagtcgaa
     1741 aatagctggg gcgaaaaagt tggaaagaat ggcttctttg ttatgagtga tgcctggatg
     1801 gacgaatata cgtaccaaat cgttgtaaga aaagatttat tgacgaaaga gcaattaaat
     1861 gcgttcaatg aagaaccaat tgttctatct ccgtgggatc caatgggtgc tttagcatag
     1921 acgagtattt caatgaagag tatgtagtaa agataaaaag agctggctta agccagctct
     1981 ttttatcttt aaagtccatt ccctgttttt tatggtccct gtgctataat tctaaataaa
     2041 ggataaggat tggaaataat ccttttatca ttaaaaatat gatgacgaag tagaataaaa
     2101 ttgggagtag gggggaaagt catggatatc caaactgttt tatctgatct gaaattaaat
     2161 ggtaaagtga ttccagttgt tggtggcgat gttaatcaga cctatcgtat aaaaaccgaa
     2221 catcgtgctt attttttaaa aatccatccg aatgtaaaaa aaggattttt tgaagcagaa
     2281 gtagacggat taaaagaact gtcagcattc gtacgtgtac cggatacgta tatgctaggt
     2341 gaaacaagtg aaggcgctta tttgttaatg gaatggatcg agccgggaaa aggggatcaa
     2401 cgagatcttg ctgcagcttt agctaatctc catcagcaaa cagcgccaca atttggtttt
     2461 cgtaaagata actatttggg tacacttgtt cagaaaaatt cttttgaaga agattggtgg
     2521 acttttttct ttaaagatcg gcttgaatcc cagatttctt tggcagaaga aacgaatcga
     2581 tggaatgtac agcgtcaaga aaagtatctg cgatttaaag aacgtgtatt gaaatcggtt
     2641 gaaccaaaaa agatcactcc acgcctcttg cacggagacc tgtggagtgg taatgtgttc
     2701 tttgaccagc aagggcatcc agtttttgtc gacccagctg tttcttatgg gaatcgggaa
     2761 caagatattg caatgagcca gttattcggt ggtttccgac cggaattttt agatgcttat
     2821 caaactatat ttccattaga aaagggatgg aaagaccgac tgccgattta ccagttatat
     2881 tatcttttgg cacatttgaa tatgtttgga gaaagttacg gttcccaagt agatcaatta
     2941 ctagaaaatt tttaagagga gggcgtgttt ttggcaaaaa aatataccaa aaagcatgaa
     3001 gtttcatatt atgagtgtga catcaaccaa acgatgacat ttccttcgat gttaggagta
     3061 gttatcaaaa cgtctgaaga tcaaagcgac cagttgggaa gaggcagtga attcgtcaat
     3121 agctttggac ttacttgggt catcacgaat tattccatgg agattaatcg tctccctaga
     3181 gtcggtgaag taatctcagt aacgactcaa gccatggagt acaataagtt tttctgttat
     3241 cggaacttct ggatccatga tgaagatggg aaagaattag tcaaaataga atctgttttc
     3301 gtattgatgg attttgtgaa tcgaaaaatg agcagtgtta atgaagagat cattgctcct
     3361 tttgagagtg agaagatcaa gaaaatcaag cgtcaggaaa aaattgaaaa gattgattcg
     3421 ggcttcatgt tgccatatcg tgttcgtttt tatgatatcg atagtaatca gcatgttaat
     3481 aatgcgatgt attttaattg gatcattgac gtattaggat atgacttttt gactacgcat
     3541 gttccagaaa aagtaattat tcgatttgat aaagaagtag agtacgggaa tgagatcgaa
     3601 agccattttg aacaaatccc tatcgaaaat ggtgtcaaaa caaaacatga aatccgaatg
     3661 caagaacagc tgtattgtga agcaaatatt atatggaaaa ataaataaaa aagaggttgt
     3721 gacagaaggg ggcaacttca agaaataaga agaaatttac gaaaattgtt tctcaaattt
     3781 ttgtaaattt tcgacttagt tttcgaagaa gctatttttg tcacagcctc tctttttaag
     3841 tttagctatt agataatgat gtttagtttt ggataaacga aatcaattct tcacccgaca
     3901 aaccatagtt tacagcaatc aatagtttta aacgtgcttt ttgactatta atactgttac
     3961 agaaaataat ccccattttt tctagttctt ttcccccgcc ttcataatca taaacaggct
     4021 ctgcgattcc attgaaacaa cgagaaacaa gtactacagg gattttttgg acaagaagct
     4081 tttccaaagc agctaaggct tgtggaggga gattccccgc acctaaagct tcgattacca
     4141 aaccgtctac tttcgtatgg gcgatggctt ccaataagtc cccttgcatc ccagcatatg
     4201 ctttaacgat cgggatagtc ccatctacag cagaaatgtc cagtcgcttt gtttctaaaa
     4261 gttcctgcaa gaataaaatt cgattcttag ttactaagcc gatgggaccc aatgtaggtg
     4321 ttcggaaagt agcaacattt gtggtatggg tctttgtcac gtagcgtgca gaatggatct
     4381 catcgttcat gaccaccaaa acccccttat ctagcgcttc ttcacaactt gcgacacgaa
     4441 tcgcactttc aaaattataa aggccgtcac ttccaagttc attacttgaa cgcattgccc
     4501 cagtcaaaac aattggtaat ttgttgccaa tcgttgtatc aagaaaaaaa gcagtttctt
     4561 ctaatgtatc tgtaccgtgt gtgatcacca ctccggatat acctgatgca taagcggaca
     4621 agatacgttc ttttaatacc aacatgtgag aaggcatgat gtgtggtgag ggaagattga
     4681 agatatcttc aactaataat tctacatgct tggggattgt gatttctgca tctaataatg
     4741 ggtttgccac atcaggacta acaccgcccg tgagcatgtt ttctttcatg gcgatcgttc
     4801 cgccggtgtg tagtacaagt atttttttca ttttacagac ttccttacga ataattttgc
     4861 atagccgtta aatcttattg tatactatat aaaagagttt tgctatcagt gaagaataac
     4921 gaatagccta acttatcata aaaaagctga ccttattaaa atggaaaaga ctcaaccttg
     4981 acctcctaat acaatggagt atctggttga gtttctttta caagaaagca gccaaggaaa
     5041 tcaatcacga aggtggccgg aaatgatcag aatcggatta acatcgttta aagaacatga
     5101 caaattaact ggtaaaaaga ataacactct ttatgaatat gcagggtttc tacccttagt
     5161 ggaattagat actgcctatt atggaattcc ccgaaagtcc tcagttgaga attggataag
     5221 tgaagtacct gaatcatttc gctttgtagt caaagtctat caaggaatca gcggtcaagg
     5281 agactggaag ccttattatc agaccgaaga agagatgatt gcgcattttt tagatgttat
     5341 gtcgccaatg gtagatgctg gaaagatgtt ttgcttttta gtccaattcc cctcatcatt
     5401 caaatgtacg aaagaaaatg tccagtactt gcgcaaactt agaaagtggt ttgagggtta
     5461 tcctgtttct atagaattgc gggattattc ttggtacagc gatgaatttc ttgaacgtac
     5521 aagaaacttt atgtctagag ccgctttttc tttagccatc atcgatgagc cacagttgct
     5581 gaatacaacg attccttttg atccatttgt caccaatccg aactttgctc tttttcgatt
     5641 ccatggtagg aatgcgcaag gatggcaagc aaatgataaa gagtggcgta aaaaaagaac
     5701 gttatatcgt tattcagaaa aagaactgca agaactagca catgaagtca aagtagttga
     5761 ttctaagaca aaagaggtag gtgtgatttt taataataat tctggtggtg atgcggctga
     5821 aaatgcatta gcactgcaaa aaatattaaa tatcaagcca gatggactca atccaaccca
     5881 aatggatctg ttttaagggc ttattttatt caagaaaggg gcgaagtcta tgatcaaagc
     5941 tatttttttt gatattgacg gaacattggt caccaatcgt tcaaaagcgt tggaatcaac
     6001 aaaacaagca attgaatatg ctcggaaaaa cggcatttta tgcggtgtag caacggggcg
     6061 cagcccagta aagatcaaag aaatcattga tgaattggaa ttagatatgt atgtcgtgta
     6121 taatggtcaa ctggttttta ctgcagatcg cacgattatc gaccaccctt ttgaacaaaa
     6181 agtattggaa catattgttg aatttgcaga tgagaaccat cgacaaatcg tttttggtgc
     6241 gagaaatcgg ttagatggat caacgacgat gctattagga caatcgattt ttatcaagcg
     6301 actagtcagc tttttaccgc gtaaattccc ggtacgtctg atgaaaaaaa tcctccaggt
     6361 ttttagccct caccgacaaa aggatcgtta tgaaacgtta gagatattaa aagaaccaat
     6421 ttatcaatgt atattgctca gtccggagtc agaacagaaa aagttagaac atcgctttcc
     6481 cgagtgtacg tttcagcgtt caaatacgta tactgtagat atcatcccaa aaggtggttc
     6541 aaaattacta ggcatccaag ctttcgcaaa tgctgtcgaa attgaaatgg aagaaattat
     6601 ggcattcggc gatcattaca atgatatcga gatgctgaaa ggtgtaggaa ttggagtggc
     6661 catggggaat gctcagatcg aagtaaaaca tgctgcagac tttgtgactc aatcaaatga
     6721 agaagatggg atatacgaag gactgaaaca cttcggtctg atttattagt caatgaaaga
     6781 aggaaaaaat cagtatggaa gcagagacac cttttcaaaa agtagaaaca ttccacaaaa
     6841 cttttgatcc aagaagacca gagataccca cggcttttac agaaaatgaa gctcatacac
     6901 gtgctgcgtt caaaatagaa gaacttgttg aatttgttgc agctacttca cctaataaag
     6961 aacaactgca tgaagctgtt atgctgctgc acgaagcatt agataaagca gaagaaaaaa
     7021 tacttggtaa aacacagtgg ggaagtgatc cgctggttga acaagtagat gcactgacag
     7081 atatccttta ctttacttat ggttcttttt cattactagg aatagtgcct gatcgaatat
     7141 tcgaaatcgt ccatcaggca aatatgggga aattatttcc agatggaaaa cctcattatg
     7201 accctgtaac aaataaagtg ttgaaacctg aaaactggca gatggaatat gctccagaac
     7261 ctaaaatcgc ggcagaaatc caacgtcaga ttcaagaagc gattgctaag aagaaatccg
     7321 ctgatatagg catgtgcaca aatgaaaaag agtgatcaga gtttcactat ttttgttatc
     7381 tttatacgat ggaaaaacaa aagattttcg tttaagttag tgaatgcatg aaaatagtat
     7441 gctataatct agaatagatg tcttttgcac gagagacgta ttatttggat ttatgccaag
     7501 cagaacgcca tctgttgcat aaaagatgca tactgaaaac ggaggaacca taaatgggaa
     7561 ataatgttat catcggaatc gtcattgcaa tcattatagt cgccattgtc gtctttttgg
     7621 ttagttactt gatgaagaaa aaaaaccaag atcgattgga tgccttagaa aaacgcaagg
     7681 aagaattatt tgacttgcca gtgattgaag aagtcgatga tgttaaaaaa atgcatctgg
     7741 ttgggcaaag ccaaaatacc tttcgtgaat ggagtcaaaa atggaatgac atatctactt
     7801 cctcgttcgc tgaactagaa agtcaaattt tcgaagtgga aaacctcaat gaaagttttc
     7861 ggttcttaaa aggaaaaaaa gcagttgttg aagcagaagc tacaatggac caaatggaga
     7921 aacaagtgac ggaaatccgt gaaggtctaa aggaattgcg ggaaacggaa gaacgcaact
     7981 cactagctgt tcagcaggca cttgacgtct atgaagaact gaaagaaaaa gtcagagaag
     8041 acgagcatga atacggacca gctctttttg aattgcaaaa acagatcaaa gtgatcgaaa
     8101 aagaatttac gcagtttgtg actttaaaca cttctggaga ccctgttgaa gcccgcgagg
     8161 ttttggataa agcagaaaaa catacgtatg aagtggaagc tctgatgaaa aaaattccac
     8221 cgctgtatga ggagctccat accactttcc ctgaacaact tgaagaaatc agtgatactt
     8281 acgagaaatt gatggaagaa caatatgtat tcccagaaga agatcttgct gaagatatcg
     8341 caaaagtaag tcgaagaatt gaaaattcat tagccaatct tgaaaaaaca gaagttgaaa
     8401 cggtcgaatt tgaaaaccgt gaaacagctg atttgattga ttcgttatat gatatcttag
     8461 aacgggaaat ggaagctcag agatatgtaa aaactaacca atcaacgatt gcagagtata
     8521 tcaaacatac aacaaagaat aaccgtcaat tattgattga gctggatcac acatctcaaa
     8581 gctatacatt gaaccacaat gaaattggcc gtgtacgagg tttccaaacc gaagtggaag
     8641 aaatggaacg tcaaaatgaa cagatgattc cacagatccg tcaacacgaa attccttatt
     8701 cagaagtgcg cacgttctat aaaacagtct tcaaagtctt agaagatatc gaaaagcaac
     8761 aagtagagat cgacgactca ttacatgaat tgagaaaagg cgaaaaagaa gcgcaagaaa
     8821 agatcgatac attcgaattc aaattgcgca gtttgaaacg ttttgtagaa aaacaacgtt
     8881 tgcctggctt gccgaatgat tacctagaat tcttttttgt tgctactgac cgtattgaag
     8941 aactaagtgt tgtcttgaat aaaattcgag taaacatgga agaggtcaat cgtttagttg
     9001 ctctatgtga agaagatcta gaattattag ataagaagac acatgattta gtagatgcag
     9061 cagcattaac ggagcaaatg ctccaatatg caaatcgtta tcgtcataca cacgaagaag
     9121 tgagagaagc aattgataaa agctactatt tattcaataa agaatatcac tatcaagaag
     9181 cattagacga aatcggtaca gcattagagc gagtagaacc tggtgctttc aaacgcatcg
     9241 aagattttta ttttaatcat ccagacttag tataaagcaa aaaagttagt aaaccagcaa
     9301 gagcaagacg gattattgtc ttgttcttgt ctttttttgt atatttgaaa attgaaacaa
     9361 gtgaaaattt cacctgctct tgaaagcacc ttggacaacc gttataatag aaaagaattt
     9421 caaaaaagag gggtttctta tgatatattt tgataacagt gcaacaaccc caatctttcc
     9481 gcaaagctta gatgcatatg taaaaaccag tcagcggatt attgggaatc catcaagctt
     9541 gcatgattta ggcaatcaag ctaaccggtt gttgcagcaa gcaagaaagc agatcgctga
     9601 tttgatccat gtagaaccag aagaaatcta ttttacttca ggcggaacag aaggagataa
     9661 ctgggtatta aaaggaacga tgatcgaaaa aagagaatat gggaatcata tgatcatttc
     9721 aggagtcgaa catccggccg tcagcgagac agcagaacaa ttaaaagaat taggaatcga
     9781 agtaagtatc gccccagtgg ataaacgcgg atttgtacgt gtcgacgaat tgaaagaatt
     9841 aataagaaaa gagacagtat tagtctctgt tatggctgtg aataatgaag taggatcgat
     9901 tcagcccatc caggaaatca gtgatctttt ggatgatttt cctaaaattc atttccatgt
     9961 agatgctgtt caggcgattg gaaaagtacc attctcgcaa tggttgacag aaagagttga
    10021 ttttgcaaca ttttcagctc ataaattcca tggcccacgg ggaactggtt tcatttattg
    10081 gaaaaagggt cgtcgtttag caccattact gacaggtgga gggcaagaaa gaaatcagcg
    10141 aagtgggaca gaaaatgttc caggtattgt ggctatggtt aaagctttac gtttatcatt
    10201 agagataaag gaacaaaaac ctgagcagac acaacgctta aaagtagctc taatcaatgc
    10261 gttgaagacg tatgagaaag tgacgatttt ttcagaagga actgactatg caccgcatat
    10321 tttatgtttt gctctaaaag gaattcgcgg cgaagttctc gtccatgcct tagaagaaaa
    10381 acaaatattt gtttctacta ctagtgcctg ctcctcacgt aaacatatgg caagcagtac
    10441 attgcacgca atgcatgtac caaatgatct ggctacatct gctgttcgaa tcagcttaga
    10501 tgaaaacaat tcgcttgcag aagtcgagca atttatgatt attttcaagc agctttataa
    10561 gaaattttct gcaatcaatt aaaagaggag tgttcttgtg aaatacagtg aaattatggt
    10621 ccgttatggg gaactttcga caaaaggaaa gaatcgaaaa agctttatca tgcaactagc
    10681 acaaaatgtc agacaagctt tgaaagactt tccagaaatc aaaatccatg ctgatcggga
    10741 ccggatgcat cttttattga atggcgcaga cgggcagcta gttattccaa aactagcaaa
    10801 aatatttggg atccaaaatt tctctccaag tattcgtgta gaaaaagatg tcgaagtttt
    10861 gaaacaagct gttcaagaca tcatgaaaga aatttatact gggaaagaaa catttaaaat
    10921 tatcgctaaa agaagcgatc accaatttga attgacaagt aatgaattga atcaaacatt
    10981 aggtaatgct gtttttgata ttttcccgca tatccaagtt caaatgcgtc aaccagatat
    11041 tccattacgt gtagagattc ggcgtgatgg cgcttatcta tcgtatgaaa cgatcaaggg
    11101 agctggagga ttgcccgtag gaacaagcgg aaaaggaatg cttatgcttt ctggggggat
    11161 tgattctcct gtagcagggt actttgccat gaagcgggga gtggaaattg aagctgttca
    11221 ttttgctagt ccgccgtata caagtgaaca agcactgcaa aaagcaaaag atctaacagc
    11281 caaactagca ccttatgtgg gaacgatcca ttttatcgaa gtgccattta ccgagattca
    11341 agaagaaatc aaaaagtcag tgccacaagg atattggatg acaattactc gtcgaatgat
    11401 gttgcgtttg accgatgaaa ttcgccgtat tcgtcatggt ttagtcattt tgaatggtga
    11461 atcattaggt caagtggctt cacaaacttt gcaaagtatg gtggctatca atgaagtaac
    11521 gaatacgcca atcattcggc ctgtagcgac aatggataaa cttgaaatca ttgaagtagc
    11581 agaacagatc gatacattta atctttctat ccagcctttt gaagattgct gtacgatttt
    11641 tgctcctcca cagccaaaaa cacgccctca actggaaaag gtccatcaat atgaagagcg
    11701 tttagcaata gatgaaatga tccaacgagc actagctggc ttgaagatgg aagagataca
    11761 agcagaatcg tctaatgaaa aaactgaaga attttcagaa ttgctataag aggttatgaa
    11821 aatagtgttt tgaccagagt attagagaaa caaaacgaaa aaatagctcc atgtatttta
    11881 ccgtttttga actaatgtcg aagattagct gattgaatac catttatcaa gaagttatgg
    11941 aaaagccggt tcttttttac taactgaaaa ataaaatttt atttagagat caagctacag
    12001 ttgataaatg atataaaaaa gagaatttcc catgaaatag cgaggttgtg acagaagtgg
    12061 gcagcttcaa gaaataagaa ggaatttacg aaaattgctt ttcaaatttt tgtgaaattt
    12121 cggcttattt ttcgaagaag ttacttctgg aacaccgtgt atcagaggtt g
//
LOCUS       DACEUZ010000085        11932 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.085, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000085 DACEUZ010000000
VERSION     DACEUZ010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11932
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.085"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            51..260
                     /locus_tag="IXG44_001893"
     CDS             51..260
                     /locus_tag="IXG44_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1658610.1"
                     /translation="MICYISFLILGLPQAVFPEMFSGDWGIVSYSILTLIAMVLGTSF
                     YNAFVSKHVEEYEVLMYLKEKKTSN"
     gene            467..667
                     /locus_tag="IXG44_001894"
                     /pseudo
     CDS             467..667
                     /locus_tag="IXG44_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298761.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1154..1333
                     /locus_tag="IXG44_001895"
     CDS             1154..1333
                     /locus_tag="IXG44_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1658611.1"
                     /translation="MTELTADMSDTWLQEYLFPEKQTTARGYLPVDWEYVHKELMKKN
                     MTLKLLHREYGVHGT"
     gene            complement(1379..1984)
                     /locus_tag="IXG44_001896"
     CDS             complement(1379..1984)
                     /locus_tag="IXG44_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1658612.1"
                     /translation="MAKIGYIRVSSQDQNLDRQLAMMEDLNIDKLFQEKASGKDIQRP
                     EFQKLLNYIREGDCVIVASLDRLGRDYEDIKNTVTFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAKKAGRYKGRPIEYSENSTDPQ
                     KRLVFKTVVDMLNQGVPVAEIAKENGLSRPTIYKIKKANDL"
     gene            2166..2501
                     /locus_tag="IXG44_001897"
                     /pseudo
     CDS             2166..2501
                     /locus_tag="IXG44_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343406.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2666..2893
                     /locus_tag="IXG44_001898"
                     /pseudo
     CDS             2666..2893
                     /locus_tag="IXG44_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613886.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            3019..3990
                     /locus_tag="IXG44_001899"
     CDS             3019..3990
                     /locus_tag="IXG44_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1658613.1"
                     /translation="MKTTMKDVAALAGVGVGTVSRVINGVKVKEVTKEKVMAAISELN
                     YEPDEYARGMKTNSSNTIALILPTIWHPFFSEFAYYVEEALSQKNYKLFLCNANGSAE
                     KEKEYIQMVKQSKVDGIIGITYSDIDKYVSSNLPFVSIDRHFSEQVSYVTADNYLGGQ
                     IAAQELLTRNCKNLCYVGGVSPYPNETNNRKAGFYNICEENKIAVKVLDMPEPISNLE
                     EQLLAFFSDNPEIDSIFTINDFMALDVIKAQKKLGKEAPKDFQIIGFDGVKLSLQKDY
                     LLSTIVQPIEEMAKVSVETLMAMILNEEPPKRVVVPVFFHAGETTKE"
     gene            4130..5443
                     /locus_tag="IXG44_001900"
     CDS             4130..5443
                     /locus_tag="IXG44_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAQ1658614.1"
                     /translation="MKKRMLAIAAILALTAGGLAGCGSKEKSSGSSDPGEIKIWVQFS
                     DETPEGKAWAEIVNNFNDEYEGEYKAVTEYIPRSGSGGGYEDKVNAAITTNTLPDVIT
                     LDGPNTAAYAHSGVIAPLDDYLKDADMDDVLDSIIQQGTYDGKMYAFGFSESSVGVYY
                     NKSLFKDAGITEDQLPTLEDPWTWTEFQAICQKLTDKYKTPAIDMQMASGDEMLTYAY
                     TPFIWSNGGDVVNADGTKAEDYFNGKKSVEALEFIQELVEKGYTTISPIEKGFETGKY
                     AMMLSGSWTMADLEENYQEIDYGILPYPVSDATGELVSPTGSWQLAMTEKTEKKDASA
                     AFIKFATSTESSRIMSLGNSVLPIRNSTVDLIKDEVSEGMQVLIKQNQLSGHARPVVV
                     AYPQVSRAFQQAVQDISYFEENPDVQKVADSRAAEMQQAIDTSLK"
     gene            5486..6352
                     /locus_tag="IXG44_001901"
     CDS             5486..6352
                     /locus_tag="IXG44_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1658615.1"
                     /translation="MKDNLIGYGFLAPALLLLTIFLIIPVIMVIYYAFTDYYLLTPDA
                     RQFIGLENFKRLFADPIFVKSIWNTAKFVVWIIPVQIGAALGMALIINKNRKGNIFFK
                     VAFFAPVVMSLVVISILWLYLLNPNEGLVNTLFAKIGIASQPFLTSPKQAMYTLVFVS
                     AWQGAGYQMLLFLGGMQNIPQDVYEAAEIDGFSKFQQFRYITMPLLKPTAIFILLTTL
                     ISAFKLIIQPMVMTQGGPMNSTMTMVYYIYQTGFTDRMVGYSSSIALIFTTMIGLITI
                     AQRKLVKEDDPA"
     gene            6349..7197
                     /locus_tag="IXG44_001902"
     CDS             6349..7197
                     /locus_tag="IXG44_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006639650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1658616.1"
                     /translation="MTKKFGPLMIVEYCLLFLLAALFIFPMLWMVVSSMKPEADVYQN
                     LASFKAFLPSLNPANWFKTYQEVISRFSVGTYLLNSIFYGLTFACGSIIVNSLAGFAF
                     AKVNFSGKKIIFGALLALLIVPMETVLIPQFTIVNSLGLVNTRLAVILPAMASAFNIY
                     LFRNFFIAIPEEIIESAKLDGANIVKIFFHIMLPMSKPAIATVGVLSFISSWNDYIWP
                     LMVLTDSSKFSMQVAITTINTTQPVYINQVMAVLTIATIPLIIVYIVAQKYILQGLGG
                     SGTGIK"
     gene            7211..8683
                     /locus_tag="IXG44_001903"
     CDS             7211..8683
                     /locus_tag="IXG44_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HAQ1658617.1"
                     /translation="MLQKLLKANEFIEKNAQTVNQEYRNKFHLMAPIGWMNDPNGFVY
                     YQEEYHLFYQYYPYDSIWGPMHWGHAKSKDLIHWEHLPVALAPGESYDKEGCFSGSAI
                     EKDGKLYLIYTGHNVVEGQVRQVQCLAVSEDGIHFEKYAGNPIIAEDHLASVATTEDF
                     RDPKVFQRKEDYFTVVATKTADARGQIVMFHSKDLLTWEFYSVLLEGTPEQGIVWECP
                     DLFELDGKEVLILSPIQMTPQGNAYQNTNSTVAFIGTVDWQTGKFLVENFHEIDGGLD
                     FYAPQTCENEAGQRIMIAWMQMWQRKIPTNDLGHGWSGSMTLPRELRVADNQLLQRPV
                     SSIYQVIDPQERIEWAKTEENRFLLKDLVYDQHYLRLEINLKQATKLELHYAKGPDHT
                     LLLNYDVERECFTASREHMPYDLSGAEKEVLKKRSVTIPLENNCLTIEIFRDTSAIEL
                     FLNDRLTMSTTFYETEKGQDFLLLADSEVTFSLESGLINL"
     gene            8700..9572
                     /locus_tag="IXG44_001904"
     CDS             8700..9572
                     /locus_tag="IXG44_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ1658618.1"
                     /translation="MIYGSIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETIPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKVAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIEKRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
     gene            complement(9808..11190)
                     /locus_tag="IXG44_001905"
                     /pseudo
     CDS             complement(9808..11190)
                     /locus_tag="IXG44_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010726506.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            11652..>11932
                     /locus_tag="IXG44_001906"
     CDS             11652..>11932
                     /locus_tag="IXG44_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1658619.1"
                     /translation="MDSFQRLSLVNQFELLAKFEDQNRSYYERKIEILREGYEYHYDD
                     EIWSDLSEPFPKEDSRFVLDVLNMYRDINFSKSKLNLDENQITNSYYTH"
BASE COUNT         3700 a         2038 c         2402 g         3792 t
ORIGIN      
        1 atttaaaaag aaaagtgaca gataggaata aaaacttatt aaatgtagtt atgatctgtt
       61 atatttcatt tctaatacta ggtcttccac aagcagtgtt tcctgaaatg ttttctggag
      121 actggggtat tgtcagctat tctatattaa cattgattgc tatggtttta ggtacttcat
      181 tttataatgc ttttgtaagt aagcatgttg aggaatatga agtattaatg tacttaaaag
      241 agaaaaaaac aagtaattaa tatgagaaca ccacgcatag tttttatttt gagatattct
      301 agaatgaaac acattaagaa cgaaagcaaa gcaaaagacg ccagaacttc tactaccttc
      361 taagtttttt catatttgtt tgttatgcta tacatagatc tgcataatta gacttgcgct
      421 gcccgttatt gaagtttcag gcagcgctct ttttggggga tcgataatgg aagaagaaaa
      481 aatatttgag aaacgctggc agctagcatc aagtgaacaa agagcaagat acaataattt
      541 gatcacctcc taccccactg ttgactagac atataaggaa aagaaatact tactttggct
      601 gtgccagtta gatatagata cctttgaaac atttgaagtg atcctagata aaattaaaca
      661 gaattgatca gaaacaagcg aagccttctc tcggttcgct tgttttgcta tttattagtt
      721 ggctcagggc aactctaagc tcgtttgaaa gaaaaccgat ggataatccc cttgtttgta
      781 ccactttaga atacatggat gtacgtattg ccattgactc ctattagtta ctgaaaaaaa
      841 atttaaacaa gttgagtatt gcagataaat agctccagtg aggtgaataa ctgctccact
      901 caaggcaata gatagctcca tccagggcaa caaactgctc cactttcata tttaatagat
      961 gaataacctt ctgtataata gaaaggcata cctttcggta tgacaactta tacagaatgt
     1021 tattttattg gttcactatc gaaaaatttt ggagctttat ttttccggta tttctcaacg
     1081 aacgattagt ttaactgtgg gaagctccag acatacgatt cgagaagtta cttctcgagc
     1141 agacaggaag ggggtgactg aactaaccgc tgatatgagc gatacttggc tgcaggaata
     1201 tctttttccg gaaaaacaaa caacagcacg aggttacctt cctgtagact gggaatatgt
     1261 tcataaagaa ctaatgaaaa agaatatgac attgaaactc cttcatagag aatatggggt
     1321 acatggaaca tagaaacgaa aatgcacggt agaaaagcga atgaatatag tataaattct
     1381 acaaatcatt cgctttcttt attttatata tggttggtcg agagagacca ttttctttgg
     1441 caatttctgc aactggaact ccttgattta acatgtccac cacagtttta aagactaatc
     1501 gtttttgcgg atctgttgag ttctcagaat attcaattgg tcgcccctta tatcgaccag
     1561 cttttttggc caataatact ccttgccgct gacgttcacg gattttctca cgttcgtttt
     1621 gtgcaatata acttagcaat gataaaaaca tatcgaacat agcagtatct aggagttcat
     1681 ttccagtatt aaaatctaaa aactgtgcat ctagtatttt taatgctact tttttctgtt
     1741 tcataaaggt aactgtattt tttatgtctt cataatctct gcctaggcga tccagtgatg
     1801 caacaatgac acaatcacct tctcggatat aatttagtaa tttttgaaac tcaggtcgct
     1861 ggatatcttt accactggct ttttcttgga ataatttatc aatgttaagg tcttccatca
     1921 ttgccaattg tcgatctaaa ttttgatctt gtgaagacac tctaatatat ccaatttttg
     1981 ccataaaaac tccttttaaa aaatgatata atttacatgt aaaatttact tgtagtttta
     2041 ctgttatcat agcattttta aaaataaatt ttaagtctac ttataattta ctttggaggt
     2101 tttgaatgaa tgaaattaag cgcaataaaa attcttttgg tgtttattta ggagagccta
     2161 gctcggaaca gttaaataaa ggtgaagctc gtcatgctct atgtcgaaat atattttatg
     2221 gtagaaaagg caaactttat caaacttatt acgacggtat ggaggaacag ctcaacgcat
     2281 taggattagt aactaatgct atcatatatt ggaattcact atatttagaa aaagtctttg
     2341 aacaaattaa aacggaaggg ttcgactgtt ccgaagaagt gttacgcaag caatttccat
     2401 taattagtga tcacattaat tttgttggca aatatagttt ccgctatgaa aaagaattat
     2461 cgaatggaat actacgtccg ttaaatatag agaattattg agaatgttcg tcttttggtt
     2521 cctaccgtgc attttcgcac ggatgttcca gatactattt ttgaatgtat aaaattgaaa
     2581 acacaagaat actaataaat caacgattct tgtgttttta atttttcact accgtgcatt
     2641 ttcgtttcta tgttccatgt accccaatct tgaaaaatta attaaagact tagataaagc
     2701 cattgacgac ttagtcgtgg tgctaccgga gtatcaatgt ttaaccagta tcccaggtat
     2761 tggtcctgtt taggctgctg gaatactcgc tgaaattggt ccaattgaac gtttttctga
     2821 tgaaataaaa ttgacaaagt atgccggcct atattggcga cagagtcaat ccggtaaatc
     2881 tgaatacgaa aattttttta cgaaaagctt cacgtctaat atctatttta aaataaaaat
     2941 attcctgcta atttggaatc gtttccttct tttggtataa taatacctga aattcttttg
     3001 agaggagttg ttcgtgaagt gaaaactact atgaaggatg ttgctgcgtt agcaggagtt
     3061 ggtgtgggta cggtatctag agtaataaat ggagtaaagg tgaaagaggt taccaaagaa
     3121 aaagtaatgg ctgcaattag tgagcttaat tatgagcctg atgaatatgc cagaggtatg
     3181 aaaacgaata gtagcaatac aatagctctt attttaccta cgatttggca tcccttcttt
     3241 tccgagtttg cctactatgt agaggaagct ctcagccaaa aaaattataa gctttttttg
     3301 tgtaatgcga acggcagtgc ggaaaaagaa aaagaatata tccaaatggt caagcagagt
     3361 aaagtagacg gaattatagg tattacctat tcagacattg ataaatatgt atcatcaaat
     3421 ttaccatttg tcagtataga ccgccatttt tcggaacagg tttcttatgt aacggcagat
     3481 aattatttag gtgggcaaat tgccgctcaa gaactactta ctcgtaactg taaaaacttg
     3541 tgctatgttg ggggtgtttc accttaccct aacgaaacaa acaatcgtaa agctggtttt
     3601 tacaatattt gtgaggaaaa taaaatagct gtgaaggtat tggatatgcc tgaaccaatt
     3661 agcaatttag aggaacaatt gctcgctttt ttttcggata atcctgaaat tgatagcatt
     3721 tttaccatca atgatttcat ggctttggat gtgataaaag cccaaaaaaa actagggaag
     3781 gaagcaccta aggattttca aattattggt tttgatggag tcaagctatc gttgcaaaaa
     3841 gactatttac tatcaacgat tgtacagccg atagaggaaa tggccaaggt ttcagtagaa
     3901 acactcatgg cgatgatttt aaatgaagaa ccgcctaagc gtgtggtagt accggttttc
     3961 tttcacgctg gagagacgac taaagagtaa aaaaccggtt tataacgatt tttttgagat
     4021 aggttgacag aaagtgattt caatgatatc ttaaaaccaa ttaattggaa acgttaccaa
     4081 ttaattgaaa aataaaacgg aaacgtttca aaaaatagga ggaacatcga tgaaaaagag
     4141 aatgttagca attgcagcaa ttttagcatt aacagctgga ggacttgcag gatgtggcag
     4201 taaggagaaa agttcaggta gcagtgatcc aggagaaatt aagatttggg ttcagttttc
     4261 tgatgaaacg cctgaaggaa aagcttgggc agagattgtg aacaatttta atgatgaata
     4321 cgagggtgaa tataaagctg ttactgagta tattcctcgt agcggaagcg gcggtggata
     4381 tgaggataaa gtaaatgctg caattacaac caataccctt cctgatgtta ttacgttaga
     4441 tggaccgaat actgcagctt atgctcattc aggagtcatt gctccattag atgattattt
     4501 gaaagatgcg gacatggatg atgtattaga cagcattatt cagcaaggaa cctacgatgg
     4561 caaaatgtat gcgtttggtt tctcagagtc cagtgtgggt gtctattaca ataagtcgct
     4621 gttcaaagat gctggaatta cagaagatca attgccgacg ttagaagatc cttggacttg
     4681 gacggaattt caagcaattt gccaaaagct gactgataaa tacaaaacgc ctgcaattga
     4741 tatgcaaatg gcgtcgggag acgaaatgct gacctacgca tatacaccat ttatatggtc
     4801 gaatggcggc gatgtcgtta atgctgatgg aaccaaagcc gaagactatt ttaacggtaa
     4861 aaaatccgtt gaagcgttgg aatttattca agaattggtt gaaaaaggtt acaccacaat
     4921 cagtcctatt gaaaaaggat ttgaaactgg caaatatgcc atgatgctga gcggttcttg
     4981 gacaatggca gatttagagg aaaactatca agagattgat tatggtattt tgccttaccc
     5041 cgtttcagat gcaactgggg agttagtttc accaaccgga agctggcagt tagcaatgac
     5101 tgagaaaaca gagaaaaagg atgcttctgc ggcctttatt aagtttgcaa ccagtacaga
     5161 atctagtcgt attatgagtt tagggaacag tgttctacct atcagaaatt caacagttga
     5221 tttaattaaa gatgaagtgt cggaaggaat gcaggtatta attaagcaaa accaactttc
     5281 tggacatgcc cgcccagtgg ttgtagctta tccgcaagta tctagagcat tccaacaagc
     5341 agttcaagat attagctact tcgaggaaaa cccagatgta caaaaagtag cagatagtcg
     5401 cgctgctgaa atgcaacagg caattgatac ttcactaaaa tagaagtttc taaagggggc
     5461 caatcaagtg ttaaaaaaac cgacaatgaa agacaatttg ataggttacg gctttctagc
     5521 gccagctttg ctgctgctga cgatttttct gatcataccc gtaatcatgg taatttatta
     5581 tgcctttact gattattatt tgttaacgcc agatgctaga caatttatcg gcttagaaaa
     5641 ttttaagcga ctgttcgctg atccaatttt tgtaaaaagt atttggaaca cggctaaatt
     5701 tgttgtttgg attattccgg tccaaatagg ggcagcttta ggaatggcac ttattatcaa
     5761 caaaaatcgt aaaggaaata tcttttttaa agtagccttt tttgcgccag tcgtgatgtc
     5821 cttagtcgtt atttcgattt tatggttgta cttgttaaat cctaatgaag gtctggttaa
     5881 tacgctattt gcaaagattg gaattgcttc gcagcctttt ttgactagtc ccaaacaagc
     5941 aatgtatacg cttgttttcg tatcggcatg gcaaggtgca ggatatcaga tgctgctgtt
     6001 tttaggggga atgcaaaata ttccgcaaga cgtttatgag gcagccgaaa ttgatggttt
     6061 ttctaagttt cagcaatttc gctatatcac catgccatta ttaaagccca cggcgatttt
     6121 tattttactt accacattga tttcggcctt taaactaatc attcagccaa tggtaatgac
     6181 tcaaggtgga ccaatgaatt ctactatgac aatggtttat tacatttacc aaactggatt
     6241 tacagaccgt atggtaggct actccagctc cattgcgtta atctttacca ctatgattgg
     6301 tttaattaca attgctcaaa gaaaattggt aaaggaggat gatccagcat gaccaagaaa
     6361 tttggaccgc taatgatcgt agaatattgt ttgctgttcc ttttagcggc attattcatt
     6421 ttcccgatgc tgtggatggt agtttcctct atgaagcctg aagcagatgt ctatcaaaat
     6481 ttagcttcct tcaaagcttt tttacctagt ttgaatccag ctaattggtt taaaacttat
     6541 caagaggtta tttcccggtt cagtgtggga acttatttat taaacagtat tttttatggt
     6601 ttaacctttg cctgtgggtc aatcattgta aactctttgg cgggttttgc ctttgcgaag
     6661 gttaattttt caggcaagaa aattatcttt ggagctttgt tagctctgct gattgtcccg
     6721 atggagacgg ttttaattcc gcaatttacc atcgttaatt cactaggctt agtgaatact
     6781 cgcttggcag tgattttacc agcgatggcc agcgcgttta atatctatct gtttagaaat
     6841 ttctttatcg cgattccaga agaaatcatt gaatcagcga aattagatgg tgcaaatatt
     6901 gttaaaattt tctttcacat catgctgcca atgtctaaac cagccatagc cacagtaggt
     6961 gtcttatcct ttatctccag ctggaatgat tatatttggc cgttaatggt cttaactgac
     7021 agcagcaaat tttcaatgca agtggcaatt actacgatca atactacaca gccagtttat
     7081 atcaatcaag tgatggctgt tttaaccatt gcaacgattc cactgattat tgtttatatc
     7141 gttgcacaaa aatatattct gcagggactt ggcggttctg gaacgggtat aaaataggag
     7201 tgaatagtga atgttgcaaa aacttttaaa ggccaatgag tttattgaga aaaatgctca
     7261 aacagtcaat caagaatacc ggaataaatt tcatttgatg gctccgattg gctggatgaa
     7321 tgatccaaat ggttttgttt attatcaaga ggagtatcac ttgttttatc aatattatcc
     7381 ttacgacagc atctggggac caatgcattg gggccatgct aaaagcaagg atctaattca
     7441 ctgggagcat ttgccagtag cactggcacc aggtgaaagc tatgacaagg aaggctgttt
     7501 ttccggcagt gccatagaaa aggatggcaa gctgtatttg atttatacag gacacaatgt
     7561 tgtggaaggg caagttcgac aagttcagtg tttggcagtt tcagaagatg gcattcactt
     7621 tgaaaaatat gccggaaatc caatcatcgc agaagatcac ttagcttctg ttgcaacgac
     7681 cgaagatttc cgcgatccca aagtgtttca gcgaaaagaa gactatttta ctgtagtcgc
     7741 tactaaaact gcagatgctc gtggacaaat tgtgatgttc cattccaaag atttactaac
     7801 ttgggagttt tattccgtat tgctggaggg aactccagaa caaggcattg tatgggaatg
     7861 cccagacttg tttgaactag atggcaaaga agtcttgatt ctttctccta ttcaaatgac
     7921 tccacaaggc aatgcttacc aaaatacgaa ctcaactgtt gctttcatcg gtactgtcga
     7981 ttggcaaaca ggcaaatttc tagtagagaa ttttcatgaa atcgatggcg gtttagattt
     8041 ttatgcaccc cagacctgtg aaaacgaagc aggccaaaga ataatgattg cttggatgca
     8101 aatgtggcag cgtaaaatac ctacgaatga tctgggccat ggttggtcag gttccatgac
     8161 cttgcctaga gagctgaggg tggctgataa tcaattgttg caacggccag tttcatcaat
     8221 ttatcaagta attgatccgc aggaaaggat tgaatgggcg aagactgaag agaatcgctt
     8281 cttactgaag gacttagtat atgaccagca ctatttacgc ttggaaatca atttaaaaca
     8341 ggcaacgaaa ctagaacttc actatgcgaa gggacccgac catacattac tgcttaacta
     8401 tgatgttgaa cgggagtgct ttactgcttc cagggaacac atgccgtatg atttatcagg
     8461 agctgaaaag gaagtgttga aaaagcgcag tgtgacgatt cctttggaaa ataattgtct
     8521 aaccatcgaa attttccgtg atacttcagc tattgagctt tttctaaatg atcgtctgac
     8581 tatgagtaca actttttatg agacagaaaa aggacaggat tttcttttgc tggcagacag
     8641 tgaagtaacc ttttcattgg aaagcggctt aattaatcta tagaagggag agctcagtga
     8701 tgatttatgg ttcaatcgaa gcaggcggca caaaatttgt gtgtgccatt ggcgatgaag
     8761 aaatgacgat caaggaaaga gtgagctttc cgacaacgac acctgaggaa acaatacctt
     8821 tagtcattga cttcttcaaa caataccaag cagatttagc agggattggg attggctcat
     8881 ttgggcctat cgatatccat cgcgattccg ccacttatgg gtacattacc tcgaccccga
     8941 aagtggcatg gcaaaatttt gatttcatcg gtaccatgaa aaaagaattt ccgatcccga
     9001 tttcttggac aacagatgtc aatgcggctg cctatggtga gtatgtcttc ggcagtggca
     9061 aaggattgag cagtgtcgtt tactatacga tcggtacggg tgttggcggc ggtgccttac
     9121 aagaagggcg ctttatcgaa gggtttagtc atccagaaat ggggcatatg ctcgtcgtcc
     9181 ctcatccaaa agatgacttt gctggcagct gtccatttca tggcaactgt ttagaaggga
     9241 tggccgctgg acccgccatc gaaaaacgct tagggaaaaa aggacaagag gttgcagaag
     9301 atgatcctta ttgggaaatc gaagcctcct atattgctca atgtgcctac aatacaacct
     9361 tgatgttttc tccagatgtg atcatttttg gcggtggtgt gatgaaacaa cgccatatgc
     9421 tgcaaaacgt ccacgacgca tttgcaaaat tggtcaatgg gtatgtcgaa acacctgagt
     9481 taagcaaata tatcgtgacc ccagcattag aagacaacgc tggaacgctc ggttgtttgg
     9541 cattggctcg agaagcggtc ttacattcat aaacgaaaaa aggctcttcg acatttagtg
     9601 ttggtagaga aagatacaac tgaaaattta gtcataacca gttaggcaga gtgcttaact
     9661 ggtttttgtt ttacttggaa gatcaagcct tgaatgagat aagttcttgc tcgaaagtta
     9721 tggaaatttc gatagccgta ggctacttgt attgtgcaat agttttttac aaaaaaaagg
     9781 gggtgcggat attttttttg gaccttttta acaaatatat ctttgcttct tccgatattg
     9841 aagagggctt agccttccta aagataactt aattcgttga ttattatacc aatgaatata
     9901 atcatccaat ttgtttataa actgattgat tgttgtattc ttccagtcac gttgataaaa
     9961 catctcgttt tttaatcgtc caaagaaacc ttcgcacgct gaattatctg gtgaataact
    10021 ctttttagac atagagtgaa gcaagttggc tttgttccaa tagtccaact aacaatagca
    10081 ccatcgtaac aatcgacaag tggtgataag tagattttcc ctgcgagaat tctaaattca
    10141 gtaatatctg ttaaccattt ttcatttggc ttctttgctt taaaattccg atttaataaa
    10201 ttggggtgtg caggagaaat ttccccagca tacgaagaat acttttttcg tctagaaaat
    10261 ttaacaaata gattttcctc agtcatcaat ctacgtacaa ctttttcaga aagcttttta
    10321 cccatatttt ttaaagcttg gtgaattcta cggtaaccat acgcacagaa gttatgattg
    10381 aaaatcgtga taattaattt gacgttcttt gtaatactta tctggtttct caagtgcttg
    10441 tttttgataa aaatagctgc ttttaggaaa ctggagctgt tgaagaagct ctttcaaggt
    10501 gaactgattt cgtaaggcat caatcaatat cgttttttct tgatttgata gacaaagaag
    10561 attgacgcct ttttcttttt ttagcagttc agccgctcct tcgagtaatg ctttttcgag
    10621 ctgtaattgg tgtacttgtt tttgtaagtc ttctacttcc tttaaaagta gctctttttg
    10681 tttttcctga ttttttaaag tcatacacaa tggaaaatcc tttccaagaa ccgtgttttt
    10741 ccattgatat agagtaactc tactaattcc tccagattcg gctaaatttt tcctattagt
    10801 ttgcggttta tagaattttt tcaacacagc ttctttttga tcttgtgtta actttaccgc
    10861 actttttcgg agttttctag ctcctggtgc aagttcttca caccgctgcc gcaaggactc
    10921 tctattagga taaccaagca tgcgaatggt tcgtgcataa cattgaccat attcaaaata
    10981 atgattcacc gctattcgtt tttgttcttc agaatacttt gatttttttc ggtgctcctg
    11041 atggagctct ccggattcca aatactcttt ataccatttc cgtagtgcac caatcgacgg
    11101 ataccctaat tctcgaatgg ttgcagcata agagcaatcg tattttaaaa ataactgaat
    11161 tgcccttaca cgctctttgt gggaaaacac acggacattc ctcctaagtt tattttttcg
    11221 tccaggatta tgtccgcacc cccttgcttt ctgatgactt attctggttc tttgagtgtc
    11281 aatgcataga tgtgatgtag ctagttagaa aaaaattttg aatactcttt ttttctttca
    11341 gactcctttt taagtaagat tttgaagtgt gagttacggg gtcagaaact taaaagtttt
    11401 tgatccttta ttttcaagtc tgatttggca gcttttcggc agctcattta tttatggtca
    11461 gaaatagatg ctgaaatttt aatctattag caaatagtcc agaacgtatg aagaggagtc
    11521 ggatatcatt tgtcattaac accataaata tttataaaat tcgtgttcac ctacaattaa
    11581 atttaatata taatattcgt tgtaagggct tataattttt tgttaatgaa caagatagga
    11641 agaaacattt aatggacagt tttcagagat tgtcactagt aaatcaattt gagttattgg
    11701 ctaaatttga ggaccaaaac agatcttatt atgaaagaaa aatcgaaata ttgagagaag
    11761 gttacgagta tcattatgat gatgaaattt ggagtgatct ttctgagcca tttccgaaag
    11821 aagattctcg atttgtatta gatgtattga acatgtatcg agatatcaat tttagtaaat
    11881 caaagctaaa cttagatgaa aaccaaatta ctaattcata ctatactcat tt
//
LOCUS       DACEUZ010000086        11587 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.086, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000086 DACEUZ010000000
VERSION     DACEUZ010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11587
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.086"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            104..967
                     /locus_tag="IXG44_001907"
     CDS             104..967
                     /locus_tag="IXG44_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ1658620.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1096..2718)
                     /locus_tag="IXG44_001908"
     CDS             complement(1096..2718)
                     /locus_tag="IXG44_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1658621.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            2967..3488
                     /locus_tag="IXG44_001909"
     CDS             2967..3488
                     /locus_tag="IXG44_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1658622.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(3674..5404)
                     /gene="spxB"
                     /locus_tag="IXG44_001910"
     CDS             complement(3674..5404)
                     /gene="spxB"
                     /locus_tag="IXG44_001910"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HAQ1658623.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5569..6387
                     /locus_tag="IXG44_001911"
     CDS             5569..6387
                     /locus_tag="IXG44_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005232914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HAQ1658624.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6490..6687)
                     /locus_tag="IXG44_001912"
     CDS             complement(6490..6687)
                     /locus_tag="IXG44_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1658625.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            6856..7434
                     /locus_tag="IXG44_001913"
     CDS             6856..7434
                     /locus_tag="IXG44_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAQ1658626.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            complement(8479..8982)
                     /locus_tag="IXG44_001914"
     CDS             complement(8479..8982)
                     /locus_tag="IXG44_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAQ1658627.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            9340..10365
                     /locus_tag="IXG44_001915"
     CDS             9340..10365
                     /locus_tag="IXG44_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ1658628.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            10688..11566
                     /locus_tag="IXG44_001916"
     CDS             10688..11566
                     /locus_tag="IXG44_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1658629.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
BASE COUNT         3589 a         1973 c         2257 g         3768 t
ORIGIN      
        1 tgattagaac ttgcgaaaat aaaccggtta aagtgtgtct agcgcaagag atttgctata
       61 atagacgata agggtaaaat gtaacgagaa ggggttgaaa aatgtgtatc aaattgtaac
      121 agattcttgt tgtgatctgc cataccatgt attagcaaaa gaaaaagttg attttttatc
      181 aatgaatctc caattgaatg atcaagagtt agtagatgac ctcggaaaga cattcaatta
      241 tgaccgctac atgaatcagc tgaaaaatgg tgcgatgcct acgacatcac aaatcaatgt
      301 tggccgatat ttagaatttt tccgttctta tgcggaaaaa cagattcctg ttttatatgt
      361 ggctttttcc agtggattaa gcggctctta cagtagtgcg cttcaagcgg ttgaattgct
      421 gaaggaagaa tatgaagacc ctgagatcta tgtttttgat acaaaagctg caagtattgg
      481 ggaagggctt ttagtattgg aagctgtaag attgaaaaaa gaaggtaaaa gcatcgaaga
      541 aaccctagag tggctggaaa gtaattattt gaaagtccat tcatgggtaa cagtagacga
      601 tctgaaatat ctagaacgag gcggtaggat ttccaaagca agtgctactc ttggcagtct
      661 attgagtgta aagcctatta ttacggtgga taaagaagga aggctgacga atgttgacaa
      721 agttcgagga agaaacaagt cgatccagac aatcgtcaat gagacggtaa aaacaatcgt
      781 tgcaccactt gatcaaatcg tttatgtggc ttacgctggg gatctagaga gcgcggagaa
      841 ggcaaaacgg cttttggaag agcagataaa gatgaaagat gtgaagcttt atcccttagg
      901 accaacgatc gccagtcaca caggctatgg ctgtatcgcg attttttcga tgggaatcag
      961 tcgataaaag cagtaactca ttctgtaaaa aagcccagaa attgattgta acaaaaacag
     1021 cgactcttaa aagatagatg agcatcctat ctcttagagg tcgctgtttt tttatttttt
     1081 aagctaaata ggccattatg cccataactc gttcaattgt ttttgaatat ctgcattttc
     1141 caaaaattca tcatatgtcg tatcagctcg atcgatcaca cctttatccg aaacggcaat
     1201 gatgcggttt gccaatgttt ggataaattg atggtcatgt gacgcaaata agattgagcc
     1261 agtaaatgcc atcaagccgt cattcaatgc tgtgattgat tctaagtcta aatggttcgt
     1321 tggatcatct aatacaagta cgttagcttt cgaaagcatc atttttgaaa gcatgcaacg
     1381 gactttctct ccaccagata aaacattcac tggtttcaat acttcttcac cagaaaacaa
     1441 cattcttcct aagaagcttc ttaagaaggt gttatcatct tcttctttac ttgcatattg
     1501 acgcaaccag tctaaaatcg ttagatcagt atcgaattct ttcgttgtat cctttggtaa
     1561 ataagaatta cttgttgtca caccccaacg tactgttcca gtatctggcg taatttcacc
     1621 catgataact ttgaatagcg ttgttgttgt tatatcattt tctgcgacga aagcgacctt
     1681 gtcttctttg ctcaaattaa aagatatatc atctaaaatt ttctttccat caatcgttac
     1741 acttacattt tctacctgca ataagtcgtt tccaatctca cgttcaggtt tgaatccaac
     1801 gaatggataa cgtctagaag aaggttggat atcgtctaaa gtaatttttt caagcatttt
     1861 tttacgtgaa gttgcttgtt ttgattttga tgcattcgca ctaaagcggg caataaattc
     1921 ttggagttct ttgatttgtt cttctttttt ggcatttgcg tttgcttgaa gtttagccgc
     1981 tagttgacta gattccaacc aaaaatcata atttcctaca taaagtttga ttttaccaaa
     2041 atctagatcc gccatatgtg tacatacttt gttcaaaaag tgacggtcat gggatacaac
     2101 gatcacagta ttttcaaaat tgatcaaaaa ttcttccagc caattgatcg attggatatc
     2161 cagaccgttt gttggttcgt ctagcaaaag aacatcaggt ttaccaaata gtgtttgtgc
     2221 taataacact tttacctttt gtccaccagt taattcactc atttgctgat ggtgcaattc
     2281 ttcagggatg ttcagacctt gcagtaaaac agctgcttct ggttcagctt cccatccatt
     2341 aagctcagca aattctcctt ctaattcagc tgctcgaata ccatcttcgt cagtgaaatc
     2401 ctctttcatg taaatcgcat ctttttcttt cagcacttca tacagacgct tgtgtcccat
     2461 gatgactgtt tcaagtacag tatagtcttc atagtcgtaa tggttctgtt tcaatacggc
     2521 catccgttca tctggtccca tagtaacgga acctgtggaa ggctgcagat cccctgataa
     2581 cactttaagg aacgttgatt tacctgcacc atttgcacca atcaagccat agcagtttcc
     2641 gggggtaaat ttaatattca catcatcaaa tagtttgcgg tctgaaaagt gcaaactcac
     2701 atcatttaca gtaatcaatt tttttcctca cttttatcat tattagtttt gacaagtctt
     2761 atagaattat ataggggaaa ggtaaaagat tcaaggcaat cgaacattct ttgtgtttca
     2821 cgtgaaacag aatgaaaaaa ataaaaataa aagcggagcg aaaatagcta ctataggatt
     2881 tattagtttg caacagcata atttttgacg ttatttataa gagggagtac tataaaactg
     2941 taaaagaaat gaaagaagga ttgaaaatgg atgaaaaaag aaccatcgac tggggctcat
     3001 tgattttggg aattttattt gtacttgtct cattgttatc atttcaagac cctgtcggaa
     3061 atttagttgc aattgtagta gtctttgcga tttttgcctt tattaaaggg atatttgaac
     3121 tgtttgtacg gaacagatta aaagagctaa caggatataa aggaaagatg cccttaataa
     3181 tcgggatcat cgatattcta gttggcgtct ttttcttatt caatattggt gcaggagtcg
     3241 cagcattgcc ttttgttttt gcagtatggt ttattgctga ttcagtttta gcgttattta
     3301 ctgctgactt ggcaagagga gtaagtgaag gatattattg gtttacgatc atcgtcaata
     3361 ttttaggaat tttattagga attttcttat tgtttaatcc aatttcttct gcgttgacat
     3421 taagcttttt agttggattc tattttatgt tatttggtat cacacatatc gtctatgcat
     3481 ttagataagt aaaatcaaac aaataaaaaa gccggcttcc gattacagtt ttactgttgt
     3541 aattggaagc cggctttttt atcatggaca aatatgcacg aggtgatttt taggtgtcga
     3601 gacaaattta tgcaaaaatg tgagaagcta tatacacaaa ttttatctta atactcgaag
     3661 catagcgcag ttgtcacaac ctcttttcat ataattcatt caatactggc aaatcatgga
     3721 cttcatattt ttctttgaat gcttggattt cttcttcaga atattttctt ggatctaaat
     3781 gcagatcttc tactggcaat gggcgcttgt tattgatctt cacatcgatt actacaggac
     3841 gatctgattt tgcagctgca tcaaaggcaa atcgaagttg cgagtattct gtgactgtaa
     3901 agctatttgc cccgagcgct tctccaactt taccgaaatc tgcatcttct aaaaatacac
     3961 caaatttctt ttgttccgta tcttcttgtt ctgcttcgat gaaaccaaaa gaatcattcg
     4021 aaaagacaac attaataatt ggaagctgat atttgacttg tgtaatgata tcttgcatga
     4081 ccatagcaaa cccgccatca ccacttaagg taaatacttg tttttctggg tagcttagtt
     4141 gtgcggcaat tcctcctggc acaccattac ccatagttgc gaaccatcca gatgtcgtat
     4201 gtttttgttt accgttcata ttcaataaac gaatagaatg gatagtcgtg ttaccgacat
     4261 ctgtgacgaa aatcgcatcg tcttcagcaa tacgattgat ttctttatag acagcctctg
     4321 gacgaatcgg ttcttcctct cgatcttcaa aagatttcaa ccaatttacc catttttctt
     4381 tattttcttg atttgctagg taccagctat cagcagggcg agcatcccct aactcgacaa
     4441 gtctttgcaa agctgtgttt gcatcaccga ggatcgacaa agatgtttca tggcgtcgtc
     4501 caaattttga cgcatcgata tcgatttgga taaattcagc agttggatta aaaaatgcac
     4561 gtccaaatgg gaagtcgcta ccgacaaata agatcagatc ggcttctgcc aatgcttcat
     4621 ttccgggttt agtagcgaca cgtccagcaa aaccaagaaa gttttcataa tcatcaggaa
     4681 taattccttt tgctagaacg gaagcgacaa caggaactga aaaatgttct gagaattctt
     4741 tgatttgcgg aaatccgttt cgcgttcctt gaccaacata aagaactggt ttttttgctt
     4801 tttcaagata aggcaaagca gctagcaaat ctttttcttc tggtaatatc acacttgttt
     4861 tgtgagtgtg agcggtggag aaaggttgtt cgtcaatttc ttcaaaacca agatcaacag
     4921 ggatagtcac gatagccacg cctttatgct cataggcggc tttaatggct tcatctacta
     4981 catgcggaag actttgtggt gtcatgactg tacgattgta tacgctaaca tctgcgaaaa
     5041 taggattttc atttagttcc tggaaagagt tatagttcat ggatgtagaa gcaacttgtc
     5101 ctaagaacgc tactagaggc acatggtcca tctgtgcatc atacaaacca ttgatcaaat
     5161 gtgtcgcacc aggtcctgca gagccgaaga cagcgccgac tttccccgtt aattttgcat
     5221 ctgcagcggc tgcgagtgcg ccgacttctt catgacgaac ttgaatgtag ttgatttcgg
     5281 ttttttcttg atatagcgca tccataattg aattgaaaga tcctccggga atgccgtaaa
     5341 tatggtcgat accccagctt tcaagaactt ttaccattgc gattcctgca ttgatttttg
     5401 acatggtgtt tcactccttt agcaatgatg atacataaat tttttgtatc tagagtatag
     5461 aactttttat cgaaaatccc aactaattcg tcttttcaca aaaatgtagt catgtttttg
     5521 ggaatatgct agagtagtac atagatagat tcaagagagg ggaatggcat ggttgaatgg
     5581 ctattgagac ttgaaccttg gcagcaagca cttgctggga cagcttttac ttattttatg
     5641 acagctcttg gtgccgggtt ggtttttttc tttaaagaaa tcaaaaaaga agtgctaaac
     5701 ttgatgttag gatttgcttc tggtgtcatg attgctgcaa gtttttggtc tttgttagat
     5761 ccggctatta caaaagcaga agaaaatgga gatattgctt ggctagttgt aagtattggg
     5821 tttggtttag gcggtgtgtt tttatatatg gcagataaaa cactgcccca tatgcatttt
     5881 ggaccacaac atgaagcaga aggattgcct acccatttaa aacggacgat cttgcttgtt
     5941 ttttctatta cattgcataa cattcctgaa ggtttagccg tgggggtagc attcggtgca
     6001 gcagcaactg cggataatcc gacagccgct atattagccg ctgtttcggt ggcgctaggt
     6061 attggtatcc aaaacttccc agaaggagca gctgtatcaa tcccgttgcg acaagaaggt
     6121 ctgagtcgta aaaaggcctt tgtttacgga caagcttctg gaatcgttga accaattgct
     6181 ggagtgattg gagcgctact agtaacgaaa gtggagctgc tgcttcctta tgcacttgct
     6241 tttgccgcag gagcaatgat ttatgtcgtg gtggaagaat tgatccctga agcacagcag
     6301 acattgtcca gcaaacgtca ttttgcagtt tttggcgtca tgagtggatt tatcattatg
     6361 atgattttgg atgtggcatt aggataaata aaaaaaatat atatgatata tcaaataggg
     6421 ccgcgaactt atatcgcgac cctatttttt tagtaagtta tatcataatg aagtgaatgg
     6481 tatcatacat taataatagt tttttctatg tgtatttcct ttgatccagc gaacaagcca
     6541 gatgataagt gcgattggga ttagaacttt gaccgcaaac cataaaagcc cgcttaggac
     6601 actgacaaaa agactgatga cgaagctagc tgcaattagc actaaaagaa ccatgaagat
     6661 atttaagaac atattttttt gacgcataca aaaccctcct cttgttcatt ctttactata
     6721 gaaaaagtat aacagggatt gtttaaaaac acctccaact gaagactgat ttttacttca
     6781 tctacttaaa tgatcaaaca attgcttttt ctctggttta tcatctaaaa tagagcggaa
     6841 aaggaggaga atatcatgcc taaaaatcgt cttgaagcat ttacagatgc tgtaatcgcg
     6901 attatcatga ctattttagt tttagaactt catgaaccta acggagacac gtgggaggcg
     6961 ctcgctgcat taggttaccg attttttatc tatattgtca gttttatcat tttagcgatt
     7021 tattggaaca atcatcatca tttatttcaa gttgtacata agataaatgg acgggtctta
     7081 tgggcgaaca gtttctttgt ttttgcgctt tcgctttttc cgtttgcaac ttcatggatc
     7141 agtgagtaca tcaatagcct ggctccagaa atgacttatg gttttgttac cttgaccgct
     7201 aatattgcgt attatatctt ggcaaaagaa cttgtcaaag caaataaagg ccacgagaaa
     7261 gcattgaagg cgatgttgaa taattacaag aaatcgtatc tatcgattgg tttgaatgtg
     7321 ttaggaattg tcttagggta tttagtagcg ccagtagccg tgctgatcgc aaacatcgtg
     7381 attctagctg cttggatcat cccaagtcgt accatcgaat cacaatatcg gtaaaatgat
     7441 ccgtgtttaa aatttaagag aaaaatatag aaaaaataaa taaacgatca aatatccgag
     7501 tgatactagt attcaaagag ggatgcagat ggatcaaaaa agaattcatg taggactgat
     7561 tactagtttt gtcacgatgc ttatttattt ggatcaaggt catttgactt ggacagtagt
     7621 tggtgtcgta ggatttttga cattattcac agcaataaaa aaataaacat ctttatgtaa
     7681 tctgatgttc tatctattta agaaaagcat gtttacagca gtatgattct gttgtaaacg
     7741 tgtttttatt tgtggaaaat ttaattttta gaaaagtgta tcgaaaaata gatagttagt
     7801 ttttccacag tggataactt cgtaagagat aaaaataaaa aattagtcaa agaagggcgt
     7861 atagatttct tttgatagga gatatacaac agaaatgaat aaatattaac gtgttttcaa
     7921 acagtataaa acggtgtggt ttttggaata aaaaaataac atatctcatt ttacactttt
     7981 gttattttat acttaaaaaa taacgacaca gaaaataccg tgtctcagat ttctctacta
     8041 cttctaaaaa ctactgtttt tttcctttga aatgccttca aaaagtagga tcaggcagaa
     8101 tatactggca gaatcaagga gaagggagcc taaataagga gaaaaaccat agaaaatata
     8161 aagtaattac ttataaaaca agctttttat agaaaaataa tatacctgag tagggtataa
     8221 attaagacgg aattcctttc tagattgaga tattgactag gaagaatgtt aagattttaa
     8281 gcctttaagc taaaaaaaaa ccctttttgt taaataaggg gaatatagcc tgtcagtttc
     8341 aaactgcaag tcgtcctgct gcaataatat agtagattag agatgaaaat agattttttt
     8401 aaaccaatga ctgaataaaa agaccgtcat agatacacat tggaatgtgt ctattacggt
     8461 ctttttttac gaacgatttt attgaagacg gacagttcgg gtcaaaaata aaaaagctac
     8521 gatcagaaat aagaataaaa tgtatcccaa aataatggct agacggtcta acttatttgt
     8581 cgatagatgt ttgtttagaa gttgataggc ccataaacct atcagtcctc ctgttgtatt
     8641 agttatcaaa tcagtgatgt ccgctacgcc aataccgaat aggaattgaa tcaattctgc
     8701 caagaagctt acaaggaaaa taatccctaa ttttttagaa aaagaaagtc tcttgaaatt
     8761 gacattcaaa agcaccccaa aaggaaaaaa gacaatcaag ttataagtgg tctcacgtaa
     8821 taccgtcgta ttttcaaaag aaaaagtaga aaaagggacg agattgacat tgctgtaaga
     8881 ataagtgaga aattgtggaa gattaaatga tagcttgaat aaaatcaacc ataatagaaa
     8941 gaataaataa atccctaata aaaattttga tacataccgc atagacatct cctccattta
     9001 aaaagtatac catcgaaaac cgctgaaaaa tcataaaaaa gtatgaagtt taaggaccaa
     9061 cgtagttatc gtacgaaaat tagaagcttt ttttgttggg aatccggttt ctttaacaaa
     9121 gctaagatag cgagttgctt ggaaaagttg ctatcttttt tctgtcaaaa cgactcggaa
     9181 tgttcgtttt ttgttctaat ttctccaagt tgtttacttt ttgatactaa gtttatgaaa
     9241 aatcaagaaa tagttgttat actaggtaat gctgacaaac gggattggca tttcatcttg
     9301 gttcagcttt ttttccaatt actaggaagg agggatagat tgttcaagac ttattttcgt
     9361 ttcaataaaa tacaagataa tccttttcgt atcatgaatc tgatgctact tatctttatt
     9421 gtattatggt taggttacat aaatcatgat tttttgattt caagtttttc atcattaggg
     9481 atctttactt ttttctatta tcaaaacata ccgatgaaat atttgctgaa acgtatgttt
     9541 ctgatcggtt gcggactgtt ggttgctttc gttttgggga tcttatcgac ttatgtttta
     9601 tggcttgaac catttgccgt agctttggtt gctttttcca gcagattcgt gttgcgatta
     9661 ttccatattt ctaaaccagg tggcttgttt tttgcgatgc tttctgccat gggaacgagc
     9721 atgcagctgc caatcgctca attaccgatc gtctcacttt atttttttat gggtgtcgtt
     9781 tttgccctga ttgcggcagt gattacaaaa ttactggatt cacggcctga gcagacaatt
     9841 gaaaaagcaa ctttaaaaga aagatttcac gaagagcctt tagtgatcat tgattctgtt
     9901 ttctacagcg ctgccttatt tctttccgtt tatgtcagtc acggcttgaa tctgcataat
     9961 ccttattggt tgaccttgtc atgtgcatcg attttgctag cagaaaactt ggatgcgatg
    10021 aagcatcgac aagtccaata tctgattgga tcgatgggtg ggttatgtgt gtcggcattc
    10081 ttgtcatttg tgccgtttac gcagttgcag accatttttc tgatcacttt tctatatgga
    10141 atcgcacaat ttctagttgc tagaaattat gcagttgcta atatttttct taatccgatg
    10201 gcgttgatgc tatctacact tattcgtgga gcatacttga tttctttgat cgaatatcgt
    10261 tttttaggga tcgtcatagg aagtttcatc ggtttaggtg ttgcgtgggt aatgacggtt
    10321 ggtttgcaac attatttaac ggttgttcgt caaaaattag aatagaagga caaatatatg
    10381 tgccaaaaga ggttatgaca agatttttgt cacaacctct aattcaaggt gttcaaaagc
    10441 cagattctca gcattaagtc aaattcacac aaaaatgtgg aaaggaactt tcaaaaaaat
    10501 tttatattta ttctatcaaa aaattttgtt tatgatggta actgaataaa ctttcctgag
    10561 atggtcaatg ctttccttga aagtcttaca gatattcttt cttcatagaa agtttaagtt
    10621 ttatagttag tgtacaataa aagtatgttt ataccatatg gtgtccggat aatcagtcat
    10681 ttaaacaatg aggttcaaag atatgaaaag gggaaatttg atgaagaaaa taagcaatga
    10741 aatccaaatt ctggcagaaa ctttaactct tcccaaagtc gttatagagc aaatgattgt
    10801 acaagcaaaa aacatccttg atgaggtgtt tgagcaatat agtcatgaac taatggatcc
    10861 taataaagca aaggcgactg caaagaaaat agacaaagtc tatgaagagg aaccttttgc
    10921 agcattagct atttatctat atgcagcttc tcaaagttgg aaaaaaattt atcaaccatt
    10981 gaagattcca agaactatct atttggcaac aatgaatgct tttactagat ttattcatga
    11041 acatttccaa gtaacaggaa gctatcgctt tgaccgtggt ttttggattt ggcattacac
    11101 tagtggcttg atctttcgga ttggtgaatt ggagtacgaa atgacatatt ttccgaaagg
    11161 gtacacggat ctaagattag aaggaaagca gtgcctttct cttcatattc cttccgatgc
    11221 agatttatct cagaataaga taagagatag ttacgaaagt gccattcgtt tttttgaact
    11281 ttattttcct gattaccatt accaagtgat gtacacagat acttggttac tatcacccaa
    11341 tcttactaaa tggttgaaaa aagagtcaaa aatctgtctt tttgcagccg attatcgatt
    11401 attatcagtt gatgaacagg acgatagtgg agtcccttgg atctttggac gagtagatgc
    11461 ccaaatacat gattatccag aaagtacgag tttacagcga caagcaaaag agcaattgct
    11521 tgcaggcgag cacattggct ctggcttagg tattcttact tcataagttt agtacttcga
    11581 aatagtt
//
LOCUS       DACEUZ010000087        11346 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.087, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000087 DACEUZ010000000
VERSION     DACEUZ010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11346
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.087"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            <1..936
                     /locus_tag="IXG44_001917"
     CDS             <1..936
                     /locus_tag="IXG44_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ1658630.1"
                     /translation="GRWKETLPEISHEINIVNLTSDEKNKGLLDPYVIMKNTKDSESL
                     AIDILTFLTGISSRDGERFPILRKAIRAVTNSEVRGLMKVIEELRVENTPLSTSIADH
                     IESFTDYDFAHLLFSDGYVEQSISLEKQLNIIQVADLVLPDKETSFEEYTTMELLSVA
                     MLIVISTFALDFIHTDRSIFKIVDLDEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFV
                     TQNTDDLLDEKLKNNLGLKFAFRSTDLNEIKKTLAFFGVDPEDENNQKRLRDLENGQC
                     LISDLYGRVGVIQFHPVFEELLHAFDTRPPVRKEV"
     gene            939..3122
                     /locus_tag="IXG44_001918"
     CDS             939..3122
                     /locus_tag="IXG44_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAQ1658631.1"
                     /translation="MKPSILNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVTPSGLSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHALMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNMTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRM
                     MRKPRMLMHAHMHRLQRKLGRSVTALGAGSAIASATGKKGQSGSGSSASTQADHSRPN
                     GQEKSTLGKRIGQTIGTVADTKDRMVDTAGNLKEQVKDLPTNARYAVYQGKSKVTDNV
                     HDLTRSISQTKADKASGRKKQQEQRRKTIAERRSEMEQVKQKKQPASSVHERPATKQE
                     QSHDGQTSRQTTVQASYRESQQAKQERPTVKSDSSSLKTERQSNTVQERTVQKPVTST
                     APTDRASQRSITKERQSTVQRVPLQNTKSRPPIKTATIKKGSKKP"
     gene            3119..4120
                     /locus_tag="IXG44_001919"
     CDS             3119..4120
                     /locus_tag="IXG44_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAQ1658632.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDGGFSNIHYGGV
                     DVSAEVLAHKPMVEKVAKEYGIEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLGVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGDEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKFEGWKYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTRHIPLLEAKA
                     GDLVFFHSTYNAGSYITHIGIYLGDNRMFHAGDPIGYADLTSSYWQQHLVGAGRIKQ"
     gene            4135..5049
                     /locus_tag="IXG44_001920"
     CDS             4135..5049
                     /locus_tag="IXG44_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ1658633.1"
                     /translation="MMKFGKNQNTEKQTPKEKKPRVYKVNPRKKVVIVLWVLLGLSFS
                     FAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWELNDKSIDNRIEN
                     LKAYLTEELQALNVDTVRKDIPVSSSVKGFQIWTVEADGDNQFDVTYSVDQLITEGEN
                     TKTVHSAYIVSVYVDSTGNMVLIKNPTITSIPKKSDYKPKAIESEGTVDSITTNEINE
                     FLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIYNRKDNQVTVSLTVEY
                     IDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            5294..5410
                     /locus_tag="IXG44_001921"
     CDS             5294..5410
                     /locus_tag="IXG44_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HAQ1658634.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            5426..7345
                     /gene="tet(M)"
                     /locus_tag="IXG44_001922"
     CDS             5426..7345
                     /gene="tet(M)"
                     /locus_tag="IXG44_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HAQ1658635.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            7443..8816
                     /gene="tet(L)"
                     /locus_tag="IXG44_001923"
     CDS             7443..8816
                     /gene="tet(L)"
                     /locus_tag="IXG44_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HAQ1658636.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS
                     FLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVY
                     KHSQRDF"
     gene            9380..10642
                     /locus_tag="IXG44_001924"
     CDS             9380..10642
                     /locus_tag="IXG44_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plasmid recombination enzyme type 2"
                     /protein_id="HAQ1658637.1"
                     /translation="MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENY
                     DLKNDKNIDYNERVKEIIESQKTGTRKTRKDAVLVNELLVTSDRDFFEQLDPGEQKRF
                     FEESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPMRDGKLQGKNVFNRQELLWLQ
                     DKFPEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVKKDEWTAYS
                     DKVKSDLEVPAKRHMKSVEVPTGEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTA
                     ARDNDRLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYSGLVERFNENVNDYNELLEEN
                     KSLKSKISDLKRDVSLIYESTKEFLKERTDGLKAFKNVFKGFVDKVKDKTAQFQEKHD
                     LEPKKNEFELTHNREVKKERSRDQGMSL"
     gene            10887..>11346
                     /locus_tag="IXG44_001925"
     CDS             10887..>11346
                     /locus_tag="IXG44_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001611915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HAQ1658638.1"
                     /translation="MCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDYFELLHI
                     LEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGIQSQKV
                     VAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQGFRRMMQYKKINK"
BASE COUNT         3740 a         1897 c         2376 g         3333 t
ORIGIN      
        1 ggcagatgga aagaaacctt gccagagatt tcccatgaaa tcaatatcgt caatctgact
       61 tctgatgaga aaaacaaagg cttacttgac ccttatgtaa ttatgaaaaa taccaaagat
      121 tctgaatcac tggctattga tattttgaca ttccttacgg ggatttcctc tcgtgatggg
      181 gaacgcttcc caatccttag aaaagccatt cgtgcagtaa ccaatagtga agtgcgaggg
      241 ttgatgaaag tgattgaaga attacgggtt gagaatacgc cactaagtac cagcatagcc
      301 gaccatatcg aaagttttac agactatgac tttgcccatc tgctttttag tgatggttat
      361 gtagagcagt ctatcagcct tgaaaaacaa ctgaacatta tacaggttgc cgacttggta
      421 cttcctgaca aggaaacttc ctttgaggaa tataccacaa tggagttact atcggtagca
      481 atgctgattg tcattagtac ctttgcgttg gactttatcc atacagaccg aagtattttc
      541 aagattgtag acttagacga agcatggagc ttcttacagg tggcacaagg gaaaacacta
      601 tctatgaagc tggttcgtgc tggtcgtgct atgaacgctg gggtatattt cgtgacccaa
      661 aatacagatg acctcttaga tgaaaaactg aaaaataacc tcggcttaaa atttgccttt
      721 cgttccactg accttaacga gattaaaaaa acccttgcct tttttggtgt agacccagag
      781 gacgaaaaca atcagaagcg attgcgtgat ttggaaaacg ggcaatgcct tatcagtgat
      841 ttatatggtc gtgtcggtgt gatacagttc caccctgtat ttgaagaact gctccatgcc
      901 tttgatacca gaccacctgt gcgaaaagag gtgtaaatgt gaaaccatca atattaaaca
      961 gaataaaatc aaactggacg ctgaaacgtc taggtaaagt ggcaatgaca gtggctttca
     1021 cacttgtgat tgccattttt cttttagcca tgctgggaac ggtggttcaa gctgcgggct
     1081 tggtagatga tacggtcaat gtggcaaatg aatacagccg atacccactt gaaaactatc
     1141 aactggattt ttatgtggat aatagctggg gctggctacc gtggaactgg tcggacggaa
     1201 ttggaaaaca agtcatgtat ggactatatg ccattaccaa ttttatttgg acaatcagtc
     1261 tttatgtttc caatgcgacg ggttacttag tacaggaagc ctattcctta gacttcattt
     1321 ccgctacagc agattccatt ggcaagaata tgcagaccct tgcaggggtt acgcctagtg
     1381 gactatcaac agagggtttc tatgttggat tccttttact cttgattttg gttcttgggg
     1441 tttatgttgc ctatacagga ctgataaaga gagaaaccac aaaggcaatt catgccctca
     1501 tgaattttgt gctggttttt atcctatcgg cttcctttat tgcctacgct cccgactaca
     1561 ttaaaaaaat caatgacttt tcatcagaca tcagcaacgc tagtttgtca ctgggtacaa
     1621 aaattgtcat gccccattca gatagtcaag gcaaggacag tgtggacttg atacgtgaca
     1681 gcctattttc catacaggtt cagcaaccgt ggctactgct tcaatataac agttcagaca
     1741 ttgaaagtat tggtattgac cgtgtggaaa gcctgctctc aaccagtcca gattccaaca
     1801 atggggaaga cagagaaaaa attgttgcgg aagaaattga agatagaagc aataccaata
     1861 tgacgattac aaagacgatt aaccgtttag gtacagtctt cttcctattt gtcttcaata
     1921 ttgggatttc catatttgta ttcctattaa caggaatcat gattttctcg caggtacttt
     1981 ttatcatcta tgctatgttt ctgcctgtga gctttatttt aagcatgatt ccatcatttg
     2041 atggtatgtc aaaacgagcc ataacaaagc tctttaatac cattttgaca cgagctggaa
     2101 tcacattgat tattacgaca gcatttagta tttcaaccat gctctatacc ttatcggctg
     2161 gttatccgtt ctttttgatt gcttttctac agattgtgac ctttgcagga atctacttta
     2221 agctgggcga tttaatgagt atgttttctc tacagagtaa cgattctcaa agtgtgggaa
     2281 gtcgtatgat gagaaaacct cgtatgctta tgcacgctca catgcaccgt ctacagcgga
     2341 aacttggacg ctccgtgact gctttagggg ctgggtctgc cattgccagt gctacgggta
     2401 aaaaaggaca gtcgggttcg ggaagttctg cgagtacaca agcagatcac tctcgaccaa
     2461 acgggcagga aaaatcaaca ctaggaaagc gtatcggtca aaccatcggt acagtagctg
     2521 ataccaaaga cagaatggta gatactgctg gtaatttgaa agaacaggtt aaggatttgc
     2581 cgaccaatgc aagatatgca gtatatcaag gaaaatctaa agtgacggat aatgtccatg
     2641 atttaaccag aagtatttct caaaccaaag cggataaagc cagtggacgc aagaaacagc
     2701 aggaacaaag acggaaaacc attgcggagc gtcgctctga aatggaacag gtcaaacaga
     2761 aaaaacagcc agcttcttcc gttcatgaaa gaccagccac taagcaagaa caatctcatg
     2821 atggacagac ttcaaggcaa acaactgtac aggcttccta tagggaatct caacaagcca
     2881 aacaagagcg tccaacagtt aagtccgatt cttcaagttt aaaaacggaa cgtcaaagta
     2941 atacagttca agaaagaacg gttcaaaagc cagtaacttc aactgcacca acagatagag
     3001 cttcacaacg ttcaatcaca aaagaacgtc agtctactgt tcaaagagta ccactacaaa
     3061 atacaaaaag tagaccacca atcaaaaccg ccaccattaa gaaaggcagt aagaaaccat
     3121 gaagctgaaa actttagtga ttggtggttc gggattattc ttgatggtct tctcactgct
     3181 tctgtttgtc gccattttat tttcagatga acaagatggc ggtttttcca acattcacta
     3241 tggcggtgtg gatgtttccg cagaagtgct ggctcataag cctatggtag aaaaagttgc
     3301 caaagaatat ggaattgaag aatacgtcaa tatacttctt gcgattatac aggtggaatc
     3361 gggtggtaca gcagaagatg ttatgcagtc ctcggaatcc ctcggtcttc cacctaattc
     3421 attgagtaca gaagaatcca ttaagcaagg tgtgaagtat ttcagtgagt tattagccag
     3481 tagcgaaagg cttggtgtag atttagaatc ggttatccag tcctacaatt atggtggtgg
     3541 tttcttagga tatgtggcta atcgtgggaa taaatatacc tttgaactgg ctcaaagttt
     3601 ctcaaaagag tattcgggtg acgaaaaagt gtcttacccc aatcccatag ccatacctat
     3661 caatgggggc tggcgataca attacgggaa tatgttttat gtgcaactgg taacgcagta
     3721 tcttgtcaca acagagtttg atgatgatac agtacaagcc atcatggacg aagctctgaa
     3781 atttgaaggc tggaagtatg tctatggcgg agcttccccg actacctctt ttgattgtag
     3841 cggactgaca caatggacgt atggaaaagc tggaatcaac ttaccacgaa cagcacaaca
     3901 gcaatatgat gtaacccggc atatcccatt attggaagca aaagccggcg atttggtttt
     3961 ctttcattct acctataacg ctggctctta tattactcat atcgggattt atttaggcga
     4021 taaccgtatg tttcatgcag gcgaccctat cggttatgcc gacttaacaa gctcttactg
     4081 gcaacagcat ttagtgggag caggacgaat caaacaataa gaaaggacga ttcaatgatg
     4141 aaattcggaa aaaatcagaa tacagaaaaa cagacaccaa aggaaaagaa acctcgtgtc
     4201 tataaggtca atcctcgtaa aaaggttgtg attgtcctat gggtactttt aggacttagt
     4261 ttcagctttg cgatattcaa gcactttaca gccattgata cccataccat tcatgaaacc
     4321 accatcatag aaaaggaata tgtcgatact catcatgtgg aaaattttgt agagaacttt
     4381 gcgaaagtct actattcatg ggaactaaac gacaagtcca ttgataatcg catagaaaac
     4441 ctgaaagcat atctgacaga ggaacttcaa gctctgaatg tcgatacagt tcgtaaagat
     4501 attcctgtat cgtcttctgt aaaaggattt cagatatgga cggtagaagc agatggcgac
     4561 aatcaatttg atgtaaccta cagtgtagac caactcatta cagaggggga aaatacaaag
     4621 accgtccact ctgcttatat agtgagtgtc tatgtagaca gtactggaaa tatggtcctc
     4681 attaagaatc cgaccattac tagcatacca aagaaatcag actataaacc aaaagctatt
     4741 gaaagtgagg gtacggttga ttccattaca accaatgaaa tcaatgagtt tttaacgacg
     4801 ttcttcaagc tctatcctac agcgacagcc agtgaacttt cctactatgt gaatgacggg
     4861 atattaaaac caatcggaaa agagtacatc tttcaagaac tggtaaatcc tatttacaat
     4921 cgtaaggata atcaagtcac ggtatcgctg acagtggagt atatcgacca gcagaccaaa
     4981 gcaacgcagg tatctcaatt tgatttggta cttgaaaaga acgggagtaa ttggaagatt
     5041 atagaataac aaatattggt acattattac agctattttg taatcacgta ctctctttga
     5101 taaaaaattg gagattcctt tacaaatatg ctcttacgtg ctattattta agtatctatt
     5161 taaaaggagt taataaatat gcggcaaggt attcttaaat aaactgtcaa tttgatagtg
     5221 ggaacaaata attggatgtc cttttttagg agggcttagt tttttgcacc cagtttaaga
     5281 atacctttat catgtgattc taaagtatcc ggagaatatc tgtatgcttt gtatgcctat
     5341 ggttatgcat aaaaatccca gtgataagag tatttatcac tgggattttt atgccctttt
     5401 gggcttttga atggaggaaa atcacatgaa aattattaat attggagttt tagctcatgt
     5461 tgatgcggga aaaactacct taacagaaag cttattatat aacagtggag cgattacaga
     5521 attaggaagc gtggacaaag gtacaacgag gacggataat acgcttttag aacgtcagag
     5581 aggaattaca attcagacag gaataacctc ttttcagtgg gaaaatacga aggtgaacat
     5641 catagacacg ccaggacata tggatttctt agcagaagta tatcgttcat tatcagtttt
     5701 agatggggca attctactga tttctgcaaa agatggcgta caagcacaaa ctcgtatatt
     5761 atttcatgca cttaggaaaa tggggattcc cacaatcttt tttatcaata agattgacca
     5821 aaatggaatt gatttatcaa cggtttatca ggatattaaa gagaaacttt ctgccgaaat
     5881 tgtaatcaaa cagaaggtag aactgtatcc taatatgtgt gtgacgaact ttaccgaatc
     5941 tgaacaatgg gatacggtaa tagagggaaa cgatgacctt ttagagaaat atatgtccgg
     6001 taaatcatta gaagcattgg aactcgaaca agaggaaagc ataagatttc agaattgctc
     6061 cttgtaccct gtttatcatg gaagcgcaaa aagcaacata gggattgagc agcttataga
     6121 agtgataacg aataaatttt attcatcaac atacagaaag aagtctgaac tttgcggaaa
     6181 tgtcttcaaa attgaatatt cggaagaaag acaacgtctt gcatatgtac gcctttatgg
     6241 cggaatcctg catttgcggg attcggttag aatatcggaa aaggaaaaaa taaaaattac
     6301 agaaatgtat acttcaataa atggtgaatt atgtaaaatt gataaggctt attccgggga
     6361 aattgttatt ttgcaaaatg agtttttgaa gctaaatagt gttcttggag atacaaagct
     6421 attgccacag agagagagaa ttgaaaatcc gctccctctg ctgcaaacaa ctgttgaacc
     6481 gagcaaacct caacaaaggg aaatgttact tgatgcactt ttagaaatct ccgacagtga
     6541 cccgcttcta caatattatg tggattctac gacacatgaa attatacttt ctttcttagg
     6601 gaaagtacaa atggaagtga ttagtgcact gttgcaagaa aagtatcatg tggagataga
     6661 actaaaagag cctacagtca tttatatgga gagaccgtta aaaaatgcag aatataccat
     6721 tcacatcgaa gtgccgccaa atcctttctg ggcttccatt ggtttatctg tatcaccgct
     6781 tccgttggga agtggaatgc agtatgagag ctcggtttct cttggatact taaatcaatc
     6841 atttcaaaat gcagttatgg aagggatacg ctatggttgc gaacaaggat tatatggttg
     6901 gaatgtgacg gattgtaaaa tctgttttaa gtatggctta tactatagcc ctgttagtac
     6961 cccagcagat tttcggatgc ttgctcctat tgtattggaa caagtcttaa aaaaagctgg
     7021 aacagaattg ttagagccat atcttagttt taaaatttat gcgccacagg aatatctttc
     7081 acgagcatac aacgatgctc ctaaatattg tgcgaacatc gtagacactc aattgaaaaa
     7141 taatgaggtc attcttagtg gagaaatccc tgctcggtgt attcaagaat atcgtagtga
     7201 tttaactttc tttacaaatg gacgtagtgt ttgtttaaca gagttaaaag ggtaccatgt
     7261 tactaccggt gaacctgttt gccagccccg tcgtccaaat agtcggatag ataaagtacg
     7321 atatatgttc aataaaataa cttagtgtat tttatgttgt tatataaata tggtttcttg
     7381 ttaaataaga tgaaatattt tttaataaag atttgaatta aagtgtaaag gaggaattaa
     7441 ttatgaagtg taatgaatgt aacagggtta aatacaacca aattttaatt tggctttgcg
     7501 ttttatcttt ttttagcgta ttaaatgaaa tggttttgaa cgtctcatta cctgatattg
     7561 caaatgattt taataaacca cctgcgagta caaactgggt gaacacagcc tttatgttaa
     7621 ccttttccat tggaacagct gtatatggaa agctatctga tcaattaggc atcaaaaggt
     7681 tactcctatt tggaattata ataaattgtt tcgggtcggt aattgggttt gttggccatt
     7741 ctttcttttc cttacttatt atggctcgtt ttattcaagg ggctggtgca gctgcatttc
     7801 cagcactcgt aatggttgta gttgcgcgct atattccaaa ggaaaatagg ggtaaagcat
     7861 ttggtcttat tggatcgata gtagccatgg gagaaggagt cggtccagcg attggtggaa
     7921 tgatagccca ttatattcat tggtcctatc ttctactcat tcctatgata acaattatca
     7981 ctgttccgtt tcttatgaaa ttattaaaga aagaagtaag gataaaaggt cattttgata
     8041 tcaaaggaat tatactaatg tctgtaggca ttgtattttt tatgttgttt acaacatcat
     8101 atagcatttc ttttcttatc gttagcgtgc tgtcattcct gatatttgta aaacatatca
     8161 ggaaagtaac agatcctttt gttgatcccg gattagggaa aaatatacct tttatgattg
     8221 gagttctttg tgggggaatt atatttggaa cagtagcagg gtttgtctct atggttcctt
     8281 atatgatgaa agatgttcac cagctaagta ctgccgaaat cggaagtgta attattttcc
     8341 ctggaacaat gagtgtcatt attttcggct acattggtgg gatacttgtt gatagaagag
     8401 gtcctttata cgtgttaaac atcggagtta catttctttc tgttagcttt ttaactgctt
     8461 cctttctttt agaaacaaca tcatggttca tgacaattat aatcgtattt gttttaggtg
     8521 ggctttcgtt caccaaaaca gttatatcaa caattgtttc aagtagcttg aaacagcagg
     8581 aagctggtgc tggaatgagt ttgcttaact ttaccagctt tttatcagag ggaacaggta
     8641 ttgcaattgt aggtggttta ttatccatac ccttacttga tcaaaggttg ttacctatgg
     8701 aagttgatca gtcaacttat ctgtatagta atttgttatt acttttttca ggaatcattg
     8761 tcattagttg gctggttacc ttgaatgtat ataaacattc tcaaagggat ttctaaatcg
     8821 ttaagggatc aactttggga gagagttcaa aattgatcct ttttttataa caggaattca
     8881 aatctttttg ttccattaaa gggcgcgatt gctgaataaa agatacgaga gacctctctt
     8941 gtatcttttt tattttgagt ggttttgtcc gttacactag aaaaccgaaa gacaataaaa
     9001 attttattct tgctgagtct ggctttcggt aagctagaca aaacggacaa aataaaaatt
     9061 ggcaagggtt taaaggtgga gattttttga gtgatcttct caaaaaatac tacctgtccc
     9121 ttgctgattt ttaaacgagc acgagagcaa aacccccctt tgctgaggtg gcagagggca
     9181 ggtttttttg tttctttttt ctcgtaaaaa aaagaaaggt cttaaaggtt ttatggtttt
     9241 ggtcggcact gccgacagcc tcgcagagca cacactttat gaatataaag tatagtgtgt
     9301 tatactttac ttggaagtgg ttgccggaaa gagcgaaaat gcctcacatt tgtgccacct
     9361 aaaaaggagc gatttacata tgagttatgc agtttgtaga atgcaaaaag tgaaatcagc
     9421 tggactaaaa ggcatgcaat ttcataatca aagagagcga aaaagtagaa cgaatgatga
     9481 tattgaccat gagcgaacac gtgaaaatta tgatttgaaa aatgataaaa atattgatta
     9541 caacgaacgt gtcaaagaaa ttattgaatc acaaaaaaca ggtacaagaa aaacgaggaa
     9601 agatgctgtt cttgtaaatg agttgctagt aacatctgac cgagattttt ttgagcaact
     9661 ggatccagga gaacaaaaac gattttttga ggaaagttat aaattatttt ccgaacgata
     9721 tggcaagcaa aatattgctt atgcaacagt tcataatgat gagcaaaccc ctcacatgca
     9781 tttaggtgtt gtgcctatgc gtgatggaaa actgcaagga aaaaatgtgt ttaatcgtca
     9841 agaactgtta tggctacaag ataaattccc cgagcatatg aaaaaacagg gttttgagtt
     9901 gaagcgtggt gaacgtggct ctgaccgtaa acatattgag acagctaaat ttaaaaaaca
     9961 aactttggaa aaagagattg attttctaga aaaaaattta gcagttaaaa aagatgaatg
    10021 gactgcttat agcgataaag ttaaatcaga tttagaagta ccagcgaaac gacacatgaa
    10081 aagtgttgaa gtgccaacgg gtgaaaagtc catgtttggt ttgggaaaag aaataatgaa
    10141 aacagaaaag aaaccaacca aaaatgttgt tatatcggag cgtgattata aaaacttagt
    10201 gactgctgcg agagataacg ataggttaaa acagcatgtt agaaatctca tgagtactga
    10261 tatggcgaga gaatataaaa aattaagtaa agaacatggg caagttaaag aaaaatatag
    10321 tggtcttgta gagcgattta atgaaaatgt aaatgattat aatgagttgc ttgaagaaaa
    10381 caagtcttta aagtctaaaa taagcgattt aaagcgtgat gtgagtttaa tctatgaaag
    10441 cactaaggaa ttccttaagg aacgtacaga cggcttaaaa gcctttaaaa acgtttttaa
    10501 ggggtttgta gacaaggtaa aggataaaac agcacaattc caagaaaaac acgatttaga
    10561 acctaaaaag aacgaatttg aactaactca taaccgagag gtaaaaaaag aacgaagtcg
    10621 agatcaggga atgagtttat aaaataaaaa aagcacctga aaaggtgtct ttttttgatg
    10681 gttttgaact tgttctttct tatcttgata tatatagaaa taacgtcatt tttattttag
    10741 ttgctgaaag gtgcgttgaa gtgttggtat gtatgtgttt taaagtattg aaaaccctta
    10801 aaattggttg cacagaaaaa ccccatctgt taaagttata agtgaccaaa caaataacta
    10861 aatagatggg ggtttctttt aatattatgt gtcctaatag tagcatttat tcagatgaaa
    10921 aatcaagggt tttagtggac aagacaaaaa gtggaaaagt gagaccatgg agagaaaaga
    10981 aaatcgctaa tgttgattac tttgaacttc tgcatattct tgaatttaaa aaggctgaaa
    11041 gagtaaaaga ttgtgctgaa atattagagt ataaacaaaa tcgtgaaaca ggcgaaagaa
    11101 agttgtatcg agtgtggttt tgtaaatcca ggctttgtcc aatgtgcaac tggaggagag
    11161 caatgaaaca tggcattcag tcacaaaagg ttgttgctga agttattaaa caaaagccaa
    11221 cagttcgttg gttgtttctc acattaacag ttaaaaatgt ttatgatggc gaagaattaa
    11281 ataagagttt gtcagatatg gctcaaggat ttcgccgaat gatgcaatat aaaaaaatta
    11341 ataaaa
//
LOCUS       DACEUZ010000088        11286 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.088, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000088 DACEUZ010000000
VERSION     DACEUZ010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11286
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.088"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            267..1637
                     /locus_tag="IXG44_001926"
     CDS             267..1637
                     /locus_tag="IXG44_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1658639.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            complement(1692..2588)
                     /locus_tag="IXG44_001927"
     CDS             complement(1692..2588)
                     /locus_tag="IXG44_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ1658640.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(2869..3546)
                     /locus_tag="IXG44_001928"
     CDS             complement(2869..3546)
                     /locus_tag="IXG44_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAQ1658641.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            complement(3637..4542)
                     /locus_tag="IXG44_001929"
     CDS             complement(3637..4542)
                     /locus_tag="IXG44_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ1658642.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     KNFKNIALDTLKKQLEKQTAVSWTT"
     gene            4717..6171
                     /locus_tag="IXG44_001930"
     CDS             4717..6171
                     /locus_tag="IXG44_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1658643.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMEDELHDAVVPSH"
     gene            6187..7218
                     /locus_tag="IXG44_001931"
     CDS             6187..7218
                     /locus_tag="IXG44_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAQ1658644.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGHFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            7225..8412
                     /locus_tag="IXG44_001932"
     CDS             7225..8412
                     /locus_tag="IXG44_001932"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HAQ1658645.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            8568..9950
                     /locus_tag="IXG44_001933"
     CDS             8568..9950
                     /locus_tag="IXG44_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAQ1658646.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            9922..10308
                     /locus_tag="IXG44_001934"
     CDS             9922..10308
                     /locus_tag="IXG44_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1658647.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(10508..11176)
                     /locus_tag="IXG44_001935"
     CDS             complement(10508..11176)
                     /locus_tag="IXG44_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HAQ1658648.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
BASE COUNT         3284 a         2201 c         2207 g         3594 t
ORIGIN      
        1 tcttgaatta caatggccgt acgttccaaa tcttacactc ctttttaggt ttacctaact
       61 ttttatttaa atatagttta actaaaatgg aaagcatttt caagtagaat gatgaaaaag
      121 tttttttcga attttcctcg cattttatga aaattttctg tagaatcaca tcaagagttc
      181 aataaggcta acctaataat gaaaaacatg tgttgaaacg tttgattatt ttttcttgtt
      241 gactcaacaa cgaaaggagt ttttgtatgg aagaagaaaa gttaactcga agtcttagtg
      301 cccgtcatat ccaaatgatc gcactaggtg gaacgatcgg tgtcggtctg tttatgggtg
      361 cctcctcaac catacgttgg acagggccct cagtgatggg agcctatgcg attgcaggat
      421 tctttttgta tttgattatg cgtgctttag gcgaaatgct ttacgttgat ccttcgactg
      481 gttcttttgc aaattacgcg agcgagtaca tccatcctgt ggctggctac ttgaccgctt
      541 ggagtaatat ttttcaatat attgtcgtag gaataagtga agtgattgct gtaggatctt
      601 atatgaatta ttggtggcct gatttgccgg caattattcc cggaatcatt gtcgtcttgt
      661 ttcttatgct ggcaaatctt atttctgtca aggcgtttgg cgaattagaa ttttggtttt
      721 cgatcatcaa agtcataacg atcattttga tgatcattgc aggtcttggg gtaatcttat
      781 ttggatttgg taatcatggt caggcagtag ggatttcaaa tctttggaaa aatggtggat
      841 tcttcactgg aggcgtcaaa ggatttttct ttgcattatc gatcgttgtt gcttcctatc
      901 aaggaatcga actgatcgga atgactgctg gtgaagcaga gaatcctaaa aagacgatca
      961 ttgaagctgt ccagtccact atcggtcgaa tcttgatttt ttatattggt gctattttta
     1021 tcattgtgag tatctatccg tggaatcaat tgagtgagat tggttcacct tttgtacaaa
     1081 ctttttctaa agtcggcatt acatttgctg caggactgat taattttgtc gtgattacag
     1141 cggcattatc aggttgcaat tcagggattt tcagcgcaag tcgtatggtc tatacacttg
     1201 ccataaatgg aaagatgtct aaaaaatttc tgaaactatc tcgtcacggt gcgccttttt
     1261 atcctgtcgt agcaatctca ggtggaatct taattggtct gatcttaaat tatcttctac
     1321 caatgttcat caaccagtca gaaaatattt tcgttttcgt atacagctca agtattttgc
     1381 ctggtatggt tccttgggta gtgattttga tcagcgagat tcgcttcaaa aagaagcacg
     1441 cagataaaat gcatgatcat ccttttaaaa tgccactatc tccttatacg aattatgtga
     1501 cacttgcttt tttagctatc gtattgattt tcatgtttat caatcctgaa acgagaatct
     1561 cattgattat tggactcgtt ttcttgcttt acatgacgat ccattacttt gtacgcgaaa
     1621 aaagaaccgg aaaataaaaa aagaacgaag cttcacctta gtggaagctt cgttcttttt
     1681 tattgctttt atcaattcaa ttttagcgtc tcaccgggag tcaggaaata attctccatg
     1741 ctcatcccat tcagcgacaa gatcgtttct actggtacgc cagttcgttc ggctatttga
     1801 tttggacctt ctccttccaa aactacggtt gtcgcagcct gtgaagtcgc gctgttggta
     1861 ttagtttcag aggaatctgt tgtgtcttgt gttgttcgag cttgcatttc agaagcttct
     1921 accgactctg ctgtctcaga atcagaagat gttgtgtctt gtgtgcttgt tgactctgct
     1981 tgaagagagg cgctagtctg caaataaata tcggaaatct tagcgacatg attcgtataa
     2041 ttagccgata attgattgat caaagtatct aagtctacac cgtctttatt ttctgctgcc
     2101 tgatacgctg cacgtaattt tctgatccct tcgtcggaga ttgcttgaag ctcacgtgct
     2161 ttttggttag caactgctga taaaccagct attccctctt gatttccctg aatttctgtc
     2221 tggtattctt caatcaatgc aggcgtcttt tcttcgatct ttttcgaata atcatttacg
     2281 attgcttgaa gttgctctgg caaaccattt gctgtttttt cttcagaagt ttgtgtctct
     2341 tttgttttag ttgatgactc acttgtttgt ttcgtctctg ttttctttgt cgttgtttcc
     2401 gttatggtcg tacttttcgc ttctgacgaa ttttttccat gaaaatatag acccgcaact
     2461 cctcctgcta tgatcaaaaa cacggcaact gctgccccaa tcatgatcat ccgcttacga
     2521 gctgcctgtt tttgctgtct tcttcgggtc atccgaacat tttctcccca ctcattttca
     2581 tcatacattt ttttcaccac caatttttat gtataatagc gttatcaaac gcttcatatt
     2641 cttattgtag caaataattg tcgctgtgca catttttatc tttgttattt ataagaaaat
     2701 ttcaccagtt gagatgattc caagattgtc cgtcgatttc tccttgcagc tgcattgttc
     2761 gttcgtctat ctcagccatc agtttcttta tttcatataa gagcgcacgg tcttgatatt
     2821 ctgatttttc gcttaattca tctaattgtt ggatcagcca atcatttgtc ataatgaagt
     2881 tcctttcatt tcttgcagtg taaaggtcaa gtcttcgatc tgctcttttg cctcagtcag
     2941 tgcttctacg atatatgctt gtttttcaga ttcttcttcc tgcattttct gattttcctg
     3001 tactctttga ataaaccagt caatccgctg agtcagcgat ggtttgcctt ccaatgagag
     3061 atactgctga taagcaatta gcttctccca acgtttttcc tgggataaat acataaattg
     3121 tgctaaaacg aatggcggaa gataggtcat atggaattta tttgcgattc gctgatacgg
     3181 cctcaaaaat tcggaaatcg tatattcttc ttttcctcca gcttggtatt ctttttccgc
     3241 cacacctgtt gtcaatacga ttcctagttc tttccctttt aatacatgtg ctgctcgttc
     3301 caagacatta tcttgccaaa tttttaaatg agcaggagaa ctataccagt aaaacggaaa
     3361 ctggaaaatg atccgatcat gctgggccaa gagctgtagt tcagtctcta tatctatttt
     3421 tccgtctgga taacttgatt ccaaatgatg ccaagtagct ggtaaaccag cagaagcttc
     3481 tttgaaaaat tgctgattta aagattcttc gatcgttgga tggctaatga tgataagtgt
     3541 acgcatccct tttcctcctt tttagtcttc gctttacagt gtagcataac cgccacaagt
     3601 ttcttttgcc gatgcttgtc attttaacca gactaatcag gttgtccaag aaactgcggt
     3661 ttgtttttcc aactgttttt tcaaggtatc caaagcaata ttcttaaaat ttttgttttt
     3721 catacgttta cgatagttcg tagccgtttc tccggtactc tttttgaaca ttcggctaaa
     3781 tacgactaga ctattgaacc ctacttgata ggcaatactt tccacggatt catccgtcag
     3841 agaaagaaga aacgcccctc gattgatccg taccccgatc aaatattcct tgatactggt
     3901 acccatatga tttttgaata ctcgcgacaa atggctccga ttgaccgcta aatgctgggc
     3961 gatttcctca acagacaatt tttcagcata atgtgcctcg atgtatttga ctgtttcaac
     4021 agctaaagag gaatgaacct tttgtacacc accgctgacc tttccgccat cttttaaaag
     4081 gactgataaa aaacggtagg ttaatgctgt taactgtaat tcattttcca aggtttcttc
     4141 cgtatctttc atgcattcta aaataatgtc gatatatttt tgtatgtcag aagagaccat
     4201 cgtgtattgg tcttctctga aaagcgactg ccgaatgatt tcttctgcag cactcccacc
     4261 aaatgaaagc cagcaataca tccacggatc ttctccatct gataagtaaa acgtcgagtc
     4321 tcctggacga atcaagaaca gatcaccttt tttcaattgg aattgttgat ttttcacatg
     4381 ataagatcct ttgccttcca tcacgacatg caggatgtaa tcattgcgaa tagcaggacc
     4441 aaaagaatga aacggcatcg ttttagagaa accacaaaaa tcaaaataca attctttttc
     4501 tacttgttga tgttcctttt ttaaaaaaat cgattgctcc acgtctatcc ctccttactc
     4561 ctttagtata atgcaacaaa aagttaaata aaagtcacag aaatatatta aaaaggaagc
     4621 gatttcttgt taagtttaag tcattaaaag atctttgtta ttcgttgtaa ccggtttact
     4681 tagtattata tattgaacag gagaggaata cagattatga aaagcaaaat gatccaacgt
     4741 gtgtcctacg ctttaggcgc ttttggacac gacacattct atgttacatt aagcacttat
     4801 tttatggtat tcgtgacaag ccagatgttc gcaggaacag ataaggcaac gaatgcaaaa
     4861 atgattgcta cagttacaag cttagttgta ggtatccgct tagttgaaat tatttttgat
     4921 cctttgattg gtggcatcat cgataatact cgtacaaaat acggtaaatt caaaccatgg
     4981 ttagttatcg gcggcgttgt tagttccgta ctcttggtag caatctttac caatttcttt
     5041 ggcttagcta ctcataatac gactttattt atcatattat ttgcaatcgt tttcatctta
     5101 ttggatagtt tttattcatt taaggatatc gctttttggt caatgattcc agctttgagt
     5161 acggaaacaa aggaacgtga aaaatttgct acgtttgctc gtttcggctc ttctctaggt
     5221 gcaaatggta ctactctagt cgttgtccct atcgttactt tctttaccta tcttgttact
     5281 cataaacata ttgaaggcgc ttcaggctgg ttctggttcg gtgtgattgc tgctgttatt
     5341 tcaggcggta ctgcgattat cactgccttg ggcacaaaag aagtcgatac tgcgatccgt
     5401 cagcaaaatg aaaaagctgg gatcaaagat gtgttcaaag cagttgctca aaatgatcag
     5461 ttgatgtggc ttgcccttgc ttatatcgct tatgcgattg ccaatgcagc aacaactgcc
     5521 gtattattct acttcttcaa atatgtactt ggccaaccaa ctgaattttg gatcgttggt
     5581 ctagttgctg ccttacttgg tttagtagct gttccactat tcccaatgct tacacgcgtc
     5641 atctctcgtc gctacgttta ccttggaggg atcgcaatga tgatttcttc ttactttttc
     5701 tttacgatcg caggttcaag tttgccaatc gttatcatcg ggctagtatt gttctacttc
     5761 cctcaacaat tgattttctt atccgctttg atgacgatca cagactctgt tgaatatggt
     5821 caatggaaaa atggtatccg taacgaagct gttactctat caattcgccc tttactagat
     5881 aaaatcgctg gtgctttgtc taacgggatc gtagggtttg tcgctatcgc ttctgggatg
     5941 actggtaatg ctactgctgc tgatatcact gctcatggcg taacgacttt caaagcttat
     6001 gcattctatg ctcctgctgc tttgatgatt ttctccgcta ttatttttgc ttggaagatt
     6061 acattgacag agaaaaaaca tgcacaaatc gttgagaaat tggaaaacaa attggctcca
     6121 gctgatacaa tggaagatga acttcacgat gcagtagttc cttcccatta agaaaggatc
     6181 ttgaaaatga agattacaga aaaagatttt ggccaaggtt atcacttgat aaccttggag
     6241 aacaaaaaca agctagcact ttctatctct gatttaggtg ctagaatcgt cagtttaaaa
     6301 tcaaatgatc gagaattagt tttgggattt gacaccgctg aagaatatat cgaaaaagat
     6361 ccgtatatcg gtgcttctat cggtcgcacc gcagggagaa tcgaaaatgg ccacttttct
     6421 ctcaatggaa aaacttacca attagccaca gatccaaaaa ctggacataa tctgcatggt
     6481 ggtgctcctg gatttgaatt aaaaaaatgg tcttatgtca ttttgaatgg cgaaaatgaa
     6541 gcttctgtca tttttactac tacaagtccc gacggagaac atggttttcc aggtacaatg
     6601 gatgtggaaa tccgctatac tttgacaaaa gataatatct ggcgagtgac ctctcgcggc
     6661 accagtgacc aagatacttt attcaatccg acaaatcatg tttacttcaa cttgacggga
     6721 gatgcttctc agtcaatcga tcaacatgag ctttggctca atagcgaagc ttatgctcct
     6781 ttgcgaacag atagtattcc tataggggta aaagaaaatg ctgcaggaag tgctttcgat
     6841 tttcaaatac ctaaaaaatt agcgtccgtt tttgctagtg atttggatca aaaaaatctt
     6901 gttgacggaa tcgatcatcc atttttctta aaagaaaccg gcttgggtaa agaagccgct
     6961 cgcttgacta gccccgataa aagaatccaa gtagatattg ctacagatgc ttctagtgtg
     7021 gttattttca ctgcaaattt cggcacagaa acaccagaaa tgcgcaaccg aaaactggca
     7081 catcacggtg gcatcacatt tgagactcaa acagctccag gtgcagagcg cttctcagca
     7141 tttggttcga ttcatttaaa agccggatca gtatttgaaa cagtgacaga attcaaaatc
     7201 aaaacaagaa aggaataact catcatggaa atcaaaagaa gcttaaacac aaaatttgcc
     7261 gaaacattcg gacctaaaaa aactacacaa tacttctcac caggacgcat caatttgatt
     7321 ggtgaacaca cagattacaa tggaggccat gtcttccccg cttccatcac ttatggaacg
     7381 caaggtgtag cagcacctcg agacgataat aaaatattag tttattccac aaactttgac
     7441 gatatcggtg tcattagttt tactttagat gagctagaat atgataaaaa agcaaactgg
     7501 gcaaattacg taaaaggaat ggtccttaaa ttaaaagaag ctggacactc aatcgatcat
     7561 gggtttgagt tattagtgga aggaacaatc cctaatggtg caggactttc cagtagcgct
     7621 tcattagaat tattagtagg tgttgtatta gaagatctgt ttgatttaaa aacagatcgc
     7681 ttggaactag tccaaacagg taaaaaagta gaaaatgaat tcatcggtgt caattcaggt
     7741 atcatggatc agtttgccat cggcttcggt gagctagatc atgcaatctt attagataca
     7801 aatacgctga aatacgaaat ggttccagtg aaactagatg gttatgcaat cgtcatcatg
     7861 aatacgaata agcggcggga attggcagat tcaaaataca atgaacgccg tagcgaatgc
     7921 gaagaagcct tggcacgttt gcaaaaaact ttgcctatct cttctttagg agacttagat
     7981 gaagaagaat ttgaatcaac tattgaccaa atcggcgatg atacattgat acgcagagct
     8041 cgccatgccg tttctgaaaa tcagcggact ttgaaggcaa aagctgaatt agaagctggg
     8101 aacttagaag catttggtca actgttaaat gattctcacc actctcttcg ctatgattat
     8161 gaagtaactg ggatcgaatt agatacacta gtagatgctg ctcaaaaaca agaaggtgtc
     8221 ttaggtgcac gaatgacagg agctggattt ggtggctgtg cgattgctct tgtcaaagaa
     8281 gaaaatatac ctgccttcaa aaacaatgtc tatgatgaat acatgaaagt tgtcggctat
     8341 gctccggaat tttatgttgc acacattgga aatgggacaa caaagatcga tccagaagct
     8401 gttcatgttt gataaaaaaa tcaacgatct cagctaaaaa caaatagata aaaataacct
     8461 ctcagcaatt tccaaatgtg agccttttgc tcgtatttga aaattgctgt tttttattga
     8521 cacggaaaga gaacgtgtgt tctaattgtg ttgaagaggt gattgttatg tgggcgaatc
     8581 aaaatccggt atttgtttat gaaaaagaac cttcaagaga tattttatgt attgactgca
     8641 aatcttttta cgcctccgta gagtgcgttg aaaaggatct tgatcccctt acaactaaat
     8701 tagtagtcat gtcctatccg tccgattcga cagaaacacg gggtagcgga ttaatcttgg
     8761 cttccagtcc tgcagcaaaa aaagcttacg gcatcaccaa tatcagccgt gccagagatc
     8821 ttcccttccc ctatccaagt gatttatata ttgttgcccc aaggatggcc tattatatgg
     8881 aaaagaacag agaaatcaat tctatctata aaaaatatgc agacgaagcg aaccatcatg
     8941 tctattccgt cgatgaaagc ttccttgatg taacaaatag catggcactc tttggtaaaa
     9001 caaatgcgta cgacatggct aaactgatcc ataaagatgt gtttgagcag accggaatct
     9061 acacgactat aggaattggc gataacccgc tacttgctaa actagcctta gacaatggct
     9121 caaaagacgg aaagcaaatg tttgctgaat ggcgttatga agatgtacca gaaaagttat
     9181 ggaaaatctc agatatcact gacttttggg gaatcggcaa acgaacagca aaacgtctga
     9241 aacaaatggg tatacattct gtttatgatt tagctcacgc ggattattat cacttgaaaa
     9301 gcaagctagg ggtcatcggt actcagcttt atgcccatgc ttggggcatc gatcgaagtt
     9361 ttttgggaca agaatataca ccaaaatcaa aaagtatcgg aaatagtcaa atcctagata
     9421 gagattatac aagacgagaa gaaatcgaga tcgttatcaa agaaatggcc gaccaagtcg
     9481 gcactcgctt gagacgatct gcagcaaaaa cacaagtagt cagcttatgg gtaggatact
     9541 ctttaggcta tcttgatcca aatggcaaga ccgggtttca ccagcaaatg aaaatcgaag
     9601 cgacaaatgc tagcagagat ctagcagatg ctttattgac cattttccac cagcattacc
     9661 actaccagga tattcggcgt gtaggcgtga actgttccga cctcgtatac gcgacaggtt
     9721 tacagcttaa cctttttgaa gatcctgaaa agcagataaa tgaagcgaaa gttgattttg
     9781 ttgtcgatac gattagaaaa aaatacggct ttcaatcaat catccatgcg catagtctct
     9841 tgcaaggagg acgcgccatc gcacgtagtt ctctcgttgg cgggcatgca ggtggtatga
     9901 gtggattaga aggtggaaaa gatgagacgg acagcaaaaa aattcattga ctataacgag
     9961 tatcgagatc ggccatttgg attaaaatgg gggactgctt ttgcgatgga agaactagtc
    10021 aacggtatca aaaaaaatga agaagaagca cgcaaacagc ctcttcctaa acaacaaatg
    10081 acattgcaag aaatcgatag tatactgaca gaagcctttt tatctcgtaa agcagtaatc
    10141 attcagctca atttaaaaga tgaatttgga agactcttgg atgaggtcga agggcacttc
    10201 attggtgaag cctatgaaga ttactttatg attgaccatc agccgatatt gtgggaagac
    10261 gtacggtata ttgagttaaa ggaaacaaaa aaatggtttg tgacatgaga ggttgtgaca
    10321 gaagtgatca acttcaaaaa ataagaaagg atttttcgaa aattgcttct taaatttttg
    10381 tggaattttg gcttattttt cgaagaagca acttctggaa caacgtatat tagatatcag
    10441 aggttgtgaa aaagtcgctt agttttgagt aacaagccaa ggcgctgatt tcacaacctc
    10501 tgtctatcta tccatctatt cggtaagatt catgcatgac gtcttcttta tttagtatgt
    10561 agagcccttt ttcttctgac cattcaacat aaatcagctg ttttacatct gcatagcctt
    10621 ctttcttcaa gttctccata agccatgctt cagtcaattg attggactga agcactttat
    10681 gctgaatttg cccttcatct acaagcagga tcgataaagc tttgtcttca tctgattttt
    10741 tcaatacact catttgaccg gcattctcta ggataacatg tttggcattt tcaagaaaaa
    10801 agcattcttg ctgtcttaag attgctcgca actgctccat ctcgatatgg ttatttgtca
    10861 actctgtcat gttgatgacg ccgtctttga ttagaacaga tggctcgcct ttgacccaac
    10921 gattgacctt ttcatttttt tggacgatac gttcgatacc ataaatcatt actgcccaaa
    10981 gcgcaatggc aaataggaca tgccctacat ggacattgtc atcataggtt gattcttcta
    11041 agatcccacc taaaacaagc gtatagacta aatcaaacgg tgtgatttct gacatggatt
    11101 tcttcccaag caaacgtacc actaataata aaccgatcaa cccgaacacc agtttcattg
    11161 taattgaaaa aatcattttt tctcccccta actatctgtt aattaaatta tatatgcttt
    11221 ctcacgaaca cgcatggaat atacaaggaa gaaatgtatc cttttactaa aaaaaggact
    11281 gtgaca
//
LOCUS       DACEUZ010000089        11268 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.089, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000089 DACEUZ010000000
VERSION     DACEUZ010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11268
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.089"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(808..1122)
                     /locus_tag="IXG44_001936"
     CDS             complement(808..1122)
                     /locus_tag="IXG44_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1658649.1"
                     /translation="MKHPPKTTVEQLAESSGLSDKTIQRMRNGEAVVIQSIVAMCIGL
                     HLHPDISTEMLQKLGYTLGPAVEIHMIYKTLLCNCKTMTIEECNGILTNADFEPLTKA
                     EV"
     gene            complement(1289..4279)
                     /locus_tag="IXG44_001937"
     CDS             complement(1289..4279)
                     /locus_tag="IXG44_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAQ1658650.1"
                     /translation="MKIQFDTLDYQTDAVNSAVRVFEGQTIKESNFTITDDYSRGSMF
                     TEDGIGVGNRVIINEEQMLKNVNKTQILDGIVPSDNLFGNNKAFPQFNIEMETGTGKT
                     FVYLKTILELNKQYGFLKFVIVVPSIAIKEGVLKSFEITKDYFKNQYNGVVYDLFMFD
                     SSKLDRVRSFASANTIDIMVTTIQSFNKDTNVMNRENDQLSGARPIDLIAETRPIVII
                     DEPQSVDNTDLAKDALKSLNPSAGFRYSATHRDNTYPKIYQLGAVDAYDNQLVKQIEV
                     AGIEMDEDGNRAHLKLIDVKTGKNGISAKIEVYKKTRNDADKITMNFKAGDDMFSKTK
                     LQAYDEVGFIQDIDATPGFESVTFSGNPEKITLESATLEDEAIKRAQISKTIEEHLNK
                     ELKFKQSGQRIKVLSLFFLDKVENYRVYDEEGNPSLGRYARIFEEEYERLIHLSKYKE
                     LNDVNVPVSEVHDGYFSADNKGKLKNTKGDTQADESTYEMIMKDKEGLLTFYDEEKHH
                     TAKANKIRFIFSHSALKEGWDNPNVFQIATLIETKDTINKRQKIGRGLRIAVNEDGER
                     VPGFAVNTLTVMANESYKDFAEGLQKEYEEDGVIFGVFNKDSFATIITHYDEVTEEME
                     ILGKEKSEQLFNYLKQEEFINSSGRATDKLKQAITDQEIEVPEEFIDVVPQILVAAKS
                     KVKSLDIKDAKDKVEVKVVKEALADDFLELWNKIKYKTTYKIHFDSNKLIEFAAKGNE
                     SFDGVDSIQTRKGTYLYKKGMVEMSQAGVKADESGESSVRATTSGMYKLPDIITFLQN
                     ETSLTRKTIVAILQSVNNLDSFKNNPLSYMNQAAKIINAIKNQLIVDGIEYTRTNDEY
                     EQTLFSTETLNAYLGEKGNSVKVDESKQKTVYDYVVTDSNIEKDFARKAEMDDNVKFY
                     IKLPDWFKIRTPLGPYNPDWALLYEEDDEQHLYFIVETKGDTSFEQLRPHERAKILAG
                     EKHFKAVDTDIKFKRVSDEAEIRG"
     gene            complement(4300..6144)
                     /locus_tag="IXG44_001938"
     CDS             complement(4300..6144)
                     /locus_tag="IXG44_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HAQ1658651.1"
                     /translation="MINENNQVPSSSLDITEQNIEKLKELFPEVLTEKKIDFDKLRLI
                     LGDEVETAPERYSFTWNGKKQAMQLAQQPTVATLKPNKAKSKNWDETQNLYIEGDNLE
                     VLKILQKSYANKVKLIYLDPPYNTGSDFVYKDSFSDSLENYLEVTCQVDDEGKKFSTN
                     SDTDGRYHTNWLNMMYPRLKLARNLLRQDGVIAISIDDNEVAKLRNLLDETFGENNFI
                     QEIAWRRSDNQANIGLAARVKEYILIYAKNKKELVYGRLPLSESAIREYRYSDSYAGK
                     FRRGILLDKTRGRYSYPVTTKSGNVLNGPWMVAEEKFKELDENNGIYWTSGGEEQPYS
                     KIYLSESKGAIPNDFWGIEYGTNQRASLEVEVLFGERYFDFPKPVSLIKSLISLGAPD
                     KDDIVLDFFSGSGTTAQAVMESNIDDDGKRKFILVQLPEDIDAKHKAYKAGMRKIPEI
                     AEKRISLAGDKIIAENPELAGKLDVGFKVFELSKSNIKKWNAEPKDLNEQFELLANNF
                     EEGSKPIDVVYEIMLKQGLDLTYPISETKVGEAVVYDIAFGAMFVVLGDKITSEVASH
                     IIKQIADEEAENSVVVLQDEKFINDSEKLNTIEQLNASGIQYNDILSI"
     gene            complement(6172..6894)
                     /locus_tag="IXG44_001939"
     CDS             complement(6172..6894)
                     /locus_tag="IXG44_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4391 domain-containing protein"
                     /protein_id="HAQ1658652.1"
                     /translation="MSELETKDIIKWWKFPSATIINRNLPKTQIYPHMKNAADKQFLQ
                     DSVQSIYMLASLKTDNTHIAVYEDDKELYQEIQFLYVKTKDKGDSTKIYKMLAHLIPY
                     PLVILTDEPDSFTIYTGRFEKLSTGFLKLMNVYPSPVYQDENLEEVLQQISLIDLPRQ
                     NLKTFYDGLRDEIVTATAKSQYGEAVANITGEEKDQLDALKKQIEDLRGQIKKERQLN
                     RKIDMQMKLKKLKDELSSKINQ"
     gene            complement(6895..10152)
                     /locus_tag="IXG44_001940"
     CDS             complement(6895..10152)
                     /locus_tag="IXG44_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002403337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAQ1658653.1"
                     /translation="MELKLIDNKGDNTLENLISEKVTKHSKISIQTAAFSIFAFHALQ
                     KEIKRSKGLRLVLTKSFFEKEETSFLKRYEIAQSEEDISGNKYEIKLRNKMNTAFIAR
                     ETARLIEEKVKVHQLGPNQNALDELLIENEADIEGNVVVPSMFKFTSDGLGLTESNNV
                     SSMTGIIGEPNHLAGLKADFDAVWNDPKKSKEVTDKVLEKVRTIYQENPPEWIYFVSL
                     YHIFHDKLSELDEQDIAPEGSGFKDSVIWNKLYQFQKDGVMGLIRKLEKYNGAILADS
                     VGLGKTFSALAVIKYFELQRKRVLVLAPKRLRDNWTLYTRPDVRNVLVDDYFNYTVLN
                     HTDLSRYKGESDGIKLDTFLWSDFDLVVIDESHNFRNNDTSVNRETKTRYQRLMEDII
                     QKGRKTKVLMLSATPINNRMNDLKNQIGFITEGKTDALAKYDVDDIDTTLRLAQQAFN
                     QWMELDESERTTQSFLDKVNPDYFKLLDMLTIARSRKHIEKYYDTTSLGDFPERLKPM
                     TVKPNIDSSGQFPNLHDVYDSIGYLNMSMYQPLQYVLSTKRKRYEELYDTKVKEGKSS
                     FRQVDREQSLAKLIRANLFKRLESSIYSFGSTLDRMVGGIEDILEKIKEHQAGNVEMP
                     SITDFDDEDLESAFDEQAVGTKTKILIGDMDLVKWSQALNEDLEKLKELQTVTHRVTP
                     DKDDKLMDLEKILKHKYEHPINGNNKKVIIFTAFADTANYLYENLADKVKRLYGLNSA
                     LVTGGSTPNKTTMKGVAVNDMSDILTNFSPISKGRDQINPNATEEIDLLIATDTISEG
                     QNLQDADYLINYDIHWNPVRVIQRFGRIDRIGSRNKQIQLVNFWPNVDLDTYLNLEQR
                     VKGRMVMLNTAATGEDDLLNIKSNKEMNDLKYRKKQLEQLQTQVIDLEDVNGAISITD
                     MTFNDFKADLQNALKEHEKLLNEAPLGMYAITDTTNFPEAEPGVILTLKQQTNDLGQE
                     NSILPYIVVYMRMDETVKINYMYAKQVLDFFKKLTVGKKTVNQPLVDEFYEETNGGKD
                     MSAYSEILSKAIEAIRGKQDEVGMGSLFSPGGTSVQTELLDDLDDVELISFLIIR"
     gene            complement(10201..10401)
                     /locus_tag="IXG44_001941"
     CDS             complement(10201..10401)
                     /locus_tag="IXG44_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002403339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1658654.1"
                     /translation="MMSYKKLWKLLIDRDMSKKDLAELADLSQYTINKLNNSGTVTTE
                     TLTKICIALNCDVGDICEVVAA"
BASE COUNT         3252 a         2363 c         1776 g         3877 t
ORIGIN      
        1 aagtaaatct atttttttaa acgtagattc acaatttcat taaatttttg taatgtttcc
       61 tcatcaggta aaataacttc tagttcgatt atcatccctt tcgttaaata cttaataatt
      121 gaccctgaag atctagaagt aattttattt aaatattagt aatcaaacat tttatttgag
      181 ttcgattcta ttatataatt tcgatgataa gcttcaaatt taccctcgaa tctattaaac
      241 caacactctc catttccaac cagtatcatt gcctttgtat caaaagagta tgatggatat
      301 tttagatgtt ctgagctaaa aatgtaacat ggatattgac catcttaggt agcgtgatta
      361 gcatcttttt tgccggttgt tattgttgtt aaatccccta atttttttcg attccattct
      421 ttcggtagga cttgtttatt tttagaagct agttgtccat cttcactagt taaattggaa
      481 tctacaaacc atttatgaaa tgtcgaaagg tatagaaaat aagcattttt actggggtta
      541 accagcagta atgttctatt tctattcctt gcccttattg caaatcgggc tctgaatatt
      601 aatttttatc gtgactaatt ccttagtact cccttatcat atctagattg agcgctgtaa
      661 accatgacac ttactgtccg ttaatttcgt ctaaaacacg caaaaaaaga cctgattcaa
      721 agatataaaa tctctaaatc aggtcttcat tgagtttaca aaccggacag taagtgtctg
      781 attctcataa aaataaattt aaaatcttta aacttcagct ttggttaacg gttcgaagtc
      841 agcattagtc aaaattccgt tgcattcttc aattgtcatc gttttacaat tgcaaagcag
      901 cgttttataa atcatatgaa tttctaccgc tggccccaac gtatatccga gcttttgaag
      961 catttctgta ctaatatcag gatgaaggtg caaaccaata cacattgcga caattgattg
     1021 aataactaca gcttctccat ttcgcattcg ctgtattgtt ttatcagaaa ggccagatga
     1081 ttctgccagc tgttcaacgg ttgtctttgg tggatgcttc atcaacacct taagcgcatc
     1141 accgaagcta agtggcgcaa tttcattcta tacttgtttt aaaccttttt gatctttctt
     1201 caattaaact gatgcatatt aacatagttt ttaaattaac gttgctgtac cattgattta
     1261 cttctccctg ttttatttca aatatagatt acccacgaat ctccgcttca tcagaaaccc
     1321 gcttaaattt aatatctgta tcaactgctt taaagtgttt ctcaccagcc agaatcttag
     1381 cccgttcatg aggacgtaac tgttcaaaac ttgtatcacc tttcgtttca acgatgaagt
     1441 ataggtgttg ctcatcatct tcttcatata gcaatgccca gtctggattg taaggcccaa
     1501 gcggtgtgcg aatcttgaac caatctggca gcttaatgta gaacttaaca ttgtcatcca
     1561 tttcagcctt acgagcaaaa tccttttcaa tgtttgaatc agttacaaca tagtcataaa
     1621 cagtcttttg tttactttca tcaactttaa ccgaattccc tttttcaccg aggtaagcat
     1681 tcaaggtttc agtgctaaac agcgtctgtt catattcatc atttgtacga gtatattcaa
     1741 taccatccac aatcaattga ttcttgattg cgttgataat cttcgcagcc tgattcatat
     1801 atgacaatgg attgttctta aaactatcca gattgttgac tgactgcaaa attgcgacga
     1861 ttgtcttgcg agtcaaactt gtttcatttt gcaagaaagt aatgatatct ggcaatttgt
     1921 acataccact cgttgttgca cgaacactgc tttcgccaga ttcatcagcc tttacaccgg
     1981 cttgactcat ctcaaccatc cccttcttgt aaaggtaggt ccccttgcga gtctgaatgg
     2041 aatcaactcc gtcaaatgat tcatttcctt ttgcagcaaa ttcgattaat ttgtttgaat
     2101 caaaatgaat cttataagtc gtcttgtatt tgattttgtt ccaaagttct aaaaagtcat
     2161 ctgctaaggc ttcttttaca accttgactt cgactttatc cttggcatct ttgatatcaa
     2221 gactcttaac cttagattta gcagcaacca aaatctgtgg cacaacatca ataaattctt
     2281 ccggtacttc aatttcctga tcagtaatcg cttgtttcaa cttatcggtt gctcgcccag
     2341 atgaattaat aaattcttcc tgtttgaggt agttgaacaa ttgctcagat ttttctttgc
     2401 caagaatttc catttcttcc gtaacttcat cgtaatgcgt aataatcgtc gcaaagctat
     2461 ctttattaaa tacaccaaag ataacaccat cttcttcgta ttctttttga agaccttcag
     2521 caaaatcctt atagctttca tttgccataa cagtcagcgt gttaacagca aatccaggga
     2581 cacgttcacc atcttcgtta accgcaatac gcaggccacg accaattttc tgacgtttgt
     2641 taattgtatc ctttgtttca atcaaagtgg caatctggaa tacattcggg ttgtcccaac
     2701 cttccttcaa agctgaatga gagaaaatga agcgaatttt attagccttc gctgtatgat
     2761 gcttctcttc atcatagaaa gtcagtaagc cttccttgtc cttcataatc atctcgtagg
     2821 tagattcatc ggcctgagta tcacccttgg tattttttaa cttaccttta ttatctgctg
     2881 agaaataacc atcatggact tcggatactg gcacattcac atcgttcaat tctttgtact
     2941 tggacaaatg aatcaagcgt tcatattctt cttcaaaaat acgagcataa cggccaagac
     3001 taggattacc ctcttcatca taaacgcggt agttttcaac cttatccaag aagaacaaac
     3061 tcaacacctt gattcgttgc cccgattgtt tgaacttcaa ctccttgttc aagtgttctt
     3121 caatcgtctt agaaatctga gcacgtttta ttgcttcatc ttctaatgta gctgactcaa
     3181 gcgtgatttt ttcaggatta cccgagaaag taactgattc aaatccaggt gtagcatcaa
     3241 tgtcttgaat gaacccaact tcatcatacg cctgaagctt ggttttacta aacatatcgt
     3301 caccggcttt aaagttcata gttattttat cagcgtcatt acgtgtcttt ttataaactt
     3361 caatcttggc actgataccg ttcttaccag tttttacgtc aatcaatttc aaatgagcac
     3421 ggttaccatc ttcgtccatc tcgatgccgg ctacctcaat ctgcttaacg agttgattgt
     3481 cgtaagcatc aaccgcacca agttgataaa tcttcggata agtattatct cggtgagtgg
     3541 ctgaataacg gaagcccgct gacggattta aactcttcaa agcatcttta gccaagtcag
     3601 tattatcaac agactgtggt tcatcaataa taactatcgg ccgtgtttca gcaatcaggt
     3661 caattggtcg tgcaccagaa agttggtcat tttcacggtt catgacgttt gtatctttgt
     3721 tgaaagattg aatggtcgta accatgatat caatcgtatt tgccgatgca aatgaacgaa
     3781 cacggtcaag tttactgcta tcaaacatga ataaatcata aaccacaccg ttgtattgat
     3841 ttttgaaata gtcttttgta atctcgaagg atttaagtac cccttcctta atcgcaatac
     3901 ttggcactac aatcacgaat ttcaaaaatc cgtattgttt gtttaattca agaatcgttt
     3961 tcaagtaaac aaaagtctta ccagtccctg tttccatctc aatattgaac tgcgggaaag
     4021 ctttgttatt tccaaacaga ttgtcacttg gcacgattcc atccagaatt tgagttttgt
     4081 tgacgttctt caacatctgt tcttcattaa taatcacacg gttaccaaca ccgattccat
     4141 cctcagtgaa catcgaacct cgtgaataat catctgtaat cgtaaaattt gattctttaa
     4201 ttgtctgacc ttcaaacacg cgcaccgcac tgttaacggc atctgtttga tagtcgagtg
     4261 tatcaaattg tattttcaag agtctttacc tcctttgctt taaatactta aaatgtcatt
     4321 gtactggatg ccacttgcat tgagctgctc aatcgtattt aacttctcac tatcattgat
     4381 gaatttctca tcctgtaaaa caactactga gttttcagct tcttcatcag caatttgctt
     4441 aataatatga cttgccactt cgctcgtaat cttgtcacca agtactacaa acattgcacc
     4501 aaaggcaata tcataaacaa ccgcttcccc aacttttgtt tcactaattg gataagtcaa
     4561 atcaagacct tgcttcaaca tgatttcata aacaacatcg attggttttg aaccttcttc
     4621 aaagttgtta gccagtaatt caaactgctc attcaagtct ttgggttctg cgttccattt
     4681 cttgatattt gatttagaga gttcaaaaac tttgaaacca acgtcaagtt ttcctgctag
     4741 ttcaggattt tcagcaatta tcttgtcccc ggctaaagag atccgttttt ctgcaatttc
     4801 tggtattttc cgcattcctg ccttgtatgc tttatgtttt gcgtcaatat cttccggcaa
     4861 ttgaacgaga ataaactttc tttttccatc atcatcaata ttggattcca ttaccgcttg
     4921 tgctgtagtt ccagagccag aaaagaagtc taggacgata tcatctttat ctggtgcacc
     4981 aagtgaaatc aaacttttga ttaatgaaac aggctttgga aagtcaaagt atctttctcc
     5041 aaataaaacc tctacttcta aactagctcg ttgattcgtt ccatattcaa ttccccaaaa
     5101 gtcattcggt attgcacctt tactttcaga caagtaaatt ttgctatatg gttgttcttc
     5161 accaccgctt gtccagtaaa tgccattatt ttcatctagt tctttaaatt tttcttccgc
     5221 aaccatccaa ggaccattta aaacatttcc agattttgtt gttactggat aagagtagcg
     5281 tccgcgagtt ttatccagta aaattcctcg tcggaacttt cctgcataag aatcagagta
     5341 gcgatactcc cgaatcgcac tttctgataa aggcaatcgt ccataaacta attccttctt
     5401 attttttgca tagattaaaa tatactcttt cactcgtgca gctaaaccaa tattagcctg
     5461 attatcagag cgtcgccatg caatctcttg aataaagtta ttttcaccaa aagtttcgtc
     5521 aagtaaattt ctaagctttg caacttcatt atcatcaata gaaattgcaa taactccatc
     5581 ttgtcttaaa agatttctag ctaacttaag acgtggatac atcatattca accagttagt
     5641 atgatatctt ccatctgtat ctgaattagt tgaaaacttt ttcccttcgt catcaacctg
     5701 acaagtaact tctaaataat tctcaagcga atcagaaaaa ctatccttat acacaaaatc
     5761 actacccgtg ttgtagggtg gatccaaata tatgagtttc actttattgg cataagactt
     5821 ttgcaaaatc ttcaacacct caagattatc tccttcgata taaagatttt gtgtttcatc
     5881 ccagttcttt gactttgcct tatttggctt caatgttgcc accgttggct gttgagccag
     5941 ctgcatcgct tgtttcttgc cgttccaagt aaaactgtag cgttcgggtg ctgtttcaac
     6001 ttcatcccca agaatcaaac gtaacttatc aaagtcgatt ttcttttcag ttaatacctc
     6061 aggaaataat tccttcagct tttcaatatt ttgttcagtg atatcaagag aacttgaagg
     6121 cacttggtta ttctcattta tcataaatag gatttccttt cacactttca cttactgatt
     6181 aatcttgctt gaaagctcat cttttaactt tttcagcttc atctgcatat caattttgcg
     6241 attcagttga cgttcctttt taatttggcc acgcaaatct tcaatctgtt tctttaatgc
     6301 atcaagctgg tctttttctt caccagtgat attagcaact gcttcaccat attgtgactt
     6361 agccgtagct gtcacaatct catcccgaag tccatcataa aaagttttca gattctgccg
     6421 tggtaaatca atcagtgata tttgctgcaa cacttcttcc aagttttcat cttgataaac
     6481 cggggatgga tacacattca tcaacttcaa aaagcctgtt gataacttct caaagcgacc
     6541 cgtgtagatc gtaaaactat ccggttcatc ggtgaggata actaatggat atggaatcag
     6601 gtgtgccaac attttataga ttttagtcga gtcaccttta tccttggttt tcacataaag
     6661 aaactgaatt tcttgataaa gttccttgtc gtcctcatac acagcaatat gtgtattgtc
     6721 agttttcaga cttgccaaca tataaatgct ttggacggaa tcttgtaaga attgcttgtc
     6781 cgccgcattt ttcatatgtg gatagatttg cgtttttggt agatttcgat tgatgattgt
     6841 agcacttgga aatttccacc atttgattat gtctttcgtt tccaattcgc tcacctacct
     6901 aataatcaaa aatgaaatta actcaacatc atctaaatca tccaatagtt cagtttggac
     6961 gctagtacca ccaggtgaaa acaatgaacc catccctact tcatcttgct tacctcgaat
     7021 tgcttcaatc gctttactca agatttcaga ataggcactc atgtccttac caccattcgt
     7081 ttcctcataa aactcatcta caagtggttg gttgaccgtc tttttaccga ccgtcagctt
     7141 tttaaagaag tccaacactt gcttggcgta catgtagttt attttgactg tttcatccat
     7201 tcgcatataa acaacaatgt acggcaagat actgttttct tgtcccaagt cattcgtctg
     7261 ctgcttcaag gtcaatatga cacccggttc agcttcaggg aagttggttg tatccgtaat
     7321 tgcatacatg cctagcggcg cttcattcaa caacttctca tgctctttca aagcattctg
     7381 caagtctgct ttgaagtcat tgaaggtcat gtcggtaatg gaaattgccc cattgacgtc
     7441 ttccaaatca atgacttgag tttggagttg ttccaactgt ttcttacggt atttcaagtc
     7501 gttcatttct ttattagatt taatattcaa taagtcatct tcaccggttg ctgccgtatt
     7561 taacatgacc atacgtccct tgacacgttg ttccagattc agataagtat ccaagtccac
     7621 attcggccag aagtttacta gttgaatctg tttattacgt gagccaatac ggtcaatacg
     7681 cccgaaacgt tgaatgacac gcactggatt ccagtgaata tcgtagttaa tcaagtaatc
     7741 tgcatcctgc aagttttgcc cttcagagat ggtatccgtt gcaatcaaca aatcaatttc
     7801 ttcggttgcg ttcggattga tctggtcacg cccttttgat atcggtgaga agttcgtcaa
     7861 gatatcactc atatcattga cggccacacc tttcatggtt gttttgttcg gtgtagaacc
     7921 acccgtcacc aatgcagaat tcagaccata aagtcttttt actttgtcag ctaagttctc
     7981 atagagatag ttagccgtat ctgcaaaggc agtaaagatg attactttct tgttgttgcc
     8041 attaattgga tgctcatact tatgcttgag aattttctcc aagtccatca acttatcatc
     8101 tttgtccggt gttactcgat gcgtgacggt ttgcaattct ttcaactttt caagatcttc
     8161 attaagagct tgggaccact tcaccaagtc catatcacca atcaatattt tagtttttgt
     8221 ccccactgcc tgttcgtcaa aggcactttc caaatcctca tcatcaaaat cagtgatact
     8281 tggcatttcc acgttacccg cttgatgctc tttgattttt tcaaggatat cttcaatccc
     8341 gccaaccatt cggtcaaggg ttgatccaaa cgaataaatg gaactttcca atcgtttgaa
     8401 caaattggca cgaatcaatt tagccaagga ttgttcacgg tcaacttgcc tgaatgacga
     8461 cttcccttct tttaccttgg tgtcatacaa ttcttcgtag cgctttcttt tagtcgagag
     8521 cacatactga agtggttggt acattgacat gttcaagtaa ccgattgaat cataaacatc
     8581 atgtaagtta gggaactgcc ctgatgaatc aatatttggt ttcactgtca ttggcttcag
     8641 gcgttcaggg aaatctccca atgaagtcgt atcataatat ttttcaatgt gtttccgact
     8701 acgagcaatc gtcaacatat ccagtaactt gaaataatcc ggattgacct tatcaagaaa
     8761 gctttgagtc gtccgttcag attcatccag ttccatccat tgattaaaag cttgttgtgc
     8821 taaacgcagt gtcgtgtcaa tatcatcaac atcatacttc gccaacgcgt ccgttttgcc
     8881 ttcagtgatg aaaccaatct gatttttcaa gtcattcatt cggttgttga ttggtgtagc
     8941 tgaaagcatc aatacttttg tcttacgtcc cttttgaatg atatcttcca tcaagcgttg
     9001 ataacgtgtt ttggtttcac gattgacgct ggtatcatta ttacggaagt tgtgggattc
     9061 atcaatgaca accaaatcaa aatcagacca cagaaaagta tccagcttga ttccgtcact
     9121 ttcaccttta taacggctaa ggtcagtgtg gttcaaaaca gtgtagttaa agtagtcatc
     9181 cactaaaaca tttcttacat caggtcgtgt ataaagtgtc cagttatccc gtagtcgttt
     9241 tggagcaaga acgagtactc gtttacgttg caactcaaaa tatttgataa cggccaaggc
     9301 tgagaaagtt ttacctaaac caactgaatc tgctagaatc gcaccgttgt atttttcaag
     9361 cttacgaatt aagcccatca caccatcttt ttgaaattgg taaagcttat tccaaattac
     9421 tgaatccttg aacccagaac cttctggcgc aatatcttgc tcatccaatt cactcaactt
     9481 gtcatggaaa atatggtaaa gcgatacaaa ataaatccat tcaggtggat tttcttgata
     9541 aatggttctg actttttcaa gcaccttgtc tgttacttct tttgactttt tagggtcatt
     9601 ccagacagcg tcaaagtcag cttttaaacc agctaagtga ttaggttcac caataattcc
     9661 cgtcattgaa gaaacgttat ttgactctgt aagtcctaag ccatctgaag taaatttgaa
     9721 cattgaagga actaccacat taccttcaat atctgcttca ttttcaatca gtagttcatc
     9781 caaagcattt tgatttggtc ccagctgatg aacctttact ttttcctcaa tcaaccttgc
     9841 cgtttcacga gcaataaaag ccgtattcat tttatttctg agcttaattt catacttatt
     9901 tccagaaata tcttcttcac tttgcgcaat ttcataccgc tttaaaaaac tagtttcttc
     9961 cttctcaaag aaagatttcg tcagaaccaa acgcaacccc tttgaacgct tgatttcctt
    10021 ttgtaatgcg tgaaaagcaa aaattgaaaa tgcagcagtt tgaatactga ttttcgaatg
    10081 ttttgtcacc ttttcactga ttaaattttc tagggtgtta tcccccttgt tatcaattaa
    10141 ttttaattcc atgagcgtct cccacaaggc tataatttta attctttatt ctgtgctgta
    10201 ttaagcagct accacttcac aaatatcccc aacatcacaa ttcagtgcga tgcaaatttt
    10261 agttagtgtc tcagttgtca ctgtgccact attatttaat ttgttgatgg tgtattgact
    10321 aagatcagcc agttcagcta aatctttctt tgacatgtct cggtcaatta ataacttcca
    10381 caatttttta tagctcatca tcttatcacc tattttctct acaatctgat atgccgataa
    10441 taaatatatt atatcacgat tgatatgaaa ttttacataa aaatatagcg aacacttaat
    10501 tattttacga ttgattagat tttgataaat atttatagga acgtaaattt tagggggtgt
    10561 ttaggggtgt catagggtgt taattcaaaa cctaggggtg ttatattttt atccccttgg
    10621 aaattatgcc tgtatctcat tgatgaaatt catcgtaaaa taaaaaagtt tctcaagcca
    10681 caagctcaag aactcccgta ttatcgcctt ttctagcagt ttgtatttgt atcgattgcg
    10741 tggttttaat atcaatgtaa attagcttta cctttggaaa gcattaatag aaatgactga
    10801 agctagtaag gaactggcag ctaagttaac tgaaaatagt tatttagctt atattaatga
    10861 aaaatccaca cgagaattat ggacactcga tgactaacaa tatccttatt agtatctatc
    10921 cacgatacat ggaaaaaatt ataaacaaac aaaaaaactt tgaatttaaa atttctatct
    10981 tacctcagct ccttataata tcttttggat ttacgaaaca atcccaccaa agctttaaag
    11041 tataaattga ttgtaaaaaa tccaattact aaacttaaga aaaatcaaca ctacttttta
    11101 ggagacgatg agtttgaaca attagtttaa aagggaaaat atgcttacga aattatccac
    11161 ctagaagaaa tacttttacc aataagtctg tctaatttaa aaaatttagg agttactgca
    11221 ccacaaggat atgcatatat aaaaaaatat cctagtcttg tcgatatt
//
LOCUS       DACEUZ010000090        11253 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.090, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000090 DACEUZ010000000
VERSION     DACEUZ010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11253
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.090"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(40..1419)
                     /locus_tag="IXG44_001942"
     CDS             complement(40..1419)
                     /locus_tag="IXG44_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HAQ1658655.1"
                     /translation="MTKVTLKKVNPPKKDNEAKIYQLTDETMKFRKEKVLAAMEKSEI
                     DTLVIYCDLEHGGNFSYLTGFVTRFEESLLVLHQTGEAFLVLGNENTKMVDYSRIEAE
                     LIHTPLFSLPDQPMDNEKPLTEVLTNAKISKGKKVGLVGWKMFTSEEDNYQIQDLPAF
                     IVSSIVELVGEDAVSNRTDLFIHSDYGVRSLNTANEVAYYEFGSSLASDCVMNAIDEV
                     EVGKTEMEIGNHLVKYGQPNNVIPIAATGERFENAYIYPTDKQVKLGDKMSITTGFKG
                     GLASRSGYAVATTDDLPEGQKDYIEKVAAPYYDAVVAWLENIKIGMQGKELYSVIGKV
                     LPKSEYKWHLNPGHLTADEEWMSSPMKKDSEITLKSGMLLQIDIIPSVAGYAGASCES
                     GIALADESLRDQLAKEYPNVWSRIISRQEYIRNELGIELPDEVLPLSSGVGYYTPFFL
                     EKNLALVKG"
     gene            complement(1440..2987)
                     /locus_tag="IXG44_001943"
     CDS             complement(1440..2987)
                     /locus_tag="IXG44_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1658656.1"
                     /translation="MGPLSIIVASGLLLGIVSILQNPTLVGESFANATVIQGFVGGVQ
                     AIVGTMFGLLPILFAISVAIGMSREDKEIAAFSVVIAFILFHVVINYLLQLRGITAES
                     TSVETLMSDGMSQIKAVETSNMYETLLGTFTYRMGVFGGIIAGLWTAMIHNRYHTKQL
                     PAAFSFFSGNRFVPIMIILTIPIISIASFFIWPFVNTAINALGGLIGKSGVIGTFLYG
                     FSERLLIPTGLHHVLNQLIRFTPIGGTATINGETVSGALTIFNTELALPVKDMDILRS
                     ATRFLTQGTHPFMVFGLPAACYAMYKTSYLAQRPKIKGMLIAAAATCFVTGITEPIEF
                     AFIFISPILWVFHAFMAGLSFMLMNLFGVAVGNAGGGFIDLAIFGILQGTYTRWYLVV
                     ALGLVYAVAYYFVFKYVIERFDVKTPGRSNDDVLEDQDEEMTENELGKLILQGLGGAG
                     NIQEIDNCISRLRLVLKDTATIDEHILKKTGSMGIVKINENNLQVVYGGQVENATRSL
                     KNEMKRK"
     gene            complement(3236..4015)
                     /locus_tag="IXG44_001944"
     CDS             complement(3236..4015)
                     /locus_tag="IXG44_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ1658657.1"
                     /translation="MTLEQLVNEHHSKLNENDDYVLSFITQNMEISISQTVSELAKQA
                     NVSDSTIIRLTKKLGFKGYGEFRYYLKEEFARMQKTRVDKSSLFLPEVLLGDVQATTK
                     LFEDDKSVEDIYETIRKADKIFAYATGYGQRMMLNEFARCMWNLGIYIIVVPEKNELD
                     LISKALTSNDLLFVISLSGRVDRIDDTLRRIHMKGTQLVSVTRFGQNNLASLSDYSLY
                     YQVTNINDVNELNNSSFCTLNLALSLLYEGYINYYKQSSVD"
     gene            complement(4090..4548)
                     /locus_tag="IXG44_001945"
     CDS             complement(4090..4548)
                     /locus_tag="IXG44_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAQ1658658.1"
                     /translation="MGFFSRKEKLVAPVSGNLIPLSQVSDPVFSQGMMGEGFAVEPTN
                     AKVVAPIDGVIKSIFPTKHALTLTSNQGRDLLIHIGIDTVQLKGVGFQVLVEEGQRVS
                     SGESLVIFDCDQIKAEKLSDVVMVLFPEDKGIKLEVSEKKVSEGELLFKI"
     gene            complement(4560..5819)
                     /locus_tag="IXG44_001946"
     CDS             complement(4560..5819)
                     /locus_tag="IXG44_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HAQ1658659.1"
                     /translation="MKTNFERIKKDIENLAEYNATPGEGVTRSAYSKEDREARNYLIN
                     QMEQLGLEIWEDGFSTLFGRRAGKKADAPSVMIGSHYDSVVNGGRFDGVAGVVTALET
                     MRILEENNFENDYPIDLILMNAEEGETFGPSTGVSNSRAMMGTLTMSELETVKNRHGQ
                     TKLEALRAYGVEPDLEAAKRDPKTIKNFIELHIEQGPVLEDEGKDIGLIEFIPGIGRF
                     KARFYGETEDSTAPMNKRKDALVAASKFVLFVEQWMKNLGEGIAGTVGKLTIKPNSNQ
                     FVPDFVEALIEIRTFDTKTTNPQEIKEKLLNELSVIQGETGVRTELEEMARINYSNPT
                     NPSVMDQGNVEKMKHICDQLGYSYQVINNGTGHDAMMMTDFVPTNMIYIPSKGGITHH
                     PDEWTDFEDVKKGADVLLNLVMDISKD"
     gene            complement(5935..6249)
                     /locus_tag="IXG44_001947"
     CDS             complement(5935..6249)
                     /locus_tag="IXG44_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ1658660.1"
                     /translation="MDYEIQKFISEKEDQYFDRKSARIKPRDIIKHIVAFANADGGKL
                     VIGIEDDGTISGFKFQGAIDPWICVNKVDAFIESFLNQSTLELCYETRKSSYGGIYDN
                     DF"
     gene            complement(6395..7891)
                     /locus_tag="IXG44_001948"
     CDS             complement(6395..7891)
                     /locus_tag="IXG44_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-directed DNA polymerase"
                     /protein_id="HAQ1658661.1"
                     /translation="MNHQEAKQFFLKTSSFFSTSLPNYIDLSVGIEKAVDLLKNKPFT
                     ELVRHKRSLSEAVDVNHRILANKDGNYAWRPLQILHPLVYVDLVNGITQKNNWNHIKN
                     KFEEFQADKRIECISLPVESISSKSDTAETILNWWENLEQAQISYALEYDYCIHSDIT
                     DCYSSMYTHTIPWAVHSKEWAKTHRKDTQGVGNLIDSKIRYLQNGQTNGIPQGNVLMD
                     FVAEIVLGYADKMLLNKIEKTEIKEFKILRYRDDYRIFSVRKDQAEVIIRLLSEVLSD
                     LNLKLNSNKTFLSDNIILDAIKPDKIYWDLKHASFTEKSNNKWKFKLSLQKHLLQVKL
                     LGDKYPNCGSLSKALSEVYTHRVSTLNKRPDDIYQLISILVNIMQKNPRTLEACIVIL
                     GKLFEFIAVEEINLYLDKILRKFVNTPNTDIVEIWLQRLSLIHSREKSYSAKLCKKVS
                     NPKGFTLWNSDWLKKGFDESGIVNETFISNLSLTTPAKDLELFISQYE"
     gene            complement(8069..8548)
                     /locus_tag="IXG44_001949"
     CDS             complement(8069..8548)
                     /locus_tag="IXG44_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3841 domain-containing protein"
                     /protein_id="HAQ1658662.1"
                     /translation="MKVYTVQPVDFLQSVDSEGFIYPKKRYTELSVFFDNPNAVGALH
                     WLEEEYTKRIQLPLDRDLIWLWRDTCHLKYARKDNFTDFSKYNFYCFEIPIEFYRKNI
                     LWSRFIEWHEPLNHEWETDENQEFEHIFDIPKHPGYGDIQGVTTRLHKDWIKKITHN"
     gene            complement(8924..9169)
                     /locus_tag="IXG44_001950"
     CDS             complement(8924..9169)
                     /locus_tag="IXG44_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1658663.1"
                     /translation="MLFLKEEKLIDKENVIGSNIRRIRLEKGMGQTELVRELQLSKIA
                     MTRETLVKIEGGRQHIKLDQLRAIRDILQVSYEDLLQ"
     gene            9274..10047
                     /locus_tag="IXG44_001951"
     CDS             9274..10047
                     /locus_tag="IXG44_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1658664.1"
                     /translation="MKKFAIILTCALLTGAISGCATSDTNSTSTETSTTTSEKNYPIH
                     FFPVPDGFEAYYHDKKLDVPLSESNSIEKVLPIEIEKNQENIKLKAELPILNSTEIDW
                     HENIKVTIEKGALAQLYTEKVEQFFESGANKDFSKISDYSDNFFNETQDEITNSTFMA
                     EDMYNMTLKKIKAYDHPLNLTLNNGLLSFVLAGYVDYEYSPDYLETPLQHENNDINFR
                     FVYESKSKTWLVDQLSTINTSFHELPKPENEADMVIKIF"
     gene            complement(10276..10524)
                     /locus_tag="IXG44_001952"
     CDS             complement(10276..10524)
                     /locus_tag="IXG44_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAQ1658665.1"
                     /translation="MDTITPTNFKKNIGKVLQEVVHSNSEIEITRDSKNGLNDGVIMI
                     SKRKWELLQEELYLQRTGTWAYVSDLMKNEKEDDFKVV"
     gene            complement(10623..10937)
                     /locus_tag="IXG44_001953"
     CDS             complement(10623..10937)
                     /locus_tag="IXG44_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1658666.1"
                     /translation="MADENKVVPLETLVINKEEFEQLFLDFFVQYDRIYDFSSSLDLF
                     ELEAEIIENSREENGKIIISVEKFNELFSETFEIDTDYYQPQEVFRLFRDQIIGISQT
                     TK"
     gene            complement(11074..>11253)
                     /locus_tag="IXG44_001954"
     CDS             complement(11074..>11253)
                     /locus_tag="IXG44_001954"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1658667.1"
                     /translation="NKNGEELYEYAMILNNPEYIESGKAETVNYYYELPSEIQNYQIV
                     IREADGYFVGLPFTL"
BASE COUNT         3500 a         2317 c         1684 g         3752 t
ORIGIN      
        1 tcatttcttc cgttttaaag aagaaatgat tttcatagat caacctttaa ctaaagctag
       61 attcttttct aaaaagaatg gtgtgtagta gcccacaccg ctggacaacg gtaaaacttc
      121 atccggcaac tcgatgccca gttcgttacg gatgtactct tggcgagaaa tgattcgcga
      181 ccaaacattt ggatattctt tggctagttg atcacgaagc gactcatcag ctagcgcaat
      241 cccactctca cagcttgcgc cggcatagcc agcaactgat ggaataatat caatttgtag
      301 taacattccg gacttaagag taatttctga gtcttttttc attggcgaag acatccactc
      361 ttcatcagcc gtaagatgcc ccggatttaa gtgccatttg tattccgatt taggcaagac
      421 tttgcctatt actgaatata gctctttacc ttgcattcct attttgatat tttccaacca
      481 tgcgaccacg gcgtcgtagt atggagctgc aactttttca atgtaatcct tttgcccttc
      541 tggtaggtca tctgtcgttg caactgcata gccacttcga cttgctaatc cgcctttaaa
      601 gcccgtagta attgacattt tatcgcctag tttaacttgt ttatcagttg gatatatata
      661 ggcgttctca aaccgttccc cagttgcagc aattggaatg acattatttg gttggccgta
      721 cttaactaag tggttaccaa tttccatttc tgtttttcct acctctactt catcaatagc
      781 gttcatcaca caatctgaag ccaaggatga gccaaattca taatatgcca cttcattagc
      841 agtatttaat gagcgtactc cgtaatcact gtgaatgaac aggtcggtgc gattgctaac
      901 ggcatcttcc cctactaatt ctacaatcga acttacaatg aatgccggca aatcttggat
      961 ttggtagtta tcctcttctg aagtaaacat cttccaacca acaagcccaa ctttcttacc
     1021 tttactgatt ttagcattgg tcaatacttc agttagtggt ttctcgttat ccatcggttg
     1081 atccggtaat gagaagagtg gcgtgtggat taattctgcc tcaattcttg aatagtccac
     1141 cattttagta ttttcatttc ctagaactaa gaaagcttca cccgtctgat gtaataccaa
     1201 caagctttct tcaaagcgag taacaaaacc tgttaaataa gagaagttcc cgccatgttc
     1261 caaatcacaa tagattacga gggtatctat ctctgacttt tccatcgctg caagtacctt
     1321 ttctttacgg aacttcattg tttcatcagt caattgatat attttagctt cattatcttt
     1381 tttaggtggg ttaacttttt ttagagttac cttagtcata ttgtcactac tttctgtttt
     1441 tatttacgct tcatttcatt tttcagagat cgagttgcgt tttccacttg tcctccatat
     1501 accacttgta aattattttc gttaatttta acgattccca ttgagccggt ctttttcaag
     1561 atatgttcat caattgttgc tgtatcttta agaactagtc tcagccgaga gatacagtta
     1621 tcgatttctt gaatattacc agcaccacca agtccttgca agattagttt gccaagttcg
     1681 ttttctgtca tttcttcgtc ctgatcttcc aacacatcat cgttggatcg accaggagtt
     1741 ttcacatcaa atcgctcaat gacatactta aatacaaagt aatatgcaac tgcataaaca
     1801 aggcccaatg ctacaactaa gtaccaacga gtataagtac cttgtaaaat tccgaagata
     1861 gccaaatcaa taaatccacc accggcattc ccaactgcga ctccgaatag attcattaac
     1921 ataaaggata aaccagccat gaatgcatgg aagacccata gaatcggtga gataaagata
     1981 aaggcgaatt caattggctc agtaatcccg gttacaaaac aagtagccgc tgctgcaatc
     2041 aacatccctt taatctttgg acgttgcgcc aaatatgaag ttttgtacat tgcatagcat
     2101 gctgccggca atccgaagac cataaatgga tgagtacctt gtgtcaagaa gcgagtagca
     2161 cttctcaaaa tatccatgtc ttttactggc aatgctaatt cagtattgaa gatggttaat
     2221 gctccagaaa ctgtttcacc attgatagtt gccgtaccgc cgattggtgt aaatcgaatc
     2281 aactggttta gtacatggtg aagacctgtt ggaatcagca ggcgctctga gaagccatat
     2341 aaaaaagttc caattacgcc tgacttacca atcaaaccgc ctaaagcatt gatagctgta
     2401 ttgacaaaag gccagatgaa aaatgaagcg attgagatga ttggaatagt taggatgatc
     2461 ataattggaa caaaacgatt gccactaaag aaactgaaag ctgctggtaa ttgttttgtg
     2521 tgatagcgat tgtggatcat ggctgtccat aatcctgcaa tgataccgcc gaaaacaccc
     2581 atacgatatg tgaatgtgcc aagtaaagtt tcatacatat tggaagtttc tactgctttg
     2641 atttgactca ttccgtcaga catcaatgtt tcaacagaag tgctttccgc tgtgatgcct
     2701 ctaagctgaa gcagataatt gataactaca tggaacagaa taaatgcaat aaccactgaa
     2761 aatgctgcaa tttccttatc ttctctagac atcccgattg ctactgaaat agcaaataga
     2821 ataggaagta gtccaaacat cgtaccaaca atagcctgta ctccaccaac aaatccttgg
     2881 atcacggttg cattagcgaa gctttctccc accaaagtag gattttgtaa aattgaaacg
     2941 attcctagta gtagtcccga tgcgacaata atagacaacg gacccattaa tgatttccct
     3001 aacttctgga tataagttct caatataaac accttcttct ttttagttga tataaacgtt
     3061 tacatccctt gtacaacgaa agtatagcac cctgaaaaag cgctaacaat acgataggtc
     3121 tgataaaaat caacattcag cacttggttg ttcattcaaa acgtgaatct tcaatcacag
     3181 aaaaatacgc gctatcaagc acgtatttta ggtgtaaata tatgcatcaa ctttattaat
     3241 caacgctact ttgtttgtaa tagttaatat acccttcata cagtaagcta agagcaagat
     3301 tcaaggtgca gaatgatgag ttgttcaact cattcacatc attaatgttg gtcacttgat
     3361 agtataggct atagtctgac agactggcta agttattttg accaaatcgt gtaactgaga
     3421 ccaactgtgt ccctttcata tggattcttc ttaaagtatc atcaatccga tcaacacgtc
     3481 cggacaatga tatgacaaat agtaaatcat tcgaggtcaa agcttttgaa atcaggtcta
     3541 attcattctt ttccggtaca acgataatgt agattcccaa gttccacatg cagcgtgcaa
     3601 actcattgag catcattctt tgtccatatc ctgttgcgta ggcaaatatc ttatctgcct
     3661 ttctaatcgt ttcatatata tcctcaacac ttttatcatc ttcaaacaat ttggttgtcg
     3721 cttgaacatc tccgagtaat acttccggta aaaacaaact acttttatcg actctggttt
     3781 tttgcatgcg tgcaaattcc tctttaaggt aatatcgaaa ttcgccatat cctttgaatc
     3841 cgagtttctt agttagtcta ataatcgttg agtcagacac atttgcttgt tttgctaatt
     3901 ccgatacagt ttgactgatg ctgatttcca tattttgagt gataaaactt aatacataat
     3961 catcattctc attcaattta ctgtgatgtt catttactaa ttgctccaat gtcataatca
     4021 gacctccaaa aataataaaa aagattgctc tctcatttta atacgaaagc acaatctttt
     4081 taagtactat taaattttga aaagaagttc tccttcagaa accttttttt cagagacttc
     4141 tagtttaata cccttatctt cgggaaatag aaccataaca acatctgaca gtttttccgc
     4201 cttgatttgg tcacaatcaa aaatcaccaa tgactcgccg gatgatactc gttgaccttc
     4261 ttcaacgagc acttgaaacc cgactccctt taactgaaca gtatcaattc caatatgaat
     4321 caataaatct ctaccttgat tactagtcaa tgtgagtgca tgctttgttg gaaatatcga
     4381 tttaatgaca ccgtcaatcg gtgctaccac cttagcgttt gttggttcaa cagcgaatcc
     4441 ttcgcccatc atcccttgcg aaaatacagg atcgcttact tggctaaggg gaatcagatt
     4501 tcctgatact ggagcaacta atttttcttt tcgactaaaa aatcccattg acttactcct
     4561 taatcttttg aaatgtccat cacaaggttt aaaagaacat ccgccccttt tttgacatcc
     4621 tcaaaatcag tccactcatc aggatgatgt gtaatgccac ctttggatgg aatataaatc
     4681 atatttgttg ggacaaagtc ggtcatcatc atcgcatcat gtccagtacc attgttaatg
     4741 acttgatagc tatagcccaa ctgatcgcaa atatgtttca tcttctcaac attaccttga
     4801 tccataacac ttggatttgt aggattactg tagttaattc gagccatttc ctccagctca
     4861 gttctaactc cagtttcccc ttgaatcact gataactcat tcaacaactt ttctttaatc
     4921 tcttgaggat tagttgtctt tgtatcaaag gttctgattt cgattaaagc ttcgacaaag
     4981 tcagggacaa attgatttga gttcggtttg attgtaagtt taccgacggt tccggcaatc
     5041 ccttcaccaa gattcttcat ccactgttca acaaaaagaa caaatttaga agcagcaact
     5101 aaagcatctt tccgtttatt cattggagca gtcgagtcct cagtttctcc ataaaaacgt
     5161 gctttaaaac ggccgatgcc aggaataaat tcaatcaatc cgatatcttt gccttcatct
     5221 tccaggactg gtccttgttc gatatgaagt tcaataaaat ttttgattgt ttttggatct
     5281 cgcttagcgg cctctaaatc tggttccact ccataagcac gaagagcttc caattttgtt
     5341 tgtccatggc gattttttac tgtttccaac tcactcatgg ttaatgttcc catcatagct
     5401 cgtgaatttg agacacccgt acttggccca aatgtctcgc cctcctctgc attcatcaga
     5461 attaggtcaa ttgggtaatc attttcaaaa ttattttctt ccagaatccg cattgtttcc
     5521 agtgcagtaa caacgccagc cactccgtca aatctgcctc cattaacaac tgaatcataa
     5581 tgagagccaa tcatcactga tggtgcatca gcctttttac cggctctacg accaaataat
     5641 gtagagaagc cgtcttccca aatctctaaa cccagttgtt ccatttgatt gatcaaataa
     5701 tttcttgctt cacgatcttc tttagagtaa gcagagcggg tcactccttc acctggagtt
     5761 gcattgtact cggctaaatt ttcgatatcc ttttttatac gctcaaaatt tgtcttcata
     5821 attaaatcct ccatttccta caagtgaatg ttagcaccca tagaaagcgg ctacaatata
     5881 atcaatgaag attgaatcga tattcgaaaa ctgaatattg cttcggttaa tgacttagaa
     5941 atcattatcg tagattccac cataagaact ttttctcgtc tcataacaca actcgagagt
     6001 ggattggttt aaaaaactct cgataaatgc gtccacttta ttgacacaga tccatggatc
     6061 gatcgcacct tgaaatttaa acccagaaat agtcccatca tcttctatac cgattaccag
     6121 cttacctcca tcggcatttg caaacgctac tatgtgtttt ataatatctc taggcttaat
     6181 ccgtgcactt tttctatcaa aatactgatc ttctttttca cttataaact tttgaatctc
     6241 ataatccatt taaaatttcc cttcttaaat ttttttatta tagagccgta tattcaattt
     6301 ccacaaaaaa ataatctaaa acaaaacaga caccttcatt ttgatatgtt ctcccacata
     6361 tgaatcggtc gctatacagc attacatata gtaattattc atactgcgaa ataaatagct
     6421 ccaaatcttt agctggcgta gttaagctta aattcgaaat gaaagtttca ttgactattc
     6481 cactttcatc aaatcccttc ttcaaccaat cagagttcca caacgtaaat cctttaggat
     6541 tagaaacttt cttacataat tttgcactat aggatttctc tcttgaatga attaaagaaa
     6601 gtctttgtaa ccaaatttca actatatccg tattaggagt attaacaaat tttcttaaga
     6661 ttttatccaa gtataagttg atttcttcta cagcaataaa ctcaaaaagc ttacctaaaa
     6721 tcacaataca agcctccaaa gttctaggat tcttttgcat tatattaaca agaatactaa
     6781 tcaattggta gatatcatct ggtcttttgt ttaaagtaga aactctatgt gtatagactt
     6841 cacttagcgc tttacttaaa ctcccgcaat ttggatattt gtctcctaaa agctttacct
     6901 gtagcaaatg cttttgaagg ctaagcttaa acttccactt attattactt ttctctgtaa
     6961 aacttgcatg tttaagatcc cagtaaattt tatcaggctt aattgcatct aaaattatat
     7021 tatccgataa gaatgtttta tttgaattta gcttcaaatt tagatccgat aatacttcag
     7081 ataacaatct gataatcact tcagcttgat cttttcgaac cgaaaaaatt ctgtaatcat
     7141 ctctgtaacg aagtattttg aactctttaa tctctgtttt ttctatcttg ttcagtaaca
     7201 ttttatcagc atacccaagt actatttcag ctacaaaatc catcaataca tttccttgag
     7261 gtataccgtt cgtttgtcca ttttgtaaat atcgtatttt agaatctatt aagtttccaa
     7321 cgccttgagt atcttttcta tgtgtcttag cccattcttt tgaatgtact gcccacggga
     7381 tagtatgtgt gtacattgag ctataacaat cggtaatatc tgaatgaatg cagtagtcat
     7441 actccaaagc ataactaatc tgtgcttgtt caaggttctc ccaccaattt aaaatagttt
     7501 ccgcagtatc gctctttgaa gaaatagatt ccacaggaag tgatatacat tcaattcgct
     7561 tgtcggcttg gaattcttca aacttatttt tgatgtgatt ccaattattt ttctgtgtaa
     7621 tcccgtttac taagtctaca tatactaatg gatgtaatat ttgaaggggt ctccaagcat
     7681 aattaccatc tttattagct aatattcgat gattaacatc aactgcctca gagagcgatc
     7741 tcttatgtct aactagttca gtaaatggtt tgttttttaa taaatccact gctttctcaa
     7801 tgcctactga taaatcaata tagttgggca acgatgtact aaaaaaactt gatgttttta
     7861 aaaagaattg ctttgcttct tgatgattca tctctaaaat actttttcta gtcctagtca
     7921 aattcaaaat ctcctagatc aattaagttg taatgtaaga ggatacccta aggttacaca
     7981 ccctatttac tattatacca gtattcattc gtgatacata agggtgtaac attaagaact
     8041 ctatctaaaa gactacaagt agtcaccttt aattgtgagt aattttcttt atccaatcct
     8101 tgtgcaacct tgtcgtcacc ccttgtatat ccccgtaccc cggatgtttc ggaatatcaa
     8161 aaatgtgctc aaattcctga ttttcatcag tctcccactc atgattcaac ggttcatgcc
     8221 actctataaa ccttgaccat aaaatgttct ttctataaaa ctcaatagga atttcaaaac
     8281 agtaaaaatt gtatttgcta aaatctgtaa aattatcttt acgagcatac tttaaatggc
     8341 aggtatctcg ccacaaccaa attaaatctc gatccaatgg taactgaatt cgtttcgtat
     8401 actcttcttc aagccaatga agtgcaccaa ctgcattcgg attatcgaaa aagacagata
     8461 attcagtata gcgtttcttt ggataaataa atccttctga atcaacagat tgcaaaaaat
     8521 ctactggttg aaccgtgtac actttcattt attcgccacc tttttttgac tacttatagt
     8581 catttaccaa tttgaaatct gcatactttt tcattcaatt gagcccagca ttaattgtac
     8641 acgagttttc ttcactaacg gatagtatca ggatatacta atactatcct gaatagcgaa
     8701 atgaggtagc caaaatgaga aaagtcgatg aatatatcaa aaagaaatcc caaaaaagta
     8761 aagtatggca aagaacattt gagttagagt ataagaaact ctcagatagc ctcaaaaagg
     8821 acacttataa tccaaatgaa caaagtaaag cagataacca ataaagatta ccctcccata
     8881 tttaacattg cttcacgaat cagtaatcaa tgactacaag tagtcattga agcaaatctt
     8941 cataagatac ctgtagaata tccctaatag ctctcaattg atccagctta atatgttgcc
     9001 ggccaccttc aatcttaacc aatgtttctc ttgtcatagc gattttactg agctggagtt
     9061 ctcgcaccag ttctgtctgt cccattccct tttctaatcg gattcgtctg atattgctac
     9121 cgattacatt ttctttatct attaactttt cttccttcaa aaatagcacg ccttttggac
     9181 taaaattagt cctttttctt attaatctta aagatttggt atgctacaat gggactagta
     9241 ttagtccata ttcaatttta aggaggaatt tttttgaaga aatttgcaat cattctaacc
     9301 tgtgctttac tcacgggagc tataagtggg tgcgccactt ctgacacaaa ttctacaagt
     9361 actgaaacaa gtacaacaac ctcagaaaag aattatccta tacatttttt tccagttcca
     9421 gatggatttg aagcttatta tcatgataaa aaactagatg ttccattgtc agaaagtaac
     9481 tctattgaaa aagttttgcc aattgaaatt gaaaaaaatc aagagaatat aaaactcaag
     9541 gccgagcttc ccatactgaa cagcacagaa attgattggc acgagaatat taaagtaact
     9601 attgaaaaag gagcgttagc acaactttat actgaaaaag ttgaacaatt ttttgagtct
     9661 ggtgccaata aagacttttc aaaaataagc gattattcgg ataacttttt taatgagacc
     9721 caagatgaaa tcacgaactc aacttttatg gctgaagata tgtacaatat gacgttaaaa
     9781 aaaataaaag cttacgacca tccattaaat ctgacactaa ataatggatt actctctttt
     9841 gtattagcag gttacgttga ctatgaatat tcaccagatt atttggagac acctcttcaa
     9901 cacgagaaca atgatattaa ttttagattt gtctacgagt caaaaagtaa aacatggtta
     9961 gttgatcaat taagcactat aaacacaagc ttccatgaat tacctaaacc tgaaaatgaa
    10021 gctgatatgg tcattaaaat cttctaaaaa agaagcgttg aaggagaaat attcctttca
    10081 acgctctttt ttatatgctt agaaacataa ataaattgta tactttcttt ttagccccta
    10141 ttacttttca cgaaaaatat cttttttcga cactagcgtt tctctactta taatattgta
    10201 cacagttttt gtattattga aaaattactc attatttagc attaatccac atcgttttat
    10261 caatcgacct tcatactaaa ctactttaaa gtcatcttcc ttctcatttt tcattaaatc
    10321 agatacataa gcccatgtac ccgttcgttg taaataaagt tcttcttgta gcagctccca
    10381 ctttctctta gaaatcataa tgacgccatc gttcaatcca tttttagaat ccctagtaat
    10441 ttctatttca gaatttgaat gaactacttc ttgcaacact ttacctatat tttttttgaa
    10501 gtttgttgga gtaatagtat ccaaaatgaa cacctctctt ctctatacct ctattatgaa
    10561 gcgcccatca aataaagcaa gcgttgagga ataattcttt caacgcttgc tttattaagc
    10621 tttcattttg tagtttgact aataccaata atctgatctc taaacaatcg aaaaacttct
    10681 tgtggttgat aataatctgt atctatttca aatgtttctg agaacaattc attgaatttt
    10741 tctactgaaa tgatgatttt accattctcc tctcgactgt tttctattat ttctgcttct
    10801 aattcaaata aatctagtga cgaactaaaa tcataaatcc tgtcatattg aacaaaaaaa
    10861 tcaagaaaaa gttgctcaaa ctcttcctta tttatcacta gtgtttcaag cggaacaact
    10921 ttattttcat cagccattct tccaatttcc tcactttcat tttcaaaaaa tcgtaaaatt
    10981 gatatagatt ctttgtctct aatcactcgt gatattatct tgacagtcct acatctaatt
    11041 caattaaagt ataagccact ttcaaataaa agattacaaa gtaaatggca agccaacaaa
    11101 ataaccatct gcttcacgta ttacgatttg ataattctgg atttcgctcg gaagttcata
    11161 ataataatta acagtttctg ctttgccact ttcaatatat tctggattat tcaatatcat
    11221 agcatactca taaagttctt caccattttt att
//
LOCUS       DACEUZ010000091        11068 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.091, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000091 DACEUZ010000000
VERSION     DACEUZ010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11068
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.091"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            4..2376
                     /locus_tag="IXG44_001955"
     CDS             4..2376
                     /locus_tag="IXG44_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1658668.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNDQIEKMFEHQRKTNGQ
                     PMNDKRNGHSWFRQQERIENKVQSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     TPTDSVKEKETDHFYRVEFNENDPAKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNHPIYLELARLCEQVPEKEQKQTVKNEPISKV
                     QNEIKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DQTGHTELQVGIQAVAKNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     ATWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQD
                     EKNVVETAEQKNIVLVGSLWIDKKDGKVLEEDTGRTLHLKDAAFIEDTQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            2437..3897
                     /locus_tag="IXG44_001956"
     CDS             2437..3897
                     /locus_tag="IXG44_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HAQ1658669.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDSKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPDWLIDYAEKEGLIAELKPKHERQNF
                     LVQDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKVI
                     YEEVAKEMKVEPEAIMAIHKTENNLQLTNQLVSAHKVNASFGQQLSVNDSIEAINLKD
                     ICREVAKELKGCERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIHQQEIRKQKRAMLFQQGKQQERE"
     gene            3908..5917
                     /gene="topB"
                     /locus_tag="IXG44_001957"
                     /pseudo
     CDS             3908..5917
                     /gene="topB"
                     /locus_tag="IXG44_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303486.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
     gene            6263..6859
                     /locus_tag="IXG44_001958"
     CDS             6263..6859
                     /locus_tag="IXG44_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ1658670.1"
                     /translation="MTKAMVYKLLEEFNGTLALKDAKKAGISPVTIKRMVDRGELDRE
                     YPGFFTLPGQFPDELFMAQKKYERGIISHITALDLYDLTDMIPRQIDLTVPYGYHVSE
                     KGLKEFAVELHYTKAEWYELGKIEIKSRYGNPIIAYDPERTLCDIWNPWYTVEDEIKV
                     KAIKNYMESDRKNLRKLNEYRRILPTDKTMRSYIMALN"
     gene            6872..7771
                     /locus_tag="IXG44_001959"
     CDS             6872..7771
                     /locus_tag="IXG44_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAQ1658671.1"
                     /translation="MTPTQLKAKVKNISREKEVDPQLVLRHFMMEKFLEKISESPYRE
                     NFVLKGGFLIGSKYGIENRTTKDIDTTLREMKVTKETLTTVLNEIFSTPTKEDIQFEI
                     QGMKETREADYYPGFSLRVLAHLENMRPDFKVDVTTGDSIYPATITHSHKLMFEDRTI
                     PLESYPTEQIIAEKLSATFDFLTDNSRGKDFYDLYTIPKMEKIDEKTLKDSVRNTFTR
                     RGKTDPLKIYYEKDMDVLKNSHELKDTWKKYQKSNPYAASITYDETMDSISNLMEKVI
                     HVEEKEQKLAYQRHQQMNREMER"
     gene            7774..8258
                     /gene="ssb"
                     /locus_tag="IXG44_001960"
                     /pseudo
     CDS             7774..8258
                     /gene="ssb"
                     /locus_tag="IXG44_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
     gene            8280..8537
                     /locus_tag="IXG44_001961"
     CDS             8280..8537
                     /locus_tag="IXG44_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1658672.1"
                     /translation="MAKKISLTMDLLIPVIAFIDGISLGVTENPLVNRIAKGSLFLVF
                     VYLVCSYLLKRAGKDNSFWRLTKVTVAATAVILTAILPLYM"
     gene            complement(8696..8830)
                     /locus_tag="IXG44_001962"
     CDS             complement(8696..8830)
                     /locus_tag="IXG44_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HAQ1658673.1"
                     /translation="MLKSIKGVSLLSAYETIQTILGFGMFTIALISLIVNMLKNDKKK
                     "
     gene            8973..9233
                     /locus_tag="IXG44_001963"
     CDS             8973..9233
                     /locus_tag="IXG44_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1658674.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIESMQIRNPDHFTRSMGI"
     gene            9682..9888
                     /locus_tag="IXG44_001964"
     CDS             9682..9888
                     /locus_tag="IXG44_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HAQ1658675.1"
                     /translation="MRKKFDYLGVPFSELFPNYHAPHTVECDCGERAKCIKSYCLYQC
                     PTCGKKYTLSYGDYILIEEKGKKR"
     gene            9888..10139
                     /locus_tag="IXG44_001965"
     CDS             9888..10139
                     /locus_tag="IXG44_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1658676.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGCELHYQENLETYENEYWLTKLEN"
     gene            10155..10592
                     /locus_tag="IXG44_001966"
     CDS             10155..10592
                     /locus_tag="IXG44_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1658677.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYNETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNAKWFFQALLCFEIGVKPSTIKPSEYQALE
                     LTYAKFIETKKAKTVSSEWLDYFENINKYGAYYTMKKEDNENE"
BASE COUNT         4087 a         1887 c         2324 g         2770 t
ORIGIN      
        1 gacatggcag aagaaaaaga accactagac aatagtcgtt tgggaaaatc caaaagaaaa
       61 ttagtacgat tgcaaaatga attaaatgat caaatcgaaa agatgtttga acaccaaaga
      121 aagaccaacg gtcaaccaat gaacgataag agaaacgggc attcttggtt taggcaacaa
      181 gaacgaatcg aaaataaagt ccaaagtctg agagaagaga tcaagcaaca agaaaaacag
      241 gtagaaaaac ttgaaagaca agaagagcta aaggaaatgg gttataacaa gtacggtggc
      301 ctggatatga cgatcgaaaa tattccaaga atcaaagaag aaatcgaacg ctttgaaaaa
      361 ggtgaatcga ctttttcagc tgccacgatc cgaaaatacc aacgaaaatt ggaaacatta
      421 gaacagctga aagaaaaatc agagaaagga aaagaaaata ttcttcctga agtacaagcg
      481 atcattgaca gcggacgagt gacgcaatgg aaaaagaatc caactatcta ttttctgaaa
      541 ggctatcgaa aagtcgcttt agaactcaat gaaaacgggg gatttgagga atctaaaaaa
      601 taccaagcca cgtcggaaga agaaaaggca gctgttcgtg aattacttga agaagctaca
      661 cctactgatt ctgtgaagga gaaagaaacc gatcatttct atcgagtaga attcaacgaa
      721 aatgatccag caaaagggtt gaaaagttat ggtggggaga tcgtaacact agaattgatc
      781 gatcaactca aaacactgga taaccaatac gccgcagaag caggctatta taaattctat
      841 tttgaagaag tgaagaatgg agaagtagta gatcagatac gaatggatct aggggatggc
      901 gtcaatagta atcaccccat ctatttagag ttagctagat tgtgcgaaca agtacctgag
      961 aaggaacaaa aacaaacagt caaaaatgaa cctatttcaa aagtacaaaa tgagattaaa
     1021 gagacaaaaa caaaacaaaa gggttatcaa aaacgaacgg cagaagagat taagcaagaa
     1081 gtcaaatcac tttcccaaca ggcgcttgat gcggtgaaaa agcatactca ttcacctgaa
     1141 gacgtcaaag aacttttgaa ttttatgtct cgttttccag aacgatcctt tagaaatcaa
     1201 ttattgatcg aggaacaatt tccaggcgca acggcttgtt taggtcgtgc cgcgttgaaa
     1261 aaagaaggaa tctatatcaa aaaaggggaa caagggaaca agatctttgt cagaaaaacg
     1321 gtcaaaggat tttatgagaa aggacgtggc tttgttcgag aaacagaggc tacaccagaa
     1381 gataaaaaac gaatcgaaag tggtcaaatc gaagtgatca aaaaacctta ctacacgatt
     1441 gaaaaagtgt ttgatatcac acaaacacaa ttgaagcctg aagactatcc gaaaatcttc
     1501 ccgaaccgcg tctttgattt tcaattggat caaacagggc atacagaact tcaagttggg
     1561 attcaagcgg tagccaaaaa tattggaatt caaattcgag atatgacaga aagtgaagtc
     1621 tatcaaagag aattaggaca agcacgtggg gcgtacgttc gtaacgaatt tactggcgaa
     1681 gaagaaatcg tcatgaatac tcgaaatact cctacgcagc atttagccac aagtattcac
     1741 gaattagccc atgctagaat gcacaaattt tcagaattag atactgcaac aaaagaacta
     1801 caggcagaaa tgacctccta tattgtgtgc aagcattttg ggatggacac ctcggagaaa
     1861 gctattcctt atattgcgac atggacgaag aatggtcaga cgttggatga taaggaagct
     1921 gcggaacgtg ggaaaatcat gaatgatgtc agtcgtgtcg caaatgaatt cattcaaacc
     1981 atttctaatg aaatcaatca atacagagaa agagaaccag agatccagcc agcagaagtg
     2041 aagcaggatg agaaaaatgt tgtagaaaca gcagaacaaa agaatattgt gctcgttggc
     2101 tcactttgga tcgacaagaa agacgggaaa gtcctggaag aagacacggg ccgaacgtta
     2161 catttgaagg atgcggcgtt tattgaagat acgcagcata atcaagtgat tttaaaagga
     2221 aaaagttatg gaaaagagat tcaaacggcg ttaccttcag aagaatttgc gctaggaaat
     2281 ccattccgat tttcacttga aacagtgtca gaagtggcca aacgaaaaaa tgaaatctat
     2341 tatgaacaac aattgtcaca aaaagaacgc gtataaaaca agtaaaaaag actatctgaa
     2401 aaacggatag tcttttttct tttgggaggt ttgaaaatga ccgagaaaaa aatgagtctt
     2461 attgatcgat gtaaacagat cgatatagtg gattttgctc gaaacaatgg aatggctgta
     2521 gtcaataaag gacgagacta tcgcttagaa gaccatgatt catttgtttt tgatcgaaga
     2581 aaacaacgtt tttattggaa cagtcaaaat attagtggcg acattattga attagctaaa
     2641 ctttttttca tagacaaaga gattcaagac tctaagcaac aatttaaagc agccacagat
     2701 tttattctaa agaatgaaga caaaacagaa cgagtagaaa atcttcattt tgagacagaa
     2761 aagtacaaag atcaccccgt tgattatcaa ccactcacag aaaaaggaag gaattatttg
     2821 aaggaagaac gaaaactgcc cgattggttg attgattatg cagaaaaaga aggattgatc
     2881 gccgaactaa agccaaaaca tgaacggcaa aatttcttag ttcaagacga tcgtttggat
     2941 catgcggtcg cctttttatg gaaagatcca cagacaaaag agacagtagg ggcgagttat
     3001 caaggaacat tcattgatta tgaacgtttt ggtgagagag gcacatataa gcatatagat
     3061 aaaaattcga cagcgaatca tggatttaac ctaaagatcg gtgaccctaa acagttaaaa
     3121 ttttttgaga gtagtattga tttacttagc tatgctgcac tgaatcgtga tcagttaaac
     3181 gatacgtggt tagtctctat ggaagggtta aaacaccatg tgatcagtca ttattttggt
     3241 gaggcagtat ctgaattgag aaagaaacaa gcctttcctc aatccattga gatttgtgtg
     3301 gataatgacc gtgcaggaca tattttctac gaaaaagaac aattaatggg cgcagttgat
     3361 ccatttacga atcagaaagt acgttgtgaa cgaggaatcg ctaatgattg gcaagtgcca
     3421 aaagaataca aagtcattta tgaagaagtt gcaaaagaaa tgaaagttga acctgaagca
     3481 attatggcca tacacaagac agaaaacaat ctgcaattaa cgaaccaact ggtttccgct
     3541 cacaaagtaa atgcgtcttt tggtcagcag ctttctgtta acgattcaat agaagctatt
     3601 aatttgaaag atatttgtag agaagtcgca aaagaattaa aaggttgtga acgcgtggat
     3661 ggtacgtacg attttgatcg tttttatcaa gaaaaagggg atattaatgc tcagattctt
     3721 ttctcttata aagcagaaca atactacaaa ggctataaaa atcatgaaca tgaatttgta
     3781 cctgaagtaa aaaaggattg gaatgatcaa ttgaagcatg aaatccacca acaagagatc
     3841 agaaagcaaa aacgtgcgat gttgtttcaa caaggcaaac aacaagaaag ggagtgaaat
     3901 cgtcatgatg aaaaccgtta ttttagcaga aaagccgtca caagcgaaag cttatgccga
     3961 tagcttctct aaagccaccc gaaaagacgg gtattttgaa atccaagata gattgtttcc
     4021 tggtgaaact gtgatcacgt atggttttgg tcatttagtc gagttagatt cgcccgatat
     4081 gtacgatgaa aattggaaac aatggtcatt agagcatttg cctattttcc ctacacatta
     4141 tcactatcat gttcctaaag ataagaaaaa gcaattcaag gtggtgaaac agcaacttca
     4201 atcggccgac accattatta ttgcgacaga tagcgatcgt gaaggggaac tgatagcttg
     4261 gacgatcatt caacaagcag gcgccgatca tggaaagata tttaaaagac tttggatcaa
     4321 ctccttagaa aaagaagcca tttatcagtg attccagcaa ttacgagacg cagaagagac
     4381 ctatcccaaa tttgaagaag cacaagcacg ccagatcgct gattggttga tcggcatgaa
     4441 cggcagccca ctgtatagtt tactcttgca gcaaaaaggc ataccaggaa gtttttcctt
     4501 aggcagagtc caaaccccaa cgctttacat gatttatcag ctgcaggaaa agattagaaa
     4561 cttccaaaaa gaaccatatt ttgaaggaaa agcccaagta atcgctcaaa atggcgcatt
     4621 tgatgcgaag cttgatccaa atgaaaccca agcgacacaa gaagcatttg aagcctattt
     4681 aaaagaaaaa ggtgttcaat taggaaaaca gccaggtacg atccttcaag tcgaaacaga
     4741 gaaaaaaagt gcggctagcc cacgtctttt ttctttatcg agccttcagt ctaaaatgaa
     4801 tcaactcatg aaagctagtg ccaaagatac tttggaagcc atgcaagggt tgtatgaagg
     4861 aaaatatcta agctatccgc gaacggatac cccttatatt acggaagggg aatatgcgta
     4921 tctgttggat cacttagacg aatacaagca ttttttaaaa gccgaagcga ttccgactcc
     4981 tattcataca ccgaatagtc ggtatgtgaa taataagaaa gtccaggaac attacgcgat
     5041 cattcctaca aaaacggtta tgacagcagc ggcttttgaa caactaagtc cactgcagca
     5101 ggctatttat gaacaagtgt taaaaacaac ggtggccatg tttgcggaaa agtataccta
     5161 tgaagaaaca acgatcttga cgcaagttca gcagcttcag ttaaaggcaa tcgaaaaggt
     5221 gccgctagat ctaggttgga agaagttatt tggaaaagag agcgacggaa aagagaagga
     5281 agaagagccg ctacttccta aagtgacaaa aggagaaatg gtgacggtgg atctacaagt
     5341 tcttgaaaaa gaaaccaagc caccacaacc ttataccgaa ggtacgttga tcactgcaat
     5401 gaaaacagct gggaaaaccg tggatagtga agaagcgcaa tccatattga aagaagtgga
     5461 aggaatcggt acagaagcga cacgggcgaa tattattgaa accttgaaac aaaaggaata
     5521 tatcaaggta gagaaaaata agctcgtggt gacgaataaa gggattttgc tttgccaagc
     5581 cgtggaaaaa gagccgctgc ttacgagcgc cgaaatgacc gccaaatggg aaagctactt
     5641 gttaaagatc ggtgaacgaa aaggaacaca aaccactttt ctagccaata tccaaaaatt
     5701 tgtctctcac ttattggaag tcgtaccagg gcaaatccaa tccactgatt ttggatcgac
     5761 gttacaagaa gtgaaagcag ccagtgaaaa gcaagaagcc gcgcgtcatt taggaatttg
     5821 tccaaaatgc caggaacaag aagtcttact ctatcaaaag gtggcagctt gtacgtctga
     5881 agcctgtgat tttaaaaggt cagaagggtt catttgaagc aacgattgtt ttaaaagaag
     5941 acttcactac aggctttgaa ttttctgaaa agaaaaaaac gaacttcaag aaaagaacac
     6001 gccgcactgc aaaataatgg agggttaatc ttatgcgtaa taccacacac aaacaacaag
     6061 aaatcgaaaa tcaaaaagcc aaaattcgtt cagaacagga aaaaatgcgt gccagcaaac
     6121 gaagactgca gcaattgaag aaccagcaga ggattcaaaa agaaatcgaa ttagatcgat
     6181 aaaatcttcc taactattgt atacgtataa acttatgtta tactgttttt ataagtttat
     6241 acgtatacaa aggagggaga aaatgacaaa agcaatggta tacaagctgc tagaagaatt
     6301 taatggaact ttggctttga aggatgccaa aaaagcgggc atttctcctg taacgatcaa
     6361 acgtatggta gatcgagggg agctggatcg agagtatcca ggttttttca ccttacctgg
     6421 tcaatttcct gatgaattgt ttatggcgca aaaaaaatat gaacgcggca ttatttcaca
     6481 tattaccgcg ttagatttgt atgatttaac agatatgatc cctagacaga tcgatctgac
     6541 tgtaccttac ggttatcacg tttctgaaaa aggattgaag gaattcgcag ttgagctaca
     6601 ctatacaaaa gcggaatggt atgaattagg taagattgaa ataaaaagtc gctatgggaa
     6661 tcctattata gcttatgatc cagaaagaac tttatgtgac atttggaatc cctggtatac
     6721 cgttgaggat gaaatcaaag taaaagcaat caaaaactat atggagtcgg acagaaaaaa
     6781 tcttagaaaa ttgaatgagt atcgacgaat tttaccaaca gacaaaacga tgcgttctta
     6841 tattatggct ttaaattaaa ggagaagata catgacacct acgcagttaa aagcaaaagt
     6901 gaagaatatt tctagagaaa aagaagttga tccgcagctt gttttaagac attttatgat
     6961 ggaaaaattc ttggaaaaaa tctcagagtc tccttatcgt gaaaattttg tgttaaaagg
     7021 cggcttcttg attggctcaa agtatggcat tgaaaatcgc acaaccaaag atattgatac
     7081 gactttacgg gaaatgaaag taaccaaaga aacattaaca acggttctaa acgaaatttt
     7141 ttcaacgccg acgaaagaag acattcaatt tgagattcaa ggaatgaaag aaacgagaga
     7201 agccgattac tatccaggat ttagcttacg tgtgctggca catttggaaa acatgcggcc
     7261 agattttaag gtagatgtga ctacaggtga ctcgatctat cctgcaacga tcactcacag
     7321 tcataaattg atgtttgagg atcgaacgat tcctttggaa tcctatccta cagaacaaat
     7381 tattgctgaa aaattaagtg caacctttga ttttttaaca gataattcga gaggaaaaga
     7441 tttttatgat ctctatacta ttcctaaaat ggagaagatc gatgagaaga ccttgaaaga
     7501 tagtgtgaga aatacattta cacgtcgagg aaaaactgat ccgttaaaga tatactatga
     7561 aaaggatatg gatgtactga aaaatagtca cgagctaaaa gatacctgga agaaatatca
     7621 aaaaagcaat ccttatgctg catcgatcac gtacgatgag acaatggatt cgattagtaa
     7681 tttgatggaa aaagtgattc acgtcgaaga aaaagagcaa aaactagctt atcaaagaca
     7741 ccaacagatg aatagagaaa tggagagata aagatgatta ataatgtgac attagtagga
     7801 cgcttaacca aagatcctga tttacgttat accgcaagtg gtactgcagt agcgactttt
     7861 accctagccg tcaaccgaaa tttcacgaat caaaacggca atcgagaagc cgattttatt
     7921 aactgcgtga tttggcgcaa accagcagaa acaatggcga cattagctaa aaaaggtagc
     7981 ttgattggtg tagttgggcg gattcaaacg cggacttacg acaaccagca aggacaacgt
     8041 gtatatgtga ctgaagttgt agcagataac tttcaactat tagaaaataa agcagcgaca
     8101 gagagccgtg cgcacgctga tcaatcgtcc accagtccaa gcaccgccac ctttgaacaa
     8161 cggatacagc gacgcctaac aacaatggat tgaacgcttc acagaatcca tttggtggac
     8221 aatcaatcga tattagcgat gatgatctac cgttttagtt tgagaaagaa ggagtaagca
     8281 tggcaaaaaa aattagtctg acgatggatc tattgatacc cgtaattgca tttatagacg
     8341 ggatttcttt gggcgtcact gaaaatcctt tggtaaatag aattgcgaaa ggatcactat
     8401 ttcttgtatt cgtgtatctt gtctgttctt atcttttaaa aagagcagga aaggataatt
     8461 ccttttggcg attgactaaa gtcacagtag cagcgacagc tgttatactt acggctattc
     8521 taccgcttta tatgtagagt taacgggaaa tcgttgaact tcttgcgaaa tattgctata
     8581 atgaacatga aaaagaggag ttgcgctaac aacccctcat taacagcacc gattaaagaa
     8641 cggtggcaga atattagtta tttggaaata accgctgacc tgctaaagta gacggttatt
     8701 tctttttgtc atttttaagc atatttacaa tcaaactaat caaagcaatg gtaaacatac
     8761 caaaaccaag aatggtttga attgtctcat atgcggacaa aaggcttact ccttttatag
     8821 attttagcac ttacattcat aagcaccacc acctttccag aagtcgccac cgtcttcaaa
     8881 ctttactgtc ttgactatta tagcatatct aaaatcgaat aaaaattctc aattttaact
     8941 gtttttgcta taattttagc gaggtgatcg tgatgaacaa atatgttggt ttgcttgata
     9001 aaatccgagt aataaaaaca caaccattat tggttcgctt tactttacaa acaattcatg
     9061 aatcgattaa ttgtgtagtt gcagatattg aaattatcga taaattgtta attatggatg
     9121 atggaaaata caatattgcc gtcacaggtc atttcaataa acggaatcaa ttagtcattg
     9181 aatcgatgca aattagaaac cctgatcatt ttacgcgatc aatgggcata taaataaaag
     9241 aagagctgca atcctaatgt aggactgcag ctctttttgt tttacataga aacaggaaga
     9301 aagggaaagg cctgtttagg aaggcacaag tgccttctca attgaaaaac ctttctctca
     9361 ctccaaccgc caccgccgtt cgagaagaaa ggaagtgaac gttgcacgtt cactaagata
     9421 aggggtcttg ctttgtaaga caaaatagga aaagcttcct ctcaccaaaa ggttcgttcc
     9481 tgttcgctat ctctttattg atttgccaga ccggcaacaa gttgcctttt atcaccaaaa
     9541 agcgacaagg acaagggtaa atggcacgct gcgcgccctt gtccttgtcg cttttcgttg
     9601 attcattctg gccaacaaat caataaagaa agaactaacg aaaggaagta aaacctttcc
     9661 taagaggagg cagaatcaat catgcgaaag aaatttgatt atttgggtgt tcccttcagc
     9721 gaattattcc caaactatca tgcaccacat actgtcgaat gcgattgtgg agaaagagcg
     9781 aaatgtatca aaagctattg tttgtatcaa tgtccaacat gcgggaaaaa atataccttg
     9841 tcttatggcg attatatttt gattgaggag aaaggaaaga aacgctgatg aaaacacaac
     9901 acaaattatt gactgtaaaa gaagtcgttc aaattttggt ggacaaagaa gtcatttctg
     9961 aaaaagtagc agaagcttta cttattccgc ggtactggac gatagaaacc cctttagtag
    10021 aggatgcacc gactatgcta tttgaagacc tagtgaagat tgcaggatgt gaactgcatt
    10081 atcaagaaaa cttggaaact tatgaaaacg aatattggtt aaccaaatta gagaactaaa
    10141 aaggagaaaa aaatatggct caaaattact atgacgaatt tgtcaaatta cctttggata
    10201 aaatggcaca aaaaatggag gatatgacgt tcctatacaa cgaaacacgt gttccaaaaa
    10261 aacattataa agaaaaattg tcggtagctg tggaagaaat gattgaatca ggtgtcgaaa
    10321 tgaatcttat tgcgacgtac tatcgaacac tggaagaact gaaaaagcaa aatgcaaaat
    10381 ggtttttcca agccttacta tgctttgaaa taggtgtcaa accaagcact attaaacctt
    10441 ctgaatacca ggctttagaa ctaacgtatg cgaaatttat cgaaacgaaa aaagccaaga
    10501 ctgtttcaag tgaatggtta gattattttg aaaatatcaa taagtatggc gcatattaca
    10561 ccatgaaaaa ggaagacaac gaaaatgaat aaggtgattt atcaactaac aacggattcc
    10621 atatcaacga tttgaaagaa aaaaaggtca ataatcattt ataccgaaaa gtgagaatgt
    10681 gtggaataag gacaaaaaaa gcctagtaac taagggaatg gttcttattt cgcaccaacc
    10741 gcccccgccg cttaggtggt gcgaaataaa cgggcaaagc ccgataactt aagagaaaga
    10801 aggtgatcca tttgaaaaag attattccct tattgttagt actgtttgtt ggcgaaatac
    10861 ttatgcgaga tagtgcagct gtacagttaa acctcgctgt atttttggta atactggtgt
    10921 tttgtttgat cttttggcgg tttgtattag tcctcgtgag tctactattc aagaagattg
    10981 cagccatatt tttcatgggg ttatttcgat gaaaggagag agtaccaatg ccaattgcaa
    11041 taggaaataa acggttaccc gtgacatt
//
LOCUS       DACEUZ010000092        11028 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.092, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000092 DACEUZ010000000
VERSION     DACEUZ010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11028
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.092"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            <1..997
                     /locus_tag="IXG44_001967"
     CDS             <1..997
                     /locus_tag="IXG44_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ1658678.1"
                     /translation="TNRYHLPFSRKLTNKINIKKIFSPSEPVWKTFEDIEYTDQLSDL
                     WDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAEELFALAKEKGLF
                     IQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENTHEFSTANSYLYG
                     HACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMKISIKSSYFRIKE
                     RPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGVLTYIDEQGSYHE
                     EKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIKSIQESEGKI"
     gene            1086..1391
                     /locus_tag="IXG44_001968"
     CDS             1086..1391
                     /locus_tag="IXG44_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HAQ1658679.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            1570..3585
                     /locus_tag="IXG44_001969"
     CDS             1570..3585
                     /locus_tag="IXG44_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ1658680.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            3587..3871
                     /locus_tag="IXG44_001970"
     CDS             3587..3871
                     /locus_tag="IXG44_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1658681.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            3897..5270
                     /locus_tag="IXG44_001971"
     CDS             3897..5270
                     /locus_tag="IXG44_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAQ1658682.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(5353..7368)
                     /locus_tag="IXG44_001972"
     CDS             complement(5353..7368)
                     /locus_tag="IXG44_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HAQ1658683.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(7390..8604)
                     /locus_tag="IXG44_001973"
     CDS             complement(7390..8604)
                     /locus_tag="IXG44_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1658684.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            8825..9769
                     /locus_tag="IXG44_001974"
     CDS             8825..9769
                     /locus_tag="IXG44_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1658685.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            10065..11009
                     /locus_tag="IXG44_001975"
     CDS             10065..11009
                     /locus_tag="IXG44_001975"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAQ1658686.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
BASE COUNT         3626 a         1975 c         2120 g         3307 t
ORIGIN      
        1 tacaaatcgc tatcatttgc ctttttcaag aaaacttacg aacaaaatca atatcaaaaa
       61 aattttttcc ccatctgaac cagtgtggaa aacattcgaa gacatcgaat atactgatca
      121 gctgtctgat ctttgggatg atcctgagat ccaattagtt gtcatcacta cacctagtca
      181 atttcattat gaatatggaa aaatggcact cgaacatggt aagaatgtac tagtagagaa
      241 accttttgca gaaacgtcaa aggaagcaga agagttattt gcattagcaa aagaaaaagg
      301 tctatttatc caatgttacc aaaacagacg gttcgattct gacttcttga cagtccaaaa
      361 agtaatcgaa agcggaaaat taggggatat tttagaagta gaaatgcatt atgattattt
      421 cagaccggaa gtaccagaaa atacgcatga attttctact gcgaacagct atttatatgg
      481 acatgcttgt catacagtag atcaagttct ttcttatttc ggcgaaccag atgatatcag
      541 ctatgatgta cgtcaacttt tagggaaagg acgaatgaat gattacttcg atctggattt
      601 tttctatgga aatatgaaaa tttcgataaa atccagttat ttccgcatca aagaacgtcc
      661 aagtttcgtc gtttacggaa aaaaagggat gttcgtgaaa caaacaaaag atcgacaaga
      721 agaacatctg aaaatgtttt acatgcctga tcatgaagac tttggtatcg atttgcccga
      781 acattatggt gtattgacct atatagatga acaagggagt tatcatgaag aaaaagtaat
      841 ttccgaggtt ggtgattaca gccgtgtcta cgaagggatt tacgagacat tagttaatgg
      901 cgcagaaaaa gtcatcaaag atgaagaaac aatcttacaa atgaaaattt tggaagaagg
      961 aatcaaaagc attcaagaat cagaaggaaa aatatgacat atttgtaaga atcgggtttg
     1021 ttttttgacg cataaactgt cttattgtac gatgaaaaag tatagaacta ctaggaggaa
     1081 ccaacatgac aaattatgac ggtgaaaaac acaaacgatt aagaggttca atggccccta
     1141 aaacagaaga agaagtggct ttagcaggaa ctaattctgg tgaaacagaa gacaaagaag
     1201 aatcattagc tggcacaaac tctggtgaaa cagaaaataa agaaacagct ttatctggtt
     1261 caaattctgt agaagaggaa acaaagccta atgagcccaa tcactttgaa aaagatatcg
     1321 aaaaagcggt tgaagatgtc aaagaaaaat ttgaagaact gacctcaaaa aataaacatg
     1381 atcatcattg atcggacgaa atcaattcat aaaagcagga gacatttggt cacctgcttt
     1441 ttttctgtat ccattcgata gaaatcgttc ggtacagtcg taaaaaaaat ccaactgtaa
     1501 gttgtaccaa acacccctcg atttaaaagc gctaccatac tagaatataa gtaggaggtg
     1561 aacagcgctg tgaactacaa aattagttat ctcctgaaaa caccaagaga aatcagtcta
     1621 gaaaaagcag cagagatcat cgaagtaaat acaagcgagg cgatcaaaca attaactcct
     1681 tttgccgaat atgtggatga tacccgtatc cgtataaaga aattccgagc aatcgactgg
     1741 gcagaaaaat ttttggatat tactcgattt gaagctgtgt ataccgaaca agagcgaaga
     1801 gatcttcttt atttgatggt ttattcgact gcaacgtctg tttctgtttt tcattttcaa
     1861 gattttcttc aggtttctaa agggacagtc ctgtcagata tcaaaaagat aagaaatgaa
     1921 ttagaagtgg aagattgtca gctgtcgtat actaggaaaa ctggttttag gctgctaggg
     1981 tcagaagcaa tattaagaag agaagcaaaa aatcgcactg ctcgtttgat gcagtcactt
     2041 gctggaagat ttgggctcac gtatttaacc gcaacccttt ccattgatta ttacgccaaa
     2101 gttagagatt tgctagataa aacgatttca ctatcagatc ttcgattcgc accaaatcga
     2161 atggatgaat tagcttatta tttgagtttg acaaccattc gaattggaaa aacatccatg
     2221 gaactggtaa aagatgctga actgttggct tctcttgcag tcactgacat tagtcgacaa
     2281 gtattgactg gccttggcta ttccgaacat caaaattcgg agattatatt tttctctctt
     2341 tgcttagcat cgatcgttga aggagatgtc caggagcctg cacttgattt tttgctggaa
     2401 tgttcttcag aagtcatcca tcgcatggaa ttcctgatgg ccgtagaatt tgcttcgttt
     2461 cgtgaattat tgatgaatgt cttttatcac ttggtccctg cctattttcg tatttcctat
     2521 tctttttatt tacctaatgt gatgatcgat caaattaagc atcaatatgc ttccatctac
     2581 gaaatgacaa gaaaagcatt acttccgtta gaaagacgaa tagggaagag catccctgaa
     2641 gaagagattg gttttttcac tatactattt ggcggagaga tcagaaaagc ggacgcagaa
     2701 gaaagaaata gaaaaataag agccgtgatc gtctgtccaa gtggcattag ctcttcattg
     2761 atcttaaaaa gtgaacttca gcaattattc ccgatgatct tttttacaga gactaattca
     2821 gctgaccgct tacacgaggt tgacgaaact tcttacgata ttattttttc tactgtacct
     2881 ctcacactta ctgataagaa aaagtctctc tatgttctgc atccaatcat gactgacttg
     2941 gaaaaaaatc agctgatcaa tcaagtacaa agagattggc tgataccagg attttcaatg
     3001 ccttctgccc aagaattgtt gtcggcgcta ctgccttata tcgaattaaa agaaggcgta
     3061 gacgaagaaa agctctatca agtattaaat cgtaaaatga acaaactttt agaaaagaag
     3121 gaggatcaac gtcctatgct atctgaatta ttaacagaag aaatgatcca attatcggat
     3181 caaccaaaaa actggcaaga agcaatcaca tttgccgcac agccgctatt atcagaaaat
     3241 aaaatagaag aaagttatat caaagctatg atcgagcgcg tggagcagta tggtgcgttt
     3301 atccatattg gcgatcacat cgcattacct catgctcgtc cagaagatgg cgtaaacgaa
     3361 gtagggatgt ctttgctgaa acttcaacaa cctgtggatc tgcttgataa tccagcccat
     3421 cccattagtt tgttcatctg tttagctgct atcgataatg aggcacatct tcgggctttg
     3481 gcaaatttaa caaagctact ttctaataaa caaaatcttc aagatttatt acacgcatca
     3541 acaaaagaag aaatattagc gatcataaga aaaggagacg aatgatatga aaatttttgc
     3601 agtatgtcaa agtggattag gaacaagttt tatggtagag atgaacatca aacaagcact
     3661 tgcagatctt ggagcagatg tggacgagtt tgaagtaagt catacggatg ttggaagtgt
     3721 atctagcgat atggctgatt atttcttcat tgagcattct ttgaaagata cgctaacttc
     3781 tatacctgat gaaaagctcg tacctttgca atcgatcatt gacagcgaag aagtaaaaga
     3841 aaaagtaacg aagatcttgt caaaagaata aaaatgattc aagggaggaa ctatcaatgg
     3901 ataaattgtt gaatttgttt gtgtcaatcg tcagtcagcc ctccatcctc gtttctttga
     3961 ttgcactgct tggtctcgtt cttcagaaga agaaattttc agaagtgatc caaggaacta
     4021 tcaaaacatt tgtcggattc ctagtactga tcggcggtgc tggtttaatc agtaattcac
     4081 taaccccttt tgcttcgatg ttccaaacag ctctgcatac tcaaggagta gttcctagca
     4141 atgaagcagt agtggcgatt gctttgcaac aatacggtac acctactgct ttgatcatgc
     4201 tagtagggat gatcgtgaat attttacttg ctagattcac aagatataaa ttcatttttc
     4261 ttacaggtca agcaatgctt tatgtttctt gtctaacagc agtcatttta gtaagcgcgg
     4321 ggtttactgc aaatttgtgg atgatcttat taggcggggt cttcgaagga acactattaa
     4381 ctattacgcc tgctctttgt caaccgttta tgaagaacat cactggaaac aatcaagttg
     4441 cgttagggca taccggaaat atcggttatg cttctagtgg atggatcgga aagttttttg
     4501 gaaacaaaga ccgttctaca gaggacataa agattcctaa aagtttcagc tttttgcgag
     4561 attctactgt atcgatcatg ctcttgatgt cgatcgtata tgtcgcactt gctttgttag
     4621 ctggtcctgg atttgttgaa agagaactaa gcaatggaga caacgctgta atctttgcac
     4681 tgatccaagc tggaacattc acagctgggt ttgttatcgt ccttcaaggg gttcgaatgg
     4741 tgttggcaga aatcgttcca gcattccaag ggattgctaa aaaactcgta cccgattcta
     4801 aaccagcatt agatgttcct atcgtttttc catatgcacc taatgctgta ttaattggct
     4861 tttttgttag cttctttgta ggaacactat ccatgtttgc tatgataggc ttgcaaacta
     4921 cagttatcat tcctggggta gtaggacatt tcttctgcgg agctgcttct ggtgtcttcg
     4981 gcaactcgac tggcggtaga agaggggcgg tattaggttc tgctttcaat agcttattga
     5041 ttagttggct gccattattt atccttccag tattaggaga tcttaaaatc gcttcttcta
     5101 catttgccga tactgattat ctagtaccag gacttgtact tgggaaacta ggagaatacg
     5161 gtcaaatagc cttgacagtt ggaatcatcg ctttcttttt acttgtcgta ttatctagct
     5221 ttctttttgc cggaaaaaga acaaacattg tttctaaaga acagaattaa gcaaacttca
     5281 aaataaaaat tgcttggaca atatcagaag aagatttaaa gtcttctgat atgtcgcaag
     5341 cagttttttg ttttatgcat ttatttttga atagcttgtt aagacaagtg gctgttttgc
     5401 tagaagagga taacttttac cattcatttt cactgttccg cctgttctat cagaagtaat
     5461 cacgatctta tcttttgttt tttcaagcga aatctttcca tctttgatag gtagcgtaca
     5521 attcagtgtt tcaaatgaat ctagattagg ttgtatcata aattcttcat aacctggctt
     5581 tgttggatac aaaccgataa aatagcgtcc taaaagatag attgggcttg ctccccatgc
     5641 atggcataag ctttttccat aaggatctcc atacatactg tacttttcat ttcctgtttg
     5701 agtagggtcg tattcttccc agaaagttac cgcatcatga gataacatcc ctccccaata
     5761 atcaagaatc gtttggtaga cgatattcgt ctgtccgagt ttgcataaag catcttgttc
     5821 atagaatttg aaatatggag tagtaatctg tgtgatagtg tcattcaata agacattctt
     5881 taagatcgat tgctgttggt ctttgttagc aaaatcgaat aaaacagcga aaatattcgc
     5941 atgacgcgtc acatttcttc tcccagattc atagcaatcg ataaaagcgc caagttcttc
     6001 atcccaaaag tagtcaaata ggttttgttt caatagttga tatttttctt gatagagatg
     6061 agtatgataa ttttcgttct ctccatatgt ttcagctaag atatctccgc aagtgatcat
     6121 ggaatgatag gctttcagta ataagacttg ttctgcagca agcgttcctt ctttatccat
     6181 ttcagaccaa tcaataaata tccaatcttt tgctctccca taaataaacc ctaattcatt
     6241 cgtttgctcc atcaagtatc tcattaatga ttgcatctta gggtagacga tgcgtaaaaa
     6301 ttcatagtct ttcgtcatca tatagtggtt ttcgatacca atcaaccaca agatcgaata
     6361 gtcaacgata gtattcatat gctgcttgat ttcattttgt cctcttaatg ctaatatcgt
     6421 tcgtttattg atttcctcgt caaaaaaaag atattgattg atcatgaatg attgatacgc
     6481 atctccagac caaatccagc gatctcgttt gataccgtcg ataaaaaaca aatcactaca
     6541 taacttgaag gcttcttctg atactgacca gatctgattc agtaaagaag aatcgcttga
     6601 aaaagcagct tgtttcgaaa gagataaaaa ttcatgaaaa acttgaagtt gaaccattcc
     6661 agtagaaacc tctggtataa acaaatagcg aaaagcgcgt ttacgagctt tcatttttct
     6721 tgtcattcct atttgcttat agtagcaata atcgatatcc atagcttcta catcggactc
     6781 tccataacat agagtgatag attcgatatg gtcatccaga gatattagtt ctacacagcc
     6841 attcactgct cgcccaaaat cgaataagat tcctccgcca atctccgttt cagtgactgg
     6901 atggacgcat tctttttgat aatagatttg atttggatct tttgcgatat cgacataaag
     6961 cggactccag ccagctggat attcttcaat aaaattactt gctgtccagc caatatctga
     7021 ttttacttcg tctccctcga taaacatcgc tggtagccct gatgtatgtc ctacaaagat
     7081 acgaattttt gctttaccag ctggacaagt cagccatttt ccacatggat gtttttgtcc
     7141 attaatttct acatacccaa ctccttggat cgtcacttta aacatggaag gttgatcaag
     7201 aaagtaatat ctcttaaact tgacgttccg atggcaatca tccatgtacc agtacgctgg
     7261 ccagtcaaaa cctcgttctt cccgttggaa attttgtagc agtccatggc ggatctcaaa
     7321 gtctccagga taccaaagcc atctatcttt atgtttttca tttgtcattt tacacgacct
     7381 ccatcaaatt tattttgttg tcaattgttc ttttatttct tcatctactc ttatcactgt
     7441 ctttctattc gtcctgtgta ctactagaag gctaataagc gcgctaatca acagcaagat
     7501 actgccattg acgaaaacag caagtgtttt actcccaaga gaagcaaaag agagaaagag
     7561 caaaccatat ggtgcaatga aagtacctaa attagcgcca accaataaga ttgaagtatt
     7621 gaaactctcc gcgtacttgg gcgcttggat acttacttgg ttgaataagt aaggaatgaa
     7681 taacgaaaaa gaaattcctg ttaggatcgc accaaaccca atcacataca ggtttatcac
     7741 agtggcaatc aatacaaagc cgattcccat cagtagtaaa gaaagtgtaa gcatatagcg
     7801 atgtgctttt gtctgaagcc agccaaaaca aactccggca gccatacttg ccaattgcat
     7861 gattgttaag atattcgctg cattagtttc gtttcctaga gaataagtcg tcaggactgt
     7921 ggctaattta atcgatacag cattatatac cacgatgacc agcaagagaa aaataccata
     7981 actaatcatt ttaggattcg ttttgattac ttgtcttgtt ggggctgtgc catgcgattg
     8041 aagttcagtt gtttcaggtg taggaacaaa caaaacaaac aagacaagaa taggtaagct
     8101 gatggcgtag actaaaaagg tatattgcca accgaactgt actagctgtc ctgcaacaaa
     8161 tgtcattaaa gctgcaccaa atccttgaaa tgcactttga aaaccaatca tagaggctcg
     8221 ttcatctccg tgataaaagg cagcaatcat gctgactgcc aaagaattga tcaatccaaa
     8281 gccaatacct agtccagctc ttgaagctaa gacaatactg taactttgtg agaagagggg
     8341 gaggatacca caaatcgatg caatcactaa tccgatgatc acagtttgtt tttgtccgat
     8401 tttcctagca ataaaactac taatcaaaac aaaaatagtg accataaaag agggagcagt
     8461 cgttaataat tcaacagcag ataaagaacg cgtggcataa gtagctgaca tactgggaat
     8521 tgcagacgtg actgcaccag cagaagtcaa aataagagag actgacagca gagaaaattt
     8581 catcatcttt ttattcataa gcatgttcct ttcatttttg taagcgctta tcaaaaatat
     8641 agtatgaagt ggttattctc tcaatagcgg aatcacagga agttcatgtc ccaaccatag
     8701 aaagtcttta tgaaaccaaa aatagctttg catataatag cagtacagcg tgaactaata
     8761 agcgaaagaa accgcttaca aaaaggaggg agtcaattgg aactagctga attagacatt
     8821 ttttttgcgt aacgaagaat caatcgaaac tctgcagcgt gaacaaggaa tgaatatcaa
     8881 tgatttaaac ttagcttacc atcttcagac accgccaaaa atccaccgag gggatctatt
     8941 tcaacaggga aatattcata tcagtaaaca ccgtcgatat gcggaagttc ccgagcatac
     9001 acatgatttt gttgaaatca attatatgta tagcggtaca tgtttacaaa ttattaatgg
     9061 agaaaggata gagatggaag aaagtgacgt ggtgatcatt gataaagaag ctactcaaag
     9121 aatcgaatac acagggcaag aggatatttt aatcaacatc ttattaaaag aagaaacctt
     9181 atctacagag atattgaatc atttggcaag cacaaataat ctactttctt cttttttgat
     9241 aaatgcatca aaagaaaaag gggaacatac acaattcatt cattttcgcg gcggaaaaac
     9301 aagtcgtttg acgcatttga ttgaaaatat gctgttgatt tattttgata catacagtca
     9361 taagatccgt tcgttaaatc tctatacatc actattactg atcgaattga ctcatttatt
     9421 agaacaacaa agcttttcat cagcagatgg atgcgcagaa aatgagatgc ttagagtatt
     9481 agattatatc gataagcatt ttacttcttg tacgttaaaa gaaacagcag ctcattttgg
     9541 ctacaatccg gtctatctta gtaataaatt gaaaatgtat tttgatgctt cctttcaaga
     9601 actggttcta aagaaaaaat tcgactttgc gttggaactg attcaagaaa cgagtttttc
     9661 gttggaagaa atcgctaatc gtatcggata taaacaaacg acttctttat tcaaattatt
     9721 caagaagtat tcaacatgta ctcccaatga atggagaaat aaacattaag aagaaaatct
     9781 ttcactaaag catatgaagg aaaattgttt taggtgcgat tgctgtctta ttatatcgtt
     9841 gcagcgaatt tttttggatt tattttcagt tctaaacttt tggttgtatt atcaggggat
     9901 ataggtgtaa acatttgtca tttatctgtt atgcgtattc aggaattcgt ttccatcaaa
     9961 aattataata acaactatta taattaacat gttgataaaa tgaataaaca agactatact
    10021 tttcatgaaa gtaacaaata gttatgaaaa ggagctgttt attcatgaaa aaaacaagtc
    10081 gtaaagtagt aatcgtcggt acaggttttg ttggaacaag tatcgcttat gcaatgatca
    10141 accaaggaat ctctaatgaa ttagtattga tcgatgtgaa tcaagaaaaa gcagaagggg
    10201 aagcattgga ccttttagat ggtatggctt ggggcgatga aaatgtggct gtatggtcag
    10261 gtggctatga agaatgtaaa gatgctgata tcgttgttat cacagctggt atcaaccaaa
    10321 aacctggtca atctcgttta gatttagtta aaacaaatgc gtcaatcatg cgccaaatcg
    10381 taaaagaaat catgggatca ggattcgacg gtattatcgt tgttgcttca aacccagtag
    10441 atattttgac atatatcgct tggaacgaat ctggtctgcc aacatcacgt gttatcggta
    10501 ctggaacaac attggataca actcgtttcc gtaaagaaat cgcattgaaa ttaaaagttg
    10561 acccacgcag cgtccacggc tatatcttag gtgaacatgg tgattcagaa gttgctgcat
    10621 ggtcacatac aactgtcggt ggtaaaccag tgtttgaaat tgtagaaaaa gaccatcgta
    10681 ttgcccaaga tgaattagat gtgattgctg ataaagtccg taatgctgct tatgagatca
    10741 ttgatcgtaa aaaagcaact tactatggta ttggtatgag tactgcacgt atcgtaaaag
    10801 ctatcttgaa taacgaacaa gctgtattgc ctgtatctgc ttatttaact ggtgaatacg
    10861 acgaaaaaga tatctttaca ggtgtgccat caatcgttga cgaaaatggt gttcgtgaag
    10921 ttgttgaatt atcaatcaac gaagaagaaa aagcaatgtt cagcaaatca acaagtgctt
    10981 taagagaagt attgaacact gtattataag aaacaatgaa tatgaact
//
LOCUS       DACEUZ010000093        10767 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.093, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000093 DACEUZ010000000
VERSION     DACEUZ010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10767
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.093"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            79..1125
                     /locus_tag="IXG44_001976"
     CDS             79..1125
                     /locus_tag="IXG44_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAQ1658687.1"
                     /translation="MTKPNHIESSKKEAPKTEGQTKTFSTPQQIESTESFQSQPILSG
                     NEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIAQAILESASGQSQLAQAPNY
                     NLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYENYEESFEDYAKLLTKGISGN
                     KDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNELIKTYDLTNYDKEVKGPQMS
                     KTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIHAVKAGKVVKAEFHYSWGNY
                     VVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGSTGNSTGPHLHLEICKDSSLS
                     QSMLVDPKTLLFGD"
     gene            1474..2106
                     /locus_tag="IXG44_001977"
     CDS             1474..2106
                     /locus_tag="IXG44_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HAQ1658688.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            2694..4133
                     /locus_tag="IXG44_001978"
     CDS             2694..4133
                     /locus_tag="IXG44_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1658689.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            4428..4646
                     /locus_tag="IXG44_001979"
                     /pseudo
     CDS             4428..4646
                     /locus_tag="IXG44_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174271.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            4895..5110
                     /locus_tag="IXG44_001980"
     CDS             4895..5110
                     /locus_tag="IXG44_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1658690.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(5327..5583)
                     /locus_tag="IXG44_001981"
                     /pseudo
     CDS             complement(5327..5583)
                     /locus_tag="IXG44_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(5770..6681)
                     /locus_tag="IXG44_001982"
     CDS             complement(5770..6681)
                     /locus_tag="IXG44_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ1658691.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(6700..7704)
                     /locus_tag="IXG44_001983"
     CDS             complement(6700..7704)
                     /locus_tag="IXG44_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAQ1658692.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(7701..9824)
                     /locus_tag="IXG44_001984"
     CDS             complement(7701..9824)
                     /locus_tag="IXG44_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ1658693.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(9829..10749)
                     /locus_tag="IXG44_001985"
     CDS             complement(9829..10749)
                     /locus_tag="IXG44_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ1658694.1"
                     /translation="MPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILT
                     FLTGISSRDGEKFPVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTD
                     YDFAHLLFSDGSITQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVIS
                     TFALDFIHSNREIFKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDD
                     LLDERLKNNIGLKFAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLY
                     GRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         3293 a         2032 c         2018 g         3424 t
ORIGIN      
        1 tcaagtgaaa gtagtacgat ttcaagtacc gaatcttcaa caacttcaag tagtacaagt
       61 gaaataactt cttcgaaagt gacaaaacca aaccatatag aatcttcaaa aaaggaagcg
      121 ccaaaaacgg aaggacaaac aaaaactttt tcgacaccac agcaaataga atcaacagaa
      181 tctttccaat cacaaccaat actatctggt aatgaagaaa ttcattttga caaaaatgaa
      241 tctgtagaaa attttattat gaaaattggt gagtctgcga gaaaaattgg tcagaaatat
      301 gatttatatg cttctgttat gattgcacaa gcgatcttag aatctgccag tggtcaaagc
      361 cagttagctc aggcaccgaa ttacaattta tttgggataa aaggaactta taatggaaac
      421 tttgtcatta tggtaacaaa cgaagatttg ggtaatggaa cgctttatac gacccaatca
      481 aaatttagag tgtatgaaaa ctatgaggaa agctttgaag attatgccaa attattaact
      541 aagggaatta gtggaaataa agatttttat gcaggagcat taaaagctaa tagtaaaaca
      601 tatcgagaag caactaaatt ccttacagga cggtatgcaa cagatacaca atactatttg
      661 aagctaaatg aattaattaa aacctacgat ttaaccaact atgataaaga agttaaaggt
      721 ccacaaatga gtaagactgg ataccaagtg cctattcata attattcaat aaccagtttg
      781 tttggaaatc gtggaaatga atttcaccga ggacttgata tggcagctac acagggtgag
      841 ccgattcatg cagttaaagc tggtaaggtt gttaaagcag agtttcacta ctcttgggga
      901 aattacgtgg tgatacaaca cgaagatgga tcaaccgcgt tatatgctca ccagcaacaa
      961 tacctagtaa aaactggaga taatgttttg caaggacaaa taattggcta tgtaggaagt
     1021 actgggaata gtactggccc acaccttcat ttggaaattt gcaaagatag tagtctttct
     1081 cagagcatgt tagttgatcc gaaaacctta ttattcggag actaaaaaag gtaatcaatc
     1141 tgacaaagta acaagaaaat ggttggattc ttagaccttt gatcaggata ttgagactgg
     1201 aattgatatc tcgattacga tgggtttgac aaataaaaca agtccattat cgaatatcga
     1261 gtgtattatt gccgtcttta tgctcatatt ttgagcaggt ttcactagat gaaaacaatt
     1321 tatctatctt gaggatttcg tgtacgtatc gttcagtctt gtattgtaaa ttagtcataa
     1381 aatcagacta agaaaaattg gaaatatttt tttctgattt attaaaagtt aattgaaatt
     1441 atatggtact tctttcttgg gagggtaggt gcagtgaagg aaagatatgg tcaccttact
     1501 gttgttgagg agattactca taatggatat aaaaaatatc gttgcctatg tgactgtggg
     1561 aatgaaacga ttgtatatcg atcaaactta ctatcaggta gaaccaaaag ttgtggatgt
     1621 ggaaattata aaaataggca taaatataaa aatttgacaa ataaaacttt tggtaagtta
     1681 gttgcaatca agccaactaa actgcgaagt gaaggaactg ttgtttggga atgtcaatgt
     1741 ctgtgtggga gaaaaaagtt agtatcggca aagcgacttc taaaaggtga tgtaaaaagc
     1801 tgtggatgtt tgaaatcaaa aaagcatcac attgaaaata aaaaattcgg attacttact
     1861 gcaaagtatc ctttggacaa taactgttca tggaaaacaa aatgggtttg tcattgtgag
     1921 tgtggcagta tgtgtgagat aagttatagt aacttatttt ttggacatac tcaaagttgt
     1981 ggatgtttaa aaaggcaaga aacaaggaca tgtgttgaag ggacagtaat agagtgtcta
     2041 acgtcaaaat taccgaaaaa taatacaagt ggtgttaagg gagtttatcg ttctaaagag
     2101 aaatagattg cgtatataac tttaaaaaaa aagaggtact atttaggatc gtatgattct
     2161 attaagagtg cagctcaagc tagaaaagat gctgaaaata aattgtttca acctttgatt
     2221 gacaaatatc gaggtaatca ttaattattg tggataataa ttaacagtga tatataaaca
     2281 tctataatat attattcata ctattgatat ttagtataat cctatggaat tgaatatcat
     2341 agcaatgtat ttgaaaagca aattggaaaa caaataggtg caacaccata aatatgaaaa
     2401 aattgggaaa aagtagaatg gaaatttgct tacgtctcac gagatagctc acactattaa
     2461 tctgaagaac aatgaagtcg acttttagaa attgctagaa gaaagtaaag cgaattatca
     2521 ctagttacta tagagtaagt tcaaataaag catctccaac ttacatggtt acatgaggat
     2581 atcaaatcga aattactcaa gcttcctatg gctagagtcg cgagaagttg ttgggcagta
     2641 gacataggtt atattgttct tagctacacg atcattagaa aggaaatcct attatgtata
     2701 gttatcaaga agatgcaaaa gtccaaaaaa atcgctggtg gattttgatt gctatatcta
     2761 tgtttacctt catgtccaca ttggatggca gtattgtcaa tattgcacta cctaccatct
     2821 cagatgatat gggcatacca atgaatcaag cggaatgggt agtgtccgtt tatctgatga
     2881 ttgtctgttc ttgtttgttg ctgtttggta aaattggcga tagccgtggg aaaatcaaag
     2941 tatttcggat tggtacgctg atctttgtct tgggatcgct gttgtgtggg ttcaatcatt
     3001 cgctaggtat tttattgttc gcacgggtgg tacaagccat tggtgctagt atgactatgg
     3061 ctacaaatgc cggaatcatc acggaagtct ttcccataca tgaacgtggt cgggcattgg
     3121 gagcaatagg tgcgtttgtt tcattaggct cgattgctgg accaggtctt ggcggcttga
     3181 ttttgtccca attttcttgg gcgtacattt tttggatcaa tgtaccggtg ggaatcgtaa
     3241 cgatgttgat tgctgaaaaa ttcttaccaa aagatatcac catgagcggc catacaatcg
     3301 atggaggggg ctttgctgcc attgcggcta cgattatcag cttttttgga gcaatctttt
     3361 tggggcaaga aagtggcttt ttccatccag tcccccttgt actgttcgta atttcagtga
     3421 ttgcctttgt cagcttttta aaagttgaaa agaaagcagc ctctccattg atttctttcg
     3481 gtatttttaa gaacaagatt tttaccatga gcttgattac agccttgttg attttttctt
     3541 ccaacttctt tgtcaatgta gtgattcctt tttatctgca aaatgctcgc gggttgagtc
     3601 ctagctatgc tgggctgttg atgatggtct ttcctttgat gatggtcgtc ggggcgcctt
     3661 taagcggcta cctgaccgac cgggttggcc cggagatttt ggtattagta ggcttgggct
     3721 tattgtcggt aacccaggtc atgtatatgt ttttgtcagt ggactctcct ttgtggctat
     3781 acattgtcgc tactgccatc atgggtgccg gcaatgcctt gttccagtcc cccaataata
     3841 cgatggttat gagctcggtc actatcgaaa acttgggact ggcaggctcg atgaattcct
     3901 ttgcccgaaa tttggggatg gtcagcggga tttcgctttc tacgacggtc ttatatcaag
     3961 cgatgtctgc taagatgggc cagcaagtaa ctaactatat cgcaaatcgg ccggatgtct
     4021 ttttatatgg aatgaagatt acctttttag gcagctttgt tttgtgtttt gttgcctttt
     4081 tcctgactat ctggcgtatc aaaaaacaag gaggtattca gaaaaatgtc tgaaaaaacc
     4141 actgttgaag aatttttgga ggcctgcgta tgggtgagtg ggatttccat ggatgatgga
     4201 agatgaagtg aacttctaaa agccgaaaaa ctcaagtatt atcttcaaaa tactagtgct
     4261 caatgagatg cttgatttga tcacaagaag actgcgaaag ccgctggcaa ccgcaagcta
     4321 ggtatcccac cgatggacta cgttttagag gctactggtt atgaatacgg agccaacgct
     4381 ctcatctgca taagaataca tcacccagat tatgtaaaga ttttcgatac ttctgtcaaa
     4441 aaatcattaa gatagataaa tgatttccgt ctagtcaaat tggttcagaa tgtagacaca
     4501 cagatggtaa gaagtcactc acaattcgag aatggacttg tcctatttgc cgtatacatc
     4561 ataatcgaaa cgtcaacgat agtatcaata ttttgaccaa gggctatgaa tacaagcatt
     4621 gccttagaaa acatcaagaa ccttaggaac aacggggata gcttggttaa taagagatac
     4681 cgctgtttag gcttgcaagt atactctttt atcaagaatt ccgtggcttt agtcatgaga
     4741 ggcttaatac tatgaaaatt ttctttagca tttttgattt aggttccttt tctaattata
     4801 ataagatgac cactacagta acctactgaa atatataaac agccactgta gtaccatttt
     4861 caaagtagtc tatgagagga gaatcttcat tcttatgaac gctggaattg cggatatgaa
     4921 tttaataaaa aaaaccctta atgattttac ttccaattca atatcaaaag gtactggtat
     4981 caatttaagc acaataaaaa agctgaaaag tggtgaaaga tcagtggaaa agctgaatct
     5041 tttagatgct ataaaaataa cggaatttgc tatgaaaaat ggaaaagctg agattgaaat
     5101 atggagataa ttatcgtcta gtgaattact tgccacttag ctgaatttaa cgatccttgg
     5161 tgcttgaagg gggagattct tgggaataga gcatacttgc aagcctatgt ctttactaac
     5221 aacgatatct cttattaacc aagctatccc cgtagttcta aggcttttac ttttatctaa
     5281 gcaataatcg tattcaaagg aaatttttga cccttagaaa gtctcatttt atcagacgtg
     5341 agcttaggca ttgctgaatt ttgttaattc ggttttaggt tgtgctttga gcataatatg
     5401 aaagtattct ttatcacgat ttcattcatc taaggtaatg tgatgtgatt ctgaaattct
     5461 ttcaaaggca gtttttgcat aagctgacat tacatcatca atgacatgtt tacaatgttt
     5521 cactactaaa ataagggggt aaccaagaag aaaaactgaa tgattatttg tatctaattc
     5581 catggtatat caaccatttc ctatatagac tgattatgac atggattaaa ataaaaaggc
     5641 actatgtctt aattgtcggt tttcgagtta ccgaatggca ttcatcaccc acctatagag
     5701 gatgggcgac ttctgcctga aataagttac actacttata acttctaata aaattagatt
     5761 gagtcattat tatttaacat caacgatttt ccagtttcca tttttttgta atactaattt
     5821 gtactgaaag tagctagtgg tttttgatac attatcaagg tattttatct ttgtactcac
     5881 tagaaaattt tttccgtttt gtagataaac tggattctct aactccgaaa actttaagtc
     5941 aatattgatt ggtcgcatcg caccattttc aacataatac tccaattctt tttcagaagc
     6001 tgatggataa agtttaaaaa atgtttctaa aaaatcggtg atttcttccg ttgttttggc
     6061 atcaatttct gaatcgtttt ctacttgttt tgcttcatat tttgccttgt ctggctttcc
     6121 aatcattgtt ggcaaactag ttactaaatg attcctgttt tcatcttcaa agattgtcac
     6181 tcgatatgca gaagaaattg attgcgtctt ttttccctct gtaatatgtt gctccactaa
     6241 aaatgaaacg acaaattctt ccgagtgttt ttcaacatct agaattttta cagattgaac
     6301 ttcggaactc gtgggaatat ctgctcgaac catatcttgg attaaagcga gaccgttttc
     6361 tgtaaggtac tgttcgagat tactcattct cttttcaatt gcttctttgt tatttttcca
     6421 tgaaaaatat tctttcgcaa aatcctctgt aaaactttca acgccagaag tattcactac
     6481 cttctctttt ataaccactt tttcatgagt tgtatgttga tcaatggcgg tgaaattttt
     6541 ataaacgcca aaagcaagac tacaagacaa taaaagccaa agggcaagca ccatttttcg
     6601 atgcttaccc acggatacaa ctcgttcttt tttctttttt gggctctttt ccttttgatt
     6661 ccgttcaatt tttatcttca ttctttttcc tcttttctct tatttgtatc ggccagctcc
     6721 gataagatgc tgccgccaat agttttccgt tagattcgtc caccccactg gatctcctgc
     6781 atgaaacatg cgattctccc ctgcgtaaat gcccacatgg gtcacataag taccggcatc
     6841 ataggttgaa tgaaagaaaa ctaaatcacc tggtttggca tcttttagat caatatgagt
     6901 catggcatca tattgatcct gagccacccg aggaagtttc aggtcaactt tggcataaca
     6961 ccattgggtc aagccactac aatcaaaccc cgtttctgga cttgagccac caaacacata
     7021 ggccgttcct tcatacttaa atgcctcatc aaaaatttct tggactattt tgtcatcaaa
     7081 ctgagttgta gttaggtatt gcttcactaa taaacagtaa aacatattgc cataattata
     7141 ccgccaccca ccattttcct taatcgcgat tgggttgggg taatcgactt tttgaccacc
     7201 tgatttttcc tttgaaaaag attcagctaa agcaaaacta tgcttcttcc cgtttttccc
     7261 gacatgatct agatacccgc caccatagtt ataggcctga ataatactat cttgatcaca
     7321 ccctagttct ttggaacgct taaccaaaga cgcaaaatag agacagcctt gtttgatgga
     7381 tgcctcggta cttaatgaat taggtggtaa tccggctgac tcagaacttt gcataacgtc
     7441 ttccattgtg ccaccagatt ccacttgaat aatcgccaaa agaattggaa tatactctgt
     7501 tatatccgat tctttggcat acttttccac cattgcacgg tgtttcaata cttcttctga
     7561 gacactagtt ccgccaggag aagtgccaga actaccattt ccctcactgt cttcaccaaa
     7621 caacaaaccc acacacatca aaatacccat aaaagagatg gtcagaaaag ttaatgaaat
     7681 aacacccact ttcttcaagt tcattttttc tcacctgacc ttgattttgg acgatggttc
     7741 attctttttt gtactttttg attggtctgc tttggcagag tggacctctg tttcacttgt
     7801 tggaaagaat ttttcttact tgctgattgg cttttccctt ttgaaatgat ttcttgacta
     7861 gttagttttc gtttgatttc gggattcaga tttgctctca caatccgctt tggtgcttct
     7921 tttttcagca tcatatcttt ctgaggttga gctgctttat ctattggaat attcgccctt
     7981 ctttttccct gacgatttgt tgcttcactc agtatcttgc gtttttcgtc cattttcgca
     8041 tttcttcgtt gtcgttgttt ttctcgcaaa tccactcgat ccgctttttc ttggaacagt
     8101 ccgcgcttga attcttctgg tgcttttttc gacttttcga ttcctttatg gagattgtat
     8161 ttcaaattcg ttggcgtatt ccttacctga tcctttactt gtttagtctt atctttcacc
     8221 aaagcttggg tatccaaaac tttgccgatt tttctcccag ccaaattcat tcgggattgt
     8281 ttcttattat ttgaaactgg attctcctta gttaagtcag atagtatttc gtgatcgttc
     8341 ggtaatcgtg aattctttcg tagagaagaa ccggatggct taaatttgga ttttgattgt
     8401 tcctttgcca acttatttcc gactgctgca ccaccaccaa aagccaaagc tcgtcccaca
     8461 tttcgattga gttgccgcaa ttttcgattc attaacattt gcggttttcg catgacgcgt
     8521 cttcccattg actggctatc attactctgg aggttgaaca tactcataat gtcgcccaat
     8581 ttgaaataga taccggcaaa ggtcacaatt tgcagaaaag caaccatgaa aaatggatag
     8641 gttgccgaaa tattgaaaaa catcgtggag atgctaaatg cagtcgtaat caccaaagta
     8701 acacccgctc gcatcataat cgtattgaat aaacgaataa tcgccttctt tcctaaactt
     8761 tcataagtcg gcaacatgga caataagaaa ctaattggca gaaacatcgc aaagataatg
     8821 aagagaattt gcgagaacaa cataatccct gttagaagaa aaacaaagaa ggaaatgcca
     8881 atattgaaca ataccaaaaa gaccacggtc cctaaacggt tcatggtctt ggtaatactg
     8941 agattcgcat tgtcattgtc ttcaatctct gccttgaccg cttcttcccg atctttgcct
     9001 ttgttttcat ctggactcac agatagaatc ttgttgaccc ggtcttcacc aatttcttct
     9061 atgttggaat catcaaattg cagtagtaac caaggttgtt ggacttgaat cgaaaacaaa
     9121 ctatcccgaa tcaaatccac actatctttt ccttggcttt ctgaattggg tacaacgatc
     9181 tcggttccaa gggtaagggc cgcttcacta acatctgagc taaagtcatt gatttttgtg
     9241 atataggtag gcgcataagc aatgaacgaa cccgatagta gaaaaatcat cacaaaatta
     9301 atcacggctc gaactgcttt cgtggtttct cgttttaata atccggtata cgccacatac
     9361 acccccagag ctaaaatcat caataataga aacccaaaat aaaatccgga ggtctggagt
     9421 ccattttcag taataccagc caatgtttgg atattctttc caatactttc agccgtatcc
     9481 gaaatgaaat ctaatttgta agcctcttga accacataac ccgtggcgtt cgacaaatac
     9541 aaactcaccg tccaaatgaa attagtaatg gcataaagac catacatcac gcttttccct
     9601 aagccatcac cccaattcca aggcaaccaa tcccaagagc tatccacaaa gaaatctagt
     9661 tgatagttgt tcagtgaata tttggaatat agatttcctg cttcaactgt atcatcaact
     9721 agccctgtcg cctcggctac cgtaccaact aaactcaata gaactatcag agtgagtatg
     9781 ctaataaaaa cgattcccac aatccgaaaa agtttctttt tcatcccctc actccattct
     9841 ctcttgaaca ggtggacggg tatcaaaggc atggaagagt tcttcaaaga ctggatgtac
     9901 ctgaaccacg cctacccgac catacaaatc ctgcatcaag cattggccat tttccaaatc
     9961 tcgtaggcgt ttttgattgc tttcatcttc tcgatccaag ccaaagaatt ccaaggtttt
    10021 cttgatttca tgaatatcgg tactgcgaaa cgcaaacttc aacccgatat tattcttcaa
    10081 gcgttcatcc agtaaatcat cactattttg cgtgacaaag taaaccccgg cattcatagc
    10141 ccgcccagca cgaacgagct tattcgataa tgttttccct tgcgctactt gaagaaaact
    10201 ccacgcctca tctaagtcta caattttgaa gatttctcga ttactatgga taaagtccaa
    10261 ggcaaaagtg gaaatgacga ttagcatgga aacactaagc aattccatgg tggtatattc
    10321 ttcaaaagtg gtttctgcat ctggtaaaac caaatccgct acttgaatga tatttaactg
    10381 cctatctaaa ctaatcgact gggtaatcga accatctgag aaaagcaaat gggcaaaatc
    10441 ataatcggta aaggactcga tgtgatccgc gatattttcc gcaactggtg agccttcttt
    10501 tcgaagctca ttaattactt gtaataatcc ccgttgattg ctttgggtaa ccgtccgaat
    10561 ggccttacgc aacactggaa acttctcccc atctcgacta gaaattcctg ttaaaaaggt
    10621 cagaatatca attgctagac tttcagaatc cttctttttc tccataatca cataaggatc
    10681 aagtaatcct tgattttttg gcttactagt taagttgaga atattgattt catccgcaat
    10741 ttctggcaag gtttctttcc agccacc
//
LOCUS       DACEUZ010000094        10597 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.094, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000094 DACEUZ010000000
VERSION     DACEUZ010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10597
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.094"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            167..973
                     /locus_tag="IXG44_001986"
     CDS             167..973
                     /locus_tag="IXG44_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1658695.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            985..2205
                     /locus_tag="IXG44_001987"
     CDS             985..2205
                     /locus_tag="IXG44_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1658696.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     NKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            2195..3781
                     /locus_tag="IXG44_001988"
     CDS             2195..3781
                     /locus_tag="IXG44_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAQ1658697.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVVQTSIAQPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSEAT
                     ERMVFRFAQQPSFIEFSAEAAGEKLIFIDSNAVVKTVNDCYLFDGKEIPEFALTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENQHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKKTSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSRDEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            3820..5958
                     /locus_tag="IXG44_001989"
     CDS             3820..5958
                     /locus_tag="IXG44_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ1658698.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKIRLSGKMRGKAILDFQTAKHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKTIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRQENGHISLATNSALEMAEGEFIALLDNDDELPPF
                     ALYEVAKVLNIHPELDLIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKKLGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYPNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTAMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPSYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            6246..7637
                     /locus_tag="IXG44_001990"
     CDS             6246..7637
                     /locus_tag="IXG44_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAQ1658699.1"
                     /translation="MNKNGEWNAARRIFIIIMDVLVLNVSIYLSFLLKFQGEVPARNL
                     ETFQHSAVFISIIFIALNILLGAYVFYSRVISDIVFITVIGQVLIALGIMVVTFAGRW
                     FAFPRGVIVLSFILGTILLSVYRIVVYKLYLRVSRDKKVLIVGLEKDVAPAIQNFQSK
                     KNNKHKVEAVVIDKYYENTKKLIDDIDIVYLASKIPEDEKINIYQLVTKKEKKLFLNT
                     TFENLTMINPNIMNFEDESIIEASGFKIQPEYELFKRLFDIIVSLILLILASPFMLIT
                     AILVKTTSPGPIIYKQVRITKNQKEFSIYKFRSMSVTAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNEENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLLLDLKILLQTIKILFDKMSSQGLDEDENIRS
                     LAEIDFSQEKIYR"
     gene            7696..8505
                     /locus_tag="IXG44_001991"
     CDS             7696..8505
                     /locus_tag="IXG44_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ1658700.1"
                     /translation="MLLSLYDKEKPEYLKEALTSIFEQTVMPKEIVLVYDGPINEKLQ
                     VVVTYFQQKDPHLFTVVKLEKNQGLGIALAIGLEHTKNRLVARMDTDDIMEPQRLEKQ
                     LEIFQKDPEIAIVGSNIEEFTGTLSNVIGRRIVPEYNDEIRVFSKRRNPFNHMTVMFD
                     KNAILEVGNYRPLIGFEDYYLWVRLLKAGYKGYNIQEILVHARAGADMYARRGGSKYL
                     LPGIKGRYRVWKDGLGSLKDFLIVICGHLVISLLPNKIRGKVYESKLRKTN"
     gene            8477..9466
                     /locus_tag="IXG44_001992"
     CDS             8477..9466
                     /locus_tag="IXG44_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ1658701.1"
                     /translation="MNQNLEKQTKLSIIIPFYNAKNTLKRAVDSVLTQGYPNIEILLI
                     DDGSTDGSSDIGDEIANLESKVACYHLENAGPSVARNCGIKEATGNLVGFLDSDDYFL
                     PDCFNNVLKDYHRNYPDIIAFGLSKGKNIENANLFVPEKKIGCSSEEAIEQMFRSKAV
                     DFYVWNKFFKRELFNHIVFPENKLYEDMVPMYEAFRAASKIDILPFSGIFYYQNNDSI
                     VYQSFNSKQYDNILQRKILVDEVKKDYPRLINLANARLVDGYLSTGFKITGKNQNQKE
                     KQKYYNQSKREIKSIYSKIRSNNEISIAKKLALFLYLINPKTYFILYKKVLKK"
     gene            9580..10575
                     /locus_tag="IXG44_001993"
     CDS             9580..10575
                     /locus_tag="IXG44_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ1658702.1"
                     /translation="MHKISVIVPAYNVEDYLENCLESISNQTHSNIEIIVVNDGSPDR
                     SGEIAEEYKKKDMRLKVIHQVNGGLSAARNSGLDSATGDFVCFIDSDDWIEPDYLELL
                     YFGTQTYGADISVLKIKKISSLEQLKEHTIAERKWEIFDREQAMKNLFINNKIGYSAN
                     NKLFNRNLFDNIRFPLGKLMEDKATTYLLIDHSEKVAVNSSAKYHYYQSPKSILRGSF
                     NPRIFDSFEVHEDILIFIDKFYPELSINVRTRYVYEAIRMMMTMIKNNYTNKEDYLRS
                     INILQRFEKEVKIDPDFSPKLKKLVKFLLINKNLPYYMSSSKIASKFLIKIESVR"
BASE COUNT         3723 a         1573 c         2092 g         3209 t
ORIGIN      
        1 ttttttggtt tattgcaatt gtctagcatc ttctgtaaaa tagaaacgtt agagaaaatc
       61 ttgtatcata gaaaattaag aaagaaataa gaacgcagct cggtaccaaa aatatggtaa
      121 aatggcactt gactgtaatt cactgaaatc gcttggagga acacaaatgt ttaaagattt
      181 gtatttattt atgaaagaca tttatcagaa tagaaaatta ctgctacagt tttcgatcaa
      241 tgacttcaaa tcaagatatg caggatcgtt tttaggaatc ttatgggcgt ttatcaatcc
      301 gctatttact gtattgattt attggctggt atttggtgtt ggactgaaag cacaaatgat
      361 cgatgcaaaa tacccattta tcgtatattt agttaccggg atgattccct ggttcttctt
      421 ctcagatgca tttgcatcaa ctacggttgt ttttcgggaa tatacttatt tagtaaagaa
      481 agtcgtattc aatattcgta tcttgcccac gacgaaaatc ctatccaatc tgtacaccca
      541 tttattcttc attctgattg gcatagctgt cgcgcttata aatggtatat atccttcagc
      601 aatgtcttta cagctgattt attatttatt ttgcatgtgt gctttcttga caggattgac
      661 ttggctgacg gcatcgatcc aaccattttt acctgatatc atgcaattaa ttacgatcgt
      721 gctgcagttg atcatgtgga cattaccgat attatggagc cctaatcagt ttgattcagg
      781 tcttgtaagc atattgaaat taaatccgct gtactatgtt gttcaaggtt atcgtgagtc
      841 atttttaagt gaagcttggt tctgggagca tggaacatat actttgtatt tctgggtttt
      901 cacgttagta atgttcgtaa tcggctctgc agtgttccgt agattgaaac cacatttttc
      961 agatgtgctg taaggagaat agctatgaca gaatatgcaa ttagactaaa agatatcaca
     1021 aaatcttata atatgtacgc aaaaccttcg gatcgatttc gtgaagccct caatcctttt
     1081 aagaaatcct atcatgatgt attttatgcg ttgaaaaacg tgaatgtaga aataaaaaaa
     1141 ggtgagatga tcggtttcgt gggagaaaac ggatcgggaa aatctacaat gttgaaaatc
     1201 attacgggag tactgacacc aacatccggc acgatggaaa tcgaaggaaa aatctcagct
     1261 ttgctagaac taggatcagg attcaatcct gagtattctg gctatgaaaa tatctattta
     1321 aatggaatgg ttctaggatt ttcaagagaa gaagtcgatg aaatggtcga tgatatcatt
     1381 gaatttgctg atattggcga ccatatttat caacctgtaa aaacctattc aagtgggatg
     1441 ttcgttcgtc tagcttttgc tgtagcgatc aatgtgaatc ctgatatttt gattgtggat
     1501 gaagcattgg cagtaggaga tcttgaattc caactgaaat gtatggaaaa atttacagaa
     1561 attaagaact cgggaaaaac gattttattc gtttctcatg atattagttc gattcgccgt
     1621 ttttgtgatc gttcctattg gttgaaaaat ggagaagttg tcgaatatgg tgatacgatg
     1681 gaagtaacga cgaattacga aaactttttg aagaaaaaat cagttaaaac agtagatcga
     1741 aataaaaacc aagtagaacg cttcgcagct ccagatatcg tagacgtgac gaaagccgaa
     1801 ttgttggata aaaataaaaa tccagtggat atgctggaac aaggagaaaa actatacgtt
     1861 aaagtaacct atgaagtaaa agacgataca atcaaaaatc cagttttagg aattgctctg
     1921 cgtacagtcg acaattttta tgtgtgcgga ctcaatactc tactagatgg tatcaagatc
     1981 ccttggaaaa aaggtgagaa tatcttttat ctggagtatg aaaagttagg tttgttagct
     2041 ggtgaatatt attttgacgt tgcggtcttt gaagaaaatg caacggttcc tttggtatac
     2101 aagacaaaat acatgaactt gtttgtcagc ggttcttata tcggagaagg catcgtggtc
     2161 ttagaccata aatgggaaga agggactcat agcaatgaaa tatgattttg aaatggacct
     2221 agatgaacaa agcagtgtgg gaaaaattgc cgcacagatc aaaccaggaa gtaaagtttt
     2281 agagtttggt cccggaaacg gtcgattaac gaaacatttg atcggagcaa aacaatgtga
     2341 agtgagcatt gttgagctag ataaggaatt attcgatttt gtcagcgaat ttgcccaaga
     2401 tggattttat ggagatatcg aaagttttga atgggcaaac tattatgctg gacagacatt
     2461 tgattacgtc ttgtttgcgg atgttttaga acatttagta gatcctggaa aaacattgaa
     2521 gaaagtcaga gaatttttga atgaagaagg agagatcctg attacattcc ctaacttagc
     2581 acataattct gtcatgatcg atttgttcaa taatcaattg ccttgggctt cttatggtct
     2641 cctagatgaa acccataatt ctttttatac tcatgatggt ttccaaaaag tttttgaaaa
     2701 agccggatta ttcattaata ttgaagatta cttgtacttg gctgtaggag atacagaatt
     2761 aaaatcaact tatgaagagt tgccggaagc tgtacgctat gatttcaaaa tgcgaccatt
     2821 tggtgaagtc taccaatact ttttctcatt gatgaaacat ccagtagtac aaacttccat
     2881 tgcacagcca caaaactcta attatgtaaa agtactagaa gtaacccagc aaacgaaaca
     2941 agatgaaaca atccaacaaa taccattcaa taatttcaca ggggaaaatg agacactaag
     3001 tttccccgtg tcagaagcaa cagaaagaat ggtgtttcgc ttcgcacagc aacctagctt
     3061 tatcgaattc agtgcagaag ctgctggaga aaaactcata tttatcgatt caaatgctgt
     3121 agtcaaaaca gtaaatgatt gttatttgtt tgatgggaaa gaaataccag agttcgctct
     3181 aacggatatt tctgggaaag aagtgactat ccattgtcac tatcgcttta tcggggaatt
     3241 gacaccaaca atgaaagaat tgctggaaac aattcgtcct atggctgaag tcacaaagca
     3301 gctttctgag aaaaatgatg aattagaaag agaaaatcag catcttcttg aggaaaatac
     3361 aagactagac catacgttaa aaacaaccac aaatcgctat tgcacattgt tggaaagtga
     3421 tgaatggacg atcaaacata gactaggacg acgtaagaaa aaaacatcga aaaaaataca
     3481 agaaaaagag ctttctatat gtattgatga gaaaatatgg gatgcagaaa caaaaatatt
     3541 aaaaatcatc ggatggggaa tcgctaattc ggatcgccag ccattgtcct ataagttatc
     3601 agctgaccaa tcgccatttt tcgaagcaat tcctgtttca cgagatgaag tcaatcaagc
     3661 agaacagctc gcaaaaggaa cagaagcggg atttgaatta cgaatccttt gcgagcaaga
     3721 acgttcattc ttagtggaag cagtagctca aaatggtcaa tcatggatca ttgaaatgta
     3781 aataatgatt aacttataaa gataaggagt aaacgaatcg tgcaaaaaaa agaaattgcc
     3841 gtatttatcg atcaaatcac tcgtgaacgg gccactggcg atttgcttat tgtgggctgg
     3901 gcaattgatg aagtgaccaa agaaatccct acgataaagg tagaaaaaga aaatgtgatt
     3961 gctgaagcaa cacatgtcgt aagattggat atcaatcatt tatataatct tgatgttaag
     4021 acccaaagcg gttttaagat acgtttatcc gggaaaatga gaggaaaagc gatcttagat
     4081 tttcaaacgg caaaacatca aaatggcata gctgtgaaac taaatggtaa atacccttat
     4141 gatgacggca ttgaatcttc atgggaaaga aaaaaaagat tactgaaaaa agggataaat
     4201 tatgcaagga ctcatggcgt aaaaaaaaca atccgccggg tcaaactgga attgaaacca
     4261 ggatcgatcg actatgcaga gtggatcagt cgtcacgaga aacctgatct gaaagagcaa
     4321 aggaaagaag cacaagggtt tgcataccgt ccattgattt ccatacttat gcctgtctat
     4381 aacgttgaaa tcaaatggct cgaaaaatgc attgattcgg tattggatca aacgtatgat
     4441 cactgggaac tatgtatcag tgatgatgct tctactgatc cagctatccg aaagtgtctg
     4501 gaatcctatc aagcaaagga tgatagaatc aaagttgttt tccgacaaga aaatggacat
     4561 atcagtctgg caactaattc tgcgttggaa atggcagaag gagaatttat agcgctctta
     4621 gataatgatg atgaattacc gccatttgca ttatatgaag tggcaaaagt gctaaacata
     4681 catccagaac tggatttgat ctacagtgac gaagataaaa tcgatgcaga tggcaatcgc
     4741 tttgatccgc acttcaaagc agactggtct cccgatacat tgatgggaaa taattatatc
     4801 tctcacttag gcgtatatcg aacaagtatt gtgaaaaagc ttggcggttt ccgtaaaggc
     4861 tacgaaggct ctcaagatta tgatttggtc cttcgtgtga cagaacaaat ccctgcagat
     4921 catatttacc atattgaccg agtcttgtat cattggcgga cgatcccagg atcaaccgca
     4981 agtaatggtg aagccaaaag ttatatctat gattctggag tgaaagcatt gacagatgcc
     5041 ctgtctagaa ggaatatcaa aggaagcgta cgtcctggca gaatttctgg attttatgag
     5101 attgcttatg acgtcttgca agaggatttg gtcagcgtga tcattccaac gaagaatggc
     5161 tatgaagatc taaaaacttg tgtggattcg attatcgaaa aaacgtctta tccaaattat
     5221 gaaatcatta ttgcagataa tggaagtaca gatcctaaaa tgcaggaatt atttgcagaa
     5281 tacaagcatc agctgaaaga ccgatttatc gttgaattaa ttgacattcc attcaattat
     5341 tcacgtatca acaatctggc agcagaaaaa gcaaacggca aatacttttt attcttgaat
     5401 aacgatacag aagtgattga acctgactgg atgacagcga tggtttctta tgcacagttt
     5461 gatcgaatcg gctgtgtagg agcaaaactg ttttatccag atgatacaac gcagcatgca
     5521 ggagtattat taggaatcgg tggtgttgca ggacatgcat tgaataacta tgatcgaaca
     5581 cattgtggtt atttcggccg attggtcatt gacgtcaatt acttagcagt cactgcagct
     5641 tgtatgatgg tcaaggcagc tgacttcaat gctgtcaatg gatttgatga aacattagaa
     5701 gtagcattca atgatgttga cttgtgtttg aaagtttatg aactgggtcg ttacaatgtc
     5761 tatgcacatc aagcagaatt gtatcatttc gaatctaaat caagaggtta tgaagataca
     5821 ccagaaaaac aaagacgttt tgccggggaa ataaaaaaaa tgcaagataa gtggccttct
     5881 tatattgctc atgacccatt ttataacgat aatctaacta aagaaggaat cggcgatttt
     5941 tcattgagac ctgattgatt aaaagaactg tattttcatt tttattgatg aaaatacagt
     6001 ttttttagta tatgaaaatt gtaacttgtg accgaagaga tgataggaag aacacccatt
     6061 cagtaggaag aatgtgatac gatttttgtc caactctctg ttaggcagaa atatacatat
     6121 aaacatacaa tagtcacctt ttctagtttc ttttaattta ttttacaatt tggttattca
     6181 ctaggttttt aaactttgct atggtaagat gatagaggca ttattttatg gttaggagtg
     6241 tatatatgaa taaaaatggg gagtggaatg cggcacgtag gatattcatt atcatcatgg
     6301 atgtacttgt attgaatgtg tcgatctatc ttagcttttt gctgaagttc caaggagaag
     6361 tcccagcaag aaacttagag acctttcagc attcagcagt ttttatttcc attatcttta
     6421 ttgcgttgaa tattttgtta ggagcttatg tattctatag ccgagtgatc agtgatatcg
     6481 tcttcattac tgttattgga caggtgctga tagcattggg gatcatggta gttacttttg
     6541 ctggacgttg gtttgcattt ccgcgtggcg tcatcgtgct ctcgtttata cttggcacga
     6601 tattacttag cgtttatcgg atagttgttt ataaactata cttaagagtc agtcgtgata
     6661 agaaagtcct tatcgtaggt ttggagaaag atgtcgcacc agctatccaa aactttcaat
     6721 ctaagaaaaa caacaaacat aaagttgaag cagtagtgat cgacaagtat tacgaaaaca
     6781 caaaaaaatt gattgatgat atcgatattg tctacttggc ttctaaaatc ccagaagacg
     6841 aaaaaatcaa tatttatcaa ttagtaacta aaaaagagaa gaagctattt ttaaatacaa
     6901 cttttgaaaa tttaacgatg atcaatccga acatcatgaa tttcgaagat gaaagtatca
     6961 ttgaagcttc cggtttcaaa attcagccgg aatatgaatt attcaaacgt ctttttgata
     7021 tcatcgtttc gcttatttta ctgattcttg cttcaccttt tatgctaatc acagcgattt
     7081 tggtaaaaac aacttcacct ggaccaatta tttataaaca agtacgtatt acgaaaaatc
     7141 aaaaggaatt ttcaatctat aaatttcgtt ccatgagtgt aacagctgaa gcaaaatcag
     7201 gaccggtctt ggcaaagagc aatgatgcac gtgtgacacc agtaggcaaa tttatccgtg
     7261 ccgtacgttt tgacgaatta cctcaaatct ttaatgtatt gaaaggcgat atgtcgatcg
     7321 tcggaccacg tccagaaaga ccatttttcg ttgatcagtt caatgaagaa aatccttatt
     7381 attatttaag acacaatgta cgtgcaggga ttaccggata tgcacaagta tacggaaaat
     7441 atgtatcaga ttataatagt aaactacgtt ttgatttgtt gtatatcaaa aagtattcct
     7501 tactattaga tttgaagatt ttacttcaaa caatcaaaat cttatttgat aagatgtcct
     7561 cacaaggact agatgaagat gaaaatatcc gttcgttagc agaaatcgac ttttcacaag
     7621 agaaaattta tcgctagatg ggaaaaattt tgagcaaaga agtaggagag aacattggaa
     7681 aataaaaata taagtatgct gctttcttta tatgataaag aaaaaccaga gtatttaaag
     7741 gaagcattaa ctagtatttt tgagcaaaca gtaatgccaa aagaaattgt attagtttat
     7801 gacggcccta taaatgaaaa acttcaagta gttgtcactt atttccaaca aaaagatcct
     7861 cacttgttta cagttgtaaa attagaaaaa aatcaaggat tggggattgc tttagcaatt
     7921 ggattagaac atacgaaaaa tcgcttagta gccagaatgg atacagatga tatcatggaa
     7981 ccccaacgat tagaaaaaca attagagatc ttccaaaaag atccagaaat agcgattgtt
     8041 gggagtaata ttgaggaatt tactggcacg cttagcaatg ttatcggaag aaggattgta
     8101 cctgaatata atgatgaaat ccgtgtgttt tcgaaaagaa gaaatccctt taatcatatg
     8161 acagtcatgt ttgataaaaa tgctatttta gaagtgggaa actatcgccc attaattgga
     8221 tttgaagatt attatttgtg ggtaagactg ttgaaggcgg gatataaagg gtataatatc
     8281 caagagattc tagtccatgc tagagcaggt gctgacatgt atgctagacg tggaggaagt
     8341 aaatatcttt taccaggaat taaaggccga tatcgcgtat ggaaagatgg tctaggatca
     8401 ctaaaagatt ttctgatagt tatttgcgga catcttgtaa ttagtttatt accgaataaa
     8461 attaggggaa aagtatatga atcaaaactt agaaaaacaa actaaacttt ctataatcat
     8521 acctttttac aatgcaaaaa atactttaaa aagagctgta gacagcgtct taacgcaagg
     8581 ttatcccaat attgaaatct tgttaattga tgatggatct acagatggta gtagtgacat
     8641 tggtgacgaa attgctaatc tagagagtaa agtagcatgt tatcacttgg aaaacgctgg
     8701 tccatcagtt gctaggaatt gtgggataaa agaagcaact ggaaatttgg taggattttt
     8761 agatagtgac gattattttt tacctgattg ttttaataat gttttgaaag actaccatag
     8821 gaattacccc gatatcattg cttttggatt aagtaaagga aaaaatatag aaaatgcaaa
     8881 tttatttgtt cccgaaaaga agattggttg ttcaagtgaa gaagcaattg agcaaatgtt
     8941 tagaagtaag gcagtggatt tttacgtatg gaataaattt tttaaaaggg aattattcaa
     9001 tcatatagtt tttccagaaa ataaattata tgaggatatg gttcctatgt atgaagcttt
     9061 tagagcggct agtaaaattg atattttgcc attttcaggt attttttatt atcaaaataa
     9121 tgatagcatc gtttatcagt cttttaattc aaaacagtat gataatattt tgcaaagaaa
     9181 aatattagtt gatgaagtaa agaaagatta tcctcgactt attaatttgg ccaatgctcg
     9241 attagtagac ggataccttt caacagggtt taaaattact ggcaagaatc aaaatcagaa
     9301 agaaaagcaa aaatattata atcagtctaa aagagaaata aaaagtatat attcaaaaat
     9361 aagaagtaac aatgagatat caattgctaa aaaattagct ttatttttat acttaattaa
     9421 tcctaaaact tattttattt tatataaaaa agttctaaaa aaataaagtt tttttcgata
     9481 tgttattttt tatgtttatt aacttcggaa gactaaaatt gttactggta atgatacatt
     9541 tgtaaaaata aaaaagatga tttttaggaa gtgaagagag tgcataaaat tagtgttata
     9601 gtacccgcat ataacgtaga agactattta gaaaactgct tagaatctat ttccaaccag
     9661 acacattcaa atattgaaat aatagtagtc aatgatggtt ccccggatcg aagtggggaa
     9721 attgcagaag aatataaaaa gaaagatatg cgattaaaag tgatacatca agtgaatggt
     9781 ggtttaagtg cggctaggaa ttctggtctt gattctgcaa caggagattt tgtgtgcttt
     9841 attgacagcg atgattggat agaaccagac tatcttgagt tactatattt tggcacgcaa
     9901 acttatggag cagatatctc ggtgctaaaa ataaaaaaga tttcaagttt agaacaacta
     9961 aaagaacata caattgctga gagaaaatgg gaaatatttg atagagaaca agcaatgaaa
    10021 aacttattta taaataataa aattggctat tctgcaaata ataaattatt caatagaaat
    10081 ttatttgata atataagatt cccactgggt aaattaatgg aagataaggc tactacttat
    10141 ttgctgatcg atcattccga aaaagttgct gtcaattcat ctgctaaata tcactattac
    10201 caaagcccta aaagtatact gagaggaagc ttcaatccta gaatttttga ttcttttgaa
    10261 gtccatgaag atatactaat attcatagat aagttttatc cggaattatc aattaatgtt
    10321 agaactcgct atgtctatga agcaataagg atgatgatga caatgattaa aaataactat
    10381 accaataaag aagattattt acgttctata aatattttac aacgctttga aaaagaagtg
    10441 aaaattgatc ccgacttttc accaaaatta aaaaaattag taaagttttt attaataaat
    10501 aaaaacttgc cttactatat gtcaagtagt aagatagcaa gtaaattctt gattaagata
    10561 gaatctgtga ggtaagagat gaagaatata aaaaaat
//
LOCUS       DACEUZ010000095        10410 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.095, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000095 DACEUZ010000000
VERSION     DACEUZ010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10410
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.095"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(<1..630)
                     /locus_tag="IXG44_001994"
     CDS             complement(<1..630)
                     /locus_tag="IXG44_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HAQ1658703.1"
                     /translation="MIKEKIDSFVTIDDLQVGKPDPEGYLLCLKRLRVEQAVVVEDSP
                     VGAQAAPHMTVDVQALDADFFAFSGHKMCGPTGIGVLYGKRQLLEQMEPVEFGGEMID
                     FVNLYDSSWKELPWKFEAGTPNIAGAIDYLNEIGMENIHRYEQELVAYVLPKLQAIGG
                     ITIYGPQDPEHHTGVVAFNLDGIHPRDVATALDMEGVAVRSGHHCAQRFI"
     gene            complement(754..939)
                     /locus_tag="IXG44_001995"
     CDS             complement(754..939)
                     /locus_tag="IXG44_001995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1658704.1"
                     /translation="MTKALIFDFDGTMIDSEWMFYESIHHYLLKPIYWSSSAIKFLLQ
                     TINKVLGRQQNCPFMKG"
     gene            1166..2439
                     /locus_tag="IXG44_001996"
                     /pseudo
     CDS             1166..2439
                     /locus_tag="IXG44_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250512.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(2546..3577)
                     /locus_tag="IXG44_001997"
     CDS             complement(2546..3577)
                     /locus_tag="IXG44_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1658705.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(3584..4414)
                     /locus_tag="IXG44_001998"
     CDS             complement(3584..4414)
                     /locus_tag="IXG44_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1658706.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAKTGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            complement(4411..5217)
                     /locus_tag="IXG44_001999"
     CDS             complement(4411..5217)
                     /locus_tag="IXG44_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ1658707.1"
                     /translation="MKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLANYS
                     DIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLAANF
                     AGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYPSMK
                     EVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALTSSN
                     VNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            complement(5223..6047)
                     /locus_tag="IXG44_002000"
     CDS             complement(5223..6047)
                     /locus_tag="IXG44_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphatase"
                     /protein_id="HAQ1658708.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            complement(6037..7137)
                     /locus_tag="IXG44_002001"
     CDS             complement(6037..7137)
                     /locus_tag="IXG44_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ1658709.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCGSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            7315..8100
                     /locus_tag="IXG44_002002"
     CDS             7315..8100
                     /locus_tag="IXG44_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase"
                     /protein_id="HAQ1658710.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            8133..8516
                     /locus_tag="IXG44_002003"
     CDS             8133..8516
                     /locus_tag="IXG44_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAQ1658711.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            8592..8723
                     /locus_tag="IXG44_002004"
     CDS             8592..8723
                     /locus_tag="IXG44_002004"
                     /inference="COORDINATES: protein motif:HMM:NF012387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ1658712.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            8692..8928
                     /locus_tag="IXG44_002005"
     CDS             8692..8928
                     /locus_tag="IXG44_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ1658713.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            complement(9006..9977)
                     /locus_tag="IXG44_002006"
     CDS             complement(9006..9977)
                     /locus_tag="IXG44_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HAQ1658714.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
BASE COUNT         3076 a         2432 c         2002 g         2900 t
ORIGIN      
        1 gatgaaccgt tgtgcacaat ggtgtcctga acgtacagcg acaccttcca tatccagagc
       61 agtcgccaca tcacggggat ggatgccatc caaattaaaa gcaaccacgc cagtatgatg
      121 ttcaggatct tgcggtccgt aaatggtgat accgccgatc gcttgtaatt taggtaaaac
      181 gtaggcaacc agttcttgct cataacgatg aatattctcc ataccaatct cattcaagta
      241 atcgattgct cctgcaatgt tcggagtacc agcttcaaat ttccatggca gctctttcca
      301 tgaactatcg taaagattga caaaatctat catttcaccg ccgaattcga ctggctccat
      361 ttgttctaac aattgacgtt tgccatataa tacaccgatt cctgtcggcc cgcacatttt
      421 atgcccgcta aaggcgaaaa aatctgcatc caacgcttgt acatcaactg tcatatgcgg
      481 tgctgcttgg gcaccgactg gactatcctc aactacaaca gcctgttcaa ccctcagccg
      541 tttcaagcac aacaaataac cttctggatc tggtttgccg acctgaagat catcgatcgt
      601 cacaaaggaa tcaatttttt ccttaatcat cggatgtgct tcaaaaaaat agtctagctc
      661 ccgcagttca gaattgagac tactccgatt ttccagtgct gctgatccgc tgctcaaaat
      721 gttcagcatc cactggtctc ccaaccgctt gacttatccc ttcataaaag gacagttctg
      781 ctgccgtccc aacaccttgt tgatagtctg caaaaggaat tttatagccg aactgctcca
      841 gtaaataggc ttcagtaaat agtgatgaat actctcgtaa aacatccatt cactgtcaat
      901 catcgtccca tcaaaatcaa aaataagtgc tttggtcatc ttctttcccc cttacccaaa
      961 acaagcaaaa gctgtgtcct gctctctcta cagtatcagc ataaaaactg ccgtctgaaa
     1021 agtcaaaggt gaaagaaatg tggcgctata atttctagga ctgatagtaa atcattctat
     1081 cagtcctttt gagcataaaa aaacaaaaca tacagaagct ccgttagaat tgaatcacca
     1141 caacacaatc aaaggagcgt tctgtatgcc tgatcttcat cttatcaaac tattactagg
     1201 aatcaaagat ggaaacattc aaatcactca tgtcgagaag aaaaaaatac attcaatcaa
     1261 cagtatcgtt gtttctgcca cgctcattaa aacgattcag ccctgtcctc agtgtaaaca
     1321 ggtgaatcaa catgggctga tcgttaagaa cggaaagaaa aaatcgctgg tccttctcaa
     1381 taagtgtgcg aatcaacgga cttatcttgc tttggcaaag caacggtacc tctgtcgtgg
     1441 ctgtcacacc tattttacgg cgaataccta cattgttgaa aaaaactgtt ttatcgccaa
     1501 acaagtacat tataaaatat tggaggaatt ggctgaaaaa caagcaatga cgacgatttc
     1561 taaacattgc ggcgtttctt ggtcaaccgt ttcgcgaaca ctcgcttctc tgctccccat
     1621 gacaaacgtg aaaataaatt ggttgcctcg ctgttcattg gtagatgaat ttcgttcatt
     1681 gaaaaatcaa gtcggtccct attctttttc ttgtatggat ggcgatactg ggaaattact
     1741 ggatattctt ccttcacgta agaagaaaga tctcatttct tattttatgc aatttgaacg
     1801 acgtgcacgt ttaaacgtaa aaatactcgt aacagatatg aacgcctctt atgctttgct
     1861 gataaaagag tgctttcctc atgccaaact cgttgtggat cgctttcaca tcgtcaagca
     1921 cctcattcgc agttttaagg atatttgact atgagtcatg aaaagcttcg accgaaacga
     1981 tccaatacaa gccaaacact atcgacaagt gaaggcttta tctcgacttc tgatcaaacg
     2041 acaggacacg cttgtttatg ataaatggac aaaatggcgt aattttggtt gggcctattt
     2101 aaccgaaagt gaagtcgtag aacgtctact ttctacttct gatgaactac gaatagctta
     2161 tgcttactat caagaaattc ttcaagcttt ttatgacaaa gaagcggaca tcttctttca
     2221 gcttgtaaaa acaatgccca agtcggttcc aagagaactg catcatataa aaaaagcgtt
     2281 tataaactat gaatccggca ttcgtttagc cttagagttg ccttactcaa atggcaaaat
     2341 agaaaatctg catacccaca tcaaagcatt gaaacgaata gcttacgggt taggatgaga
     2401 accagaattt tcttaatcaa tcatctgatc acttattaaa cataaaaaaa catctaaagc
     2461 agaaaatcaa ttctacctta gatgttctta tatcactcta tcagtcctat ttgacaaaga
     2521 gccagaaatg tttgccgact atacactagc tttgttgtgc aaaatgctga gtatcaattt
     2581 ccacagggat tgctagaata tctttcagct gaatgtataa ggcatcgcta attttctctt
     2641 cccgatcgtt caaccgatcc actaaaattt ctttgccctc cagcgtttga aaaacatacc
     2701 gttgaatgtt gcctaaaata aatgattcct tccatagaac cggaatctta accccctgag
     2761 gaaccggttc aaattgaatc agctccggtc ttaaataata aatgcagcga ttgcccttag
     2821 cggaaaactc gtgggtatac tgattcagct cataccattc gaaacgatta tagttgccga
     2881 taaattccgc aacaaatcgg ctctgaggat tctgatacaa ttccttcgag gtggaaacct
     2941 gagccacccg cccagcttcc atcacaaaaa tccgatcact gattcgcatc gcctcttctt
     3001 ggtcatgagt cacaaaaatc atcgtcatcc ctagcgattg ctggatagcg cgcaaatccc
     3061 gctgcagctg ttttctaatt cgggcatcca aagcacttaa tggctcatcc agcagcagga
     3121 ctttcggttc agtgaccaat gagcgagcca gtgccacccg ttgtcgttga ccgccagaaa
     3181 gaaactggac atttttatct gcctgctccg tcatatccac caatgatagc atctcaaaaa
     3241 ccttttcctg aatcacctca ggactcagct tctttacctt caaaccaaag gctacatttt
     3301 caaaaacagt catagttgga aacaatgcat actgttgaaa aacaaagccg atattgcgat
     3361 ccttcgtagg cttatcggca atattctcat tgtccagcac aatctcaccg ctctttggtt
     3421 gttccaatcc cgtgatacag cgcagcaacg tagactttcc gcacccgctg ggacctagta
     3481 aagtaaccag ctcacccttt tcaatctgaa tttcatcaat agctaaaatc tgtttttcat
     3541 caaaaaacag attgagatca tgtatttcta aaaacatttt ccgtcacaac tcctttaagg
     3601 gcttcttttt cgactgcttc tcttccctcg tctgcttaag ggacagctgt tgcttttgtc
     3661 gataagtcag cataaaggta gttaaagcaa ttcccaccat aaacagcaaa tacaaaacca
     3721 tcacagcgct agccaaatgg ccgttttcgt tcatccgccg cagcatatag atgcgtaccg
     3781 tctgaaatcg accgccaatc aacaggttcg tcagcatgta ctccccaaaa accgaagaaa
     3841 aaatcatcag agaaactagg gtcacaccga tacggatgtt tggaaacagc acctggataa
     3901 taatcgtaga catcggggcg cccaaggttt ctgctgcatc cactaattcc ttcagattaa
     3961 ttaagcgcat ctggttgtta agactaagat agacaatcgg caacgccgta ataaacaatg
     4021 cacctaccaa cactacaaac attgggatac cagttttagc atacgttcga agcaaagccg
     4081 ttaccaaaat cactcctggt aaagcatacg gcagcaggag cagcttttcc agccaacgat
     4141 tcaaacgagg gaaatacaaa tgaatccaaa taatggtggg aatcatcaac aacagaatcg
     4201 tcaccaagac cacacctgct aataaaagtg agcgacccac cgcagcaata aattccctgt
     4261 ccgtcagcag ctgttggaac cattgcaagg taaaatcact aggtaaaagc gattttgacc
     4321 atttagtgga gctggcatac aaaatcgttc caataatcgg cagcaagtaa tacaggacaa
     4381 agcagaacaa aatcggtttt attaactttt tcacgtctgc accaccttgc ctttcttttg
     4441 aataatcaac attgccgtta ccagcaggac acctaaagcg actgccaacg cactgcctaa
     4501 attaggcttg gcgaacacat ctcccgacac taaagccgca attcgaatcg ttaaaagatt
     4561 tacattactg ctggtcaggg catacgctgt ttcataggtc cccatgccat tagcaaataa
     4621 gatgacaaag gtgctggcaa tcgatggtcg caaaaaaggc aaagccacct tgaaccaagc
     4681 atacgctgat gaagcgccca gcaattgtgc ggactccagc cattcctttt tcacttcctt
     4741 catgctggga tacaaaaaca agacccctaa cggaatttgg aaataggtat acgtgatggt
     4801 caatccccac caagaataca aattgaagtc cgctagaaat ggcaaaatca ttttcagcac
     4861 acctacattc cccaacaaaa taataaaaga gaatgccagc ggaatcccag caaaattagc
     4921 tgccaaattg gaaacaaagg tgattttctc ctgcaattta gtcgatagat tcattaagca
     4981 taaacataaa ataaacgtcc cgattaaccc aatcacactg gagctgacag caatcagtac
     5041 gctgttaaca aaggcctgtg tgtaatattt gttgctgaaa atatcactgt aattggccaa
     5101 tgtccactgg ttagttaaat cactgtgcaa gctgcacaga accataaaaa gcagcggcag
     5161 cagtaaaaat gcacagaaga taaaggaggc aggaaccaga accaagcgtt ttttcatctc
     5221 tttcatcccc tcatcaattc ttgaatgtcc tgcattttct ctgacggtgc aattcctaat
     5281 aaccagcaaa acaatggagc cgccagcagc tgtggtacag tttgttcctt cagcttttgg
     5341 cgtttatttg aaatcaaata aaaaggaact tcacgatgtg cagccaagct gccgccatgc
     5401 agtccgtgcg catccatccc gtgatccgca gtcaccacga cttgataacc tttttgcagc
     5461 cattcaggaa ggtaaatagc caataatctg tccgcctgat taaccgctgc tgcatattca
     5521 tgagaatcgg cggtaaattt gtgaccgaca tcatcaatat tcatcgaatg tacgaccaaa
     5581 aagtctggct gatactgatt cactaaataa tgggcatccg caaataaatg cgaatcaggg
     5641 taagtgtcct cactataaaa gatgccatgc tgaatcagtc gctcctcctg atgctggatg
     5701 cgatcaacca aacgattata gggggcttga ttgtacaact cgctgtacca gtggtaagcg
     5761 gcggctcctg tttttccgcc agcttcctta actaattcaa acagcgacca ctctttggat
     5821 cgctgagcat ggtaattact atagatgcca ttttcatagg ctggaacgcc ggtcatcagt
     5881 acttcgtata acggacgtga attgctgggc agctcacttt ttaccttcaa taagcttgct
     5941 tgctggtgct caactaaatg attcatgaac ccaagctggg cgacggctgt gtcgtagcga
     6001 cagccgtcta gtataactac aatcagtttg tcaagtctac tttgcatagg tcagtacctc
     6061 cgcctgccaa agctctggaa gctctacaga ggtcttgttc caagcttcat tgtcttcaac
     6121 tggcaccgcc gcttcataag catcctccgg cagtaatttt tcagctactt ctttaggcag
     6181 ctctaccttg cgaataggtc gcgcatagcc ctttgccaaa ttgatttgcc cttcatcaga
     6241 taaaatatat tcccgtgcta gctttgccgc attgggatga ggggcatttt tattgattaa
     6301 ggttgtatac ccgctaatga tggagccgtc tgttggcaca gttacctcaa aacgctcgga
     6361 gtcaatttgg tcacgataat ttagtccatt gaagtcccaa acaactgcca catcgatttc
     6421 acctttttca agatttgcta cgctggcatc aacggtggac aaacggcctt tttctgccat
     6481 ttttttgtag taattcagac ctggctgaat atccttttcg tcacctccgt tggcaatcgc
     6541 cgcagccaat acagcaaatt gtgcttggtt ggcaatcgtc acatccccca cgctaacttg
     6601 atagtcaccc tcttgcagct ccttccagct agtcggtgct tgcttcacat tctctttatc
     6661 ggtgataaag gaaatggttc ccgtatagct taacaaccaa tccccatcat catcctttgc
     6721 ccaatccgga atttcctccc aatagttggt cttataaggt aaggttaact ctttgctttc
     6781 agccaacgga ccgaagctga taccgacatc accaatatcg gcggttccat tttttccttc
     6841 tgattcaaac ttagccaatt cttctgcact ggacatatcc gtatccacgt gacttaagcc
     6901 gtattctttt tcaaggtcgt tccaagtgcc tacccaattt gcccaagtat ccggcattcc
     6961 caagctggca atctctcctt cttccttggc tttttctata atcgtatcta aagagtcgtc
     7021 tgccgcagcc gattgcttcg ttgcttcacc tgagccgcag cctgtcagca ggatacttac
     7081 tgttgctgca caaaataaac cgatagtcat gttacgaatt ttttcattca ttttcattca
     7141 ttcttctccc ctttcgataa ttccattcta acaaagcttt gtaatgagag aatcagtttc
     7201 ttgaaaaggt ttcatgaaca atgtaaaggt tgtatatggt ttgtaaattt cgacgaattc
     7261 ccgttaacct tctaaagctt ttgcggtatg atagtagaaa aaaggaggct ccctatgcag
     7321 tattatgatc agcatctcca cacgtatttt tcctttgatt cagaggaaaa atttgaaaac
     7381 tatttagcct atcagccgga gtttttcgtt tctacggatc actttgattt aaacaatcca
     7441 tctaccggaa aagatgacat tccggattat ccagcctata taaacaaact ggactcttta
     7501 aaagaaacct attccaccaa atttctgcga ggtatcgaga ttggcgttgt tccaggacaa
     7561 gaagagcgga tcgtggatta cctcgctcag catccttatg atttaaagct ggtaagtatt
     7621 catcaaaatg gccgctttga ttatatggac gatgtggttt taaccaaaga tcccattcaa
     7681 actaccaagg aatattttga tcagatgttg caggtcttga cggattttcc agaaggagat
     7741 attttgactc actttgatta tggattacgc cgcttcacct tcacagcgga agaattcgcg
     7801 gagcattttg aggctcagct gctaacgatt ttcaaaaagg ttgttgaacg agaattggcg
     7861 atggagttaa atgccaagag cttcttgaaa tacggcaacg aagaccttta ccgttatgcc
     7921 gttcctttgt atcaaagtgt cggcggaaag ctctttacct taggctcgga tgctcatgtt
     7981 gccgaagatt atcagctggg ctttaaaaaa atgagtcagc tgttggcaga ctgtcaggta
     8041 gaaaagttgc tagtcattca aggaaaagaa cgccgcctga cgccgctgcc gattttgtga
     8101 ttcctatata atagaagaaa gtggtgaaaa aaatgagcaa cctgtgggac tacaatcaag
     8161 aggcacccat ccattattta attgcgcgac attgggatgc gttgaaaatt gaggcggttt
     8221 gccgatcctt gttagcagcc gttccaaaac aacaactgga aaattttttg gtggctgaca
     8281 gcttgcagcg ggaaaaagtt caggcttatt ttgcagcctt taaagatcag cctttggaat
     8341 atttgcatgc gcagtttcat cttttttatc aagtagccgc cccagatgac tacaacgatt
     8401 tacgaggaca gctgcaattg acctttcaag cggatgaaac cgcctatacc gttttattgg
     8461 gaatggctcg ccttggtgat caggcgaagg tcgagtggcg gattttcgat atttgatggc
     8521 aggatcaagg cgtttcagtt gcaaaccttc ctctctttca attagaaaaa ccgcagcagg
     8581 aagaagtttg tgtgctggtc accgatgctg gtgaaattcg tctccgctta tttcctgacc
     8641 aagctcccct ggctgtagcc aactggcgct ccctagctga gcaaggcgtt tatgacaaca
     8701 cacctttcgg ctcgtgtcat taaggaattt gttattcaag gtggcgcttt ggatggttct
     8761 ggtgatgaag ctgtttccag ctatgagggc tttttcgctg atgaagtgca tcgcggactt
     8821 tatcatttta atggcgcctt ggccttaggg gaccacggcc cccacaccaa cggcaaccag
     8881 ttttttattg tgcaaaacac aaaggcgcag gctgacctgc taatgtaaag caagcatact
     8941 tggagcttgg aggactaccg gagctggatg gtcgttatac tgtttttgga caggtatacc
     9001 aagggttaga aatcgtagca gcgatcgcag ctcagccgac aaacagtgaa gatgtaccga
     9061 ttgagccagt gttcattcga caaattaact tcgaaaaaac atcgtaaaaa gacactcact
     9121 gacttcagca aaagctctat tcttgagctg gctgcagcta gtgagtgtct ttcatttatc
     9181 cataagataa agcttggatt caataaataa ataaatgttt tggttaaagc tgtgaccagc
     9241 aaatgaggaa ccgtgcgagc cagttgatct gccaatgcaa acatttcttt aggtgaaggc
     9301 gctggcgtgt ctgccaacgg atactccagt cccaatgctt ggtaaccgtg cataccatag
     9361 cagtggtacg gtagtaattc taacggtacg aaccgatcaa gatctgccac aaaacaagca
     9421 atcgtcagca gctcggtcag gttcatattg atacctggaa ccacaggaac tcgaattcgc
     9481 acttcgcagt taggatactc aattaaaaag cgacgaatat tcttcaaaat ttgttggtta
     9541 tctaagccgg tccattgttt gtggagcttg ctggtaaaca tttttacatc cacaaacaat
     9601 gtatccacat agggagctac cttttggatt tcattataag caccacaaaa gctggtttcc
     9661 agtacggtat tgattccaat gtatttcgat ttttgcaaaa tttctttagc aaaatccgcc
     9721 tgaaccagtg cctcgcctcc gctaatggtc actccgccat ccgagtgaaa gtaaaaaaca
     9781 gcatccttct caatctcatc cattacctct tcagcggtca tggtctggcc aaatcctttt
     9841 tcaatctgct tgctttgtga ttcgggaatc gaacaccagg cacagcgtaa tgggcagccc
     9901 tttaaataaa caacggtccg taaaccagca ccatcatgta gagaaccctg ttctattctt
     9961 aatactgttc cttccatata gatttttgaa ttacacacta gtttaaacta aaacacacaa
    10021 ggaaaaactt accatttaat cataaaatta gctcatataa atatactagg atgtaaaata
    10081 gactggtcaa aatctggtca aaaaagagga ctaatacagg tagtcctctt ttttgttatt
    10141 ttaatattct aaatcatttt ttattggttt attcccttat tcgatgtaga ctcaaatcac
    10201 tatatcacta gatatcaaac ggcgtcatac ggaatcaaaa acatcaaatt tgacctgtta
    10261 aatggtcatt gaataacatt caaaatcaat aaaacggagt cagacacgga gtcaaaaacg
    10321 ctgagagtaa aaaatatata cagccttgta ggtaaagtac cacagggctg tatatttttt
    10381 atttcagatt accttaaatg attccattta
//
LOCUS       DACEUZ010000096        10398 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.096, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000096 DACEUZ010000000
VERSION     DACEUZ010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.096"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(126..878)
                     /gene="xth"
                     /locus_tag="IXG44_002007"
     CDS             complement(126..878)
                     /gene="xth"
                     /locus_tag="IXG44_002007"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HAQ1658715.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            991..1911
                     /locus_tag="IXG44_002008"
     CDS             991..1911
                     /locus_tag="IXG44_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ1658716.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            1984..2919
                     /gene="murB"
                     /locus_tag="IXG44_002009"
     CDS             1984..2919
                     /gene="murB"
                     /locus_tag="IXG44_002009"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HAQ1658717.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            3217..4695
                     /locus_tag="IXG44_002010"
     CDS             3217..4695
                     /locus_tag="IXG44_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ1658718.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            4972..7032
                     /locus_tag="IXG44_002011"
     CDS             4972..7032
                     /locus_tag="IXG44_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1658719.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            7115..7684
                     /locus_tag="IXG44_002012"
     CDS             7115..7684
                     /locus_tag="IXG44_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1658720.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(7859..8665)
                     /locus_tag="IXG44_002013"
     CDS             complement(7859..8665)
                     /locus_tag="IXG44_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1658721.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            8803..10317
                     /locus_tag="IXG44_002014"
     CDS             8803..10317
                     /locus_tag="IXG44_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HAQ1658722.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
BASE COUNT         3689 a         1735 c         2047 g         2927 t
ORIGIN      
        1 ctctcgataa acagtgttca aaagccagtt tctcggtatt atgccaaatt tatgcaaaat
       61 gtggaaagcc attttcacaa aatttttctt aatactcgaa actaaacgac tttttcacaa
      121 cctctttatt tcaaatctaa ttctactgga caatgatcac tgcccataat gtccgtatgg
      181 atctttgcat cagctagtcc tggctctaaa tctttgctcg tcaaaaagta atcgatccgc
      241 caaccagcat tgtttttcct tgaattgaat cggtagttcc accaagagta gacaccttcc
      301 tgggttggat aaaaataacg gaatgtatcg ataaacccgt tatccaacag tcgagaaaga
      361 gccgctcttt cttctggagt gaatccagca tttttttgat tcgttttcca gtttttcaaa
      421 tcaatctttt gatgagcgac atttagatcc ccacacacga tgactggttt ttgttttttt
      481 agattttcca aataattata aaaggcttct tcccattcta atcggtaatc taaacgtttt
      541 aattcgcttt gagaatttgg cgtgtaacag gttacaagaa agaaatcgga atattccaaa
      601 gtaatcagac ggccttctgt atcatggata tcgataccca ttccgtaagt ggcattcaat
      661 gctggttttt tagcaaaaat cgctgtgccg gaatagcctt ttcgttccgc gtaattccaa
      721 tattgataat aacctggaag atctaaatca atttgtcctg cttggagttt cgtttcctgc
      781 agacagaaaa aatcggcgtc taactcatga aaaacttcta aaaaattttt atttacgatg
      841 gcacgaagcc cgtttacgtt ccaagagata aatttcaata aaagcacact ccttcgaatt
      901 aatttcctta cctttgcgat actattgtac ctcattttag taaaaaatgg tgtaagatga
      961 aagtaactaa atgatccagg aggaaagtag atgaagattg gtgtaatcgg tgtagggaat
     1021 atcgcagaaa aagcatattt accaacttat gcgaaaaaac aaggaacact tgatttttat
     1081 tttgcaacaa gaaataaaga aacaaaagaa agaattcaaa aagagtacgg gttccaacat
     1141 ctatatgaaa cgatcgatga gcttttagaa gaaaaaatcg aagcatgtat gatccacgca
     1201 gcaacaaaag tacattatga attagccaaa aaatgtctag aaaatggtat ccatgtttat
     1261 attgacaaac cactgagtgt ccatctaaat gaagttcgtg aattgcaaga attagctgat
     1321 aagaaccaaa taattttgat gatcggtttc aaccggcgct ttgctcctat ggtagaagaa
     1381 ttgaaacgta tcccagataa gcggttgatc cacctgcaaa aaaatcgaat agcggcaaaa
     1441 gaaacgacag agttcgtcct ttatgatttg tttttacatc ttattgatac agctgtgtat
     1501 ttattagatg aacccatttt gagaacaaaa taccagataa gagaaacaaa ggaatttttg
     1561 gaatatgcca ttttacaatt agaaacacaa aatcaaacgg ctattttaac gatggattta
     1621 gcaagtggcg ctaatataga aaaatatcaa gtaacaagta aaaatggtac ttatgaagta
     1681 aacgatctga cccagcttgt cattcaagat gctgatggaa aaaaagtgaa agaatttggc
     1741 gactggacaa acacgctagt caaacgcggg tttgagccaa tggtagaggc cttcttttct
     1801 cagatccgtt taggtaaatc ggaaacagaa ttgcttaaac aaaaagaagt tgtaagaagt
     1861 catgaattat gtgaggaagt gcttaaagat cactaccgtc atcaactttg aacaagactg
     1921 gcaagattgt attaagcgtg atagaataat gaggacgttt gagaaaagaa aggaaaatcc
     1981 tcagtgaata aagaagaaat agttgaaagc ttagccacga ttactatatt gtttgatgaa
     2041 ccattgatga actatacgtt tacgaaaaca ggtggaccag ctgatgtcct tgcattcccg
     2101 aaaaaacaag aagaagtaaa gcaaatcatc gattattgcc gaatccatga tataccttgg
     2161 atggtattag gaaatgcaag caatctgatc gttcaagatg gcgggattcg cggtgtagtc
     2221 atcatgctga ctgaaatgaa acagattcat gtcaaaggaa cgatggtcat tgcggaagca
     2281 ggtgcctcac tgattgatac gacttatgca gctttggcag aatcattgac tggatttgaa
     2341 tttgcttgcg gcatcccggg aagtgtcggt ggagcggttt atatgaatgc aggtgcgtat
     2401 ggcggagaga tcaaagatgt ttttgctgaa gtggatcttt tgcttgaaga tggaacattg
     2461 aaaactttga caaaagacga aatggtattt tcttatcgtc acagtaaagt ccaagaatta
     2521 cgtgctatcg tattggaagc tcgattcagt ttgcaaacag gggattatga agcaatcaaa
     2581 gcaagaatgg atgagttgac cgaattgcgt caatcaaaac agccattaga atacccttca
     2641 tgcggaagtg tctttaagcg cccagtagga cactatacag gtcagctgat ccaacaagca
     2701 ggattgcaag gcctgaaatg gggcggtgca caagtctctg aaaaacatgc tggattcatc
     2761 gtcaatatcg atcatgcgac agcgacagat tatattgaat tgattgcgca catccaagaa
     2821 gtaatcaaag aaaaatttga tgtgtcttta gaaacagaag tgcggatcat tggagaaaaa
     2881 caagcaatat cagaaacaag cagaagttta gccaattaat aagtaatgga acttaaaata
     2941 agtaaaagag caggacggtg gccgtcctgc tcttttttgt ggttgtttac gcaaatggcg
     3001 caaatcttgt acattttaac acaataaaac aaaaaatata gaatatttcc tcataaattt
     3061 ttccattttt tgcaatattc tcaaaaaaaa gataaaaaat gagaataact gatgaaaaaa
     3121 caagcataat ccatcataaa atcagtatag gtgaaaagat gaactttcat ctgcatatca
     3181 agtaattggt atgaggcatt ttttgaggtg taggatatga agttggatta ccttatagaa
     3241 gaggaagata gagaaaaact gctgctagct agatatttag aatttgagaa aaagaattat
     3301 gtagtgctgc ctaagttata tgaaacatta ggtttgtcta aatttaaaac aagaaattat
     3361 atcatagaat taaataaaga atttactgaa ttcaaaacaa agccagagat ccaaatttca
     3421 gaagaagcag agatccatgt cgaacgtctg aatttgacgg atatcaaaaa tttaagaaga
     3481 atctactttc aacgatcaaa aattgctcga ctgttaacgg agatcgtgga ttcaaaagta
     3541 acgatcgaaa aatttgcaga gaatcatttt ttaagcatat caagagccta tgtaaaaaga
     3601 agagaattag tctcttttct tgaacagcat caaataaagt taaagaaaaa catattagtt
     3661 ggggaagaaa taaacatacg gaacgtatta tttggggtat atttcggggt ctttaatggt
     3721 ttttctatgc catttagaga aagcgtcatg aatgaagcaa agagtatcgt gcaatatttt
     3781 gaatatctgt tcaatttaaa actgtctgca acaaaacgaa tcaaattcaa tttgctaacc
     3841 actattagtt tgttgagggc ctatgggaat caaccaatcg gaaaacgttt cttttcagat
     3901 gaatacttta agagctcaga aggaatgaag atcatcaagg atttatcaca tatttctttt
     3961 cttcatatcc ctaaagagca gatcattgat gagatttctt atatcatgtt gtttttgatc
     4021 acggaagaag aatttgaatt agatggcgca tttggacttg cggaagctca ttttcatgat
     4081 atccacatga ttagtcaaaa aatgtcagtg gaattgattg atcaattacc attttcgatt
     4141 gaaatctctg aagaagaaag actatatata cgaaaagaac ttcgtatggg tatgatgcat
     4201 gtcaatcgaa aaaattggat attcgacttt gagatgacta gctttacgtc taaaaagcag
     4261 ctacagtttt tcttcgagac gtatccaata ttcagtacag caatccaaaa ggtcatacaa
     4321 gagaataaac gctatttctt gggaaaagat gaacgtttca tgattcgctt attttatgat
     4381 tatctctttt tattggtcgg tatttgtccg gtatcatatt ttgaagcacc gattcatgtc
     4441 tgtattgatt tttcgcaagg atctgcttat accgattaca ttatttctca agtgaaagga
     4501 tttaaaaatt acaatattgt gattgagagt cgaatcactt ctcgtacgaa tatttattta
     4561 tctgattgtt tacttgaaaa atttaataaa gaacagataa tttggaaaaa tccaccgact
     4621 ccagatgact gggaatattt tggaaatacc atcatcagag aaaaaaatag acatttaact
     4681 gaaaaaatca gctagtattt atggatggca gctttgtctc acatatttat tagctgattt
     4741 atgttttttt caaagaaaag gtaactagat ataactagat ataactagat atattactta
     4801 acaaaattaa aaagtacata agttattatt ccgattttct tgaaacaata aaaaatcaaa
     4861 aaaaatactc tcctaatagt ccatcttctt ttgattttat ttatataatg aaatcatctt
     4921 atgaaaatta ctacttcatg tttagacaag agtagggagg actaagaata aatgtgttca
     4981 aaaagacgtt caaaacaact aaataaacac gcttgtcgtt tagataagga acggtcgtgt
     5041 tctgaaaaac actacgttaa gtttatgatg cgcacgatgc tgatcagttt atctgtaggt
     5101 gtctatatgc atgtacccgt tcaggcaacg acacaaacca tcaaggtcgt acgtacctct
     5161 gtttctgaga ccactgattc ccctatcact attactcata ctttcactga tgcaaatgga
     5221 aaacagctac ttcctgcatt gacttcctat aataaagtca gttatcctcc aacgataaac
     5281 cactactggt taattcctaa caaaacatta tggacatcgg aaaccggaag cagtgtgccg
     5341 ctttctatct tttccgatat gactgatcct acaaaaggtg atcagttaaa acagatcact
     5401 gatattttga atcatgcttt gacagatata aaggacggtg gaagcgcaca agtttcttat
     5461 gtatatgaaa aagattattc acagtttgaa agaaagaaaa tcgatgttct ggcagggaca
     5521 aaagtttcga tatctgatct ggtagaaagg ctgaagaacg cagatggaac acaaggagat
     5581 atgtccaaag taatttcaaa caatgggaat atcgtggata cgactaatac aggaacatct
     5641 actatttcct tgtcctattt tgatccaatt gctttaaaaa caatgactgc atctgctgtg
     5701 atccatgttc gtgctgatca aacagctatc aaaggaaaag atcttcactt aagaacagga
     5761 gagagttacg cccctaaaga tcttgttgaa tatgtaacaa aaagcgatgg aacttcagga
     5821 aataaagaag aaatcaaaat gaacgggaca gtagatacta caactccggg agaatatccc
     5881 gttgtattaa cttatataga ctcgaaaaca tcaaatgtgg cagaaacaac agctgtagtt
     5941 tatgtagaag catctgctgc ttcatcgact gtcatcactc attttataga tagtcaaaca
     6001 aataaagaac ttcaagcaga aacacatatg agtagtgtaa atcgtattcc tgatttgcgc
     6061 ctacctgctg aattttctga tcgagtgcca aatatagatt tatcgactta tgaacaaaat
     6121 gggaaagtca cttctatttc tgattggatg caagcaaacg gattagacag aaaaagtcac
     6181 tggtcggaga tccttgaagt acttcactca gaactgatga ataataggaa gcaatcagga
     6241 ggggaaatta cctttactta tgtttatata ccagacaatc gttcattaga aggtcatgat
     6301 cttattgttc atccaaacga aaaaataaac aaagaagagt tgatccaatc agcaacaaat
     6361 tccttaggta aacccgtacc gttaaaggat gtatcggtta caatcaatga agtaccattg
     6421 gaggatggtt atttgaccaa acaaacaggt gattttacga ttatgtatag ttatcatgac
     6481 ccgtattcac tccaagaact ccatgctcag tcactattac atgttttacc attaaacaaa
     6541 cagatagatc aaaaagaaca aaaaagacaa gaagaaagct tgtcagaaaa aaaacaacaa
     6601 atggctcatg cagtttctcc aacagggaaa gtaaaacaaa caagaagcct agagttagca
     6661 agaatatcta gaaaaatctc agaatctaat aaaacgataa ggcaagaagc tcatttggcc
     6721 ccacaagttt ctggattgga aaatgaaatt ttagagccta aaataaaaca acaatcagaa
     6781 aaagaatttg ttgctaaaca gtcggaagat cagaaggaat cagatgacca gaaggaaacg
     6841 aagaaaaaac gcttgtcaac tattgattcc accgatgagc atctgcagcc gcaagtacca
     6901 cttcctagtc cgggaacacc cgcacctggt ggaggtattc cttcttctgc tttaggagat
     6961 ttcatgcgag gtatggcagg aacatgggta tacgctaatc gaaatgaatt tgacgcagat
     7021 gactggttct gatttatatc tctatctgtt atagtcaaat aagtagctga gtcatatgtt
     7081 atttggcata atggagacga ggagggatag aaaaatgtta gataaaacgt taccttacgc
     7141 agaaatctgg atgacaagac cacttcaaca aaatgttcca atcacgcctt tagcggaagg
     7201 ttttgaattt cgattttatc aaaaagaaga tgaatctgct tgggcggaga ttgagacttc
     7261 cgttggggaa tttcaaacga cagatgaagc acttgagtat tttcaacaaa actttgctcc
     7321 ttatcctgaa gaacttgaaa gaaggatgtt ttttgtagag acaaaaaaag gagaaaaagt
     7381 agcaacctgt accgcttggc gaaaaaaatc aagagagcaa gtgtatccgg tttttcattg
     7441 gttagcagtg aaaccagatt accaaggaaa agggctcgca aaagcattga ctgccagagt
     7501 cttgcaagaa tttcctaccc tccatacgga cggaccaatc tatcttcaca cacagacatg
     7561 gagccatcag gcgattgctc tttataaaaa aatgggtttt acttttattg ctgaaaatct
     7621 agacggcaca cctaatccag attatgaaaa agttatgcag gttttaagta aattagataa
     7681 ataacgaaat ggtgaaataa gtcctcagta ttgggataaa aaacgaaata gtcattcctc
     7741 atatcttact tttttgagtt aatactatcg gataactaat ggcatgccat acattcaaaa
     7801 aagaaggggc agtgacaaaa gtattgtcac tgccccttct cttgtttttt agcatgtttt
     7861 atcgtttgat ttttttagga ataaaattct tgatgaacgt aaatttttgg taatcgtttc
     7921 gactatagag gtaaccaaat acactgaaaa caactgctcc aataaaatta acaaataaat
     7981 ccttcatagt atccatgatt cccacgtcta agtaaccatt ttggatgatt gttttatctg
     8041 ttttaccatc tgcattttga ctttcaatga ttgtccgagt gatatctgga acatgataga
     8101 cagtgtttcc gtctttactt aactccacac tattgatttg ctggatgatc gtatcttttt
     8161 gcatatcgaa ttgcagtaat ttatctgctg aaaattccac aaactcccac ataacaccaa
     8221 ctgtcatcga aaaacagaag gaaacaactg ctagtaataa aggagtcaag ttcatggttt
     8281 ttgtattttt attgagcaaa taaacaaggg aaaagccgac acccccagct aaaaatccgc
     8341 tgaatgcatg tagaaaagta tcccacattg aaaaatagcc ataaaaatca cttaactctc
     8401 ctaaaactgt tgaactaaaa ataaaagata acaaaatgat ttctagcata tttggaatct
     8461 gtatctttaa tatcttttcc agaaaataag ggatttcaaa taaaattaat gtcagtaata
     8521 aaagcattgt atttccccag tgttggttcc agacctgatc cactagcata ataacgacga
     8581 tgatacgtaa taaaatatag attccgtttg tgattctacc tgcagttttt gtactgttcg
     8641 taaaaaaggt tttcaaatga ttcataaaat aactccttca tgtatatcct taattatact
     8701 aagaagcgtg tgtttaccaa agctatatga cttaaagaaa aacagagttg atattgtgaa
     8761 aaaaatcact taatatagta taaggagaaa agggggatac aaatgaaaaa attagttgct
     8821 ggtttattaa cagccgttgc acttcttgct atgagcgcat gcggcgcaga tcagaaaaat
     8881 acagcgcaga caagttctag tacgaaggaa tctgccgaag tatcatcagg cgcttctgaa
     8941 caagtatata cagatccaaa agaaatgaaa gaatcttatg atgtcgtgat tattggagct
     9001 ggtggtgcag ggatggcagc agctatcgaa gcaaaagatg ctgggttgaa tccagtaatt
     9061 ttggaaaaga tgcctgttgc cggaggaaac acatctaaat catcaagcgg tatgaatgct
     9121 tcagaaacaa aatttcaaaa agaagaagga atcaaagact cgaatgatgc cttctatgaa
     9181 gaaacgctaa aaggtggtgg aggaacgaat gataaagaaa tgcttcgata ctttgtcgat
     9241 cactctgctg atgcggtcga ttggctagat caaaacggta tcaaactgga caacttaacg
     9301 atcactggag ggatgagtga aaagaggaca catcgtccgt ctgatggctc ggctattggt
     9361 ggatacttag tggaggggtt attgaataac gtccatgagc gggaaatccc tatttttgtc
     9421 aacgcagatg tgacagacat taagaaaaat ggggaaacgg tcaatgaagt gactgtacat
     9481 gttcaaggag aagaaccaaa agaggtcacc ggaaaagcaa tcgtagttac cacaggcggt
     9541 tttggtgcca gcaaagaatt tattgaaaaa tatcgtcccg atttagctga ttatattacg
     9601 acaaatcaag aaggatcaac tggagatggt atcaccatga ttgaaaaatt aggtggacaa
     9661 accgttgata tggataaaat acaaattcat ccaactgtgc agcaggatga gggggtcttg
     9721 attggtgagg ttgttcgcgg agaaggagca atcttggtcg atcaagaggg gaaaagattt
     9781 gtcaatgaaa tggatacaag ggataaagta tctgcagcaa ttacagcatt gcctgaaaaa
     9841 tcagcttatt tgatttttga ccaaggcgtg cgtgatcgtg ccacagctat tgaattttat
     9901 gaccaaaaag gatatgtcac aactggaaag acaatggaag aattagcaga aaaaatcgat
     9961 ttaccaaaag aaacactagc aaaaacagtt gatggatgga atacggatgt tgcggagaaa
    10021 aaagatgctc aattcaaccg tgccacagcc atggaacatg acttatcgtc tccgaattat
    10081 tatgcgatca aaatcgcacc aggaatccat tacacgatgg gcggtgtgaa aatcaatacg
    10141 aacactgaag tattagataa agaaaaccag ccaattaggg ggttatatgc tgctggtgaa
    10201 gtcacaggtg gcttacatgg aaacaaccgg atcggtggaa attcagttgc ggagatcatt
    10261 atttttgggc gacaagcggg catacaagca gctcgatttg cttcggaaaa tgaatgaaat
    10321 agaacgtaaa taaaaagagg ttgtgaaatc agcgttttga gctaataccg agggtcagct
    10381 gattgaacac tgtttagt
//
LOCUS       DACEUZ010000097        10155 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.097, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000097 DACEUZ010000000
VERSION     DACEUZ010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10155
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.097"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            <1..1118
                     /locus_tag="IXG44_002015"
     CDS             <1..1118
                     /locus_tag="IXG44_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ZinT/AdcA family metal-binding protein"
                     /protein_id="HAQ1658723.1"
                     /translation="GHTHELDPHVWLAPSLAIKQVANIRDQLIEAYPEKQEVWTKNAA
                     AYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALEYGLNQVSIAGLSSSEEPSAA
                     RIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVSLEVLNPLEGLTNEQIENGEN
                     YLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGYFEDEDVQDRSLSDWAGTWQT
                     VDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQTDIASIKISEKEMAFTFKNG
                     TTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAFRYIQFSDHTIAPADSEHFHL
                     YAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(1187..1915)
                     /locus_tag="IXG44_002016"
     CDS             complement(1187..1915)
                     /locus_tag="IXG44_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1658724.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(1928..3209)
                     /locus_tag="IXG44_002017"
                     /pseudo
     CDS             complement(1928..3209)
                     /locus_tag="IXG44_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
     gene            complement(3350..3952)
                     /locus_tag="IXG44_002018"
     CDS             complement(3350..3952)
                     /locus_tag="IXG44_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory regulator AgrB"
                     /protein_id="HAQ1658725.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            4197..4403
                     /locus_tag="IXG44_002019"
     CDS             4197..4403
                     /locus_tag="IXG44_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1658726.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            4715..5434
                     /locus_tag="IXG44_002020"
     CDS             4715..5434
                     /locus_tag="IXG44_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAQ1658727.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(5515..6198)
                     /locus_tag="IXG44_002021"
     CDS             complement(5515..6198)
                     /locus_tag="IXG44_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HAQ1658728.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            6416..8602
                     /locus_tag="IXG44_002022"
     CDS             6416..8602
                     /locus_tag="IXG44_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAQ1658729.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNISVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
     gene            8948..9358
                     /locus_tag="IXG44_002023"
     CDS             8948..9358
                     /locus_tag="IXG44_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAQ1658730.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            9372..9728
                     /locus_tag="IXG44_002024"
     CDS             9372..9728
                     /locus_tag="IXG44_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HAQ1658731.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT         3664 a         1674 c         1924 g         2893 t
ORIGIN      
        1 aaggccatac acatgagcta gacccacatg tatggctagc tccaagttta gcaatcaagc
       61 aggtagcaaa catacgtgat cagctgattg aagcttaccc agaaaaacag gaagtatgga
      121 caaaaaatgc agcagcctat accgagaaac ttcaagcact ccatcagctt tatcaagaaa
      181 cctttaagca agcaaaacaa agatcttttg tcacgcaaca tgctgcattc aactatctag
      241 cgttagaata tggattgaat caagtatcga ttgctggtct ttcttctagt gaagaaccgt
      301 ctgcggcgag aattgctgaa ctgaaacact ttgttaaaga acatggaatc aactatattt
      361 attttgaaga aaacgccaaa gatagtattg ccagaacgtt agcaaacgaa gcaggtgtat
      421 ctttagaagt attgaatcct ttggaaggat tgacgaatga acaaatagaa aatggagaaa
      481 actatctttc tatcatggaa gcaaacctcg aagcgttgaa aaaaacgaca gagacagaaa
      541 acccgctaga agatactttg acccccaaaa aagaaaagag cgtctacaac ggttattttg
      601 aagacgagga tgtacaagat cgctctcttt cagattgggc aggaacttgg caaacagtag
      661 atacgtatgt agaagatggg acttttgatc ctgtttttga atataaagaa aaattgaatc
      721 aggataagac agcacaagaa tataaagaat attatacgaa gggctatcaa acagatattg
      781 cttcaatcaa gatcagtgag aaagagatgg catttacgtt taagaatgga acaacgaaac
      841 gttcaaatta tcgttatgta gggaaagaaa tcttgaccta cgaggcagga aaccgtggcg
      901 taaggtatct attcgaagca gaagataagg aaagtggcgc ttttagatat attcaattca
      961 gtgatcacac gattgcaccg gctgactccg aacattttca tttatatgca ggaaatgaaa
     1021 gtcaagaagt attgctgaaa gaaatggaga attggccaac attttatcca gatacaatgg
     1081 atggacgtga aattgcacag gaaatgcttt tgcactgata ggcaaaaaag aaccgaaggg
     1141 tttggatcgg ctggtcattt agtcggtcca aactattcgg ttctttttat gaaatcaatt
     1201 tctttaatcc acggatagag gctgggatgg tctcgccgtt tgccaacgtg acttctctgg
     1261 tttttttatt aatcgaccgc acattttgtg tattgataac ataggattta tgacaacgga
     1321 taaaattccg atgcattttc tcgatttctt tgatcttctc atagaattgt aattgggcat
     1381 gagtcaaatg gacttccagt ttatgtggcg ctcctagcga agtagaaaaa tattggatgt
     1441 ctttgatcgg taaaaatttg atctcatcat taaactctat tttgaagata tctttttgga
     1501 tttgggacat ttgaaaatct tcgaaaattt tgctcaggat atgctcgaca ttttctttta
     1561 aatcataaag atcttctttg acgatataat cagtcggttc gatgttgctt tttaagattt
     1621 tcatcgacat ttccgtatgg gaagtcacat agattatttt tcctaaagga tctaattcac
     1681 ggatcaattt accgatcgct acaccatcca tttcactgtc ttctagttcg atatctaaaa
     1741 agtaaatacc aaaagaagaa gttctgatct tggctgcatt gagaagttct tccggatcag
     1801 aggtagaaat atggacataa ctgtctaagt tttcaatcat gattctattt ttgatcaccg
     1861 ttgataaaac ttctaattgt tttggatcgt cttcacaaat ataaatggga atcatatcat
     1921 tactccttta ttttatttgt atataaatcg tttgagaaaa acgctggttt tcaattgatg
     1981 tttcaaggaa aagataagga ttggtctttg tcaattcatc caagataaac aagccaaggc
     2041 cttcgttttt tggatttgtt tttgttgaga accctttttg tttcaactgg tttaacggta
     2101 atgtatttgt tgttgtattg atcacacgaa tcatcacgga atgattcttc ttgagtatgg
     2161 tcaattcgat ttttttcttc tttgcagcac tagctccttc aatcgcattg tccaagataa
     2221 taccaagcat acgtacaagg tttaccgtat gtttcgtatc cagttcgatt ggctcaggaa
     2281 aatgaagctg gacgatcaac cctttttcct gtgccatcat gagttttaat gacagtaaac
     2341 tacggatctc aggaactttc atattatcca acatacttag attggccgga aaaaggttca
     2401 tcatctggcg tgtaggtaat atcgtttgat cataataaga ccgtaatcct tctaagtcgc
     2461 cttctgaaat atagctgtga agagaaaaaa gcagattttg gtaatcatgt ttgaattctc
     2521 tcaagtcttg atagtttttt tctagatctt tgatataatc ctgttgcatc ctgacttgtt
     2581 gctctaataa ctgatcagct aatttcttat tagagatttt ccaataataa accaagatga
     2641 tcaggccaac acacagatat agaatgaaaa aactcgtatt caaagctaag acatcaggtg
     2701 tttcaccaga aaaacgggta taaaaaataa tgatatagta tgtaaataat atcaatgtcc
     2761 cgatcgtacc gatcatacgg cggtccacag tgtgaaacca agtctctaac gattttttta
     2821 aacaaaaagc taataaagca gataaaaaag cggataagac aatgtgaaaa tactgcaatg
     2881 aatctcctgg ttcaataacc gaagatttca aaacaaaaaa atcgccgatg cttgttaagt
     2941 gatctcctaa aagcgacgtc aataacccaa aaagaaagcg cgtaaaccaa ttgttgacgc
     3001 gaagtcgaaa agaagaaaaa agtaagcatc agcattccta tagcaattag agcagtaaaa
     3061 atccctaaaa tcgaatagac ccatccaatc aaaatacaag aaatcagaat gactcccgca
     3121 aacagatatt tgtatcggat gtgatggtca atcaataaga tacaaattgt gtaaatcaaa
     3181 tattgcaacg ctatggacca taaaaacata aagtacagct cccattgatt tttaccaaat
     3241 tatagcacaa ttggcgtatt atttttttaa tttttcaggg attttgaatt cagaactcat
     3301 accaaagcag cagccagata caaaaaagtc agataagtta gttaattgtt taatgattgt
     3361 ttttttcatg aaagattcct ccaaaattca tcaagtagtt tgtaatagga agcaacgtag
     3421 ctatttctaa aacaatccca agtagcaaat aggtagaaaa gtaagaagct gagaaaaata
     3481 acgtaatgag agaaaaaatt agaaacatgc tgatcgcctt ctttaataat gggggacgtt
     3541 tctcgggttc aatgggattg attgctgtac ctcttgatcc atagcgaagc agtaacaaaa
     3601 agcagctgat taacaaaaag accatcattg ggaaggagat agtcaattgc agatgtttca
     3661 tcaccaaggg aacgccccat aaataaagta gtgtccacag cagacaaacg aattcagagt
     3721 cggaatgcaa tccataagca tatctgcgga tgcagatata agaaagaaag gtgattcctg
     3781 tttccgccca tgagcctgtc accagagaaa agactagaac gaccgctagt tttccaagat
     3841 tattcaataa aacttctaag acaaatttta cttggacata caccatatct tcttccgatt
     3901 tgtctccgct gaataaggct tctaataatt tgttcacaac tttatctttc atctcatacg
     3961 tacctccacc acaaatttta tgctaaaaaa aataaaaaac gtcttttgtt tcataaaaag
     4021 tggttttgac atcaaaaccg tcatgatctc ctaaaaaaat cttcgttttc caagaacctt
     4081 accaaaaaaa gtgacagttt tgatacgaaa agtacttttt atgacaagtt tgtgcttttt
     4141 cgagtgattt atgttaaaaa attactgacc ttataaaagg tgatggaggg attcttatgg
     4201 aaaatgaaca attgtttgaa gtcggcgaaa catatgcatt taaatctgat ttatttgaat
     4261 caccaataaa aggaaaaata gaaaaaatct atgaaaatag tgtgatgata cgtgtcatga
     4321 aatgcgcagt agaagacaat gataccgctc ataatctgca ctggcagtta gttgtacgca
     4381 aatcaagtac aatagaagaa taaaaacaga taaaatgttg agagcataaa gaggttgtga
     4441 gtctcccgtt tagctctgag ttacaaggaa taatgttaaa aaacagcttt tcatgtttta
     4501 tgaatattgc cttgtgaccg aggtgttggc aggagaatac tgtttatttg aaaaaagggc
     4561 tgcgacaaga cttttgtcac agcctttatt ttcatgaata ggagttactt ttggctactt
     4621 ttagagaagt agcttttaag gagcagataa agtcaaggct tttatttcta taaatgaagt
     4681 agtaaaatga agaacagatt ttattacagg aaggatgccg ataattgtga tcattgttgc
     4741 aggaacgatc ggtgcaggga aaagcacact aactgaaatg cttgcccaag atttagaaac
     4801 aaaacctttt tatgagaacg tggaagataa tgaagtattg ccgctatttt attctaatcc
     4861 agaaaagtat acttttttat tacagatttt ctttttgaac aaacgctttt tggcaatcaa
     4921 agatgctttc agccatgatg acaacgtact agaccgctcc atctacgaag atagtatgct
     4981 gtttcatttg aatgcagatt tagggcgtgt ttctgaagta gaagtaaagc agtatgaagg
     5041 tcttttagaa acgatgctga aggagttaga agaaatttcg ccacaaaaga aaccagatct
     5101 actggtttat attcgtgtat cctttgaaac tatgttagcg agaatcaaaa aaagaggccg
     5161 ggaatatgaa cagctagaac aagatcctga actctattcc tactataaag aactcaatcg
     5221 acgatacgaa gagtggtatg aacagtttga tatatgtccc aaaatagtga tcgatgggga
     5281 caaatatgat ttcgtagccg atccggcatg tggagaacaa atcgtacaag aaatcaagat
     5341 gcgggcaaag aaaatgacag aagaggcaga tgcccagtcg atccgctaca ctacaaataa
     5401 cccgaaagca aagggaatct ttacaactaa ataatataaa aactaagcaa attgctcact
     5461 cgcagatatt tctctgtgag cagcatcttg cttagttttt ttgattcatt gagatcaaat
     5521 agtgaaccga ttcgtatgat aaaattgcca taaatgaaat gtctgagtga gggccatgat
     5581 caaaaataaa atggaaacga tcaaaatcga atgggcgcca aacagatgat gacagaaaac
     5641 aaggacaata gctcctgcga taaaggcaag aacgacactt agatagtgga aaaatcgatc
     5701 aaaatctgct ttttctttag aaatacaata atcatataag ttcgttgcta ccccttttaa
     5761 atttccagtt gtaaataaat tagtgtaggc acgcccgttg attttatcaa aagctaccca
     5821 ctggatcgat gcagtaaaag caatcaaaat cgttactaat gcgggatgct catggacggg
     5881 tgtcaaacta acaaagaaaa aaatcactgc ttcataatat agaacaaaca agcgccagaa
     5941 atgtttttct ttttttctaa aataatcaat tagatacttt gttagaagaa tccctaagaa
     6001 aaagaaaagg gtagacaata cttttttacc aacacttagc cattctccgt caaatgcttg
     6061 gatgatcgat agaatgatat tgccagtttg agcagaggca aaagcaccat attggatata
     6121 agtataactg tccatggctc ctccgataaa tgtcagcaaa agtcctattg tacgcccctc
     6181 gtgaaaggaa gtctgcattt ttttcatcac cttttatgaa tatttttgtc ttccttatct
     6241 tagcataaac gattgaaaaa aaggaaaaat tttcagaaaa ctaaaatcat atcgcttccc
     6301 attttcattt ttagaaaggt ctgttagtat aaagaaaaaa tagacaacga aactttaaat
     6361 aaccaacttg aaataaacaa attcaaagaa gaaagatggg aaggaaatca atcagttgaa
     6421 tgaaaaagaa attctccgat tacaaaaaga attgaaacgc tgggagaaca agtataaaaa
     6481 gcttcaacaa gtctttcggc tacaagaaga aatcattgct acctatgaag cggcttatgg
     6541 cgaagcaccc ggacttattg aggaagaagt acaagtaaaa gaaagtgaag gaaacagctc
     6601 tttctttaaa atgatggaga aacgttccta taatcgcttc aatcaagaac aaaaagcagc
     6661 tatcacatat gatatgaaga aacatcttcg aatcatagca ggagctggga gcggcaaaac
     6721 gcaaacaatc tgtgcaaaag ctgtttatct gatgacgcaa aaacaagtag acgaagaacg
     6781 catactaatg atcactttta caaggaacgc tgcaaatgaa ctaaaaaaac gagtagataa
     6841 tttcagccag agaaaaaccg ctgtccatat cggaacattc catagtatct tttttaggat
     6901 atacaatgaa atttgtcgaa aatttcctga agtagcaatg caaggaatac aaggagactt
     6961 ttcaaaggat actgcacaaa aagtgaatgc tgttttgcaa cagctgatac gtaaatataa
     7021 tctttatata tttgatcaat atggagaaaa aacgattgct tctcggttag actattggca
     7081 gaatatgaac ctttcagtag aagaaatggt tcagttagtt aaagaaaaat atgattccat
     7141 tgataagaat gcacgacagc cgatatcaga acgactgtat ggattgctca cggaactcca
     7201 agaacaaaaa agaagccagc aattgctaga gttcaatgat gtactccaaa atctgaaaag
     7261 cgctttggag aatgaggaga tccagcatta tatcggccaa aaatatgatt atctattcat
     7321 tgatgaattt caagatacta atccacttca atgggaaatc gtgaaacgga tgacaaagga
     7381 aaacaggatc aagttgattg ttgttggaga tgatgatcag agtatctatg gattcagagg
     7441 atcagagcca gcatacatca agaactttga aaaagaatat cctaccaaaa ctcttttctt
     7501 attaactaat tatcgttctc gggcagctat tgtccaagga gcaaatagat tgatcagcta
     7561 taataaaaat gatcggatac caaaggcgat gattccagca caaaaagaag caggtgttat
     7621 ggaagcctat ttgttttctg atactaaagc agaaagtcag tggctaatca aacaaattca
     7681 gacatttatt caaaaaaatg ggaaatataa ggaatcaatc atattatacc gatcagcttc
     7741 acaaacgcag cagcttgtcc aatctctgct gaaaacgaat attccttttg tactggaagc
     7801 agatagtcca tatgaaggta tctttggtat caagtctttc caacatttct atcaaaaaat
     7861 tgttcattgg caaacagcac tgaatctgca aaagaaaaat caggcctacc agcaattgct
     7921 ccggcagctg atggcagact gctacctgaa aaaaagtgaa tgcgatcaat acttttctcc
     7981 taaatatcaa aatatatccg ttgcagatta tattttgtcg ataagcccca atttaaaatc
     8041 gaaatctgca gaaatccgag agtttgagga agcgttgcgt aaaacagacc agcatcaaga
     8101 aatctattcg cttgttcaaa gctatcttca tctgccacgt atagctaagg aaatcgacca
     8161 gagtgatcaa gaatggatac gagaagaagt aaagcagcat gctactttca gaagactcca
     8221 agccttatcg caagaaaccc aagcgacagc taaagaatta aaaacccgct tgattgctta
     8281 cagacaagga aaacttgatg cactatgtat ccagagcatt cacaaaagta aaggtcttag
     8341 ttaccgaaat gtctttttga ttggttgtaa cgaaggaagt ttgccttata acggagcgac
     8401 agaaaagaaa gtaatcgact ttaatgaaat aaaagcagaa ccagtaacga cgatagaaga
     8461 agaacgaaga ctattttatg ttgcgatgac tagagcaaga aatcgtctgt atctatgtgt
     8521 acctcaaatg aaaaattcaa gaaaattgaa agtatcgaga tttatcaaag aaaccgggag
     8581 tcgtgtgaaa aaaggaaagt gatagaagag agggtgtgac agaagtggtc agtttcaaga
     8641 aagaagaagg aatgtacgaa aattgcttct caaatttttg tgaaatttca gcttattttc
     8701 gaagaagcta tttctggaac acagtttatt cggaaaaggg aggtgtgaca agaattttgt
     8761 cacacctccc ttatttattt tgaaaatgag aatactatta tcaaaataga gaatatatag
     8821 aaaaattctc attattcaaa cgtaaataat cagttatttt gatttttttt atattttatt
     8881 agctaataat tggtatttta aaagaaatta ctgtagaatt ggattataat taaaaaggag
     8941 tgagaaaatg ataaaagtat atacttcgcc tagctgtact tcctgtagaa aagcaagagc
     9001 atggttgcaa gcgaatcaac ttgaatttga agagaagaat atttttgccg aaccgttaac
     9061 agaaagtgag atcaaaaaga tcttggcagc gactgaagga ggagttcaag atattatttc
     9121 cactcggtcg aaaatttacg aaaagctggg catagatttt aacgaattaa ctttaaagca
     9181 aatgatcgtc ttattcaaag aatatccttc attattgaga cgacctattt tgattgatga
     9241 aaaaagaata ctaatcggat ttaatgaaga tgagatccgg tcatttgtcc ctcgtgaaat
     9301 tcgatgtgtt gccagaaatc tgatctattt acaaaacaat aacatcttaa attcataggg
     9361 agggagcctt cgtggaagtg aaagtctatg gatcagcgca ctgtgtaaca agtctgaagg
     9421 cgaaacaatg gttagaacac catcaattgc cttatcaatt tgttgactta gaagaacgcg
     9481 atctaagtac agaagaagca gaagaattat gcagttttga agaagtgcaa gcagaacagc
     9541 tatttgcgac ttggtctgag gcttttcaca acattgcgat tgacctttcc tgtgtaggaa
     9601 aaaaacaact gattttttta tgcacacaac ataccaatct cctcagacgt cctttgatta
     9661 ttatcgatga agttctattt attggataca atgagcagtt gatggaacag cttatcttaa
     9721 ataaatagac tttaactgtt ttaatcaagt cactgagcat ttcatacagt tgtaacagtc
     9781 gaacacttag catcaagcgt gttatgtgtt ctttttttga ctttattttt ctactataga
     9841 gacatgctca atttatgtgg tcaaaattca aatctaggtg gggatataga gttaaaaata
     9901 tattgacatg tgtaaattta catgatatat tcatttctgt tgttttaagt agcaaagcaa
     9961 taaaatagta ttttaaaaaa catataaaat agttgttgac atgttagaaa tgacatgata
    10021 agataactga gttgttaaaa aagaaccact tgtgaagctt gattctatcg aaaaaaactt
    10081 caaaaaagtt cttgacaaat aacaaatgat aagttatgat aaatgagttg ctgtaagatt
    10141 tcgaaaaacg aaatc
//
LOCUS       DACEUZ010000098         9873 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.098, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000098 DACEUZ010000000
VERSION     DACEUZ010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9873
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.098"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(21..266)
                     /locus_tag="IXG44_002025"
     CDS             complement(21..266)
                     /locus_tag="IXG44_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1658732.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(381..959)
                     /locus_tag="IXG44_002026"
     CDS             complement(381..959)
                     /locus_tag="IXG44_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ1658733.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSIISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(1036..1566)
                     /locus_tag="IXG44_002027"
     CDS             complement(1036..1566)
                     /locus_tag="IXG44_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HAQ1658734.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(1570..2430)
                     /gene="rlmB"
                     /locus_tag="IXG44_002028"
     CDS             complement(1570..2430)
                     /gene="rlmB"
                     /locus_tag="IXG44_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HAQ1658735.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(2423..2818)
                     /locus_tag="IXG44_002029"
     CDS             complement(2423..2818)
                     /locus_tag="IXG44_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAQ1658736.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(2815..4227)
                     /locus_tag="IXG44_002030"
     CDS             complement(2815..4227)
                     /locus_tag="IXG44_002030"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HAQ1658737.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(4224..4760)
                     /gene="cysE"
                     /locus_tag="IXG44_002031"
     CDS             complement(4224..4760)
                     /gene="cysE"
                     /locus_tag="IXG44_002031"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HAQ1658738.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     gene            complement(5082..6542)
                     /locus_tag="IXG44_002032"
     CDS             complement(5082..6542)
                     /locus_tag="IXG44_002032"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HAQ1658739.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(6712..7869)
                     /locus_tag="IXG44_002033"
     CDS             complement(6712..7869)
                     /locus_tag="IXG44_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HAQ1658740.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(7903..9273)
                     /gene="radA"
                     /locus_tag="IXG44_002034"
     CDS             complement(7903..9273)
                     /gene="radA"
                     /locus_tag="IXG44_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HAQ1658741.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKIAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(9336..9863)
                     /locus_tag="IXG44_002035"
     CDS             complement(9336..9863)
                     /locus_tag="IXG44_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HAQ1658742.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
BASE COUNT         2508 a         2214 c         1784 g         3367 t
ORIGIN      
        1 atctcaggct cgtttgcttt ttaaactgct tcagttaaaa attcgatctc aactgtccga
       61 gtaagtacat ctgaatagct gtaagatact cgttcaaaag aattttcttc aggatccaaa
      121 tcaacaacga atactgaagg ataggtttct gttaaaacgc cgcggcgttc tgtttgacgc
      181 tttcttcctg tctgagcaac caacatgatt gggctgccga tacgaccttc taaatctttc
      241 ttgattgaag ctaaagttgt tggcatacag ttcacctcta ctatcaacta taccacatat
      301 ttttaaaatt ttcaagttta ccataaaaaa aaaagaaatg caataaaaat aaataagaag
      361 ctcattaaga tttttctaaa ttattcctca ttataaaatt tcaagtgttt ttttattttg
      421 agttttactc tcccgtaagc gctcattact tgcttgtgat ccattgctaa acattcagcc
      481 atgtctacaa aatcaacgcc ttgcatataa taatgaaaaa ttttagcttc tatttttgaa
      541 aactggattt cgcaattttc gattgcttct tttaataaaa gatgctgaat cgctaaaggt
      601 tcttgcaatg tgatctctgc taaaaagtct ggtccttctg tctctatttt ttggtcgatt
      661 gagacttcat ctttttgatc acgacgcttc agcgcgttct gcttcctcaa cagagaatag
      721 atttgattgc ttataatgct tttataataa cttccaaagc taacaccttt gcttttttcg
      781 taagttctca atgcctgata taaagcgatc cttgcttctt gttcccaatc ctcagaaaca
      841 taatttctta aataataggt gtttttcaaa cgtagtaaaa gtggaaaata tttttgatac
      901 agggcagcaa atgcttcttc gtcaccatta cgtgcagcgc gcaataactc ctcagacata
      961 aaataattcc tccataaatt cgtttatgga ggaatcttat catcaatgat tcatcgaact
     1021 gattagaacg atatgttatt tattttttct cattaaatcg tccaattttt gggacaattt
     1081 ttttaactgc tcttcattcc atggagaatt acgtcggaaa tcaataaaat gcatatcttt
     1141 attttcttgg gctattgttt tttccgtttt ttttatcgtc ttatataatt cataagcaga
     1201 agttcgtaat gctccttggg aaaagatcat ccactgttct gctaaatcgc ttgtcgccac
     1261 tgtcacttgt gtcaaacggt cattcagctg accagcgatt ctctcgatat aactgtcagc
     1321 cgtttcgtct tctttcgtaa aaacaacatc caactggtat ttcttataat tttgttggat
     1381 gcccggaact agttgggcat cgaagacaac gatgacttcc attccttcat atttcgcata
     1441 atttgataaa cgatgtaaaa gagcttctct tgcatccgcc attttgtcct gctttttaag
     1501 ctggacaagt tccggccacg aaccaatcat gttgtagccg tctacaatca acagctgttt
     1561 tttcatggtc tactccccct gacgttggga aaaagcctga tacataagca aaccagcagc
     1621 aacactggca tttaagctct ggacgtgccc ggtcatcggg atcgtcagca attcatccat
     1681 ttcttttttc aatcctggac tcattccttt gccttcatta ccaatgacta aagcaattga
     1741 tcctttggca ttccattttt ggaagtttgt gccggtcata tctgtgccaa atacccaaaa
     1801 tcctgtttct ttgagctgtt gcacagcttg taccaaattc gtcacgcgtg caacagggac
     1861 atgttcgact gccccagtgg aagtctttgt tacgataggt gtgattccaa ctgctcggtg
     1921 ttttggtatg atcacaccat cgacgccact tgcgtctgct gtccgtaaaa tcgacccaaa
     1981 attgtgtgga tcttcgatgc tatccaaaat caaaaagaag gggtcttctt ttttctcttt
     2041 tgttcgttca atcagttggt caagggataa atattcataa ggtgtaatcg caagaacgac
     2101 tccttgatgc acgccgtgat cgctcaaagt ttctaatttt tgtttaggaa cccatttgac
     2161 tggcacagct tgttcacgtg cgatttcttt cagttcttct actttttctc ctcgtgaatc
     2221 ttcttgaaga aagagtttat tccctcgccc ttgttgtagc gcttctactg cggcatggtg
     2281 cccaaagacg aaattatctt ctaatgcttc ttcattttga tcagaaactg actgatttcg
     2341 tccagtttgc tttttatttg aatatctatt ttgttttttg tcttcaaatc cacgtttcga
     2401 ttgatgtttg tttgttcttt ctttacccat ttttttcacc aactttctga atacaccagc
     2461 ggatcaattc ttccatccgc tcggtttgtt ttgtcaaatg cagatagccc atgagcgctt
     2521 cgaaccctgt tgcgacccga tacgtcgtaa catcagcatt ttttgcgctc gtatggcttt
     2581 tagcatttct tcctctgcga taataaagtt cttcttcttc tgtcaaaaga tgttcttcca
     2641 gcatttgctg aatcaaaaag gcttgtgcat tcgcagaaac ataattcgtt gccgcacgat
     2701 gcagcttatt gggttttgtt tctccttgat caatcaggtg gtcacgaaca tagatttcgt
     2761 aaatcgcatc tccaacataa gccaaggcta aaccgttcaa ttgtgtgtaa tctctcatgc
     2821 ttctcttctc catcgggttc cttgcggtgt atcttctaat aagatccctt tttctttcaa
     2881 caaatcacga atttcgtcgc ttcggacaaa atcacgattg cttcttgctt gatttcgttc
     2941 ttcgatcaat tgttcaatct cttcatctaa taactcttct gctgtaaata aaataccgaa
     3001 gatcgaaagc cattgcgaga ataaagcttc tccttgtgcc aagacagcgg cagaaacttt
     3061 cggctggtct agataagtgt tcagccactt agcaagctca tagaccactg tgatcccatt
     3121 tgctgcatta aaatcatcgt ccatttcttc gatgaatcgc tgttctaaat tttctaacaa
     3181 agcgatttta tccgcatcat ctgccaaatt ctctgaggca gtttcttggc ggaatttcaa
     3241 attgtcaaac gtattcttca gtcgttgtaa attggtggct gcttctttca tcgtcgtttc
     3301 actgtagcgg atcggacgac ggtattgtgt ggtcgccatg aagaaacgca agacttgtgg
     3361 atcaacgttt ttgatcattt catgtacagt gatgaaattc cctagagatt tactcatttt
     3421 ttcatcgtct tcgccaatcg ttacatagcc attgtgcatc cagtaattag cgaatttttt
     3481 ccctgttttc gcttcacttt gtgcgatttc attttcatga tgaggaaatt ctaaatcttg
     3541 cccgccacca tggatatcga tcgtttctcc taaatgtttt gttgccataa cggaacattc
     3601 aatatgccaa ccgggacgtc ctttgcccca tggtgaatcc caagaaatct cgccaggttt
     3661 tgcttgtttc cataaagcga aatctaatgg gtcttctttt tgctgctgtt cttcccctgt
     3721 acgctgactt gctccgactt ccagttcatc gatggattgg tcacttaatt tcccataatt
     3781 ttcaaattta cgggtacggt aatacacatc acctgaagat tcataagcat agcctttttc
     3841 aaccaacact tgaataaatg ctaaaatatc tggcatatga tccatgactc ttggatgcag
     3901 tgtagcaggc tcgacattca acgctttcgt atcttcttca aatgctcgga tgaaacgatc
     3961 agcaacttct ggtgctgtaa tacctaattc gtttgctgta cggatgattt tatcgtctac
     4021 atccgtgaaa ttcgatacga aatcgacttt atagcctcga tattcaaaat agcgtcgaat
     4081 tgtatcgaaa gcaatggtgc tacgagcatt tccgatatgg atataattat acacagtcgg
     4141 tccgcagaca tacataccga catgtccctt ttgtataggg atgaattctt ctttttttct
     4201 ggtcaatgta ttgtagattt ttatcattct gatctcctca ctttttctcc tttgattcga
     4261 acgacttttg ctggtactcc taccgctgta gcgtcgtctg gaacatcttt caacacaacg
     4321 gcacctgcac cgattttggc gttttttccg atcgttaccg gtcctaatat ctgcgcattc
     4381 gcagagagca tggctccttg tttaacagtt gggtgtcttt tacccgtttc ttttcctgtg
     4441 ccgcctaatg ttacgccatg gaataaaatc acatcgtcct cgatttctgc ggtttcgcca
     4501 atgacgattc ccattccatg gtcgatgaac acgccagttc caatcgttgc tcctggatgg
     4561 atctcaacac cagttaggaa acgccagaat tgagcattga tcttcgccaa taaaaagagc
     4621 cggtgctgat ataaaaaatg agagaaacga tgccaaaaca aggcatgaaa accagggtat
     4681 gtcaaaacga tctcagctgt cgaacgtgca gccggatcgt ttctctttgc tgcatcaatc
     4741 gctcgggtaa accaactcat gactttccac tcctttcgta agaattttat gtaggaaatc
     4801 atcttcttat aaagaaaaga agcgcctttt aaataagttt ccttatcaaa agacgctttc
     4861 cgcgtggttc cacttttctt cataatcaat gattatctct gacagctaac gcctgttata
     4921 cgttcttggc tactttgttt cctataaaac agtttttcct gttttctgtg tttcgccaag
     4981 accactccca gaggcatttc ggcaaccatt cattgatacc ttgcaccacc cggtatctct
     5041 cttgtaatga tcagctgctt acttttttct gttcatcgtg tttagttcag tacttgattt
     5101 aaatgtgcta atgatttttc acgaccaagc aattcgatcg tttcaggcaa ttctggacca
     5161 tgcatttgtc ctgatacagc tacacggatt ggcatgaaaa gattttttcc ttttacgcca
     5221 gtttcttttt gaacagcttt gatggcagct ttgacagttg gagcatctac tacttccatt
     5281 tgttctagct gtgctttgaa tgcgtggaga acagtaggaa ctgtttcacc agcaagtact
     5341 tcttttgctg cttcattcaa tacaggatgt tcattgaaaa atagcgtaga cagatcaacg
     5401 atttctgcag catagctcat ttgaggttga tacaggctaa cgatcttctt cacccattcg
     5461 attgtctttt catctgggtt ttctgctaca cgtccatcag cgattagata aggcagacac
     5521 atgtcagtaa atgcatctag atccattgct ttgatgtaat ggttgtttac ccattgcagt
     5581 tttttcgcat cgaaagctgc gggtgattta cttaggcgtt ttggatcgaa cattttgatc
     5641 aattcttggt gttcgaagat ttcgtcttcg cccacaggtg accagcccaa taatgcaata
     5701 aagttgaaca ttgcatctgg tagatagcct aattcgcgat attgttcgat aaattgcaag
     5761 attgattcgt cacgtttgct tagtttttta cctgtttcac tgttgatgat caatgtcata
     5821 tgtccgaagg ttggtggtgt ccagccaaat gcttcataga tcatcagttg ttttggtgta
     5881 ttggcaatat ggtcatctcc acgtaaaacg tgagtgattt tcatcaaatg atcatcgacc
     5941 gcaacagcaa aattataagt tggcatacca tcgcgttttt gtatgacaaa gtcgccaccg
     6001 atattatcgg attcgaacga aatagcgcct ttcaccatat ctgtaaatgc gtattctgta
     6061 ttgcgaggca cacggaaacg tacaacagat tctagacctt gtgcttcttt ttctgcttgt
     6121 tcttcaggtg tcaagttcgc acatgttcct gcgtaatgag gcatttctcc tcttgcgcgt
     6181 tgtgcttccc gttctgcttc taattcttct tctgtgcaat agcatttgta tgctcgattg
     6241 ctagcaagca gctgatcaat cagcggttga tagatttctt tacgttcaga ttgacggtaa
     6301 gggccgtatt ctccaggatt ttcaggagat tcatcccaat tcattcccaa ccaagcaagg
     6361 ttttccaact ggcttttttc gccatcttcg atattacgtt tttggtcagt atcctcaata
     6421 cgaataataa agtctccatc attgtggcgg gcaaacaaat aattgaataa tgcagttctt
     6481 gcatttccaa tatgcaggtg cccagttgga cttggtgcat agcgtacgcg tactttcgtc
     6541 attttcttcc tcttctttca aactagactt tgactaacag caaaaattcc gtcagttacg
     6601 gcttctcaaa tatcatttat ttaaagccac tcagcacaaa ttttaccttt tctaaggtaa
     6661 atagtaaagc caactggttg aatttcctta ccagttggct gaatttcttt tttattttga
     6721 ttgtttagtc tttttctctg tctctttttt attgtcgatt cctcgtcctg catgtgcagg
     6781 tttagcaaag atcatgcgtc cagcagctgt ttgaagggca cttgttacaa cgacttcgat
     6841 acgttcattc atgtaatgct gtccatcttc tacgacaacc atcgtgccgt catcaaggta
     6901 ggctactcct tgttggcgtt ctgtcccggc ttttactact agaacattca tcgtttcacc
     6961 agggatgacg actggtttta cggcatttgc caacgcattg atattaagaa caggtacatt
     7021 ttggaattca gagactttat tcaagttata gtcattggtt acaatgacac catctaaaag
     7081 cttagccaat ttgatcaatt tgctgtctac ttcactgata tcttcaaagt ctccatcgta
     7141 catttcgaca gatataccct cttctttttg caaggcattt aggatatcta atccgcgacg
     7201 accacgcaca cgtttcaaac tatcccctga gtccgcgatg tattgtagtt cgtaaagaac
     7261 aaaattaggg atcatcaagg ttccttctaa gaatccagtc tttgtaatat cataaatccg
     7321 accgtcaatg atcacgcttg tatccaagat tttatattta tggaaatttt ctcctgattt
     7381 tcgttctaac agttgactct caacttgact agctgtttct tgctcgattt ttttgcttcg
     7441 tgaaccaaaa actttcttcc attcttcgat tcgtgtcgtt cccattctca atcccagata
     7501 gcctaagatg atcatcagta aaatcggcaa tacactattg acgaatggaa tttccatatt
     7561 gtaaagaggg gtcgagatca caacacctaa agtcaatccg atgatcgcac cgatcgcacc
     7621 gaagagaaga taagtcaagc tgacctcact tagtttttgt tctacttgtt tgataatagc
     7681 tgagatatgt ttggccaaca gcaaagataa taaaaagaaa ataagtgcgc ctaacaggct
     7741 gttggtaaat gtattattca gccatccgtt tgactgttga ccgattgcct gccaagctga
     7801 gggtaaaaat gaaataccca agcttgcgcc gacaatgatc atcagcaaag taatgacacg
     7861 tttttgcatc gtttttcctc cgtttcgaca atcatttatt agttagcgga acacacgctt
     7921 caacgtttca gccaaagttg ctactccaac tatctcgatt ccttttggcg cttcccagtt
     7981 acctaagttg tttttaggta ggtatacttt tgtgaagccg agtttttgga cttctttgac
     8041 tcgttgttca atatgactga cacgtctgat ttctccagtc agtccaattt caccgatgaa
     8101 acattccgtt ggctgtgttc ctttttcttt atagcttgaa gcaatactga cagcgatagc
     8161 taaatcaatc gctggttcat ttaattttac cccacctgca gcttttaagt aagcatcttg
     8221 attctgaaga agcaacccag ctcttttttc cagcactgcc atgatcagag atacccgatt
     8281 gaaatcaaga cctgttgttg ttcgtttggc atttccgaac atagtaggtg tgactaatgc
     8341 ttggatctct accaaaattg gacgtgtccc ttccattgcc acaacgatag cagagccagt
     8401 tgcaccgtct aggcgttctt ctaagaaaat ctgtgaagga ttagcgactt cttccaatcc
     8461 atgctcatgc atttcaaaaa tcccaatctc attcgttgag ccaaatcggt tttttactgc
     8521 tcgcaaaatc cgaaaactat gatgttgttc accttcaaaa tataacaccg tatccaccat
     8581 atgttctaac attcttgggc cggcgatcga gccttctttg gtaacatgac cgacgataaa
     8641 aatggctatc ccatttgttt tagcgatttt gagtagttct gcagtggttt ctcttacttg
     8701 gctgacactt cctgccacac ttgtgatgtc tggctgcgtc atcgtttgga tggaatcaat
     8761 gataacataa tcaggggaga tatgttcgat ggcacgactg atttcgttca tatctgtttc
     8821 agcgtataga taaaattctg tgttgatact tccgaggcgt tccgcgcgta attttatctg
     8881 ctctgcgctt tcttcgccgg atacatataa gacttttccg cctatggctg ctaattgctg
     8941 agaaacttgt aacagcaaag tggatttccc gatacctgga tcgccaccaa tcaagaccat
     9001 agaccctggc accacacctc cgcctaacac acggttcagt tcttctaatt ttgttttgat
     9061 ccgaggttct tttttaggga cgacgtctgc gatttttgtt ggttttgctt tttctcctgt
     9121 caaacttgtc cgagttctgc ggtccgttgt atcttgttcg atttcttcga ccatcgtatt
     9181 ccattttccg caatttgggc agcgtcccaa gaacttaggc gacacatacc cgcaattttg
     9241 gcaaacaaat tgtacttttg atttttttgc cattgtaccg cctcctttta tcctaatcat
     9301 gagacattaa atcaagctta agtatcttta acgttttatt tactggaaga accaaatccg
     9361 ccttgacgtt gagcagtcgt ttgatcttga tctgctttta aaaacggcaa aaagatccct
     9421 tggccgatac gctcgccttt tttgatcact acatccttga aaccaaagtt ggtgaattgg
     9481 aacatgatat ggccttcatt tgcctcatta ttgtaataat cactgtcaat cacacctact
     9541 ccgtttgtca acactaaaaa ccgcttcaac ggattgcttg aacggttagc aagctgcaaa
     9601 aattcatctt ctcccatgta agctttgatt ccagtaggca cgagggttgg tttcaattgt
     9661 ttttcatttt ctgatacatc ttttttcaat ccatctgcga ctgtttccaa cccctttttc
     9721 cagcctgctt tccagatact tggaatcacg acctcttctg ctgcttcaaa atcatagcca
     9781 gcagcatgat gtgtagcgcg ctgcggtaga ttgatatttt gatctttata tttagaaata
     9841 acttcaaatc ctctttttct caccggtttt cct
//
LOCUS       DACEUZ010000099         9067 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.099, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000099 DACEUZ010000000
VERSION     DACEUZ010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9067
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.099"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(558..1598)
                     /locus_tag="IXG44_002036"
     CDS             complement(558..1598)
                     /locus_tag="IXG44_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HAQ1658743.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLL
                     KDRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGR
                     VNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDTTVIAKIANTAKPREINDTT
                     VIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVD
                     IMKEDSINTFVPENVLNLIKTFSSTYEDAQKTVQTIHNAKKKAEELEQTVVVYEELES
                     YGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETFN
                     ELPTVSTENWLE"
     gene            complement(1947..2273)
                     /locus_tag="IXG44_002037"
     CDS             complement(1947..2273)
                     /locus_tag="IXG44_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1658744.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNFKSKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQNKI"
     gene            complement(2260..3048)
                     /locus_tag="IXG44_002038"
     CDS             complement(2260..3048)
                     /locus_tag="IXG44_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ1658745.1"
                     /translation="MARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVDIDPQG
                     NATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAEREFTQVG
                     SEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLKGLIKL
                     SETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTSAATEISRVIGAKIFKTYIRRT
                     ITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
     gene            complement(3842..4021)
                     /locus_tag="IXG44_002039"
     CDS             complement(3842..4021)
                     /locus_tag="IXG44_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1658746.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHNFRFYIFPLYI"
     gene            complement(4018..4368)
                     /locus_tag="IXG44_002040"
     CDS             complement(4018..4368)
                     /locus_tag="IXG44_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1658747.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIIGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            complement(4361..5686)
                     /locus_tag="IXG44_002041"
     CDS             complement(4361..5686)
                     /locus_tag="IXG44_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAQ1658748.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSLNLFTTEGTRSQRRKKLAQMIQENIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFVAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGIMSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLHIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGNGGIKND"
     gene            6136..6750
                     /locus_tag="IXG44_002042"
     CDS             6136..6750
                     /locus_tag="IXG44_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1658749.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(6915..7739)
                     /locus_tag="IXG44_002043"
     CDS             complement(6915..7739)
                     /locus_tag="IXG44_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1658750.1"
                     /translation="MIERLKDFMISVWDVAMLADESNYTSSIYRLEDSNWIEISLELK
                     TLPNSVNYIIRDITSEAQQLVNYMHGHESIPFSLILLKRSKNISNTRDRFISIITACE
                     IGVKEFYQQEKPDLTLIFENLQSPPVDKLLGSMFKGYFNIEFPKEIRKEIKNFIEKRN
                     KLVHSPQGSKPTIEECFECYMAVLKTFNFFYKINDSFMYRDFYEESIELTPTEPNKSQ
                     ITLSKELERKVESQELAIGISITNVSTYKELYEAKSQDNKDFVENIDKEPTNTVGD"
     gene            complement(8377..8634)
                     /locus_tag="IXG44_002044"
     CDS             complement(8377..8634)
                     /locus_tag="IXG44_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAQ1658751.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
     gene            complement(8627..8896)
                     /locus_tag="IXG44_002045"
     CDS             complement(8627..8896)
                     /locus_tag="IXG44_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAQ1658752.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
BASE COUNT         2811 a         1616 c         1277 g         3363 t
ORIGIN      
        1 gttgcgggta ataccgtaac actgaagggc acgcattaag gcgtcatctt aatgctttcc
       61 tgtaggacac tgacggagat taccagtctc ccgagcctta caggggcctt tctttttttt
      121 gtattttcaa ttatcacaat ttatcgtcca aaagtgcaga acgaaaatca attcatcaca
      181 agcatatcag acaattaaat ttaaaacaac taaaaatata caaaaaagtg taattgaata
      241 ttagcagcac tgcctgaaaa gaagtaatat caggcttttc agcttgggga aataagtgaa
      301 ttacctctct tcatgaatta ttataactca aaaaagcaca tatgcaaaca ctagttccct
      361 atttttttga atgaaaaata actaaaatat ctaacgatta tgactttaaa acctacgttt
      421 gtgattgttt tcataaactt ttaatctaaa aactccggaa taatagctat ttatttgtaa
      481 catttgtaat aaaattaaaa tcataaaaaa gttctaattt aaaattgatc atttttatag
      541 tgatcgcttt ttatcaacta ctctagccaa ttttccgtac taactgttgg taattcgtta
      601 aatgtttctc tttgttcgtt tgcaagcttt gccgcaatag ctttttcgac aaaccaattt
      661 tttacataaa cgaaaattag attctgcaaa ttgtttactt tttcagtttt ttgtttttga
      721 taagctttca gcaaagtcat atataatcct ttgtcagcat caattccata agattccagt
      781 tcttcataaa caacaactgt ttgctctaat tcttcagctt ttttctttgc attatgaata
      841 gtttggacgg ttttttgagc gtcttcgtaa gtactagaaa atgtttttat caagtttaat
      901 acattttctg gtacaaatgt atttatactg tcctctttca tgatgtcgac gaagttatct
      961 agtaaaactt gattttgtaa ctgatcagtt tcagtgtcta ttatgtgtct attagtgtct
     1021 atagaattag aatagaatag attgtctgcg attttcgcaa ttacggttga gtccttgata
     1081 tgactggatt ttgccgtatt tgcgattttc gcaattacgg tggtatcatt gatttctcgc
     1141 ggttttgccg tatttgcgat tttcgcaatt acggtggtat cagtattgtc ttcctcttcc
     1201 atgattttaa agacgtcttg attcgttact tctggcttgc ctaagtacaa taaattaggc
     1261 atggattcaa tcttaccgtc acgtttattt actcttcctc tcttctgttt cagaagacct
     1321 gctttttcta attccttttt gattttagat actttacctt cgcggcattg taacaatttc
     1381 attaattctt caactgtgaa tatgaaaaat atattatcat cagaatctac ccagttattt
     1441 ttgactgaat aatcaaatcg atctttaagt agcatataag caatttttgc atcatttgat
     1501 agatccatgt atttttcgtt agtaaaaaat accttaggca tttgataata tttttcacga
     1561 taaatttctt ttgatttgta ataattaaaa tcattcatag ccaatcctct ttctgtagga
     1621 tagcgttttg ttcaaaacta tgttacaatg aattaaacaa atatatctat ccactatata
     1681 tttttctact gctgataagt cgccaaactt tgttaaagca gtagtctttt tttatctttt
     1741 tttagtttac cttaaaaaat aaattaaagc aaaccgctct atttcaaggt ttaagctcat
     1801 agtaactata tcacgatatt acaataatat tatatcacga tatcgtaata ttacaatatg
     1861 aagataagcg acttatcagc tttgatacta ctgattttta taaaaaaaat atcacgatat
     1921 tatgatatcg tgatattgaa tgtaaattaa attttatttt gtttctttgc tagtttatat
     1981 ccttcaatca agtattcttc aggtatggac tttaatagcg catcttctat aacgtctggt
     2041 ttacttttgt ctgattcagc aatagtcgtc caatatttga gtgctgacat tagaattgga
     2101 tttatacgaa tgctatctgc tgttcttaca ctacttcctt gatcttttat gactttactt
     2161 atatttttct tggttaattc gatttcttct acttcttttg atttaaaatt ctttttattt
     2221 ttatttccta gtaatctact tactgcagct tgactgcgat tatcttctgc catcaaaatc
     2281 accaacttct tttaaatatt cttctgtaaa tgctttatag tcatcttcag ctgttgaggg
     2341 tttattaaaa ttgattagat ctgatccagc agcttgcgct tcgtcaacgg ttattgtcct
     2401 acgaatataa gttttgaaaa tttttgcacc aatgactcta gaaatttcgg ttgctgcaga
     2461 cgtcatttct ttagaaatgt tataacgctt attatactta gtaaatagaa ctcctcgtac
     2521 ttcaagcttg tcgttcgtat attcttttac cgtttcaata gtttcactta actttattag
     2581 tccttttaat gagaaatacg cagcttcaac tggaatgata atttcatcag aaacagtaaa
     2641 tgcgttgact gtaagcattc ctaaacttgg tggacaatca attataatat aatcatagtt
     2701 gtccttgatt ggttgaagta ctcttttaag cctgtgttca cttcctactt gagtaaattc
     2761 cctctctgca gaagaaaggg ctatgtcggc tggcaaaaca tcaattcctt tatagtccag
     2821 aatagcatct gtaatgtcag cctccccttt taatacttca taaattgtag gttggtttgt
     2881 tccatctata tcgaaaccta cattatctgt agcatttccc tgaggatcaa tgtccactaa
     2941 aagtacattt ttaccgtaaa caaatttcaa acatgctgct aaattaacgc tagtcgtagt
     3001 tttagaaact ccaccttttt gattggctac tgacaatatt cttgccatta aaatatcccc
     3061 cattcatctt atattataat ataattatat cataatattg tgatattgca atatcgtata
     3121 tgcattatat tgtaatattg tgtgattatt ttgttttaaa gatatatatc ggttcggata
     3181 taaagattat tcatatgtta ttcgaaatta aaataatcac tttttattac tttaaaattt
     3241 tttattaatg ataagaaata tgttttgcga gcagcaacta gaataattta aacattacta
     3301 aactgtctat attgttacta ataattccct tcttatagtg cattttctta gaaaaaaaca
     3361 atattacgat atcatgatat taagatatcg taatattata caattgtgat tgatattata
     3421 tatgtttttt tattttttta tcatgtttaa tcttgaagga atcatattct ttttctaaag
     3481 acaggagctt ttcataaaca tcagacatgt ttaaactaga ggccaggctt aaatcataaa
     3541 cgaatgaagc aggtaatgat tttattctac gttgtcgggt tatccagctt gcaaccgttc
     3601 cttgtgggta ggcatataac tcacaaaatt gttctacagt gattcctaaa ttcttgataa
     3661 tgtaaacatt gatcgggtgc gagtaaacaa atgtattgat agacatggtg cgtaagccta
     3721 ctttctttta ttattgcgtt tgcaataata gtttaacaca cgcattattc tagtatctac
     3781 tcctatttgt aaaaacaaaa aaggattcat agagataact ctatagaatc cgtaaaatac
     3841 cttatatata taacgggaag atataaaacc taaaattatg ataacctgct gcagcttcta
     3901 catagaaaga gaagcgctca acaccaacct catttatcaa acgttggcca taaccccaaa
     3961 tctcttctga tagctgatca aaggttatat actttagctc atattgctgg ataatgttca
     4021 ttctaatcga ctccatttat tatcctggta aaattcgata tgacgtattt cgtcgtagtg
     4081 aattttgtct tttccaacgt agatccccag ctcatcacta cctaagatct tgccaatgat
     4141 atcttctgga tagtatcctt cagcgtttcg ttcttctttt tgaatagata ctgatctatt
     4201 ttttatgact gcagcgttca atacttccgc aatctcttca gtcgtcattt gttcttttgc
     4261 taaattttga ctggttcgtt gttcattgtc ggcatcaatt tcttcagtat gatcagataa
     4321 ataaaaccct gcccacttta aacgtccacg atcattgtaa tcaatcattt ttaattcccc
     4381 catttccgcc cgcatgacca ccaacaagtg tgcttctagt aatgcttcta gcgccttcta
     4441 acattgaaga agcatgtaca atcgacgtaa aaccatattt ttgccgaatc ttgtcaataa
     4501 ttttatctaa attttcttcg tcaatttgct tttgtggatc actaaataaa tccagctgaa
     4561 gcgaatcggt gtacacaagc ttgctatagg ttattccaac atgccttact tcttgtcctt
     4621 cataatactt atcaaacaag aaaagacaat aattctgcaa ttcctttgta ttatctgtga
     4681 tagggatctt catctggtgt gaaaaccctg gtctcgtttc taagatcgac gtgccaatgt
     4741 gtaaatggac acaacctgtt ttacagttat gctgcctaat gcgtatcgct acttgttccg
     4801 ccatttcttt taccaccagc tcgatttcat ttcttctagt ataatctcgt ggtaacactt
     4861 gtgaatttcc atatgatttt tcctttgtcg gttctggtgg gattgctata tctgttcgat
     4921 cgatcccatt agcatggaag tacagctgta agccaatcac acctagtcta ttcttaatcg
     4981 tataaggatt ccagttagca agatctctaa tgctcataat ccccatctga ttgagtcttt
     5041 ttttcattcg agagccaatt ccccaaaagt ccgtcatttc aggaatattc catacttttt
     5101 caggaacgtt ttcgtacgtc cattccgcaa caaatccttc attatgcttt gcttcgttgt
     5161 ctaaggctag tttagccaaa aggggattat ctccgacacc tactgtagca atcaatccta
     5221 gctcttcttt aatattctcc tggatcattt gagccagctt ctttctccgt tgacttcgtg
     5281 ttccttcagt cgtaaaaaga ttcagtgatc gggtcacttt taagattgat tcatcgatcg
     5341 agtaaatcag tagatcttca tcagccacat atcttctgaa aatattattt acctgcatat
     5401 ttcgcttgat atataggttc atacgtggtg gaacaacgtg tagtgtttta ggaaatggtt
     5461 gtggtaagtc acgtggtcta ctcacatttg taataccata ccgctttttt gcttcaggag
     5521 aagaagctaa tatcaatcct gaaccagtat tatcagctcg actcataaca acaagttctg
     5581 ttgttaatgg atctaaattt ctttctatac attcgacact tgcgtaaaag cttttcatat
     5641 cgattaaaaa gtaatcattt actggctctt ttgaataatc catcataaat aacactccat
     5701 tctttcacat tatacaaaca tatgttcgat attgtaaaga agaaatagct aaatacataa
     5761 aaaaagaagc ataggtatgc tatcaccctg cttcttcttt tgatttgtat gatcaattta
     5821 ttctatcact ttactaatta atttgaaaat ctaataaaaa ctctcactct aaaagagtga
     5881 gagactgcaa attagagtac ggactttttt ggagttccgt tctttaatat taacaaatga
     5941 ataaaatcct tacaacattt gagaataagt tatcagtaga cacttacaaa aaattctcat
     6001 actatttcct ccatcattag tttttgtcta tataaggttt accctaaata gacactggta
     6061 taacattgaa aaatatggct tcctcgtgta tgattagggt gtattttaaa tagttgcaga
     6121 aagtaggtaa aagttttgag agttgcatac atacgtgtct catcgatcga tcaaaatgaa
     6181 caacgccaga ttgaagaaat gaaaaaattc ggtgcagaga gaattttcat cgagaaacaa
     6241 tctggcgcaa cgattactca tcgacctgtt ttccaagaag cattagatta cgtccgagat
     6301 caagatattt ttattgtgga agctattgat cgacttggaa gaaattatga cgagatcatc
     6361 gcttcagtca attacttgaa gaagaaaaat gtgaagctga tcattactag tttaccgatc
     6421 atggctgaag caattggcaa tcctctcctg gataaattca ttaaggatct gatcatccaa
     6481 attttggcca tgatcgctga acaagaacgg actgaatcga aacgtcgcca ggcacaagga
     6541 attaagatcg caaaagcaaa cggcgtctac aaagggcggc ccaaattata cagtgcagac
     6601 gcaaaagatc cccaacgtcg ccttgtttac aaaagcatcg ttgaagatct taaaaatggt
     6661 gttgcgattg ctaagattgc taaagactac aatgttacga gacaaaccgt gtatagaata
     6721 aaaaaagaca gcatggtcaa tgacaaatag ttgatcatgc tgcttttatt atcttctcat
     6781 ctttaatttc ctacatataa ttactatcta taaaattaag tcttactcag aacagataaa
     6841 gcataaaatt ccaacaaacc attttttact taactaaata aagagtctgt taatataatc
     6901 ttcacttatt tacgttaatc ccctactgta tttgttggtt ccttatctat attttctaca
     6961 aaatccttat tatcttgtga tttagcttcg tataattctt tgtatgtcga cacattggtt
     7021 atactaattc ctatagctag ttcttgagac tctacttttc tctctagctc ctttgacaaa
     7081 gtgatttgag atttatttgg ttccgttgga gtcaattcta tagattcttc ataaaaatct
     7141 ctatacataa agctatcatt aattttataa aaaaaattaa atgtttttag cactgccata
     7201 taacattcaa aacattcttc tatagttggt ttacttcctt gaggagaatg aaccaattta
     7261 ttacgttttt ctatgaaatt tttaatttcc tttcttattt ctttcggaaa ctcaatatta
     7321 aagtaccctt taaacatgct ccctaataat ttgtctacag ggggagattg taaattctca
     7381 aatattagag ttaaatcagg tttctcttgt tgataaaatt cctttacccc tatttcacag
     7441 gctgtaatta tcgaaataaa cctatctcta gtatttgata tatttttact tcttttaagg
     7501 agtataagag aaaaggggat actttcgtgt ccatgcatgt aattaactaa ttgttgagct
     7561 tcagaagtta tatctctaat tatatagtta acggaattag gaagtgtctt taactctaat
     7621 gaaatttcta tccaattact atcttctaat ctgtatatgc tcgaagtata atttgattcg
     7681 tcagcaagca tcgctacatc ccaaacactg atcataaaat cttttaaacg ttcaatcatt
     7741 tcagcaatat tgctaaaatc agctaatttt tctttagaat aaaaaaaaac atttatatca
     7801 gaatcaataa attctatttt tttattacta tatcgtcatc attatcttga ccatttatgt
     7861 ttattgttcc ctgagtgggg agtacttctt tcaaattcaa gttaaattta tattccatta
     7921 cttctcctcc aatatataaa aattacttaa ctgtatccaa atttaatatg taaataaatc
     7981 attaattttt agctaaattc tatgagaagt ttatttttgt tgtttttgct aattaactaa
     8041 aactagtaca aaaaatttaa aatagattat taaacctatt gcttattcta ttcagtcata
     8101 tatataagcg tcatccttta gtaactaaca tcccacacta caatagtatt ataacatatc
     8161 tattcaactt caaattgttg aaatttaagc catttatatt tacttttcct caactttaga
     8221 aatgatttta ctaaccttat atccgtaata cgcgtaacaa acacattcat taataacaat
     8281 caacactaag ctatattagc tttttaaatg actagggtaa ataaaataag gatcaaaaac
     8341 ttttaagttt ctgacccttt ccttacttcc gattggttaa tagtgatctt ttgcagaata
     8401 aataaatatc gtttcatttt caactctata tatcagtcta tgttcatctg taattcttct
     8461 ggaccatttt ccagataaat catgctttaa tggctcaggt tttcctaatc cagcaaaggg
     8521 ggaacgatcg atatctttta ttaacttgtt aatctttttt atattgcttt tgtttccttg
     8581 ctcatgccaa taaagataat catcccaagc atcatcagac caagccttaa tcatcagatt
     8641 caacctcgat taagtcatgt gttttaaatg cacctttgga gaattgttca tctcctcgac
     8701 gaattttttc catgacgtaa ttattagaaa gtgttctcaa cgtttcttgc atagaatcat
     8761 aatctctttt tgataataca acaactgtat cttctacatc tttacttgtt acaataagtg
     8821 tttcagcatc ctcattaact tgtttcatat aactacgtaa attttggcgg aaatttgaat
     8881 aagctactgc ttccattcct ttcacccctt atttatttct ataaaacaat tgtacattat
     8941 attgtacaat taagcaaatg attaatattc taacttctta taattaaaag tgctatcact
     9001 tttagttttt aggataaaaa gtgacgtcac tttttgagtg ctggtacctc aaatcctgct
     9061 ttttctt
//
LOCUS       DACEUZ010000100         8855 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.100, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000100 DACEUZ010000000
VERSION     DACEUZ010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8855
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.100"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(209..607)
                     /locus_tag="IXG44_002046"
     CDS             complement(209..607)
                     /locus_tag="IXG44_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAQ1658753.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
     gene            complement(604..840)
                     /locus_tag="IXG44_002047"
     CDS             complement(604..840)
                     /locus_tag="IXG44_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ1658754.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            complement(1000..1317)
                     /locus_tag="IXG44_002048"
     CDS             complement(1000..1317)
                     /locus_tag="IXG44_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1658755.1"
                     /translation="MKKVHSFLNYLMGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAY
                     SPTLILSTLTAGAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQ
                     YFG"
     gene            complement(1455..3683)
                     /gene="topB"
                     /locus_tag="IXG44_002049"
     CDS             complement(1455..3683)
                     /gene="topB"
                     /locus_tag="IXG44_002049"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAQ1658756.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            complement(3857..4267)
                     /locus_tag="IXG44_002050"
     CDS             complement(3857..4267)
                     /locus_tag="IXG44_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAQ1658757.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            complement(4384..5547)
                     /locus_tag="IXG44_002051"
     CDS             complement(4384..5547)
                     /locus_tag="IXG44_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAQ1658758.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            complement(5725..6579)
                     /gene="aroE"
                     /locus_tag="IXG44_002052"
     CDS             complement(5725..6579)
                     /gene="aroE"
                     /locus_tag="IXG44_002052"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HAQ1658759.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            complement(6858..7628)
                     /locus_tag="IXG44_002053"
     CDS             complement(6858..7628)
                     /locus_tag="IXG44_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1658760.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            complement(7631..8512)
                     /locus_tag="IXG44_002054"
     CDS             complement(7631..8512)
                     /locus_tag="IXG44_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1658761.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            complement(8509..>8855)
                     /locus_tag="IXG44_002055"
     CDS             complement(8509..>8855)
                     /locus_tag="IXG44_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1658762.1"
                     /translation="NTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQYQL
                     GLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKIITD
                     DSQQETSKEDDK"
BASE COUNT         2410 a         1874 c         1485 g         3086 t
ORIGIN      
        1 aaaatatata aaaaatcagc ccactagttc taaaccacta tttcctacta ttcactcttt
       61 tgtattcctt ttaaatcaat tatttcaaaa ttaatccata ttttccatat ttttatgaaa
      121 aaactatttt gtatgattaa acataaaaaa atcagcatat tccttaatga catgctgatc
      181 taattgagaa ataaagtttt taattcgttc attttattgc ggtttgtcgt aaataactgg
      241 ttatcttgga ctttagtgaa tcgaaatcca atgaactagt ggtaatcatt atgataaaat
      301 caacagcttc ttgacgagaa aaagcaactg gacaattatt caattgaaga aacaacaaca
      361 tggcaacaag agcagttcgt ttattgccat tatgaaaagg atgtttttta gctaaattaa
      421 ttgctaaaat cgccgctttt tcatagattt caggatagag ttcttgattg aatactgctt
      481 gtgaaggctg tttgacggcc atatctaatg catttgcatc ttttacaccg atcatttcgc
      541 ctggcgagtg acgtaaaata actttcgcgt tgattttgat aatttcttta gtcgaaagat
      601 acctcataaa tcaaccaaat cttttaacgc gtcttcatat tgattcatga tcgaatcaac
      661 taatgaatca atatcaggtt cttcttgtgc tttgatcatt cgtacagaac catcagattg
      721 aaaaacataa ctgattgcgt cacctgtctg cacacccatg aattctttta cttctggtgg
      781 gatagttgtt acatcgctat tgccagattt tctaatttta tactttttca tttttttcat
      841 atttcgccac cctctatacc attctcattc tatagtaagt acattatatc caagtatata
      901 cttttaatca atgtatatac ttttattttg ctttttctta cgaaaaaatg acaaagaaaa
      961 aagtttgtga catgactttt gtcacaaact ctcgtattct taaccaaaat attgatgaaa
     1021 tacttgtgta ataactggta gaaaataaat aatgatcata gccgctacca ccaccaacaa
     1081 ttgttttcgc tgtttctttc gctttagcat gcttgtcgtt ttatgaaaaa aataagtagc
     1141 gcttttagct cctgcagtca aagtcgataa aatcaacgtt ggtgaatatg ctgtttgatt
     1201 gtgcgtcaat gacaacaact tcgtcccgta atcaggatac tttagaaatg cttggtacac
     1261 agccacaatc gtgattgctc ccataagata atttaaaaat gaatgcactt ttttcaaata
     1321 aaaaaactcc ttgctatcta tataaaatca gcctacccca acgtttttgc ttgattcact
     1381 gatatttttt agttttattg gtatttatat tctcactttt cggaataaat attccgctat
     1441 ttcattgttt tcatttactc tacttcgttt tcattatttt ttcttgatgt ttctttgttt
     1501 ccatcaactg cttttgttcc actgttttcg atccatggaa aagaaaaacc gattgtcata
     1561 tcttctttta atactaaaac tgcatcgaat ttcccttttt taccagtaaa acctttgaca
     1621 actttacttg tctttccttt tgaaagcaaa tctctgagat tcgttttagt cagcttcttt
     1681 ttagctactg ttggccacaa tttgaagtcg cattccggat tcaagcatgt agcgatttta
     1741 ggataaagca tgactattcc ctttttacat tttggacatc ttcctaattg atcgtttttc
     1801 ttctccatcg cttttatctg acgtgtcccg ctaatctcat ctgaaaagtc taccttgtcg
     1861 atttgattag gaacttctgc caataaatgt aggatgaatt ttttgatatt ctctaaaaaa
     1921 acagatggtg tcccctcttg tttaccaatt tttctcaagt acccttccca gcgcgcagtc
     1981 atttctgcgc tcgtcagtaa tggttcattg gctactgcct tacaaagagt gattcctttt
     2041 ggcgtaacag ttatttcgtt tttattgact tcgatgtaat gcttctgctt aagattttca
     2101 atgatattgg ctcgtgtagc ttctgtccct attccttcta cttctttgag aatttcttgc
     2161 gcttcatcat tatccagcgt ttttcccgca gttttcattg ctgtgatcaa cgttccttcg
     2221 gtatagggtt ttggaggttg tgtttctttc tccatacttt tcagttctac ttctgcttct
     2281 tcattttcta cgataggagg aagagtctga gcttctttct tttttgactt gatgcgtaag
     2341 atttcctgcc aaccttgctg taatgggatc tttccgatgc tttttagctt taattgctgt
     2401 acgcccgtca ttacggtcgt ctcttcgtag atataatctg cagcaaacat agccacagta
     2461 gtgcgcaaaa cttgcaaata gattgcttgc tgtaatttgg aaagcttcgc aaagcgtgca
     2521 ggagtcggaa tcgtctttgt cggaataatt gcatgatgtt cttgaacttt tttcgcatcg
     2581 acatatcgtt tctttgcttt gatctcagtt gtctgtactt cttctattcc taaaaatttt
     2641 ttataatttt gcagatgttt aaccagatac tgatgctcat tttctgtaat gtaaaaggaa
     2701 tccgttcttg gatagcttag aaacttgctt tcgtacaatc cttgagcagc atcaagtgta
     2761 gctttggcac ttgctttcat tagctgattc atctttgtct gcaaactaga tagcgaaaat
     2821 aaacgtggac tatgcaacct cttttccttt ttcgtaactt caagaatccg tccgttttgc
     2881 ttacctaaat gcgctccaag tttttctatt tccttcgtta actcttcttg ggttttaaat
     2941 gctttttttg gtacgatttt tccagaaaac tgccctttgt ttgtcgtaat taaaccttct
     3001 ccttcaaagt acgtttcttt tttgaagtgt ttgatttctt cttgcagctg ataaatcata
     3061 tagagtgtcg gtgtttgtac acgaccaaga gaaaaactgc catcaatccc ttttcgttga
     3121 agtagcaagc tatataatgg acttccattc attccaatca gccaatcagc gttctgcctt
     3181 gcttgcgctt ctttatattt tggaaaatag ttctttccag gttggagatt cttaaaacct
     3241 tcatagattg cttctttttc aagagaattg atccataaac gtaaatagtt cttttcctta
     3301 gaaaatgcgt ttgcttgtcg aataatcgac cacgcgatgg cttcaccttc tcgatcacca
     3361 tctgttgcaa taatgatggt atcggctgac tgcaattctc gttttacgat cgaaaattgt
     3421 gcacgtttgt ccattggaac ttcgtaacgg aaggtttctg ggaaaatagg caaattttct
     3481 aaagaccatt ttgcccaacg ctcttcataa gctcctggag caaccatatc taccaaatgt
     3541 ccaaaaccat aagtgatggt tacttcacca gcaaacaatg gatcgctgat ttcataatac
     3601 ccttcatgcc gcttttgctg ttggaaggtt tcggcatagg cttttgcttg tgaaggtttt
     3661 tcagctagta tcaccgtctt catcttctga cctccgttac ttaatttttt gcggacttct
     3721 ctattatgga acatattttc gcatctggca ataaaaaaaa gataccgtat agtcgaaata
     3781 gacacaaaaa catcaagcaa acaaacgatg atccgtttgc tgcttgatgt ttccagaaaa
     3841 tattcatctt tagctgtcac tcttggttaa tctgccagtt gatgccaaat cgatcagtta
     3901 ccatcccata caactgagcc cacggaacag catccagtgg catctcaatc ttaccaccct
     3961 cagctaagct ttcaaatagg cgcttcgttt cttcttcatt tcttgtctgg atgactaaag
     4021 aaacattatt tcccacaact aattcaggac caattccttc tggcgtatct gcaaacataa
     4081 ctaatgtatc ataaatgttc agccgtgcat tcgtgattag attttttgct tcttctggca
     4141 agttcatttg ttctattcca ggcatttcac cgtatgtggt gatacctgat gcttcagtct
     4201 gaaaaacttt ttcataaaaa gcgatggctt cacgcgactc atttttgaag ttcagataga
     4261 tatccaatgt aaacagctcc tctcttggtt aaatctacca taagaaagcg ttttttacga
     4321 gtgaatcgtt tgattgattc atgacaaaag ccttgcgcag cttcttcttg atctttttta
     4381 tgatcattta ttcaaaaaat ctgtaatagc agattgattc atttgattat caatttcaag
     4441 aatactgcca gcataatcac tttgcccata attccacgaa ccgtctactg gcacacttat
     4501 tcgatcaatg ccaccagctc ctcgtgcaat cgaaaagagg tttttcgtca agaaccaagc
     4561 agtgacatct gtggatgtat atcctaaagc tttaccagct gcataaggtg cacggatcag
     4621 attcaatgga ttcttcaatt ggctgaaaat agcgttcatt acttgctgtt ggcgtctgac
     4681 acggccaaag tctccttgtt catccattcg gaaacgtgcg tactgcaaaa gaacatgtcc
     4741 atccatcttc tggacccctt ttttaatatc tacaccatcc agattcagat ctttttctgc
     4801 atcgatcttc actccgttca tgaataaggc atcgatcact ttttcaaacg atttgaaatc
     4861 gactttcgca tagtattgac aatcgattcc aaaattttct actaaagttt gacgcactag
     4921 atcggctcct ccgtatgcat aagctgaatt gatcttattt tgtcctaccc ctgggatcgt
     4981 aacgaagctg tcacgcataa aagaaatcag tttcggctta tgggaaggac catccaactg
     5041 taagaccatg atcgtatcgg ctcgcccggc atcttctccg cgtgtatcac ttccaaggat
     5101 caaaatatta tgcgcaccat tcgtcgcttt ttgcccatga aaggtttctg aagtcgctgc
     5161 ctctaagctt gtgtcatgtt cagctacata tttaccaata aaaaatgaga tacctgaata
     5221 aaggatcaaa acaagcaaaa tcgatagaat gatttttttc cacgatcgct ttcttcgagg
     5281 ttttaacggt ttgttcttct tttgccgatt gaaaaatttt ttattttttt gttgttcttc
     5341 tgctcttggt gcatctctgt atatctgttg ctcgtattgg ttcaaatgtt caaatgaatg
     5401 ctgctctctt tcataaatac gggtagcatc ttcctcattt tccatacgcg ttcgttttcg
     5461 ttctcttcta gcaaaaatac tccccgtatc ccgcttcaga ggtttcgcct tcttatggat
     5521 gtccttatag cgatccatcc gactcattgt ggttcctcct cgtttcttac tgataaataa
     5581 aggatacgat aaccaatcga caaaagccta tcattttttg ataatttaat aaaaaataac
     5641 gaaataaatg cttcgataca taaaggatta cttttcttga gaccgtttat gcgagaataa
     5701 aagatcttta tctattatcc ttttttaatc aaacagcttt tcaatcacat ggatcggcat
     5761 cttttccttt gtccataatt caaacgcgat tgctccttgg tagatcaaca tgccaagtcc
     5821 attgtatcct tttgctccta ctttttttgc ttcttttaat aaacgtgttt ctctaggtgc
     5881 ataaatcaaa tcaaaaaccg ctagattctc atgcatcatg cttatatcta agagcggtga
     5941 ctgattgatt ttctttccca tcccgatatc tgttgcattg actagaagac agctttcttt
     6001 caagtctttt cttaattgat gcgtatcggc aaagtcacga acttttatct tacaagaagt
     6061 tcgatagctg agttgttcga atgtttcttt tacttgagcg aatgtttggt tgtttcgttt
     6121 ataaactacg atttcctcag cgccgtctaa cgcagcttga acagtgactg cctttgctgc
     6181 acctccagct cccagtagag tcattttttt ctttttgatc gaaatcccag cttcttgcac
     6241 ggattgcatg aagcccgcac catcggtatt atagccaatc aaacgaccct tttcgtgcac
     6301 gatcgtattc actgctccga tcaattgcgc ttcctggctt acctcatcca gataaggaat
     6361 aaccgctgtt ttattcggca tcgaaagatt tacacctaac atatcaaaag ttcgaatact
     6421 ttcgactgct tgcttcaaat tcattggatt tacttggaaa gctaaataca ccgcatcgat
     6481 gttgtttgct tcaaaagcag tattgtgcat caatggggat aaactgtgag aagccggatc
     6541 agcaaagagc ccagctaatt tggtttgtcc agaaatcatc ttattcctcc tttgttctta
     6601 cttcaataaa aaaagccact tagcgtttga tacgaaaaag tacgaaacgt ctaggtggca
     6661 aagtagcaaa ttcttttctc atcgtttact tgtcagagca aagaatattc tatttaattg
     6721 ttaaaatcat tgactacgtg atcctacaaa acacccaaat atgtttaatt gtttattgtt
     6781 ttaagtatag gatgaatcaa aggaagatgt caatgagttt tcaagtcctt ctactgaaag
     6841 gaatttttct gtcgaaatca atccactact tcttcaaaga actgcaaaag gtcgcccatt
     6901 gtcaaatgtt gcttttcttt ttcagaaata tcttgaacga ttttcccttt ttggagtacg
     6961 atcagacgat ttccgtattt caaggcgtct tccattcgat gcgtgatcat gagacaagtc
     7021 aatttttgtt cattgatccg ctgatttgtt aatcgcatta atgctttagc tgttttagga
     7081 tctaaagctg cagtatgttc atctaataat agtaactcag gagctgtgat cgtcgccatc
     7141 agcaagctta atgcttggcg ttgaccacca gataattcac ccgctggagt atccaagtgt
     7201 tgctctaggc cattaccgac ttcttgacat agtctgaaaa aatcttcttt ttgatttttc
     7261 aagttacgta aacggaattt ccgtttttga cctctttttt gtgcaagtag caaattttct
     7321 gctactgtca tccgaggagc cgttcccatc ttggggtctt ggaaaactcg agaaaggaat
     7381 cgtgctcttt tttcttctga atcttttgtg atcagctgat ctttaaaaga gatcgatcct
     7441 tgactgagtt gaagcgtacc agcgatcgta ttgaacagtg tgctttttcc agcaccattc
     7501 ccgccaagga cagtgatgaa gtccccttct ctgattgtaa ggttaatttc atccaatagt
     7561 actttctctt cattcattcc attgtttacg acttttctgc catttttgat tgtcaatata
     7621 tcagtcatta ctatgcctcc ttctcaaaac ccgtttttaa gttcagtgct ttcttcaatt
     7681 gcgggatcat cagacaaata gctaagatga tagcggagaa caacttcaga taagtcgtgt
     7741 caaaccctag tttgattact actaaaatca atagctgata aatgatactg ccgacaacaa
     7801 tagcaatcaa tctttctgct agcgtcaatt cttggaagat cacttcaccg atgatgatgg
     7861 aagcaagacc gatgacgatt acgccaatcc ctttatttac atctgcatat ccgtcgttct
     7921 gggcaatcag cgcaccggat aatgcaatga caccgttcga caaagttaaa cctaaaattt
     7981 tcatacgatc ggtttttatc cctaacgacc gagccatcgt ttcgttgtct cctgtcgcaa
     8041 tatagccttg acccaaatct gtattgaaga agaatagcag tacagcaatc aagatgccca
     8101 aagagatgaa gccaagaagg actgtgtcaa agtaatcagg taacccaaag cgatggaagc
     8161 tgtcttgtag aatcggatga ttcaataaag aaagatttgg tgttcgcatc acaaacaaaa
     8221 taactgagtt caatcctgac atcactaaaa tccctgctaa aataaccggt atctttcctt
     8281 ttgtataaag taaccccgta acgagtcctg ctatcatgcc agcacctacg cctaataatg
     8341 tagctacaat cggcgctacc ccatgagtaa tagctgtcac acagacagca gcacctaatg
     8401 ggaaggaccc ttctgtcgtc atatcaggaa agtctaagat ccgataagtc atgaaaatcc
     8461 ctaatcctaa tattgcccaa agcatgcctt gtgaaatagc tgatacgatc atttgtcatc
     8521 ctccttagat gtttcttgct gcgagtcatc tgtaatgatt ttggcttttt ccttcatcga
     8581 ttggggaaca atgattccta agcgatcagc ttgagattga ttgatcactg tatccccttg
     8641 atcaaatgta taaacaggcg tcgtttccgg tttttctttt ccactcgcaa gttctgccgc
     8701 catcttaccg ctttgaagcc ctaattgata ttgattgatc ccgattgttg ctagtccacc
     8761 ttgttccacc attgtatcca cagatggaat gacggggata tttgctttgt ttgcttcttt
     8821 taccactgtt tgcatcgcat tggcaatggt attat
//
LOCUS       DACEUZ010000101         8610 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.101, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000101 DACEUZ010000000
VERSION     DACEUZ010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8610
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.101"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(<1..1467)
                     /locus_tag="IXG44_002056"
     CDS             complement(<1..1467)
                     /locus_tag="IXG44_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAQ1658763.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDG"
     gene            complement(1733..2608)
                     /locus_tag="IXG44_002057"
     CDS             complement(1733..2608)
                     /locus_tag="IXG44_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ1658764.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            complement(2717..4486)
                     /gene="aspS"
                     /locus_tag="IXG44_002058"
     CDS             complement(2717..4486)
                     /gene="aspS"
                     /locus_tag="IXG44_002058"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HAQ1658765.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            complement(4501..5793)
                     /locus_tag="IXG44_002059"
     CDS             complement(4501..5793)
                     /locus_tag="IXG44_002059"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HAQ1658766.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            6187..7134
                     /locus_tag="IXG44_002060"
     CDS             6187..7134
                     /locus_tag="IXG44_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAQ1658767.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            complement(7223..8482)
                     /locus_tag="IXG44_002061"
     CDS             complement(7223..8482)
                     /locus_tag="IXG44_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HAQ1658768.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
BASE COUNT         2513 a         1850 c         1534 g         2713 t
ORIGIN      
        1 tccatctacc actacttttg gataaagttc ttgtagatcc gttgcagcat ccaacataac
       61 tgaatcacca aatgccgtta gttctaattt ttgtgcctta ttcccttgtg ccttggacaa
      121 ctcgtactta tcaaggatcg cttggtcaac cgaactagtg gtgttttctg aattgtttct
      181 ggtttcttct gctaattttt tactttcagc gatttttgcc tgcagttgtt tttgatcggc
      241 tgttacagcg tttttcggtg ccgtaacgaa accaacgagt gcaattgcta caagtaatgt
      301 gcctggaaca acccatggtt tgctcttact gatgatcggt ttgctgaacc atcctttcat
      361 cacttggaat gtacgaccat aatcaaaacg tgctagcgga cgctccacat atcgataaga
      421 aagttcactg atgattagaa tcaatgtgat ttccactaaa gtatgcaaca aaacatgatc
      481 ggcaatatca gtcacctttg cttcataaaa aatcataact ggaaactgat acagataaat
      541 gccatagctt cgtttgccaa tccaagtaaa tactggattt gtcagccatc gattcaaact
      601 tgctccgggg tgtgctgtca ctgcaactag cactacgcat aacagactca ctaggagcat
      661 tcctccgtaa tagatgaaat tgtaatgatc atctaaaaag aagaagcaaa gaatcagtgc
      721 caaaagcgag attcctccag cgatattcag aatatttttc gcttgtttcg ggatgttttt
      781 ctttaaacgc gtacttggcc aaacaaacgc aagcgcactt cccaaccata tagaaaacag
      841 acgagtgtcg gtaccgtaat aaacacgggt tggatcgcct cctggtgtat acaagatcat
      901 catcaagatg gcggagagga aggatgtacc taacacagta taaaagatcc attttctttt
      961 acgaacaaat accatcaaca atacaaacaa gatcggccaa accaaatagt tttgaccttc
     1021 tactgctaaa gaccagatat gcgtaaacgg tgactcatta gcaaatcgat cgaaataaga
     1081 taaaccgtta ttgatttgcc accagttgtt gacatacagt aagctgctga ccacaactcc
     1141 tcgaagattg tttaacagcc ctcgctgaaa caaagtgata taagccgaag ctgtcacaag
     1201 cataaaaaca agtgccggat agagtctctt cattcttctg acataaaatt gccaaatatt
     1261 gatttttcca ttttgttccc attcctgccg cagcagatcc gtgatcaaat aaccagatac
     1321 gacgaaaaaa atcggaacgc ccaaatagcc gcctttcata ctagttggca gaaggtgata
     1381 gaagataaca cccaccacag ctaatgaacg gagcccatca aacccagtga tgtagcggct
     1441 gttttttaac cttttttgtt tttccattca atattccaac ttttcataaa cttttttcat
     1501 tcgtaatata cgggtattaa gtgaaaatag caagtagaat cgtgtccttt ctctactatt
     1561 tttttctatc atcccataaa tttaaaaaaa taaacatgct ttttttcaac tttaagaaaa
     1621 tttcaaataa aaaagcagtt tttctttgct gattctacta tggtagaaaa aaactgcaga
     1681 caaacgaacg tctgtctaca gcagtatttc atgaattttt gtcaaactcg atctaaatgc
     1741 catcctcata gaaacgacca agtatctgga cgttttctgc aatacttaca aaagcttgtg
     1801 gatcagaagc tttcattgct tctctaagta gaggcagttc gtatcgtgta acgatcgtca
     1861 tcaatactgt ctgcttatca tgcttataag caccttctgc ttcattgatg atcgtgatcc
     1921 ctctacgcat acagttttgg atttcattta ctactttttc tggatgtttc gttacgatca
     1981 ttacctgcat cttcttttgt ttagtataaa cgacatcagt tacttttcca ctgacgaaaa
     2041 tagacaacgt actgtaaaac atatattgcc agccaaatag aattccggca acaaaaacga
     2101 tcaatgcatt gaagtagatc gaaatagaac caatcgactt tcctgttttt ttacgaatcg
     2161 tgatactcac gatatctaga ccgcccgatg ataggccgtt tctcaacgct agcccaatac
     2221 ctagtcccat cacagccccc ccaaacaacg cacagatgat tggatcggta gataggactg
     2281 tttccggtaa aatatggata caaagggagg tcaaagtgac tgtgataaaa gtgaaaattg
     2341 taaacttctt cccgattttt aaccacgcca atacaaacaa aggtatattc agtaaataca
     2401 aagttgttga taccggtact tcgaatccta tcagtgattt tgacaaagtc gtcaaaatct
     2461 gagctagacc agtaatacca cttgaataaa tatgtcctgg ctgataaaag aaattcaatg
     2521 cgactgatgc gatcaacgca taaacgatag ataccgaaag cttggtcgtg taatcatgat
     2581 aagcccaatt ttcgaatgtt tttttcatgg aaaagccccg ctttatatgt tatggcttca
     2641 ttataaagtg tttttcaatt aaatcaaggt tgtcaaaaga agcgtattcg acaaatcctt
     2701 tttacctttt tatcggctat tcttctatat tcgttacatc aatattcaat tcgaacaatt
     2761 gtaaaggcga aaccatactt ggtgcatcac tcatcacatc tgctgctttt ccgtttttag
     2821 gaaaggcaat gacttcacga atattatctt ctccggctaa taacattgca aaacggtcca
     2881 atcctaatgc aataccgcca tgtggaggga atccgtaatc taaagctgtt aacaagaaac
     2941 caaattgttc ttccatttgt tcttttgtaa agcctagagt ttctagtact ttttcttgaa
     3001 gctcacgagt atgaatacgg atagaacctc cgcctagttc ataaccgttc aagacgatat
     3061 cataagcttc tgcatagact tttgcagggt cttcagccaa taacgggata tcttcttctt
     3121 ttggcatcgt gaatggatgg tgagcagaga catatcgacc ttcttcttct gaaaattcaa
     3181 attgtggcca atcagttacc cataagaaat tgaatttcga ttcatcaatt agtcctaatt
     3241 cttttcctaa acgtgtccgg atagcaccta atgcagcagc gacgatttca cttttatctg
     3301 cgccaaacat cagcaagtca cctggttttg catctgttgc tttaatgatt gcttctgttg
     3361 cttctcccat gaatttagca attggacctt tcaatccgtc ttcttctact ttcagccatg
     3421 caagcccctt agctccgaat tgtcctacat attgacctaa ctgatccatg tcttttcgtg
     3481 agtagcgatc tgcagcgcct ttagcattca atgctttcac gacaccgccg ttttcaagag
     3541 ccatttggaa gactttgaat tctacttctt tgactgtatc gcttagatcg atcaattcca
     3601 tatcaaaacg tgtatccggt ttgtcgctgc cgtagcgagc cattgcttca tcataggtca
     3661 tacgaggaaa tggcaatgtg acttcgatac cgcgaacttc tttcattacc ttcgcaatca
     3721 atccctctgt gtaagtttgg atttcttctg ctgttaagaa cgttgtttca atatctactt
     3781 gcgtaaattc aggctgacgg tctccacgta aatcttcatc gcggaaacaa cgaacgattt
     3841 gataataacg gtcgaatcca gcattcatca gcaattgttt aaacaattga ggagattgcg
     3901 gcaacgcata gaaatgtcca gcatggacac gtgaaggaac taggtagtct cttgcgcctt
     3961 ctggtgtaga tttccctagg taaggtgttt cgatatcgag aaagtcatta tcatctaaat
     4021 aatgacgaat cgcttttgtt gtcgcatttc ttaatttgat gtttttagtc atttttggac
     4081 gacgcagatc caaataacga tatttcaaac gtatttcgtc actgattgct tgatcatctt
     4141 caatttgaaa tggcggggtt tttgcttgat tcaaaatcac gatttcactt gcaatcactt
     4201 cgaattcacc agtggccatt ttaggattta ctgcattttc tccgcgtctt ctaacttccc
     4261 cagtcacttc gatcacgtat tcactgcgac atttatccgc gatttcccat gcttctttgt
     4321 tgatttcagg attaaagacg atttgaacga ttccttcacg atcacgtagg tcgataaaga
     4381 ttactccccc aaggtctcgt cttttttgta cccatccttt taatgtaact gtttgttcta
     4441 attgttgttc ggaaactaat ccgcaataga tcgttctttg tgccatttat ttttttcctc
     4501 ctactcgaat aaagtcattt catcatagac ttcatcaaaa tgttcatata tctcttctaa
     4561 tggaaaactt ttttcttttc ggcttgccat tgctttgaca ttaatcgttt gactagcaag
     4621 ctcatcttca ccgatcacca atactaattt ggcattcgct ttatctgctg tcttaaattg
     4681 cgcttttgct ttacggttca tgaagtcgcg gtcagctgaa aaaccaaagt ttcgaatcgc
     4741 ttggaccact ttcaatgctt cgatatttgt ttcgtctccg agtgcgacca cgtaagcatc
     4801 caactcatta aatgcaggga taactacttc ctctgcctcc atagtcaaga gtaatcgctc
     4861 gatacccatt gcaaatccga agccaggtgt tgaaggacca cctagttctt ccactagatt
     4921 gtcataacgt ccgcctgcgc aaatcgttgc ttgtgccccc atttttggtg cgtcactcat
     4981 gatttcaaaa atcgtatgtg tgtaataatc tagtccgcga accatattac tgtcgatttc
     5041 ataaggaaca gccaatgcat ccagcatgga aacaacggta tcaaaatgtt tttgagcagg
     5101 ctcgcttaga taatctaaga tagatggagc ttctgccaca aatttttgat ctcgtttgtc
     5161 tttactgtcc aatacgcgta atggattttc atgaaggcgg cgtttagagt cttcactcag
     5221 ctctgattca aaaggtaaaa gatagtcgat caatgcttga cgataggctt gacgcgtttc
     5281 tttatcacct aatgaattga tgactagccg gatttgcgtg atccccaact gtttgaagaa
     5341 atcaagtgcc atcaccatgc tctccacatc agttgcaggg ttttctgatc caaatgcttc
     5401 tactccgatt tgatggaatt gacgtaaccg tcctttttga gggcgttcgt agcgaaacat
     5461 tggtcccata taaaaagttt tgaacggttt cgcatactct gggccgaaca atttatgctc
     5521 aacaaaagaa cgaacgattg gtgctgtacc ttctggtcga agcgtcacat ggcgttcccc
     5581 tttgtcataa aaatcataca tctcttttga aacgatatcc gttgtatccc ctacacttcg
     5641 ggatatcact tcatagtgtt caaaaatcgg tgtacgcatt tcattatact gataatctct
     5701 gaataataaa cgtgctgttt cttcgacaaa ctgccatttt tctgattcgc ctggcaaaat
     5761 atcatttgtt ccttttggtc tttggaaact catagttttc tctccctttc ttagctatgt
     5821 aaataaaaaa atcacccttg ttcatcatga acaagggtga atgaaattca tacacggtac
     5881 cacctaattt tgaaagattt ctctttcctc ttacgaataa cgctcgtacg cgggacggct
     5941 tttcactcgt ccatctccag agtgtcttta ggctaatgaa actttagatc attttcagcc
     6001 tcgatgatcc tctcttgtaa agtttcaaaa acttactcgc tctttcattg acattcgata
     6061 ttacttataa cctactaaaa accagtctca aagtcaagag atatctgact atttcccctc
     6121 acaaaaacgt tttagttcag ctttttggta ttttgtttta gactagacaa aaaggaggaa
     6181 tcagctatgg cagaaataag aatcggacat tcacaagtct acgcagaaca gcttggatta
     6241 ggagcaaatg ccgttggagg acataatctt tttgacggtt tggaagatga gacaggaaaa
     6301 caagtagttc gtacagcttt gaatagcggg atcaatttga tcgataccgc ttatgcttac
     6361 ggaaacggcc gttccgaaga actgattggc gaggtactga aggaaaaaga atatgatcgc
     6421 tcacgtgtcg tcatcgctac aaaagctgct catgtaccaa ataaagggcg aacatttgat
     6481 aattctcctg agtttctcaa acaatcggtg gaagatgcac taaaacgtct gcaaactgat
     6541 tacatcgaca ttttttatat ccactttccg gatgaaagca caccaaaaaa tgaatcagtt
     6601 gctacattgc acgaattaaa agaagcagga aaaatccgag cagtcggtgt gtcaaacttc
     6661 acgttagaac aattgaaaga agcgaatgca gatggatatg tcgatgtagt agaagataaa
     6721 tacagtttga tccatcggca ggctgaaaaa gaattattcc cttatcttga aaaaaacaaa
     6781 atcagttttg ttccctattt tcctcttgct tccggtttgc tgacaggaaa atatgaactt
     6841 ggtgaggaaa aacaatttgg tgaaggagat cctcgaaaaa gaaatccaga tttccaagga
     6901 gaacgtttca gagaaatttt aaccgctgtc gatgtacttc gccccattgc taaaagatac
     6961 caagccactc ctgcgcaact tgttttagct tggtacatga agaatccacg agtgtctgta
     7021 gttatacctg gagctaagcg tccagagcaa gtatctgaca atgtccaagc tttagatttg
     7081 catttatcca atgaagatta tcaaaccatt gatgaagcat ttcgtggatt ttgatcaatt
     7141 gaccttatca atttatagta atatgaaaaa aggaagatca gcactgtttg atcttccttt
     7201 ttttcgataa gtttatgcta atctaattct tgtaaagtag cgctcggcta ttcggataag
     7261 tcggaaactg attgaatgga atcgtccaat cttgttcagg tacagagaac tcaaacttat
     7321 ttactagctg atcggaaaac acacgtagac tatgaagagt gatccattct cctgcacagc
     7381 gatgcatttg tcgaaaattt cccccgcctt gtgcaatcat ctcatattct tctttatagg
     7441 aaatatcttt tgctttccct acataccgtt tgatcataaa ggaatctggc gcttcaactg
     7501 ttcgttcatc atggtctgtt ccatataaat ctaaaataac ccaactgcct tctggtatac
     7561 gataaccatc tacttctact tctttcagcg aaatcgcagg tagcattggg aagaaaggat
     7621 aatagcgtct catttcttga ataaacggat cttgtaacgt cgaaaagtca ttttttaatt
     7681 gctgatacaa atctggtcta ctgaaaagtg catgtcccat caatgccgcc cacacagtta
     7741 gagcaaccgt tggacggatg atgttcaata attcaacagc tgccacttcc agaggtaaaa
     7801 gctgaccatc taaatctgtg gcattggcaa aagcatataa tgccacattt tcttttccag
     7861 ccacaggatt ttgtcgagct tcttcaatca agccttgcgc ccatttttct gattttttgc
     7921 gattttcgac accttttatg tgatcaatcg gagaagtaaa cgttccgctg atcatagaga
     7981 tttgatattc tgctaatttt tcgacctcat ctttagaaat cgctgataaa ttgatacctg
     8041 cccactcgca aatagatgta aagaaaacca tcttacttaa atcaaatagt tcaaattgac
     8101 catgctgtgc ttcaagtgct tgtgtcagat tcttatctaa gatacgatga taatcttcca
     8161 ttctttccgg tgtcattaga tccataaaaa tatttttccg atggtgatga gcagctccat
     8221 ccaaagtctg tactccacct tgaccaaaaa gtgtcttcaa tactaatttt ggcatagcac
     8281 cttttcgttt gaaattcctt ggatcataaa atacttttgc tgctgatgat ccgtagatcg
     8341 tgatagcttc tttattgaaa atctttgctt tgacgacggg agcgtccgct tcatggcgta
     8401 actcctccaa catgttgtat cctttttgat acaatttttt taaatctgtg attttgatat
     8461 ctactactgg tacttctttc atcttcagca acctcctata aaaaataact tgtataattt
     8521 aagcttaact ttttttccga ctgctgacaa aagaagcgct ttttgcagac aaaaaagcca
     8581 cttaagttag agtttatcag tctaacttaa
//
LOCUS       DACEUZ010000102         8286 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.102, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000102 DACEUZ010000000
VERSION     DACEUZ010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8286
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.102"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            215..1408
                     /locus_tag="IXG44_002062"
     CDS             215..1408
                     /locus_tag="IXG44_002062"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HAQ1658769.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            1538..3010
                     /locus_tag="IXG44_002063"
     CDS             1538..3010
                     /locus_tag="IXG44_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1658770.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            3093..3881
                     /locus_tag="IXG44_002064"
     CDS             3093..3881
                     /locus_tag="IXG44_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HAQ1658771.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(3948..4616)
                     /locus_tag="IXG44_002065"
     CDS             complement(3948..4616)
                     /locus_tag="IXG44_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAQ1658772.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            4731..6443
                     /locus_tag="IXG44_002066"
     CDS             4731..6443
                     /locus_tag="IXG44_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1658773.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            6446..8218
                     /locus_tag="IXG44_002067"
     CDS             6446..8218
                     /locus_tag="IXG44_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1658774.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
BASE COUNT         2538 a         1387 c         1821 g         2540 t
ORIGIN      
        1 taaaaagctt agcagagtta tctttatgga tgattgtgct aagctttttt ggacagttct
       61 aatgtaaaat atttttatat ttttaagcaa aaaaatgaaa aaatacgagc agaaaactgt
      121 tttatctgct atacttggtt cgttgcaagt tcccgaaagg atccgcagga cggagatgcc
      181 ttgtaaccga atttaactag ggggaatcat tataatggta gaaagacact tatttacatc
      241 agaatctgtt tctgaaggac atccagataa aattgctgac caaatcagtg atgcaatctt
      301 ggatgcaatt ttaaaacaag atccaacagc acgagtagcg tgtgaaacgt ctgtaacaac
      361 tggtcttgtt ttagtatttg gagaaatatc tacgacggca tatgtagata ttcaaaaagt
      421 cgtacgggaa acaataaaag aaattggtta tacacgtgca aaattcggat ttgatggaga
      481 tacagcagct gtattggttg cgattgatga acaatctcct gatattgctc aaggagttga
      541 tgaagcactt gagatccggg atgaagataa aaaagacgta ttagatgaga ttggtgctgg
      601 tgaccaaggt ttaatgtttg ggtttgctgt agatgaaaca ccagaattga tgcctttacc
      661 aatcgctttg agtcatcgtc tggtacgacg cttggcagat ttgcgtaaat caaatgaatt
      721 aacgtattta cgtccagatg caaaatctca agtaacggtt gaatatgatg atcaaggaca
      781 accggaacgc gtagatacaa tcgttatttc aacacagcat gatgatgcag tagataatga
      841 aacaattcgt catgatgtca tcgaaaaagt agtgaaggaa gttattccag ctgaattatt
      901 agatgatcaa acgaaatatt atatcaatcc gactggccga tttgtcattg gtggtcctca
      961 aggggatgcc ggattaacag gaagaaaaat cattgttgat acgtatggcg gttatgctcg
     1021 tcatggtggc ggtgcttttt ctggtaaaga tgcgacaaaa gttgaccgtt ctgccagcta
     1081 tgctgcacgt tatattgcta aaaacattgt tgcagcaggg cttgctcgca aagcagaagt
     1141 acagctagct tatgcgattg gtgttgctca acctgtttcg atctcaatca atactttcgg
     1201 tactggaacg gttccggaag aagaactgat tgctgcagtg agagaaaact tcgatcttcg
     1261 gccagcagga atcatcgaga tgcttgattt gcgtcgtccg atttacaaac aaacagctgc
     1321 ttacggtcat tttggtcgta cagatgtaga tttgccttgg gaacatacgg acaaagtaga
     1381 tgcgttgaaa gcaagtttag caaaataagt tatattcagc tggaacgatc gccaagtggt
     1441 ggttgttcca gcttttttta tggcagttaa cctcttgaaa aagatagtaa ttatttatct
     1501 atttttagat cctgtcataa tgaaagggag gaataagatg aaaaaagaaa caaatgttac
     1561 tttggtcaca gtgagtattt tcattgcaac gttcatgacg gctattgaag gtacaatcgt
     1621 aacaaccgct atgccaacga ttgtcggttc attacatgga atggaaatca tgaactgggt
     1681 attttcgatt tatttgctga caaacgctat gcttactccg atttacggaa aacttgcgga
     1741 taaagtggga cggaaaccaa tctttatttt tgggacatta ttgtttatta tcggttcgtt
     1801 attttgcggt ttatctcaaa gcatgattgg gttgattgct gctcgtgcga ttcaagggat
     1861 gggtgctggg gcaatgatgc cagtagcttt gacgatcatc ggcgatcttt attctattga
     1921 aaagcgagca aaagttttag gtttgaatag ttcagcctgg gggattgcca gtgtttttgg
     1981 accgcttgcg ggaggattca tcgttgaaac gatcagctgg cactggatct tttttatcaa
     2041 tgtgccgatc ggaattctat taattatttt gatttggcta tatttaaatg aaccaaaaat
     2101 catacatgag tctaaaccaa tggatatttt tggtagtttt agtttgatgg ccatgcttgt
     2161 ctcattgctg ttagggttcc aattattagg agacgaagga atgagtccgc ttgtttctgc
     2221 tttgctgcta ggtagtatcg taatgttagg tatttttgtt tttgccgaaa aaagagctaa
     2281 agatcctgtc atcatgatgc atttattcaa aaatccacta tttgttgtgg tcaacttggc
     2341 tgcagcattg gctagtggtt ttttgatggg aatcgatgtt tatatcccta tgtggatgca
     2401 aggtgtatta gggctgaatg cagcaattgg tgggctggta ttagcaccat tatcactagt
     2461 ctggatggtc ggttctttcc ttgcaagcaa gtggatgact caaatgaaaa ctcattgggt
     2521 cttgagaatt ggactactaa tcgcagctat tgggggcgct tggttgtatc ttatgccttt
     2581 cagaagtaac tatttatggt tttttgctat atcaacggtc atcggtatag gcttaggtgt
     2641 gaccatgact acctcgactg tcagtgcaca aaatagtgta tctcgtgacc agcttggtgt
     2701 agcaacttct tttaatacgt tagttcgaac aattggtcaa acagtcatgg tttctatttt
     2761 tggtttattg ataaatgggg taactaaacg agaacttgca gcagcaaatt taacaaatga
     2821 tgcagatatc atgaataaac tggtgaatcc acaaacggct aaatatattg atgcggatat
     2881 tttgttacca ttaagaaaaa ttctttatga tgggttacat tccgtttatc tagttggctt
     2941 gttactcgtg atcattgctt tggtcttaac gcttttcgtt catgaaaaaa gtggtaaaat
     3001 aaaacagtag tttcggttaa gatatgtacg tagccgaatc attcaaattt aaagttattc
     3061 aaaaataact tcttactaag gaaggcgaaa atatggcatt tattcaagca aatatttatt
     3121 cgaatgtgtt agaaatggaa gtcaatctaa atgtgatact accgcaaaca acaaaaaagg
     3181 tgattggcac cagttcagag caagagacaa cggatatacc tgttttatat ttacttcatg
     3241 gaatgggtgg caatcatagt gtatgggaaa gacgtacgtc gattgaacgt tatgtagcaa
     3301 actacggatt agctgtcatc atgccatcta ctgatcttgg ctggtacaca gatacagttt
     3361 atgacatgaa gtattggaca ttcattgcag aagagctacc tgagatttgt catgaattat
     3421 tcccgcaatt aacaagaaaa agagaaaaaa catatgccgc tggattatcg atgggcggat
     3481 atggcgcgtt aaaactaggc cttgcaaaat cagaaaattt cgcagcagta atctctcttt
     3541 caggcgctgt ttctgtagga aatcgaatgg atgatctttt gatggttcgt aaagcatctt
     3601 tttgggaagg gatctttgga ccattggatc aaatcgaagg ttcaaaaaat gatccgcaat
     3661 acttattgaa agaactagta aaaagcggaa aaccagttcc aagattttat atggcttgtg
     3721 gggaatctga ttttctttat caggcaaatc aggaaatggt atctgctatg caggaacaag
     3781 gcttggcggt cacctttgaa caaggacctg gcgatcataa ctggatcttc tgggaccaat
     3841 ggattcaacg tgcattggcg tggctaatgg aagaaaaata aaaaaagagt cttaaaaaga
     3901 tagctcctat agagatcaca aacatagtga tctctatagg agctttttta ctgctcgttt
     3961 tctcttcttt tattttgttg gcataagcct tcaatatagg tttgtgcact cgttagaaag
     4021 agtgcttttt cattcaattc tgcatgggac tgcatacttt ctttaaatcc ctctaattga
     4081 ttttcaacta cataattacg cataaataga actgtttctt tcaaaaattc atttccgtcc
     4141 ataatttttt gtcgaagctg ctgttcatgc tgctgatcca ttaactgtcc aatcaataaa
     4201 ttgtggagag aggcaatggc ctgatgactt tgggtgactg aaaatccttc acgaacaagt
     4261 aattggatca tttgctccaa atgttctaat cgactcggaa aagcgggcac agtattgatt
     4321 tctaattctg ccccacaagg gaaacgtgta taaaggtcat agtaattttc catatactgc
     4381 agcaactgtt cattccaagg caaagttgga tctggaagcg ctaattcttc ttccattgtt
     4441 tcagccattt tttgcaagag ctgttgtttg tttttgaaat accaatataa agcaggtgct
     4501 tgaacatgaa ttctgtttgc gatgtttcgc atggaaagct gcgaaagagc ttgtgtttca
     4561 gccaggatat caaaggcggc ctgaatgatg attgcttgag aaagctttat ttccatttta
     4621 tcaacctcgt gtattgcatt atctttttaa acatggtaaa ttgttagtcg gtctaatttt
     4681 acagtgttaa attagtcctg tcaatttaaa caaaggggtg taatcaacga atgaaattaa
     4741 tcttacgaca tgcgaaaaaa tataaagtag ctgtttttat ttccttgctg tccgtagcag
     4801 tcatggttgc agcagcatta tggcaaccta agttactaca gcaagtattg gaagctatta
     4861 ttacagaaga taatgatgaa atgaaaacaa ttggtattta cctgatcagt ctggctttgc
     4921 taggattagc cgcaggtgta acgaacacga ttttctctgc aaaagtagct caaggcgtaa
     4981 gtgcagatat ccgagaagaa gcatttcgaa agatccagac attttcattt ggaaatatcg
     5041 aacagttctc tgctggaaat cttgttgttc gtctaacaaa tgatatcacg caaatccaaa
     5101 atttagtgat gatttctttg caatcacttt ttcgaattcc attcttattt attggtgcct
     5161 tcattttagc tatgatcacg atgccgcaat tatggtggat catcgtagcg cttatcgtta
     5221 cggtatttct gattacggca ctttcattta caaggatggg caagcacttc atgatcattc
     5281 aaaaactgat tgatcgtgtg aatagcattg caaaagaaaa cttactaggg attcgtgtag
     5341 tgaaatcttt tgtacaagaa gaaaaccaat tgaagtcatt cagcaaagta agtgaggact
     5401 taacaaaaca caacatcata gtcggtacat tattttctgt gatgatcccg tccttcatgc
     5461 tggctgccaa tttggctgtg gttggggcga tcttttttgt gagcgatctt gttaaagatg
     5521 atcctacgtt aattggtgga attgcttcat tcatgaacta cttgatgcag attatgatgg
     5581 caatcattat tggcggaatg atgatgatga tgacttcaag agcagccgta tcgctgaagc
     5641 ggattgcgga aattttagat gctgaaccag atcttactta tttagacgtt ccagaacaag
     5701 agttgacggg ttctgtcact tttgaccatg tttcttttcg ttatcctggt gaggatacag
     5761 atacgcttaa agatatcagc ttttctatca aacctggaga aatggttggt atcgtcgggg
     5821 cgactggtgc cgggaagtca acactagcac aattgatccc tcgtctattt gatccgacag
     5881 aaggcaaagt tgaagtaggt ggtgtagatc ttcgtcaagt aaatgaaaag agtttaagac
     5941 aaacggtttc ctttgttttg caaaaagcaa ttttattctc aggaacgatt gctcaaaact
     6001 tgcgccaagg gaaaaagaat gcaacagaaa aagagatgga acatgcttct tctattgctc
     6061 aagcaaaaga attcatcgaa aagttatctg atcgttacga agcaccaata gaagaacgta
     6121 gcagtaactt ttctggtggt caaaagcaac gtttatccat tactcgtggc gttatcggta
     6181 atccaaagat cttgatccta gatgacagta cgagtgcact ggatgctcgc tctgaaaagc
     6241 tggtccgaga agcactcgat cgagatttga agggaacaac gacgattgtc atcgcacaaa
     6301 aaattgcttc tgttgtcaaa gcagaccgca tcttagtatt ggatgaaggc agattagtcg
     6361 gcgaaggaac ccatgaggaa ttggtagctg gaaaccgtgt ctatcaggaa atctttgaaa
     6421 ctcaaaaagg aacggaggag taagtatgac tgatctaata aaagcaagta aatttttcta
     6481 tcactatttg aagcggtaca aactctcctt tttctttatc tttatcacag tgatcattgc
     6541 tacctatcta caagtaaagg cacctcaata catcggggaa gcttttcaag aattagctaa
     6601 ttacgtagga ggcttgatgc aaggcgtaga cgataaaagc aaatttgttt ctgtaatatg
     6661 gaaactcttg ctgttttatg tactagctag tgcagccaac tttatctata gtattttgtt
     6721 cacacaagta gtagggaagt caacgaaccg aatgcgtatc ggtctattca acaaattgga
     6781 aaaattgacg atccgtttct ttgattctca tcaagacggt gaaattttaa gtcgttttac
     6841 cagtgactta gataatattc aaaatagttt gaaccaagct ttgcttcaag tcatcacgaa
     6901 tgctgtttta cttgtcggga tcttgatcat gatgttccgc caaaatgtag agttagcttg
     6961 ggctacaatt gcttccactc cagtagccgt tttgatagct gtcttgatta tccgcaaagc
     7021 acggaaatat gtggatcttc agcaagatga agtggggaaa ctaaatggtt atatggatga
     7081 aaaaatcagc ggacaacgtg tcatcatcac aaatggctta caagaagaaa cgattgaagg
     7141 attcttagag cataatcaaa gtgttcgcga agcaaccttc aaaggacaag cttattctgg
     7201 tctactattt ccgatgatgc aaggaatgtc actggtcaat acagcgatag ttatcttttt
     7261 cggcggctgg ttagcattaa atggtgattt ggaacgttcg gtggcattag gcttagttgt
     7321 gacattcgtc cagtattcac aacaatatta ccaaccactg atgcagattt catctggata
     7381 cagcatgatc caattagcga ttacaggggc aagacgattg aatgaaatat ttgatgaaaa
     7441 agatgaagtc aaaccagaaa acggtaaagc atttgatggt gttgaaaaag gtcttgcgtt
     7501 gcagcacgta gattttggtt attctccaga aaaactagtc ttgaaagatg tctcgatcaa
     7561 tgtggacaaa ggagaaatgg ttgctcttgt tggtccaaca ggatcgggga aaacgacgat
     7621 catgaatctg ctaaaccgtt tttacgatgt caatgatggt tcagtagtca tcgatggaat
     7681 agatatccga gattatgatc ttgatgcatt acgtagacat gtaggaatcg ttttgcaaga
     7741 atctgtattg ttctccggaa cgatcaaaga caatatcaca ttcggcaaac cggacgcttc
     7801 agatgaagaa gtcatcaatg cagcaaaaca agcgaatatt catgacttca tcatgacgtt
     7861 agaaaaaggt tatgatacag agatatccga agaaaataac ttattcagta caggtcaaaa
     7921 gcagttgatc agtattgctc gaacgatcat cacaaatcca tctttattga ttttggatga
     7981 agcaacaagt aatgtagaca cggtaacaga agcaaggatc caaaaagcaa tggatgaagc
     8041 aatcaaagga agaaccagct ttgtgattgc tcaccggctg aaaacaatct tgaatgcaga
     8101 tagaatcgtt gtcttgcgtg atggggaagt cattgaagaa ggcagtcatc atgagttatt
     8161 agcagaaaat ggattttatg cggaacttta tcataatcaa tttgtatttg aaagttaaat
     8221 taaaagaacc tcgttataac ggggttcttt ttatatattt tatgttgttt gtttttataa
     8281 ggggca
//
LOCUS       DACEUZ010000103         8198 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.103, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000103 DACEUZ010000000
VERSION     DACEUZ010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8198
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.103"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            295..2235
                     /locus_tag="IXG44_002068"
     CDS             295..2235
                     /locus_tag="IXG44_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAQ1658775.1"
                     /translation="MKKIIAITSCPVGIAHTYMAAENLEKAGKAVGAEVKVETHGSIG
                     IENELTARDIEEAAGVIIAADTKIDKSRFGGKPLITVGVQEGIHHADELVRDILAGKA
                     PVYKSTEAIISSENKSESENLGKKIYKSLMNGVSYMVPFVVTGGLLIAISLTLGGTAT
                     PEGIKIPEGTIWATMNSIGSIAMGLMVPILSAFIAQSIADRPGLVPGFVGGMLAANGA
                     LYGSDANAGFLGGIITGFLAGFIALGIKKVRVPKALQSIMPIIVIPILGSLAVGFVFI
                     YVIGEPVALLFSSLTHFLAGMQGGSEIVLAMILGAMIAFDMGGPFNKVAFLFGSGLIA
                     EGNYSIMGPIAVAICIPPLALGLASLILKTKFTKTEREAGKASLAMGLFGITEGAIPF
                     ASTDPLRVIPSIVIGAMTGSVIAMLSGVTDHVAHGGPIVAVLGAVDHVAMFFVAVIAG
                     IAVTVLMLRILKPTLQQTAVAAANGVTVADLSETETSDVYTKTETFDSITDIMSDEAI
                     VLGETATTKDGVIEDLVQRLEKTAAITDKTRFKEVIYDREHESTTGIGMGIAIPHGKS
                     AYVLKPTVAFARSQSGVDWHSLDGKPAHMIFMIAVPENSQGDMHLKILQRLSRKLMDD
                     DFRQALMEAPDKTTVHQLLSEM"
     gene            2322..4244
                     /locus_tag="IXG44_002069"
     CDS             2322..4244
                     /locus_tag="IXG44_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HAQ1658776.1"
                     /translation="MNQRQQSLIYQLSENNNYITTKKLAEHFHVSERTIHSDLNTIET
                     WLRDKHLPLMFERKKGTGILLNGLPHEREQLKRALNEETQTEMDKKQLRQLLIFHLLV
                     AKDGFSLEELSEKLYVGKRTIRKELTELQSFFEYHHLQLVSKTKLGTFLKGDEQEKRQ
                     LLVKTLRNMQKEDPQSPSLKEFFAKDTLKVIQHTLQDVFYENHLEQPNGLASVEIHIY
                     FMLERMKQYQKVKLSKDENEVVEHTQAQKLSSQILAKLATIYPIEFSPDEINYLALRI
                     ANLFTNSQAATRFQKESSALADHLIEQVEQFLGYSLKEDQLLKQNLRSHLSSTYFRLH
                     YGLQISNPLTKNVFSTYTQLFLVLQLILEDYFEKENFYVPQEETAYLTIHFQAAIERH
                     KHQKSRRYQTVLVSEYSKAMATFLEARLNRELPELTIMDLVEYKPELKRQDFPSVDFI
                     LATLPFKHISIPVIEISPMITETDLAYLTKYMLEHVPIKKKKTFDLASFTHPFLIFPQ
                     LERTDPADILNFMGNVLVEHHYVEPEFIDSVLERDSHSSTRVAPFVTIPHGNPLYVKH
                     SMISIATMKEPVLWHGEQIRIVLLLAIKKNDLKHAEFKKIFSVIHYLEKQPEQMAQLM
                     QSNHPLEILTLLSSYE"
     gene            complement(4524..4946)
                     /gene="arsC"
                     /locus_tag="IXG44_002070"
     CDS             complement(4524..4946)
                     /gene="arsC"
                     /locus_tag="IXG44_002070"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (thioredoxin)"
                     /protein_id="HAQ1658777.1"
                     /translation="MKKIYFLCTGNSCRSQIAEGYARKYLSPSMFEIRSAGIETHGLN
                     PRAIKIMAEDGIDISKQTSDLIDRDYFTQADLIITLCGDAKDKCPAVPNNHEHLHWDL
                     KDPAQAVGSEEEILTEFRKVREGIKRKVLDLNQRTSIV"
     gene            5338..6358
                     /locus_tag="IXG44_002071"
                     /pseudo
     CDS             5338..6358
                     /locus_tag="IXG44_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288292.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
     gene            complement(6483..6917)
                     /locus_tag="IXG44_002072"
     CDS             complement(6483..6917)
                     /locus_tag="IXG44_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ1658778.1"
                     /translation="MYSIGQVSEMFGLPVSTLRYYDKEGLFPDIQRVSGIRKFSENEL
                     EALRVIDCLKKSGLEIKDIKQFMEWSKEGSKTFEVRKELFEKQKEVVEKEIAKLERVL
                     AMLNYKSWYYEEAIKAGNEEAVLTMIPDDLPQHVKEAYVHSH"
     gene            7032..8006
                     /locus_tag="IXG44_002073"
     CDS             7032..8006
                     /locus_tag="IXG44_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ1658779.1"
                     /translation="MNKELNITQEWDKVFPKSNQVNHRKVSFPNRFGITLVADLYEPK
                     EVSEKKLPAIAVSGPFGAVKEQSSGLYAQTMAERGFLTIAFDPSFTGESGGVPRYVAS
                     PDINTEDFQAAVDFLSVQKNVNPERIGIIGICGWGGMALNAAAIDTRVKATVVSTMYD
                     MTRVTANGYFDVMDENARYEMREELNAQRIIDFKQGDYARAGGVVDPLPEDAPQFVKD
                     YHAYYKTDRGYHVRSLNSNDGWNRTSGLSLMNAKLLHYIPEIRHAVLVIHGEKAHSRY
                     FGEDAFKELKGDNKELMIIPGASHTDLYDQTDIIPFEKMATFFEENLV"
BASE COUNT         2713 a         1423 c         1664 g         2398 t
ORIGIN      
        1 tcactaactc attagaaaat aagaagctta ttccagaatg aaattaaatg gaataagctt
       61 tttataatta attacggaaa ttgttaatgt agataataat ttagatgtac gtaagcagaa
      121 gtacagaaat gtaagatttt ccaatattat tttattcaac ataataaatc gtatatcaat
      181 ttttttagcg aaatttctat ttattccaag aatttcagtt attacggaat cattttgtgc
      241 ttaataaaaa gaaaccgttt ttatatacta taaatgtaga aaggcggtag gaaaatgaaa
      301 aaaattattg caatcacatc ctgtccagtt ggtattgccc atacatacat ggcagcagaa
      361 aacttagaga aagcggggaa agcagttggc gccgaggtaa aagtcgaaac acatggttcc
      421 atcggtattg aaaatgaatt gactgcaaga gatatcgaag aagcagctgg tgtaatcatc
      481 gcagcagata ctaaaataga taaatcacga ttcggcggta aacctttgat aaccgtaggt
      541 gtgcaagaag gaatccacca tgcagatgag ttggtgagag atattttagc aggaaaagcg
      601 ccagtctata aaagtacgga ggcaattatt tcttccgaaa ataaaagtga atcagaaaat
      661 ttaggtaaaa aaatctataa gagtttgatg aatggcgttt cgtacatggt acctttcgta
      721 gttacaggag ggttattgat cgcgatttct ttaacattag gaggaacagc tacacctgaa
      781 gggatcaaga taccagaagg aacgatttgg gcaacgatga atagtatcgg aagtatcgct
      841 atggggttga tggttccaat tctatctgca tttattgcgc aaagtattgc agatcgtcca
      901 ggacttgttc ctggatttgt tggtgggatg ttagcggcaa acggagcatt atatggtagt
      961 gatgcgaatg cgggattctt aggcgggatc atcactggat ttttagcagg attcattgct
     1021 ttaggaatca agaaagtgcg agtaccgaaa gctttacaat cgatcatgcc aattattgtt
     1081 atcccaattt taggatcatt ggcagttggt tttgtgttta tctatgttat tggagaacca
     1141 gtagctcttc tatttagttc attgactcac tttttagcag ggatgcaagg aggaagtgaa
     1201 attgttctag ctatgatctt aggagcgatg attgcttttg atatgggggg gccgttcaat
     1261 aaagtagcct ttttgttcgg atctggcttg attgcagaag gcaattattc cattatgggg
     1321 ccaattgctg tagctatctg tatccctcca ttagcattgg ggctggcatc attgatttta
     1381 aaaaccaaat ttacgaaaac agaacgagaa gctggtaagg cgtccttagc aatgggactg
     1441 tttgggatta cagaaggcgc tattccgttt gcttcaacag atccactacg ggtgattcca
     1501 agcattgtaa tcggggcaat gacaggaagt gtgatcgcga tgctttcagg agtgacagat
     1561 catgtagctc atggcggtcc gatcgtagcg gttttaggag cagtggatca cgtagccatg
     1621 ttttttgtag cagtgattgc tggtattgct gtaaccgtgc taatgttaag aatccttaaa
     1681 ccgacattac aacaaactgc ggttgctgca gcaaatggtg tgacagtagc agatctatca
     1741 gagactgaaa caagtgatgt ttatacgaaa actgaaactt ttgattctat cacggatatt
     1801 atgtcagatg aagcaatcgt tttaggtgaa acagcaacaa caaaagacgg agtaatcgaa
     1861 gatttagtcc aacgtttaga aaaaacagcg gcgatcacag ataaaaccag atttaaagaa
     1921 gtcatctatg atagagaaca tgaaagtaca acgggaatcg gtatggggat tgctattcca
     1981 catggaaaat cagcttatgt tcttaaacca acagtggcat ttgccagaag tcaatctggg
     2041 gtagattggc attctttaga tggaaaacct gcacatatga tctttatgat tgctgtacct
     2101 gaaaacagcc aaggtgatat gcacttgaaa atactgcaac gtctatcacg taaattgatg
     2161 gatgatgact tccgtcaagc actgatggaa gcgccagata aaacaacagt tcaccaatta
     2221 ttaagcgaaa tgtaacataa gatgaaaaat cgaccgtgat gagacataat agatgtgtta
     2281 tcatgatcga ttttttattt agaagggagt ttctgcacat tatgaatcag cgtcagcaat
     2341 ctttgatcta tcaattaagt gagaacaata attatataac aacaaaaaag ctagcagagc
     2401 atttccatgt gtcagaaaga acgattcata gcgatttgaa taccattgaa acttggttga
     2461 gagacaaaca tctgccttta atgtttgaaa gaaaaaaagg tacaggtatc ttgttgaatg
     2521 gtctgcctca cgaaagggaa caactgaaac gagcgcttaa tgaagagact caaacagaaa
     2581 tggataaaaa acagcttcgt cagttattga ttttccatct gctagtagca aaagacggat
     2641 ttagtcttga agaactttca gaaaaactat atgttggaaa acgtacgatt cgtaaagaat
     2701 tgacagagtt gcaaagcttt tttgagtatc accatttaca gctagtatcc aaaacaaaac
     2761 taggaacttt tcttaaaggc gatgaacaag aaaaaagaca gttgcttgtt aagacattac
     2821 gaaatatgca gaaagaagat ccacaaagtc cgtcgttgaa agaattcttc gctaaagata
     2881 cattaaaagt tatccaacat acattacaag atgtttttta tgaaaaccat ttagaacagc
     2941 cgaatggact ggctagtgtg gagattcaca tctactttat gctcgagcga atgaaacagt
     3001 accagaaagt gaaattaagt aaagacgaaa acgaagtcgt ggaacacacc caagcacaaa
     3061 agttaagtag tcaaatatta gcaaaactag ccaccatcta tccaattgaa ttttctcctg
     3121 atgaaatcaa ttatctggct ttgcgtatcg ccaatctttt tacaaattca caagcagcca
     3181 cacgttttca aaaggaatct agcgcacttg ctgatcattt gatcgaacaa gtggaacagt
     3241 ttctaggcta ttcattaaaa gaagatcagc tactaaaaca aaatttaagg tctcatttat
     3301 cttcaactta ttttcgactc cattatggtt tgcagatttc taatccattg acgaaaaatg
     3361 tgtttagtac gtacacacaa cttttcttag ttttacagtt aattttagaa gattattttg
     3421 aaaaggaaaa tttttatgtt ccacaagagg agacagctta tttaacgatc catttccaag
     3481 cagcgatcga gcgacacaaa catcagaaaa gtagaagata tcaaacggtt ttagttagtg
     3541 aatattcaaa agctatggct acttttctcg aagcacggct gaacagagaa cttccagaat
     3601 taacgattat ggatctagtt gaatataagc cagaattaaa gagacaagat tttccctctg
     3661 ttgattttat cttggctacg ttaccattta aacatataag cattccagta atcgagatct
     3721 caccaatgat cacagaaact gatctagcct atttgacaaa atatatgctg gagcatgtgc
     3781 ctatcaagaa gaaaaaaaca tttgatctag caagctttac tcatccattt ctgatctttc
     3841 ctcaacttga acggacagac ccagccgata ttttaaactt tatgggaaac gtcttagtgg
     3901 aacatcatta tgtggagccg gaattcatcg attctgtctt ggaaagagac agtcactcca
     3961 gtactagagt agcaccattt gtaacaatcc ctcacggtaa tccgctgtat gtgaagcatt
     4021 cgatgatttc tattgctaca atgaaagaac cagtcctatg gcacggggaa cagatacgta
     4081 tcgtactgct attagcaata aagaaaaatg atttgaaaca tgcagagttc aaaaaaatat
     4141 tttctgttat tcactatcta gaaaagcagc cagaacaaat ggctcagttg atgcaatcca
     4201 atcatccgct agaaatctta acgttgttat ctagttatga ataagtagcg attaaaaaac
     4261 tgcctttata tttataataa ttctatctat ttatttggaa gtagaaaact aaatacaaaa
     4321 aaagaggatg tggccaaagt tgtggcacat ccttttattt tgagtttatt ataatttttt
     4381 atagttgctt acgatgtctt gactactggt aaacggtgtt caagagacaa tttgtcggca
     4441 ataaggcaca attttgtaaa atatggaaag ctattttttt aacattgttt cttagtgctt
     4501 gaaaaatcat tttgaatact tctttatacg atcgatgtac gttgatttaa gtctaaaact
     4561 tttcttttga ttccttctct gacttttctg aattccgtta atatttcttc ttcgctcccg
     4621 acagcttgtg caggatcttt taggtcccag tgaaggtgtt cgtgattgtt tggaacggca
     4681 gggcacttgt ctttggcatc accacaaagt gtaatgatca agtctgcctg agtaaagtaa
     4741 tctctatcaa tcaaatcaga cgtctgtttg gaaatatcga tgccgtcttc tgccatgatt
     4801 tttatagctc ttggattcaa gccatgtgtc tcgatccccg cacttcttat ctcaaacatt
     4861 gaaggagaca agtatttacg cgcgtagcct tctgctattt gactgcggca tgaattgccc
     4921 gtacataaga aatagatttt tttcatttga tttcctccgt tatggattag taaatgtcac
     4981 tgtttatcta aagtaaactc tatgtgatgg agcgaaaaaa caatgggttt cttagtttat
     5041 atatttcttt ttgttcagcg tataaatcga tgtgtacaaa gaactaatct tgtctttttt
     5101 accataaata catgttagaa acaatcattt tgagtcaaaa atcaacgatg taacagactg
     5161 ttaaaagaga ttaagacacg gcagatgatg aagaaagtag gggtgtagta agccgataca
     5221 tttcttgatt gtgaggcgag aacaattatt cgtcttttaa gaaaaaaagt agtagaatca
     5281 gttcatatta aaaataaaaa accttccatt tttgtaggtt taaaggaggt cattaccttg
     5341 aaaaaaatcg gctttttaag ctttggacat tggatggagc aaggatcgct tgtgaaatca
     5401 gctcaagatg cttatttgca atcgattgat ttagcggtgg aagcagaaaa aatcggagtg
     5461 gacggagcgt ttttccgcgt acatcatttt gcccctcaga ttgggtcgcc gtttcctttg
     5521 ctagcggcaa tcggcgcaaa gacaagtaag attgaaatcg gtacaggact tattgatatg
     5581 cgatatgaaa acccttatta tatggcagaa aatgccggat tgacagatat catcacgcaa
     5641 ggaagagtgc aactaggagt ggggcgtggt tcacctgaac aagttctaga tggctggaaa
     5701 tactttggtt atgattactg aagaagaaat caaagaggta acacgggaac gcacccttga
     5761 attcttggaa ttacttgaag gaaaaccatt tgaagaacca aatcctcaac caatgttccc
     5821 acaaggatca ggaaaattac ggatagaacc ttattcagaa ggattacgac aaagaatctg
     5881 gtggggagct ggttcgcagc aaacggcaat ctgggcagca caacatggaa tgaatctgca
     5941 atcatctaca ttagtcaatt acgaatccca cgagccattc catatccaac aagcaaacca
     6001 attacgtgcg tataaagaag cgtggaagca agcaggtcat gattttgaac caagaacatt
     6061 agttactcgc tcgattcagc caatcaccac tgatttggat cgacgcttat ttggacaaga
     6121 cggtagtccg caacaagatg ctgtcggcta tcttgcttat caccgaaacc caacgatttt
     6181 tggaaaaact tttgctggtg aacctgatca attaatcaag gagctagctg aagatgaagc
     6241 aatcaaagaa gcagacacag tacttgtcac gattcctaat actttggggg tagaatataa
     6301 catgcacttg cttgaaacaa tcgtaaaaga tatcgctcca gaattaggat ggcggtaaaa
     6361 aggatatttt tgtaagtgca ggatacttga gtgaaaaaat aaacacttct aacaatcagt
     6421 tagaggtgtt tattcatgtt ttcatgaagt tcatagagaa ggagagtgag ttatccgttt
     6481 gttcaatgag aatgtacata agcttctttt acatgttggg gtaaatcatc aggaatcatt
     6541 gtcaatacgg cttcttcatt gccggctttt attgcttctt catagtacca gcttttgtag
     6601 tttagcatag cgagtactct ttctaatttt gctatttctt tttctactac ttctttttgt
     6661 ttttcaaata gttctttacg aacctcaaat gtcttactgc cttctttgct ccattccatg
     6721 aattgcttaa tgtcttttat ttctaaacca gattttttca aacagtcaat gactcttaaa
     6781 gcctctaact cattctctga aaattttcta ataccagaaa cacgctggat atcaggaaat
     6841 aatccctctt tgtcgtaata acgtaaagta gaaacaggca atccaaacat ttcagacact
     6901 tgtccaatgg aatacataaa aaaatcctcc taaaataaaa tttttacctt gacctaaagt
     6961 caagtttaag ttttatcata ataatacatt caaaaacaaa tgtcaatttt gatcaaagga
     7021 gagtctagaa tatgaataaa gaattaaaca tcacacaaga atgggacaaa gtatttccaa
     7081 aaagcaatca agtcaatcat cgaaaggtga gcttcccgaa tcgttttggt atcacattag
     7141 tcgctgattt atatgaacca aaagaagtaa gtgaaaagaa attgccggca atcgctgtga
     7201 gcgggccatt tggtgcagtg aaggaacaat cgtctggtct atatgcgcag acgatggctg
     7261 aaagaggctt tttaacgatt gcgtttgatc catcgtttac aggagaaagc ggaggagtac
     7321 ctcgatatgt tgcttcgcct gatatcaata cggaagattt ccaagcggca gtagactttt
     7381 tatctgtaca aaaaaatgta aaccctgaga gaatcggaat cattggtatt tgtggatggg
     7441 gaggtatggc attgaatgca gcagctatcg atacgcgggt caaagcaaca gttgtttcca
     7501 caatgtatga catgactcga gtaacagcaa atggctactt cgatgttatg gatgaaaatg
     7561 cccgatatga aatgcgtgaa gaattaaatg cacaacgcat cattgatttc aaacaagggg
     7621 actacgcacg tgctggaggt gtggttgatc cgttgcctga agatgctcct caatttgtca
     7681 aagactatca tgcgtattat aaaacagacc gcggatatca tgtccgttct ttaaactcca
     7741 atgatggttg gaataggaca tcagggttgt cacttatgaa tgcaaaacta ttgcattata
     7801 ttccagaaat ccgtcatgca gtcttagtca ttcacgggga aaaagctcat tcacgttact
     7861 ttggggagga cgctttcaaa gaactaaaag gtgacaacaa ggaactcatg atcattccag
     7921 gagcatccca tacagatcta tacgaccaga cagacatcat tccatttgaa aaaatggcaa
     7981 cattctttga agagaacctt gtgtaaccaa aaggatttaa tctaaaagag attatgaaaa
     8041 aactgccctg catcaagtaa taaggacagc caaaaaagct cctcattttt gacttggttg
     8101 gactcattag cgtagatgca agcttttgaa caccgtttat accaagaggt cgtgagcctg
     8161 ccgtttaact ctgagtcaca aggagcaatt tcaaaaaa
//
LOCUS       DACEUZ010000104         8097 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.104, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000104 DACEUZ010000000
VERSION     DACEUZ010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8097
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.104"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(310..807)
                     /locus_tag="IXG44_002074"
     CDS             complement(310..807)
                     /locus_tag="IXG44_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ1658780.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(847..1656)
                     /locus_tag="IXG44_002075"
     CDS             complement(847..1656)
                     /locus_tag="IXG44_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ1658781.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            1990..2847
                     /locus_tag="IXG44_002076"
     CDS             1990..2847
                     /locus_tag="IXG44_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAQ1658782.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            2840..3742
                     /locus_tag="IXG44_002077"
     CDS             2840..3742
                     /locus_tag="IXG44_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAQ1658783.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            3775..4125
                     /locus_tag="IXG44_002078"
     CDS             3775..4125
                     /locus_tag="IXG44_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAQ1658784.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            4159..5097
                     /locus_tag="IXG44_002079"
     CDS             4159..5097
                     /locus_tag="IXG44_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAQ1658785.1"
                     /translation="MRYYFVDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPD
                     DIFVGTEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLI
                     TCIDRKTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAAR
                     KTTENEELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQ
                     RQTEKMIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTG
                     GLPGKSSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            5175..6068
                     /locus_tag="IXG44_002080"
     CDS             5175..6068
                     /locus_tag="IXG44_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ1658786.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            6106..6669
                     /gene="amaP"
                     /locus_tag="IXG44_002081"
     CDS             6106..6669
                     /gene="amaP"
                     /locus_tag="IXG44_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAQ1658787.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            6683..6871
                     /locus_tag="IXG44_002082"
     CDS             6683..6871
                     /locus_tag="IXG44_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAQ1658788.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            6884..7786
                     /locus_tag="IXG44_002083"
     CDS             6884..7786
                     /locus_tag="IXG44_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ1658789.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            7818..8060
                     /locus_tag="IXG44_002084"
     CDS             7818..8060
                     /locus_tag="IXG44_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ1658790.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
BASE COUNT         2805 a         1414 c         1586 g         2292 t
ORIGIN      
        1 ttggctgttc ttattactgc agatgcaagc ttttgaatac cgtttaagga agaggttgtg
       61 aaatcagcgt tttgacctga gtattagctt aacaaacgga aaaatagttc ctcatatttt
      121 gccgtttgtt aagctaatac cgagggtcag ctgattgaac accatttaag aaagaggtcg
      181 tgagcctgcc gtttcactct gtgtcataaa aaagccaatc tactttgata gaaaaagcac
      241 gtaggactaa aacagttctg cgtgcttttt ttattgtgtg ttaaatatcg taaatgaaga
      301 tagatatttt tatgattcga cgatagagac gttgaaatct ttcaacagct ctaaagcgga
      361 ttctccgtgt tttctactgg agactaacat tggatcaaca aaaaagtctg cttcaggaaa
      421 agcactctgc aggcctatca gattagagag tacacacaag tttgtttcaa tcccgactac
      481 ttcgatctct ttttgttctt ctttgctttc aaataaagtt tcctggattt cagctaaagt
      541 ttcaggtgga atcgtaaagt aatacttttt aatcactcgt tcattttctt gtggagaaag
      601 aagagggatc aattgcaaat cttctcgttc ttcttctttg tccttcagtt caactggtat
      661 atcccgtgtg aagagaatat attcattatt ttggcgtgct ttagctaatc tttgagcaat
      721 acgttctgcc aattgatctg actctggaat ataaaattcg ctgtttggat caagaatatg
      781 attttgcata tcaataataa ctaacatttt ctttcctcct acattcatat cgtcatttta
      841 ttctttttaa tagaagtttt ttaaaatata agcaacacca gcttcttcat tcgtcagtgt
      901 tacttggttt gcaatttctt ttacttgtcg ctgtgcattc cccatcgcaa tactcaaccc
      961 gctgtaattc aacatagcaa tgtcgttttc ttgatcgcca aaagccatga tgtcactcga
     1021 ttcgattcca aggtaatccg ccagaaaaac aagtgaatga gctttagtga tacctgtagg
     1081 cgtgatttct aagaaatgcg gatcagagaa tactgattgg ataccatctg cttgcagatc
     1141 tctttgtagc ttctgtagtt tttcttcact cttatctgtt aatgctaatt taagatagct
     1201 aagagaagga tcttccaaca tatttaaaat ttgatcaaaa gaatggttct gtaaattgaa
     1261 atgttcataa taagggtcat ctttgactag atttgattca tcgaatgtca gctgatcatc
     1321 ttgggtttct cggctagcaa tgatggatat tttcgcttct tgagcataga agaaggcttt
     1381 tctcagtgta tcgctatcta tttttgaacg tctgagaact ttcgccattt ttccatcctt
     1441 catttcagca atcagtgaac cattggaaga aatcaaatag ccaccgattc ctaattcgga
     1501 aaaaatatct ttagcagaaa tgctgttcct tccggtagcg acggttacga ttccgccatt
     1561 ttcatcaaat ttccgaagtg cttggatatt ttcttctggt atctttccat ttcgatcaag
     1621 caaggtaccg tctaaatcaa tcgctgctag tttcatttta tcttcctact ttctttttac
     1681 atatctattt tcctgttacc ttaagattaa atgagaattt aaaaaaatga aactaacttg
     1741 ctttgtcatt atgaattttt tcaaaaaaca ttcttaagaa aaaggtggct atatatagta
     1801 gtttttggtc aatacctttg aataaaaagc catgaaaaac gaatagttag cgcttaccga
     1861 tgcgttttgt gataaaaaaa ttttaaaata ttcaatgaaa acaaagcata tattttgctt
     1921 taaaaccaaa tgaggaatat tctagatgta gaacaggtta gaaaaagatt agaaaaagga
     1981 gtgattttga tggagaagag atacggcgta gttcctgtgg taactgcttc agacgaaaat
     2041 tatgctcctt acttgagcgt gatgatcgca actgcattag aaaattgcaa taaaacaaga
     2101 cgcataaaat tttatgtgat tgacgatggt ctatcagaat atagtaaaga aggattggaa
     2161 gaaacggtaa ataaatattc aagtaatgca agtatccaat tcctgacagt ggaaaaagat
     2221 atctacgaag acttcctagt cagtgaccat atcacaacta ccgcttactt aagaatttct
     2281 ttaccaaact tattagctaa agaggattat aagaaagtcc tttacttaga ttcagatgtg
     2341 ttagtactag acgatatcgt aaaactatat gatgagccgt taaatggaaa aacaatcggc
     2401 gctattatcg atccaggcca agtgaaagct ttggaaagat taggaatcga ttccgatgac
     2461 ttgtacttca attcaggagt gatggtgatt gacattgatc aatggaataa aaaagaaatt
     2521 acagaaaaaa ctatccatta cctcagtgaa aatggcgatc gaatcatcta tcacgaccaa
     2581 gatgctttga atgctgtact ttatgaagat tgggaacaac ttcatccaaa atggaacatg
     2641 cagacttcgc tgatttttga acgtcatcca gcaccaaatg aaaaatatga acgtcagtat
     2701 aaagaaggaa atgaaaaacc ttcgatcgtt cactttacag gacatgataa accttggaat
     2761 acgttgaaag atcatccgta taccaattta tacttaaaaa aattagcaca cagtactcta
     2821 acgaaagtag gcgagatcaa tgaataaaaa agaaatagca gtagtcgcaa gttgtaacac
     2881 aaaattcgta ccacatttag ctgcattatt tgtctcagta ttagataatt gcaacccgtc
     2941 taaatttgtt cggttctatg tgattgacga tgatatcgat tttgaaagta aacagctttt
     3001 acgcttctca gtgaaaaacg cacgaatgaa ctcagatgtg gaattcttga aaatcaataa
     3061 agaattcttt acaaatgtgg taatcagcga tcgtattcca gaaacagctt attatcgtat
     3121 tgcgattccg gaattgttcc gcggcacaga agttgaacgt atcttatata tggattgcga
     3181 catgatcgct ttacaagata tctctaaatt atggcgtctt gacttcggtg attcaatcgt
     3241 agcagctgtt gaagacgctg ggttccatca acgactggaa aaaatgaaaa tttcagctaa
     3301 atcaatgcgt tatttcaact caggtttgat gctgatcaat gtgaaaaaat ggttagatga
     3361 aaatattacg caaaaagtat tagatttcat cgaacataat ccagaaaaat tacgattcca
     3421 tgaccaagat gcattaaatg ctatcttaca tgaccgttgg ttaccacttc atccgcgttg
     3481 gaatgcacaa ggctatatca tggcaaaagc aaaaaaacat ccaacagcag caggtgaacg
     3541 tgaatacgaa gaaactcgca acaatcctta cattatccat ttttcaggac atgtgaaacc
     3601 gtggagcaag gattttgaag ggccaacgaa gaaatattac gaaaaatatg cagggatgac
     3661 ggctttccgt tgtgtagcta aattccctaa atatccaaaa tatgcaaaag ttcaaccaaa
     3721 aacagatgta gttgttaaat aatagaagac aacaactgaa gagggggagt aggaatggaa
     3781 attacaatgt ctaaaaatgc agtagagacg ttaattgaaa aggttgggga aaatacaaaa
     3841 atcgcactcg cgcttatcaa tgattccgat ccatttctaa gggacaaagg tgcttttgca
     3901 aaaggcagct tttttcaaat cattcctttt gtttcggaat ttggagaata tgcgactaaa
     3961 atcgagcatc cattattgga catctataca tctaaattag agcaaaatta tttcggtaaa
     4021 cgattgaaca tggacttcaa caaacagctt gacagctttt cattagaaaa tgaaatcgca
     4081 gtactcgatt ataatatcaa attaaagaac tgcttcttca gctaatagtt tcgagaaagt
     4141 ccaaaaagga ggtatacatt gagatactac tttgtggatg aagaaaagaa tgtaatagaa
     4201 acagatgaaa aaaaatataa ttggctcgta cttgatagtg caaagcccga agagattgag
     4261 caagcaatcg aagaattcga gcttcctgat gatattttcg taggaaccga atatccggaa
     4321 gaagtttctc ggatggagcg cctttatgag acgaaactag agcatccatt ttctttggta
     4381 gtgattaatc tagatcagtc agataatcgt atcgaaaaaa aattaacgcc aatatctttt
     4441 gtactttccg ataatctctt gattacttgt atcgatagaa aaaccgaatt tgtggatcgg
     4501 ctgctgacaa agcatggaaa tcatttaaac tcgtttgaaa agatcatcac gtatacatta
     4561 ttagatattt acacccatta cgtgaaagaa ctaagagaga tgaaaaagcg aattgacgct
     4621 ttggataaag cagctcgtaa aacaacggaa aatgaagaac tgtataaaca ggcagatctt
     4681 gaaagagaca tcgtatatat cgatcatacc ttaagagatc aacgggaaac aatggatcaa
     4741 ttatggaatt ctgaaacttt catcaaacgt ttagacgatg aacagctact ctatgatgtc
     4801 catttgagac agcgacaaac agaaaaaatg atcgagatct atcgagattt attagagagt
     4861 attggtgatc tattcaacgg gatgatggac aataatctaa atcatatcat gaaatatcta
     4921 gattcagctg cattaatcat ttccgttccg gcgttgttcg cagggatttg gggaatgaat
     4981 acaggaggat taccgggtaa aagttctgct ctaggcttta tactcgtagt ctttggctct
     5041 ttgatagcag cagtagtttt aggttaccat ttataccgga aagattatac aaattaaaga
     5101 atctatttaa aacaggtatc aaacaaagaa atttgttttg gcaatacaat ttaaaaacta
     5161 ggaggaatac agcaatgaca gaaccaaaat taatcgaccc gcgaaacttg taccatacgg
     5221 agaaatttcc taaacaagat caggatacac cagctttaca gcagaatatg attcctgttc
     5281 ctgattgtgg agaagaatcc tatgaaggca ataatcaact agaaaaccgc cgtgtactaa
     5341 tcactggtgg agattcaggg atcggtcgtg cagcagcgat tgcatttgct agagaagggg
     5401 cagatatcgc tcttcaattc ttcccaggag aagaaaaaga tgcagaggaa gtagcaaaat
     5461 acgtgaaaga tgcaggacgt aagattgtct tactaccttt tgatcttcgt gacgaacaag
     5521 cacctaaaga aatcatagat aaagccgtga gtgaacttgg cgggctagat acgatggtac
     5581 taaacgcagg acagcaaatt tcttgtcagt ctattgctga gttaccactt gagcaagtca
     5641 aagacacatt tatgataaac atcatcgcta tgtttgcttt agtgaaagaa gctgttccgc
     5701 atatcccagc aggaggagca atcgtaacaa caacttctgt tcaagcattc aatccaagcg
     5761 aacatttatt agattacgca gcaacaaaag cttcaatcgc taactttacg atcggtttag
     5821 caaaacagct tgcacctaaa ggaatccgtg taaatggtgt agcacctggt ccaatctgga
     5881 caccattaca actagacgaa ggacaacctt ctgattcgat ccctgaattt ggtcagcaaa
     5941 gtttactaga acgtgcaggt cagccaggag aattagcacc agtttatgtt ttcttagcat
     6001 cgaataaagc aagttatgtt acagcccaaa tttacggagt aactggtgga gaagcgatca
     6061 acttataaga agttgatcgt ttcaccccta tgaggaggtg cgaaaatgaa taaaggtgta
     6121 aaaacgatcg gagtgatcgg aacattactt ttgctatctg tgcttttgtt tgcgatacta
     6181 ttaagtgcaa cctatccatt gccttttcag ctagagaagt ttcgattctt ttccatcact
     6241 aattcatacg tgcaacaata tgtattttgg cttgccgttg catttgctat tttgacaatt
     6301 atcacgttac ttgttttcct gttttatcca aaaacaatcg gaaactttct actgaaacat
     6361 ggtgatgggg agttgacgtt ggataaaaaa gcaattgaag gaatggttcg ttctcacttg
     6421 catgaagatg aattcatcca ttcaccaaaa gtaaatgtca aagcaacgaa aaacaagatc
     6481 gccgtcaatg taaaagggga gttgaaacga acttcagcac tcattggtaa aacaggaaca
     6541 ttgatgaaag aaatcgaaaa tgaagtacaa cacatattgg gaacaaaaga gccagtgaaa
     6601 gtatcggtga aatacacagg ctttgaagaa ccagctaaac gaagcaatga ccgcaaacga
     6661 gtagaatagg gggggaccaa aaatgaaaga actattttcg acatacaagc tccctatcat
     6721 ctttggggta cttggattga tattagccgt tttatggatc accgtgggct ttttcaagac
     6781 cttattgatc ttgatcatgg cagcgatcgg cgtcgctatc ggtttttatc tgaaacaaac
     6841 gaaaatctta gatcactatt ttaaatctta ggaggaaaca tccatgaata acaataacaa
     6901 aaataataac aataaaatga acaaccaaaa accagtgtct gcacatccag acccaacgac
     6961 accaaaccaa tcaaatggtg tgaacgatga aaagaacaaa acagcaccag tatctgcaca
     7021 tccagatcca gtagtcccta acgttcaaca aggagtagaa gagaagaaac atgatgcaga
     7081 aaagcatgca cctcatcatc caggaactcc agaatctaaa gttccagaac aacatgatgc
     7141 aatcgatcct tctaaacctg tggaagagca aaaacatcct tctgaccatg cgaaagcaca
     7201 agaaaaactt agttctgacc acacaaacgg acaaggaaaa cttggttctg atcatacaaa
     7261 taaccaagga aagcatgatt ctgaacagga agattcaatg cgcggtgagc tgaattatga
     7321 agataaagtt gtgcaaaaaa tcatcggaat cgcaattgaa caagtagacg gtttattatc
     7381 tgcaaacggt ggtttcttct ctaatgtggc aggaaaactt gtgaatacag ataatgtaac
     7441 ggctgggatc gaaacagaag tcggtaaaaa acaagtagca gttgatatgg atgtcattgt
     7501 ggaatatggc aaagatatcg aaaaaatctt tgaagaaatg caagaagtaa tcggcaaaga
     7561 agtaaaaaat atgacacatt tagaagtgat cgaagtcaac gctaacgtag tggacatcaa
     7621 aacaaaagaa gaatttgaaa aagatcaaga aacggtgcaa gacaaagtaa ctgacgcagc
     7681 gaaaaaaacc ggtgaatttg cttcgaatca aacagataaa gtaaaatcag cagctggaag
     7741 cggcgcacaa aaagtaaaag aaaatacaga accacgggtt cagtaataag gaagtaaaac
     7801 attaggagga acgaaacatg ggatttattt ggtcacttat cgtcggagga attatcggag
     7861 caattgcagg agcaatcacg aataaaggtt cttcgatggg gattattgca aatgtcgtag
     7921 caggtttagt tggatcagca cttggtcaag cattattagg ttcttgggga ccaagtattg
     7981 caggaatggc aatcgtgcca tcattgattg gtgccatcat tttagtacta atcgtatcat
     8041 tctttgctcg aaaactgtaa aaagactttt aaaaagaggt cgtgaaaaag ctgttct
//
LOCUS       DACEUZ010000105         7761 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.105, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000105 DACEUZ010000000
VERSION     DACEUZ010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7761
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.105"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            147..1094
                     /locus_tag="IXG44_002085"
     CDS             147..1094
                     /locus_tag="IXG44_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1658791.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            1106..2026
                     /locus_tag="IXG44_002086"
     CDS             1106..2026
                     /locus_tag="IXG44_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1658792.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            2067..3524
                     /locus_tag="IXG44_002087"
     CDS             2067..3524
                     /locus_tag="IXG44_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1658793.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            3869..4483
                     /locus_tag="IXG44_002088"
     CDS             3869..4483
                     /locus_tag="IXG44_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAQ1658794.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYSSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            4476..6200
                     /locus_tag="IXG44_002089"
     CDS             4476..6200
                     /locus_tag="IXG44_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ1658795.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            6197..7648
                     /locus_tag="IXG44_002090"
     CDS             6197..7648
                     /locus_tag="IXG44_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1658796.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
BASE COUNT         2618 a         1292 c         1543 g         2308 t
ORIGIN      
        1 ccgttttttt ctctaatact caggccaaaa cgctgatttc acaacctctc ctatactttg
       61 tttcattttc cctatacttt gtttatttat tctcattatt gaaagcgcta taattacgtt
      121 gcgaatgaat cgaaaggggt ttttagatga aaaaacgaaa ggggttcaat cattttttta
      181 tgaatctatg gcgttataaa gcacttgtcc tgatggctgt accgggtatg ctttggatga
      241 tttttttctt ttacatacct gtaatggcga atgtcgtagc attcaaaaat tttcatattt
      301 cagcagatgg gttcttggct agcttgaggg aaagtccttg ggtgggttgg gaaaatttca
      361 aatttttatt ttcttcggat caagcatttc tcatcactaa aaatacaatt ttatataatg
      421 tagcatttat ccttttgaat ctgctgatct cagtcgtatt tgcaatcatt atgagcgagt
      481 tgagaaataa acgactcgtt aaagtttatc aaacgatgtc cttactacct tattttctat
      541 cttgggtcat tatcagttac tttgtttacg cttttttaag tcctgacaaa ggaatattca
      601 atcagtggat cactggacat ggaggagaag ctgtcaattg gtataatgaa ccgaaatatt
      661 ggccgtttat cttggtgttc attggtacat ggaaagggat tggttataac agcattattt
      721 actttgcatc tgttatggga atcgatccga cttattatga agctgctatg gttgatggag
      781 ctagcaaatg gcagcagatc aaacatgtga cgataccaca acttattcca ctaatgacga
      841 ttttaacgat tttagccgta ggaaacattt tccgagctga ttttggcctg ttttataaca
      901 ttcctaggaa ttccggtgca ttatatgatg tgactcaagt tttggatacg tatatctaca
      961 atggcctgac atccacagga gattttggta tgacggctgc tgcaggactt taccaatcag
     1021 ttgtcggctt tgtcttgttg atgatcacaa atacgatcgc ccgacgtttt gatagcgatt
     1081 ctgcattatt ctaaggagga aaaaaatgaa aaaaaagaaa gtggagagag tccaagtccg
     1141 ctctttcaat aagacggcta attttttctc taatataatg attgcactct ttgctatttc
     1201 ctgtattttg ccatttatat ttgttatcgc catctctttt accgatgagt ccatattgtc
     1261 gactcacggg tacagttttt ggccagaaaa gttcagtata tttggttata cgtacttgtt
     1321 cgaccagatg caggataaaa ttttccaagc gcttttcgtc actgtacttg ttactgtttt
     1381 aggtacactg atcaatagca cggctacttc tttatatgct tatgcgattt cacggtcaaa
     1441 ctttccattc cgtcgttttt ttaccgtttt ttgtttgatt acgatgttgt tctcaccagg
     1501 gatggtggcc aattatcttg ttatgacaaa tcttcttcag ttaaaagata cgatttgggc
     1561 attgatcttg ccaatggctg tcagtccctt caatattatt gtgatgcgca cattctttaa
     1621 acgttcggtc tctgattcga ttattgaatc ggcacggatc gatggagcta gcgaactgcg
     1681 aatatttgtt cagatcgttt taccgttagc tgttccaggt attgcgacaa tcagtttatt
     1741 tgccgcattg ggttactgga atgactggtt caatgcttta ctatacatcc aagatgataa
     1801 gctagttcca ctacagtatc ttttgatgaa aatccaaagt aatattcaat atctgacaca
     1861 aaattcagga gcaggaagcc aattgacagg cggattagcc tcagttccag gagaatctgc
     1921 cagaatggcg atcgtagtca tctcgacgtt gcctattgcg atcagctatc catttttcca
     1981 gaagcatttt gtcaaaggtc taacaatcgg cggagtaaaa gaataaagaa acaaaaatat
     2041 aaatcataaa attaggagga agaaaaatga agttatggaa gaaactagcc tttacaggtg
     2101 tttcagcgtt gttgttggga acattagcag catgcggagg ttctggttct aaagaccagg
     2161 cagaagctag caattcggac actttgcaga tgtatcaaat cggagataaa ccagataatt
     2221 tcgatcaatt gatggaagta gcaaacaaac gaatcgaaaa agagatcggt gtaaaagtca
     2281 atatcaatta cattggctgg ggcgattacg aaaagaaaat gaacgtcatc atttcttccg
     2341 gggaaaatta tgatatcgcc tttgcgaaca actatgtttc caatgcccaa aaaggagcct
     2401 ttgctgattt gactgaatta gcgcctaagt acgctgaaaa agcttataat gatttagatg
     2461 aagcatatat taaaggaaat ctggtcaatg gaaaattata tgctttccca gtcaatggaa
     2521 atgtctttgc tcaacaagta cttactttta acaaagcttt actagataaa tacgatcttt
     2581 caattgatgg aatccaatct tacgctgatg cagaaaaagt cttgaaggaa ttccatgaaa
     2641 aagaaccaaa cacggcagct tttgccattg gacaaggctt caaagtccaa gggaactttg
     2701 attatccttt agggaatacg ttaccatttg ctattgatct ggatggagat gattcgaaaa
     2761 tcatcaacca atacgataat gaaacgtata tcggactgtt acgtacaatg catagctggt
     2821 acaaacaagg actcattcca agtgacgctg caacgagcaa taaggattat ccactagaag
     2881 gaaatacttg gctaatgcgt ggagaaacac aaggaccgta tgattatggc gatacaattt
     2941 tgacaaatgc agcaaaacaa gaacttgtct caaaagcaat tactgttcca ctaaaatcta
     3001 cagctcaagc acaaatggca aattttgtcg tatcaaatac ttcaaaaaat aaagaaaaat
     3061 cagtagaact tctagggctg cttaacagtg atccagaatt attgaatggg cttgtttggg
     3121 ggatcgaggg agaagcatgg gaaaaagtcg atggttcaga tgataaaatc aaattgctag
     3181 atggttatca gccaaataca catatgtctg cttggaatac aggaaacaac aagattctat
     3241 atacacaaga gtctattacg gatgacatga ttgccaaacg tgatcaatcg attgctgatg
     3301 cagaaacttc accgatttta ggattcagct tcaacactga cagtgtcaaa acagaattaa
     3361 gtaatatttc taatgtcatg aatcaatatt tagatggatt gaatacaggg actgttgatc
     3421 cagatgaaac attaccgaaa ttgaaagacg ctttggacaa ggctggctat gataaggtat
     3481 tgaaagaaat gcaaaaacaa tatgatgagt tccgacaaga ataataatga ataaagacaa
     3541 aggggttgtg acaaaagacg tgtcacaacc tcttgttccg aataaacggt gttcaaaagc
     3601 cagtttctcg gtattaagct aaatatatgt aaaaatgtgg aaagccatat tcacaaaatt
     3661 tttcttacta ctcgaaacta agcggctatt tcacaaactc ttggtaaacg gtgttcaatc
     3721 agctaaccct cggtattcgc tcacaaaacg acaaaatatg aggaactatt tttccgtttg
     3781 tttctctaat aatcaggtca aggtgctgat tgcccaacct ttttttgtat aatgagaaag
     3841 tataaactta atgtttggag gagaagatat gttagggaag gttttggaag agttatttat
     3901 ccggatctgg gtcattataa aactaacctt gtatttctgg atatatacat ttgccggcgg
     3961 gatcatcttt ggattaggtg cggcatggaa aacagtcaat gaactatttt atttgtacgg
     4021 attcgagtat aaagaaatca caataaaaag aggctggaat atatataaaa gaaatttttt
     4081 aagaggcaat ctgctattta gtttattttt atctggtact gctttattga gttacaattt
     4141 atattcatct gttcaaatca aagggctcat ttttttagct attgatttta ttttactttt
     4201 tgccttgctc tgcctatttg ctacttatca atacagtatg attttagata gtgaatatac
     4261 gatctctgtt cctaatctgc taaagctaag ttttatttcg gttttttcca gcttttcgtc
     4321 ttttctaaaa ataattattg gtagcggtat aattttaggc gtgacttggc aattcaaagg
     4381 attgatcctg tttggtgtca ttggtctttt gacggtttgg aatggtacga tgacaacaca
     4441 ttggcgtgaa gaattagata aacagttgga aagttatgaa taagtttcgg attctatttt
     4501 caaaaggaac cttactaaat cgtctgttgc ggaaatacgc ggttatcatg atcggtgtaa
     4561 cgatgactgc taccgttatt tttagtatcc atacttggga acaaaatcaa aaacaggcag
     4621 aaaacatgac aagtgatgcc gttcaaagca caagcagaat gctgaatgat aaaacaacct
     4681 tgagtaggat catcaaaaac cagcttgtag ggaattccga aaagatcgaa aatgtgacga
     4741 cctacttaac aaaacccata gatcagtatc tgatgtatgt atacgaacag caaaacagta
     4801 ccgatgaatt agtttctttc ccaaatcaaa tcaaagatct ctacgcaaat tatgaagaac
     4861 taagtgcgat atatatcgtg ttgaatcaac taccggagta ttatgaatct tctagagaaa
     4921 acaaaggtgg ggaaataaaa aaggggacac caaaattact agacgccttt tatatcaagt
     4981 tgcctatcac acaaggagga gctgagtcag gaagtatttt tattggtttt caaaagaaag
     5041 aactagatat gattttagaa aatctaacgt cattcaatgg actctcttta tacatgattt
     5101 ctggaacgac aaatcgacta tacacattcc atgataataa aatagctaaa gaaacgtttg
     5161 aaagacaaga aatgatcatc acagagagtc tgaagaaaaa ttcacaattg ccgattgacg
     5221 atctggaaaa aaacaatttt gttcaacacc aagaactgtc tgatgattat cgtatattag
     5281 ctgtactaga taaaaaaagt gtaaaaatcg aaacgggaaa aaatcttgcc ccgctgatca
     5341 ttggtatcat tattttagac aatatactgc ttatctttct ttataaaaca ttcaaacgat
     5401 attcacagca agtatctatc gtcatgcagg ccatggatca aacagcagca gggaatttag
     5461 agacgcggat cgacataaca aacacggaat atgaactgaa ggaactttca atcggtatca
     5521 acgaaatgtt ggatagcatc aatcagtttg tagaagacat ctacaaatta gaaatcaaac
     5581 agcaagacgc acatatgcgg gcgcttcaag cacagatcaa tccacacttt ttatataata
     5641 cattagaata tatccgaatg tatgcaatca gcgaaggaag tgaagaatta gcagatgttg
     5701 tgtatgcgtt ttccgctttg cttcgaaaca atacgaatca agagaaaacg atcactttaa
     5761 aagaagaact ggacttttgc gaaaaatatg tctatttgta tcaaatgcgt tatcctaatc
     5821 gagtagccta tcattttatg atcgatcctg atttggaaaa gatcgaagta cctaaatttg
     5881 tgatccagcc attagttgaa aattatttta aacacggtat tgattttact cgattcgata
     5941 atgcgctaag tgtcaaagtc ttgcaggaag gcaaacgggt aaggatcatc atcaaagata
     6001 atggaaaagg gatgactgaa aaacggctaa aacagattga agaaaaactg tctcatccta
     6061 aagtggaact tcacggttct atcggtctgc aaaacgtgaa tgaacgttta cgtgccagct
     6121 tcggcagcag ttattatatg tcgcttgaga ataatgagac aggtggactg accgtttcta
     6181 ttacattcaa ggagggatga gcgtgtatca agtgttattg gtagatgatg aatacatgat
     6241 tttgaatggg ttaaaaaaga taattgattg gcaaagttta ggctttcaaa tagtagcaac
     6301 agcagagaat gccaaagaag gactagctgt acttgaacag cgacagattg atttagtagt
     6361 aacggatgtg acaatgccag aaataaatgg actggaattt attgaagcgg ctcaaaaaga
     6421 acgtcataat tttgaattta tgattttatc aggataccaa gaattcgatt atttgaaagg
     6481 aggtatgcaa ttaggcgccg tgaattattt gatgaaaccc gtgaataagt tcgaattggt
     6541 tgaaagcctc aagaaaatca aaacacggtt agatcagcaa aatgaacaaa aaaatcaaca
     6601 agaaatctat caagaactgt tattcagtca atggttgaat gaagaattgg atgaaataag
     6661 tgaagaagaa ataataggac aaataagtga gaagcagcat aggatagtat tgattcaact
     6721 ggcaagaact catgatactc ttatcaatag atggttgcaa gaacggcaac aaatctttta
     6781 ctatcaacga aattacggag accgtgtatt gatcacgttg ttgttggagg cagatgaagt
     6841 tgatggcttc tgttgttttg ttcaagaaaa ctttcctgat caggaatggc tgatcagtat
     6901 cggggaagag acagattctt tggacaaaat ccctgaaaca ttccaacaag cgaaagacaa
     6961 tctacagttg catcaatttt atggagataa agacgagcat gttttttacg cagtacaagc
     7021 agaaacaaaa gatcaaacca tcgatttttc aagtttcaga cgcgtgctgc aaaataaaca
     7081 attggaagtc gctcaaaaaa tgatcactga tttttttgaa cagtttcagt tagcagccat
     7141 gatgccggaa gatatacgat attccagttt tttactgttt atggagattc aacgggaatt
     7201 gattgatctg gaagatgagg aatatttaca aggaatcgaa aaaatccatc aggcaaaaac
     7261 ggtgcaagaa cttcagcagc tacttctgtc attcatcaaa aagcatcaga gacagaaaaa
     7321 gtacagtgac aatgtggaaa aagtaatcgt aatcctgcac cagcattatc aagaaccact
     7381 tacactcaaa gaagtgtcag aaaacctgca tttaaatgtc atgtatctag gacagctgtt
     7441 caaaaaagaa acgaaaaaaa gtttttcagc ttatttgaac catttacgta tggaaaaagc
     7501 gaaacaactg cttttgcaca gtaatcaaaa catcaatgag atagcaagtg agattggcta
     7561 caacaacacc acgtatttct ctaaattatt caagaaaatc gtcggcagat cccctaaaga
     7621 gtaccgagaa aacagtggag aatcataaaa gaaaaagcca atgtaatgaa ataacttata
     7681 aaaaattgtc tagaaatcta attttgggct gttcttatta cttgaggcaa ggcagctttt
     7741 tcatgacctc tcgataaacg g
//
LOCUS       DACEUZ010000106         7623 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.106, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000106 DACEUZ010000000
VERSION     DACEUZ010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7623
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.106"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(14..706)
                     /locus_tag="IXG44_002091"
     CDS             complement(14..706)
                     /locus_tag="IXG44_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ1658797.1"
                     /translation="MQIELQHTHDKETFEKNYKVYVELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEIGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            791..967
                     /locus_tag="IXG44_002092"
                     /pseudo
     CDS             791..967
                     /locus_tag="IXG44_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
     gene            complement(1143..1712)
                     /locus_tag="IXG44_002093"
     CDS             complement(1143..1712)
                     /locus_tag="IXG44_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAQ1658798.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1786..2109)
                     /locus_tag="IXG44_002094"
     CDS             complement(1786..2109)
                     /locus_tag="IXG44_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1658799.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2263..3075)
                     /locus_tag="IXG44_002095"
     CDS             complement(2263..3075)
                     /locus_tag="IXG44_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1658800.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            3429..4277
                     /locus_tag="IXG44_002096"
     CDS             3429..4277
                     /locus_tag="IXG44_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HAQ1658801.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            4422..5363
                     /locus_tag="IXG44_002097"
     CDS             4422..5363
                     /locus_tag="IXG44_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HAQ1658802.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKTFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5353..7524
                     /locus_tag="IXG44_002098"
                     /pseudo
     CDS             5353..7524
                     /locus_tag="IXG44_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
BASE COUNT         2272 a         1511 c         1371 g         2469 t
ORIGIN      
        1 agggggggat tttttatcgt tgtggaatat ttaaatacca acgcttgtca tgaaaatctt
       61 gtgctccacc cttagtattt ccttctggat cgtttgtcgc acgcatcatg acgtatactt
      121 tcttattagg gaaatcacgc atattgaaag atacatgata gccaacattt cctaaagtat
      181 tataagcttg atttacgtct ggtctataaa tcccatcagc tcttactcta gctagttctt
      241 ttccagtatt gtagtccata atgaaaatat actcgtattt ataattagca atgtgccatc
      301 cagctacatg caagtttgcg ttttcgattt ctccgaactg atcaatgtgg gcgtaatttg
      361 ttccatctgt cagcgtagaa tttgcagcac ctgctcgagt tggatcaatt actggtttat
      421 cctccgaagt agttggatta tcatccgtaa aaccatgagc caaatcatag gctagttttt
      481 ctttacttac acctatttca gaaagataac cgtaaggatc tgtatgatcg ccccaaatat
      541 tttgtgttac ccataaatgc gatttgattc ctggttggtt ataaggagtg tctaatgtta
      601 atggaatacc atatttcatt gctgaatctc ttgccaattc aacgtatact ttatagttct
      661 tttcaaaagt ttctttatca tgtgtgtgtt gtaactcgat ttgcacagga cttttggctc
      721 acgaggttgt cctactctac atcttgctga tagatatgct gaaaggaaaa caccaacatt
      781 atgttttgct ttttcagcta gaacgagaca agcaggaata cctttagaag atatcaaagg
      841 ttttttaggt cataaagatg tttccactac acaagtctat gcccatattt ctcccgaagt
      901 gaaaaaaaga tcaatgaatc aactagaaaa ctatatagaa gagcaaataa aaaagcactc
      961 aaattgagtg cttttttaag ctcactatca ctaaaactat cactaatttt atttataagc
     1021 gaacatgttt ctatcactga ttttttaaat ctagcgaaaa acccttatct taaaggcgtt
     1081 cgttcaattc tttggcaagg tcttcaaagc ctggcttgcc tagaagtgcg aacatggttt
     1141 tcttatagct catgtagaaa aattttcttg atgatgattt tatccatcaa ttgagattga
     1201 ctgttttgct ctcctatgat ttcagctatg attgtccgat tttgcggttt tcttcttaac
     1261 tggtagttga ttttcccagt ttccatcaac tcgctaagca tatccacgac tccgccaaat
     1321 ccggtctttt tcgtaataaa acctagtgct accattcgtt catctagatt ttttgctgta
     1381 atcgtcacac cagtaatgag cgagtgctgt aatgtttggg ccagctcttc catcccttca
     1441 gcattggata cgacggaagc tgttttgtta ataggttctt cgtttatttc ttttggttct
     1501 ttcctctcca ctttcactgt ttgacgaagg acatctagtt cttgcaagag cgtttcgtat
     1561 tgttcttggg tcagcatcac gccagctact ttttcacgat taaatatata tacgccagtt
     1621 cccgcgtctc gcgcttgagc aaatacatcc atcggcgatc gtttcacttc tgtgatcgaa
     1681 gatgtgggaa cttctaattt ctttaattcc aatggatttc ctcctttact aatcgctttt
     1741 tattatagca cagactgact tgttttgatc ttttttccaa aggttttatc ggtttttgcc
     1801 taaccagtaa ttgatatcgg taagaagtct ttcgatacca aatgaaggat ggtacagata
     1861 cgttttttga tcttcgttat ggtctagtgg aaaaaaatga tcgatgatca ctagatctgc
     1921 ataccgaggc ggcttatttc gagtacatac atgtatagag atccgttttc gatgtcttag
     1981 cgttttgttc agtaccccag caagtcttct tgcttcttgg tgttcatagc gaaaaaaaaa
     2041 gacgattttc acttcaggag ggtggacatc taaataccct cgttcctgga gtgttttcag
     2101 taaaagcatc ccccactcta ctgcggggtg tgcaagtgct tgttggatcg ctagccgaaa
     2161 ttgatatagt ttatctttgg aattgggaaa tttttctaat gtttcctgat aataacgaac
     2221 ttcttctatc agccggtcat ggataccaat tacgtaacga agtcattctt ggtaaagtac
     2281 ttttgtcagc tttgatttgc tttcgctctc aaattcgcta ttcagccaaa acagcgaatc
     2341 aagcttttca gtcacaatcg tcaatttccg atagtcttgt gtctcttcta tccgtttcca
     2401 ccgcttttca gtaatctggt tacagctcaa gcgcaaaatt gctagataat ttttttcttt
     2461 tattggcaaa atctgctcct ctctaaatag caattcagga aacaattcaa agaatagtgt
     2521 tcgactgtct cctaaaatct cgatttctgc cggatccttt ctttgcttca ataattggat
     2581 gaacatctca gaaaaggact gctctccatc atctgcaaaa aaatgcggaa aatctttcaa
     2641 aaagcaaaac agcagatatc ttacttttct ttctgaacca aagatctgtg cttttttata
     2701 agaaaaagaa aggtggtact cgccaaacca ctttctcaat cgctcttgcg tagaacgatc
     2761 gtacttctgc tcgtaaggtt cattcaatag cagatttttc agtaaacaaa acgggggtga
     2821 ttggatcaga aaaacaaaca agagataagg ttcgctatat ccttgatcat aaactaactg
     2881 aacggttttc ttatgaagaa tcagctgaat gggacagtcg ctttccgcca aacgtttcag
     2941 atgccgatcc actgttttaa catcgatttt cagttcttca gctaaatcag cttttcgcca
     3001 gatcaaatct ttcattagat tgattagtga aaataaattc tgtgcatttt ttgataatag
     3061 tttttgccat ctcatcgatc atttctcacc tgatttctaa acatattgtt tgatcaaaat
     3121 gggttcactc ctaaaaagat aaaaaactcc ctttctattt atttaaatac gcaaatcttc
     3181 ctcattattt tttattttac tcaatttaat cgtattctca tttcaaaacg aatgtacttt
     3241 aaaaaaacat aataagatca ctttgcttgt aggatgtatt cccttccatt acaatgaaat
     3301 aaatagcgat tttatgagaa ggtgatgtga aaatggaaga aaaaatagaa gaatccaaac
     3361 aaaaaatcga tcagttgata taagaattag aaaaagcggt catcggcaaa cgttcaacta
     3421 tccaattgat gatcgtggcc ctattagcag gtggtcatgt cttattcgaa gatattcccg
     3481 gtgtaggtaa aacgttactg gtcaaagcac tagcaaaagc tgtacaggga accttcagca
     3541 gagtccaatg tactcctgat ctgctgccta gtgatattct tggtttttcc gtatacaatt
     3601 cacaatcaaa agagtttgaa tttcgtccgg gaccgatctt taccacgatc ttgttagcag
     3661 atgagatcaa tcgtacgact cctcgtactc agtcagcact tttagaagca atggccgaga
     3721 atcatgcaac gatcgacaac aatacatacc ctctagacaa tcattttttt gtcttagcta
     3781 cgcaaaatcc aatcgagtac gaaggaacat atcctttgcc ggaagcacag ctcgatcgtt
     3841 ttttattccg actacaaatc ggttatcctt cttttgatga cgaacttttg ctacttttgg
     3901 ataaattcga aaaagtgttg gataacttaa atgaagtatt gaacagttat gaaatagaag
     3961 aaataaaaac aaatgtggcg aatgtctttg tcagtcccga agtggcatct tatgccttgc
     4021 agttagtgtc agcaacaaga agtcatgctg ccatccagct tggcatcagt ccacgaggaa
     4081 gcctttcctt tattcaggca gcaaaagctt atgctttaat ccatgggaga aattacgtca
     4141 cgcccaaaga tttgcaggat ttagttcctt acgtgttttc ccatcgcctg attttttacg
     4201 atcgttcatt gaaggaagac gaaaagaaac aaatcattca tactcttgtt gcacaaattc
     4261 ctattcctgt gaggtagatt gaggtagaca atggaaaaaa tcaaagatta tttcattaat
     4321 tttgtgttat ttctggtata tttcatcatt gtgctttttg ctcttgtatt caacaattct
     4381 ttaggctggt tcttgttggc cttttttact ctttatgtat catggagctt gatttcatta
     4441 agcagctttc taaagaaact ttcggttact tgtacattgc agccactcgt ttatcgcaaa
     4501 aaaagtatgc aggttatttt ttccatagca aaaaagagaa ctttttcttt acctctccct
     4561 cgtttacaaa tcggcttccc agcaatcttt gcagaaaaaa acaagagatt ttgattctta
     4621 caaataaacc gcaagaagtc caattgcttt ggcagccaaa agaaagagga tgccatgaaa
     4681 cattaactgt catttttaca gcctatgata gtctaggact tttcagaaaa agtgcaaaaa
     4741 gagagatctc ttttcctgtg actgttctgc ctgctttttg taagattcaa gcagaaaaat
     4801 tacagagagt tctggaaaaa aaacagcagc tgaacgcgta ccaaagaggt cttgattttc
     4861 gggaacatcg cgcttatcgt ccaggtgatt ctttcaaccg aatcgattgg aaattgtctg
     4921 ctcatactca agagtggtta taccgtgaat acgattatca agaagaagaa tgttctcctc
     4981 ttttttgctt ttggggttcg cccagtccaa aatttgaacc tctgctagac ttgtattttt
     5041 ctttatggca tttgagaaaa gaaaagcagc cggaacttct gatattaggc aaccgcgcct
     5101 ttcatggcaa agaccccggt catacctatt ttgcttcttt ggaagctggc gatgagaaaa
     5161 cttttttcgc acacttgaaa aaatatgcaa aaaaacaaat cgtcctcttt gttcctgaga
     5221 attctcctga agtctcagaa gctattgaaa cattgtcaaa agatcgaacg gtttatcttg
     5281 tttatttcgt agaaaaacaa ctcatggtcc aagaaaaaga caagatatgt tcattgccag
     5341 gaggtgaatg gattgacgat taaacgggaa aaatggctgc gtggcgttta ctcctttgtg
     5401 atactaagtg taggtgctcc gatatttgta gtagctaatc aattgcaaaa cagctacctt
     5461 cttatcctaa ctattggtgt cctatgcctt attgtctgct gtattcctaa aaaatgggta
     5521 cgtttgctgc tttctttgcc agtaatgata ggttgtttgt ttttatattt tccttcgaag
     5581 acattttcca tattatggat cttacagttt ttttcgtcag ttacagagga attcttccat
     5641 ctatcccaag gtaaacttta ttatttgcca gaaaagacag ctttttttct cataatgttg
     5701 ctgatctatt tatttattac attgaccgtg gattacgact attggtacgc accctttctt
     5761 tctttgatgg cttacttcat gatgcttacc gtttttcgga aaaaagattt actgtacgaa
     5821 atgattgcgg tagtaactgt tttgttcgtg tatctggcag caaggcaatt tttctccatc
     5881 cggttgttgt cgagaaacac tcgattccag tcattgacta cagttctgct tatttgtttc
     5941 ttaactttta gcgctatctt gcctttatat cttccgaaag tacaccaatc tctcgaggaa
     6001 acgagtgaac cgatccgaga atatcttaat gatgaagggc tttatgacac actccatgag
     6061 tatcaattaa ccggaagtgc aagaacaggc ttcagtgaga atgatgaaca gcttggtgga
     6121 cctttatatg ataatggcga agttgttttt acagcgaacc aaaaaggaaa tggctattgg
     6181 aagatcgaaa ataaaaatca ttacaccggt tctggatgaa tagaagatca ggacgcaccc
     6241 atccaaccaa tccaagaatt ccctgttgac atcacgagct accaaaccgt tatagaagac
     6301 aaagaaaaca ttacattgac cctaaaaaaa caagctttct taccttatcc ttatggaaat
     6361 actaccttga ctgatttttc attagctagt ggacaaacgc tttactattt gcctgaaagc
     6421 cagcgattca ttcctgtccc gaatgaatcg acacgttctg caactttaca aacagaaagg
     6481 ttagttttta cgagagaaga attaaaaagc agtcagttaa aagctgatga tttagaaaat
     6541 cagcagacaa atcttcaatt gccaaaaagt ttgcctaaac gtattgagga cttggcggtc
     6601 gaattgacca aaaatgaagc aactttaatt cgtaaagtcg aagctgtcta acagtattta
     6661 aaaacaagcg gaaatttccg ctattccaaa acggataccg ggcatactcc aaaagaacac
     6721 gattatgtgg attatttcct ctttgattca aaagtcggct attgtgacaa cttttcaact
     6781 tctatggtcg tccttcttcg aacacttggt atcccagctc gctggacaaa aggttttaca
     6841 aatgggctca gaacaggcac agataatcaa gggaatgagg aatacagcat tctcaatagt
     6901 catgctcatt cttggccaga ggtatacttt cctggatttg gctggcttcc atttgaacca
     6961 accccttcat ttgccaatcc aatgggttca caaccagttg ctgaaacaac cgaaaccgtc
     7021 cagcctgcta tcgaacaacc aaatgaaagc gaaaatatgg cagaaactat ttccagtgag
     7081 agatctgagg tcattccatc agaagaaaca acactcacac cagcttataa aatcgtctta
     7141 ttcagccttg gagtgtgttt atccgtcttg ttgttctttc tattgttatt cagaaaatct
     7201 ctgcgctgga caagttatct tatctatctt catttttttg cttctactaa tacgattgca
     7261 tatcaaaaac ttcttaaccc tttggaaaca ttggtaccgc gcaaacccgc agaatctttg
     7321 caggaatata gcctgcgaat agaacaagaa ttcaagctag atctgcgttt cattcgatta
     7381 actactcaat atgaagcaga aatatatgga cgacaagcag atccggtaaa attcgataaa
     7441 aaaacctatt tagaaataac gcatataata agaaaaacgg tatacaaaaa aattctgaca
     7501 aaagggagcc gcttcaaaaa ataagataaa atttctggag aatctacttc tcgaatgccg
     7561 agaaccagct gattgagtat cgattactcg aaaaaaaggt tgtgacacgt tttttgtcac
     7621 aac
//
LOCUS       DACEUZ010000107         7622 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.107, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000107 DACEUZ010000000
VERSION     DACEUZ010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7622
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.107"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(137..1147)
                     /locus_tag="IXG44_002099"
     CDS             complement(137..1147)
                     /locus_tag="IXG44_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ1658803.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(1159..2112)
                     /locus_tag="IXG44_002100"
     CDS             complement(1159..2112)
                     /locus_tag="IXG44_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1658804.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            complement(2102..3172)
                     /locus_tag="IXG44_002101"
     CDS             complement(2102..3172)
                     /locus_tag="IXG44_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1658805.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            complement(3178..4221)
                     /locus_tag="IXG44_002102"
     CDS             complement(3178..4221)
                     /locus_tag="IXG44_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1658806.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            complement(4222..5163)
                     /locus_tag="IXG44_002103"
     CDS             complement(4222..5163)
                     /locus_tag="IXG44_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1658807.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            5247..5594
                     /locus_tag="IXG44_002104"
     CDS             5247..5594
                     /locus_tag="IXG44_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HAQ1658808.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            complement(5664..7325)
                     /locus_tag="IXG44_002105"
     CDS             complement(5664..7325)
                     /locus_tag="IXG44_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ1658809.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
BASE COUNT         2130 a         1574 c         1379 g         2539 t
ORIGIN      
        1 tttttctgct attgttttat tggtttgtca taattagtga gcttttcttc aaaagaaaaa
       61 ttcctagaaa caaaagtcat taagactttt tattaacaag gctaacccta acgctcctat
      121 cagtttcatt cattttttat cccttgataa ccgaatgtct gcccattttt tctactactt
      181 ggctctttct tacggaattg ctttcttctg ctccgtcggt attgatcaaa agaaattttt
      241 tattatacca ttgggactta taacgcaatt ttgttattaa tttatgccag tcgtgaatag
      301 aaaaatcagc atgtgcctct tcttttggaa aagattctac aaataagtag tcatattgtt
      361 tgaccaattt cccagataat tgttcaagat aatcttctcg ctggttcttt tgtttcagat
      421 aaagatccat tacttttctc ttttgtttct gataattgct tgcctgatct aacctgatct
      481 ttctatggtg agcggagcgg actttcacat tcaatttccg ctgtgcatgt tgcagctttt
      541 cttctgtctg gtgaaatttt ggtaaagtta cttctagagg tcgagatgtt tggatcaatg
      601 tcttcggatg ataggcgatc ccgatccatt tagctaaaga ttcttttttt actggcgaaa
      661 catcacaaag tagtgcgaca taaaattctt cattatatcg agcagaaatc gtagcggagc
      721 gaatcgatcc ttcaactggc cgatgctgat ttatcttgat tagttctttt tgttttggta
      781 actttaagta gccattttcc agatagatcg tacgggtttg gttgttggtc gtataagatt
      841 gccacatact ttttttcgtt ttaagctttg ggaagctggc acgtcccttg aaataattgc
      901 gaaaagcccg atccaaatta agctgtgcat ttgctaaagc aagactgtct acttctttta
      961 agaatgggta ttcttttttt aatgtagcgg gtgtgagcga aaaattggct actcctgttt
     1021 gttgccaact tttctgccgc tttttcagca aatggttata ggtaaatcga gcacatccaa
     1081 aagtacgaat caattgctgt ttttgttctt ctgtcggata catacggaat ttaaaagctt
     1141 ttagcatcta tgtccctcct agttagcaac cagaagagat tccttttgtc gttttagttt
     1201 ttttgcgtaa tatgggactt cctgctcagt tgcatagaca aaatgttctg gtgcgatttc
     1261 acgaagttga cgtacttgtc cgtcatctgg ttcaggttga tatttgattc ttctgcgttt
     1321 tctctcatgg tctggatctg gtagaggaat agcagataac aaactttctg tataaggatg
     1381 aacaccgtga ttatagattt catcacttgt tcccatctct aataacaagc cgttatgcat
     1441 tacaccgatt cggtcactaa tatgtttaac catcgacaaa tcatgtgcaa taaatagata
     1501 ggtcaattga tgttcttttt ggagatcttg caacaaattg accacttggg cttggatcga
     1561 cacatctagt gcagaaattg gttcatcaca aatgataaaa cgcggtttta ctgccaatgc
     1621 tcgtgcaata ccaatacgct ggcgttgccc acctgaaaat tcatgaggat aacgagtacc
     1681 gtggcttgga ttcaatccaa cagttctcaa tagatcatcg actttttttg cacgctcttc
     1741 agatgattta gctagtccat taacatcaat tccttcggca atgatatctc tgactttcat
     1801 acgaggattc aacgaagcgt atggatcttg aaagatcatc tgaacatcat gccggaattt
     1861 cgtcaaacct tttttatctt tgatcttcgt tatatcttga ccgtcgaata atatctcacc
     1921 atcagtaggt tcattcaagc gaatgatcgt acggccagta gtggattttc cgcttccaga
     1981 ttctccaact aaaccgaatg tttctccttc ataaatgtga aagctgatgt catttacagc
     2041 atgtacttca ttcttttttc ctacattgaa atactgtttc aatcctttaa cttcaagtag
     2101 tttatttggc atcagcaatt cctccttgac cgtaagtatg tttttgtttc tctttaaaga
     2161 tagcccagcg acgttggatt tcttctggcg gtgtcacttt gggtgcctgc ggagcaagta
     2221 gccaagttgc tgccttgtgt gtttttgata gttcaaaaaa tggtggttct tgttctgcat
     2281 cgattttcat cgcgaattca tttcttggat agaatgcgtc tccttttggc gggttcaata
     2341 aatccggtgg agaaccagga atagcataca atcgatcgtt cgcactatct aatgtcggca
     2401 ttgaaccaag cagtccccaa gtatagggat gttgcgggtt gtagaaaatt tcttccgatg
     2461 ttccaacttc aacgattctt cctccataca tgacagcgac acgatcagcg acattcgcta
     2521 ctacaccaag gtcatgagtg ataaagataa ttgatgtatc gatttttgtc tgaatttctt
     2581 tcagcagctc caaaatctgt gcttgaatag tcacatcaag cgctgttgta ggttcatcag
     2641 caatcaggat ttccggataa cataccagtg caatcgcgat cacgatccgt tgacgctgac
     2701 ctcctgagaa ctgatgcgga taactgttga tacgtttctc agcttcgggt attccaacta
     2761 atttaagcaa ttctaatgca gctttttgtc cctctttttt tgaaacttta ttatgctttc
     2821 taagggactc agcgacttgc ttcccaatgg gcatcgtcgg atctaaagag gtcattggat
     2881 cttggaaaat catcgctatt tctttaccac ggattttttg catctctctt tctgattttc
     2941 caacgatatc gttaccttta aagaggattt gaccagcatc tatattggcg ttgcttgaca
     3001 gcaagcgcat gatgcttctg gtcgtcactg attttccact tccagattcc ccaacgatcg
     3061 ccaatgtttc tcctttattc aaatgaaaat tgacaccgcg aatcgctcgt acctttccag
     3121 catacgtatc aaaagagatt tctaagtctt tcacttctaa aattttcgtc atcgtcttca
     3181 ctctttcatt ttaggatcaa aggcatcacg caatccatca gcaagtaagt taaagccaat
     3241 catgataacc gataaagtta ctgccggaat ccataataga taaggcagat acaggaatgt
     3301 cttgtaccct tcgctcaaca tcgttcctaa tgaagctgac ggaggcgtaa gacctaaacc
     3361 gataaagctt aaaaaggctt cgaagaaaat agcagatgga atactaaaca tcatttgaat
     3421 aataatgaca ctagagatat tcggcaaaat atgtttgaat gcaattttca ttcttgattc
     3481 acctaatgtc aatccagcta aaacatattc ttgatctttt aatttcattg tttgtgcacg
     3541 aacgatacgt gccattggta tccagtttgt gatggccata gcagccacga ttgaaaaaat
     3601 tcctggttca aaaacgacca acatcaaaat catgacaacg aggtttggaa ttcctgataa
     3661 gatttccaaa aaacgttgca tcacgttatc gacacgtccg cctaacaaac cagaaaccaa
     3721 cccgtaagct acaccaatcg ttacatctag taatgcagca ataaatgcga tcagcaacga
     3781 gattctcatt cccataaata agcggcttaa tacatcacgt cccaaaccgt ctgttcctaa
     3841 aaagaaattt acgtttgaag ggacgtttgc ttgagcatat ttatcaacaa gttcaccacc
     3901 aaccattgct tttccattca atccatcgat attgattccc ggaatacgag gaggcaaatt
     3961 gatataagcc acgttttggg cagttggatc atgaggagaa acaaagattg tgatcacaga
     4021 gatcaaaatg atgaccaata gtaaaaccat tgaaataacg gcagctttgt ttttctttaa
     4081 acggcgccat gaatcttgga taaaattcaa agaaggtgca gtaatctgtt cacgttcttc
     4141 ttcggtattt ttttgtaatg gaagaaattc atccgcaggg atatctgcaa ctgtttcata
     4201 acgctttggt atcatttcca tttatcctcg gctcccttct gaaacacgaa ttcttggatc
     4261 aactagaccg tacaatagat ccacgatcaa aataacaaaa atcagcataa atgagtaaag
     4321 gatcgttaca gccatgatcg taggataatc atttgttgtg attgacttaa cgaattgttc
     4381 cccgatacca ggaatagcaa agatattttc taccaccagt gatccggtca tcaatgctac
     4441 agctaacggt cctaataaag tcatcaatgg aatcaaacta tttcgaagac catgtttaaa
     4501 ggcgatttcc caacggctca atccttttgc ttttgctaat tccacataat cactatgcag
     4561 tacttccacc atttccgtac ggataaaccg agcagagtca gcaagcggcg acatggccaa
     4621 cgcaattgtt ggtaagattg tgtacacaaa gccatcccat tttgcgattg gcaaaacttg
     4681 taatttgatt gcaaagatat attgtaataa aacagcaaat acaaagtttg gaatggaacg
     4741 acctaatatc gctacgagtg tagaagatgt atctgcccat gtattttgct tcatggcaga
     4801 gatcgttcct aaaatcgtcc ctaggactgt tccaaagata attgcctgta accctaactg
     4861 caaggatggt ccgattcttc cagctaacaa atgtgcgact ggctggtttt taaattggaa
     4921 ggaaattcca aaatctcctt gaagcaaatt agaaagataa atcccatatt gtacgataac
     4981 tggtttatcc aaaccgactt gtttatttag catctcgatt gtttcaggac tcaaacgttc
     5041 ctgattcgta tatggcgtgc ccggcagcaa ttgcatcaag aaaaaggtga ttgttgcaat
     5101 taaccaaagc gtgatgatca tgaaaaagac acgttttaat acatatttga tataactatt
     5161 cactaatgtt tccccctagc ttttgacttc tgacagatgt ttcgctacat tattagataa
     5221 tgaaccaaaa ataaaggagc aatcaaatga aaaaaaaatc tatcccctac gcagtagctt
     5281 ttctactgat tctggttatt ttaataaaaa atctcatcaa ccattccttt acactcattc
     5341 aattatctaa tgatctattc ttatggtcat tgccgtttct gattatcggc ggttttttat
     5401 gggtcttttc ttcgggtttc ttcgatcatt ttcagcgatc ggtccatctt gcacgaacaa
     5461 ggaaccgaaa gaaaaaacca gaattttcct cgctctcgtc tgccagctac ggtatgtaca
     5521 gcttttggct gatcattgcc ggaatactaa ttgctctttc tgctattttc atgctttttt
     5581 ctttgttagg ataagtgaat atgagaacga ggttgcgaca tttgccgcag cctcgttttt
     5641 tctcatgctc acggtattaa cgattattct tcaacgtatg tccatttgta atcccatgaa
     5701 gcgcctgccg catggtgaac gatacctttg attttttcat tcactaggtg accttctgct
     5761 ttttggtaca ccggaacgac acccatatca tcagagatga tcttgattgc tttttggaaa
     5821 tcatcccaac gggcttgttc atcgccagcg tctgttgatg aagctgcttt aactgcttcg
     5881 tcatattctg cattgctcca gcgaccacgg ttgtatgaat tacctgtgac aaacaaatca
     5941 gtaaagctgc ttgggtctgc atagtcagca gcccatccgc ctagtactgc gtcaaaatca
     6001 cccgaagttg agcggtcaag acgtacagag aatggaacag gtgttggttt gactgttacg
     6061 ccatccaagt tttcttggat tgagttttga acatattcga ttactttttt cgatgaatca
     6121 tcatcagaag ccatcaaatc aaattctaaa gaatcaacgc ccaattcttt tttcgctttt
     6181 tcccaatatt cttttgcttt atctttgtca taagaaacta attttccagc ttcttttgca
     6241 aagtcttcgt tcgttgttgg actttttacc atatctgatg ggatcaatcc tgtagagaca
     6301 actgatccgt cacctagtac ttgttttact aaagcttcac gatcgataga ataagaaata
     6361 gcttttctta ggttcacatt gttgaatggt gaattctttt cacgttgatt gaattcgata
     6421 tagcttgtcc gcgcttcttt aacagaagta taagctggat cgtttgcgtt ttgctgtgct
     6481 aactcaccag acaagatcac gtcatctgct tggccatctt tgaataagtt caatgcagta
     6541 gaagattctt ttactacact gactttgatt tcagaaagtt ttactgtatc tttgtcccag
     6601 taatgatcat ttttcttata agtccattct gtatctgttc ctggtccatc aaaatcttct
     6661 aatgtgaatg gtccattata aacagcttta tcacttgcag atgcataggc atccccattt
     6721 tcttccacta cggattgttt ttgtgggaaa aatgatggga aagcaagcag atattggaaa
     6781 tatggtgttt gtttcgtcaa tttgatttct aattcatagt caccaactgc tttgatccct
     6841 agttctgatt tatctttctt tccagctgta atgtcttctg cattttcaac tggcgcatac
     6901 aggtaagcat attcagaagc tgtagctggg tctactgttc gttgccagcc ataaacgtaa
     6961 tccgctgctg taacaggatc accatttgac cattttgcgt cttctcgtaa tttgattttg
     7021 taagttaatc cgtcttcact gacttctgcc aactcgctag ctcctgccgg ctgtggtttg
     7081 ctgtcttcat caagacggta aattccttca taaacgttat tcaatgctgt aaaactgatt
     7141 gtatctgttg caactgataa gtcagctgta ggcatttctt gaggcacgac taagttaaat
     7201 acgcctcctc catctgctga accgcttttt gctgaactac tgcttgtggc tgaactgccg
     7261 ccataacatc cagcaagtac aaggccgcat accgcaacca aaccaaatgt aaatttcttt
     7321 ttcattttac gtaccccttt attggaattt tcagaataat taatattttc tgaagttgtt
     7381 taagagttta atgaataatt attaaaaaat caacactttt ttttgtcttt ttcataaaat
     7441 aatgattttt tcgtgaggtt tcaaggaatt tttcagaaaa aatagctttg tacaaggttt
     7501 tccatggtct tccaaaagcc attaaataat tttcgttata ttgtctatta ttgccataca
     7561 aagataaaaa agaataaaca aaaagaaact gtgacaaaag tcttgtcaca gtcccttatc
     7621 tg
//
LOCUS       DACEUZ010000108         7597 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.108, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000108 DACEUZ010000000
VERSION     DACEUZ010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7597
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.108"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(135..425)
                     /locus_tag="IXG44_002106"
     CDS             complement(135..425)
                     /locus_tag="IXG44_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1658810.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(543..803)
                     /locus_tag="IXG44_002107"
     CDS             complement(543..803)
                     /locus_tag="IXG44_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1658811.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(940..1353)
                     /locus_tag="IXG44_002108"
     CDS             complement(940..1353)
                     /locus_tag="IXG44_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HAQ1658812.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1809..2294)
                     /locus_tag="IXG44_002109"
     CDS             complement(1809..2294)
                     /locus_tag="IXG44_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1658813.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2433..2648)
                     /locus_tag="IXG44_002110"
     CDS             complement(2433..2648)
                     /locus_tag="IXG44_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HAQ1658814.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2872..3672
                     /locus_tag="IXG44_002111"
     CDS             2872..3672
                     /locus_tag="IXG44_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ1658815.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3691..5559
                     /locus_tag="IXG44_002112"
     CDS             3691..5559
                     /locus_tag="IXG44_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ1658816.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLKLAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5552..6043
                     /locus_tag="IXG44_002113"
     CDS             5552..6043
                     /locus_tag="IXG44_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HAQ1658817.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6031..6585
                     /gene="srlA"
                     /locus_tag="IXG44_002114"
     CDS             6031..6585
                     /gene="srlA"
                     /locus_tag="IXG44_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174078.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HAQ1658818.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6603..>7597
                     /locus_tag="IXG44_002115"
     CDS             6603..>7597
                     /locus_tag="IXG44_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HAQ1658819.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2708 a         1213 c         1442 g         2234 t
ORIGIN      
        1 gttactatga ataacataat aagaaagtaa agtggattga tttgagatct ttttatactt
       61 caaaaaagat aacacccaac tttcaaaaat atgttttgga agttgggaca aacgagttaa
      121 tacattttaa tacattattt tttattcaaa aaaaatagca caatggcaaa gatcgtaaga
      181 gagatcataa gccccaatga atgactaaaa agaaaagcag aatcacatgc catatcatcc
      241 catatttctt caagaaaata aataagcggg aaaaagggaa ggaatagtag aacatcgatc
      301 cagcaccatt tttttcttct tttgaaataa cataaaacaa taaacatgaa cgtgcaggga
      361 agtaaaaaca aagctttact cataactaac aaaataccag ctccttttat ttttttctcc
      421 cccacattca aagagtaaca atggtatgta cttaataata gaaaaatgga tcaacttgta
      481 tgaataacag ctgaaaagat ctgcaccaaa taaaaaactt ccagccaaaa ctgactggaa
      541 gtttattttt ttctgaaata ttctaacaca aatatgaaaa agatggccga agctaataag
      601 ccgatttcgt agcaaaacaa tgagatacca gagtcgtaat aataaaatcc tctaatctta
      661 tggataaaat agatcatcgg acaagcagat aagagtaagt atttgttagg ccttttgaat
      721 aagtaaaaga aagcactggt cattatgata aatggtatcc aaaacaaaag tttacttaag
      781 acaatcaaat gatcaactcc catatttttt accaagagag atcataacat ctttatcgta
      841 aaaacaaaat atttttttgc tcattttgaa aatttaaaca tactcgttct tatccaaata
      901 tataaaaatc gcccctcatt catcgagagg cgataagttc taatttaact caattttttt
      961 tgtttctttt ggctgataag ctgttttagg taataccaat ttcagcacac cgtttttaaa
     1021 ggttccagtg attttttctt catcaacatt aggaagatag aatgaacgtt tgtaagagac
     1081 cgaatgtctt tctttccgta catagttgcc atcttcttta tcttctgtat gggactcatg
     1141 attggcggag atcgtcaaag tattatctga atattcaact gtcgtgtctt ctttgtcaca
     1201 gccaggcata tcagcagtta gtttgtattc tttttcattt tcgactaaat ctacttttgg
     1261 ataacttgaa acacctaaaa aatcattgaa tgctggacta aacacgtcat tgaaatcagg
     1321 gaacatgtcg cgaatgctag gtacatttgc catttttgat catcctttca ataagtattt
     1381 tttatttatc cctatttacg taataactct attttgaaaa aaatgcaagt ataatggtta
     1441 gctttgtgaa tatttcatct aatacgatac aaaggctatc tttctaattt gaaaaaggga
     1501 tcattaaaat aataagaaga aaatatgtca aaaggtaaaa aagtaaattc ttctatccaa
     1561 atatagaaaa attgaatcta gaaaaccgcc cgacaataaa tttcattaac tcagttgacc
     1621 gaaaatgaag tagataccaa aaatttatca agagagcaag tagagaagtg agtatattca
     1681 taagagaaat agctgtaacg aattattagg ttaatgcaaa tgcttattta tactccgtat
     1741 aactttctca gaaaaaatga taccaataat gacttgctag tactgtaaat ccgtataata
     1801 tcatctaatc aaaatggtgt tccatttgca tctgtcatcg taatcgatcc ttcgggtgac
     1861 actcgataaa aaccaacgct tccagatcct ccttgggaac ggatttgttt gctggaagct
     1921 gtgaacaaat aatctcgatc aattttttga taaaagctaa taacaatatc gtcactttgt
     1981 aattcggaac ggtgggcaat aagaaataga agtgctttgt cagagttttc taatggaaga
     2041 gaaggtacag tgctttcttg catttcagat gttgaagaaa tgctaaacga ctgaatactt
     2101 gattggctat gttgcgaatc tacattacca gtagaagtat tagtaatgga agtagtagaa
     2161 gtacttttag ttgaaccaac ctcagttgtt gaaatggctg ctgaagattc tttaacagaa
     2221 ttctttgttt tatatccaca ggcagaaaaa agtattacag ttcccaacaa gcctgcgtaa
     2281 atccctttat gcatgatagc tcctcctaaa atagattatc tttattcagt atatcactta
     2341 aacaaagaaa agcagatggt ttaaaatgaa aaaaccttcc caataaaggg aaggttcatg
     2401 aagataataa cttttttaat aattatagct ttctaatatc tttcatcaac ttttctagta
     2461 atcattggga aaaatttcaa gcagtagatc gcacaaatac ctacgacgat ataaatgatc
     2521 ttcgcaaaaa tagttgttga accaccagca atcatagcta ctaaatcaag ttcgaacaag
     2581 cctaccaaca gccagttcaa ccctcccaca atcaataaag ttaatgcaat agcatctaaa
     2641 gttttcatta gaattctcct ttcttaaatg ataaaacaat tatatatcaa aaagagaaac
     2701 attgcttata taacgctcag gaattttagc aatctatgca aaaagatgaa aatttttttt
     2761 gctcttccat tagtacacat tttttagctg aagctgtaaa tttcatgtct taattttttt
     2821 tagtgagctt ttatactgga tttgtaagaa aaaagaagga gaggatcaca tatgtcggat
     2881 tggttggata ttgcaggaaa agtagtaatt gtaactggag gttcctcagg aattggaaga
     2941 agtatcgtcg aaaatttgtt gaagcagaat gcacaagtag cgaattttga tgtaactgaa
     3001 tgccgtatac agcatgaaaa cctactatct ttaaaagtcg atgtgtcttc taaaacggac
     3061 atagaagaag ggatatataa agtaatgaaa catttcaaga cgatcgatgg tctagtcaat
     3121 aatgcaggaa taaatattcc ttctcttcta atagatagaa atcatccaaa aagcaagtat
     3181 gaattatcag aacaagtttt tgataagatg atcgctgtca atcaaaaaag tgtatatttg
     3241 atgagccaag cagttggaag aatcttggtg caaaagggat caggagtgat cgtgaatctt
     3301 tcttctgaat caggattgga aggatcagaa ggacagagct gttatgctgc tacaaaagca
     3361 gcgatgaaca gttttactcg ttcttgggca aaagagttag gaaagaggaa tgttcgtgtg
     3421 gtaggtgttg caccagggat actggaagaa accggtttga gaacacaaga atatgaagaa
     3481 gctctttctt atacgagagg tataagcgtc gaacagctga gaaacggtta cagtcgaaca
     3541 agtacgatcc ctctaggaag gagtggtaag ctgcaagaag tggcggatct agtctgctat
     3601 tatctttcag aaagatcaag ttatatcact ggagtcacga ctaatatttc aggaggtaaa
     3661 acaagaggat aaaggatagg ggggacgcac gtgacgctag taaacagatg gtaccaatta
     3721 ttgaaattac tagtcactca caagaaagtt tcaatccagg aagtgcaaaa agaaatcaaa
     3781 accagtaatc aaaccataaa aaaagatatt ggggagttga atgaagaaat tgccggtatt
     3841 gcaaaaatca tccaaaaaaa caaacatttt gaacttgaaa tttatgattt tgattttttt
     3901 gatgagatca tgcaaggaaa attgaaaaca gccagtgatt tcaattcggc atccaaaaga
     3961 atcgcttatt tactaaatag gctgatcgaa acagaggatt tcttacgtat ggatgatttg
     4021 tctgaagaag tcggtgtcag cagaggaaca gtggtcaagg atctaaagac attaaaagag
     4081 atgatcaatg attttgatgt aaagattaca ggcacaccaa ataaaggact aagaataata
     4141 ggagatgagc tagaattacg actactttta ttttattttg tttctcccta cttttcagag
     4201 acgaaaacag aacagtttct agaagaaaaa caagcaatca aacattttca gaagaagacg
     4261 aatgctagta aacaagaaat caccctttta acgaaagtta tcggtatttc tttgaataga
     4321 atctctagta actatctgtt gaagaagccc atcaatcgtt acagtgggtg ttttgaatat
     4381 atccaagaat tcaatgaact gatcaatcag ttggaagaaa tctatgaact aactttgagc
     4441 caatatgaaa aagattttat cagttttcct ttgaacatca tcaacacaag tataacgatg
     4501 ataaggaaag ctaactctga tttgcgctta tatttcgacc ccatgatgat gcgtattcga
     4561 cgattagatc tcattgatct ggatgagtcc ttcttatatc aagagatgaa acagcacctc
     4621 tctttattga taaatcgggt aatttttcgt tatcgattga ctgatttatt ttatggtgag
     4681 atagaaaaaa agtatccttt ttcttaccaa atcgctagcg aatgcataca agcattagaa
     4741 gctatcttgc aacataagat ctctaaagta gagatcagct acctaacgtt gtatttgaag
     4801 ttagcatttc gatatcaaaa agagcatttt tcctctaaaa aagtagccat tgttcatacg
     4861 acaggaaaag gaacagcatt aatgatccaa agacaaatca aaaggatatt ggggagggat
     4921 atacaaataa cacatgtttc agaagaacaa tatgaaaaag tagatctaaa caaatatttc
     4981 gctgttttta caacgattcc gttaaaaaat gtaaatgaag agacaccggt gatccatttg
     5041 actaatcttt ttgatgactc ttggttaagg gcacaatggc aaaaagcaaa aaaaaagcga
     5101 ttagccgggt ttcattctac tgtttttttc tatcaaatat ggaaagaaaa aaagacgtat
     5161 caagaatatt taaacaaatt agtcgctcct ttgatcagag gctattatgt ggatgaagaa
     5221 tttgaaaaaa ggatcttgaa aagagaatat cagcaaagca catttgttaa caaagaattt
     5281 gcttttcctc acacaaccaa ccagctatct caacacattg ttttatcagt agggctaata
     5341 actgatcaag gtttagcaga aacaggagga aatggagtta tttttttatt tgccatacct
     5401 gaagaaatga cggctgaagt tgagaatgaa ttatttgagc tatacgatat cctatttgaa
     5461 ctcataaaaa tgggaataga tgagtttatt gaattagcac cgacacaaga atcattttta
     5521 aaactaatca aaagtaaagg agagagactt catgaatgat atgtatctat tcatagtttt
     5581 cgcagcttca gcttatgtgc tgcaagcagt tatcagttgg aagcaactaa agcatttcaa
     5641 tcaaacatat agcgaactgc gaagaaaagg aaaagtagcg attggtaaac gacctgggaa
     5701 aatccgttca ggcacgattg ttatgctagc tatagacgat catgatcaag ttttagaagt
     5761 cagaaaaatg caaggggtta catctttggc aaaatttcat tcattacctg tctatgcggg
     5821 tgaagatatc cattacatcg atcgttacca tccctcagtt cgagcggaag atcgtttgac
     5881 acaagcagct ttagaaaatg ccagagaagt atacttacga atagaggcag gaaatgaaaa
     5941 aagtgaacaa ccttcattgc cttttattgc tatgctcaat aattttcgga tatggaaaag
     6001 tacgatcaaa gaaaaaagga gtgacaaacg atggatatca taacaaaatt tgctgaggga
     6061 tttatgaggc tgttccaaac aggtgcagaa acattcataa gctggatgac aggaatcgta
     6121 ccagttgtct tgatgctgat ggttgcaatg aatacgctga ttgctttttt aggtgaagac
     6181 cgagtaacga aagtagccaa attatcagcg aaaaatccgc ttactcgtta ccttgtgtta
     6241 ccgttcttat ctgcttttat gttgggaaat cctatgtcat ttacaatggc tcgtttcctt
     6301 ccagaatact acaaaccaag ctattatgct gctcaagctc aattttgtca tacgagcaat
     6361 ggtgtattcc cccatattaa tcctggagaa ttgtttgtat ggatggggat tgctcaaggg
     6421 atcgaaacct tagggctaaa ttcgatggag ctggctatcc gatacttgct tgtgggactg
     6481 ctgatgaact ttattggtgg ttggataact gattttacta ctggatttgt atgcagacag
     6541 caaggaatcg ttcttagcaa aaaagtagag ctttcagttg attagagaaa ggagagtttt
     6601 aaatgtatgg tgatattaag atcgtaagag gaaacggcgg gtatggaggt cctttgatta
     6661 ttaggccgac aaaagataaa cacaagttca tctatgtcac aggtggaggt gagaggccaa
     6721 tgatcgtaga taaaatagaa gaactgacag gtatggagtc tatcaatggg ttcaaaacat
     6781 caatacctga tagtgaaatc gcgcttgcaa tcgttgactg tggagggact cttagatgtg
     6841 gaatttatcc taaaaaaggg ataccaacca tcaatatcgt ttccacagga aaaagtggcc
     6901 cattagcaca atatatcact gaagatattt atgtttcagc agtagggtta gaacaaatac
     6961 agccactaat ggaaactgat gataacaaag aaacagtaaa tactgcatct ccagataaaa
     7021 agtacaaata tgatacttct aaaaagataa ccgagcaaaa agcagagcag caaaactgga
     7081 tagcaaaaat tggaatgggc gcgggaaaag tagttgctac attcaatcaa tcagcaagag
     7141 aagcagtaga tacgatgatc cggacagtga tcccttttat ggctttcgtt tcccttttga
     7201 ttggtgtgat acaaggttct gggatcggta atgtatttgc taacattatg gcacctttag
     7261 caggaaatgc agttggatta acaatcattg gctttatttg ttcactgcca tttttatctc
     7321 ctttgcttgg tccaggggca gttattagcc aagtgattgg aacattgatc ggtgtagaga
     7381 ttgggaaagg aaatattcct ccccaattag cattaccggc actatttgct atcaatactc
     7441 aaaatgcatg tgattttatt ccagtcggtc taggtttgga agaagcaaaa gctgagacag
     7501 tagaagtagg tgtaccatca gttctttatt caagatttct taacggcatc ccacgagtta
     7561 tcattgcttg ggtagcaagt tttggattat atagctg
//
LOCUS       DACEUZ010000109         7587 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.109, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000109 DACEUZ010000000
VERSION     DACEUZ010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7587
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.109"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(5..613)
                     /locus_tag="IXG44_002116"
     CDS             complement(5..613)
                     /locus_tag="IXG44_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1658820.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLITAYSEKVDKKVVADRLAEWKKTNEQVKKTTVSSQK
                     NESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(772..2169)
                     /locus_tag="IXG44_002117"
     CDS             complement(772..2169)
                     /locus_tag="IXG44_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ1658821.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(2201..3172)
                     /locus_tag="IXG44_002118"
     CDS             complement(2201..3172)
                     /locus_tag="IXG44_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1658822.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            3546..3971
                     /locus_tag="IXG44_002119"
     CDS             3546..3971
                     /locus_tag="IXG44_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1658823.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            complement(4459..5160)
                     /locus_tag="IXG44_002120"
     CDS             complement(4459..5160)
                     /locus_tag="IXG44_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAQ1658824.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(5176..5616)
                     /locus_tag="IXG44_002121"
     CDS             complement(5176..5616)
                     /locus_tag="IXG44_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAQ1658825.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(5616..6191)
                     /locus_tag="IXG44_002122"
     CDS             complement(5616..6191)
                     /locus_tag="IXG44_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HAQ1658826.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            complement(7039..7518)
                     /locus_tag="IXG44_002123"
                     /pseudo
     CDS             complement(7039..7518)
                     /locus_tag="IXG44_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015510350.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
BASE COUNT         2169 a         1575 c         1213 g         2630 t
ORIGIN      
        1 tttttcattg tccataggat tgatattgtc cttgtgatga ctcattcgta ataattgagg
       61 cttgattttc ttcacttccc gttatcgttt gtccactgcc agaatctgaa ggacttgttc
      121 ctgtcgactc gtttttttga gaagagacag ttgttttctt gacttgttca tttgtctttt
      181 tccattccgc taaacgatcg gcgaccactt ttttgtctac tttttcagaa taagcggtga
      241 tcaaacgggc tactttgtcg ccatcatgag tcaaaagcaa aacggtcaaa tgcttgtctt
      301 taaattgata ttgtctgaga gaaatcgcac taagtacgtc atcgatatta ataaattgat
      361 tttcttcttt tccttttggc aaataaatgt ccaattcgcc tttttcgtaa ttggcagtta
      421 ttttatactc cgcccaccaa taagttgaga attctttttt gaaattatct actgattctt
      481 tgcttgctac gattttttct gtttctttct gagcaagatc tgctgtcatt tttcctgaaa
      541 atgctgcgga taaaataagc agtaagaaaa aaccgaataa ccaaaagaga agaaacggtc
      601 tttttttcct catgctttcc tccactccta tttaattttg actattctat tatatacaaa
      661 aaatgaaata gaaagacaaa aaagttgaaa aagatcgtaa ataattgaac ttctttttca
      721 caaaatggta agaatggaga atttcacgca tttgctctca actattcttt cttaagcctc
      781 aaatccgttt ttatctgata cgtctttgaa ccaatgaccc gattttttga ttgttctttt
      841 tccatcttgt gctaagtcta ctgaaataaa gccatatcga ttcttatatg cattggtcca
      901 cgaccagtta tccatacatg tccacatatg atagcctaaa caattggcac cttcttgaat
      961 ggcttgatgg acatatttca gatgagattg gacaaattcg atccgataat catcgtggat
     1021 catgccatct tctttgataa atcgtgcttc gttttctaca cccataccgt tttcagaaat
     1081 aaagcagtcg atatttccat aatgatctcg tacattgatc aaaatatcat agattccttg
     1141 ttcatagatc tcccatccgc gataaggatt cattttacga ccaggcattt cataattgtc
     1201 gaaataatcc tcaggcattg gaccgttttt agtatcgatt ggtgtttctt ttgcttttac
     1261 ccggcgtggc tggtaataat tgatacctaa aagatctact gtattttctt tgataaccgt
     1321 caaatcctct gcatggatca caggcatgag atcaagttct tttactatct caatcaattc
     1381 acttggaaac tcgcctttta cagctggatc aaggaatgaa cgattgaaga acgcatctgc
     1441 tatttgcgct gctttaacat cttcagggtt tgattcgtct cttggatagc ttggtgtcag
     1501 attcaagatg ataccaataa caccaccttg ctccatttca tggtaagatt tgatggctaa
     1561 cgctgaagcc aacgcttcat gatagccaac ttggatcgct tttttcatat ctgactcttc
     1621 tggataatgc catcgatata aataacctgc ttcgactgga acgattggtt cattatgagt
     1681 aaaccatttt tttactttcg aaccaaacaa ttcaaaacaa cttttggcat atttttcata
     1741 ggcttcaacc gtttctcggt tcaaccatcc gcctttttct tgcagataca tcggcatatc
     1801 gaaatgatac agattcacaa agggttcgat accgttttca atcaactcat caaaataacg
     1861 atgatagaaa cgaacggctt cttcgttgat tgttccagat tcatcaggta tcaaacggct
     1921 ccattggata gatgttcgaa atgaattatg cccagtctgt ttcatcaact gtacatcctc
     1981 ttgatacttc gtataaactt gcgaggtttt ttcaggtcct acttgatcaa agaacttttg
     2041 cggttctttt ttgtaccaat aatcaaaaat gttctctcct tttccatcgc ctgaaatcct
     2101 tccttctgtt tgtgggccac ttgctgcaga accccaccaa aaatttgctg gaaattgata
     2161 ttttttcatc aaagcacctc tttttctttt ttagttcgtt ttagttgatt ccatgtatga
     2221 tattttccat cacaatcact ttttcatttt cttcctcttc ttttttgatt cgttttaaca
     2281 gctgttctgc tgctcgttcc ccgattttat ctgttggtgg cgtgatcgtg attttcggaa
     2341 ggatactcag atcgttttta tctaaataat catagtgact gaacgcaata tccttcccga
     2401 ttctgacacc ttcttgattg aaacattcga tggcaccaat cgacatattg taattgctga
     2461 caaaaatagc agttgcccct ttttctaata atgcttttgc cccgtaatag ccgctttttc
     2521 tagaatagtc tcctgtaaag acctctaatt ggatcgatgg atcattttgg accgcttctt
     2581 tggctcctgc taaacgttct cttgctacgt agtctgtttg agtggcagca ataaatccga
     2641 tttttttatg tccttgttca ccatgatgag cgatcacttt tgctacactt tctcgatcat
     2701 tcactaatat tgtatcaata cctgctggtc cttctggaca attcaatgcg ataactggaa
     2761 tactcatttt tgtgagcatt tcaaatccag accactcttc ttcggataag aagacaatca
     2821 gcccatcgat cacatgcttc atcaggtatt caattttttg ttcaattttt tctggctgat
     2881 cggcaaaccc gcttaataat aagctatacc cattttcttc agctactctt tcgatccctg
     2941 agacaacttc cgacccaaaa cggctggaaa tcccgtttat caaaagtcca atagagaagc
     3001 ttcgattatt cttcaaccct ttagcaatga tgttttcttt atagtccaac gctgaaatcg
     3061 cttctgctat ttttttcatg ttcttttcct tcagttgctg accattcaag taacgggaaa
     3121 ctgttcccac tgaaacacca gccatcttag ccacatcacg gatcgatgcc atacattctc
     3181 actcctgcca gttacatttg atttctcttt cttgttgttc gattatgatg accgcattct
     3241 ttgcatagtg ttgcttgtca tcaatatcca tataactaaa gtcttcagta gagcaacttc
     3301 ctctcactat taattatact taaacaagta tttgaaccgg ttcattttta atatagcatt
     3361 tttttaaaag attgcaacac ttttttaaaa cgctttctga aagtggcgga ttttttgcct
     3421 ttttaaaatt ttattctaaa atttactcta tttgtgggtg ttttgtgcgt ttttctcttc
     3481 catccgtttg aagttttaca ttccaatggt ataatttctt tatcctatca aatgggggaa
     3541 gtattttgaa gaaattaatc gtactattta ttttgtctgc gggacttttg acattgactg
     3601 gctgtgattt gtttggcggc tctgaccaat cctcggacga aacaaccgat tcttctgtct
     3661 taaaagcgga aaaagcagat accaccaaca gtgtatatac gctaaaagta cgtaaagata
     3721 tggatttatc cgcgggacag ttgacattta cagataaaaa aatggaatgg acacgcacgt
     3781 atcagacaca aggaactgat tcttctgaat catctgaaga aaaaacagtg ctgacagata
     3841 tcaagatcac taccaaagat gacgaatata ttatttcagg aaaagaaaat aaaaaagaaa
     3901 caacagtcat atttacaaaa atcggtaatt accgtttgaa ggatgaggat ggcaacatct
     3961 acagtctata gtgccgtctg ttggatcttc ttttgtttct ccccttcttt tctgctatag
     4021 taaagaagca agatgattgt aaaggagacg aaatggatga tcgatagtcc tgattattgt
     4081 acatgtaata acaacaaaaa tatccataca gaatttaatg accaaggaag cttgagcata
     4141 tgcgacaact gtcacaaact agtcaaagat tcatttaaaa catttgaatc ttatcctgtt
     4201 caaaattatg taaaaagtag aagaagataa acaaatccaa gcaaaactca ctatggctga
     4261 tttttatgaa cttcaaactc agcttcttcc tttgatcaag cttcatcagt aaaacaaaca
     4321 gtggatcatt tcagtttaca aatcaaagag taaagattta tttatttcaa atatttgaat
     4381 ataaaataac caaacgatag aaaggcttat tctacatcac gtagtaattt ttctttcccg
     4441 tcgtttggtt ttttattttt agcctaattc ttgacctttt gcatgctggt tatccgtaaa
     4501 atattttcca gcaggctttc cttccaattc agctaatttt tcttggatct gacggatcac
     4561 cttttttgca tcttctggat tttctacaaa atcgaattta tccccatcca cttgtatttt
     4621 aggacataca tcaaaatctt cataccattg attatagcgg gtgttcaacg tttcataata
     4681 ggtatataac gtctcatcgc tttctagctg ttcatagtca cgcccacgtt ttttgatacg
     4741 ttctagcatc gtgtccaaag aaactttgat atggaccatc aaatcaggcc gtttttttgg
     4801 agcaacatca tctaactcgt ttagcatcgt atctaataag ctatcgtact gctgtacttc
     4861 gatatccgtt acccgaccta aatcagcgtt tagatggaac aacaggctat cttcatagat
     4921 ggatcggtct agcacattgt catcgttcac taaagcattt ttcattgcta aaaatcgctt
     4981 gttcaaaaag aagatttgca ataaaaaagc gtactgctca ggatttgaat aaaagagcgg
     5041 caatacttcg ttatcttcga cattttcata aaatggttta gaatcaatct cttttgcaag
     5101 catatccgtc aaactgctct tacctgcacc aatcgttcct gaaatcaaaa tcactgacat
     5161 acaacccttc ctcttctata agtactcgcc tacgtaagtt tcaacaggca atgtttcaaa
     5221 atcatattca cgtacagctt ggtctgattt taaatacgta tggagacttt gtgtaatcat
     5281 caagttcgta ggtaccgtct cttcttgtac aatgatcatc ggttttttca acatagttgc
     5341 cacacctaat tcgtaagccg ttcctggatc gattgtttga tgctcaaagt caaggatagc
     5401 aacaacaaat tctgcatttg tcagttcttc catatctttt tcgtatactt cctgcgccca
     5461 tccagcgctg aatagttcat aattgctttc ttggtgatga cgtggagagt agaaacttgt
     5521 gactgttttg ttttcctcca acgctttttc gatacgactt actcgatcga tttgctcctc
     5581 tgaaaaaaat ggtcctgcta aatatatttg actcattatt ttgcctcctt caataataac
     5641 tgatttttaa tagatactgc tcgtaattca ttgaccagtt ccaatggatt gttggaaaag
     5701 attacttgat ccaaccattt tttatccata aaatcctctt ttgcaaaagt ttctaataat
     5761 tgttgcaagc cgctgtaata accgccaata ttcaatattc cgattggctt gtcgggcgtc
     5821 agaccgattg ctatccaaga aagaagttgt gagaattcct ccaatgtacc tagacctcca
     5881 gggaaaacga ggacagcatc tcccttttca aataataata cctttctttc atcgatcgtt
     5941 tctgtaggaa taaatgtagt aacatctttt ccaggcaatt cagtttcaaa aagcccattc
     6001 ggatagatac ctgtgacttt tccgttattt tccagtactg ttttactaat cactcccata
     6061 atcccggagt atgaaccacc gtaaaccagt tcgatttctt cttgtgccat gtattttcct
     6121 aacgtttgcg caataaattt gtaactttct ttattaccaa aacgggaacc gcaaaaaaca
     6181 gtaagtttca taaaaaaaca ctccctaact caaaagtcta tcttcattct acacgtgttt
     6241 ttttaaaata aaagactaga ttttgtagtt ttttagtggt atataggata gacgccacta
     6301 tatatagtat ttagaaaaaa atcatttttc agccatttta tagataaaac aaaaataaat
     6361 aacgtgtttt catcgatatg aaatgactta cttatagaag atactgggat ttcgtcctat
     6421 atgccaataa cgtgtattat tttaaacaac aaatacttgt ttcttttatt aacgaaaaaa
     6481 tcattttgtt ctcgtgttat gtttcactga taaatgatgt ttctctaaat aaaaaagccg
     6541 acgaactaaa cgatcaaaat ttgatcattt ttgttcatca gcttctccta aaaatctttt
     6601 tgttaacgat aaatagctgg atttgtaaaa atatcacttg aatcgtcact gctggaagaa
     6661 aattcagtga taaccgcacc tgttgcgcct gcctggaact agtgtttgat attgggctcg
     6721 aacgtgtact gatctctagc atgaagaata atttcttttt ttactgtata ataactttct
     6781 ttcctttttg gaatagtaca ggaaagtttt tttgtttcct ttccttcgac ataaggtata
     6841 ccagaccctt tcgacaacga aaggttctca tttttccttc atcgtcattt caaggagggt
     6901 gccgatgctc tggacagttt cgatcgctcc taaaccaaaa tctgtatatt cgattctttc
     6961 taactctgat tctttcatac cattcctgtc tcaaggtaca tctattgtac ttttttcttg
     7021 taatccattc tgcctctcct aactaattgt caaatcatcc caagatatgt cttcttcctc
     7081 tttttcttct tggatgattt ctgcattcgg atctactggt tttttcaata ataaaatggt
     7141 tattcctgtg acaaaagaac cagctaacaa tgcaccaatg aatgcccatg gtctggtcat
     7201 cgtagcgact gcaaacattc ccgatgccgg aatacggcta tctgcgcccc aaaacatgct
     7261 taatccgcca cctactgtcc caccgactcc cgttgctatg acattgcgtg ccaagtcgtt
     7321 caatacgata gggaaacatc cctctgtgat ctgacagatc cccataggaa aagcggtctt
     7381 caatgtatcg atttcttgac gattgagtat atttttacga atgattttcc ctaagaaata
     7441 agcgatcgtc aagccgaatg gggttaccat cgaagctaaa atcaatacag tgattggttc
     7501 atagattcct tctgaaaatc gtgccacacc cccttatcca gaataaatgg taatccagaa
     7561 acagccgtgt atctgcggta ataagcc
//
LOCUS       DACEUZ010000110         7555 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.110, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000110 DACEUZ010000000
VERSION     DACEUZ010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7555
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.110"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(102..626)
                     /locus_tag="IXG44_002124"
     CDS             complement(102..626)
                     /locus_tag="IXG44_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1658827.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(619..1092)
                     /gene="tsaE"
                     /locus_tag="IXG44_002125"
     CDS             complement(619..1092)
                     /gene="tsaE"
                     /locus_tag="IXG44_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HAQ1658828.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(1215..2195)
                     /gene="pta"
                     /locus_tag="IXG44_002126"
     CDS             complement(1215..2195)
                     /gene="pta"
                     /locus_tag="IXG44_002126"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HAQ1658829.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(2348..3028)
                     /locus_tag="IXG44_002127"
     CDS             complement(2348..3028)
                     /locus_tag="IXG44_002127"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAQ1658830.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            3154..4023
                     /locus_tag="IXG44_002128"
     CDS             3154..4023
                     /locus_tag="IXG44_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ1658831.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            4049..4564
                     /locus_tag="IXG44_002129"
     CDS             4049..4564
                     /locus_tag="IXG44_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1658832.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(4611..5174)
                     /locus_tag="IXG44_002130"
     CDS             complement(4611..5174)
                     /locus_tag="IXG44_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1658833.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(5229..5408)
                     /locus_tag="IXG44_002131"
     CDS             complement(5229..5408)
                     /locus_tag="IXG44_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1658834.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(5485..5994)
                     /locus_tag="IXG44_002132"
     CDS             complement(5485..5994)
                     /locus_tag="IXG44_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1658835.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(6090..6470)
                     /locus_tag="IXG44_002133"
     CDS             complement(6090..6470)
                     /locus_tag="IXG44_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAQ1658836.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            6800..7549
                     /locus_tag="IXG44_002134"
     CDS             6800..7549
                     /locus_tag="IXG44_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HAQ1658837.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
BASE COUNT         2144 a         1567 c         1339 g         2505 t
ORIGIN      
        1 tcaaaaaaaa tggactgcga cacgactttt gtcacagtcc cttcgtgtgt ttttctgcct
       61 tctcaaaagg atgacagttt ctcactcttt tcctatttct tctattcaat caaataactc
      121 attttatata catctaaaaa ttcacctaga tctgttcttg cccctctagg catgaccgct
      181 tcgatttgga agcccatttt tcggtaaagg tgtacggctc tttcattacg aacttgaacg
      241 tcaagctcca atcgaaaaag tacacccatt tccttcgccc aactgatgat ttcttctagc
      301 ataagagtgc ctagtcccat tccccaatat tcctgcagaa tactgatacc gacttctccc
      361 acgtgagaca aacgaaattg actgtctgcc ttgacagatg ctgttccaat aatcgtccct
      421 tcatagattg caagcagcaa taaattatta ttgctttcgt atatataatc caactcttct
      481 tctaaggttt ctggtggtaa caggagttct gcttcatcca aaactaaaaa ttctgtctcc
      541 ttattaactt gtcccatcat caaaagaatg tcttttgcat ctcctggcac tgcttcccgc
      601 aagatgatcc caacttcttc actcatgatt ttcagcccat ttttctagaa ttcgctgttt
      661 gaaggtttca ctttgctccc cgtatgagat tagtttcaca taccgctttt ctaaatctgt
      721 atcacttttt tctaaaatca gttccaaata atcctccggc agtgcttctt ctaatagatt
      781 tccccattca acaacggaaa gcccatcacc ttcaaaatat tcatctaacc ctaaatctgc
      841 tccgctagct gctacccgat aaatatccat atgatacaac ggaatccgac cttgactata
      901 ttctctaata atcgtgtatg tcggactttt gatcatttgc gttatcccta atccttgggc
      961 aattcctttc gtcaacgttg ttttacccgc acctagatca ccagtcaata tcagattgtc
     1021 acctggcatt gcggaatgac caattgcctc tgcgaattgt tctgtcattg ttaatcctga
     1081 tagttcgatc atttttcatc ttctcccgct cttctttctt tttgccagta tatcgcgtaa
     1141 tgaagttttt ttaaagaaaa aatgaactag tcaagaacag cagctgctgt ttttggctag
     1201 ttcgcgtgta tttgttaatc aatcaatgtt tgtgctgctg tgataatcga taatttatat
     1261 acatcttctt catttgctcc acgagacaaa tcagaaaccg gtttgttcaa gccttgtaga
     1321 attggtccga ttgcttcaaa gttaccgaaa cgctgagcaa ttttatagcc gatatttcct
     1381 gactgcaatt ctggaaatac aaagactgtt gcttgaccag ctacttctga atctggcgct
     1441 ttcagttgtg caactgaagg aacataagaa gcatcaaatt gcaattcgcc gtcgatcatg
     1501 tattgtggcg ctagtttttt agcgatttta gttgcttcaa ctactttgtc tacttctggc
     1561 gatttcgctg agccttttgt cgagaagctc atcattgcta cttttggttc gatatcgaac
     1621 aattctgctg tttttgcaga atctacggcg atttcagcca attcttgcgc agttggattc
     1681 acgttgatcg cacaatccga aaagacatat ttttcttgat ctcttccacg taccatgatc
     1741 atcgctccgc ttgtccgact gatgccaggc tttgttttga tgatctgaag tgctgggcga
     1801 acagtgtcgc cagtcgaatg gatcgctcca gagaccattc cgtctgcaat ccccatatac
     1861 gtaagcatcg taccaaagta attcacatct tttaaaatct tttctgcttg ttctttggat
     1921 gcctttccat ttctacgttc aacaaaagta tccaccattt catcccactt atcgtaatta
     1981 tcagggctga tgatcgtaaa gctagatgtt ttgattcctc tggcatgcgc tgcatttctt
     2041 atttcttctt cgttccctac taaaatgggt tccacaagtt cttctgcttt caaccgggca
     2101 gctgcgccta aaatacgagc atcagtagct tccgggaaaa cgattttgat cccacggcga
     2161 acaattttaa atcttaaact atcaaacaat tccacaagat gacctccaga cgtttttata
     2221 tgtattatca tacactattc cagaaaaaaa gaacagttta ttttatctgt atcagtcaaa
     2281 aatgctggaa aaattaagtt tttcacatac aaagcgaact gcactataac aggaaagcgc
     2341 tgttttatta cactgtttca ggcagttgcc aatcaatcgg ttcttctcct aacgcaagga
     2401 gcgcatcatt cgttttcgag aagggtttgg aaccaaaaaa gccacgactt gcagataacg
     2461 gactgggatg tggagatgtt atgatgatat ggcgactctt atcgatcatt ttgatttttt
     2521 cttgtgccgg ttttccccac aagataaaca cgattggttt ttcccgttca tttaattttt
     2581 caatgatcgt gtctgttaat ctttcccaac ctttcccgcg atgagaatac gcttgccctt
     2641 cccgaacggt aagaacggtg ttgagtaaaa ggactccctg tttagcccat gacactagat
     2701 ttccatgttg aacaggtgcg attccaagat catcataaag ctctttatag atatttctta
     2761 acgatggcgg gatctttacg ccaggttgca cagaaaaaga taagccatga gcttgatttt
     2821 ctccatgata aggatcttgt cctaaaatca ccactttaac ttcttcataa ggagtcaatt
     2881 ccaatgcttc atagatatga tacatatctg gatggatctt ttgggacgca tattcttttt
     2941 ttaaaaattc tcttagatgc agataataat cttttgaaaa ttctgaggac aaaacttctt
     3001 gccaactgtt atgaatgatt tctttcaaaa actccacctc ctaataaaaa gaataactca
     3061 cattttcttc aatcacaagg gacaatcttc accctcagca cttgattaat atggtaaact
     3121 aggaacaaga agcagaaagg aacgataatc attatgatca aattaattgc atcagatatg
     3181 gacggcacat tattagatgc ccatatgagt gtttctcctg aaaatgccga ggcgatacgt
     3241 tttgcaaatg atgccggtgt tgagttcatg gtcgctactg gtcgaaacta tcaagaagct
     3301 cgcgcagctt tagatgaggt aggaatcgat tgtgcgatga tcactttgaa cggggcacag
     3361 gtatttgata aagaaggaaa ttcattgttt accgtgtcga ttccaaataa tcaagcaatc
     3421 tctgttttag atattctaga tgctaacggt atttactatg aagtagcaac caacgatggt
     3481 ctttattctg aaaatcaagc gaaacgaatt gaaagttttg cttccatggt ggctacccat
     3541 ttacctcacc ttacgtataa aatggcgatt gccatggcct cagctaagct ggagttattg
     3601 cacatcacat acgtggacag tattcgtgct attttggaag aaaagaatct ggaagtttta
     3661 aaaattattt gttttcatac agaagggcca gctgtattag gtccagttgg aaagcagatt
     3721 tctcttttag gcgatctagc cgttacttcc tctggtcaaa acaatatcga agtgaaccat
     3781 aaaaacgctc aaaaaggaat tgctgttgcc catgtcgctc acgagcgtgg gatttcttta
     3841 gaagaagtca tgacgattgg cgataacttc aatgatgtca gcatgctgca agtagcagga
     3901 gtcagtttcg caatgggaaa tgcagaaatc gaagtaaaag attatgccaa atatacaacg
     3961 gacacaaacc ttgaatctgg cgtaggaaaa gccatattgc gcgcgattgc agaagacttg
     4021 tagttttatc tagaaaagag ggatatagat gtcagaattt ttcattcagg aaaagcagct
     4081 tggacgtgca actcggacaa ttgtcaaaaa cgaaaaagga cattccctgt ttttgatggt
     4141 tggtcgctgg ggaacaaagg gcgatgcatt gtctctttat gccatgaatg gcgatctcgt
     4201 tgctcacatc aagcagataa gcctgacatt cggtacgcgt tttgagctgt ataaagagtt
     4261 cgaaaaggtg ggaaccatgc agaaaatatt caattggccc ggtgactttt attatattcg
     4321 tagacttcac tggacagtcc aaggtgatat ctataatcat caatatcaaa tccatcattt
     4381 caatcagcaa atcatgaaaa tggataaagc aacacttttt tcaggagatt actatgtatt
     4441 ggatgtctca aatgatgaag atgctccagt ttgtatttgt attgctgcgg ttttagatta
     4501 ctggctctac cgtaaagaca aaagtcgcga taagcctgtt tcttaccgac ttaacttaag
     4561 ttaaaaagat gtacctgaaa tgcggaaata cgcatttcag gtacatcttt ttagatattc
     4621 ttttgcatat gatcataaag atgaccactg atcatccgct gaccgacaac tttgaagtca
     4681 tggcgttcgt ataatttttt agcctttgga tttttttcat ccacacttag accgatgacg
     4741 gagcgattag ctttcttcgc caatttaggt aatgcttcta ataatcgcga accgatgcct
     4801 tgtcctctaa aatcttctga tacactgata gagtccaaat accattcgtt gggaaacgtt
     4861 tctttatcaa tgaacaaacg tgtagacgca tctagctgat atttctctaa gataggtacc
     4921 agtggctgat cgatgattga ttcttcttca tctgtataac cgtaagcaac tcctgcaact
     4981 ttaccgtcta tttcttctac gatacctcgt gaataactgt aacgataatt tggatcagtg
     5041 attgcttctt ctaaaatctc taaagtcttt tgttcccctg tctgcttcaa aaatggcagt
     5101 tccatatctt ttagaatcac taagatcaat ggggcgattt ctggagcatc ttcttttctc
     5161 gcaaaacgaa tcatagtttc ctctcctttt ctccattcta acatgcagaa aaagaaatcg
     5221 caaaatcgtt attttaaaaa atcttcaatc ttttcagtaa tgaaataacc atgagtaacg
     5281 attcttgccg cttcagttcg gcaataaggg cagacgactg gcatttcttt atcattatgt
     5341 tcgactacat tattgacttc ttcgtatgca aatttttttc cgcaattagg gcatttcatt
     5401 ttttccatgt tatctcaact tcctttcatc tttactctct tagcttactc ttatattgta
     5461 aaaaagagaa gcaatatcct tcttctatgc agatatccat acctgacggt cctgttctcc
     5521 atctattaat cggactttat aataaggacg ttcatctttg actagaagtg tccactcttt
     5581 cacttttgct tttttcccgt agtagctgac tgcttccttc atcgctgctt gcggttgttt
     5641 gaactcttcc gttttttcta tatcaaaaag atgttctgcg gccttttccg tgtcatttcg
     5701 tacggttgtt ttcccagtgg atggattgac tgctatgact tgtccttcac aaaaaaactg
     5761 gtattcatat gttgcattcg taggattctc caaatctttt tcaactgcaa aatgaatagc
     5821 agatatgtta tgttcttttg tttcgttttt tacgattgct gctgcttttt tcaatgagat
     5881 ccgatattcc tggtcactaa tatcatccat gtgaaaagcg gcattgagca gtcccgtcat
     5941 accggtaatc cctattacga tgagggcagc aacaccaaac atcatttttt tcattcttca
     6001 ttctcctgtt tctttttttt aaagaatatc aagaaatgaa cgaatgaaaa atcgaatgaa
     6061 acgaaaaaaa agtgacaatt ttgttatctt tattcgtgca gttctaatgg caatccgtct
     6121 ggatcaaaga aaaacgtcat tttttttcct gtaaaggtat cttctctaat cggttctgat
     6181 tcgatcccta aggactgcaa ttctgcaatt gtttcttcaa tatcgtctac tttgaaagca
     6241 agatgacgca agccacaagc ttctggatag tttggtcgtt gtggcgcatt agcaattcca
     6301 aaaatctcca attcagcact tcctagtttc agatctaatt tatagtcatc tcgttctttg
     6361 cggtaatttt ctctgattac ttcaaatcct aatttattaa cataaaaatc acgtgatttc
     6421 tgataatctg aaacaatgat ggctatatga tggatcgttg taaggttcat atgagcactc
     6481 ctattcttcg tttaagctcg gatctatatt aatctccagt tccattgtaa atgtgtcaga
     6541 ttgaaaaaaa tagctgtttt tcctttttat aagaaaaaag gcaaaaaaaa cagaaagtag
     6601 gtcctccaag acactcttcc tgttcccaat ataataaatg aaagtaagaa attatatttt
     6661 caagaaaaca aacagaaaat tatcataaca caaattattt ataattgaaa gactagtttt
     6721 ttttcaagaa aagcatacaa tagattgtgt tagtaaatac agaacacact acatttagtg
     6781 tcttaaggag tcctcataca tgaagtcata ttttcagttt ttagctcatc agctaaaagg
     6841 ctggccgcaa caaaattatt atcttttctt tttcagtctt ggctgccagg tcatgactct
     6901 tgtcaatagt cccatcactt ggctgtccgt tattacattt atcggtacca cgcttggtgt
     6961 attgtgtatt ttatccatca atgcggcaaa atcagtcaac ggtattttag gtattttgtc
     7021 tgcgatttgt tttattatcg ttgggttttc agcaaagaac tatttaagta tcggtgaaca
     7081 gattgcttat atgatcacat tggatatccc cgtattgtta agtaaagaat ggaatcataa
     7141 tatggcttca aaaatccgaa aattcactgg aaaaagctgg tttatcgcaa tcatcagtac
     7201 tttccttgtc tatcttgttt caggattcct gattggtcgt tatacagatg atcctcggcc
     7261 gtgggtagat gccattgctt tttctattag cttgactgct ggtatcatca gtttcttgcg
     7321 ttacaacaac caatattact ggtggttagc ttcagggctt gctcaaatgg tcctttggta
     7381 tatttcattt acgcatggat ctgcaagttt ggcgatgttc gtgaacagct ctgtttactt
     7441 gctgaatgat gtccttgcat ttaccgtttc accttggtat aaccaaaaag aacgtgaacg
     7501 tatgcaaaaa atggaagaag aatactacaa cgcacaacaa cacgcataaa aaaac
//
LOCUS       DACEUZ010000111         7550 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.111, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000111 DACEUZ010000000
VERSION     DACEUZ010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7550
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.111"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(93..1196)
                     /locus_tag="IXG44_002135"
     CDS             complement(93..1196)
                     /locus_tag="IXG44_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAQ1658838.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            complement(1286..2221)
                     /locus_tag="IXG44_002136"
     CDS             complement(1286..2221)
                     /locus_tag="IXG44_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HAQ1658839.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            2523..4457
                     /locus_tag="IXG44_002137"
     CDS             2523..4457
                     /locus_tag="IXG44_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ1658840.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            4914..5330
                     /locus_tag="IXG44_002138"
     CDS             4914..5330
                     /locus_tag="IXG44_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HAQ1658841.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            5344..5910
                     /locus_tag="IXG44_002139"
     CDS             5344..5910
                     /locus_tag="IXG44_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HAQ1658842.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            5933..6919
                     /locus_tag="IXG44_002140"
     CDS             5933..6919
                     /locus_tag="IXG44_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAQ1658843.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(7074..>7550)
                     /gene="scm"
                     /locus_tag="IXG44_002141"
     CDS             complement(7074..>7550)
                     /gene="scm"
                     /locus_tag="IXG44_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAQ1658844.1"
                     /translation="STDTSASSDSTDTSTTTSDSTDTSASSDSTDTTSTTSDSSDSST
                     TTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNR
                     TNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
BASE COUNT         2148 a         1452 c         1562 g         2388 t
ORIGIN      
        1 aataccccta ccgcaaaagg aaatacacta gccacctttg ttcgtatttc aactacttct
       61 aaaaagattt ttagtgtcat ggttcgtact ccttatttta gttcagaacg gtcacccatc
      121 gtgtaatggg agtcctccga taatttgaag gtgtctttga ttggatcggt cacatatact
      181 ttatcgtctt ttgtcacgaa gatcgcttcc gtcccgttat tcaattcgct ttcgacgtat
      241 tccagtcctt ttttcacacc cattgaaaag actgctgtag acaacccgtc accatcaatt
      301 gatttatctg ttatgatcgt gacacttgcg atatcattgt cgaacggata acccgttttt
      361 ggatcaaaca aatgatggta ttttttgcca tctacttcta aataacgctc gtaaattcca
      421 gaggtaacta atgttttgtt ccgttcttta atagaaccta aaacagatcc gcgagccatg
      481 ttcggatctt ggatgccgac tgtccagtct tggttttcgc ctcttgggct gtggcctaag
      541 acatagacat taccgcccaa gtcaacgatg gcagtggtca ctccttgttt tttcaagact
      601 ttcactactt cgtccgtaat gtaacctttg gcgattgcac ctaaatcgat gatcattcct
      661 ttttctttta gataaacagt tttttctttg tcatttagtt cgatcttatg ataatccact
      721 aatttcaatg cctgatcgat ttcttcttgg gaaggttttc tggcgtcatc aaaaccgatt
      781 cgccataatt gtgtgattgc accaatcgcc atatcgaatc cgccttgaga atcttggcta
      841 tattcgtagg cccgcttgac taacgtgtag acgtcatcag aaacttttac tggtttgatc
      901 ccagcttgct cattcacttc atcgatctca gaaccttttt gattgatcgt gattttatca
      961 ccaagttctt ttactcgatc gaaggcaggt ttcaatgctg attctttgcc ttcatcatac
     1021 acacggatac gaacgtaagt ccctaacaag aactgttcat cagaatatgg atctttcaat
     1081 atcttggtag ctgctgaact ttcagtggtt gttttatttg ctgagttgct gcagccagtt
     1141 agcaccataa aagagatgcc cagtgtgatt aaggaaaata gtagtttttt ccgcatcttt
     1201 ttcctctctt cattttgaaa ggtgagacga ctgcctaatc tggctatcgt cccaccctcc
     1261 aacttttatt agtatgatta tgtgattatt tttctgttac gatattatct acttcgattg
     1321 tttgagtgtc gcctttttca gctgcattca ctaattgttg tgcgtacatg atgaatgaat
     1381 gtgagctgtg tgtagcacct gtaacaactt ctacattagc aggtgagcca tcttcttcgc
     1441 ccattgcttt gacgaattcg tcgttcagag tagggatgta agtttttggt tctgtaccgc
     1501 tttttgcttt catattttca ttatattctg tatcatcttg ttttgattta ccgtctttgt
     1561 cgatataatc aaagtttgat tcagttactt tgccgccagc aactgtcatt tcgaattgca
     1621 cacggtagcc gttagagtag ttcttttctt caagcttgta agtgccgtct ttcaaatcag
     1681 caccattgtc gatttcgatt gtatctgtgt taccagcttg agcagcttgg atcaattgtt
     1741 gtgcgtagtt ttggaagctt tcagatgagt gagtagctcc agttacgact tccacaccgc
     1801 ttgcgctttg tgcttttaag aattggtcat tcaactcagg aatgtatttt tctgggtttg
     1861 tgccagattt tgcttccatg tttttgttgt attctgcatc ttttgttttt gattcgccgt
     1921 tttcattgac gttatcatat tttgattcag tgattttgcc gtcttttaca accatttcaa
     1981 agactgcacg gtaaccattt gaataatttt tttcttctaa tttatatgtg ccgtcttgca
     2041 aatctccgcc agctacaact tttgcagatg attctgttgt gctttttgca gtcgaagatt
     2101 gtgctgtaga tgattcagaa gaagagtttg agcttccgtt gtcatctgcg ccgcaagcgc
     2161 ctaacactag agctgaaaat gctaatacgg caaatccagt tacaaaacgt tttttcttca
     2221 ttttaatacc ccaccattcc atttctagtc cacaattttt ctttgtgatt cattatacat
     2281 tctaatcttt ttagcctata agtcaactat ttttgataaa taacccatgt aatatttcaa
     2341 acgttttctt tcagcaaatt tcgtgaaaaa acgctttatt ttaaaatttg aaaaaaatcc
     2401 aaaaggagtt agacaaaaga acagaacatc tatataatat atggagattg tgtaataagt
     2461 atcaaactaa aagaattatc atatattcat tatatagata gtctataagg agcggatgat
     2521 caatggcaaa gcaaaatatt gtcgttgtag gagcaggata tgccggtgtt tcagctacaa
     2581 aatacttggc aaagaaactg aaaaaagaag atgtagcaat tactctgatc gatcgtcatt
     2641 cgtaccatac tatgatgacc gagttgcatg aagtagctgg cggacgtgta gaaccagaag
     2701 caatccaata cgatctgcag catttgtttg ctcgtcaaaa gaatgtacaa cttgtcacag
     2761 acactgtaat cggtatcgat aaagaacgaa aaatcgtcaa gaccaaatta ggttcctatc
     2821 cttttgatca gctgatcctt ggaatgggcg gagagccgaa tgactttggt acacctggtg
     2881 tcaaagcaca cggattcacc ctttggtcat ttgaagattc tgtaaggatt cgggaacata
     2941 ttgagaagac agtagcaaaa gctgcattgg aaccagatga tgaactgcgt aaagccatgc
     3001 tgactttcgt tgtctgtggt tctggattta ccggtattga aatgattggg gaactgatcg
     3061 actggaaaaa tcgcctagcc aaagattata aattagatcc aaacgaaatc acactgatgg
     3121 tcgtagaggc aatgccgacg atcttgaata tgttggatcg aaatgatgct gcaaaagctg
     3181 aacgctattt gcgtaagcac aacgtggaat tattattgaa tgcaccaatc gtagaagtgg
     3241 ctgctgatca tatcaaattg aaagacggac gcaacattcc gactcacaca ctgatttgga
     3301 cagctggtgt caaaggaact tctgacgctg ctgactttgg attagaatca gcacgcggac
     3361 agcggttgat cgccaatgaa tatatgcagg caaaaggcta cgaagataaa aacatctata
     3421 tcattggtga tttagtcttt tatgaagaat tcccaggaac accgacaccg cagatcgttc
     3481 aagctgctga acaaacagga cacacagccg cagcgaatat cgtggcttcg atcaaaggcg
     3541 gagaaaagca taaatttaaa ggaaactatc aaggatttat ggtttctatt ggtgccaaat
     3601 ggggggtagc taacctattt gataagatcc atttaagcgg atttttagct atcgtaatga
     3661 aacatatcgt gaatttgaaa tacttctttg atattcgttc tggttattat atgttccaat
     3721 atttaatgca tgagtttttc catatcaaag atgatcgcaa cgtgactcga ggacattctt
     3781 ctcgttacgg caatgtctta tggagtgtgc cacttcgtat tttctatggt ttagtttggt
     3841 tcgttgaagc ttccaaaaaa atcattggga atggtgaagt gttcaaacca agtacttggt
     3901 ttggtgaagg atcatggttc actggaaaca gtgtgttccc ttggacatgg gaatacaatg
     3961 taacagatgt tgccagtggt gcttctgctg ctggtgatgc aaccacagct gccagcggag
     4021 caggggcaag tgccggagaa gcagcgactc aagctgcaca ttttggtttg agttatgcat
     4081 acggtgaaga accaatggct gtgattgaga agtcaccaga ctggttcaca aagatcatgc
     4141 aagtgatcat gccaaacaat gatgtagcaa tgttcttcca aaaattcatg gtattctttg
     4201 aaattgcctt ggcattagca ttgattgctg gattattcac atggctatgc agtgcgacaa
     4261 cgattgtatt ggtgatcacg ttctgcttat caggcatgtt ttactgggta aacatttggt
     4321 tcatttttgt tgctttcgca ttgatgaacg gttccggacg tgcgattggg ttggatcgct
     4381 gggtaattcc atggctacaa cgtacactag gtaattggtg gtacggtaga ccaagttccc
     4441 agtacggcaa gaaataaata gataaagaac gttatttgaa tgcaagattt caatagaaag
     4501 gggctgagac aaaccgtcac agcctccttt ttatgtaaaa ttaaaggaat aagatgcccg
     4561 agataagcct ccatgagcaa gacacaggtg tcacaacctc tgttacaacc tcttgttata
     4621 aacggtgttc cagaagtagc ttcttcgaaa aataagccga aatttcacaa aaacttgaaa
     4681 agtaattttc gtaaattcct tcttatttct tgaagttgcc cacttctgtc acaacctctt
     4741 gttataaacg gtgttccacc tgctgcttct cggtaataag cccaaagaat tagaaaacat
     4801 ggaaagctgt tttctattct tcgttcttat tactcaaagc aagacacagg tgtcacaacc
     4861 tcttgttttt atgctaaaat tgggtgagtt aatctggaaa aaggagaaca aaaatgagcc
     4921 ttaaagaatt tgctgaaaaa agtctgctca aaccttggga tgtttttatt attttgcttt
     4981 tgactgttct ttctttttta ccagtcatga tttttggtct tcagcaaaac accgcaacag
     5041 cagaaaaaga agcggtactg cgtgtggatg gcacagaaat caaaacattc cctttgatgg
     5101 ctggagaaga gagcaacaca tatacttaca aagatgaaca cggagattat aacttgatcg
     5161 agatagatgg cgatcgagta cggataaaag aagcagattg cgatgaccag atctgcgtac
     5221 gtcgaggctg ggcaacgaaa aatggcgaaa ccatcgtctg tttgccacat aaattagtga
     5281 tcgaagtccg ttcaacagat ggaggtgacg aagacagtct gatttattag accttcacac
     5341 accatgagta aattacaaaa aatgatttat atttcgctac ttgttgcaca aggagtgatc
     5401 attggtttat tggaaaatat gatcccttat cctttttctt ttgcgcctgg tgcaaaacta
     5461 ggcttagcca atctaatcac gatcgttgcc atttttacca tgaaaaaaca agacagcttc
     5521 ttactacttt ggctacgact attcctgact actttgctag gcggaacgat ttccacattc
     5581 ctttatagta tgagtggtgc tttgcttagt tattttggaa tgttattggt caagcaatta
     5641 ggaccaaaac gtgtcagtat catcggaatt agtgcggccg gcggtttcat gcataatgtc
     5701 ggacagcttt tgatggcttc tttcattgca caatcatgga cagtcatgtt gtacttgcca
     5761 gtcctttcag ttttagggat tttatcaggg attgcaatcg ggatcgcagc gaattatctt
     5821 ttacgacatg tgcagacact tcaacgattc caaaaagatt atgagcaggg aaccaaacaa
     5881 aaatggagcc caatgtttac caaaaaataa tcgtcacaca aggagttttt ttatgaagct
     5941 acatccaatg tgggaaaact atcctgcttt aagtaaagaa ttgacagaaa cactaaaacg
     6001 gatggaagaa actgtccgcc tgaaaaacaa acctgtcgaa caagcaatca aagaaacgat
     6061 ccatgccggc ggaaagctgt tgcgtcctgc ctatcagcta ttgttctcac aatttggacc
     6121 agagcaagat cgagacaaag ccatcgcttt ggcagcttct atcgaaatgc tccatacggc
     6181 cacactgatc catgatgata tcgttgatga agcggatctg cgacgtggtc agcctaatat
     6241 ccgtagtcag tttggtaatg tcgtttctgt ctatgcagga gactatttat ttgtctgctg
     6301 ttttaaacta ttgtctgatt actccacttc cttgaaaagt ctccaattga attctcgcag
     6361 tatggaaaaa gtattgaatg gcgaattagg gcagatggat gatcgctata attatgaact
     6421 ttctgtaaaa gaatatttgg aaaatatttc aggtaagaca gctgaactgt tccaattaag
     6481 ttgttctgtt ggcgcttttg aaagtggaac aagcgagcgc ttcgcaaaaa aagcaggaga
     6541 tatcggactt tctatcggga tggcctttca gattatcgat gatattttag attatacaaa
     6601 agagagccag gaaatcggca aacctgtact agaagacatg cgtcaagggg tctattcttt
     6661 accgttgatc tactctttgc aaaaaaacaa accaaagcta ctaccttatt tagagaagaa
     6721 agcggcaatg acagaagaag atgtcgacgc cgtacgcaaa atcgttgaac atacaggagg
     6781 cgtggaagaa gctaggaagt tagcggcctc ttacacagaa aaggctttga aggaaataaa
     6841 gaaattacct gccacctctc ttcgaacaaa agagaatctc ttttccctta cccaactgat
     6901 tttggatcgc aaagactgaa tagggaaagg ttcgatacgt aaataaattt ctcagaagga
     6961 aatctacgtg ttttttgaca aattgaattg tagttcttgt atttatgata aaaagcaaaa
     7021 ataaagagca acgtccgtta gcgacgttgc tcttttttat cttgaatatt tttttacctt
     7081 ttcgatttct tataactcat aaaagtgatt cttaaaccga ttacaagtaa tataacgcct
     7141 gcgagtgtta tccaattatt cgattgactt ccagttttag gaagtgctct ttttgatttg
     7201 tttccattgt cattgcttgt tttattagtt aagttaaagt tcgtcctgtt atccgaactt
     7261 gtttttgaga aggagacact tgcatgacgt gtatcgcttg tggtagacga cgtgtctgta
     7321 ctatcgcttg aagttgatga atctgagcta tcacttgtgg tagaggatgt atctgtactg
     7381 tcacttgaag ctgacgtgtc cgtgctatca cttgtagtgg tagatgaatc tgagctatca
     7441 cttgtggtag aggtagtatc tgtactgtca cttgaagctg acgtgtccgt gctatcactt
     7501 gttgtggtag atgtatccgt actgtcgctt gaagctgacg tgtccgtgct
//
LOCUS       DACEUZ010000112         7511 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.112, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000112 DACEUZ010000000
VERSION     DACEUZ010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7511
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.112"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(2..2167)
                     /locus_tag="IXG44_002142"
     CDS             complement(2..2167)
                     /locus_tag="IXG44_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1658845.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            2427..4721
                     /locus_tag="IXG44_002143"
     CDS             2427..4721
                     /locus_tag="IXG44_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAQ1658846.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            4714..5379
                     /gene="pgmB"
                     /locus_tag="IXG44_002144"
     CDS             4714..5379
                     /gene="pgmB"
                     /locus_tag="IXG44_002144"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAQ1658847.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            5372..6379
                     /locus_tag="IXG44_002145"
     CDS             5372..6379
                     /locus_tag="IXG44_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAQ1658848.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            6401..7420
                     /locus_tag="IXG44_002146"
     CDS             6401..7420
                     /locus_tag="IXG44_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1658849.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
BASE COUNT         2340 a         1638 c         1410 g         2123 t
ORIGIN      
        1 cttacatttt aatggagccg atcactttac cagctgtcac tgtacctaat tgcttgatca
       61 ctacttcttc tactttatcg tttgtaaata cgatcatgat cgttggatct ttttcttcgt
      121 tttggatcac atcaagatcc atctcagcta atttcgttcc tgttcctact tcttggcctt
      181 ctttgacagc aatctcaaaa gctggctgat tcatttggac tgtatcgatt cccatatgga
      241 tcaaaacttc gataccatct ttcgtttcca gcccaattgc atgtttgcta gggaaaatac
      301 ttgtcacttt tccagcgatc ggcgcaacaa cttctcttga tgttggaata acagcaaagc
      361 cgtcacccat cattttttgt gagaataccg gatcttttac ttcagataaa ggaatcactt
      421 ctccagaagc tgcggaaact agttcttgtt ctatacctaa gtattgctta ctacctgctt
      481 gtccatttcc tgtagaaacc atttctggct ctggaatctc tgctcctgat tgcagcagat
      541 cttcgatatc tgatttcaag acatctgctt ttggtccata aactgcttgg actccattgt
      601 ctttgacgat caatcccatc gcccctgctc gtttccaggc ttcttcagtc cctacttttt
      661 ctcgatcatt tacacttacg cgtaaccgag tcatacaagc atcgacatct ttgatatttt
      721 cttttcctcc cagcaaatga atgatctgga ccacttgctg atctgctgtt cctgttccgc
      781 tgttagatac aacggtttct tcactgtcat tgtcgtaatt tccgttacgt cccggtgtag
      841 caaagttgaa ttttttgatc aagaaatttg ctaagaaata cgtaacaaca ccaaaagcga
      901 tcacacacaa cacgaagttg ataagatctc cacctagacc cgctttgata gctaacggtg
      961 tccgagtcag caattcgata ttcccaaatg aatggacccg caatggcaag atatctgcca
     1021 tcgcaaatgc agccccttgg atcactgaat aaatgacata taatggcaca gcagcaaaca
     1081 taaacatgaa ctccaatggt tctgttacac ctgttagaaa gacagccaac gctgctgaaa
     1141 aatacattga tttatatctt gctttcttat caggatcgac attacgatac atcgcaaaag
     1201 ccattcccat caagatacct gaagaaccaa tcatttgtcc tactttgaaa cgagccggcg
     1261 tccagttatg caacacaaac tcatattgcg acatatcccc tgcaccttta agattcacta
     1321 aatcagtggc ccaagccaac cataacggat cttgcccaaa tacttgtgtc cccgcctgtg
     1381 cgcctgataa aatctcatac gatccgccaa gctgtgtata gttgatagga atcgtcagca
     1441 tatgatgcaa tccaaatggc aatagtaaac gttctaatgt tccaaataaa aacggtgcta
     1501 gaatcggtgc gctttcttgt gattctgcaa tccataaacc aaaattattg attccagcct
     1561 gtacgtttgg ccacaccaca gcaagaatca atgccacgat tgttgaccat aagataacaa
     1621 caaacggaac aaagcgcttt ccattgaaaa atgacaaagc atccggcaat tttctataat
     1681 tatagtactt attataagcc attgccccga caaatccagc gatgatccct acgaatactc
     1741 ccatattcaa agctggtgct tccaatacac ttgtaaaaaa tcctttaacc atgatcttcg
     1801 taccgaacag agtgtgggta aatgcatctt cattcgcaag catgtcactt gtgacaccaa
     1861 agatagcccc agtgatccga ttgattaaaa taaatgagat cccagctgca aatgctccgc
     1921 ctgctctttc ttttgcccaa cttcctccta ttgctaaagc aaataaaata tgaagattac
     1981 caataattgc ccaaccaata ttttcaatta ctccgcccgt tgtgacaagc ggcgtccaat
     2041 caggattgat caagggaatt gatttcccta tactgatcat caacccagct gccggcataa
     2101 ctgctaccac aaccattaaa gcctttccaa atttttgcca aaactcaaaa ctaaatatct
     2161 ttttcattcc gtttagctcc tttacgcaat cggtttcgtt aaacgtatta tagcgtgcgt
     2221 tttctttttt gtaaatgctt tattttttat ttttctgtct ataccattct ataaaaccta
     2281 taatacgtgt gttcccttct ataattttct cctaaaaatt tattttttta agcttttttc
     2341 tatgaaatat tttcttttgt ctattgactt gtgcaatcgg ttgcgtttat tatgaacgta
     2401 taaaacagct cattaggagg agaaaaatga aacagatcaa acgactattt gctattgatc
     2461 catggaaaat cagcacgaca caattagata aaaaaaatct tcgactgcaa gaatctttaa
     2521 caagtattgg aaacggttat atggggatgc gcgggaactt tgaagaaact tactcagggg
     2581 accaccatca aggtacttat ttggcgggtg tctggtatcc cgataagaca cgtgtcggct
     2641 ggtggaaaaa tggttatcca gaatactttg gaaaagtgat caacgccttg aactttatcg
     2701 cgatagatat ttcgatcaac gatcagctga tcgacttggc ttctttggaa ccagaagact
     2761 tttactggga attagatatg aaaaatggtg tattatctcg tacattcaca atcactactt
     2821 ctactaataa agtacgcatt tccttcgaac gttttctgag cattgtcaaa aaagaatcag
     2881 cttatatcta tttaactata gaaatgcttg aaggtagcgg aacagtcaaa ctcgtatcaa
     2941 aattggatgg aaatgttcaa aacgaagaca gcaactatga tgaacatttt tggaatgaga
     3001 ttgatcgtgg aactatagaa gaaatcggct atttgacgac taaaactatt ccaaacaact
     3061 ttgggattga acaatttact gttactgcag caatgcgcca tttcgttaat ggaaaatcag
     3121 tcatccctga ctatcacgaa gatactttag cattacatgg ttgcttgtct gcagaattat
     3181 ctcaaggtga gacatggtca gtggaaaaag aaattatcgt tcttactagt agagatattc
     3241 cagaagcgca gcaagcagaa aaagctgtct cagaattaac gaaccagaca aacaattata
     3301 tccaagcaaa agaagagcaa acagcggctt gggcagaacg atggaagctt gccgatgtgg
     3361 tcatcgaagg agacgaagaa gcacaacaag gtattcgatt caatttgttc caactattct
     3421 ctacttacta cggcgaagat gaacgattga atatcggccc caaaggattc acaggagaaa
     3481 aatacggcgg agccacttac tgggatacag aagcgtatgc tgtccctctt tatcttgctt
     3541 tagccaaacc tgaagtaact aagaatctgc tgaaatatcg gcataatcaa ctgccgcaag
     3601 caatccacaa cgctcagcaa caaggattaa aaggcgcgct ttatcctatg gtcaccttca
     3661 ctggtgtcga atgccataac gagtgggaaa tcacattcga ggagattcac cgaaatggcg
     3721 cgattgccta tgcaatctac aattatacga attacaccgg cgatacggaa tatttgaaaa
     3781 atgaaggact ggaagtttta gcagaaatcg cacgcttctg ggcagaccgt gtccattttt
     3841 caaaacgagc aaataaatac atgatccacg gtgtgaccgg cccaaacgaa tatgagaaca
     3901 atatcaataa caattggtat acaaatacga tcgcagcttg ggtcttgcgt tacacgcgag
     3961 aaaattattt aacttatcaa aaagaagctg ctatcactat cagcgaagaa gaactagcca
     4021 aatggaaaga tatcgcagag aacatgtact tcccagttga cgaagaatta ggaatctttg
     4081 tccagcatga cacattctta gataaagatc tcatgcctgt atccgatatt cccacaagcg
     4141 atcttcctct gaatcagcat tggtcttggg acaaaatcct tagatcttgc ttcatcaagc
     4201 aggcagatgt actacagggg atttatttct tcaatgatac ttttacaaaa gaagaaaagc
     4261 aaaaaaactt tgatttctat gaaccaatga ctgtccatga gtcttccctt tcgccaagta
     4321 tccattcgat tttagcagca gaattaggaa tggaaagcaa agctgtagaa atgtaccagc
     4381 gaacagctcg cttggatttg gataattaca ataacgacac agaagacggc ttgcacatca
     4441 cttccatgac tggtagctgg ttgtcgattg tccaaggatt tgctcaaatg aaaactgccc
     4501 atgatcagtt gagattcgca ccatttctgc caaaaacttg gaaagcttat tctttccata
     4561 tcaattatcg cggacgttta ttgttcatct cagtcacaga tgaacaagtt actttcgagt
     4621 tattagaagg ttctcctcta gaaatttacg tctatgaaca gtcttattct ttagcggatc
     4681 gtctagaaat aacgctaaga aaggaggaag caaatgttta aaggggtatt gtttgattta
     4741 gacggtgtca ttaccgatac tgctgaattc cattatcgtg cttggcgaaa gcttggtcag
     4801 gaaatcggca ttacgatcga ccacacattc aacgaacagc taaaaggcgt cagccgagaa
     4861 gactcactct ctttgctttt agcttatggc ggaaaagaac actctttttc aaaggaagaa
     4921 tttgcagaat tagcgaaaag aaaaaacgat tattatttag agatgatcca gacgatcgaa
     4981 ccaaaagatg tcttccccgg ggtcgtgcct ttactggatt ctttaaaaga agaaaaaatc
     5041 aagattgctc ttgcttctgc aagtaaaaac gggccattcc tacttgaaaa aatgggtctt
     5101 acaccttact ttgatgctat tgccgacccg gcagaagcaa caaacggaaa acctgcgcca
     5161 gatatcttcc tccttgcagc aaaagctgtc ggactaaaag cagaagaatg tatcgggatc
     5221 gaagacgcgc aagccggtat ccaagccatc ctctcaagtg gcgctcaacc aattggcgtg
     5281 ggacgtgctg aagaccttgg agaggagatc ccgcttgtcc ctgatacgac ttttcttacg
     5341 atcgattact tgaaagaaaa gtggcatgac catggataat ccagagatct ccgtcgtacc
     5401 agttccagag actgaaataa agaaaatcac attgaagaac gactacttag aagtcgttct
     5461 tctcaattac ggtgcccgcc tgcaccaaat cttcgcacca gacaaagaag gaaaatcaga
     5521 aaatgtttta ttgtcttacg atcgtttcga agatgttcta acagacaaaa gcttttttgg
     5581 agcaactgtc ggacctgtag ccggaaggat ccgggatgct tcttggggtg agcatagatt
     5641 ggagaaaaac tgcggatcac accatatcca cggtggcacg aaaggctggt ctttccaatt
     5701 ttgggaagta gaagttttca aaactccatc atcaataggt gtggtctttt acttaaaaga
     5761 tgaattttct acctatccag gaccaatcac tgctacgatc acttaccgtt taacaaaaaa
     5821 cgaattagag atgacaacct ctgctagtag ccttgttgaa accatctgca atccgaccaa
     5881 ccatgcctac ttcaaccttt caggaaatgg aacaagagac atctatgaac atcagttaac
     5941 ggtcttttta gatggtgtgc ttgaattaga tcaagaaaaa ctgcctactg gaaattggac
     6001 aaaaaaagaa aaactgccaa tcgactttag aaaatcccca acactgcaag agatccttgc
     6061 atgttatcca gatggactag atgatgtatt tcttttgcat catcctaggc tttccaaaac
     6121 ttccttacaa ttattcgaga agcattctgg tcgccaaatg actatagcaa cttctaataa
     6181 aagtatggta ttattttcta cgactggatt tgaagctgat ttctctgtca atggaaaaca
     6241 gatgcattca aactacggat tagcaatcga accgcaagaa atccctgata tcgttcattt
     6301 ccccaaattt ggatcgatca acctccatcc tggtcaagaa cggatcagtc agacgattta
     6361 tcggttctct gcacaataac aagagaaagg tgtttttatc atggctatta ccgtaaaaga
     6421 tgtggcgaaa aaagccggtg ttgccacctc gaccgtctct cgtgtcatca acgatcatcc
     6481 tagtatttcc gagacaacaa agaagaaagt ccgaaaaatt atggatgaac taggttatgt
     6541 tcctaacatg actgcccgca atctaggaaa acgcatttcc agtgccatcg gcgtaatctt
     6601 accccctctt gattcaaaag agcggatcgg taacccattt tatcttgaga tcatggaagc
     6661 aatcaacgaa gaagcgcgga attatgatat gactacagca atcgctactg ctaaaaattt
     6721 tgaattgctg ttagagaacg tccagcgtat gcatttacaa aaacaagtag atggttttat
     6781 tctagtgtat tcagatagtc aagatactgt cattgattat ttataccaaa accacatccc
     6841 tttcactttg atcggacagc cttatcatca tgagaacgaa atcgtttatg tagataatga
     6901 taaccagctt ctaggtaaac aagcaacaga atttttgatc cagaatggac ataagaatat
     6961 cctgtttgtg acaaacacga ctcatgaaag tttgtatttc gaacgatatt tcggttatca
     7021 aaaagcaatg atgatggctg gtcttcctgc gttatccagt gtagtactag agcatccgga
     7081 ggattatctg tcctttgaga aaacgttgaa agaaaacgaa gccacagcat tggtggtcat
     7141 tgacgatatc ttcgcccttc gcacgatgca gttggcgcaa atgtatggct atgtcattcc
     7201 agagacactc tccgtcatta gcttcaacaa ctcgatcttc tcaacattaa cacaccctta
     7261 ccttacaagt atcgatatcg atatctccga attaggacgt gtcgctatgc aaaagctgcg
     7321 cgaattgatc catgaagaat tgagcagtgg cgtacgtttg gtcatccctc atcgcttgat
     7381 caaaagagag acagtcgttc ctttagaaaa cccatactaa atagctgact cactaaatac
     7441 gctgaccatt tatcctatgg tcagcttttt atgtaaaaga agatactact tctaacacat
     7501 tgtttatctg t
//
LOCUS       DACEUZ010000113         7450 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.113, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000113 DACEUZ010000000
VERSION     DACEUZ010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7450
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.113"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(468..1814)
                     /gene="gor"
                     /locus_tag="IXG44_002147"
     CDS             complement(468..1814)
                     /gene="gor"
                     /locus_tag="IXG44_002147"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HAQ1658850.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(1948..3189)
                     /locus_tag="IXG44_002148"
     CDS             complement(1948..3189)
                     /locus_tag="IXG44_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAQ1658851.1"
                     /translation="MTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAYGLRS
                     RAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISAVIVS
                     DPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVAEIRK
                     NTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFGQERN
                     SLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNVYKAA
                     VDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVYKFVG
                     EVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRAPNPM
                     MILTMPVAQPVKPGDMIRKRK"
     gene            complement(3207..4130)
                     /locus_tag="IXG44_002149"
     CDS             complement(3207..4130)
                     /locus_tag="IXG44_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAQ1658852.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(4351..6843)
                     /locus_tag="IXG44_002150"
     CDS             complement(4351..6843)
                     /locus_tag="IXG44_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAQ1658853.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(6850..7317)
                     /locus_tag="IXG44_002151"
     CDS             complement(6850..7317)
                     /locus_tag="IXG44_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HAQ1658854.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
BASE COUNT         2047 a         1626 c         1193 g         2584 t
ORIGIN      
        1 ggacagctga ttgaacacta tttgttcgga aaaagtgact aactatgaca caattttttt
       61 cacagtccct tttttcactt gatttgattc gtaccagcga acttgacgag aactttcttg
      121 gtttggtaac tatatggtat tcttttaaca tcctcgtttg cttcttctaa tttatttata
      181 caaagtcaca cttttttaaa catctgttct tcgatacatt tctatttctt ttaaacatcg
      241 ataccaatat tttgaactta agagtgcata ccaaatggat tcatttactc ttcgtcagtt
      301 acgaagataa tcaaaatctc caacttccct acttatttcg ttttgagaat tatcgaagca
      361 atcttacata aataactaaa aaacaaaaaa ccagaaaatc agtatactga ttttctggtt
      421 ttacatggtt tgttcaataa aaagctgttg gaactgttaa tattaaatta tcgcatcgtt
      481 acgaattctt ccgctcctgt tggatggatg gctactgtat tgtcaaagtc ttctttagtt
      541 gcacccatct tgatagccac ggcaaaacct tgcagcatct catcgacacc aacaccaata
      601 gcatgtaagc cgacgattct ttcttctttt ccgacacaga tcaatttcat ttcgcatttt
      661 tggcgataat catttaatgc aaagtacatt ggggtaaagc gtgaacgata aactttcaat
      721 tcattttcgc catatttttc caatgcttct tcttctgtca aaccaattgt tgcaattggt
      781 ggatgcgtaa agacgactgt cggaacaagt ttatagtcaa gataagaatc tttctttcca
      841 ttaaataaac gttctgataa acgtctacca gctgcgattg caacaggagt cagatctaat
      901 ttaccgatca catcaccaac agcaaaaata tgttcatccg ttgtattttg gtatttatct
      961 actttgataa accctttttt gtccaattcc acatttgtat tttccagacc taatttttcg
     1021 gtattcggta cacgtccacc ggcaaaaagt acacaatcac cagttaaggt ttcaccattt
     1081 tcaaatagca ccgtatattc gtcattgttt ttagtaattt ctttagcaat atgatgtgcg
     1141 taagttttta ttccagcttc ttggtacatt tggatcaaat tgtccgaaag cattttatca
     1201 aacgttctaa gtggtcgttc ttttcgatat gcccacatga cttcactacc taaaccacga
     1261 aatacacctg aaagttcagc cgcaatgtat ccagcaccta aaacgattgc tctcttaggt
     1321 aactctgtta attcaaaaaa tccatttgaa tcaattgcgt attctccacc aggaatggtc
     1381 attttacttg cacggcctcc agtagcgatc aagattttag gagcacgata ggtttcaccg
     1441 tttacttcga tcgtttgact atcgataaaa gtagcgtaac cttttatagc ctcgacatga
     1501 ttgctatcta atcctctttg ataagcaccg tgtagaaaat caatatattt ttctcgtctt
     1561 tctactagac cggcgaaatc aacatttttt aaatctgctt ggataccgta acttgaggca
     1621 tctcgttcaa ttgtttccaa aatcgtactt gcttgccaca taactttttt aggtacacaa
     1681 ccgacgttga cacatgttcc gcccaattca ttcgcttcaa tcagcaaaac ttttgcgcca
     1741 tgcatccctg ctctgtttgc tgaagcaata ccgccagagc cgccaccaat tacgatgtaa
     1801 tcatatattt gcattttgtt cctccttatt gaagagatca atcaaaacct ttatttaacg
     1861 atcctttttc tacagaaaac aagtgtaaca cttttcttga attttgtatg cgaagatgct
     1921 taaaacaggg atatatcagc tttttcctta tttacgtttt cggatcatat ctccaggttt
     1981 caccggctgt gcgactggca tagttaaaat catcattgga tttggtgctc ggtcaatgga
     2041 ttcgccttct tcattatgca taacttcaac ggtttgactg aaatgactga atcctggacc
     2101 ataaaattcg atttcatctc ctacagaaaa caagtttctc tgacgtatcg tagcaatctg
     2161 tgttgcctca tcataagata atacttcacc gacaaattta tatactggga ttttacgacg
     2221 ttcaccgaat aattgttcgt tttcagttgg tgtattgtag taaaaacctg tagctaattc
     2281 tcgttgtgct actttccata actcgtcgat ccattcttgc ttgcagacat agttttcagg
     2341 atcttccata taactgtcta cagccgcttt atagacattt gacaccgtag atacataatg
     2401 gatcgatttc attcttcctt cgatcttgaa gctgtctacg ccgttttcaa tcaattctgg
     2461 aatatgttcg atcattgaca tatccactgc actcatcgag aattcttctt ccacttcgcc
     2521 tgtatcagtt aaactgtttc gttcttgacc aaaaggcata tcgaacaatc catacttcca
     2581 gcgacaagat tgagaacatc cgccacggtt tgcatcccgc atcgacatat ggttggacaa
     2641 tgtgcatcga ccagaataag agatacacat ggcaccatgg ataaatgctt cgatttcggt
     2701 atccgtattt ttacgaatct ctgccacttc ctccattgaa acttcacgag ctaaaacgac
     2761 acgttccaac ccttcttctt tccagaattc caatgtctca aaattagttg ctgaagcttg
     2821 agtggataaa tggatcggca gacctggggc ttctgtcgca caaatttcga tcaaagctgg
     2881 atcagaaaca atcactgcag aaataccgac atcacgcaaa ctgcggaaaa attcccctgc
     2941 tccatcttca tttccttcat gggtgaccat attagcagca acgtatacct tcgcatcatg
     3001 ttcttttgca aaagcaactc cttcagccat ttcttcataa gtaaaattac ctgcacggct
     3061 tcttaaacca tatgcattcc cgccaatata tactgcatct gctccataat ggatcgcggt
     3121 ttttaacttt tccagtgttc ctgctggcgc taggatttct ggtcgtttca attttctttt
     3181 tactgtcatt tctttcatca tactccttat ttgatagcag aaggatcaat ataaaagaat
     3241 cctgtatcta attcacggtt ttcaggatgc aatttactga tttcgctagt cattttttcc
     3301 gctttttctt gatcccagct accttctaaa atttgttttt ttgcttcggc gaatagtcga
     3361 gtgatgttta cgaatttttc gcctggagta aataatccgt ccaatttcca tgtacagtaa
     3421 tgatgctgat aaagttcatt caattcattc gagagattca gatcattgct ggcaaaaata
     3481 tgtgtgccat gactatcttc ataaatagaa taatgtgtct cttctttttt aggttccgag
     3541 ataaaaagac ctctttcacg atctttttgt tcgtcttgtt tcgtatagtt atagtaattc
     3601 tgtagcagtg gacgtttgga ttgatggatg catgtagcac catacacaag ggtttctaca
     3661 ggaatatcga gtttttcttc catcgccttc atctcttcaa aaggaacttc acgagcaagt
     3721 actgctccaa ctgcgccttt tttcgcccag aaattgattt gacgtgcgct cgttactaat
     3781 gtttcagcat cataaatata cggtatacgc aattcaggat tttttttcat cgtataaata
     3841 atacctgtat caccaactgt gatcacatct acgtttattt cttttaaaaa agccaaatat
     3901 tcaggtacat ttttcatttt ttcaggatga atgatcccat ttacagcaat attcgctttt
     3961 ttaccatact tgtgtgctaa ttctaccaat tgtcgctgtt cttctcgtgt aaatgaagca
     4021 ggcagacgca atccaaattg ctcttcaccg aaatatagtg tatctgcacc tgcagcaagc
     4081 aactgttctg cttgttcaat agtttctact gtggttatga gttcgatcat aattttctcc
     4141 tcctacaact tcataatagt agcatagctc taaaaacttt ccaatacttc ttctatttaa
     4201 agtcggattt ttgaaaatta tttcacaaaa aatcaaaata aagttcgata tattctgtcc
     4261 aaatataaaa agagattgtg acaaaaatct tgtcacaacc tcttaatcaa gtcttactat
     4321 tataaaaatt cagctacaca ggccaagtaa ctatagggaa cctactgttt cctttttagg
     4381 tccgcgaaca ttcaatgtga tcttaccttt tgaagcacct actgttacgt ggtcacctaa
     4441 acgaatttct ccgccaagca aagcctcgct caatcgatct tcaatttctt tttgaagtgc
     4501 gcgacggatt ggacgtgcac catattcagg atcaaatcca gctttcccga tcacatcgat
     4561 tgcagatggt gtgattttca cttgaatatc ttgttctttc aatcgtttga taatcgcttt
     4621 gctcatgatt tggacgattg tatggatttc atctttactc aatgaatgga agacgacagt
     4681 ctcatcgatt cgattcaaga attcaggtcg gaaagctttc tttaactctt ctaagatacg
     4741 tttttgcatg gctttgtgat ctttagtaac atcttggaca ttgaatccga catttttttc
     4801 ttctcgaatt tgtgtcgctc cgatatttga agtcataatc atgattgtat ttctaaagtc
     4861 cacttttcta cctttagaat ctgttaaatg accatcatcc aatacttgca gaagtaaatt
     4921 gaaaacgtct ggatgtgctt tctccacttc atctaagaga atcactgaat atggtttttg
     4981 acgaattttt tctgtcaact gtccaccttc atcataccct acgtatcctg gaggagaccc
     5041 gattaatcgg cttgtactgt atttttccat aaattctgac atatcaacac ggatcagggc
     5101 atcttcactt ccaaacatga cttctgaaag tgctttggcc aattctgttt ttccgacacc
     5161 agttggtcca aggaacatga atgaaccaat tggacgatca ggatctttca atccgcttcg
     5221 tgcacggcgg atcgcacgtg aaactgcttt gacagcctca tcttgaccaa cgacacgttc
     5281 atgcagaagt ccttccagtt ctaacaagcg ttcgctttct tttttctcca actgctgtag
     5341 agggacacca gtccactccg atacaactgt agcaacatct tcttctgtta cacgatcaga
     5401 ataaccttgc tcctgttttg cttcaattgc agaaactttt tgcagctttt tacgtagtgc
     5461 tttttcttgt ctgcgtaatt gagcagcatt ttcaaagtct tgtttttgga tcgctgcttc
     5521 cttttcttgt actagttcat cgatttctag tttgatcaca gttgattttg ttaagtgatc
     5581 ggtttgatcc aaacggactt tcgctgctga ttcatcgatc aaatcaattg ctttatcggg
     5641 tagttgacga gaagtgatat aacgaacaga aagattgact gctgctcgta cagcttcatc
     5701 tgtgatctcc actccatggt gctcttcata tcttgtacgt aatccttgaa gaatcacttc
     5761 tgcttcttca ggagttggct catctacttg aatgcgagca aaacgtcgct ctaatgcaga
     5821 atctttttcg atatattttt gatattcatc tagggtcgtt gctccgatcg tttgtaattc
     5881 tcctcgagct aacgccggtt tcaagatatt cgaagcatca attgctcctt ctgctccacc
     5941 tgcaccaatt aaggtatgca attcatcaat gaataagatt acttgaccat cattatagat
     6001 ttcgtcgatg acctttttca ttcgatcttc aaattcaccg cgatatttcg taccagcaac
     6061 caaagatccc atatcaagca tcatcaggcg cttttcttgc atgtcttctg gcacttcacc
     6121 taaaatgatt ttttgagcta acccttcagc aatggctgtt ttgcctacac caggttcacc
     6181 caccagtaca gggttattct ttgtccgtcg gctaagaatc tggataagcc gtttcacttc
     6241 tctcgaacga ccaacaactg ggtccagctt gttttctttt gctaatttcg tcaaatcacg
     6301 tgctaatgaa tctagcgtag gtgttccttg cggtgtttgc cgtttatctt gtacatttac
     6361 atttctacga cgattcattc cttttccagc agaatcacca gctagcccca ttttttgttt
     6421 cagaacttga cgggcttttg aaaggctgat acctaagttg atcatgattc gagtagccat
     6481 gatactttca tcttttaata agctcatcaa aatatgttct gttccaactt tttgagaacc
     6541 agttttcttc acttcgctgc tagctaaagc aaaaacttgt ttcattcttg gtgaatatgg
     6601 aagaaagact cctttagggt aagaacggat accaccataa ccaatcagat gttcaatttc
     6661 ttcataaatt tccgtttcat tcaaatccat ttcacgtaat acttttccag caattccatt
     6721 ttgctcgacc actaaggcta gtaaaacatg ttctgaacta actgattgat gtttcaaccg
     6781 ttttgcttct tcttgtgcaa tttctaatac tgcttgggct ctttcagtaa aaagttcttc
     6841 catctaatct cacttcccat cttcataact taaacgttcc agaaatgctc ggagaatccg
     6901 agcacgtgta tgatcttcat attcattgaa attcaatgtg ttttttgcaa ttgcactcaa
     6961 catcaagttt ccttcttttt ttgtaattat ttggtcttca taaagttttt gaataatagc
     7021 aaaagcattt ttctcactga ttgtatcatc aaataactga tttatctgct caagtaattg
     7081 ttttttatct gaaatcctta cttttgcaat tcgtatataa ccaccaccac cacgtttgct
     7141 ttctactaga taaccacgtt ggatggtaaa acgggtatta atcacatagt tgatttgtga
     7201 aggaacacag ttaaagaggt ctgccatttc aatccttcga atctcgatca tttcactatt
     7261 ttccagaatt ttctttatat atgattcaat taaatccgag gtattctgat gtgccatctt
     7321 attcattccc tttgactaat attgacctta attataacga atttgataat agaaagaaag
     7381 aagaacgctt tttgttaaat aaatccttta gaatcaacaa aaaaaaatac ggaatatact
     7441 attccgtatc
//
LOCUS       DACEUZ010000114         7387 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.114, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000114 DACEUZ010000000
VERSION     DACEUZ010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7387
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.114"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            362..805
                     /gene="rplM"
                     /locus_tag="IXG44_002152"
     CDS             362..805
                     /gene="rplM"
                     /locus_tag="IXG44_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HAQ1658855.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            821..1213
                     /gene="rpsI"
                     /locus_tag="IXG44_002153"
     CDS             821..1213
                     /gene="rpsI"
                     /locus_tag="IXG44_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAQ1658856.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(1302..2468)
                     /locus_tag="IXG44_002154"
     CDS             complement(1302..2468)
                     /locus_tag="IXG44_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1658857.1"
                     /translation="MSIQKYTTKSGARYSVRLYVRKDENGKQEYYVKKGFKTEKEAKL
                     HEARKKVEIEDTGYHKPSNDLFITVYEEWLPSYRNRVQETTYQRTKDLFRLHILPKFG
                     NKSISKITPVHCQNAVNEWALKFKNIKQLKSYSSQIFEYAIFAELLHRNPMANTNLPK
                     REKKENENYFSLEELKAFWDILNKEEPLKHILIFQLLITTGIRKGELAALHWSDIYFE
                     EQLMYVRKSYATIRNEEKNARRKTVRIQKNTKNISSERILPIDAQTIEMLKIWKKEQA
                     RELLQFGINTNTKNQLIFTYVNADGDINQPLHADYSNNILKRLEKKYKFKHVTIHGFR
                     HTHATLLLEGGASIKETQDRLGHKNAETTLNTYSHVTEKAQRNALEKFIAYTGL"
     gene            complement(2523..3173)
                     /locus_tag="IXG44_002155"
     CDS             complement(2523..3173)
                     /locus_tag="IXG44_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1658858.1"
                     /translation="MSIKTLLNLWFLCYNSLEFTIGGVTLNRIKFYIDSSKKTIKQIS
                     TETGINYTTLTNYYRGERNPRNTETWNILADYFHVPVSYLMGLDETNSIEQPNLDRIK
                     TARLEKKDKIVKNIASLDSDYFTELSNLYFSMTSLLEVKLQEDDSDFLFLKSILEDSF
                     EYTILFAKQKKFINKALNLNIDERKELESELFGNLTQVQVNNLKREITARLISKLQ"
     gene            3248..3451
                     /locus_tag="IXG44_002156"
     CDS             3248..3451
                     /locus_tag="IXG44_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1658859.1"
                     /translation="MITLNDQFIRSLRRHRADLILTKNDAAKLIGINRKTYVKIENGS
                     KESIRASTYQKLVNWLLNDLKSK"
     gene            3459..3785
                     /locus_tag="IXG44_002157"
     CDS             3459..3785
                     /locus_tag="IXG44_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1658860.1"
                     /translation="MASWLIEEIENERRKIMDAGITVMLDKQQTNQLKNYVFEMTKEA
                     IDQARIDTGLERPFLKGKEMAKYLNVSYTTFLKFKRMGLPVILLEKMELFSKEECKKW
                     ILSHQI"
     gene            3940..4983
                     /locus_tag="IXG44_002158"
     CDS             3940..4983
                     /locus_tag="IXG44_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HAQ1658861.1"
                     /translation="MNEKEVESLVEKQGYSNSDIYAELYYQYPIFLNVNEKYKKMKLS
                     SKLVYMLLKDRNRLSLKNNLYDENQKIFLIFEIKNLVEISGMSKNTILSALDELQKVK
                     LLVKRKNGFNKNKMQMFPNFYYLLKPVLCKDDVYKINKITHQENSQKNLIVQNLNYGT
                     NQYSVDSSKFELCDTSMKTRDKNNFADSSKNELELYKSYKDSKDIKESHNDDLFESLS
                     TKNNKLDEELIETFIEENLLKQMWGESIVNCLKLASNSDYRQFDNWYKKFNYAIKSAE
                     KEQKVQLKIIYEICNNSYFVDHVREQLAMTLRDLIRRAKTDHRIKQKNNYIFTSLKNK
                     ALELIKLQKKEGI"
     gene            4985..5203
                     /locus_tag="IXG44_002159"
     CDS             4985..5203
                     /locus_tag="IXG44_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1658862.1"
                     /translation="MIENHLVDKIHCLDQNADTLELTESICSNTIVLDLKAVNDDYVT
                     HIIIDRKIAQKLSESLRKWAEGGNNENN"
     gene            5190..>7387
                     /locus_tag="IXG44_002160"
     CDS             5190..>7387
                     /locus_tag="IXG44_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1658863.1"
                     /translation="MKIIKKKIANRKTNWLPVIFLVLIAVILFTSNIAEGSIFNWFDD
                     TESQTEFLTNPLYQNYLKKYNGNFYSQFGMWIGWSVIKSMFAVVDGIQNMIPEVLDLF
                     SFVESTGLNKVYQAVLSTIVVGLMILSLVIIGYKMITGKGTVDLKSVGMNVVMSISLI
                     LLMPTLIHSGIEFSKTFYNDTTTITNSDEGVAWSLIEQGVTDLAYINKTDQYSMIDKT
                     NDRNNLTKENFWKTDLTQVLTDNVLDKMEKENPIADNLRYELVENSDNEFVATKFSDN
                     FLSTFVEGIKSGYYRYQANLWGISFGLIALAVAYVFSAFIIITAILELAFKRVLGVLV
                     FATDLETGQRSKQVLSDILQCYLTVGFQGFGLSMFAMFVNYLGTGSGSSVNPFIKAVA
                     YVCAVIVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGTGGMNRSKGGKN
                     SGGNDQAEGEERKPERNFGQSLSQKANKTGKTLGYARERGLSGLASDGATMASERATK
                     PFKSMRDMANDTKNKFKEGLDDGTVYAINKNSKPIVAKNKEDETGKYADSMPTRLSDR
                     KDGAKVENADRIMSSSERMREAMKNNAESNINPASAIQQKVKQDIEERKNAMHGQAKS
                     AEVLINQKRQEAKYTPEAMNREEMLRKRVEGRTGANMDEKEALTKERIQEAKNSNTGL
                     EKSVKENLQNSTSSQDRRSVDVRENLQNTTSSQNGNRTVYVRENVQSSGT"
BASE COUNT         2607 a         1102 c         1380 g         2298 t
ORIGIN      
        1 agcctgccgt tcagctccga gtcacaagaa acaattttaa aaaacagctt cttatgtttt
       61 atggatattg tgacttgtga ccgaggagtt ggcaggagaa caccgtttat tcaaaataag
      121 gggttgtgac aagacttttg tcacagcctc tttttgtttt tcattttgac aggaaacgac
      181 ccttttatat aaaagaaaga aaagaagtaa agtttcatat aaatttttca atcaagcaga
      241 aaaacgttga cattgactag attagcgggt aatatgttaa atggtattgt ttgccccaca
      301 aagagccccg gaaactcgaa gtgtatgtca aacatcagaa cttaaattgg aggaaaataa
      361 cgtgcgtaca acatatatgg ccaaaccagg cgaagtagaa cgtaaatggt atgtagtaga
      421 cgcaactgat gttccattgg gacgtctttc aacagttgtt gcatctgtac tacgtggtaa
      481 aaataaacca actttcacac ctcatttgga tactggcgat ttcgtcatcg tcatcaatgc
      541 agacaaagtg aaattaacag gtaaaaaagc gactgacaaa atttattacc gtcacagcaa
      601 ctacccagga ggattgaaat caatcactgc tggcgaattg cgtgctaaaa attctcgtcg
      661 tttgatcgaa acttctgtaa aaggtatgct tcctaaaaac actttaggcc gcaaacaatt
      721 tacaaaattg aacgtatacg gtggagcaga acatccacat gcagcacaac aaccagaagt
      781 tttagacatc acaaacttaa tctaatagga gggaatttca ttggcacaag ttcaatatat
      841 cggcacaggc cgtcgtaaaa acgcagttgc tcgcgtacgt ctagtaccag gaactggcaa
      901 aattactgta aataaaaaag atgttgaaga atacattcca catgctgact tgcgtgaagt
      961 aatcaatcaa cctttcgctg ttactgaaac aaaaggcgca tatgacgtat ttgttaacgt
     1021 aaacggcgga ggatacgctg gacaatcagg agctatccgt catggtatcg ctcgtgcatt
     1081 gctagaagtt gatcctgact tccgtttagc tcttaaacgt gctggcttac ttactcgtga
     1141 cgcacgtatg gtcgaacgta aaaaaccagg tcttaagaaa gcacgtaaag cttcacagtt
     1201 ttcaaaacgt taatcgttgt tataccaacg atttcagaca ctttctcatt gcgagaaggt
     1261 gtcttttttt cgttctggta atgagaaagg taatgacaaa attataaacc agtgtaagca
     1321 atgaattttt ctaacgcatt tcgttgagct ttttcagtaa catgagaata agtgttaagg
     1381 gtagtttcag catttttatg acccaatcga tcttgtgttt ctttgatact tgctccacct
     1441 tctagtagaa gagtagcgtg agtgtgccgg aatccatgaa tagtaacgtg cttaaattta
     1501 tatttcttct ctaaacgttt caatatatta tttgaatagt ccgcatgcaa aggttgatta
     1561 atgtcaccgt ctgcatttac ataggtaaag ataagttgat tttttgtatt tgtatttatg
     1621 ccaaactgca aaagctctcg agcttgttct tttttccaga tttttaacat ttcaatagtt
     1681 tgtgcatcaa ttggtaatat tcgttcggaa gatatatttt ttgtattttt ttgaatcctg
     1741 acagtttttc ttcttgcatt cttctcttca tttcgaatag ttgcatagct ctttctaaca
     1801 tacattagct gttcttcaaa ataaatgtca gaccaatgaa gagcagctag ctctccttta
     1861 cgtataccag tagtaatcaa aagctggaag attaaaatat gttttaaagg ctcttcttta
     1921 ttgagaatat cccaaaaagc ttttagttct tctaatgaaa agtaattttc attttctttc
     1981 ttttcccgct ttggtagatt tgtatttgcc ataggatttc ggtgtaatag ctctgcaaaa
     2041 atggcatact caaatatttg agagctgtaa gacttaagct gtttgatatt cttgaatttt
     2101 aaagcccatt cattgactgc attctgacag tgaacaggag taatcttaga aatggactta
     2161 ttcccgaatt ttggtagtat atgcaggcgg aaaaggtctt ttgtacgttg atatgtcgtt
     2221 tcttgtacac gattcctata agaaggcaac cattcttcat acactgttat aaacaagtca
     2281 ttagaaggct tatgatagcc ggtatcttca atctcgacct tttttcttgc ttcgtgtaac
     2341 ttggcttctt tttcagtttt aaaccctttt tttacatagt attcttgctt tccattttca
     2401 tctttacgaa catacaagcg gacactatat cgtgcaccgc tttttgttgt gtacttttgt
     2461 attgacataa ctaatctctc caatctgata acttggcgag ttggctgatt gaataattag
     2521 ttttattgaa gttttgatat taatcgagct gttatctctc tttttagatt gttaacctgt
     2581 acttgggtta agttgccaaa taactcagat tctaactctt ttctttcatc gatatttaag
     2641 tttaaagctt tattaataaa cttcttttgt ttggcaaata gaatcgtgta ttcaaaggag
     2701 tcctctagaa tactttttaa aaaaagaaag tcggagtcat cttcttgaag tttgacttca
     2761 agtaaagaag tcatggaaaa gtatagatta gataattcag taaaataatc tgagtcaaga
     2821 ctggcaatat tcttgactat tttgtccttt ttttctaaac gagcagtttt tattctatca
     2881 agattaggtt gttctatgga atttgtttcg tctaatccca tcaaataact tacaggaaca
     2941 tggaaataat cagcaaggat attccatgtt tcagtatttc taggatttcg ctcaccacgg
     3001 taatagttcg taagagttgt atagttaata ccagtttctg tactaatttg tttaatggtc
     3061 tttttactgg aatcaatata aaattttatt ctattcaaag taacaccccc tatagtaaat
     3121 tccaaactat tatagcataa aaaccacaaa ttgagtaatg ttttaattga cattactcgt
     3181 ttcgtggttt aaaatgtgct tattaagata aaaaccacaa agtgaataat ttaaaaggag
     3241 gcgctgaatg ataactctta atgatcaatt cattagaagc ttaagaagac atagagccga
     3301 tcttatactt actaaaaacg atgcagcaaa attgataggt ataaatcgga aaacatatgt
     3361 caaaatcgaa aatgggagca aagaaagtat tagagcatct acttatcaaa agttggtaaa
     3421 ttggttactt aatgatttga aatctaaata aatacaactt ggcgagttgg ctgattgaag
     3481 aaatagaaaa tgagaggaga aaaataatgg atgcaggaat tacagtaatg ttagataaac
     3541 aacaaacgaa tcaattgaaa aattatgttt ttgaaatgac aaaagaggct attgatcaag
     3601 caagaattga taccgggtta gaaagaccat ttctaaaagg aaaagagatg gctaaatatt
     3661 tgaatgtatc atatacaact tttttgaaat ttaaaagaat gggattgcca gttattttat
     3721 tagaaaaaat ggagttgttt tcaaaagaag aatgcaaaaa atggatattg agtcatcaaa
     3781 tttaaaaaac gaggtgtttt tttgaataat gatgaagtag aaaaaattaa aaagagtgtt
     3841 cccgtcagtt tgcagatcgg gggaacactc aataataaag ttctgagaaa actttatttg
     3901 aatctattat agcaaataaa atcagaagca ggtgtgacaa tgaatgaaaa agaagttgaa
     3961 agcttagttg aaaagcaagg ctattctaat tctgatatat atgcagaatt atactatcaa
     4021 tatcctattt ttttaaacgt aaatgaaaaa tataagaaaa tgaaactaag ttctaagtta
     4081 gtttatatgt tactaaaaga tagaaataga ctttcattga aaaataactt gtatgatgag
     4141 aatcaaaaaa tttttttgat atttgaaatc aaaaatctcg tagaaatttc gggaatgtca
     4201 aaaaatacaa tcttatcagc actagatgaa cttcaaaaag ttaaacttct tgtgaaaaga
     4261 aaaaatggct tcaataaaaa taaaatgcaa atgttcccaa atttttatta tttgttaaag
     4321 cctgtactgt gcaaagatga tgtttataaa attaataaga taacccatca agaaaatagt
     4381 caaaaaaact tgatagttca aaatttgaac tatggaacaa atcaatattc tgttgatagt
     4441 tcaaaatttg aactatgcga cacatcaatg aaaacaagag acaaaaacaa ttttgctgat
     4501 agttcaaaaa atgaactaga attatataag tcttataaag atagtaaaga tattaaagaa
     4561 tcacataatg atgatctttt tgaatcactt tcaactaaaa ataataaatt agatgaagaa
     4621 ttgatcgaaa catttattga agaaaatctt cttaaacaaa tgtggggaga aagtattgtg
     4681 aattgcttaa aactggctag caatagtgat tatcgtcaat tcgacaactg gtacaaaaaa
     4741 ttcaattatg caattaaatc tgctgaaaaa gaacaaaaag ttcaactgaa aattatctat
     4801 gaaatttgta ataatagtta ttttgtagat catgttagag aacagctagc aatgacttta
     4861 cgtgacttga tacgaagagc aaaaacagat catcgtatca aacaaaaaaa taattatatc
     4921 tttacttctc taaaaaacaa agcgttagaa ctaatcaagc tacaaaaaaa agagggaatt
     4981 taaaatgatt gaaaatcatc tagtagataa gattcactgt cttgatcaaa acgcagatac
     5041 tcttgagtta accgagagta tctgctcaaa tactattgtt ctcgatttaa aagctgttaa
     5101 tgatgattac gtaactcata tcataattga cagaaaaata gctcagaaac tctctgagag
     5161 cctaagaaaa tgggctgagg gaggtaacaa tgaaaataat taaaaaaaag attgctaata
     5221 gaaaaacaaa ttggttaccg gttatttttc ttgttctaat agctgtgatt ctttttacga
     5281 gtaacattgc agaaggcagt attttcaatt ggtttgatga tactgagagt caaactgagt
     5341 ttttaactaa tccgctttac cagaattatt tgaaaaaata caacggaaat ttttacagcc
     5401 agttcggtat gtggataggc tggtctgtta taaaatcaat gttcgctgtt gtcgatggca
     5461 ttcaaaatat gattcccgaa gttttagatt tgtttagctt tgtagaaagt accggattga
     5521 acaaagtcta tcaagctgtt ttaagtacca ttgttgttgg cttaatgata ctttcactag
     5581 tgattattgg ttataaaatg attacaggta agggaacggt tgatctaaaa tctgttggaa
     5641 tgaatgttgt aatgtcaatt tcattgattc ttttaatgcc aacgttgatt cacagcggaa
     5701 tagagttttc taaaacattt tataatgata cgactacgat tacaaattcg gacgaaggcg
     5761 tggcgtggtc gctgattgaa caaggggtta ctgatttagc ttatatcaat aaaacagatc
     5821 aatattccat gatcgataaa acgaatgacc ggaacaatct aacaaaagaa aatttttgga
     5881 aaacggatct tacgcaagta ttgacagata atgtactcga caaaatggaa aaagagaacc
     5941 caatagctga taatctgcgt tatgaattag ttgaaaattc tgataatgaa tttgttgcta
     6001 ccaaattttc ggataatttt ctgtcgacct ttgttgaggg cattaaatca ggatattatc
     6061 gctatcaagc aaatctatgg gggatttctt ttggattgat tgctttagcg gtagcgtatg
     6121 tttttagtgc tttcattata attacagcaa tactcgagct agcgtttaaa cgcgtacttg
     6181 gagttttagt atttgctact gacttggaaa caggccaacg atcaaagcaa gttttgtcag
     6241 atattttaca gtgttatttg acagtcggat ttcaagggtt cggattgtct atgtttgcaa
     6301 tgtttgttaa ttatctcgga acaggtagcg gttcttctgt aaatcctttt ataaaagcag
     6361 tagcgtacgt ctgcgctgtt attgtgctga taaagggtag tggaacagtc atgcgttact
     6421 ttggcgtgga tattggttta aaagaaggct atggccagct tgcttcagca tttggcatgg
     6481 gagctatgct gtttcgtaaa ggaacaggtg gtatgaaccg atctaaaggt gggaaaaata
     6541 gtggtggaaa cgatcaagcg gaaggggaag aacgaaaacc tgaacgcaac tttggtcaat
     6601 cactttctca aaaagctaat aaaacgggga aaacacttgg gtatgctcgt gaacgaggat
     6661 tatcaggctt agcttcggat ggagctacaa tggccagtga aagagcaaca aaaccattta
     6721 agagtatgcg tgatatggct aatgatacca agaataaatt caaagaaggt ttagatgatg
     6781 gtaccgtgta cgctattaat aaaaatagca aacctatagt agctaaaaac aaagaagatg
     6841 aaactggaaa atatgctgat tctatgccaa cacgtctttc ggatagaaaa gatggcgcta
     6901 aagttgaaaa cgctgataga attatgtcta gttctgaacg tatgcgtgaa gcaatgaaaa
     6961 ataatgctga atcaaatatt aatcctgcaa gtgcaattca gcaaaaagtt aaacaagata
     7021 ttgaagaacg taaaaatgct atgcacggtc aagctaagtc tgctgaagta ttaattaatc
     7081 agaagcgaca ggaagcaaaa tacacgcctg aagcaatgaa tcgtgaggaa atgttaagga
     7141 aacgtgtaga agggcgtaca ggcgctaata tggacgaaaa agaagcgttg actaaagaaa
     7201 gaatccaaga agctaaaaat tcaaataccg gattagaaaa atcagtcaaa gaaaatctac
     7261 aaaattctac aagtagtcaa gataggcgct cagtagatgt tcgagaaaac ttacaaaata
     7321 ctacaagtag tcaaaatgga aatcgaacag tatatgtacg agaaaatgta caaagtagtg
     7381 ggacaag
//
LOCUS       DACEUZ010000115         7373 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.115, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000115 DACEUZ010000000
VERSION     DACEUZ010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7373
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.115"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(1..1155)
                     /locus_tag="IXG44_002161"
     CDS             complement(1..1155)
                     /locus_tag="IXG44_002161"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HAQ1658864.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            1340..3781
                     /locus_tag="IXG44_002162"
     CDS             1340..3781
                     /locus_tag="IXG44_002162"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HAQ1658865.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(4111..4815)
                     /locus_tag="IXG44_002163"
     CDS             complement(4111..4815)
                     /locus_tag="IXG44_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1658866.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(5002..5247)
                     /locus_tag="IXG44_002164"
     CDS             complement(5002..5247)
                     /locus_tag="IXG44_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1658867.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(5264..6115)
                     /locus_tag="IXG44_002165"
     CDS             complement(5264..6115)
                     /locus_tag="IXG44_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAQ1658868.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            6281..7285
                     /locus_tag="IXG44_002166"
     CDS             6281..7285
                     /locus_tag="IXG44_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HAQ1658869.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
BASE COUNT         2323 a         1324 c         1523 g         2203 t
ORIGIN      
        1 ttatattttg tagtaacgaa tatcattttc gatctttgta atcgaaaaag gtacatctga
       61 agtatattct gcacattctc cgtgttcagg aagcgtctca ctgaaaatcg tttcatattc
      121 gtcaacagtg atccgtgttc gagaatctag catctcttga tgagattgtg cgaagagata
      181 ttcttgatag ttttcttcca gataacctgt aaaaaattca cttactgcac cagaaccgta
      241 gctgaataga ccgatgcgat ctcctggctg tagtgatttc gaattttcca gtagagaagt
      301 tagccccagg tataatgaac cagtgtaaag attaccgatt cgtcggctgt aacggatgct
      361 ttcttcatag cgagccataa gacgttcctg attttcttcg tctgtttggt ctaatacgct
      421 ttgcaatgcc ttttttccca tcttagtata cggaatatgg aaagcaatcg cctgataatc
      481 ttcgagtcct cgacccgaca attctttatg tcgattccaa actttttgga atgattcgat
      541 atacgtagaa ttagataaag gaccatcaac aacaggaaat tcgctataat ctggacgcca
      601 gaaatcatag atatcttctg tcagaaatac gctgtcgtct tcaatcgata aaatacgcgg
      661 gttttgagtg atcatcatcg caacagcacc gacaccttgc gtcacttcac caccgcttgc
      721 caagccgtaa cgagcaatat cacttgctat gactagtact tttcgttctg gatgattttt
      781 gacatattct ttcgccatgt gcaaagctgc ggtgccaccg tagcaagcct cttttacttc
      841 aaaagaacga gcaaaagatt ggattttcag taaatggtgg ataattaccg cactcgcttt
      901 tgaatgatca atcccggatt ctgttccgac gatgaccatg tcgattagct cacggtcttt
      961 ttctgttacg attttgctgg cagcatttgc tcctagtgta atgatgtctt catttgcacg
     1021 attgactgcc atttggtctt gaccaatccc tatatggtat tttgcaggat catccccacg
     1081 gctttctgcc aactctgtca tatctaaata taaattagga ataaaaaagg aaagacgatc
     1141 aatccctatt ttcatttcaa cacctcttta cttatgataa aaaacttact ttactaaggt
     1201 atcataaaaa gattaaaaga aatataaaaa agagaatatt taaggattgt taaaatttaa
     1261 tcttagaaaa aaataactgg aacaagagct atttgttttt acagcaaaga atggtatatt
     1321 tttgtaggag gtggcttact tgaaagaagt cgttatgata gatgctgcaa gaacgccgat
     1381 aggaaagtat cgcggaagtt taagtccatt tactgcagtt gaattaggca cacttgtgac
     1441 taaaggatta ttagataaaa caaaactgaa aaaagataaa atcgatcaag taatatttgg
     1501 aaatgtcctg caagctggta atggtcaaaa tgttgccaga cagattgctt tgaacagcgg
     1561 tctccctgta gatgtcccag caatgacgat caatgaagtt tgcggatctg gtatgaaagc
     1621 agtgatctta gcacgccagc tgatccaatt aggtgaagca gaacttgtga tagccggcgg
     1681 aacagagagt atgtctcaag cacctatgct gaaacagtat cagtcagaaa caaatgaata
     1741 tggtgaacca atttccagta tggtcaacga cggattgact gacgcatttt caaatgcaca
     1801 tatgggatta accgcagaga atgttgcaac acaattttct gtgagcagag aagagcagga
     1861 tcgctatgcc ttgtcgtccc agttgaaagc agcacatgct gtcgaagccg gtgtattttc
     1921 tgaggagatc atcccagtca agatttctga tgaagacgtg ttatctgagg atgaagcagt
     1981 tcgtggaaat agtacattgg aaaaactggg cacgttacgt acagtattct cagaagaagg
     2041 aactgtaaca gcaggaaatg cttccccgtt gaatgacggt gcctctgtgg tgatccttgc
     2101 atccaaagaa tacgcagaaa ataataatct gccttattta gcaaccatca aagaagtagc
     2161 ggaggtcggt attgacccaa gtatcatggg aatcgctcca atcaaagcca ttcaaaaatt
     2221 gacagataga tcaggaatga atttatcaac aatcgatctt tttgagatca atgaagcatt
     2281 tgcagcttct tctatcgtag ttagccaaga actgcagtta gatgaagaaa aggtcaatat
     2341 atatggagga gcaatcgcat taggccatcc aattggtgca agcggtgcac gtatcttgac
     2401 tacattagct tacggattgc ttcgtgaaca gaagagatac ggtattgctt cattatgtat
     2461 tggtggaggt ttaggcttgg cagtcttgtt agaagcgaat atggagcaaa cacataagga
     2521 tgtgcaaaaa aaaaagtttt atcagcttac gccatctgaa cggcgcagtc aattaataga
     2581 aaaaaatgta ttgacacaag aaactgcgtt gatttttcag gaacaaacac tttctgaaga
     2641 gctctccgat cacatgatcg aaaaccaagt gagtgaagtg gaaatcccaa tggggatcgc
     2701 gcagaatttt caaatcaatg gcaagaaaaa atggattccg atggcaacag aggaaccatc
     2761 tgtcattgca gctgcaagta atggtgcaaa aatctgtgga aatatctgcg cagaaacacc
     2821 tcagcggctg atgcgagggc aaatcgttct atcaggaaaa tcggagtatc aagcagtgat
     2881 caatgcagtt aatcatcgaa aagaagagct gattctttgt gcaaatgaaa gttatccttc
     2941 aatcgtgaaa cgcgggggcg gtgttcaaga tatctctact cgggaattca tggggagttt
     3001 ccatgcatac ttatctattg attttttagt ggatgtaaaa gatgcaatgg gcgctaacat
     3061 gatcaactcg attcttgaga gtgtggcaaa caagttacgg gagtggttcc ctgaagaaga
     3121 gattctattc agtattttaa gtaattttgc tacagaatca ttggcttctg cctgttgtga
     3181 aatcccattc gaacgcttag ggagaaacaa agaaatcggt gaacaaattg ccaaaaaaat
     3241 ccaacaggca ggggaatatg cgaagcttga cccttaccgt gcagcaacgc acaacaaagg
     3301 gatcatgaat ggtatagaag ctgtagtggc agctacagga aatgatacac gtgcggttag
     3361 cgcttcgatc cacgcctacg cggcaagaaa tggattatac caaggattaa cagactggca
     3421 gatcaaaggg gataaactag ttggaaaact aacggtgcct ttggctgttg ccacagtagg
     3481 cggagcttcc aatatcctgc caaaagcaaa agcatcttta gctatgttag atattgattc
     3541 agccaaggaa ctagcacaag taattgctgc agtagggtta gctcaaaacc tggctgcttt
     3601 gcgtgcatta gttacagaag gaatccaaaa aggccacatg ggacttcaag ctcgttcatt
     3661 agcgatctca attggggcta ttggtgagga aatcgagcaa gttgcgaaaa aattacgtga
     3721 agcagaaaag atgaaccagc agacagctat acaaatattg gaaaaaatcc gggaaaaata
     3781 aaaaagcaat atctttagaa gaataaagca agatttacag ttattttatt tactgcagat
     3841 cttgcttttt tctctatcag aaaatatgtt tattattaga attttttata gttaaggtta
     3901 ggcatcttaa gaaatcaggc aactccaaga tatcttcagg tagaagagaa gtcgcctaac
     3961 attgtaggat cataggtgat ttgttggaca ggtcaagctg atatttgagc ctgatgttag
     4021 aaggaatttc tttttacttg ctgtagaaat tattatatga ctaaaaaggc tgggatttta
     4081 aatcccagcc ttttatgaca aatagaattt ttatcgatca gtaatcgctg tcgctgcaaa
     4141 ttgttcgatc aaaggaatca aagtcaattt gactttgtat ttccgttcga gtgctttttc
     4201 gataacgaac tcattttcag tagtcataga aatagttgct aatttacgag gttcatcagc
     4261 atttttttca ttaataacta cttcatgttc attttgttct gtgtaaaatt taggatactc
     4321 agcaacggct tttgaaaaat cagcaaaact taaagcggca ttcgtatcag ttgagcgtgt
     4381 attttcagtt gtgtccgctt ttggtttttc cgttttttct tctggcacac tgactgtttc
     4441 ttctgtaact ggtgtcgcaa cagcttgcgc agttggttca tcgtttttca atcgttgatc
     4501 attccagtaa gacacgattt caataagaag attgtcacgg aatcgttcga aaatcccgtt
     4561 ttctcctgaa cgaattctct ttaatacagt attgaagttg tcatcgattg gcacgttgat
     4621 ccgtgtatct tcatttgttt tcccagctaa tccgatcgtt aagatttgac ggtttttggg
     4681 atcaagcatg acttgtaacg gcaaaccata acgtcgcaag ctctcttctt ctagagaacg
     4741 gaataattgt cctcttacac catccaatga aggacggaat tgatttaata atttttgttt
     4801 ctgttttttc ttcatatgtt ctctccttcg ctcataccct ttcgattata agaaaaaaac
     4861 agccaaatga aaaggagtaa tgcaaaaaaa cttgcgtata cgcaaattac aacagaaaaa
     4921 tttgcgtatt ttcccgtttt ttgaaagaaa aataaaaaat ggaacggatt atgtatatat
     4981 acataatccg ttccattctt tctatttttt cggtatcaag gcaaaaacag gtagttcagg
     5041 tgaataaatg acttcatgtt cgtgccaggg tttgaccatt agcgaatgat cgaaccgttt
     5101 tttcagttct tcgatgattt gatcatgtgt ccagtttctt gcaggaaaat gttggatttt
     5161 ctctgcatcg atcaaaaaga caaaatcgga atgaaaagtc tgggtaactt cttccgagag
     5221 agaattcaag cgttcgattg gatccataaa atcacctttc gacttaaaat tgggtagtat
     5281 ctgggtcttt tgctttgccg actacaccat ctaactgatc gatttgtttc atttcttctt
     5341 ctgttaattc aaaatcaaat aattcagtat tttctttgat tctgcttggt gtcactgact
     5401 taggaagagg aaggaattcg tgttgcagcg accaacgcaa agcaacttgt gcaatcgtct
     5461 tgtcatgtgc ttcagcgatt tgtttcattt caggcacatc gaagatttta ccagtgccta
     5521 atggactata tgcttcgagg atcatatcat gtttttttgc atactcaact acagttgtct
     5581 gtaattcgcc tggtgcaagg aaaacttgat tgaccatcgg cataattttt gctgtttttg
     5641 ctagtgtatc tagatggtgg ggtaagaaat tgctcacacc gattgctttg attttacctg
     5701 cttcatacag ttcttcaaat gctcgccatg tatctgcatt tgcttgttcc caattatcac
     5761 gaaaagcaac agggtttggc cagtggatca aaaacagatc taaataatct gtttggagct
     5821 tcttcaatga ttcttcaaaa gaagacatca caagttcata gctgtggttt gcattccata
     5881 attttgttgt caagaatatc tcttctctag gaattccact ttccttaatc gcttggccga
     5941 cgctttcttc attcttgtat ccttgggcag tatcgatatg gcgataacct gcagccagcg
     6001 cttctttaac agaggaaaca gcgacatccc catcaggtgt ttgccatgtg ccgaatccta
     6061 ctaccgggat ttcgtaacca tttgatagac gataagtgct tgttaatgaa ttcatacttt
     6121 cagctccttt tttattcgtt caactataag tgtagcgcat ttttcgaaaa aaagaatcca
     6181 ttttgtttct actcttgaaa tttttgttta aaagaaaatg attttgattc tggtattttt
     6241 gatttttttt ccgtaaacta agacagaagg gaatgagaaa atggatatct atttaaaaaa
     6301 agcaattctg catattattg acagagaaac tggttctcca gtcttttcac aaaaagagct
     6361 ggatctgaca aaagaatata ttcgtgattt tcttcaaaag aagatccaaa aaatctcttc
     6421 tgctcaaaca aaaactggac aactagcaga agaaagcatg atcgttgaat cacttaaaat
     6481 cgctcaagaa aattttgtgg aagcaagtga agcaatcgtt cagcggtggt tcgatatcta
     6541 ccaagaaagt gaagaagcac ctagtgcaga tgtatttgtt gtcttgtatg aattagatac
     6601 agtgatgtac ttatctttat tgaaagtcaa ttatcgtgag gcttacactc actttgttga
     6661 agcagacgaa gcagggatcg acaaccgctt gattttgaat cgagcaattc taggggcgaa
     6721 aacacaaaaa gcagatgaag cattcgctgt gaatttgact gattggacct acgaattaat
     6781 tgaaaaaaaa tatgagtttt ctggcaaaaa agagctttat ttttctggaa gagtgatcga
     6841 aagtgttcct gcaccttctt tagaagaaaa tgtaaaagtg atccaaaaag tagccaagca
     6901 tcttggaaaa aaatttgaga cagaagaatt tgatatcatg gctgatttga aagaagctgt
     6961 ctatgatacg atagaagaaa aaggtcggtt ggatcatgag atgattgcgg aaaaagtctt
     7021 caaggaaaac atcacggcaa aaatggcttt taaagaagaa gtacaagagc aaggatttgt
     7081 cccagaagca cctcctgtaa aagaagtaca agaaatttct gagaaaaaat ttggaaaaca
     7141 aaaattcaag ctatcaaacg ggatcgaact gatcgtacca gtagatgtat atcgcaaccc
     7201 agatttgatt gaatttgtga ataatccgga tggtacgata tccgtgatga tcaaaaatgt
     7261 ggatgaagta ttgaatcgtt tataaaaatg actagaatat taggtgttca agtaccagtg
     7321 atcagttaaa tgaattgtgt caagagaagg atcggaaaag acgaatataa caa
//
LOCUS       DACEUZ010000116         7349 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.116, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000116 DACEUZ010000000
VERSION     DACEUZ010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7349
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.116"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(<1..2269)
                     /gene="esp"
                     /locus_tag="IXG44_002167"
     CDS             complement(<1..2269)
                     /gene="esp"
                     /locus_tag="IXG44_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HAQ1658870.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKDFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDI"
     gene            complement(2886..3320)
                     /locus_tag="IXG44_002168"
     CDS             complement(2886..3320)
                     /locus_tag="IXG44_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1658871.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFM
                     DYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
     gene            complement(3337..4542)
                     /locus_tag="IXG44_002169"
     CDS             complement(3337..4542)
                     /locus_tag="IXG44_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HAQ1658872.1"
                     /translation="MFSDKEVLLKSLHVCLKLPIVVFNSEFSIIKEYRSDRTTYFFYD
                     YKKLLTDNIKKTKDFHFFTGDFNEMFLLYMFKTRYYLFGPFRANNIDKVFFKLKMNNL
                     NVAMADRERLYNSLQNLTLYSLGDIRDILILVHYFFTGKIEDLFHEPLIEYTENLSKT
                     IEQIKIDNLLSQNYDPEIYLFLYENKILEYVKNGDIRNLENMVFNLSNGIIPSVSGDT
                     IRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIV
                     YYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSVSETKLRESFKNEMH
                     ITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLG
                     LVDNFNNIS"
     gene            5236..5622
                     /locus_tag="IXG44_002170"
     CDS             5236..5622
                     /locus_tag="IXG44_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ1658873.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            5716..5907
                     /locus_tag="IXG44_002171"
     CDS             5716..5907
                     /locus_tag="IXG44_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1658874.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            6421..6753
                     /locus_tag="IXG44_002172"
     CDS             6421..6753
                     /locus_tag="IXG44_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ1658875.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            7049..7204
                     /locus_tag="IXG44_002173"
     CDS             7049..7204
                     /locus_tag="IXG44_002173"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HAQ1658876.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
BASE COUNT         2450 a         1293 c         1056 g         2550 t
ORIGIN      
        1 aaatatcttt acttacagtt actgctaaat cggtcgtgcc tacagaacca tcttggtaaa
       61 caattttaat aggaattgtt ccagtaatcg gctccgattc tcctacacct ttagctggag
      121 tgaatgtaac aataccagtg tttgcatcaa cacttgcatt accgaagtcc ttattataga
      181 catctgaaat tgaaaaggca tctttagcta aaggaacatc tggtttatca aatgtaggtt
      241 ttccactttg aactgtcgtt tctggagttg ttgttatagg tacgtatgtt gcatcatttt
      301 ccatagcttg agaagctttt acagcaaact ctacatccac gtcgaaagtt cgatttccga
      361 tcttaacagt tacaatacct ttcgtactat cagtaacgtt agaagtatca ggctctgttt
      421 tccatgaata ctctgcatct tcaggtaagt ttttattatt ccgaattgat tctttagcat
      481 ctggtttaaa tccaactacc acggtttgtt tatcaaccgc ttttggtgat tccttaataa
      541 cggttgaacc ttcttctggt ttatcaaaac ctggagaaac gatttggatt tttaattcat
      601 ctttcgcgat taatttactt gaatctacac ccgtaaattc aaattctaca gtatctgaaa
      661 ttggagcccc atctttttca tttggagcga tagttttttc tgctaataca gtaccttctt
      721 tatcaacaag ttgggctttg tgacctgttc ccgctaactc gtggatgaat actttcccct
      781 taactgttgt gtcaacatca tatacttctt ctaattcagg tttcccgata tatggattcg
      841 tgtctccgct ctcttcttta tcgtcagggt ttccatcacc atcaatatct tgcaaataat
      901 aataacctgt tccataagtg ttctggatta attttttatt cgtatccgac aaccaagaat
      961 caaagatgaa atcttcacca aatggtttat tttcttcaac ttgtgctgtt gctttcatat
     1021 cttctaagat ttcatctaat ggcttagtca attgtaaaac atatcgaata acgactggac
     1081 gtggacttgc attagcaaaa tcgataagtt tagttaaatc attattagtt attgaaccta
     1141 tgccataatt ttcatgacct tcccgtcgct cgattgctaa atcagctact tttgttccgg
     1201 attcaaacga aacatcatat gttactttgt cagcctgaac catgtgcatc tgtatacccg
     1261 ctacatatgg aactaatcgt tcatcaattt tgtaattagc ataccaaggc gtgccctttg
     1321 cggaagatga atatgagaaa ttataatttt tcgttaactt atgatctaag attatagctc
     1381 cattagcacc tgtggatcct gaaccatctt ttgtttgagt attaggtttc tcatattttg
     1441 cacttactga tccatgttta aactggtcag gattattttc agatacatct aagctattat
     1501 agaaatcatc tgaatctgtt aaaaagtagc ccgtactatc aatacccggt acaattttat
     1561 catcgtcttg atgacttacc aagtagattc gatacattaa acgatcggtc ttgagattgt
     1621 ctatgcctat ctctttaaaa atttcatcta ggctcttttc aaacgtaatt accccattag
     1681 gagcggtttg cgtatcggtt gtttctgccc cagcaaataa tccatcactt gcacgaatat
     1741 aattgacctt ccaaatattt gtaggtctac ccagcgaatc attaattctt acaaaggtcc
     1801 gtctagaaga tccaattcct ttctttggat cgacctgaat tttttctaca tatttagcta
     1861 atcgttcatc taattgaaga cggatttccc agtttttagt tgatgctgca atattatgtt
     1921 ttccgtaaac ttcaaatcca attgtattgc cttttagtgt gtctccagtt agttcttgag
     1981 gactaaattg taaatccgaa aagttataat ttttcgtata accaggattc tcctgaaaag
     2041 tattccccga agctactttg tcacctactt gtacggagct tatcgctgtt tcaagtgggc
     2101 tgcttggaga aatttcatct tgtgctttaa ctagttcagt tgttcccaag acaagaccaa
     2161 caccaatcaa aacagatgca accccaacac ttaatttttt gatactgtac ttcaatactc
     2221 gtttctttgt tttttctaga aatactctct tattattctt gctaaccatt attcaaatca
     2281 actcccttaa taattacttt cctagtaaga ataaaagata aatctgatac gtattattca
     2341 cagaatagga atcttactaa ccttatgtta actaatttac ttagagaatt tcatgaacat
     2401 tacaacaaaa aaagtacatt tcttcatgtt tttttcggat atttgtgctt tttaaccatt
     2461 cagcagctga aaacatataa aaagatatgt atgtattctt tctgaaatta agttcatttc
     2521 tatgcgatga agcatgtttt caaaatatta ccctttctta gattaaactt cagagtattc
     2581 aaataacgga ataaggagcc ttatcccgca ttactgaatg agactgtgac ataagtaaaa
     2641 aggtgacttc aaattccttc ttcggaattt gaaatcacct tctttataat tataaagtat
     2701 cggtttcaca gcatttctga atgattccta gaatcttaaa cgaatgattg gagttatcaa
     2761 cattttttca ctcgttttta gcgaaacaaa aaatgacatt tgtcacagcc tcattaaatt
     2821 attgattctt tttctttata attgaattgt ccatatttaa agtgtctagt actctacatg
     2881 aataattaat tgctttcact caaatactta taatctatta acttgtagtt gtttaacttt
     2941 tctgaaaaag ttagacccac gtaataatct tcccagcttt ctctttcaat taactgatcc
     3001 aatttttgat aatccataaa ttcatcatgt atcaaaaaga ataaataact tgatatttta
     3061 gcatctagct taatcagatt aaataatgtt tgtttaaaat tgctaccatt aatatgactg
     3121 atagattctc tagcttcttc acataaactt tttactttat taattactac tttgtcatat
     3181 tcatttaaca cactattatt accattactt agacgctcag attctacgaa acaactcaaa
     3241 tcagaaattt cccaaaaaaa ttttttgtta aaatccaatt tcctcaactc ctccaaacta
     3301 cgtaaatgat atagtctcat aataaaataa aacctctcag ctaatgttgt tgaaattgtc
     3361 cactaaacct aattgatatt gcttaggaga tacacctgcg tactttttga atactctcga
     3421 aaagtgtgaa gaatctgaaa atcctaaacc taaagaaact tcactgatag tatggtttga
     3481 tttcaacatt attttagccg tagaaatttt tcgcttagag atataattat aaatcgtaat
     3541 atgcatttca ttcttaaagc tttctcttaa tttagtttca cttacagaaa aataattagc
     3601 tatttccttt acagtaattc ttttatagat atttaagccg atgaattgaa cgacagaaga
     3661 aattaaggga gaaagatgct ctatcttagt ttttccgatt tccttagtat aataaacaat
     3721 cccagaatcc ctcactttta agacctcagg taacgataca gctaattcgt tttcttgtat
     3781 caaagcatct ctactttgat acgcttctat tatatccatt cctaatgtta tagacgtttg
     3841 tgctaacttt tcaaaaacaa tgatagaata atttttttca gaccgaatcg tatctccact
     3901 aacacttgga attattccat tactcagatt aaaaaccatg ttttctaaat ttcgaatatc
     3961 tccattttta acatattcca gtattttatt ttcataaaga aacagatata tttctggatc
     4021 gtaattttgt gataacagat tatctatttt gatttgctcg atagttttac ttagattttc
     4081 tgtatattca attaacggtt catggaatag atcttctatt tttccagtaa aaaaataatg
     4141 aactaaaatc agaatatctc gaatgtctcc taaagaatac aaagtcaaat tttgcaatga
     4201 attatagagt cgttctctat cagccattgc aacattcaaa ttgttcattt ttagtttaaa
     4261 aaaaacttta tcaatattat ttgctctaaa tggaccaaat aagtaatatc tggttttaaa
     4321 catgtataga agaaacatct cattaaaatc accagtaaag aaatggaaat ctttagtttt
     4381 tttaatatta tcagttagta actttttata atcataaaaa aaataagtag ttctatctga
     4441 gcgatactct tttataatac tgaattcgga attaaaaacc acgataggta actttaaaca
     4501 aacatgaaga gactttaata agacttcttt atctgaaaac acttttgttt ctctctttcc
     4561 tttctacatc attaaatagt attttatacg ttatctaaaa tactattcct ctgtcaaaat
     4621 aagctaactt acttcattat aaaatatcca cttatcgctt tcatgtatat tccaccgtaa
     4681 aaagtacatt ttatcaccga gaactcatca tttttcatca tgatgaatac tatttacata
     4741 tatctagttc aaagacaata attattatga tccgttatta tctaagcact atacagataa
     4801 taggaatttt tcgtttctaa atattccaat ttgggaatcc aataaaaatt ttcgttcaaa
     4861 tacgtgtcta ttcgaactct tttctagttg ttaattatga agggggaaga aataaaaaat
     4921 acaatctata cacgaataag aatattatcc aaaaggaggt tttagaaaag atttattata
     4981 cgtaaaaagc tcaagacatt ttcttagcca taaggacatg tttgatattt aagaagatat
     5041 atgaattttc ttttctaagt ataaagataa ctatccatta tcaactaaaa aaacattttt
     5101 gaaaataaac atcagcagtc ctatcaatgc atgtgagctt atagaagtta ttcgcagcgc
     5161 agcataattg cattagcttc ttttcgtgtt ttttgcctct tttcccttta aatcttaatg
     5221 gaggaggtgt gcaatatgtc agataacgac gaacgcgtaa atgcccagtt gattcgattc
     5281 tttgaaaagg tcattttgaa caccgctaat acttattata aaaaacaaga agaaattcaa
     5341 aaacatgaac aacttgatca actcccacca tatttgttta ctaaagatac tataaaattt
     5401 aaacaaccga ttaatgtttt aaatgtgact ctccttgttg aagatataaa attagcaaaa
     5461 atccttcctt tgttgaaaga acctgaacaa atttttctta tcgaaaaatt tatctttgat
     5521 aaaactgata aagaaattgg aaaggttctg gggattacaa gacagggtgc gacaaattta
     5581 aaacatcgac tttacaaaaa aattcaaaaa atgctggttt aatatctgca taataactaa
     5641 ttttgtatgc ttctttgctc gttctcctcc ttcccaaaga aatagattat aagtccatgg
     5701 gaagaaggta gatttatgaa ttctttaaaa gaagatttca ttttagcaaa ggctggaaat
     5761 gaagaagctg ttgaggcaat tctcaaaaga tttagctctt tgattcataa gcaatcctgg
     5821 agaactggaa aatatgatca agattgttat caagaatgta tgcttgcaat atatctagct
     5881 atctcgaaat tcgaaattaa agaataactc aactaaggga gtctgtgaca taagtaaaaa
     5941 gatagtttca aatttcgaac aataaatttg aaactatctt ctttataatt ataaagtatc
     6001 gattttacag cgttctataa tgattcatag aatctaaaac gaatgatttt aattatcaac
     6061 gttttttaat tcgtttttag cttaaaaata acttttgtca cagtctcact cctaaaatta
     6121 ctttatttac gtatgaagtg ggcaggaggt cgaatccaga actgggctat ggtacttcaa
     6181 cgaggacttc tcctctagaa ttgaaaagca tgcgatttac cttccgtaac gacggtagat
     6241 ttattcaact atgtgtccaa tcaaaatttg tctgatttca aaaatagtca agagaatttt
     6301 ggttgttaaa aactcttatc tatcaacttc cacagtttag ttgacaaacc ctgatactaa
     6361 gttgggctat gatacacttt tcttgactat cagttacgtt accggaagga atgaactcta
     6421 ttgagaaaaa catatgatcg taaatttaaa ctaaagattt cacaggatat ccttgagaaa
     6481 aaagtaacta ccaagaaaat tgctgaagaa tacaatatct ctcgtccaac aatttcccga
     6541 tgggtttcag aatatcgtcg atacgggaaa aatgcttttg ctggacaagg aaaaagatta
     6601 cctgataaag cagatttcta cattcttgaa caggaaaata aacgactaaa agaggaagac
     6661 gagtgttcta cgaacacaaa gatcgatacg gatctccagg gatcactcaa aaactttacg
     6721 atgaaggaat cgaaatcaat aaacgtgttg tagctgttct tatgcgtgaa atgaatttat
     6781 gcacaagagg atttcatcga cgcaaatctt ctcatggtag agaaaaaaca atcaaagaaa
     6841 tggtaaaaaa atgttgaatc gataatttga acagaaccaa attgatgctg tttgggtgat
     6901 ggatattact tacattccat gtaatgacgg tcgcctctat ttgtccacct atattgactt
     6961 agcaactcgt attctacgtt actatatggt agattcacac atgaaaaaag agattgtcgt
     7021 ccgtccatta caaatttatt aaggagaatt gcctacggta attcattccg atagaggtag
     7081 ccagcatcgg tcttttattt atcaagagct gttgaacgac caccacatca cacattcagt
     7141 gagccaaact gggactcctg ttgacaatgc agtaattgaa gcgtttcatc gatcaatcaa
     7201 atgagaatta atcgactcca acaaatataa aagtaaaata gagatgaagg tgttaatcca
     7261 agattattta gaagactact acccaaacaa aagaatccat acaaaattta tgatgactcc
     7321 ttgacaattt gaagcgaaga aaataaggg
//
LOCUS       DACEUZ010000117         7344 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.117, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000117 DACEUZ010000000
VERSION     DACEUZ010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7344
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.117"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            140..460
                     /locus_tag="IXG44_002174"
     CDS             140..460
                     /locus_tag="IXG44_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1658877.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            912..1112
                     /locus_tag="IXG44_002175"
     CDS             912..1112
                     /locus_tag="IXG44_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ1658878.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            1259..4048
                     /locus_tag="IXG44_002176"
     CDS             1259..4048
                     /locus_tag="IXG44_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAQ1658879.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLIQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            4095..4622
                     /locus_tag="IXG44_002177"
     CDS             4095..4622
                     /locus_tag="IXG44_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ1658880.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            4643..5833
                     /locus_tag="IXG44_002178"
     CDS             4643..5833
                     /locus_tag="IXG44_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ1658881.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYSLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            5873..7171
                     /gene="asnS"
                     /locus_tag="IXG44_002179"
     CDS             5873..7171
                     /gene="asnS"
                     /locus_tag="IXG44_002179"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HAQ1658882.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
BASE COUNT         2439 a         1340 c         1576 g         1989 t
ORIGIN      
        1 aaaaagaggt tgtgacgtgg tcacgacctc tttttgacga ttatgataca aaaaacatca
       61 tttatgaaca aaatgtgaat aacgggcgtt cttgagatat gattttaatt gggaaaaact
      121 tatcgataag gaggtcttta tgaaaaaaag actgttatgg tgtttgctgt ttctatccct
      181 aagtttaaca agtcagccag tttttgctga agaaacacag cggtctccta ttacaggcac
      241 acgtctgtac aaaaatgttt atgtacagac aacagatctt gattctgcga ttcctgaaac
      301 agaagaacgg tctttgcagc caagatttgg ttacttttgg tactgtccga cctgtgatta
      361 tgaaagttgc tggcatttct tacattctac agctgcagct tttgctttta ctcatactca
      421 gaaatatgat ggacatcatc ctgttattta tgaggtatag atatgagcaa aaaagaaaaa
      481 ctaatctcgt tgatcgtatt ggtttgcctt gttcttctgg taactgtcat aggctatcag
      541 aaataccatg agaacatcag acctactaaa gaatcttccc cagcctcgac cgttgaaaca
      601 gaaccgataa gtagtcagaa agaataattt ttttagttta aagaagaaca tcagagcaaa
      661 aaaacaccaa ctgaacaata tgtcgtttat tttaaaaaaa gttcggtttt tcactttatg
      721 taatcgctga gaaaactttt tttaataaaa aaagtcgaaa aactctagct tttttgtaaa
      781 cggtatgata tactatagga gtagtttttg ttcggtaatc tgtggtagct tgtttcaaat
      841 aaacatcttc cagatataca ccattcaagt aattgcaaaa tatgtgttga tacacaagga
      901 ggatttttat tatggaaaca ggtacagtaa aatggtttaa ctcagaaaaa ggatttggat
      961 tcatcactgc agaaaacgga aacgatgtat tcgttcattt ctcagctatc caaggcgacg
     1021 gattcaaaac tttagaagaa ggccaagcag taactttcga tgttgaagaa ggccaacgcg
     1081 gacctcaagc aacaaacgtt actaaagctt aattaaccta tactaaaaaa taaacgccca
     1141 gacatttgcg tgtctgggcg tttttgtcta ttttctttaa aaagcgatga gagtattttt
     1201 ctgcactttg tagtataatt tcaaaagaac attagaggca ggagaagagg aagatatttt
     1261 gaaaccaaga acttatgcag tcgttgatat cgaaacgact gggacgaatc caaaagaaga
     1321 tcggatcatc caatttggct gcgtcatgat tgaatcaggt cggatcactt cacgtttttc
     1381 gatcgatatt catccagggc ggaagatttc aaaacaaatc cagcatttaa cagggatcac
     1441 gaatcaacgc gtccaaaaag cgccttattt cgaagatgtc gcccaaacga tttacaatct
     1501 tttggcagac accatatttg tcgcgcataa tatttatttt gattataatt ttttgaacca
     1561 tgaactcatg cgatgcggac ttcctagttt gaagatacca ggcatcgata cagtagaact
     1621 tgcacaaatt ttcttgccga cggaaccaag ctttcgctta gctgatttgt cagaaagcct
     1681 tggatttatt catgagaatc ctcatcaggc agatagtgat gccgaagtga cgggtcaact
     1741 tcttttgctg atcgaagaac ggatgagaaa attgccaatc atcacgttag agcagattgc
     1801 gcgattaagt agacacacag gtatggatac gagtcgtttc atttatcatg tgattgaaga
     1861 aatgaaagaa aaaccagagc cacttgatcc ctcattagaa atcgtcgatg ggctagcttt
     1921 gcagaaaaaa gaagtggaac tattcacatc cgtccattat ggcgagcgga catatccaag
     1981 aaaaaaacaa gcaaaagaaa aaatgtttgg taaaacactg atgtatcgaa aagaacagaa
     2041 cagactgatg aatgcggtct acgaccattt cacaaaagat gaatcaaaag atttgatgat
     2101 cgaagcagct acaggaatgg gtaaaacaat cggttatctg ctgccattga gttatttggc
     2161 tacacctgaa aaacccgctg tcatcagtac cgtttcgcta gttttgcagc agcagatcat
     2221 agaaaaagac attccattgc tgaatcaatt gctagatcag cccatacaac ctgtaattat
     2281 caaaagctat cgccactata ttgatttaca gcgttttaaa ggcacacttg tggaaccgcc
     2341 agaacaaaaa caatatgctt tgtatcaaat ggcggtctta gtttggctga cgcaaacgaa
     2401 gaccggtgat ttggatgaat tacatttaac aaatttgaac cattcattct ttgcagatat
     2461 cgcgcacaga ggtattggat ttttagcaag aaatcagtca ttttacgaac aagactttgt
     2521 tcgtttcttg caaaagaaaa tcaggcaaag taattttttg attgtcaatc atgcgttttt
     2581 aatacaagaa acacagcgaa aagaaccatt gcttccagct agtccatttt tgttgatcga
     2641 tgaagtacat catttgccgg aaatcgctga aaaagtaaat gacaaacgat taagtattaa
     2701 ctatttccat aaacaaatcc agcaattaga agaaccaaat cagctgtttg accgaataca
     2761 aagctattta ccgaaagaac atgaagcctt acgctggtta tcgatttatc gagaagaact
     2821 ttctgcgcta tgcgaagcac agatttgtat ggcagaagga ttaatgagtt tagtgagcgg
     2881 caagcaacct aacggacagt atccagaaga agtcatgatg acaaaagaga tgttggatca
     2941 gctgccactg gaatctggga cagccattga tcacctgcag cttttttatc aggagatcca
     3001 gcagctacag tcacaaatgc gtctgacttt tgagattatc caagaaagct ggacaaacca
     3061 gatgcggatc gatttagcaa gtttatatag cttatttgat caattagaca agcagtactt
     3121 gctgatggat caatggctgg aagactggcg agatcatttg atccattggt tcgttcctgg
     3181 gaacacagcg cagcagatga tttttcatct ccatgatttt caagcggcta tgttgccgtc
     3241 gactgtttgg tatcctcgat atgatcgtat tctttatatt ggaggaacgc tgaagatcgg
     3301 ccctaatcga cactatctct ctaagcggct tggcattgaa gcagcacctc ttaaagtgat
     3361 tgcctctcct tatgattatg agaaacaggc aaggatctat gtgccaaaag aggcacctgc
     3421 cgtccatcaa gtcagcaatc aagcatatat cgattattta gagacagcac ttacaggtct
     3481 gatcgaacaa gaagatcggc caatacttgt gttattcact tctcatgatg ttttgcagaa
     3541 ggtctatcag aaaatgcatc tgtctatgct agaaaaagga cgagaaatac tagctcaagg
     3601 gtttggcgga agccgggaga agcttttgaa acgcttcatt ttgtcagaaa aaagcgtatt
     3661 gtttggcgct gacagctttt gggaaggtgt agatttgcct ggggatgctt tgcagatcgt
     3721 tgtagtcaca cgtttaccct tcgataatcc gcttcgcccg atggtcaaag ctaggaacgc
     3781 ttatttagaa tcagagggga tcaatccttt ttaccaggaa tccgtaccta aagcagcttt
     3841 gaagttacgc caagcattag gtcgcttgat aaggacggag acagatcgtg gcgtattgct
     3901 tattttggat cgaagactag tcactgcaaa atatggtgca cggatcatcc atgcattacc
     3961 gaaaaaagta ccgatcaaag agctagctat ggaggaaatc tatcaggaca tccatgaatt
     4021 cttaaagaaa aatgaagaag gaagttagag gagctgtctt ttttcataat taaaatctgc
     4081 tataatgaaa aggaatgaag ataaaggagc gacgaccctt gaagaaaaaa cagacaaagg
     4141 aaaaaacgat taatcgcgtg ttacttggct tgatcgtatt tttattagcg gtcatcgtga
     4201 tcagctcgat tttttacttg cgttccagtc gtccggcggt gcaggcgaag aaagaagcag
     4261 tagaaatcgc aaagaaatat gcaaatcttg aggaagccga tcatttttat tggtttactc
     4321 gaaagaagac atacttttca ctgacaggta caaatgataa aggaaaagaa atcgccgtta
     4381 tcattcctaa atctggtgaa aaagtaaaag tattggatca aacaaaggga ctgactgaaa
     4441 acgaagcaaa acagacaatt gctgcggccc atcctgaaat ccaaatcgaa aaagctgctt
     4501 tagggatgta tgatgatcag cctgtgtggg aagtagtagg aaaagaaaca aatgggaaac
     4561 tgaattatta cctgattggc tttgaatctg gagatgaagt caagacaatc aagggaattt
     4621 aaaaggtaaa ggaatgaatg ggatggagtt atcaaaaaga acaaaacgat tagaaccgtc
     4681 tgtcactttg gcagctgcgg caaaggccaa ggcattaaaa gcaaaaggca gagatgtgct
     4741 gagtttgact gtgggagaac cggattttgc gacaccagaa aacatccaag aagcagcaat
     4801 cgaagcaatc cgcaacggga aagctagcta ttatacacca acagcaggaa tcccagagct
     4861 tcgacaggca atcgttgatt atctgaagaa aaactatgga cttgtctatg aaccttcaca
     4921 aacagtcgta acagacggag cgaaatttgc tttgtatacc cttttccaaa cgatccttga
     4981 cccgcaagat gaagtcatca ttcctgtgcc ttactgggtc agttacggtg agcaagtaaa
     5041 actggcagaa ggacttcctg tctttgtgaa aggggaagaa tccaatagct ggaaagttac
     5101 tgttgaacag ctagaacaag cacgttcaga aaaaacaaaa gccgtaatca tcaattctcc
     5161 atcgaatccg actggaatga tttatagcaa gaatgaactg caggcaatcg gtgaatgggc
     5221 agtaaaaaat gatattttga tagttgctga tgatatttat ggtcgtttag tttataatgg
     5281 caatgagttt actccaattg caacgatttc agaagcaatc aaaaatcaga ctatcatcat
     5341 caacggcgtg tctaaaacat atgcgatgac aggctggcgc attggttatg cggtgggaaa
     5401 tcctgtgatc atcaatggaa tgatcgctgt tgcttcccag tcaaccagta atccgacagc
     5461 agtcagtcaa tatgcagctg ttgaagcatt gaaaggcgag caagatacag tggaagaaat
     5521 gcggaacgcc tttgaagaac gcctgaatac gttatattcg cttgtggcag aactgccggg
     5581 tgtttctctt gaaaagccac aaggagcttt ttatcttttt cctaatgtaa aagaaacgct
     5641 acgcatgtgt aaatatgaaa atgtcacaca atgggtagaa gatcttttag aagaaacagg
     5701 ggtagcgctt gtgactggcg aaggctttgg ggcaccagaa aatgtccgca tgagttatgc
     5761 tactgatcgc gctacgttag aagaagcggt acgcagaatc aagcaattta ttgagagtaa
     5821 aagtcaaaat taatcctaaa gaaattcaag gaatataaaa ggagagtcat acgtggaaca
     5881 aattcaaatc atcgattcga aaaaccatgt gggagaaaca gtaacaatcg gcgcttgggt
     5941 cgcaaataag cgatcaagcg gaaaaatcgc atttttacaa ttaagagacg gaacagcctt
     6001 tttccaaggt gtagttgtaa aaagcgaagt gtcggaagaa gttttccaaa cagctaaaaa
     6061 tctacaccaa gaaacatccg tgttagtaac tggagaaatc cgtgaagaca gtcgttcaaa
     6121 atttggttat gaaatcggaa ttacaaatgt ggaagtggta agcgagagcc acgattatcc
     6181 gatcacacca aaagaacacg gaacagactt cttgatggat catcgtcatt tatggctgcg
     6241 ttcttcaaga caacatgcga ttatgcagat cagaaatgaa atcattcgtg caacgtatga
     6301 attctttaat caacatcatt ttgtcaaaat tgatccgccg atcttgactg gttctgcacc
     6361 agaaggcact acggatctat tcgaaacaaa ttactttgat caaaaagctt atttatctca
     6421 aagtggccaa ttatacatgg aagctgctgc tatggctttc ggaaaagtct tttcattcgg
     6481 tccgactttc cgtgctgaaa aatcaaaaac acgacgtcat ttgatcgaat tctggatgat
     6541 cgaaccagaa atggctttca ctcatcaaga agaaagttta gaagtacagg aacaatatgt
     6601 agcattctta gtacaaaatg ttttagatca ttgcgaatat gcattagatg tactaggacg
     6661 agacaaagaa gtcttaaaaa cttatacaaa attgccattc cctcgtatta cttatgatga
     6721 tgcgatcgaa ttactgaaga aaaatggatt tgatgacatc gaatggggcg aagactttgg
     6781 ttcacctcat gaaacgttca ttgcgaattc attcgaacag cctgtcttta tcctaaacta
     6841 tccaaaagcc atcaagccat tctacatgaa accacatcca acaagagaag acgtggtgat
     6901 ctgtgcagat atgatcgcac cagaaggtta cggtgaaatt attggcggaa gcgaacgggc
     6961 aacagactat gatttcttgc tagaacaaat ccgtaatcac ggtttggatg aaaaagaata
     7021 ttcatggtat cttgatctac gtaaatacgg ttctgttccg cactctggtt ttggtctagg
     7081 tcttgaacga acagtcactt ggattgctgg tatcgaccat gtacgtgaag ctattccatt
     7141 cccacgtttg ttgaaccgaa tttatccata agaggtgatg aaaaagctgt tctgcatcaa
     7201 gtaattagaa cagccaagca aaaacagctg ttcatgtttt tgaattggcg ggacttatta
     7261 ccgcagatgt aagcttttga ataccgttta gtggagaggt tgtgaaaaag ctgtttagtt
     7321 tcgagtatta agaaaaattt tgtg
//
LOCUS       DACEUZ010000118         7187 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.118, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000118 DACEUZ010000000
VERSION     DACEUZ010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7187
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.118"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(459..1088)
                     /gene="udk"
                     /locus_tag="IXG44_002180"
     CDS             complement(459..1088)
                     /gene="udk"
                     /locus_tag="IXG44_002180"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HAQ1658883.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1172..1867)
                     /locus_tag="IXG44_002181"
     CDS             complement(1172..1867)
                     /locus_tag="IXG44_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="noncanonical pyrimidine nucleotidase, YjjG
                     family"
                     /protein_id="HAQ1658884.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1880..2596)
                     /locus_tag="IXG44_002182"
     CDS             complement(1880..2596)
                     /locus_tag="IXG44_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HAQ1658885.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSILYK"
     gene            2926..3534
                     /locus_tag="IXG44_002183"
     CDS             2926..3534
                     /locus_tag="IXG44_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HAQ1658886.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3859..4182
                     /locus_tag="IXG44_002184"
     CDS             3859..4182
                     /locus_tag="IXG44_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAQ1658887.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4364..5734
                     /locus_tag="IXG44_002185"
     CDS             4364..5734
                     /locus_tag="IXG44_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HAQ1658888.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            6081..>7187
                     /locus_tag="IXG44_002186"
     CDS             6081..>7187
                     /locus_tag="IXG44_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAQ1658889.1"
                     /translation="MPYILALDVSMGHSYSVLYKDDTCLFEDEIEHTQRGFHALLEEI
                     QHLPERPLIVFEATGIYSRPVEKFFQDHHFSYCLLNPLEAKKQMEESTLRSWKTDKSD
                     AHRLAQTHLKNQRQPKEVQSNFYLEMRDLARFYQEIEKKITRLRMDLHNCLQLTFPEL
                     EQFFSNRLTPYALTLIRLFPHPDFVLASTRTKIKNKLINETRKKISANRAEQKADQII
                     HYAQCAYPAVEKDSIHCQKTIYYAELLQDLLEQKKALATQMIKKAEGSPCFLLYQTFP
                     GIGALTAALLLGELGDITRFKTHKQLNAFVGIDIRRYHSGKYTGQDRINKRGNPKARK
                     IIFFTIRNMIRQQRAAPNHIVDYYYKLKKQPIPKK"
BASE COUNT         2110 a         1445 c         1343 g         2289 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaac agttggctgg tgactgttca aaaagcagaa tccttgcttt
      421 tcaaacggtc gcagtccgtt caactgtttc gctgcgcact atttatggtt taagaaacta
      481 gctacttttg ttgtaatcaa atcaatagca acgtggtttt ctccgccttc tggaacaatg
      541 atatccgcat agcgcttcgt tggttcgata aattgatgat acatcggttt aacaactgtc
      601 aaatattgtt cgatcacgga gtccaatgtg cgtccccgtt cttccatatc ccgtttgata
      661 cgacgaatga tacgaatgtc atcatctgta tctacgtata ctttgatatc catcaagtca
      721 cgcagacgtt cgtcttctaa aatcaggatt ccttctagaa tgatcacttc ttttggttcc
      781 tgaatgatcg ttgcctgact tcttgtatgc gctacatagt catacacggg tttttcaatg
      841 gctttatagt tcagtaagtc gcctacatgc tgaatcagca gatcgttatc aaaggcaaat
      901 gggtgatcgt aatttgtgtt caatcgttct tcaaaactca aatggctctg atccttgtaa
      961 taagaatctt gttcaagcat catgatggaa tggtccggga agttgttgaa aatcgcacgg
     1021 ctgacacttg tctttccgct tccggatcca cccgtcacac caatgataat tggcttgctt
     1081 tttgtcattg actaaaacct ctttcgtatc cgttttttca ttctaatcta ttatagcgaa
     1141 aaaacacgaa aatgctatat ttttctgaaa attaatcctt ttcaatttcc aataatgaat
     1201 aaagttcctc taatgaatcg attcgataag taggctggat ttcttcgatt tctggtaatt
     1261 tatttctatt gaaccaaatc gtatcgatcc ctgccatatt tcctcctttg atatcagaag
     1321 tcaaagaatc accaatgatc acagtttgct ttttatcaaa attaggaatg tgggaaaaga
     1381 catgatcaaa aaattctttc atcggctttt gcgctccgac agcttctgag acaaagatat
     1441 ccttgaagta atcaagcatt cctgcatctg ttaatcgccg atgttgcgtg tgcgacacac
     1501 cgttcgtcac aatataaaga tcagcatgcg gatggacctt tttcaggatt tctagacttc
     1561 ctggaacaag atcatgacct tggttcaagt agtggcggta gcgtttttcc atttgatcac
     1621 tatctactgt tttcccaaat tgctggaaaa gtaacccaaa acgactgcct attatttctt
     1681 ctcgtgatag tctgcctttt tcaaactcct gccataaatg atgattcaaa gccttatagc
     1741 tggtttcgat ttctggtgtc aatgtgattc cttgttctgc aaacaaagca cttaaagcat
     1801 tggcttcagt taattgaaaa tctaacaatg tatcatctac atcgaacaat aaagttttat
     1861 atttcatctt tttgacctcc tatttatata aaatacttaa ctttttcatc tctgctaatg
     1921 tcaacgatcg atactcgcct actcctagct gttcatctaa ttccaatggt cccatgctta
     1981 gccgtttcaa tgcggtcact ttctttcctt gggctaagaa catctttttg acttgatgga
     2041 attttccttc ttggatcgtc aataaaaccg tactttggtg cttgtctgct ttgagtattt
     2101 ttaaatcagc cggcttacat tgatacccat cagcgaatac gattccattt tcaaatgctg
     2161 ttacgtctgc cggtgtcacc ctttcgttca caactacttg ataagtcttt gaaacctttt
     2221 tttctggaag tagcatttca tatcccaatt gtccattcga agtgatcagc aatagtcctg
     2281 ttgtatctct atctagtctt cccactggat ataatggttg gaaacgatcg gcacttgcaa
     2341 tcagatcgat tactgtcgtc ttttctggat ctgatacagc tgtaacagct ttttcaggtt
     2401 tattgagaag ataataggac tcatttgttt ctaagtgttc acctgctact tcgatttgat
     2461 gcagtaaact atctacattt cgattttgtc gaaattcgac tgtgccatct atcatcactt
     2521 gcttcaaagc aaacagccgc ttcatttcct tgcgtgtcgt ttctaatttt tcttctatta
     2581 atttatctaa acgcatgctg tttcctttct tattactact aattaattat accttgaata
     2641 ttcttgcttg caaatacatc atcatagtta ttccttgact taattaacag ttcacgttac
     2701 ggttaaatag taaagtgaat tggatttctc atacacagcg taatgacctg gtggagaaaa
     2761 gttgaatcct gactttctaa ggggagatgc gcgcatctct ccttttgcgc ttttttaagc
     2821 gccctgcctt cgatgatgtg cacgagatta gcctttagaa ttcacttaaa taaaatctgt
     2881 ccgttaccaa agcagacagt cacattttaa ggaggaattt tggttatgtc agtatcatta
     2941 gaagtaagta aaagagaagt acgtccccgt tcattaagaa acaaacttcg tcacgaaggg
     3001 aaagttccag ctgttgttta tgggtataaa gtagaaagta cccctatctc attcgatggt
     3061 ttgacactaa gcaaattgtt gcgtgaacat ggagcgaaca cagtcatcac gatggacatc
     3121 gatggacaaa aagtcaatac attggttcac aagatccaaa cgaacacttt taccaatcat
     3181 ttcgaacatg cagaatttct atctgtaaac atgtcggaag aaacagaagt cgaaacagaa
     3241 gtcgttcttg ttggcgaatc agtgggtgct aaatctggtg gagtgttagc tcaaaacttg
     3301 tatacagtag tcgtatctgc tacaccagac aaattgcctg aacgaattga agttgacgtg
     3361 acaaacttgg aattaggtga ttctattaca gtagccgatc ttccaaaaaa cgacgattac
     3421 aaattcgtga ccgacagtga agagcaaatc gctgctgtag tcgctcaaag tgaagaagaa
     3481 gaaagcacag gtgaaggtgg cgaaccagaa gtgatcggtt caacagaaga ataatactga
     3541 aacaatcagg tggtgttaga agcggcgggt cagcagcttt taacacgcct tttttatttc
     3601 taatcgttta ttttcatatg gggcttccac ttttgtaaat ggctttacaa acgaaaaatc
     3661 ctaatttacc aagcttttcc tcgcatactt ttatcgtacc tttataaaaa tttatacttt
     3721 gaaaaaaagt attcaattat tgttgcattt ttcctgattc tttattagaa tgaaccctcg
     3781 ttgaagtaag tatttctagt ttatcctagg attaattata aaaataaaac attcgttgat
     3841 tcgaaggagg ttaaacagat gtttgaaagt tttgatagca gccgcaaccg ttacgcttca
     3901 gttggtgtcg tatcaagttt gccagatgaa ttgattgaca gtatatggtt tattattgac
     3961 ctagatctta agggagtcat tccattagaa aatatattaa cctttgattt gatcgaaaat
     4021 cgtggaaaag tgacgatgca tttttctcaa gcaggaagca atgtggaaat ggatattgat
     4081 ttgccgtttg cctattcgaa ccaatttcct gctcaagtat acgcctatga tgacggtacg
     4141 agagaaacga tccttttacc aagtgagatt agacaatatt aaggtgatag ataaacgtgg
     4201 atgtccctta ttttacagac caggccaagt tgttgattct ttgactcaat tcttggcttc
     4261 tttttatgga gtttcataaa ttatcattga tttctaattg gtattctcag gataaacgct
     4321 atatactact aactaaattt taaagatata aggagtttaa aagatgcgcg atttaacaaa
     4381 aggaagcccc gctaaactga ttttgatgtt cactgttcct ttactgatcg gaaatgtttt
     4441 tcagcagttc tacaatatga tcgatatgat catcgtgggt cagacgctag gtaaaaatgc
     4501 gctggcagcg gttggtgcaa ctggaagcct gacatttttg atcattggct ttgcccaagg
     4561 tttaacagct ggtttagcca ttattaccgc acaacgttac ggtgcaaaag attatcgggg
     4621 tctgaagaaa agttttgctg ctagtgttgt cattagtctt gtcgtcactg tgattttaac
     4681 tgtcctaagt ctcctgttta ttcgtccgat gcttcaattg atgcaaactc ctcctgaaat
     4741 acttgatcaa gcacaaacat ttatttcaat tattttatta gggatctttg catcagtcag
     4801 tttcaatttg ctttctaatg tcattcgcgc acttggtgac agccgcacac cactattctt
     4861 tctgatcatt gcggtcatca tcaacgtcgt acttgatttg atctttatta tctattttgg
     4921 catgggtgtg gaaggtgcag ctattgctac agtaatcgca caagtttctt ccagcgtact
     4981 gtgtctagtg tatatcaaga aaaaaatgcc tttgttacag ttacgcaaaa aagatttcaa
     5041 atttgaaaaa gaagagtttg ctgtacacct aaatgctgct ttgccaatgg cgtttcaatc
     5101 ttcgatcatc gctgttggtg ctatcgtgtt acaagcagca ctaaatagtc tagggacaga
     5161 tgtcgtagct gctcaagccg ctgctggtcg tatcgaccaa tttgcaaatc agccgatgat
     5221 gtcatttggt attgcaatgg caactttcac tgcacaaaac tatggtgcga aagaatatgg
     5281 ccgaatcttg caaggggtac gtcaaacttt actgatgagt gtcggattta gtctcttagc
     5341 aggtgctatc gtcattttct tcggtcattt ctttgttcaa ttgtttgtta gcccatcaga
     5401 aacacgtgta tttgaattag cacaaaccta cttcaatatc aatgggtctc tttactggat
     5461 cttagcagtc ctgttcattt tacgctacac acttcaaggg cttggacaaa gcaagatccc
     5521 tacgatcgcc ggtatgatgg aattgcttat gcgttctttt gcagctatcg ttcttacagg
     5581 aatgtgggga tacccaggag cagcagcagc ttctccttta gcttggatcg gttctgttgc
     5641 cgtgttgctt tactcttata tgcgttcgat gaaacaattg aaacacatgg gtgaagagga
     5701 acatttcaca ttagaacatg cagaagcaag atagccttga ttaaaagaaa tcgttgcggc
     5761 taaaaatgaa aacttttatt gaaaatatct tcttcgttta ccgaaaacag gttttttaga
     5821 taacaaacat actaagatta gaggggaaca tcattgacga atgatgttct cctctatttt
     5881 ctatttttag attatagtga agagtgaaag aaagttcgaa caaagccttg aggactacga
     5941 gtccctatca aaaaccgaac ttctttactg tcattttaga atcaggttta cgtatgttgg
     6001 ggccatgagc ccgtgtatag gaaattggaa tcgaaatgaa gcagtaaagt tctttcaaaa
     6061 cttaaaaagg aggaaaacaa atgccttaca ttcttgcatt agatgtttca atgggacaca
     6121 gctacagtgt cctatacaaa gacgacacct gcttatttga agacgagatt gaacacactc
     6181 aaagaggatt tcatgcatta ctcgaagaga tccagcactt gcctgagcgt ccattgattg
     6241 tttttgaagc aacggggatt tattccagac cagtcgaaaa gttctttcaa gatcatcact
     6301 tttcttactg tttgttaaac ccgttagagg cgaaaaaaca gatggaagaa agcaccttac
     6361 gcagttggaa aacagataaa tcggatgccc accggctcgc acagactcat cttaaaaatc
     6421 aacgtcagcc aaaagaagta caaagcaatt tttacttaga gatgcgcgat ttagcccgtt
     6481 tttatcaaga aattgagaag aaaatcactc gtctgcgcat ggatttacac aactgccttc
     6541 aattgacttt tccagagctt gaacagttct tttcaaacag gctgacccct tacgcattga
     6601 cattgatccg tctttttccc catccagatt ttgtattggc atctactcgg acaaaaataa
     6661 aaaataaact gatcaacgaa acacggaaaa aaatctccgc gaatcgtgcc gagcaaaagg
     6721 cagatcaaat catccactat gcacagtgtg cctatccagc tgttgaaaaa gacagtatcc
     6781 actgtcaaaa aacgatctat tacgccgaac ttctccaaga tttactcgaa caaaaaaaag
     6841 cactggcgac acaaatgatc aaaaaagccg aaggctctcc ttgctttttg ttgtaccaaa
     6901 cgttccctgg gattggcgca ctcacagccg ctttattgct cggtgaatta ggagatatca
     6961 cacgatttaa aacccataaa caattgaatg cctttgtcgg aatcgacatc cgacggtatc
     7021 attcaggtaa gtatactggc caagaccgca tcaataagcg tggtaatccc aaagcacgga
     7081 aaattatttt cttcaccatt cgaaatatga ttcgccagca acgcgcagct cctaatcata
     7141 ttgtcgatta ttactacaaa ttaaaaaagc aacctatccc taagaaa
//
LOCUS       DACEUZ010000119         6913 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.119, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000119 DACEUZ010000000
VERSION     DACEUZ010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6913
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.119"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(208..1899)
                     /locus_tag="IXG44_002187"
     CDS             complement(208..1899)
                     /locus_tag="IXG44_002187"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HAQ1658890.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(2319..3266)
                     /gene="arcC"
                     /locus_tag="IXG44_002188"
     CDS             complement(2319..3266)
                     /gene="arcC"
                     /locus_tag="IXG44_002188"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HAQ1658891.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(3381..4400)
                     /gene="argF"
                     /locus_tag="IXG44_002189"
     CDS             complement(3381..4400)
                     /gene="argF"
                     /locus_tag="IXG44_002189"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HAQ1658892.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(4491..5720)
                     /gene="arcA"
                     /locus_tag="IXG44_002190"
     CDS             complement(4491..5720)
                     /gene="arcA"
                     /locus_tag="IXG44_002190"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HAQ1658893.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
     gene            6181..6882
                     /locus_tag="IXG44_002191"
     CDS             6181..6882
                     /locus_tag="IXG44_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAQ1658894.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
BASE COUNT         1954 a         1422 c         1184 g         2353 t
ORIGIN      
        1 gaaaaaaaaa gggagctgtg acacgacttt tgtcacagtc cccttatttg caaacaaaca
       61 gtgtttaaga gacaacctct ccactaaacg gtgttcattc agctgactct cggtatttgc
      121 tcaaaaaacg ataaaatttg aaaagctatt ttctcttttg tttctctaat actcggatca
      181 gaacgctgat ttcacattct ctttttctta catcttatct ggtgcatgaa gtcctagcaa
      241 acgaaggtct tctttcaata atacagtcac tgcataaact agagctaagc gtgcttcttt
      301 ttgttcatca tcagctaaaa tcttcgtatg agcatagtat ttgttgaatg cttgtgctaa
      361 tttgattgca tgtttcgcaa tcactgacgg ttcgtatttt tccccagcag acaagaccgt
      421 ttcagggtat ttttgaacta atttaaccac ttcccagctg gtatcgtcat tcaatgcata
      481 gtctgctgtt tctgaaggga caaatcctgc tttttccaac aggctgactg cacgagcatg
      541 tgtatattgc acataaggtc cagtttctcc ttcaaatcga acaacttctt ccaggttaaa
      601 gtcaaatgta ttcagtcgat cgtttttcaa atcgtggaaa atgactgccc caaccccaac
      661 ttgtttagct acagcatctt tgttttccaa atcagggttt ttctcgctga tttgttcttt
      721 agcagattgg attgcttcat ttaatacttc ttctaataag acgatctttc ctttacgagt
      781 tgataatttt ttaccacctt gcgtgatcaa accaaatgga atatggtgca tgtcgtcaga
      841 ccagtcaaag cccatttcct tcaagacagc tttcaactgt ttaaaatgat agctttgttc
      901 attccctact acatatagag attgtttgaa gtcgtaagta cgtttacggt acaatgcagc
      961 agctaagtca cgtgtaatgt atagtgtcgc accgtctgac ttcttaatca acgctggatt
     1021 caggtcataa gcagataggt caacgatctc tgcaccttta tcctcgttca gcaaatgttt
     1081 ttcttctaaa agtttgacga tttcatccat cttgtcattg tagaaagctt caccgttcaa
     1141 cgaatcgaaa cgtacttcca gcagatcata gattttattg aattctttca tcgattcatc
     1201 acggaaccat tgccataatt gaatagcctc tttatcgcct tcttccagac gtttgaacca
     1261 agcacgagct tcttcattta attctggttc tgtttcagct acttcatgga attggacgta
     1321 taaacgaagc aattcattga ttggttctgc tttaacagct tcttctgtac cccatttttt
     1381 gtaagcgacg atcagttttc caaactgtgt tccccagtca cctaaatggt tgatgcggat
     1441 tggctgataa ccgatttttt ccataatgaa accgatcgaa ttaccgataa ctgtcgaacg
     1501 caaatgtccc attgagattg gtttggcaat attaggagaa gacatgtcga ttggtactgt
     1561 tccttgattc ccgatgttgc tatccccata atgttctttt tctttcacta ctgtttgcaa
     1621 aacttttttg ctgataagtt ctttattcat gaaaaagttc aagtaaggtc cgacaacttc
     1681 gattttttca aagttggcag aatcgatttt ttctgctaaa tctgcagcga tttgctgtgg
     1741 cgcttttcgg taaacttttg ctaatgaaaa ggctggaaat gcaacatctc cgtgttcagc
     1801 tgattttgga ttttctagta attgctcgac ttgttccagt gttaaatcat ctttgacagc
     1861 gtcatagaca gcttttgcga caagatcttt attattcatc tttattcctc ctaaagtgtt
     1921 cccttgattg ttaaacaaaa aaaatctcta gcgacggcat acttagtatg ccatcactag
     1981 agacgagatt tcccgcggta ccactccaat tgtcctaaaa aaggaccagc tccatctgat
     2041 atcggtagac gaccgtctgt ttaaaacaga attctccaag tgcgcttcgt tgtatcttct
     2101 tcattcggct tccaccactt ccgaattcgc tagccaagaa tgatacaagt actctcttgt
     2161 tcaacaaatc ttagtcttat taaactttct tgaattatat cagaaaaaaa aacgaaaggc
     2221 tagtcgtttc ataaaaaaaa aatccagatg gcctaaagga gaagaaatac cacctggata
     2281 gctataaatc atttcgtaaa tacgaaagac ttaacaaatt aatcagctac gataatcgtt
     2341 ccttcttcag aagctaaaag attctcgata ttttctaaag aagtgatgat tgccttagcg
     2401 tttggttttg cttcaacaaa tgcaatagct gcttctactt tcggaagcat gcttcctgga
     2461 gcgaattgct tttcatcaat gtattgtttc atttctgaaa cagtaacagt ttctaatttc
     2521 ttttgatctg gtttttggta attgacatac acattgtcta ctcctgtcaa tacgatcagc
     2581 aaatccgcat cgatgatttc ggctaatttt tctgaagcaa aatctttatc gattacggct
     2641 tctcgtcctt ctaatccagt agctgtttct acaacaggaa tcccaccgcc accaacagag
     2701 actgtgataa ctccttgatc gaccagcgtt tcgatgacac gtgcttcttt aatcgagatg
     2761 ggtttagggg aagcgactac ttttctccat ccacgtccag catcttcttt gaatgtcaca
     2821 gtggagtcag cattcatttg ttctttggct tcttcttccg tgtagaatgg accgactggt
     2881 ttacttggat ttttgaatga aggatcattt tcatccacaa taacttgagt gactaaagaa
     2941 accacatctt tatcgatccc tttttccttc agtacttcgc ccatggcgtt ttgtaaccag
     3001 taaccgatag atccttctgt cattgcaaca catgtatcta aaggcattgc cggtgttttc
     3061 tcagaatcag cagcttgctg ctgaatcaat aggttaccga cttgcggacc atttccgtgg
     3121 gagatgatca attcatctcc ttgttcgata aatttcacta aatactttgc tgtttccatt
     3181 aacgcttctt gttgggcttt tgcacttgca tccgtagaca agatggcatt gccccctaaa
     3241 gcgacaacta cttttcgttt ggccatttca ttcgtccttt cttcagttct accgaatttt
     3301 ctagataaca aaacgagcca gcgttttgtg attttccctc aatgctgact cgttttttcg
     3361 tgagatgaat gtggttatgc ttaaacgcga gggataaaca agttacctaa tgttgctgcc
     3421 atgatcgctt tgattgagtg catacgattt tctgcttggt caaattgacg agcatatttg
     3481 ctgcggaaga cttcatctgt tacttccatt tcagtgatgc caaagcgttc tttcatttgt
     3541 tcaccgtaaa cagtgtttgt atcatggaaa gctggcaagc agtgcaagaa gatcaaacga
     3601 tctgtgttgt gtgttttttc taccatttcc atattgattt ggtaaggttt caacaatttg
     3661 atacgttctt cgaatttatc ttcttcaccc attgataccc atacatcaga ataaaggacg
     3721 tctgctccgt ctacaccttt atcaacatcg tcggtaatca tcaattgtgc accggatttt
     3781 tcagcaaagc cttctgccat tttgacgatt tcttcttcag gttgtaattc ttttggtgca
     3841 acgatacgca tattcgctcc taaaattgca cctgtgacta ataaagagtt agccatattg
     3901 tttcgaccat ctccgcagta agcaacagtg ataccttcta ctgttccaaa gttttcttgg
     3961 atagttaaaa agtcagcgat catttgtgtt ggatgccatt catcagtcaa gccattccaa
     4021 actggaacac cagagaattc tgctagttcc tctaccattt tttgactgaa tccacggaat
     4081 tcgattccgt caaacatacg tcctagtact ttagctgtat cttctgtgga ttctttttta
     4141 cctaattgga tatcatttgc acctagatat tcaggatgtg cacctagatc gatcgctgca
     4201 gtagtaaatg cagaacgagt acgtgtagat gttttttcaa ataaaagcgc aatatttttt
     4261 ccttctagat agtgatgtgg gataccacgt tttttcaagt ctttcaagtg ttctgaaaaa
     4321 tcgatcaaat attgtaattc ttcttttgta aagtcttttt ctgctaataa gcttcttcct
     4381 tggaatactg attctttcat attaaacatt ctcctttaga ttgtttttat ttaagattaa
     4441 agaggaaatc ccgacggcag ctgacagtaa gactgccgtc aggaaacaaa ttacttcaag
     4501 tcttcacgga caagtggttg gctcatgcag cgtggaccac cacgacctcg tgacaattca
     4561 cttgaattga tttctaacac tttgatgcca tagctacgaa gcaattcgtt cgatacataa
     4621 tttcggttat aagtgactac aacacctgga gcgattgcca atgtgtttga tccatcattc
     4681 cattgttctc gaggtgcaac gatctcatct ccattaccag ttgggatcag tacaagatca
     4741 tctaatccaa gagcagcttt catcgtttcg tgtaagttat ctgaatgagt aatcttgata
     4801 tcatcaccat ctggttcgat tgtaaagaca tccatcttac cgtctttact ttgaattgct
     4861 ggatggatag tgaatttatc gtgatctacc attgtaaata cggtatccaa atgcatcatt
     4921 gcacggttgt tcggaatttt gattgccaac actttttcaa atcctgaatt ttttgcgaat
     4981 aggtggcgtg ctaatttttc taaagctttc gcattagttc gttgagaaat cccaacagct
     5041 actactttat cgcttaagat caattcgtct ccgccttcga tatgatctgg attttcacga
     5101 tccaaccata cttcgattcc tttatttgca aatcttggat gatgtttcaa aatatattct
     5161 gtaaacatcg attcacgacg acgtgcttca aaagtcatct tattgactgt catcccattt
     5221 cccattgatg cggaagggtc gcgtgtaaag tacaagtttg gcattgggtc catgtaaaat
     5281 ggatagtcat cgtctgctga cagatcatat agactgctag aacgtacttc gatatctttt
     5341 gtacgaaccc cagccatgat tttatctacc atttcttgcg tttccattga tagaaggaac
     5401 tcatgaagac cctctcttac agcattcgat gcaatatgag attcatccaa cattttattc
     5461 aagaattgtt ctttgacatt gcctgcgtca atcgcttctg ccgctaattt ttccaagtaa
     5521 agagtctcga cgccttcatt ttgcaatgtc ttcgcaaagt tgtcatgttc ttcttgagca
     5581 atcggaagat atggaatatc atcgaacaac agacgatcca taatatctgg tgtcaaattt
     5641 tccacttctt gtccaggccg tttcagtaaa actgtcttta atttccctat ttcagagaaa
     5701 acgtgaatag gtttatccat gttgtgttgc ctccttcttt ttgattacat gtataggata
     5761 tcctcctttt gtaatcactt tcataatttt gtctcattca aaatgagaca ttcctcacat
     5821 taatattcga taattaaata attaaacgtt tttctatttt taagcgtaat ttattcattt
     5881 tttattctgt atacactttc aaaaaaatat ttattcattg aaaaaatttt tttgaataaa
     5941 ttaaaaaaat aaattatatt atcgttgtta gcgtttacta tatgtattat tgttgttttt
     6001 tatacacaaa tcattcgctt caaaacaaat tattcaatac cattgtatat tttttacata
     6061 ataacaaatt atatttgtaa agtattaaaa cttgtttcta atgttggtat gttaaaactt
     6121 tacaaattct ttaaaaaaag tgacaataaa tatagtgaac aataaggaga ggacttttta
     6181 atgcgtgtta ataaaatcga ttttcacttt tcaaaactca gatatcaacc tgattttgaa
     6241 catttctcgg atgaagagtt ccaacagatc aaggaacata ctgttcttcg ttcttataaa
     6301 aaaggacaga tccttttcga cgatggtgat cccagacaac ggttgtatat cttgaccaaa
     6361 ggactcgttc gtttagaacg ttatgatgaa agtgcaactt attactacta tgactatgtg
     6421 aatacaaata acctttttcc tatgtcagga atgtttggag aagagaccta cacttataca
     6481 gctcaagcga tgactgatat tgaaacattc taccttcctg tttcctacta tgaaaaatta
     6541 gcacgaaaaa atcccgaaca gttgatctat atgatacaaa aaatttctaa agtagttgaa
     6601 atacatgaat tgcgtatcca aatcggattg acctctagcg cctttgaccg ggtaaaacaa
     6661 aatttattca ttttgaaaga agaattgggc gtaccaacaa aagatggtca tatcaccatt
     6721 ccttatccga tcaccttaaa agaattagca gtgaatagcg gcacgacacg tgaaactgct
     6781 ggacaagtaa tcaaacaact acgtgattcc gggctgttag attatgacaa aaagcgtttt
     6841 actttctgta agacagctac tactccagct atgagagaat aacgcgaaca aaaataagca
     6901 gtgaccctga taa
//
LOCUS       DACEUZ010000120         6892 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.120, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000120 DACEUZ010000000
VERSION     DACEUZ010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6892
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.120"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            <1..227
                     /locus_tag="IXG44_002192"
     CDS             <1..227
                     /locus_tag="IXG44_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HAQ1658895.1"
                     /translation="KTTEFDILHALMSTPNKVFSRSDLIEKVKGIEFDGLDRSIDSHI
                     KNLRHKIENDAKSPMYILTVHGTGYRFGGH"
     gene            227..574
                     /locus_tag="IXG44_002193"
     CDS             227..574
                     /locus_tag="IXG44_002193"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1658896.1"
                     /translation="MRKRTIKTQLAVSFLAIATLIIGSISLVALSLMNNHFSKYVEER
                     QEDLLNQYVYTIDLLWLNSGETWNSEELAALSEKVLENNIYFSIEDEQGNMVWELTGK
                     DLKSAQEKLKKMH"
     gene            571..1620
                     /locus_tag="IXG44_002194"
     CDS             571..1620
                     /locus_tag="IXG44_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ1658897.1"
                     /translation="MKVSEKNSVKLDETIEVKKKLINDGNEFGKVTFYYFGPFAYTEH
                     DALFISSMKQSLMYVAIAALLVSFILASWISARLGLPLKHVSDFTHKLTRGEYADKIP
                     QETSIIEINSLIDSLNDLSNQLEKQHGLRKRLTTDISHELRTPLATLKGNVEGMIDGV
                     WKITPERLQSCYDEIDRLTRLIGNIEIINKIEAKYDHLNKTEFNIYKLIESVIENFAS
                     KIESKNLHVEIQGDNINISADKDKMNQVVINLLNNAIKFTQKEGTIKFSISKNKDHVL
                     LIVEDNGIGIEKDQQLHIFDRFYMADPSRSRALGGQGIGLAIVKSVIEAHKGSITVKS
                     KLGLGTKFVIKLPFQ"
     gene            1635..1841
                     /locus_tag="IXG44_002195"
     CDS             1635..1841
                     /locus_tag="IXG44_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1658898.1"
                     /translation="MFQKQIKIAKLVCECWAKKIFFQLISIDSVEKVHLDILSNSVVI
                     FSKKDLSIATIKKEVFGLQIYEMT"
     gene            1862..2458
                     /locus_tag="IXG44_002196"
     CDS             1862..2458
                     /locus_tag="IXG44_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhK family protein"
                     /protein_id="HAQ1658899.1"
                     /translation="MKTVKKMTKLVFASLGIIVFLGACSNQSESNNSNSTNEESTSTA
                     SSEMNSMEGMNHEGMVPSSMKDAANPKFPVGSNVILLGDHMEGMKGANAQVVGAFDTT
                     MYEVSYEPKTGGPMVNNHRWVVQEELKDTETVANEGDTVILNADHMDGMMGAEAKVDK
                     SITGTVYVVNYTPTDGQEEVKNHMWVTEDEMEYDENNE"
     gene            3410..4009
                     /locus_tag="IXG44_002197"
     CDS             3410..4009
                     /locus_tag="IXG44_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ1658900.1"
                     /translation="MKSVEPIRDKKKIDAMKAILASGKYGQRNLVLFSIGINTAYRIS
                     DLRQLKLRDVLEISRGRVIVKERLAMKEQKTAKHNSVFISNKLRKVILDYVQSEFPEQ
                     LQAQDFSKYLFPSRKGADTPLTRQSLWRIIHEAGTAVGLKEIGPHSMRKTFGYFLYKQ
                     GTKTEIIQSLLNHSSQRETLRYIGITQEDKDTAVKSLDL"
     gene            4058..4774
                     /locus_tag="IXG44_002198"
     CDS             4058..4774
                     /locus_tag="IXG44_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HAQ1658901.1"
                     /translation="MNEEKQPNFPDKYHLSRKESVYLLKKNIVELVYNAGKFEGLNTT
                     LLQTEGIIKYNRANNVAVDDVLTVVNLKRGFEMLLNDVQEPLLETSKRINRIVAAEDA
                     LFPGEIRTGGVELSTIQGRYVPPMLTEDEVNNQYGEIMNQEISDTEKALRLFLFISKN
                     QIFWNGNVGTALLIANKIMFSNGLGLLSIPENVFMKFNELLSKYCNSNLLVNESNIIS
                     FLYEQCIFGINYLKIIQHTI"
     gene            4788..5567
                     /locus_tag="IXG44_002199"
     CDS             4788..5567
                     /locus_tag="IXG44_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002396205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HAQ1658902.1"
                     /translation="MMTIDEAKQLATYFSVGSAGYILDFTNVTWNIFTKKIVGIEIQN
                     HYGGSKGVSFQKFLLDDQFDDSLKNRLIQSLKGIMDRDKKYESTEMNPIVQLLFEEVF
                     KEDTSTALRKMKFSSEIDLLYIRQLPIRIQTDIKNKDFDSVLTKANTLIDEVLKFIIE
                     NTEGPSVNINLFKSKELRKEVFGRLNIKTEKDMDNRIKALVGALNTLSDKILEMRNSQ
                     GDAHAHGSNRIVIKEEEAILVANSSMVLCEYVFTKYKGLNR"
     gene            5650..6006
                     /locus_tag="IXG44_002200"
     CDS             5650..6006
                     /locus_tag="IXG44_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ1658903.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVDNGRYVITHGFTKKTEKTPITE
                     LKHAKELREEWFTNDD"
     gene            5996..6262
                     /locus_tag="IXG44_002201"
     CDS             5996..6262
                     /locus_tag="IXG44_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1658904.1"
                     /translation="MMIKKLIEEKKKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            6421..6699
                     /locus_tag="IXG44_002202"
     CDS             6421..6699
                     /locus_tag="IXG44_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1658905.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYVNNSSGQSVINKQIVENRVLLQSFKNKISFCIISSF"
     gene            complement(6696..>6892)
                     /locus_tag="IXG44_002203"
     CDS             complement(6696..>6892)
                     /locus_tag="IXG44_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010868911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAQ1658906.1"
                     /translation="NNLIEQDHRSIKRRHKLYQSLRTASTTIKGMETIRGIYKKSRKE
                     GTLFGFSVCTEIRILLGIPA"
BASE COUNT         2534 a          959 c         1335 g         2064 t
ORIGIN      
        1 ttaaaacaac tgaatttgat attctacacg ctctaatgtc cacacctaat aaagtctttt
       61 ctcgtagtga tttgatagag aaagttaagg gaatagaatt tgatggacta gataggagca
      121 tcgattctca tataaaaaac ttaagacata aaattgagaa tgatgctaaa tcacctatgt
      181 atattttgac agtccatggt acaggataca gatttggtgg ccactaatga gaaaaagaac
      241 gataaaaact caattagctg tttccttttt agcaatcgca acattaataa taggatcgat
      301 tagtttagtc gcattaagtt tgatgaacaa tcattttagt aaatacgtgg aagagcggca
      361 agaagatttg ttgaatcagt atgtatatac aattgattta ttgtggttaa atagtgggga
      421 aacatggaat agtgaagaac ttgcagcact atctgagaaa gtattagaga ataatattta
      481 tttttctata gaagacgaac aaggcaacat ggtttgggaa cttactggaa aagatttgaa
      541 gtcagcacag gagaaactga aaaaaatgca ttgaaagttt ctgagaaaaa cagtgtgaaa
      601 ctagacgaaa caatagaagt gaaaaagaag ttaattaatg atggaaacga gtttggaaaa
      661 gtaacgtttt attatttcgg accttttgct tatacagagc acgatgcctt gtttatctcc
      721 agtatgaaac aaagtttaat gtatgttgca attgcagctc ttcttgtatc attcatactt
      781 gcttcgtgga tctctgctag attaggattg ccactaaagc atgtaagcga ttttacacat
      841 aaattaactc gtggggaata tgctgataaa atcccacaag aaacttcaat tattgaaata
      901 aattccttaa ttgattccct aaatgattta agtaatcagt tggagaaaca gcatgggctt
      961 cgaaaaagat taactaccga tatttcacat gaattaagaa ctccattagc tacgcttaaa
     1021 ggcaatgtgg aaggaatgat tgacggagtc tggaaaataa ctcccgagcg cctacaatct
     1081 tgttatgacg aaatagatcg tctaactcgt ttaattggaa atatagaaat aatcaacaaa
     1141 attgaagcaa aatatgatca tttaaataaa actgagttta acatatataa gttgattgaa
     1201 tccgttattg aaaatttcgc cagcaaaatt gagtcaaaaa accttcatgt tgaaatccaa
     1261 ggggataaca tcaatatatc tgcagataaa gataaaatga accaagttgt tatcaatctt
     1321 ttgaataatg ctattaaatt tacgcaaaag gagggaacca ttaagttttc tataagtaaa
     1381 aataaagacc atgttctttt aattgttgaa gacaacggca tcggtataga aaaagatcag
     1441 caacttcata tttttgatag attttatatg gctgacccct ccagaagtag agctttaggg
     1501 gggcaaggta tcggattagc tattgtaaag agcgttattg aagcgcataa aggtagtata
     1561 actgtaaaaa gtaaattagg tctaggaact aaatttgtta taaagttacc atttcaataa
     1621 aggaggagag ttatttgttt caaaaacaga taaaaattgc taaactagta tgtgaatgtt
     1681 gggcaaaaaa aatatttttt caactgattt cgatagatag tgtcgaaaaa gttcatttag
     1741 atatcttatc aaattctgta gtaatctttt ctaagaagga cttatcaata gctacaatta
     1801 aaaaagaagt ttttggatta caaatttatg agatgaccta aatgtgatta attaaaggag
     1861 gatgaagaca gtgaaaaaga tgacaaaatt agtatttgca agtcttggaa ttattgtatt
     1921 tttaggagcg tgtagcaatc aaagtgaatc gaataattct aatagcacaa acgaggaatc
     1981 gacttctaca gcgtctagtg aaatgaatag tatggaggga atgaatcacg aaggtatggt
     2041 tccttcttca atgaaagatg ctgcgaaccc taaattcccg gtgggaagta atgttatact
     2101 gctaggcgat catatggaag ggatgaaagg tgcaaatgct caagttgtag gggcctttga
     2161 tactacaatg tatgaagtaa gctatgagcc aaaaactggg ggaccaatgg ttaacaatca
     2221 tcgatgggta gtacaagaag agttaaaaga tactgagact gtagcaaatg agggcgatac
     2281 tgttatcttg aatgccgatc acatggatgg gatgatggga gctgaagcaa aagtagataa
     2341 atctattaca ggaactgttt acgttgttaa ttacactcca accgatggtc aagaagaagt
     2401 taagaatcat atgtgggtaa ctgaagatga gatggaatat gacgaaaata atgagtaatt
     2461 ttatatacta aaccaacaag ctggtacatg gtcttcgtat tctcaacatg aggaatatga
     2521 gctctttgtt aataaggact gatgagttgt gcaaaatcaa atctgggtca gaacgaacct
     2581 cattctggct cagatttttg gctcttcgac atttagtgct ggtagagaga caactgaaaa
     2641 tttagtcata accagttagg cagagtgctt aactggtttt tgttcgcatt tattattctt
     2701 ttgatattgg ctgcttgccc ttcaatttta tttggtttct taatcaactc ttcatataac
     2761 cccaaatcat atattaatga ggtgcctcct attcctataa atctatctct tctttaatag
     2821 tatgagaaaa gtctactctt gtagatttaa attgtcaaca aaaaatttac agatgtaaat
     2881 gataagttga atagagaaag ataaattatt tttttggtat tcatataagg ttaattatgt
     2941 attatcacaa aatgattgtt gtcgaataac taaaagtgtc aaaacaatga tgaaatcatt
     3001 tagttcttat aaattagaca agagaaaata aatattccat atctacttta ttggatccat
     3061 aacatgacta aattctgaat actttttaaa gcttatcatt tggtaaaact gaatataagt
     3121 taattatata gactgaaaat tttgaatttt cagaatagaa taacttaaaa gtactgacaa
     3181 attttttaaa ggggcaatat taatgttctg agataatcaa atcttaccga aaatatcaaa
     3241 agtccaatgt gacacaattg gattttgtca cattcgttaa aatcgctcca tccttagagg
     3301 ctctaatagg aaatcacttt tcttttagcg ccttttttct atagcttgtt taaatgtgac
     3361 ttattatctg ttataatcgt attaagtaac atttgagagg tgtctaaaaa tgaaaagcgt
     3421 ggaaccgatt cgcgacaaaa aaaagatcga tgccatgaag gccattttgg cttccggaaa
     3481 atatgggcaa cgaaatttgg tgcttttttc gatcgggatc aatacagcct atcgaatctc
     3541 cgatttaaga cagttaaaat taagggatgt gctagaaatt tcccgtgggc gggtgatcgt
     3601 caaagaacgt ctggccatga aggaacaaaa aacggccaaa cacaattcgg tcttcatttc
     3661 gaacaaattg cgaaaagtga ttcttgatta tgttcaatcc gaatttcctg agcagttgca
     3721 agcacaagac ttcagcaagt atttgtttcc aagtcgaaaa ggagcagata cgcctttgac
     3781 ccgacaaagc ctttggcgga tcattcatga agcaggaact gcagttgggt taaaagaaat
     3841 tggtccccat tcgatgcgta agacctttgg ctatttttta tacaagcaag gaaccaaaac
     3901 agagatcatt cagtcgctgc taaaccattc ttcacaacgg gagaccttac gctatattgg
     3961 aatcacccag gaagacaaag atactgcagt aaagagtttg gatttatgac agaataatgc
     4021 cctatataat agaaggaaaa attcaagagg tgagaaaatg aacgaagaaa agcagcctaa
     4081 ctttcctgat aaatatcact tatctcgcaa agagtctgtg tatttattga agaaaaacat
     4141 tgtagaactg gtgtataatg cggggaaatt tgaagggtta aatacaacgt tgttacaaac
     4201 agaagggatt attaagtata atcgggccaa caacgtggct gtagatgatg tcctgaccgt
     4261 cgttaactta aaaagaggct ttgaaatgct tttaaatgac gtacaggagc ccctgttaga
     4321 gacaagtaag cggattaatc ggatcgttgc agcagaggat gcgctttttc ccggtgaaat
     4381 acgaaccggt ggggtagaat tatcgacgat tcaaggaaga tatgtgccac caatgttaac
     4441 tgaagacgag gtaaacaatc aatatggtga gataatgaat caagagatat cagatacgga
     4501 aaaagccctg cgactgtttc tattcatttc gaaaaatcaa atattctgga atggtaatgt
     4561 gggtacagcc ttgttgattg cgaataagat tatgtttagc aacggtttag gactattatc
     4621 aatacccgaa aatgtattta tgaaatttaa cgaactttta tcaaagtatt gcaacagtaa
     4681 tctattagtc aatgagtcaa atattatatc atttttatat gaacagtgta tctttggtat
     4741 taattatttg aaaataatcc aacatacaat ttaagaggta taagtaaatg atgactatag
     4801 acgaagcaaa acaattagca acttattttt cagttggttc agcgggatat attttagact
     4861 ttactaatgt aacgtggaac atatttacaa agaaaatcgt agggattgaa atacaaaatc
     4921 attatggggg ctcaaaggga gtatcctttc aaaagtttct gcttgacgat caatttgatg
     4981 attcgttaaa gaatcggtta atccaatctt taaaaggtat tatggatcga gataaaaaat
     5041 atgaatctac tgaaatgaat cccatagttc agttgctttt tgaggaagta tttaaagaag
     5101 atacttcaac tgcattaaga aaaatgaaat tttcaagcga gattgattta ctttatattc
     5161 ggcagctacc cattaggatt caaacagata ttaaaaacaa ggattttgac tctgttttga
     5221 caaaagcaaa tactttaata gatgaagttc taaaatttat tattgaaaat accgaaggac
     5281 cgagtgttaa tattaattta tttaagtcaa aagaattaag aaaagaagta tttggccgcc
     5341 tcaatattaa aactgaaaaa gatatggata atagaatcaa agcccttgtt ggagcactca
     5401 atacattatc agataaaata ctcgaaatga gaaatagtca aggagatgct catgcacatg
     5461 gctcaaatag aatagtaatt aaggaagaag aagcaattct tgttgcaaat tcttctatgg
     5521 ttttgtgcga gtatgtgttt actaagtata aaggattaaa tagataataa aatgcatatt
     5581 ttatagtata caattcgaat ttttatgtta tatactataa tataaaatca atatatgata
     5641 taattgggta tggaaaaaat atcttttgaa acatttaaaa gacccaatgg tcatgatgaa
     5701 tttttagaat ggttggaaac attaccaaaa aaagatagtg cgaagctttt acgtacaata
     5761 gaagaaacag aaaagaatgg gatgctagta gctcaacggt tgaaatgggt gaaaaaatta
     5821 gacactaatt tgtacgaatt aagaagtaag gtaggatcaa acatccaaag agccatctat
     5881 ttccatgtgg acaatggaag gtatgtcatt actcacggat ttactaaaaa aacggagaaa
     5941 acaccaataa ctgaactcaa gcatgcaaaa gaacttagag aggagtggtt cacaaatgat
     6001 gattaaaaaa ttgattgaag aaaagaaaaa agataaagat tttgcagaag catatgaccg
     6061 tgaaggaaga aaactcgaaa ctgcggttgc tctttatcat gctcgggagg aagcagggct
     6121 aactcaagcg gagcttgcac aaaaagcaca tacaactcaa tctactattg ctcgaattga
     6181 gagaggggat aacgtatctt tcgataaact tgatgatatc gccagggcat taggtaaaaa
     6241 gctggtagta gaatttgctt aaaagcgagt tgaaaatata tctataatag gctcctaact
     6301 aatcaattag tttagtgggc ctttttttac tagatagata aaggttgtac tatagtgtaa
     6361 aagacaataa attttatact aaaattactt taaagagaac tagagagaaa ggtacaataa
     6421 atgaaaacca taaatcttat aaaagtattt atagcatcac caggtgatat agcagcaaaa
     6481 aggaatgaag tagaagaaac catatataga tggaatatag aaaatacaga taatacaaaa
     6541 gtgattttga tgcctatacg atgggaaact aatgcgattc ctacttatgt aaataatagc
     6601 tctgggcaaa gtgttattaa taaacaaatt gtagaaaata gggttctgct gcaaagtttt
     6661 aaaaataaaa tcagtttttg tattatttct tcattttaag ctggaattcc caataatatc
     6721 ctgatttcag tgcagactga aaagccgaag agagtgcctt cttttcggct tttcttgtag
     6781 attcctcgaa tggtttccat ccctttaatt gtggtggaag ctgtacgtag actttgatac
     6841 aatttatgtc gacgttttat cgatcgatga tcttgttcta ttaaattgtt aa
//
LOCUS       DACEUZ010000121         6881 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.121, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000121 DACEUZ010000000
VERSION     DACEUZ010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6881
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.121"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(1527..2192)
                     /locus_tag="IXG44_002204"
     CDS             complement(1527..2192)
                     /locus_tag="IXG44_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1658907.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDF
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            2318..4354
                     /locus_tag="IXG44_002205"
     CDS             2318..4354
                     /locus_tag="IXG44_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HAQ1658908.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVSINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERVISLF
                     TDRFNQAFHTDYATNIFEDFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLL
                     MNKEISLLLNNDFSLHQVLWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYV
                     FLFSGIYGISLISLLLVEKIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFS
                     SSQLAVSHEPPKEIAGEDYHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSI
                     LVNVSDYLNEENEQFGRGIKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRH
                     TDLKNDLAQITEYYQEISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSM
                     YLERNILSGEIYPPLKIKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLS
                     RSFHYLIQIFLLTFFSFFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNL
                     IKWGTTSVIFLFLIEREPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            4358..4996
                     /locus_tag="IXG44_002206"
     CDS             4358..4996
                     /locus_tag="IXG44_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1658909.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            5024..5452
                     /locus_tag="IXG44_002207"
     CDS             5024..5452
                     /locus_tag="IXG44_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1658910.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            5909..6820
                     /locus_tag="IXG44_002208"
     CDS             5909..6820
                     /locus_tag="IXG44_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ1658911.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
BASE COUNT         2366 a         1040 c         1260 g         2215 t
ORIGIN      
        1 taaatggaat catttcaggt aatctcaaat aaaaaaatat acagtcctgt ggcactttac
       61 ctatgggact gtatattttt tactctcagc gggcttgttt tgactccgtg tctgactccg
      121 tcgtattgat tttaaatgat attcgatgac catttaacag gtcatttttg aaatttttgt
      181 ctctatatga ctcagtttga taattattga tagatcaatt tgggtctata tcgaacaagg
      241 gaataatcca ataaaaaatg atttagaata tcaaaataac aaaaaagagg actacctgta
      301 ttcgtcctct tttttgacca gattttgacc agtctaattt acattctaat atattcatat
      361 gagctaattt tttgattaaa tggtaagttt ttccttgttt gttttagttt aaactagtgt
      421 gtaatttaaa aatctatatg gaagggtctg tggggtaata cttaaataaa ggctaggaac
      481 ctgataacat caaggttcct agccttttta taataaaatg aagcgaaaat aatttctttt
      541 aaagagccga gattatcagc tatataagag tgaatttgat gcaaaaaaca gagccgaaac
      601 agtcttggtg ttcgtagctc tgcttatatg tttcgaattt aagacaaaga gttggcaaaa
      661 gatgttgata aggaagataa gataactgtg ttatataaac taggtaacta actgtaacaa
      721 gctaatttta agagattctt attagaagac gtttaggaat ataactaaga aaatgattat
      781 acaggtaatt gtcatttcaa taatgttttc taattttcgc gtctttgttt taagaaaaag
      841 gccagaatca gttggaaaaa agttcgtacg atagtgaaga ggggcaaaat ttcctactaa
      901 tattcccact aacaagagat aaaacggcca ggttggtgtg attcctctat aaaaaaatgc
      961 ccatgctacc gaaatcccat agattaacaa ctgaacaatt tttacaatga aaaaattgtt
     1021 ctgccattac tcaaagtctc ttcctccttg tatcgtttat ttgtttcatt ttatcattat
     1081 gataggcgct tgaacaatcc attttgtatt ttattgagta ctcttggata aactaccagc
     1141 gattatcata tttatttatg ttactgtaag ccatcatata aaagtaaatg agcagatcat
     1201 agaaatataa aaaagaacta aaattgatct aaattatgtg cagcttgact acgtaccaat
     1261 ctatattact tactagattg gtcgttaatc tattcggatc gaatgagcaa aatatttttg
     1321 tgaatgtcca tccttgatta tgattccaaa aagaatcatt gggaggctgt ggcgtaaata
     1381 ttatactata agccagaaat acctatcatt ctgaactttt ttatttagga actttcatct
     1441 caaggctttt tcaattaatt tgtacccctg ttttttggat aagatcatgt gcattgattt
     1501 tttctatctc tacgttcatc tcctacttaa tgcctatctg gctcctgagg gcttcttttt
     1561 aaggcgtcgt catagtcttg gctgaggatt tcttggtaat ctgagggaat ggcttcccaa
     1621 gtgagcaggc gaacgttcat gacgtgtttt cctttgtgtt tgattacagc atggtttttc
     1681 tctttttcta cctctccagc gtgaaaagac agccatgtac catggccaaa gatatcatta
     1741 catacccaac gttcgatgga attgtccacg tatcctacag ggagtgtggt atagccgatt
     1801 tgtggtttca aaaaaggatt gaatcgatcg atcgtttcat ttttgtattg ataattgata
     1861 agaaaaagat agaccaaaca aaagacgagc agaaagaaag atagtgtctt tttgagtgag
     1921 aagggttgtt ttgatgaata agatttcttg ttaacaagag gttcatctgg caatgttggt
     1981 tctattgatt cggtagctga ttcaatagaa ggaaaatcaa tcggcagacc taaagtatga
     2041 tgtaaaagtg acagcatttc aaagtctggc attgtacgag aattttccca actagaaatc
     2101 gttttatcgg aaacaccaat tttttcgcct aattcttttt gcgtaagctt acaatttctg
     2161 cggtaagttc gtattttttc tccggtgttc atcttcttct cctttcgatt ctacagtata
     2221 tagatttatt ctacatgacc tatacacttg atagatacgt ttttttgatg aaaaacggta
     2281 tgattttatc aaagaatcat ttaagtaagg gagagtggtg ataatcaaaa aaaagttcac
     2341 agttttactc acaatcatag tgaccggtat catgagtggt tttgccttgg ttagtataaa
     2401 taaacaggta ctctatcgga caaatgaatt tttccatcaa acagatactt atactccgat
     2461 tcgattgcca caacatttta ttgggcaaga agaatatgag cagctaatca tggtatttga
     2521 aaatgtctca tcagaaactg agctttctta tatgaaaaaa acagtaaatg aatccaatga
     2581 agtagataca tcttggaact tcaagataca gccaaagctt gaaatgacat tgtattatca
     2641 aggggatgtc aaatcttctc acgagaaggt accctattct aatacctcct ttcgcgtaac
     2701 gtccattgaa gaatcgatca aaataaaaga ttttgaaggt gaatttttct tgatgacgag
     2761 agaaaaagaa gcctatgaac gggtcatttc actattcact gatcgattca atcaagcatt
     2821 tcacactgat tatgccacta atatttttga agactttgaa aatagctatt cgtattcggt
     2881 tatttcaaat acagacgtat ataattatcg agtgtattta actattggat tgattctttt
     2941 attccttttg ttatcatcct ttttcttatt gatgaataaa gaaatcagtt tattgttgaa
     3001 taatgacttt tcattacatc aagtattgtg gtgtttattg gggaaaaaga aaatcattat
     3061 tacattcata attgcaggaa tcattggtgg ttttattttt atgtattata aggagcaatt
     3121 tctttatttg ttccagatgt acgttttctt gttcagcgga atatatggta tctctttgat
     3181 ttctctttta ttagttgaaa aaatcgcttc gttaagagaa gcgttcaact atatccaata
     3241 cattcttttt acatttggat tgttgttttc tcttcttttg accgttgcca atgtcgaatt
     3301 agcaactatc gttttttctt cttcacagtt agctgtttcc catgaaccac caaaagaaat
     3361 cgctggtgag gactatcatg tattttatcc agtaaccatc gggaaaaacc acgtggattt
     3421 tatttattcc aatcgttttg catcagcagc agatcaggaa ttatatgaaa ctttgaataa
     3481 aaacggaagt atattagtca atgtatcaga ttacttgaat gaagaaaatg aacagtttgg
     3541 cagagggata aaaataaatc tgaattatct taagaaattt cctttgattg atgtctctgg
     3601 aaggttgatc caaataaccg aaaaagaaac gcaccctgtt atattgatcc ctgaacgata
     3661 tagacataca gatttaaaaa atgacctggc gcaaataaca gaatattatc aagaaatcag
     3721 tgaaaaagaa ccgaaatttc tatttataaa aaacaatcaa cccatttata catttattcc
     3781 ttctatcccc tggatcgaac attatccggt tgttgaaata ctgacattga aaaacagtat
     3841 gtatttggaa agaaatattc tcagcggaga aatctatcca ccgttaaaga tcaagatagg
     3901 ctctttgtcg aaagaaaggt tatttgagct gattgaagaa agtcagttac gtgataactt
     3961 gcagttatca tttccgtata ctcagactga agagatagct gtcaaagtgt taagcagatc
     4021 atttcattac ttgatacaga tatttttact cactttcttt tctttcttct tgttgagcta
     4081 tgtcatgtta tgcatatatt ttgtctacaa ttgtcggaaa atcgctatct ttcgttcaag
     4141 tggttattcg ctctttgaaa cgtataaaga tttcttcatg atgaacctca tcaaatgggg
     4201 aacgacaagt gtaatcttcc tttttcttat agaaagagag cctaaatatc tttttaatat
     4261 ttttttcttt agcttcattg attttattct ttcagttgtt tttattttgt ctacagaaaa
     4321 gaaaagtcag ttattgctga tgaatggagg ttaacaattg gagccgttga tagtgataaa
     4381 aggtgcgaaa aaaatcattc gaaacaaaac gattcttcat ttggatcgtt tcactgcgga
     4441 tgctggggat cttgtgactg ttgttggaga aagtggagca ggtaaaagta cgttattgaa
     4501 tatattagga ttgaatgatc agttcagttc aggacaattc tatttgttcg atcaagagac
     4561 gaaacaatta actgggagag agaagcgctt gataaaaaga aataccatat cttttctttt
     4621 tcaagatttt gggttgattg aatatgaaac aatcgatttt aatttagata ttggattgaa
     4681 gttttcgaat ttaaatgttt cagataagcg aacagcaaag tatgaggcat tgaaaaaagt
     4741 aaatataaac aagaagctaa gtacgcctat ctacacatta tctggtggtg agaagcaaag
     4801 aatcgcctgt gcgagagtct tattgaagaa tagcaaaatc attttagccg atgaaccgac
     4861 tgggtcattg gataaaagaa ataaggataa agtagttgaa atcttatcgc aacaagcttc
     4921 ccaaggtacg ttagttatcg tagtcacgca tgatccatat ctgaccaaaa taggaaacaa
     4981 gaagatcatg ctttaaaaat caaaaaggaa aggatgtcgt tttatgaaaa aagcagttat
     5041 tatttgtgca ctagggatag gttttgttgg aggaaacgct atgtcatctg aagtggaagc
     5101 aaattcagtt cacggtgaaa tctattacaa atctttaaag ccaggagaga ttcattttga
     5161 tgctaaaggt aatattatta agtcaaattt accagtagac cgggaaattt ctagaacagc
     5221 aattaatcat agctctggaa gatgggttta ttatagtgat acaacttgta cgagaaaaac
     5281 aggtcattct aaccactata gtcgtgtcta taatcgtcac ggtgcaaaag cgaaagttgg
     5341 aaactctatt cgatcagcaa atgcaacaaa aggagtatgg gcctatgcaa gtgcgacagg
     5401 aaatagaact gacacattct catgttggta caatccaacc ggttggtatt aagtatccaa
     5461 aagtttatat aaaatagaaa aaacatataa tgaaacagcg ttaaaagtta tttttggtag
     5521 ttttaatata ttcattttta gttgtataaa aatatatcat aataaaaaca acaaaaaaat
     5581 gtctggaaaa tcgaacataa aagtgtctga tttgaatatc ccagcagcgg aagaagaaaa
     5641 acaaaatcga taacatttgt tctaagaatt agttacgtta aatacggaac gcaagataga
     5701 cttgaaaaac ggtaacaaaa gaagtagtat attaaggtgt gatgtgctac ttgacattta
     5761 ttctgctgag cgaactttct ggattgtgta gccttatatc tattcaggac gtatcgagca
     5821 gattagtaaa ggtgttcttg atgattttca tcttgaacac ctttgttttt agcaattcgc
     5881 aaaaaacgaa aagtaggaga attgataaat gaaaaaaaga tttttagcac ttgccatcgt
     5941 actaggcact ggcttattgt caggctgtac gaatgccggt gaaaaaacag ccgttagtta
     6001 taaaggcggt acaatcagcg aacaagaagt aatggatagc ttgaaaaaaa tgcaaggagc
     6061 agattccgct gtgcaacagt tgatcgttta tcaagtattt gaagataaat atggagatga
     6121 cgtatcaacc aaagaaatcg attctcaata tgatcaaaca aagaaacaat tgggtgattc
     6181 atttgattca caattgaaat ctgcaggtta cacagaacaa acattcaaag acagtatcaa
     6241 acaaagtcta gcctttcaag aaggtctaaa gaaacatatc aaattgaccg atgaagattt
     6301 aaaaacggct tgggaatctt tccatccaga agtggaagct caaattatcc aagtagcaag
     6361 tgaagatgat gcaaaagatg tgaagaaagc tgctgataag ggagacgatt tctctaaatt
     6421 agccaaagac aagtcaactg atactacgac aaaagaagat ggcggaaaag tgaaatttga
     6481 ttccactact acaacggttc cggcagaagt caaagaagca gcctttaagt tgaaagatgg
     6541 tcaagtttct gatgtgatca cttctaccaa tgcttcaact tatactacgg aatattatgt
     6601 ggttaaaatg gtgaaaaacc aaaacaaagg caacgacatg gataaataca aaaaagaatt
     6661 gaaagaaatt gcaacagaca caaaattaag tgacagtacc ttccagaata aagtaattgg
     6721 cgaagtattg aaagacgcta atgtaaaaat caaagacaaa gactttgaaa atgttttgag
     6781 tacattcact tcagattcta gtacagcaag tagtaaataa gcggtataaa taaaaaatat
     6841 gaactattta aatcaataaa actgttcatc taaatctttt t
//
LOCUS       DACEUZ010000122         6810 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.122, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000122 DACEUZ010000000
VERSION     DACEUZ010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6810
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.122"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(<1..152)
                     /locus_tag="IXG44_002209"
     CDS             complement(<1..152)
                     /locus_tag="IXG44_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HAQ1658912.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEIL"
     gene            complement(165..653)
                     /locus_tag="IXG44_002210"
     CDS             complement(165..653)
                     /locus_tag="IXG44_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HAQ1658913.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            complement(619..1290)
                     /locus_tag="IXG44_002211"
     CDS             complement(619..1290)
                     /locus_tag="IXG44_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HAQ1658914.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            1428..2714
                     /locus_tag="IXG44_002212"
     CDS             1428..2714
                     /locus_tag="IXG44_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HAQ1658915.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            2786..2980
                     /gene="ssrS"
                     /locus_tag="IXG44_002213"
     ncRNA           2786..2980
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="IXG44_002213"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            3058..3342
                     /locus_tag="IXG44_002214"
     CDS             3058..3342
                     /locus_tag="IXG44_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1658916.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            3415..3894
                     /locus_tag="IXG44_002215"
     CDS             3415..3894
                     /locus_tag="IXG44_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ1658917.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            4117..4260
                     /locus_tag="IXG44_002216"
     CDS             4117..4260
                     /locus_tag="IXG44_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HAQ1658918.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            4838..6421
                     /locus_tag="IXG44_002217"
     CDS             4838..6421
                     /locus_tag="IXG44_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ1658919.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
BASE COUNT         2091 a         1404 c         1357 g         1958 t
ORIGIN      
        1 tctaaaatct caccatattg atccgactga aaatgttcaa catctaaaga atcttcaatc
       61 gcgacaccac tcgcccctgc ctccatcaaa atattagcaa ctgcttcgac tgcttcactt
      121 tcagtttcta cttttacttc attccatttc atatctgtca ctccttaata acttgggtac
      181 ggtaaataag tatctgcttc attttgctgt tttacttcta ctaattctgc tagttgctga
      241 ttatcccagt gaagctcgaa tacagcttcg tcatcgtctt catccaaaaa atcaagcaaa
      301 tcgctttgcc cttgagctaa tacttcattt agatattcca ctaaagaatc agctactgat
      361 tttgccaacc ctttttttcc ttcataaggg attactgcta gataattgcg ttcatcaaaa
      421 acagatttct gaggattaaa aagaagtacg ccgtcttcaa attcgataat tggttcttgc
      481 gaaacgatcc cttctgcatc atcaatctca aattgtgctt tattttctgc aaacaagcga
      541 acaacgatct cgatcgtatg attttttgta tcccaatcta aagctacatc atagtctgtc
      601 acttgttttt caattatttc atctaaatat gttaatagat ccttcttttt cattttcttt
      661 cctccaaccc caatctttct cgatttggtt ttttctttgt aaagagatat aaggatttcc
      721 cgacacaaca aaacgcaacg gcttttctgt ccatacacct ttgtttggga taccgattct
      781 aggcaaagca ttgatctttt ttggtgtctt ttttttctca aaaactaagc gtaacgaact
      841 atctgctata gactgaccat aaagttcttg aggaattgct aatgcttcca ctaattttcc
      901 cggaccgttg ctgatatctg gaccagtttt tccgccacga tttttactca tttgatcaat
      961 cattgcggcg ggttcgattg cacgtatcat tactccttga ggaatccctt ctggctgagt
     1021 aatgatgttc aaaatccgat gagtatgcat cgtgtaaagg tagatcgtac ctggtttttc
     1081 atacattgcc cgcacacgcg gtgttcttct taaaccatag ctatgggcgg cctcgtcttc
     1141 tggacctagg tatgcttccg cgtctacaat atatccagct aatcttcctt ctggtgtgtc
     1201 atgctccaaa tacatcccca ataaatcccg tgccacttct ggcgtcgttt tattccaaaa
     1261 aaattcatct atcttttgtt tatccgtcat ttttctcacc tactttatca tacacaatat
     1321 tagttggata agctatgaaa acaaaacaac agaaggctgt tctttctagg catcttgcta
     1381 aatatgatat aattaaggga cgtaaaaaca agaaggagga aaatcctatg caacagccat
     1441 tggcttatcg catgagacct cgaacgatcg atgaagtcgt tggtcagcag catctagtcg
     1501 gggaaggaaa aatcatacgc cgcatggtcg atgctcgcat gctctcttcc atgattctat
     1561 atggacctcc tggtactgga aaaacgagta tcgcaagtgc gatcgccggc tctactaatt
     1621 atgcatttcg catgctaaac gccgctacag attcaaaaaa ggatctacaa gttgtagcgg
     1681 aagaagcaaa aatgagtggt acagtgatcc tcttattgga tgaagtacac cgattagata
     1741 aaacaaaaca ggattttctg cttcctcatt tagaaagtgg ccgaatcatc ttgatcggag
     1801 cgactactga gaatccttat attaccatca atccagcgat ccgcagccgt acacagatct
     1861 ttgaagtcaa accattaaca gaacaagaca tccagttggc agtcgaacat gctttaaggg
     1921 ataaagaacg cggccttgga caacaagcaa tccagttaga cgaagatgct cttcttcacc
     1981 tttcgcgtgc aacgaacggc gatttgcgga gtgcattgaa tgggctggaa ctggctacac
     2041 tatcgactcc ttcagataaa gaaggaagga ttcatcttac actgtcgatc attgaagaat
     2101 gtgtccaaag aaaagctctt acccatgaca aaaatggaga tgctcattat gacgttattt
     2161 cggcttttca aaaatcgatt cgtggaagtg atgtggatgc tgcacttcat tatctcgccc
     2221 gtttagttga ggcaggtgat ctggcaagta tttgccggcg tctgatggtt atcggttatg
     2281 aagatatagg tttaggtaat cctgctgctg cggctcgtac agtgaacgct gtattagctg
     2341 ctgaaaaatt gggattgcct gaggcacgta tcccactagc agacgtcgtc gtcgatcttt
     2401 gcttgtcgcc aaaatccaat tctgcctaca tggcattaga tgcagcactt gctgatattc
     2461 gagagggcaa agctggagat gttcctgatc atttgagaga tagccattac aaaggtgcga
     2521 aatcactcaa ccgtggcgtg ggttatcagt atcctcatca ttttgatcag gcttgggtca
     2581 atcagcaata tttaccagat aaactgaaga atgctcagta ttatcagcca aaagatacag
     2641 ggaaatatga acaggctctg ggtcaacagt attaccgtat aaaagagtgg aaaaagcatc
     2701 ctccaaaaaa ctagacaaga aaacggttct gtttctgcga gctctctcat tgctcttttt
     2761 aaattcatat gctactatag aggtgggaaa tctgtgatgt tcgaattgtc ctctttgtgt
     2821 gttgaccgaa catttttatt gatatcttgg gacccgtctt gtgtcactgc tggtaacatg
     2881 ccttatcatt tggtagttcg aagctacgac catgggaccc acctgcttga attcgcggga
     2941 tcaatactac aggacaaaca acggcattaa cggagattac catcttttaa agaataaagc
     3001 aatttgctct tggcaaattg ctttttctat ttgttgctta ttttggagga tttgttcatg
     3061 attaggattt taatgattat cgccacttta gtattattat tcgttagcta ttatttattt
     3121 aataaacagg atatcttttt tgtattgatc aacaaaaatg ataaaaatca aggattccta
     3181 caattttacg gagctgcata tgctgtttta ggcgtaatgg gtattttaac agcttttttc
     3241 aatcaacgct ttattgcctt ggtcttttta ctgatcgtaa ttattgtttc tgccaccttc
     3301 agtatccgat ttgcaaaaaa aattgctgaa ccaaagcagt aaataattct ctttttccct
     3361 aaagtgttct acaataaaag agtacaatac gattgacttg aggagtgaat gcttatgtta
     3421 caacaataca aaaaaatcat ggttgccgta gatggttctg atgaagctga attagctttt
     3481 aaaaaggcgg tcaatgtagc tatccgaaat aacggagaac ttctattagc acatgtcatt
     3541 gacacacgtt ctttccaaac cgtttcatcc tttgatggaa tgcttgcaga acaagctact
     3601 gaaatggcaa agcaaacatt ggcagattat gaaagcaatg cgaagaaagc tggcttgaat
     3661 aatgtaacct ctgtcgtaga atatggctca ccaaaacaaa ttattgccag agaaatccca
     3721 gaagataatc aagtagattt gattatgtta ggagccacag gattgaatgc tgtagaacgt
     3781 ttatttattg gttctgtttc tgaatatgtt attagaaacg cagcttgcga tgtgcttgtt
     3841 gtccgaactg atttagaaaa ccaattgcca gctaaagacc acaccaaagc ttaacacgta
     3901 gacgacctgc caactatcgg caggtttttt tgcacaaaaa accgtgataa aaacaaaaga
     3961 cgcggcaagc actcatcggt ctttataatg tttaaaataa agggaaaaca gtcaatgagt
     4021 tcaagtaaaa taaaaaaaag tgaacaccag ggcgattagt cgccctggtg tggtttaaaa
     4081 cgacctaagc atgccaaagc ttattggttc gtcaatgtgg aagtttccga tgaatctgat
     4141 acgaaaaaat atgaagtatc gatggcggat accgatttta tcgccaaact aaaattttat
     4201 ttcgggttcg ggtaccgcga gaaggcaagt gctacattcg gtaaatcatt caagaattga
     4261 tgaaaggcta ccgcttgcca ttgcaaccac aacgatactc ctgaacgcga tgttggtgag
     4321 atttccaaag agaagagctg tttaatgcaa cggtattaag caaatggcaa aaggaagtca
     4381 tgtagacaaa gttttttatc aaacaccata tgatttcgcc tgaacgctgg tttagctcag
     4441 aatacaatga tgtggtccac gagacattac cctcagtcag ataaaaaaag ctctctgaca
     4501 gaattacaca tcgataaata ttaagtaaat gtcagcgcta gttgaacttt ctgacacttt
     4561 caggtacact ggaaaagaca actacacgaa ggagggaaaa tactatgatg cgcatgacta
     4621 aaataacaag taatgaaaat taccaacgat accatatcgc atttagccat gcaattggtt
     4681 ttacagttgc tgttgacggg acaagtttgt ccaaaacagt tgaattcacg taaaatcaat
     4741 ggccatctca aggtattttt ctcatgataa tctgagttta aataacacag gccatggata
     4801 accatggcct ttttgagtgg tctcaaggag agagatcatg atcatacaag caatttctat
     4861 taaaaaaaca atcggcggga atccattatt tgaggattta tcctgtcaaa ttgaagctgg
     4921 tgaaaaaatt gccttagtcg gtatcaacgg aactggcaaa tcaacctttt tacaaatctt
     4981 gaccggcaga gaaggtgtcg acagcggtgt tatcagtcgc aaaaaaggct taaagatcgg
     5041 gacggtcgaa caagaactta cagtttccga agcgaccgtc aaccactact tgctccattc
     5101 tgccgctgag atacaggacc tcaaacagca aatgagtcgc tacgaagcat tgatgacaga
     5161 accagataca aacttagaga agtgtctggc ccgttacggt gagctgcagc atcgctttga
     5221 agaactaggc ggttacgtat tagaagaccg gattgcagcg atcttgcaag gcttagggat
     5281 tccacaccgt caggaagcac ggttaacaga acttagcgga ggcgaacgag ttaaagtcgc
     5341 tttagccaaa attttagcag ccgatgccga tttactttta ctcgatgaac cgaccaatca
     5401 tttagatctt aacagtattc gctggttgga aaactattta cagaatacaa aaaaatcggt
     5461 tctggtcgtt tctcatgatc ggcaatttct cgatcaagtc accacaaaaa catgggaact
     5521 ggaagaaggt gcattgattg agtatcccgg aaactacagc cgttttcgag tcttgaaaga
     5581 agctcgcttg gctgagctga caaaaaatta cgaactgcag caaaaagaag tccagcggtt
     5641 gaaagtcatg attcgtcgtt ttcgtcagtg ggcacatgaa ggagacaatg agtccttctt
     5701 caaaaaagcc aaagaactgg aacgccgcct ggcgaaactg acactcgtca aaccgccacc
     5761 tcccccgaaa aatcggctgc aaagcttgtc taatggtgga aaatctggca aagaagtttt
     5821 catcattcaa aacttgcacc agcagtacgc tgatcaagta ctattcaaag acagcagttt
     5881 tgcagtctat cgtggagatc atttagccat catcggcgac aatggtgcag gaaaatcaac
     5941 cttactcaaa ttattactcg gtgaggaaaa gccttacggt ggcaccatca aattaggtag
     6001 tagcttgcaa atcggttatt tgccgcagca gctgcagttt gccgatcccg atagccgttt
     6061 gctggcttac gccatcacat tgactggaaa cgaagaagtc gctcgacggc aattagccaa
     6121 aagtggcttt tatcaaagcg atgtgggtaa acggatcaaa gatttatccg gtggtgaaaa
     6181 aatccgtttg gcgttgctga aactcttttt agaaaaaatc aacgtcctga ttttggatga
     6241 accaaccaat cacctcgaca gctatgctcg agaagaaatt gaagaaatgc tgcaagacta
     6301 tcaaggaacg ctcctagcag tcagccatga ccgctacttt ttgcagcaac acttccaaga
     6361 agcactcgtt attgatcagg agcagatcag ccgccagccg ctcggccaac tgattgacta
     6421 ggacgttttt tcgcaacaaa aaacttgatt ttcatacctg tgctttcacg attcaggcaa
     6481 agtacaggtg gaaaatcaag tttttattat tcgatcaatg aatgtttcgg atcggttttc
     6541 tagctaaaca taaagcgaag aataaagtcg acagcccacg aggctgatct aaaaatgttt
     6601 tttcttttta catatgtaat gtcccttata aagatgaatg aaaataaaat attgggattt
     6661 tcctgaaaat caataaataa aatgccagtc ttttcccttt tgaccacttt tccaagcaaa
     6721 attccctaag aagatcctgt aaaaaagaag ggaaattgaa gttaaattac cattttgcgg
     6781 tcttctccgc aaaaaaaaca catgcaagaa
//
LOCUS       DACEUZ010000123         6610 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.123, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000123 DACEUZ010000000
VERSION     DACEUZ010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6610
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.123"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            810..1484
                     /locus_tag="IXG44_002218"
     CDS             810..1484
                     /locus_tag="IXG44_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAQ1658920.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTVGICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIVYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            1481..1822
                     /locus_tag="IXG44_002219"
     CDS             1481..1822
                     /locus_tag="IXG44_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1658921.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTEEIQKKFAEYLILEAK
                     KRRIKSFFAQF"
     gene            1852..2211
                     /locus_tag="IXG44_002220"
     CDS             1852..2211
                     /locus_tag="IXG44_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HAQ1658922.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            2735..4816
                     /gene="cadA"
                     /locus_tag="IXG44_002221"
     CDS             2735..4816
                     /gene="cadA"
                     /locus_tag="IXG44_002221"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAQ1658923.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQEIKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKIFMQYVASAEQFSTHPIAQSVLEGYAGPLLP
                     TANIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKKAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKNKR"
     gene            5127..6593
                     /locus_tag="IXG44_002222"
     CDS             5127..6593
                     /locus_tag="IXG44_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1658924.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
BASE COUNT         2188 a         1075 c         1410 g         1937 t
ORIGIN      
        1 ggaagaggtt gtgcgcctgc cgttcagctc cgagtcacaa ggagtaattt caaaaaacag
       61 tttttcatgt ttgatgacta ttgtgacttg tgaccgagga gttggccgga gagcaccgtt
      121 tattctaaaa aagaactgcg aactctcgca gttctttttt taaaatacgg gataaaggaa
      181 gtttccagca gacaagagaa gaaagcaaaa gaaattgcta tttgattcac aaggttctat
      241 aacgaaatcg caatcaatca aacatttaca aagaaggtaa ttcaaatgga aatctgattt
      301 gatgatgctt ctgctgttga aataaagcag catctagcac ctgaaaacaa actaatagta
      361 acattcgaag atggagtagg tccgtatttt cagcatgcaa taatccatat gtaagttcaa
      421 ttcacccttg aaatcatcgg accagataaa gttatgacca atctatccca agcaatctag
      481 aagagatatt agtaaaggga catttactag aagtcttaga tgagcctata aatatatgtt
      541 ctgatggcgt catcagtggt ctgattgacg gaagcttagg tgtgatcgat ctctctggac
      601 caaacaacga aggaatcaag gggaatccgg cacgttaaga aaaaaatagc ataaaaaact
      661 agacacttga tgagcgttca ttcaattgtt tgaagggact aggctggtgt cattttttat
      721 acatatattt tctctttcgt cttgataggg cttatgggat aatataagga agaaaatcat
      781 cgttttattc aaaaaggagt ttttgacaaa tgaaaaaacg tatattattt atcggtgata
      841 gcattacaga cgcaggaaga gaccgagcag aagcaactga tctaggtaaa ggttatccac
      901 ttttgattgc cggaaaacta atctcccaat atccaacgat ggaatttgaa tgcatgaacc
      961 gtgggatagg aggagacaaa attgcagatc tgcaagtacg ctggcaagaa gattgtttag
     1021 ctttacaacc tgactatgta tcgattttga tcggaatcaa tgatacctgg cataatacta
     1081 gcgatcaggc tatattcgga actgagaaat ctgctcagca atttgaatcc gtttatcgaa
     1141 aattactaaa tgaacttcaa acagtgggaa tctgcaacat catcttgatg gagccgtttg
     1201 tacttcctat aaccgacgac cgtcatggct ggcgtaaaga cttagaccct aaaatccaaa
     1261 ttgttcgtaa acttgctaag gaatatggcg cattgctggt tcctttagat ggtatattca
     1321 atgctttagg gattgtttat ggtttttcga aatatacaag agaagatggg gtccacccaa
     1381 cgattggcgg acatgagatc attgcacaaa gctggataga tgcaattgcc gcagtcgatt
     1441 tttttagtgg agataaaaat gagcgaaaga aaagtgagaa atgatggaaa tacggtgggc
     1501 agtaaaagaa gatctacctc aaatcaatca gctgattact gatcatccag aatcattcgt
     1561 aaaagcaaaa acggcagctg acctaaatga taagaataaa ctattgctcg ttgctgtaac
     1621 aaaagaaaaa cggattatcg gatttgcgca agcggacgtg caacagtcag aagaacgatt
     1681 ggatcagcga ccatcaggaa aactaatcca acacacatta gtaacaaaag aaaatacgga
     1741 agaaatccaa aaaaaatttg cagaatattt gatactggaa gctaaaaaaa gaagaataaa
     1801 atcatttttt gcccagtttt aatcaaaaac agtaatagaa ggagaaatac aatgagttgt
     1861 ttaggaaatg tcatctggtt tatattcggc ggactaatcg gtggtcttag ctggttagca
     1921 gcggggatac tttggtgtat cacgatcata ggtatcccaa tcggtatgca atgttttaag
     1981 atcgcctccc tttcttttgc gccttttggc aaagaagtaa tttatcacca gtctggcatg
     2041 tcattgattg ttaatatcat ctggctgatc gtttcaggcc taccaattgc tctcgcacat
     2101 gtcgtcagcg gcatccttct atgtatcacg atcataggga taccttttgc caagcaatct
     2161 ttcaaacttg ctcgattggc gttgatgcca ttcggtgcag aaattgtata aaagagaata
     2221 ataaaggtta gataaagcat agagtcaggt gggatacatg tatttcacct gactctatgc
     2281 gttctcctgt ataagttctt ccatgtgact tttacgtctt ttatttatat ggggttactg
     2341 tttatggaaa gttgaaataa ttttcattaa agaactcttt ctatattttt gaaagcgtag
     2401 tggagcttcg aatatctggg aaggacattt tgcagggatt gaaaaaagat attgtcctat
     2461 cgaaaaatta atgaatttag acaaattgcc agcaacaggc ttcaaactta gttgctatcc
     2521 tgtcaagatc aagaaggcaa gtgcgggttg gataagagca gtggcaatga tcgaaaagaa
     2581 aaagaaagaa taagccaaaa catctgtatg gaagaaattt tccgatgcag atgtttttca
     2641 ttttttcttt cggatacctt gacatatgaa taaccgttca tttataatta gttatgaaga
     2701 gatattcata tgttgaggcg aggaggtcat taaaatgaaa agttacagat tagaaggttt
     2761 agattgtgcg aactgtgcaa tgaaaatcga aaaaggagtt cagaagatca atggagtcaa
     2821 ggaagcaacg gtaaacttca cttctggaaa actaacgatt gacgcagagg aagatcattt
     2881 ggctacgatt gaacaagaaa taaaaaaagt agtgaaggaa ttagaaccag atgtcaaagt
     2941 aacagaaata gataaggaaa aagtatctga acatggaaat gaaaaagaga gaaatacgct
     3001 gttccggatt ttattttccc ttgcaggtat cgctttattg ctattatttg attttaatga
     3061 accaattcgc ttgatagggt atttattgat ttatctgctg ataggatatg atgtagtgaa
     3121 gaaggcagtg atgaatatcg tcaaaggaaa aatctttgat gagaattttc ttatgtctgt
     3181 tgctacgatt ggcgctatgt tgatcggtga atatccagaa gccgtagcag tgatgctatt
     3241 ttatcaaatc ggcgagtatt tccaaggatt ggcagtcagt cattcaagaa aatcgattcg
     3301 cgaattgatg gcgatccgtc cagaaacagc tcacgtgcag acgacggaag gattgatgac
     3361 cgtgaaccct gaagacgtgc agatcggtca gtttgtactt gtgaagcctg gtgaacgtgt
     3421 accgttagat ggaacgatca tcgaaggaga atcattagtc gatacgtcag cacttacagg
     3481 agaatcagtc cctaaatctg tatatgttgg tgaaacagta ttaagtggat ttatcaataa
     3541 aaataaacca tttttagttc aagtagaaaa atcttatgaa aattctacta tcagcaaatt
     3601 attagaatta gtagaaaatg ccagcagtaa aaaagcacca gctgaaaatt tcattactaa
     3661 attcgcgcgc tattacacac cagtcgttgt aggattggcc gttttattag ctgttttacc
     3721 tccacttgtt gtttctggag cggcgttttc tgaatggatc tatcgagcat tgacattttt
     3781 agtgatctca tgtccatgtg cattagtgat ctccgtaccc cttagtttct ttggcggtat
     3841 cggaggagca agtaaaatcg gtgtattagt aaaaggttca aattacttgg agttgcttgc
     3901 acaaactgag acagtggtat tcgataaaac cggcacatta acgaaaggtg acttttcgat
     3961 ccagacgatc gatacaaaaa tcgatccaaa gatattcatg caatatgttg cgagtgcaga
     4021 acagttttct acacatccga ttgctcaatc tgttttagaa ggctacgcag gtccgctttt
     4081 accaacagct aatatccaag aatttgcagg tgaagggatc ttagctgaag ttgatggcaa
     4141 acaagtatta gcaggtaatc acaaattgat ggaaagattt gaaatctctt ttccttcaag
     4201 tcaagaaatc ggtacattgc tttatctagc gatcgatcaa agttacagtg gttatcttgt
     4261 tattgcagat accttgaaag aagatgccgt agacgcattg gttcaattaa aaaaggctgg
     4321 tgtgaagaac acagtcatgt tgacgggcga ttcgaaaaaa attgctgatc atatcgggaa
     4381 gcaagtaggc gtggacaaga tatatagcga attgcttcca gaagataaag tgcagcggtt
     4441 agaggaaatc ttacaaagaa acaataaaaa gaccgcattt gtcggagatg gaatcaacga
     4501 tgcaccggtg ctagcaagag cagatgtagg aatcgcaatg ggtggattgg gaagcgatgc
     4561 tgcaattgaa gcagcagatg tagtcatcat gaatgatcaa ccatcgaaga ttgcagaagc
     4621 aatccattta gcaaagaaaa cattgaaaat cgtcaaacag aacattgtct ttgctattgg
     4681 gataaaaatt ttggtacttg ccttaggtgc ttttggtttt gcatcaatgg gagatgcagt
     4741 atttgcagat gttggggtga cagtattagc tgttttgaat gccatgcgaa gcttgcatgt
     4801 aaaaaataaa cgttgatcaa tgaagaggac gaatgaacgt cctctttttt ttatatcctt
     4861 ttcttaatga aaaatgtaaa gaaaatagat aggattataa aataactaat gtttttaatt
     4921 ttgcagaaaa ttcaaaatag cttgaaattc aataaggtaa atgaaataaa agaaaataac
     4981 ttattttttt gatggaaaaa atatgaaaaa acgatgtgta ttaaataaaa gaatacacac
     5041 ataaaataac actgtcttgt aagtttgtta aaaattgaat atgattacat ctggataaca
     5101 aatttttatc ataaaggaga gaacgaatga acaacaaaca attacgctgg tttactgttg
     5161 cgttaattgc atttaacatg gtttggggca tggggaatgt agttaataac tatgctcagc
     5221 aaggagtatc agttgtcacc tcttggctat tgattttggc catttatttt attccatacg
     5281 cattgatcgt tggacaatta gggtcagcct tcaaggatag taaaggaggc gtcagttcat
     5341 gggtcgaaaa tacgacatct aataaacgac tggcatacta tgccgcttgg acttactggg
     5401 tcgtccatat tccttatttg gcacaaaagc ctcaagcaat cctgattgct ggcggttggg
     5461 cagtccaagg aaatggaaat atcgtaaata cgatgtcagt acaaatggta gcagttattt
     5521 cacttattat atttcttgcc tttttatatt tatcaacaaa aggactttct actttgaaag
     5581 tgattggcgg actggccgga acagcgatgt tcgtcatgtc gatcttattt attcttttag
     5641 ctgtaacggc accgttcatc aatccaacga tggaatttgc cacaccacat atgaatcaag
     5701 tgaaaacata tattccaaca tttgatttca gttatttcac aaccgtttcg atgctagtat
     5761 ttgcagttgg aggagcggaa aaaatatccc cttatgtcaa ctctacaaga aatcctgcaa
     5821 aggaatttcc taaagggatg atcttcttag cagcaatggt agggatttgt gctgtattag
     5881 gctcagtcgc aatgggtatg ctgtttgcta gcgataatat tcctaaagac ttgatggcaa
     5941 atggggcata cagtgctttt caaattttag gtaattacta tggtatcggt aactcattga
     6001 tgatcattta cgcattgaca aacatgattg gacaaatctc cgcattagcc ttttcaattg
     6061 atgcaccact aaaaatcttg ttagcagatg cggatccaga atttgtacca aactggctac
     6121 gtaaacgtaa tcgtaaaggt acattggtga atggttatct tctgacaggt atactagtag
     6181 gaatcattat tttgttgccg atctttggta tcaaagatat gaatgaatta gtaaaatggt
     6241 tgacgaactt gaactcagtt gtcatgccaa tgcgttacct atgggtgttc ttggcatata
     6301 tgatgttgaa taaagcttat aagaaattca attctgaata taaatttgtt aaaaatccaa
     6361 aaatcggttt tatctttggt gcttggtgct ttgccttcac tgcgtttgca tgtatcctag
     6421 gaatggtgcc aaaagtagat tatactgctg atcctaaagc atggtggttc caattattca
     6481 cgaatatcat cacaccaatt gtcttgatct tattaggtat gatcttgcca gcaattgctc
     6541 gtcgagacaa aaacaaagaa atcactgaac cagaaacagt ggttcagcct taaaatagaa
     6601 atacttgtta
//
LOCUS       DACEUZ010000124         6434 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.124, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000124 DACEUZ010000000
VERSION     DACEUZ010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6434
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.124"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(434..976)
                     /locus_tag="IXG44_002223"
     CDS             complement(434..976)
                     /locus_tag="IXG44_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1658925.1"
                     /translation="MSIKETTQITRQLNAIYKAAHLLQEHFVDKKVSFVGEVSTVAII
                     FSTTNFMHLCGIDYRRGTHLFFQDALDRKINLQDIQIKTDGTTFQKLQVIGSLDLLLG
                     KHISIVGRGVYSSLRYDAAIRTRKKILALSLKQNGLIYIPISLLNLSSKEIGPGQKVT
                     GIFSEDLTSGELKMIMEVID"
     gene            complement(1289..1840)
                     /locus_tag="IXG44_002224"
     CDS             complement(1289..1840)
                     /locus_tag="IXG44_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ1658926.1"
                     /translation="MGYNVQPLRSQQEINDFLFCLRRNKNAERDVFLFLIGINSGLRM
                     SDIVKLKKKDVITSKNPRIVEQKTGKTRILYLSSLQDLIHGYTAPLDPEAYLFPSTKG
                     GHLEVNTVYQMFQKVAKLLGRDDIGTHTLRKTFGYHYYKKTKDVATLMEIFGHSSEKI
                     TKRYIGINEDEISETLLNFRLGF"
     gene            complement(2133..4064)
                     /locus_tag="IXG44_002225"
     CDS             complement(2133..4064)
                     /locus_tag="IXG44_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAQ1658927.1"
                     /translation="METIQEQQSQEKHNHNHNHDHDHDHGKMPVVLYFVGLALAVIAL
                     FLNEDYQLVQNVLFSIATISAGYHVILLEGLGETIENSKAQKKFIPNSHILMGLAAIG
                     ASLMGNFWEGTLLILIFSGAHFLEDYAEGRSKREITKLLEMNPTTARLILPDGSTKNV
                     DVSELKVGDQLQVLNGDQVPIDGVILSGSTSIDESSITGESIPKEKSKGDGVFGSTIN
                     GTGTFTMKVTKENKDTVFSKILQLVNQNQDNQTKAASIIQKFEPKYVTVVLIAIPLFM
                     LLAPFLLDWTWSQSVYRGLVLLVAASPCALAAATVSATLSTTSNLAKKGVLSKGSSYL
                     SQLADIKAIAFDKTGTLTKGKPEVTNYYFADSVNEENMIDIVVALEKESNHPLADAIL
                     RKFEQKNKLTIEVENQIGKGLTGDYKGRNYRIGKPTSFDDVSDEYSRLNNEWASEGKT
                     VVYVAVDEEVIGLIALMDVPSEYAKETIEYFRKQGIHTTLITGDSEMTGKAVAKQLGI
                     DEVIANVMPEDKSRIIDEQKEKYGVTAMVGDGVNDAPALVNADVGIAMGDGTDVAVEV
                     SDLVLMQNNLSKLVQSHNISSKMNRVIWQNIIFSMAVVAFLIVVSFLGLTDIAISVII
                     HEGSTLVVILNGLRLLKAV"
     gene            4363..4677
                     /locus_tag="IXG44_002226"
     CDS             4363..4677
                     /locus_tag="IXG44_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAQ1658928.1"
                     /translation="MDIKTTSPIDKGSILSISKLFKVISDPTRLSVLFLLQKKECSVG
                     NIAISLDMEQSAISHQLKTLKDARLVKSRREGKSMLYSLDDLHVFSILEQVLTHVNEL
                     EK"
     gene            4719..5627
                     /locus_tag="IXG44_002227"
     CDS             4719..5627
                     /locus_tag="IXG44_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAQ1658929.1"
                     /translation="MSKSDKDHNHSNQNQPKKNIKIAFFINLIFSISEFFFGFLFNSV
                     SIMSDAVHDLGDSISIGFAWFFQGYSEKKQDERFTFGYNRFSLLGALITGVVLISGSF
                     FMIYRSVPRLIDPQPVNYSGMFWLSLVAIALNGYAAWILSKGTSKNESMLNLHMLEDV
                     LGWIGVLIVSILMNFTEAYRLDPILSILIALYILYKTVPEFFTTMKILLNGSPENVNV
                     KSLADSIKNVPAVKDLSHFHIWSLDGEENALIITVLIDPSDIDKAREIKEEIAEVAHK
                     SSVENHITIEITTSKEELEKGYSHNT"
     gene            complement(5729..6340)
                     /locus_tag="IXG44_002228"
     CDS             complement(5729..6340)
                     /locus_tag="IXG44_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HAQ1658930.1"
                     /translation="MLQSIFSAIAVYISTSIDYLFILLIIFSQSHTKKGIRHIFWGQY
                     LGTGILVAVSLFAAYVLNFIPQDWIIGLLGLIPIFLGIRVAFVGEEEEEEEEVVEKLE
                     SRGTNRLFWTVALITIASGGDNLGIYIPYFTSLAFSEIIIALIVFAISVAVLCYISYK
                     LAKISFVSETLEKYERIIVPIMFIALGIFIMIENGTIQTLLGF"
BASE COUNT         2020 a         1243 c         1095 g         2076 t
ORIGIN      
        1 ttaagattaa tgacactaaa ttaacagact atacgtagta tctatttgct atgaccatat
       61 cttatatcca taagtcaact cagccggact aaaagggcta atcttactag aacacaagct
      121 gcacttgata tgcaaattaa ccctgaatcg gaaaggtgct taaattggat attaacggag
      181 ggataaagta aaatttatct ctagggaatg tttagaaagg atccaagttc cttaacaagt
      241 gacaattagt aatgtagtaa caacgatagg ttgtatgccg aacatttcaa tttgatacgt
      301 ataaaagacg atttcaagct catccgtgat aaataaccca catgaatgat ttgaaatcgt
      361 cttaaaatgc tttattttat aacccaaaat agaataatta cttcaaaatg acgtcgcata
      421 tcactattaa tgattaatcg atgacttcca taatcatttt aagttccccg gatgttaaat
      481 cctcgctaaa aatgccagta actttttgtc caggtccaat ttcctttgat gataggttta
      541 acaaagagat agggatatag attaatccgt tttgtttcaa agacagagcc aatatttttt
      601 tccttgttcg aatagctgca tcatagcgaa gagaagagta aacacctctg ccaacaatag
      661 aaatatgctt ccccaataat aaatcaaggc tacctattac ttgtaatttc tgaaaggtcg
      721 tcccatccgt tttgatttga atatcctgta aatttatttt cctgtctaag gcatcttgaa
      781 aaaacaagtg tgtgcctctg cgataatcaa tgccacagag gtgcataaaa ttagttgtcg
      841 aaaaaataat cgctaccgtt gagacttctc cgacaaagga tactttttta tctacaaaat
      901 gttcttgtaa gagatgagca gccttataaa tcgcgtttaa ctgtctggta atttgggttg
      961 tctcttttat agacatgcgt ttgtattctt tttttgccgc gtgcgaagaa tgctcctttt
     1021 tcccaataaa aaaagctgct caaaagagca gccttttgta caggtttagt tttggtgctt
     1081 gccacccgcc ggacccttac gttccggatt tgtatcgagt ttagttttgg tgctcgccac
     1141 ccgccggacc cttatgttcc ggatttatat caagttgact atgagcctgc tctcatttgt
     1201 atatataata taccaaaaaa aggttgattt gtccaatact gtgggtgatt ataaccgtct
     1261 tactgtgctt atctattata taatacaatc aaaaacctaa ccgaaaattt aataacgttt
     1321 cactgatttc atcttcatta attccgatgt agcgtttggt aatcttctcg ctgctgtgac
     1381 caaagatttc cattaaggtg gccacgtctt tggttttctt gtaatagtgg taaccaaagg
     1441 tttttcgtag ggtgtgggtg ccgatatcgt cccttcctaa cagcttcgcg accttttgaa
     1501 acatctggta gaccgtattg acttctaaat ggccgccctt agtactggga aacaagtaag
     1561 cctccggatc taagggtgcg gtatagccgt gaatcaagtc ctgtaagctg cttaagtata
     1621 aaatgcgtgt tttccctgtc ttttgctcca cgatgcgcgg gttttttgag gtaatcacgt
     1681 cttttttctt gagtttaacg atgtcggaca tgcgcagacc gctgttaatt ccaattagaa
     1741 acaagaaaac gtcacgttcc gcatttttat tccgtcgcag gcaaaataaa aaatcattaa
     1801 tctcctgttg cgaccgtagt ggctgaacat tatagcccat gacaatccct cctttttagt
     1861 ctgttcgaat acatgattat agttattaca acacgaaatc gtgtgtttag ggggagtaaa
     1921 aaaaaagaac cctataagga taggattctt gatacatgat tttatccaat ttcatgtatc
     1981 ttatattcaa cccgcgccac aagagtatgg ttgatatatc attttgactt tgaacatcta
     2041 gataaatata aacaagtaat agagataaaa aatgtagtgt aatccttaat tatctctatt
     2101 actcgattaa tttgaagttg tgcaatcatt ctttagactg cctttagcaa tcgaagtcca
     2161 ttcaaaataa caaccaaagt acttccttca tgaataatga cactgattgc aatatccgtt
     2221 aagcctaaga aactaacgac aattaaaaaa gcaacaacgg ccattgaaaa aataatattc
     2281 tgccaaataa cacggttcat tttcgaagaa atattatgag attgtaccaa tttagataaa
     2341 ttgttttgca ttaaaactaa atcagatacc tctactgcca catcagtccc atctcccata
     2401 gcgattccca catctgcatt aacaagagca ggagcatcat ttacaccgtc tccaaccatg
     2461 gcggtcacgc cgtatttttc tttttgttcg tctataattc tggatttgtc ttccggcatg
     2521 acattcgcaa tcacttcatc tattcctaat tgtttagcaa ccgcttttcc cgtcatttct
     2581 gagtcaccag taattaatgt ggtgtgtata ccttgtttcc taaaatattc aatggtttct
     2641 ttagcatatt cactcggaac gtccataagg gcaataaggc ctataacctc ttcatctact
     2701 gctacgtata cgactgtctt accttctgac gcccattcat tatttaaacg actatattca
     2761 tctgaaacat catcaaacga agtcggtttt cctatacgat aatttctccc tttgtaatct
     2821 cccgtcaatc ctttaccaat ttggttttct acttcaatag ttagtttgtt tttttgctca
     2881 aactttctta gaatagcatc tgctaagggg tgattggatt ctttttcaag agctactacg
     2941 atatcaatca tgttttcttc gttcacggaa tcagcaaaat aataattggt tacttcaggt
     3001 tttcctttgg tcaaggtgcc cgtcttatca aatgcaattg ctttaatatc ggctaattga
     3061 gacaggtaag aactaccttt tgaaaggact ccttttttgg ctaaattaga agtcgtcgat
     3121 agcgttgccg agacagtagc tgctgctaaa gcacacggtg aagctgcaac taaaaggacc
     3181 aatcctctat agacactttg tgaccatgtc caatcaagta gaaaaggagc caataacatg
     3241 aatagaggaa tggcaattaa aacaactgtg acatatttcg gttcaaattt ttggataata
     3301 ctcgcagctt ttgtttggtt atcttggttc tgattcacta attgtaaaat ttttgaaaat
     3361 actgtatctt tgttttcttt ggtgactttc atcgtgaagg tacctgttcc attgattgta
     3421 ctcccgaaga cgccatctcc tttggatttc tctttcggga tactttctcc agtaatagac
     3481 gactcatcaa tggatgtaga tccagacaaa atgacgccat caataggtac ttgatcgcca
     3541 ttcaacactt gaagttgatc tcccactttt aattcactga catccacatt ttttgtacta
     3601 ccatcaggca ggattaaccg ggcagtcgtt ggattcattt cgagtaattt cgttatttct
     3661 cgtttgcttc ttccttctgc ataatcttcc aaaaaatgtg cgccagaaaa aataagaatc
     3721 aataatgttc cttcccaaaa attccccatc aaagaagctc caattgccgc taatcccatt
     3781 aaaatatgag aattaggtat gaactttttt tgtgcttttg aattctcaat tgtttctcca
     3841 aggccttcaa gaagaataac gtgatacccg gcactgattg tagcgattga gaacaaaaca
     3901 ttttgtacca actgataatc ttcattcaaa aatagagcaa tcactgctaa tgctaaacca
     3961 acaaagtaca aaacgactgg cattttccca tgatcatggt cgtgatcgtg gttgtgatta
     4021 tgattatgct tttcttgaga ctgttgttct tggatagttt ccacttctat tgcctcccta
     4081 tataatttat aattcctttg ttttccatca tttacttctt gaatcacata aatttctgct
     4141 atttctgact cctttcgttt attccaaaag taaatcgtaa tgaatccacc ttacatatga
     4201 ctaattaatc atatgtttgt atactcattt tatatcgcta tatacatttt gtcaagataa
     4261 agagaacgcc acttctcttt tataaacaaa agattagcat tgtttcgctc atatggtaat
     4321 atagatatga acggaaggtc atatgaaagg aataaaaaca ctatggatat aaaaacaact
     4381 tctccaatcg ataaaggatc tattctgtca ataagcaaac tatttaaggt gattagtgat
     4441 cctacacgat tgtcggttct ctttctttta cagaaaaaag agtgtagtgt tggaaatatc
     4501 gctatttctc tggacatgga acagtccgct atttctcatc aattaaaaac gttgaaagac
     4561 gcacgcttag tgaaatcaag aagagaagga aaaagtatgc tttacagttt agatgacctt
     4621 catgttttta gtattctcga acaagtctta actcatgtta atgaattaga aaaataaaac
     4681 caattagtat gtagcataca aacttaggag gtttttaaat gtcaaaatca gataaagacc
     4741 ataatcactc gaatcagaat caaccgaaaa agaatattaa aattgctttc ttcataaatc
     4801 ttattttctc tataagcgaa tttttctttg gctttctttt caatagtgtg tccattatgt
     4861 ccgacgcagt tcatgattta ggggactcta tttcaattgg attcgcttgg ttttttcaag
     4921 gttattcaga gaaaaaacaa gatgaacggt tcacttttgg ctataaccgc ttttctttac
     4981 tgggcgcgct gataactggc gttgtgctaa ttagtggctc gttttttatg atctatcgta
     5041 gtgtgccacg tttgattgac ccacaacccg taaattatag tggcatgttc tggctttctc
     5101 tcgtagctat tgcattgaat ggatatgctg cgtggatttt aagcaaaggc acatccaaaa
     5161 acgagagcat gttgaacctc catatgctgg aagacgtatt agggtggatt ggagtcttga
     5221 ttgtgagtat tctaatgaac tttaccgagg catatcgtct ggatcctatc ttatcgattt
     5281 tgattgcact ttatatcctc tacaaaaccg ttcctgaatt tttcaccaca atgaaaattt
     5341 tattgaatgg ttcgcctgaa aatgtaaacg taaagagctt ggcggactcc attaaaaatg
     5401 ttcctgctgt aaaagacctc tcacattttc acatttggtc actggacggg gaagaaaacg
     5461 cccttatcat cacggttctt atagaccctt cagacataga caaggctagg gaaataaaag
     5521 aagaaattgc agaagtcgca cacaaatcaa gcgtagagaa tcatatcaca atagaaataa
     5581 ctacaagcaa agaagagctt gaaaagggat actcccacaa cacgtaagat agttctacgc
     5641 taaaaaatac ttcattattt gaaaagcaaa aaaggcagcg attttgatta gtcgctgcct
     5701 ctctttttat ctttcgtgat tatgattttt aaaaacctag tagcgtttgt atcgtcccgt
     5761 tctctatcat aataaaaatt cctaacgcaa taaacattat agggacaatg attcgttcgt
     5821 atttttctaa cgtttcggaa acaaaagaaa tcttggctaa tttgtaactg atatagcaga
     5881 gaaccgcaac agagatagcg aatacgatta aagcaataat gatttctgaa aaagctaacg
     5941 aggtaaagta tggaatataa atacctaaat tatcgcctcc agaagcaatc gttattaagg
     6001 caactgtcca gaataagcga ttagttcctc tagattcgag tttttcaacg acttcctctt
     6061 cttcttcctc ctcttcgcca acaaaagcga ctctgattcc taagaaaatc ggaattagac
     6121 cgagaagacc aatgatccaa tcttgcggaa taaaatttag cacataagca gcaaacaaac
     6181 ttacggctac caaaatacct gttccaagat attgacccca aaaaatgtga cgaatacctt
     6241 tttttgtgtg gctttgagaa aagataatca gcaagataaa caaataatca atactggtag
     6301 aaatataaac agcgatagct gaaaaaatac tttgtaacaa aataacttcc tccaaactaa
     6361 gatttcatag atagattttc tgataacttt aaaaaaacac gaatagcgga aaggatgtct
     6421 tcattcgcta aaga
//
LOCUS       DACEUZ010000125         6361 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.125, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000125 DACEUZ010000000
VERSION     DACEUZ010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6361
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.125"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(186..731)
                     /locus_tag="IXG44_002229"
     CDS             complement(186..731)
                     /locus_tag="IXG44_002229"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAQ1658931.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(735..863)
                     /locus_tag="IXG44_002230"
     CDS             complement(735..863)
                     /locus_tag="IXG44_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAQ1658932.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGTQLTDKEIR"
     gene            complement(856..1947)
                     /gene="phnW"
                     /locus_tag="IXG44_002231"
     CDS             complement(856..1947)
                     /gene="phnW"
                     /locus_tag="IXG44_002231"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HAQ1658933.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            complement(2164..2643)
                     /locus_tag="IXG44_002232"
     CDS             complement(2164..2643)
                     /locus_tag="IXG44_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HAQ1658934.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            3002..3784
                     /gene="rpsB"
                     /locus_tag="IXG44_002233"
     CDS             3002..3784
                     /gene="rpsB"
                     /locus_tag="IXG44_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HAQ1658935.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3883..4764
                     /locus_tag="IXG44_002234"
     CDS             3883..4764
                     /locus_tag="IXG44_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HAQ1658936.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            4900..5622
                     /locus_tag="IXG44_002235"
     CDS             4900..5622
                     /locus_tag="IXG44_002235"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010761216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HAQ1658937.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            5625..6182
                     /gene="frr"
                     /locus_tag="IXG44_002236"
     CDS             5625..6182
                     /gene="frr"
                     /locus_tag="IXG44_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HAQ1658938.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
BASE COUNT         2126 a         1172 c         1279 g         1784 t
ORIGIN      
        1 gaggagctat ttttgcttgg ctgtttttat tacttgatac aggacagctt tttcacaacc
       61 tcttattttg tataaaagtc attctggatt cttgtcagcg tacaaaatgt attctgttaa
      121 ctcttttaaa ttgtttacac aaatatcagc gccggcttgt tggaatctaa gagcaacaga
      181 ctgttttaaa cgatcttttt ccccatctct aatttttggt actcatcata agaaatcccc
      241 attagcgaac taccttcaat tacacctact gtcaacactc cggcattttt cccttcctgg
      301 atatctgaga ccgtatctcc gactttgatt acttcatcaa ccgacttgtt tttgaagtac
      361 tgcatgtttt tatggatcat ataaggaaac ggtcgaccat acccttcgac gtcttcaaaa
      421 gttgccatga agtctggtga atacctttct ttttttgcgg tttctgctac gattgtcatc
      481 ataggagcat taaatccatt cgtggaacca atattgatcc cagcgtttct caaccgttcg
      541 atcgtttcca atgtgtcggg tttcaaggta gaatgcttga ccaaatcttt aaacagataa
      601 tgttcaaatt gttgatacaa ttcctccacc tctttttcct gaggtagttg tccataaata
      661 gctatccatt gttttttgac agaagggagg gaaagaagct gttggatatg atcccatttt
      721 aattttccca tcggttagcg gatttctttg tcagtcagtt gggttccttt ttctagaaaa
      781 gcatttctaa aagcgtggac aggagccata cagccaaagt ccacagttgt tcctgcccaa
      841 tcaaaaatta cagttttaat cattaacgtt ctccttatag tctttgataa ttgctgtcag
      901 ttccaaaata tcttccggat agatctcgcc aatatttccg atacgaaaac attcatgatc
      961 gctgattttc ccgggataga tcacaaatcc attttgcttt aaatactgat aaaaatgatt
     1021 aaaatcaaat cgccagtcct tagggtaaag aaaagaggtg atgaatggcc cctgatattc
     1081 ttgaatgaaa ggctggaacc ccatctctgt cattttttgt cgtagcacat gattattggc
     1141 gagataacgt gaatatcgag tttcgattcc tccttctgcc tctagttcct tcagcgcttg
     1201 ttgaaaagcc aaaaccgtat gggtagggga agtgaaacgc catttgccat tctcgttttc
     1261 catcacgcgg tactggtcat atagatcaag tgacaaactt cggctaacat tttttcgttt
     1321 ttccagttcc gctttattag caagtacaaa agaaaagcct ggaacacctt gaatacattt
     1381 gtttgagctg gaaatcaaaa aatcaatccc tagttcggct acacaaatcg gtatcccacc
     1441 aaagcttgac atcgcgtcca ctataaacaa aagatctctt tcttgtgtga cagcagcaat
     1501 ctcttccaaa ttattcaaaa taccggaagt agtctcgcta tggaccattg ctagtgcgga
     1561 gatagacgga ttctcttcaa tcagctgtgt aatcacatga gcttggagga tttgatgttc
     1621 cgatacctca taaacggtat agttcagttt cagacattcg cacatgttga tcattcgttg
     1681 tctatatgcg ccgtttgaac aaatcagtac agtatcttct ggcctgatca cagaacttaa
     1741 taccgattct accccgaagg agctgctacc ttgcattaag accgttgtgt atattccttc
     1801 cgatacttga gcaagatcca ataatttttt tcttatccat tgtgtaattt ctttgtattc
     1861 ttgatcccaa gtacaatgat cgatcagcat agcatttttc actgtttcag aagtcgtcaa
     1921 tggtccagaa gttaataatt tatacatatg atttcctttc atttcttttt tgaaatacac
     1981 aaagagcaag aagctgctct tgttaacatc caatataaat gatgattatt gtgattcaat
     2041 caaggtgttg aaaaatttga ataaaaaatg atgtattttt gtaaagagtg taaaggttta
     2101 ttcaataagg aaaatattga aaataaaaaa acacgcattc catattagga atacgtgctc
     2161 tgattaaaaa agtgatactt cagttggatg gtgaagaaat tcttctactc gttttgcgtc
     2221 ctcctcattt tccgagatga ccatgatcgt atcgaatcca gcaattgtac tgacaataga
     2281 gggaggtttg atctcatcta aaacagatgc aaataaatga gcattgtctg gcaaggtatt
     2341 gacgatcgtc aaaaagtgga ctcgttcgac tttcaataca atgtcttcaa tcatttgaac
     2401 cagccgatcc tcttcctcag tcctagtctc aggagttcct ttttcgcgaa acaatacgaa
     2461 gcgggaaacg ccattttcat caggtgcctt cacgattttt agatcacgga tatctcgaga
     2521 aatcgttgcc tgcgtggcgg taacgccata atgcccaagt ttgtttaata gttcttcttg
     2581 cgtacggatc gggaattctt caatgacttg tttaatcagc aaatgtcttt gagctttgcg
     2641 cattttcttt ctcctttacg aaagatttca catgtatcat aacaaaaaaa agaaggattg
     2701 cccaataata ttcgtctttt atacctaaaa ttattttatg gaaaaaggat aataaagaaa
     2761 caaagagaaa tctactgttt ttcttgaatt ctttttatct ttcaggtaaa ctattgaatg
     2821 ttgagcagaa aagtatattt gctcttgcga tacggatctt ttgtctgtat aatagctgag
     2881 ggtgctttat gcatcagcaa atccacattc ttattggatt gtcggtaggt gcttgaaaaa
     2941 gtttgccggc aaggtagaag ataagaagag gaaaaataaa accaaatcgg aggaaacaaa
     3001 catggcagta atttctatga aacaattact agaagccggc gtacactttg gtcaccaaac
     3061 tcgtcgctgg aatccaaaaa tgaagaaata tatcttcaca gaaagaaacg gaatctacat
     3121 cattgacttg caaaaaacag ttaaattagt agatgcagct tatgattaca tgaagagcgt
     3181 tgctgaagaa ggcggcgtag cattattcgt aggtacaaaa aaacaagcac aagaagcaat
     3241 caaagaagaa gcaactcgtg caggacaata cttcgttaac catcgttggt taggcggaac
     3301 attgacaaac tgggatacta tccaaaaacg tatcagccgt ttgaaacaaa tcaatgcaat
     3361 ggaagaagat ggaacttttg aagttctacc taaaaaagaa gttgctggtt tgaacaaaga
     3421 acgtgaacgt cttgaaaaat tcttaggcgg tatcgctgat atgccaagaa tcccagatgt
     3481 aatgtacatc gttgacccac gtaaagaacg tattgctgtt caagaagcac acaaattaaa
     3541 tatcccaatc gtagctatgg ttgatacaaa ctgcgatcca gatgaaatcg acgtagtaat
     3601 cccttcaaac gacgatgcaa ttcgtgccgt taaattgatt acttcaaaaa tggctgatgc
     3661 tttcatcgaa ggaaaccaag gtgaagatca agtagttgaa gaagatttca cagaagaaaa
     3721 cacagcaaca tcaatcgaag aaatcgtaga tgttgttgaa ggcgacaatt cttcagcaga
     3781 ataattttga atgatggagc tgtttcaaag gctgggcgac aagcctcact ctcctttgaa
     3841 gcagcttttt ttaaaaagaa aaacacacag gaggagttaa acatggcaga cgttacagct
     3901 aaaatggtaa aagaactacg cgaaatgact ggcgtaggaa tgatggatgc gaaaaaagca
     3961 ttagttgaag tagaaggcga catggaaaaa gcagttgatc ttttacgtga aaaaggaatg
     4021 gcaaaggctg cgaagaaaaa tgaccgcatt gcagctgaag gattggcttc agttgctgtg
     4081 aaaggtaaca ctgcagcaat cgttgaagta aactcagaaa cagactttgt ttctaaaaac
     4141 gaaatgttcc aagatttagt aaaagaaatt gcagaacttg ttgctgaaaa caaaccagca
     4201 gacatggaag ctgcaatgaa aatcaaaaca gataaaggta caattgaatc tgacttgatc
     4261 gaagcaactc aagttatcgg agaaaaaatc agcttccgtc gctttgaagt tgtagaaaaa
     4321 gacgacaatg ctgcatttgg tggatacttg catatgggcg gacgtattgc cgtattgact
     4381 gttctagatg gaacaactga cgaaacagtt gcaagagacg tagctatgca cgtagcagct
     4441 atcaatcctc gttatgtaaa cgaatcacaa attccagaag ctgaattaga acatgaaaaa
     4501 actgttctta cagaacaagc attgaacgaa ggcaaaccag cgaatatcgt tgaaaaaatg
     4561 gtagaaggac gcttgaagaa attcaaagca gaaatcgctt tggtggacca accattcgtt
     4621 aaagatcctg atatgactgt tgaaaaatac gttgcatcaa aaggcgcaac tgttaaaaca
     4681 ttcgtacgtt tcgaagttgg cgaaggtatc gaaaaacgcg aagataactt tgtagaagaa
     4741 gttatgagcc aagtgaaaaa ataattctgt tgaataacag acgggaacgc acggtttgtg
     4801 cgctcccttt ttttaagcaa aacaagaaaa accagtctta gagtgtcgaa atcatctaag
     4861 tatggtaaaa tggcaataga aaatattgga ggaatgaaca tggtcacacc gaaatatcag
     4921 cgtgtagtct taaaattgag tggagaagct ttagctgggg acgcaggatt tggaatcaaa
     4981 ccaccagtaa tcaaagaaat catccaagaa atcaaagaag ttcacgagct gggcgttgaa
     5041 atggcaatcg ttgtgggcgg cggaaatatc tggcgcggac aaatcggagc acaaatgggc
     5101 atggaacgtg ctcaagctga ttacatgggt atgttagcta cagttatgaa tgccttagct
     5161 ttacaggata cgttggaaaa cgtgggtgta ccgacacgcg tgcaaacttc gattgaaatg
     5221 cgtcaaatcg cagagccata tattcgccgt aaagcagaac gtcatttaga aaaaggacgg
     5281 atcgttatct ttgcaggcgg taccggcaac ccgtacttct cgacagacac aacagcagca
     5341 ttacgcgcgg cagaaatcgg cgcagatgtt atcctgatgg ctaaaaacaa tgtagacggt
     5401 gtttattctg ctgatccgaa agtagatgca aatgcagtaa aatttgaaga actgactcat
     5461 ctggaagtta ttgccaaagg attacaagtc atggattcaa ctgcaagttc actaagcatg
     5521 gataatgata ttccactgct tgtcttcaat ttaaatgaac acggaaatat tcgccgtgca
     5581 atcttaggcg aaaatatcgg aacaactgta agggggaaat aaaaatggca gatgcaatta
     5641 tgacagaagc aaaagaaaaa atgaaaaaag cagcacaaaa tcttcaacgt gaacttgggc
     5701 aaatccgcgc aggccgtgcg aacgctagcc ttttggaccg tattactgtg aactactacg
     5761 gtgcgccaac accattgaac caaatggcat caatccaaat tccagaagca cgtgttttaa
     5821 tgatcacacc ttttgacaag agcattcttc aagatgttga aaaagctatt atggcaagtg
     5881 atatcggaat cagtccaacc aatgatggaa atgtcattcg cttagttatc ccgcaactga
     5941 cagaagaacg cagaaaagaa ttagctaaag atgtgaaaaa agaagcagaa aatgcaaaaa
     6001 tcgcgattcg taacatccgt cgtgatgcga tcgatgaata taaaaaacaa caaaaaaatg
     6061 gtgatattac cgaagatgat ttgcgcggat tagaaaaaga cgtacaaaca ttgacagata
     6121 acagcatcaa agaagtagat aagatcgctg ctgataaaga aaaagaatta ttggaagttt
     6181 aataaaatca taaaaacaga ggggcggatc attccgcttc ctctgttttt atttatattt
     6241 cttttattta aaaattaaaa aaagaaaaaa ttattcagtt tttaaataaa aaaacaatga
     6301 tttcatagga aaaacagtta gatattgcta taatacttaa gatagtggga atcttttaag
     6361 t
//
LOCUS       DACEUZ010000126         6077 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.126, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000126 DACEUZ010000000
VERSION     DACEUZ010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6077
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.126"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(12..2183)
                     /gene="copB"
                     /locus_tag="IXG44_002237"
     CDS             complement(12..2183)
                     /gene="copB"
                     /locus_tag="IXG44_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HAQ1658939.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(2203..4389)
                     /locus_tag="IXG44_002238"
     CDS             complement(2203..4389)
                     /locus_tag="IXG44_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HAQ1658940.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGWIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(4389..4598)
                     /gene="copZ"
                     /locus_tag="IXG44_002239"
     CDS             complement(4389..4598)
                     /gene="copZ"
                     /locus_tag="IXG44_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HAQ1658941.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(4611..5051)
                     /locus_tag="IXG44_002240"
     CDS             complement(4611..5051)
                     /locus_tag="IXG44_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HAQ1658942.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(5125..5664)
                     /locus_tag="IXG44_002241"
     CDS             complement(5125..5664)
                     /locus_tag="IXG44_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation protein"
                     /protein_id="HAQ1658943.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQSFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
BASE COUNT         1576 a         1368 c         1068 g         2065 t
ORIGIN      
        1 ttttttcatt attatttgat tttcaatgtc aaagcattta gagctacaac taccgtactt
       61 aacgacatca agacagcacc aactgctgga cttaatatga taccaattgg cgcaagaatt
      121 ccggctgcta gcggaattgc aagaatattg taacctgccc cccaccagag gttctggatc
      181 attttctttc gcgtttgctt ggctagatca agaaaatgca aaatatcttt tggatcgctg
      241 tttgtcaata cgacatccgc agaatcgatt gcgatatctg tccctgcacc gattgccatc
      301 ccgattgaag cacgggctaa acttggcgca tcattgatcc catcacccac catgattact
      361 tttttccctt ggtcaaggta tttctggaca acagcttctt tatcatctgg taataaaccg
      421 ccataatatt cttcgatccc taaataatct gctactgctt gggcagcgtt tttattgtca
      481 ccagtaagca tgacaggagt gatttttctt tctttcaatg ttcggataaa ttcttttgct
      541 tctgttttta ctacgtcacc taaagcaacg attgcagcta gatgattttc taaaatcagg
      601 aagctgatgg tatttccttg ttcttggtaa ggtttcaata atgcttgttc tacgtttaat
      661 ccgagacgct caacttcttt ttcattaact attttcactt gttgtttttt gactgttgcc
      721 tctagcccga ctcctgaaag attttttaga ttttcagctt gataaggctt gatttcatgt
      781 tctttcaaat aattcataat gccaacagcc aatggatgat tggcattcgc ttccaaggct
      841 cctagatatt gaaggatttc atttttattg aacgcatcat caagtacttc gacacccgtt
      901 acagtaaatt ttccttctgt tagtgttcct gttttgtcca gcatgatcac atctagatca
      961 ttggcttgct ccagtgcatt gcggtttttc aaaagtaatc cgtttttagc tgctattgat
     1021 gtggagcgag caacaactaa tgggatagct aaacctagag catgaggaca ggcgatgata
     1081 aagactgtta ccatacgttc taatgcatta ggaagatctg tcaagaacaa ccaggcgatg
     1141 aatgccagga tacccacaat taatgcgaca taaaacagcc attttgccac tttgtcagat
     1201 aatgactcta gcttggattt ttctccttgt gcttttcgca ccatttccat aaccttcgct
     1261 agataaccat tttcaccagt tccagttact tcgatttcga tcgttccatc cccattgata
     1321 gaaccgccaa tcactttgtc attggcttgt tttttgattc ctcttgattc gccagtcaca
     1381 gctgattcgt caacaattgt ttcacctttc aagattacac catctgttgg cattttatca
     1441 ccagaccgga cgatcaaacg atcaccttcg tttacttctt tcaatgaaac tgtctcttct
     1501 tttccatctt tcgtcaagcg tttcactgat tctggcagta attctgctag tttttgtaaa
     1561 gcattgctgg cattcgacac agcattcatt tcaatccagt gacctagtag cataataacg
     1621 atcaaagtag ccaattccca gaagaaatcc atgacgtgtg tatgtggatt gagcagattt
     1681 gcgataaacg aatagacact ataaatatat gcaactgtaa tccccatcgc gatcagtgtc
     1741 atcatagcag gacttttttg tttcaattcc atcttagctc cacttaaaaa tggctgaccg
     1801 ccatagataa atagtatagt tgctaagatc aatacgagcc aatcagaacc aggaaacgtt
     1861 acttgaaatg gaaattccat tcccatcatt ggtgaaaaaa gaatgattgg gatagctagg
     1921 ataagcgata gccaaaattt ttgtttgaaa ttccccatgt gcatggaatg atccatgccg
     1981 ccgtgttcgt gatccatgtg catcgaatgg tccatttgtt tgctgtttgc tatatctcca
     2041 tggttatgtc ccatgtgcat ggaatgatcc atctgactat ggtcatgttt catctccatc
     2101 tgtttttcat gagattctgt actttctttg tttactactt cttttttatg ctcatgtttt
     2161 ttctccatat cgttcccatt catttttact ccatcccttc tcttactttt tcatgtgtcg
     2221 atttaagctc agggagttca acaatacact gacggaacta aacgccatcg ctcctcctgc
     2281 aatgattgga ttcaaaaagc ctagtgcggc aaaaggaata ccaatcgtat tatagacaaa
     2341 agcccagaaa agattttgtt tgatttttct caatgtcaac ctagacaact cgattgtttg
     2401 tgcaatggat gccaaactgc tattcatcag cgtcacatcg gctgtttcca tcgcaatgtc
     2461 cgttccactt cccattgcca taccgacatc agctaatgct aaggccggtg catcattgat
     2521 tccatcgcct gccataccga cttttttgcc atctttctga agtttttcta cgtaggctgc
     2581 tttatcctct ggaagtactt cagcaaagat atgctttgga tcaataccaa cttgtttacc
     2641 aatcgtttcg gctgccagtt tgttgtctcc tgtcagcata aaaacatcta atcctttatt
     2701 ctggagctgc ttgattgctt gtttggcttc tagtttgatt tgatcagcca cggcaatcca
     2761 cccgatcact tgcttttcat cagctaaaaa catgactgtt tttccctgct gttctaattc
     2821 taacgcgtgt tcttggtatt catcaaatga taggttcaat tcaattaatc gtttacgtgt
     2881 acccgcaaaa tagcgtactc cattaatcgt accgctgatc ccagcacctg ggtgggctgt
     2941 aaaatcaatc atatcgtatg ttgctgcttc ttgcagtttt ccgtattcca cgattgcttt
     3001 acctagtgga tgttcagaag cttgttctaa agtataaaaa atagatagga cttcttttga
     3061 accgactaga tctgtaactt tgggctttcc ttctgtaatc gttccagttt tatccaaaac
     3121 aatgctgtct aagtgagcag ctgcttctaa tgcttctccg cctttaatta aaattccatt
     3181 tcgtgcacca acacccgttc ctaccataat agctgttggg gtcgctaaac ctaatgcaca
     3241 tggacacgca atgactaata cagagacact atggatcaat gcgagttgcc aatcaccagt
     3301 gatcaaacct gtagctatga gcgtgataaa cgcgatgact aatacgatcg gaacaaaaat
     3361 ccctgagatc cgatctgcga ttttttggat tggggctttg ctcccttgag catcttccac
     3421 catttgaatg atttgcgcta atactgtctg cttgccgact tgtgtgactt ccatgtgtag
     3481 cagaccattc gtattgattg tcccgccaaa aagcgtgtca tccggattct tttctacagg
     3541 aagattttca ccagtaagca tactttcatc tatcgcactg cttcctgaaa taattcgacc
     3601 atctgctgga atctgctcac caggacggat gactagttga tcaccaacga ctacttcttc
     3661 aatcgctaac gtttcttctt tcccgtttct tatcacttgg gcggtttttg tttgaagaga
     3721 catcaattgt ttgattgcat tacctgtttt cgtttttgct gtatgttcca aatatttgcc
     3781 gagcaaaatc agcgtgatga tcatactgct gctttcaaaa taaagatctt gggggtgtcc
     3841 acggaaaaat ccgttgtaaa tgctcaatgc aaaggcagcc gatgttccaa ttgctaccaa
     3901 tacatccata tttggtgctt tcgtcttgat tgcatggtac gcacctttat aaaaacgagc
     3961 accgacataa aattggacag gagcagacag cactaactgg acgattggaa aatgaaaaaa
     4021 gtgaacaatc gccgcatggc ttcctaacat catagcaatc attgacaaca tcaacggcaa
     4081 agtcaaaacc gtactcaata taagatcgaa tagcattttt ttcaaatacg cttgcttttc
     4141 ttctgcaatt ttttgtttgt gggcttcatc gtacaaaatt gcaccataac cgatgttttc
     4201 tacgctttgg attaattttt cagtggtcgt cccttcatat ttgacacttg ctttttcagt
     4261 ggccaagttg actgtggcat tgaccacacc aggttgtccg ttcagctctt tttcgatcct
     4321 agcagaacaa tttgcacaag tcatacctgt aatgacgaac gtatccattt tggtttctgc
     4381 tgttgccatc aaatcacctc tgctttataa cccaatccat tgattgcttg acaaatcttt
     4441 tcggattgaa cttttgtttc atcaaacttg accgtaccgt ttgctttttt caaatttact
     4501 ttcacttttt gcacgccgtc aagtgcgctt actgtttctt ctactcttgc tgcacaatga
     4561 ttgcagctca taccttcgat tgaaaattgt tgtttcattt tttcttcctc ctattgatgt
     4621 tgtttacatt cacattgacc tgggatacag ttacattcga tcgtatctac tggttgcttt
     4681 tgttccagtt gtttcaaaat ctcattgata tcttcttgag tcaaagtggc ttcttctact
     4741 agatccgcaa tcgtctctcc aatccttttt gcacagatat gggaaaaaag attttccgtc
     4801 gcactgcgaa ctgtttccat ctccgagaca gctggatgat aaatgaattt ttttccttct
     4861 tgttctgtcc agatcacttc tttttttacc aaacgtccca gcaaagtttt gatcgttgct
     4921 ggtttccaat ccatcgaatc agctaagatc tgcgtgattt cttgggctgt cgtctgtcca
     4981 agtgtccaaa taacacgcat gatctcccac tctgaatcac tgatctttac tggcgttttg
     5041 actttttcca tttatttcac acctttcgtt tacaaatgta atcattagct tatgaataca
     5101 gattacgctt gtaaacaaaa actgtcaatt atttttgctc tctattttca atttagttaa
     5161 aaattcttgt ttctctcgtc ttgttttaag gatgatcagt ttatttcttg cattagctac
     5221 ttggatcagc tgctgtattt tcggttcatt gttttcttca aaatgccaga taaaagataa
     5281 aaattctaag tattccctgt ttagtttctc tgaaaatgat tcaggcatat ctggccgaga
     5341 ttttttgttt atctttcttt ttagagaacg ccaaactact cggtagatgg cgagataacg
     5401 cggcacttta agccaaatga tcttatctgc ttctttaatc ctttcctcta atgtagaagt
     5461 ataatttcct tcaacaatcc atgacggatt agataccata aacgattgct gttcttgcaa
     5521 aaaccacttt tttgcctgaa tactgtagtc tgttgtatgc cataaaaggt ctagcggcag
     5581 tagcggaaag tcggtgattt ctttgatctt ccttgcgagg gtcgattttc ccgacccact
     5641 ttgtccaatc acgatgatct tcattcttct ccctcacttc tctttggaat ttggttcgat
     5701 cataccactt ataacgccgc aaaaaaacaa gggactgtga caaaagtcgt gtcaaagtcc
     5761 cttgtttcga ataaatggtg ttcaatcagc tgaccttcag cattaactca aaaaacggca
     5821 aaatatgagg agctattttt tcgtttgttc ctctaatact caggtcaaat cgctgatttt
     5881 acaacctctt ccttaaacgg tgttctcctg ccaacacctc ggtcacaagg cacaatagtc
     5941 aacaaacatg agaagttgtt ttttgaaatt gctccttgtg actcggagtt aaacggcagg
     6001 ctcactacct ctttcttaaa cggtgttcaa aagcttgcat ctgcggtaat aagcccagca
     6061 aaacgaaaaa tatgagg
//
LOCUS       DACEUZ010000127         6012 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.127, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000127 DACEUZ010000000
VERSION     DACEUZ010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6012
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.127"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            <1..157
                     /locus_tag="IXG44_002242"
     CDS             <1..157
                     /locus_tag="IXG44_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ1658944.1"
                     /translation="RKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQM
                     LSQPMEN"
     gene            692..1264
                     /locus_tag="IXG44_002243"
     CDS             692..1264
                     /locus_tag="IXG44_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HAQ1658945.1"
                     /translation="MTENKATQERTPQERLQEEQEHKEHVHHTKINAAAIADHLLGNM
                     HTLHVKLHQYHWYVKGANFFTLHEKFEELYNENEKWFDKLAERLITSGHKPASTTVEF
                     EKYSMLSEDAVNKYAKAEDMVENIIEDFRSTRDLTIRAIRLAQDEGDDALEDTLIAFK
                     NDLDKNIWMLQAFIGKEALEDDDAFDEDED"
     gene            1539..2156
                     /locus_tag="IXG44_002244"
     CDS             1539..2156
                     /locus_tag="IXG44_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006845390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAQ1658946.1"
                     /translation="MDRIAGKAHTKHYQKGEFLFRSQEKDDALYIVNRGKVRIYRLSN
                     SGKEQLVRILNPGDFTGEWTLFNPDAVHEEYAEATRDTSVCMIQQHDVQEFLKEYHAI
                     SLKLLGEMSQRLESSGRQTAQVAVESVITRLVLFLAENVGPEMGNSPTITLPMAKKDI
                     ASYLGTTPETISRKFGELEDEGLIQQLSGKRIKIQDLDDLLLYSE"
     gene            2308..3162
                     /locus_tag="IXG44_002245"
     CDS             2308..3162
                     /locus_tag="IXG44_002245"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003125438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HAQ1658947.1"
                     /translation="MGFVLGVLNPHVVEIGSVVIHWYGVIIAAGILAAPQLSTKEAKK
                     KGLEEDTIIDMALWAIPIGLLGARLYYVLFELGYYLQNPGQILTIWNGGIAIYGGLIA
                     GGGTLYWYAKKKGTSLALVLDILAPNVLLAQSIGRWVNFMNQEAHGGPVTRQFLENLY
                     LPEFIIEQMNMNGTYYHPTFLYESLWSLLGFVLIVTIRNRKQLLRQGEVALSYVLWYS
                     VGRFFIEGMRTDSLWIGEWIRVSQALSLALFIGAIIVWFIRRQDYPPISYYSDGNKGQ
                     KKTIKMVN"
     gene            3309..3542
                     /locus_tag="IXG44_002246"
     CDS             3309..3542
                     /locus_tag="IXG44_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003106687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HAQ1658948.1"
                     /translation="MEKAILQLETLSCPSCMQKIEGAVKSVNGVDKDSIKVLFNSSKV
                     KANFDSAVTSIEEIQKAIENVGYPVLKAKVKAA"
     gene            3740..5644
                     /locus_tag="IXG44_002247"
     CDS             3740..5644
                     /locus_tag="IXG44_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAQ1658949.1"
                     /translation="MQQYILSKKNVITVISGLLIVLGFFSHFVLENVGLSEWSLIIAS
                     VFGITPIAIQAFQAMKVKVISIDVLVSIAAIGALFIQNYEESAIVTFLFLFGHYLEQR
                     TLNQTRSAIKELTEMAPESALKQMDNGEFEEVEVDDIDEGDILLVKTGAKVPVDGIVL
                     TGEGHINEASITGEAVPVNKLADAEVFAGTILENGTIQIRADRVGEDTTFGKIIELVE
                     EAQDSKSEAERFIDRFSKYYTPAVLVLAIVVWAFSQNIELAITILVLGCPGALVIGVP
                     VSNVAGIGNGARNGVLLKGSEVIQDFSNVDTIVFDKTGTLTVGNPTVAATELYEKDPA
                     ETLGYLASVERESDHPLAKAVLNQIGETNFYPVEGTEVVKGGGIVSSVAGHRVAVGNV
                     ALMEKENVTLSKKVQKDIKRFEQHGNSLVLTAVDGELKVLMGIRDQVRPGVKANLQEL
                     KDLGVKNLVVLSGDNQGTVDVVAGELGLTEAHGHMLPEDKSAYIGKLQQRGQIVAFVG
                     DGVNDSPSLALADIGIAMGSGTDVAIETSDVVLMNSNFSNLPHALGLVKATANNMKQN
                     IVISIGVVLVLLTSVFFSEWMNMSIGMLVHEGSILVVIFNGMRLMKYKLKGRKEQKEV
                     SAPAEKVKIS"
BASE COUNT         1957 a         1005 c         1351 g         1699 t
ORIGIN      
        1 ccgcaaggaa cagttccaaa atgaagagtc gatggaacgt ttcttagtct catcttttga
       61 tacttacaac caaaaattcc taggtcgcag tcataaaggc ttccaacagg ccgaaggcga
      121 gctcgaacaa atgctgagtc aaccgatgga gaattagaaa caatctacag tagggaagaa
      181 ccatttacac aaaattattg acgctcccaa gctaaaactc ctattaatcg atctactaaa
      241 aataaataaa aaaattcttt tcaattttta tttatggtgt gacataaata aagtaaagtt
      301 attcttctag gttaatctta ttaatgctct ttctcactag tgttgcatgc aacactagtg
      361 agaaagagta ttaaatatac tgcaaggtaa gacatttggt aattgaagaa taacaagcaa
      421 aaaaatagca gtcaggtctc ttgacaaagt atagttgcta ttctttttta ggccctactt
      481 taatgaaatt tttgtttgga gggaacgact tcattccttt gattatttca gttgaattta
      541 atagagaagc ctgtcgtcta ttaagaaata cttttcttga tgagcgtcaa tttttttaac
      601 ggaaatctat cctatactaa attcagtaaa gaaaaaggag gaatcaaaga aagctatttt
      661 aaacaaacca aaattatagg aggaataaat catgactgaa aataaagcta ctcaagaaag
      721 aacaccccaa gaaagattgc aagaagaaca agaacacaag gaacacgttc atcatacgaa
      781 aattaatgca gcggccattg cggatcatct tttaggcaac atgcatacat tacatgtgaa
      841 actgcaccaa tatcactggt atgtaaaagg agccaatttc tttacgttgc atgaaaaatt
      901 tgaagagttg tataacgaaa atgaaaaatg gttcgacaaa cttgcggagc gtctaattac
      961 ttctgggcat aaacctgctt cgacaacggt tgaatttgaa aaatattcga tgctttcgga
     1021 agatgcagtt aataaatacg ctaaagcaga agacatggtt gaaaatatta tcgaggactt
     1081 cagaagcact cgtgacctaa ctattcgcgc gattcgttta gctcaagacg agggtgacga
     1141 tgctttagaa gatacattga ttgcttttaa aaatgactta gacaagaaca tttggatgct
     1201 gcaagcgttc attggcaaag aggcattgga agacgacgat gcgtttgatg aggatgaaga
     1261 ttaggttttt cgtaaacata catttaaaaa ttaaagattt tggggttggg actctttcca
     1321 gctcccattt ttttgccgtt aaaaagactt ttcttgatga ttgtcaattt ttttgaggta
     1381 gggggccttt atactaaata taggaaagtt aaaagaagga caataaactg ataaagggga
     1441 agaacgaatt gactcaatac acccaaccac atgcagcagg agaccactca gcttgtatcc
     1501 gcttagtccc gatattcaat catttggagg aaagctcgat ggatcgcatc gctggaaaag
     1561 ctcacacgaa gcactatcaa aaaggggagt ttctgtttcg ctcgcaagaa aaagatgatg
     1621 ccttgtatat tgtgaatcgc ggaaaagtcc gcatctatcg cttgtctaat tctggcaaag
     1681 agcaactagt tcgtattttg aatccgggtg actttacggg agaatggacg ttgttcaatc
     1741 ctgatgctgt gcacgaggaa tacgcagaag ctactcgaga tacgtctgtc tgcatgattc
     1801 aacaacatga tgtacaagag tttctaaaag agtatcatgc aatttcgtta aagctgctag
     1861 gagagatgtc acagcggtta gaaagctccg gacgtcaaac tgcacaagta gccgtggaga
     1921 gtgtcattac ccgtttagtt ttgtttttgg cagaaaatgt gggacctgaa atgggtaact
     1981 ctcccaccat caccctgccg atggcaaaaa aggacattgc ttcctattta ggaacgacac
     2041 ctgagaccat cagtcgcaaa tttggagaat tggaggacga gggattgatt caacagctgt
     2101 ctgggaaaag gatcaagatt caggatttag atgatttatt gctttacagc gaataatcgg
     2161 acacggaaaa gtggcgtatt acgaagcgag tgaccaggga gctaaaagtg aattcactgt
     2221 ttggtttccc tggaggttag ccggaatctt gttcagcatc accatgacta gaatgacgcg
     2281 aaaaattgaa gaaatggagc aaatagaatg ggattcgtat taggcgtttt gaatccccat
     2341 gtggtggaga ttggttctgt tgtgattcat tggtatggag tcattattgc agcgggcata
     2401 ttagcggctc cccagttgag caccaaggaa gcaaagaaaa aaggactgga agaggatacc
     2461 attatcgata tggccttatg ggcaattccg attggactcc tcggcgctcg gctttattat
     2521 gtgttatttg aacttggtta ttatctgcaa aatccaggac aaatccttac gatttggaat
     2581 ggcggtattg cgatttatgg gggattgatt gcaggagggg gtacgctata ttggtatgca
     2641 aagaaaaaag gtacttcttt ggcactcgtt cttgatattt tagcgcccaa tgtattgtta
     2701 gcccagtcca ttggtcgttg ggtcaatttt atgaatcagg aagctcatgg tgggccggtt
     2761 actcgtcaat tcttggaaaa tctttattta cctgaattta tcattgaaca aatgaatatg
     2821 aatggtacct actaccatcc gactttttta tacgagtcat tgtggagttt gttgggcttt
     2881 gtcctcatcg tcactatacg gaaccgaaaa cagcttttgc gtcaaggaga agtcgctttg
     2941 agttacgttt tgtggtactc agtgggtcgg ttcttcattg aaggtatgcg aacggacagt
     3001 ttatggattg gcgagtggat ccgcgtttcg caagccttgt ctctggccct gtttattggc
     3061 gcgattatag tatggtttat acgcagacaa gattatccac caatttctta ttattctgat
     3121 ggcaataagg ggcagaaaaa gactattaag atggtgaatt gagcaaaaag ataaaaagaa
     3181 gtgcaaaact tgatatgcgt caattttcgt ttgctaaaag ggagctatac tacagttgta
     3241 aagaggaaaa ggcagtgttc attaatctgc tgccgataaa ataaaaacac ataaaggaga
     3301 taggaattat ggaaaaagct atattgcaat tagaaacgtt gtcttgtcca agttgtatgc
     3361 aaaaaattga aggtgccgta aaatcagtta acggtgttga taaagacagc attaaagtat
     3421 tattcaactc cagcaaagtc aaagccaatt ttgattcagc tgtcacttcg attgaagaga
     3481 ttcaaaaagc tatcgaaaac gtaggctatc cggtccttaa agcaaaagtc aaggccgctt
     3541 aacagattaa atgaactagg caattctgga agaaaagcta tttttccagg attgcttttt
     3601 ttgttgcaat ccgattcgag gtgcttagag acgataaaag agaactttct aaacttgatg
     3661 tcagtcaatt aattacttag gcaattatct tatactgtgt ttatcaataa aagagaaatt
     3721 aaatttggag ggaaaaagca tgcaacaata tatattaagc aagaaaaatg ttatcaccgt
     3781 catcagtgga ttgttaatcg tactcggttt cttcagtcac tttgttttag aaaacgtagg
     3841 actttcagag tggtctttga tcatcgcctc tgtctttggg attacgccaa ttgccattca
     3901 agcgtttcaa gcaatgaaag tcaaagtcat cagtattgat gtcttagtca gtattgcagc
     3961 gattggtgcg ctttttattc aaaattatga agaatcggct attgtcacat tcttattctt
     4021 attcggacac tacttggaac aacgaacatt gaaccaaacg cgatctgcta tcaaagaatt
     4081 gactgaaatg gcacccgaaa gcgccttgaa acaaatggat aatggcgaat ttgaagaagt
     4141 agaagtggat gatatagatg aaggggatat cttgctcgtt aaaacgggtg cgaaagttcc
     4201 agtagatggc atagtcctta cgggtgaagg gcatatcaat gaagctagca ttacaggtga
     4261 agctgttcca gtcaacaagc tagctgatgc agaagttttt gcaggaacca ttttagaaaa
     4321 cggaacgatt caaatcagag ctgaccgagt tggtgaggac accacatttg gaaaaattat
     4381 tgaactcgtg gaagaagcac aagattctaa atccgaagcg gaacggttca ttgatcgctt
     4441 ttctaaatac tacacaccag ctgtcttggt tctggcaatt gttgtatggg cgttcagtca
     4501 aaatattgag ttagcaatca ccatcttggt tctaggttgc ccaggcgcat tagttatcgg
     4561 agtgcctgtc tcaaacgttg ccggtattgg gaatggtgct cgtaacggtg ttcttttaaa
     4621 agggagtgaa gtcattcaag acttcagcaa tgtggataca attgtatttg ataagacagg
     4681 tactttaact gtcggaaacc caaccgttgc agcgactgaa ctgtatgaaa aagatcctgc
     4741 tgaaacgctt ggctatctgg ccagtgtgga acgggaatca gatcatccat tagcaaaagc
     4801 ggtcttaaat caaattggag aaacaaactt ttatcctgtt gaaggaactg aagtcgtgaa
     4861 aggcggcgga atcgtttcaa gtgtagctgg acatagagtg gctgttggga atgtggcctt
     4921 gatggaaaaa gaaaatgtca ctctcagcaa aaaggtgcaa aaagatatca aacggtttga
     4981 acaacatggg aactctctcg ttttgacagc ggtcgacggt gaattaaaag tactgatggg
     5041 cattcgcgat caagtccgtc cgggtgtgaa agctaatttg caggaattaa aagacttggg
     5101 cgtgaaaaat ctagtcgttc tttcaggaga taaccaagga accgttgatg tggtcgccgg
     5161 cgaacttggt ttgaccgaag ctcacggcca catgttgccg gaagacaaat cggcttatat
     5221 cggaaaactt caacaacgtg gtcaaattgt agcctttgtt ggagatggcg ttaacgatag
     5281 tccgtcctta gctttagcag acattggaat cgcaatggga agcggaacgg acgtagcgat
     5341 tgaaacatcc gatgtcgttt taatgaactc gaacttcagc aacttgcctc acgcattagg
     5401 attagtaaaa gccaccgcaa ataatatgaa acaaaatatc gtgatttcaa ttggagtagt
     5461 gttggtcttg ttgaccagcg tcttcttcag cgaatggatg aatatgtcta tcggaatgtt
     5521 ggttcatgaa ggtagtatct tggtggtaat tttcaatggc atgagattaa tgaagtataa
     5581 gttgaaaggt cgaaaagaac aaaaagaagt atcagcacct gcagagaaag taaaaatatc
     5641 ttaagaataa attagaagag gctgagacaa tagtccatcc ttgaaatgac aaaagcggaa
     5701 actcagattt ttaaatctgg gattttccgc ttttttgagt tcttgaaaaa aaataagagt
     5761 atagagtatg ttctttataa aattaatttt cgtgtaaatg attaaaaaat ttaataataa
     5821 aatttaaatt cctattaagt tgtaacacat gaaattgctt ataatagtgt attgaaatta
     5881 aggtagattg acaaacgagt gatcaaatag atatactacc ttcaaacgac cgtttgaatg
     5941 tgaggtgaga tgaatggaaa aagtttgcca agtgactatc gtacataaag aaaaagtaaa
     6001 tgtaataaaa aa
//
LOCUS       DACEUZ010000128         5923 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.128, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000128 DACEUZ010000000
VERSION     DACEUZ010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5923
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.128"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(74..1168)
                     /locus_tag="IXG44_002248"
     CDS             complement(74..1168)
                     /locus_tag="IXG44_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAQ1658950.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            1357..2118
                     /locus_tag="IXG44_002249"
     CDS             1357..2118
                     /locus_tag="IXG44_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ1658951.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(2233..2673)
                     /gene="fabZ"
                     /locus_tag="IXG44_002250"
     CDS             complement(2233..2673)
                     /gene="fabZ"
                     /locus_tag="IXG44_002250"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAQ1658952.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            2893..3648
                     /gene="fabI"
                     /locus_tag="IXG44_002251"
     CDS             2893..3648
                     /gene="fabI"
                     /locus_tag="IXG44_002251"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HAQ1658953.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            complement(3758..4771)
                     /locus_tag="IXG44_002252"
     CDS             complement(3758..4771)
                     /locus_tag="IXG44_002252"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HAQ1658954.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            4936..5856
                     /locus_tag="IXG44_002253"
     CDS             4936..5856
                     /locus_tag="IXG44_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HAQ1658955.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
BASE COUNT         1806 a         1179 c         1063 g         1875 t
ORIGIN      
        1 tataaaaaaa caaaataaaa aagcagaaat aactggctat ttctagcgga ttatttctgc
       61 aataactaat tatctatttt ttattttcaa ttaatttaaa gcttgaccat ggtttcaaaa
      121 agtcaaggag gaataattcg tcatcggaga ttcttcctac aacattgaca cgtccgtcgt
      181 ttttcatatc cttcaaggcg atttgtgttt ctcctttgta ttgaccgtac ccttcattgt
      241 caatcagtac atccccatga tgcatatctc gcgtattatg aggcgggaaa tctttatctt
      301 tgtagtatac acgagtcatc gttgaacgaa ggatatattc tgagcgatcc cctcgataac
      361 tatgaaggtt atcaaacaag cagattcgtt cgttttctgt aataccttct tccgtatcta
      421 ctttcaatgt tggataatcc gcgttaaatg cttctgccat ttctttcaat tcttcttcac
      481 ttgcgtacgc atttccaata gaaatatcat caatcaatcc tgtaaggatt aaatgcttca
      541 cttgtgtcgc aatttcaaga tctcggtggt cttccagcgt gcacaagcca tcttgcgtag
      601 gccaagggcc aaactcagca gattgcgaat tgacgaatgc catcgtattc aaattatatt
      661 ttctgaattt ttctgaacaa aaaacaaagt gttcgtatcc taaaccagag tatcgatgag
      721 gataaaaatt atgagaacct aataaattat ccgtgtttgg cgaataactc atgatgttgt
      781 caacgtaatt cgtgcccgaa ctcatattga tctcgatttt gataccaaaa ggattgcgcg
      841 tcatttttgc ttcttctgct cctgtgaatc cgatatctaa gcggaccccg taggcaccca
      901 tcttatggaa aaaagataag tcatcgtaag aaatctccaa ctgttcaaat aacgctggat
      961 tgatatcaac cattacttcc atgcctaggc tattcgcgta atcaactact tctttaaatt
     1021 ctgaaagaac tttttcttta tcatcattga tctgcagcaa actggtaaaa acccgtttat
     1081 acccatactt atgagctaaa tccaagtacg ctttgtcctt ttcaaatgtt gaccgttctg
     1141 gataaatcga gattcctaat tttcccatat taccatttcc tcctgcatct tttcgttttt
     1201 gtatccactt acattatagc aaacacaaag taaatggtct acaccttttc tttttctttt
     1261 aaaataaatg ataaattcca ataatttaag taggtatttt ccctctattt tgttatcatt
     1321 aaagataaca aaatagtaaa ttggaaagga acagttatga agaaaaataa agttatttta
     1381 agtattagtt tagcagcagc attgctgttt gctgctggtt gttctgcaaa caacacgact
     1441 tcttctagtt cgtctacaga aagttcaaaa agtacatcta ctgcggaaag cacgactgcc
     1501 agtagcgtcg atttgaacgc attggaatta ccacaattga cgaaagatgt cgccgaaaat
     1561 gaagatctgg tcgaaatggt gacgaataaa ggcacaatca aaatcaaatt atttcccgaa
     1621 cacgcaccaa aagcggttga aaactttatg acacatgcaa aagacggtta ctacaacgga
     1681 ctgactttcc atcgcgtcat caaagatttc atgatccaag gtggcgatcc taaaggcgat
     1741 ggcactggag gggaaagcat ttggggagaa ggatttgaaa cagagatcaa caaccacctt
     1801 tataatataa gaggtgcgtt atctatggct cgctcacaag atcctaacag taacggcagc
     1861 cagttcttta tcgttcaaaa tacagatgat atgcatgatg gtcttttaaa agacgactat
     1921 cctcaagcaa tcatcgacgc ttataaaaac ggcggttatc cttcactaga tggccattat
     1981 actgtcttcg gccaagtgat tgaaggaatg gatgtcgtgg atgctattgc aaatatcgaa
     2041 acagatagtt cagacaaacc aaaagaagaa gtaaaaatcg ataaaatcaa tatactgcaa
     2101 gaagcaaaac aaaaataaaa aaataacgtc tgaatcctgc atagatgaca acgtatgcta
     2161 ggattcaggc gtcttctttt tacataaaaa gcaagtaagc ttccttttgt tttgctatat
     2221 caggttttat agtcaagcat tttggttgac gatgaacgtg aactcgcact cacagacttt
     2281 tttatcatct acatatgctg cagataacgc tgtaccgact gatcccttca ttttggtgat
     2341 ttcaaaatga catttcagca catctcctgg aacaactttt tgacggaatt tggccttatt
     2401 gatcccaccg atataagctc tttttcctag aaactcttct gattttagga taagtatcga
     2461 acctgcttgt gccagtgttt ctagaatcaa cgcacccggc atcactggat tgtcaggaaa
     2521 gtgaccttgg aaaaaatctt cattgattgt tacatttttt gtcgcaacga tcttttttcc
     2581 tggttctaaa gtatctacat aatcaatata acaaatagga taacgattag gaatcaattc
     2641 cattacttca gtagcagata atacgtaaga cataattaaa ctccttcttt caggcaattg
     2701 ccattttcat tactttgact atcaaagtat catattccta aatatttagc aatctttttt
     2761 taatttaaga aaaaatagtt tgacaatcaa aacattttga ctaataatta agaaatcaag
     2821 aagttatttt agctattatt ctctattttt ttacgactat caaattttta tcagaaacaa
     2881 aggagagtca ttatgtcatt tctagaagga aaaaaaatcg tcattatggg cgtcgctaac
     2941 aaaaaaagca ttgcttgggg ttgtgctgaa gcaatgttga aacaaggggc agaaattatc
     3001 tatacttacc aaaatgaacg aatgaaaaaa tcattagtca aattggtcgg cgaagaagca
     3061 ttcactgtag aatgtgatgt ttctagcgac gaaagcatcg aaaaagcaat gaatgccatc
     3121 ggtgaatatg ctggggagat ccacggcctt gtccatgcag ttgcttacgc caacaaagaa
     3181 gaattgtccg gaaatgtgtc tgatatttcc agagacggct atcaattagc tcaagatatc
     3241 agcagctact ctttaattgc tgtatcaaaa tacgcacaac cgtttcttgc accaaacagc
     3301 gggatcgtta gcatgactta tctaggttca gaacgcgcaa tcccgaatta taacatgatg
     3361 ggtattgcga aagcttctct tgaagctgct atccgttatt tggcagctga gttctcgcct
     3421 aaaggaattc gggtcaatgg aatctcagct ggggcaatca aaacattagc tgtcacaggt
     3481 gttaaagact atcaaaaact gatccagtta tctgaatctc gtacgcctga tcagcaaggt
     3541 gtgacgatcg aagaagtcgg taatgcttgt gctttcttaa tcagcccact ttccagcgga
     3601 atcatcggtg atattatcta cgttgacaaa ggtgtccatc tttcataaac ttttgttgca
     3661 ttctttaaag taaaacatat aaaaatcagg aaaaaacttt ttaaagtctc ttcctgattt
     3721 tttcttttta cgtttttttc aactcattac tttgaaatta ttccacgact tcaatccagc
     3781 cttctggtgc ttctatgtct ccaaattgaa tacctgtcaa ttcatcatac aaccgttttg
     3841 ttactggtcc tacttccgtt tcactataaa atacatgaag attgttattg tgataaatcc
     3901 caccgattgg agaaataaca gccgctgtac cacatgcacc agcttctgca aattgatcta
     3961 gatcatcaat gaaaatatct ccttcaattg cttgcagtcc tagtcgttct tcagctaact
     4021 gcaacaagga atatttcgtg atacttggta agatagaggg cgattgcgga gtgatgaatt
     4081 ttccgtcttt tgtaattcca aagaaatttg ctgcacctac ttcttcgatt ttcgtatgtg
     4141 tggctggatc aagataaaca caatctgagt aatccagttc ttttgcttct gttcctggaa
     4201 gtaaactcgc tgcgtaattt ccgccgactt ttgctgcacc agtcccctta ggagcagcac
     4261 gatcgtaatc agatacaata aaattcgttg gttttagtcc acctttgaaa tacgctccta
     4321 ctggcgtaca gaagacgaca aataaatatt ctggagctgg atggacacca atgttttcac
     4381 cgaccccaat cagtaaaggg cgcaagtata atgtagcgcc tgttccatag ggcggcacaa
     4441 aatcttcatt tgcttttacc acttgcttga cagcatcaac gaacatcttt tcagggacca
     4501 ttggcatcag caatcgtcta gcactttgat tcatccgtcg actattttgt tcaggtcgaa
     4561 agagattgac cttgccgtct tttcgacgat aagcttttag accttcaaaa cattgctgac
     4621 cataatgaag cgctggagag ccttcatgaa tcgtcaggta attatcttct gtcaattctc
     4681 cttcttccca ttgtccgtct ttccatttgg caataaaacg ccaaggagtt ttgatataag
     4741 aaaaacctag attgttccaa tcgatttgca tatacttacc accttcgtta ttttctccac
     4801 attttatcat tatccatatt atttgtcatt ttattttcag cttttttcct tcattatagt
     4861 tatttttgct atttcctttt ttgaaaactt cttggtacaa tacagaaaga atcaaatttt
     4921 agaaaagagg attttatgtt ttcattatta gcaactgccg gtacgacaga ctcttctggc
     4981 gagacattga cagatcaagc tgtcagcaaa gtaaatgcgt ttcagcggtt ttgggacggg
     5041 attgattggg acgccatttt ggctgccttt atccaaaaaa gtttatatct gatttttttg
     5101 atcgtccttt tttggattct tcaccgtgtg ggaaaatatc tgattgataa gtcttttaat
     5161 cagtattcta aaaaagccat tctaaacgag agccgcttaa agactttaca tagtctttta
     5221 aatactattt ttcattatac ccttggtttc ttttttatct atgcaatttt atctgctatc
     5281 ggtgtgccga tcggatcact tttggctggt gccggaattg caggtgtggc aatcggtctt
     5341 ggtgcccaag ggttcatgag tgatgttatt accggatttt ttattatcat ggaacaacag
     5401 atggatgttg gggactacat caagctagca aacttaacga ttgaaggaac cgtcgtatct
     5461 gttggtatcc gtacccttca gctgaaagca acggatggta ctgtccattt catcccgaat
     5521 cgaaacatca ccacgatcag caatctctct cgtgcaaata tgcaagtact tttagatatt
     5581 cggattgttc ctgaagaagg atatgacaaa atctatgaga tcattgatcg agtaaatcgt
     5641 acattagctg aaaaatacca agaagagctc caaactgaac cttctatctt tggattagtg
     5701 gatcttggaa acagcaattt tgcgattcgt acggtctgct acgcattgaa cggaaaacaa
     5761 tatgccttaa aagaagagtt cttgagctcc tatgtcaaag aattgacagc ttctggtttc
     5821 acgattccga acagtccaat tgctgtcgga aaataatttg gacaaacgat gcaacgaatc
     5881 tcactaaaaa gagcgtggga caaaagtaaa gaacactttt gtc
//
LOCUS       DACEUZ010000129         5873 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.129, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000129 DACEUZ010000000
VERSION     DACEUZ010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5873
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.129"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            640..1374
                     /locus_tag="IXG44_002254"
     CDS             640..1374
                     /locus_tag="IXG44_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1658956.1"
                     /translation="MQNTFQSKSLYYQLVEILKQRIENSMVAHDKLPSERELTSFYGV
                     SRTTVRLALQELEKMGYIYRQHGKGTFVSELRTNATDLAGAYSFTEQMRAMGKHPETK
                     ILAFKKIEATKYLASHLNVQLGEPLFQLDRLRLADDLPMMIEKSYLPVKLFMSLSQEI
                     LFLKPLYNLFSENFNQIVRLADEEFYASIVQKEDSELLNIPEGSPVLHLVRSTYNLEN
                     EVIEFTLSVARADKFHYKIRHLRSTN"
     gene            1390..2559
                     /locus_tag="IXG44_002255"
     CDS             1390..2559
                     /locus_tag="IXG44_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ1658957.1"
                     /translation="MFMFSKEELRKLGAENTTREIRQQPELWHETFDRYQETVADLDR
                     LFTDAKQHAGERPIRVIFTGAGTSQYVGDTLIPFLTSQGNTNQFIFQSIGSTDIVATP
                     KEYLIPDETTILVSFARSGNSPESLAAVKMANQLVNKIYHIVITCAPDGLLATQMPTK
                     KENLVLLMPNKANDAGFAMTSSFSCMTLTALLIFDQASNQQKERYVHQLSRLGEEVIQ
                     RESEIQGYLASDFHRIIYLGSGSLGGLTREAQLKVLELTAGKIATVFDTSLGFRHGPK
                     SFVNEQTLVFLFVSNDSYTQKYDVDMLEEIKNDGIAQRVVGIGQKGSLFSGDSFELVA
                     DSLLPDGYLALADIMVAQTIALLCSVKVGNTPDTPSPSGTVNRVVKGVTIYPYEK"
     gene            2575..3570
                     /gene="lacD"
                     /locus_tag="IXG44_002256"
     CDS             2575..3570
                     /gene="lacD"
                     /locus_tag="IXG44_002256"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAQ1658958.1"
                     /translation="MLEWSENKRQAMERLSTPEGIITALAIDQRGALKKMMSALGMEA
                     TKEKIEQFKTLISQELTPYASAILLDPEYGLPAAQKRAAASGLLLAYEKTGYDQTVPG
                     RLPDLLSDCSVLRLKELGADAVKFLLYYDYEEAEEINRQKQVFVERIGSECMAEGLPF
                     YLELLSYDAKNVDTKSLSYAKAKPRKVIEMMKEFSKTQYKVDVLKVEVPVNMSFVEGN
                     ATGEIAYTRQEAAAYFKEQSDATDLPYIFLSAGVSAALFMETLRFAKHAGARFNGVLC
                     GRATWKDAVAPFVEKGEAATLEWLTEQGTQNIRQLNEVIRETAIPWYEKVSESTC"
     gene            3649..4575
                     /locus_tag="IXG44_002257"
     CDS             3649..4575
                     /locus_tag="IXG44_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HAQ1658959.1"
                     /translation="MNKRLLDYSASDFLKAKPMELKQAILASEGRTIMAENVPSSCPY
                     MGKSVTNAELERAAGADLILFNALDLFNPQISGVPLTLKENPIIWIKKALGRAIGVNL
                     EPVDKEVLMSENRTEISSGRIVSPETLKKANELGFNFICLTGNPGTGVSNRTIEKAIT
                     LSKSIFNGLIIAGKMHGAGVDEPIMNLDIAKRFIKTGVDILLVPAPYTVPYFMESDLR
                     GIVDYIKDFNRNKSIEEKVLVLTANGTSQDSSDQETIKKIALASKACGADIQHIGDSL
                     NGICLPENIFALGQAIRGYRHQIIMLGKSNYR"
     gene            4594..5844
                     /locus_tag="IXG44_002258"
     CDS             4594..5844
                     /locus_tag="IXG44_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1658960.1"
                     /translation="MIDKVQKIAAKISNQRHLLALRDSMAAVMPLIIIGSFFMLLANF
                     PIEGFTKWLSEIGVQSFLNKASDSTFGIIGLAVTYSLGHNLAKTYGVDSLSAGLLSLS
                     GYVLLTPLLDADGSIGFPTKYFGTSGLFVGIIVALVSTEIFRWFVLKNITIKMPASVP
                     PNVSRAFVAIIPGFFVVLLWFIVVVICYKLGIENVHALIADTLAKPLSLLTKTLPGII
                     LVILIQCFFWMFGIHGAQVTGPIIEPLLLQNSDVNRIAYQAGKELPNIITYEFLYNFV
                     FSGGAGCLFALAILTFFFSKSQENKTLGKLSIAPVSFQVAEPLLFGFPTILNIKMLIP
                     FVTAPVVTTLITYFSMSMGLVAKPVGATIPWTTPPIIAGFLASGGRISGAVIQVITIA
                     INVLIYYPFFKLDDNAKLKSEKND"
BASE COUNT         1967 a          955 c         1201 g         1750 t
ORIGIN      
        1 tttgaaaaat gctatttcga ggtagaagga tattatataa gcacggttga ctaaacgaag
       61 tgataataaa aaaagttatt caagaataag aaaaattatg atacgaattc agataaactg
      121 gagaatacag agacctttaa aagggataag aaacaacata aattcctctt aagagagaac
      181 gaaaggacaa agccagcaac ttgatacacg agctagtatt agaggaattt attctcatag
      241 actccctttt gaatacgttt caactttaat tttcatgttc ataaatgatt tgaaaccaaa
      301 gcaaatgcgt tgcacttttc ctccgtcttc cgaaaatcgt tcaattcact ggtaagtagt
      361 catttgatag tttgaggcaa attgttgatt gatgaagggt aggagctaaa aaaagatacc
      421 agttgaagag agaaaaagaa aaaaatagag ggtgttcttg aatgaaaaaa gttggaaaat
      481 tatttttcat ttccttttat tcctattaaa gtcagtgatg tcaatcatat taacaagact
      541 aaaaatgaaa ataaaaaaaa gattgttctt agtattgact cataaaaaat accactacta
      601 tacttacatt gtggttatag ccagttagga gagacatcga tgcaaaatac ctttcaatca
      661 aaatcacttt attatcagct ggtagaaata ttgaaacaac gaatcgaaaa cagtatggtc
      721 gctcatgaca agctgccatc agaaagagag ttgaccagtt tttatggcgt tagtcgcacg
      781 acggtgcgac tagcattgca agaactggaa aaaatgggct acatttatcg acaacatgga
      841 aaaggaacct ttgtttcgga attaaggaca aatgcgactg atctagctgg cgcatatagt
      901 tttaccgaac aaatgcgggc aatgggcaaa catcctgaaa ccaagatatt ggccttcaag
      961 aagattgaag caactaaata tttagcgagt catctgaatg ttcaactagg tgagccttta
     1021 tttcaattag accgattgcg cttagctgat gatttgccaa tgatgatcga gaaatcctat
     1081 ttgccagtga agctatttat gtccctttct caagaaattc tatttctgaa acctttatat
     1141 aatttgtttt cagagaattt caatcaaatc gtacggttgg ctgacgagga attttatgcc
     1201 agcattgtcc agaaagaaga tagtgagttg ctaaatattc cagaaggatc accagtgctc
     1261 catttagttc gctcaaccta taatctcgag aacgaagtga ttgaatttac attaagtgta
     1321 gcgcgggctg ataaatttca ttataagatc aggcatctgc gttccactaa ctgaattgga
     1381 ggaaagaaaa tgtttatgtt ctcaaaagaa gaactacgaa agctaggagc agaaaatact
     1441 acccgagaaa ttcgacaaca accggaactg tggcatgaga cgtttgaccg ttatcaagaa
     1501 acagttgcag atttagatcg tttgtttacg gatgcgaaac aacatgctgg agagcgacca
     1561 atccgagtca tttttacagg ggccggaacc tcgcaatatg tgggagatac actaattccg
     1621 tttcttacta gtcaaggaaa tacgaaccag tttatttttc aaagtattgg ttcgaccgat
     1681 atcgttgcta cgccaaaaga atacctgatt cctgatgaga caacgatctt ggtctcattt
     1741 gcccgtagtg gaaatagtcc agaaagtcta gccgcagtta agatggccaa tcagctggtc
     1801 aacaagatct atcatatcgt gatcacctgt gcaccagacg gattattggc tacacaaatg
     1861 cctacaaaaa aagaaaatct agttttacta atgccaaaca aagccaatga cgctggtttt
     1921 gctatgacaa gcagtttcag ctgtatgacg ctgactgcgc tacttatttt tgatcaagca
     1981 agcaatcagc aaaaagaaag atatgttcat caattaagcc gactaggaga agaagtcatc
     2041 caacgagaat cagagatcca agggtatctg gcatcagact ttcatcgcat catttactta
     2101 ggttcgggaa gcttaggcgg attgactaga gaagcgcaat taaaagttct cgagttaact
     2161 gctggaaaaa tcgcaaccgt atttgataca tctttaggat tcagacatgg tcccaaatct
     2221 tttgtcaatg agcaaacatt ggttttcctg tttgtcagca atgactcata tacgcagaaa
     2281 tacgatgtag acatgctgga agaaatcaaa aatgacggta ttgcccaacg cgttgttgga
     2341 attggtcaaa agggttcatt gtttagtggt gattcctttg aattagttgc agattcgtta
     2401 ctgccggatg gctatcttgc tttagcagat atcatggtag cccaaacgat cgcattactt
     2461 tgttcggtga aggttggtaa tacaccagat acaccatcgc caagtggaac agtcaatcgt
     2521 gtggtcaaag gcgtaacgat ttatccatat gaaaaatagg aaagagtgtg aagcatgtta
     2581 gaatggtcgg aaaataaaag acaagcaatg gaacgtctat cgacgccaga aggcattatt
     2641 accgctttgg cgatcgatca gcgaggcgcg ctgaagaaaa tgatgtcggc tttagggatg
     2701 gaagcgacaa aggaaaaaat tgagcagttt aaaacactaa tttcccaaga attgactcct
     2761 tatgcctcag caattttatt ggatcctgaa tacggactgc ctgctgcaca gaagcgtgca
     2821 gcagcgagcg gtctgctgtt ggcatatgaa aaaactggtt atgatcaaac cgtacccggc
     2881 cgcttgccgg atctgctgtc ggactgttca gttctgcgat tgaaagagtt aggtgcggat
     2941 gcggtgaaat ttttactgta ttacgattat gaagaagcag aggaaatcaa tcggcaaaag
     3001 caagtttttg ttgaacgaat tggaagcgaa tgcatggctg aaggactgcc attttattta
     3061 gagttattgt catatgatgc taaaaatgta gatacgaaat cgctttccta tgccaaagca
     3121 aaaccgcgca aagtgatcga aatgatgaaa gaattctcga aaacccagta taaagtagat
     3181 gtcttaaagg ttgaagtacc agtaaacatg tcatttgttg aaggaaatgc aacaggggag
     3241 atcgcctata cacgtcaaga agctgccgct tattttaaag aacaaagcga tgcaacagat
     3301 ttgccgtata tcttcttaag tgctggggta tcagcggcat tattcatgga gacattgcgt
     3361 tttgccaaac atgctggggc acgattcaac ggtgtactct gtggcagagc aacttggaaa
     3421 gatgcagtcg ctcctttcgt tgaaaaagga gaagcggcaa ctttagaatg gctgacagaa
     3481 caagggacac aaaacattcg tcaattgaat gaagtgataa gagaaactgc catcccatgg
     3541 tatgagaaag taagtgaaag cacttgttaa tgtttctaat atattagtag tgataagaga
     3601 tatgtgtgat tataatttat cattattgaa actgggagga tattttatat gaataaaaga
     3661 ttgttagatt attcagcttc agattttttg aaagctaagc cgatggaatt aaagcaggca
     3721 attttggctt ctgaaggtag aacaattatg gcagaaaatg tgccaagtag ctgtccttac
     3781 atgggaaaat cagtgacaaa tgcagaattg gagagagctg ctggagctga tttaattttg
     3841 tttaatgcac tagatctttt taatcctcaa atttcaggtg tacctttaac tctaaaagag
     3901 aatccaataa tctggattaa aaaagcttta ggaagagcta ttggtgtaaa tttagaacct
     3961 gtagacaaag aagttttgat gagtgaaaat cgaactgaaa tttcaagtgg tagaatcgtt
     4021 tctcctgaga ctttaaaaaa ggcaaatgaa ttaggcttta attttatttg tttaactgga
     4081 aatccaggaa caggagtatc taatagaact attgagaaag caataacatt atccaaatca
     4141 atttttaatg gattaattat cgctggaaaa atgcatggtg ctggtgttga tgaaccaatt
     4201 atgaacttag atatagcaaa aagatttatt aaaacaggtg tagatatttt attagttcct
     4261 gctccataca ctgttccgta tttcatggaa agtgatttgc gaggaatcgt tgattatata
     4321 aaagatttta atagaaataa atctattgag gaaaaagttc tagtactaac ggctaatgga
     4381 acaagccaag actcttctga ccaagaaact attaaaaaaa ttgcattagc atcaaaagca
     4441 tgtggagctg atattcaaca cataggggat tctttaaatg gtatttgtct tccagaaaat
     4501 atttttgctt tgggtcaagc aattcgggga tatcgtcatc aaattattat gttaggaaaa
     4561 tctaattatc gttaattaag gaggaaatga aagatgatcg acaaagttca aaaaatagct
     4621 gcaaaaatta gtaatcaaag acatctacta gctttaagag acagcatggc tgcagttatg
     4681 ccattaatca ttattggttc cttttttatg ttattagcaa atttcccgat agaaggattt
     4741 acaaaatggt tatctgaaat tggagtacaa tcctttttga acaaagcatc agattcaaca
     4801 tttggtatta ttggattagc tgttacctac tctttgggac acaatctagc aaagacttat
     4861 ggtgtagact cattatcagc aggattatta tctttgagtg gatatgtttt acttacacct
     4921 ttattggatg cagatggttc tataggattt ccaacaaaat actttggtac ctcgggtctt
     4981 tttgttggta ttattgtagc acttgtatct acagaaattt tccggtggtt tgtacttaaa
     5041 aatattacaa ttaaaatgcc ggcaagcgtt ccaccaaatg taagccgagc tttcgttgct
     5101 attattcctg gcttttttgt cgttctacta tggtttattg ttgttgtaat ttgttataaa
     5161 cttggaattg aaaatgttca tgctcttatt gcagatacac tcgcaaaacc actatcttta
     5221 ctgacgaaaa ctttaccagg aattatattg gttatactta ttcaatgttt cttttggatg
     5281 tttggtattc atggtgcaca ggtaacgggg ccaataattg aaccgttatt attgcaaaac
     5341 tctgatgtaa atagaattgc ttatcaagct ggaaaagaac ttcctaacat tataacttat
     5401 gaattcttat ataactttgt tttttctggt ggtgcaggtt gtttatttgc acttgccatt
     5461 ttaacatttt tcttttcaaa gagtcaggaa aataaaactt taggtaagtt atcaatcgct
     5521 ccagtaagtt ttcaagtagc agagcccctt ctatttgggt ttccaactat attaaatata
     5581 aaaatgttaa ttccatttgt aactgcacca gtagtaacaa cattaattac ttatttttca
     5641 atgagtatgg gattagttgc aaaacctgta ggagcaacaa tcccatggac aactccaccg
     5701 ataattgccg gatttttagc ttctggtgga cgaataagtg gtgcagtgat tcaagtaata
     5761 acaattgcaa taaatgtttt gatatactat ccattcttta aattagatga taatgccaag
     5821 ctaaaaagcg aaaaaaatga ttagaactac ctatatgttt ctaactgtag tta
//
LOCUS       DACEUZ010000130         5844 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.130, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000130 DACEUZ010000000
VERSION     DACEUZ010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5844
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.130"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            49..309
                     /locus_tag="IXG44_002259"
                     /pseudo
     CDS             49..309
                     /locus_tag="IXG44_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase"
     gene            472..1137
                     /locus_tag="IXG44_002260"
                     /pseudo
     CDS             472..1137
                     /locus_tag="IXG44_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000284571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element IS1182 family transposase"
     gene            1173..1418
                     /locus_tag="IXG44_002261"
     CDS             1173..1418
                     /locus_tag="IXG44_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ1658961.1"
                     /translation="MIDVNELLEEAIRETENLNDGEVFLVKDLFKGYVGNRIPRKDRL
                     LLGTLFLNHVSKMNGNLKAIEKTSSNQQRYKKTIDKF"
     gene            1521..2390
                     /locus_tag="IXG44_002262"
     CDS             1521..2390
                     /locus_tag="IXG44_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HAQ1658962.1"
                     /translation="MVDNIIKSVAEKLSSLSYIEGIVLGGSRARGTHTEDSDIDIGIY
                     YNSESFDINTINQFATKLDDEHRNNLVVPPGAWGDWINGGGWLVINGYHVDLILRDIK
                     RVEQIMKDTEHGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAETY
                     PNALQKSLVNFFMFEAGFSLMFVKANSGTDDKYYIAGHVFRIVSCLNQVLFACNNAYC
                     INEKKAIKLLETFEHKPEKYTEKVNHIFEVLGISLFECYDMTEKLYNEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            2371..3105
                     /locus_tag="IXG44_002263"
     CDS             2371..3105
                     /locus_tag="IXG44_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008766909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ1658963.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            3138..4046
                     /locus_tag="IXG44_002264"
     CDS             3138..4046
                     /locus_tag="IXG44_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HAQ1658964.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            4043..4585
                     /gene="sat4"
                     /locus_tag="IXG44_002265"
     CDS             4043..4585
                     /gene="sat4"
                     /locus_tag="IXG44_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HAQ1658965.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            4678..5472
                     /locus_tag="IXG44_002266"
                     /note="aph(3')-IIIa"
     CDS             4678..5472
                     /locus_tag="IXG44_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAQ1658966.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            complement(5580..5768)
                     /locus_tag="IXG44_002267"
                     /pseudo
     CDS             complement(5580..5768)
                     /locus_tag="IXG44_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913594.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5748..>5844
                     /locus_tag="IXG44_002268"
     CDS             5748..>5844
                     /locus_tag="IXG44_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ1658967.1"
                     /translation="MNRINRVTSILIQLQSKKIIPAKEIAQRFNIS"
BASE COUNT         2021 a          867 c         1269 g         1687 t
ORIGIN      
        1 aatttgtatc gataaggggt acaaattccc actaagcgct cgggacccct tgtaggaaaa
       61 tgtcctaagt gtggcaacaa tattgtatta aaaaaatcgt tttatggttg ttcaaattat
      121 cctgaatgta agtttacttt agctgaacat tttagaaaga aaaaactaac caaaacgaat
      181 gtaaaagaat tactggaggg aaaagaaacc ctggtaaaag gaatcaaaaa caaagagaaa
      241 aagccctaca atgccgttgt aaaaattggg gaaaagggat atattgattt tatctctttt
      301 tcaaaataaa catggggctg acccaaaagt agctcctata tgaaaaatag aggtctttcc
      361 atttagatag tagtggaaag acctctattt atcttttgaa agtgaataca aaaataaagc
      421 acactctgct ataatatagg tgattaatcc atttgagaga ggtgctttat catgtatcaa
      481 aattatacca taaaccaact ttgtttgcct atagatcttg aaattaaatt agaagaaaat
      541 gattttgctc atgccattgt gcagtttgtt gactctattc ccgatgaagt ctttcttcct
      601 tattatcaat ctatgggtag accccaatat catcctcgta tgctactttc cattattctt
      661 tgtgcctata ttcaaggggt ttattcgggc cgccaaatac aaaatatgct gatagatagt
      721 attcggatgc gctacttatc tcaagagcaa ttccctaact ttcgtactat taatcgtttt
      781 cgcgttcatc cgattatgaa cgatattcta gatcattcat ttgttcaatt tcgtgagcta
      841 ctggttcaaa gtggcttaat cagtggcagt gctctataca ttgatggtac aaaaattgaa
      901 gcggatgcga ataaatactc tttcgtttgg aaaaaaagta ttttaaagta taaggatagc
      961 cttgatcaaa aagcccttga aaactaccaa caaatggtgg aaacaaaaat cttacctgag
     1021 ttaatagatg atttaaagga tgaactttct atagaagaga ttgaaaggat tcgtcaatct
     1081 ttggaagaaa aggaaaatca attaattgaa gcgattgacc aaaccgaaac agtagaaggg
     1141 ggctgaccca ttattaataa ggagtggaca acatgattga tgtaaatgag ctattggaag
     1201 aagctattag agaaactgaa aatctaaatg atggcgaagt ttttcttgtt aaagatttat
     1261 tcaagggata tgtggggaat aggataccca gaaaggatcg acttctgctt ggaacgttat
     1321 ttttaaacca tgttagcaaa atgaatggta atttaaaggc tattgaaaag acttcatcta
     1381 atcagcagag gtataaaaag acaattgata agttttaact atcttttgtg aaattaaaaa
     1441 aattataaat atgttgagta gaagcaaaag atttggcttg gaatttttac agcagtgaat
     1501 cgataaaagg agcatgcttg atggtagata atattattaa atcagtagca gagaaattat
     1561 cctctctgtc ttatatagaa ggtattgttt taggtggttc acgtgcaagg ggcacccata
     1621 cagaggattc ggatatagat atcggcatct attacaattc agaatcattt gacataaata
     1681 ctattaatca attcgctaca aagctggatg atgagcatag aaataacctt gttgtacctc
     1741 ccggagcatg gggtgattgg attaatggcg gcggatggtt agtcataaac gggtatcatg
     1801 tggatttaat tttacgtgat attaaacgtg tggaacaaat aatgaaagat acagagcacg
     1861 gaattgttac tgccaattat cagactgggc atccccatgg ttatattagt gcaatgtatc
     1921 gaggagaatt agcgattagc aaaatactat atgctaagaa tgaaagctta tgcgaattaa
     1981 aaaaacaggc agaaacttat cccaatgctt tgcagaaaag tttagttaac ttttttatgt
     2041 ttgaagcagg gttctcttta atgtttgtaa aagcaaattc gggaacagac gataaatatt
     2101 atattgcggg tcatgttttt cgtatagttt catgtttaaa tcaagtgtta tttgcatgta
     2161 ataatgctta ttgtatcaac gaaaagaaag ctataaaact gcttgaaact tttgaacata
     2221 aacctgaaaa atataccgag aaggtaaatc atatttttga agtactcggt atctcacttt
     2281 ttgaatgcta cgacatgacc gagaagcttt ataatgaagt gaatgaaatt gtatcggaga
     2341 taaataactt tttaaacgag gagagttcag atgaaagaaa acaaatatga tgataatata
     2401 ttttttcaaa aatacagtca aatgagtcgc tcgcagaaag gactggctgg tgcgggagaa
     2461 tgggagactt tgaaaaagat gctacctgat tttaagggta agcgtgtgct tgatttagga
     2521 tgcggctatg gatggcactg tatatatgcg atggaaaacg gtgcttcctc tgtagtaggt
     2581 gttgatattt ctcataaaat gctcgaagta gcaaaaggaa aaacccattt tccacagatt
     2641 gaatatgaat gctgtgccat agaagatgtg gatttcccag aggagagctt tgatgtaata
     2701 ctaagttcgc ttgcgtttca ttatgtagca gactatgaga atttaataaa aaagatatat
     2761 aggatgctga aggctggtgg caatttagtt tttacagttg aacatcctgt ttttactgct
     2821 catggaacac aagactggta ttataacgaa aaaggagaaa tactgcattt cccggtggac
     2881 aattattatt atgagggcaa acggacagct atgtttttgg aagaaaaggt tacaaaatat
     2941 catagaacac tgaccacata tctaaataca ctgctttcaa atagttttat aataaatcag
     3001 attgtggagc cacagccgcc agagaacatg atggatattc cggggatggc ggatgaaatg
     3061 cgacgcccaa tgatgctgat tgtatcggca aaaaagaaga tgtaataata tagaaaaaat
     3121 aaacgaggag tatgtaaatg agatcagaaa aagaaatgat ggatttagta ctttctttag
     3181 cagaacagga tgaacgtatt cgaattgtga cccttgaggg gtcacgcgca aatattaata
     3241 tacctaaaga tgaatttcag gattatgata ttacatattt tgtaagtgat atagaaccgt
     3301 ttatatctaa tgatgactgg cttaatcaat ttgggaatat aataatgatg caaaagccgg
     3361 aggatatgga attattccca cctgaagaaa agggattttc ctatcttatg ctatttgatg
     3421 attacaataa aattgatctt accttattgc ccttggaaga gttagataat tacctaaagg
     3481 gcgataaatt aataaaggtt ctaattgata aagattgtag aattaaaagg gacatagttc
     3541 cgactgatat agattatcat gtaagaaagc caagcgcaag ggagtatgat gattgctgca
     3601 atgaattttg gaatgtaaca ccttatgtta ttaaaggatt gtgccgtaag gaaattttat
     3661 ttgctattga tcattttaat cagattgttc gccatgagct gctgagaatg atatcatgga
     3721 aggtcggcat cgaaacaggc tttaaattaa gtgtaggcaa gaactataag tttattgaaa
     3781 ggtatatatc cgaggatttg tgggagaaac ttttgtccac ctaccggatg gattcctatg
     3841 aaaacatatg ggaagcatta tttctatgcc atcaattgtt cagggcggta tccggtgagg
     3901 tggcggaaag gcttcattat gcctatccgg agtatgatag gaatataaca aaatatacca
     3961 gggacatgta taaaaaatac actggtaaaa ccggctgcct ggatagcaca tatgccgctg
     4021 atatagaaga gaggcgggaa cagtgattac agaaatgaaa gcagggcacc tgaaagatat
     4081 cgataaaccc agcgaaccat ttgaggtgat aggtaagatt ataccgaggt atgaaaacga
     4141 gaattggacc tttacagaat tactctatga agcgccatat ttaaaaagct accaagacga
     4201 agaggatgaa gaggatgagg aggcagattg ccttgaatat attgacaata ctgataagat
     4261 aatatatctt tactaccaag acgataaatg cgtcggaaaa gttaaactgc gaaaaaattg
     4321 gaaccggtac gcttatatag aagatatcgc cgtatgtaag gatttcaggg ggcaaggcat
     4381 aggcagcgcg cttatcaata tatctataga atgggcaaag cataaaaact tgcatggact
     4441 aatgcttgaa acccaggaca ataaccttat agcttgtaaa ttctatcata attgtggttt
     4501 caaaatcggc tccgtcgata ctatgttata cgccaacttt gaaaacaact ttgaaaaagc
     4561 tgttttctgg tatttaaggt tttagaatgc aaggaacagt gaattggagt tcgtcttgtt
     4621 ataattagct tcttggggta tctttaaata ctgtagaaaa gaggaaggaa ataataaatg
     4681 gctaaaatga gaatatcacc ggaattgaaa aaactgatcg aaaaataccg ctgcgtaaaa
     4741 gatacggaag gaatgtctcc tgctaaggta tataagctgg tgggagaaaa tgaaaaccta
     4801 tatttaaaaa tgacggacag ccggtataaa gggaccacct atgatgtgga acgggaaaag
     4861 gacatgatgc tatggctgga aggaaagctg cctgttccaa aggtcctgca ctttgaacgg
     4921 catgatggct ggagcaatct gctcatgagt gaggccgatg gcgtcctttg ctcggaagag
     4981 tatgaagatg aacaaagccc tgaaaagatt atcgagctgt atgcggagtg catcaggctc
     5041 tttcactcca tcgacatatc ggattgtccc tatacgaata gcttagacag ccgattagcc
     5101 gaattggatt acttactgaa taacgatctg gccgatgtgg attgcgaaaa ctgggaagaa
     5161 gacactccat ttaaagatcc gcgcgagctg tatgattttt taaagacgga aaagcccgaa
     5221 gaggaacttg tcttttccca cggcgacctg ggagacagca acatctttgt gaaagatggc
     5281 aaagtaagtg gctttattga tcttgggaga agcggcaggg cggacaagtg gtatgacatt
     5341 gccttctgcg tccggtcgat cagggaggat atcggggaag aacagtatgt cgagctattt
     5401 tttgacttac tggggatcaa gcctgattgg gagaaaataa aatattatat tttactggat
     5461 gaattgtttt agtacctaga tttagatgtc taaaaagctt taactacaag ctttttagac
     5521 atctaatctt ttctgaagta catccgcaac tgtccatact ctgatgtttt atatcttttc
     5581 taaaagttcg ctagatagag ttctatatta gagatgtaaa gagttttggt gaagaggttt
     5641 gtgagtatgg cttttataac cagggtgcaa tgagaagcac taacagcagc tagcttaaga
     5701 acgcatagga tatacttttt atggaagtca cagaggagga ataaaatttg aacaggatta
     5761 acagagtaac ctctattctt attcagctgc aatcaaaaaa aataattcct gccaaagaaa
     5821 ttgcacagcg atttaatata agct
//
LOCUS       DACEUZ010000131         5770 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.131, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000131 DACEUZ010000000
VERSION     DACEUZ010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5770
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.131"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(1015..1305)
                     /locus_tag="IXG44_002269"
     CDS             complement(1015..1305)
                     /locus_tag="IXG44_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1658968.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            1647..1943
                     /locus_tag="IXG44_002270"
     CDS             1647..1943
                     /locus_tag="IXG44_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1658969.1"
                     /translation="MQIGVLKWLQFTKQKGVHFPMQFLEPKNKNAKSVDWEISEQVRV
                     IVKQYAEYAERTESEAVDEFLLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            1950..3902
                     /locus_tag="IXG44_002271"
     CDS             1950..3902
                     /locus_tag="IXG44_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HAQ1658970.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(3974..4471)
                     /gene="dfrG"
                     /locus_tag="IXG44_002272"
     CDS             complement(3974..4471)
                     /gene="dfrG"
                     /locus_tag="IXG44_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HAQ1658971.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(4798..5490)
                     /locus_tag="IXG44_002273"
     CDS             complement(4798..5490)
                     /locus_tag="IXG44_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAQ1658972.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT         1894 a          997 c          917 g         1962 t
ORIGIN      
        1 catacttgca aagtaatact acaattccta acataatcgt agaatttaat aatttccata
       61 gcataaagtg tttagaaaag aacattaaga atagttctat aataattctt gaactccatg
      121 tcttataatg ttctatcgaa gcacccaaat agccaagttc atctaatcta cttgcataaa
      181 ctacgtcatc cgctctcata gtcggcatat acatattaaa gagtaaaaag ataccccaaa
      241 tccctattag taacaagatc agttctttgt tattcgttat ttttctatta tgtttgttca
      301 tttttatact ccttcttata tttcctaata gagtataaca tagctttaag cattacttaa
      361 agataactta aaagttatct tttaaatact taattgttaa ataccttatt tgatgcaaaa
      421 gaaatctacg atagtcaaca aaaaaatttt cattatatcc agaaaaattt ttcttagtta
      481 aggacctatg gattgcagta atgcagaatt cgtcgaataa actgccaaag acatacaatc
      541 atattgcttt taaaattgac gaacaagata tagattcgtt tgtctcaaaa atccaaatgt
      601 taggtttaac tgtcgaacca ggacgttctc gtgtgaaagg cgaggcttct tcaatttact
      661 tttacgacta tgataatcac ctcttcgagt tacataccgg tactctccag gaacgtttaa
      721 aatcctacaa ttccactacc tgagactagt aatgactagt ctctttttat ggcctctgat
      781 ccccccattg agcgaatact aatagggctt cgaattgatc tgttcaatct ttaagcaata
      841 tccctcaaat gagtttggaa agtttagtga ttcatcacaa atacttaaat acatgtagtt
      901 atatatagat tgaggttctt gtgagcgtat gctcgtgtgg gttcaaatcc catcgtctgc
      961 atccatcttt ccaaaacaag cggctaggac atttttgtct cagccgcttg ttttctagtt
     1021 tattctttca tcttgtcgga ccttttttct tgggtataga cttttctcat attctaccac
     1081 aattttgggt ttccctaaca catgatagga tctcacaaca aattgctttc tattattata
     1141 ataacctgta acgactaact tcatcccttc gtctacttca gctaggaagt tcaaactatg
     1201 ggaagcaatc aaacaattga ccccatttaa actgaaacgg atcaacggtc tggcactaac
     1261 ttgcaaagta cggatcttat ctactgttcc atattttttc tccataacct tcacctcaat
     1321 accattatac gaacatacat tccctttgta aagaagaaac tctgttttat ctttttgact
     1381 ttttgtctat tacctttctt aaaatcatat tggcacttcc tgcgaagatg aaggacatgt
     1441 actaaaaaaa agaggcagca gtatagattt aagtagttca gttttggagt acaaaaagtg
     1501 aaaatgtaaa cctaaatgag tatcaaattg caactgaatt tgatactcgt tttttattgt
     1561 taaatcaacg tttgttgtta atttacacca caaaatgtaa gttaaaatgt atataaaaat
     1621 gcgaacgtga aataattatt gtaggaatgc aaattggtgt gttaaaatgg ttacaattta
     1681 ctaagcaaaa aggggtgcat tttcctatgc aatttcttga gccaaaaaat aagaacgcta
     1741 agtctgtgga ttgggaaata tcagagcaag taagggtgat agttaagcaa tacgctgaat
     1801 atgcagagcg tactgaaagc gaagcagtgg atgaattctt attgaacatt ttagacgata
     1861 agaagtttat tgaatggatt gccaacaagc gaagtaataa gcggattgtt gaaaaaatgg
     1921 gtatcaaaga tagagtgggt tgatagacaa tgaccaagct taaaagatta gccacgaagg
     1981 aagatgaaat tgtagatgtt aaaattccga tttcaaacga agaattaaaa gatagggcta
     2041 aacaatatga tttattaact ccaaaacgat ttgcaacaag gtataacaaa atgttgttct
     2101 tgccaacgtc ttttaaatgg aacggttctg aatatcccat tcaatataat tactgcatca
     2161 atccgttttg ttgtaatttt gggaaagaac agcacaaatt caaagatgta aaagggaaac
     2221 caagtcgata taaaatgact ggtagttcca aagataaagg tcataaaggt atgtactgca
     2281 atgacaatcc tattggcaga ggtgtatcgc aaaactgtac agtaacccct ttatctaatt
     2341 ggtctgttgt tgaagaaatt aaacgcctta tagaaatcaa tagcatccaa gatgttgagc
     2401 cagattatca atttcataaa gaaggctgct cggaagaaga atcaacccca ttcaatgagc
     2461 cgaagcaatt ttacaaaagg gggaaaagta ggggcaaatc tcaacgctat caatgtaagg
     2521 cgtgtaagaa gttcactaat gttctaccaa aaagagaaga aaccactact tatcaccaac
     2581 aaaagaacac cattcttcca atgttcgcta agatggttgt tggaagggtt tcagtcagtc
     2641 gtacttgtga tattttagga attggagttg gaacctacta tcataagctg gagtggctgt
     2701 ataggcgttg tttggagttt ttagaacgat atgagacaca accccttcaa acaaagaaat
     2761 ttaacgagat gtggcttaat acggacaaga tgcactacta cctaaataac gtccgtaaga
     2821 aaggacaagg ttcaaagaaa tatacggggt ttgaagacct taatatgcaa acgtatatag
     2881 ttgtatctgc tgaggtctta tcaagatatg tgttccgttc tgatgttgcc tatgattgga
     2941 atatttcaat ggatgaatta aatgaagata caagaaaatt caaagaagac catctcaata
     3001 cttttagtcg taaaaatgat aggttagatt ggtcttatta tccacaagaa ccatccgcta
     3061 acgatagcga aaataggaat gcttatcttc acgaattagg gaaaattact aataggagta
     3121 gatttgtcga tggtttaaat gttgatgctc cttatactac aactgctcac tattggttaa
     3181 ttaaacaaat ggttaatgcc gacgaatggc gaatgattag tgatgatgac ttctctatta
     3241 gaaacgcctt ttatagagta ttcacgaaag aattaaggtt gagcgatgca catcatttta
     3301 tttgccaagt taacaagacg aaaagcagaa aacaatgctt aaaagaattc ggacaagcaa
     3361 aagcagaact gctcgattgg ggagatatca gaggattcaa aacaaagttt ctacggactt
     3421 tggcatctca ttatctaact gaattactaa caagtcatca attccacgaa gaggctatta
     3481 gtaaagatgg agaaagatat cgcaaatatg ccgataaccc tatcaaacat ccattagcca
     3541 ctaaggataa aggattttat tcagttgatt gtagaacgga tttatctgct ctggaaccaa
     3601 acgaaatcgc aaagatgcta ttaaatgtta atgaccattc gaccaatagc tttattcaac
     3661 agataagacg ttatatatca tctcttgaaa gaccattaac aacagctcgt ggtgataaga
     3721 agagctatat ttacgctaac ttcaatccga agtatgctca atttgcgatt acaattctaa
     3781 gaacttacta caacttttgt cgccctttta aatcagcgga taaaaaggtt ttgacacctg
     3841 ctcaaagatt agggattaca gataaacaat ttgattggaa agatataatt tatttcaaat
     3901 gacaaaaaga gcttggagta atcccaagct cttgtgcttt atctttactg ttcttcttca
     3961 attaacgcac ccgttaactc aataagtttt ttctttcata tacatgaaaa tagtagttat
     4021 acggattttt atcattcttt atcccttttt gggcaaatac ctcattccat tcctcataat
     4081 tcacttctgg aaaaaaagta tctccttcga attcatgatg tatttttgtg atgtacattt
     4141 tctcaacata agggaaaaac aaattataaa tctgttctcc tccgaaaata aaaatttctt
     4201 cttcgttttt acataactca aaaacatctt ctattgaatg aacaatttca caaccattaa
     4261 aggtaaaccc cttatctctc gtcagaataa tatttcttct gtcaggtaag gctcttccga
     4321 ttgattcaag gttcttccta cctaatatta tcggatgtcc ctttgtagta tttttaacat
     4381 attcccagtc cttgggaatc ctccaaggaa tgtcattctc tttgccaatc actctattct
     4441 tatccatcgc agcaatcaaa gaaactttca tttataacac agccttttta actaatttac
     4501 catctaattt cttatttcta tcaaaaaacc ttttctcatt atcctgcccg ttagttgaat
     4561 aagcaatttt ttctataagt tatattatca taataccaca aaatcacaaa ttttcattca
     4621 attatcattt tcaattgcat tttataaatc attttgtatg taatttggta tgtaagttaa
     4681 aatgaaaaaa gtagtccatt ttgaagtaca ttttgtcatt ttgtactcca aaactgaact
     4741 acttagtata gattactgtt gcttctttac taatttaggt ttacttgtta agaaagatta
     4801 tttacgcaaa tgttcttcaa caatttgctg agatttatca gctgtatctt ccacttcatc
     4861 ttttgctttt tctaactttt gaatatattg tttcaagctt gagttttcag cagttgcttc
     4921 gtctaatttc ttttgtgtat ctgttaactg agtttctaca tttgtttttt cttgtgtcag
     4981 tgttgcgatt tgttgattca aaccatttac agtattttgt aattctgaaa gttttgcttg
     5041 tgcttcttgt agtcttgctg tccaatctgc attcagatga ttgatttcat catttttttg
     5101 gttgatctca tctagtttga cattgatttc attttgtttg ctttgtagtt gtgcatcctt
     5161 gctgactaat tgactttcta agttttgttt ttctgctgcc gattgatttt tttggttttc
     5221 taattcattt acttttgttt ggtattcttg gacatctgcc tttgaatctt gcaatgtctg
     5281 ttctttttgt gctagtcgag cggtaagatc tgtaatcgct tgctctttat ctgtcaatgt
     5341 atgatcaagc tgtgtcagat tctcattgat tgtatcaata tctgtctctc caccccacaa
     5401 aataatttca tttgcgtacc gcgtgcctgt gtagccaatt agacatagaa atgcaacgac
     5461 aatcccacca gtaataaatt tctttttcat ttgcatagct ccttaatcca atacttttac
     5521 atgctaaaag taagtgatct caaacacaat gtcaagcgtt tttaaattac atatgtaatt
     5581 ttaatataaa tactatcaaa tactcattta atcctccttt tttatctatt taaaaaatct
     5641 tttttataga gaaaaatgca ggttaatact ttcttaaata aatatttatg gtgaacaaaa
     5701 acaaaaaagc aagttttatt aaacgtaacc cttttcattt catagattac tgtatttatt
     5761 acaaaaaacg
//
LOCUS       DACEUZ010000132         5766 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.132, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000132 DACEUZ010000000
VERSION     DACEUZ010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5766
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.132"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            221..655
                     /locus_tag="IXG44_002274"
     CDS             221..655
                     /locus_tag="IXG44_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ1658973.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            678..998
                     /locus_tag="IXG44_002275"
     CDS             678..998
                     /locus_tag="IXG44_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1658974.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            1139..1330
                     /locus_tag="IXG44_002276"
     CDS             1139..1330
                     /locus_tag="IXG44_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1658975.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(1401..1658)
                     /locus_tag="IXG44_002277"
     CDS             complement(1401..1658)
                     /locus_tag="IXG44_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HAQ1658976.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            1816..2079
                     /locus_tag="IXG44_002278"
     CDS             1816..2079
                     /locus_tag="IXG44_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1658977.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            2103..2291
                     /locus_tag="IXG44_002279"
     CDS             2103..2291
                     /locus_tag="IXG44_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1658978.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(2374..2559)
                     /locus_tag="IXG44_002280"
     CDS             complement(2374..2559)
                     /locus_tag="IXG44_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1658979.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            2774..3145
                     /locus_tag="IXG44_002281"
     CDS             2774..3145
                     /locus_tag="IXG44_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ1658980.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(3295..3367)
                     /locus_tag="IXG44_002282"
     tRNA            complement(3295..3367)
                     /locus_tag="IXG44_002282"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3332..3334),aa:Gln,seq:ctg)
     gene            complement(3413..3841)
                     /locus_tag="IXG44_002283"
     CDS             complement(3413..3841)
                     /locus_tag="IXG44_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1658981.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(3901..4659)
                     /locus_tag="IXG44_002284"
     CDS             complement(3901..4659)
                     /locus_tag="IXG44_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HAQ1658982.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            4954..5151
                     /locus_tag="IXG44_002285"
     CDS             4954..5151
                     /locus_tag="IXG44_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1658983.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            5257..5739
                     /locus_tag="IXG44_002286"
     CDS             5257..5739
                     /locus_tag="IXG44_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1658984.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
BASE COUNT         1997 a          873 c         1142 g         1754 t
ORIGIN      
        1 cgttttttgt aacagaatga tgttcgctcg atgtgaaaaa tgatgaggaa tttcacataa
       61 tgcagaaata atgggattag aacaggtgtt ctgatgagaa aagaaatgta taaataactt
      121 gaaagaaatt gcttttgttc atcattttat ttacatattg cgtatatttt ttgtattttt
      181 gcttgagatg ttacaataaa agaaaaagga ggcggttcca atgagtaaaa gataccagaa
      241 catcatggta gcagttgacg gatcaagaca atcgatcaaa gcgttcgctg aagcccttga
      301 cttagcaaag gacaatgaag cccacttatt tatcgtgtca atcatcaaca aagttgagct
      361 gacccatagt gcctatgcct tttcaaaaat ctatgcagat gaaaaacaaa gaacagaagt
      421 agccatgctg aaaaaaatca atgatgccaa agagtacggc attgctgaga tccacgcaat
      481 agttgaaact ggagatccaa gaaatttgat tgccaatgtc atccctggac aagaaaatat
      541 tgatttgatc gttatgggag cgacaggaaa aggtgctata caacaagcat tagttggatc
      601 gaccgcttct tatgtcgtat cacatgcccc ttgcagtgtg ttggtcgtga agtaatgaga
      661 taagggagga atagaaaatg gttcgtcctc aagaagtccg tgcgccaaaa gaaaaaatag
      721 aaattttagc aattattgaa gacggcacac aaacaaagaa gggatactcg atcgctttag
      781 taaagtggaa aggaaaaaaa ggtgttgctc tgagatggga tggagataac caacaagata
      841 aaggattccc tgtcacagct aacggttatc atccagcgtg gtttgttctg cctgataaat
      901 ttatggaatt atacagctat gattatgcaa gagccgcagc atcaatcaaa gcattagata
      961 aattaacaga agagcagaat gaaatggagg aaaaataaaa aagacgaacc aatcagcctt
     1021 tagaaaactg attagttcga ggagtaaaaa tgaaaaaagg ttatttggga tgaaactaga
     1081 ataatgcgta taacttaaat aaaacttaaa gcaataaaat ggcaagaggg tgaggcaaat
     1141 ggaagcagga aaagtattac aggaagtaaa agcactatgt gcagataaaa aatatgaaga
     1201 agcgaaaagc ctgatcgaaa agaacaaaga aaatttggaa gaaaagtacc cgatcgcaca
     1261 agaatttata gaattaaaac aaggaacgat ccttcaccga ttcaaacatt tttttggtat
     1321 aacagaatag aaaaaacgat taaagatcgg cagaaatttg ctgcatcttt aatcgttttc
     1381 tttttaaaca tgcgagggat tcaattctga ttcttatata aaaaatagat ccaaataata
     1441 agaaaaatta ctgaaagcgt caattcgatg atgtgtgtta ttaatacaga cgttgtttcc
     1501 caatgcctaa aagaaaaaat ttgataagta aaatatacag caggtaaaga ggccagtaaa
     1561 actgaaaact tcttttttgt ccattttatg taatagaaaa gaatgcttat cataataaaa
     1621 aaaggaatat aaaaaacaaa ttgaccgatc gcgatcatac tattgcctcc gataaataaa
     1681 gtccatgata caaaataaca ctttgtgcga aatggcgcaa gcagttttgt ggatttatcc
     1741 ggtaaaatct gtatcgattt tgaaattaga ggagaaactt gctataatca aagtgaaaga
     1801 aggagtggga ttttcatgga tcatgtagtg ttagaaatcg atttgcattt gatcaataat
     1861 attcgggtga tttactatgt tgtaagtaac agtgtcgaac aacgtgtact gacgaataaa
     1921 ataaatggta ttttagcaaa gaaagacgtt caccgattca ataatggtga aggaagttat
     1981 tatagtatcc cagtagaaaa aattatttat acgacagtca aagtcagaga agatctagat
     2041 accaaaaaag catatgaacc tatttttaca acttattgat tgacagcaga tggaggaaaa
     2101 aaatgaatcc atatgaacaa cttattcaag atttgatcga tggaaaaatc gaaaagattg
     2161 atgtaaaaag agaagaatta atggcttttc gagaagcgtg gctgaagttg gagaatcgta
     2221 aatatgtggt gggaaaagcc tcactgaacg gcaatgtgac ttatcattac gatccgacac
     2281 gtttatttta gtatgaagta ttcagaaaag aaccaaagga ggacgatgca gcagcattgt
     2341 cctcctttga ttttacaaaa gtgaaagttt tacttatttg aatagatttt taaatttgtt
     2401 tttgacactg tcgatattta catcttttaa aagattttgg gctttctctt tataaggtcc
     2461 taattgttct ttatgttctt cgataaatcg ttttgcgcca tcgaaattgc cgttcttaat
     2521 catttccttg actttttgaa ataattcttc ttgattcatt ttgaacacat cctttgctat
     2581 caacatgatt ttttacatgg acaaaacgag aaaaactgat ttatccacac gtcaataata
     2641 tcaaataacg atcattttta ctaaaaaaac actcgattct cgcttctaaa gtaggagaaa
     2701 aatctccaac ttgggacgga tggaatttct ttaactgacc catatactga atatataaca
     2761 aggaggtaga aagatgaaag tgattaaata tttgattgga ctggcggcga ttagtggtat
     2821 tttgctattt ggactgaaat attatactga aggaagttat ggagaaatcc ctggtatact
     2881 tgatcagcta aatccattag ttgaaaaagg agaggtctat gtaaagacac aaaaaccaga
     2941 ggaagtaaat gaatatggta cagcaaggta tgtgcagaaa gctgctgatg ccaatgggaa
     3001 agaacgaacg gttgaattta atggtctttc tgtgttaaaa gaaaatcatt atttgaaaat
     3061 tagcaataaa ggagcacaca tagaaacgta tgaggaagtt cctagagaaa aagttccgga
     3121 gaaagcatta gcaattattg ggtaaatcag tttttgtatt aagaatacgc ccgattttat
     3181 tattcataat ttaattatgt aaactaaata caagtagtaa aaataataca ataatccccc
     3241 tgcaaaaaga ttgttctttt acagggggtt cattttttca gcaagcacat caaattggga
     3301 tggcagggtt cgaacctaca attgcttgat tcagagtcaa gtgccttacc agtttggcta
     3361 catcccattt attcaacaaa ataatcatag cactttccta aaaaaacgca agttattttt
     3421 cttgatcgga tagttgttgc tttactcgta aaaggcggcg tactagtttc ttttctaatc
     3481 cagcaggaag ggcaatcagt tctgctactg acaagacggt gtccaataac tgatattctt
     3541 tgacaggttc attcataata tgatagatgt ttgctaaacg ttcaatatat tcaaagcgag
     3601 tatactctgt tcgagcaatt agaaatttca ataaatcggt ggctttctcg tactcttgat
     3661 ctgcgtaata aacaagagat tgacgataca atttgatttc cagttcattg agggtagcag
     3721 gtctgcgacg ctcgttccac tcattaatag agactttacc ttgaacattt cccttgcagt
     3781 cacggacaaa aataatcgga atttcatttg gatccatttt tttatgtgtg aatttttcca
     3841 ttatctacgc tcccttcaaa ggtttggtat cttgagtata agggattttt gtttgaatta
     3901 ctagcttttc tccaaagtta gcatctgtcc acccattgca gtgaaaatcg ccgaaaaatc
     3961 gttgatcggt actgctcgat tttttacacc atagggatcg ttgacataaa tattttcttt
     4021 gtcatatcca gtgactacac aagcatgaac aagaggagtg actcttactt ctccattatt
     4081 tgtggtccaa gtcataaaat cagtttcatc tgggacttca aaattgatag tcacagaagt
     4141 ccagacagga tttcctttag atagtgcttt tttgatatca tcgagttgag catcggtttt
     4201 tggaaaagtg tgataatccg tttggacgat ttgctgagca agggaggcaa ctgggggaac
     4261 atagacgacc atagcccagt ctccgccact gatatcacca acaaatcctt cattaggatc
     4321 gccatgagtc ccatctgcat tgaaaacagg gacgtaatcc aattgctcag ctaactggtt
     4381 tttagttgtt tcataaccat aaaaagataa aagcatagaa agcgaagtga cttcacagcc
     4441 attttctaaa gaaggagaat caaactggct ctctaatgga acatctagta aaacttctgc
     4501 tttctctttt gtctttttcg ttttttgtgt tgaacttttt tgtgcagtgt tcattgtatg
     4561 ttctggagaa tggatatgta ggtaataact tgtaccgctt aataaaagag cagctgtgcc
     4621 ccctagaaac aatagttttt tgaacgtttt tttcttcatt tgaaatctcc cttttcagct
     4681 gtttatcttt cctattataa atgtattttt attggaaatg gaagtattat attttaatat
     4741 gtaggaaata attagttttt aaggaatatg ttttaatttt ttgaaaaaaa ttcttctaaa
     4801 tacaacagct gttttaattg tgaaaacgaa aacagtacgc tataatgcct gtaaggttga
     4861 tttttaactg taaataaaaa tcaatgagtt aaatctccaa agaaatagca gtggtaatgt
     4921 ggagaaaagt tccagcagtt taggaggatt ttgatgacgg aagaaatgat taatttagga
     4981 gaacaatatg catgcaaacc aattggtttt acaaaaacgg tcattggtga agttgtatca
     5041 aaaatgacaa actgcgccgt ggtcaaagtt gcccaatgtg ctgcagagga ccaagaatta
     5101 ttagatgaaa aagcaagcat ggttgttgca aaatatgata catttgaata aagaaaacaa
     5161 ttggacgtgc ggcgtgctgc aagtccaatt tctttgagaa ggtaaatcag tattcttttc
     5221 ctattttctg gagaagaaaa agcgaggcga gttgtcatgg taacattctt tacggttcta
     5281 ttgtttttat tcgttgttgt gatgttttta tttctttggc tggttcgaaa agaaattatt
     5341 tatcggactg tgcgaaatcg atgggtctac ttggtgatac ccttcttagt gtcactagtc
     5401 atttggtata cactcatcag tcatcctaca gcagatgaat tggcaaaagg gatcttgtca
     5461 gcaatgattt ttattagttt tttgttggac agtagaggaa tcacagaaga aggacttgtt
     5521 ttgaatagct ttgacaaaaa aggtgttcct ttttctgaga tcaataaaat tgtattatat
     5581 cagccaaaag gttctaaaat cgtaaaaatg aattttttcc gaaacggatg gcgaggaccg
     5641 atgctgaaat tttctgcatc attagaagaa ttagttccat ttttgtccca acgcttaaac
     5701 gatgaggcag agattgatat tatgattgat ccagaataaa aacagaggtt gcgacaagat
     5761 gtttgt
//
LOCUS       DACEUZ010000133         5686 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.133, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000133 DACEUZ010000000
VERSION     DACEUZ010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5686
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.133"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(129..800)
                     /locus_tag="IXG44_002287"
     CDS             complement(129..800)
                     /locus_tag="IXG44_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1658985.1"
                     /translation="MIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGITFIVES
                     LLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWDKILHA
                     VSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLCDQFLG
                     MNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFESYKLE
                     KVKNT"
     gene            complement(793..1287)
                     /locus_tag="IXG44_002288"
     CDS             complement(793..1287)
                     /locus_tag="IXG44_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAQ1658986.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(1307..2527)
                     /locus_tag="IXG44_002289"
     CDS             complement(1307..2527)
                     /locus_tag="IXG44_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1658987.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            2674..4509
                     /gene="lepA"
                     /locus_tag="IXG44_002290"
     CDS             2674..4509
                     /gene="lepA"
                     /locus_tag="IXG44_002290"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HAQ1658988.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
     gene            complement(4603..5685)
                     /locus_tag="IXG44_002291"
     CDS             complement(4603..5685)
                     /locus_tag="IXG44_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017414133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1658989.1"
                     /translation="MNDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTDT
                     RVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINAE
                     FKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKIIS
                     LEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVKN
                     DGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKEDG
                     ELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISIT
                     MDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT         1738 a         1143 c         1084 g         1721 t
ORIGIN      
        1 aaaaaagggg tctgacacaa ctagtgtcag acctcctttt ttcaaataaa cagtgttcca
       61 gaagtagttt cttcgaaaaa taagccgaaa tttcttgaag ctgcccgctt ctgtcacaac
      121 ctcttttatt aggtattttt cactttttct aatttataac tttcaaagta acgctcatct
      181 tttttacttt gagaccatgc aaacaatccc attaaggcag cacctatcgt atttgtcagc
      241 aagtcgccca tcgtatccat caatgctgct cgtccgacaa acagtgtgcc atcagaagca
      301 gcaaaacgtt gaagattcat gcctaagaac tggtcgcata agaattccca gaactcccaa
      361 aacacgccgc aaagtccagc aaacgcaaag ccgaataaga ggaacaacca aggactggtt
      421 tttgatatct cagcatcttt catcaagtaa ccaatcaatc catagccaag agctgtcagg
      481 accattggac tcaccgcatg taagattttg tcccaaaaag agatgatact gatcaaatgc
      541 atccctgtgc ctaaaaagac agagatgaac aagaaaaacc agtaaaacaa aacgattgct
      601 gctggtattt taagtttgaa aactttcaaa atgacttgtg gcatgaaaat caatgcaatc
      661 cctaataaag attccacgat aaatgtgatt ccttgagaag tcgaatattt ttgtgtgata
      721 aattcataaa aattatagca gacaacgaat acaccaaaag caagtaaagc tatttgcacc
      781 tttttgattt ctttaatcac tgaaagtccc tccatctaaa aatcttttta cttctgaaag
      841 caactcaatt tctgaagaaa ccacttttcc gttcaatttg atcaaaccta ttgtatataa
      901 gttcaggtag tgaaattgat tttcagctgt ttcttgtagt gcagcgattt ttttcgggtg
      961 ggttccgcct tgctgtcgag tatcagtata taagccgatc actgggatgc ctttcgcata
     1021 agccacaccg atttccgatg ctaccccggc atcgatcgtc agcccatcta gtaaagcaat
     1081 cattaaatca ctagccaaca ctttttctgt gtcagcaaga gcaatcattc gactgtctgc
     1141 ataagcagtt ttgtcattga ttgcttcatt ttcttgagga agatagatcg aaagctcaga
     1201 agagagctct ctgatttctt taactaactg ttggttatat aacaaatcac ttttagcaaa
     1261 cattggtgcg gcaaaataaa ttttcatcta aacatcatcc tttttatcaa aaataatcgc
     1321 taaagtaagc ttccatgttt ggaatgatac gctctagtga cttcagtgtt tcttcatctg
     1381 tttctaaccg ttcgattctt gccaaataag aagcacggcg ataattcatc atcaagcaag
     1441 tcaatgtttg gatatccagt cgaacggatt ttcctatagg ttcatttaag acacggactt
     1501 gtccctgttc atcccatgtt aaagcgaaga caccattatt ccaaggagcg acaggatctt
     1561 caatgatgaa atgaaaagca tcagctgttc caacaaacgg aaaccgttga aggaattctt
     1621 ttacatcaac gatccgagcc ataaaatatg gttcgatctg ctctttgatc tgactatctt
     1681 caatcaaaaa tgctaaaggc tcatttttat aaatatctcc atgtacccag tagatcatgg
     1741 aaaaatgtgc ggaaataaag ttccataagc catttcgagc ttcttgatca agatagaaca
     1801 tttcttttac gtgaaatatt tcttcagcta cccagtagaa cagaacacct cttggttcat
     1861 ggttgctatc gtagtagaca gcggctgtgc gctcatcttc attttcgaaa cgccaatact
     1921 cttcccaatg aaaatcactg cggaataaag caccatggtt ttgttgggca aatttgtcat
     1981 aaacggaaaa tacgtctgga tgatcaactg gtaatcgctc aaccatacca cttactggga
     2041 tttgtttagg tagctgtgta tctctgattt taaaggaaag tttatcagac atgatttccc
     2101 atcctttacg ccgatagtag gggatactgt aaggatacaa ataagagatc cattgcttat
     2161 tcttgcgcat attatccaat gcaacgataa ttagatcttg catcaatcca tggttagcat
     2221 attctggata agttccaact ccagtcacac cacccatttt ataacgtgca ccgtgaatat
     2281 tgacttcaca aggatagata gcgatttgag agatcagttt attttcgtgg aaccatccga
     2341 acactttcga tacttccaaa atcggttttt tggaacggat aaaagctcgt ttattctcaa
     2401 acccgctttc ctcgatgtct gcttcagtca cttggaacac ataggaaagc aattcattga
     2461 attgatcgat atgttcttga gtgaccggtt ttaattcaag attttctcta aaatcttttt
     2521 gatccatttt ttcatctcca tttattagct tgataacagt atagcaaaat tctacaaaaa
     2581 aaacttctat agctgactaa ttaaaaactc tttaaattac tgcttttcgt tggtataatg
     2641 gaaagtgctg attatatgaa aagaggagac gctatggata taaagaaaat gaaagaacgc
     2701 caagagcaga ttcgtaattt ttcgatcatc gctcatatcg accatgggaa atctacattg
     2761 gccgatcgta ttttagaaat gacgcacact gtgacgtctc gcgaaatgca agatcagctg
     2821 ttagactcga tggatttgga acgtgaacgt ggaatcacga ttaaattaaa tgctgtagaa
     2881 ctcaattaca cagcgaaaaa tggagaaaca tatatctttc acttgattga caccccaggg
     2941 cacgtcgact tcacctatga agtctctcga agccttgctg cctgtgaggg tgccgtttta
     3001 gtggtcgatg cagcacaagg aatcgaagca caaacattgg ccaatgttta tttagcgtta
     3061 gacaatgatt tggagatcct gccagtcatc aataaaatcg acctacccgc agcagatccc
     3121 gaacgtgtcc gtcaagaaat cgaagacgta atcggaatcg atgctagtga agctgtatta
     3181 gccagtgcca aatcagggat tgggattgaa gatattctcg agcagatcgt ggaatatgtt
     3241 cctgcaccaa gtggagactt agatgcaccg ctaaaagctt tgatctttga ctctatttat
     3301 gatagttacc gcggtgttgt tctaaatgtc cggatcacgg acggtatggt caaacccgga
     3361 gacaaaatca aactgatgag taatggcaaa acatttgatg ttacagaggt cggggtcttc
     3421 tcgccaaaag cagttgctag agactttttg atggtcggtg atgtcggtta tatcacagca
     3481 agtatcaaaa ctgtccaaga tacacgagta ggggatacag taacgttagc agacaatcct
     3541 gccgctgaag ctttaccagg ttaccgaaaa atgaatccga tggtttattg tggtctttat
     3601 ccaattgata cttctcgtta caacgatttg cgggaagctt tagaaaaact gcaattgaat
     3661 gatgctgctt tacaattcga accagaaacc tctcaagcat taggttttgg gttccgctgt
     3721 ggattcctcg gacttcttca tatggatgtc gtccaagaac gcttggaacg ggaattcaat
     3781 ctagaattga tcacgactgc accatccgtt atttaccatg ttaataaaac ggatggtacg
     3841 actgctacag tagataaccc ggcagatttt cctgaacctg taacgattca ggatgtagaa
     3901 gaaccattcg tcaaagcaca gatcatggtc ccaaatgatt ttgtcggtgc agtaatggaa
     3961 ctttctcaac gaaaacgggg agaattcatt acgatggatt atttagatga ttaccgagta
     4021 aatgtcgtct ataatattcc gctttctgaa attgtctttg actttttcga caaattgaaa
     4081 tcaagcacga aaggctatgc ctcattggat tatgaaatgt ctggttatca aaaaagcaag
     4141 ttagtgaaga tggacatctt gctgaatgga gaaaaagtcg atgcattaag ctttatcgtt
     4201 caccgagact ttgcctatga acgtggaaaa gcaatcgttg aaaaactgaa aaaactaatc
     4261 ccacgccaac aatttgaggt gccaatccaa gcagctattg gacaaaaaat cgttgcccgt
     4321 tcagacatca aagccttgcg taaaaacgtc ttggctaaat gttatggtgg agatgtttct
     4381 cgtaaacgta aactcttaga gaaacaaaaa gaagggaaga agcggatgaa acaaatcggc
     4441 tccgtcgaag ttccgcaaga agccttcatg gccgttctga aaatggacga ggacgaaccg
     4501 aagaaatagc aagggtttgt ttgaaaacag tgtaaaaaat aaaccatgtt tacatggatt
     4561 tgccaccggt ttgccaccgg tggttttttt cggtggcaaa aatcaagaat tctttctaag
     4621 attttccatg atatcgaccg tttcatttct catcttatct gttacatgcg agtaagtatc
     4681 catcgtaatt gaaattctgc tgtggcccaa tcgttcagag atttctttca tttttgcgcc
     4741 attttcgaga agaagtgtag catgagtatg tctgagagaa tggaagttaa aagagaggga
     4801 gagtgcattc gatatcctcc tagtattcca tttcactaca cttggcgtaa ctagctcacc
     4861 gtcttccttc gtacatactg catttgaatc aatgtagagc tttccatact tcattcgatt
     4921 ttctaattgt tgtttcttat gttttttcag aattgctagc aaggtttgtc caataaaaat
     4981 cgttcgattg gaactgcttg tctttggcgt accatatacc catgcgccgt cattctttac
     5041 catttgtttc tctacagtaa ttgttccatt tgaaaaatcg acatcatccc acgtcagacc
     5101 acaaacttcg ccaacgcgca ttcccgtata aaatccaata ttcaaaggaa tatagaaagg
     5161 atgtccttca ggagtaattt ctagcatatg atcaaagtcc tcaagagaaa tgatttttag
     5221 atctttttta gtcgttggtc gtgcttcata ttttggtatt tttacataca gcataggatt
     5281 ttgcttgatt aatccccaag gataaaccgc catattcagc gcattcttaa ggacagagtg
     5341 agtaatagtc attgttttct tcgagtaacc ctttttaaat tcagcattga tgaaattttg
     5401 taaaagagca ggggagagat ccgtaagttt tttctttcct aaataaccgt ttatatgatt
     5461 tttgatggta aatcggtagt tttcataggt attgtatttt agatttagtt taacgtattc
     5521 ctccatccaa aaatcaaggt attgttttac tcgagtatcc gtacctaaaa agtattgtcc
     5581 tgtttcgtca atatctgata aaacttttcg taaagcagct tcggcctctg gtcgggtgtc
     5641 tccgccaact ttctccactt tttttcttga gccatcatca ttgata
//
LOCUS       DACEUZ010000134         5673 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.134, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000134 DACEUZ010000000
VERSION     DACEUZ010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5673
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.134"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            339..956
                     /locus_tag="IXG44_002292"
     CDS             339..956
                     /locus_tag="IXG44_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1658990.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            997..1296
                     /locus_tag="IXG44_002293"
     CDS             997..1296
                     /locus_tag="IXG44_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1658991.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            1330..1497
                     /locus_tag="IXG44_002294"
     CDS             1330..1497
                     /locus_tag="IXG44_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ1658992.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(1542..2147)
                     /locus_tag="IXG44_002295"
     CDS             complement(1542..2147)
                     /locus_tag="IXG44_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1658993.1"
                     /translation="MGKIGYIRVSSQDQNLDRQLAMMEGLNIDKLFQEKASGKDTERP
                     EFQKLLNYIREGDCVIVTSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQ
                     KRLVFKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
     gene            2272..5259
                     /locus_tag="IXG44_002296"
     CDS             2272..5259
                     /locus_tag="IXG44_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HAQ1658994.1"
                     /translation="MDEIKSNKKAFGIYLGEPSTEQLNQFFYLHEKDLELMETIRRPA
                     TRLGFALQLVSLRFLGTFILDLTNIPQNVLSYVATQLNIDPGEIIYYSRRQTRYQHTK
                     LIKSHYGYQDFNQPEIETYLANWLLNRALYTTESSDMLFDMLLKKCLDEKIVLPGITT
                     FSRFVASAIEKAEEHLYRQLSLVPTEDERKRLLNHLELIGTPVYGAIIKMDILRTPLA
                     DDSHKEIARGFDRLKQFQAFSTEKWKMESIPEGKIKVLANYAFKAKAQLIQRMSEQKK
                     LALLVAFIFIYKRKAMDEQILALSNFFETVFRRAKNKESKERLRTIKDLDRAASTLSD
                     IVDLVMSSHSDTNELSLRDRILEKYSEENVTEAVSQVRLLVKNDREPIAISELLNTYR
                     KFRKFIPDILTTLTFDSNDYGNNCKELWTFINNRFPKPITYKMFGAIKHTLPKKWIYY
                     IHEYPEQTNKCVLIAAVELLIQSLKRHDIYIPQSVLYNNPMDCLIDEKTWVQQKYVLL
                     EQLDLPSSANEMSLWLKNDLELSYKEALNRWPTSKMARIENADRLVVSRLKKTYEKKE
                     DKSFRRRIQQLLPKIDLSDLLLEVNQQLNLTQSFNHLSDKDTKMKDLDISLLAVLLSE
                     SCNIGFSPVSKEGFDSLKYDRLVYVNHHYIRVDTLSGANQKIIHAHRKLATSKIWGDG
                     HMASADGIRYITPQRSLYSRSNPKYFGRGRGITFYNFVSDQYIGFHGMVVSGTLRDSL
                     YLLEGFLNQPSELTPNQIMTDTAGYSDMIFGLFGLLGFQFSPRIANDKGTKLWRIDAN
                     AHYGELDTLSQNSINMELIYTHWEDILRVAGSLKSGKVNATELTKSLQRNGQPTPLGK
                     AITEYGKVYKTKHQLRYISDEIYARQILEQLNKGEARHALCRNIFYGRKGKLYQTYYD
                     GMEEQLNALGLVTNAIIYWNSLYLEKVFEQMKAEGFDCSEEMIRRQSPLISDHINFVG
                     KYTFRYEAELSNGKLRPLNVEEF"
     gene            5408..5617
                     /locus_tag="IXG44_002297"
                     /pseudo
     CDS             5408..5617
                     /locus_tag="IXG44_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346508.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1912 a          952 c         1036 g         1773 t
ORIGIN      
        1 ttgtaataga ataatagtgt taaaaaaatt gtaaaagtta aagaagtcaa attctgatgt
       61 gaaaaataaa atataccatg gaagacataa aagaaaaact ggataactag tttatttaat
      121 ggcagaccgt taaggcctgc catttttatg tattttagcg gaataaatat aatcatgcgg
      181 gagtgtaaac ttactgtttc aaaaagtata ctttttgaca tgccttgatt ttctttgttt
      241 atgtatggca taataaccac ataatacaat ttgaaacgtt tctgatttaa tctgattgtt
      301 ttgaaacaat tttagtgaaa aaagggtaga taaaaacgat gagaaaattt ttttatatcc
      361 gggtcagtag ttatgatcaa tcgatcgaac gacaactgat ttccgcgaag gaattaggaa
      421 tccctgaaga atatatattt gtagaaaaag ccagcggcaa agattttaaa cgccctgaat
      481 atcaattaat gaaacggatg tttcgaaaag gagatttact ctatctacaa tcattagata
      541 gactaggcag aaataaacaa atgattttag aagaatggcg agaattaact aaggaaaagc
      601 agattgatat ttctgtgtta gacatgcctc tgttagatac tgctagttac aagcaactag
      661 atgggttaga gacgcttatc tctgacttaa ttctgcaatt actcagctat atggccgaag
      721 acgagcggaa acgaattaat agccggcaaa gagaaggcgt agaagctgcg aaacaaaagg
      781 gtgtaaaatt cggcagaaag aaaattgctt tacctcctga atttcctgcg atctattctg
      841 actggaaagc aggaaagata actgcagtct atgctatgga tcaattgggc atgaaacgaa
      901 atacctttta tcgacgggtg aaagaatatg aacatacctt gcaccacttt tcttgataga
      961 tataacagct attagacggg aagggagagt ttgaaaatgg acttaacgaa tcaaagggtc
     1021 gtttttactg gaacgctgca gcaaatgaca cgaacccaag ccatcggttt ggttcactct
     1081 ttaaacggcc attgtcaatc aagtgttact tcaaaaacaa atttcctggt ttgtggtcag
     1141 tattctaagt cgttgtttga tgattctctc tatacaaaaa aggaacaaac cgcgcgggac
     1201 ttgattgcgt taggtcatga gattaccatt ttatcagaag tcgaatttta tgcgttaatt
     1261 agtcaacagt tgaaagaatg gcaacggtat ttataatcgt tagtaaaatt tacaaggagg
     1321 aaaattttta tgaagcgaaa atcttatgat cgttggggca ttgaaataac tagttttaat
     1381 cgaaatattg cttttcacgt ggagtgtgac tgtggggaat tagcaaaaag tgtttatcaa
     1441 aaaaattact tttactgtcc taaatgccat cgccgatacg gggtacatgg aacatagaaa
     1501 cgaaatagtg cggtagaaaa gcgaatgaat gtagtatatt tctacaaatc attcgctttc
     1561 tttattttat aaatagttgg tcgagagatc ccgttttctt ttgcaatctc tgcaactgga
     1621 atgccttgct ttaacatgtc caccacggtt ttaaaaacta agcgtttttg cggatctgtt
     1681 gagttaatag aatactcgat tggtcgtccc ttatatcggc cggctttttg agccaataat
     1741 actccttgcc gctgacgttc acgaattttc tcacgctcgt tttgtgcaat ataacttagc
     1801 agtgataaaa acatatcgaa catagcggta tccaggagtt catttccagt attaaaatct
     1861 aaaaactgtg catctaatat ttttaacgct acttttttct gtttcataaa agcaactgta
     1921 ttttttatat cttcataatc tcttcctaaa cgatctagtg atgtaacgat gacgcaatca
     1981 ccttcacgga tataatttag taatttttga aactcaggac gctcagtatc tttaccactg
     2041 gctttttctt ggaataattt atcaatgtta agtccttcca tcattgccaa ttgtcgatcc
     2101 aaattttgat cttgtgaaga tactcgaata tagccaattt ttcccataaa aaactccttt
     2161 ttaaaagtga tataatttac atgtaaaatt tacttgtagt tttactgcta tcatagcatt
     2221 tttaaaaata aattttatgt agacttaaaa tttacttcat ggagggttta aatggatgaa
     2281 atcaaaagta acaaaaaagc atttggcatt tatttaggag aacctagtac agaacaatta
     2341 aaccaatttt tttatttgca tgaaaaagat ttagaattga tggagactat acgacgacca
     2401 gctactagat taggttttgc actacagtta gtctcactac gttttttagg aacttttata
     2461 ttagatctta ccaatattcc ccaaaatgta ttgagttatg tagcaactca actaaatatt
     2521 gatcctggag aaattatcta ctatagcagg agacagacca ggtatcagca cacaaaattg
     2581 ataaaatcac attatggata tcaagatttc aaccaaccgg aaatagaaac atacctagct
     2641 aactggcttt taaatagagc gctgtataca acagaatctt cagatatgtt gtttgatatg
     2701 cttctaaaaa aatgtctgga tgaaaagatt gttttacctg gaattactac tttttctcgt
     2761 tttgttgcta gtgcaattga aaaagctgaa gagcatttat accgacaatt gtctttggtt
     2821 ccaactgaag atgagagaaa gcggcttctc aatcatttag aattaattgg aactccagtt
     2881 tatggggcaa taatcaaaat ggacattttg agaaccccgt tagctgacga tagtcataaa
     2941 gaaatagcca gaggttttga ccgactaaag caatttcaag ctttctctac tgaaaaatgg
     3001 aagatggaat ctattcctga agggaaaata aaagttcttg caaattatgc atttaaagct
     3061 aaagctcaat taattcaacg aatgagtgaa cagaaaaaac tggcgttgtt agtggcattt
     3121 attttcatat acaaacgtaa ggctatggat gaacaaattt tagcactatc taattttttt
     3181 gaaactgttt ttagacgcgc caaaaataag gaatctaaag aacgtcttcg taccataaaa
     3241 gatctagatc gagccgcatc gacactttca gatattgtag atttagtaat gtccagtcat
     3301 tctgatacta atgaactatc ccttcgcgat cgtatcttag aaaaatattc tgaggaaaat
     3361 gttaccgaag ctgtatcgca agttcggcta ttggttaaaa atgatcgtga accaatagcc
     3421 atttctgaac ttctgaacac ttatcgaaag ttcagaaaat ttattccgga tattttaact
     3481 acactaacat ttgacagcaa tgactacggt aacaactgta aggaactctg gaccttcatt
     3541 aataatagat ttcctaagcc gattacttac aaaatgtttg gagctattaa acatactctt
     3601 ccaaagaaat ggatttatta cattcatgaa taccctgaac aaactaataa atgtgtattg
     3661 attgcagctg tcgaattgtt aattcaatct ttaaaacgcc atgatattta tattcctcag
     3721 agtgtcttat ataacaatcc tatggattgc ttgattgatg agaaaacttg ggttcaacaa
     3781 aaatacgttt tattagaaca actagatctt ccttcttcag caaatgaaat gtctttatgg
     3841 ttaaagaacg atttggaact gtcttataaa gaagcgctaa atcgttggcc tacttctaaa
     3901 atggccagaa tagaaaatgc agatcgcctg gttgtctcta gacttaaaaa gacatatgaa
     3961 aaaaaggaag ataagtcttt tagacgtcgc attcagcagt tacttcctaa aattgatttg
     4021 tcagatttgt tattggaagt aaaccaacag ttaaatctta ctcaatcatt taaccactta
     4081 agtgacaagg acaccaaaat gaaagacttg gatattagtt tattagcagt tcttttatca
     4141 gagtcctgca atattggttt ttccccggtt tcaaaagaag gatttgattc tttaaagtat
     4201 gatcgtcttg tctatgtcaa tcatcattat attcgagtgg atacactttc aggagctaat
     4261 caaaaaataa tacatgcaca caggaaactt gccacctcta agatatgggg tgatgggcat
     4321 atggcttcag ctgacggtat tcgttatatc acaccacaac gttccctata ttctagaagt
     4381 aatccaaaat attttggccg tggtcgtggg atcacattct acaattttgt ctctgatcaa
     4441 tatatagggt tccacggaat ggtcgtttca gggacactgc gagattcgct ttaccttcta
     4501 gaaggattcc tcaatcaacc cagcgaatta actcctaatc agatcatgac tgatacagct
     4561 ggttatagcg atatgatttt tggtctcttt ggcttattag ggtttcaatt tagtccaaga
     4621 attgccaatg ataaaggaac caaactttgg cgaatcgacg ctaacgctca ctacggggaa
     4681 cttgatacgt tgtctcaaaa ttcaattaat atggaactga tttataccca ttgggaggac
     4741 attcttaggg ttgcaggttc gttgaaatct ggaaaagtga atgcaacaga attaacgaaa
     4801 tctcttcaac ggaatgggca acccacgcct ttaggaaaag caataactga gtatggaaaa
     4861 gtttataaga cgaaacatca gcttcgttat atttctgacg aaatatatgc acggcaaatt
     4921 ttagaacaat tgaataaggg ggaagctcgg catgccttat gccgtaatat tttttatgga
     4981 cgaaaaggaa aactctacca gacttattat gatgggatgg aagagcagct caatgcatta
     5041 ggattagtta ctaatgccat catatattgg aactcactgt atttagaaaa agtatttgaa
     5101 caaatgaaag ctgaaggatt cgactgttcc gaagaaatga tacgcaggca atctccacta
     5161 attagtgatc acatcaattt tgttggtaag tatacgttta gatacgaagc tgaattatcc
     5221 aacggtaagt tacgtccatt gaatgtagaa gaattctagc aatgttcggg ttttgcatgc
     5281 taccgcacta tttcgtatga ctgttccagc gaatcacttc gaatagaaaa agagaaaaac
     5341 acgagaacag cgttaaatca acgtttcttg tgtttttact tttgtgctac cgcactattt
     5401 cgtttctatg ttccatgtac ccctttatca agaagcgcaa acacgccaaa ttgccgattg
     5461 gttgatcggc atgaacgcaa gccctttata taccttgaat ttacaacaaa aaggcgtaca
     5521 aggtacattt tcattaggac gtgtacaaac accgacttta tatctcattt atcaacgcca
     5581 ggaagcaata gaaaacttta aaaaagaacc ttttttcctg aataattcat agttttacta
     5641 atagtctttc ctaacattga tttaatagtc ttt
//
LOCUS       DACEUZ010000135         5668 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.135, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000135 DACEUZ010000000
VERSION     DACEUZ010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5668
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.135"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(14..766)
                     /locus_tag="IXG44_002298"
     CDS             complement(14..766)
                     /locus_tag="IXG44_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ1658995.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(1130..3796)
                     /locus_tag="IXG44_002299"
     CDS             complement(1130..3796)
                     /locus_tag="IXG44_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ1658996.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            4137..5588
                     /gene="cls"
                     /locus_tag="IXG44_002300"
     CDS             4137..5588
                     /gene="cls"
                     /locus_tag="IXG44_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAQ1658997.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
BASE COUNT         1641 a         1192 c          991 g         1844 t
ORIGIN      
        1 taaaaagaac ccttcacgag tcgtgaaagg ttcttttttt atctgcggac caaaggagca
       61 gtctataggt cagcttagtc gtactaagga tttctcgttt ttcactgaat agtttgcgaa
      121 tgtacctggt cacaacagga tcttttgcgt acgcgtcata gtatagcttc atcaattctt
      181 cagtaattga ttcctgtgta aaataagtaa aatctttttt cttcactttg aatccttcat
      241 caattaagta tgtttcaaat tgatccatta caactggcga tgtattagga tcttcttcta
      301 tcattcctag ttgcttttcg ataggataaa aaacatcatc gattgattcc actactctga
      361 ttatcacgca ccatttgctt gactttttca tcaatttttc taattgaata agatgtacca
      421 atgctggatt tgctgctgta aaaaccattt catatgtctc atttgattgg ataaattctt
      481 ctaacgactg tttgataaag gaaacatttg gcaactcatt tttttctgct tctttttttg
      541 cattttctag cattcctgaa gaaaaatcga tcaaatcata gcgattaatt tcttttgcta
      601 aggaaagaaa tctgccagat cctccagcaa cgtctgctgc tgtttcagca ggcaggactt
      661 tctccgtttt cagataattt tttacatctt cagcaatagt tacttgcgat tcttgctgga
      721 tttcataata ttcttcagca aattcatccc attctttttc attcatttga tttcctctaa
      781 ttttttgtta tatgttttca cttttcggaa taaatcattc tcctctttta tctattctat
      841 ctcttttgat gaataataaa atgattggct ttccgtctat cataaataaa aaaagggact
      901 gtgaccaaag tcgtgtcaaa gtccctcttt ccgaataaac agtgtccaat cagatgaccc
      961 tcagtattag ctcaaaatgc tgattttaca acttcttcct taaacggtgt tctcctgcca
     1021 actcttcggt cacaagtcac aatattcaac aaacatgaga agctgtttgt tgaaattgca
     1081 ccttgtgact cagaattaaa cggcaggctc acaacctcgt tgattcgttt tatttatttc
     1141 cttgtactcg tacaatgatc aatttagtga tttccatcaa aacaattgtc acaagtgcga
     1201 ttcccataca tgtaaagact tctgtcaatc caaatgctga tgggatagag aagacttctt
     1261 tcagtcctgg gatcattgtt aagcagtaaa gcgcgctaca tacaactaca gctagtaaga
     1321 cacttctgtt agagaagaac cctgcttgga tagctgtttg tgtgtttgaa cgtgctggga
     1381 atgtttgcaa tgtccgacac caaatcaatg ttgaaaatga catggctaca ccgaattcag
     1441 gtgaatgctg catgccaagc cattgagcaa agataacagc aatcccaatc agcacaccac
     1501 gatacgttac agaaatcaat gttttgtccg taaagattcc tgaatttgga tctcttggcg
     1561 ggcggttcat cgtagcaggt tcagcttttt ctgtacctaa tgcaattgct ggcagtgaat
     1621 cattgactaa attgatgaac aacagttgta atgctgtaaa tggattggac cagtcagcaa
     1681 ctaaggcaaa gatgatcgca atgatcgcac ctaaatttcc tgcaaataga taagaaatag
     1741 ctttcttgat attatcaaac acattacgtc ctacttctac tgcgctgaca atagaagcaa
     1801 agttatcatc cgtcaatacc atcgctgctg catcttttgc cacatctgta cccgttccca
     1861 tagcaattcc gatgtctgct tgtttcaatg ctggtgcgtc atttacacca tcacctgtca
     1921 tagctgagat atgaccttta ttttgccaag cacgaacgat acgaatttta ttctcaggtg
     1981 aaacacgtgc ataaacagtg actttttcta aaacttcgtt tagttgatct tctgtcaatt
     2041 catctagttc cgtcccggtc aaagccaggt ttccttcttc aaaaatcccg atttgttttg
     2101 cgatagcaac agctgttgtt ttgtggtcac cagtaatcat gattgttttg attcctgcac
     2161 ttcttgcttc tgttaccgcc tgagtcactt cagcacgtgg aggatcgatc atagcaagca
     2221 aaccaacaag gatcaaatcg tcttcatctt caaatgataa atcaaaatta tcaagtggac
     2281 ggtaagcaaa agctagtaca cgtaatgcgc gtgttgaaaa agcttcattt tgctcttgga
     2341 attttttcat caattcatcc gtcatcggaa cgacttcacc atttagcaat acttttttac
     2401 tgcgttggaa gacgatatct ggtccgcctt taacaaacaa gtaatttttg ttatcaattg
     2461 tgtgtccagt agacattaat tttcgatctg agtcaaaagg aagttctgct tgtcttgggt
     2521 agttgtttcg gatttcctga taaggctggt ttaatttttc actaaagtcg ataaacgcta
     2581 cttccgtagg gtcgcccaat ttagtaccgt cttcactgat cgatgcatca ttcgctaaaa
     2641 cactgatttc gatcaagcga cgttcgtcag ctgtccattt tgaagggtca tttgtgaaat
     2701 ccccagtatc gccattacct aaaaagtagt caacaactgt cattttgttt tgtgtcaatg
     2761 taccagtctt atctgtacag atgatacttg tcgaaccaag tgtttcaact gctggaagct
     2821 tccgaataat ggcatgacgt tttgccattt tattcgttcc caaagataac acgattgtta
     2881 cgatagactg taatgcttct ggaatagcag ctacggctac ggctacggca aacatcactg
     2941 cgctgacgat atctgcagtc atatctcctg taccttctcc aagataaata cgaaacagtt
     3001 ggatacctaa aatcaataaa gacaatgcca aaatagccaa acttagtttt ttactgaatt
     3061 tatccagacc tcgttgtaac ggagtgactt gctcagttgt tgattccaaa aggccagcaa
     3121 cttgtccgat ctccgtatta ttcccagttg aagtcactaa gaatttaccg cgtccgtaag
     3181 ttacgatcgt tccactgaat accatatttg tacgatctcc gattggtacc gtacttgtga
     3241 tatccgttac ttctttttct gcagaagtcg actctcctgt cagcatacct tcatctactt
     3301 ttaatgatcc cgcatctagt aaacgtccat cagcaggtac ataatcgcct gcgtctaaca
     3361 acacgatatc tcctaccaca agttcgtttg ccggaatcgt tttttcctta ccatcacgaa
     3421 tcactcgagc atcgggcgct gaaaggtttt tcaacgcttc taaagaacct tctgcttttt
     3481 ttgtttggac aacactgatt atagaattca acatcaatac tgcaaaaata actaagaatt
     3541 cgatgtagtc acccatgacc atctgaatca gtgcgacaat cagcaatacg ataaccattg
     3601 catctttgaa agtttctaag aacaatttcc aagttggctt cttcggcttt tcttttagct
     3661 ggttcaagcc atctctttct aatttttcct tcgcttgcgt ggtgctcaat ccatcggttg
     3721 agctctgcgt ttgctgcatc aacgagtcga tcgtttcttt atagaccgct tgttgtgctt
     3781 gttccttgtt ttccaaattt catcccccat tttttgatct tggccctagt cctgtctgaa
     3841 aaaaaagaga cctaagacaa atacgcattt gtcttaggtc tcaccatttc atacaatacc
     3901 agaaaaccat ttggttaact tactgttgat attatcgcaa caaacgttgc cggttactcc
     3961 cctaaggaga tattcaactg tactaatatc ctaccttatt aacctacaaa aagcaactac
     4021 tttagcccga tttacctaca ttttcatgaa aggagtgaaa agtatattca ccttggtagg
     4081 cttctttctt ctatcatgct atactaatgt tattgtatgt aaggaggaac atttttgtgg
     4141 tatctagtat tataaccgcc ctttatctat taaatgcact tattgctttg gtggctattt
     4201 tgatcaaacc ccgagatgta gcagccattt gggcatggct cttagtattg tttgcccttc
     4261 ctggggtggg ctttgtatta tatttatttt tcggacgtgg attaacggat aagaaaaaat
     4321 tctatctccg acaaagtgac ttgaaagaat tagaaaactt tcagtctttt agaggagata
     4381 ccattgaaca ttacgatcct gacatgggcg ataaagacaa acagcaattt gttgacttct
     4441 tctcttcatt aaatcgtatg ccgctgacaa gaatgaattc tgtcactctt ctcacagacg
     4501 gacaagagaa attggattca ctgcttcaag atctaaaaaa agccaaacat tcgatccata
     4561 tcgaatatta cgcatttgtg acagataata tcggccagca agtcttacat gttttagaag
     4621 aaaaagccgc agaaggcgtg gaagttcgaa tattatatga tgcatttggc tctcatggca
     4681 caaaagcaaa agatttcaat cgtctaatca aaaatggtgg acatgtccat acatttgtta
     4741 cctcacaaag ggcattactt cgtttccgat tgaattacca tgatcaccga aaaatcgttg
     4801 tgatcgatgg aaagattagt tataccggtg gtttcaatat tgccaatcaa tatgtaaata
     4861 caacaaaaaa attcggctat tggcgcgata cgcatatacg gattttcggt gccgcttctt
     4921 ctttgctcca gcttcgcttc ttaacagact ggaacgtctc ggtacctgaa gaaaaaaagg
     4981 tcggctatca tttgaattat ttctttaaaa aagcagatcg agatgaatct aagcttgctg
     5041 atacatccat ccagcttgtt tcaagcggac cgaataacga aagggaacaa atcaagcttt
     5101 catttatcaa attgattact tctgctaaaa aacgtgtttg gatacagaca ccttaccttg
     5161 ttcctgatga aagtgtcatt gctgctttaa aaatcgcaac tgcctctggt gtagatgtga
     5221 aaatcatgat tcccaacaaa ccggatcatc cttttattta tcgagcaaca caatattatg
     5281 ctcggcagct gatcaaggaa aatgtacaaa tccttgtcta tgagaacggc ttcctccatg
     5341 caaaaacatt gataatggat gatgaaatct gcatggtagg ttcagcaaat caagatattc
     5401 gtagctaccg attgaatttt gaaacaagtg ctgtcattta cgatcctgag tttttagaag
     5461 aacttgctac tcagttcaaa gaagatgaga cacattgttc atccatgaca actgaaacag
     5521 tcaaggaaat gtctaactgg ctattattca agcaacaaat ttctcgatta ttttctccaa
     5581 tcctataatt tttttattca agtagagaaa aatatactaa gattaaaggg actgtgacaa
     5641 aaatcttgtc acagtccctt cttccgaa
//
LOCUS       DACEUZ010000136         5643 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.136, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000136 DACEUZ010000000
VERSION     DACEUZ010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5643
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.136"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(188..1255)
                     /gene="rlmN"
                     /locus_tag="IXG44_002301"
     CDS             complement(188..1255)
                     /gene="rlmN"
                     /locus_tag="IXG44_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAQ1658998.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(1485..3932)
                     /locus_tag="IXG44_002302"
     CDS             complement(1485..3932)
                     /locus_tag="IXG44_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAQ1658999.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            complement(4121..5557)
                     /locus_tag="IXG44_002303"
     CDS             complement(4121..5557)
                     /locus_tag="IXG44_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ1659000.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
BASE COUNT         1485 a         1284 c          944 g         1930 t
ORIGIN      
        1 tgctaaaacc cccatcaagc aggctgtcat caaattgagg acatttcttt gcttgatgag
       61 ggtttttctt gtcaaaaaaa gctttatagc tttcgctata gaatcttttc tcttatttaa
      121 gttgaacagt tccttaatta agaaaactgc tccttttatg gtaattgaaa aaacgagtag
      181 gatacgatta tttggcaact gtttttttca tttgcttgct tcgcaattgt ccgcaagctg
      241 catcaatatc tgtaccatgt tctttacgta tcacacagtt tacgccgttt ttcttcaata
      301 catcataaaa tctcaataca tcagcttttg gactgcgaga atattgatca tgttcactga
      361 ctggattata tggaatcaag ttgacatatg ctaatttttt cttatctttt aatagatctg
      421 ctaattgttg cgcatgctct ggtctatcat ttacttgatt caacatgata tattcaaacg
      481 tgattcgacg gtttgttttc tctaaatact catccacagc ttccatcaat ttctcaattg
      541 ggaaacttcg gttgatccgc atgatcgatg ttcgcacctc attattcggc gcgtgcaagg
      601 aaatagccag attgacttgc aagccgttat tagcgaattc cttgattttg tgtgctaatc
      661 cactagtgga aactgtaatg tgccgagcac caattgccaa tcctttggca tcattgatga
      721 tatgcaaaaa gtccattaca ttgtcgtaat tatcaaatgg ctcaccgatc cccattacaa
      781 cgacatgtga aacgcgttcg ccaagattgc gctcatcaaa ataatgttgg accatcatga
      841 tctgtgcaac aatttctcca gcagtcagat cacggttctt tttgagcagc ccgcttgcac
      901 aaaacgtaca gccgatatta catcctactt gtgtcgtcac acaaactgac aagccatatt
      961 cttgtctcat cagtacagtc tcaatcatgt ttttatcagg gagttcaaac aaatatttta
     1021 ctgtaccatc cgaagcttct tggacgacca tctgttttag aggattgatg ataaaatgct
     1081 ccgataattt ttcgatcaat tgtttcgata gattcgtcat ttctgtgaaa ttacttacac
     1141 gtttttgata caaccattcc caaacttggg cagctcggaa ttttttctcg ccgttttcta
     1201 taaaccaatt gattaattct tcattcgtca agccataaat ggactgtttt tccaaagtat
     1261 tttccctctt tttctcaaag tttcgctaca tactatacat gattcatttc cttaatgcaa
     1321 tacttcacaa ataaatcgaa tagaacttta atctcacaaa aactaaaaag acaagacatc
     1381 ataccgtatt agaaaagatg gaaaaaaacc attttttctt ttggtatcat catgccttgt
     1441 ctttttattg gttattcgtt tttcaaccct tcatcacaca agttttacat gtcattatcg
     1501 tccggcaacg tttctgatgt ttcttcctgc tcaatgaata ctttccgagg cttgcttcct
     1561 tctgacgggc caacaactcc atgagcttcc agttcatcta ctaaacgtgc tgctcggttg
     1621 taaccgatcc gaaatcttct ttggagaagg gaaatacttg ccgtttgcat ttcaacgaca
     1681 agtgcttttg cttcttcaaa cagctcatct tgtggctgtt ctggagcacc tgtcgtttca
     1741 acttcatctg tcggcatcat tttctcttca taatgagctt cttgctgatc tgtaacaaat
     1801 tgaacgaccc gttctacttc gtggtcagaa atgaatgctc cttgcacacg gatcggttta
     1861 ttctcaccca ttggcaagaa tagcatatct cctcgcccta agagcttttc tgccccatta
     1921 gaatcaatga tcgtccgaga atctgtaccg cttgataccg caaatgccat tctggaaggt
     1981 acatttgctt tgatgattcc tgtgataaca tccacacttg gacgttgagt agctaggatc
     2041 atatgaatcc ccgctgcccg agccatttgc gctaaacgaa taatcgcatc ttcaacttca
     2101 ttgctggcta ccatcatcaa atcagctaat tcatctacga tcactacgat gaatggtaag
     2161 gttggatgtt tcgttccgtt ttccaagttt ttctgttgaa caaattcatt gtaaccagaa
     2221 atatttctta ctccggttgc cgcgaatttt tcatatcgtt cttccatttc ttgtactact
     2281 ttttgtaagg cttgcgctgc ttttctaggg ttcgtcacga ccggcgtcaa cagatgaggt
     2341 atgccgttgt acatattcaa ttcaaccatt ttcggatcga tcatcatcag ttttacttca
     2401 tggggcttag cacgcattaa tatacttgtg ataatcccat taattgctac tgacttccca
     2461 ctaccagtcg aaccagccac gagtaagtga ggcatcttcg aaagatcagc cgtttgtacc
     2521 cgtccagaaa catcacgtcc taaaggtact tctagtaatt tatctgggtg gtttggttgt
     2581 gcttcgatga tttctctaaa ggaaaccata ctgatctttc catttggtac ttcaatacca
     2641 atcagtgatt tacctggaat cggtgcttcc atccggacat ctttcgccgc taaagcaagc
     2701 gcgatatcat ctgttaagtt cacaatttta cttactttta cccctacagc cggctggact
     2761 tcgaattttg tgacggatgg tcctaagctg gctttgacga ccttggcatc gacaccgaaa
     2821 ctttggaaag tttgttctaa tacgccgata tttttttcaa tttttttata ctcatcactt
     2881 tgatccacag ttggtataga atcaagcaaa tcaactgtcg gtaattcata gtcttgattt
     2941 tcctgttctg cttcaatctc aaattctaat gaggtaccgt catcctctgg caactcatct
     3001 gaaaaacctt ctgctgtatt ggtctgtttt ggtgtatctg gtactggttt ctctagattc
     3061 gcaggatgga tattttcttg aaaactgtcg attggaacaa aacttagctg ctccggctct
     3121 gtattttctg gttcctgcca ttcatcagca gtattttccc tttgctggct caccgctttt
     3181 ttcttctcat attctgcagc ttctttttct gcagcttctt ttgcagctaa gcgccttgct
     3241 tctttcgctt ctgcgcgctg tttcattctc tcttcctttg ccgcttgctt tcgagcttgc
     3301 ttggcaggat caccttccaa taattcctgt actcgttctc caattgattg gataccattt
     3361 aacagctgat agccttccag attactgaac aggaatgctc ctcctgccag taatagcact
     3421 gctgctaaat aacttccagg ctgtgcaatc aagaagtaag taaaatgata aaggattgct
     3481 ccaatcatcc caccaccaac gttattgtct acttgacttt gttttatatc actggctaac
     3541 aaatcccatg tcgtccccat gatatttggt tccccagtat ggagtttgcc aaataaatgg
     3601 gcatgtaaca ataataagat accgaaataa aagagtatgc cgccaaacca gcgtcggttt
     3661 ttaccaattg aaaactctgt attcttaatg acaatccaaa atcctaatac ggctaataaa
     3721 agacagagga tttggtaagt atttcctgca atgatccgaa agccgtttgc aatcaatatt
     3781 cctaaaaaac ctaattttaa aaagccgaag acagcaaata aaatcaaggc aaacccaaca
     3841 ccaatcgtca gcatttgttc ctgctgctgt ttttgctttt tcgttttttt cgtctttttc
     3901 ctttttttag taggccgttt cttttgtgcc atttattatc cctcatttca tcaagtttca
     3961 ttatacaaaa aaaagtaagg gaactaaagt tttgtttgaa agaactgacg ctttctttca
     4021 tttttcagct tattttacag tttttcttaa tttctaaatg attttatcaa gaaaaggcac
     4081 ggccatatcc agccgtacct tcttgtttta agtatgtgaa ctaaaggttc tctccatttt
     4141 cttcaatcag tttttggtac cagtaataac tgtctttctt atatcgttcc aaagatcctt
     4201 cttgtgtttc gtcttgatca acatatacaa atccatatcg cttttgataa ccgttcagcc
     4261 agctaagcaa atctgtataa gaccaggtgc aatagcctag gacttctgct ccatccgaaa
     4321 ttgcttcttt gatggctttg acgtggcttt gtaagtaatt gattcgatag tcgtcatgga
     4381 tttgtttatc ttctgtcaac ttgtcatact ctcctaagcc attttccgta atcagtaccg
     4441 gcatgcggta acggcttgtg atacgacgca gtccgatcct taatccttct gggtcaatct
     4501 cccagtccca atttgttcgt tcgacaaatg gattctcaac ttttttatac attccaggaa
     4561 ctccagtttc ttctgatgaa cctttttttc ctgtcgtatt catttttcca gcaccaactc
     4621 cgtttaatgg attgaatgca ttcgtcgccg tttgatagta attgatccca agaaaatctg
     4681 gtttagcaga ttctagcaat tctgcatcgc cagcttcaat cgttggtgcc catccttttt
     4741 cttctaagaa cctcatggca gcaatcggat attttcccca catatagaca tccatccaat
     4801 aattggccat caggtcttct gcattttcag cagccagaac attttcagga ttactgtcaa
     4861 ttggataatt tggcgtatac gcaaagcttg gtccgatttt tccaggcatt tctaactcgt
     4921 gaaatttatt gatgacagat gcatttgcta aattagcaat atggttaact tcatacattc
     4981 gtttcgggtc tgtcacacct ggcggatgtg ctgctaacaa ataacctaat gaagtgaaga
     5041 tattttgttc attcaaacta atccagtaat ggactttccc gcggaatgcc tcaaataatg
     5101 tttctgcata atgagtgaaa tcttcaatga tctgacgtga ttcccaacca ttatattcat
     5161 cttgcaatgc ttgtggaaga tcccaatgat ataaagtcaa cataggttca atatcgtttt
     5221 caatcaattc gtcgatcaat ctctcataaa aagcgagccc agcttgattg acttctcctc
     5281 gtccattagg aaaaatccgt gtccaagcta cagagaagcg gtaagctttc agaccttgtt
     5341 ctttcattaa agcaatatct tctttataac gatgatagtg gtctactgcc aaatcaccgt
     5401 tcgttccttt aaatgtcttt ccaggtatcc gtacaaatcg atcccagact gattctcctt
     5461 tcccatcttc ttgccaagct ccttctactt gatacgctgc agaagcagag ccccacagaa
     5521 aatcttttgg aaatgaggta agttctttgt gttccattca tctcttcctt tcctataacc
     5581 ttcaatcgtt cctcttttag aataacacga aaccccttcc acaacaaaat aaaattatgt
     5641 aaa
//
LOCUS       DACEUZ010000137         5515 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.137, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000137 DACEUZ010000000
VERSION     DACEUZ010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5515
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.137"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(71..541)
                     /locus_tag="IXG44_002304"
     CDS             complement(71..541)
                     /locus_tag="IXG44_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HAQ1659001.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLDSNQAIISELTV
                     GGLVDSLALAEGFTGEDIYEMFSEVRQLKKGEKCIEVHHESPNVRFKRGDNEMIFKNH
                     VSDHTELYEIVNQILSGIFELGPYEFKEENGNPKLNEEWQETIETQRNNPHLFQ"
     gene            871..2511
                     /locus_tag="IXG44_002305"
     CDS             871..2511
                     /locus_tag="IXG44_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HAQ1659002.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAAAILEKYEDQITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQIILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     EGLTPGQIPFARKRSDFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQNLNREEITDIKEAVESAKWVPEYKSLED"
     gene            2665..3987
                     /locus_tag="IXG44_002306"
     CDS             2665..3987
                     /locus_tag="IXG44_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HAQ1659003.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRTVKPSIPQLGVGLMVSL
                     TFFILGQIGNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(4055..4486)
                     /locus_tag="IXG44_002307"
     CDS             complement(4055..4486)
                     /locus_tag="IXG44_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1659004.1"
                     /translation="MLREMRLEDAEALREINADQLGYDIPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKYAEKRGIGKALMTGVEEA
                     KKRGITTIRLNSAEYRVEAHQFYEHIGYHSDKMQKRFLKIL"
     gene            complement(4551..5387)
                     /locus_tag="IXG44_002308"
     CDS             complement(4551..5387)
                     /locus_tag="IXG44_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HAQ1659005.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQVIVLVVLLVNCYNAFVILQTLGESRLTQVKITETYC
                     SK"
BASE COUNT         1648 a         1099 c         1005 g         1763 t
ORIGIN      
        1 caggtttttc acgacctctg attcacggtg tttctttttc atcaacattt ttacgaaata
       61 ctgtggaaga ctattgaaat aagtgtggat tgtttctttg tgtttcaatc gtttcttgcc
      121 attcttcatt taattttgga ttaccgtttt cttccttgaa ctcataaggt cctaattcaa
      181 aaatcccaga cagtatttga ttcacgattt catatagttc agtgtgatca gacacatgat
      241 tcttgaagat catctcatta tcaccacgtt tgaacctcac attcgggctc tcatgatgaa
      301 cctctatgca tttttctcct ttttttaact gacggacttc tgaaaacatc tcataaatat
      361 cttctccagt aaaaccttca gcaagcgcca gactgtccac aagaccgcct actgtcagtt
      421 cagagattat tgcttgattg gaatctaatt ttaatgtata gcttgtcgct tctactacgc
      481 cacatgcatt gcaaccgcct tctgatagtt cgctttttct aatcaattga acttctttca
      541 ttttctcttc ctcctcttaa ttttctactt atgtattttt atcacctgac ataggtgact
      601 cctatcaacg ctatcatgtg cttttttcct cagaaatcct atttatccat tccatctatc
      661 ccttattctt ctacttttct ttctctagca tataagagat acgcatagta ttcaagcaag
      721 gattgtcgat ataaaaagaa caaatactgc tattttgatt tcttttggct atattacata
      781 taaataaaat ttatcatacc tgctcttttt gactatatca tcatctgact tctatacttc
      841 gtttgtatta aaacttagga ggaatcattc atgcttacag ggttaaactt attaaataat
      901 ccttttctaa ataaaggaac agcttttaca aaagaagaac gggcaaaata tggaatcact
      961 ggtatgcttc caagtactgt ccaaacactg gaacaacaat ctgttcaagc ttatggtcaa
     1021 tacttaagca aacaaacaga tttggaaaaa cgtatcttct taatgaatct attcaatacg
     1081 aatcggacat tgttttataa attgatgggc gaacatttag ttgaattcat gcctgtcgtt
     1141 tatgatcctg tcgttgcgga ttcaatcgaa caatacaacg aaattttctt gaaaccgcaa
     1201 gatgctgcat ttctttcaat cgacgaacca gaaaacatca aagcaagttt gaagaacgcg
     1261 gcagatggac gagacattcg tttgattgtt gtgactgatg cagaaggaat cttaggtatg
     1321 ggcgactggg gtgtgaacgg tgttgacatc gctatcggca aattgatggt ctatactgct
     1381 gctgctggta ttaatccagc acaagttttg cctgtatcga tcgacgctgg tactaataat
     1441 gaagaattat tgaataatcc cttatactta ggcaatcgtc atgctcgtgt agaaggcgaa
     1501 acttactatg aattcatcga tcaattcgtt caatcagcta ccgaattatt cccagaattg
     1561 ttattgcatt gggaagattt tggacgagga aatgctgcag cgatcttaga aaaatacgaa
     1621 gatcaaatca ctactttcaa cgacgatatc caaggtacag gaattgttgt cctagcaggt
     1681 gtattaggcg gattgaatat atccggtgaa caattgaaag atcaaataat tttgacattt
     1741 ggtgctggta ctgctggtgt gggtatcgca aacatccttt tagatgagat gatccgtcaa
     1801 ggtgtacctg aagaagaagc acgccaacat ttctatcaag tagataaaca aggtttattg
     1861 tttgaagata cagaaggatt gactcctggt caaatcccat tcgctcgcaa acgctcagat
     1921 ttcactaatg ctgatgaatt gacaaactta gaagcagttg taaaagcgat ccatccgaca
     1981 atcatgattg gtacttctac ccaacctgga gcattcactg aagaaatcgt gaaagaaatg
     2041 gctgctcata caccacgtcc gatcatcatg cctttgtcta atccaacaaa attggcagaa
     2101 gcaaaagcaa aagatttaat agaatggacg gacggaaaag cattagttgg aacaggtatc
     2161 cctgctgctg atgttgaata taatggtgta acttaccaaa tcggtcaagc aaataatgct
     2221 ttgatgtacc caggtttagg acttggtttg attgcttcaa cagctacacg agtaaatgct
     2281 gaaatcattt ctcaagctag tcgagcatta ggcggaatcg ttgatgtaac aaaaccagga
     2341 gcagctatct tgcctccagt agcaaaaatc actgaatttt ctcaaacgat tgctgaaact
     2401 gtcgcaaaat cagttgtcgc acaaaacttg aatcgtgaag aaatcacgga tatcaaagaa
     2461 gcagttgaat ctgctaaatg ggttcctgaa tataaatctt tagaagatta agatcgttat
     2521 agtaaaagaa catacagaaa ctttgtatga cgtctaaaaa gaaaaaagcg actgcggcaa
     2581 aacagcggcc agccaactcc tgctcttttg tcgcttttct ttggcttttt tcatatgata
     2641 cagaataaga aaggatctgt aaaaatggca caaaaaacaa caactgcaac tgcagttgag
     2701 gaaaagaaaa aagaaggttt ttggtccaca aaaatcaccg gcgtaagttt gccactttat
     2761 ttgatcatgg tccttgtcct gatcattgca atggctttag ggaaattacc gacgaatatg
     2821 attggaccaa tcgcagtttt ggttattttc ggtaatttat ttcattttat cgggacaaaa
     2881 atccctatcg tcaaaagtta tttgggtggt ggttccgttt ttgcgatctt cgcttctgct
     2941 gcgattgcta catttgggat cttacctgaa tatgtagtga aatccacaga aaactttgtc
     3001 agcaatatgg gctttttgga cttttatatt gctgccttga ttactggtag catccttggt
     3061 atgaaccgta atctattgat gaaagcttct gttcgtttta ttcccgttgc acttatttca
     3121 atggtcgtct ccttttttgc cgtaggactc gtgggcatgc ttatcggaaa tggttttgca
     3181 aattccgttt tatacatctc attacctgcg atggctggtg gtgttggagc cggagccgtt
     3241 cctttatcaa cgatttatgc aaatgtttta ggtacggatg cttcttctat catttcccgc
     3301 ctgattcctg cgacagcaat gacaaatgtc ttagctatca tcggtgcagc attagttgct
     3361 cgtgctggac aatcaatgcc aaaattcaac ggcaatggta aattaatgga aaaaggcgat
     3421 cttggcgatg gaaaacctcg aactgtgaaa ccttccattc cacagttagg tgttggtttg
     3481 atggtatcat tgactttctt catcttagga caaattggta actattttgt tcctaaagtc
     3541 catgcttacg catttatgat tattatcgtt gtcatctgta aaatcgccaa cctattacca
     3601 gagtattatg aagatgcagc tatcatgttc aataacttga ttgtcaaaaa cttgacagca
     3661 gctgttcttg ccggtatcgg tattgcgttg ctgaatctga atgtattagc ttctgctttg
     3721 acatggcaat ttgttgtctt atgtctgaca agtgttatcg cgatttcagt tgtttcagga
     3781 tttgtcggtc gtttgtttgg tttatatcca attgaatcaa ctattactgc tggcttatgc
     3841 aacaactcga tgggcggaac aggaaatgta gccgtcttat ctgcagctaa tcggatggaa
     3901 cttattgctt ttgctcaaat gggcaatcga cttggtggtg ccatcgtttt gatcatctct
     3961 ggtttcttga tgcaattact ttcttagtca aaaaaacaaa aatacctgat catcaagtat
     4021 cggcttggtg atcgggtatt tttttgataa agatttacag gattttcaaa aatctttttt
     4081 gcattttatc ggaatgataa ccgatgtgtt cataaaattg gtgtgcttct actctgtatt
     4141 ctgcagaatt aagccttatt gtcgttattc ctcgtttttt cgcttcttct acgccagtca
     4201 tcaaagcctt tccgatgcct cttttttcag catatttaga cacagcaagt gccataatat
     4261 tgaacattgg atcgctaaaa atcgattcat aggactccgc ttgtacatag ccagctgctt
     4321 ttttagaagt aggatcttca taaaccaaga aaaaattttt ctctggctcc ttcagcagtt
     4381 tcttcatttg tctcaccgta acggttaatg gtatatcata ccctaattga tccgcattga
     4441 tttcacgtag tgcttctgca tcttctaaac gcatttcccg taacatccaa atcaccagcc
     4501 aatttctgat tttttaaaat gaattttttt atcatagtat actctttttt tcatttagaa
     4561 caatatgttt cagtgatttt gacttgcgtt agtctagatt ctcctaatgt ttgtaagatg
     4621 acaaaggcat tgtaacaatt gaccaaaaga accacgagga caattacttg aaaaataaag
     4681 gtcacttttt ttggagacaa aaccctgcta aatcttgctc cccacaaacc tccaataaca
     4741 gcagcgggga taacataaaa caacatagat aaatcaaagc gcataaagct acttgttaaa
     4801 gcaatcgtta ccaattttgc cagttgagaa aagaaaatcg tagatagaga atatagtgtt
     4861 gcttctttta ttggcaatgc aaacatcagc atcaacaaag aaacattgac tggtccacca
     4921 ccaataccta aaaaactagc taaaaagccc agaacccccc cacatataag gtaccaagtc
     4981 catgacgtca aatgaaattt tggctgatga tgcttggtgt agaagaacgc aaaaaccaag
     5041 gtcaataccg tcaaaaaaat ttggatcagc tgcacatgct tctcgttttc aaataactgt
     5101 aaaaaaactt cgaatacaat gttgccgact actcctccaa caacagctcc actagatacc
     5161 caaccgacaa tctgccaatt cagtgatttc ccgcttgata cttgtcgcac agtcgaaaca
     5221 atggacatcg tgaataccgc aactgttgag taaaaagaaa tccctgcaac agaatgggcg
     5281 ccgataagat caagaatcgg tttgatcaac acgcctccac ccatcccgga aatagcgcca
     5341 actgtgttcg ccagaacgat caccacaaaa taaatcagac caatcatctt gtatttcctt
     5401 tctcttgttt atatctttta gcgattgtta ctcttttctt ttattataca gtaaaagaaa
     5461 caaacgaggc ttcgaaagct tttctacttt ttaaggaaat ttagtgctaa acgaa
//
LOCUS       DACEUZ010000138         5465 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.138, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000138 DACEUZ010000000
VERSION     DACEUZ010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5465
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.138"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            132..1106
                     /locus_tag="IXG44_002309"
     CDS             132..1106
                     /locus_tag="IXG44_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ1659006.1"
                     /translation="MRQLHKEQIETAMAALKEKTIDHVYFTACGGSQAVLMAGQYLFD
                     KELTVPCHVYTANEFNYAIPKGLSENSLVITCSHSGTTPETVEATKKAREFGAMTICL
                     SNEEGSPLWEAAEYPVHYDWGKDVDASDLNKGILYGLLFNILLVLDGSKKWETCLNEF
                     DKLNDLVIAEKSKFEITAKEWGKNQKRDKIIYTIGSGINYGEIYSTAACWFMEMQWIN
                     SNAIHSGEFFHGPFEITDYDVPFILVKSIGDTRFLDQRVEDFTKKFSNKLMVLDQADF
                     DLESIDSVAKEYIAAVLSGVVIRQMVEAIAFERGHSLDVRRYMWQMKY"
     gene            1088..1549
                     /locus_tag="IXG44_002310"
     CDS             1088..1549
                     /locus_tag="IXG44_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAQ1659007.1"
                     /translation="MANEILINRRLQKMANQLFHIVITTHSTLCEGYLKATKLILMND
                     ATGISAVPFKENTSPDDFEESMKEIISQHHLQPLIILTDLIGGTPNNVSIKQLNNNRI
                     QIIAGINLPLLLELLMAQSAGKSIEEIDFKELLNNSQKSMVHINSLLKDVS"
     gene            1599..2063
                     /locus_tag="IXG44_002311"
     CDS             1599..2063
                     /locus_tag="IXG44_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1659008.1"
                     /translation="MIKLVRVDHRLLHGQVIFSWTKQYDINHIIVADNKVPGDPMSVM
                     ALTIAKPADCKLDIVKISDVKGIMETSPNDNYMILIKGPKEAFELVQNLPEIKEINFG
                     GVSKKSESKQYGKAVFLTPEELEATKKILDQEVKIFVQQVPSSSIENVDFSK"
     gene            2077..2826
                     /locus_tag="IXG44_002312"
     CDS             2077..2826
                     /locus_tag="IXG44_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1659009.1"
                     /translation="MLLKAILLCLVGVVAVIDSRLIGRQNIGRPLILSTLAGLVLGDL
                     HTGIVLGASLELISMGFVSIGAAGPPNMQLGSIIATAFAILTNSSTETALTIAIPVAV
                     AGEFMSIIMRMIIAQFGHAADKAIENNKFRKAQIIHIYWSFIFNAFVYFIPIFLTVYF
                     GADVVADLVDKIPEMITDALTVSGNMLSALGFAMLLSTMLSKKLYPYFIFGFFIVAYS
                     GLSLIGVTIFAALIAFVMDQVKYGKREEAHG"
     gene            2826..3656
                     /locus_tag="IXG44_002313"
     CDS             2826..3656
                     /locus_tag="IXG44_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ1659010.1"
                     /translation="MENSTEKKRLITKKDLHKMCLRSWTIMCSWNYERQMHMGFMYGM
                     APILNKVYKNDEEKKREAYTRHMAFFNCTPQMTPFIMGLCASMEEQNAQAEEGNFNTE
                     SISMIKTSLMGPFAGIGDSFFQGTLRIITFGIGLSFAKQGSILGPILAVGLFAIPSIL
                     AVFYGGKLGYESGNKYLTKLYQEGLMDRVMQFASIIGLAVVGGMVASMVPIVTPLKFT
                     TGGTELVLQDLLDSIIPGLLPLVCTLGIYKLIKKNVSTNMLLLGCILAGFVLSIIGIL
                     "
     gene            3656..4303
                     /locus_tag="IXG44_002314"
     CDS             3656..4303
                     /locus_tag="IXG44_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ1659011.1"
                     /translation="MIKALIFDMDGVLIDSEIMYQKWVFNFLKQENLSYPLYSYQKKI
                     GTTASIFDDIEKYNEGCNSLELKGKFTKYWKSQKINYLDIFPNILIEQLEWLKKKKIK
                     MAIASSSPMDTINEVVTTCKIESYFDCLISGRDLPESKPNPTIFLKASEQLRIPVEEC
                     LVIEDSYNGIKAGKRANMKVLAIKDKRFSQDVSLADDVIYDIKYLRQEVQVKFGI"
     gene            complement(4401..5177)
                     /locus_tag="IXG44_002315"
     CDS             complement(4401..5177)
                     /locus_tag="IXG44_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1659012.1"
                     /translation="MNFQDNRPLYLQVEASIRNDIREKKYLPGEQLPTEEELCKIYGV
                     SKITIRKAFKLLTESGTVERLRGKGTFVTQKKESLKLAGVHGFSDSLSTRGHKIRYTV
                     LHSKIIKADEFLANKLQINVTDRVYNIKRLMWEDGAPMGIDNFFASEEKFPTLFEKGS
                     QYTSLYKLLEDDYDVKATSSIMEINGLSATPELSDLLQCIVGDPLFILEKVGKDKNNQ
                     PFHYSQSTVRCDRISYVIEVTENITLDTVHKNDSDPKVID"
     gene            5259..5402
                     /locus_tag="IXG44_002316"
                     /pseudo
     CDS             5259..5402
                     /locus_tag="IXG44_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608918.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
BASE COUNT         1874 a          738 c          971 g         1882 t
ORIGIN      
        1 attttattat ataaaattgt tataaaaaat ttttttgaaa aatagtattg caaaataaag
       61 caatttcata gataataggt atcgtaaagt tatataactt tataatacct attagagtga
      121 gggataaaga aatgagacaa ctacacaaag aacagattga aacagcaatg gcagcgttaa
      181 aggaaaagac aattgatcat gtatatttta cagcatgtgg gggttcacaa gccgttttaa
      241 tggctggtca atatctattt gataaagaat taactgtacc ttgtcatgtt tatactgcta
      301 acgaatttaa ctatgctata ccaaaaggat tatctgaaaa ttctttagtt atcacttgtt
      361 cccattctgg cacaacacca gaaactgtag aagcaacaaa aaaagcaaga gaatttggag
      421 caatgactat ttgtttatct aatgaagaag gatctcctct atgggaagca gctgaatatc
      481 ctgttcacta tgattggggt aaagatgttg atgctagtga tttaaataaa ggaattcttt
      541 atggtttatt atttaatatt ttacttgttt tagatggctc taaaaaatgg gaaacttgct
      601 tgaatgaatt tgataagtta aatgatcttg ttattgctga aaagagcaaa tttgaaataa
      661 ctgctaaaga atggggtaaa aaccaaaaac gagataaaat tatttacaca attggtagcg
      721 gtattaatta cggagaaatt tactccacag cagcttgctg gttcatggaa atgcaatgga
      781 ttaattctaa tgctattcat tctggggaat ttttccatgg tccttttgaa atcactgatt
      841 atgatgtacc attcatctta gtgaaaagta ttggagatac aagattttta gatcaacggg
      901 tagaagactt cactaaaaaa ttcagtaata aattaatggt tttagaccaa gctgattttg
      961 acttagaatc tatagatagt gtagcgaaag aatatattgc agctgtactt agtggcgttg
     1021 ttattcgcca aatggtagaa gcgattgctt ttgaaagagg ccattcctta gatgttagac
     1081 gctatatgtg gcaaatgaaa tattaattaa taggaggttg caaaagatgg caaatcaatt
     1141 atttcacatt gtgattacaa cacattctac cctttgtgag ggttatttaa aggcaacaaa
     1201 attaatttta atgaatgacg ccacaggtat tagtgctgtt ccatttaaag aaaatactag
     1261 tccagatgat tttgaggaga gtatgaaaga aataattagt cagcatcatt tgcaacctct
     1321 aattatttta acggatttaa ttggaggaac acctaataat gtttctatta aacagttgaa
     1381 caacaataga attcaaatta ttgctgggat taatttacct cttttactgg agttattaat
     1441 ggcccagtca gctggtaaat ctatagaaga aatagatttt aaggaattac tgaataactc
     1501 tcaaaaaagt atggttcata ttaattcgct tttaaaagat gtcagttaag gtggcaattt
     1561 ctattctata aattttaaaa attgaaggag gaaatagaat gattaaatta gtgcgtgtag
     1621 accacagatt acttcatgga caagtaatat tttcttggac taaacagtat gatattaacc
     1681 atattattgt ggctgataac aaagttccag gagatccaat gtctgtgatg gctttgacaa
     1741 ttgctaaacc tgctgattgc aaattagaca ttgttaaaat tagtgatgtt aaagggatta
     1801 tggaaacatc cccaaatgat aattacatga ttttaattaa aggacctaag gaagcttttg
     1861 aattagttca aaatttacca gaaattaaag aaattaattt tggcggtgtg tctaagaaaa
     1921 gcgagtccaa acaatatgga aaggcagtct ttttaactcc tgaagaatta gaagccacaa
     1981 aaaaaattct tgatcaagaa gtgaaaatat ttgtgcaaca agtcccatct tcaagtattg
     2041 aaaatgtaga ttttagtaag taagggggga aaataaatgt tattaaaagc cattttatta
     2101 tgtttagttg gtgtggtagc tgttattgac tccaggttaa ttggtcgaca aaatattggt
     2161 agaccactaa ttttgagtac acttgccgga cttgtgctag gtgacttgca tacagggatt
     2221 gttcttgggg catctttaga attaatttct atgggattcg tctcaattgg ggcggctgga
     2281 cctcctaata tgcaacttgg tagtattatt gccactgctt ttgcaatttt aactaatagt
     2341 agtacagaaa cagccttaac aatagctatc ccagtggctg tagctggtga atttatgagt
     2401 attatcatgc gcatgataat tgctcaattt ggacatgctg cagataaagc cattgaaaat
     2461 aataaattta gaaaagctca aattattcat atttattggt catttatttt taatgctttt
     2521 gtttacttta ttccaatctt tttaacagta tattttggtg ctgacgttgt ggcagattta
     2581 gtagataaaa ttccagaaat gattacagac gccttgactg tctctggaaa tatgctttcc
     2641 gcattagggt ttgcaatgct attaagcaca atgttgagta aaaaattata tccttacttt
     2701 atttttggat tctttattgt tgcctattca ggtctttcac taattggcgt gacaattttt
     2761 gcagctctga ttgcctttgt aatggatcaa gttaaatatg gaaaaagaga agaggcgcat
     2821 ggttaatgga aaatagtaca gaaaaaaaac gtttaatcac aaaaaaagat cttcataaga
     2881 tgtgtcttcg ttcatggaca atcatgtgtt cgtggaatta tgaaagacaa atgcatatgg
     2941 gatttatgta tgggatggcg ccaattttaa ataaagttta taaaaatgat gaagagaaaa
     3001 aaagagaagc ttatactcgt catatggcat tttttaactg tacgcctcaa atgactccat
     3061 ttattatggg actttgtgct tctatggaag aacaaaatgc tcaagcagaa gaaggaaact
     3121 ttaatactga atctatctcc atgattaaaa ccagtttaat gggtcctttt gctggaattg
     3181 gtgattcatt tttccaaggt actttaagaa ttattacatt tgggattgga ttgtcttttg
     3241 ctaagcaagg tagtatttta ggaccgattt tagctgttgg cctttttgct ataccttcta
     3301 ttttagctgt tttttacggc ggtaaattag gatatgagtc tggcaataaa taccttacta
     3361 aattgtatca agaaggtctt atggatcgag taatgcaatt tgcttctatc attggtttgg
     3421 ctgtagtagg tggaatggtg gctagcatgg tgccaattgt caccccttta aaattcacta
     3481 cagggggaac agaacttgtt ttacaagact tacttgattc cattattcct ggattattac
     3541 cacttgtatg tactttggga atatataaat tgattaagaa aaatgttagt acaaatatgc
     3601 tcttattagg ttgtattttg gcagggtttg ttttaagtat aataggtatc ttataatgat
     3661 taaggctcta atttttgata tggacggagt tttaattgat agcgagatta tgtatcaaaa
     3721 gtgggtattt aattttttaa aacaagaaaa tttaagctat cctttgtatt cttatcaaaa
     3781 aaagattgga actacagcaa gtatttttga tgatatagaa aagtacaatg agggatgtaa
     3841 cagtttagaa ttgaagggaa aatttactaa atattggaaa tcgcaaaaga ttaattactt
     3901 ggatattttt cctaatatac taattgaaca gcttgaatgg ttaaaaaaga aaaaaataaa
     3961 aatggcaatt gcttcttcca gtccaatgga tactataaat gaagttgtta ccacttgtaa
     4021 aatagagtca tattttgatt gtttaattag cggaagagat ttgccagaaa gtaaacctaa
     4081 tcctactata ttccttaaag cttcagaaca gctcagaatt ccagttgaag aatgtctggt
     4141 gatagaagat tcttataatg gtataaaagc tggtaaaaga gcaaatatga aggttcttgc
     4201 aattaaagat aaacgttttt ctcaagatgt aagtctagca gatgatgtta tttacgatat
     4261 taaatatctg cgacaagaag tacaggtgaa atttggaata taaataagtt ttttgatgta
     4321 tttagagaat attagtaaaa ataaaaatca tgcccaaatc taatcaaaat tgattgcttt
     4381 gggtatgatt tcttttaata tcaatctatt actttaggat ctgaatcatt tttatgcacc
     4441 gtatctaaag tgatattttc tgtgacttca ataacataac taattctatc acaacgaact
     4501 gtggattgag aataatgaaa tggttgatta tttttatctt tgccaacctt ttctaaaata
     4561 aatagcggat ctcctactat acattgcaat aagtcggaaa gctcaggtgt tgcagataaa
     4621 ccgtttattt ccataataga gctcgtagct ttgacatcat agtcatcttc tagaagttta
     4681 tataatgaag tatattggct acctttttca aataaagttg gaaatttttc ttctgatgcg
     4741 aaaaaattat caattcccat aggggcaccg tcttcccaca ttaaacgttt aatattgtaa
     4801 actctatcag ttacattgat ttgtaattta ttagctaaaa actcatcagc ctttataatt
     4861 ttagaatgta aaacagtata tctaatttta tgtcctcggg tagaaagaga atcgctaaat
     4921 ccatgaactc cagctaattt taaactttct tttttttgag ttacaaaggt ccctttcccc
     4981 cttaatcgtt caactgttcc actttctgtt aaaagtttaa aagcttttcg aatggtaatc
     5041 ttacttactc cataaatttt acataattct tcttctgtag gtaattgctc tcctggaaga
     5101 tatttttttt cacgaatatc gttacgtata gaagcttcta cttgtagata taatggtcta
     5161 ttatcttgaa aattcatagt acactcccta aaaagttata cttcattata tcacaattta
     5221 ttaaaataaa agattaagat ataaatataa atgaactagt ctttgactaa ccaatgcccg
     5281 cttttgaaag aaaacaaatt cattctgttt taagtaaaaa tccttatgtt accactcatt
     5341 ctgaagggga tgagccttat cgctacttgg ttgtggaacc aagtgataag acaattttat
     5401 aaattcaata ttaattaaaa taaaaacaaa caaagtagat tattttactt tgtttgtttt
     5461 tttat
//
LOCUS       DACEUZ010000139         5339 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.139, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000139 DACEUZ010000000
VERSION     DACEUZ010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5339
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.139"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(19..414)
                     /locus_tag="IXG44_002317"
     CDS             complement(19..414)
                     /locus_tag="IXG44_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008688205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1659013.1"
                     /translation="MKNKGQIWYIGYIVSLFLIVTILVTDFPKMVDIGLAILFSAVFG
                     VSHVQILHDKMIKNDADYKINVMDERNIIIKEKAGNITNMFNTILLGVATVIFICLDY
                     IIPAIIVGSIIAIQPIILIIISSVLEKKM"
     gene            complement(404..616)
                     /locus_tag="IXG44_002318"
     CDS             complement(404..616)
                     /locus_tag="IXG44_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005814946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1659014.1"
                     /translation="MKNRLEELRKQRGIKQEDLASALEVSRQTIGSLENGRYNPSIIL
                     AFKIARYFQMSIEEIFIYEEESEDEK"
     gene            1688..2290
                     /locus_tag="IXG44_002319"
     CDS             1688..2290
                     /locus_tag="IXG44_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1659015.1"
                     /translation="MEDIILRDRLTDPLKNSLANVIYDILREADKVYSNYNGKVSWVI
                     YSFLIKEFFKEQINNSYELGYHFTVYYEDIIKRLEDLEWYAYYDLLEVFASAKILDKK
                     KREEVNKILESENSGYRLSESNEFIPITDDISIKSINAVSESPFNYAKNHIHKSLTYI
                     SEKENQDYNTVVREAISAVESCLIEFSGLPANKKILWELL"
     gene            2645..2881
                     /locus_tag="IXG44_002320"
     CDS             2645..2881
                     /locus_tag="IXG44_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003125429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HAQ1659016.1"
                     /translation="MNIKKRKIREVGNSIVVTLSKESLLQKGLKPGDTIFIDEDKMME
                     AIVKEESKIDLEIDMYVNQALSEYDAAFKELVEK"
     gene            2878..3291
                     /locus_tag="IXG44_002321"
     CDS             2878..3291
                     /locus_tag="IXG44_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAQ1659017.1"
                     /translation="MIYLSEKDIVKLNVIQIKKYSPRETIGVIDKNALSMAVNQPKQH
                     IFGKDLYPDKFSKAAVLLINLIKKHPFHNANKRTAFLAAYVFLKLNGYSLKMKNQEVV
                     EFVVEIATYQGDFDDLKEATTDILKKNSTKTDSKK"
     gene            3810..4760
                     /locus_tag="IXG44_002322"
     CDS             3810..4760
                     /locus_tag="IXG44_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HAQ1659018.1"
                     /translation="MERNSENKMTGRNPKVFISYSWENKENKEWARELADKLLENGID
                     AYIDQYDLSLGDRLPQFMEQQISDSDYVLIICTPEYKKKANNRTSGVGYEGHIISAEL
                     LSGKNERKFIPIIKEGRASEVLPTFLAGKLGIDLSDSQHYDENLDDLITTIFGVKRKP
                     ALGDIPRHIKGSPLLPIEHNENKDIEILGIITDKVTVPKMDGSRGSALYKIPFRLNRV
                     PSQLWKELFVRNWNSPPRYTTMHRPGIASVVDDEILLNGTTIEEVREYHRETLKLCIE
                     KSNTQEKKILLEEEQKKERENERVRSHKESVQKLADELEF"
     gene            complement(4757..>5339)
                     /locus_tag="IXG44_002323"
     CDS             complement(4757..>5339)
                     /locus_tag="IXG44_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1659019.1"
                     /translation="SIDQNEQRQIEEMRKFGAEKIFIEKQSGATITHRPIFQEVLDFV
                     RDQDIFIVEAIDRLGRNYDEIIDSVNYLKKKNVRLIITNLPIMAEAIGNPLLDKFIKD
                     LIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANTKDPQRRLVYRSIV
                     QDQENGVAISKIATDYNVTRQTVYRIKKELTIN"
BASE COUNT         1908 a          760 c          902 g         1769 t
ORIGIN      
        1 ttgtatgaac ctttatttct acattttttt ctcaagcaca ctactgataa tgattaaaat
       61 aatcggctgg attgcaatga tacttccgac aataatagct ggaatgatgt agtctaaaca
      121 aataaatata actgttgcca cccctaacag tattgtgtta aacatatttg ttatatttcc
      181 cgctttttct ttgattatga tattccgttc gtccataaca ttgattttgt agtctgcatc
      241 atttttaatc attttatcat gcaaaatttg aacatgagaa acgccaaaaa cggcactgaa
      301 taaaatagca agtccaatat ctaccatttt aggaaaatct gtaaccaaaa ttgtcacaat
      361 caaaaacaac gaaacaatat aaccgatata ccaaatctga cccttatttt tcatcttcac
      421 tttcctcctc atagataaaa atttcttcaa tgctcatttg aaagtacctt gcaattttaa
      481 atgccaatat aatagaggga ttataacgtc cattttctaa agaacctatt gtttgacgag
      541 atacttcaag agcagaagct aaatcttctt gtttaattcc tctttgtttt cgtaattctt
      601 ccaaacgatt tttcacaaca tgctcctttc taaaaaatgg aaagtttact ttcctttcgt
      661 gcttttataa tactcctgcg agcacaaaat gtcaaggaag ctttccatat aaattttgat
      721 tttaatgttt tagttactgt ttttatgaca catgaacaca cttattctta tttcctctta
      781 tctgctatca acagaaaaga aaaagccctg tcaagagccg attagatcaa gccagtcgag
      841 aaacgatgtt agaccaaatt gattttgggt aatgaatgtt tagaacttag tagtcatcca
      901 aaaacgacta ctttttttgt tgaatcgtcc aaggacgatt cgatgctttt gacttgtaag
      961 aacaggattg aatgaaacca tactgttcgc atatttttag tggatgaaca aacaaaatac
     1021 gagagatttt ttgttcgttc atccatggtt ttagaaaaaa gagggacgat tttggaagaa
     1081 gaaaatcgtc tctttttttt cttctttttg tatgacaaaa agaaagatct tttgcccatt
     1141 ttatttttat aaaatgggta ggtggcgttc gcgtaaagca aatcgataca atccaatggt
     1201 tttggcgaat cagatttacc attctaggct tgtttaatac agacatcgat tgttattttg
     1261 agaatttata tccttattgg caatgaaatg gatattctcc ataccaatag aaatcttcaa
     1321 gataattttc ggaaatatat aagagttaat caattatgat catatagtag gtaaaaatta
     1381 ggacagcctt caattatatg ggaaaccaat ataatcaagt aattattcgg gtaacacaaa
     1441 tactagagta ctcatttctt ataactgttt tacacctatt agggatcagt attattaata
     1501 gatctagata aattaatacg taattatata ataatttatg tttgtgccat taaacaatct
     1561 taacagatat ttcattttat ttatgtaaaa caacacgaat aattattgct tcgtaaagtt
     1621 atatgagtat aagttaattt actggaggta gtgcaatgct tttttctgaa agatttggtt
     1681 ttaaaaaatg gaggatatca tactgagaga tagattgaca gaccctctta aaaatagttt
     1741 agcaaatgtg atttatgata tattacgaga ggctgataaa gtttatagta attataatgg
     1801 aaaagtctca tgggtaatat attctttttt aattaaagag ttttttaaag aacagataaa
     1861 taatagctat gagttgggtt atcattttac tgtttattat gaagatatta tcaagaggtt
     1921 agaagacttg gagtggtatg cttattatga tttattagag gtttttgcaa gtgctaaaat
     1981 cttggataaa aagaaaagag aggaagtaaa taaaattctg gagtcggaaa attctggtta
     2041 cagattaagc gaaagcaatg aatttattcc aatcacagat gatatttcta ttaagtctat
     2101 aaatgctgta tctgaatcac catttaatta cgcaaaaaat catatccata aatctcttac
     2161 atatataagc gagaaggaaa atcaagatta taatactgta gtgagagaag caattagtgc
     2221 tgtcgaaagt tgtcttatcg aattttcagg attaccagct aataaaaaaa tactttggga
     2281 actgctataa aagcaattaa agacaaatcg aataacaatg tcgatcttac atttttaaaa
     2341 ccatttgaga caatgtatgg tttggcgagt aataatggaa tacgacatgc agaaaatgaa
     2401 aaaacaattg tctcagattt acctgatgca gttttggtgt taacaacatg ttcagccgta
     2461 attaattatt tgggagtaaa aatagcatat gactccaata aatcatgaaa agttaaaatt
     2521 catccaagag ctacattata actattaatt tccaactctt taaaagttta gtaattgaag
     2581 gaagtaataa gggtgattga acaatacgga tatacggtgt atacttatcg taggagttga
     2641 tgatatgaat attaaaaaac gcaaaatcag agaagtaggt aattcaatag ttgtaacttt
     2701 gtcaaaagag agtttattgc aaaaaggttt gaaacctgga gatactattt ttattgatga
     2761 agataaaatg atggaagcta ttgtaaaaga ggaaagcaag atagatcttg aaattgatat
     2821 gtatgttaac caagctttat ctgagtatga tgcagctttc aaggaattag ttgaaaaatg
     2881 atctatctaa gtgaaaaaga tattgtaaaa ttgaatgtta ttcaaataaa gaaatattca
     2941 ccgcgagaaa caatcggagt cattgacaaa aatgcgctga gtatggctgt aaatcaacct
     3001 aaacagcata tttttggaaa agatctgtat cctgataaat ttagcaaagc agctgttctg
     3061 ctaataaatc tgatcaaaaa gcatccattt cataatgcta acaaacgtac agcattttta
     3121 gccgcgtatg tttttctcaa gctaaatggc tattcactaa aaatgaaaaa tcaggaagta
     3181 gtagaatttg tagttgaaat tgctacgtat cagggtgatt ttgatgactt aaaagaggca
     3241 acaacagata tactaaaaaa gaattcaaca aagaccgatt caaaaaagta gaagcaaact
     3301 agggattcag ttaaatctga agatttagtt tgttctttat tttagtttac ataagataca
     3361 ttatgcgaag ttcattattt taaaagctta aataataaac ttttttataa taccaattta
     3421 tttatgtaaa ccaatgacct taatcgctta agcaagcatg ttatgtaata ctctactctc
     3481 tccaaaacta atgactatct attttcaaag aagctagaat gattatctga tcaaatgata
     3541 atttatattt aattcctggt ctactgctct tcttttaatc aattaatgtc taacgtactg
     3601 tacacgataa agtaggaaga cttattttca acttcggatt cattttttta attcggtgaa
     3661 aatcactaaa ttaagagttt atccaagttc attttagctg agaatacgct tgaaaataat
     3721 tttgatgaat tttcctgaat taaaatttat taacttgttg agtgatataa ttgtaatgta
     3781 ttaccaaata gttagcactt agattagtga tggaaaggaa tagtgagaac aagatgactg
     3841 gtagaaatcc caaagtattt atatcatatt catgggagaa taaggaaaat aaagaatggg
     3901 ctagagaact agcagacaaa ttacttgaaa acggtattga tgcatatata gatcagtatg
     3961 atttaagttt aggtgataga ttacctcagt ttatggagca gcaaatatcc gattcagatt
     4021 atgttctaat tatatgtaca ccagagtata aaaaaaaggc aaataatcgt acaagtggtg
     4081 ttgggtatga aggtcatatt atctcagcag aattgctttc tggaaaaaat gaacgtaaat
     4141 ttatcccaat aattaaagaa ggacgtgcaa gcgaagtact tcctactttt cttgctggaa
     4201 agcttggaat tgatttatca gatagccagc attatgatga aaacttagat gatttaatta
     4261 caactatatt tggtgttaag agaaaaccag cattaggtga tatacctagg catattaaag
     4321 gcagtccttt actacctata gaacataatg aaaataaaga tattgaaatc ttaggaatta
     4381 tcactgataa agtaactgta cctaaaatgg atggctcaag gggttctgca ttatataaaa
     4441 ttccatttag attaaataga gtaccaagtc agctttggaa agagttgttt gttagaaatt
     4501 ggaattcacc accacgatat acaacaatgc atagaccagg aattgcttct gttgtagacg
     4561 atgaaatttt acttaatgga acaactattg aggaagtccg ggaatatcat cgggaaactt
     4621 taaaactttg cattgaaaaa agtaatactc aagaaaaaaa aatattgcta gaggaggaac
     4681 aaaaaaaaga gagagagaat gagcgagtca gatcacataa agaaagtgtt cagaaattag
     4741 cagatgaatt agagttctag ttgatagtca gttctttctt aattcgatac acagtttgtc
     4801 tagttacatt atagtctgtt gctatttttg aaatagcaac accattctcc tggtcctgaa
     4861 cgatgcttct ataaacaagg cgacgttgag gatcttttgt gttcgcactg tataacttag
     4921 gacgtccttt atagacgccg tttgctttag caatctttat cccttgtgcc tggcgtcgtt
     4981 ttgattcagt acgctcttgt tcagcaatca tggccaagat ttgaatgatt aggtctttga
     5041 taaatttatc caggagagga tttccgattg cttcagccat gatcggtaaa ttagtgataa
     5101 taagtcgcac atttttcttc tttagataat ttactgaatc gataatctcg tcataattac
     5161 gtccaagtcg atcgatagcc tctacaataa aaatatcttg gtctctaaca aaatctagaa
     5221 cttcctgaaa aatcggtcga tgagtaatag tcgctccaga ttgtttctcg atgaaaattt
     5281 tttcagcacc aaattttctc atttcctcga tttgacgttg ttcattttga tcgatcgat
//
LOCUS       DACEUZ010000140         5221 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.140, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000140 DACEUZ010000000
VERSION     DACEUZ010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5221
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.140"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            <1..1010
                     /locus_tag="IXG44_002324"
     CDS             <1..1010
                     /locus_tag="IXG44_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1659020.1"
                     /translation="QKNYKTFIIAPYNYATIQENNKEFFIGQKVYPNFEDYVSQPAGA
                     GICVDLNYLKYYNPISFEESTDLNLINSDPLTTYILVPKKYKEYAKMIEKNYLEDIQF
                     RLEDSPPQAPYKNPNLKNLKIKLIFVNNNQKYFSFNTLYGKAKDNYTITDPIVRVINP
                     EITESLFWGNILTSDGGLFFDQKHTSNQNFFNQINPYIKEFNLENIINFSYSVFQETG
                     ELLSQKKVNKLNAYLQYLLILGLFIIINFQMIFVSFTINSKKIALKKIFGYSPISQVL
                     DLCLLPFTIELFATMCFQLIFSKNTYLYFIIFMIFLINLITSIIIYKKNSFKYFKENI
                     L"
     gene            1007..1627
                     /locus_tag="IXG44_002325"
     CDS             1007..1627
                     /locus_tag="IXG44_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1659021.1"
                     /translation="MILSVENFTLSINKKKIKQNWNFSISEKDFIIVTGKSGSGKTTL
                     LNNLSLIDKQYTGNLFYFEKKVSKHDIQRLRRNHISYLFQNYALLENQTVQYNFELAK
                     KFNSNISNDHIYNLLTDFSLPKDILKEKIFLLSGGEQQRIALIRNMLKPFDILFADEP
                     TGNLDKENSTFIMNFLKNLTEKEKKAVVLVTHDLNVLNYGTNIIEL"
     gene            2320..2700
                     /locus_tag="IXG44_002326"
     CDS             2320..2700
                     /locus_tag="IXG44_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAQ1659022.1"
                     /translation="MSEQNQNLASNSSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVASG
                     DAQRLSENLKIMQKELQKIWQQLR"
     gene            2682..3596
                     /locus_tag="IXG44_002327"
     CDS             2682..3596
                     /locus_tag="IXG44_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAQ1659023.1"
                     /translation="MATIKVSRTTSCSRAINYAEPRATIKTGINCDVDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQTAIYTHTDKDHIHN
                     HIVINSVNLETGKKFQAHGQAFLNKCYDLNDEICLAHNLPITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRIAIDQTMKNKKTRTYDQFCNSLKTLGIHCFNRGKNFTYELIHKKK
                     KVRSNRLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTLTP
                     EQPEIRPARRNHEQHVERGTDHQQELSL"
     gene            3836..4525
                     /locus_tag="IXG44_002328"
     CDS             3836..4525
                     /locus_tag="IXG44_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1659024.1"
                     /translation="MAFYLPYLLIFVSISGSIWLIYKIFQTRYSLKGSKIRFKRFFFL
                     GCIFSLIIVSSGLFGVLEGNKRVSRSILLGNVTQKYESARDKKKKEQALAQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYVKDTKI
                     EEACSDLFGDTNPWFASEEEKKEKQQSVTYERYEILLQQATNMQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(4646..4732)
                     /locus_tag="IXG44_002329"
     CDS             complement(4646..4732)
                     /locus_tag="IXG44_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAQ1659025.1"
                     /translation="MFELFICPLLVGVCVSLFSYWLNNRNKK"
BASE COUNT         1980 a          843 c          877 g         1521 t
ORIGIN      
        1 tacagaagaa ttataaaact tttattatcg cgccatacaa ttacgcaact atccaagaga
       61 ataataaaga attttttatt ggacaaaaag tttatccaaa ttttgaagac tatgttagtc
      121 aacctgctgg tgcaggaata tgtgttgatt taaactatct aaaatattac aatcctattt
      181 cttttgaaga atctactgac ttaaatttaa taaactctga tccactaact acttacattc
      241 tagtacctaa aaaatataaa gaatatgcaa aaatgattga aaaaaattat ttagaagaca
      301 tacagtttag acttgaggac tctcctcctc aagctcctta taaaaatcct aatttgaaaa
      361 atttaaaaat aaaactaata tttgttaata ataaccaaaa gtattttagt tttaatacct
      421 tatatggaaa ggcaaaagat aactatacaa taactgatcc catagttaga gttattaatc
      481 cagaaataac agaatcatta ttttggggaa atatattgac tagtgatggt ggattatttt
      541 ttgatcagaa acatacttca aaccaaaact tttttaatca aattaatccg tatataaaag
      601 aatttaacct agaaaatatc ataaattttt cttattctgt ttttcaagaa actggtgaat
      661 tattatctca aaaaaaagta aataaattaa atgcatatct acaatatctt ttaatattag
      721 gattatttat aataattaat tttcaaatga tttttgttag ttttactata aattcaaaaa
      781 aaatagcttt aaaaaaaata tttggatatt caccaataag tcaagtttta gatttatgtt
      841 tactcccttt tacaatagag ttatttgcta ctatgtgttt tcaattgata ttttcaaaaa
      901 acacttactt gtattttata atttttatga tctttttaat aaatctaatt acttctatta
      961 ttatttataa aaaaaatagt tttaaatatt ttaaggagaa tattttatga tattatcagt
     1021 tgagaatttc accctgtcaa ttaataaaaa aaagatcaaa caaaattgga acttttcaat
     1081 atcagaaaaa gattttatta tagtaacagg aaaaagtggt agtggaaaaa ctactctgtt
     1141 aaataattta agcctaatag ataaacaata taccggtaac ttattctatt ttgaaaagaa
     1201 agtttctaaa catgatatac aacgtctacg aagaaatcat attagctatt tatttcaaaa
     1261 ctatgctctt cttgaaaatc aaacagtaca atacaatttc gaattagcaa aaaagtttaa
     1321 cagtaatatt tccaatgatc atatttataa tttattaaca gatttttctt tgccaaaaga
     1381 tatcttaaaa gaaaaaatct ttctcttaag cggtggagaa caacaaagaa tagcattaat
     1441 tagaaatatg ttaaaaccat ttgatatatt atttgcagat gaacctacag gaaatttaga
     1501 taaagaaaac tcaactttta taatgaactt tctgaaaaat ttaacagaaa aagaaaaaaa
     1561 agccgttgta ctagtaacac atgacttaaa tgttctaaac tatggaacta atattataga
     1621 attataattt ttacccgtga tgctagctga tttaaaacta gcaccacggg tatttatttt
     1681 atattccatc tctcctatcc caaaatttaa ataatcactt aataaaaagg aggcacccta
     1741 gtgattttat tgaaaaaaga gtatgaaaaa ttttatgata gacttattta ttctttgcaa
     1801 ttgccaaaaa aattaaaccg tcaattacaa aagacttatg aaactaaatc aactgaagaa
     1861 tgggagagtt attgctagat ttttggtaga agtaacaaaa agtggacata ttatcataat
     1921 gactcgccga aagtctgata aaaaattggc taaaaatatt gctgaatgtt taataaaact
     1981 atcagtttta gctaaatagt tagtagttta cacaaaaaag catttatgtt ttttgttgca
     2041 aagtaacaat ttcatcttcg gtgaaataag cgaaacttta caaaaagtgg ctagcatttg
     2101 ctagcttttt ttgtattgtg gagcgtggct tttgaggggg attcaagggg gagcttgcgc
     2161 ccctttgaca agcggttatt tgcactaaat acgtaggtat ttggctagca aatatagagc
     2221 ttgccaaacc cttttagtga gctttactca ttgtaccttt ttaggtacaa tggtattgcg
     2281 aacgatacgt ccgtaggacg tactgaaagg gtgggactta tgagcgaaca aaatcaaaat
     2341 ttggctagca attcttctaa aaaatatacc tatcgatccg agccaaaaca aatcagtttt
     2401 cgagtgagcg aatccgaatt tgcaaagctg aagcaatcgg ctgaagcttt gcaaatgagt
     2461 gtgccggctt tcgtgaaagc caaggcacaa aacgcgcgtc tggtgacacc gaaagtggcg
     2521 ccagacattg cccaggcaat cgcgcgcgat ttagccaaag ctggagggaa tatcaatcaa
     2581 attgctaagt ggtgcaatac acaccaacat gacgttgctt ctggtgacgc tcaacgttta
     2641 tccgaaaacc taaaaatcat gcaaaaggag ctacaaaaga tatggcaaca attaaggtaa
     2701 gccgaaccac ttcttgtagt cgtgcaatta attacgcaga accacgagca accattaaaa
     2761 caggaataaa ctgtgacgtc gattatgcca aaagtgaaat gaaacaaatt cgcatgctgt
     2821 atggaaaaga cgaccgtgta caggcacact tattgattca atcttttcga ccaggagaaa
     2881 ttaccgctga aaaagctaat caactagggg cagagtatgc aaaaaaaata gcccccgatc
     2941 accaaacagc tatttacaca catacagaca aagaccatat tcataatcac attgtcatta
     3001 actccgttaa tctggagact ggaaaaaaat ttcaagctca tggtcaagca tttctaaaca
     3061 aatgttatga cctaaatgac gagatttgtc ttgcacataa cttaccaatc actgagcgag
     3121 gaaagaaacc agaaaaacga accatgtctg aaataaaatt aaaagaaaaa aatgaacctg
     3181 tttggaaaga cgaaattcgg attgcgatcg atcaaacaat gaaaaacaaa aaaaccagaa
     3241 cctatgacca attttgtaat tccctcaaaa cattggggat tcattgcttc aatcgcggga
     3301 aaaatttcac ttacgaattg atccacaaaa agaaaaaagt acgatccaac agactaggaa
     3361 aggactacga aaaggagacg attttacatg agctggacag acgagaacga acctataacc
     3421 aacagcgaac caacgagcta cgaagagctc gagctagcat tcgcgaaact gcagaagaag
     3481 tacgaagaga acaacgaact cttacgcctg aacaaccgga aattagaccg gctcgaagaa
     3541 accatgaaca acacgttgaa agaggaacag accaccaaca agaattatcg ctctaaaacg
     3601 ttaaaagaca tagaaaacga aataaagaaa caacgtgctt ttctcgttcc ttatacgcaa
     3661 gaaaggatac aaactacgat tgatttacaa gttaaaaaac tccatcaaga gctattagac
     3721 cagcgaaata aggaacagaa aaaggtgctt attcaacgat cattggattt tcttgccccc
     3781 ttatgttgct tagtcctgac actgttcgca ttttggttag tatggggagg ctaaaatggc
     3841 attttattta ccctatctac tcatttttgt tagtatttca ggaagtattt ggctcatcta
     3901 taaaattttt caaacaagat attcattaaa aggctctaaa attcgattta agcgattttt
     3961 ctttcttggc tgtatatttt ccttgataat cgtttcaagc ggcttattcg gcgttttaga
     4021 gggaaataag cgcgtttcta gaagtatttt attaggaaat gtcactcaaa agtatgaatc
     4081 agcgcgtgat aaaaagaaaa aagagcaagc attagcacaa aaaatggaag agtttaccac
     4141 ttgttatgag gaaatgaacg atatttttgt gaatcaagaa aaacgtttga cggataaaaa
     4201 catggaaaaa ctcactcgtc tttatcaaaa cttgccagaa aaacagcaaa aagaggttca
     4261 agacaactat gagcgaacca aaaaagacgt gcaatatgta aaagatacaa agatagaaga
     4321 agcctgtagt gatctatttg gagacacaaa cccttggttt gccagtgaag aagaaaaaaa
     4381 agaaaaacaa caatctgtca catatgaacg ctatgaaatc ttacttcaac aagcaactaa
     4441 catgcaaaat ccgacaaaaa aagaaaccgc cttaaactat ctaaaaagtg tcaaggaatg
     4501 gttagatcag caacaacaaa attgaatttg atttaaaatc atgttaaact gaatgtgcaa
     4561 atgatcaaac attagataca cgaaagcccc agagaggtcg gattctctgg ggcttttttt
     4621 caggttgatg tcgcctttat tgattttact tcttgtttcg gttattcaac caataactga
     4681 ataaagagac acaaactcct acaagaaggg ggcaaatgaa cagttcaaac attagataca
     4741 cctccttcca gtcgcaagac tgtcggatag gcgacaagtt attatatctt aaaaaattaa
     4801 atttgtgtgg tgtttttaag tatcacttgt tttagggttg ttagggcttg ccctgaccgc
     4861 ctgtaaggcg cttctcgact agattaaggg aaatttaatc aagaattgtc ttagaacggc
     4921 ttagacaagc tttcagagtc tcacacctcc tctttgaaca acggttttag caaaggagcc
     4981 ggaggcgact gcgcagtata atatgagagc atagcgatca caccttttta gcgaccgtag
     5041 ggagcaagtt ttccagttta gaaaaattcg cgaacctttt tggaggaaaa agtaggagtt
     5101 ttttaggcgg gaaattcaat gtgctcttaa gatatactgt tagatatact gttagatata
     5161 ctgttaataa taactgttag ggattagcaa ccttatggga gagtaagtat tgacataggt
     5221 a
//
LOCUS       DACEUZ010000141         5179 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.141, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000141 DACEUZ010000000
VERSION     DACEUZ010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5179
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.141"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            4..360
                     /locus_tag="IXG44_002330"
     CDS             4..360
                     /locus_tag="IXG44_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1659026.1"
                     /translation="MIKVVIADRMGKGQNVAKGVEAAGGKAVVVPGMGADMRLGDVMQ
                     QEHADMGISFCGSGGAGALTAATKYGYPERHGMRSIEEGITAIKDGKTVLGFGFMDQE
                     ELGKRLTEEFIKKHGK"
     gene            376..726
                     /locus_tag="IXG44_002331"
     CDS             376..726
                     /locus_tag="IXG44_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4312 family protein"
                     /protein_id="HAQ1659027.1"
                     /translation="MSVQKTIQQKVIVSGKGETKQHAFASALAEIQNKIMEQSNVLLR
                     IEPVNVDILKAVENKYTERFLFFFFPRQRISYEVELSVTVAVTEIKLADISFSQQMTE
                     DPNGIAIPFVTKKI"
     gene            755..1534
                     /locus_tag="IXG44_002332"
     CDS             755..1534
                     /locus_tag="IXG44_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4311 domain-containing protein"
                     /protein_id="HAQ1659028.1"
                     /translation="MDFVIILVKSVIIGGLLGFAASVGAARMFHAPNTQGLGAFRTLG
                     EMNACMGDPASHFSFGLGFFFNAWASSVGAGAFTQDVTHRIIPNWAAAALLAKNKDVT
                     QTMHDPKKMGIIGAVIGAVVVAFLNTTAAAIPEALQVTAVSVLVPAATILINTVMPVI
                     FWLAALDAGKRTGFWGTLFGGIAQLIMGNAVPGVVLGILIGKGVDESGWNRVTKSMMI
                     AIVLLFIFSGFFRGFDMEMIKSFQLQVPNWLDNLHNIFSVK"
     gene            1545..2222
                     /locus_tag="IXG44_002333"
     CDS             1545..2222
                     /locus_tag="IXG44_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4310 family protein"
                     /protein_id="HAQ1659029.1"
                     /translation="METTEEQAKVLEAKRQKSFWFADWSFPLIVGIMSAAVFAGTHMY
                     VMYGVGAFNEVSIVAMLKAGLDGGSYGAAAAFGASFLFARILEGSLVGILDLGGSILT
                     GIGIGVPAILLSMKIAAPIDNFALSLLTGLVLGLIVGGIIVLIRKFTINQSNSTFGAD
                     VMMGAGNSSGRFLGPLIILSACAASIPIGIGATLGALGFYAWKKPIAGGAILGAMILG
                     AIFPAVN"
     gene            2241..3347
                     /locus_tag="IXG44_002334"
     CDS             2241..3347
                     /locus_tag="IXG44_002334"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HAQ1659030.1"
                     /translation="MFDTLIKNGRLVDGSKIEVAIEKGMIKAVAAEINQPAQEVVDLE
                     GKYYLSAGWIDDHVHCYEKMNLYYDYPDQIGVEKGVTTVIDAGTTGAENIREFYQLTK
                     SVKTNVYALMNISKWGIVKQDELADLTKIQEDLVSTALAELPEFIVGIKARMSKTVVG
                     ENGIKPLELAKKIQTENNDLPLMVHIGSAPPELEEVLSRMERGDVLTHCFNGKPNGIL
                     DPKTDQIKNFVWDAYNNGVVFDIGHGTDSFNFHVAETALKAGMKATSISTDIYIRNRT
                     NGPVFDLATTLEKLRVVGYSWEEIIEKVTAVPARNFHLKNKGQLAVNYDADLTIFEIV
                     ENEKELKDSNGFTRIAKEQILPIKTIIGGVIYDN"
     gene            3337..4428
                     /locus_tag="IXG44_002335"
     CDS             3337..4428
                     /locus_tag="IXG44_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgaE family pyridoxal phosphate-dependent
                     ammonia lyase"
                     /protein_id="HAQ1659031.1"
                     /translation="MTISYGKFDLKEVINASGKMTILGVSKVSESVLAAQRFGGEHFF
                     EMSDLAIKTGAYLSQLLKVEDAQIVSSASAGIAQSVAALIGQGDMYHVYHPYTEKIAR
                     REIIIPKGHNVDYGTPVEVMVAQGGGKVIEAGYANMCTPEHVSMMITEKTAALLYIKS
                     HHAVQKSMLTVAEMVEVAKAHRLPLIVDAAAEEDLFKYSEMGVDLVIYSGAKAIEGPS
                     AGLVIGKKKYIQWIRLQSKGIGRAMKIGKDNILGFTQALEDYLKNGSESGDSMKQRLA
                     PFVNAINQLPDLSAKIVQDGAGRDIYRASVTINGDKSAKEVIQELKARNPAVYTREYQ
                     ANNGIIEFDIRSVSQNEMEKIIKRLREIM"
     gene            4441..>5179
                     /locus_tag="IXG44_002336"
     CDS             4441..>5179
                     /locus_tag="IXG44_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KDGP aldolase family protein"
                     /protein_id="HAQ1659032.1"
                     /translation="MSVIPNYYKNRVCLNVLAHSVENAKACYEAAEGHVVLGVLSKNY
                     ASDEAAIEDMKRYQDATNNALSVGLGAGDPNQSQMVSRLSGVLQPQHVNQVFTGVGTS
                     RALLGQNDTVINGLVSPTGKIGYVNIATGPLSSQAPAAEVPIETAIQMLQDMGGTSIK
                     YFPMKGLAHREEFKAVAEACAKYEFYLEPTGGIDLSNFEEILKIAIDAGVKKVIPHVY
                     SSIIDKETGDTRIGDVKILLSMVKKIIH"
BASE COUNT         1648 a          856 c         1243 g         1432 t
ORIGIN      
        1 atcatgatta aagtagttat tgcagaccgt atgggtaaag gacaaaatgt ggccaaaggg
       61 gtagaagcag ctggtggaaa agcagttgtc gtaccaggaa tgggggcaga tatgcgtttg
      121 ggcgatgtga tgcaacaaga gcatgcagat atgggtatct ctttttgtgg tagtggtggt
      181 gcaggtgcat taacagcagc aacaaaatat ggctatccag aacgacatgg tatgcgttcg
      241 attgaagaag ggatcacagc tatcaaggat ggcaaaactg ttctaggttt cggctttatg
      301 gatcaagagg aattagggaa acgtctgaca gaagaattta tcaaaaagca tggaaaatag
      361 aaagagggac atgaaatgag cgttcaaaag acaattcagc aaaaagttat cgtctccggt
      421 aaaggggaaa caaagcaaca tgcttttgca tcagcgttag cagaaattca aaacaaaata
      481 atggaacaat caaacgtatt attgcgaatc gaaccagtca atgtggatat tttaaaggcg
      541 gtcgaaaaca aatacacaga acgctttcta tttttcttct tcccacggca acgtattagc
      601 tatgaagtcg aactctctgt tactgtggct gtaacagaaa taaaattggc agacatatcg
      661 ttcagccagc aaatgactga agatccaaat gggattgcca ttccatttgt aactaaaaaa
      721 atctaatttt gaatcataag ggggaaagaa taacgtggat ttcgtaatca ttctagtcaa
      781 atcggtcatt atcgggggat tacttggttt tgcagcatct gtcggggcag ctcgaatgtt
      841 tcatgcacct aatacacaag gtttaggagc ttttagaaca ttaggcgaaa tgaatgcctg
      901 catgggtgat ccagcttctc atttttcatt tggtttaggc tttttcttta atgcatgggc
      961 ttcttcagtt ggtgctggtg cattcacaca agatgtgacg catcgaatca ttcccaattg
     1021 ggcagcggca gcacttctag caaaaaacaa agatgtcacc cagaccatgc atgatccgaa
     1081 aaaaatgggg atcattggag cggttatcgg ggcagtggtg gtagccttct taaacacaac
     1141 agctgcagca attccagagg cgttacaagt gacggcggtt tccgttttgg taccagcagc
     1201 gacgattctg atcaatacag taatgccagt tattttttgg ttggcagctt tagacgcagg
     1261 gaaacgaact ggtttttggg gcactctttt tggtggtatc gcgcaattga ttatgggaaa
     1321 tgcggtacct ggtgttgttc taggaatctt gattggtaaa ggcgtcgatg agagcggttg
     1381 gaatcgcgtc acgaagagca tgatgatcgc tattgtttta ttatttattt tcagtggatt
     1441 tttccgtgga tttgatatgg aaatgatcaa gagcttccag cttcaagtgc ctaattggct
     1501 tgataacttg cacaacatct ttagtgtgaa ataggaggag aatcatggag acaacagaag
     1561 aacaagcaaa agtattagaa gcaaagcggc aaaagagctt ttggtttgca gactggtctt
     1621 ttccgctaat cgtaggaatt atgtccgctg ctgtatttgc aggaacacac atgtatgtga
     1681 tgtatggtgt gggtgcattc aatgaagttt cgatcgtggc gatgttaaaa gccgggctgg
     1741 atggcggatc ctatggcgca gcggcagcgt ttggtgctag tttcttattt gccagaattt
     1801 tagaaggctc tttagttggg atcttggact taggcggttc gatcctaact gggatcggaa
     1861 ttggggtacc agcaattctc ttaagtatga aaatagcggc accaatcgat aattttgcac
     1921 tctctttatt gactggtttg gttctaggtt tgattgttgg tggcatcatt gtcttgattc
     1981 gtaaatttac gatcaatcaa tcaaattcca cttttggtgc tgacgtcatg atgggggcag
     2041 gaaattcatc tggacgtttt ttgggtccat taattatatt aagcgcgtgt gcagcatcta
     2101 ttccaatcgg cattggcgct acattaggag cactaggatt ctatgcttgg aaaaaaccaa
     2161 ttgctggagg tgctatttta ggagcaatga ttttaggagc aatcttcccg gcagtaaatt
     2221 aatagaaaga gggtcattaa atgtttgata ctttgattaa aaatggaagg ttagttgatg
     2281 gatctaaaat agaggtcgct atagaaaaag gcatgattaa agcagttgca gcagaaataa
     2341 atcagccggc gcaagaagta gttgacttgg agggaaagta ttatttatcc gctgggtgga
     2401 tcgatgatca tgtccactgc tatgaaaaga tgaatcttta ttatgactat cctgatcaga
     2461 taggggtcga aaaaggtgtg acgacggtca ttgatgctgg aactacggga gcagaaaaca
     2521 tccgtgaatt ttatcaattg acgaaatcgg tcaagacaaa tgtgtatgcg ctaatgaata
     2581 tctctaaatg gggcattgtc aaacaagatg aacttgctga tctaacaaag attcaagaag
     2641 atctagtaag tactgcgtta gcagaactgc cggaatttat tgtcgggatt aaagcgagaa
     2701 tgagtaagac agttgttggt gaaaatggca ttaagccatt agaattagct aaaaaaatcc
     2761 agacagaaaa caatgatctg ccattgatgg ttcatatcgg ttctgcacct cctgagttag
     2821 aagaagtatt gtcaagaatg gaaagaggcg atgtattgac ccattgtttt aatggaaaac
     2881 caaacggtat tttagatcca aagacagatc aaattaaaaa ctttgtctgg gatgcttaca
     2941 ataatggtgt cgtctttgat attggacatg ggacggatag ctttaatttc catgtggcag
     3001 aaaccgcact aaaagcagga atgaaagcta cctctattag tacggatatc tacatccgca
     3061 atcgtacaaa tggcccagtt tttgatctag caactacttt agaaaaattg agggtagtcg
     3121 gctattcttg ggaagaaatc attgaaaaag ttacagcagt tcctgcacgg aattttcatt
     3181 tgaagaataa gggccagtta gctgtcaatt atgatgcaga tttgacgata tttgagattg
     3241 ttgaaaatga aaaggaattg aaagattcca acggatttac acgtatagct aaagaacaaa
     3301 ttttaccgat taaaacaatt attggaggcg tcatatatga caattagcta tggaaagttt
     3361 gatttaaaag aagtcattaa tgcatcggga aaaatgacga tcctaggggt ttctaaggta
     3421 tctgaaagtg tcctagcagc acaacgattt ggaggcgagc atttttttga gatgagtgat
     3481 cttgccatca aaaccggcgc ttatctttct caattactga aagtggaaga tgcgcaaatc
     3541 gtttcttctg cttcagcagg gattgctcaa tctgttgctg ctttaatcgg acagggcgac
     3601 atgtaccatg tctatcatcc ctatacagaa aaaatcgcta gacgggaaat catcatacct
     3661 aagggacata atgtagatta tgggacaccg gttgaagtaa tggttgctca aggtggagga
     3721 aaagtcatag aagctggcta tgcaaatatg tgtacaccgg aacatgtttc catgatgatt
     3781 acggaaaaaa ctgctgcgct tctatatatt aaaagtcatc atgcagttca gaaaagtatg
     3841 ctgacggtgg cagaaatggt agaagttgcc aaagctcatc gtttgccatt gatcgttgat
     3901 gcagctgcgg aagaagattt attcaaatat agcgaaatgg gcgttgactt agtgatttac
     3961 tctggggcaa aagcaatcga aggtccgagt gccggactgg tcataggaaa aaagaagtat
     4021 attcaatgga ttcggctaca gtctaaaggt attggccgag caatgaaaat cggtaaagat
     4081 aatattttag gctttaccca ggcactagaa gattatttaa aaaatgggag cgaatccggt
     4141 gactccatga agcaacgatt agcgcctttt gttaacgcaa tcaatcaact tcctgacctt
     4201 tcagcgaaaa tagttcaaga tggtgccggc agagatattt atcgagcaag tgtaacgatt
     4261 aatggcgata aaagtgcgaa agaagtcatt caggaattaa aagcacgaaa tccagcagtc
     4321 tacacccgag aatatcaagc aaataacgga atcattgagt tcgacattcg ttccgtcagc
     4381 caaaacgaaa tggaaaaaat tataaaaaga ttgagagaaa tcatgtagga ggcttttaaa
     4441 ttgtcagtta tcccaaatta ttataaaaat cgtgtatgtt taaatgtatt agctcattca
     4501 gttgaaaatg caaaagcatg ctatgaagca gcggaagggc atgtcgtact aggtgtgcta
     4561 tcgaaaaatt atgctagcga tgaagcagca atcgaagata tgaaaaggta ccaagacgca
     4621 accaataatg cgttgtcagt tggattaggc gctggagatc caaaccaaag tcagatggtt
     4681 tcgcgtttat ctggggtttt acaaccacaa catgtcaatc aagtatttac tggcgtgggg
     4741 acttctcgtg ctttattggg ccaaaatgat acagtcatca atggattggt ttcaccaaca
     4801 ggtaagatcg gctatgtcaa tattgctaca ggtccgttaa gttcacaagc gccagcagct
     4861 gaagtgccta ttgaaactgc cattcaaatg ctgcaagaca tgggaggtac ttcaatcaag
     4921 tatttcccga tgaaaggctt agcacatagg gaagaattta aagcagttgc ggaagcttgc
     4981 gcaaaatatg aattctattt agagccaaca ggaggaattg atctttcaaa ctttgaagaa
     5041 attctgaaaa ttgcaatcga tgcaggtgta aaaaaagtga ttcctcatgt ttatagttca
     5101 attatagata aagaaacagg agatacgcgt ataggagacg taaagatact attaagcatg
     5161 gtaaaaaaaa ttattcact
//
LOCUS       DACEUZ010000142         5119 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.142, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000142 DACEUZ010000000
VERSION     DACEUZ010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5119
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.142"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(332..817)
                     /gene="vanZ"
                     /locus_tag="IXG44_002337"
     CDS             complement(332..817)
                     /gene="vanZ"
                     /locus_tag="IXG44_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HAQ1659033.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(970..1881)
                     /gene="vanY"
                     /locus_tag="IXG44_002338"
     CDS             complement(970..1881)
                     /gene="vanY"
                     /locus_tag="IXG44_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HAQ1659034.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYELWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            complement(2309..2917)
                     /locus_tag="IXG44_002339"
     CDS             complement(2309..2917)
                     /locus_tag="IXG44_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HAQ1659035.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
     gene            complement(2923..3954)
                     /gene="vanA"
                     /locus_tag="IXG44_002340"
     CDS             complement(2923..3954)
                     /gene="vanA"
                     /locus_tag="IXG44_002340"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HAQ1659036.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALAVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            complement(3947..4915)
                     /locus_tag="IXG44_002341"
     CDS             complement(3947..4915)
                     /locus_tag="IXG44_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HAQ1659037.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
BASE COUNT         1469 a         1142 c          878 g         1630 t
ORIGIN      
        1 tctttagcga atgaagacat cctttccgct attcgtgttt tttaaagtta tcagaaaatc
       61 tatctatgaa atcttagttt gggggtaccg tcaggaaaat gcggatttac aacgttaagg
      121 aaatttatta aagagaccaa ttcctcagca ctaaaattga taattgggct ttctttgtta
      181 ctatagtgtt aggtaagctt tttactttat caccggtttt cccctcactt cacacctacg
      241 ggcgagtttc cccacatccc gttttccatc taatccaatt cattcaatat ctttatgtgt
      301 atgaaagatc agattgcttg atttagacat cttacacgta atttattctt aaatgggtac
      361 ggtaaacgag caataatacg agcaaaagta tacctacaaa aataataact ctgtctaatt
      421 ttttttgatt catatgctta ttgcttaaac catataattt cagtccaaga aagcctccaa
      481 cagtatttgt aattacatct gttatgtctg tcgctccaat agcgaagata aattgaatta
      541 tttcaaaagt aagacttaaa accagtacaa aagcaaactt aggtaaaaat ccgatttctt
      601 taaaattgac attcaaaagc aagccaaatg gaataaagat tataacatta tctatcatct
      661 ctctgaaatt cccagtagca gtaaatggag tcaagttaag acttctttga tgataattaa
      721 atactgataa aatattgtat tgtaatttga ataacactaa ccagattagt gtcactaaat
      781 ataaagctag caatcctcta gataatattt ttcccaatat aatttcctct aattcttttg
      841 tttaagttgt atcattagta tagcaatgtc ctagttaacg tgggttttaa attaaaaagc
      901 taacgttacc ccaaaagtgc tagtacaata atgctgttca ttaatttcgt tggtttcatt
      961 ccgccatcct tacctccttg aattagtatg tgttgatccg ggaaacactg tcacaattac
     1021 accgtctata ttgtttcctg atatctcata acgaagatta gtcggcacat gaatggtggt
     1081 atttttagta acaggataat aaaagatctc atatttttcc ccatttacac taacagaaat
     1141 ggttttttct tcttttaggt aatccatata ttcctcgaga acgaaattct tttctttcat
     1201 aatcgcacta tgtggtaaac caacatagcg aatatgccat agttcatatt gaattcctgt
     1261 taactctgtt ttgtcctctg gataacgtaa aatgaacccg tatttccaag cattttcttc
     1321 tatccacttt ccttcagggg ctcgttccat tttcgtcaag cttgatccta catctagtga
     1381 taaacctgaa ttatgctcac tataacctgc tggtaaggca tactcagccc ccatttcttg
     1441 gtaaagcaca ctttgctcat caaagtctcg atagccacta ttaataataa aatgactaac
     1501 gccacccttt acagcatcat tgaccatctc tgaaaatttt tgtgctattt cttttgacat
     1561 ataaatatta ctatcaagca acccgtatcc atttattaat tcgtcatgtt tagataaatt
     1621 cacgatatct gacttcacac tttcttggcg aacaggatat ttactattga ttaatagcag
     1681 atttccttga taaacctgtt cttctgtaat tgttttctct tgggtatttt cagaagtccc
     1741 actattttct aaatgttctt tgggattttg atcataattt tgaaattcga ctttttcctg
     1801 agaaaacagt gcttcattaa cgtagtcata acctaagtat attaagaata acaataacaa
     1861 taaaaaaaac aacttcttca ttttcagtct cctccttaac tatatagttg gaagtatgaa
     1921 atatttctag aatcttcccc ttaaacggca caatctcgtt tgagattgtg ccgttttgcc
     1981 ccgtaaatta cagtcaatta tttcgcccta ctggatttac tattatcacc aatgtagatg
     2041 aattttcata agtgataccc atgctgaatt taacgaggct gaactgcctg ttgacaatgg
     2101 gcggtgtgaa ataccacccc tctatgcgat cagtcttatt atcaggctat cttgccgcag
     2161 cgcacgcaga taatgaccgc aatatcgctc tgccttacat cttcaatact atctactata
     2221 tataaattcc ctaagaacca gttacatacg tcgggtttcc tgccgaaaga gttagcttat
     2281 atagtttgtc cgtgcaacgg ttaaaagttt atttaacggg gaaatcaaaa tagctattgg
     2341 ggtatggttc gtctcttaat acatagtgcc accattcgag gctatatgct tcaaacccac
     2401 tgttttccat gatggagcgc aaacgtctgc gattttgcgc ttcattgcat gatattccat
     2461 ttgccgcatg atgagagcgt tcatccataa aatcaaatcg gctccccatt ggtacaagct
     2521 cacccgtgtc taatcgataa agcgtaagat caatggcact gccgcggcta tggcttgatt
     2581 ttgaagccac gtatcctttt gaaatcatct cagttcggtc aatattggga taataacttt
     2641 cctttgtcag gttattttcc ggctgtgcag cccattgcat aaaacagttt acagcacgat
     2701 taggacggta accgtcccat agaagcaatc cgtacccttg ggtagcagcc agttcttttg
     2761 ccttcaaaag cgattcagcc aactcgtatg tccctacaat gcgatttact tcataaccgt
     2821 caaccggttt tccggtgaaa ttatcccaag tggcatattt agcgtcccaa cgaacaccgt
     2881 gtactatttc atctaaaaaa gtaaatccta tttccatgct tatcacccct ttaacgctaa
     2941 tacgatcaag cggtcaatca gttcgggaag tgcaatacct gcagcggcca tcatacgggg
     3001 ataacgactg tatgacgtga aaccgggcag agtattgact tcgttcagta caatgcggcc
     3061 gttatcttgt aaaaacatat ccacacgggc tagacctcta cagccgagcg ctttatatat
     3121 tttttttgcc gtttcctgta tccgtcctcg ctcctctgct gaaaggtctg cgggaacggt
     3181 tataactgcg ttttcagagc ctttttccgg ctcgacttcc tgatgaatac gaaagattcc
     3241 gtactgcagc ctgatttggt ccacctcgcc aacagctaac gcggcactgt ttcccaatac
     3301 cgcacaaccg acctcacagc ccgaaacagc ctgctcaatt aagattttgc tgtcatattg
     3361 tcttgccgat tcaattgcgt agtccaattc gtccgcgcta ttgacttttt tcacaccgaa
     3421 ggatgagcct gaacgcgccg gcttaacaaa aacaggatag gtaaacgtag ctgccaccgg
     3481 cctatcatct ttattaataa cccaaaaggc gggagtagct atcccagcat ttttcgcaac
     3541 gatgtatgtc aacgatttgt ccatacaaat tgctgagctt tgaatatcgc agcctacaaa
     3601 agggataccg gacaattcaa acagaccttg tatggatcca tcttcacctg acttgccatg
     3661 caaagctgaa aatgctacat caacatggtt gatttcatat tcatggttct ttttaacaag
     3721 taatccgtgc atttttttat ccggcgagag tacagctgaa tagcaattgt cgttttccca
     3781 ttccgcgcaa ggtttttcgc acattttcca tacaccagat ttcgtaattc caatgtataa
     3841 cggctcgtat ttttctttat taatgttagc ggctatctct attgcagatt ttaccgatac
     3901 gtcatgctcc tctgagcaac ccccaaacag tattgcaact tttattctat tcatgctcct
     3961 gtctcctttc aaaatccaaa cagtttttaa tggttttttc aacggtatca cgcaacgctt
     4021 gctcggtata ataggccgta tgcggtgtga ttatcacgtt aggcattctt tgaagtttaa
     4081 gtaaaaattg attatcaatt ggtttttggg tgcaatcaga gtagaaaaac tcttcctctc
     4141 cttccaatac atccaatgcg gcaccgccca gtttcccgtt ttctaatgct ttaaccaact
     4201 cataggtatc tacaagtgga ccgcgcccag tattgataag aaatgctcct tgcttcattc
     4261 tctgtatttg ttcgtggctg ataatatagt gcgtatccgt attgagcggc acatgaagcg
     4321 taacgatatc gctattttgc agcaactcat caaacggtac atagtttacc tctatacttc
     4381 ggctgcgact ataagccaac actttacatc caaatcctcg cagccgctca ataaccgctt
     4441 tgcctatctg gcccgttccc accacaccaa ctgtcatgtc gctgagtacc ttgccacggt
     4501 cgctgtccaa cctgaaatca tgtttttcca cagagcgcac aatcgatttt acgttgcgta
     4561 ctgccataag aattagcatc atagtataat cggcaacgct atccggcgag tacgccacat
     4621 tgtcgacagt gatgcccatt ctcttagcag cagttgtatc tatatgattg cagccgatgc
     4681 ttcgggtaga aatatatttc acaccggctc tcttcagcgc aagaagaata gaggcggaaa
     4741 tctctgattt atgtcccaca ctgatacatt gattgaaagg cgcggatttg gcgttggatt
     4801 ccgacacgtt ggcgttaatt atcgttgcca taacgccaaa gcgaggcgaa agagcatgga
     4861 atgcatctgc ctcatcctgc tcacatccat aaacagtaat gccgatgtta ttcataatta
     4921 agaccaaccc tttctgtgaa aggcaccaaa taaaacgggg atgccaatgg taatcttgtc
     4981 aaatctttct ctgaagaacg aaaacggctc gttcttacgg tattaagtct accgtgtaag
     5041 aacgagccgt ttcttaatat taaattgtta atttcctaag aaaaagtaaa gataaccttg
     5101 agattttcct aaaaaatta
//
LOCUS       DACEUZ010000143         4953 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.143, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000143 DACEUZ010000000
VERSION     DACEUZ010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4953
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.143"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(21..905)
                     /gene="rsmA"
                     /locus_tag="IXG44_002342"
     CDS             complement(21..905)
                     /gene="rsmA"
                     /locus_tag="IXG44_002342"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HAQ1659038.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            complement(898..1467)
                     /gene="rnmV"
                     /locus_tag="IXG44_002343"
     CDS             complement(898..1467)
                     /gene="rnmV"
                     /locus_tag="IXG44_002343"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HAQ1659039.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            complement(1460..2236)
                     /locus_tag="IXG44_002344"
     CDS             complement(1460..2236)
                     /locus_tag="IXG44_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAQ1659040.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            complement(2391..3713)
                     /locus_tag="IXG44_002345"
     CDS             complement(2391..3713)
                     /locus_tag="IXG44_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1659041.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            3888..4475
                     /locus_tag="IXG44_002346"
     CDS             3888..4475
                     /locus_tag="IXG44_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1659042.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
BASE COUNT         1408 a         1042 c          851 g         1652 t
ORIGIN      
        1 ttctgtcaca acctcttttt ttactgcata ttttctgaca tcgcattgct taatgcggcg
       61 aattcttgta atgacaaagt ctccccacga cgttttggat cgattccagc tgtttccaag
      121 ctttttgcta accatgcttt tgtctggtca tctttgccat acgaatgctg caagttattc
      181 caaagcgttt ttcttctttg ctgaaatgcc gcttttgtca atcggaaaaa tgctttttcg
      241 tctgtcactt cgactgccgg tatatctctt cttgtcagtt tcaaaatcgc tgaatctaca
      301 tttggctgag ggacaaatac tgtttttggt acgatgaatg ctaaactggc ttccatataa
      361 tattgaacag caatcgataa tgaaccataa gctttcgttc ctggttcagc agagatacga
      421 tcagctactt ctttttgcat catgacgact agttcatcca caaccaaatc tgattcaaga
      481 aaatgcatca tgatcggtgt cgtaatatag taaggtaaat tcgctacgac ttttaacgga
      541 agctcctcat gaaatgtttc tctaacagct gttgacaaat cggtctttaa aatatcttga
      601 tgtacaatag tcacattgtc atagggttgc atcgtatcag ccaatacagg gatcaaacga
      661 tcatcgatct caaatgctac gacttgtttt gcatgcttgg ctaattgttc cgtcaatgca
      721 ccgattcctg gtccaacttc gatcacgttc gtatgttcat ctatttgcgc agtttctacg
      781 attttcctta aaatatttgg ttcagtcaga aaattttgtc ctaaactttt tttaaatgaa
      841 aaaccatgtt tcaataaaat ctcttttgtt ctagagggtg tcgcaatttc acgatattca
      901 ctcatgttgt tcctccacta ttttcattgc ttgggcgagt tctttcggcg taatacgaaa
      961 catcttcaaa cgtttttcca attgtttgcc gttcgtgtaa ccgatgcgca actcgtcacc
     1021 tagtttttct cgtttttctt ttgcttgtgc cccagctatt aaaccatagt ctaaaaggtc
     1081 ttgtctagtg atttcagaaa cagccgtttg ggaaatgtct ggcgtcacga cctttttcaa
     1141 tgcttctaaa atagcagcat cacttgcatg ctcgacacca aggcttccct ttttctttgg
     1201 tttagcttgt gtgcgcggca aaaaagcatg ttttgcctga ggaacagctt ccataatgat
     1261 ttttcttatt ttttctcctg aaaaatcagg atcggtaaaa acgatcactc cacgagtctc
     1321 ttgggcatgt tcaatttgca tcaggatctc ttcattgatt gcagacccta ttgtttctat
     1381 cgtatctgca tcaactactt cccgcagcct tcttgtatcg tcttttcctt caacgactat
     1441 aatctcttgg atcttcatct tatccatgtt cgcttagacc aaataatcgg tgcgcatttg
     1501 tccatgtttg ggcagccact tcttctgtcg gaagattccg aagttcagca atcttttcta
     1561 ctgtataacg agtatagcct ggttcatttc gctttcctct aaaaggaact ggtgccagat
     1621 aaggggcatc tgtttccaca agtaatcgtt cgattggtac atttgctgca gcctcttgga
     1681 cgtctaatgc ttttttgaaa gtgacgacac cgctaaatga aatatgcatc cctaagtcaa
     1741 gaaatcgttt tgcccattca taatcaccac tgaaactatg catgattcca ccgatatctc
     1801 gaacatcttc ttcttttagg atttgatacg tgtcttcaag tgcttctcta gtatgaatac
     1861 tgattggtag gttcatttct tttgcgatag caatctgtct gcgaaatact ttatcttgga
     1921 cttctttcgg gtcttccatc caatgataat ccaagccaat ttcccctaat gctacaacct
     1981 ttggtaaagt caattgttct tgtaatttat tttcgatttc tttcgtataa ctgccagcct
     2041 ctgtcggatg ccagccgata atactgtata tatttggata gtcatggctt aattgcaaag
     2101 acttttcaat cgttggcgta tcgaagccca cgaccgccat tttcgttacc cccatttctt
     2161 gtgctcgctg aatcgtttct ggaatatctt cattgaactg ttcagcattt aaatgggtat
     2221 gagaatcaaa tatcatgttt atctctcctt ttcattctta ctttagcaca taagcactac
     2281 ttgcagaagt agcaaaaatc aaaatagaag aacttgtttt attgaaatag aaaaagcacg
     2341 aagatctaga ggacctagtt cgtacttttt ctgtctgttg ttgaaatatt ttactcttct
     2401 gagcgttttt tctttttttg ataatagact gggataccta tcaaggtaac acctataccg
     2461 atcaatgcta atttcgtttg cgtcatcatc gtcataccta aaatgaaaat agcaccgaga
     2521 atcgcaataa tcggaacgat tggataaagt ggtactttat atggacgtgg caattccggc
     2581 gctttttttc ttaaaataaa gacaccgata aaaatcagta aactgaatag ccacatcaca
     2641 aaaatcaaca tgtctgttaa aaagtcgaaa gaaccaaggc tcatcataat acaagctact
     2701 gctaactgga aaaccgcagg aacataagga accgtaaact tctttgaaag ctttgtcagc
     2761 tgtttactga aaggtagctc gtcttctaaa gccatcgcat aaggcacgcg gatcccagtt
     2821 agtgtataac cgttcaatgc accgtatacc gagatcaaga tcccaatagt gaccagcttt
     2881 ccaccgattc ctccaaaaat gacgtctgat gcttcagaag ctgcattcaa attaccggca
     2941 agatgatcaa tcggcaaagt tttcaaaaaa acaaaattga tcaatagata aacgactgtt
     3001 acaaaactca aaccaagaat aattgcttta ggcaaatctt tttccggccg tttcatttcc
     3061 cctgccattg caccgacacc taaccagccg tcataagcaa acaacgttgc taacaacgca
     3121 ctgctcaatc cttcgacaaa agtaacatct tttccagctt cgattggaaa taactggaca
     3181 gttccttgtc ctggggtcaa taatccccat atgacaatca cagcaattgg tattaattta
     3241 acaatcaacg tcactgattg tacagaagca ccgacttttg tccctaacaa attcaaccct
     3301 gtcacagaaa cagcggtgat cactgcaatc aacaaaagat aatcggtaga taattgtaat
     3361 aagttcgtca attgtgtcgc aaagataatc gataatgcag caatattagc aggaaagtaa
     3421 atcaagcttt gcgcccatcc taataaaaag ctgggcaatt ttccatacgc agcttcaata
     3481 tattttacag ctccaccagt ttccgggatt gctgtagcta attcagccac tgtcaatcca
     3541 gcacagatcg ttaagaaacc acccaaaagc caagctagca atgtcaaact tgtagactga
     3601 gtactagcag taacagcagc agttttgaag aagacgcctg caccgatcac ggttcccata
     3661 acggtagcta atgcgccaaa cagattgatt tctcgtttta gtttgccttt cattcccatc
     3721 ctctttcttc tttgaaattt ggtataattt tcagtataac tgagaatatt ttaatttacc
     3781 attctttttt ctccactttc agtgaaaaac tccctcttaa gacggatgta tttctttttt
     3841 atttttcgta tactaaacct accttttcca cgaaggagga acagtttatg gattttaagc
     3901 gttcttatcc gaaattagca gattatttat ccaaccgggt cgatttttcc cgtaaagaac
     3961 aacagcagtc ccattggcat tggttcacat taattttatt tggattaatg actgttgcaa
     4021 cgttatcaca aaaactaata ttgctgactt tgttcatcgc aatcgttgca ttgatcaaag
     4081 gtcctgtgat gatcttgtgg gggatgttgt attccttttt ggttgggctt tttccgccta
     4141 tcggtatttt gctttcagcg attttctttt tattgaatat cggaacattt acaaagaact
     4201 ggcggatgac cttagtcggg atctattttt atttctatcc ttttggagtg atggcactta
     4261 gtgaagtcat gcattgggat aatcattggt ttatagctgg ctccttgctt ttagggttga
     4321 tcttgttaca cgtgatgttg acaaagctct atcaacatta tggtatcgga cgtacgattt
     4381 tctggtatgt cttttcgatc ccatttgcac tgcttactgc tctcttacca agccgtttga
     4441 agacaaaaat aaaaggttac cataaaatca agtgatagga tttttacaaa ataaagaaaa
     4501 caatcatcga agtcaaagcc gatgattgtt tttttatcta tattttcaat cacacatata
     4561 agagcacaaa ctaattgaac ataaagtgat agaacttata attaacacat caacgctact
     4621 gttagatata aatatagatg taataaagtc attgcattca aactttatga atgtaaggac
     4681 aataaaaaag agtaccagat gaatcaatta aaatctgccc gaccctttat cattatgctt
     4741 tttttctgtt tttacgattc ctattagtat tattctaaat taacaaaaat cagcgaagct
     4801 cgtacttctt tttctaggat ttattttgga tagttctctg tttctgtatt atatgattgg
     4861 cttcaagtaa taagaaggaa tttacgaaaa ttgcttctca aatttcggaa aaagggggta
     4921 tgacacaact agtgtcatac cccctttgaa ttt
//
LOCUS       DACEUZ010000144         4927 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.144, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000144 DACEUZ010000000
VERSION     DACEUZ010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4927
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.144"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            83..970
                     /locus_tag="IXG44_002347"
     CDS             83..970
                     /locus_tag="IXG44_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ1659043.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
     gene            1110..2036
                     /gene="cysK"
                     /locus_tag="IXG44_002348"
     CDS             1110..2036
                     /gene="cysK"
                     /locus_tag="IXG44_002348"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HAQ1659044.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            2272..2718
                     /locus_tag="IXG44_002349"
     CDS             2272..2718
                     /locus_tag="IXG44_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAQ1659045.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2991..4343
                     /locus_tag="IXG44_002350"
     CDS             2991..4343
                     /locus_tag="IXG44_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase"
                     /protein_id="HAQ1659046.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            4514..4801
                     /locus_tag="IXG44_002351"
     CDS             4514..4801
                     /locus_tag="IXG44_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1659047.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
BASE COUNT         1693 a          813 c         1030 g         1391 t
ORIGIN      
        1 ttaaaagtat aatttaatta attaagtttg ttttaagaag agaacttttt attaagtttt
       61 tttattttga ggaggaattt gaatgcaaga gctgaagacc cgcagtgccc ggcaccatag
      121 acctaaaaga aaaatggtga tcaataaaaa agcgattgct cctttaattg gaacgagtct
      181 tttagttggg ccgatgatgg ttacagcggt accgcaacat gttcatgcag atgaacaagg
      241 atctcaaaca gtcgctgatc ctcaagcgtt tatcgatcaa atcggctggt ctgcgtcaga
      301 agttgctgca agtaatgact tgtatgcttc agtcatgatc gctcaagcgt tgttggaaag
      361 tgcatatggc acttccggat tagctagtgc gccaaattat aatctattcg gtgtgaaagg
      421 aagctataac ggacaggtcg tttatatggc aaccaaagaa tatgtaaacg gacaatgggt
      481 aacgatgaat gagccattcc gaaaatattc ttcttattgg gaatccttcc aagaccatgc
      541 gaatgttctt cgttcaacca gtttttccac gggcgattat cattatgctg gcgtgtggaa
      601 aagcaataca acaagttttt atgatgcaac agcgtacttg acaggtcgtt atgctaccga
      661 tccaaattat ggggccaaat tgaactcatt gattaatact tataatttaa cccgttttga
      721 tacgccatca acaggttcgg ctacaacaac cacaagtaca acaacaacga ctactacgac
      781 gacttcaacg tcatcatctt caacaacaga tgtaaataca aataatggtc agacatatac
      841 agtcgtgtca ggtgatacgc tatgggatat tgcacaaaaa tttggcatgt ctgttgatga
      901 tctaatggcg aaaaatggca tgtctatgtc cagttattct ttattagttg gtcaagaact
      961 aaacgtttga tttagataaa aaggcaaaga aattaccata tatggtatat ctttgtcttt
     1021 tttttgctag tcaaagacga atcactcgcc ataaatgaac agattgtatt atgataagta
     1081 agaagagcaa agacaaggag gaattgaaga tgacagctat ctataactca gtcaccgaat
     1141 tgattggcaa aacgccaatc gtcaaattaa ataaaatcgt accagaagat tcagcagatg
     1201 tttttgtaaa gcttgagttt tttaatccag gaggaagtgt aaaagatcgt attgctttaa
     1261 gtatgataga aaaagcagaa cacgatggtc tactgaaacc aggtgatact atcattgaac
     1321 ctacttctgg aaatacagga atcggattgt cgatggtagg agttgcaaaa ggatataaag
     1381 taatcatcgt gatgcctgag acgatgagta tcgaaagacg tttattgatg aaagggtacg
     1441 gcgcagagtt gattttgact cctggtgcgg atggtatttc tggatcaatc agagaagcag
     1501 aacgtttggc aaaagaaaac ggctactttt taccattaca atttgaaaat gaagcaaatc
     1561 ctcttgtgca tgagaagacg actggtcctg agatacatca agcatttgga gtaaatggct
     1621 tggacgcgtt tgttgcaggt atcggtacag gaggaacgat cactggcgct ggccgagaat
     1681 taaaacgtgt gtatccgaaa atcgaattaa tcggggtaga accagcagaa tctgctattt
     1741 tagaagggaa agaagcggga cctcacaaaa tccaaggaat cggtacaggt tttgttccta
     1801 aaacactgga tacttctgtg tatgataaag tcctttctat tagtggagac gaagcgatgg
     1861 aaacagctag agaagttggg cgaaaagaag ggattttagt aggcatctca tctggcgctg
     1921 caattgctgc cgcgctaaaa gtcgcaaaag aattaggaaa aggcaagaag gtattagctg
     1981 tggttcccga caatggtgaa cgttatttat ctacagccct ttaccaagag atataaggca
     2041 cttggttttt ctccagatag gtttatgtaa aaaactttta aagatgaaaa gaacacacaa
     2101 aaatttatga tgagaaaaat tatttgaata gagacgtaga atagtgaacg cgactgtttt
     2161 acgtcttttt tcattaaaat acaataaaat tgaattttct tttcatgtta agtggatttt
     2221 aaaatgtaaa aatagtatga taagacgaga ataacaacgt ggggtgttta catggaaaat
     2281 aagttagtcg aagaggttat cgaaaagtta aaaaaagaga acattcgaat cacacctcaa
     2341 cggtacgcgg ttttagaata tttgattgaa catcattccc atccaacggc tgatgagatt
     2401 tataaagcac ttgaacatcg gtttccaaat atgagtgttg cgactgttta caataacctt
     2461 cgcttattta cttcgattgg atttgttcaa gaaatgaagt acggcgatgc ttctagccga
     2521 tttgatttca gttcaaagcg tcattatcat gtaatttgtg aaaactgcgg taagattgtt
     2581 gattttcatt acccagggct tgaagatata gaaatggctg caagcagact gactgatttt
     2641 gagattaatg aacatcggtt ggaattgtat ggactctgtc cggattgtaa agagtcagca
     2701 aaaaaggcgc aaatgtaaaa acagtttcta caagtgttat acctaaaaaa agctagctgt
     2761 gtaaaaaaaa cgctagcttt tttatttttt tgtcttttat tcacaatctt agatataaaa
     2821 gtgaatatta ttaaaataag aatcttgtag atattgggtt cttatgcttg aaaaggaatg
     2881 aaactacaaa caaacctttg tgaattcaca agcattaaac tgttgtatcc gttttatttc
     2941 tgttatagta gttttgtcga aggacaaaat gttaatcgga gggaccataa atgaaagtag
     3001 tagtaattgg ctgtactcat gcaggtactg cagctgtaaa aagtatttta aaaaatcatc
     3061 cagaagcaga agtgacagta tatgaaagaa atgacaatat ttctttctta tcttgcggta
     3121 tcgcacttta tgtcggcgga gtagtaaagg acccagcagg cttgttctac tcaaatcctg
     3181 aagaattatc ttcattagga gcaacagtta aaatggaaca cgatgtaaca gacgtcgata
     3241 cagaaaataa aacagtaacg gcaaaagatc tgaaaacagg agaaacagaa acggtttctt
     3301 atgataaatt agtgatgaca actggttcat ggcctattat cccaccgatt agtggaatcg
     3361 atgcgaaaaa tgtactccta tgcaaaaact acaatcaagc aaatgaaatt attgcacaag
     3421 cgaaagaagc aaaacgtgta gtaattgttg gtggtggata tatcggtatt gaattagtag
     3481 aagcttttgt cgaatctgga aaacaagtga cattgattga tggattagat cgcatcttga
     3541 ataaatatct agataaacca ttcacagata tcttagaaaa agaattgaca gaccgcggtg
     3601 ttactcttgc gctaggcgaa aatgttactg aatttatcac agatgaagaa ggaaaagtga
     3661 aacaagtagc tacaccaaca gatacatttg atacagatat ggtcatttta tgtgtgggct
     3721 tccgtccaaa tacaaaactg atcgaaggaa aagtagatac actgccaaac ggggcaatca
     3781 aagtgaatga atacatgcag acaagtaatc cagacatttt tgcagctgga gactctgcag
     3841 tagtcaacta caatccaagt ggcacgcaaa actatattcc tttggcaaca aatgccgttc
     3901 gtcaaggttt gcttgtggga aataacttga cagatcataa aatggcgtat cgtggtactc
     3961 aaggaacttc aggactttac ctatttggct ggacgatcgg ttcgacaggt gtgaccaaag
     4021 aaagcgcaac tttgaacgat ctagaagtcc aagcgactgt atttgaagat aattatcgtc
     4081 cagagttcat gccaactact gaaaaagtaa tgatggagct agtctatgaa aaaggaacga
     4141 atcgtatcgt aggtgctcag ttcatgtcca aatacgacat tacacaatca gcaaatacca
     4201 tgtctttagc tgttcaaaat aaaatgactg tagaagatct cgcactttca gatttcttct
     4261 tccaaccaca ttttgaccgc ccatggaatt acttgaacct acttgcacaa gcagcgctag
     4321 gcgaaatcga aactgcacaa taaagaaaaa gggttgtgac acaattttgt cacgaccctc
     4381 tgtctttttt tgtgaaaagt acaattagtt gtctttcttc gaattttttg tcacaataag
     4441 cagcaaagat aaagctgtta cataaaagaa caagcagctt tatttgattt tgaaaagaaa
     4501 ggtgagaaag aagatgaata aagaatattt tgtccaacaa gatgcatttt tagaagatta
     4561 taaaagtgct tcgaagaaat taacgatcaa tattgatgag ttggcagatc aagtactctc
     4621 tcagttgaat gaaacgcaag aggaatatta cacgatgcca gcaaatgacg ccaaagataa
     4681 tgtttctcat cgttttcctt tcagtagacg catctactca gatgaaaatg acggcacatt
     4741 gaatacagag tacacatatg aaggtcagcc atatgtgcta gaaactgatg atgatcttta
     4801 aagacagttt ttgaaaaggc cgctcatcct taagtgatga caaaaaatca tcacaattac
     4861 cgatgactgt ctctttacta tcttttaatc agaaaaaggg gttatgacaa gctttgtcaa
     4921 ccccttt
//
LOCUS       DACEUZ010000145         4904 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.145, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000145 DACEUZ010000000
VERSION     DACEUZ010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4904
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.145"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            <1..659
                     /locus_tag="IXG44_002352"
     CDS             <1..659
                     /locus_tag="IXG44_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HAQ1659048.1"
                     /translation="IMQVLSSSLLTPIIFILAFALGGEIKLTDLDNKLIGVVFLVGIA
                     LAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEKFRFGWILQSFLTGGIALL
                     SGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLLNWTNINQLFNRGSGSLGL
                     VATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLILSALWIVYYQRTFWKKFE
                     "
     gene            complement(910..1389)
                     /locus_tag="IXG44_002353"
     CDS             complement(910..1389)
                     /locus_tag="IXG44_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HAQ1659049.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(1405..2157)
                     /locus_tag="IXG44_002354"
     CDS             complement(1405..2157)
                     /locus_tag="IXG44_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HAQ1659050.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            2274..2344
                     /locus_tag="IXG44_002355"
     tRNA            2274..2344
                     /locus_tag="IXG44_002355"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2306..2308,aa:Gly,seq:ccc)
     gene            2705..3430
                     /locus_tag="IXG44_002356"
     CDS             2705..3430
                     /locus_tag="IXG44_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HAQ1659051.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            3685..3951
                     /locus_tag="IXG44_002357"
     CDS             3685..3951
                     /locus_tag="IXG44_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HAQ1659052.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            3948..4379
                     /gene="ruvX"
                     /locus_tag="IXG44_002358"
     CDS             3948..4379
                     /gene="ruvX"
                     /locus_tag="IXG44_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HAQ1659053.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            4404..4709
                     /locus_tag="IXG44_002359"
     CDS             4404..4709
                     /locus_tag="IXG44_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HAQ1659054.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
BASE COUNT         1614 a          883 c          966 g         1441 t
ORIGIN      
        1 tgattatgca agttctttct tcttccttgt taactccaat catttttatt cttgcttttg
       61 ccctaggagg cgaaataaaa ttaactgatt tagataataa gttaatcggt gttgttttcc
      121 tggtagggat cgcattggct ttcttaacag ttaatcagac ttcttttatc agcaatctta
      181 tttctttgga tcaagaaaat tttctgttta tccgttcttt gccaatctct atgaaccaat
      241 atttaaaaga aaagttcaga ttcggatgga tattgcagag ttttttaact ggtgggatcg
      301 cattattaag cggactgtct tttcagctgc ctttgttttt tatccctagc ttgctatttg
      361 gttctttatt cggctgctac ttattgtcac tacgttattt cgctagagat tatcggttgc
      421 tgctgctgaa ctggaccaat attaaccaat tatttaacag aggttcagga agtttgggtt
      481 tagtcgcaac tatgatgggt tcattgatca tcagtattat tcttatcgtc ttatacggaa
      541 tgttggcttt gttttatcca ttttggttag tgaatattcc tgtgatcggt ttagttctaa
      601 tactttccgc tctatggatc gtttattatc aacgaacgtt ttggaaaaaa tttgaataaa
      661 gaagaggggc cgtgacaaaa atcatgtcgc agcccctttt ttacagatag agggtgttca
      721 aaagtcagtt tctcagtatt aagtcaattt tatgtaaaaa tgtgagaagc tatttttaca
      781 aaactttttt taatactcat aattacttct tattgctcct gcggttactt gtcgcaatgt
      841 ttgtgagata ttttgtcatc gctcaaggct gaacgtctct ttcacaacct tttctttgtt
      901 acacttcgtt tatttaaaaa gccgtttttt tcgtttcatc atgattgcgt cacgctttac
      961 tttgaaatta cggatgttat ggttaaagac ttctgataat acaagaccta aagcgattgc
     1021 tccagcaatc ataattgctt ggaccgcata atgagccgct tctcgatagg attcagtaac
     1081 gagatttcga accgcttgat aagctaaacc accagggact agaggaacca tcccgggaat
     1141 attgaaaatc gttacgggca ttttcaaacg tttagagaaa agatcgctga tgaatgctac
     1201 tcctaaagaa ccaagcaatg aaccaaaagc agcagatccg cctatttgaa cacagatcca
     1261 gtagatcatc cagccaaaag caccagaaag accacaacaa atcaatgaac gcctaggtac
     1321 atttgtaata acggcaaatg ccgcagtcgc taaaaaacta aaagaaaact gaataaatat
     1381 cgtccccata cttgtgttcc tttcctaata aaaaagctgg aaaataaaag caatcgcaaa
     1441 accgatcatc gtagcagtca tcatcgcttc tgttccgcga gaaactcctg agacataatg
     1501 tccagctagc aaatcacgga tagcgttcgt aatttgaact ccaggaacta aaggcatcac
     1561 acatccgata atgattagat cctgattgct tcctaagcca aaacgtacgc tgtagatagc
     1621 ggctatgcca atcaataaag aagataaaaa ttccgataaa aatttgattc taaaaaattt
     1681 caaactatat agataagctg cgtatcccaa tcctccaatg actaaagtca caggtaaatc
     1741 ggtcatcttt ccgccaaata acaccataat tgttccactg atcactgccg cgctaacgat
     1801 ttgcaaccag acaggaaaga aagaacggtc tcggtcgatt gcttgcagtt cgcagtaaag
     1861 ttcttctaat gttagctctg aagcaacata tttacgtgaa agatcattta cttttactac
     1921 tttttctaaa ttaatcgaac ggttggtaat ttgttccatt cggatggtcg aggtacgatc
     1981 aagaccaata aagagaccgg tttgagtgac atagctgacc aaacggaaat ttccagaagc
     2041 taaagcaatt cgactcatcg tatcttctac ccgatacatc tccgcattgc tttccatcat
     2101 gattttccca gcgagtaaac aagtgtccag caacagatct aattcttttc ccgtcatagc
     2161 atcttctcca ataattgatt gattagtttt attttactac aaaacatacc agaagcgtaa
     2221 agaataaaaa aacttgagga aggaataaaa gaggcgtata ctgtttttgt attgcgggtg
     2281 tagtttagtg gtaaaatccc agcttcccaa gctgatgtcg cgggttcgat tcccgtcacc
     2341 cgcttaaaat aggttgtgaa aaagctgctc tgcaacaagt aataagaaca gccaagcaaa
     2401 aacaacttct tatgttttta acttggatgg acttattacc gcagatgcaa gcttttgcgc
     2461 accgtttacc aagcggttgt gaaatcagtg tattgtcttg agtatgaaga aaatttttga
     2521 ataataataa ataccttaca gatagagtgg tgactgatta gagacgagga atccgtctct
     2581 tttttgtatt tttaagccat cttaaaacag gttttatttt ttgctgtttc tatttctttc
     2641 tagtatttat ttatagggga acatatgtta agatttaggg agctgttagt aaaggagtaa
     2701 agaaatgagc aaaaaaacaa ccaaggcgat cgtggggatc gtcgctgctg tgattgcagg
     2761 ggcattcggt gtttccacca cacaaaaaga ttcagatatc tttcagcaga ttacaactat
     2821 ttttgattcc acaccacaag cttctcaagt agcggatgtg ccagaatatg atggaaaaca
     2881 tcaagaaata gaaatcaatg ggaatcagcc ggaatttaca aaagaggatc ttagcctttc
     2941 caaaggaaca tgggaaagct attcaaatat tgataaattg aacagagtag gacaagccaa
     3001 tgctatgctt ggaaaagatt tgttccctac tgaaaagaga gagcctttgt atattgaccc
     3061 gacaggttgg aaacaaaaga aattaagtga cgggcaatgg ttatataatc gcagccattt
     3121 aattggtttc cagctgactg gtcaaaataa caacattaaa aacttgatga cagggacacg
     3181 ttcgttgaat gctccataca tgcttgctca cgaaaatgat attgcggcat atatcaaaga
     3241 aacaaatcat catgtacgct accgtgtcac tcctcatttt gaggggaatg aactggtagc
     3301 caggggagtg caattggaag cagaaagcat tgaagacaaa aaaattgaat tcaatgtctt
     3361 tatctacaat gttcaagatg gctacacgat caattatgag acaggacaag caacaaaaac
     3421 aaaaaattag tagcagctat tcgaattttt tatgaatatt atgaaaaaat tgtgtaaaaa
     3481 catacattca aagaaatata gcgtatactt tatgaaaaga gtacttttga taaagctaca
     3541 gaaaaaagga aaaaaacata caaatagtgt ttcttaattt attcggaagt ttggaatatt
     3601 tgtttgtagt tttccctttc ttcgagtaaa ataaggttgt agaaggtaag ctggaattac
     3661 cttgcgggaa agaggtgtag aatcatgggt tttacagatg aaaccgtacg ttttggtttt
     3721 gacgatagtc gcaaaaaaga agtgagcgaa acattagcga ttgtttatcg ggctttggaa
     3781 gaaaaaggct acaatccaat caatcagatt gtcggctact tgctttctgg agacccggcc
     3841 tacattcctc gttatcgtga tgctcgaaat ttgattcgcc gtcacgaacg cgatgagatc
     3901 atggaagtaa ttgtcaagga ctacctctca aatcatggag tgaatttatg agagtaatgg
     3961 gcttggatgt cggttctaag acagtaggtg ttgctatcag tgaccccttt ggctggacag
     4021 ctcaaggagt agagatcatt cggatcaatg aagcagaaga agagtttggc tttgatcggt
     4081 tgaaggaatt agtagcacaa tatgaagtag accggtttgt agttggatta cctaaaaata
     4141 tgaacaattc catcggacca agggcagaag cttccatgtc atacggagaa aagatcaaag
     4201 aactattctc cttgccagtt gattatcaag atgaacgatt aaccactgtt caggcagaac
     4261 gtatgctagt agagcaggca aatacttcaa gagccaaacg aaaaaaagtc atcgataaat
     4321 tagcagctgt gatgatctta caaaattatt tagacggctc aggtaagcaa ttattttaat
     4381 aataaaagaa aagaggtaat cttatgacag aacataacca ttcccacgac catgaaggac
     4441 acgaacacat cacattggtg gatgaccaag gaaatgaaac attatatgaa atcctactaa
     4501 cagttgacgg acaagaagaa tttggacgta actatgttct tttatatcct gctggtgttt
     4561 cagaagatga agatgtagaa ttacaagctt atgcatacat cgaaaacgaa gatggaacag
     4621 aaggcgagtt agaacaaatc gaaacagacg cagaatggga catgattgaa gaagtattca
     4681 atacattcat ggcggaagaa gaagaataga acaaaagaat gaattaataa gatataaaaa
     4741 aagggattgt gacaaacgtc ttgtcacaat ccctttttcc aaataaacga tgttcaatca
     4801 gctgcccctc ggcattcgct cgaaaaacgg cgaaatatga agaactattt tgccatttgt
     4861 ttctctaata atcaggtcaa aacgttgact tcacagtctc ttcc
//
LOCUS       DACEUZ010000146         4807 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.146, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000146 DACEUZ010000000
VERSION     DACEUZ010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4807
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.146"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(89..1174)
                     /gene="xerS"
                     /locus_tag="IXG44_002360"
     CDS             complement(89..1174)
                     /gene="xerS"
                     /locus_tag="IXG44_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HAQ1659055.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLENNPMDQVATITDKTTLAARAN
                     AIEKKLFLDEDTQNYLDYIANEYKKTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VVNINLEDLSLEKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRVQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHTSTNTTTLYTHIDNAATIDALNAL"
     gene            complement(3250..3768)
                     /locus_tag="IXG44_002361"
     CDS             complement(3250..3768)
                     /locus_tag="IXG44_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HAQ1659056.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
BASE COUNT         1579 a          786 c          730 g         1712 t
ORIGIN      
        1 taaaaatgat aatgaagctt gcctttaaag ttgggctttt atctaatttt agagacaggc
       61 ttcattatta tactattttt tactacattt acaaagcgtt caaagcatca attgtagctg
      121 cattgtctat atgagtatat aaagttgttg tatttgtact tgtatgtccc aattgttggg
      181 cagttaatac caatgaattt gtctgttgat agagtctagt agccaaagta tgacgtaatt
      241 tatgtggact gacttggact ctgaatcctt ttgaatattt tgctaccatc cgttcgattg
      301 caataccgga tatacgcttg gcttctcctt gataaattgc tagaaataaa gctttttcgt
      361 tttctgttac tttgtatcgt tctttccgaa tagctagata attagctaaa tattccattg
      421 caaatgctgc tatatttact gcgtctctct tatttccttt tctagttact accacattat
      481 tcttttctaa agataggtcc tctaaattaa tatttacaac ctcggagagt cttaatccag
      541 atcctaaaat caatgcattg atcgccaggt ctctttctac atctcgttga taatagatca
      601 aagctcgttt gctcaaagtt tttttatatt cattggcaat ataatctaga taattttgtg
      661 tatcttcgtc tagaaataat tttttctcga ttgcgtttgc acgggcagct aacgtagttt
      721 tatctgtgat agttgctact tgatccatag gattattttc taagtatggt tttcctaaag
      781 gattattgga acttttcgaa agatatttaa atagaacctt taatgctgtg atatttctat
      841 tgatagtaga agtagataga cctaagttat tattatcttt gagcttagtt tttgttgtct
      901 ctttttttac attttccttt gtgcgacgtt tcaaatgaga tttgtacaag tttagatcat
      961 aggcagttaa ctgtcttatc gaggtgattg aagtatcggg aacgatagac tcactcagaa
     1021 gccattcata aaagagctta taatttttta gatattctaa agtagttcga ggtgattgat
     1081 ctttatcctc acgatcttga acaaaatctt ttatataatc gggcatatga agtatgattg
     1141 tatctatttt ttgatatatt tctgtatttt tcatgttatt catcctttcg ataagtaggg
     1201 gagaggagta tctctataat tggactatac caattataga gtgttttgtc aattggtata
     1261 ctctgaaatt tattaattta tttcaaaaaa gcagataaat aggtatagat ttaataattt
     1321 aaaataaatg gtatacatga ataggaaagg aaacagaagg aggcaatctt aattttttaa
     1381 attgtctaaa ttatatagaa agaacaatct ttctatataa ttagacataa gagagactac
     1441 ggttgatttg atattaacaa gcgtatatga atgtatatac aaataaaaaa ggggagggag
     1501 aagttaaaga aaattcagag aagtggggca ctcagtagta ttaacaattt aaaaggaatt
     1561 tcttaaagaa aatgggttgc atcctggaga tacaattttt ttagataaag aaaaaattca
     1621 agcaacaatt caaaaaaatc ttaactgctg agattgatgt agcaatatct caatcatcaa
     1681 caaagtacga tcctgccttt caaaagctta ttgataaatg tttattcatt ataaaaataa
     1741 aagaaaaatt tatgtcagct tttttcatca aagtggtaat gataattcgt ctattaattg
     1801 tattttttag gaaatatggt ataattgttt taacgaaaag gtaattcatg atacaccaaa
     1861 aaccactagc tggcgggcta gtggttttct tttacgttct attcagatta acgggtaggc
     1921 ttactgtctt ttttggcaat aattttgcaa tcaagccatt tacagatgta atgcgcggat
     1981 acttctgcta agattgcaaa aaacatatcg aaaaggtaat tcataataca acctcctccc
     2041 cttgccataa taggccgggg tagcagacag cataaatatt ataccataac tgaaaatttc
     2101 attttagtac atttcaatat attgatgaac cagcacaata aaacatgcca tgactaacag
     2161 aaaaaaactt attaaaccag atataacttg taaacatcca actatataga aaacgtaaat
     2221 tttctatata gttggattaa gataacgact atttatttta tcatattttt actataccct
     2281 atacttttac ttggtatatt tatgaataat cgcaaattta cccctatact actagtttgt
     2341 aagttagatt aataaaaata ctaaagttat ttgttctttt attaaataca cctacaactt
     2401 ataaattgat ggtatttaga taacacgaaa gaataaaaat aacagctgat agtaaagttt
     2461 gttgacgatt gctactcaat cgaaagtgag ttagtgcgta tgaacttgct tctgaaaact
     2521 tatgaaatga aagacctttt acatcttgca gaaatcggta ctgggattaa actaagcaat
     2581 aaaaactaat gttcccctca ttctttgaac ttctactatt tgatttattt attctgataa
     2641 atgacgattt caccttcagg atcttcaaat cccgtataga atttatttaa gatctcttca
     2701 gtatcagtaa taggataggc gataggccac ataccctttg atggttagaa taaactgctc
     2761 aaaatctgta tcgtatgttg gctttagcat attaacaaag gctatctcca aattataaac
     2821 cataagcgtt gaatcgttct taacaatttc aattcttcct aacccaaagt cagaagatat
     2881 aattaccgga cttgccttac ccttcatgtg ggttgttgag attccatatt taaagggaat
     2941 tgtcggtact atcggcggtc tgaaaaacat cccaaaaata aaaaaatctt tcccctgcta
     3001 gtctcctaag tccctttgta gcggatatgg caccataatc tcattttatc gaagtgagtc
     3061 atggtttatc tgttggccaa ccaaaattga tacaatctaa cgatgcaccc tctacggcat
     3121 tctaggggag tgcacttgca ttcacccaat aaaaaccggc agttagcaaa gaacttatgt
     3181 agtcgtatgt tcttactgtt aacatacaag aggtctcctt tccacaattg cttagcagta
     3241 aacgggaatt tacaaagcta atctcttcca ttttcttatc tttgttctaa aagtcccaaa
     3301 tggagcaact gtatttacat gaataaattt atatacttcc catacagccg ttttggttgc
     3361 ctcgtctgcc cattttcttt tgtgtgcagt gaatagatct tcatcactca ttgaatcaat
     3421 catcatgtta atatcatcaa tattacgatt gagaatttcc tctaattctt tcaatggtag
     3481 atgtgcatag gtatttgtaa accacttata taactctcct aactgattcc atttaaattg
     3541 atcagaaggc gttttaacag ataatccatt tctttcatca ttttcccatt ttagaattaa
     3601 attcgtccaa ccaacttgat atgctaaatt ttcagcaggt gttctatcca cttcctcaac
     3661 tcttttatcc ttcaattctt caggaatagt ttcaaattct aaaatatatt tagtataagt
     3721 cctatttatt tcttttttta attcttcttt atttttatat gttctcatta tattacctcc
     3781 ataaattcct aattgtctcc tttaattcat ctataatttc atccacgata ttttaatccg
     3841 ttctacaatg catatctgat atgcattgac tgtctaactc atatgtgaat gatttataaa
     3901 atatcccaac gtctcatcta aagaaatgtt gatcgtcagc aaggagctaa aaaatacgaa
     3961 tatatttctt ttgtagtaaa atcggactgg tttgcatatc attttcttcg taaatatcaa
     4021 aatcatgtta actactatca attactaata aaaactacca ctttaagcgt taagaactgt
     4081 taagtttagt taagtgagga tagttcacaa aatccattgt taaacttcac gaaagtggta
     4141 aaactcaaaa tgctttatat aggattacag catcgctctt tcttcgattg cgcgttgggt
     4201 aaaaaatatt atgaagtaaa aattgacgat gacacgttcc ttacggctca tcagatgaaa
     4261 cagctacaaa aacgaaatga tcaactcgaa gaaaatttaa ttctaaaaaa gcgattgcca
     4321 tattcacgcc tcgctcgacg aactattaaa ggtcaagatt tacctgaaga tcatcctttt
     4381 taggaaattg aagcttttta tgttgcacaa tgcatttata atactacaat gatgttttca
     4441 ccagatattc ttctagggtg cggtgtaatg aaacagatac acttgcaaaa gaaaattcac
     4501 cgcgaattta ctaaaattat gaataattat gttaatcttc cgaaattttt ttccaaaatt
     4561 aagggatcaa gctggtgtta tagattgttt aaggttggca aaaaaataac ttctttttaa
     4621 acaattatag aaaaccgata ctctgttaag ttggttgaca cggtgtcgat ttttatgact
     4681 ttcttattct gaaacttcac taacggacag tacctaaaaa gtctatagta tggacgttta
     4741 cgagtactat tttatcaaaa atgctgctca tttcaactta tatgttttag tgaactaccc
     4801 ccactta
//
LOCUS       DACEUZ010000147         4646 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.147, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000147 DACEUZ010000000
VERSION     DACEUZ010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4646
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.147"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(98..1228)
                     /locus_tag="IXG44_002362"
     CDS             complement(98..1228)
                     /locus_tag="IXG44_002362"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HAQ1659057.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDRLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     gene            complement(1877..3079)
                     /locus_tag="IXG44_002363"
     CDS             complement(1877..3079)
                     /locus_tag="IXG44_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1659058.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3139..3342)
                     /locus_tag="IXG44_002364"
     CDS             complement(3139..3342)
                     /locus_tag="IXG44_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAQ1659059.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3484..4551)
                     /locus_tag="IXG44_002365"
     CDS             complement(3484..4551)
                     /locus_tag="IXG44_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAQ1659060.1"
                     /translation="MLDSLQEEVVPVNRVLIDYLTIHFFSNQHLKLIEEIIEMPIERF
                     EELDYAPKGYIGQYVWNQVITIRYSIDDAVKGTVMEFSGQGCKHLARWLKTRKTNWQQ
                     FFRKVLDYQGNFTRIDFTLDDFVGSLSIPELKHKVILGHVWTTFQVSESHGGTDIINN
                     ESNGETLYLGSKKSQCRFCFYQKDHEQRKRRGIPLEEAKVKNRFELRYRKEKAQSLAK
                     IISKTHDLTKLFFELLNGAICFYDRDPNDLGAKVDAKWAAFISNYGAITISLDTIPQS
                     FEKTMSWLIHGVSPSLVFIAQVDQTFHSSLLPLIIEQGEMNPRQIKLLQNMKADPEYY
                     QEEVEYYERKLQSISNEKTSY"
BASE COUNT         1345 a          959 c          718 g         1624 t
ORIGIN      
        1 aataaataat ataaaaggta aataaaaaag agtggatttc tccactccat gaaaagccga
       61 ctttttggga cgcttttttt tgccaagcct cacattttta tagccaaact tcttttgcga
      121 cttgctgcaa taattggatt ttcttccatt gttcttcttc tgttaaatgg tttccttctt
      181 ctgttgaagc aaacccacat tgaggactga tacttagatt ttccaatggt acaaattgtt
      241 gtgcttcttt tattcgttct tttattttct ccgtatcttc caaatctgga aacttagaag
      301 taactaatcc taaaacaact ttactttctt tgttactcca atgtcttaaa ggagtaaaat
      361 ctcctgctcg gtcagaatcg tattccaaaa agaaaccatc ataatttgtt gaaaacaact
      421 cttctgcaat gggttcatat ccaccggaaa acagccaaga agaagcgtga ttcccgcggc
      481 agatatgtgt tgtgatcgtc aaatcttctg gtttgtcttc cagtgcgtta ttgataatct
      541 ctgtacaaat atgggccatt tctttcggat caattccttt ttgaagaagt gcttctcttt
      601 tagtttgatc agctagaaac gcccaatttg tatcatcgaa ttgcaggtaa cggcaacctt
      661 tttcataaaa agcatgaatc gctgctttgt aagtagagga caagtcctca taaaaatcag
      721 atagatcagg ataaatctcc ttgatttttg aagaaccatc attagatagg atctcttgtc
      781 gtaaaatcat cgtgggacta gggatcgttg cttttgcaac aaaagtatca tctgtttcca
      841 ctacttcttt cagaaaagca aaatctttta aaaatgggtg atttggattg aaagataatc
      901 tatctactac ccgaatatta taagctggtg ctgcttttcc gtggaatgtt tgattatatc
      961 cagtttctgg aacatagcct tcgataccat ttagttcttc taaaaaatca agatgccaaa
     1021 atccacgccg aaattcacca tctgtaattc ctttgatgcc gactgctttt tggtcttcca
     1081 ctaatttttt tatttcctgg tcttcgattg cacgaagttc ttcttttgaa agtaagcctt
     1141 cttgaacttt ttttcttgct tctttgatcg attgcgggcg taagaaactt ccgacatgat
     1201 ctactgtaaa tggtctattt ttgctcattt cactcactct tttctttgtt ttttgtaaaa
     1261 aaaaatcctt ttactagcaa atactagtaa aaggacgatt atactcgtgg taccaccttt
     1321 tttcagaaaa aacattcttt ctctcagcct gacaataaaa tcagtggtct tggtaacgaa
     1381 agactgttcg tttttgccta cctttcgaca aaaaatcctc taaggccatt ttcgacagtc
     1441 aacttctagc tttttacacc aaccaaagcc tctcttgaac cattttctgt ctactttcct
     1501 tctcatcgga tactattaga ataaaattag aatagcatgt ttgaaagaaa agtcaatagg
     1561 gaataagaac aaacaaaaac taatttgtta ttcttagtaa tttgtttgtg gaatacaata
     1621 aagcttttct aatatgataa ttgttcagtc gtcctttcat cttagatcac ttccttaaaa
     1681 tgatcatacc cgtttcaacc acataaaaaa ggacttaatt ttgaaaaatt tcccatcaaa
     1741 actaagtcct ttttattata gttatgcaaa tttgcttatt ttctcgctat ttccgataca
     1801 tcttgaacag gagcaattcg actgaaaaca ctggtgcaaa agcatttatt tgtcttatgg
     1861 tagaaccgtg gaaaactcag actggtaata aattgtcatt ttttgaaaga atagcgccct
     1921 cttgaacatt atcaatcgca cttaccactt gttgatctga ctttgcatat aagtgaccgt
     1981 aagttcctaa agtgatttga atattcgcat ggcccaatcg ttcttgaata gatagcggtt
     2041 gaatattctg ctcaattaga aatgcaacat gagaatgtcg tagcgcgtga agcttaatcg
     2101 gtttgacacc cgctttttta gcagcttctt ttaaccagtt cgcaaaagaa taaggtggcg
     2161 taaatgttcc gtccaactga gcaacatatt caaaatttcc aatacgtttt tgcaagtcat
     2221 accactgtct gagttttaag gaaagcttct tccccaaccc aatcgttcga taagcacatt
     2281 tagttttggg agtcgaaaag taccaatcat cttttgtatt ataaatcaat gtcttctcaa
     2341 tcgttatcgt gtttttctca aaatctacat tcgaccaaga caaagcttca agttctccaa
     2401 tccgaatgcc agtataaaag agcatttcat acacaatctt ccgatggtgg gtttgaatct
     2461 tatcatcctt caattcattc atcacttttt gaaactcact gtgttcccaa aactcaactt
     2521 tcttattctc agaaggaagc gaagaaacat cttcaacggg atttttagca atcaccccct
     2581 ctttaacagc ccgatccaat acaattttta ggtgccctcg aatccgattg atataagatt
     2641 ttgataacgt ttcgccatta acttttttcc gtgaattacg tgactctgtc tggcttagtt
     2701 caaataacca atcctcaata tcgtgacttg taatatctcg aacacgaaaa tgatagaaaa
     2761 actgcatacg cttttcgaaa atgaattgcg cattctcata tgtttgactt ttgacctgtc
     2821 tctcgtacca ttttacaaac ctatccttgt aaaactcttc gaaagtcatt tgattggtag
     2881 aacacagttg attaggaatt gtcttgttaa aatcatgtgt ttccatgtac ttgtcataag
     2941 caattttggc atctttttgt gaagcataac ctcgtttagt cttctgaacc tttcttccat
     3001 tttcatctat acctagatac gctcggaaga accatttttt tgtcttttta tcttggtaaa
     3061 tatttttaat ttttggcatc gttccattcc tccctgtact tgagaaaagc tactttaata
     3121 gtaccgcgct tgtactgttt attccgcaac ttgaatgtga aaaagatgtt caattactcg
     3181 ggatggcacc acaccaactt ggcgattatt ataaaaatca ataccttcaa tccttgcaag
     3241 atatgccttg ctctctttaa tcattcgtgc agcttgacta ggtttaaagc ccatctctat
     3301 caaaattgca cgatttacca tgaaatcaca ttttgtttcc attctctcac ctcctgattc
     3361 tttactcctt ttaaaataat cctctttata atctattgtt tctcattcga tggttgtgtt
     3421 atactcgtct tgtcgaggcg gagtacgttg cacaacttgt tgtgtaagca gtactttgct
     3481 tttttagtag cttgttttct catttgatat actttgtaat tttcgttcat aatactcaac
     3541 ctcctcctga taatactccg ggtctgcctt catattttgg agaagtttga tctgtcttgg
     3601 attcatttct ccttgttcaa taatcaaagg caacaaggaa gaatgaaacg tttgatcgac
     3661 ctgtgcaata aaaactaatg atggtgaaac accatgaatt aaccaactca tagttttctc
     3721 aaaactttga ggaattgtat ctaaagaaat ggtgattgcg ccataattgc taataaaggc
     3781 tgcccatttg gcatcaactt ttgcacctag atcatttgga tcacggtcat aaaaacaaat
     3841 tgctccatta agtaactcaa agaacagttt ggttaaatca tgggtttttg aaataatctt
     3901 tgccaagctt tgcgctttct cttttcgata ccgtagttca aagcgattct ttacttttgc
     3961 ctcttctaaa ggaattcctc gtcgcttccg ttgttcatgg tccttctgat aaaagcaaaa
     4021 acgacactga ctcttctttg agcctaaata caaggtctca ccatttgatt cattgtttat
     4081 gatatccgta ccgccatgag attcactcac ttgaaaagtg gtccacacat ggcctagtat
     4141 taccttatgc ttaagttctg gaatgctgag actccccaca aaatcatcta aagtaaaatc
     4201 aatccgtgta aagttcccct gataatctaa taccttacga aaaaactgtt gccaatttgt
     4261 ttttcgtgtt tttagccatc ttgctaagtg tttacagcct tgacctgaaa actccatcac
     4321 agttccttta accgcatcgt caatggaata ccgaatcgtg attacttgat tccacacgta
     4381 ttggccaata tagccctttg gcgcataatc cagttcctca aaacgttcaa tgggcatttc
     4441 aataatctct tcaattagct ttaaatgttg attcgagaaa aagtgaatgg ttagataatc
     4501 aatcaaaaca cggttaactg gaacgacctc ttcttgcaaa ctatccaaca caagattcaa
     4561 tttttcttca cttaattgtc tcgttccttt ttctactttc gataataact atctcgaaat
     4621 ccccactttt tctgctaaat atcgtt
//
LOCUS       DACEUZ010000148         4520 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.148, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000148 DACEUZ010000000
VERSION     DACEUZ010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4520
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.148"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            176..442
                     /locus_tag="IXG44_002366"
     CDS             176..442
                     /locus_tag="IXG44_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ1659061.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            586..2577
                     /locus_tag="IXG44_002367"
     CDS             586..2577
                     /locus_tag="IXG44_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HAQ1659062.1"
                     /translation="MATTHNTKKISMAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            2931..4232
                     /locus_tag="IXG44_002368"
     CDS             2931..4232
                     /locus_tag="IXG44_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAQ1659063.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYHYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
BASE COUNT         1364 a          797 c          998 g         1361 t
ORIGIN      
        1 cgctgatttc acaacctctg attcacggtg ttccagaagc agcttctacg gaaataagct
       61 gaacactttt gttacaactt ctttttttct ggagtttctt ggagcatatt tttggagtcc
      121 ttctgaataa agcgtaaaat aaaagtgaga aatatggata aaggagggaa aggagatgct
      181 gaatttttta tggtcgttat ttatcggcgg tatattaggt ttgatagctg gcgcaatttt
      241 aggaaaggaa gtcccgggag gcgtatctgg aaatatcatt atcggttttt taggtagttg
      301 gctcggcgaa tttttactag gaccactggg accggaactc ggcggttttt atttgatacc
      361 agcattattg ggtgcaattt tttgccttgc tctttattca tttgtggcaa gacgtatcag
      421 agaacgtcca aagaaagaat aattttattc aaataagttg acacttcagt gtgttacttg
      481 gataattaga agcaatttag gaaagcgggt gtcatggata agaatatcat ctatcattca
      541 agcttaccta aaggatatcc atataacaag aaatgaggga aaaacgtggc aacaacgcac
      601 aataccaaaa agatatcaat ggctggcctg cttgtagcga tgggagtcgt ctacggagac
      661 atcgggacaa gtccgcttta cgttatgaaa tcgattgttg aagataatgg cggattgtca
      721 ggaatcaatc ctgattttgt tatcggatca gtctcattga ttttttggac attgactcta
      781 ctgacaacaa tcaaatatgt cgtgattgca ttgaatgcag ataaccatgg tgaaggtgga
      841 attttctcac tctacacact tgtccgaaaa ggtgggaaat atttgatcat tcctgcaatg
      901 atcggaggag ctgccttact agcagatggg gtattgaccc ctgctgtgac ggtaacgact
      961 gcgattgaag gcttgcgagg aattccgcag ttttttgacc ggttcggtaa taaccagaac
     1021 gtgatcgtca ttatcacttt agttattatt tttgttttat tcatggtcca acgctttggt
     1081 acagatattg tcggaaaagc attcggacca atcatgttcg cttggtttac ttttttaggc
     1141 gtaatgggat taatgaactt cagtcaagac tggacagtta tccgtgcact gaatccttat
     1201 tatgctatcc agctgctttt tagtgcagat aacaaattag gcttgttcat tcttggaaat
     1261 attttcctgg ctacgacagg tgcagaggcg ctttattccg atcttggaca tgtcggcaaa
     1321 cgaaatattc gtctgagctg gccttacgtc aaaatttgtt tggttatcaa ttattttggg
     1381 caggctgcgt ggttgttgaa tgtttatcaa aaccctgaag cacagcaaat caagaacctg
     1441 aatccattct tccaaatgat gcctcaaagt tggatcgtga tcggcgttgc ttttgcaaca
     1501 gtcgctgcag tcattgcttc gcaagcattg attacaggtt cgtttacgct agtttcagaa
     1561 gcaatcaagt tgaaactgct gcctcggtta aagatcatgt atcctgggaa cagtatcggg
     1621 caaatgtata tcccagcagt caatctgatt ttatggatcg cctgttctct agtggttgtc
     1681 acattccgaa cgtctcatca tatggaagct gcttacggtc tatcgatcac tgtgacgatg
     1741 ttgatgacaa cagcactgct ttacttttat cttatccaaa atgggcattc tcgttggctt
     1801 gcatatttag ttaccttttt cttcggtgcg atagaagtcg ttttctttat ctcaagtgtt
     1861 gtcaaattct tccgaggcgg ttttgtagcc gttctgattg gtttgatcat cttagtcgtg
     1921 atgtttattt gggaacaagg gaacattatt cgcgaatcgt caaccgagga tgtgtctttg
     1981 agagattata tcccgcagct tagagaatta cgtgatgata cttctttacc aatctatcag
     2041 acaaatgtgg tcttcttggt tcctgatatg gctgaagata aagttggccg acagttcatc
     2101 tattcgattt tagataaacg gcctaaaaga gcacgagtct attggtttgt tcatgtggaa
     2161 gtgacggatg agccatatac gaaagaatat caagtagata tgatggacac tgattttatt
     2221 gtccaagtga atctgttctt aggcttcagg gtccaacagg aaatcaacgt atatgttcgc
     2281 caagtcatcc atgatttgat gaaacaaggc cgtttgccaa aacagccaca aagatactct
     2341 ctgacaccag ggcgagaagt tggcgatttc caatttgtcc tgattgatga agtagtttcc
     2401 aatgtgacaa ctctaggaaa atggcaacgt caaatcatgc aggcaaaact ggcaatcaaa
     2461 aaaatcgcta cctcgccaga aacatggttt gggctagagt acagcgaagt aaaacatgaa
     2521 actgttccgc ttatcattgg aaatactcgg aaaacatggt tgaaagagcg gttgtgaaag
     2581 tagcgctttg acctgaacta atgtcgaatg tcagctactt gaacaccgtt tatcagaggt
     2641 catgaaaaag ctgtactgca acaaacagta agaacgagca gataaaaaca gcttctcatg
     2701 tttttcatct gtttgggctt aatgccgcag atgcaagctt ttgaataccg tttttatcag
     2761 aggtcatgaa aaagctgttt agcaacaagt aacgcaatga ttttgaagtg ataaggagat
     2821 gtggctgaaa aatgccgcat ctcctttttt attcctaaat atgaatacgc attattttca
     2881 atctttttgt agaaaaaggg tacaatagaa aaagagaaag gcgggagaat atggttatca
     2941 atttcattat tttatttttg atgattgcaa ttacttcatt ttttgtagca agtgaatttg
     3001 ctttagtgaa aatcagacga tcaagattgg aacaactaga aaaggaaaat gtaaagaacg
     3061 ccaagctagc tcttcatgtt acacaccatt tagataacta tttatcagct agtcaattag
     3121 gaattacgct aaccggtttg atcatcgggt gggtcggtga aggatctgtc gcggctttac
     3181 tagaaccagt gatcggtaaa ctacctttta gttcggctat tagcagtaca atctctgtgg
     3241 cgctgggatt tattttagtt acgtatatcg atgtggttat tggcgaactg cttcctaaga
     3301 gctatagtat cgtcaatacg gagaaggtcg tcttgttcgt agttaaacca cttcattatt
     3361 tttataaagt aatgtttcct tttatttggg tattgaatca ttcagctgca gggctaggga
     3421 agctattagg tgtgcgtctc gtatctgaag gagaagagac attgtctcaa gaagagctga
     3481 cattagtcgc attgaattct tatgaaaaag gagagttgac caaagaagaa tatcattatt
     3541 tggaaaacgt gtttgaattt gatgatacct tggcaaaaga tatacaagta gaccgtacgt
     3601 ccatgcaagt attcgaagct tcagaaaccg tcaaagaagc gataaaagct tcgattgaac
     3661 aaggacatac tcgctatccg gttatcttag aatcaaaaga caatgtttta ggttatgtca
     3721 ccttgccaga tttgatcaag caatcttata aagatgacca gctgacagta gaacagttga
     3781 ttgaagagcc gatcgtaaca actgaaacga tccctataaa aaaattattg acgatcatgc
     3841 gaaaaaaggg aaagcatatt gctatcttaa aagatgaata cggagggaca agcggcttag
     3901 tgacgattga agatatttta gaagaaatcg tcggggaaat ccgagatgag acagatttgg
     3961 acgaagcatt gatagcggaa cagtcagatg gctcttatat tatctccggc aaactaacat
     4021 tagatgattt ccagcgttat tttcatgtag agattccaga gtttgaagaa acgaatttta
     4081 ctacacttgc gggctttgct tctagccgat ataaagaaat aaaagcagga acaatcattg
     4141 agattgcatc attccgtttc acggtattag aataccagca tgcacatatc gattatttca
     4201 aagtagagtc cacggaaaga aaaacagaat aaaagaaaaa aggctgtgac attttttgtc
     4261 acagcctttt tcctacgatt agtcaagtaa aaggtgcaag aaaaatagct cgtaaacgaa
     4321 agcgacaaaa tcaaagcaca ccaaataaag ccccgttcgt aaaaaatttt cgatctgggc
     4381 aaagaagtgg tatactgtaa gtggtttagt taatggtcca ctgagaccgc gtacgcatag
     4441 tcgtactctg tatgtgccct aaccatagcg tgcatacatt tcagaagctt gttcatacaa
     4501 gcaactgtgg caaccttatc
//
LOCUS       DACEUZ010000149         4511 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.149, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000149 DACEUZ010000000
VERSION     DACEUZ010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4511
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.149"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            <1..798
                     /gene="prmA"
                     /locus_tag="IXG44_002369"
     CDS             <1..798
                     /gene="prmA"
                     /locus_tag="IXG44_002369"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HAQ1659064.1"
                     /translation="DKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLA
                     IGKNKVTVSEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGM
                     AFGTGTHPTTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVA
                     VRSAKENMDMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQT
                     GTLIVSGIIEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            799..1563
                     /locus_tag="IXG44_002370"
     CDS             799..1563
                     /locus_tag="IXG44_002370"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HAQ1659065.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            1796..4009
                     /locus_tag="IXG44_002371"
     CDS             1796..4009
                     /locus_tag="IXG44_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HAQ1659066.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            4020..4466
                     /locus_tag="IXG44_002372"
     CDS             4020..4466
                     /locus_tag="IXG44_002372"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HAQ1659067.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
BASE COUNT         1583 a          793 c         1003 g         1132 t
ORIGIN      
        1 gataaaaatg attttacatc gctgaaagaa ggcgcactag tcatggctta ttttccggaa
       61 acgactttct taccggaaat cttgcctttt atcaaagatc gtatcacaca attacctgag
      121 tttggtctag ctattggaaa aaataaagta acagttagtg aagtagagga aagcaactgg
      181 gcaacagcct ggaagaaata ctatcatccg gtgcgtatta cgcgaatgct gacggttgcg
      241 ccaagctggg aaaactacca gacagaagat cctctagaaa aaattatcac actagatcct
      301 ggtatggcat ttggcacagg cactcatccc acgacttcct tgacattgac agcactagaa
      361 acagtcttgc gtggtggtga aacattgcta gatgtgggaa ctgggtcggg cgtgctaagt
      421 attgcagcaa aatatcttgg tgcaaaagat gtctatgcct ttgatttaga cgaagtagcc
      481 gttcgttcgg cgaaagaaaa tatggatatg aatgaaatag ccaaagatgt ccatgtatca
      541 gctaacgatc ttttgacagg aatcgagatt gaaagtgatg taatcgttgc gaacatttta
      601 gcagatatca tcttattgat gatcccagat gcttggcgtt tactgaagca aacaggcacg
      661 ctgatcgttt caggaatcat tgaagagaag aagcaaatgg ttctggatgc catgttagaa
      721 caagggttta tcgtggatca aattttccag caaaaagact ggtatgcaat tattttgaaa
      781 aaaccagagg tggaatagat gcagcggtat tttttaaaag aatcatatga atcaaaagac
      841 tattttgttt tagaagggga aaactatcac cacattgtac gggttatgag gatggaaccc
      901 gaggatgaag tttttttggc attcaatgat caaatagcca ttgttgcaga aatcacggag
      961 attactgaag aggctgttta tctgaaagaa attaaaaaag aagaaaatca aaaggaactg
     1021 ccaagttcga ttaccatagc gagtggctat cctaaagggg aaaagctgga ttggatcgtt
     1081 cagaaatcca ctgaattagg agcgcaagct tttcttggtt tcccggcatc agcctctgtc
     1141 gttaaatggg atgcaaaaaa acgtatgaaa cgacaacaac ggcttgaaaa gattgctcaa
     1201 gaggcagcgg aacaatccca tcggcagatc gtgccatcag tagagttatt ggaaacgaaa
     1261 caagatttat ttgagcggct agatgattat gacgtggtct tggttgctta tgaggaatca
     1321 gcaaagcaag gcgaaaaatc aaatgcagcc aaaattctta gtgaaacaaa accagatagt
     1381 cgaatcttag ctgtctttgg accagaaggt ggattatccc gcgatgaagt cgattatttt
     1441 gtagaacaca aagggatact ttgtggatta ggaccaagaa tccttcgtac agaaacagca
     1501 cctctttatt tattaagtgc tgctagctat caatgggaat taatgaatcc ttctttcagc
     1561 taacgttctt cattaataaa agagtatcga cagaaagaat tgaaaaaata gtcaacaatt
     1621 tgtataatag aaacaattat ttatacaatg gatacgaaaa atcgtttgaa aaagctttta
     1681 aaaagcgaac atttccgtaa aaaaacgtat aactgcacgg attttgggac aattatcaaa
     1741 cagatatttg tcccgattcc cgttttaccg aaaaaagaaa gggggatcga ttcctgtgac
     1801 aaaagaagaa attttaacgg gacccggtgt cataaagctc gttagtcaat acatgggtcc
     1861 cgaacatgta gcatttgttg agaaagcatg cgaatacgcg actgctgcac atgacggaca
     1921 gtttagaaaa tcaggcgaac cctacattat tcatcctatc caagtcgcag gtatattagc
     1981 agatttaaaa atggatcccc atacagtggc tacaggcttc ttacatgatg ttgttgaaga
     2041 tacagaaatc actttagaag atctgagaga agaatttggc gatgacgttg ctatgttagt
     2101 ggacggcgta accaaattag ggaaaataaa atataaatcc cacgaagagc agctagcaga
     2161 aaaccaccga aagatgctgc tagcaatggc tcaagattta cgagttatca tggtcaaatt
     2221 agctgataga ctacataaca tgcgtacgtt gaagcacctg cgagaagata agcaaagaag
     2281 gatcgctcag gaaactttag aaatctatgc gcctcttgca catcgtctag ggatcagccg
     2341 gatcaaatgg gaattagaag atacagcact tcgttatcta aatccaaaac agtattaccg
     2401 tatcgtccac ttgatgcaga cgaagagaga agaacgtgaa aaatacgtaa gtggtactgt
     2461 tgaagatatt cgaatagcga cagaagagct ggggattttt gcagaaatct atggacggcc
     2521 aaaacacatt tattcaatct atcgtaaaat gaaagatcag aaaaagcagt tcaacgaaat
     2581 ttatgacttg ctagctattc gagtgatcgt agattcaatc aaggattgtt atgctgtatt
     2641 aggagcaatc cacacaaaat ggaaaccaat gcccggcaga tttaaggatt atattgccat
     2701 gccaaaagct aatatgtacc aatctttgca tactaccgtg atcggtccgg caggaaatcc
     2761 ggtagaaatt caaatccgaa cacaggaaat gcatgaaatc gctgaattcg gggttgctgc
     2821 acactgggcc tataaagaag gaaaaaatga aaaagtagaa ccagatggta tgacgaaaca
     2881 attaagctgg ttccatgaga tactcgaact tcaagacgaa agctatgatg cttctgaatt
     2941 tatggaaggc gtaaaaggag atatctttag tgataaagtc tacgtcttca caccaaaagg
     3001 agacgttact gagttaccaa aaggatccgg accattggac tttgcataca gtatccatac
     3061 agatatcggt aacaaaacca ctggtgcaaa agtaaatggc aaaatggtgc agcttgatta
     3121 caaattgaaa aacggagata tcattgagat catgacttct ccaaattcat ttggcccaag
     3181 tcgcgactgg ttgaaattag ttgctactag caaagcaaga aataagatca aacgtttctt
     3241 caaagcccaa gatcgagaag aaaatgtgat caaaggccac gaatccgtgg tcaaatgtat
     3301 tacagatcta ggatttacgc ctaaagatat tttgacgaag aacaaactgc aagaagcact
     3361 cgatcgtttt aattatcaaa cagaagatga tctctatgca gctgtagggt atggagaagt
     3421 tagccccttg acgatggcca atcgtctgac tgaaaaagaa cgtaaagaac aaaaaatcga
     3481 gcagcaaaag caagaagcag aagaaatcat gaatcagccg aaaaaagaac ctgacaaaat
     3541 gaaagtacgt catgaaggtg gcgttgtcat tcaaggggta gaaaacttac taattcgtat
     3601 cagtcgctgc tgtaatccga ttcctggtga tgatatcgtt ggttatatca ctaaaggcag
     3661 agggatatcc attcatcgtc gagattgtcc gaatgttcag cctgacaaac caaatgtagc
     3721 agaacgtttg attgaagtcg aatgggaaga tacatcgaat acacgaaaag agtatgatgc
     3781 agatttggaa atttacggct ataatcgttc aggcttattg aatgatgtac ttcaaacagt
     3841 caatgcgcta acgaaaaatc tcaacagcgt tgaagcacgg acgaataaag ataaaatggc
     3901 gacgatccat ttgacggttg gtatccagaa tttatcccac ctaaagagta tcgtggataa
     3961 aatcaaagca gtacctgatg tctacagtgt acgccggacg aatggatagg aggacagata
     4021 tgagagcagt tgttcaacgg gtaagtaaag ctgctgtgtc agtcgatcag caagaaattg
     4081 gaaaaattgg ctacggccta cttgttttat taggcattca tgaaaccgat acgcaaaaag
     4141 atgtggacta tctggtaaaa aaaattgcac aaatgcgtat ttttgaagat gaacaaggaa
     4201 aaatgaatat tagtgtagaa gatgtgggag gacaaatttt aagtatctct caattcacac
     4261 tgtttgcgga tacgaaaaaa ggaaatcgac caagctttgt ggaagccgca cgaccagaaa
     4321 cagctattcc tttatacgaa tcattcaatg aaggaatcag aagtcgcggc atcacagtcg
     4381 ctaccggaca attcggagca gatatgtcgg tttctctcgt caatgatgga cctgtaacga
     4441 tcattattga ttctaaaaac aaataaagta atctggtttt cactagtaaa aactatgaaa
     4501 cacgtcgctt a
//
LOCUS       DACEUZ010000150         4488 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.150, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000150 DACEUZ010000000
VERSION     DACEUZ010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4488
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.150"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            93..422
                     /locus_tag="IXG44_002373"
     CDS             93..422
                     /locus_tag="IXG44_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ1659068.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            412..699
                     /locus_tag="IXG44_002374"
     CDS             412..699
                     /locus_tag="IXG44_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ1659069.1"
                     /translation="MAYEIVPSKHVIKYLKKLKEKPLKEKFLNLIYDEIAIDPYKGEQ
                     KTGDLSGIWSSGFKYAGTTYRVAYEIIENKVIPVLLCGTHENFYDQLKKLI"
     gene            985..1842
                     /locus_tag="IXG44_002375"
     CDS             985..1842
                     /locus_tag="IXG44_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ1659070.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            1843..2391
                     /locus_tag="IXG44_002376"
     CDS             1843..2391
                     /locus_tag="IXG44_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1659071.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            2651..3379
                     /locus_tag="IXG44_002377"
     CDS             2651..3379
                     /locus_tag="IXG44_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ1659072.1"
                     /translation="MKKFKKKIYLGLVVGVSMFGFMAHTKQVTADTNQKERIMSQLDD
                     SQIEYVPNELIVSLKATQRISTEKNKPQNLLGVKVMVEKDLTLRTNESDYNKRDQVLL
                     VKLNEEISVEEAVRTFNASDMVNYAEPNYVVELEPLNDVEEPNTTDSLGNRSIILNDP
                     NWSKQANLREISMPSAWEETKGSKDVKVAVIDSGIDYQHEDLSGNVDESLRYDFFDDD
                     TDPMDTMGHGHILPGLLVLKQTTN"
     gene            3409..>4488
                     /locus_tag="IXG44_002378"
     CDS             3409..>4488
                     /locus_tag="IXG44_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HAQ1659073.1"
                     /translation="MVPIRVFGSSGGTSLAILTEAINYASQKGFPIINFSIGSVVYSS
                     TEEVIKNYPGLFVAAAGNNGIDMTDKPFYPASLDIPNIITVGNSSGYERSSDSNYSKT
                     GVDLFARGFRVFSTFLGSKYTYMSGTSMATPHVTGSAALALSLDPDLSTDELKKIILN
                     SVDSFYELSDLCLTGGRLNTNNVVRQVTGKLTASNITLRLNSEWSDEIAKEMCHVHWI
                     DQSGNDLTDQVVVLKNEVDTSNFGDYEVVFASPDLTDQISVTVTVPRLRKLTLGKETA
                     TIDIGSAWDSVIAIDTFQVKAIDDFGNDVTNQVQVTINADIDTINSIQAVIKFEVPEL
                     GLAQIGRLNITAKNSTINGLNEVSIE"
BASE COUNT         1629 a          598 c          921 g         1340 t
ORIGIN      
        1 ttacgagtca cagtaattac ttgtaaatac attatctcgt ggtataatgt atttactaag
       61 taattacata ctgttagaaa tgagggattt aaatgattac aaaaacaaga aaacaaggca
      121 attcaatcat gttgacagta cctaaagatt ttaatgttcc taatggagtc gaagttgaag
      181 caaagttggt agagaatggt attctttatg aatttgttga accacaaaaa gaattttatg
      241 attttagcga agatattttg tctgatataa ttgcagaagg ctacgataaa gatgagattc
      301 ttgtagaatt taagaataga aaaaacaaaa tgcattcttc ctttagagat attgctgaag
      361 atacacttac taattctaaa gtaatgacta aagaggagtt ggcaacagaa attggcttat
      421 gaaatcgttc cttcaaaaca tgtcattaag tatttaaaaa agctaaaaga gaagccattg
      481 aaagaaaaat ttttgaattt aatttatgat gaaattgcga ttgatccata taagggtgaa
      541 caaaaaactg gggatttatc aggtatttgg tctagtgggt tcaaatatgc gggcacaaca
      601 tatagagtag cttacgagat tatagaaaac aaggttattc cagtattgtt atgtggaacg
      661 catgaaaact tttatgatca gttgaaaaaa ttaatctagt gatttgttgt cgttcttaag
      721 aaggacggct tcttttttat cgaaaatctg attcgtctac atgactaaag tcgcgagtat
      781 tctctgcttc tcgataaatt aaataacgtc attactttga ggaaagctat aaatcaatat
      841 atttcagctt ttccttattt atgtactacc ttttccttat taattataag gtagtaccta
      901 tatatctttt ttttggtagt aactaaaaat aaatatgtta taatagtatc aaatgaatat
      961 tgctaaaaga aaagaggtag gaacgtggta gcgaagaaat atgtaatcgg taattttaaa
     1021 ggtggcgtag gtaaaagtac gtgcgcgcaa atgttagggt ttgaatcagc agttaatgaa
     1081 ggaaaaagaa ccttgataat tgacttggat atgcaaggaa atacttctga tgtgatgaac
     1141 ttaacgcata tgaacttcac taacgaagaa ggaggcggag aaggtgaacc gattgtttac
     1201 gaaaatactg tgactgacgt gttgataaac aatcttccac ctaaagaggc tgtctataaa
     1261 gtaatcaata acctctatat acttccagca gacatgtcct ttgaaatgta tgatgattgg
     1321 atcaaagacc aatataatga ttcattatcc caattcaaat atatgaaaga aaagctagaa
     1381 ccattatttg atgagtttga cgtcatctat ttagatgtcc caccttctat ttctgtatat
     1441 agtaaatccg cgatgtacat tgcggactgg gcaatcgtcg tcttacagac ccaagtaaaa
     1501 tcgatgagaa atgcgatgca atatcttgag tatatggact tttttgtgaa agaatttgat
     1561 acaaatttat atgttgctgg tgttattcct ttcatgttgg aaagcggtga cgcagtagat
     1621 caggaaatgt acaaacaagc ccaagagatt tatggggaac atttgattaa aaatgttgta
     1681 ctcaaaaatg cacgcttaaa acgatacgac ggatcaggta ttacgatgga gaagacaaag
     1741 aaaggtctct taaaacaatg ggatcgcaaa agccacgaat tatttatcag tattttgaaa
     1801 gaattggaag aacacgagaa ttggtatgcg gaggtggagt aattggctga catcaaagga
     1861 cttgatattt ttgggaacaa caaaggactc gccaaaataa aaaaagaagc agaagcaaag
     1921 aaaaagcagc aagcaattac accatctgaa aataagcaat tagaagtgca ccaagttcaa
     1981 gaaataaaaa cttcctccaa tgttgaaaga cagagtagag gaagaacaaa aaaagttggc
     2041 gcaccaatca gaaagtttga taaggtttat tccaccaagc atcctttaaa actgtcgact
     2101 ttgttaaatg cgacttccag agttttagta gaaaagtatg agactaatat gacaagagat
     2161 gaattgctta gaaaagcttt agatgagtat attaaacaga atcttacgca agaagataag
     2221 caagatttgt ttaatgacgt ggtgaaagaa ctagacttgt tcagagaaaa atatccaatt
     2281 gttccagaat tagatgatcg tggagaaata ataagaacta ccagtcagat aaaacaagat
     2341 accgaagact cactgcgcaa agggtgggga atgaataata aagttagata ataatcaaag
     2401 aagaactaag taactacctt taaacggtag ttacttagtt cttctttgat ttgcgtgaaa
     2461 tagaggggat ttatttttct ggttacgtga ttaaaaaatc caaaaaatga gcataggaac
     2521 ggttatgatt ttagggtgtg ttttaatgag atattgtgat ttcattaaca agtgtataat
     2581 aatctgttgt tgagttagta aataagtaat agagctaact ctaaaataaa actattagga
     2641 ggagatgtag gtgaaaaagt ttaaaaagaa aatatattta ggattggttg ttggggtgag
     2701 tatgtttggg ttcatggctc atactaaaca agtaactgca gatactaacc aaaaagaaag
     2761 gattatgagc caattagatg actctcaaat agagtatgta cccaatgagc taattgtcag
     2821 tttaaaggct acacaaagaa tttcaacaga gaaaaataaa ccgcaaaatt tattgggtgt
     2881 aaaagttatg gtggaaaaag atttgactct taggacaaat gaaagcgatt ataataagcg
     2941 agatcaagta ttattagtga aattgaacga agaaattagt gtggaagagg cagtaagaac
     3001 atttaatgct tctgatatgg tcaattatgc agagccaaac tatgtggttg aactagagcc
     3061 gttgaatgat gttgaggagc caaatacaac tgattcttta ggaaatcgat caattattct
     3121 taacgatcct aattggagta aacaagctaa tttaagagag attagcatgc catccgcttg
     3181 ggaagaaacc aagggttcta aggatgtgaa ggtagcggtc attgattcag gaattgatta
     3241 tcaacatgaa gatctttctg gaaatgtaga tgaaagtttg agatacgatt tttttgacga
     3301 tgatacagat cctatggata ctatgggaca tggacacata ttgccgggat tattggtgct
     3361 caaacaaaca acgaattagg tgttgctgga ataaattggg atgttacgat ggtacctata
     3421 cgtgtatttg gtagtagtgg aggtaccagt ttggcaatct taactgaagc tattaattat
     3481 gcatcccaaa aagggtttcc aattatcaat tttagtatag gtagtgttgt ttatagcagt
     3541 acagaagaag taattaaaaa ctatcctgga ttattcgtag cagcagctgg aaataatggg
     3601 atagatatga ctgataaacc tttttaccca gcttctttgg atatacctaa tattattact
     3661 gtaggaaatt ctagtggtta tgaacgaagt tctgattcaa attatagtaa aacaggtgtt
     3721 gatttattcg ctagagggtt tcgtgttttt agtacatttc taggaagtaa atacacgtat
     3781 atgtcaggta cctcgatggc aacacctcat gtcactggta gtgctgcatt agcgttgtca
     3841 ctagatcctg atctatctac tgatgaatta aaaaaaatca ttttaaatag tgttgattca
     3901 ttttatgaac tatctgattt atgtcttaca gggggaagac taaatacgaa taatgtcgta
     3961 cgacaagtaa cgggtaaact tactgcatct aatatcacat taagattaaa tagtgaatgg
     4021 tcagatgaaa ttgctaaaga gatgtgtcac gttcactgga ttgaccagtc aggaaatgat
     4081 ttgacggatc aagtggttgt tctaaaaaac gaagtggata catctaactt tggcgattac
     4141 gaagtagtat ttgcatcacc tgatttaaca gatcaaatct cagttacagt gaccgtacct
     4201 cgattacgta aacttacgct aggaaaagaa acagcaacca ttgatattgg ttccgcatgg
     4261 gatagtgtca tagccattga tacttttcaa gtgaaagcta tagatgattt tgggaatgat
     4321 gttacaaatc aagtacaggt tactattaat gctgatatag atacaattaa ttcaattcaa
     4381 gcagtgatta agtttgaagt tccagaatta ggcttagccc aaattggtag actgaatatt
     4441 actgcaaaaa atagtacaat caatggattg aatgaagtta gcatcgag
//
LOCUS       DACEUZ010000151         4461 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.151, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000151 DACEUZ010000000
VERSION     DACEUZ010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4461
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.151"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            157..387
                     /locus_tag="IXG44_002379"
     CDS             157..387
                     /locus_tag="IXG44_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HAQ1659074.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            484..1785
                     /gene="murA"
                     /locus_tag="IXG44_002380"
     CDS             484..1785
                     /gene="murA"
                     /locus_tag="IXG44_002380"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAQ1659075.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1798..1974
                     /locus_tag="IXG44_002381"
     CDS             1798..1974
                     /locus_tag="IXG44_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAQ1659076.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2109..2405
                     /gene="yidD"
                     /locus_tag="IXG44_002382"
     CDS             2109..2405
                     /gene="yidD"
                     /locus_tag="IXG44_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HAQ1659077.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFQLTRNKKDKKEPDYKKVVKNTSSTER"
     gene            2589..3056
                     /locus_tag="IXG44_002383"
     CDS             2589..3056
                     /locus_tag="IXG44_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1659078.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVAPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3141..4025
                     /locus_tag="IXG44_002384"
     CDS             3141..4025
                     /locus_tag="IXG44_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ1659079.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNSYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4142..4375
                     /locus_tag="IXG44_002385"
     CDS             4142..4375
                     /locus_tag="IXG44_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HAQ1659080.1"
                     /translation="MSKKNKDVEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYGP
                     KEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
BASE COUNT         1524 a          678 c          969 g         1290 t
ORIGIN      
        1 tctcagtccc tttttttgca tatttttagc atttatttcc taaaaaatgt aaggggattc
       61 ctatattttt aagtatactt gtttttcctt ttatacattt tatggtatta tcattttatt
      121 agttattata aaacaatcgt tagaaaggac gtcattatgc aagtatacgg aattgatgcg
      181 gtgcttcgaa tcgtgagtca tttggcgttt atctacttag ccttctggtc tttacgttcg
      241 ttacgcattg aaactttttt cagaccaatg cataatacgc aaattagaat ggcaatcgta
      301 cttttttcaa tttttttagg gtacactgca agtaattttt tcttagaagt tatcgcttta
      361 tgccgaaatc ttttcgttag cctgtaaatt ttaaaaaaag gtactatttt aaaagcaagg
      421 atgttataat agaaaagatt ttgagtaagt gtatgctcat tggaattttt tggagggaac
      481 aacatggaag agatcatcgt aagaggtggc aatcaattaa atggaacagt aagaattgaa
      541 ggagctaaaa atgctgtttt gcctatttta gctgctagtt tgttagcaga agaaggtatt
      601 acgacattag acaacgttcc gattctttct gatgtcttca ctatgaatca agtgattcgt
      661 cacttaaacg tagatgtaga ttttgatgaa caaaagaatc aagtaacaat cgatgcttca
      721 agacaattag aaatcgaagc gccttatgag tatgttagtc aaatgcgtgc ttcaatcgtt
      781 gtgatgggtc cgctacttgc cagaaatgga catgcaaaag tagctatgcc tggtggatgt
      841 gcgattggta aacgaccaat cgatttgcat ttaaaaggat ttcaagcttt aggagcaaaa
      901 attattcaaa aaaacggcta tatcgaagca attgcggatg aattgatcgg taacacgatt
      961 tatttagatt tccctagtgt aggtgctact caaaatatca tgatggctgc cgtaaaagca
     1021 aaaggaacaa ccatcattga aaacgtagct cgcgaaccag aaatcgttga tttagcaaac
     1081 attttgaata aaatgggcgc tcaagtctat ggagctggta ctgaaacaat gcgtattgaa
     1141 ggcgtggatc atttacatgc agtcaaccac tcaatcgtac aagatcgtat cgaagcaggt
     1201 acctttatgg ttgctgctgc aatgacacaa ggcaatgtgc tgattgcaga tgctatctct
     1261 gaacataacc gcccattgat ttctaaattg atcgaaatgg gtgcagaaat cattgaagaa
     1321 gaaggcggag tccgtgtcat cggaccaaaa catatcttac ctacagatgt aaaaacgatg
     1381 cctcatcctg gtttccctac agatatgcaa gctcaaatga cagctatcca attagttgca
     1441 gaaggtacaa gtgtagtgac tgaaacagtg tttgaaaatc gtttccaaca tttagaagag
     1501 atgcgccgta tgaacgctca tgtcaaaatc gacggaaatg tagcaatcat ggatggaaac
     1561 catgaattgc aaggtgcaga agtatatgca acagacttgc gggcagcagc agcattagtt
     1621 ttagctggct tgaaagcaaa tggaatcaca cgtgtgcgta acttgaatta tctagaccgt
     1681 ggatattata atttccatat caaactacag caattaggtg cagacgttga acgtgtagac
     1741 atggatcaaa cttctgctga aaaaacagca caaacgattg cttaaggaag tggctgaatg
     1801 agtaaccaac gatatatctt attaactttg ataaagattt tagtggtgat cttattgctt
     1861 atccttcttt ttgtggccgg aacgatgatc ggatatggtg tcatcggtgg cggcaatcca
     1921 tttaaagtat tccagccaag tttgtggatt catattcgag acttttttca ttgataaata
     1981 agaatcacag tggttctagt cgatatgaag tgagccatga agcagaacat gacaaaagaa
     2041 aacagaaggg aacgttgacg ttagtcgcga tccctttttt tagtaaaaga aaagaaggaa
     2101 tctaattgat gaagaagata ttgatttatt tagttagagg atatcagcgc tttatttcgc
     2161 ctgcatttcc acctagctgc cgttattacc caacctgttc caattatatg ataaaagcaa
     2221 ttcaaaccca tggtgcacta aaagggacgg taatggggac tgcacggatc ttacgctgtc
     2281 atccttttgt caaaggcggt atcgattatg taccattgac attccaatta acgcgaaata
     2341 agaaagataa aaaagaacca gattataaaa aggttgtgaa aaatacgagt agcactgagc
     2401 gataaagaaa caatttggaa aatagcttct tatattttgc aaaattgtga gttattgccg
     2461 aagtgttgga ttttgaacac cgtcaaaaaa aggttgtgaa aaatacgggt agcactgagc
     2521 gataaagaaa aagaggaaaa ttattgtcaa agtaacaact ccgcaactac ttatatgggg
     2581 gacaaaaaat gatcgaagaa ctaaacaaga aagatttacc atggtccttg ttattagatg
     2641 cagatcctga taaagaaaaa gttcaagtat atgtttccag agggagtggc tggatatgga
     2701 aagaaaaaga aaaaacaata ggtgtactga tctatgtggc aagggaaaaa gaatacgaaa
     2761 tcgtcaatgt agcagtagcc ccaacgtatc aaggaaaggg gattggcgga aaattacttg
     2821 agacagcttt tcagaagctt tcacaactcg cttccagtca aactcagatc atcatacgaa
     2881 ccggcagtac tagcagtgcg gcgttacatc tctatcaaaa aatgggctat gtggagattg
     2941 gtcgagtcaa agactatttt atccatcatt actctgagcc catttttgaa aatggagatc
     3001 aattaaggga tcaagtaatt ttagcaattc acttacctaa aaatagcttt aactaataaa
     3061 ctttcaattc gagaaaatgc tttcaaatgc taaatagata cattacaata gctttagcaa
     3121 acaaaaagga gaatattttt atgggaatat tagtttttga ttttggtgga tcggctgtca
     3181 aatatggttg ttgggatggc aaagagatta aaggtaaggg acaatttgca acgcctgaat
     3241 cttgggaaga aatgaaagcg caattatttc aagtttataa aaaaatactc gtttcttttg
     3301 aagtcgaagg aattggaatc agctctcctg gagtagtgaa taatgagaaa caggtgattg
     3361 agggaatcag tgcgattcct tatattcatg gatttaatat tttccgagat ttggaaactc
     3421 tgtttcaact cccagtaacg attgaaaatg acgccaactg tgccggaatg gctgaatttt
     3481 atgaaggtgc tgcccgtgat tatcaagatg ttgcttttgt tgtggcagga actgggattg
     3541 gcggggcgtt gttcacgaaa ggacaaatca ataaaggggc acatctttat ggtggagaat
     3601 ttgggttgat gttcttaaat ggcgataaga cattcagtaa acttggtaca gcggtacaga
     3661 tggcttggcg ttattgtgaa cgcaaaggtc tcgataaaaa ttcttatacc ggaaaagatg
     3721 tctttgaatt agcagaaact ggagatgcaa tagcaaaaga agaagttgag agtttttata
     3781 cgtatcttac aaagggatta ttcagtattc aattttcgtt tgatccagaa gtgatcgtat
     3841 taggcggcgg tgtttctgca aaagaagggc tcattgatga gatcaaaagc cgaatgaaga
     3901 agctgacaga acaattcgat ctgcatgatt tcgaaccgca gattttatta tgtgaataca
     3961 gaaacgatgc taatcttgta ggtgcagcag caaactatct cgctgtacgt caaggggaga
     4021 attaagagct ttagagaagg tgcagcttgc ataaaagaag tgaatgaaat gaataggaga
     4081 tttttaccta aggaatgttt tatttgctgc agtatgataa aatatctgag aggtgaaaat
     4141 gatgtcaaaa aaaaataaag atgttgaagt gcgtatagaa gaagtgaaaa aagatatcaa
     4201 aggtaagaat tatgaagtca ctcagttatt tatagggaaa aaaatgattg gtgagatctt
     4261 agcctatggg ccaaaagaat atgaaatttt ctttggtgaa gaagattttg gaaaagaaaa
     4321 aagtcttgaa aatgcgatag aaacagtgat tcgacactgg aatctgcacg aataaaaaaa
     4381 tatcaaaaaa actttcaata aagggttgca aaaatctgga aaattaggta ttataactaa
     4441 gtcaggttga tacaaacctt t
//
LOCUS       DACEUZ010000152         4374 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.152, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000152 DACEUZ010000000
VERSION     DACEUZ010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4374
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.152"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(83..1051)
                     /locus_tag="IXG44_002386"
     CDS             complement(83..1051)
                     /locus_tag="IXG44_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAQ1659081.1"
                     /translation="MSKNGIIVNEWENLTALSNRRLNVQNMYTPLKMQWSIDRITIVG
                     KLKENIYYHTQNDVLILDFEQLMRVNEGNGYLKAVGNNGWQLLDQYEENIAYIEILKW
                     QDGKGRIDFNPNKIGHFLASSMKNFIHDLFLEPHFSRADIACDIIDVPDEFITQYRVV
                     DPVSFKPIYGRSGKLETAYWGSRASERQIRLYNKKLEQETKRKIVPPEIETWWRLELQ
                     LRRGKATDWHAMVHESLNSFASPHFLPLDVKTNDKVMIFGLIANPEMWGFLERRMKYK
                     LRDILKRESQNDELTNHLRETFSESADVLKKELDTWLQGLDVTEEE"
     gene            complement(1498..2904)
                     /locus_tag="IXG44_002387"
     CDS             complement(1498..2904)
                     /locus_tag="IXG44_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HAQ1659082.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDRQKTNDWHKHIDFTKSDEN
                     IYLEQTNLADKYEELFGEVVEKYNAKQKRADRKIEDYLAKVRKDKKLEPQREFIVQIG
                     ILDDFQTNRADGSSTGISKQQAEQNRVIANKILVQYYKEFQERNPNLAVYNAVIHNDE
                     ISPHLHLNIVPVAEGYKRGVQKQPSFNKALVQQGIHADKDNGRVLFNNFRNQEVDSIE
                     RLMNDYGWKRDIVGTNKIKDIHEYKEIMSEISELRQTAVKERESVSDELRYISRQREE
                     NERDKALMASERAKIEEEKKNMLNVKKEMAVIFQKDMVPLESFKRLPDGSYHLSGNDF
                     VDLYQRATRATNAKRNADNMSEQYLQMLEQNTELNEYVATLEEERHLSGRKQLADLQV
                     ENKKLKEENRSLRQRLERLKNAFQKSITRLSIRFGLAEKDMGLSEELEVLEQEKTLFY
                     RQNVSQRKLERSEDEIEW"
     gene            complement(3126..3488)
                     /locus_tag="IXG44_002388"
     CDS             complement(3126..3488)
                     /locus_tag="IXG44_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1659083.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKR
                     FGFETFADFERWLSSISDDQQHEKSVDNEWLADEKSAPSERDYPDESDGLFGVIPNKK
                     SQMSESKFTSIFNDVNLR"
     gene            complement(3492..3800)
                     /locus_tag="IXG44_002389"
     CDS             complement(3492..3800)
                     /locus_tag="IXG44_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1659084.1"
                     /translation="MDKIDLREETVDRIELMYERLADYLNLPDEDNYSEMLERMMDVL
                     NSVINADLAKSSVLTNEFVHKHLAQTTDESEKEFWLKLMSHVMQLNQISVINSKEKEM
                     "
     gene            complement(3809..4228)
                     /locus_tag="IXG44_002390"
     CDS             complement(3809..4228)
                     /locus_tag="IXG44_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1659085.1"
                     /translation="MTDLPEKMTIKNLADELGISKNTVRNQLVNAGYDLARYKDNGVI
                     MLDKELIGVVRSIYRSKGGQLTGDLPVNDAGNDFPLEKENEFLREQLAEKDRHISELT
                     EMIRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
     gene            complement(4221..4361)
                     /locus_tag="IXG44_002391"
     CDS             complement(4221..4361)
                     /locus_tag="IXG44_002391"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1659086.1"
                     /translation="MSGKLLDVTQYELILKNNKIDSPIIIPKHAVKYVYGVAESNGED
                     ND"
BASE COUNT         1234 a          994 c          619 g         1527 t
ORIGIN      
        1 aaaaaaacaa cgacaacgtt atcacctgtt aaattcacca accaattttt cttaccaaaa
       61 tgcttttgtt tttctgccat tcttattcct cctccgttac atctaaccct tgaagccaag
      121 tgtctaattc ttttttcaaa acatctgctg attccgaaaa tgtttctcgt aaatgattcg
      181 tcaactcatc attttgactt tctcttttca aaatatctct caatttgtat ttcattctac
      241 gctctaaaaa cccccacatt tcagggttag caatcagtcc aaaaatcatc accttatcat
      301 ttgtttttac atcaagtggt agaaaatgag ggctagcaaa actatttaaa ctttcatgaa
      361 ccattgcatg ccaatctgtt gctttcccac gccttaactg caattccagt cgccaccaag
      421 tttctatttc tggtggcaca attttacgct tggtttcctg ttccaatttt ttgttgtata
      481 gccgaatctg gcgttcgctg gcacgactgc cccaataagc tgtttccagc ttcccagacc
      541 gcccataaat tggcttgaaa gaaacaggat caaccactcg atattgtgta ataaactcat
      601 caggcacatc gataatatcg caagcaatat ccgcacgtga gaaatgtggt tctagaaata
      661 aatcatgaat gaaatttttc attgaactag ctaaaaaatg accaatctta ttcgggttaa
      721 aatcaatccg ccctttgcca tcttgccatt tcaaaatctc aatataagca atattttctt
      781 cgtattggtc taataattgc cagccgttat tcccaaccgc ttttaaatag ccattgcctt
      841 cattcacccg cattaattgc tcaaaatcta aaatcaacac atcattttga gtgtgataat
      901 aaatattttc cttcagtttt ccaacgatag taattcggtc aattgaccac tgcattttta
      961 agggggtgta catattttgt acatttaacc gcctgttaga tagggctgtt aaattttccc
     1021 actcatttac gattatgcca tttttactca aattttgttc ctcctcaaag aaaaagagac
     1081 ttctggaaag aattttcaaa agttcacgta actcttaaaa attcattacc aaaaaatctc
     1141 tagtcttgaa gaaatctaaa aatattcgta taatgattaa gaagttacgg agctagtgcg
     1201 attagtttcc gtaaaggtcg agaacgttgg tagcgttgct cgactatttt tttactcttt
     1261 tttcagttca tttgtttgct aactcaataa tagcacttct ctttagctat gcaaatatcc
     1321 aaaaaataaa accgcaccct ttgaaaaggg cgcccccaaa cttcgctaac gctaggttgg
     1381 ccaaaaaatg gactggccat tttttagcaa atgtcagctt aataggctag tggtttagcc
     1441 gtcaactcct tatcctcggc acaaaccgcc tccggtaccg ttgactgcac atcgtaacta
     1501 ccactcgatc tcatcttcag aacgctctaa tttacgctgt gagacgtttt gacgataaaa
     1561 taaggttttc tcctgctcta acacttctaa ttcctcagaa agccccatat ccttttctgc
     1621 aagaccaaaa cggatagaca aacgagtgat cgatttttga aatgcgtttt ttaatcgctc
     1681 taaacgctgg cgtaaagagc gattttcttc tttcagcttt ttattttcca cttgtaaatc
     1741 tgctaattgt tttctaccag acaaatgacg ctcttcttcc aatgtagcta catactcatt
     1801 taattctgta ttttgctcaa gcatctgtaa atattgctca ctcatgttat cagcatttct
     1861 cttggcattt gttgctctcg tagctcgttg atacaaatca acaaaatcat tcccagataa
     1921 atgatacgaa ccatcaggaa gcctcttgaa actttctaaa ggaaccatat ctttttggaa
     1981 aatgaccgcc atctcttttt ttacattcag catatttttc ttctcttctt caatcttcgc
     2041 tctctcagaa gccatcagag ccttatcacg ctcgttttct tctctttgcc tagaaatata
     2101 cctcaactca tcagaaacgc tctcacgctc ttttacagcc gtttggcgca attcagaaat
     2161 ttcactcatg atttctttgt attcgtgaat atctttaatt ttgttggtac cgacaatatc
     2221 ccttttccaa ccgtaatcat tcattaatcg ttctatagag tcaacttctt gatttctaaa
     2281 attattgaaa agcactcgac cattatcttt atcagcgtgt atcccttgtt gaaccagtgc
     2341 tttattaaag ctcggctgct tttgaacacc ccgtttataa ccttcagcaa ccggcacaat
     2401 attcaagtgc aggtgcggac ttatttcgtc attatgaata acagcattat aaacagccaa
     2461 attagggttt ctctcttgaa actccttgta atactgaacc aaaattttat tggcaatcac
     2521 tcgattttgt tcagcttgtt gtttcgatat gccagtagaa gaaccatctg ctcgatttgt
     2581 ctggaaatca tctaaaatgc ctatttgcac aataaattct cgttgaggtt ccaacttttt
     2641 atccttgcgc actttcgcta aatagtcctc gatttttcta tcggctcttt tttgtttcgc
     2701 attatatttt tctaccactt cgccaaacaa ttcctcatat ttatctgcta gattcgtctg
     2761 ctctaaataa atattttcat ccgatttcgt gaagtcaata tgcttatgcc aatcattggt
     2821 tttctggcga tcagttaact cacgattatt atgagaaata cttgtattct tcgttccttt
     2881 tttaaatgaa atactaaacc ccaagttaaa accctccttc gcgctgtcgc taataaaaag
     2941 attgtaaacc tttctgttac tctttgtcaa agagtaacat tttcctaatt gtgacatggc
     3001 tgtcacaatt ccaaatgctc tccgagggta ttcgctgtca caagtgacaa cgaaaaaagg
     3061 agccgaaagc gtgtcacgct ctcaactcct tttacgcttt attttgcttc acgcaaaata
     3121 atcgcttatc gtaaattcac gtcattaaat attgacgtaa atttactctc actcatctga
     3181 cttttcttat tcggtatcac tccgaataag ccgtcagatt catcgggata atctcgttcg
     3241 cttggcgctg atttttcatc agcaagccac tcattatcca ctgatttttc gtgttgctga
     3301 tcgtcagaaa tcgacgatag ccaccgctca aaatcagcaa aagtttcaaa cccaaaacgc
     3361 ttgcgaagag ccttgcccat tgcagcattt tctttctcaa tcaagcgttt tttctgttgg
     3421 cgaagtttcg cttgttcttt ttttaatttt tcttcttgcg ctttaatatc tgctaaagtc
     3481 attcccataa actacatctc cttttctttg gaattaataa cggaaatttg gtttaactgc
     3541 ataacatgac tcattaattt caaccaaaat tctttttctg attcatcagt tgtttgcgct
     3601 aaatgcttat gaacaaattc attagtcaaa actgaagatt ttgctagatc agcattgata
     3661 accgagttga gaacatccat cattcgttca agcatttcac tataattatc ttcatccggc
     3721 aaattcaaat aatcagctaa ccgttcatac atcaattcaa ttctatcgac tgtttcttct
     3781 ctcaaatcaa ttttatccat gtcacacctc acttccagaa ttgccaccat tttcgatttt
     3841 ttaattcttg attttctagt aacaactgat cgtttttttg ttgctgaagc aaagtcaatt
     3901 tttgttggtc acgaatcatt tccgttaatt cagaaatatg cctatctttt tccgctaatt
     3961 gttcacgcaa aaattcattt tctttttcta acgggaaatc attgcctgca tcattgaccg
     4021 gtaaatcacc agtcaattga ccaccttttg accgataaat tgaccgcaca acaccaatca
     4081 attccttgtc taacatgata accccattat ctttataccg agcaaggtca taacctgcat
     4141 tgaccagttg attgcgcact gtatttttag atatacccaa ctcatcagca aggtttttta
     4201 tggtcatttt ttctggcaaa tcagtcatta tcttctccgt tactttcagc taccccataa
     4261 acatatttaa cagcatgctt agggataatg attggggaat caattttatt atttttcaaa
     4321 atcaactcat actgcgttac atcaagtaac ttaccggaca tttttttgct atcc
//
LOCUS       DACEUZ010000153         4323 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.153, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000153 DACEUZ010000000
VERSION     DACEUZ010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4323
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.153"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(1..657)
                     /locus_tag="IXG44_002392"
                     /pseudo
     CDS             complement(1..657)
                     /locus_tag="IXG44_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333400.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
     gene            complement(662..1429)
                     /locus_tag="IXG44_002393"
     CDS             complement(662..1429)
                     /locus_tag="IXG44_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ1659087.1"
                     /translation="MNKKRRLEKILDMLKIDGTITIKEIIDELDISDMTARRDLDALE
                     ADGLLTRTHGGAQLLSSKKPLEKTHIEKKSLNTKEKIDIAKKACSLIKDGDTIFIGPG
                     TTLEQLALELKGRKGYKIRVITNSLPVFLILNDSETIDLLLLGGEYREITGAFVGSMA
                     STNLKAIRFAKAFVSANAVTHNSIATYSDKEGVIQQLALNNAVEKFLLVDSTKFDRYD
                     FFNFYNLDQLDTIITDNQISPQHLEEFSQYTTILKAD"
     gene            1918..2343
                     /gene="lacA"
                     /locus_tag="IXG44_002394"
     CDS             1918..2343
                     /gene="lacA"
                     /locus_tag="IXG44_002394"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HAQ1659088.1"
                     /translation="MAIVVGADLKGTRLKDVVKNFLVEEGFEVIDVTKDGQDFVDVTL
                     AVASEVNKDEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     VGAEIVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMC"
     gene            2360..2875
                     /gene="lacB"
                     /locus_tag="IXG44_002395"
     CDS             2360..2875
                     /gene="lacB"
                     /locus_tag="IXG44_002395"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HAQ1659089.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGHEVLDFGTYDHARTHYPI
                     YGKKVGEAVVSGQADLGVCICGTGVGINNAVNKVPGVRSALVRDMTSAIYAKEELNAN
                     VIGFGGKITGGLLMNDIIEAFIEAEYKPTEENKKLIAKIEHVETHNAHQADEDFFTEF
                     LERWDRGDYHD"
     gene            2886..3818
                     /gene="lacC"
                     /locus_tag="IXG44_002396"
     CDS             2886..3818
                     /gene="lacC"
                     /locus_tag="IXG44_002396"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HAQ1659090.1"
                     /translation="MILTVTLNPSVDISYPLETLKIDTVNRVKDVSKTAGGKGLNVTR
                     VLYESGDKVTATGFLGGKIGEFIESELEQSPVSPAFYKISGNTRNCIAILHEGNQTEI
                     LEQGPTVSHEEAEGFLDHYSNLIKQSEVVTISGSLPSGLPNDYYEKLIQLASDEGVAV
                     VLDCSGAPLETVLKSSAKPTAIKPNNEELSQLLGKEVTKDIEELKDVLKESLFSGIEW
                     IVVSLGRNGAFAKHGDVFYKVDIPDIPVVNPVGSGDSTVAGIASALNSKKSDADLLKH
                     AMTLGMLNAQETMTGHVNMTNYETLNSQIGVKEV"
     gene            3821..>4323
                     /locus_tag="IXG44_002397"
     CDS             3821..>4323
                     /locus_tag="IXG44_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose 1,6-diphosphate aldolase"
                     /protein_id="HAQ1659091.1"
                     /translation="MVLTEQKRKSLEKISDKNGVISALAFDQRGALKRLMAQYQDTEP
                     TVAQMEELKVLVADELTKYASSMLLDPEYGLPATKALDKEAGLLLAYEKTGYDTSSTK
                     RLPDCLDVWSAKRIKEQGADAVKFLLYYDVDSSDELNQQKQAYIERVGSECVAEDIPF
                     FLEILAY"
BASE COUNT         1431 a          681 c          845 g         1366 t
ORIGIN      
        1 gcttatgacg acttttggaa gtaaaaatcc atccattatc tttataatca ggattgaatc
       61 ttatatgttg gtgatttaac tcaatttcct ctttaagaat ttctatagcc cttttagata
      121 attggacatt ttggtaactt tgtaagtttt ttacattacc ttcatagcta ttttctgcag
      181 ctcctccgtg agtggactga ctaatgatga tattttgctt ttcaaaatat tgataggcac
      241 caatattttg gagttttcga ctctgtaagt caacagggcc attagttaga atacctattg
      301 ggatgcggtt tttatgtaga gccagtagta gttgatgtat ctcaggaatc atcgtaatct
      361 gatcgagttc atattcatag tcagcttgga agtctagagc ttctgtccta gaaatactat
      421 cgatgccaaa ttcttccata gtcttctgac agcgaaaaat tctaagctgc tcaatactga
      481 tgagtttttc gtgtatttag gaaaagcaac atcagaccaa catctgaaac gtttgtaaat
      541 gttatctatt tgttggatat caatcgttgg aaaacatttc tcaacgctac ttttaaaggg
      601 gagttgatgg tcataaagag tgtcatcaag gtcaaaaaca atatttttat aagtcataat
      661 tctagtccgc ttttaaaata gtagtgtact ggctaaattc ctctaagtgt tgagggctaa
      721 tctggttatc tgtaatgatg gtatcgagtt gatctagatt atagaagtta aagaaatcgt
      781 atcgatcgaa tttagtactg tctactaata agaatttttc tacagcattg tttagggcaa
      841 gttgttgaat cacaccttcc ttgtcactat atgtagcaat agaattatgg gtaacagcat
      901 ttgcactaac aaaagctttg gcaaatctaa ttgcttttaa atttgtcgaa gccattgaac
      961 ctacaaaagc tccagttatt tctctatatt caccgccaag aagcaataaa tcaatggttt
     1021 cgctatcatt tagaatcaag aacacaggga gactatttgt aatgacacga attttataac
     1081 ctttacgacc tttcaattct aatgccagtt gttcaagtgt agttccgggt ccaataaaaa
     1141 tagtatcgcc atctttgatt aaagagcagg cttttttagc aatgtcaatt ttttcttttg
     1201 tatttagact tttcttctcg atatgtgtct tttcaagtgg ctttttagag gaaagtaatt
     1261 gtgcaccacc atgagtacgt gttaaaagtc catcagcttc tagagcatca aggtctctac
     1321 gggctgtcat atcggaaata tctagttcat ctattatttc ttttatggtt atggtcccat
     1381 caatctttaa catatctaaa attttttcta atcgtcgttt tttgttcata tgaagaccct
     1441 tctttcataa agtaattttt ttccaaagat aattctcttt taattgtatc ataaaagaca
     1501 atattttcaa ggtaaaacaa acaatttcaa acaaaaacaa atgttagatg atgaaataag
     1561 aacagtggat tgacgtatat tagcttaggt cagattttgt ataagacgaa aataaagtag
     1621 gacctcttaa tcagtaagtt atagaaagta aaagactttt gtaatacttg aatagatatt
     1681 tcacgtccat tttgtgatgg attaaatgaa caaaaatgaa caataattta acggtgttat
     1741 ctatttttta aaaaaacaaa taaaaaaaca aaaaataaac aaaaaaagtg ttgctttttg
     1801 tttgaatgct tgatattata taaacaaaga aatgatgaaa acgttttttc gaacattttg
     1861 caaaatgttt tctacttcta cgtagcattt ctttctaaaa tttaggaggt ggtcaaaatg
     1921 gctattgttg ttggtgcaga tctcaaaggt acgagattaa aagatgttgt gaaaaatttt
     1981 cttgtagaag aaggttttga ggtaattgat gtgactaagg acggacaaga ttttgttgat
     2041 gttaccttgg cagttgcctc tgaagtaaat aaagatgagc aaaacttagg tattgtaatt
     2101 gatgcttatg gagctggtcc attcatggta gcaactaaaa ttaaaggtat ggttgcagca
     2161 gaagtttcag atgaacgctc agcttatatg acacgtggac ataacaattc acgaatgatt
     2221 acggttggcg ctgaaatcgt cggtgatgag cttgctaaaa acatcgctaa aggtttcgtc
     2281 aatggtaaat atgatggcgg acgtcatcaa atccgagtag atatgcttaa caaaatgtgt
     2341 taaggaaagg agttaaggaa tgagaatcgc aattggatgt gaccacattg tcacagatga
     2401 aaaaatggca gtatcagaat tcttgaaatc aaaaggacat gaggtccttg atttcgggac
     2461 atatgaccac gcacgtactc actaccctat ctatggtaaa aaagtcggtg aagcagtagt
     2521 aagtggtcaa gcagacttgg gagtatgtat ctgtggtaca ggtgtcggca ttaacaatgc
     2581 agtcaataaa gttccaggtg ttcgctcagc gttggttcgt gatatgacat cagctatata
     2641 tgcaaaagaa gagttaaatg cgaatgttat cggtttcggt ggaaagatta ctggtggtct
     2701 tcttatgaat gacatcattg aagctttcat tgaagctgag tacaaaccaa cagaagaaaa
     2761 taaaaaattg attgcaaaaa tcgaacacgt tgaaacacat aatgcacatc aagcagatga
     2821 agatttcttt acagaattcc ttgaaagatg ggaccgtggt gactaccacg attaaggtat
     2881 aacaaatgat tctgacagtc acactaaatc cttcagtaga catctcttat cctctagaaa
     2941 cattgaaaat tgatacagtg aatagggtga aagatgttag taagacagct ggtggaaaag
     3001 gcttaaatgt tactagagtc ttgtatgaat ctggtgataa agttactgct acaggtttct
     3061 taggtggtaa aattggtgaa tttattgaaa gcgaattaga acaatctcct gttagtccag
     3121 ctttttataa aatctcagga aacactagaa actgtattgc tatcttacac gagggtaacc
     3181 aaacagaaat tttggaacaa ggaccaacag tttctcatga agaagcagaa ggttttcttg
     3241 accactatag caatcttatt aaacaatcag aagttgtgac aatttcagga agtttacctt
     3301 caggacttcc caacgattat tatgaaaaac ttatccaact tgcttcggat gaaggagtag
     3361 cagtcgtttt ggattgttca ggtgcacctc ttgaaacagt tctaaaatcg tcggctaaac
     3421 ctactgccat caaacctaat aatgaggaac tttcacagtt gcttggaaaa gaagtaacaa
     3481 aagatattga agaactcaaa gacgttctta aagagtcact ttttagtggt atcgaatgga
     3541 tagtggtctc attaggtcga aacggtgcct ttgcaaaaca tggtgatgtt ttctataagg
     3601 tagatattcc tgatatccca gttgttaatc cagtcggatc aggggactca acggtagctg
     3661 gtattgcatc agctttaaat agtaaaaaaa gtgacgctga cttattaaaa catgcgatga
     3721 cattgggtat gttaaatgct caagaaacaa tgacagggca tgtgaatatg actaattacg
     3781 aaacactaaa ctcacaaatt ggagtaaaag aggtataaaa atggtactta cagaacagaa
     3841 acgcaaatct ttggaaaaaa tttcagataa aaacggtgtt atctcagctt tggcatttga
     3901 ccaacgtggt gctttgaaac gtttgatggc acagtatcaa gatacagaac caactgtagc
     3961 tcaaatggaa gaactaaagg ttttggttgc tgatgaactt acaaaatacg cttcttcaat
     4021 gttgcttgac cctgaatatg gacttccagc aacaaaagca ttggataaag aagcaggtct
     4081 tcttcttgcc tatgaaaaaa caggttatga tacatcaagt acaaaacgtt tgcctgactg
     4141 cttggatgtt tggtctgcaa aacgtatcaa agagcaaggc gctgatgccg ttaagttctt
     4201 gctttactat gatgtagaca gctcagatga acttaaccaa caaaaacaag cttatattga
     4261 acgtgttggt tctgaatgtg tcgctgaaga tattcctttc ttcttggaaa tccttgctta
     4321 tga
//
LOCUS       DACEUZ010000154         4320 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.154, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000154 DACEUZ010000000
VERSION     DACEUZ010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4320
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.154"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(<1..538)
                     /gene="sgrA"
                     /locus_tag="IXG44_002398"
     CDS             complement(<1..538)
                     /gene="sgrA"
                     /locus_tag="IXG44_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HAQ1659092.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTE"
     gene            complement(734..1573)
                     /locus_tag="IXG44_002399"
     CDS             complement(734..1573)
                     /locus_tag="IXG44_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAQ1659093.1"
                     /translation="MIRIIRLIGSVLIVFVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            1758..2645
                     /locus_tag="IXG44_002400"
     CDS             1758..2645
                     /locus_tag="IXG44_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rotamase"
                     /protein_id="HAQ1659094.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            complement(3239..3934)
                     /locus_tag="IXG44_002401"
     CDS             complement(3239..3934)
                     /locus_tag="IXG44_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HAQ1659095.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(3918..4316)
                     /locus_tag="IXG44_002402"
     CDS             complement(3918..4316)
                     /locus_tag="IXG44_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAQ1659096.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
BASE COUNT         1432 a          853 c          640 g         1395 t
ORIGIN      
        1 tttctgtgct actttcactg ctcgtactct ctgtactgct ttcaccgctc gtgttttctg
       61 tactgctttc actacttgtg ctatccgtac tgcttggcgg tccaacaact gtccaactgc
      121 ttggtttcaa attctccgtg atcgtcatct gtccatcata aaaaacaatc aaatcaactt
      181 tatctttact cttgatctca tcaatcgata gttctgcaga aatcattatt gtatactcaa
      241 taccagcggt ccattgtacg tcttttaaat aaacttcccc aataactttt tctgaacttc
      301 cattaccatc ccaaccatat gttagaacag catgtacatc actagcattc acgcttttgt
      361 ttggtttgac tggaatagta atgacttgtt gtgtcatgtc ataaacaatc cgatcagttt
      421 tcacagtaaa tgaaagattc tcatttgccc gttcatttgc agaagtactg attccaatca
      481 ttgctgcaat tatactcagc ccaagaataa caaataatac ggtcgctgtt ttcttcattt
      541 ctatcactct ccatataatt accttctatt tgataaagga attatataaa atgaaaatga
      601 tgagaacacg acagtcagtt gtgaaaatct aacaaaagat gtcgtgtatt cgcttgaatt
      661 tgtttacttt ttcgtttaat caatgacaga cttaaaaaag caagaccgat atcagccttg
      721 ctttcttcat cttttattga taaacagcag tgtatttctt catccgcttt gttttatatg
      781 catagagtaa caaaccaaca atcaagacta acaagaataa tgtagttgat atagatgctt
      841 cagccccaaa gtttccccca gtaattattg tattttgcga ttggatcaga gtggagaaaa
      901 aaccttgttc ttgcgttccg ctgactggta tacccaaaat agctccttgt gtccaattcc
      961 aagtaatatg agcaccaata gccgtaaata aacttcctgt actgtagata agtgcgctga
     1021 aaaagcaacc tgctaaaaag atattgaaaa ctgccagaaa tgtcatgtta ggattcaatc
     1081 cgtgcaaaag agcaaataat gcagcagatc caatcattgc tattaacgga ccaaatctaa
     1141 gcatctgatg ctgaaaaaag ccacggaata aaagctcttc tgatgccccc actgcaataa
     1201 ataaaataaa actcgttatc agcggcacaa tgtttgcgtt tcctgaatct aaccgatacg
     1261 cccccgtcaa atataatgga ctagtaataa gcagaaacac tccaattcca acaacgagac
     1321 ctcccgctaa atacccccag tcttttcctc gaacagaaac accataatct tctattcttt
     1381 ttccttcgac cttactagca agaaataaag ctagtaaaaa aacagctaaa tgtccgaccg
     1441 taccggcaat tgctccaatc cattcactat ttccaataaa catatccgca agtgcattat
     1501 tgacaagaat tcctaaaaca aaaccaacaa agactattaa aacagatcca atcaaccgaa
     1561 taatcctgat catctttcat ccctccttta tattaagttt aacaaccctt ttctttcttg
     1621 tctcgtgaaa actatgtaaa tgttctccat catagagaga atagattttt acgaactaag
     1681 aatatctaca gcaaccttac gaaaatgaaa gactgtctcc ctaattcttc tgttagactg
     1741 tttttaataa ggaaaggatg ggaaattttt tgaaaaaaca actactaatt atgtgccttt
     1801 cagcatgtgc aattttacta ttctcaggtt gtagcaagtc caatgaaacg attgcaacgt
     1861 ttaaaggtgg aacacttaca gttcaggatt tttatgataa cccaaaagtt aaatcaaata
     1921 atcaaacact attaaaaaaa atgattgtct tcaaagcatt tgatgatgta tacggaaaag
     1981 agatttcttc aaaggaagta acaaaagaat ataatgaaca aatcaaaaaa ctcggtccaa
     2041 actacaagga acaattaaaa gcagttggcc aaacagaaga gacatataag aaactcttta
     2101 aacagatgct ggctttccaa tacggtttga aagccaatgt aaaattaaca gataaagatc
     2161 tagacactgc ttggaaagaa ttctatccag aagttagtac acagatcatt ctattttcga
     2221 ctaaagaaga agcagataaa gcaaaaaaag aagcaaatga aggagaaaat ttctccaagc
     2281 ttgttcaagc ttatggaaaa aataaaacat taaaggaaac tgatggaaaa atgaattttg
     2341 attcaaccaa tccagagatt cctactgaag taaaaaaagc agcatttaaa ctaaaaaacg
     2401 gggaagtttc tgatatcatt cctgtaacag atccaacgac ttatcagcaa agctattatc
     2461 tagtaaaaat ggtaaaaaaa caggataaag gttctaataa agacaaatac aaatctgagc
     2521 tagaaaaaat tgctaccgaa gcaaaaactc ttgatacgga attcatggat aaaacaatcc
     2581 gtaaagtgat gaaaaaagac aatgtaacta ttaaagatcc ttatgtaaaa aatatattca
     2641 aataaacaaa tttgacccaa ttggggatgt ggcaaaattt gagaagtgat ttttggaaat
     2701 tccttcttat ttttgaggca aaccactttt gccacaactt catcggggcc tttttttatt
     2761 tgtaccaaaa caaatcactg gatttgttac aatcatgagg atataattta aaaaagtttt
     2821 ttatttctat cacatgaatc aatgcgccat taagtagatt ctttcatgct tccctatttt
     2881 ttcacttgcc gtatcgcttc aaaaacaact atcataaact caaactcgac tgaaaagtta
     2941 ttttaaaaaa gactaaaaat atcccttctg gacttgttct tcttattctg ttatactaga
     3001 gaaacaagaa agatattttt atgcgacact tttcatttgt ttttatgaaa tttattcaaa
     3061 actggcgtaa cttcagagtt acaaggatgt attagaggtt agggaataca tcgtttaaat
     3121 gagaaataag taatagttag taatagtcac ccaaacttta aattaatgaa tattgaatcg
     3181 tcagatagcc tcctcgagtt ttcgagaagg ctatcgttta ttttatggag aaattgattc
     3241 agaagtaatc agcgaccttt tttagttcac caatcgtttc tgcttggaaa gtttgcaccc
     3301 aatcttcttg gaagcccctc actgctttat caatcgaggg catctctaat acttgatcta
     3361 gtagctcttt acctaacgtt atcgcatgag ctccagatcg aatggctgta gtgacttgtt
     3421 cacaattttt gaaactagct gctaatattt ttgttggtct ttgcgtacgt tcgatctcag
     3481 tccgcaacga atcaatcact ttcatagcat caatgccttg attttgcatc ctgttataat
     3541 aaggtgcaat ataatctgct ccattattaa tagctaaata cccttgtaat tctgtgtaaa
     3601 tagctgtacc ggtaatcaaa taaccttctt ttttcaactt tttgatcgct tgaaggcctg
     3661 cttgattcac cggcaccttg ataaacgtat cttttcccag ttcttctatg ataactcgag
     3721 catcctctat catttcttct tccgttcttc ctattgtctg aatatggata ggaatctgtc
     3781 cgattatttt tttgatttct ttcatatgcg agaagaagtt tgacgctcct tcttttttca
     3841 gaattgtagg atttgttgtt acgcctgtta aattcaaaaa ctgttgacaa gaataaatac
     3901 tttttgtatt tgctgtatca agcatgattt ccatatagct ttcccccatt ctgatcctct
     3961 tgatcgattt taatgattgt cccctttctt aaatgcggca tttccttatc ttctacatac
     4021 attgttcctg gaagttccgg ctcatgagat ccatcaaatt ttatcgtgat atgtcctaat
     4081 tcattcaaat tagtttggac aacatctcct actgctgtga ttacatacat ttgtttatca
     4141 aaacagagct tcatatcaca agttatttct tttcttacct gattcaattc aatcttataa
     4201 caataatctg ttaatgtttc cggtgcttca tctccaaata aaatcattat tttttctttc
     4261 ctgaatactt ctgcttctat accaacatct gttacaaccg tttcaaagat cgtcatctct
//
LOCUS       DACEUZ010000155         4227 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.155, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000155 DACEUZ010000000
VERSION     DACEUZ010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4227
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.155"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(77..607)
                     /locus_tag="IXG44_002403"
     CDS             complement(77..607)
                     /locus_tag="IXG44_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HAQ1659097.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            712..1263
                     /locus_tag="IXG44_002404"
     CDS             712..1263
                     /locus_tag="IXG44_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ1659098.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            complement(1416..1889)
                     /locus_tag="IXG44_002405"
     CDS             complement(1416..1889)
                     /locus_tag="IXG44_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ1659099.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            2027..3499
                     /locus_tag="IXG44_002406"
     CDS             2027..3499
                     /locus_tag="IXG44_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ1659100.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            3496..4206
                     /locus_tag="IXG44_002407"
     CDS             3496..4206
                     /locus_tag="IXG44_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HAQ1659101.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
BASE COUNT         1323 a          758 c          821 g         1325 t
ORIGIN      
        1 atgaggaact atttttccgt ttctttctct aatactcagg tcagaacgct gatttcacaa
       61 cctctctttc acaaccttat tttactcgtt tataattttc atcgaagtat tttccgctac
      121 ggatatcatc tggcattcca tcgtatggtt cttcgctaat aacatcttca ttgtctatac
      181 ctgcatcacc catataagtg atcgtagcaa attcaggaac aagtaatttt ggataagttt
      241 catatttttc acgagctgct tccatttctg cgatatgttc gttttggaaa gtaacagtga
      301 tttctggatg ttcttcaacc catttaggga aaaagatcat tccgtgaagt ttcttttcat
      361 ttggcataaa gtccaatgcc acgtctgtcc ctgttggctc tgtccaagtg attttgaata
      421 cgccgtccgt caatttaacg atatcagctt cttggtcttt tacccaacgg cctttgacca
      481 ttccgccatg gatacggtaa tcaacagtat ggtcattttt ggcataccat tcatattccc
      541 aaccattgtc atacgtatag ataaagtgag ttccaataaa atcttccaat tctttaaatt
      601 ctttcatgtt taagcatctc cttataaatt aaaacatgta ataaactaca tgttttcgat
      661 tactcattta tgatagaatg gatttaaaga tttgtcaagg agggaaaact gatggcaaga
      721 aaaaatacac ttcgatatat tttgttaggg ttattaagta agaagaagat gacaggatat
      781 gaattgaatc aatcgttcaa aaatgaaata ggagaatttt ggcaagcaaa acatagtcag
      841 atttatcctg aacttgcaaa aatggaagag caaggaatca ttcagcatca agtagagatc
      901 acaggtgaaa aattggaaaa gaaagtttat gaattaacag aagaagggaa ggaacaattg
      961 gcagagtgga ttcatacacc caccaatgaa ctaccggtta atcgagacga atttgttctg
     1021 aaattgtatt tcgtcaaaga cgtgaatgat ccggcattag tagaaattat tcagcagcag
     1081 cgtgaccttc atgaagaaaa acgacttcat ctattagagc gaaaaaaaac gatctttcct
     1141 actgaaaaag aaaaacatga aaattacgga cactacctga ttttagcgca tgcgatcaat
     1201 cgggaaacag aatatgcaaa ttggctaaca gatgtattag aagaaagtaa ccgaaaaaaa
     1261 taaaaaaata tccgaacagt gatggagaat tcctttctat atgaaagaaa cgcagatcac
     1321 cgttcggata ttcttatgtt caaacaggcc ttttttcaag tatttatgta gcatctactt
     1381 attaaaaagc ttggtactta ggagattagt ggcgtttaga gttcttttaa caactgttcg
     1441 tagtcagctt tttcatcgac aggaaggagc ttgtaagtta attcgtctgt ctcgttgtca
     1501 tgaatgatgt acatcttagc attctcttct ttgaagttac cgatttgtaa aatcgtatag
     1561 cgttcgtcga tcaattcgat atctttgatt gcttttgaaa aaactttacg gacatggtgc
     1621 attttctttt ggcggatttc tgccttttct gcagcttttg acaaagtaaa tccgtcatct
     1681 aaatattttt tgatcagttc tacttttatg actgtaggta aacgatattt tctaggacaa
     1741 ttcgcatcag cttcgatcga acgaatgaat cccttttgtt cccaatacct taattgacgt
     1801 ggagacacac ctacgatatc acttaattca ctgatgccaa caaccaattg atcattttcc
     1861 aacaaacgtt taagggaagc cccaaccatt tttgtcactc cttttgtcct cttgcttccc
     1921 ttaattatat ccgtattaac ttatgtgtca agtaggttga caaatagcga atttaggact
     1981 aaagtattct agtagtttca gaaaaaattc agaaagaagg agaaatatga gtaacaacca
     2041 acccgtagat atttacggaa aaccatataa tcggacactt ttagttgttg tcttattaat
     2101 tggtacattt tgtaccgttt tgaatcagac cttattgacg acagcttttc ctacattgat
     2161 gaaagctttc gatatttcag cgtctgatgt gcaatggtta acgactggtt tcttgctagt
     2221 caacgggatc atgatcccaa taacagcatg gctgatcaat aaattcagtt caagaaattt
     2281 atatctttct gccatgacga tctttttgat cggaacgatt acttgcttta cagcacctaa
     2341 ttttagtaca ttactaatcg gtcgtttgat ccaagcttgc ggtgtgggag tatcgatgcc
     2401 attattgcaa aatatcatgc tatctatttt cccgccagaa aaaagaggat cagcaatggg
     2461 gatgtcaggg atcgttattg gacttgctcc tgctttaggc cctacattat ctggttatat
     2521 cattgataac taccattggc gtgacttgtt tggtatggta ctgccaatcg ttgttcttgt
     2581 cttggtttta gcattcttcc taatgaaaag tgttattcca ttgtcaaatc ctagcattga
     2641 tattttatcc gctattttat caacgattgg attcggtagt ttactttacg gattttctag
     2701 tgtaggagat gacggctggg gaagcttgaa agtaattggt ttcttagttg ttggtgtgat
     2761 tttcatcgcc ttgttctcat ggcgtcaatt acatttggaa catccattct tggaacttcg
     2821 tgtattcaaa tcatctacat tcacgattgc agctgtttta gctggtgtaa caaatatggc
     2881 gatggtaggg gcagaaatgg tcgtaccatt gtatattcaa aatattcgtg gagaatcagc
     2941 attccattct ggcttgatgt tgttgccggg tgcattgatc atgggggcta tgatgcctgt
     3001 gacgggtgcg attttcgata aatacggcgc gaaacgttta gccatcacag ggatgttcat
     3061 tttgacagcg gcaactgcac catttgcttt cttgacaaaa acgactccag ttttatatat
     3121 cgtgatcctt tatgcgatcc gtatgtttgg gatctccatg gtcatgatgc cggtaacgac
     3181 ttctggtatg aatgcattgc caaataactt gatcagtcat gggacagcgg taaataatac
     3241 attccgtcaa atcgcaagct cgattggtac agctattttg atcagtgtgt tgacgaatgt
     3301 gacaaaagca aatatcccaa gcaaatctgt attggataat acgccattag cttataaaga
     3361 tcaggcaatc aatgccacac tgtcaggtta ccatgccgcg tttatcgtag cgatcatctt
     3421 tgggattctt ggctttatta ttgccttttt attgaagaaa aaagaacctg ttctaatgaa
     3481 agcgggggat aatgcatgat tattggagtt ttgattgtca gcttgcttgc ttttgcttta
     3541 acgaacgtgt tcgctaaaaa aacatggcaa acctttttat ctttgatttt cggactaatc
     3601 ttcgtcgctt cactaagttt gatcgtcgct aatttgtcca atcactttgg aatggaaaaa
     3661 gtaacggaaa caaaaacaga aaaaattgtt tccagtgctg attcccaagg agcagatatg
     3721 cttttgtata aagctttagg gaatggaaaa gaaaaagtgt atctttaccg aacaaatgaa
     3781 aaacaagaaa aaccaaaagc aactggtacg gataacgaaa caaataaagt agaaaagaca
     3841 gacggcgatg ctgaaaaagt gacgaaaact acttattggg aatacaaaaa cgacatgtat
     3901 aaattctggt tcaatattgc tgataacaat cacgaatatg acagccgagt gaatacattc
     3961 aaaatcccag agacatgggt agaattaagt acggaccaag cagccaagtt agctgaatta
     4021 gtgaagaaac agcaaagtac gatggaaagt gaagcaaaag cttatgttca agacggtatg
     4081 gtaaaagcaa tgactgaaaa tccaaaaatg agcaaagcag agcaagaaca acgtacgaaa
     4141 gccttagctg ctgaattcca acaacaagca ttcgcaaaat tagtgaaaga agcaaaaggc
     4201 gaataatatt ttgaacaccg tttaagg
//
LOCUS       DACEUZ010000156         4226 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.156, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000156 DACEUZ010000000
VERSION     DACEUZ010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4226
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.156"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            <1..345
                     /locus_tag="IXG44_002408"
     CDS             <1..345
                     /locus_tag="IXG44_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1659102.1"
                     /translation="SNIKTSTKLICKLFKLDKISNIDDVKSKILTRKVSEPVKLTAQD
                     KKNGIVLYGDDAKYNYNWDTSSEAEVLSIPTTDTSGEKVTFKINFITAISKLEYGKQK
                     KRDNNDQYLPKK"
     gene            426..1046
                     /locus_tag="IXG44_002409"
     CDS             426..1046
                     /locus_tag="IXG44_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1659103.1"
                     /translation="MNINRVIKLIGAKNINKVRSEFIFPEDFFNWFFYQFSECKRLLG
                     ENYYLKGIGGFMGNVADEQNIIKGISEQTSELTVTKAFISNGESLKNVTARIRKENEV
                     EIVFGIDHECNSVIYVTQSANLILFENGCKDDFMVVYLYDILIPKLRSLYMSSESIFV
                     SEQKGSFSKKIGIEVIESIMCHNHITLEELEILIGQKNNNTKIQIS"
     gene            complement(1376..2602)
                     /locus_tag="IXG44_002410"
     CDS             complement(1376..2602)
                     /locus_tag="IXG44_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1659104.1"
                     /translation="MPTKLSNGKYKTNLRYPKKFREITGITSEKYQKVFPTRQLAIKA
                     ENAMKRKIETVLREENANSLELKGKIKFKEFYESKWLPRYELGQTTRSKRAPSYVTIS
                     NTKDIFRLHILPMFGEYAMNYLNSNTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQCITAPKKIALEERRIREGNQALSSEELIAWIEAVNTDLNNHLL
                     TLHDYTLFMLTLYLGDRKSETYALQWKYIDFEKQTVRLKHALDKYQKKKFTKGRKDTV
                     IQVPEIVMALLSKWKSVQAEQLLRLKINQTPDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPELAHLSPHKLRHTYATIARQGGANMNQISSALTHSDISTTRIYVNTPDVV
                     DKTVFEAFQRGLNKCD"
     gene            complement(2674..2832)
                     /locus_tag="IXG44_002411"
     CDS             complement(2674..2832)
                     /locus_tag="IXG44_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1659105.1"
                     /translation="MNKKQTCEFLGIDNNTLDKWIRIYNFPVIRIDGIVRFDIEDIKD
                     FMNTFKTI"
     gene            3520..>4226
                     /locus_tag="IXG44_002412"
     CDS             3520..>4226
                     /locus_tag="IXG44_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAQ1659106.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKELKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIG"
BASE COUNT         1449 a          591 c          691 g         1495 t
ORIGIN      
        1 tcaaatataa aaactagtac gaaattaatt tgtaaattat ttaagcttga taaaataagc
       61 aatatcgatg atgtaaagtc caagatatta acaagaaaag taagcgaacc agtaaagcta
      121 acggcacaag ataaaaaaaa tggaattgtt ctttatggtg atgatgcaaa gtataactat
      181 aattgggata cctcctcaga agctgaggta ttaagtattc ctactaccga tacttcaggg
      241 gaaaaagtca cctttaaaat aaattttata acggcaattt ctaagctgga gtatgggaag
      301 caaaaaaaga gagataacaa tgatcaatat ctacctaaaa aataacgaat taatgatgca
      361 tatgttaaag ttgttttttt gaattaaaca actcaattta ttcagttata tgttcatcgc
      421 agcaagtgaa cattaatcga gttataaaat taataggagc taaaaatata aataaagttc
      481 gatcagaatt tatttttcca gaagattttt ttaactggtt tttttatcaa ttcagtgaat
      541 gcaagcgact cttaggtgag aattattact taaaaggaat cggcggattt atggggaatg
      601 tggctgatga acaaaatatt ataaaaggaa tatctgaaca aacatcagag cttacagtaa
      661 ctaaagcatt tataagtaat ggtgaaagtt tgaaaaatgt aactgcgcga attagaaaag
      721 aaaatgaagt agaaattgtt tttggaattg accatgaatg taattcagtg atatatgtaa
      781 cacaatcagc aaatttaatt ctttttgaga atggttgcaa ggacgatttt atggtggtct
      841 atttgtacga tatactaatt ccaaaacttc gttccttata tatgtcttct gaaagtattt
      901 ttgtgagtga acaaaaggga tctttctcga aaaaaatagg tattgaagta attgaatcga
      961 ttatgtgtca taatcatatt acgctagaag aattagaaat tttgataggg caaaaaaata
     1021 ataacacgaa aatacaaatt tcttagaaat cattttttat tgagatatct agataaatga
     1081 tttattcttc aatcaaccaa ctcgccaagc tgaggtaatg ttttgaggta atgataacat
     1141 acaacttttt ataccctagt aaaactctat aaacaaagac ttattaattt cgcatgaaat
     1201 acattcctgt aaaatgcaat aaaatagagt tttgtttaac caggtctcaa aaaggctcgt
     1261 aaagcatctc aattctctaa acgttaatat tatttatata tcaatgtttc aaggcacttt
     1321 tcaattgttt gaaaagtgtc tttttcttgt ttaagaacac atttaagaac acatactaat
     1381 cacatttatt caatcctctc tgaaaggctt caaaaacagt tttatctact acatctggtg
     1441 tatttacgta tattctcgtt gtagaaatat ctgagtgtgt taaagcacta gatatttgat
     1501 tcatatttgc tccgccttgt ctagctatag ttgcataggt atgacgaagc ttatgtggag
     1561 ataaatgagc caattcagga tgtcgtcttt taattgaatt gattcgatag tttaggtaat
     1621 cagcgtgaac agggcaattt acttctccgg atggtttagt ataggtaaag agatactgat
     1681 ctggtgtttg attaattttt aatctcaaca attgctcagc ttgcactgat ttccatttgc
     1741 ttagtaatgc cattacaatt tcgggtactt gaattacggt atcctttcga ccttttgtaa
     1801 atttcttttt ttgatattta tccaaagcat gtttaaggcg aaccgtttgt ttctcaaagt
     1861 cgatatattt ccattgcaaa gcatacgtct cactttttct atcaccaaga tatagggtaa
     1921 gcatgaaaag agtatagtca tgaagagtaa gcaagtgatt attcaagtca gtgtttactg
     1981 cttctatcca agcaataagc tcttctgacg ataatgcttg attaccttcc cgtatccttc
     2041 tttcttctaa agcaattttt ttaggtgcag tgatacattg aataattttg gtagttcggt
     2101 taaattcaat ataatttagt atttctgcaa tatcaaacat agaacggaca tatcctttga
     2161 taatttttat atttgcgtat tctttcgatt tttttgttaa ttcatctgat attatctcag
     2221 tatttgaatt tagatagttc attgcatact caccaaacat tggaaggatg tgcaatcgga
     2281 atatatcttt agtattacta attgttacgt aacttggagc tctttttgat ctagtagtct
     2341 gacctagttc atatctagga agccattttg attcatagaa ttccttaaat tttatttttc
     2401 cttttaattc tagtgaattt gcattctcct ctcttaaaac tgtttcaatt ttccttttca
     2461 ttgcattttc ggctttaata gccaattgcc tagttggaaa tactttttga tacttttctg
     2521 atgtaattcc agtgatttct ctgaattttt ttggatacct cagattagtt ttatatttcc
     2581 catttgaaag ttttgtaggc aaaattcctc actcccttaa aaagtaaggc agtatatttc
     2641 gctattctta gtatatgcgg cgagaagaaa ttttcaaatt gttttaaatg tattcataaa
     2701 atctttgata tcctctatgt caaagcgaac tataccgtca attctgatta ctggaaaatt
     2761 atatatacga atccacttat ctaaagtatt attatcaatt cctagaaact cacaggtttg
     2821 ttttttattc aagtagactc tcaattttat aaaatcatct cctaaaaaag attagaattt
     2881 ttgaattgct ttgggtaagc acataggaat ttcacctctc cccagtttac ggcgagcctt
     2941 acggaagtat cattatttgc tgctattgtc atggcatttt gttttcccag aacaaaaaga
     3001 aatctctaag tatttgtcgc taatcaggtc atggcgtact tgagtttcta gctcgaatag
     3061 cagggatcgt tattcaaaaa ttgtatttaa ttgtcaatga tcaaaaatag gttttttcct
     3121 attcactatt aataacgata aacatgtata aaaagacaca ctacgaaaaa agttaaatgg
     3181 agtttactga ttttcatatt tgtatatgga acagacatag tcttgaataa tttttataaa
     3241 tgttttgatg cttatcaact tattcaaccc gctatccact ttaagtataa tttttccata
     3301 cgccaataaa gtagttttag gagcatgttc tcatttgaga acgctccttt tttatatatt
     3361 ttgaaagcta cttactatac gcgtagtttc aaatagtttt gtcattctaa tcttcagcaa
     3421 cagaaaaaaa tctaaattat aaaatttagg gttgactaaa atataatttt tgtataccat
     3481 atgatcgtga taatcattat catttagaag gagaaagtta tgttagaagt gaaaaagctt
     3541 aatgtttgtt ataacgattt tttagcattg aacgagattt ctttaaaaat acaagaaggt
     3601 gctttaacag gtattattgg tccaaatggt gctggaaaat ctacattgct taaagctatg
     3661 ttaaatatta tccctcatca gggagaggtc tccattaata atgaagatat caataaaaaa
     3721 ctggtgaaga ttgcatatgt agagcaaaaa gctgatattg attttacttt tccaataaaa
     3781 gtaaaagaat gtgtatctat gggaacatat gccggaatga aagtttttca aagaattaaa
     3841 aatgcagaat ggcaaagagt ttcaaaggct ttggaaaagg tcgatatggg aaaatattct
     3901 aatcatcaaa taggagaatt atctggagga caatttcaac gtgtactttt agcaagatgt
     3961 cttgctcaaa atgcggattt catattttta gatgaaccat ttgtagggat agacttagtt
     4021 agtgaacgaa taattatgga tacgttaaaa gaattgaaac atcagggaaa aacagtgcta
     4081 attgtgcatc atgatttaag caaggtaaag aagtattttg ataatattat tcttttgaat
     4141 cgaaatctaa ttgctcacgg aagtgtagaa tctgtattta atgaagagaa tttaaaaaaa
     4201 gcctatggag atactatatt tattgg
//
LOCUS       DACEUZ010000157         4150 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.157, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000157 DACEUZ010000000
VERSION     DACEUZ010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4150
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.157"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            558..3197
                     /locus_tag="IXG44_002413"
     CDS             558..3197
                     /locus_tag="IXG44_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ1659107.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(3365..4063)
                     /locus_tag="IXG44_002414"
     CDS             complement(3365..4063)
                     /locus_tag="IXG44_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAQ1659108.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIAAILLIMIPI
                     ILFATTIYDGSASVSDATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVIGLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
BASE COUNT         1259 a          790 c          905 g         1196 t
ORIGIN      
        1 aaaaagccga gctctcgatg agctcggctt tcttgtacct cggaaggatt caaataaaca
       61 gaaatgattg taactttcca tcatacatgc aaagcacggt gttgaatttc ttcatgttaa
      121 acaaaaattt tgttttcgtt acatcttcaa catttgaaac atctcttgtt cctattcaat
      181 tttcagacgt ttacgttatt caattgttac gtatcgcatt acttcttgtt tcggatactt
      241 cgtgatccaa acatccatac gcattcacta tttataggtt tgtactctac acgcatgtct
      301 gggtgaaacc tgtacttcta acaactttat ttgcttacac tgttctatcg agaaattctt
      361 ctgtctattt gatggtatcc tccttcctta catctatata atagcatcct ttggtagcgt
      421 ttgcaaatat aagcacttta aattttacgc tttcttaaca aataatcgat aagcccttta
      481 tttgaagtaa ataaaaaaag gatgttatag taaaataagt accttttttt gtacgaagat
      541 tttaaaggag gaataggatg gaagaataca agcagacaat agaagaagta aagaaggtaa
      601 cagaagcaga tccagatact gggttgtctt cacaagaagt ggatgaacgg cgaaaaaagc
      661 agggattgaa taaattcgat gaggcgccga aagaatcgat gatcaaaaaa ttctttcgaa
      721 gcttatccga ttttacaacg atcatccttc ttgtagcagc ggtgatttcc ttttataccg
      781 catttgctac agaacatggg gatttatttg aaggactgct gatcatcgct atcgttgtga
      841 tcaactcggt attggcgatt gtccaagaag gaaacgcaga aaaagcactg gaatccttgc
      901 aagatatgaa taaacaaacg gcaactgtca tacgagacgg caaagtgacc actgttgagt
      961 cagagcaact ggtagtgggt gatattcttg ttttagagtc cggtgatgct atttctgccg
     1021 atgcccgttt gatcgaagca tcacaattac gtgtagaaga atcggcgttg actggagaaa
     1081 gtgaagcagt tgaaaaagat gcagcattta ttgctgaaga agatgagtca ttaggtgacc
     1141 aacttaatat ggtatttaaa ggatgtacgg ttgctgcagg tcgtgggaaa gcgattgtca
     1201 ctgccattgg tatggctaca gaaatgggta agatcgcgga tcttttgaac gaaaatacta
     1261 tgcaaaaaac acctttacag gtccgattga atcagctagg caaacgaatc agtatgatcg
     1321 ctttagcggc tgcggcttta gtatttgtga ttggtgaact gcaaggagaa ccactccttg
     1381 agatgttcat gacttcgatt tccttggctg ttgcagctgt accggaaacc ttgactgtta
     1441 ttgttacgtt gacacttgca tacggtgtgc agaagatggc gaggaaacat gcgatcatcc
     1501 gacagttgcc agcagttgaa acgctaggaa ccgctaatgt tatctgctca gacaaaacag
     1561 gaacgctgac acaaaatgaa atgcgcgtcc gccgggtatg gagcaaagaa gatgaagtaa
     1621 cgaatatcga agattcaatg acgaacagcg cgatggaaat attgaaaatg gctgcactat
     1681 gtacagatgt gacggtcgag caagaagatg acgaccttgt tatcaaaggt aatccaacag
     1741 aagtagccat cgtaagggct gttgaagaga attatcatac aaaagcagaa ttagaagaaa
     1801 aatatccacg agtaaacgag cttccttttg attctgaacg aaaaatgatg acaacagttc
     1861 accaaatggg cgaaaaatac atctcaatca ccaaaggagc atttgatgtt cttgctccgc
     1921 gctttagttc aggcgatgta gaacaagcgg cgatcgttaa tgatcgcttt ggtaaacggg
     1981 cactgagagt gattgctgtt ggttatgcta cttatgacga agaaccgcaa gatatttctt
     2041 ctgaagcact ggaaaaagac ttacgtttga ttggtctgat cggaatgatc gatccaccac
     2101 gtccagaaag taaaggagca atcaagcgag cgaaaaaggc tggcatcaaa acagtcatga
     2161 tcactggaga tcatgtggta actgccagtg ccatagcgaa agaattaggt atcttgaatg
     2221 atccgtcaga agcactctca ggatcagaac tgcatcaatt atcagacgaa gaattagatg
     2281 cgcgtgtcaa agctctctct gtctatgctc gggtaacgcc agaagacaag atccggatcg
     2341 tcaaatcttg gcaacgtacg ggtgcagtcg tagcgatgac tggggatggt gtgaatgatg
     2401 caccagcgtt gaaagcaagt aacgttgggt gtgccatggg gatcacaggg acagatgtgg
     2461 cgcaaagtgc cgcagatatg attttgacgg atgacaactt tgcgacgatc gtagatgctg
     2521 ttagtcaagg gcgttcagtt tatcagaata ttcgtaaagc aatcaacttt ttactaagct
     2581 gtaatatttc ggagatcttt attgttttga ttgcgatgct gttaggatgg ggagcaccat
     2641 ttacagctgt gcaactactg tttgtcaatg tagttgctga tggacttcca ggatttgctt
     2701 taggaagaga gccagcggaa cacggcatca tggatcagcc acccattccg aaaaatgaag
     2761 ggatctttgc tcgaggttta ttgcaaaaaa ttgccataaa tgcaggggtc ttcaccatcg
     2821 taaccttgtt tggttattat ttaggtagtt atgtcgatac tatcagccca tgggtagacg
     2881 caagtcagca tgtcggtcaa actgttgcat tcttgatctt agcttattca tcgattctgc
     2941 atgtgttcaa tgtacgcagc agccaatcga tcttcaaagt caatttagca accaataaag
     3001 ctttggtaga aatggcgtta ctggcattag ctattacaac ggctgttgct ttgctgccat
     3061 ttacgcaaga actattcggc ctagtacaca tcagtttgaa tcactggttc ttggtaggga
     3121 ttttatctat cgtaccaatc gcggtgaatg aactgatcaa gttccatcga ttaccggaag
     3181 cagaagaaga ggaatgatta actgtttcta agttgatttt ctttttactg gaactataca
     3241 aaaaggaggt gtgacatgaa tgttgtcaca cctccttttt gctaatgggc tgaatgtttc
     3301 ttttactgcc tctcgataaa cggtgttctc ctaccaaccc ctcggtcaca agtcacactc
     3361 tcttttaaat atcttttggc aattgttcat aaaatgctgc ttttgttgcg gaaatgtatg
     3421 ggctgagcca tagataacca ataccagcag ttgctaatgc taagatatgc cagccgataa
     3481 atgaaagatc taaccagaaa agttgtcctt tataaccatc catcaatttt cgacttgcag
     3541 tgatcgtatc cagtacttta ggtgtttgtc ctgtttcttg gacgatatcg tagtagataa
     3601 aatagctttg agaataagaa tagcttttaa tgattccagg aataactaat agcaaacccc
     3661 ataatgttgt aaagatcgag atcagcacag ctaagccgat cactccgccg ataaacaaac
     3721 ctttgaatcc acgaaatgca tctttgaatg gttcgatctg atctttttgt ccacgtaaaa
     3781 tatcaagata agtccaagaa ataccgctca tgaacaaagc gctaatgatg ctggaaataa
     3841 ttccgccacc gcctcctgaa ctaccgcctc ctgaagttgt cgcatctgag acgctcgctg
     3901 atccatcata tatggtggtg gcaaataaga taataggaat cataataaga agaatagcag
     3961 caataccaat caatgtaggg attagattta gtaaaacagc ttgtccccaa cgtcctttta
     4021 gactctcttt tgcttggcgt tttaattctg cagaagattg catgattttt ccctcctttt
     4081 ctttaataac accaatatac catgtatcaa aaaatatttc tgtcataatg tttttctctt
     4141 ttttgttatt
//
LOCUS       DACEUZ010000158         4139 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.158, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000158 DACEUZ010000000
VERSION     DACEUZ010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4139
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.158"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(93..1055)
                     /locus_tag="IXG44_002415"
     CDS             complement(93..1055)
                     /locus_tag="IXG44_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018375965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1659109.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1057..2496)
                     /locus_tag="IXG44_002416"
     CDS             complement(1057..2496)
                     /locus_tag="IXG44_002416"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HAQ1659110.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2681..>4139
                     /locus_tag="IXG44_002417"
     CDS             2681..>4139
                     /locus_tag="IXG44_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sucrose transporter subunit IIBC"
                     /protein_id="HAQ1659111.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDP"
BASE COUNT         1289 a          720 c          673 g         1457 t
ORIGIN      
        1 gttgacaact cctttttgtt tacaaatcaa ttggatatct acaaattgta ttttctaacg
       61 aattttatag acattaaaaa attaatttaa cgttaaatac ttcctccaga aagtattgag
      121 attggtaaaa tataatcctt atttgtcttt tcgcctttaa tttttttcag aagaatatca
      181 acaataagac ttgcaatttc atcaattggt tgtcttattg ttgtcaattg tgggaaaaat
      241 ttctcaataa aagaggtacc atcgtatcca ataattttta aatcttctgg gatattaagt
      301 ttaagttgat gagcaatttt catagtgaga atggctgtta aatcatcaga aacaaaaata
      361 ccatctggtt tgtgatttat aatagttgac tttatttcca tttcacgacg gatagtagac
      421 agattattag gaattttgaa aatttttcca tttggatttt gaaaagaaaa acctagtgca
      481 cgtaattctg tgggagaatc cgtgttatca tttcctgtaa tcataatggt attttgacaa
      541 ccattttttt gtaaagtctt agctgcaagt tttccccctt caaagttatc tgaagaaaca
      601 atggaaacat taggagctaa atttctatca aaagcaacaa taggtgctct cacacgctca
      661 taatcagcaa ttccaagatt atggcttgat gagattatgc catcaacttg gttggcttca
      721 agcatttcaa tgtattctct ctctttagca ggatcatttt gactattgca aataatcgtt
      781 ttatatccat gtttaaataa ctctatttcg agatactcaa tgagttcaga gtaaaagata
      841 ttactaatat taggaaaaat aagaccaata agctgaggtg actttccttg aagtccacgt
      901 gctaaattat ttggtttata accaagagtt ttcatagctt catgaacttt ttgttttgtt
      961 ttttctgata aatatccctt attattaatg acgcgtgaaa cggttgttgg gctaactcca
     1021 gctaattcag cgacatcagt taattttgct accattttaa tattttaatt caaagtattt
     1081 tccagtaggt tcgcctgatt tgatttgaat accattttgg tgattttcag ggaatacacg
     1141 actagtaaac actttttctc ctttattaat aaaaatctca atgattgagt tatctacaaa
     1201 aatgttagca gttgtttctt tagtatcaat tgagcattca cgaatcgtac caaagtcagt
     1261 ggcatattga atgcctgctt tgctgcgatc taaaataatt tttccttctt tggtatctac
     1321 aatcaagcta agtccatttc ctttactatc agagaaaaga agtaactccg atttctgatt
     1381 tggaggaaaa gttagttcaa gctcataagt attatttgtt tctgcttttg ctgtaaattg
     1441 ttcagaacta gcacgtaatg atgtaatagc ttctactgga tattggtaaa gtttaccatc
     1501 ttttattgaa agttctttta caagactcat tgctccctga taatcatact tatcagttgg
     1561 ataatcgata tcaggtagtc caatccaact gacaatcaga gcacgaccat caggagtatt
     1621 gaatccttga gtcgcatacg cttcaaatcc ataatcaaga ttttgaattt ctgaagcgcc
     1681 cacaagtttt actttttcta tgtcaagcga ttgacagatc ttaaaagtat tcggatagat
     1741 attcccataa tttagctcag atttatccaa cccttgagga caataaatga gaacaggttt
     1801 tttgtctaca aatactagat ttggacactc aatcatgtat tctgaaccag tgccgccaaa
     1861 atcaaggttg ccaacttcct cccatgtttc cacgttattg tcatccgctt tgtaaagttt
     1921 gataaagcct gatttttcta aattttgtgc tccaacaatc gcatacaatt gtccttcata
     1981 attaaacatt tgtggatcac gaaaatgatc agttacatca gcaggtttat gaataagaac
     2041 attctcacct ttcgtaatgt tgtagtcttt atccatccat gctccgattt gaagtgtatc
     2101 acgttcccaa ttttcatttc gaacgtttcc tgtatagagt aagaatagat tcccctctat
     2161 ttcatatgca gaaccggaat aagcgccgtg gctatctcgt tttgtgtctg gaagaagttc
     2221 tgctcctgtt acatgaaaat gaactaaatc atctgattca ttgtgtaccc attctttcaa
     2281 cccatgagca gctccaaatg gccaattttg ataaaaaagc gtatattttc cgttaaaaaa
     2341 agaaaaacca ttaggatcgt ttaacaatcc tgttttaggt tcgatatgat agctagcacg
     2401 ccaaggtgat cgttttacat tatccttaat tttatccatc tcatctttag tccaatcaga
     2461 ataagcacga tatcgtattt ctcgaggtaa gttcatattt tactccttca atttatcttt
     2521 ttacaactag tatggtaaac gttttccgtt gaaaaatcaa tcatttcatt taaaaaaata
     2581 aactatatga caaacgtttg acatattttt aagatatgct aaaataaatt tgcatttcag
     2641 tgaaccgttt tcacgtaaaa ataaaaaagg agtgattcga atgaattaca gtaaagtagc
     2701 ttccgacgta atacaagcgg tcggaaaaga taaccttatt gcagctgcac actgcgctac
     2761 acgtcttcgt ctcgtactaa aagatgacac gaaagtgaat caaaaagctt tagacgaaaa
     2821 tccagatgtg aaaagcacat ttaaaattga tggacaatat caagtgatta tcggtgcagg
     2881 tgatgttaat ttcgtttatg acgaacttat caaaaaaact ggactttcgg aaatttcaac
     2941 agatgattta aaacaaattg ctagtaaaga taaaaaattc aaccccatta tggcattaat
     3001 taaattatta tcagatattt ttgttccaat tatacctgcc cttgttgcag gtggtttatt
     3061 aatggcatta caaaactttt taacagctgc tggacttttt ggaccaaaat caattgaaga
     3121 aatgtatcct gcgattgaag gtatttcctc aatgatccaa ctgatgtccg ctgcaccatt
     3181 catgttctta ccaattttag taggtatttc agctgctaaa cgttttggag caaaccaatt
     3241 tcttggtgct gcaattggta tgatcatgac aacaccagat cttggtggct caactgaata
     3301 ttggaatatt tttggttatc atgtttcaca aacaaactat gcttatcaag ttattccagt
     3361 tttagcagct gtttggatat tatctatatt agaaaaattc tttcataaaa aattgccatc
     3421 atctattgac tttacattca cgccattatt atcagtaatg attactggat ttttgacttt
     3481 cacagttatt ggccctgtaa tgttattgtt gtctaatgga attacagaca ttattgtttg
     3541 gctatataac actactggat ttattggaat gggtattttt ggaggaacat attcacttat
     3601 agtcatgact ggtttacatc aatcatttcc agctattgaa actcaattac tttcagcgtg
     3661 gacaaatggt atcggacatg gagactttat ctttgtagtt gcatctatgg caaacgttgc
     3721 tcaaggtgct gcaacatttg ccatttggtt cttaactaaa aattctaaga caaaaagtct
     3781 tgcttcctct gcgggtgtct cagcacttct tggaattaca gagcctgcct tgttcggagt
     3841 taacttaaaa taccgctttc cattcttttg tgctttaatt ggttcaggaa ttgctacagc
     3901 agtagcagga ttacttaaag tagttgctgt ttcacttggt tcagcaggtt tcttgggatt
     3961 tctttcaatt aatgcaacat ctattccatt ttacgtattg tgtgaattga ttagctttgt
     4021 agttgctttt gcaattactt acttctacgg taaaacaaaa gctgctggta tctttaaagc
     4081 agaagaaatt gctgaacaaa caccagttaa ttcttcaaaa gtagcaacaa cagatccaa
//
LOCUS       DACEUZ010000159         4115 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.159, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000159 DACEUZ010000000
VERSION     DACEUZ010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4115
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.159"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            55..507
                     /locus_tag="IXG44_002418"
     CDS             55..507
                     /locus_tag="IXG44_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1659112.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            521..1210
                     /locus_tag="IXG44_002419"
     CDS             521..1210
                     /locus_tag="IXG44_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1659113.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1335..3686
                     /locus_tag="IXG44_002420"
     CDS             1335..3686
                     /locus_tag="IXG44_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HAQ1659114.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            3766..>4115
                     /locus_tag="IXG44_002421"
     CDS             3766..>4115
                     /locus_tag="IXG44_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1659115.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKR"
BASE COUNT         1434 a          524 c          840 g         1317 t
ORIGIN      
        1 ttatcttatc gtagatatat attataaatt taataagaaa gcgaggtgag aatcgtgaaa
       61 aaaacgatac taagtatttt ttcagtattt acaatattaa ttgggggcta ttttcttttg
      121 aatttagatt ctgttaattc aaacgctcaa ataaaagatc aaagagtagc tgaacataat
      181 aataaaaaaa tgagtttgac caaaaatatc tattctatgt cagatgaaga attgtataaa
      241 gaatttggtg atttatccgg ggaacagtta gaagaaagta ttccaggaat tagtgactca
      301 gataaaattg ttgtcttgtc tgctggtggg caattaatgg gagatggcaa atccgaatta
      361 gttagttata aacaaaatca cagacagttt attgatgtta atactgattc caacgctatt
      421 tcgattaagg atctaaaaga gcttttaaat agtaaaagaa atgaattaaa gcagataaaa
      481 gaaactaatt cagtggaatt agagtaaagg aagattcatt atgaaaaaga tttttggtgt
      541 ttctgttggg ctacttatga tgttaacatt tggaaatgca gtatatgctg ctgagaaaga
      601 agttatttca gatccagagc agtacgcctc acgatacttt gctgattttg aagaagaaga
      661 taaagtactt gcattgccag aaggtggttt tttaaaaggg gaagctaaaa ttgtatctat
      721 ggataatcca gatataattc aaggttcata taattcagaa actgatcctg cttcagtttc
      781 agtgacagaa gcaaaaaaaa ttgttgctga aaaattcaaa aatgatattg aaactactcc
      841 aattatgcct cgaggagcat ttccacccga tgttggtact agcggacacc ttcttgtact
      901 agcaggagga ggaagctata aatcacagcc atttagtggt gcgggatggc gttttggagg
      961 tatgggtgta aaaccagctg atggtacagg caacttttta cgttggaaat cctacattga
     1021 cgatggaaga gtaggaaatg taaatcaagc caacgcaact aaatctggtt ctattcaagg
     1081 aatggcattg tatgctcgtg aaggattcaa atactttgca ccaaacgggc ctggttggac
     1141 aatgaatata ggtatttatt acacttataa tcctgttgca ggtacgtatt atgttgttca
     1201 aaatatttaa taaaacagca caaagagatt tctttgtgct gttttattat ttttagttgt
     1261 tgacaaacac taaaatataa aatataatca acacataaac gaacgtatgt tcggcaagaa
     1321 agcaggggat ttttatggat gagatggttt taagtactca gaaatggcta aacaagaaat
     1381 acagtaatgt tacagggttt gataaggttc cagaaaatgg gcgaacaggg tggcctacaa
     1441 tctatggact tattgaagga ctacaagtag aacttggtat tactaatcta gtagctaatt
     1501 ttggacctac cactgaaaaa atgtacgata atcaggttac tcctaaatgg ggcaaaaatc
     1561 tacctaaaaa tattgtattt cttattcaag gtgccttttg gtgtaagggg attaatccag
     1621 gtggcttcga tggggtatat actccatatt tagatactgc tgtaaaagag ttgcagactg
     1681 atgcagggtt taatggaagc gctgtaactg gagttttgga tgcgaaatgg gccaaagcgt
     1741 tatttgatat gagtgctttt gttcttgttc ccggcggaga tagtaaaatt agaaaaatgc
     1801 agcaatggtt aaatgtaaat tatttagaat acacgggaat aatgccttgt gatggtattt
     1861 atcaaagaaa ttcaaatcaa gcattaattt ttgcattaca agctgaacta ggatattctc
     1921 ccagtgaagc aacagggtct tatggtaatg ggacaactag taaaacttat cctgtgtctg
     1981 aaggtaactc gggaaactat gtaagaataa ttcaatatgg attatatgtt aatggttatt
     2041 ttgaagacgg tacattcgat ggtattttca ctaaatatat gggtttagaa gtactggcat
     2101 tccgaaaatt tatggtattg cctgaatata ctgaaatagc agatgttgtt gtaataaaag
     2161 gactattgtc tagcgctgga gatattagaa gatctgcaga tggcgcagat acttctacgc
     2221 aattaactag atctcaaatc caaacattgg ttgataatga tataaaaata gttggtagat
     2281 atcttactgg tactgttgga attggaaaag atgaacgaaa taagtatcta actacagagg
     2341 aattgaataa tattttttct aaaaatttat ctgtgtttcc tatctatcaa gatggaggtg
     2401 cagcattagc ttattttact tatgatcgtg gcttatcaga tggtaagaag gctatagatg
     2461 cagcaaaaaa tctaaatata ccattaacaa cagttattta ttttgctgtt gatctagaca
     2521 tgttagggga agaaattctt gcatatgctg gagaatattt taagggggta tctgctgtaa
     2581 tgtcatatag cgggtatcaa acgggtgttt acggaacaag aaatgtttgt tctcaaataa
     2641 tcaataacgg gtatgcttcg tttgcatttg tttcagatat gtcgactggt tatagtggga
     2701 atttaggttt tcctatgcct agacaatggt cttttgatca atttgttgaa tttaccatag
     2761 gaagtggcaa cggagctgtt ggtatcgatt taatcgctac atcgggtaga gatagcggat
     2821 ttaatgagtt atcaaatgat actaataatg ataattacat tgccaaatac aatcagaagg
     2881 ttataaatca gatggtacgt gcgtatcaat atctatcaca agctggtctt gataatcctt
     2941 ggaaccctca tttgacattt tatagatatg taaattattc tggtttgtca tgggacatca
     3001 tttcttctcc tgtaacagag catgatagaa aaatttatga tgaatatagg aataaattaa
     3061 ctaatagcga gggcctgtat aattatttta tagatcctaa tactggttca ataataggtt
     3121 tgcctcactt aattgtgacg ttacaatcac agatgtttct agccgacaca atcaatgata
     3181 gtgtttctga ctttgcaggt tggttaggag atttaatgac ttgctggggt gaagttaaga
     3241 aattaggaat acaaatggag caaggtgtat ttgcattagt aggtacggct gcaggtggaa
     3301 catttagtct agaagattta taccaagatg ttgacgcggt aaatgtttat accttagcaa
     3361 aaagaaattc tgattcaaca gctttatcgg ctttaaaaac gtactattgt gataagggtg
     3421 ctattgatag atttaatcaa tttgttaaga acagatttgg ttcttcagat aaaatcgctt
     3481 cagtaacaaa tagtttatta gaaaatgacg aaacagttga tatcttaggt gtagggataa
     3541 tttcattttt cattgataaa atagttaata attcacttgg gacagatttt acttatcttg
     3601 attttattgg agatgagggg aaagttgcaa atggattttc tgaaaaaatt attcaatttc
     3661 aagaaaatag aagtaaactt aattaagaat cgtttactat ttaaacagtg tccttttcct
     3721 ttttaaatat atacgataaa ataagtatat gaattgtaaa aggacatgat gattatggaa
     3781 gaaatagaat ggaataatct gactcaaaat aataaaaata attctggatg tggaattatc
     3841 tttatgtttg ttatagtttt gggaatagta gtacttttaa taactgtcaa gatagtagat
     3901 tactttgcga atactcctga agaacaaata cagagaaaac ttcctgagtt tgaaagagta
     3961 ccaccttatg atccaagaga taattcattt caagtaaaaa aattaaaaga gtctccaaat
     4021 gaaatgtggt ttgaagttac agttgaatca agcgacttca aagaaaaaaa gaaagaatac
     4081 aattatattg taaaggcaaa agtatcaaaa agatt
//
LOCUS       DACEUZ010000160         4015 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.160, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000160 DACEUZ010000000
VERSION     DACEUZ010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4015
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.160"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(176..1462)
                     /locus_tag="IXG44_002422"
     CDS             complement(176..1462)
                     /locus_tag="IXG44_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAQ1659116.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(1455..2723)
                     /locus_tag="IXG44_002423"
     CDS             complement(1455..2723)
                     /locus_tag="IXG44_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAQ1659117.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYPDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            2975..3436
                     /gene="mscL"
                     /locus_tag="IXG44_002424"
     CDS             2975..3436
                     /gene="mscL"
                     /locus_tag="IXG44_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HAQ1659118.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(3495..3941)
                     /locus_tag="IXG44_002425"
     CDS             complement(3495..3941)
                     /locus_tag="IXG44_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1659119.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGQ
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
BASE COUNT         1137 a          816 c          707 g         1355 t
ORIGIN      
        1 gattacttcg tttgatttta ttttaatata agttgattaa aagagagcat gatttgtttt
       61 attttctcac taatttttcc aacacaacta agaataccat aaaagctgat tttggaggag
      121 gttcctacta caagtctaaa acgaaattta agacttttcc tcataaaata ctggtttatt
      181 ttggtctcat gtaaaactcg ctgaaggcct cttcacttat aaatgcttcc ccagctgcca
      241 gtacgtcttt catttggatc gaatcgatga tttcaggaag atcaaataaa gttcgatcgc
      301 caaataaact ttgcgtaaat tgattggcaa tatattcgat cgaatttaat gattggaaat
      361 attgtccaag cattttcttc ttcagcaaat cgagatttgt ttctgacact tccggatcat
      421 cagcgaatcc aagaatgatt tctttgactt tttctgctgc tttctctggt tcgtccgtat
      481 ctccgctgaa gtccgcaaag tgaaattcac gatccaagct gaattcaaaa ccaaagctgt
      541 catcgataat tccttgattg tacatcgcta gatagtttcg agaagtattg cctaaaatca
      601 tttggaacaa cagattgatt gctgttttat agcgtaacag ttctgttcct tcttgcggca
      661 atgtgtctaa tcctttaata ccaagtacaa atttatctcg tgtaatggct gcttccaatg
      721 cactttgttt gatgatttct tttgtgtttt cagggaaata acgtacaatt tcttgctttg
      781 gagggaaatt ttttgcttct tggttttccc gaataagttt catcaatttc tcaggctcca
      841 tcttaccaac tacaaataaa accatattgc ttggctgata aaacgtccga tagcacgtat
      901 acaagtcttg agctgtgatt ttgtcgatac tttcaaccgt gcccgcaata tcgatatgca
      961 atgggtgtgt aggatacaag ttatttagta tgccaaaaaa catccgccag ttagggtcgt
     1021 cttcatacat ttggatttcc tgaccgatga ttcctttttc cttgtttaca gtttcttctg
     1081 tgaagtaagg tgcttgtaca aaatcgatca gagtcgtcag attcttttct acctgatccg
     1141 tcgtagaaaa cagatagctg gttttcgtaa aacttgtgaa agcattggcg gaagctcctt
     1201 gtttcccaaa taattgaaag acatcaccat cttctttttc aaacaactta tgttctaaaa
     1261 aatgggcaat tccatctgga actttcacct tttctttttc accataggga ataaattcgt
     1321 tatcgatcga accataattc gtactgaaca aaccatatgt cttgtgatag tcatttttag
     1381 gcaacaaata cacggtcaaa ccgtttggca gtacttcatg ataaagtgtt tcattgattt
     1441 gctcatattc tgttttattc atttgcattt cctcctgcta aaaagaagat tgcctgcaat
     1501 tcgatttgct cagctacttg ttgtatctct ttgatcgtta cgctctccat acgtttcaac
     1561 cattcttcat cagataattt tgtttccggc agccagctgt ccaaaaaaga agcttcgatt
     1621 gccgcttgcg gagagtctag agaaagcaga tattggtttc tcagcattgc ttttgtctga
     1681 gacagttcca actctgttat ttcaccattt cgcaaacttt ctaattgctc gtgaatcaga
     1741 tgaagtactt gatttcggtt cttttcatcg ataccggttt ggacactcat gaatcctcgg
     1801 aacgtatcca cgctactaga ggcataataa gctagacttt ctttctctct gacattcatg
     1861 aaaagtttag agtgaggaaa accgccaaat aaaccgttaa agaccattag cgcaaaacgt
     1921 tctggttcat cgtaatacac atttgtctga taagctaggt tcagctttgc ttgaacgatt
     1981 ggctcacgta cttgtcgttc cttcacgata ttcacttgag gttgggtata aaacagatct
     2041 ggataaactg tttcccgcga ctcaaatggt aattgcccaa ctgcttcagc tgctttgtcc
     2101 ggatcaacat cgccgacaac aaaaatgtcg atttgatcat ttgccatcat ttcttgatag
     2161 taggacgcta gcgaactagc agtgatcttg tccaaatctt cgactacgcc caaccctggg
     2221 atcttttgtt ccgggctgtt ttcaaaataa agttcttgca aggcatagga agcaaaagtt
     2281 tgtttatctt ccttcaaact ttccaaatat agacgcagat tgttcttttc caagtcaaac
     2341 gtcaactgat caaactgatg atttttaata tttggataaa ataaaacctc ttttaaaaaa
     2401 tctaccgctt gagaaaaaag gtttggatca ttgacatatt caccattcac aaaactgatc
     2461 cctgcgttta gccaatgtaa atttcctttt tttctaactg ataatccaaa gcttgcgcca
     2521 taaagctctg caagttgcgc acttaacttt gtctgatccg gataattcaa actatttgtt
     2581 tccaacatgc tgctgagaag tgaacgtttc gtgatgactt cttgttgcag acgcgcagta
     2641 aaacggataa aaatccggat cgttttgtat tgttttgttg gtaagacatg aagattcacg
     2701 ccttctgcca attgatacac catatttata tgctcctttt cacagttgat tgcccgctta
     2761 ctcttttaat gggtcaacct gcctttttta cttattcctc tgcttgaaat ttataagttc
     2821 aggcaattcg ccgttttttc taaatgatgt tacactttat agtatagcat agatgttttt
     2881 tatcaaaaga aacagacggc taaaagcgta tattgttttt ttatttttta tttttcgata
     2941 tactaaagac aaataaaaga agggagggtc attcatgatc aaagaattca aagagttcat
     3001 catgcgtgga gatgtcttgg atcttgctgt cggcgttgtt attggtagtg cgtttacaag
     3061 catcgtaaac caagtggtgc aaggcttgat cacaccacta ataggttggt ttgtcgcaat
     3121 gatcacaggt acttctgatt tagaaggagc actttcaatt cttgactggt cgccgacaaa
     3181 aggcgtcaca tttgcgtttg gtaacgttat tagtgctatc attacatttc tgatcactgg
     3241 ttttgtctta ttcttagttg tcaaagcggc aaaccgtgcg aagaatttac gctccaaaga
     3301 agagcaagtg gaagtggaag aaactcctac tgcagaagat tatcttagtg acatacgcga
     3361 tcttcttgcc aaacaagtgg cagaagacaa tacacatcca gtagagcaaa caaatctttc
     3421 cgatacttct aaataataaa gaatctaaaa aaaagaaagc cgattttcag tgtcggcttt
     3481 ctttttttag attcttaact tgctacttgt tcctttttca tgtcgatatg tggaatatca
     3541 tcttccaaat acacatctga gattgccttg aatccaaacg actcataaaa agcttgtagg
     3601 tattcttgtg cgccgatcac gattggtttt tctggaaaat ttttctggat catgttaatc
     3661 gtttcttgaa caatctttct tcctaaacca tctttacggt tgtcctgatg aacaaggaca
     3721 cgtccaaaat ggatactctc cgcttcttca tatactcgcg tgtaggcaag gatcttccca
     3781 tcagtatctt gaaaaaacgt atgccaagct tgtccatcaa tgtcatcaac ttcttgataa
     3841 ggacactgtt gttccacaac aaaaactgcg attcgttctt tgacgatcgc gtaaaactct
     3901 gctgttgaca attcttcaaa tttctttacc tgatattcca ctaagcaaac gccccctgct
     3961 tttttattct tagcataccg attcgcacac aaaaaagcaa acgaacctta cgaat
//
LOCUS       DACEUZ010000161         3993 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.161, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000161 DACEUZ010000000
VERSION     DACEUZ010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3993
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.161"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(113..388)
                     /locus_tag="IXG44_002426"
     CDS             complement(113..388)
                     /locus_tag="IXG44_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HAQ1659120.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(360..1313)
                     /locus_tag="IXG44_002427"
     CDS             complement(360..1313)
                     /locus_tag="IXG44_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ1659121.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            1925..3418
                     /locus_tag="IXG44_002428"
     CDS             1925..3418
                     /locus_tag="IXG44_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepR"
                     /protein_id="HAQ1659122.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            3553..3849
                     /locus_tag="IXG44_002429"
     CDS             3553..3849
                     /locus_tag="IXG44_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAQ1659123.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            complement(3873..>3993)
                     /locus_tag="IXG44_002430"
     CDS             complement(3873..>3993)
                     /locus_tag="IXG44_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ1659124.1"
                     /translation="FIQSVASKRNNIPRKSLNYRTPLEVFLSYVSIDDLSNLI"
BASE COUNT         1328 a          653 c          717 g         1295 t
ORIGIN      
        1 atgttgtata cccccaaaca atacacatat tttgaaggat taggattaat aaagctaaaa
       61 atatagtaag tatactaaat atatttacta tatctagtat acttactata ttttaatctt
      121 ttgatcgtct attatatata atccgttcct cgtcgtttaa agtatcaaca taggcgtcca
      181 ttgcaagttt gactatttca tacataggaa tatcttttgc ataactaaga tcccgaatgg
      241 tttttagtac gggaggatct acttgaactg gttttctttc tttcgcagtg tactttcgtt
      301 cccctaactc gacattaata tcatctttaa ttgaaatatc ttcagcacca attttcttct
      361 tatctttgtt cgttggtaat aatgccattt agtttaatct cctttccaag aggtaggata
      421 tgattggcca ttgagtcagc atatttaggt gtataatcaa atccttcaat atcaccatgt
      481 tgttcatagt attcaattct ttcttctaat tctgtaaaga tgtctgcaaa aatagcccac
      541 catcttcgat ctacataatc atttaattgg ataccattct caccatacat ttctaaccga
      601 tccgagcctt taacaatgct tttaaaaatg tttgattcac catacttatc aattgttttt
      661 tgatagtttg cgtgttgaga ggaacgtcgc actgagaaaa gaacaggcaa aattccaatg
      721 atttgaagat gagactcttc aaaattcgtg acaatcggaa ctaaaacttt attaataaaa
      781 tcttctgttc cttccattgc gtaccgtttt aattcttgca ttgcaataat ataatctgca
      841 gcagttaaga atgcacgaac cacattatct gttgatggtg ggcagtcgat aaagatataa
      901 tcgaaatttt ttctcaattc ttctaaaggt ttaatgaagg ccaatgatct cttacgacga
      961 tcaggatatt tttcggacaa gtattcatta agttcttcag atccaggaga gccagccata
     1021 atatataaat gtggacttac ttgagtaata cctgaaaccc agtccccttt tttgattgct
     1081 gcagacatgg atcgatcaaa gctagtaatt tgatacgttg atccaatgtt ggaagtagta
     1141 tttctttgtt ggtcataatc aattagcaac acgtcttttt caaaaagttg actagaaaca
     1201 atcgctaata aatcattgtc tgtagtctta ccaatgccgc cttttctacc tgtaagcatt
     1261 acaacttttc ctagtttatc tgatgtttta aatgcagtcc gtaagttttc catagttccc
     1321 tccaatttcc tatactaaat atatttagta tacttcatat acttaatata ttcgatatat
     1381 ctaatatact ccaaattgat aagaattcag actttataag gaatatagtg gatatactaa
     1441 atatatttgc tatattcagt atatcagata tattcaaaat gtcaaaatat atttgtgcgt
     1501 tttgcgttga caatttagag tgaaaagagg taaacttaat tctatagaaa ccacagataa
     1561 ttcactattt acaaagtagc aaaagcagtg gtactcttta aacagaaaaa ttacataaaa
     1621 aaaagagacg gaaataattg attttagcag aatcaattat ttctgaatca catggttagg
     1681 acatgtgttc acgctcgtct caagaatatg taagtagcta ccaactactt atattaaaag
     1741 ttgatcggat accttttccg attgccttta ttctaccaaa aaaaggcgag caactcaaca
     1801 gaagaacgtt tatttagtgt acttaaatta aatattctta aacactgaag agagaacaca
     1861 tactatggct gagattaaag ctaaactgtg ttctcttttt gtttgagaaa ggacgttaca
     1921 atcgatgttg agcgaaattt attcagctat tcttaaaaac ggcttacgta catacaaata
     1981 tcgaaacagc accatgaaac ctgcaggtca tattgattcc agtaggagtg gtgctatctt
     2041 tgcgtaccgt agcaagcaat taatgaatgt tgggcgtggt atggtcatta cttcggaaga
     2101 agctatttta gagaacgaaa agaaactaac gcattggaca cctaatacct atcgctttgg
     2161 tacttatgcg gatgattatc gaacagtggt taaaggtcat tctgagaaaa acttaagtca
     2221 aatcaacaca tttgtagttg atatcgattc aaaagagaac catcaaggcg aaattatttt
     2281 agcctgttta gatcaagtgg gctacatgcc aacgctaatt ttagaatcag atcatggtta
     2341 tcaagtttat tttgtactaa agacaccagg ctatgtcaca aagaaaagca atttcaaggt
     2401 gatcgatgta gcaaaaaaaa tttcaaaaac aattcgtcta cagttggctg aaaaattgtc
     2461 cggagtggat ctaggatgta accacttcgg aattgctcga ttcccacaag aaaataatat
     2521 tgtttttaaa gaaatgaact atcagtattc ttttgctgac tggattcaat ggtcgatgaa
     2581 gcttgaatca gatcaaaaaa caaaagaaga tcgagaacga aaactaattg tctttcctga
     2641 gaaaaaagaa attcgtcaag tagatgaacc gtggttcgat atgttacttc ataaagcaaa
     2701 catcgtcggc ggagaaggac gtcttggtcg aaataatgtg attttcacgc tttctttagc
     2761 ttattactct tcaggttatg cattggatac ttgcgaatac aatatgttca cattcaacga
     2821 acggttacag gaaccgctat ctgaagcaga attagtaaag attgtacgta gtgcttattc
     2881 tggtaattat caagcagcaa gcagagaatt cattattgaa ttgtgccagc aatgggtttc
     2941 accaacctta aaaaataatg atctgttcat tcaacgacgt ggctggtgga aatttaaaaa
     3001 gccacgagat caaagaaaat atagtcataa aaatgaatgg aaagaagatc tattggctta
     3061 tctaagcgaa aaaagtcttg ctagtgagcc ttatttgaac ctgtcaaaaa aagaattggc
     3121 ttcacaacta acgatgccca agcgaacact tgataagatc ttaaatgagt tacgagagga
     3181 gaataaagtc ttctacaagg taacacgagg aagaaatggc ggaatgatca ttgcttcagt
     3241 caaaacgttg ttcgcgaggg ttattcaact aagtaaagaa aagagactag attattcaac
     3301 aaggatcaca gaggtatttg gactcaacga gagcttagta gaagagacat ttagacaatt
     3361 atcaaccccc ttgaaccctc ttaaagaagt gaatcttttt gaagtagata caggttgaaa
     3421 aataaaaggt gcaaattgcc ttatacaata tatctatgat acttctgtag gtgttgctcc
     3481 ctttgcctag ctacgtgtag gtggtatact tataactgcg tatgcagtct tatgtatcat
     3541 ggaggaagtt gtatgtcgtt aaaaaattat gtctattcac atcctgttaa tgtgtttatc
     3601 attggtcgtt tgggattatc tgtggaagtg ttctgtgagc tttatggttt taagcagggg
     3661 actttgtctt cttgggttac cagggaaaaa acagttgctt ctttgccgat tgagtttctt
     3721 catgggttgt ccttagcttc tggggagact atggatcagg tatatgattg tttgtgtgtg
     3781 ttagagcagg agtatattga gtataaaata gctaacgagt taagaaaaag gaagaagtat
     3841 atccagtagt agtttttatt tactcttaat tgtcaaatta agttagacaa atcatcaata
     3901 cttacgtaac tcaaaaatac ttccaaaggt gttcgataat tcagtgattt tctaggaata
     3961 ttatttcgtt tcgatgcgac agattgaata aaa
//
LOCUS       DACEUZ010000162         3956 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.162, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000162 DACEUZ010000000
VERSION     DACEUZ010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3956
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.162"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            <1..156
                     /locus_tag="IXG44_002431"
     CDS             <1..156
                     /locus_tag="IXG44_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HAQ1659125.1"
                     /translation="DKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPVDA
                     ISYGVRK"
     gene            175..1452
                     /locus_tag="IXG44_002432"
     CDS             175..1452
                     /locus_tag="IXG44_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1659126.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            1462..2118
                     /locus_tag="IXG44_002433"
     CDS             1462..2118
                     /locus_tag="IXG44_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1659127.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            2118..3494
                     /locus_tag="IXG44_002434"
     CDS             2118..3494
                     /locus_tag="IXG44_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1659128.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGGVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            3624..>3956
                     /locus_tag="IXG44_002435"
     CDS             3624..>3956
                     /locus_tag="IXG44_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ1659129.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDI"
BASE COUNT         1594 a          425 c          694 g         1243 t
ORIGIN      
        1 gataaggata agtgtattaa ttgtggaaaa tgtaaaaaag tttgccaaat gaataaagat
       61 tcagttgaga attgcaatca tctggagtgt atcagatgtg gacgatgtaa aaatgcttgt
      121 ccagtggatg caattagtta tggagtaaga aaatagaaag gagtagttag gaaagtgata
      181 aaaaatgcta tagcatatat tacaagaaag aaaaatagaa ctttaataat ttttatcata
      241 ttaactatag ttctttcttg cttgtattct tgtttaacaa taatgaaatc aagcaatgaa
      301 atagaaaagg ctttatatga aagttctaat tcttcaatat caataacaaa aaaagatggt
      361 aaatatttta atgttaatca atttaaagat attgaaaaat taaaagaaat tgaagaaaaa
      421 ataattcaat atgatggatt agcaaaacta aaagatgcta aagtagttag cggagaacaa
      481 agaataaata gagaagattt atctgacgaa tttaagaatg ttgtttcact cgaagctaca
      541 aataatacta aaagaaatat tttatttagt agtggagtat ttacaattaa agaaggtaaa
      601 aatatagaag aaaatgataa gaattcaatt attgttcatg aagaatttgc taaacaaaac
      661 aatctaaaat taggtgatga agttgatctt gaattactag atattgaaaa aagtggaaaa
      721 ataaaaagtc ataaatttaa aattataggg atcttttctg gtaaaaaaca ggaaacatat
      781 acaggattat cttctgattt tagtgaaaat atggtgtttg tagattattc aacaagccaa
      841 gagatattaa acaattcaga aaataatgaa attgcaaata aaattttaat gtattctggt
      901 agtgcagaat ctacagactt agccttaaac aaattgaaag agcttaaaat tgatgagtca
      961 aagtattttg ttgaaaaaga ttctaatgcg tttgaagagt ctttagagtc agtgagtgga
     1021 ataaaatata taataaaagt aatgacttat tctattatgt tgggtgggat ggttgttctt
     1081 tcattaatct tgattctatg gttaagggaa agaatttatg aaataggtat attcttatct
     1141 attggaacat ctaagataca aattataatg caatttatat tcgagttaat attcatatcg
     1201 ataccaagta taatatcttc cttattttta ggaaatgtat tactaaaagt aattgtagat
     1261 ggattcatta actcagagga ttcaatgatt tccggtggaa gtttaataaa taatagtagt
     1321 tttatgtcaa atataacaac acttggacaa agttatttaa tattaataag tattattgtt
     1381 ttatcagttg tatttgcttc ttcattaatg ttaatcaaga agcctaaaga aatcttatca
     1441 aaaataagtt aggagaaaat aatggatata ttagaaataa agaatgtagc atatagttat
     1501 gcaaattcta aagaaaaagt tttgtcagga gtaaatcaaa aatttgaact tgggaagttt
     1561 tatgcgatag taggaaagtc aggaacagga aaatctacac ttctttcctt acttgctgga
     1621 cttgataaac ctcaaacagg aaaaatattg tttaagaatg aagatataca aaataaagga
     1681 tatagtaatc atagaaaaaa taatatatct ttagtatttc aaaactataa tttaatagat
     1741 tatttatcac caattgaaaa tattagatta gtaaataaat cagcagatga aagtatcttg
     1801 tttgaactag gtttagataa aaaacaaata aaaagaaatg ttatgaaatt atctggtgga
     1861 cagcaacaaa gagtagctat tgctagagca ttggtatcag atgctccaat aatactagct
     1921 gatgagccta ctggtaatct agacagtgtt actgctggag aaataattaa tatattaaag
     1981 acattagcta aagatagaaa taaatgtgta atagttgtaa cacatagtaa ggaagtagca
     2041 gattctgcgg atatcatttt agaactaagt ggtaaaaagt tgaaaaaagt aaataaaatg
     2101 aatttggagg ttgaataatg ataaaaaatg catttgctta tgtaactaga aaaagtttaa
     2161 aatcattaat tatcatatta gttattttat ctatgtcaac tttaagtctc attagtttat
     2221 ctattaaaga tgctacggac agagcttcaa aagaaacatt tgctaatata acaaatagtt
     2281 tttctatgga gataaataga caagtaaatc caggaacacc tagaggtggg ggaaatgtaa
     2341 aaggtgaaga tattaaaaag atatctcaaa cagatagtat agactcctat gtaaaaagaa
     2401 taaacagtgt tgcagattta gttgattatg atattattga aactcaagag actcttgcga
     2461 atcaatcacc tgaaagagca aagaatttta aaagaacagt tatgttaact ggggttaacg
     2521 actcaacaaa agaaacaaaa tttgtatcag aagcatataa attagtagaa ggaaagcatt
     2581 tagaaaatga agataaaaat aaaatcttaa tgcataaaga tttagctgaa aaaaataacc
     2641 ttaaagttgg agataagata aaaataaaat ctaatttatt tgatgctgat aatgagaaag
     2701 gtgcagatga aacagtagaa gtagaaatta aaggtctatt cgatgggcat aatagcggtg
     2761 gagtaagtgc agcacaagaa ctatacgaaa atacattaat cactgatatt catacagctg
     2821 ctaaagttta tggtaatacc gaagatacag ctgtatacca agatgcaaca ttctttgtaa
     2881 aaggtgataa gaatcttgat agtgtaataa aagatcttgg gaaattagat ataaattgga
     2941 gagaatataa tttgattaaa agttcatcaa attaccctgc attacaacaa tctatatcag
     3001 gtatttactc aatttcaaat aaattatttt ttggctcatt aatatttgca ggagttgtag
     3061 tttcattatt attattctta tggatgaatg ctagaaagaa agaaatagca gtattactat
     3121 cattaggtat atcaaaatta gaaatttttg gtcaattcct aattgagatg gtatttatat
     3181 caatccccgc attcgttggt tcctatttct tagctcaata tacagctgac aagcttggga
     3241 ataatatatt gaataaggtt actggtgata tagctaaaca aatagctaaa caatctgcat
     3301 ctagccaatt aggtggtggg gcagaagctg aaggatttaa caagacatta tcaagtttag
     3361 atatcaatgt attacctaaa ttcatgattt atgtagttat atttatgagt ttagtacttc
     3421 ttgtatcatt gattatttct tcattcaata tattaagaag gaatccaaaa gaattattaa
     3481 ttgacaataa ttaaaatttt cggtgctttt tagcaccaaa aatgagagtg taatataaac
     3541 cgtgtaagta accatcaatc ctaggcgggt ggttgcttca cggtttttat ttttctaaaa
     3601 taagatcaag aggtgattac acgatggcta gaaagaaaag aaatcctgat gctgagaagt
     3661 tagctgaatc cattttgaat acttatcaac ccgaatctgt agaggatatg caagatgttt
     3721 taaaagatgt gtttggtccc ttatttgaaa aaatgcttca aggagaattg aataaccatt
     3781 tagggtatga cgcccattct aaagagccaa agaaacatga caatcgacga aacggttatg
     3841 gcaataagac gcttaaaacg agctttggta aagtaaacat tgatgttccc agagaccggg
     3901 aggcttcttt tgaacctgag ttgattccta atagaaaacg agatgtctcc gacatc
//
LOCUS       DACEUZ010000163         3925 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.163, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000163 DACEUZ010000000
VERSION     DACEUZ010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3925
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.163"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(573..1130)
                     /locus_tag="IXG44_002436"
     CDS             complement(573..1130)
                     /locus_tag="IXG44_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1659130.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(1309..1920)
                     /locus_tag="IXG44_002437"
     CDS             complement(1309..1920)
                     /locus_tag="IXG44_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1659131.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(2033..2290)
                     /locus_tag="IXG44_002438"
     CDS             complement(2033..2290)
                     /locus_tag="IXG44_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1659132.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            complement(2887..3165)
                     /locus_tag="IXG44_002439"
     CDS             complement(2887..3165)
                     /locus_tag="IXG44_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1659133.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            complement(3199..3450)
                     /locus_tag="IXG44_002440"
     CDS             complement(3199..3450)
                     /locus_tag="IXG44_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1659134.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
BASE COUNT         1195 a          799 c          520 g         1411 t
ORIGIN      
        1 ttattccgaa taaatggtgt ccaaaagctt gcatctgcca gattatctca tttcttatga
       61 caagctaagc aaattatctc caccaaataa tttattcata tttttgactt gcgcatacta
      121 actaattctt aaattttcat acaaaatttc tcaatttccc ttaacgagta atatttatgt
      181 acggtgattc tcactagtat cccaatcact ttttaagtta tcccgatttc aattctactt
      241 caaaaatagt tgcttttatg tatgctgtta gttgattaga tggtcatttg ttgagctagg
      301 tcgtttattt taaacaagtt gtttctttag cttataccta tctcgtctac caaacgcaag
      361 atggcaaaca caaatgcata cgcatctgta tttgccactt tgattcgtat aaagctaaca
      421 actattaact cctctcagct aatagttgct ctctttttta ttcagattca tttttgctga
      481 tcacagccta gtatttcaat ggttttgaac actaaaccaa attgttccat tacaacattc
      541 actggctgat gaaatggatt agtagatttc gattattcaa agcgattttc tattggtgga
      601 tcgtgttccc aactaataag acggattcca aacctatcgt acagatcttc taaaccactt
      661 ataaattttg cacctatttc aggtgtttta ttgcgtggat attctattgt tacgccatta
      721 aatggatacc aattaggatt aggctctctt tttttctgta catctcccat agaatagtta
      781 gcactttctg ggacataata tattcttcga ccatcctcta attcatctaa taagatattt
      841 cgtgatcgtc tatcacttgc taaatgtaga cgccacgtgc gataccatgc catgctacct
      901 gcatctgcag aaaatccatc aaataatgca acaattgtat cttctatcac aatcctttcc
      961 ccatctactt catattctaa tctaaaatca aactcccctc gggtaatttc tggtcttggt
     1021 ggcttaggac ccaatattat tctacttaaa accgcattcg ttcccagatg aaacacagct
     1081 ataacaacaa taatgattaa tattttgttt ctatttgata aatttctcat tattcgcaca
     1141 tcacctctac attctcacat attagttcgt aatactcctt taatatcaaa atatctactg
     1201 gtcgtaataa tattcgctat gaattcctac tactcgtata agaaaggcca aatcatcagc
     1261 ttttacttgc tgatgatttg gcttgtttct atttatcgta attgttttct aatcaccaaa
     1321 attgttaaca atcggttcag atatttccca gttgattaag gtaattccgt attgtgtcaa
     1381 aacttcttgc gcttctccga aattaagtac agttcttatg tatccacttt ctcctaatat
     1441 aaagtctacc tctcttttaa aaccttcctc gagatttcta agggctatat gtggttttaa
     1501 tgacattgat tgtacatttt cccccatata atattgcgca cttcctcgac taaaatagat
     1561 tcctagatcg tccccaaaag gtagatcttc tctaccacta gctaatctac ttctccatct
     1621 tcgttctctt ttcatagaac ctgcgctgaa ctcacacccg ttaaactcag ctatgacagt
     1681 atcttcaatt atgttaatct gtccatctag ttcgtattca atcctaaatg gaaattctcc
     1741 ataggtgatt tccggttcgg gtattttagg tgagattaat gcaaagaaag gaaatattaa
     1801 gcttggcaac atcaaaaaaa ctcctcctat gatcacaatt cccgatgtaa tccaaaatat
     1861 aatttgtaca attctcattg atccattgta ttccatttta ttccccctat tactattcat
     1921 atccgcctat ctatatcgat cttccaatgc agagataatt gcttccatac cttgattttt
     1981 tactgagtta cctatgcgag atatcattct ccactaaggt gcatcatatt gtctatctaa
     2041 catgattaat tctccaatag gtgtatactc ctccaaaaac aacatttaat gcatctccct
     2101 cgacaacata agctaccatg tttgctgtat atgtgtttga gtctaattca taagcagata
     2161 aatttactgt cgctggatta aatataatcg cattcgtatt tatagctact gaatttgctg
     2221 cttcagcacc accttttgaa tgtccgatca ttgtgatttc aatagtagga tttacgtgga
     2281 cagccctcac cgtttaccct gaccattata gaaatcgctt acttttttga actacgcaac
     2341 aaaggataca atgctcctaa cttcactgtc tccatttttg tccactaagg aatataaaaa
     2401 ataggcatta attcatttaa ttcggtcaca acacgttacc ttttaaatca ttatactgta
     2461 tctcatacat tattatcaat aaactattat aaaaatgcaa aaaatgcttt atcaatctat
     2521 tttgtaattg gtcataatat cattgaaatc ataccctgta tcgatttttc ctatgaatct
     2581 cgtctatttg tgattgacga aaaagaggca agacagacac tggaatggta attttaccca
     2641 ctattttgct ccaactattt gtaatcgtac ccacttttga aataccgaga tgatcatttt
     2701 aattgatatg ctgattccca agcaaaaccc caagaaatta aatgctctat cgcatctagc
     2761 ttcttgggtt ttgcttatat aaattccaac atttaaattt tttatgctgt tcggtctcag
     2821 aaatgccgtt tttgcttata tcacaatatc aagatgtcaa attattcgtg ttgccgatga
     2881 ctttgattaa tttttttcaa attgtttatt aagactaatg ctgtcttttt gcagatacca
     2941 atgttcgaac tttttaacat tatcatatga tcctgcaagt tttaacttct taccattaga
     3001 tgttatcaaa gttataatta accttgcctt tttcccatta ggatctttta ttttatgaat
     3061 tttctgcagg taatctctcc taatggcagc tatttttacc tcatcagtgc tgataaacca
     3121 ttgatcatcc acatacatac tgcctgttat gttgtgtttt tccatttctt ccgagaacga
     3181 aaagtttaag gctcgttctt acttccaaag ttgaatgagt ccatggattg gacttagtgt
     3241 gagaattgag aacaacaata agaatccata tcctcccgca aacatcccta aggctatact
     3301 gtctctccat ccataagtag gattagaaga gaaaagagtc accgtttgta ataatatgaa
     3361 aaggaataat ccagcacaaa caccaaagat aacaactcca aacattgaat ttttatacaa
     3421 tgaccattta tttataggct tttctttcat tttacttccc cctactgttc tgttcacacg
     3481 gaatgaattc aaagttcata agccctcctc tttgaacata tgaatacgac ttgctaacta
     3541 tactttaaaa aagcttgttt taagcggtta taggcgagtt gtaatctcta ttcatctcat
     3601 tagcaaataa cttgccgaaa agtctactga aattaaagca gatagtggaa gaattattta
     3661 gccaggatag acactatttg tgaaacacat gtctttactt ttgaaattct atgatgaaaa
     3721 gccttggaat acagtagctc ttaattggta atactttttc cattataaat aacctctttt
     3781 cattttatat ttatcccctc taaaaataat atctaatcta atattttaaa cttatatttc
     3841 cctcgctgtg ctaatagagc attactaaat aaatagaaac tagaaaaaag cattacgcct
     3901 gccagaataa accaattatt tgtat
//
LOCUS       DACEUZ010000164         3817 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.164, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000164 DACEUZ010000000
VERSION     DACEUZ010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3817
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.164"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            352..1041
                     /locus_tag="IXG44_002441"
     CDS             352..1041
                     /locus_tag="IXG44_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1659135.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1028..2236
                     /locus_tag="IXG44_002442"
     CDS             1028..2236
                     /locus_tag="IXG44_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ1659136.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2530..3801
                     /gene="serS"
                     /locus_tag="IXG44_002443"
     CDS             2530..3801
                     /gene="serS"
                     /locus_tag="IXG44_002443"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HAQ1659137.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
BASE COUNT         1258 a          658 c          771 g         1130 t
ORIGIN      
        1 tttccaacat gtcaacaact tattttttat tatttaataa gttattggtg ttcttttgaa
       61 caaataataa tatatcaaca tcacatgtga aagtcaataa gaaaatgctt atttatcaac
      121 atttctagct tacatgatca ttggttagtt ctggtccttt ttcgttcgtc tttataactc
      181 cgagagacgt aaaatccttt tgttttcagt ttaatgttca tgttcttgtc ttattcacac
      241 caactagatg tctcaccttc taaaattttc cttaacaaag tgctgctttt atctatttct
      301 gctttttttt gctagaataa tagtaacaat atgactctgg aggatttatt tatgaaaatt
      361 ttgattgctg acgatgataa agaaatcgtt gaattattaa gtatctatgt tcacaatgag
      421 gggtatgaag cagtaaaggc ttatgatggg aaagaagcat tatctaaaat cagaacaatc
      481 cctgatattg aattacttat tttagatatc atgatgcctg agatggatgg gatgcaagta
      541 gtgaaagagc tacgaaaaga atcccaaata ccaatcatta tgctgacagc aaaaactact
      601 gatatggata agatcaaagg actggtcgct ggtgcagatg attatgtgac caagccattt
      661 aatccgttag aagtaatggc tagagtaaag tctttattac ggcgtacaaa aatgcagatt
      721 gccccagatc agcctgatgt attagaagta aatgcattaa tcatcaataa agactctcat
      781 gaagttaaaa caatcgatgg gaaagaaatc cagttaacag ctttggaatt cggtatcctc
      841 tacttgcttg ctagtcatcc taatcgagta tttagcgcgg atgaaatttt tgagcgtgtt
      901 tggaaacaag aaagtattgt ctctgctaaa actgtcatgg ttcacgttag tcatttaaga
      961 gataagattg aagaagcaac tggtggtgaa aaagtcattc aaaccgtttg gggagtgggc
     1021 tataaaattg acgcaagata gtaaacgagc aactgaaaaa cgccgtcgga tcaatttgac
     1081 ttcaaaagaa atcagcgaat tgttagcgga aggaatcatc acgattatcc ttttgcttct
     1141 attaaatgtt tctattctgg ttgttatcag ttcggtgatc aacagttcac cttctttgac
     1201 gaatgcgatt tgggattcaa agaatatttt tgcagaacga ttgaacactg atttgttctg
     1261 gaacggccgg aattttatta ttcccttttt ctttctatta gatattggtg ttttgtattg
     1321 gcgcttgatt cgaagatatc gccaaatgca attgcggcat atcatcagcg aacttcacta
     1381 catcgcaaac ggaaattatg atcatcgtat cccttttgaa ctgagtggtg acttaagccg
     1441 agtggtaaca agtatcaatg gattggtgga tagtaccgta gcagcgattg aagacgaacg
     1501 taaaatcgaa aaatcaaaag atgaattgat cacgaatgtc agtcatgata tccgtacccc
     1561 actcacgtcc atcattggat acctaggact gatagaagat ggccagtatc actcagaaga
     1621 agatctttta aagtatacgc atactgctta tatcaaagca aagcagatga aatctttagt
     1681 ggacgactta tttgaataca ctaaggtacg ccagcctgct gtcccggtga atttttctgc
     1741 ctttgacatg atccagttga tcgaacagtt agcagctgat tttgagttgg aagcttctaa
     1801 aaaaaatatc caaatacttg ttcaatcaaa agtagactcc cttatcatgg atggcgatac
     1861 cgaaaaactt gtacgtgtct tcaataatct tttgacgaat gcgttgaaat acggaaaagg
     1921 agcaacgaag atcgtaatcg aagtagaacg tatcggttca gaagttgttg caacagtcaa
     1981 aaataacgga gcaatgatcc cacaacaggc aatcgataat ctattcgacc gtttttatcg
     2041 tgtagaagaa tctcgttcac aagcaactgg aggaactgga cttggtttag caatcgctca
     2101 aagcattgtg gctttgcacg gcggatacat ttatgcaaaa tcagataagc aatggacttc
     2161 tttcatcatg caccttccaa tcaagaaaaa tgaaaaatta cctattgata cacaggaagt
     2221 tattgacgaa agctgaaata ttcgctattc ttattacaat tattagaata aatgaaaaac
     2281 acagaaccca gtacacttta ttctttttca gagagctgat ggcaggtgta aatcagcaaa
     2341 ggataatttg gaagttccag ttcgtttacg tgccctttcg aaataagaaa ggtcggttca
     2401 acagccgtta tgtgttcaac aagtgcagct gacgctgaac ttgggtggta ccgcgaattt
     2461 cagactgatt cgtcccaaga ataatgggat ggatcagtct ttttttattt tcaaaaagga
     2521 ggaaatcata tgttagatgt aaaaatgatg cgacaaaata ttgaagaagt acaagaaaaa
     2581 ttgatgactc ggggcgtaaa aaaagaaaca ttagctgaat tcatggaact agatgaaaat
     2641 cgtcgtaagc tactagtcaa aagtgaagaa ttaaaaaaat atcgaaatga tgtttctgct
     2701 gagatcgccc aattgaagcg tgcaaaagaa gatgcttctg ctaaaatcgc tgaaatgaaa
     2761 gaagtcggtg gcaacatcaa agcattggat actgaaattg ctgaagtgga tgaaaaattg
     2821 aaacaaattg caactacttt gccaaacctt ccccatgatt ctgtgccagt tggaacagat
     2881 gaagaggaca atgtagaaat ccgtcgttgg agcgaaccac gtacatttgc ttttgaacca
     2941 aaaccacact gggaaattgc agaaaatctt gatattttag attttgaacg tggcgcaaaa
     3001 gtagccggta gccgttttgt ctattacaaa ggattaggtg ctcgattaga acgtgctgtc
     3061 tacaacttca tgcttgatca gcatatttac gaacacggct atactgaaat gatcactcct
     3121 tatatcgtca acagtaaagc gatgtttggt acgggacaat ttccaaaatt caaagaagat
     3181 gttttccaat tggagaatac tgatctaaca ttaatcccaa cagctgaagt accattaact
     3241 aactattata atgatgaaat tttagatggt gcagatttac ctatttattt cactgcacta
     3301 agccctgctt ttcgttctga agccggaagt gctggtcggg atacacgagg actgattcgt
     3361 cttcaccaat tcaataaagt agaaatggtc aaattttcgg atgtagagca ttcttacgaa
     3421 gaattggaaa aaatgacagc tgatgcagaa gatatcttga aaaaattgca tttgccttat
     3481 cgtgtgatgg ccttatcaac tggagacatg ggcttttctg cagctaaaac ttatgactta
     3541 gaagtatgga tacctgcaca agatacttat cgtgaaatca gttcttgttc taactgtgaa
     3601 gatttccaag cacgccgcgc aatgatccgt tatcgcgata atgatggaaa aatccactat
     3661 actcatactc tgaatggttc aggacttgca gttggccgta cagtagctgc cattttggaa
     3721 aactaccaaa atgaagatgg ttctgtcaca gttccagaag cgctcgttcc ttatatgggg
     3781 aatctgaaag ttattaaata aagaggctgt gaaaaag
//
LOCUS       DACEUZ010000165         3721 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.165, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000165 DACEUZ010000000
VERSION     DACEUZ010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3721
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.165"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            369..998
                     /locus_tag="IXG44_002444"
     CDS             369..998
                     /locus_tag="IXG44_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1659138.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(1122..2231)
                     /gene="ugpC"
                     /locus_tag="IXG44_002445"
     CDS             complement(1122..2231)
                     /gene="ugpC"
                     /locus_tag="IXG44_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HAQ1659139.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            2440..2862
                     /gene="mgsA"
                     /locus_tag="IXG44_002446"
     CDS             2440..2862
                     /gene="mgsA"
                     /locus_tag="IXG44_002446"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HAQ1659140.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            3185..3721
                     /locus_tag="IXG44_002447"
     CDS             3185..3721
                     /locus_tag="IXG44_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAQ1659141.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
BASE COUNT         1088 a          680 c          737 g         1216 t
ORIGIN      
        1 tttttcgttt gtttgggctt attaccgcag atgcaagctt ttgaacaccg tttaagaaag
       61 aggttgtggg cctgccgttt aactctaagt cacaaggaac aatttcaaaa aacagcttct
      121 catgtttttg agttagttgg tcttattact tgatgcaagc ttttgaccac tattatgccg
      181 aacaaggaga tgtgacacaa attttgtcac atctcctatt tttgtttctt gatcaaaaaa
      241 ttcttatact tgatactcac actcattttg tatggattta gaaaagaata tgtgtcttga
      301 cagttattag taagtgagtt atactaagtc gacatttatg tgtcaagaca tattaggaga
      361 gagaaaaaat gagaaaaaat tcagttcgaa aactaacgat ttctgcttta ttgattggga
      421 tgggggtcat tatcccaatg gtcatgccta aaatcatgat tccaccagct tcctttactt
      481 tggcgtctca tgtgccatta tttattgcga tgttttttac accaggagtt gcagtggctg
      541 ttgcgctagg tacgacattt ggtttctttt taacaacgcc ggttatcatc gcattgcgag
      601 cattgtcaca tcttgtgttt gcgttgattg gggcttggta cttgcaaaag catccgagta
      661 tcgttttaaa aaatggacag tttacttttg ctaattggcg tttccaaggg ttcaattttg
      721 tgatcggtgt gattcattct ttagcagaaa tgctagtagt cagcatcttt tactttattg
      781 gtaatatgcc tgaaacatat tattctcaag gatattttta tacagtattc atcttgatgg
      841 ggattggtgg gcttatccac agtttagttg attataatat tgcttatttt atagcaggaa
      901 cactcagcaa atattttgat atccccgtat ttacggcagc aaaaaaagaa aatcggaaag
      961 agtcgattca agggtacgcg aagaaaacga catattagac atgatctctc aaaaaaaaaa
     1021 ctctcatcta gtaatggata ataaatcctc taagatgaga gttttttgtc ctaaaaagag
     1081 ctgcaccaac atcttctggc acagctctaa cagggggcaa tttatggaag tttgatgact
     1141 tgttctgttt ctttatcaaa gaagtggcct ttgctgatgt tgaaggcaag gtcgactatt
     1201 tcgcctggtt tatggaaatc tcttgcatct acttttgaga tgaactcagt agaaccagtt
     1261 tttgtgtaaa gcatcgtttc agctccaagt aattcggaaa cgacaacttc tgatttcact
     1321 acggcttcag gtgaagcttc aagggctact tgttcactgt ggatgtcttc tggacgaata
     1381 ccgaagataa cttcttttcc ttcataacct ttttcaacta atactttgtt ttttccttca
     1441 ggaatacgaa gcttaagccc atgtccatct gtgataacac catttttcaa tgtcacatta
     1501 aagaagttca tggcaggaga tccaataaag ccggcaacga acatattgac gggtgtatca
     1561 taaacttctt tcggtgtacc gatttgttgg ataaatccgt ctttcatgat aacgattcgg
     1621 tcagccatag tcattgcttc tgtctgatcg tgagtaacat agatggtcgt tgtttcaaga
     1681 cgctggtgaa gtttggcaat ctcagcacgc atggctacac gtaatttagc atctaaattt
     1741 gataatggct catccattaa gaagactttt gcatcccgaa cgattgcccg tcccaatgcc
     1801 acacgttggc gttgtccacc agataaagca gctggttttc gtttcaagta ttctgtcaga
     1861 cctaagatct ctgctgcatt gtccacacgt tttttgatgt ctgctttatc atatttacgt
     1921 aatttcaagc caaaagccat attatcaaaa acagtcatat gaggatacaa tgcgtagttt
     1981 tggaaaacca tggcaatatc acggtcttta ggtgccacgt cattcatgac ttttccgccg
     2041 atatttagtt ctccttcaga gatatcttcc agtcccgcga tcattcgcaa agtagttgac
     2101 tttccacaac cggaaggacc aacaaaaacg ataaattcac ggtctttgat tgatagatta
     2161 aaatcagtta cagaataatt ttctgcattg tcatattttt tatatacgtt tttcagagcc
     2221 atttctacca taatacgttc cacactttct tttttagttt tattaactat ttaaagtata
     2281 aatgaaagcg ttaactaact tcaatgtcat gttgaacaag aattttttct tttttttgtc
     2341 aatgtgctag ttttaagctt taataaatat ccataagaaa taaaaactga tacactggag
     2401 taaataagta tgttaacaat atcaaaagga gtgtctctta tgaaaatcgc tttgatcgct
     2461 catgaccgaa aaaaacctat gatggtgaaa ttagcaacag cgtataaaac aatattggaa
     2521 aaacatgaac tttttgctac cgggacgact gggcagagga tcacggaagc aaccggcttg
     2581 ccgatccatc ggtttaaatc cggaccgtta ggcggggatc agcaaattgg agccatgatt
     2641 tcggaggatc agttagattt ggtgattttt cttcgagacc cgctggctgc acagccccat
     2701 gaaccagatg taacagcact tatccgttta tctgatgtct atgagattcc gttagctacg
     2761 aatatcggaa cagctgaggt gctactaaga ggactggaag ccggcttcct agattttcga
     2821 agaatcgtcc atgaaataga caagcatcca ctttcatttt gatggataag cgtatggaaa
     2881 aataacgttt aatgttcgcc tatatgtagc tataaaagct aaacaaactg ataatatgcg
     2941 atataatacg aacgtattgg ataatataaa caataatatt agtttttcgt atttgcagta
     3001 aatgtgaaaa tatgctatag tttctgtggg aaaagatccc aaaaataata cagagtagaa
     3061 aataaagcgt taagtgctaa gaggatggga tgttgcctct tggacgaagg acgtaatgtt
     3121 cgcggtttta ttttcgcatt cgctgcatga tacgatgtag cagctcttaa aggagatgct
     3181 aaaaatgaag aaacaccggt tagacgcacg tatgattgct attatgggct tattgattgc
     3241 attgatggtc actttgtccc gactggtagc aatcgagacc ccatttatta aaatcagtgt
     3301 cacatttatt ccacaagtga tcatgggcat tctttttggt cctttttggt cagggatagg
     3361 cgcagtttta gctgatttag taggaatggc cctcttctcg aaatctgcat tctttattgg
     3421 atttacattg aatgcattta ttgaaggagc aatctacgga ttctttttct atcgaaaaga
     3481 gatcacttgg aaaaatgcca ttctggctac gcttagtgtc acgctgatca tcaacttatt
     3541 tttgacacca ttatggcttg cgttgatgta ccatgtgcca ttgttcagct gggtcgtttg
     3601 ggcgccccgc ctgttaaaaa cagtaatatg gttaccgatt caatcaattg ccatttatta
     3661 cgtaggacgt agtattcctt acaaaaaaat tttaagaagt ttagcgatcc acgctaaata
     3721 a
//
LOCUS       DACEUZ010000166         3719 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.166, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000166 DACEUZ010000000
VERSION     DACEUZ010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3719
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.166"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            122..1321
                     /locus_tag="IXG44_002448"
     CDS             122..1321
                     /locus_tag="IXG44_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAQ1659142.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVLLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYLAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(1348..2616)
                     /locus_tag="IXG44_002449"
     CDS             complement(1348..2616)
                     /locus_tag="IXG44_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HAQ1659143.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLCLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVCRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
     gene            complement(2779..3425)
                     /locus_tag="IXG44_002450"
                     /pseudo
     CDS             complement(2779..3425)
                     /locus_tag="IXG44_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FusB/FusC family EF-G-binding protein"
BASE COUNT         1095 a          779 c          653 g         1192 t
ORIGIN      
        1 taattgaaca ataagaaaaa catctttcca tcggaaagat gtttttttgc tttttctagt
       61 ttaaataaca gcttttttca tctattttcg ttatactgta taagaaaaag gagttgaatt
      121 tatgaatcta accagagatc tgatcttttt agttctctat cttattgggg tattctctat
      181 cgtttattgg cgaaaacaga agaaggatcc ttccttgttc ttttacggca gtacgtggac
      241 atttacggta atcttaactt attttgtcgt gttggaaatt ttttattcga ccttttattt
      301 tttgattcca ttgttgttct ttagtatctt tgctttttct tatttcacag aaaaacgaag
      361 gctactgaat ggtcttttat tcaatgtttt actcatcagt ttcggcatct acttgtttgt
      421 cttgctgtat gaaacgcaaa atatttttct tggcggactg atcgccttga tcacgattcc
      481 attgctactt gtccttttat ttggtattta tggattgatt gtttttcttt tttggaacgg
      541 ggtcacagtt ttacgtagag aatcgcattc attagctaat cttttaacgt tgatactagc
      601 catttttctt acactgtttc ttgtttttga ctttttcttg ttaaaatatt tgccgcaatg
      661 gataaatgct ttgtttttct gcgttcccct gatcttgatc tatctattta tcgtattcta
      721 caactttttg actgtctcat tcttgtatca gttcaaccgg cctcgataca atcaagactt
      781 catcgttgtg ttaggcgctg gattgatcaa tggagaaaca gtttctcctc ttctagccaa
      841 gcgaatcaat aaggcaattg ctttttatcg ggctcaaagc cgtgctacat taaatccgcc
      901 catcttattg atgtctggcg ggcaaggagc agacgaaaaa gtaccagagg ccattgcgat
      961 gaaacaatat gccatggaac aaggaatccc tgaaagagac attcttgtcg aaaccaattc
     1021 gacaaccact ttggaaaaca tgctctattc aaaggaaatc atggatcagc aaatgaaagg
     1081 gccataccgc gcgatcttta gttccaacaa ttaccacatt tttagagccg gtctttatgc
     1141 gcggcaagcc aaattgaaag caaatggtat tggtgcaaaa acagctttct actacttgcc
     1201 taatgcgttt ttaagagaat acctcgccat cttgatgatg aacaaaaaaa gacatatgat
     1261 catttgcgga ttgcttttcg caggctccgc attcttatcg ctgattactt tacttttcta
     1321 aaaaaacaag gtgtgacaaa acatatgtca caccttgttt ttttctttca ttctcttaac
     1381 tattggcaaa aagagtatcc cttttttgac cgctacttgc tgagcagaat aaataccgct
     1441 gttgcctgaa cacataaagc tgatcaagca gatcataaag aagtatactg ccgcttgtga
     1501 accgaacaat tcgatcccca tcacaaagca ggcaatcggt gtatttgtcg caccagaaaa
     1561 tactccgata aagcccaatc ctgcaagaaa aggaacagaa acatggagca aaggcgctag
     1621 tgctgcacca agcgttgcac caatttcaaa caatggtgtc acttctcccc cttgatatcc
     1681 cgcgcctaaa gacaaaactg tgaagaataa cttcccaata aaatcataga catgtgcctg
     1741 tccattaaat gattcttgaa gtaacggaag actcaatccg agataacgtt gtgtttgcaa
     1801 aatcaatgct gctgccacaa ctacagctcc acctatcgta tttcgacaaa caggatgagg
     1861 aatccattgt gcatatcgcg cttttaaaaa gacgatcgaa cggctgaata cccaaccaat
     1921 caaaccaaaa gcaatcccag caacaaaaac tttgaagaac agcaacatac tccattcagg
     1981 aatcacaccc atttggtaat gtagatgaga aacaccatag ctttctgtca tgaaatttgc
     2041 aaacagtgca gcaaagaagc tagggaagat cgcttctgtt cggactttcc cgattgccaa
     2101 cacttctaat ccaaataatg ttccagctaa aggtgtgcca aatacagaac taaaacccgc
     2161 actgattcca ctgataatca agatttcccg ttctgattta tccaatcgga acaaacgtgc
     2221 cgtattatct gctaacgctc cccccatttg gactgctgtt ccttcacggc caacagatcc
     2281 cccaaataaa tgggtagtta tggttccaaa cagtgtaagt ggaataagcc gcaacgggat
     2341 tttttcttct ccaccatttc cttgatcgat cactaagttg tttccacgac tagaaagtcc
     2401 gccgtgatac gcatagagat aagcaaaaag cgcaccacta actggtagta gacaaaggag
     2461 ccaaggatgt gatagtcgga tatctgtcac aactgtcagg ctatttaaga aaaaagcaga
     2521 caagctcccc atgaatatac cgagaatcgc tgtgatcacc agccacttca gcatatacac
     2581 taaaatgcgt actggactcg gtaattgttt ttccataaaa aattcctcct caataaaaaa
     2641 acgcctacta aagaaagggg ctgtccccag actttagtag gcgtcattag ctatctttcg
     2701 ttagcattta aaggcgaaca ccatcaccta ttcacttcca ttaaaagata gcatattttt
     2761 ctgtaaaaaa caataaaatt atcgttcttt ctttacacgt tcagcaaatt catagaaagg
     2821 agccaatttc gttaactgtt gattacattg gtcactatca tgacaaatat aattcccctt
     2881 tttcgtatac gtgccatcag aacctgcttt cgttgtagaa agaaaaagcg aaacattcga
     2941 tgttttctga cagattgcac agattccctt atttatcgtt ggtgataaag taccgctaat
     3001 cccttttaat ttttcatctt cgtaatagac catgaatttt ttctgcgtac ctggatcatt
     3061 ccagccgatg tacgtatgtt cttgcaagtc gatttcattc caagctggtt gtttgagttt
     3121 tttcactttt cggaataatt tctcgatttg tggtttgcta ggctgcataa acggctgaac
     3181 gatttgcttc agctcctcca atgctctttc tgcttgcgct tttgtcagtt ttacgtctaa
     3241 aactgtcgtt aaaaatgtat cgattctggc agttcgtgat ctaatatgtc tcgaatcttt
     3301 tcagctgcta gtgcctgaac tgtttgtacc gtttttggat cattgacaga tgagtacgca
     3361 tttaataact gttctacttc tcttattata aaaaaatatt ggtatggttt tattgtttgt
     3421 tccatgattt taaagtcctt tcttctaacc aaagtgcata ctttgtagaa aaaagtgatc
     3481 ttctgcaaag tatgtgttac ctgaagatcc cggactcttt taaataagat acgttttgca
     3541 tcttatcatc acccccttta atgcttatta ttctatgtac atgattcatt cgataaataa
     3601 aagtaaaccg tatcttgaac agatagtcaa ctgcaaacag aaaaaaggac tgtgacaaaa
     3661 gtcgtgtcaa agtcccttgt ttcgaatgaa tggtgttcaa aagctggcat ctgcggtaa
//
LOCUS       DACEUZ010000167         3630 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.167, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000167 DACEUZ010000000
VERSION     DACEUZ010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3630
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.167"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(152..973)
                     /locus_tag="IXG44_002451"
     CDS             complement(152..973)
                     /locus_tag="IXG44_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAQ1659144.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(1070..1348)
                     /locus_tag="IXG44_002452"
     CDS             complement(1070..1348)
                     /locus_tag="IXG44_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1659145.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            1616..3625
                     /gene="metG"
                     /locus_tag="IXG44_002453"
     CDS             1616..3625
                     /gene="metG"
                     /locus_tag="IXG44_002453"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HAQ1659146.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
BASE COUNT         1206 a          667 c          713 g         1044 t
ORIGIN      
        1 aaagttcgta cgagcttttt ctgctattgt taatttttta ttcagaaagt ttacgaatac
       61 atcaaaattc tgaaacaaaa aacgtaactc acaaagagtt gtttttgcgt tttttcttga
      121 atgattaggt ctgaatcttt tgctcacaac cttattcatg gtgccccggc ttgagctgat
      181 agatttttga aggacggccg attcctacag gacgttcacc gatctcatcg aaatattgga
      241 gcatagcttt cttaaaattg gaatgatcga tcgttttata atcgatccct aagaatttag
      301 caaatacttt tcgagcttcc gtaatcgtga aatctttacc taaaacttgt agaacttgcg
      361 gctcgtgttc cattttattg actacacggt tgaatgcttt caagatgatt tgactatggt
      421 caaaagccaa agtatctttg ccagaagact ctccagtgac taaatcaagc gagatagcca
      481 cttctccgct agataaatgg atcttgttac tatgacgttc tagtgtgaac cagcgtactt
      541 ccttggcatc atctccggcg attagcggtt cttctccaat aaaggctaag tagctgactg
      601 tgacgaccca gcctcgcgga tctcgattag gcgtactgaa tgtatgcagt tgctcaatat
      661 tttcttttga aataaccaca ccagtttctt cctttgtttc tcttaagacg ctttctccag
      721 ttgactcatc cttttggaca aagccaccag gcaatgccca tgaatttcga taaggatgcc
      781 cttttctttg gatcaacaaa actttcagct gatcttcttc tttgttatag cacatcagta
      841 cgatatcgac agtcaaagag ggcttgtcat attctggacg ttcctgtttt tgataccagt
      901 taagaaattc ttcttcactt gctttatgtt cataatataa tttttcttct tctttagaag
      961 caaaacggtt catctgacca cctccattga cagtaaaagt actttttacc ttattgtagc
     1021 agactctgaa aaaaaaccaa ggatttactt gaaaaatcta ggttatcatt taaatatgaa
     1081 aaataaatgg aagtatgaga ttgaataaaa agaaaagcag ataaaacggc aatactttat
     1141 accgctcttc acgaatggca aagaagccac aaataacgat atatacgtca atgataaaac
     1201 gcaaaatcca agctgctgtc ataaaactgt tcaaatgccc atggaggtta aaataaccat
     1261 acatacgaat ataaaaatag ctgaatatta aatgtgctaa tgaaagaaaa gcaatactaa
     1321 gtgtaaaaaa aagatgtttc ttctgcatga ttgttccagt cccttactaa gatagatagt
     1381 ctttattata ccacgtgtga ggggagaaat aaaaagccgt tttttagtag atatgaaaca
     1441 aaacaaaaac ccgcatttat tcaacctatt catcctttta aaggatagat tgaacgtaga
     1501 tagaagtcgt ttatccagtc tttttcattt tgaaaaaaag ctaacagcat gcttttttta
     1561 tggtatacta gcaaggaaac gaattcgcat catgcgataa aggaggaaga gccccgtggc
     1621 agaaaaggaa actttttata tcactacgcc aatctattat ccaagcggca aacttcatat
     1681 cggcaactcg tatactacga ttgcatgtga cgcaattgct cgttataaac ggttgatggg
     1741 ctttgatgtg ttttatctca ctggagtgga tgaacatggt caaaaaatcg aaacaaaagc
     1801 agaagaacta ggtgtaaaac cgcaagaata tgtagataaa atggcggctg atgtcaagaa
     1861 attatggaaa acgcttgata tcagttatga taaattcatt cgcacgacag atgattacca
     1921 taaagcagct gttcaaaaaa tatttgatcg tttattagaa cagggagata tttacttagg
     1981 tgaatatgaa ggctggtact ctgtttctga cgaagaattc tttacagaaa cacaacttgc
     2041 agaagtgtac aaagatgacg aaggtaacgt gatcggtggt aaagcgccaa gtggacacga
     2101 agtagaattg gtaaaagaag aatcatactt cttccgcatg agcaaatacg cagaccgtct
     2161 attggaatac tatgaagaac atcccgaatt catccagcca gaatctcgta aaaacgaaat
     2221 gatcaacaac tttatcaaac caggattaga ggatcttgca gtttcacgaa caacttttac
     2281 ttggggagta ccagtcaaga gtgatccgaa acatgtggtg tatgtatgga tcgatgcatt
     2341 atccaattat attactgcac taggatatgg ctccgaagat gaaagtctat tcgaaaaata
     2401 ctggcctgca gatgttcaga tggtcggtaa agaaatcgtt cgctttcata cgatctattg
     2461 gccaatcatg ctgatggccc ttgatttgcc tctacctaaa aaagtatttg ggcatggctg
     2521 gctattgatg aaagacggaa aaatgtctaa atcaaaagga aatgtcgttt atcctgaaat
     2581 gcttgtggaa cgttatggtt tagatgcact ccgttattat ttgcttcgtg ctgttccgtt
     2641 tgggtcagat ggtgtcttta cgccagaaga ttttgtttcc cgtgtcaatt atgatctggc
     2701 aaatgatcta gggaacttat tgaatcgaac gattgctatg atcaacaaat attgtgatgg
     2761 actcgttcct gactatgcct cacttgtcac tccatttgat agtgaactgt ctacgacggc
     2821 tgctaacgtt gtcggacgtt accatgatgc aatggaaaaa atggaattca atacagcatt
     2881 ggctgaaatc tgggtgctga tttctcgtgc aaacaaatac attgatgaaa cagaaccatg
     2941 ggtattagca aaagacgaag aaaagaaagc tgaattgaat agtgtcatga tccatttagc
     3001 tgaatctttg cgtatcgcag cgatattact tcagccaatc atgacagaaa cgccagagaa
     3061 gatcttcaat caactaggat tggactctga aacaatgaat cttgaaggat tacactttgg
     3121 cgaatttcct tcaggaacaa aagtagttgc aaaaggtaca ccaatcttcc cacgtttaga
     3181 tatggaagaa gaagttgctt ttattcaaga aaaaatgtct gaaggtactc aaacaaacga
     3241 agatacagtc aaatgggatc cagaagaaac agaacttgtt tcaacgaaag aaaaacaaat
     3301 caaatttgat gtatttgaaa aagtagaact aaaagtagca gaagtcatca actgtcaaaa
     3361 agtagaaggg gcagataagt tgctgcaatt ccgactagat gctggagaca gccaagaccg
     3421 tcaaatcttg tcaggaatcg ctgaatttta tcctgatcca agcgaattga tcgggaaaaa
     3481 agtcgtcatt gtagcaaatc tgaaaccacg aaagatgcgt ggacaaatca gtcaaggaat
     3541 gatcctttct gcagaagcac cagatggctc tttacaagta atcgaagcac caaaatccat
     3601 gccaaatggc tcagaaatcg catagaaatt
//
LOCUS       DACEUZ010000168         3601 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.168, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000168 DACEUZ010000000
VERSION     DACEUZ010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3601
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.168"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(<1..158)
                     /locus_tag="IXG44_002454"
     CDS             complement(<1..158)
                     /locus_tag="IXG44_002454"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1659147.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDI"
     gene            886..1854
                     /locus_tag="IXG44_002455"
     CDS             886..1854
                     /locus_tag="IXG44_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1659148.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            1872..2777
                     /locus_tag="IXG44_002456"
     CDS             1872..2777
                     /locus_tag="IXG44_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1659149.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            2774..3580
                     /locus_tag="IXG44_002457"
     CDS             2774..3580
                     /locus_tag="IXG44_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1659150.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
BASE COUNT         1118 a          742 c          712 g         1029 t
ORIGIN      
        1 aaaatgtctt caaacatttc tctaagtccc tcatctaaga accattggta agagtctgtt
       61 tgaatttcga tcaaatttgg taattccaac acttcactga ttcttgcgaa acttctacgt
      121 tcgcgatgtt ttccgtattt tactacgtgt ccagccaagc tcttcacccc tctaaaatgt
      181 cattcaaaaa gactacccga tcaattgtta caataatatt tcaaatatta tttttaggta
      241 acaacagaca ttttttgcgg cttttaggca aaaaaaaacc aaaaatagaa cattctcttg
      301 aaaccttact tcgtgagctt ctacttttgt ttccttatat taaagccgaa acggacaata
      361 tttcgtttct gccttttctt catcagatag tttttgcaac ttcatatact acacttattt
      421 tctataaaag tcaacacttc ctggcgtttc ttctgctttt tcgcaacatt ttcttaatgt
      481 tttcaatttt atgttttcaa ttatctgaca attcttaatt gacagacctt tttatctcgg
      541 ttatacttta gaaaattcat tttgtggagg aacttctatg cgaaccaaca tcaaccaaat
      601 cgcaccaaca caactgaata acttttactt tagctacttt agataggttt gtattcatca
      661 tgatggatac aaacgttaaa cgacagtttg tatctatgat ggctaaagaa ctttgtattg
      721 cagcaaaaag ccacgtagac aaaatgctaa gtggctgagc agtgaaaata caaagtaaaa
      781 gtatcaagca ttctactttt tcaatttatt ttccgacttt tcaatccatt tggacatttt
      841 gtctgaatgg attttttgtt tcccaaaatt aaggaggaaa aagaaatgaa aaaaaatata
      901 atcatcggat taggcgctgc cctgctacta gctttaggag gttgcgcaca aaaagacaat
      961 tccgcagagg catcagatgg acaaaaaaca attggcgtat tgcaacttgt acaacataac
     1021 tcgttagatg ctgcctataa aggattcaaa gaaggattag cagaagaagg gtataaagaa
     1081 ggcgataatc taacaatcga ttatcaaaat gcccaaaaca atcaagacaa cctgaaaagt
     1141 atgagtgaga aactcgtcaa agaagatcct gatttgctac ttggaatcgc cactccagca
     1201 gcacaatctt tattaaatga aacaaccact atcccgatga ctgtcacagc ggtcacagac
     1261 ttggaacaag cccatttagt cacatccaat gaaaaacctg gcggcaatgt gactggcacg
     1321 acagatatgg tgccaatcga aaaacaaatc ggcttactgt taaatatcgt gccagacgcc
     1381 aaaacaatcg gcatcatgta taacaacggt gaagcaaact ctaagatcca agctgatttg
     1441 gcagaaaaag aattgaaaaa agcgggcgtc aaagtcaaag tactgacagc aaattccaca
     1501 aatgatgtcc aacaagtgac gaaaagtcta gctaaagatg tagatggaat ctatattcct
     1561 actgataaca cgttcgcaag tgcggcaagt gttgtcggtg aagtcgcaaa agaaacaaaa
     1621 acacctgtcg ttgctggcgc agttgatcaa gtcaaagacg gcggtctagc aacagtagga
     1681 atcgactacg aaagcttggg caaacaaaca gggatcatgg cagcaaaaat cttagacggc
     1741 aagaatccaa gtgaacttcc tgtcgaaaca gcgaatgatt tggaactgta tgtcaataaa
     1801 gacatggcca aagcattagg catcaatcca gacagcatca aagaagctga ataaaacaca
     1861 ggaggattct catgacaaat ttgctctctc ttttcttatc atctgcatcg caaggggtct
     1921 tatgggccct acttgcgatt ggtgtatacg tcactttccg tatcttggac attgccgact
     1981 tgactgctga aggaagcttt cctttaggtg cagggatcgc ggcagtaagt attacaaatg
     2041 gctatcatcc attgacagct tgtcttctag gctttttagg gggtgcggca gcagggcttg
     2101 tttccggact gcttcacaca aaattgaaaa ttcccgccct tcttgcaggg atcatcacca
     2161 tgactggttt atattctgtc acatcccgtg tgatgggtgc gccgaacatc tctttattgg
     2221 gtcagccaac gatctttcat tgggtcgagt cattcggcat aacaaaagac aacgctgtac
     2281 tgatcgtcgg tctgcttttt gctttgatcg ttgtatgttt acttgttttg ttcttacgta
     2341 cagaaacagg acttgctatt cgtgcaactg gcgataatct ggcaatgagc gaagcaaacg
     2401 ggatcaacac agataatatg aagatcatcg gttatatgat ttctaatgga tgcattgctc
     2461 tttctggctc cttgcttgcg caaaacaacg gctttgctga tttaaattct ggtattggaa
     2521 cgatcgtaat tggacttgct tctattatca tcgcagaagt tttgttacgt aaccagccac
     2581 tatttctccg tctcttgact gtcgttttag gtgcggtcat ttatcgtttt attttagcgt
     2641 tagtcttcga actgaatgtt gaaccaagtg attcgaaact tgcttctgct ttcgtgctag
     2701 tgatctgtct ttctttcccg cagatccagc aaaaattcca tttacctcaa aaaaagaaag
     2761 gcggtagtgt caaatgaatg acgtcttaac attggaaaat ctccatcata cctttgaaaa
     2821 aggaacgatc aacgaaaatc atgtcctgaa aggcattaat ctttccttag atcccggcga
     2881 cttcgtgacg atcatcggtg gaaatggggc tggtaaatct actttgctga acagcatcgc
     2941 cggtacattt tctgtcgatc agggtcaaat tttattgaat ggaaaaaata tcacgaaaaa
     3001 atctgttgtc gaacgttcga aatccatcag tcgcgtattc caagatccta aactagggac
     3061 agccgttcgt ttaacggtag aagaaaatct tgctcttgct atgaaacgag gaaaaaaacg
     3121 cggcttcttc cgtggtgtca aaccacaaga ccgaacattt ttcaaagacc agctggttcg
     3181 gctaaatttg gggttggaaa atcgtttatc ctcagaaatc ggtttgctgt ctggaggaca
     3241 acgtcaagct attacgttat tgatggccac attacagcgt ccagaactga tcttattaga
     3301 tgaacacact gcagcgcttg accctgctac ttccaaaacg gtgatggaat tgacagaaaa
     3361 gctgatcact gagcagaatc tcactgcttt tatggtcact catgacatgg aagacgcgat
     3421 ccgctatggg agtcgactga tcatgctgca tcaaggaagg atcgtcgtag atatccagca
     3481 agaggaaaaa cagcagctca cagtgcccca attattagcg ttgttcaaac aaaacagcgg
     3541 cacggcatta aaagacgatc aagtactttt atcttcataa tcagaaagag gatgggacaa
     3601 a
//
LOCUS       DACEUZ010000169         3376 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.169, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000169 DACEUZ010000000
VERSION     DACEUZ010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3376
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.169"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            254..580
                     /locus_tag="IXG44_002458"
     CDS             254..580
                     /locus_tag="IXG44_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1659151.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(697..1041)
                     /locus_tag="IXG44_002459"
     CDS             complement(697..1041)
                     /locus_tag="IXG44_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1659152.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(1117..2364)
                     /locus_tag="IXG44_002460"
     CDS             complement(1117..2364)
                     /locus_tag="IXG44_002460"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HAQ1659153.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVTGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            complement(2361..3167)
                     /gene="proB"
                     /locus_tag="IXG44_002461"
     CDS             complement(2361..3167)
                     /gene="proB"
                     /locus_tag="IXG44_002461"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HAQ1659154.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
BASE COUNT          987 a          666 c          671 g         1052 t
ORIGIN      
        1 ccgtttacca agaggttgtg agactgtcgt ttaactctga atcacaagga acaatttcaa
       61 aaaacagttt ctcatgtttg ttgaatattg tgacttatga ttgaggagtt ggcagaagaa
      121 caccatttat tcggaaaaag gggatgtgac acgatttttg tcacattccc tttttttctc
      181 aaaataatga aactggaaaa ttttcagtag gtaagctaaa attcatttaa gaaaaagaat
      241 ggggggataa tagatgctgg aagaatattt tcaggaagtc gggttgacac tcgttgaatt
      301 acccattgaa ttttcagatg atcggttgat ttatcgtggc tatatcaaag aattgccaat
      361 aatcaatgcg gaaggtgaaa ctagaaaagc gatgcagcaa aaaatagctg gtatgtatca
      421 ggtgtatcgg gaacagctac tggctgaatc agcggaagaa gaaaaagaaa aaacgatgaa
      481 cttgtccatg gatgagctgc ttcgttatta tgatggcgaa acatttgacg gattttcgct
      541 tcttaacgaa gaggaaacag atttaccagg cagctggtag gcgaacgccg tttatatgga
      601 aaaagaggct gtgacaaaat ctttgtcaca gcctctaaaa agtccggcag ttttttgccg
      661 atcttcaagg cactctactt atcgtacgga tattttttag cggaaagtcc gattcaagtg
      721 gtttttattc agctgatatt tataagataa gtgaaaaatc ccagcaatca gtagcgtaat
      781 cgtataagcg attgaatgat cgaaaaaaga atagtaattg attgtcgcta ataagcataa
      841 tgcaaccatg taactaatgt gcaagcgtgt aaccagccct ttttgcccga ttttgtcaaa
      901 atactgcgac catttttccc gttgactccc tatgcttcgt tttttgaatt gaaagaaaag
      961 ggataaaata ccgactccca gaaaagcagc gatattcaga gaaataaggt aaataaaaaa
     1021 aatcattaaa atttcaaaca tattgacacc tttctagttg gatagaacct ttacagaaaa
     1081 aaggcaaaag gcgggaataa tgtttcacac gaaacattat tcgcggattt gtccatcgcc
     1141 ataaatgata tattttgtgg aagttaattc atttaacccc attggaccac gggcatgcaa
     1201 cttttgagta gagataccaa tctctgcacc aaaaccaaat accgatccgt ctgtaaaacg
     1261 agtggaggca ttgacataga cagccgccgc gtccacttca tttaagaaac gttgactagc
     1321 cgcataacta tctgtcacga tcgtttctga atgtttagta ttatgccgat tgatatgtgc
     1381 aattgcttca tccaaagaat cgaccacttt gacagcaagg atataatcta gatattcagt
     1441 atcccaatct gtgtcttctg ctaaagcagc cgtctctaga tattctaacg cacgagcatc
     1501 cgcacgtaaa gagaccccgt gttctaccag ttctttttca atagctggca agaaaaaagg
     1561 tgcgatctca gaatggatca gcaatgtttc tgccgcgtta cagacagatg gacgggaagc
     1621 ttttgcattg acgatgatgt tcactgccat agataactgt gcttctttat cgatatatac
     1681 gtgattattt cctgtacctg tttcaatgat aggtaccgtt gcctgttctt tgactcgttg
     1741 gattaatttt gctccgcctc gaggaatcag cacatctaag tattctgtca atcgcatcaa
     1801 ctgtcctgca gtttcatgag aagtatcatc gacaaactgg atggcataag gagattcgcc
     1861 agcactgacc agtacttgct gcaataattg gactaataat tgattcgaga agaatgcttc
     1921 tttgccaccg cgaaggataa cggcattccc tgatttgaat gctagactag ctgcatctgt
     1981 cgtgacattt ggacgagatt cgtaaatgat gccggtcacg cctagtggca cgcgcttttt
     2041 gccgattctc aatccgtcgg tgttcgtcca catctcatct acttttccaa taggatcgtc
     2101 taacgaagca attttttcga tctctgtagc catggcattg attcgctcag gtgtcagctg
     2161 caagcggtct tgcatcgttt cactgatatt gttttcgact gctttttcta agtctttatg
     2221 atttgcttca atgatcgctt gttgattctt tttgatggtt tgtgccattt gaacaagtag
     2281 ctcatttttg cggtttgtag gcataagtgc tagttgttgc gcgctatctt ttgcctgttg
     2341 tcctaattgc atcaagtcag tcatttcaag tcctccttaa ataaagtacc tacttctttt
     2401 cctgcaagaa tatcaaaaat aattgccgga ttttttccat ttgctaaaat cattgcgctg
     2461 tttgcttcaa atatcctaga agcggctttt aatttactct gcataccgcc tgttccaaat
     2521 gtgctaccag caccacctgc ttgtgccaga atcttttcat cgattttatc gatatagcga
     2581 tacatctgag cctcgggatt tgtagtcgga ttatctgaat aaaacccgtc gatatcagaa
     2641 aggacgatca aaaggtctgc cgcgacaagt tttgccatga tggctgacag ctgatcatta
     2701 tcgccaaatt ttgtcagatg atctaattca tccactgcta cagtatcgtt ttcgttcacg
     2761 atagggatcg tgtccataat caaaagctgt tccaatgtat tcctcacatt tttacgactt
     2821 tcaggataat ctaccacgtc tcttgtcacg aggatctgtg ccgtctgttg attatataca
     2881 ctgaatcttt gattatagat attcatcagt tcggcttgac caacagccgc cgcagcttgc
     2941 tgttcaggaa tcaaagtcgg acgttctgac aaatgtaatt tatgcatacc aacgcctaca
     3001 gctcctgatg agactaagat cacttccttt ccccgatttc taagatcaga aaggacaaaa
     3061 gctaactgat cgattgccgc aagattgata tttccattgg gataaatcaa tgtacttgtt
     3121 cctactttga caataattcg ttttgcattt ttaatttctt tacgcatgtg acacctcaat
     3181 caaatatcca ctcattaatt tcgttctatt ctacttgttt tgacgagtaa agtcatgtaa
     3241 aaaatagttt ttttgtacaa cttgcagtcg tttggaaaaa gaacgaatta atggtttact
     3301 ttaagatagc agaatttctt gaagaatttg taaagaaagt cttttacagc ctcggatatg
     3361 ttagaatagg agtagt
//
LOCUS       DACEUZ010000170         3311 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.170, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000170 DACEUZ010000000
VERSION     DACEUZ010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3311
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.170"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            103..297
                     /locus_tag="IXG44_002462"
     CDS             103..297
                     /locus_tag="IXG44_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1659155.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            566..865
                     /locus_tag="IXG44_002463"
     CDS             566..865
                     /locus_tag="IXG44_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1659156.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(962..1396)
                     /locus_tag="IXG44_002464"
     CDS             complement(962..1396)
                     /locus_tag="IXG44_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1659157.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1628..2338)
                     /locus_tag="IXG44_002465"
     CDS             complement(1628..2338)
                     /locus_tag="IXG44_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAQ1659158.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2328..3188)
                     /locus_tag="IXG44_002466"
     CDS             complement(2328..3188)
                     /locus_tag="IXG44_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HAQ1659159.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3193..>3311)
                     /locus_tag="IXG44_002467"
     CDS             complement(3193..>3311)
                     /locus_tag="IXG44_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase"
                     /protein_id="HAQ1659160.1"
                     /translation="LFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
BASE COUNT         1033 a          644 c          523 g         1111 t
ORIGIN      
        1 aaattgattc tttttattta tcataaagat atagatacat aatcattttc acttacatat
       61 atttgaatat taactaaaaa cagcaagaaa gaagaggatc aaatgtatta tgtagaagta
      121 aaaacaaaag gcgtcaaaaa caaacaatat gtgaaaggta tcagtaacga atatccttta
      181 cttggttcat ggaaagaagc tgctcctttt tccaaaccat gtgccataaa aataaagaac
      241 gagctagaaa aagaactgac ttgtggtaaa gctgttgtag atatcatcga aaagtaaatc
      301 caatgtatag aaaggaagat cacacatgaa aagcgacgta caaattgcta agattataga
      361 attagaagaa gaagacgcag tgttgacaat tgaaaaaaaa taaatgtttg tataccatat
      421 gattatttca atatgtatcc tggaattgga gatctcgtag agctatatca agatgaatca
      481 aatatacttg tcattcatca tgaaatatag agacttataa aattgtttat ttttataatg
      541 tatcagtata gaaaggaaat aaaacatgga ttatactgtt gaatttgaca gtgaactact
      601 taagcaattt ccacaagaag aacatttcat gtataaagaa gcaatttccc acaaagtgac
      661 agagtatttg gagcataatt tttttcgtat aaagccagtg cgtactcgtt cggcaaaagg
      721 aatatacgag atgaaagtca aaattaataa aaaaaattat cgcatagcat ttgctttaaa
      781 aaaaaatcat gtgttcattt tttatattag ttcgaatttg caaaaaattg tatttgataa
      841 agaggtgagt aaaaaaataa aatagctgag cattggagga tagattgagt gatactaagt
      901 ctatcctctt gtttttctaa agaactaagc tagaattttt tcttttgtac gttattatat
      961 gttatttgtc attaccaaaa agcacctttt ttcttctagt atctatgaca taagaaatac
     1021 cacccatttc ctttacttga acaaatccta ctgattcata aaattttttt gttttctcag
     1081 tatcatctgt caataattcg atttggactg gtccataact agtgatcttc tccaaagctt
     1141 cattcaaaag cactttgcct attccttggc gctgatacct cggatgaatg aggatatctt
     1201 gaataaatgc gatcgtcgca aaatcagtga tccaacgaac gattccgata atcttccctt
     1261 ccccattacg ctttatcaga gattcagatt gttcaaatgc tctagctaac cttgcagtgt
     1321 cttttgtgta attcgtccaa ccaacgctct tatataattc agaaatctca tcgatcataa
     1381 gggaatcaat attcattttt ttcttccttt cttatacaat tctccctcta ataatacagt
     1441 tctttgacta tttaatctat cacatataac tctcctgtaa agattctctc gattccttaa
     1501 aattcctctt tccttttaga atgcatactt tttgataaat aaaaaggttg tgataaattt
     1561 tcttcacaac ctctgtttct ctctatcaag tgttatcttt taattttcta cccttaccct
     1621 agcatctcta ttgcccgtag tatgcatact ttccatgttt gcgaaaataa tgtttatcta
     1681 gtaaatattg cgggatttca catatattgg ggttgatcag ctcgttttct ttggccatca
     1741 gacaaatttc atctaagatc aaactgtttt ctactgcttt cataggtgaa tccccccatg
     1801 tgaacggtcc atgtccatag acaagtactc cagggacttc gtttggatct aattttcttt
     1861 ccttaaacgt ctcgacaatc acattccctg tatgaacttc gtaagcttct cttacttctt
     1921 ctttcgttaa ttggcgtgta caaggaacct taccataaaa agcatcagca tgagtcgtgc
     1981 cataagctgg caaatcgcgt cctgcttgcg cccacatgac ggagtgcgtg gaatgagtat
     2041 gagtaatagc agttatatct tcaaaagttt gatacaaaac aacatgtgtg gggagatctg
     2101 aagatggttt taagctatct tcttctatta tatttcccga caaatctgtt accaccattt
     2161 gatctgcttg catgcattca tatctgacac cgcttggctt aatgacgatg actcctagct
     2221 cacggtccac ttcactcaca ttgccccaag tcaatttgac taatccgtat cgcggcaatg
     2281 ccagatttgc ttcaaggaca cgttgcttca ttgctgcaat tttttcttca caagacatca
     2341 tagccagcct ccttcaattt agaacttaag tagtcttttg cctgctggat ttctcttcga
     2401 aagtcttggc ttttttcact ccacatttca atcagaaatg ttccatcata atctagacgt
     2461 ttcaaatgtc ttaataatcc aagaaaatca acacaaccgc taccaaaagg aacatcacga
     2521 aactggcctg aatattcttt tgttacaggg taagtatctt ttaagtggat agcagtgata
     2581 taatcgatcc ctttctcaag ttctcgagcc ggattatttt ccggccatgc ggacaaattg
     2641 cctaggtctg gatatacttg tagaaaaggt gaatgaatct gttctttgat ttctaaaaat
     2701 tttgaaatgg agttcataaa tggatcatcc atgatttcca cggaaagaat gatgccatat
     2761 ttagaagctt cctttacgct ttcttttatt ccttcgataa agtcttctct ggaattcatt
     2821 gatttctctt cataataaac atcgtatcca gctatttgga ttactttgac gcttagatta
     2881 tgcgccaaat ggattgcttt tttcattagt tgtttcgctg cttttttctt ttctggatca
     2941 gcagaaccaa acggaaatcg ccgatgcgca cttaaacaaa tcgaatggat acgtacaccg
     3001 gagctgaaca tctcttctct cagctcagct atcttttctt cagaccaatc taatcgtgct
     3061 aaccgctcat ccgtctcatc aatcgacatc tcgataaaat cgaagtccat ttcttttaca
     3121 agggaaaaac gttctttcca tgaaatatct ttgggtaacg ctttttcata aatcccaatt
     3181 tgagccattt ccttaccccc agatacgaat gatctcatct ttgaagtttt gtgcggcctt
     3241 catcggatca tttgctgctg taataccacg accagtgata aaggtataca catcgatccc
     3301 ttcaaataag t
//
LOCUS       DACEUZ010000171         3294 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.171, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000171 DACEUZ010000000
VERSION     DACEUZ010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3294
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.171"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            134..1102
                     /locus_tag="IXG44_002468"
     CDS             134..1102
                     /locus_tag="IXG44_002468"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HAQ1659161.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(1419..1943)
                     /locus_tag="IXG44_002469"
     CDS             complement(1419..1943)
                     /locus_tag="IXG44_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HAQ1659162.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            2230..>3294
                     /gene="scm"
                     /locus_tag="IXG44_002470"
     CDS             2230..>3294
                     /gene="scm"
                     /locus_tag="IXG44_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAQ1659163.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSS"
BASE COUNT         1048 a          667 c          611 g          968 t
ORIGIN      
        1 atgaacgccc accctcttct tttttctctt tccttctttc gtttttgctt tatgtatcaa
       61 ccgttgtttc ttcgaaaact attttgttct ctccttcttc ctgatagaat ggatgggaaa
      121 ggagcttgat gccatgacaa cgaaagacaa ggtacttcaa ttattgaaaa agacaccaga
      181 ttttctttcc ggagaaaagt tggcacagca actggaagtt tcccggacaa gtatttggaa
      241 agccatcaaa gaattggaaa aggcaggcta tcgttttgag cacgggccta cagggtatcg
      301 ttacctgccc tctgacgtac tagatgcaaa agaaatccag caagagctac ggtcgtttat
      361 tcctgatttg aaagtacaaa cgatgttgac ttctgaatct acgatgaaag atgccaaact
      421 ggcagcagct caaggagatt ctgtttcgaa gttatttgtt gctgatacac aagaacaagc
      481 tcgtggacgt tttggacggc cttttttcgc tgaacctgga cgaggaatct acatgagtct
      541 cttgctccat ccagacaaac ggtttgatga attgcctcaa tatacgatca tcgcagccag
      601 tgcctgtgtg aaagccattg aatctgtaac cggtcgttct gcttcgatca aatgggtcaa
      661 tgatatctac atggatggaa aaaaagtctg tgggatttta tctgaagcag tcagtgatat
      721 ggaaagcggc aggatcagtc atatcatcat tgggatcggg cttaattttt ctgttcctca
      781 aaatcatttt ccagctgatc tccaagaaaa agccatctct gtctttcccg acggacaagc
      841 tagtgtcaca aggaatcagc taatcagcaa aatctggcaa gaattgttca cttatctttc
      901 tcacccagaa aaagcgttgg acttctataa agaaaaatca ttcgtattgg gaaaaaccgt
      961 gtcgttcaat caaaaaggaa cgacttatgt cggtcttgca aaagaaatcg gtgatcatgg
     1021 cgaactgatc gtccaaacaa atgaacagaa gatgagtttg tcttctggcg aaatcagtct
     1081 cacatctatc cgtacgcaat aattatcaga tttcacaaaa ggacacctga tctcatacag
     1141 tgtccttttt tctgaattct cttcaaaaga ctctccttat tctatcagct gacagtttct
     1201 ctatcctcta gaaaaaggta ttctaaaaat atctataaag catggtacac tttctttaaa
     1261 tgaatcaatg caaaaaacaa gagagtcatc agtttacacg atcaaaagag atagcaggcc
     1321 gcttcctttt tcaatcttat agatagacca tgggtaacga taaaaacaaa aaagaacttg
     1381 cgacttattt tgccgtaagt tcttttttag ctagtgcctt aatctcttaa tcccaacaaa
     1441 cgtctcgcat agatcacctg accagagtgc atagcagcat catcaataat agaaacgaga
     1501 cgcattcctc gtgtgactgg tggtgtccag ttctcgtcaa ttacttcatc tagctcggaa
     1561 ggttccagcc ttcttaagta agaaatagca aaatcagcag ctcctcgcaa gtatcctagc
     1621 agatcttcta tttgattagc ttggatctgc tttgcttgtt ccagactatg cttccaatct
     1681 tgttcgttat ctgctatcgt catcggaaac cggctttccc agtcttgact atgccaaatt
     1741 ggagaagttc ctgctaaatc agcaatctgg aaatctaatt ctcgagcggt atgccaagcc
     1801 agccaagtca ttgatttgat ctgcggggct ctatcttcca ctggaaaacg attggcctcc
     1861 tcaatagaga cgccttcaaa caaacgttca aaccgatcgt atgcccgatc cacactctcg
     1921 catagcatat ctaaataatc catttatttt cctccttttt atttatccta cttacttttt
     1981 cctaattcta ggatacctac ttatatgtta ctacacaccc ttatttttta cactttaaca
     2041 gtcaccatca tcaatagttt ggagattgaa agaaaaagat agtgtcgaac tatcaattga
     2101 acaagaagca acaatatgaa cgtttcatta atattcttta aataaacaat gatttttcaa
     2161 acgaacagtt taagactagg agaaaaagag tataatagga aaatgtttga aaggaaagga
     2221 aggggaaaaa tgaaaaaaag ggcaagttta ctagtcctag ttgctttgtt gttcaatatc
     2281 ttttcgtatg gtgtggccgt ttcagcggaa acatacattc ctaaagaccc tgggaatatt
     2341 tataatgatc taactggtaa ctatggcttg ttaggaattg ctagtcaatt ccacgtattt
     2401 gctaaaaata agacaactgt gaatgcacat acagacggaa atatagcaac acatgaactt
     2461 gatgcaaaaa ataattttgg tacagagctt catgaaggtt tattgaacca agaaataaat
     2521 tatatacaaa aaactgattc tttcgctaat tcttcaggca tacctacagg tagcacaaga
     2581 atgaataaat ttgtcgttgg cagtaacgtg gatgtagcac ttcaagatgg tcatcctaca
     2641 gtcaatggat ccaggcttga tcacttaact ccagatgatt tttataaaga tcgtgctggg
     2701 catgaatata ttgatttcga gcaggaattt gctaaattga atgtagcagc aaatgatctt
     2761 gcaacgatca ctccagctaa aacatatact gctgcagatt ttccagacat gaacaatcgg
     2821 acaatcgatt taactgattt atcagatacc gggtttttat tggtaaatat cgatgcagaa
     2881 gtgttaacga tgaatacgcc acttcaaatc attaatccaa atgatcaagt agttgtattt
     2941 aatgtcatca acagttcttc tgcattgaac gtacagtcac caatcaaata caatgatcgt
     3001 tcaaatcatg aaacagaaga tttttcggat gcgaatatct cttggaattt tggaaatgag
     3061 atgactgatt tgacaatcag tgctcctttt caaggaacaa tccttgcgcc taatgctact
     3121 atccgcatta ctcaaaacca agacggaacg attataggaa caaatgtgat tttagatgca
     3181 gctaccaatc gttgggatcc taacgaaata tttatcagcg acaatggtac agatacgaca
     3241 gacacaagcg acagtacaga tacatctacc acaagtgata gctcagattc atca
//
LOCUS       DACEUZ010000172         3270 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.172, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000172 DACEUZ010000000
VERSION     DACEUZ010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3270
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.172"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            <1..238
                     /locus_tag="IXG44_002471"
     CDS             <1..238
                     /locus_tag="IXG44_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1659164.1"
                     /translation="SNQPRTVEVRETRAEASQGDKTQKINIIEERKERPNEKVIVEQE
                     VKEKKRPRFTYENNPLFQDISDKPNPLFDSLFKK"
     gene            252..440
                     /locus_tag="IXG44_002472"
     CDS             252..440
                     /locus_tag="IXG44_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1659165.1"
                     /translation="MQLILQISTQDFLELAELCDSVKKATNEKITVTNYIELMIQEEL
                     MMKNEIESNFKCCSLEKM"
     gene            452..1063
                     /locus_tag="IXG44_002473"
     CDS             452..1063
                     /locus_tag="IXG44_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HAQ1659166.1"
                     /translation="MDDKQKMYEKNRKHSEALNFRVDKKTYLELTLIAKQRGISRADV
                     CRERLKGLKYEPPLLAEDDRVALIKQLNQIGNNLNQAVKALNIIAKYFREQSENKKEK
                     SLHFSFDVSRYNIFKDELANSIAKKMMISYPDDIFLESNRSTSSFLKLSEVEKDYFQY
                     WLEKQTRPDTKEEYSQKVDRNLIHSIQIIASLKDEVKQLWELL"
     gene            1048..2439
                     /locus_tag="IXG44_002474"
     CDS             1048..2439
                     /locus_tag="IXG44_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAQ1659167.1"
                     /translation="MGATITSKGRKKNLSSLLQYAANPSKKEKCVAVSGINCSDNSEE
                     ALQEMNLVRTRMNKSEDIAQAFHVIHSFDKNISKELSIEKMHEIAVEFAEKAFPNSQI
                     IVASHNDKDHFHSHLVINNIDMETCKRLRIDPKDLERMRKINDEVLVENGLEPVDNQY
                     YENLEKRTNLYSNDPSKLKNGKINYQEFVQNAIEATLENCEVNSFVRFSDHLAKEYNI
                     EVYQFSKTNNKLGYVLYKQDVNFYERRDELITLGREKQSRKEKSQYVERTFSARKLGK
                     KYSFESVFERLEKNLEETHMNNFVDLDTEQIIALASGVDYKTGELNVPFVELDSIAQK
                     QFSDHEGAKKIIKYDDFLREVDRHYANTISLEDDQEYTIKLHTDVNPRTLRSTKSKTI
                     LVKKTDQKELFLPAEGFKNCSHTPSAKQIHYQISRIMNDDVKMYRAKKDYEFTQQMIE
                     NQQRRQRDFEMDF"
     gene            2655..2957
                     /locus_tag="IXG44_002475"
     CDS             2655..2957
                     /locus_tag="IXG44_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1659168.1"
                     /translation="MSDPYIKVYCSKETKTIFDSFYEQNKGIFGTKANMFRVMVSNLP
                     VLANQSNVKLSNPESKGIEQKISEIESIVVGKLDEIDQKLSYYLKNKCKTEEGKDV"
     gene            2950..>3270
                     /locus_tag="IXG44_002476"
     CDS             2950..>3270
                     /locus_tag="IXG44_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1659169.1"
                     /translation="MFKVYFPFQWLFKGILLLLLGLILFGSYLTIKARYFPEEKPVKI
                     VTKDLKMGETYQADGVEITFKKVELTEKRNEFAQHDRVLQVTYTMKNDTENPIRPQQW
                     SIENK"
BASE COUNT         1287 a          456 c          634 g          893 t
ORIGIN      
        1 gtcaaatcaa ccaagaacgg tagaagtacg tgaaacaaga gctgaagcaa gtcagggaga
       61 taagacgcaa aaaataaata ttatcgagga acgaaaagaa aggcctaacg aaaaagtgat
      121 tgtagaacaa gaagtgaagg agaagaaacg tccacgattt acttatgaaa ataatccact
      181 gtttcaggat atttctgata aaccaaatcc gctgtttgat agtctattta aaaaataaag
      241 aggtgattgt catgcaatta attctacaga ttagtacgca ggatttctta gaacttgccg
      301 aattatgcga ctcagtaaag aaagcgacaa atgaaaaaat cacggtcaca aattatattg
      361 aactgatgat tcaagaagaa ttaatgatga aaaatgaaat agagagcaat ttcaaatgtt
      421 gctctctaga aaaaatgtga ggggataatc aatggatgat aaacaaaaaa tgtacgaaaa
      481 aaatcgcaaa catagtgagg ccttaaattt tcgtgtcgat aaaaagactt atcttgaact
      541 gacattgatt gcaaaacaaa gaggaatttc tagagcagat gtttgtcgtg agcggttaaa
      601 gggactcaaa tatgaaccgc cgttattggc tgaagatgat agggtagctc taatcaaaca
      661 gttaaatcaa attggtaaca atttgaatca agcggtaaaa gcattaaata tcattgctaa
      721 atattttaga gaacaatctg aaaataagaa agaaaagagc ctgcatttta gttttgacgt
      781 tagccgttac aacattttta aagatgaact tgcgaatagc atagcaaaaa aaatgatgat
      841 tagttatcca gatgacattt ttttggagtc caatcggagt acatcttctt ttctcaaact
      901 gtcagaagta gaaaaagatt attttcaata ttggttagaa aagcaaacta gacccgatac
      961 gaaagaagag tattcccaaa aagttgaccg gaatttaatt cactcgattc aaattattgc
     1021 aagtttaaaa gatgaggtga aacaactttg ggagctacta taacatcgaa aggacgcaag
     1081 aaaaatttat cctcgctatt acaatatgca gctaatcctt caaaaaaaga aaaatgcgtt
     1141 gcagtctcag gaataaattg tagtgataat agtgaggaag cattgcaaga aatgaattta
     1201 gtaagaacca ggatgaataa atctgaagat atagcacaag catttcatgt tatccattcg
     1261 tttgataaga atatttctaa agagttaagt attgagaaaa tgcacgaaat agctgtcgaa
     1321 tttgcagaaa aagcttttcc aaattcgcaa attattgttg caagtcacaa tgataaagat
     1381 cattttcata gtcatcttgt tattaataat attgatatgg agacctgtaa gaggttgcgt
     1441 attgacccta aagatttaga acgaatgcga aaaattaacg atgaggtgct tgttgaaaat
     1501 ggactcgaac ctgtagacaa tcagtactat gaaaatttag aaaagagaac gaatctctat
     1561 tcaaacgatc caagtaaact gaaaaatgga aaaattaatt atcaagagtt tgtacaaaat
     1621 gcgattgaag ctacgttgga gaattgtgaa gtaaattcat ttgtgcgttt ttcggatcat
     1681 ttagcaaaag aatacaacat cgaagtttat caattttcaa aaacaaataa caaacttggc
     1741 tatgttcttt acaaacaaga tgtaaatttc tatgagcgta gagatgagct aattacttta
     1801 ggaagagaaa aacaatctag aaaagaaaaa agtcagtatg tcgaacgtac ttttagtgca
     1861 agaaagttag ggaaaaaata ttcatttgaa tctgtttttg aacgacttga aaagaattta
     1921 gaagaaacac atatgaataa ttttgttgat ctagatacgg aacaaattat agcgttagct
     1981 agtggagttg attataaaac tggggagtta aatgtaccgt ttgtggaatt agattccatt
     2041 gcacaaaagc agtttagtga tcatgagggt gctaaaaaaa tcattaaata tgatgatttc
     2101 ttgcgagaag tagacaggca ttatgcaaac acaatatctt tagaagatga tcaagaatac
     2161 acgattaaac ttcatactga tgtgaatcct agaactttac gtagtactaa aagtaaaaca
     2221 attttagtta aaaagacaga tcagaaagaa ttatttctac cagctgaggg ctttaaaaat
     2281 tgttctcata caccttctgc taaacagatt cactatcaaa tcagtcgcat aatgaatgac
     2341 gatgtgaaaa tgtatcgtgc taaaaaagat tacgagttca cgcaacaaat gattgaaaat
     2401 cagcaaagga gacaacgaga ttttgagatg gatttctaaa ggaattagcc agtagacttt
     2461 ccagtttccc atcgatacgg agggaattgg aaagaggcgg ttttgagggc atacgccagc
     2521 tacccatcta cttgccagtc aatccgccct tgcttacgca gggctacttt ctggcaagtt
     2581 agatggggaa aacctctgag ggagcaagct ccctacagac tccctaaata aaaaaagata
     2641 aggagtaaaa acacatgtct gatccgtata ttaaagtgta ttgttcaaaa gaaactaaaa
     2701 caatttttga cagtttttac gaacagaaca aagggatttt cggaacaaaa gccaatatgt
     2761 ttcgtgtgat ggtatcaaat ttaccagtgt tagctaacca atcaaatgta aaattaagta
     2821 atcctgaaag caaaggaatt gaacaaaaaa tttctgaaat agaatcaatt gtggtgggaa
     2881 agttggatga aattgatcaa aagttatcgt attatctaaa aaacaaatgc aaaacggagg
     2941 aggggaaaga tgtttaaagt gtattttccg tttcagtggc tctttaaagg aattctttta
     3001 ttgctactag gattgattct ttttggaagc tatctaacaa ttaaagccag atattttcca
     3061 gaagaaaaac cagtaaaaat tgtaactaaa gatttaaaaa tgggtgaaac ttatcaggca
     3121 gatggggtcg aaataacgtt taaaaaggtt gaattgaccg aaaaaagaaa tgaatttgcg
     3181 caacatgatc gtgtattaca agtgacctat acaatgaaaa atgatacaga aaatccgata
     3241 cgtccgcaac aatggtcgat tgaaaataaa
//
LOCUS       DACEUZ010000173         3266 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.173, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000173 DACEUZ010000000
VERSION     DACEUZ010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3266
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.173"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            484..771
                     /locus_tag="IXG44_002477"
     CDS             484..771
                     /locus_tag="IXG44_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1659170.1"
                     /translation="MEIKEIKVRGINKKYVQEIDHRCVELTEQTGQKWSRNDYLKLLI
                     ENDFERPLMDYKKDQFDRLLEKFTDVQLHNTKVLEAYTNEVKNLIEILIAH"
     gene            789..1211
                     /locus_tag="IXG44_002478"
     CDS             789..1211
                     /locus_tag="IXG44_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1659171.1"
                     /translation="MVKAIEKHITFYEAHDEVLKHIMETQPTVKNYSEAIRFLCMNYD
                     PEIEKNNNMTNAKLNAMGKEISILIEMVSEFADLHLKDTGILIGTNSTVYQEAKERVE
                     GKIKANQTKKYSPKIKKSINESVHARVDPFDPTKFLKR"
     gene            1231..2823
                     /locus_tag="IXG44_002479"
     CDS             1231..2823
                     /locus_tag="IXG44_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1659172.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEMERRTPEE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMGNGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQSEQKEYQRLNQAIKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVVSHDNSYLEKIGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEVENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPDKRSEWHYGDEKRTKLSPYTREKLDELTKYVLTKEPEYQEYIQ
                     ISKALKEEAKSLYGESKRESKDAEKNALFDLKKRTGNAILTELKKQNSQIKPLIESMD
                     QMQSIYNERSFRTLKQLPTNEYKYSYEEFMKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQIEQERQ"
     gene            3079..>3266
                     /locus_tag="IXG44_002480"
     CDS             3079..>3266
                     /locus_tag="IXG44_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1659173.1"
                     /translation="MAYSTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQNGDI
                     LTLNLTTKTFVDAAEDYP"
BASE COUNT         1310 a          500 c          628 g          828 t
ORIGIN      
        1 gaaaaaagta tataaaaaat gtttatggat cagctatatc accctaggta tagctgttaa
       61 agagatggga gaaaacatta accacgattc taacacaaac atcaagcaca taaaacattt
      121 aacactcctc ctttctggct gaagatttca accaaacagg aagacagcgt atcaattata
      181 gcatacactt tttcttaaat tcatttttca tatgacaaaa tatttccttc taatcgacaa
      241 agttagaagg attttttatt gtctaaatac acaaataacc ttaattttta aagagcataa
      301 ggaaacgtgg gaacactttt taaaaaaagt gttccaatag gctgtgccta ttacgtttct
      361 ctaaaacaaa agtgggtaca aaatattcga tttatggata gtgtatggat agtaaaacag
      421 ctatacatgg atagcatatg gatagactcc atagactatc catacttttt tcggagggtg
      481 aaaatggaaa ttaaagagat taaagtaaga ggtattaata aaaaatatgt tcaagaaatt
      541 gatcatagat gcgtagaatt aacagaacaa actggtcaaa aatggagtcg aaatgattat
      601 ctaaaactat tgattgaaaa cgactttgaa cgtcctttaa tggactacaa gaaagatcaa
      661 tttgatcgtt tattggaaaa atttacagat gttcagctgc ataatacaaa agttttggaa
      721 gcatacacga acgaggtcaa gaacttaatt gaaatattaa tcgcacatta ggaaggagat
      781 aatgttctat ggttaaagcg atagaaaagc atattacatt ttatgaagcg cacgatgaag
      841 ttttaaaaca tattatggaa actcagccta cggtaaaaaa ctattctgaa gccattcgat
      901 ttttatgtat gaattacgat ccagaaattg aaaaaaataa taatatgacg aacgcaaagt
      961 tgaacgcaat gggaaaggaa atttctattt taatagaaat ggtttcagag tttgcggatc
     1021 ttcatttgaa agatacagga atattaattg gtacaaattc aactgtctat caagaagcaa
     1081 aagagcgtgt ggaagggaaa ataaaagcga atcaaacgaa gaagtattct cctaaaatca
     1141 agaaatcaat aaatgaatct gttcatgcca gagtagaccc gtttgatcca acaaagtttt
     1201 taaaaagata aaaagtagaa ggggaatatg atgaataaat cgcctgcagt cattctcact
     1261 tgtcaattta ccttgcccaa taaaaaatct ttttcaacat acattgatta tatgacaaga
     1321 gaaaaagcgc tggaagaaat ggaacgacgt acgcctgaag aagaacagga actgcaacga
     1381 atcaagaatg ctttagagga tttttatatt cctgaaggag aaactttttc ggagagtaag
     1441 acggataaag aattcagcga aacgtcgaat gaagctcaat ctttgatggg aaatggaatc
     1501 aactttgaca agttagagga acaagatttt accaaatatc tttcttatat gacccgtcac
     1561 tatgcgttag aacaaaagaa agaactgacg caaagtgaac aaaaagaata ccaacgcttg
     1621 aaccaagcaa taaaaaaata tcaaacagag ccacacaaaa aagcttcagg aaaccttcct
     1681 ggagtttttt ctatggcttc agacgaagtg aaaggaagtg atctgaagga gattaagcaa
     1741 atcttccaaa gaggacaaca aaaaggctca atcatttatc aagatgttgt ctctcatgat
     1801 aattcgtatc ttgaaaaaat aggattatac gatcccaaaa cagatacttt agatgaagaa
     1861 ggattaaaag cagctggaag aaaaatgatg gagacattat tcgagaaaga gcaactgaac
     1921 gaaacaggct actggatggc aacgattcac cgtaatacca aacatatcca tattcatttt
     1981 gcagttgttg aaaaagaaaa tacgcgtaaa ccttacacgg aggttgaaaa tggtgtgagt
     2041 tatatcgtac cgaaaggtaa gagaagacaa gcaacattag acgcaatgaa gaccaattat
     2101 gtacatgagt tagaacgttt ttcttatgaa aaggatcgaa tgattggtat ggagaaacga
     2161 aatttattga cgcggaaaag tgatttgcgt aacaccttaa caaaagtggt aaaagagcca
     2221 gcgcgttacg atcgacacgc aatgaactta ttaaatgaag tatataaaac cctacctgac
     2281 aaacgttcgg aatggcatta tggggatgaa aaacgaacca aactttcacc atacacaaga
     2341 gagaaactcg atgagttaac caaatatgtt ttaacaaaag aacctgaata tcaagaatat
     2401 atacagattt caaaagcatt gaaagaagaa gcaaaaagtc tttatgggga aagcaaaaga
     2461 gaatcaaagg atgcagaaaa aaatgctctc tttgatttga aaaagcgcac aggaaatgcc
     2521 attctaacag aactgaagaa acagaatagt caaataaaac ctttaataga aagtatggat
     2581 cagatgcaat ctatttacaa tgaacgatcc tttcgaacgc ttaaacagtt accaacgaat
     2641 gaatacaaat atagttacga agaattcatg aagcgcagga aacaattcca acaaagagtt
     2701 gtcacgcaac ggagtttgcg tcaattaaat cgaacgatta atgcccatga agaaaaatac
     2761 cacgcaagta aagcctacga agagcaacaa cgaagaatcc aaatagaaca agaaagacag
     2821 taaagacagt aaagttagcg ccttattata ctacttggga aatgaatgaa gaggggatca
     2881 ataacttccc gtgtaaaagc attgaagagt ctatttcact cacagaatgt tagttgttaa
     2941 gtgaataaaa gaagtaaaag agtcctgaac gtaattgtcc aggactcttt ttttcaatga
     3001 attgttttcc gccaattcaa tgagtggtgc gatttgcgac tacattatat caaaagaaag
     3061 agaagggagg aagaagaaat ggcttattca acgattggag ttgttgttga aaaaagcaga
     3121 gacaatctcg tttttgtgac agaaattcaa acaggcagag cttttgtcgt cacagacaaa
     3181 gcggcaaaag cgtatcaaaa tggcgatatt ttaacgttaa atctgactac gaagacgttc
     3241 gtcgatgcgg cagaagatta tccatt
//
LOCUS       DACEUZ010000174         3234 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.174, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000174 DACEUZ010000000
VERSION     DACEUZ010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3234
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.174"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            183..1137
                     /locus_tag="IXG44_002481"
                     /pseudo
     CDS             183..1137
                     /locus_tag="IXG44_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 105 protein"
     gene            1154..2178
                     /locus_tag="IXG44_002482"
                     /pseudo
     CDS             1154..2178
                     /locus_tag="IXG44_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
     gene            2184..2663
                     /locus_tag="IXG44_002483"
     CDS             2184..2663
                     /locus_tag="IXG44_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HAQ1659174.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKARLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            2679..>3234
                     /locus_tag="IXG44_002484"
     CDS             2679..>3234
                     /locus_tag="IXG44_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HAQ1659175.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGA"
BASE COUNT          979 a          540 c          751 g          964 t
ORIGIN      
        1 cttggaaaac gttgtttctc caagttcttt ttttaatttc caagtaactg aaagttactt
       61 ttgttattta acatttaggt caattttaga cagtttttgt tcaaaaagcg atatttatag
      121 tggacggtat tactgatata ttaaggatat aaaatgaaag cgtttaaaaa ggtggcgtaa
      181 agatgattat taaagaagag atttttgtac ctaaagaatt agtgaaagaa aaaattgacc
      241 tgctcgttga aaacctgaca acgatcaaag acgataatgg tgaatttttg ttgggctttg
      301 atggactgaa agtagatgac aagagctgga cagtgtggaa ttggccacaa ggtgtcggtc
      361 tatatggtat ttataaaaat taccagatga caaaaaatcc tcgcgcttat caagtggtca
      421 atgaatggtt tgaggatcgt atggaagaag gcgctccgcc caaaaacgtc aatacaatgg
      481 caccgctatt gactatggct tacttgtatg aagacacgaa agattcacgc tatttgcctt
      541 atttagaaca atgggcagaa tgggtcatga atgacatgcc tcggacaaat gaagacgggt
      601 tacagcatgc cacatatgga ccggaaaata agaatcagtt gtgggacgat acattgatga
      661 tgacagtgct tcctttggca aaaatcggga aactgctggt tgacgattgc tattccagag
      721 atcattgaaa tcttagaatt atcgaaagaa gatgcattgc gcaaattatt gatcgaaaca
      781 cttgaagcac aagtgcgtgc attaaaaact tatcagtctg aaagcggttt atggcatacg
      841 ttgttggatg atccttcttc ttacgtagaa tcttctgcaa ctgcaggatt tgcttacggt
      901 attttgaaag cggtccataa acgttattta ccacaagaat ataaagaagt cgcttatcgt
      961 gctatccaag gattgcttga acaaatcgat gaaaaaggag aagttcaaaa cgtttcaatc
     1021 gggacaggaa tgggggacag tttagatttt tatcggaata tcggaatcac agcaatgcct
     1081 tatggacagt cgttaacggt gctttgtttg acagaattgc tcgtctctta ttgttaaaag
     1141 aaaggaaacc catatgaaaa aaacatcact gctgtttgca gcaagttcgc ttagcctagg
     1201 tctgctaggt ctaagcggct gcggaaataa ttctagtgtg gcaactgtcg cagatgcaga
     1261 cgtaaatgag atagttacct tgcgctttgc ttatgcaagc aacagccagt aattgatgca
     1321 atgaatgaat ttgggcgttt agtaaaagaa aaaacagatg gttccgtaca gatccagtat
     1381 ttcccagatg gacaactggg tggagaacgt gaactgatcg aattgacaca gtcaggagca
     1441 gtagatttca caaaagtaag tgcctcagcg ttggaaagtt tctctaaaga ttattcgatt
     1501 ttttccgttc cttatctatt caatgatgaa gaacatttct ttaaggtgat ggataatgaa
     1561 gaaataatgg agccaatcta ccaatcgacc aaaaatttag gatttaccgg attgacctat
     1621 tatgattctg gtcagctcag cttttatatg gtagacggtc cgatccatac gccggaagat
     1681 ttgaagggaa agaagatccg tgtgatgcaa agcgaaaccg ctatcaaaat ggtagagctt
     1741 ttaggcggtt cgcccgtacc tatgggaagt gatgaagtgt acacgtctct tcaatccaat
     1801 ttgatcaacg gatcagaaaa caatgaattt gtcttgtata cagctggaca tggcggggta
     1861 gctaaatact attcctatga tgagcataca cgtgtgccag atatcatcat catgaatgat
     1921 gccatcaaag aacagctaac tgatgaacag gaaaaagcca ttgaagaagc agcgaaagaa
     1981 tcaacggttt ttgaaattga agcttttgca aaagcaattg aagaagaaaa agctttagca
     2041 caagaagaac atggagtcca attcaatgat gtagataata caccgtttcg agaggcagtt
     2101 gccccacttc atgatatatt caaaaacgat cctacctaca aagaccttta tcaaaagatc
     2161 caagagcaag gagcgtagct gtaatgaaaa tgattttaaa caaggtatta gagatcctgg
     2221 gagctgtttt gcttgttggg atggtcgctg ttgttttatg gcaagtattt tccagaacgg
     2281 ttttaggaaa tccaaatacg gtcacggaag agcttgttcg attcggtctt gtctggtttg
     2341 ccatgttgtc ttccgcgtat gttgtgggac aaaaagcacg tttagccgta acggttttaa
     2401 gtgaaaaatt aactggcaaa aaaagtaaaa ttttagaagt tgttgttcaa agtttatttc
     2461 tggtatttgc tgcgatgatc atggtgtatg gcggatggaa tgctgtcaca ttgacaatgg
     2521 ggcagatctc gccttcttta agtattccta tgggatatgt ttatctgtca gtacctgtat
     2581 cgggggtact catcattatc tatagcctga tcaatctaat cgatacatta agcaacaagc
     2641 cagcaattca agaaaaaata taaaaatgga ggaataaaat ggcaatagaa gccggattag
     2701 tgttattttt tgtatttgtt gtgttgcttg tattagggat gccgattgct ataagtattg
     2761 cattctcatc gatgatgact ttattgttag ttataccgtt tgatgtttct gcttttagtt
     2821 cagctcaaaa aatggtggga agtatcaata gtttttcgct gatcgctatt ccgtttttcg
     2881 ttctctcggg cattatcatg aataatggag ggatagctaa gaaactagtg gattttgcga
     2941 tgttgttcgt cgggcgcgtg ccgggggctt tggctcatac gaatgtgtta gggaatgctt
     3001 tatttggttc catctctagc tcggccattg ccgcttctac cgcgattggt ggagtgttga
     3061 tccctcagca agtggaagaa ggatatgacc ggaagtttgc gacagcggtc aatatcgcgt
     3121 cagctccgac aggtatggtc attcctccaa gtactgcttt tatcatgtat tcacttgttg
     3181 ctggtggtgc ttcgatctct gctttgttta tgggaggtta tctcgtaggc gctt
//
LOCUS       DACEUZ010000175         3217 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.175, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000175 DACEUZ010000000
VERSION     DACEUZ010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3217
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.175"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            50..1438
                     /locus_tag="IXG44_002485"
     CDS             50..1438
                     /locus_tag="IXG44_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-directed DNA polymerase"
                     /protein_id="HAQ1659176.1"
                     /translation="MYKERDDLNYTLIINKDGNYSWRPLVLLHPILYVDLVNCLTKED
                     HWEELKKRFSEFKKDKNINCYSIPVEASKGSKRTDLGETILNWWEYFEQKSLAQNIKY
                     QYCMFTDIADCYPSIYTHSITWAMYGKEKVKNDENKCSGSLGDKIDKKISGMQHNQTN
                     GIPQGSVLMDFVAEIVLGYVDLRLSKELKKLENLKDYKVLRYRDDYRIFSNNRNDLEQ
                     IMKVLSEVLYDLNFKLNSSKTKLSDDIIIDSIKKDKLYWEEIKASIRMNATNLDGDRE
                     IRYSINLQKHLLEIKKLADKHPNSGQLKKALSEFNKERIIKITSKPNDLLPLIGILVD
                     IMINNPGSLPQCVLIIGQLLNFENESKAREIVEMIINKYALKPNTEFMMIWFQRLTTI
                     FGEMFDSVLSSEFVSKIKDPVSNQMFSVDWLNEDAKKSFYEGTIKDEEKYIKMSKKIY
                     ITEFDDFNDYPF"
     gene            1776..>3217
                     /locus_tag="IXG44_002486"
     CDS             1776..>3217
                     /locus_tag="IXG44_002486"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAQ1659177.1"
                     /translation="MTEQIEKNSKSLYQALWNSADILRSKMDANEYKSYLLGLVFYKY
                     LSDNMLRYVSVLLEEETDDLNKAQDLYVEAFKDADIKDDLLYELKDEFSYTIAPALTF
                     TAQVAAIHDGSFQLEDLVQGFRDIEQSSENFENLFEDIDLYSKKLGSTPQKQNKTIAD
                     VMKELEGLNMAGHAGDVLGDAYEYLIGQFASESGKKAGEFYTPQPVAKLMTQIVLQGK
                     EDKKGFSVYDATMGSGSLLLNAKKYSHQPGTISYFGQELNTSTYNLARMNMILHGVPI
                     ANQHLHNADTLDQDWPTEEPTNFDGVLMNPPYSAKWSADKGFLDDPRFSAYGVLAPKS
                     KADFAFLLHGYYHLKDTGVMAIVLPHGVLFRGGAEGKIRKALLENGAIDTVIGLPANI
                     FFNTSIPTTVIILKKDRAKKDVLFIDASQDFEKIKTQNTLRDYHIDAILEAYKTRTIK
                     DKYAYVAEFDEIVENDYNLNIPRYVDTFEE"
BASE COUNT         1182 a          505 c          582 g          948 t
ORIGIN      
        1 ttcaaaaaaa aaccaaccga aagatataaa agatattgta gatacacgaa tgtataaaga
       61 acgtgatgac ttaaactata cgcttattat caataaagat ggaaactatt catggcgtcc
      121 tctcgttcta ctacatccta ttctatatgt agatttggtt aattgtctca ctaaagaaga
      181 ccattgggag gagttaaaaa agaggtttag tgaatttaaa aaagataaaa atattaactg
      241 ttattctata cctgttgaag cttccaaagg ttcaaaaagg acagatcttg gtgaaacaat
      301 actaaactgg tgggaatatt ttgaacaaaa aagcttagct cagaatatta aataccaata
      361 ttgcatgttt actgacatcg ctgattgtta tccatcaatt tatactcact ctataacatg
      421 ggccatgtat ggtaaagaaa aagttaaaaa tgatgaaaat aagtgttcgg gctcacttgg
      481 cgataaaata gataaaaaaa tatctggaat gcaacacaat caaacaaatg gtatccctca
      541 gggttccgtt cttatggatt ttgtagcaga gatcgttcta gggtatgttg atttgagatt
      601 atcgaaagaa ttaaaaaaat tagaaaattt aaaagattac aaagtactca gatatagaga
      661 tgattataga atattttcaa ataatcgtaa tgatttggaa caaattatga aagtgttatc
      721 agaagtctta tatgatttaa attttaagtt aaattctagt aaaacaaaat tatcagatga
      781 tattatcata gattctatca aaaaagataa gctctattgg gaagaaatta aggcaagtat
      841 aagaatgaat gccacaaact tagatggtga tagagaaatt cgttatagta taaatttaca
      901 aaaacattta ttggaaataa aaaagttagc agataagcat cctaattcag gccaattgaa
      961 aaaagcactt tctgagttta ataaagaacg cattattaaa atcacttcaa agccgaacga
     1021 tcttttacca cttattggta tcctggtaga tatcatgata aataacccgg gttctttgcc
     1081 gcagtgtgtc ttaattatag ggcagttatt aaatttcgaa aatgagagta aggctagaga
     1141 aatagttgaa atgattataa acaaatatgc tttaaagcca aatactgaat ttatgatgat
     1201 atggtttcaa agattgacca ccatttttgg agaaatgttt gatagtgttt tatcatccga
     1261 atttgtatct aaaatcaaag atcctgtctc taatcagatg ttttctgtag actggctaaa
     1321 tgaagatgct aagaaatctt tttatgaagg gaccattaaa gacgaagaga aatatattaa
     1381 aatgagtaag aaaatatata ttactgaatt tgatgatttt aatgattatc ctttttaagt
     1441 gaaaatttag aatgaatata aaaaacatta caatctaatt tttttaaaaa aatgttatat
     1501 cggaagtaat ctcttgattt ctatactata agataagaaa accactattt aacaaggtta
     1561 ttcgaacctt attttaagtt acttattatt taatgtatcg aaaaaggtga aataggtttg
     1621 ttataataag aattaagtaa gacatgaatt gtctaagcaa atgtagctta tccatttcat
     1681 taaaatacat atggtgaaaa aacaagttca aatagtaact aattttttaa aaaactgatt
     1741 tataaaaaat acaacacgaa tgaggaaaat aaccaatgac agaacaaatc gaaaaaaatt
     1801 caaaaagctt atatcaggca ctgtggaatt ccgcagatat cctgcgttca aaaatggacg
     1861 ctaatgaata caagagctat ttactaggct tggtctttta taaatactta tccgacaaca
     1921 tgctgcggta tgtctccgtt ctattggagg aagaaacgga cgacctaaat aaagcacaag
     1981 acctttacgt tgaagcattt aaagatgcag atataaagga tgatctcttg tatgaactaa
     2041 aagatgagtt ttcatatacc atcgcacccg cattgacctt taccgcgcaa gtggcagcca
     2101 ttcatgatgg atcttttcaa ttagaagacc tggtacaagg tttccgtgac attgaacaat
     2161 ccagcgaaaa ctttgagaac ttatttgaag acattgatct gtattctaaa aaactggggt
     2221 caaccccaca aaaacaaaat aaaaccatcg cagatgttat gaaagaatta gaagggttaa
     2281 acatggcggg gcatgctggc gatgtcttag gcgatgccta tgagtactta attggccaat
     2341 tcgcatctga atccggtaaa aaagccggtg aattctatac cccacaaccg gtcgcaaaat
     2401 tgatgacaca aattgtttta caaggaaaag aagataaaaa aggcttttct gtttacgacg
     2461 cgaccatggg ttcgggctcg ttacttttga acgctaaaaa atattcccat caaccgggaa
     2521 ccatttctta tttcggacaa gagttgaata cgtctaccta taatttggcg cgtatgaaca
     2581 tgattttgca tggggttccc attgccaacc aacacttgca taatgcggat accttggatc
     2641 aagactggcc aacagaagag ccaacgaact tcgatggggt gctcatgaac ccaccgtatt
     2701 ctgcaaaatg gtcggctgat aaaggcttct tagatgaccc acgcttctct gcctatggcg
     2761 tattagcccc taaatcaaaa gcggactttg ctttcttatt acatggctac tatcatttga
     2821 aagatacggg cgtcatggcc attgttttgc cacatggggt gctctttaga gggggcgcag
     2881 aagggaagat acgcaaagcc ttgttagaaa atggtgccat tgacaccgtc attggcttac
     2941 ctgctaacat cttctttaat acgtccattc caacgaccgt aattatcttg aagaaagacc
     3001 gtgcgaaaaa agatgtgctc tttattgatg cgtcacaaga ttttgaaaaa ataaaaacgc
     3061 aaaatacgct acgagattac catattgacg ctattttaga ggcctataaa acccgtacaa
     3121 ttaaagacaa gtatgcttat gtcgcagaat ttgatgaaat tgtggagaat gattacaact
     3181 taaacattcc ccgttacgtg gatacctttg aagaaga
//
LOCUS       DACEUZ010000176         3133 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.176, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000176 DACEUZ010000000
VERSION     DACEUZ010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3133
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.176"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            208..1041
                     /locus_tag="IXG44_002487"
     CDS             208..1041
                     /locus_tag="IXG44_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HAQ1659178.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(1143..2843)
                     /locus_tag="IXG44_002488"
     CDS             complement(1143..2843)
                     /locus_tag="IXG44_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1659179.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
BASE COUNT         1110 a          520 c          623 g          880 t
ORIGIN      
        1 gaagcagcag tttgaatact gtttagtgga gaggtcgtga aaaagctgcc ctgcaccaag
       61 tgataaaaac agccaaacaa aaaatagctc ctggtccatt gagcttatta ctgaggagtt
      121 agcaggagaa cactatttgc tggagaggtt gtgacaaaaa ttttgtcaca acccttttgt
      181 ttcgaaatga agacaaggag aaaaccaatg aaattcatga cactcaacac gcatagctgg
      241 cttgagccag aaccagagaa aaaattgcaa gaattagcag ataagatcct gttagaagat
      301 tatgaagtga ttgctttaca agaaatcaac caattactgg aatctgaaga agtagaacct
      361 gcgaagctaa tgaagttctg tccggtaaag aatcaagtac ctatccgtaa agataatttt
      421 gcctaccgtt tagttcagct tttaaaggaa cacggaaaag agtatttttg gagttgggaa
      481 atgagccaca tcggttatga taaatatgaa gaaggaaatg cactgcttac gaaaaaaaca
      541 ttggaaagtc aggtccttac cgtctcccaa tttcaaaaga aagaagatta tcagacacgc
      601 aagatcttga ttgggaaaac aaagatagat gatcaggaca tttttgtctc gagctgccac
      661 ttttcttggt ggacagataa aaaaagtggc ttttattttg agtggaaaaa cttggaaaac
      721 tactttttgg aaactcgcgt accacttttt ttcctaggtg atttcaataa tccagtagac
      781 tctcaaggtt attatactgt aagagaaagt tgccttcttc ttcaagattc gtatgttgtc
      841 gcgaatgaaa aaggaaaagc agctactgtg gaaaagaaaa tagatggttg ggaacaaaat
      901 acagaaaaat tgcgtataga ttttattttc gtccctgaag gaatgcaggt taaaaaatat
      961 caacggattt ttgatgggat cgatagtcca atcatcagtg atcactacgg ggtggaaatc
     1021 gaattagatg tgaacgaata aaaaatataa catataacat aaaaaaactg attaaaataa
     1081 aactctccaa acgattatac cgtttggaga gttttttgat cggatgtatt tatccctttt
     1141 gcctaagcac gtaaaagctt gtatctatga taaataaata aacatagcaa ggtaaaccag
     1201 ccaatcaaag taagagaaag aatgaaatag aaatagattg gcgtttgata atgaagcgtg
     1261 agctcgttga ttcctttttg ctgcttcacg attggggtcc cgatatctgt tacttcataa
     1321 tcagtcaatg tttttccatt ttgctgtagc acagtccgat cgtagacgat aacagggata
     1381 tgaacagttt tgtccccagt gtttttccac ttgattgtca aaagaccgcc tggatgtgtt
     1441 tttataaaag ttgtttcgtt ttctagtatt tcttcgttat accggtcgta tttattgtct
     1501 tttgtctcct tatagattgg cagatagtca ggtgtggttt tttggaaaac gaacaagctg
     1561 gtagacatat ctttgctgaa gaatgttttc cttgcttctt ccggcgtatc gaatagataa
     1621 gtatgtcgac tgacaaatgt ttcattttcc cacttttcca gatgctggta taagttctgg
     1681 atcgtctgtg ctgaaaaaat aagaatgcca aatatactta aaagacggaa ccacttttgt
     1741 tcagaccact ggttcagtga aagggcaagg taaagtagaa gcaatagcgt aaatggcacg
     1801 aaaaatcgaa atggaaactg gatcaaacta ataaaaggca catttttttc gatcaatgtc
     1861 gtccatggaa tcaagctagt agaaagaaaa aagaaaacaa gacttgtgta caacacgatt
     1921 ttttggaata aggtgttctt ttgttgaaag aaaataaaaa gcaaaaaacc tgataatatc
     1981 aatataggaa aggccttagg gtaatggagc caataactgc catccagatc tatcgccttt
     2041 tgactcattt ctctgttaac aaagggagga agaatcttat tcccactgta aacaatagct
     2101 aatcctgccc aaatatttgc tgtcagaaca aaataaagca agaccgactt aatcagttga
     2161 agcagtgctt ttcctttttc agtctggtta aaaaagtaat aaagataaaa agggatatac
     2221 atcaaagcta acatgatcga agacagtaaa tggatctgtg tcataagtcc cactgagaca
     2281 gcgactttga taacagggaa ctttttatga aggacgaaat cagcaatagg gatcatacat
     2341 aaaggataaa aacaagtccc ccagctagtg aatccttggt tgatagacca gtattgaata
     2401 gaatacgtac tcatgaataa aacggctact ggtacactta cccaatttcg aacattgaca
     2461 tatctcagta aaatcagcat agaaaatcct gaaataagat aaagaatgaa atttgaaaga
     2521 atctgataat gaaaccagct acctgcaagc aacacgatca atccttgtaa ataagcgaaa
     2581 aatgggccgt aaacggcatt cacgatcctg ccactttgct ggaatccata aagggaagga
     2641 aaataatgga aatccccata tttaatttgt tgcgcggcat catagaaccg attgtaatgg
     2701 aatggggcat cagcacccaa aattagttta tgtgtgaata aatgaggcat gagaaatgcc
     2761 aaagcaatga aaaaaattgc taaaaatggg ccatattttt tcaattgtcg aactcctttg
     2821 caaactttag ataaattcat catatatcct atgcgagagg taggcaagaa attagaaaat
     2881 tcatgaaaat atttatgaat tttacaagaa aaacataagt gaaagttcta aggagagagg
     2941 atgcgacata agttttgttg catctccttg ttttgaaaca gaatttttct taatactcga
     3001 aacaaagtag ctttttcatg acctctcggt aaacggtgtt caatcagctg cttctcggta
     3061 ataagctcac tagcctagaa aatatgagaa gctattttct gactagcgct cttattactc
     3121 aaagcataac gca
//
LOCUS       DACEUZ010000177         3091 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.177, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000177 DACEUZ010000000
VERSION     DACEUZ010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3091
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.177"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(198..1316)
                     /locus_tag="IXG44_002489"
     CDS             complement(198..1316)
                     /locus_tag="IXG44_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1659180.1"
                     /translation="MAKKDKELLTQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            1787..>3091
                     /locus_tag="IXG44_002490"
     CDS             1787..>3091
                     /locus_tag="IXG44_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HAQ1659181.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWGDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
BASE COUNT          969 a          641 c          562 g          919 t
ORIGIN      
        1 caaacaaaaa atatgaagag ctatttttgc ttgctgttct tattacttga tgcagaacag
       61 ctttttcaca acctctcata cacggtgttt aatcagctga ccttcggtat tagctcaaaa
      121 aacggaaaaa tatgtgaaac tatttttccg tttgtttctc taatactcag gtcaaaacac
      181 tgatttcaca acctctttta ataacgaaga atcgctgtca gactcttttc atctggcgca
      241 tctttttgat ccaaaataaa cactttcccg ccattaatga tcacatcatc tgctaatgca
      301 ttcaacactt gacgccgatc atattctgtt tcaggatctt caccgaaacc atcagctaag
      361 tttgacgtag cgataaataa atgtgacact tttccttctt tcgcagctgg aacgatatca
      421 actaactgat cgatgaattt cttatctaag agtttattgt attcttttga ttcttgagct
      481 gcgagttcac ttgacagctt cttcgcccct tcttcgatat ctctaaagga tagctgcgca
      541 ggagaagcag aaatcgcaac gtctgcacaa taatagggat ttttagcaat ttttttgaac
      601 atcgtctgat tttctggtaa tgcaaataaa taaatcggcc attgttcagg attttcgaaa
      661 gaatccttca agtacgtatc aacagcttgg taatagttga cccaatcgat ctctacttct
      721 tcatcttttg gatttacgcc atggaaagtt actccatctc gggctccgcc tcctccatta
      781 gaagagaagt tcaaaccgcc acctgtcagt tcagatccca atgcagtaac gacatcaacc
      841 ggtgcccctt ctggtagaga tacttctttt acttgtttgt tgctgacctc atacaatttc
      901 atggagtcgc ggttcaatcc caataaataa taagaatagt tgaactgccc atttttgata
      961 atagccagta aataaggaag atcacttaca tagtattgat cgtctaccgg gatactcaaa
     1021 cgatggatat aggtctggtc agcagttata atgaccgcta cactcgtagt tgcactacgc
     1081 caaaacgaag gatctcctaa caaattttca attttttctt ggaacgggga ccagtcatgt
     1141 tcggaatatt ttttctcaaa acgtttcttt gcttctttcg caaaattttt gaatttgatc
     1201 tgatcttttt ccacatcttg atgggcgaca tgcgtattca gcatgatcgt gacgaatggt
     1261 cctttagcgc cttctgaagt caattgtgtc aataattctt tatccttttt tgccataaac
     1321 gtacccctcc taaaaaaatc attcctttgt ttctatctaa agtctaccac aacactattt
     1381 ttccatacaa taaaacgtat ctaaaaaaat aaaacggttt ctacggtttt aaatttagct
     1441 atttttttaa gttatcttca taatttcctc aattatttag ccccagtctt caactattct
     1501 tcatgaaatg ctgataaact aagaaaaaga ataaaagagg tggatcttat gcatttgatt
     1561 atgattttca gtttattaag tattatcagt ttatttgctt tcagcgcttc ccgcttatta
     1621 gatgcggaaa cacagcctat tcctgtaaaa gtacgtaaaa acgaccgcta aacttccata
     1681 acgcgcgttc ttatcctaaa gttttttcag aaacattgaa actctctccc ctctctgata
     1741 aaattttaat ttgtagcgaa acaaattaat tacaaggaga tcggatatgt ccataaaaaa
     1801 aacacgtcta gtcctattat tggctagtct cattctttgc cttgcttctt ttctaccagc
     1861 tgttgctgtg caagctgaag atacgttcaa agtaaatgca aaagcagctt ttgctgtaga
     1921 tgcgcaaagc ggtaagattc tttatgatca agatggagaa aaaaccatgg ggatcgcttc
     1981 aatcacaaaa atcatcggtc tttacatcgt ccttgatcaa gtaaaagaag gaaaactgtc
     2041 atggggtgat aaagtttcga tttcagatta tgcagaaaat ttgagtatca ccccagattt
     2101 atcgaatgtt cctttgcata aagaaaacac ctacactgtc aaagaattat tcgactctgc
     2161 aatcatccag tcagccaatg cttcaatggt ggccttggca gaaaaaatct ctggcagcga
     2221 agctaaattc actgaacgaa tgaaagaaca attgaaagat tggggaatca aagatgcaac
     2281 aatcgttaat gcatcaggat tgaacaactc ttatcttggc gaaaaccgac cagaaggcac
     2341 tggagaaaac gacgaaaatc aaatgtctgc tcaagatgtg gccatcgttg cccgccattt
     2401 gatcttggat ttcccagaaa tactggatgt atcaagtact acgacccaaa tgtttggcga
     2461 aaatacgcaa tcaccagtgg aaatggtcaa ctggaactgg atgctcccag gattcgttaa
     2521 ttacaaggaa ggcgtggacg gattaaaaac tggtacgact gagcttgcag gtgcttgttt
     2581 cgttggtacg atcactaaaa acgatcaacg gatcatcact gtcgtattga atgcggacgg
     2641 ccatgcggaa aacccgagtg cccgcttcaa tgaaacagac cgtttgatgg actactgcta
     2701 tgacaattgg tcagaaaaag agctcggaaa agctaacgca agtattccta gtctaaaaac
     2761 cattgatgta aaagacggaa aagaatcaac agttcctgtt gtgcttaaaa gccctgtaaa
     2821 agtatgggta cgaaacgata tggatactgg caaattaacg atcacgccaa cacttgacaa
     2881 gaagcaagtc actgacaatg agatccaagc acctatggat aaaggtacaa aaatcggtac
     2941 cgctacgatc accttggcag acgataaatt aggctattta gaagacaaca atgcaccatc
     3001 aacagagatc attacggata aaacagtgga aaaagcgaat atattcgtga ttggctggcg
     3061 acgcgtcgca gaatttttcg gcaacttgtt t
//
LOCUS       DACEUZ010000178         3086 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.178, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000178 DACEUZ010000000
VERSION     DACEUZ010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3086
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.178"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            451..768
                     /locus_tag="IXG44_002491"
     CDS             451..768
                     /locus_tag="IXG44_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HAQ1659182.1"
                     /translation="MLFYSNFILIVAILLLLNIWIFDRSRNSSIGFRTKRSLSSKKNW
                     VYSQTIFYGGIVLISLLSSTLYSLNIIDVSTSNSISIIGIIIAAIITQLFLVFGEKKR
                     SKK"
     gene            complement(859..1224)
                     /locus_tag="IXG44_002492"
     CDS             complement(859..1224)
                     /locus_tag="IXG44_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1659183.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            1432..1914
                     /locus_tag="IXG44_002493"
     CDS             1432..1914
                     /locus_tag="IXG44_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1659184.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALIVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDLAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            2111..3001
                     /gene="pstS"
                     /locus_tag="IXG44_002494"
     CDS             2111..3001
                     /gene="pstS"
                     /locus_tag="IXG44_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HAQ1659185.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
BASE COUNT         1077 a          464 c          567 g          978 t
ORIGIN      
        1 ttacgcttta aactctttta tatttgaatt cactaagaag cataccatat atttgaattt
       61 actaagaagc ataccatatt tttgaaaaaa agtgagaaaa aaggcttata attaggattt
      121 agagtaatta attagatgtt tattttatag gactgatact atagattaca aatagtatcg
      181 tatttcgcaa tcttaaaatt atcttcttac tgtgaataag ataataaaat ttgtatttgt
      241 cctttttagg taacttattt taatctatat acctttctgg atcaacgaaa gtatacttta
      301 tatagtcata acgccgatgg taactatgca ctgtaacgat aatgctgcta aaatatcttt
      361 ttacgaaaaa catcacaagg aaaaacttaa ctatggtatt atctaaacat aattttttcg
      421 gtatttaata tttaattagg aggtaatatt ttgctttttt atagtaattt tatcttaata
      481 gtagcgatat tattacttct gaatatatgg atttttgata ggagtagaaa ttcaagtata
      541 ggttttagaa ctaaacgaag tttgtcttct aaaaaaaatt gggtatactc acaaactatt
      601 ttttatggag gaattgtttt aattagttta ctttcttcaa cactttattc tttgaatatt
      661 atagatgttt ccacctcaaa ttcaatatct attattggaa tcattattgc tgcaattatt
      721 acacaactgt ttttagtgtt tggagagaag aaaagaagta aaaaatagaa aaactagtga
      781 tagttttggt gatataagaa aaacatatat gtataaaaag aagaaagtag cttctctttg
      841 tacatatatg gtttgttttt atggatgccg agctttttta taagataagg ctaccgccca
      901 taacgaccca aggatttgtg gaactaagta aatacctgca agaagcaaga tcagctgggt
      961 cggttttaaa ggatgataga agtaacgcca ttcagtaaaa ttggcgcaaa ataaatagga
     1021 agtgacagta gatataatat tggcaatgaa taaagatata atacgcctgt tcctttgaaa
     1081 ataatatcca gcaaaaagag ataaaaatat caatgtgatg aacccgctta cggaatgata
     1141 acgaaaatca agcagtaagg cgaaatgcac ataaggaaca aaagataaaa gcaacggaag
     1201 gtttttccag caagttttta tcatctttca tcaccaccta acaagtgtta ttattttgtt
     1261 agttttattg taaattgaat cctgcaataa tcaattactt tttcattatt tctagattaa
     1321 atgaataaac tctcatgcca ttatctctaa aaaaaaagtt tttcccccta atttttttag
     1381 atagaaaatt gtatagtatg atgagagaaa aagtagtttg ggggaacgtt aatgagaaga
     1441 aaagcagtaa tcgatcggct ttatttattt ttttggtttt ttcctatcgt tttgctacct
     1501 tttacatggg taaatgctgt tgtacaaggt gcggagatat taggcctgat ccaaggaagc
     1561 ggatttgtgg tttctaggaa ttttccagct ttgattgttt tctatttagt gataagtgta
     1621 ggtctcttat taccaaaatc tctgatcaat cactacaatt ggcagctgct ttacttttct
     1681 tgcttattag tatttacatc tatgttttct ttgctgcctc gcttgattat cggccctaat
     1741 ggtttgagcg agagtggatt gaaggattta gcttattcct ccatgtattt cttgacgatc
     1801 aaaccatggt tctatttagt aattgcctca tttattcttg caacattttt ttacttctgg
     1861 acagaaatca ggataaaaag tgaacgatct gtatctataa aaaataacta ttgatggtgg
     1921 atcatcagta aaaaaacata tgagctgcgt aaaatgcatg tttctcatat gcttttttta
     1981 ttattatgta aagaatgcga aaaacgttta catgattttt acataactta actatatttt
     2041 tacagcaacc atacgaggga tttacagtcg actgataaac tttagttgta aataagaaag
     2101 gagcaataaa atgaaaaaag tattgtttac tctaggtatg atgggaattc ttttagccgg
     2161 atgcggctca ggatcaggaa atacagatgg ctctgccaca caaaataatt caagtgactc
     2221 aaatgaacaa gtaaaaatcg ttgcggtagg ttcgacagca ttacagccgt tagtcgatgc
     2281 agcacaagaa agctttgtac aagaaaatcc aaattatcaa atctctgtac aaggaggagg
     2341 aagcggaact ggtttgagcc aagtcgaagc tggagcagtt actatcggaa actctgatgt
     2401 atttgcagaa gaaagagacg gagttgatgc ttcaaaatta gtggatcata aagtagcagt
     2461 ggtcgggatg gcaccaatcg tcaataaaga tacagatgtc aaagatatca ccaaacagga
     2521 attgattgat atctttacag gaaaaatcac taactggaag gaagttggcg ggaaagatca
     2581 aaaaatcaac gtcgtgaacc gtgcaaacgg cagcggtacc cgcgcgacat tcgaaaaatg
     2641 gggactggat ggtgctaccc ctgtgcagtc ccaagaacaa gattcatcag gaacagttcg
     2701 gcagctggta agtcaaacac caggagcaat cagctattta gctttctcat atctcgatga
     2761 ttccacacaa gcactaagta tcgacggtgt agaaccaaaa gaagagaatg tagcagataa
     2821 cagctgggga atctggtcat atgaacatat gtacacgaac ggaaaacctt cccctgaagt
     2881 ccaaaaattc ttagactaca tgatgacaga agaaatccaa gaaggacctg tcaaagaatt
     2941 gggctattta ccaatcacaa tgatggaagt agaacgtgat cacgaaggaa atatcaaata
     3001 actagcagat aaacatgcca cggtagtcgt tactattaag gaaaaatcaa ctatgtttcc
     3061 ccacatatac atttttcctt taattg
//
LOCUS       DACEUZ010000179         2970 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.179, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000179 DACEUZ010000000
VERSION     DACEUZ010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2970
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.179"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(84..1031)
                     /locus_tag="IXG44_002495"
     CDS             complement(84..1031)
                     /locus_tag="IXG44_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ1659186.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            1277..1741
                     /locus_tag="IXG44_002496"
     CDS             1277..1741
                     /locus_tag="IXG44_002496"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HAQ1659187.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(1803..2846)
                     /locus_tag="IXG44_002497"
     CDS             complement(1803..2846)
                     /locus_tag="IXG44_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HAQ1659188.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
BASE COUNT          982 a          587 c          567 g          834 t
ORIGIN      
        1 tttattcgga aaaaaggact gtgacaagac ttttgtcaca gtcctttttc atttaaaaaa
       61 atgtttactt catttgacgt atattattgg tgaggcttca attcatttgg cttcactttt
      121 tctgtttctc ttatgatata aacaggacgc tgtttcgttt ctaagaagat tttcccaata
      181 tatttcccga tgattccaag acataacaat tggattccac caatgaacag gaagatcgag
      241 accatggaag gccatcctga tgtaggatca ccaaacaaca atgcccgcaa aataatgaac
      301 aaaattgcaa taccagaacc gacacaagac aaagcgccga taaatgaagc aatcgttaac
      361 ggtgcatccg agaagttcac gataccatca atcgagtagt tgaataattt ccagaaggac
      421 caagatgttt cgccagccac acgttcgcgg ttttcataag aaatatattc tgttttaaat
      481 ccgacccagc taaataatcc ttttgaaaat cggttgtatt ctgttaattc taggacggca
      541 tccaccattt gacgagtcat caagcggaaa tcacgagcgc catcgaccat ttctgtatcg
      601 ctgattttat tgatcaactg gtagaatttt ttcgcaaaga aactgcgtat cgcaggctcg
      661 ccttcacgag tcgaacgccg ggtgccgaca caatcgatat cagaagtagt gatccggttg
      721 atcatctcag gaagcagttc gggcggatct tgcagatcaa catccatcac ggtcactaaa
      781 tctcctgtag aagcttgcaa tccagcgtac agagccgctt ctttcccaaa atttctggaa
      841 aatgagacaa agcggacata aggataagct gaggctagct tcttgattgc tgctaacgta
      901 ccgtcttttg atccgtcgtc aataaacaaa tattcgaact catgggcacc tttgaatttt
      961 tcaacctcat caaaaaaaag aggaacagtt ttttcttcat taaaacatgg gacaacaatt
     1021 gatactttca tcgtttctat cctttcaatc gtatgtttat atatggttta cattcaaaat
     1081 ttaataaacc ttaaataaca cctttaaaat ttgattttac gtaaaagaac aggtattctt
     1141 tttgtaaact aacgtattta gtttaccatg tttcctcggt ttatcaaact tttaagaaca
     1201 atttttatta gttggcaagt agttttggat agagaaaaag gaggaagttc cagtctattg
     1261 actaggagca aacaaaatga aaatacacac acctatcggc tttttatggt tagatgcaga
     1321 tgaatccgga ttgactgctg tatcttttca gccaatttca gaaagcgaat cagcgaatca
     1381 gcgaatttta acgcgcacac aagaacagtt tgaagaatat tttgcgggaa aaagaaaaga
     1441 atttgatgtt cctttatcta taaagagagg tacgcctttt cagcaaaatg tctggaaagc
     1501 tttgtcgacc attccttatg gagaaatccg aacgtataaa gaagtagccg ttgctgttga
     1561 tagtccgaaa gctgtgcgag cgattgggca ggcaaatcga accaatccat tgccaatcgt
     1621 tattccttgt catcgagtga tcgggcaaaa tggaaaactg acaggatata tgggaaacgc
     1681 aatagatggg gttgaaatca aacgccaatt gttgttgtta gaaggatatc tgcaaaatta
     1741 aaaacagttg tgattcggac attgttccat gcgagaatca caactgtttt ttcatgttca
     1801 ttctaaactt tgatcagcca aataaatagt aaagccatca ctgtttggag aagcccgaca
     1861 gctaatccat aagataagaa gcgtgtacct tctttcatga attttgcaaa gtctaaacgc
     1921 agaccaattg ccgctaaagc agtgatctca aaccagctgc tgataaaatg tgccgattgg
     1981 tcaataacta caggtaaaga aaacaagctg ttgaccaaac aaaagatcac aaaccctaat
     2041 acataccaag gaagcggtaa ttttcgtttt ttctgcgtat cagttggcgt ttcaaacaga
     2101 gctggttgtt tcttgtgcat gaatttttca aacagataga caacaacaac gagcatcata
     2161 atacgtagaa tcttaaatag catggcgaat tcgacaacag aattgtcaag taagcttgcc
     2221 ccagcgacta cttgaccaac agattggagt gtaccaccca aaagtgcact tttttcaatg
     2281 agatttccac caaacaactg taatccaaga aaagggagag tcaacatcat cacagtaccc
     2341 agtagattga ctagtgtgat gatctgtcct ttttcttcct ctttcgcatg gatggaaggg
     2401 gcgattgaag caatagcaga agaaccacaa acagcgtttc ctccagccat catcaaagaa
     2461 gaagtatcgc tgaattgcag ttttttacca atcaagtaag caccgatgat agtgactgac
     2521 atttgcagta acacaaaaac aattcctttt acaccaattt gagcaatcgt ttgagctgtg
     2581 acagttgcac ctagtaaaaa aacagagaat tctaatagtt tgctttcagc aaattttgtg
     2641 ccgattgcta gaacaggctg tttaaaaaaa gtatttccta ataaaatacc caataaaata
     2701 gcaatagtcg ctccacctaa ctgtggcaga taaatagcaa gaagcctgct gaaggcagcg
     2761 atggcaaaag aaagcagtaa accgggtaag atttcgcgga ccgctgaatc ctttacagat
     2821 tttattggat gtgacaatga tttcatttga tcggttcctt tcgtcaataa atactaaaag
     2881 catactcgaa catagtagca ctaaaccagt attttgacaa aagaacaaga ggtcgtgaca
     2941 aaagtcttgt acgacctctt gtttcgaata
//
LOCUS       DACEUZ010000180         2964 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.180, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000180 DACEUZ010000000
VERSION     DACEUZ010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2964
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.180"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(58..645)
                     /locus_tag="IXG44_002498"
     CDS             complement(58..645)
                     /locus_tag="IXG44_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HAQ1659189.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(728..1978)
                     /gene="clpX"
                     /locus_tag="IXG44_002499"
     CDS             complement(728..1978)
                     /gene="clpX"
                     /locus_tag="IXG44_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HAQ1659190.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(2225..>2964)
                     /locus_tag="IXG44_002500"
     CDS             complement(2225..>2964)
                     /locus_tag="IXG44_002500"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HAQ1659191.1"
                     /translation="ENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVTFPEDYQAEDL
                     AGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKELVETKEKAADD
                     AKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEMYYQLTGSTEE
                     DLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYNMPIEQIKRVL
                     TEDMLEHDVTMKKAIELITGTATEAKE"
BASE COUNT          771 a          627 c          508 g         1058 t
ORIGIN      
        1 gacttaccag ccaaactttt atgaaagagt ttgactggta agtctttttt attcttttta
       61 tgattcaatt gcttgttcga ttgctgccca cgcttcttct tttccctgtt ttgtaacgga
      121 agaaaagaga ataaacgtgt cattgcgatc aaaatccaac gcttttttga tttcgctctc
      181 atgcttgttc catttaccac gagggatctt atctgcctta gtggcaacaa caattacagg
      241 gatatcataa tacttcaaaa attgatacat ctggatatct tcttgagaag gcttatgacg
      301 caaatctact aaagaaacga ccgcacgtag ctggtctcta gtcgttaaat atgtctcgat
      361 catctttccc cattttgctc gctcagtttt ggatactttt gcatacccat atcccggaac
      421 atcgacaaaa tgtaatgcgt tctcgatcaa ataaaaattc aatgtctggg tctttcctgg
      481 cttaccggaa gtacgggcta aattctttcg atcaatcaat gtgttgataa aagaagattt
      541 ccctacattt gatcgtcctg ccagagctat ttctggcaat tgtgtatctg gatattgttt
      601 tggtgccact gcacttatga taatttcagc ttgatggact ttcatggtct ctctccttaa
      661 caaggtgatg aataaagcaa gcggctgtcg caaaagccaa aatgactttt acggcagcct
      721 cttttcatca acccgctttt ttctctgaat ctttatagat tactgttggc tcaccagaca
      781 attcagctgc ttcttttgtg ataatcactt tttcgatgtt ttcatttgaa gggatatcaa
      841 acatcacatc catcatgatt tcttcaatga tggaacgaag tcctcgagca ccggtattgc
      901 gttcgattgc ttttttcgca atggctctca acgcttcagg ttcgaattca agttcggtat
      961 tatctagaga aagaagtttt tgatattgtt tgactagcgc atttttcggc tcagtcaaga
     1021 tccgtaccag atcatctgtt gtcaatttct ctaaagctgc cataacaggt aatcgtccga
     1081 taaactcagg gatcaatccg aattttagta aatcttcagg aatgatatgc tgcatgacac
     1141 tttcgtcttc agaaagtttt tggttttgtg taccaaatcc aatcgtcttt tcgcccatac
     1201 gattcttcac aatcgtttcg atgccatcaa atgcaccacc tacgatgaac aatatgttcg
     1261 tcgtatcaat ctgaataaat tcttgatgtg gatgtttacg tccgccctga ggtggcacac
     1321 ttgcaacagt tccttctaga attttcagca aggcttgttg gacaccttcg ccggaaacgt
     1381 cacgagtaat agatacattt tcgcttttac gcgcaatttt atcgatctca tcaatataga
     1441 tgatcccttt ttctgcaagt tccacattgt aatcagcaga ttgaagcaat ttaagcaaaa
     1501 tgttttctac atcttccccc acataaccag cttctgtcaa acttgtcgca tctgcgattg
     1561 caaaaggtac attcaatgtt tttgctaatg tttgtgctaa aaatgtttta ccagaccctg
     1621 taggtccgat caagcagata ttgctttttt gcagttcaac atcatctgtg ttttcttgtg
     1681 cttgctgatt gacacgttta tagtgattgt atacggcaac agctaacgag cgttttgcgc
     1741 gatcttgtcc aatcacgtag ttattcaata cttcaagaat ttctagtggt ttagggactt
     1801 cagtgaattc gcgtacagct tcttcataaa attcttcatc aatgatttct ttacataggt
     1861 cgatacattc attacagatg tagacccctg gaccagctac gatctttttc acttcttctt
     1921 gggttttccc gcaaaaagaa cagcgtacgg tctcattgct acttggattg tcatacatgg
     1981 tcttcacccc ttaacttgca aaaagtatgc aagaaactat caaagacttt acagtctttt
     2041 actatcataa catagtttag taaaaagaaa aagtgtttaa caaaggatca actgacctat
     2101 acctttgcat tttttactct taacaaaaaa taaggaactg tgacaaaatc gtgtcacagt
     2161 cccttatttc gaataaacaa ttttctagaa gtagcttctt cagaaatgat ttattacttg
     2221 atttctattc tttcgcttct gtcgctgtac ctgtgatcaa ttcgattgct tttttcattg
     2281 tcacatcatg ttccagcata tcttctgtca acacgcgttt gatttgttcg attggcatgt
     2341 tgtatgtttc tgataattct tggatttctg cattgatttc ttcttctgtt gcttgaagat
     2401 tttcagcagc tgcaattgct tcgatcacaa gatttgttct tgtgcgcatt tctgcttcac
     2461 cttcgaattg tttatgcaag tcttcttctg tagaaccagt taattgatag tacatttcag
     2521 gagagatacc ttgacgttgc atgttgttca agaattcgtc cattgaacga tgtacttcat
     2581 catgaaccat cacgtgtggc aattcaacga tttctgcgtt ttcaacagcc atacggatcg
     2641 ctgcttcatc ttttgcatcg tccgctgctt tttcttttgt ttctactaat tctttgcgat
     2701 atttttcttt caattcgtct aatgttgata cttcgtcatc aacatctttt gcgaattcgt
     2761 catctaattc aggcacttct tttgctttta cttcatggat tgtcactttg aagactgctt
     2821 cttttccagc aagatcttct gcttgataat cttctgggaa agttacattt acatctagac
     2881 tttcacctga tttatgacca actaattgat cttcaaaacc tggaatgaat gagcctgaac
     2941 ctaattcaag tgagtaattt tcgc
//
LOCUS       DACEUZ010000181         2949 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.181, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000181 DACEUZ010000000
VERSION     DACEUZ010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2949
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.181"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(862..1203)
                     /locus_tag="IXG44_002501"
     CDS             complement(862..1203)
                     /locus_tag="IXG44_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAQ1659192.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(1203..1433)
                     /locus_tag="IXG44_002502"
     CDS             complement(1203..1433)
                     /locus_tag="IXG44_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ1659193.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            1720..2058
                     /locus_tag="IXG44_002503"
     CDS             1720..2058
                     /locus_tag="IXG44_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1659194.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(2263..2502)
                     /locus_tag="IXG44_002504"
     CDS             complement(2263..2502)
                     /locus_tag="IXG44_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HAQ1659195.1"
                     /translation="MFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRM
                     PLVLTEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(2478..2825)
                     /locus_tag="IXG44_002505"
     CDS             complement(2478..2825)
                     /locus_tag="IXG44_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF159 family protein"
                     /protein_id="HAQ1659196.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGM
                     KKKTNLCFQIRKK"
BASE COUNT          935 a          531 c          450 g         1033 t
ORIGIN      
        1 atttttactc attcatagac acttccttaa ataactttta ttatagaagg tattgtaaat
       61 aataatattg acaaaaagtg actcaaaata aaaaaaaact tacattatac tggaatgaac
      121 tgtattgata gtgtacctaa aagctctact gacataagat cgtgtaatcg gtaaagtaat
      181 tttattaatt atacgggatg taatcaatta gagaataaac taatcataaa aggtactttt
      241 acattcataa ttgtcgcttt gagattctac tttggttcac tatctgaccc aattgcacat
      301 tttcgatcat tacaagtatt ggtgtcgtta gaacaactaa ataaaaacta gcggtagatt
      361 tttcagaatc tatgctaatt tctgtatgga tgttccagtg atggcaaatt tctcttttca
      421 ttgtttttct tgtcgcatga tatgcctttt tgtgttattc agttaaacta tagaaattct
      481 ttcctagaca gatcttgatc ccaaatttta cgtttttgag catcgatata gtttaaaatg
      541 acattactca tatctaaaga gtttagattt tttaggacta aaactgtcag ttgttctttt
      601 tcttctttct gttaaagtat tgtcgcgact tttcgtatac gttaagatat gtcgtgaaaa
      661 aacaagtttt gtgacacaaa aaaacgaatg acttgaggaa atcacctcaa tcattcgttt
      721 aaaatgctat atattcttta gtttaagata cggaaattaa tgccatcgac cacactagta
      781 gaatgaacga catttacttg gccttttttt aatttcgtta tgtttagcat ataaaaatat
      841 tatagagttt acttacaatg cttaaaacat atactgtata atctgatcaa ttttagccat
      901 gtcttgcaat ggtaagtttt ctattttttt gagttttcgt tccttaaaat caagagattt
      961 taattgtgta ataattactt gtccttttat atctagctct tcaggtaatg agtaacgtgt
     1021 cggtaaattc tttatagtgg aagtaattgg acatatgacc gaaaacattg ttttacggtt
     1081 aaattcataa cgagagacga ctagtcctgg tcgtcttttt tgaatttctt ttcctacgga
     1141 gggatcaaaa tcaatccata caatatcacc ttttttagga atataaattc tttcttctga
     1201 cactataaaa tttctctccc ttcaggagtt aaatcttccc attcgtcttt ctcataatac
     1261 tcggcctctt ccccaccact aaatggatct tcaatttttg gaactaacgt aattgttcca
     1321 tcatcagaat acacaacgat atattcttgg ttttctgaag gtttctttcc gttatcagaa
     1381 ggtaaagtta ccactacaga acttccttgt aatctagatt ttgtaacaag cattatcatc
     1441 actcccttaa taaaagtata ctatataaca ctaagtaacg caaaattccc ctttgtaaac
     1501 tatctagaaa attgttttgt ttaaagtata tcttattgga tataaataga aataaattgt
     1561 agccagttca ttttttcttt aactaaaaaa ataatccaag aaacattgaa cctatggatc
     1621 caaaagctac ccaaatacta tagctaaccc cccgctacct tcaattttgc agataaaacg
     1681 ctttgggaga aaatttcaaa gacaacaggc catcctatca tgcccgatta taaacaaaaa
     1741 gctggctatt ccttttgctc tgacaaattt caattgggaa agttcacgaa actagcaggt
     1801 gaagaagttt caactgttcg gatcaaagaa tgcccgcttc aaatagaaac attggttcaa
     1861 cgaatttcaa ctagacaaga ctttgctatt gtagaacgtg agatcgtagc tatttttgta
     1921 gaagaaggga ttttacatga tccaactcat atcaatgtgg ataaatggca acctcttatc
     1981 tataaattca gagaatatac aactgccaac cagtcgttaa ctttgaattt tagattccaa
     2041 gaattcaaag agtcataatg ataaacatcg ttaggtataa ataaaaaaga ctcgcagcac
     2101 gagtaaaaaa caaatagaag ctatacatta gaaaggtcga ctgtgaccct atagatgcag
     2161 tatatgcagc ttatgagtat ttcttctgtt ctctttgttt ttgggatctg tgctgaagtc
     2221 tttttcgttt tttgccgctc tttctttgaa aaatcgatta tgttatacct tctccattac
     2281 taattcaggc atctgctgag taatgatttt tcgcgcaaaa gaaatatctg ttacccaagg
     2341 ttcgatttgt tcttcagtca agacaagcgg cattcggtca tggattggtg cgactgtgcg
     2401 gttaggttca gtagtcatta tgattgattc tgccattttt tcatcctgat ggctacgata
     2461 aaaaccgcca atatacacta ttttttcctt atttgaaaac ataaatttgt ctttttcttc
     2521 attccactca ttgggaaaac acaacgattt tgcaaaaatg gttgatgaaa ggtctttttt
     2581 tcttctactg tctctgcacg ggcattaatc atcaattgtc cttttttaaa tccggtaaat
     2641 ccccacttca tgatttccgg agtgaggatg ttttcttcct cttttcttaa cgtcacaaca
     2701 agatttgacg gaaaaacttc tccagctgct aattctgctg cctgcttcgg ttgtttcgtc
     2761 tgggcttctc gataataatt ctgaaggagc tctgactcta gatcaaaaaa ataacgacca
     2821 cacatcctaa tccctcccaa tctttttttc tatcatacac tgcaacaaga agttcgcacc
     2881 aacaaaacgc tttcaatttt tgtagaacta aaaaagttgt gacaagtgac aaactctcca
     2941 gtgaatggt
//
LOCUS       DACEUZ010000182         2844 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.182, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000182 DACEUZ010000000
VERSION     DACEUZ010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2844
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.182"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(22..2631)
                     /gene="clpB"
                     /locus_tag="IXG44_002506"
     CDS             complement(22..2631)
                     /gene="clpB"
                     /locus_tag="IXG44_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HAQ1659197.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
BASE COUNT          735 a          623 c          478 g         1008 t
ORIGIN      
        1 ttttatagtc tcaatggaag attagacgat ttcttctagc tcttctgttt tgaaatgaag
       61 ttgtccatca agtaaagtaa tcgttacttt tgatttaggc ataacatgtc ctgcaacgat
      121 ttctttagct agtggtgttt ctacttcctt agtgataaat cgtttcaatg gtcttgcacc
      181 atatgctggt tcataagcat tttcagcaat ccacgttttg gcttcatcac tgattgtcag
      241 caggatttct tgatgttcca gacgttgtgc caattgcgcc accattttgt caacgattcc
      301 tttcacgttg tctaggctca atggtgtaaa taaaatcgtg tcgtcgattc ggttcaagaa
      361 ttctggtttg aagtttccac gtaatagcgt gttgacttgt tcagccactg cttctggaat
      421 cgttccatca gcagtcacgc cttccagcag taactgtgaa ccgatattgc tggtcatgat
      481 cagaaccgta tttttgaaat ctactactcg cccttttgaa tcagtcaagc gtccgtcatc
      541 taggacttgt aacaagatat tgaagacatc gggatgcgct ttttcgattt catccaacaa
      601 gacgattgta taaggatttc gtctgacagc ttccgttaat tgaccacctt cttcgtaacc
      661 tacatagcct ggaggagctc cgactagacg agacacagca tgtttttcca tatattcact
      721 catatcgatc cgtaccatat gatcttctga gtcaaacaag ttctcagcta acgctttcgc
      781 taattcggtt tttccgacac cagttggtcc taggaaaagg aaagaaccta gaggacgatt
      841 tgggtcttgc aatccagccc gtgaacgaat taccgcgtca ctgaccgcat caactgcttc
      901 atcttgacca atcactcgtt tatgaagtgt ttcattcaat ttaattagtt tttctcgttc
      961 accttctaca agtttcgtta cggggatacc cgttaatctg ccaacgactt gagcaatctc
     1021 attttcggta acggactctt gaaccatctt aatctcgctg tcttttgctt tagcttctaa
     1081 ttccttcaat tctttttcta attgaggtat cgtaccatga cgcaatacag cagcgcgttc
     1141 taaatcatag ttattttctg catcttctaa ttcgtgtttt gctttatcga tttcagcacg
     1201 ttttgctgaa acagaattga cttcttcttt ttctgtttcc cactgcatct tcatcgcatt
     1261 tgcttcttca cgaagttcag ctaattcttc ttgcaaattt ttcaagcgtt ttttacttgc
     1321 atcatctgat tcttttttca gtgcagcttc ttcgatttcc aactgcatca aacgtctagt
     1381 aacttgatct aattccgttg gcatagagtt catttctacc cgaatcgttg cactggcttc
     1441 atcgatcaaa tcgattgctt tgtccggtaa gaaccgatca gtaatataac ggtcagataa
     1501 agtagctgct gctactaatg cgttgtcatg gatgttaaca ccatggtgga tctcaaaacg
     1561 ttcttttaat ccacgcagaa tactgatcgt atcttccact gttggttctt tgactaatac
     1621 cttttgaaag cgacgttcca acgctttgtc tttttccata tattgacggt actcatctaa
     1681 agttgtggca ccgatcaaat gtaattcacc gcgagcaagc attggtttca acagatttcc
     1741 ggcatccata cttccttctg tttttcctgc ccctacgata ttgtggattt catcgatgaa
     1801 caagaggata cgtccatcac tttttttgac ttctttcaag actgctttta gtctttcttc
     1861 aaattcacca cggaattttg ctccagcgat caatgcaccc atatctaatg aaaaaatcgt
     1921 tttatctttt aaattttcag gcacatcttt gcgtacgata cgttgagcaa gcccttcaac
     1981 gattgccgtt ttccctacac cgggttctcc tatcagaaca ggattatttt ttgtttttcg
     2041 tgaaagaata cggatgacat cacggatttc ttcatcacga ccgatgattg gatccatttt
     2101 tccgcttttg acttgctgta ctaaatcaac gccgtatttt tctaatgctt tgtattgttc
     2161 ttcttggttt tgtgaggtca ctctttctcc tcctctcatc tcttcgatgt ttttacgcaa
     2221 ttctttttca gataatccgt ttttattcaa ataaacagtt aacggataat tttttaattt
     2281 catcaacgct aaaataacga cttccgttga aagaaactcg tcgccgaagc tttcacgtat
     2341 ttggtctgct tcattcaata atcgaaagaa attctggctg aggttttgtc catattggac
     2401 gtttccacca gaaacaactg gatactcatc cagtagacga tcaatttcat gttcaaaaga
     2461 ttcgacatcc aatccggcat ccgtatagaa atttcgacca aaatgattag attgtaaaaa
     2521 gattttccat acatgggcaa tgtcgatatc ttgatgtttt cgagtgaccg caatctgttg
     2581 agcttcagca atcgcttctt gaagcgtcgt agtcattttc tcaatattca tacaggtata
     2641 ccctcctaat taaaggtaag atcacttatt tctttgatct ttacaaaaac tattatatcg
     2701 ctttggtcaa ttttggtcaa aatatacaca tagttttttg actttttttc tcgacttata
     2761 taaatcttaa aaaaaataaa attgattgta tgtatataaa aaaagagcca aacaaacgta
     2821 aaattatctt atccataacg tttt
//
LOCUS       DACEUZ010000183         2842 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.183, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000183 DACEUZ010000000
VERSION     DACEUZ010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2842
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.183"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            95..370
                     /locus_tag="IXG44_002507"
     CDS             95..370
                     /locus_tag="IXG44_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HAQ1659198.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            808..1758
                     /gene="yidC"
                     /locus_tag="IXG44_002508"
     CDS             808..1758
                     /gene="yidC"
                     /locus_tag="IXG44_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAQ1659199.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     IDVTESAKPADSKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(1937..2137)
                     /locus_tag="IXG44_002509"
     CDS             complement(1937..2137)
                     /locus_tag="IXG44_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1659200.1"
                     /translation="MGGISALSFMENGVLCGRATWAKGVAPFVTEGEEKASSWMLEEG
                     KQNIKELSTLLEETATPVYVDR"
BASE COUNT          892 a          564 c          523 g          863 t
ORIGIN      
        1 ccatgattat atcatggaat aggtgcttga gcttgtttca tcctaaaaaa atcggtatca
       61 ttaatttata aaataggaaa gaaaggtgac gaacatgcgc aaagttaaaa tgaacgtcca
      121 aggacgcgtc caaggcgtag gatttcgcta catgaccaag atggtcgctg atcaactagg
      181 cgtgactgga acggtaaaaa acgaagatga cggctcagtc acgatcgaag caatcggcaa
      241 tgacgacatc atccagaaat ttattgaaga agtcaagaaa tctccgagcc caagtggacg
      301 agtgcagtat gtggatgtcc aagaagaccc gatgattgag gaacgaaaga aatttgatgt
      361 ggtgggataa ggttgtgaaa aagctggtct gcatcaagca ttaaggacag gttgtgaaaa
      421 atagctctcc atatttttca acaggctgga cttaatgccg tagatgcaag cttttgaaca
      481 ccgtcgataa ggttattctt gaggcgcttt gcctcaaaaa ttaaggacag cagtgaaaaa
      541 gctgatctat cacttgcagt gagttcattt gactccgctt tttcgggcag aaaaaaagtt
      601 tttagtttat taaagtaaga aagaaacact tggaatcatt ctactttttc ctagtatttc
      661 tttcttgatt ttttgtcttt ttttcaaaat ttttcgctac gtaaaagaac gaatatttat
      721 atgaaatttc tatgaagttg attgaattcc atgcgctttt ttagtatagt taagtttgtg
      781 taaaaattat agaagaggaa gtattctatg aataaatgga aaaactggtt attgggatcc
      841 ggacttgtca gtatcctgct ctttttatct ggctgtgtgc ggatggattc aaacggaaac
      901 cctgacactt cgggaatcgt ttatcgagta ctcgtccatc ctatgggcca agcgattaca
      961 tatctagttg aaaatttcaa ttggtcttat ggctgggcag ttattgcaat gactgtgatc
     1021 gtccgtatca tcatcttgcc actaggtatt agtcaatcga aaaaaacaat gatccaatct
     1081 gaaaaaatgc aagcgttaaa accacaagtt gaagcagccc aacaaaaatt gaaagctgca
     1141 acaacacgtg aagaacaaat ggcagcacaa gcagaaatgc agcaagtata ccgcgaaaac
     1201 ggattgagta tgaccggcgg aatcggctgt ctgcctctat tgatccaaat gccgatcttc
     1261 tctgccttgt actttacggc acgttataca gaaggtatcc gggaatcatc tttctacggc
     1321 atcgatttag gctcaccaag tatggtactt gttgccattg caggggtcgc ttatctgctt
     1381 caaggataca tctcaacgat cggtattcct gaagaacaga aaaagacgat gcgtacgatg
     1441 ctgatcgtct ctcctgctat gatcgtcttc atgtcgatca gtgcgccagc cggtgttaca
     1501 ctttactggg tagtcggtgg tgtgttcagc tgtctgcaaa cattcatcac aaacgttatg
     1561 atgaaaccac gtttgaaagc acaagtggca gaagagttga aacaaaaccc accaaaacaa
     1621 gtcgtcactc caagaattga tgtgacagaa tcagctaaac cagctgacag caaacaagca
     1681 accaaacaaa tcaagcaacc caaaaacagt acaaacaata aaggccgcaa cgcaggaaag
     1741 cagcagaagc ggaagtagat agtaaataag agtgtaataa aaattcatta acagttttag
     1801 caattaaaaa tatcgaccaa gatttttgaa aaagaaatct ggtcgatatt tttatttaag
     1861 tgaatgttta atttgaatca tcgattgatc gctatctaat tgcagcagca tctttttatg
     1921 aagaaacaac tttttcttat ctatcaacgt agacaggtgt tgctgtttct tctaatagtg
     1981 tacttaattc cttaatattt tgttttcctt cttctaacat ccaactactc gctttttctt
     2041 cgccttcggt aacaaaaggc gccactccct tcgcccatgt tgctcgccca cataagacac
     2101 cattctccat aaaggacaac gcggagatac cacccatcta aataacatcc ataaaccttt
     2161 ctagtagaag aatagtgata gtatccatcc ctttttctac tgggaataca attacacgat
     2221 tcaaagcttg atacacaaca tttcccttct tataaaccgc tattgtaacc gaattgtttt
     2281 gaatcacctt tttgggatag aaactggctc ctttccgcaa cttcttaaat gttggtttct
     2341 tttatacttt ctttgtagca actacaaatt tcttcaaatc tctaaatgtt tgcataacaa
     2401 ccgcctttct gacttttact caggatggta tttaaaaatc agcaaaataa ggcttctctc
     2461 catctttttt atttcctagc tgctttaaag acactcttgc tgttttttaa tctctatcaa
     2521 gggggctctg aatctatttt gatgaactta tcttattaat ttctcaaatg agctgtcaga
     2581 cacaactgta ttttttcaat ctcataccgg acaactatgc cagcttaaaa aaatttcctc
     2641 tggcaaaact tctttattga aaaatgacaa tgaaaaaaga ccattctttt tgttgaatgc
     2701 tcattgtgta aacatatttc ctgcgactgc tcttcgaacc attctcagtg caatccctaa
     2761 tttatctatt aaatcttctt ttagttcctc atctttcata ttcaaaattt cctttgtatg
     2821 ttacacaagt ttgttctcta aa
//
LOCUS       DACEUZ010000184         2778 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.184, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000184 DACEUZ010000000
VERSION     DACEUZ010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2778
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.184"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            <1..197
                     /locus_tag="IXG44_002510"
     CDS             <1..197
                     /locus_tag="IXG44_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1659201.1"
                     /translation="WFLSNAQSLVLLAIVVIGLYLGFKREFSKLIGFLIIAIIAVGLV
                     FNAAGVKDILLELFNRIIGA"
     gene            314..811
                     /locus_tag="IXG44_002511"
     CDS             314..811
                     /locus_tag="IXG44_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAQ1659202.1"
                     /translation="MDDMQVYIANLGKYNEGELVGAWFTFPIDFEEVKEKIGLNDEYE
                     EYAIHDYELPFTVDEYTSISELNRLWEMVSELPEELQSELSALLTHFSSIEELSEHQE
                     DIIIHSDCDDMSDVARYYIEETGALGEVPASLQNYIDYESYGRDLDLSGTFISTNHGI
                     FEIVY"
     gene            898..1290
                     /locus_tag="IXG44_002512"
     CDS             898..1290
                     /locus_tag="IXG44_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ1659203.1"
                     /translation="MKKIRSYTSIWSVEKVLYSINDFRLPFPITFTQMTWFVVSLFAV
                     MILGNLPPLSMIEGAFLKYFGIPVAFTWFMSTKTFDGKKPYGFLKSVIAYALRRKLTY
                     AGKKVTLGRNQPQEAITAVRSEFYGISN"
     gene            1274..>2778
                     /locus_tag="IXG44_002513"
     CDS             1274..>2778
                     /locus_tag="IXG44_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HAQ1659204.1"
                     /translation="MAYPIKYIENNLVWNKDGECYAYYELVPYNYSFLSPEQKIQVHD
                     SFRQLIAQNRDGKIHALQISTESSILSAQERSKNEVTGKLKTVAYDKIDQQTDALISM
                     IGENQVDYRFFIGFKLLLNDQEFSMKSLTVEAKNAFTDFVYDVNHKLMGDFVSMSNDE
                     ILRFQKMEKLLENKISRRFKIRRLDKDDFGYLIEHLYGQTGTAYEEYEYPLSKKKLEH
                     ETLIKTYDLIKPTRCLVEEKQRYLKIQQEDETAYVAYFTINSIVGELDFPSSEIFYYQ
                     QQQFTFPIDTSMNVEIVANRKALSTVRNKKKELKDLDNHAWQSDNETSSNVAEALESV
                     NELETNLDQSKESMYKLSYVVRVSANDLDELKRRCNEVKDFYDDLSVKLVRPFGDMLG
                     LHEEFLPASKRYMNDYIQYVTSDFLAGLGFGATQMLGENEGIYVGYSLDTGRNVYLKP
                     ALASQGVKGSVTNALASAFVGSLGGGKSFANNLIVYYAVLYGAQAVIVDPKA"
BASE COUNT          839 a          518 c          596 g          825 t
ORIGIN      
        1 actggtttct atcaaacgct caatcactgg tgcttttagc aatcgttgtg attggcttgt
       61 atcttggctt caagcgtgag tttagcaaac tgattggctt tttaattatt gcgattattg
      121 cggttggctt agtcttcaac gctgctggag taaaagacat tttactagag ctattcaatc
      181 gcattattgg tgcttaaata aaaccgttct tttgtggaat ataagtggtt ttcttatgtt
      241 ccgcaaagga atggtacacc aaacgaagtg cggtagggat ttttgaatct ctacaaagaa
      301 aggacgtgaa tatatggacg atatgcaagt ctatattgcg aatttaggca aatacaatga
      361 gggcgaattg gtcggtgcgt ggtttacctt tcccattgac tttgaggaag tcaaagagaa
      421 aatcggcttg aatgatgaat atgaggaata cgccattcat gattacgagt taccctttac
      481 ggttgacgaa tacacttcta ttagcgaact caatcgccta tgggaaatgg tatcggagtt
      541 gccagaagaa ctacaatcgg agctatctgc tctgctcact catttttcaa gtattgaaga
      601 actaagcgaa catcaagaag atattatcat tcattccgat tgtgatgata tgtctgacgt
      661 ggcacgctac tacattgaag aaacgggtgc tttaggcgaa gtgccagcca gtcttcaaaa
      721 ctatattgat tatgaatcct atggtcggga tttagacctt tcgggaacgt ttatctcaac
      781 caatcacggg atttttgaaa tcgtctatta aatctgtcgg tacattctta ctggcagatt
      841 ttcttgttta cggggtagtc aaaagactat ccccattttt atgaaaggat tgattacatg
      901 aagaaaatac gaagctatac cagtatctgg tctgttgaga aagtgttgta ctccatcaat
      961 gactttagac ttccgtttcc cataaccttt acgcaaatga catggtttgt cgtgtcactc
     1021 tttgcagtta tgatacttgg caacttgccc cctctttcta tgatagaggg agcatttctc
     1081 aaatactttg ggattcctgt ggctttcaca tggtttatgt ctacaaaaac ctttgatggt
     1141 aaaaagcctt atggattttt gaagtctgtc attgcttatg cactgcgacg aaagctgacc
     1201 tatgcaggaa aaaaagtaac gcttggcaga aaccagccac aagaagccat tacagcagtt
     1261 aggagtgaat tttatggcat atccaattaa atatattgaa aacaatctgg tctggaataa
     1321 agatggggaa tgttacgctt actatgagct tgttccctac aattactcat ttctaagtcc
     1381 ggaacagaaa atacaagtac atgattcttt cagacagctt atcgcacaaa atcgtgatgg
     1441 caagattcat gctttacaaa tcagtacaga atccagcata ctttctgcac aagaacgttc
     1501 caaaaatgaa gtcactggaa agctcaaaac ggttgcctat gacaaaatcg accaacagac
     1561 agacgcttta atatccatga ttggcgaaaa tcaagtggac taccgtttct ttatcggttt
     1621 taagttgctt ctcaacgatc aggagttttc tatgaaaagt cttaccgttg aagcaaaaaa
     1681 tgcttttact gattttgtct atgatgtgaa ccataagctg atgggcgatt ttgttagtat
     1741 gagtaatgat gaaatcctgc gttttcagaa gatggaaaag cttttagaaa ataaaatctc
     1801 ccgtcgtttc aaaatccgaa gattagataa ggatgacttc ggctatctga ttgaacacct
     1861 ttacggacag acaggcactg cctatgaaga gtatgagtat cctctatcaa agaaaaagct
     1921 ggaacatgaa acactgatta aaacctatga cctcattaag cctactcgct gtctggtgga
     1981 agaaaaacag cgatatttga aaatccagca ggaagacgaa accgcctatg tagcttactt
     2041 taccatcaac agtattgtcg gcgaactgga cttcccgtcc tctgaaatct tctactacca
     2101 gcaacagcaa tttacctttc cgattgatac gtcaatgaat gtggaaattg tagcgaatcg
     2161 taaagcccta tctactgtcc gcaataaaaa gaaagaactg aaagacttgg ataaccacgc
     2221 ttggcaaagt gataatgaaa ccagctctaa tgtggcggaa gctctggaaa gtgtgaacga
     2281 gctggaaacc aatttagacc aaagcaagga atctatgtat aagctgtcct atgttgtaag
     2341 ggtatcagca aatgaccttg acgaacttaa acgtcgttgt aatgaagtga aagatttcta
     2401 tgatgatttg agcgtgaaac tggtacgacc ttttggggat atgctgggct tacatgaaga
     2461 atttttacct gccagcaaaa gatatatgaa tgactatatt caatacgtga cctctgattt
     2521 cctcgctggt ttaggttttg gtgctactca aatgctgggt gaaaatgagg ggatttatgt
     2581 tggctacagc ttagatactg gacgcaatgt ctatctgaaa cctgctcttg ccagtcaagg
     2641 ggttaagggt tcagtaacca atgcgttagc gtctgccttt gtcggttcgc tgggtggtgg
     2701 taaatccttt gcgaataacc ttatcgtcta ttatgcagtg ctttatgggg cacaagcagt
     2761 gattgtagac ccaaaagc
//
LOCUS       DACEUZ010000185         2772 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.185, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000185 DACEUZ010000000
VERSION     DACEUZ010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2772
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.185"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            178..774
                     /locus_tag="IXG44_002514"
     CDS             178..774
                     /locus_tag="IXG44_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAQ1659205.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
     gene            898..2697
                     /locus_tag="IXG44_002515"
     CDS             898..2697
                     /locus_tag="IXG44_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAQ1659206.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
BASE COUNT          790 a          479 c          523 g          980 t
ORIGIN      
        1 ttaaaaaaaa caagttatta aataagtctt tagttggatg tttttttgag atcaatcttt
       61 tatacttatg atgaacttac aaaattgttt gttttctgtc atatcaaaaa atggcgcagt
      121 tagttgcttt tctagtaaag tatagttaac aaagaacctc taaggagttg atgaataatg
      181 aatattgctg cctctcgaaa actgataaat ttcatatcca ttatcggatt aggcctatca
      241 atcgcgctga cgatttattt tattaattta ggtgttttca aagatttgaa tgcgttaaga
      301 ggattagtag gtgattccat cattttagga ccgattattt ttgtcttcat ccaaatcctg
      361 caagtagtta ttccaatcat tcctggaggg atcagtactg cagctggtgt cctgatattt
      421 ggcccttatg ctggatttat ctataactat attggaatct gcattggttc gattatcatt
      481 tttctgctag ggcgtcgtta tggaaagcca ttcatacttt ccatgattag cgataagaca
      541 tataacaaat atattggctg gctggataat caaaaccgtt ttgagaagct gtttgctcta
      601 gctatttttc ttccagttgc tcctgacgat gcgttatgtt tgatggctgg attgacaaac
      661 atgtcagtga aacgatatac actaatcatt ttgattgcta aaccactgtc gattttctta
      721 tatagtatgg ccttgattta tggaggtcaa tatctaagtg gtcttcttgg ataatattta
      781 ttttgtaaaa ttagtcattg attgacaatg ttaaatctgg gaaagggtct gcaaaacaca
      841 ctgcaccctt tcctttttca ttagatttgc gctacaatgt gaagagcagg tgataggatg
      901 aattatttga aacgtagctt aggtaatgct tgggactttt taaaaggaac acaagcttat
      961 ttccgtgatg tcttgctgat gcatggcttt attttattta tttgtttacc cttattgagc
     1021 agtacgaccc gttttatttt acaccgaggg gcaattgact atttgtctta tgacaatatt
     1081 ccttcaattt tttctcaaca tcctggggtt ctactctcgt taatcgtagt tttgatgacg
     1141 attttactgt tagtttattt cgaatttact tttcttttga tgagtgtttt ttttattaaa
     1201 aagaaagaac caatctcttt gaagcaactc ttacacttga cgatacttca gctgaagaaa
     1261 gtgagaccca ttactttttt gtttttcctt gcttattttt tattgattct accgatcagc
     1321 ggattaagtt ttaattctga tctactgtca aaaataaaaa tccccgcttt cattatggat
     1381 tttatttttg ctaatcgttg gatcatcgtc agttcctttt tactcgttta tattttcctt
     1441 ggctatattg ggattcgact gatttttgct ttaccggaaa tgattctacg ggatcgtcct
     1501 tttcgagcag ccattcggga aagctggtca ttgaccaaat cgcgtctttt ggccatcacg
     1561 ggccaattta ttgtgattgg cggtacgatt ttactccttt cttctttagg atatatcgtt
     1621 gttattttgg cacaaagtat ggttgaacaa tttttcccag actatgcatt gatcagcgct
     1681 gtgtttgcta tgacgctttt acaagggatt ttattattca atatcgtgat gtctaccgta
     1741 gggatttttt atatcattgt tgatttcatg gatgacgaag gcttcttgcc tgagatccct
     1801 aagtggttta tcccacaagc ccccaacttg cgtttctctg cgctgaaaaa cactggtttg
     1861 actctttttg cagttttttt cggtattggc gtctgtcttt acaatatgga ttatctgact
     1921 tcagctgttc agacaaaacc agtgactgtc tctcacagag gtgtgagtac ccaaaatggg
     1981 gcgcaaaata ctttagaagc acttgaaaaa acaagtagag actatcatcc tgattatgta
     2041 gagatggatg ttcaggaaac aaaagatggt catttcgtag tgatgcatga tgcaaatctt
     2101 cgtcacttaa caggggtaaa tggaacacca caggatctta cattgaaaga attgacaaat
     2161 cttacagtta ctgaaaacgg agtgcaagcc cctgtttgtt cttttgattc ttatttggaa
     2221 aaagcacata agctaaacca aaaattactt attgagatca agacctctaa aaaagattcg
     2281 gatgaccttg tcgaacgatt tgtccataaa taccgcaaag ttatcttaga gaaccatcat
     2341 attttgcaaa gtttgacgta ccaaaccgtt tcagatttaa aagaagcaga accaaacttt
     2401 tatgtgggct acatcttacc tttcaatatt gttgggcccc ctgtgacgcc tgctgatttt
     2461 ctgacaatgg aatatagtac gatcaaccgt aacttcatta attctgccca ccaagacgga
     2521 aaagaagtat atgtatggac aatcaacgat tcagatgcta tgagtcgcat gatgttttat
     2581 ggtgtggatg gtatcataac cgatgagatg ggactactaa atgaaagtgt gaaacaaaca
     2641 gatgaagaaa tcacttattc agataaactg ctaaattttg ttttgggagt cggctaatca
     2701 aaaatcgtta atgaaaaaag tctttataca acaaaaaacg cataacctgc caccgcaggt
     2761 atatgcgttt tt
//
LOCUS       DACEUZ010000186         2769 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.186, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000186 DACEUZ010000000
VERSION     DACEUZ010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2769
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.186"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(4..324)
                     /locus_tag="IXG44_002516"
     CDS             complement(4..324)
                     /locus_tag="IXG44_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1659207.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            complement(432..719)
                     /locus_tag="IXG44_002517"
     CDS             complement(432..719)
                     /locus_tag="IXG44_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1659208.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            846..1490
                     /locus_tag="IXG44_002518"
     CDS             846..1490
                     /locus_tag="IXG44_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HAQ1659209.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            complement(1550..2257)
                     /locus_tag="IXG44_002519"
     CDS             complement(1550..2257)
                     /locus_tag="IXG44_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1659210.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
BASE COUNT          832 a          579 c          469 g          889 t
ORIGIN      
        1 tatttattca cctatgagcg tgattgcttg atctaacact ttttcgccat tcatcatacc
       61 ataatcagcc atattgatga cgtctaaagg aatgcctttt ggttccagac gtttttcaaa
      121 atcagctttc ataaagcgaa cttgaggtcc taataacagt acatctacat ctttatttgc
      181 aagattgtta tctgcgtcag aagcggacac agcgaaaata tccgagtcga ttccttttgc
      241 ttcagcagct ttttgcattt ttgttactag taaacttgtg ctcataccag ccgaacatac
      301 aagcatgatt gtttttttag ccatactttt ctctcctttt taaaacaaac tacgcaaacg
      361 ttttcttcgc gtctgttact ctattatata aaaagaaaga aatttgtcaa cgttttctaa
      421 acgcggttta tttaatcaaa tagccaacta gatttcacta atttattgaa cacgacatat
      481 tctttgtcca aattgatgac aactacttca tagcctgcat ttatccagcc ataggcacct
      541 ttttcagggt gtttataatc atttgcccac caatctgtac tttcccttgc tgttttggga
      601 agtccgttac tgggaaacag cagttcgtca aattgggtaa aggtcaacgt tacttgagag
      661 ccgccattgt tcttcaaata atgcgtaatt gcatcgtact ttttcattcg ttcctgcatc
      721 cccagatcca cctacctttg tctttattat cgcatactac agcgattgcg caaccgttac
      781 agtgttgaaa caacctagaa ctttatttat actgaataaa gaaaatgaaa aaaagaggtg
      841 acaagatgat cgagcgctat cgtacaataa aagaaaatgg acaaagtgag attgagatca
      901 aaaaatcacg ctttatctgt tctcttagac gaatagaatc agaggaccaa gcaaaaagtt
      961 tcattcaaga aataaaaaaa gaacattgga aagcgaacca taactgcagc gccttcatca
     1021 ttggagaaaa aaatgaaatc cagcgaagca gcgatgatgg agaaccaagt ggaacggctg
     1081 gcgtaccgat gttggaagtc ctcaaaaaaa atcaactgat caatgtagct gcagtagtca
     1141 ctcgatattt tggaggaacg aaactcggca caggcggatt aatacgagca tacactcatg
     1201 ctgtctctca agcattgtct gacattggaa tcgtagaagg taccttgcaa caggagatct
     1261 ctgtgatgat cacgtatccg caactaggaa aattacagaa ttatcttgaa ctccaacatt
     1321 tttttataaa agaaatccaa tatactgatc aagttagcgt catctgtgct gtagatgaaa
     1381 aaagtgctga ccactttaaa gaatccatca ttgagctgct gaacggacaa gtccaatttg
     1441 aagagggtcc tgtttcttat cacgagcaac tggtaactga tcatatataa aaacaggtga
     1501 ttctgattct cttttatttc taagtaagga atcagggatc acctgttttt tatttttcca
     1561 aataaaactc aaaacgattt cctacgtatt gcgtacgcac atactcaaac ggcgtaccgt
     1621 cttcaaaata agaaatctgg cgcaaacgaa gaactgcatc tcctcgtttt acctctaaat
     1681 agtcagcaat tttctcagaa gccaacacag cagtaatcgt ttgattcgct ggaccgatct
     1741 ttttcccgcc tttttcttct agcgtgcgat acaacgattc agtaatctct gctttgctgt
     1801 agccatcgat cagcttttgt ggcacactgg caacttcaaa acaaataggg acatcatctg
     1861 cgaaacgaat acgttccatt cgcaagatcg tgtcatcttt actcaagttt agtttttcca
     1921 tttcacttga gcttggcgaa gtcaagaaat aagagattgt ccgactagac ggtactcgat
     1981 tttgcgattc cataatttct gtaaaacttg ttgcaccaga cattttttct tgtacttttt
     2041 ctcgcgcaac atatgtaccc gaaccgatct ttctttctaa gattccttca tctgccaagt
     2101 tttgaattgc ttgtctcaac gtcatacggc tgacattgaa tttcactgct aattctcttt
     2161 ctgaaggaag acggtctccg actttccata taccttgttc gatttcattt tttaattggt
     2221 catgaatctg tatatataca ggtaattgat tcgacataac gtacacccgt cctttcgttt
     2281 cttctattat aactggtata tacctgattc acaactgaaa aagataaagg tttctatcca
     2341 tcaacagagg atttagacac acatcgttct tcctactcct attgtcagaa acaatttttt
     2401 agatatgttc aacctagaaa ataacaaaag accctaaaat cgatgaaaat gccgacttat
     2461 ggatcttttg ttattattag cggtttttag ccatccattt ttttgtatga ttcgttgggc
     2521 gttttgattt tacctggacc gcttttccgc cttttttcgg aatattcact tcaatattgt
     2581 atttttctga tttgaccaaa tcatataact tttcagctgc ttcgtctact ttttctttcc
     2641 atttcatgtg gatccctcca attcttgtag ttaacttaac tctatcttaa atcagcagaa
     2701 tcttcaagga acaaggtagc aaagcaaaaa aaagagctct cgaactaacg agagcttacg
     2761 attgtggta
//
LOCUS       DACEUZ010000187         2650 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.187, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000187 DACEUZ010000000
VERSION     DACEUZ010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2650
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.187"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            36..197
                     /locus_tag="IXG44_002520"
     CDS             36..197
                     /locus_tag="IXG44_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002392227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1659211.1"
                     /translation="MAIVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGVKDVLL
                     SLFNRIVGA"
     gene            210..713
                     /locus_tag="IXG44_002521"
     CDS             210..713
                     /locus_tag="IXG44_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAQ1659212.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            773..1162
                     /locus_tag="IXG44_002522"
     CDS             773..1162
                     /locus_tag="IXG44_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ1659213.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            1149..>2650
                     /locus_tag="IXG44_002523"
     CDS             1149..>2650
                     /locus_tag="IXG44_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HAQ1659214.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKG"
BASE COUNT          788 a          439 c          610 g          813 t
ORIGIN      
        1 aatggttttt aaccaatgcc caatccttag tcttactggc aattgtggtc attggtttgt
       61 ttttaggatt taagcgggag ttttccaaac tgattggctt tttggtgatt gctttgattg
      121 cggtcggatt ggtctttaat gcttctgggg tgaaggatgt cttactcagt ttgtttaacc
      181 gaattgtcgg tgcgtaaggg ggaagcacga tggaacagat gcgtgtttat attgcgaacc
      241 tcgggaaata taacgaaggt gaattggtgg gggcgtggtt taccccacct gtcgattttg
      301 atgaagtgaa agaacgaatt gggttaaatg acgaatacga ggaatatgca attcatgatt
      361 atgaacttcc ctttgagatt gatgagtata cgccaattga ggaaattaat cggttgtgtg
      421 gcttagcgga ggaacttgaa ggaacgccag ttggtgaagt tgcttctgag attcaacatg
      481 ctttcttcaa ttcttttgag gagatggtag agcatgtgga tgatattatc tattatccag
      541 attgcgatga tatgagtgat gtagcatatt tcttgattgt agaagctggt gattttggag
      601 aagtaccaga acacttgaag acgtatattg attttgatgc gtgtggtaga gacttggaga
      661 ttggaggcaa ttatttggta acgagtcgag gtgtttttga gtatccatat taacaaaatt
      721 attgaagggt agttgatttc taaactatcc ttctttttaa aggaatgatt tgatgaagaa
      781 aataaaaagc tacaccagta tttgggcagt tgaaaaggtc atttatgcaa ttaatgactt
      841 tcaactgcct tttcctgtga cttttaacca gatggcttgg tttgttttat ccttgctgtt
      901 tgtcatcgta tttgcccatg tgcctccact atctatgata gagggggcct ttttaaaata
      961 cttaggtata ccggtggcag ttacttggtt catgtcgcaa aaaacgtttg acggtaagaa
     1021 accattggga tttttacgat cttttattag ctatcactta cgatttaagg tcactttcgc
     1081 aggaaagaaa gtcaaagaac agaagaaacg atgggacgaa cccattactc tggtaaggag
     1141 tgtgaactat gtacccgatt aagtatattg aaaataatct tgtctttaat caagagggag
     1201 aatgttttgc ctactatgag ttaatacctt acaattactc ctttttgtca cctgaacaga
     1261 aatatcaagt ccacgataac tttcgtcaat tgattgcgca aaatcgtgaa ggcaagattc
     1321 atgccttaca aattgccaca gaaagtagta ttcgtgccac gcaagaacga tcaaaaaagg
     1381 aaattaccgg gcgattaaca gaagtagcga aacaacgaat tgatttacag acagaggcat
     1441 tagtatccat gattggcgat agtcagattg attatcgctt ttttattggc ttcaaactga
     1501 ttgcgacaga tgaagaggtc aatctaaaga atctcaagaa atctttcttc tccggctttc
     1561 aagaatttgt ctatggagtg aatcatcatt tgatgggcga ttttgtttcc ttatccaatg
     1621 aagaaattcg ccgatatacc aagcttgaaa aactaatgga aagtaaattg gctcgaagat
     1681 ttaaagtcag acgagtcaca ccgagtgatc tgatgtattt gattgaacat atctatggcg
     1741 aaaagggtac accatttgaa gaatatgaat ttcagctacc aaagaaaaag ctgaagtcgg
     1801 aaactttggt gaaacgttac gacttattac gtcccagtcg ttgtttgatt gaagaaaagc
     1861 cccggtactt atgcatggaa catgagaatc atgaatcata tgtagcgtac ttaacaatta
     1921 atacaattgt gggggaaatg gagtttccct cctcagaact tttctattac cagcaacaac
     1981 aatttacatt cccaattgac actagtatga atgtggagat tgtgacgaac aaaaaggcac
     2041 ttgctaccgt tcgcaataag aaaaaggaat taaaagactt ggacaaccat gcctatcaat
     2101 ctgacaatga aacaaattcc aatgtcttag atgcattgga ttccgtagat gaattggaaa
     2161 ctaccttgga tcagtccaaa gaatccatgt acaaactgag ctatgtggtt cgagtaagtg
     2221 ccgaatcagt agatgaacta aagcgccggt gtgatgaagt gctagatttt tatgatgata
     2281 ccaatgtaaa actagtccgg ccttttggcg atatgatggg gctacatgaa gaattcttac
     2341 cttctagtaa gcgatacatg aatgattata tccagtatgt tacgtctgat tttctcgccg
     2401 gacttggttt tggcgccact cagatgttag gggagttgga aggcatttac tttggttata
     2461 acgtagatac ggggcgcaat gtttacctga aaccggcatt ggcctcacaa ggggtcaagg
     2521 gttcagttac caatgcgcta gcagcggcat tcctcggttc actcggtggc ggaaagtcct
     2581 ttagtaacaa tctcttagtt tattacgccg tcctttttgg tggacaagct gtgattgtcg
     2641 atccaaaagg
//
LOCUS       DACEUZ010000188         2625 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.188, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000188 DACEUZ010000000
VERSION     DACEUZ010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2625
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.188"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            724..2550
                     /gene="ltrA"
                     /locus_tag="IXG44_002524"
     CDS             724..2550
                     /gene="ltrA"
                     /locus_tag="IXG44_002524"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAQ1659215.1"
                     /translation="MLKKNKLRYNEYYDMQHIYDELYAQSKNGNNFYKLLEIIGSEQN
                     ICLAYRNLKSNSGSKTAGTDGMTIDDIKQLSNAEIVATVRESLSNYRPKSVRRVFIPK
                     AGSDKMRPLGIPCIWDRLVQQCILQVLEPICEPKFHNHSYGFRANRSAHHAVSRVTTL
                     INLSKYHYCVDVDIKGFFDNVNHGKLLKQIWTLGIRDKRLICIISKMLKAEIDGEGVP
                     EKGTPQGGLLSPLLSLIVLNELDWWVSSQWETFQPKNRSKNGWLQYAKKYTKLKSGFI
                     VRYADDFKIMCSTYGEAQRFYHSTVDFLNKRLKLEISPEKSKVVNLKKNSSDFLGFKI
                     KVIPKGRTKHGYVAKTDMNQKALKKAKTNLKLKVKDIARHTTGFNISRYNLTVIGMQN
                     YYCIATNVYNNLTEVSYALLPTIRIRLRNIAKSVPFESTSSEFQSRTKGIRPKTKIVM
                     IADNPLLPIQGVQHKNPMNFSQDICNFTKQGRNKVHEDVVVVTKEEIRALLENENPAD
                     SVEFNDNRISAYIAQQGNCYVMNRRGTPSTLICIHKSDKGDNLDRYSNLAFVEIPIAK
                     AILTESADEAKSLLKGYVLNSQQKKKLNRIRTNYGYQTITGK"
BASE COUNT          943 a          458 c          581 g          643 t
ORIGIN      
        1 aaaagagtag gcacagcact tcattaaatc aatatttaat gtgtgttgga gaactaaaca
       61 cacgaatagt cgaatgacgg ggtaacgccc tgaaaggcta ctttaatact ctgactggca
      121 gaaacttgag taaacaaggc tctgtcagaa gctcggtgaa gtcggcagac agacaccgta
      181 acagataatg ctgacgaaag attcccagag gtgggcgttg tgtcctatat gccggaagtc
      241 tataaaatat ctatggttag aatgtagatg agaggtctac tgacgaatcc ttgaatgtac
      301 aggtctatat cggggctgga tagaaatgtc cagtgacaca aacagtgtcg ctactgtggg
      361 tgagtaagac tccgcattta tgaaaatcca tataacgtta aaggcgttat caaggtagca
      421 ggcttataga ggaaacctaa ggatatatgt acagatagtg tgtttggaac gttgaaagct
      481 gacaacatgg agattttact gtctgtgaag tgttcacatc agaaagttat tgtgagatta
      541 gcaatgatat aaggtgtgtt aatgtcagtg agagtggtgg cacagtacct atgaaaccgt
      601 gataacaagc ggtggaggga tagccactag tcatatgaag aatagaatta ctaagaaaaa
      661 tggtatgggt tcgagtaaga ctaagagatg tacttgctcc tgcaaacaag gagggtacag
      721 accatgctaa agaaaaacaa gctccgctat aacgagtatt atgatatgca acatatttat
      781 gacgagttat acgcacagag caagaacggt aataactttt ataagttact tgaaattatt
      841 ggttcggaac aaaatatttg tttagcttac cgtaatctga aatcaaacag tggcagtaaa
      901 acggctggaa cggatggaat gacgatagac gatataaaac aactcagcaa tgctgagatt
      961 gttgctaccg ttcgggaaag ccttagcaac taccgcccca aatcagtcag acgtgttttc
     1021 attccaaaag ctgggagcga taaaatgcgt ccattgggga ttccgtgtat ctgggacagg
     1081 ctggtacaac aatgtatcct gcaagtccta gagccgatat gcgaaccaaa gttccataat
     1141 cattcctatg gattccgtgc gaatcgaagt gcccaccatg cagtcagcag agtaacgaca
     1201 ctgataaatt tatcaaaata tcattactgt gttgacgtag acattaaagg atttttcgat
     1261 aatgtcaatc atggaaaact attaaaacaa atctggacac ttggtattcg ggataaaaga
     1321 ttgatttgca ttatcagcaa aatgctgaaa gccgaaattg acggcgaggg tgtcccagaa
     1381 aaaggaacgc cacaaggcgg tctgttaagt ccactgctct ccctcatagt ccttaatgaa
     1441 ttagactggt gggtaagcag tcagtgggaa acattccagc cgaagaaccg cagtaaaaat
     1501 gggtggcttc aatacgcaaa gaaatatacc aaactgaaaa gcggttttat tgtacgttac
     1561 gctgatgatt ttaaaatcat gtgttcaacc tatggagaag cacaaagatt ttaccacagt
     1621 accgtagatt ttcttaataa aaggctgaaa ttagaaatca gtccagagaa atcaaaggta
     1681 gtaaatctga agaaaaattc atcagatttt ctggggttca aaatcaaagt cattccaaaa
     1741 ggaagaacga aacacggtta tgttgccaaa acagatatga accaaaaagc attaaagaaa
     1801 gcgaaaacaa atctaaagtt aaaggtaaaa gatatagcca gacatacgac tggcttcaat
     1861 atcagcagat ataatctgac agttatcggt atgcagaatt actactgcat agcaactaac
     1921 gtatataaca acctgacaga agtaagttac gctctcttac cgaccatacg aatccgttta
     1981 aggaacattg caaaatcagt tccatttgaa agtacaagtt cagaatttca gagcaggaca
     2041 aaaggaatca gaccaaaaac aaaaatagtg atgattgcgg ataatccgct attgccgatt
     2101 caaggagtac aacacaaaaa tcctatgaat ttctcgcaag atatttgtaa ttttacaaag
     2161 caagggagaa acaaggtgca tgaggacgtt gtagttgtaa caaaagaaga aatccgagcg
     2221 ttgttggaaa atgaaaaccc tgccgatagc gtagagttta acgacaatcg tatttcggca
     2281 tatatcgcac aacaaggcaa ctgctatgta atgaatcgtc gaggaactcc gtccactctg
     2341 atatgtattc acaaatcaga caaaggagat aaccttgaca gatattctaa tctggcattt
     2401 gtggaaattc ccattgcaaa ggcaatccta acagaatcag cagacgaagc aaaatcactg
     2461 ttaaaaggtt atgttcttaa tagtcagcag aagaaaaaac tgaacagaat acggacgaat
     2521 tacggatacc agacaataac tgggaaataa cttataaatc tatttggata tgatggaacg
     2581 ccgtatgaag cgaaagtttc atgtacggtg tgaagcgggg gaaaa
//
LOCUS       DACEUZ010000189         2535 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.189, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000189 DACEUZ010000000
VERSION     DACEUZ010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2535
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.189"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(51..1517)
                     /locus_tag="IXG44_002525"
     CDS             complement(51..1517)
                     /locus_tag="IXG44_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HAQ1659216.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
     gene            1904..2500
                     /locus_tag="IXG44_002526"
     CDS             1904..2500
                     /locus_tag="IXG44_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAQ1659217.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAEEKEYMNDADRFRVFG"
BASE COUNT          884 a          495 c          440 g          716 t
ORIGIN      
        1 aaaaacagga actgctgcaa ataagcaatt cctgttttta tgttcattga ttaatgaata
       61 gagcccatta gttttttgat tggttctttc acagtaaata gcaagatacc accgacgatt
      121 gctactgcac cgataatacc gaagtaagca gattctgttc ccggtgtgta gaagcgtgca
      181 atttgtgcat tgattgcttg tgaagaagca tcagctaaga accaaatagc gattgtttgt
      241 gattcaaatg ctcgtggtgc taattttgtt gtcacagaca aaccaactgg tgaaagacac
      301 aattcagcaa taaccacgat gaagaaactt cccaacaacc acaatggact tacttttcca
      361 cttgtaccat aaagcatacc cggcaacatc aatagcaagt acgataatcc tgcaaataaa
      421 agtccaagag agaatttcac tactgtagat ggttgacgtt caccaagttt cgtccacatc
      481 gttacgaata ctggtgtcag aataaagatg aaaattggat tcaatgattg ataccagctt
      541 gcttggatcg ttacaccaaa tagcgtagat tgtgtacgtt cactagcaaa taaagctaag
      601 atagatgaac cttgttcttg taatgaccag aaaatgattg ctgcaaggaa taatgggata
      661 taagcccata ctcttggttt ttcttcagca gttacatctt ttgaagtcag cattttgata
      721 aagtaataca acggtaagat aatccccaat acactgattg tattgacaaa aaagtcaatt
      781 gtcagatgac cagttagttg tgccccgccg aatactgcag cgataacaac aagtgcgatg
      841 atcaagttac ggatgaatcc tttacgttct tcagttgtca aaggattcgg cggtgttgta
      901 cccactccgt ctaatgtctt gcgtccttgg atgatgtatt gtaataatcc aaagaacatc
      961 ccaatagcag ctaaagagaa ccctaaatga tagttatatt cttgaccgat tgtaccaacg
     1021 attagtggtg ctaataatgc ccctaagttg acacccatgt aaaaaatcga aaatcctgca
     1081 tctcttcgca agtcagtctt tgaataaaga tgtccgacca tgccagaaac atttggtttc
     1141 aaaaaggcgg ttccgataat aatcaatgcc atcgaaacaa acaatgtact tacaccaaat
     1201 ggtaaagcca agacgatgtg gccaagcata atgaaaactc caccgaagaa tacagtgcgt
     1261 tttgctccta acactcggtc agatacccat ccgccgacga tactagacat gtagatcaaa
     1321 gatccgtaga tcgacatgat agctaatgct gttgctcgtg gtaatccaag tcccccgttg
     1381 gcaactgtat cgtacatata atagataagt aatgcacgca taccgtaata actaaatcgt
     1441 tcccacatct cagtgaagaa caaagttgac aatcccttcg gctgcccgaa gaagcccttg
     1501 tcttgctgtt tttccataat ctatacccca attctttaaa ttttcagaaa atattataca
     1561 tctttaccga aaaatttaac taaaaaactt atttaagaaa tataaactaa ttttctataa
     1621 aagcaagtaa tttttaatgg caatatcata aaaaaaagaa gatagagatt taataattat
     1681 atatcagctg tttttttcaa ataagcaaaa attcctaaaa cacaacaatg aaagcgataa
     1741 ctaagaaaat ccttacataa cgatttttct tattaaaatt gttttttgaa cttcgcttat
     1801 tttttgtgag catatctgtt tacttacttt ttgtaaggtg ctatacttca tttcgtaatc
     1861 aattaaaaaa atgcaaaacg caaatcaaag gagaaatttt attatgacaa cttttactga
     1921 cactttgaaa aaccgccgtt caatctatca tctaggacgt aatgtttcac tttctaatga
     1981 agaattaaca actttgatca aagaagcaat taaagaaagc ccaactgctt tcaatgctca
     2041 atcaacacgc gcagttatcc ttttcggaga tgcacatgaa aaactttggg aaatcactga
     2101 agaagcactt cgcccattga ctccagcaga agctttccca aatacacaaa acaaattagc
     2161 tggtttcaaa aatggttatg gtacagtatt attcttcaaa gatactgacg tggtaaaagg
     2221 tctacaagaa caatttgaat tatatgcaga caacttccca gattggtcag aacaatcaaa
     2281 cggaatcgca acagctaata cttgggtagc attagttgat aaaggattgg gcgcaaactt
     2341 gcaacattac aacccagtta ttgacgaagc agttgcaaaa gaatggaata tcccttcaaa
     2401 ctggaaacta cgttcgcaat tagtattcgg ttcaccagaa acaccggctg aggaaaaaga
     2461 atacatgaac gatgccgatc gcttccgcgt atttggataa ttttatttga aataaaaaac
     2521 agaggttgtg acaaa
//
LOCUS       DACEUZ010000190         2473 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.190, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000190 DACEUZ010000000
VERSION     DACEUZ010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2473
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.190"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            <1..418
                     /locus_tag="IXG44_002527"
     CDS             <1..418
                     /locus_tag="IXG44_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016204422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1659218.1"
                     /translation="QEDDIIYVTDLTRITRSTQDLFELIDNIRDKKASLKSLKDTWLD
                     LSEDNPYSQFLITVMAGVNQLERDLIRMRQREGIELAKKEGKFKGRLKKYHKNHAGMN
                     YAVKLYKEGNMTVNQICEITNVSRASLYRKLSEVNN"
     gene            632..1327
                     /gene="vanR"
                     /locus_tag="IXG44_002528"
     CDS             632..1327
                     /gene="vanR"
                     /locus_tag="IXG44_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HAQ1659219.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1305..2459
                     /locus_tag="IXG44_002529"
     CDS             1305..2459
                     /locus_tag="IXG44_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAQ1659220.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
BASE COUNT          854 a          443 c          521 g          655 t
ORIGIN      
        1 acaggaagat gacatcattt atgttacaga cttaactcga atcactcgta gtacacaaga
       61 tctatttgaa ttaatcgata acatacgaga taaaaaggca agtttaaaat cactaaaaga
      121 tacatggctt gatttatcag aagataatcc atacagccaa ttcttaatta ctgtaatggc
      181 tggtgttaac caattagagc gagatcttat tcggatgaga caacgtgaag ggattgaatt
      241 ggctaagaaa gaaggaaagt ttaaaggtcg attaaagaag tatcataaaa atcacgcagg
      301 aatgaattat gcggtaaagc tatataaaga aggaaatatg actgtaaatc aaatttgtga
      361 aattactaat gtatctaggg cttcattata caggaaatta tcagaagtga ataattagcc
      421 attctgtatt ccgctaatgg gcaatatttt taaagaagaa aaggaaacta taaaatatta
      481 acagcctcct agcgatgccg aaaagccctt tgataaaaaa agaatcatca tcttaagaaa
      541 ttcttagtca tttattatgt aaatgcttat aaattcggcc ctataatctg ataaattatt
      601 aagggcaaac ttatgtgaaa gggtgataac tatgagcgat aaaatactta ttgtggatga
      661 tgaacatgaa attgccgatt tggttgaatt atacttaaaa aacgagaatt atacggtttt
      721 caaatactat accgccaaag aagcattgga atgtatagac aagtctgaga ttgaccttgc
      781 catattggac atcatgcttc ccggcacaag cggccttact atctgtcaaa aaataaggga
      841 caagcacacc tatccgatta tcatgctgac cgggaaagat acagaggtag ataaaattac
      901 agggttaaca atcggcgcgg atgattatat aacgaagccc tttcgcccac tggagttaat
      961 tgctcgggta aaggcccagt tgcgccgata caaaaaattc agtggagtaa aggagcagaa
     1021 cgaaaatgtt atcgtccact ccggccttgt cattaatgtt aacacccatg agtgttatct
     1081 gaacgagaag cagttatccc ttactcccac cgagttttca atactgcgaa tcctctgtga
     1141 aaacaagggg aatgtggtta gctccgagct gctatttcat gagatatggg gcgacgaata
     1201 tttcagcaag agcaacaaca ccatcaccgt gcatatccgg catttgcgcg aaaaaatgaa
     1261 cgacaccatt gataatccga aatatataaa aacggtatgg ggggttggtt ataaaattga
     1321 aaaataaaaa aaacgactat tccaaactag aacgaaaact ttacatgtat atcgttgcaa
     1381 ttgttgtggt agcaattgta ttcgtgttgt atattcgttc aatgatccga gggaaacttg
     1441 gggattggat cttaagtatt ttggaaaaca aatatgactt aaatcacctg gacgcgatga
     1501 aattatatca atattccata cggaacaata tagatatctt tatttatgtg gcgattgtca
     1561 ttagtattct tattctatgt cgcgtcatgc tttcaaaatt cgcaaaatac tttgacgaga
     1621 taaataccgg cattgatgta cttattcaga acgaagataa acaaattgag ctttctgcgg
     1681 aaatggatgt tatggaacaa aagctcaaca cattaaaacg gactctggaa aagcgagagc
     1741 aggatgcaaa gctggccgaa caaagaaaaa atgacgttgt tatgtacttg gcgcacgata
     1801 ttaaaacgcc ccttacatcc attatcggtt atttgagcct gcttgacgag gctccagaca
     1861 tgccggtaga tcaaaaggca aagtatgtgc atatcacgtt ggacaaagcg tatcgactcg
     1921 aacagctaat cgacgagttt tttgagatta cacggtataa cctacaaacg ataacgctaa
     1981 caaaaacgca catagaccta tactatatgc tggtgcagat gaccgatgaa ttttatcctc
     2041 agctttccgc acatggaaaa caggcggtta ttcacgcccc cgaggatctg accgtgtccg
     2101 gcgaccctga taaactcgcg agagtcttta acaacatttt gaaaaacgcc gctgcataca
     2161 gtgaggataa cagcatcatt gacattaccg cgggcctctc cggggatgtg gtgtcaatcg
     2221 aattcaagaa cactggaagc atcccaaaag ataagctagc tgccatattt gaaaagttct
     2281 ataggctgga caatgctcgt tcttccgata cgggtggcgc gggacttgga ttggcgattg
     2341 caaaagaaat tattgttcag catggagggc agatttacgc ggaaagcaat gataactata
     2401 cgacgtttag ggtagagctt ccagcgatgc cagacttggt tgataaaagg aggtcctaag
     2461 agatgtatat aat
//
LOCUS       DACEUZ010000191         2435 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.191, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000191 DACEUZ010000000
VERSION     DACEUZ010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2435
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.191"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            130..1275
                     /gene="tgt"
                     /locus_tag="IXG44_002530"
     CDS             130..1275
                     /gene="tgt"
                     /locus_tag="IXG44_002530"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010769586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HAQ1659221.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            1372..1752
                     /gene="yajC"
                     /locus_tag="IXG44_002531"
     CDS             1372..1752
                     /gene="yajC"
                     /locus_tag="IXG44_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HAQ1659222.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKKEK"
     gene            2057..>2435
                     /locus_tag="IXG44_002532"
     CDS             2057..>2435
                     /locus_tag="IXG44_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HAQ1659223.1"
                     /translation="MAKVMEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     AQKGLIEIAEPVGVVCGVTPTTNP"
BASE COUNT          891 a          384 c          521 g          639 t
ORIGIN      
        1 ataaaaaaag gataaaaaaa agaatattaa ataaaataga aggaagccga gggaacttgc
       61 aaaaagcact tgaaacgatt atgatggtgg gagttgtcaa gatattttat atagaaagta
      121 gggaaatgaa tgacgcaacc tgccattcgc tatcgtttaa tcaagaaaga aaaacatact
      181 ggcgcgcgtt taggggaatt gatcacgcca cacggaactt tccccacacc aatgttcatg
      241 ccagtaggaa cattagctac agtaaaaaca atgtccccag aagagttgaa agaaatgggt
      301 gctggcgtta ttttaagtaa tacatatcat ttatggctac gccctggtga agacttagta
      361 gaagaagctg gcggactgca taaatttatg aactgggatc aaccgatttt aacagattca
      421 ggcggctttc aagttttttc attaagtgat atgcggaata tcgaagaaga aggcgtgcat
      481 ttcaaaaatc acttgaacgg ttcgcgtatg tttttatcac cagaaaaagc aatcaacatc
      541 caaaataaat tgggctctga tatcatgatg agttttgacg aatgtccacc attcgatgaa
      601 agttacgact acgtgaaaaa atcagtagaa cgtacgtctc gatgggcaga acgaggattg
      661 aaagctcatg ctaacccaga ccgtcaagga ttatttggaa tcatccaagg agcaggattt
      721 gaagatcttc gtcgtcaaag cgcgaaagat ctagtcggga tggatttccc tggttactca
      781 atcggcgggt tatctgtcgg ggaacctaaa tcagaaatga atcgtgtatt agaattcact
      841 acaccgttga tccccgaaaa caaaccacgt tacttgatgg gtgtcggtgc tgctgattca
      901 ttgatcgatg gtgttatccg tggagtagac atgtttgact gtgtcttgcc tactcgaatc
      961 gctcgaaatg gcacatgtat gacttctcaa ggtcgtctag tagtgaaaaa tgcgaagtat
     1021 gctcatgatt tccgtccaat tgatgagaaa tgtgattgtt atacatgtaa aaactataca
     1081 cgtgcctata tccgccattt gatcaaatgt gatgaaacat ttgggattcg tttgacttct
     1141 taccacaatt tatacttctt gttgaatcta atgaaacaag tacgtcaagc aatcatggat
     1201 gataatttgc ttgaatttag agaagccttt tttgaagaat acgggttcaa taaagaaaac
     1261 gcaaaaagct tctaaaggaa aatcttttaa aaattaagta aaaaactagt gttagcagca
     1321 tttttctgct aaagtgtttt ttgtagatac agaattagtg aggtgaaaaa aatgggaaca
     1381 ttaccaatgc ttgtgatgtt tatcgcgcta ttagcaatgt ggttttttat gtcacgttcg
     1441 caaaagaaac aacagcaaga acgtcaaaac ttgttagata gcatgaaacc aggtgattca
     1501 gtcgtaacga tcggcggatt acatggagtt gtttcagaaa tcgacaatga caaacggaca
     1561 gttgttttag actgcgaagg gatcttcctt gagtacgatc gtgcagcaat caaaacggtc
     1621 aaaccaggga ctgtcgtaac aaatgatgca acaactactg ttgaaacaac agaagaacca
     1681 gctgagacag ttgaagtaaa aactgaaaca acagaaacgc tggaagagac agaagagaag
     1741 aaagaaaaat aagtatcaaa gcttatttaa agacgaggta gggcagaaag atgtcctacc
     1801 tcatttttta ttcaaaaaag aaagagataa gagtatagga tatattgctc aatattgaat
     1861 aatcgggtgt gtcatataaa taataataaa taactttata tatttttcta tacaaacgcc
     1921 tataaataaa gcaactttat atttaaaaat aactgtgaaa aaaatcacaa aaaagtgttt
     1981 acatagaaaa ataaatattg tatgatatac atgtgaatga tgtaacaaat aaccatgacc
     2041 cattaggagg ataatgatgg ctaaagtaat ggaaaaagaa aaaacgaaga caattgacgt
     2101 acaagcaatg attgatgatt tggcagaaaa agcaaatgca gcattgaaag aaatggaaag
     2161 ctttgatcaa gaaaaagtgg accatatcgt gcatgaaatg gcaatggcag ctttagatca
     2221 acatatgcca ttagcgaaga tggctgtaga ggaaacaggc cgcggtgttt acgaagataa
     2281 agcaatcaaa aatatgtatg cgtccgaata tatttggcat aatatcaaac atgataaaac
     2341 agttggtgta atcaatgaag atgcccaaaa aggattgatt gaaattgctg aaccagtcgg
     2401 tgtggtttgc ggagtaactc caacaacgaa tccga
//
LOCUS       DACEUZ010000192         2399 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.193, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000192 DACEUZ010000000
VERSION     DACEUZ010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.193"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            208..2070
                     /locus_tag="IXG44_002533"
     CDS             208..2070
                     /locus_tag="IXG44_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1659224.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2070..>2399
                     /locus_tag="IXG44_002534"
     CDS             2070..>2399
                     /locus_tag="IXG44_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1659225.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          866 a          353 c          466 g          714 t
ORIGIN      
        1 aaaaaatttg aaaaagcgtt tgattattta tattacgact tagtgcaata aaaaacgctt
       61 tttaacagta catgtagtcg tctttataat agataaactc tgtaatatta gttaaaaagc
      121 tataagttgc acttagttaa gtgcaaacat ttcatattgt cgttagttta gaaaacgctt
      181 atattagcaa tgtaaggaga gatgataatg cagcagctaa caaaaagaga aaggcagctt
      241 ttgatttact tggtggaaat gtctgaaccg gtaacaatca aaaagttggc tgaaaaagaa
      301 aatgttagtg ttcggacgat aaaatacgat ctagatgata taagagagtg gctgaatgag
      361 cggaagcaag tcctatatag caaacgtagt cagggaattt ggatgaatat tagtagctct
      421 gatcgaatca aattaaaaag cgaattgatg gatgttaatc gttttgaaat atttgcagat
      481 caaagtctcc gaatcaaacg tttactaatt atgcttttag taacaaaaga agctattact
      541 ggaatgaaaa tggccaacgc attagatgtc agcaaagata cgatcatgaa tgatttagat
      601 gtgctggaaa ataattttct taaggaagga ttgaccttga atcgtcaggc gcgaaagggt
      661 ttttggatca taggagaaga acgtctagtt cgattaacga ttgaagagat tcttcaaaaa
      721 gagttcacgg attatgacat ttataaatta atgtcattgt tattgaatgg cggtgagact
      781 gaatactttg aaatgtattc tgctactgct acacctatcc aagaggtatt taatcaagta
      841 atcattcgaa tgcgacactt actggaattt gaaaatctag agaaactgaa ttacgcggaa
      901 ttactaaata ttctgattcg agtgacaatt gctaccgttc gcttacgcaa agaggctacg
      961 attggtcgtt atcaattggt tgctgaacaa gaattagaca aaaacgatct ttcttatcgc
     1021 gtcatgcagc aggtctttga ttattatcag ttgccactgt tcgaggatga atatcattat
     1081 atctatagtg atacattcga cacgattcac cagcaagacg tcatgaaact agttgagaat
     1141 gtgatttata aagtaagtaa agcaatcaac cattcttttt atcaagatcc acaattgttg
     1201 acgaatctgt atgctcattt atccatgcga ttatcgagaa agaaaaaatt tgtcaatgaa
     1261 tataatcctt ttaaagaaga tatcaagcag aaatacccgg aattatttga tgcgatcaag
     1321 caagcagttc gacaagaaat aagtgaacaa aatattctga tcaatgattc tttcgtggca
     1381 tatattgcct tacacttttt ggtttcctac gaaagagaag aagatctacg aagcgttaga
     1441 gccgtctaca tttgttcaac agggttgggt gtgactagtc tgatcaagca aaagatcaat
     1501 gaagaattaa ataatatcac tatcgtggca tttgcttcag tattgaatgc gcaagaaatg
     1561 attgctacca aagatcctga tttggtgatt agtatttttc caattaatgg catcgatcgt
     1621 cctttcatca aagtccatcc attacccact gaacaggact tgaaaaaaat tcaacaaata
     1681 ataaaaaaaa tacttgcttc ttccaatcag caggaaaggc ataacttgaa tcttagagaa
     1741 acgtccatca ataaagatac tttagaagac tttagcaagg aattgatttt gaaagcttat
     1801 acgatttatg aacgtttact tgtcattttg gaagcagaat tgatccaaga atatcgagaa
     1861 gcttttttgc ttcatgtatt cttaatggtt catcgtatca catttgatca acaatatatg
     1921 gcagaaggtc gtcaagtaga ccagattatt ttagacaaag gaacactggt aggaaaaatc
     1981 gaagcagttt ttgcagaaaa taatttagga atcaatcagt cagaaatcag tgcattattc
     2041 tcatatataa aagaaggagc gtcaagttag tgaaaatgtc agacgaggct ttaaaaatta
     2101 tcgaggaaag tcaaaatcgg gaaaagttaa aacaagtgat tgatctaatt gaagagcgat
     2161 tagtagaaca tgctattgta ccaaccgagt tgcagtggac cattctaatt aatcacttga
     2221 atgagatgct gaaacgttct caaagaaagg aaacgattcc cgtagtcgat cgggatttgt
     2281 tcaaagaagt atccaaagaa gccataacta tttctgatga aatagttaga agtatcggta
     2341 atttaaccga tgatgaaata tatgttttgt cgattcattt tgaaacagca aaaaataat
//
LOCUS       DACEUZ010000193         2385 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.194, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000193 DACEUZ010000000
VERSION     DACEUZ010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2385
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.194"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            <1..256
                     /locus_tag="IXG44_002535"
     CDS             <1..256
                     /locus_tag="IXG44_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2156 domain-containing protein"
                     /protein_id="HAQ1659226.1"
                     /translation="SRKSFLQERIAYLVYEFGSHFYSFHGLREYKSKYATNWVSRYTL
                     YSRDSWIAYVMIALLIIDNAPVEQTQGRISGLKKWIRRKY"
     gene            complement(444..1631)
                     /gene="tuf"
                     /locus_tag="IXG44_002536"
     CDS             complement(444..1631)
                     /gene="tuf"
                     /locus_tag="IXG44_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAQ1659227.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            1999..2355
                     /locus_tag="IXG44_002537"
     CDS             1999..2355
                     /locus_tag="IXG44_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ1659228.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
BASE COUNT          727 a          470 c          444 g          744 t
ORIGIN      
        1 ttccagaaag agttttttac aggaaaggat tgcatacttg gtttacgagt ttggttctca
       61 tttttattct ttccatggat taagagaata taaaagcaag tacgctacaa actgggtctc
      121 acgttatact ctatattcta gagatagttg gattgcatat gttatgattg ctttgctgat
      181 tattgataat gcgccagttg agcagacaca aggtagaata agtggtttga aaaaatggat
      241 acgaagaaag tattaaaaac tgcataccga tagatgaaca ctctcatgat gaataaaaga
      301 gactgaagta aaacgaaagt tcatgcttct acaatctctt cttatacaat tgaagaacaa
      361 agagcggtcc aaacagaaat cctgtttgga ccgctctttt attttaaaca tttttcatta
      421 ccaaaatatt cgagtcgcgt ttcttatgct tcgatctctg taacgatacc tgaacctact
      481 gtccgtccgc cttcacgaat tgagaatgtc gttccgtttt caacagcgat tggatggatc
      541 aagtcaacat caatcgtcac gttgtcacca ggcatgacca tttctgtatc ttctggcaat
      601 gtgattgttc ctgtaacatc agttgtgcgg aagtagaact gtggacgata gttgttgaag
      661 aatggagtat gacgtccacc ttcttctttt gtcaacacgt acacttctgc tttgaatttt
      721 gtatgtggag taatggaacc tggtttagca agtacttgtc cacgttcgat atcgtcacgt
      781 tggatccccc gtaacaacac gcctacgtta tccccagctt ccccgtaatc taacgtttta
      841 cggaacattt ctacacctgt tacaactgct ttttgtgttt caggtttgat ccctacgatc
      901 tctacctcat caccgacacg aacagcacca cgatcaatac gacctgaggc aacagttcct
      961 cgacctgtaa ttgagaagac gtcttccact ggcaatagta atggtttatc tgtatcacgt
     1021 tctggtgttg ggatatattc atctactgta tccatcaatt ccataatagc agcttcagca
     1081 tctggatcgc cttgcaatgc ttttaatgcg gaacctttga tcacaggagt gtcatcgcct
     1141 gggaaaccat attcgctcaa taactcgcga acttccattt ctaccaaatc gatcaattct
     1201 tcatcatcga ctaaatcaac tttgttcaag aaaacaatca ggtattttac accaacttgg
     1261 cgtgacaata aaatgtgttc acgtgtttga ggcatcggac cgtcagttgc agaaacaact
     1321 aagattgcac catccatttg tgctgcccct gtaatcatgt ttttcacata gtccgcgtgt
     1381 cctggtgcat caatatgcgc gtagtggcgt ttttctgttt catattctac gtgtgcggta
     1441 ttgatcgtaa tcccacgttc acgttcttct ggagctgcat caatactagc atagtcttga
     1501 ggatttgcta ggcctttttt gcctaatact gtagtgatcg cagcagttaa agtagttttc
     1561 ccatggtcaa cgtgtccaat cgtaccaatg ttaacgtgtg gtttacttct gtcataatgt
     1621 tcttttgcca taatttacaa acctcctaaa taatatagat cactgaaaaa tttcagctct
     1681 caccttttta ttatagtctt tttttgacta aatcaaaaag aatacgtccg acttactttt
     1741 tgtaagattc gaactaagct tattatacgt caatattggt caaagtcaac taagagcact
     1801 cgtctgtctt atacgcgaaa aaagtacctt ttataaataa aaaaatgtgt tttttgagaa
     1861 aaacttcaag atagtcctta actttctttt gaagcaataa gaacaaaatg aggaaaagtt
     1921 taaaattcag ctgttgccaa caactttcaa tagacgaaat cttctatttc gttaataata
     1981 aggaaagagg tgaagtacat gaaaaaatta attggcgtac ttgtcgtttt aggcatattt
     2041 ctcggcggaa gttttgcagc ttatcattat ttttacggcg gcgaagctta ttatacaaaa
     2101 atcacgacga atggcgaaat gtcagctacc caagcagata acggagagaa attcacgact
     2161 tatacctaca agcaagcggc ttataataaa gatggtgaaa aaaaacaggt gacgcttcgc
     2221 gaagaaagag aacatccatt aaagatgaat gcgtatctta aactcaaagt caatcctcgt
     2281 aaaggtgtat taagctggga agaaataaag gcttctgaag tgcctaaaaa tgcggcagaa
     2341 aaaatcgata attagtaaat ggtcttaaaa ttttattaga taaaa
//
LOCUS       DACEUZ010000194         2366 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.195, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000194 DACEUZ010000000
VERSION     DACEUZ010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2366
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.195"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            <1..605
                     /locus_tag="IXG44_002538"
     CDS             <1..605
                     /locus_tag="IXG44_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ1659229.1"
                     /translation="PKGTTYTWKEKVDVSTAGNKKGTVVVTYPDGSKEEVEVTISVVD
                     KKAPNKPQVDPITEGDQIVTGKTEPNAEVTVTLPDGSQHHGTADKNGNFTVKVPKLEA
                     GTKVIVTATDESGNTSEPTNVVVSSNKKDSGKNGSKGSKTDNQGSNSNQDKNRGKSQS
                     SKVFPKTGESDSNIFTISGGLILLGTLGLLGYKNRKKENE"
     gene            739..2082
                     /locus_tag="IXG44_002539"
     CDS             739..2082
                     /locus_tag="IXG44_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ1659230.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPAFLRKNSRVKLRISFEKNTKRIVGVQL
                     CSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFILKAVTGLNIET"
     gene            2194..>2366
                     /locus_tag="IXG44_002540"
     CDS             2194..>2366
                     /locus_tag="IXG44_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ1659231.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSEL"
BASE COUNT          938 a          301 c          422 g          705 t
ORIGIN      
        1 taccaaaagg aactacttat acttggaaag aaaaagtaga tgttagtaca gctggaaaca
       61 aaaaaggtac tgtagtagta acatatccag atggatctaa agaagaagta gaagttacta
      121 tttctgtagt agataaaaaa gcaccgaata aacctcaagt tgatcctatt acagagggtg
      181 accaaattgt tactggtaaa actgaaccaa atgcagaggt gacagttaca ttaccagacg
      241 gaagtcaaca ccatgggaca gctgataaaa atggtaattt tacagttaaa gttcctaaat
      301 tagaggcagg gacaaaagtg atagtaactg caactgatga atctggtaat actagtgaac
      361 ctactaatgt agttgtttct tccaacaaaa aagacagtgg aaaaaacggt agtaaaggta
      421 gtaagactga caatcaaggt agcaacagca atcaagacaa aaatagaggc aaaagccagt
      481 cctcaaaagt ttttcctaag acgggagaga gtgacagtaa tatctttact attagcggtg
      541 gtttaatact gttaggaacg ttaggattat tagggtataa aaatcgaaaa aaagagaatg
      601 aatagaaaat caatttctta agtcttctct gagaagagga agaatctttt tcttcctctt
      661 tttgcttttt tatgatggga aataagtgtt agttaaagat gaatatactt tttatagaaa
      721 ggaaaagaat gtaattaagt ggaaaaaata gtaattattg gttcaaacca ttcagggcta
      781 gccgcagctc gatttctaaa cttaagcgat tcaaattatg aagttattct aatcgataaa
      841 aaatcagaaa ttagttactt ggaatgtggg acatcgttat ttataaaagg agaagtaagt
      901 aatttagaag atctttttta tcaaaataga atgacactaa attctgagat aaaaaagtta
      961 tttctagaga cagaagttga caaaattgat attcaaaata aaaagattag ttgtgtaaaa
     1021 aaagatggta caagattctc tcaagaatat gacaaagtta tattagctac aggttcaaag
     1081 caaattatgc tagatatacc ggggaataat ttagctaata ttttttcgat aaagaatttt
     1141 ccaaatgcaa aagagatttt taaagctttg gatgatagta ctattaaaaa agtaggaatt
     1201 attggagcag gatatattgg agttgaaatt gctgaagcca taagaaaaag agggaaagaa
     1261 gtatatcttt tcgatgttgc agatagagtg ttgtcgactt attacgaccg tagtttttca
     1321 gataaagttg aagaaatact atcaaaaaat ggaatacatc tttgtctatc agaaagtcct
     1381 aaaaaatatt taggaagaaa aaagattgaa aaaatagtca ctacaagtgg tacccaatac
     1441 tctatggata tggtcgtaca agctatagga tttctatcta atagtccaat tggggaaaag
     1501 gaactcttga gagatgttag tggagcatat ctaactaatg aataccagca aacaaatata
     1561 aaagatatat atgctattgg ggattgtgct acaacttttt ttacgtccat taatagaagt
     1621 agtgttgatt tttctatttc taatgctcta agaacagctt atattgcaac tcaacatatt
     1681 aacaatcaag attttacacc ttctggttcc caatttacaa atggcttttc tatatttaat
     1741 tacaattttt acgcagcagg tttgaattta aaaacagcac gattattaag tgagtctata
     1801 gattatatag aaatagaaga tttcattaga cctgcttttt tgagaaaaaa tagtcgtgta
     1861 aaattaagaa ttagctttga aaaaaataca aagagaatag taggtgtaca actttgtagt
     1921 caagaagatt tatctggact attatcaatg ttttctcttg ctatagaaga acaaattaca
     1981 ttagacaaat tgaaaatgtt agattattta tttcatccta gttttagcca accctataat
     2041 tttattttaa aggcagtaac aggattaaac attgaaacat aatcgttact aaatgaaatc
     2101 aagaaatata ctgtattact caattcattg aaaatattta tcataatttt taatagaaaa
     2161 cactattaaa gagaaaaaag agaggtaaat caaatgaaaa aatctttaaa acttgcatca
     2221 gcagttatat taatgtccca actccttata gcaacacctt tccatgtgtt ggcaagcgaa
     2281 aattcaacat acaaaattgc aaaagaaaga accgagacga ctgaaatgag taattccgat
     2341 gtaacagcag agagtagtga actttt
//
LOCUS       DACEUZ010000195         2354 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.196, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000195 DACEUZ010000000
VERSION     DACEUZ010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2354
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.196"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            364..693
                     /locus_tag="IXG44_002541"
     CDS             364..693
                     /locus_tag="IXG44_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007723369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ1659232.1"
                     /translation="MITKTRKQGNSIMLTVPKEFDVPNGVEVEAKLVENGILYEFVEP
                     KKEFFDFSEDILADILSEGYSKQEILKEFRNRKNALTSAFKSIAEDTVANSKPMTKEE
                     LAAEIGL"
     gene            683..970
                     /locus_tag="IXG44_002542"
     CDS             683..970
                     /locus_tag="IXG44_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ1659233.1"
                     /translation="MAYEILPSKHVIKYLKKLKEKTLKEQFLTIIYDEIAVRPHSGEQ
                     KTGDLSGIWAIGFKYAGTTYRVAYEIKDNAVIPILLCGTHENFYEQLKKIR"
     gene            complement(1249..1812)
                     /locus_tag="IXG44_002543"
     CDS             complement(1249..1812)
                     /locus_tag="IXG44_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1659234.1"
                     /translation="MSGYIYGYARVSTRSQELNRQLDLLNEQNCNEILTEKMTGTKAV
                     RPQLNRLKDKIRPGDTVIVESFSRLGRSTKDLIDLVTYFEEHHVKLVSLKENFDTSTP
                     HGRLMMTVFQAFSQFERDLIVERTKEGLKSARARGRKGGRPRVNQKDVDRAVKLYKSQ
                     VYSVKEITEMTGISKATLYRYLKDNRE"
BASE COUNT          828 a          363 c          358 g          805 t
ORIGIN      
        1 ttttttattc catttacttt ttctttatgt acgacagtca cttggcaaac ttttttcatt
       61 catctcacct cacattcaaa cgattatttg aatgaagtat acctatttga tcactctttt
      121 gtcaatctac cttaattcca atacactatt ataagcaatt tcatgtattg aagtaaactt
      181 acttgcaagt gcgtttttga ttccaagtta aaaacatatt attattctac ataatttcct
      241 aatcaaataa aactcctttg aaaattgttc taaaggagtt ttattatttt gtatttctac
      301 taaaaatagc gtacaatgta attactaagt aattacgaaa tgtaaaataa tggaggaaat
      361 aatatgatta caaaaacaag aaaacaagga aattctatta tgttaacggt ccctaaagaa
      421 ttcgatgtgc ccaatggtgt tgaagtagaa gctaagcttg tagagaatgg tatcctttat
      481 gagtttgtag agcctaaaaa agaatttttt gattttagtg aagatatttt ggctgatatt
      541 ctttcagaag gttatagcaa acaagagatc ttaaaagaat ttagaaatag aaaaaatgcg
      601 ttaacttctg catttaaatc tattgctgaa gatactgttg caaattctaa accgatgaca
      661 aaagaggagt tggcagcaga aattggctta tgagattcta ccttctaaac acgtaataaa
      721 gtatctaaaa aaattaaaag agaaaacatt aaaagaacaa tttttaacga taatttatga
      781 tgagatagca gttcgtcctc attcgggaga acaaaaaacg ggtgatttat caggaatttg
      841 ggctattgga ttcaagtatg caggtacgac atatcgcgtt gcatatgaaa ttaaagataa
      901 tgcagtgata cctatcttac tatgtggaac gcatgaaaat ttttatgaac aattaaaaaa
      961 gattaggtaa gaacccaatg tttcaacttg agaggataga cgacacttta aaagataatt
     1021 tttaacagct gagagttgta ataccctata attttaaacc actaacctat atatttatga
     1081 aaaatagaaa aaattaaaac gcattttaag cctctttcaa attaaaagct tcatccttat
     1141 gcacaaagag ggcttaaaat gcgttatttt atattgtttt ttagtaataa taaaaaaatc
     1201 actctaattc tcccataaat attatagatt cctaaagatt aaaatagatt attccctatt
     1261 gtcttttaag tagcggtaga gtgttgcctt gctaatacca gtcatttccg taatttcttt
     1321 tacgctataa acctgacttt tatacaattt tactgctcga tcaacatctt tctggttcac
     1381 tcgtggacgg ccacctttac gacctctcgc acgtgcactt ttcaaacctt ctttggtacg
     1441 ttcaacaatt aaatcacgtt cgaactgact aaatgcttgg aagacagtca tcattaagcg
     1501 tccgtgaggc gtgctggtat cgaagttttc tttcaagctc accagcttaa catggtgttc
     1561 ttcaaaatag gtaacaaggt caatcaaatc ctttgtacta cgacccaatc gagaaaaact
     1621 ttctacaatt accgtatctc ccggacgaat tttatctttt aaacgattta gttggggacg
     1681 aaccgctttg gttcccgtca ttttctcagt taggatttca ttacaatttt gttcattgag
     1741 taagtctaat tgacgattta gttcttgtga acgtgtactc actcgggcat aaccataaat
     1801 gtatccactc atgcttttct tcctttcgta ctttaattct ggcttgattg tatcataaaa
     1861 gggagttata gatacatagt taatgcaaca agttatgata caaaatatga cctcttttat
     1921 ttaataaaag aggtcatttc attttgtctc ataatcgatt gtttttgata cgagtgtgta
     1981 tacgaaataa acaaaaaaat cttaagtaaa tttctagata cctaatttaa tcctaactag
     2041 tgtatcctaa agttaataac gagtttatca caatgaaaat tataaaatgc tcccatatta
     2101 gatataacat gcaatatttg aattagaact acaaaattta ttcgttttat aaagtaaata
     2161 atgttgagat actaaggaat agaatattat aaggaattga gctgaatgta ttggataaaa
     2221 aaagaataac tttattagag atggactata aaaatgctcg taattttctt ctgaagtctg
     2281 aaagctattt gaatgctcag ctaccagaat atattagttt tgacgaaact ttaaaatctg
     2341 ctaaaaaagt ttta
//
LOCUS       DACEUZ010000196         2338 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.197, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000196 DACEUZ010000000
VERSION     DACEUZ010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2338
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.197"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(127..675)
                     /gene="aac(6')"
                     /locus_tag="IXG44_002544"
     CDS             complement(127..675)
                     /gene="aac(6')"
                     /locus_tag="IXG44_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAQ1659235.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
     gene            1228..2331
                     /locus_tag="IXG44_002545"
     CDS             1228..2331
                     /locus_tag="IXG44_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ1659236.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
BASE COUNT          729 a          452 c          415 g          742 t
ORIGIN      
        1 gtacacggtg ttccaaaagt agcttcttcg gaaataagtt ggaatttcac aaaaattcaa
       61 gaagtaattt tcgtaaattc cttcttattt cttgaagctg attacttctg tcacaacctc
      121 tttttattat tgagaatttg gtcgaggaat aatcgttttt gccatccaaa tatccggttt
      181 gtcccagcca tttgcatttg gtaagacacc tacgatttta taacctaatt tttcatagaa
      241 ttcatacgga tgttcacgaa ggttctggat ggaagccact ttatcaaatg tatgctcata
      301 caggtccgtt tgacttaacg ttgttccatg gtctaaatca tccgtaccta aataaatcgt
      361 gattcctcct ctggaagcta cttctttttc taagtaattg actaatcgag tacctatttg
      421 gttctttcgt cgggagcttt ctacaactaa tggatgcaat tcccaacctg tgataccgta
      481 ttgagggatt gcaccaataa atcctactaa ctcatcttgg tctaccgctg ctaccgcgat
      541 tcgttctgga ttcatcattt cttctacttc ttctgccgag ctgtctccat attcttccgg
      601 ccaagtcagt ctcagtaaat cagaaagctg atctttcaat actggattat tacggtcaaa
      661 ttcactgatt atcatacgtc ttcccccttg ttcttgctta ttctaacagt ccttttgaaa
      721 aaacgaaatg ttctcgctca aaaatcttta gctaccaatc agtatctcta ttctttcatt
      781 atttatctta cttataaaat aaaaaaatta tacagaaaca ctttattcaa aataggaaag
      841 atcaggaaag ccatcaattg gaggaaaaaa ctctgtttgt tcatcatgat atagattatc
      901 ttttgcttta aaaaaaacca attatgaatt gacaaataaa acctgttttc atataataat
      961 cttaattttt agaattttta gataattaca aaaaagcaaa gggaatcggt agaaatacta
     1021 agaggaacaa aaattcatta ttgtcgtttt caatgttttt gtacttgttc gtttttagga
     1081 tacttattga tgatttcaag gtgttcatgc caccttattc atcacttatc ctgtcttagg
     1141 ttcacctcta ctccatctac agccgcagtc agatcgatca ctctttaaaa ttttaacccg
     1201 tttcaacgaa gaaagaagga aatgaatatg agtcaaagtg ttttcttacc aaattatacc
     1261 attggaacag atgcgtatga aaaagcaaat ggaatcgtca aagaatatgg gacaaaagta
     1321 gtgctgattg gagggaagac tgcactttca aaggcagaaa acaaaataaa agtagctttg
     1381 acacaggaaa tagaggttgt cgccagtctc tggtacgggg gtaattcaac ttatggaaat
     1441 attgaacagt tgatccagca tgaaagtgtc caaaaagcag acgtcctgtt tgcagtcggt
     1501 ggtggccgtg cttgcgatac tgtaaaagtc attggtgaaa aactagctaa accagtcttc
     1561 acctttccca ctattggttc gaactgttcg cccgtcactg cggtatgtgt catctataac
     1621 gaagaaggcg taatggacgg actcttcttt ccttcaaaac cacctgtaca caccttcatt
     1681 gatacagcaa ttattgccga agcacctgat aaatacttat gggcaggtat tggcgatgct
     1741 ttatccaaag aagtcgaatc tactttttct gctcgtggcg atgatctagg ctacacggat
     1801 agattaggaa tccaaatcgg aaaaatgtgc attgatcgtc tcttgactta tggtacgcaa
     1861 gcattgaaag atagtgccga cgatcgtccc tcatatgcaa ttgaacaagt cgtttcggat
     1921 atcattgtta ctaccggatt ggtttctgtt ttagttaaca ataactataa ttctgctatg
     1981 gcacactcat tctattacgg tacgactgtt ctagaaaaaa tggagggata ccttcacgga
     2041 gcatctgtca gttatggtgt actgacctta ttgatgatgg atcagcagtt cgatttgttt
     2101 gataaaatct atgtgtttat gaaaaagaac caacttccag tatcgttaac cgacatgaat
     2161 ctcaatgtca cgaccgattt agaagcaatc ttagataaag ccatggcaac aaatgatatt
     2221 aaacatgtgc cttatgaaat cacccgagaa aaatttaaac aggccattct tgacgtagaa
     2281 acttatcatg ctacacaaga aagccagcaa ggatataaga aaatcagtta aataattg
//
LOCUS       DACEUZ010000197         2293 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.198, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000197 DACEUZ010000000
VERSION     DACEUZ010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2293
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.198"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(51..1598)
                     /locus_tag="IXG44_002546"
     CDS             complement(51..1598)
                     /locus_tag="IXG44_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAQ1659237.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGRSKETLFEETNGQLNLFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLP
                     ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEK
                     EGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRD
                     NITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKD
                     SQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFF
                     EAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWM
                     ATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHS
                     TSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDV
                     QQHFSRN"
     gene            complement(1700..2053)
                     /gene="tnpB"
                     /locus_tag="IXG44_002547"
     CDS             complement(1700..2053)
                     /gene="tnpB"
                     /locus_tag="IXG44_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAQ1659238.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(2043..2237)
                     /locus_tag="IXG44_002548"
     CDS             complement(2043..2237)
                     /locus_tag="IXG44_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1659239.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
BASE COUNT          644 a          476 c          408 g          765 t
ORIGIN      
        1 tacgtcaaat aacccgacct atagaataga atcgggaatg tttggcgttt ttagttacga
       61 gaaaaatgtt gttgtacatc cttgttccaa ggcaacagtt gatctaaagt ttctgctgaa
      121 agtacgggaa gatttggtat cttatcaaat agaaactcta gatactttac aggattcaat
      181 ccgttaagtt ctgctgtttt atatacactc agaatgattc ctgaagttcg tgctccttca
      241 agacttgtgg aatgcaacca atttttccgt cctatcacca gctctttgat atttcgctcc
      301 gcacgattat ttgtgagttc taaacgacca tcttcaaaaa taggttcaaa accacttcgt
      361 agtttacacg catattgaac ggctgcatcc aacttggatt ttgcaacagg atctatcgtt
      421 gccatccact cataaaaagc aataaataat ggacgcagtt cttgttgccg acagcttaat
      481 cgcttctcag gagaaaagtc tttccatttc ttttctagga caaaccattt atccaattgt
      541 ttgactgctt gagctgcggg aatatccgtg tttttctttc cttttggaat tgcttcgaaa
      601 aatttccgcc gaacatgcgc gaaacaatag acaagtcgta cactttccac ggcattatac
      661 ccgctatagc cgtcacagtg taaataccct gtaaactcct tgagaaaagt tttcggaaca
      721 tctccagcac gactttcgtc gtggtgataa taaacgatgg gatgctgact gtcttttcct
      781 gtacaaaact gccagtaata ggtatctgtt ttggcacttt caatcacacg ataacttgtt
      841 tcatccccgt gtagaatctc ttcttgatta agatagactc ttaatcgttc tcctaaaggg
      901 tctaacgcgt tttgcactgc tttaatatgc caattggtga tattatctct cgaaatatcc
      961 aacccatgtg tttgtttcca atagttctct tgccgatagg caggaacttt ctgaccaaat
     1021 ttcatccgga tcgtttccgc cactaaacta ttggaaccta agctattaga aatcaaaggt
     1081 ttaggaacga tcgctttttt aaaagaagaa agtccttctt tttcgcagct tttacaacaa
     1141 taggtatgtt gataatgggt gagacaatcc aacatagcct gatggaaacg aatttcttct
     1201 cgtacttttg tccgtccgat atctttcatc tgtgaaccgc aatgttcaca aaattgttct
     1261 tccaaaggaa ggagatgttc tttctctgtt ataggtaagt gtttgatttt gtctgctttc
     1321 gttcctacaa ctcgttgatg tccttttact ggaataatgg tggcagctgc ttctggaaca
     1381 cttacagaaa tttcttcatc actaaaaaga ttcaattgcc cattcgtttc ttcaaagaga
     1441 gtctctttag agcgtccaaa taatttgttg gttaaataat tgaccttttc attcaatagt
     1501 tggatttcag ttgtaagatt ctgaatggtt tgttgttgtg cttgattggt tttatgggct
     1561 tcttgaagca actgaagcaa cgtatctgta gtttccaaaa tttttcacca ccttgaatcg
     1621 aactgatttt gttacagaat accacaagaa acgcacaaga aataggggtt caacacattt
     1681 tttgtgttaa ccccccagct taaaaaactc ctttttttcc tggcaaaata gctcgaggtt
     1741 gatcgatact taatccttct aacaaccatc gaacctgttg gttcgttaag gttcgaactt
     1801 cgtctttcgt ccgaggccat tttaacttgc cgttttctat ccgcttataa cagagaagaa
     1861 aaccatcacc gtcccaatag agaagtttga aacggtcttg acggttcccg caaaataaaa
     1921 aaacggcatc ttcgtaaagc tcgagttgat actcttgttg aataagactg gcaagaccat
     1981 caattccttt tcgcaaatct gtttttccgc acacaatata aatatgtttg gtttgaagta
     2041 aatcataaag cattggacaa cagttctttc aaaatagctt gaagaatata attgttaata
     2101 ccgttataga tcaaaacttc agaaccattc tctcttttga tgcgcatcgc taacttggca
     2161 gccgcgggtt tacctttttc tggtgcactc ttttcgtttc ctaattggac aggaacaatc
     2221 tcatttaatt tatacataga aaacctccag acttcttgat agctcaagta tatcttgagg
     2281 tgcatttgtt agt
//
LOCUS       DACEUZ010000198         2248 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.199, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000198 DACEUZ010000000
VERSION     DACEUZ010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2248
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.199"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            <1..2176
                     /gene="adhE"
                     /locus_tag="IXG44_002549"
                     /gene_synonym="adhC"
     CDS             <1..2176
                     /gene="adhE"
                     /locus_tag="IXG44_002549"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HAQ1659240.1"
                     /translation="NPIVFAFHPSAQKSSAEAARVVRDAAIAAGAPENCIQWIEHPSI
                     EATSMLMNHPGIAIVLATGGAGMVKSAYSTGKPALGVGPGNVPAYIEKTAKIKRAVND
                     LIVSKTFDNGMICASEQAVIVDKEIYAAVKAEFQAHQVYIVKPDELQKLEDAVMNEGK
                     YAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAELEGVGPDYPLSREKLSPVLAMIKAN
                     NTDHAFDLCEGMLNLGGLGHTAVIHSENEELHVKFGLRMKACRILVNTPSAEGGIGDI
                     YNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVKTVAKRRNNMQWFKLPPKIFFEKNSL
                     LYLEKMENVERVMIVCDPGMVQFGYCDTVREVLARRKNDVKIEVFSDVEPNPSTNTVY
                     AGTKLMADFKPDTVIALGGGSAMDAAKGMWMFYEHPDTSFFGAKQKFLDIRKRTYKID
                     KPEKTQFVCIPTTSGTGSEVTPFAVITDSETHVKYPLADYALTPDVAIVDPQFVMSVP
                     ASVTADTGMDVLTHAIESYVSVMASDYTRGLSLQAIKLVFDYLEKSVKTPDMESREKM
                     HNASTMAGMAFANAFLGICHSVAHKIGGEYGIPHGRTNAILLPHIIRYNAKDPQKHAM
                     FPKYDYFRADTDYADIAKFLGLKGETTEELVEALATAVYELGKSVGINMSLKAQGVTQ
                     ETLDTTVDRMAELAYEDQCTTANPKEPLISELKQIIIDAYNG"
BASE COUNT          748 a          440 c          483 g          577 t
ORIGIN      
        1 aaatccaatc gtttttgctt ttcatccaag tgcccaaaaa tcttctgcag aagcagcacg
       61 agtagtacgt gatgctgcta tcgctgccgg agctcctgaa aattgtatcc aatggatcga
      121 acatccttcg atcgaagcga cttctatgct aatgaaccat ccaggtatcg cgatcgtctt
      181 agcgacaggt ggagctggaa tggtcaagtc tgcttactct acaggaaaac cagctttagg
      241 tgtaggtcca ggtaacgtac cagcttatat tgaaaaaaca gcaaaaatta aacgtgcagt
      301 gaacgatttg atcgtttcga aaacatttga taatggaatg atttgtgctt ctgaacaagc
      361 tgtgatcgtg gataaagaaa tctatgcagc agtaaaagca gaattccaag cacaccaagt
      421 ttatatcgta aaaccagatg aattgcaaaa attagaagat gcagtgatga acgaaggaaa
      481 atatgcggtc aatccatcaa tcgttggaca ttctgcgatg gaaatcgcaa aattggcagg
      541 aatcagtgta ccaaaaggaa cgaaaatgct gattgctgaa ttggaaggcg taggcccaga
      601 ttatccgcta tcaagagaaa aactttctcc agttcttgcc atgatcaaag ctaacaacac
      661 ggaccatgcg tttgatttgt gtgaaggtat gttgaatctt ggaggattag gacatacagc
      721 agttatccat tcagaaaacg aagagttgca tgtcaaattc ggtcttcgaa tgaaagcttg
      781 tcgtatttta gtgaatacac catctgcaga aggcggcatc ggtgatatct ataacgaaat
      841 gattccgtca ttgactttgg gatgcggttc ttatggtaag aactctgttt ctcgtaacgt
      901 ctctgctgta aacttaatca atgtcaaaac tgtagcgaaa cggagaaata acatgcaatg
      961 gttcaaatta ccgccaaaaa tcttctttga aaagaactct ctattgtact tagagaaaat
     1021 ggaaaacgtt gaacgtgtca tgatcgtttg tgaccctggt atggtccaat tcggttactg
     1081 cgatacagtt cgtgaagtat tggcacgacg taaaaatgat gtgaagatcg aagtcttctc
     1141 agatgttgaa ccgaacccat cgacaaatac cgtatacgca ggaacgaaac tgatggcaga
     1201 cttcaagcca gatacagtaa tcgctcttgg aggtggttct gcaatggacg cagctaaggg
     1261 aatgtggatg ttctatgaac acccagacac atctttcttt ggtgcaaaac aaaaattctt
     1321 ggatatccgt aaacggacat ataagatcga caaaccagaa aaaacacaat tcgtatgtat
     1381 cccgacaaca tcagggactg gttctgaagt aacgccattt gcggtcatca ctgacagtga
     1441 aacacatgtg aaatatccgt tagcagatta tgcattgacg ccagatgtcg caatcgtcga
     1501 tcctcaattc gtcatgtcag ttccagcatc tgtcacagca gataccggga tggatgtgtt
     1561 aacacatgca atcgaatcct atgtttcagt catggcttca gattatacac gtggattgag
     1621 cctacaagca atcaagttag tctttgacta tttagaaaaa tcagtaaaaa cacctgatat
     1681 ggaatcacgt gaaaaaatgc acaacgcttc tacaatggct ggtatggcgt ttgctaacgc
     1741 attcctaggt atttgtcact cagtcgcaca taaaatcggc ggggaatacg ggatccctca
     1801 tggacggaca aatgcgattc ttttaccgca tattatccgc tataatgcaa aagatccaca
     1861 aaaacatgcg atgttcccta aatatgacta tttccgtgca gatacagatt acgcagatat
     1921 tgcaaaattc ttaggtctga aaggcgaaac aacagaggaa ttagtcgaag ctttagcaac
     1981 ggctgtttat gaattaggaa aatctgtcgg catcaacatg agtctaaaag ctcaaggagt
     2041 cacacaagaa acactagata caaccgtaga ccgtatggct gaattagctt acgaagacca
     2101 atgtacgaca gctaatccga aagaaccatt gatctctgaa ttgaaacaga tcatcattga
     2161 tgcttacaac ggataagcaa aagaaagcgt agaaaaaaga taagaaaata gatgaaaaag
     2221 aacttggaaa acgttgtttc tccaagtt
//
LOCUS       DACEUZ010000199         2230 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.200, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000199 DACEUZ010000000
VERSION     DACEUZ010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2230
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.200"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(94..390)
                     /locus_tag="IXG44_002550"
     CDS             complement(94..390)
                     /locus_tag="IXG44_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HAQ1659241.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            746..1102
                     /locus_tag="IXG44_002551"
     CDS             746..1102
                     /locus_tag="IXG44_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAQ1659242.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            1112..2011
                     /locus_tag="IXG44_002552"
     CDS             1112..2011
                     /locus_tag="IXG44_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAQ1659243.1"
                     /translation="MGKLAIISDLHVDIKKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYEPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
BASE COUNT          728 a          380 c          424 g          698 t
ORIGIN      
        1 aaagcaaatc gtagttgtca caatctcttc taaacgatgt tccatcagct gaccttcggt
       61 attagctcaa agcactgatt tcacaacctc tatttaggca tgtgtctcag caagcggttg
      121 atagttgatg atctgcattt ttcgataaaa atcggcggga agcttactcc accgttttaa
      181 taattgatag aaataactct cttctgcttt gattttatac tcgcttaata tcaatccaaa
      241 actattcgtt ttttttgaag ctaatttttt ataaatatcc aatgctttgg ttttatcttc
      301 attgacaaag agtgttttta cttcaatata agaaccctcg atattttgtg tttcttcgat
      361 aaaatagatg ttttttatat attccttcat agtgattcct ccaaagaagt ctttttattt
      421 gataagttta ctctaccgaa gagttgttaa taatccgtga acaaaaagta attctttttc
      481 caatagttgt taaaggtttt ttatttttta tagtattttt tgttatttaa gaaaggaata
      541 aggttaaaca aataacaggt tgctttttgg gtctttttcc gttcaagaaa ctgaacgaag
      601 aaaagggaaa gttgtctctt ttgtgataag agaaatcata ataaacgaat gacttacttt
      661 ccaataaatt gagaacagtc agaaccagac aaattagttg catctgatga ataaagtgat
      721 tattctatga catgaggtga tacagatgaa attagaagtg acaaaagaag cacaagaagt
      781 attgaagaat aaattagaag cagacgatca acttttatta gatatcgaaa acggtgacgg
      841 tccatttgca gaaagccaag tcagttgcca attggacacg gcatttcgat tgatcattgt
      901 caaaaaagat acacaaacag atctaagtct ttattctgta gtggctgata caccagttgg
      961 accaatcaaa atcaaagaca gtgcaatact ttacatggat gatccatcca cacttgcttt
     1021 agaaccaact tataatagtt tacaattaaa aggaccaagc ggcttgagaa aaggcaactt
     1081 gcaagttgtt cgaaaagact agagggataa aatgggaaaa ttggcgatca tcagcgacct
     1141 acatgtggac atcaagaaat tcggcgaatc agagctgatg cagctttggc aagttttgca
     1201 aaagcacaag atcactcggc ttcatctagc cggagatacg gctaataaag tagaccgttg
     1261 cctagctgtt gtcgaatttt tcgtcaataa aggattatct acgacatttc atttcggcaa
     1321 tcacgagttg gcagatgtgc ccaatgaaca gatgatggac cattttccag acccacattt
     1381 tttaaatgaa cggtatattg ctttaaatga gcagaccgtt cttttaggga tgaatggctg
     1441 gtacgactat cagttttccg aattgaaaga ttacgaggca atcgtacgaa tgaagcgttt
     1501 gtattggtat gatcggttca tccagcgtgg gaaaacagat ctcgaggtca atgcagaaat
     1561 gcttgcagct gcaaaaaatt tgctagacca gctggaagca aaaaaacttg aggtgatttt
     1621 atccactcat ttcgttccta aacgggcatt tatcatgtat caatctgctc cttatgagcg
     1681 ctggaacaaa ttaaatgcat ttttaggatc agaatcattc ggagaattac ttgaccgtta
     1741 ttcaaatatc aaacaagtag tttttggcca tacgcatcgt cgctttgaag ataaatccat
     1801 cggtcacacg atttacagct gcagaccttt tggttattat tatgaatggc agctgacccg
     1861 agattttgtc ttgaaagaac agctgttaga tagctatgaa cccatgaaat tacgtgtcct
     1921 cttgcgtgcc catcatccgg cctttcaagc ctatcaaaca caacatttgc aagaggaatt
     1981 tgaacaggcg atgacaatcg ttccgtattg agacagcatt agtaatgtgt aaaagaaaat
     2041 accttgtaaa acataaggat aagtaagagc agtagatcca tttatcgtgg atatgctgct
     2101 ttttttgtgg aaaataatca gcgaatgttt atttgatcgg atgatttgta actcgcagag
     2161 tactagatgc aagcgtttat tctttttttg aacgataatc acaaaaaaaa atataatata
     2221 cattttttat
//
LOCUS       DACEUZ010000200         2176 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.201, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000200 DACEUZ010000000
VERSION     DACEUZ010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2176
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.201"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(37..1356)
                     /locus_tag="IXG44_002553"
     CDS             complement(37..1356)
                     /locus_tag="IXG44_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ1659244.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQTLEFYSKSSEIKVFIKEKNNKNEIQIKDNINVNLES
                     DSNSLIIEEREIKLNDGKKTNLQFVSTADMQKDAKDLSLKFLPYSLLISILFSAIISL
                     IYAKSIKNNIQEIKIVTDKMMKLDKKMSLKVSSNDEVGELKQQINDLYSTLLRTIDDL
                     EFKNKEILKLEKLKYDFFKGASHELKTPLASLKIILENMKYNIGKYKERDIYINECIE
                     IVDSLTKNISQILSVHSIENLNNDEEYLKINDTLEDILKKYEILANQKKITVNNYILD
                     ENVYIGKTALKIILSNLISNAVKYTDVNGVINIGIVNDWLYIENSYGNNKISNMDKIF
                     DVKFDLNKENSNGLGLYIVSNILNNYNIEYKVLQDEEFFIFKIKIFS"
     gene            complement(1353..2006)
                     /locus_tag="IXG44_002554"
     CDS             complement(1353..2006)
                     /locus_tag="IXG44_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1659245.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
BASE COUNT          718 a          331 c          178 g          949 t
ORIGIN      
        1 ctatttttca ataactattg caaaattaca tcttaattaa gaaaatattt ttattttaaa
       61 gataaaaaat tcttcatcct gcaatacttt atattctata ttgtaattat tcagaatatt
      121 acttacaata tataaaccta atccattact attttcctta tttaaatcaa atttaacatc
      181 aaatatttta tccatattcg aaattttatt gttaccgtat gaattctcta tatataacca
      241 atcattaact atcccaatat taattacccc atttacatca gtatatttta ccgcattact
      301 aatcaaatta gatagaataa tctttaaagc tgtttttcct atataaacat tctcgtctaa
      361 tatataatta ttgacagtta ttttcttttg atttgctaat atttcatact tttttaatat
      421 gtcttctaat gtatcattta ttttcaaata ttcttcatca ttattcaaat tttctataga
      481 atgaactgat aatatttgag aaatattttt tgttaaacta tcaacgattt caatacattc
      541 attaatataa atatctcttt ctttatattt tccaatattg tatttcatgt tttccagtat
      601 tattttaaga ctagcaagag gggttttaag ttcatgggat gcacctttaa aaaagtcata
      661 ttttaacttt tctaatttta aaatttcttt atttttaaat tccaaatcgt caattgttct
      721 taataaagta gaatacaaat cattaatttg ttgttttaat tctccaactt catcattaga
      781 gctaactttt aaactcattt ttttatcaag tttcatcatt ttatcagtaa caatttttat
      841 ttcttgtata ttatttttta ttgattttgc ataaattaaa gaaataatag cagaaaataa
      901 aattgatatt aataaagaat atggtaaaaa tttaaggctt aaatctttcg catctttttg
      961 catatcagct gtagaaacaa attgtagatt tgttttttta ccatcattaa gctttatttc
     1021 tctttcttca attatcaaag aattactatc actttctaaa ttaacattta tattatcttt
     1081 gatttgtatt tcattcttat tatttttttc ttttatgaat acttttattt cactactctt
     1141 ggaataaaac tccaaagttt gttctatata ttttatttct tttccattca tattactaga
     1201 aatttcattt gccatattat gaatcttttc ttttctcgtc tccaaatatg ttttaggaaa
     1261 gataaaaaaa attaatgaat gaactaatat aattattatt ccaagaacag aaaatatttg
     1321 tatgaacatt tttggaaata tctttaattt tttcattttc tctccaattt atatcctaca
     1381 tttcttatag tagttataca gtctaattgt aatttttttc taagttcttt tatatataca
     1441 tctattactc gatcataagg agtttcttcg ctatctttcc atacataatc aataatttgc
     1501 attcttgtta acacttgtcc attattatcc aataaacatt ttaatacttc tagttctttt
     1561 gcatttacat ctatttcttc accatttatt ttagctgtat agctattaaa attaaccgat
     1621 agatccttat attcaaactt ctctaaatgt ccataattct tcttaattaa agaatctatt
     1681 ctagctttta agaccggcaa tgaaaatggc ttttctacat atccgtctac taaattagta
     1741 aatgcatcaa ttttatattc ttcatcacta aatgcagtca agattagaat tggtaaattg
     1801 cttttctttc taatttcttt caacacttct aaaccattta taaaaggtat ctgaatgtct
     1861 aagataacca aatttatatc gctattaaat tttgacaagg cttctcttcc atctttagct
     1921 tgaataacag tatatccaaa ttctgaaaga tattcactta ttccctctct tatcatatta
     1981 tcatcttcaa cagttaatat tttcatatat acctccactt aagcctatac actagatatt
     2041 atacagcata ttatggctct ataatatcta gtgttggtga cgccttctgt tgccaatact
     2101 ttttcttttt tttaataaat gcaaacagac accttgaaat cctttagaat aaagggttct
     2161 ataatatttt gtgttg
//
LOCUS       DACEUZ010000201         2155 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.202, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000201 DACEUZ010000000
VERSION     DACEUZ010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2155
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.202"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            26..985
                     /locus_tag="IXG44_002555"
     CDS             26..985
                     /locus_tag="IXG44_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ1659246.1"
                     /translation="MTYTHLTPTELVMIEAYFNQSHSVSKVAHLVQRSRQTIYNVYRF
                     LKSGGSAISYYEQYKKNKRNCGRRPIVLPDGQQEYIQKKVAQGWTPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDPHDLPMKGKRKPNGHKEKRGKQAFRRSIQDRTTDYIQFNHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPAGRQAIDIENRLNQWFQSVPKHLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNK
                     VSETFIQSVASKRNHIPRKSLHYRTPLEVFLSYVGKDDLSSLI"
     gene            complement(972..1577)
                     /locus_tag="IXG44_002556"
     CDS             complement(972..1577)
                     /locus_tag="IXG44_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1659247.1"
                     /translation="MINSAYFHIKYLKKYEYEEVVLDYGLNRQSFLIYAINNGNLPSN
                     KKNIIIETYAYLEGGSKKLIKSCNIEYQLSVGEIKCVFEEDLELDLKKIFASIHDYIG
                     LIYKIIEDDKQTELNMCYFDREQDFFYYGGMGGGSIVPKINLVELDVNNYRELYVFNS
                     LDFEIPMKQMKNIDIHILPSESCQIEYSIEYFYKVSFVKLS"
     gene            complement(1817..>2155)
                     /gene="tnpB"
                     /locus_tag="IXG44_002557"
     CDS             complement(1817..>2155)
                     /gene="tnpB"
                     /locus_tag="IXG44_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAQ1659248.1"
                     /translation="IKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSSFVSKLQYKAD
                     WYGRKIIKVDKWFPSSQICSNCGHKDGKKSLEIRKWTCPICHTHHDRDINASINILTE
                     GLRLHSMGLA"
BASE COUNT          705 a          387 c          350 g          713 t
ORIGIN      
        1 aaagaattat aaggagtgag tacaaatgac ctatacccat cttacaccaa ctgaactcgt
       61 aatgatagaa gcttatttca atcaatcaca ctctgtttca aaagtagccc atctggtaca
      121 acgttctcga caaaccattt ataatgtcta tcgttttctt aaatctggtg gaagcgcaat
      181 aagctactat gaacaatata agaaaaacaa acgtaactgc ggaagacgtc cgattgtgtt
      241 accagatggg caacaagaat acattcaaaa aaaggtggct caagggtgga ctccagatgt
      301 ccttattggt cgagcagagt ttcctattgc ttgttcggta cgtacactat atcggatgtt
      361 taaagataaa acctttgacc ctcacgattt accaatgaaa gggaaaagaa aaccaaacgg
      421 acataaagaa aaaagaggaa aacaagcttt tcgccgttcg attcaggata gaacaactga
      481 ttatatacag ttcaatcatg agtttggtca cctagaaggt gacaccattg tcggtggaaa
      541 acataaaagt gcggtgatca cgttagttga gcgattatct aaagtgatca tcaccttgaa
      601 acccgctggt aggcaagcta tagatattga gaatagactg aatcaatggt ttcaatctgt
      661 accgaaacac ttgtttaaat ccatcacatt tgattgtgga aaagagttct cgaattggaa
      721 aaagattagt aataccaatg atatttcgat ttatttcgct gatccaggaa caccttctca
      781 acgaggacta aatgaacatt cgaatggctt attacgtaaa gatggcttat ataaagctat
      841 ggattttaat aaggtatccg aaacctttat ccaatcggtg gcatcaaaac gaaatcatat
      901 tcctagaaaa tcattacact atcgcacacc attggaagta tttttgagtt acgtaggtaa
      961 agatgatttg tctagcttaa tttgacaaac gaaactttgt aaaaatattc gattgaatat
     1021 tctatttggc acgactcact tggcagtata tgtatgtcaa tatttttcat ctgtttcata
     1081 ggtatttcaa aatctaaact attaaaaaca tataattccc tgtaattatt tacatctaac
     1141 tctactaaat ttatctttgg aacaatagat cctccaccca ttccaccata ataaaaaaaa
     1201 tcttgttctc tatcaaaata gcacatatta agctcagttt gtttatcatc ctcaataatt
     1261 ttataaatca aacctatata atcatgaatg ctggcaaaaa ttttttttaa gtcaagttct
     1321 aaatcttctt cgaaaacaca tttaatttct cctactgata attgatactc tatattacat
     1381 gacttaatta gttttttaga tcccccctcc agataggcat atgtttcaat aattatgttc
     1441 tttttattgc ttggcaaatt tccgttattt attgcataaa ttaaaaatga ctgtctattt
     1501 agcccataat ctaatacaac ttcttcatac tcatatttct ttaaatattt aatatgaaaa
     1561 tatgctgaat taatcaaaac tcgagtgtta ctgttgttat ttattgttgt tctattgtgt
     1621 ataaaattgt ataggttgtc atattttctt ggtttagtga actactcacc acttagctaa
     1681 acctaacggt ttcttacgtt tgaagtggga gcttcttggg aagagagcat acttgatagc
     1741 gtattacttt actaacagag gtgcctctta tttaccaagc tatccccgta gttcctacgg
     1801 ttcttgttta tttgatctaa gccaatccca tagagtgtag ccttagacct tcggtcagga
     1861 tattgatact agcgttgata tctcggtcat gatgagtatg acaaataggg caagtccatt
     1921 ttcgaatttc gagagatttc ttgccgtctt tatgtccaca attcgaacag atttggctag
     1981 atggaaacca cttatctact ttgatgattt tccgaccgta ccagtcagcc ttgtattgta
     2041 atttagacac aaaactagac caagaaacat ccgaaatact ttttgctagt ttatgattac
     2101 gcaacatgcc ttttgtgttt aagtcttcaa tacagatgat atcgtgattt ttgat
//
LOCUS       DACEUZ010000202         2131 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.203, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000202 DACEUZ010000000
VERSION     DACEUZ010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2131
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.203"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(<1..71)
                     /locus_tag="IXG44_002558"
     CDS             complement(<1..71)
                     /locus_tag="IXG44_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ1659249.1"
                     /translation="MKYKEMFVPSLIQLAVEVKNKEEA"
     gene            295..1086
                     /locus_tag="IXG44_002559"
     CDS             295..1086
                     /locus_tag="IXG44_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ1659250.1"
                     /translation="MLKKERQEQIITLLEKDFYLTNSEIANKLGVSEMTIRRDVTELA
                     KKNKVKKLYGGVERKEPYNRELSTQEKIDTNVAEKKYIGQVMNSMIPDNSTIYVGAGT
                     TILYALPFIQKKNLFVITNSLIAFNYLKNHTSYRILLTGGEFSPITEEFIGEVAERSF
                     ESLNVDISFAATNGIFDNNVTTAQFIEGNIQNAALNAAKIKCIVADYSKLNKSDVYTF
                     RKLTDFDYLITDNHLSEQDFNHYSKYLTILKEPKNDTDNHDEPIH"
     gene            1052..2005
                     /locus_tag="IXG44_002560"
     CDS             1052..2005
                     /locus_tag="IXG44_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HAQ1659251.1"
                     /translation="MILTITMNPSIDSIYFTKHFELGTMTRFDSPVRYVGGKGINCGR
                     ASALLGSSVILTGFLGGYFGSMIQDMLKNENLFDLHFLPIAEESRGAVTIMHDDETQT
                     ELVEAGPEIADTTIKKLMNDIKLISKNNYLSVITINGSVNNPDPFLYAKLLQYIKEQI
                     DPSTYILMDISGQHLREVLASKYKPMFIKPNIHEFSDIIHQKITQKSDIKHHLKTNPL
                     FKDIDVIMISCGSYGAVVKVKEEFWNVEIPKIPIINTTGSGDATVGGFAHSLEQNYPL
                     KECVRYAMACGMSNAQQSEVGKVIPADVKNLAKSIKITEMK"
BASE COUNT          772 a          346 c          348 g          665 t
ORIGIN      
        1 gcttcttctt tattttttac ttctaccgct aattgaatca aactaggaac gaacatctct
       61 ttatatttca ttccaacctc ctcctttttg attaagtttg ttaatttatt gttctactaa
      121 gttcatcttt gttttacaag aacattatat ttctgttagt ttgttttgtc aacgctttca
      181 aaagaaattt attttgttaa atttgatcta cttgaaaata aaatcgttta tctatataat
      241 taagaagaac aaagtcgaac aaaaattgac ttttatgtta aaggagtaat tcatatgctg
      301 aaaaaagaaa gacaagaaca aatcattaca ttattagaga aagattttta tttaactaat
      361 tcagaaatcg ctaacaaatt aggcgtatca gaaatgacca tacgccgaga tgtcactgaa
      421 ttagcaaaaa aaaataaagt aaaaaagcta tatggtggcg ttgaacgaaa ggaaccttat
      481 aatcgagaac tatcgactca agaaaagatt gatacgaatg tagccgaaaa aaaatatatt
      541 ggtcaagtca tgaacagtat gattcccgat aattctacta tttatgtcgg tgctggaaca
      601 acgatcctat atgcacttcc tttcattcaa aagaaaaatc tatttgtaat tacaaacagt
      661 ttgattgctt tcaactattt aaagaatcat acaagttatc ggattttact tactggtggc
      721 gaattttctc ctattacaga agagttcatt ggggaagttg cagaaagatc ttttgaaagt
      781 ttgaatgtcg atatctcttt cgctgcaaca aacggcatat ttgataacaa tgtgacaacc
      841 gctcaattta ttgagggaaa tattcagaac gcagcgctca atgcagcaaa aatcaaatgc
      901 attgttgcag attattcaaa attaaataaa tcagacgtct atacttttag aaaattaacc
      961 gattttgatt atctaataac cgataatcat ttatcagaac aggatttcaa tcattattca
     1021 aaatacttaa caatattaaa ggagcccaaa aatgatactg acaatcacga tgaacccatc
     1081 cattgattcc atttatttta caaaacactt tgaattaggt acgatgacac gctttgattc
     1141 tccggtacgc tatgtaggcg gcaagggaat caattgcgga cgagcttctg ctctactagg
     1201 ttcatcagtt attttaacag ggtttttggg cggttatttt ggcagtatga ttcaagatat
     1261 gctgaaaaat gaaaatttgt ttgatttgca ttttttacct attgcagaag aatcaagagg
     1321 tgccgtcacg atcatgcatg atgatgaaac gcagacagag ctagtggaag ctggtcctga
     1381 aatagcagat actactatca aaaaattaat gaatgatatc aaactaatca gtaaaaataa
     1441 ttacctttca gtcattacaa taaatggttc tgttaacaat ccagatccct ttttgtatgc
     1501 taaattatta caatatatca aagaacaaat agatccttcc acctatatat taatggatat
     1561 atctggacaa catctccgtg aagtcttagc aagtaagtat aaaccgatgt tcatcaaacc
     1621 taatatccat gagttttcag atatcatcca tcaaaaaatt actcaaaaaa gcgacataaa
     1681 acaccatcta aaaacaaatc cactatttaa agatatcgat gttatcatga tttcttgcgg
     1741 ctcgtatggt gcagtagtca aagtaaaaga agaattttgg aatgttgaga tccctaagat
     1801 tcctatcatt aatacaacag gctctgggga tgccactgtt ggaggttttg ctcattcatt
     1861 agaacagaat tatcctttaa aagaatgtgt aaggtacgca atggcttgcg gcatgtcgaa
     1921 tgcacagcaa tcagaagtcg ggaaagttat tccagctgat gtaaaaaatt tagcaaaaag
     1981 cattaaaatc acagaaatga aataaatagt acaaagtaaa taattgttta tatgaaatga
     2041 gacacaaaga gactgtgaca aaaaacgtgt gtaccgaccc ccaaaagtta gattttttgg
     2101 tctaactttt gggagtcgat acattgtcac a
//
LOCUS       DACEUZ010000203         2112 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.204, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000203 DACEUZ010000000
VERSION     DACEUZ010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2112
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.204"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(757..1296)
                     /locus_tag="IXG44_002561"
     CDS             complement(757..1296)
                     /locus_tag="IXG44_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAQ1659252.1"
                     /translation="MQPKETFTIKELSELFEISRQAISKHVDKLDPSLLAKNERGYKV
                     VLRSGVLQLASNLGNQRLLYRLHEYEQENDKNVYISSKNVIVNELLDKLREENEFLRE
                     QLSLKEQNNSNMQKLLDQQQQLTLQANQQIEKLQEQLQLAYTEETPEDESTTILSEET
                     ESIEKHTKKKKQKWWQLWK"
     gene            complement(1324..2061)
                     /locus_tag="IXG44_002562"
     CDS             complement(1324..2061)
                     /locus_tag="IXG44_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015060391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAQ1659253.1"
                     /translation="MSNDIVRYNNGLNSVPLRKFTPIDMDLFWGICSKMKRKGTKEEI
                     FTFKQIKELIDYNINARGDRFANDLIKMSKKIITLSFIYEDEKTYEQLNLFQKFKIDK
                     VNETLLIKVSEEFEYILNSIGTNFTRFELENMTNLKSSYVKELYRQLMAHKDRRSKKG
                     HWFVKIEDFRALLAIPESYRMRDIDKQIFAQAKKEFLNVNSTHPAIFKTFEIKKIKAY
                     KGNKIGSLQIYFEENIPSITLDNWLEK"
BASE COUNT          625 a          313 c          289 g          885 t
ORIGIN      
        1 aaaaaataaa aaaaataatt tactggtatt gacagctatt atttttaata tatttgttac
       61 tatattaggt ttatttgaat tgattcaaat gaattttttt agctttacac atggaatatt
      121 tacttggttt gctctatctg gattaatagt aatagatatc ttaaattatt tttatattta
      181 ctattcttat ttaaaaaatg aaaaattttt ttatacaatg tttttccccc tagttatttt
      241 gattatttta ttaagctaat atgaagtagt tagttattta attgcatagt tgacataatg
      301 tacactatgc caagtagccc ctctaccaat ggatagagac gctcactttg tgatttttaa
      361 ttggtctagc taaaagcaac tttctaaagc ttgatttttc gcggttatct atatctaatt
      421 ctttgtgcat ttttatctgt cattttttta agacttgtta ttttttgtaa aatatggtaa
      481 atttttggta gcgcttcata agcgcgtgct ttttcaagaa taaacactca ttatttttta
      541 actggctgct cggaagagca gtctttttta ttatttatcc gtctctttta caaacaattc
      601 ttcgttagga ctgttgtaat aatttggatt caatttcaat tcaccatgtt tattcccttt
      661 attgattact gacaataaca tcggactgaa gcctagaatt cctagtaaaa tagaaccgaa
      721 tagaagattt tttcttgtca tgaatgttcc ctccagttat ttccaaagtt gccaccattt
      781 ttgcttcttc ttttttgtgt gtttttctat cgattctgtt tcttcagaaa gaatagtagt
      841 tgattcatct tctggagttt cttccgtata agctaattgt aactgttctt gcagtttttc
      901 gatctgttga tttgcctgta gggtgagctg ttgttgctgg tcgagtaatt tttgcatatt
      961 agaattattt tgctctttga gggaaagttg ttctcgtaaa aattcatttt cttcacgcaa
     1021 cttatctagc aactcgttta caatcacgtt tttgcttgat atataaacgt ttttatcgtt
     1081 ttcttgttca tactcatgca acctatatag taacctttgg ttacctaagt tgctagcaag
     1141 ttgcaatact ccagaacgta atacaacctt ataaccacgc tcatttttag ctaataagga
     1201 cgggtctaat ttatcaacat gtttagagat tgcttgccta cttatttcaa ataattcaga
     1261 taattccttt attgtaaagg tttctttggg ttgcatgata attgcctcct aaattgttag
     1321 gtattatttc tctaaccaat tatctaaggt aattgaaggt atattttctt caaaataaat
     1381 ttgcagagat cctatcttat ttcctttgta ggctttaatt tttttgattt caaaagtttt
     1441 aaaaatagca gggtgagttg aattaacgtt taaaaattct tttttcgctt gggcaaaaat
     1501 ttgtttatca atatctctca tgcgataact ttctggtatt gctaatagag ctctaaaatc
     1561 ttctattttt acaaaccagt gacctttttt agatcttctg tctttatgag ccataagttg
     1621 gcgatataac tctttaacgt agctactctt taagtttgtc atattttcta attcgaaacg
     1681 agtaaaatta gttccgattg aattaagtat atattcaaat tcttcagaaa cctttattaa
     1741 taatgtttca ttcactttat cgattttaaa tttttgaaac agatttaatt gttcatatgt
     1801 tttttcgtct tcataaataa aagacaaagt aatgattttt tttgacattt ttattaaatc
     1861 attagcaaaa cggtcgccac gtgcatttat attgtaatca attaattctt ttatttgttt
     1921 aaatgtaaaa atttcttctt ttgtaccttt tcttttcatt ttagaacata ttccccaaaa
     1981 taaatccata tctatagggg taaattttct taaagggaca gaatttaatc cattattata
     2041 tcgaactata tcgttactca ataaaaatca cctcaataaa ttatgttatc atagacataa
     2101 gaggatgtaa aa
//
LOCUS       DACEUZ010000204         2056 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.205, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000204 DACEUZ010000000
VERSION     DACEUZ010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.205"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            493..714
                     /locus_tag="IXG44_002563"
     CDS             493..714
                     /locus_tag="IXG44_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1659254.1"
                     /translation="MRVIKSEKFTLAEIMTTSELAQELDYNQAYVLRLAKEHLTEGKE
                     YRSAGRRNYLFSPEALKKLEKVITTKEGE"
     gene            715..972
                     /locus_tag="IXG44_002564"
     CDS             715..972
                     /locus_tag="IXG44_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1659255.1"
                     /translation="MPKETTINVEQLEKLLESYSNDFIELTGGKVEETTDLFDLLLTN
                     ILELNSEEQKEHKGKLIAPSYRKAAKSAVPTGFLTKQPYIF"
     gene            complement(1108..1710)
                     /locus_tag="IXG44_002565"
     CDS             complement(1108..1710)
                     /locus_tag="IXG44_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAQ1659256.1"
                     /translation="MTSRKTKDERTRNWTFVVYPESAPENWREFLDELHVPWVESPLH
                     DKDVNPDGELKKPHWHVLIMFSGKKTYDQIREITQKIRSPNPQKCANAKGMVRYFAHM
                     DNPEKFQYAKSDIIAHGGAEIASYLSVTSAERYELIREMMSFVDSKNITEIKDLIDYA
                     MSERFDDWFPLLCDNSAYIIGQYIKSNRHGGSVNSKINKG"
     gene            complement(1777..1932)
                     /locus_tag="IXG44_002566"
     CDS             complement(1777..1932)
                     /locus_tag="IXG44_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010001600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ1659257.1"
                     /translation="MISNEKKRVVVTLTKEVADNLDKIAKQMGLSKSGLITIWVNANG
                     KENEQKK"
BASE COUNT          678 a          464 c          313 g          601 t
ORIGIN      
        1 aaaaaaaaac gctgaaaccc ttgggagagt aaggctaaac agctatcgac caatgaccaa
       61 tgaccaataa ggggggtgtc gtagtccccc cctcctccgc ccaattcata aacaccaaaa
      121 ccaaaaaaca aacctcaggg ctccgccccg aaccccgacc tccttctacc gaaatatatg
      181 caggcgccac gcttactatt tcgttcagct atcggctttt gcaatctaac acgaccgttt
      241 cgctaccagt cgccttacag gctccattct cgcttctctt acgtgtttta acgattgcgc
      301 cgataactaa acgaaataga cgctaaatcg cttaaaacgc aaaatagacc ctttttagag
      361 cattgatttt ttctactagt catttcgata atataaaatt gaccacaaca cagaaaactt
      421 atattataag cttatgtcgt aagtccgacg tactcacgta atatagacgg acgttaaatg
      481 gaaggagcat aaatgagagt gataaaatca gaaaaattca ctttggccga aattatgacg
      541 accagcgaac ttgcacaaga acttgattac aaccaagctt acgttttgcg cttagcgaaa
      601 gaacatctga ctgaaggaaa agaatatcgc tctgccggta gaagaaacta tcttttcagc
      661 ccagaagccc ttaaaaaact agaaaaagtc ataaccacca aagaaggtga gtaaatgcct
      721 aaagaaacaa ctatcaacgt tgaacaacta gaaaaattgc tagaatcata ttccaatgat
      781 tttatcgaat tgaccggcgg aaaagtcgaa gaaaccaccg acctctttga cctacttctc
      841 acaaatattc tggaattaaa ctctgaagaa caaaaagagc ataagggaaa actgatagca
      901 ccttcctaca ggaaggcggc taagtcggct gtgccaactg gttttcttac aaaacaacct
      961 tatatttttt gatatcacaa aaaaatccat tttggaccta ttttcggtca taatatagta
     1021 cctacttttg gtcatagttt cgtccgtagt tttggtcata gtgactaaga ctttcacgca
     1081 caaaaaaaga gctgaaatta acagctctta tcctttattt atttttgaat ttacacttcc
     1141 accatgacga ttcgatttta tatattgccc gataatataa gctgaattat cacaaagaag
     1201 cggaaaccaa tcatcaaatc tttctgacat tgcatagtca attaaatctt tgatttcagt
     1261 gatattctta gaatcaacaa acgacatcat ctctctaatc aactcatatc gttctgcact
     1321 cgttactgac aaataactag caatttctgc accaccatga gcgataatat cacttttagc
     1381 atattgaaat ttttctggat tatccatatg agcaaaatat cgcaccatac cttttgcatt
     1441 agcacatttt tgcgggtttg gagaccgaat cttttgagtt atctcacgta tttgatcata
     1501 cgtcttttta ccgctaaaca ttatcaaaac atgccaatga ggctttttca attcaccgtc
     1561 aggattcaca tctttatcat gaagtggact ctcaacccaa ggaacatgta actcatctaa
     1621 aaactccctc caattttcag gagccgattc aggatagact acaaacgtcc aattccttgt
     1681 gcgttcatct ttagtttttc tactcgtcat gctataatca ccttaattga acaacagtct
     1741 aacagcaaaa gcagtgcgcc aacactaatg cttttgctat tttttttgct cattttcctt
     1801 tccgttagca ttaacccaaa ttgtaataag accagactta ctaagaccca tttgtttcgc
     1861 aattttatca agattatctg ccacttcctt agtaagcgtt acaacgactc tctttttttc
     1921 attactgatc ataattcgta tcctccaacg actttttctt aaaaaccata gtaacaaaaa
     1981 agtaactaag gtgcaacagt ttagatacta attagcgaat aataggcctt aaacccttgg
     2041 cattactgat tttgtc
//
LOCUS       DACEUZ010000205         2034 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.206, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000205 DACEUZ010000000
VERSION     DACEUZ010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.206"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(63..1403)
                     /gene="glnA"
                     /locus_tag="IXG44_002567"
     CDS             complement(63..1403)
                     /gene="glnA"
                     /locus_tag="IXG44_002567"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HAQ1659258.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(1431..1823)
                     /locus_tag="IXG44_002568"
     CDS             complement(1431..1823)
                     /locus_tag="IXG44_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ1659259.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT          578 a          451 c          342 g          663 t
ORIGIN      
        1 gtttctctaa tactcaggtc aaaacgctga tttcacagcc tctaattcac ggtattcctg
       61 ttttaataaa gttctagata ttgttctctt tcccactcgg aaacagtttg tctgaatgcc
      121 gcccattcga gacgttttgc ttcaacaaaa tttgagaaga tatgttgtcc taatgcgtca
      181 atcattacat tatccttacg caattctttg atagcattat ggatagttga aggtaagtcc
      241 tggatatgtg cagcttctct ttcttcctca tccattacat aaatgttacg gtcaacagct
      301 gctggcggtg tgatctgatt acggataccg tctagtcctg cttgcaacaa tactgccatt
      361 gccaagtatg ggtttgcaga tggatcaact gaccgcaatt ccaaacgtgt ggataaaccg
      421 cgagattctg gtacacgtac taatggcgaa cgattacggc cactccatgc gacataaaca
      481 ggcgcttcat aacctggaac gagacgttta taagaattga ctgttggatt gcaaacggct
      541 gtgtaggcac gagcatgttt cagcaaacca cctaagaaat gataagctgt ttcacttagt
      601 tccaattcac cgtctttatc atagaaaaca tttccttcat cattgaacaa agacatattg
      661 caatgcatac cagaaccatt gatcccaaat aacggtttcg gcatgaaagt cgcgtgaagt
      721 ccatgcttgc gtgcaatcgt tttgacaacg agcttgaagg tttggatatt atcacaagca
      781 tctaccacat ctgcatattt aaagtcaatt tcatgctgtc ctggcgctac ttcatgatgt
      841 gaagcttcca cttcaaagcc tagactttct aattccaaca cgatgtcacg gcggcaattt
      901 tcacctaaat ctgttggtgc aaaatcaaaa taaccacctt tatcattcaa ggtcgttgtg
      961 atcccaccgt cttcgtctaa tttaaataag aagaattctg gttcaggtcc taaattgaaa
     1021 gaggtaaaac caaggtcgcg catatctttc aatgctcgtt tcaaattccc gcgaggatcg
     1081 ccggcaaatg gtgtcccatc cggattatag atatcacaaa ttaaacgagc aacttttcca
     1141 tgagcacttt cccacgggaa gatcatccaa gtggagagat ctggatacaa atacatatcg
     1201 ctttcttcaa ttcgaacaaa tccttcaatc gaagaaccgt caaacatcat tttattttct
     1261 aatactttgt cgatctggct cactggaact tctacatttt tgatcgtacc taagatgtct
     1321 gtgaacatca atcgtaaaaa tcgtacgttt tcttcctcaa tgattcgttt gatttcttca
     1381 cctgttatct gtttctttac catggtatcc ctcttccttt tttaatttat tcagtcaaag
     1441 aattgtcttt gcaatccttg cgactgataa tgacctgttt tgcttaaccc accttgcgcc
     1501 aaaatctcat cacgaaaaat ttgtcgaacg tcatgatctg tcaacggctt cttgtttttt
     1561 gcttcttgtt ttttcgcctg tgccatttcg tatacacgtt tgatcccagc catattcaat
     1621 ccctctgaaa gatagtcttt aatctcaagc aacatatcaa tatcattcag cgaatacata
     1681 cgtcggtttc cttcgcttcg ttccggatga actaaatctt gttcttcata atagcgaatt
     1741 tgccgagcag acaaatcagt cagcttcata actgtaccaa ttggaaatac ggacatagat
     1801 cggcgcaatt ccttttctcc catcagacgt cccctttcgt tttataagtt ggctaaagtg
     1861 taccaattaa aaaaaatatt gtcaactgca tatgttaggt tttctaacat gagatctcta
     1921 ttttttataa aataacaaaa aaataagaac acaaaaaagg tatctagttg attttcgtac
     1981 cagatacctt ttatttttta ttatactgta ctatccttta ttagtgtttt tatt
//
LOCUS       DACEUZ010000206         1877 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.207, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000206 DACEUZ010000000
VERSION     DACEUZ010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1877
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.207"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            153..1313
                     /locus_tag="IXG44_002569"
     CDS             153..1313
                     /locus_tag="IXG44_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ1659260.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            1329..1772
                     /locus_tag="IXG44_002570"
     CDS             1329..1772
                     /locus_tag="IXG44_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1659261.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
BASE COUNT          652 a          300 c          375 g          550 t
ORIGIN      
        1 aacaaaaaat atgagtagct atttttttgg gctgtactta ttacttgatg caggacagct
       61 ttttcacaac ctcatcggct ttcattttct atttttttcc tgaacccata taataaaaga
      121 aagaacaaaa aaacttgcaa gggaggacac atgtgaagca gagtaagcaa aaacagtctc
      181 gtcgactttt gtttcgtttg tttttattaa tatgtatcgt cgtagggtta ctgatcgctt
      241 tatatccatt ttatgtggat tctttaaata gttttattga tcaaaaacgt atcgaacaag
      301 tacaaaaacg gaccgcagct gaaattgaag tgcagcgaaa gaaaatggag gagaaaaacc
      361 aacgattaac tgaccaagga ttcaatcctg gtactgatcc tttcaatgaa aagaacagaa
      421 atgaaagtac aacatccagc caattagagg aatggctgat cggaagtatc aatataccta
      481 agatccaatt aaatatttca ctttatgatc ggctcaatgg taggatcctt gaaaatggag
      541 caggcgtctt gcaaggaacc tctttcccgc ttggtggaaa ttcgacgcat tcagtgatct
      601 ctgctcacag cggtttacct aatcgacggt tatttaccga attagatcgt ttagaaaacg
      661 gagacacgtt tattttgact gttttggggg agaaattggc ttatcaagtt aaaaatatac
      721 aagtggtact gccagatgac acgtctgttc taacgatttc agaaggagaa gacttagtta
      781 ccttgcttac atgtacgccg tatatgatca acacgcatcg tttgctagtg acaggacatc
      841 ggattcctta taacgaatcg gtaaaaaaag aagaaggaaa aggaaatcag gaaagaacca
      901 tgcggcaatg gtttattctt gcaggaacga taatagctgt agtcatactg ttagtgttta
      961 tcggacgact aatctatcag tatagattaa gtaaaaaagt gcttgatttt tcatttatta
     1021 ttagtgattc gtcagggaaa ccggtaaatg gtagttcatt cattttgaaa cataagaaaa
     1081 aaacacttac tagaaatggt gtgccgtttt cagtacaaag cgaccatcat gggaaagtca
     1141 agctggatca attgcctggt ggaacttatc gaatcgtttc agctgatgat cctaaagttg
     1201 ctgtttcttt tggtattaga aaagtaaaac aagagaagat atactttttt gaggggcgaa
     1261 aacttgtaaa agagcttcaa aaaaatggtt tttattttaa aatcaatgaa taagtaagga
     1321 gagaaaaaat gatccgcaca acaacaaaat tgtctgccaa agaactggaa tctgttctat
     1381 cgatttggct gcatgctaac ttggattctc atgaattcat tccatcaact tattgggaga
     1441 atcaagtaga atctgttagg aaagctttac cagatgcagt gatttactta gcagaaatag
     1501 atcaagaaat tattggtttt gctggggtga ttgatcagtt tatcgcagga atctttgtga
     1561 aaagggaata ccggcaacaa agaatcggta cgcaactaat gaaaaaaata aaagaaacca
     1621 ataaaacact gactttgaca gtctatcaaa aaaatcaatc agctattcat ttctatcttt
     1681 cacaaggttt tacaattcaa acacagcaaa aagatgaagc agtaaatgag gaggaacttg
     1741 tgatgaaatg gaatcaaaac gctgattttt gatgaacaga ataacgaaat aatgctccca
     1801 gcctaatttt ttgctgggag cattatttca ttttgaagtc actaatttaa ctgtgtgaat
     1861 atgcagttgt tacaatc
//
LOCUS       DACEUZ010000207         1859 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.208, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000207 DACEUZ010000000
VERSION     DACEUZ010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1859
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.208"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            416..1804
                     /gene="ltrA"
                     /locus_tag="IXG44_002571"
     CDS             416..1804
                     /gene="ltrA"
                     /locus_tag="IXG44_002571"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAQ1659262.1"
                     /translation="MRRPQKTRKLGYHQRASVEHEGYDEVRSTSCGEQRKQDGVENLF
                     GRILDRNNLNQAYLQVVRNKGAAGADGMTVDQLLPYLKEHREELLSNLRQGNYKPQPV
                     LRVEIPKPNGGVRKLGIPTVIDRMLQQAINQVLQPLFEPEFSDNSYGFRPNRSAHDAV
                     KKAKSYYEQGYRYVVDIDMKAYFDTVNHDKLMYFIERKVKDKQVLKLIRQYLMSGVME
                     NGLITTTEEGTPQGGNLSPLLSNIYLNEFDQVLENLGHKFVRYADDCNIYVRSKRAGQ
                     RVMKKSIQFLEEQLKLTVNREKSAVGSPLKRKFLGFCILPTKKGIKIRPHAKAKQRVK
                     SKLKQLTKRNRGRSIQLILKEIKQLMTGWINYYGIGEMKEFMRELNGWLKRRIRQYIW
                     KQWKNPRTKRKNLIRLGIDKAKAYEWSNTRKGIWSVSKSHILHRSLTDKELARQGYED
                     ISLKYQFVHSTY"
BASE COUNT          654 a          343 c          444 g          418 t
ORIGIN      
        1 aacttggtgg tgaaagtcca ctacaggctt ggcagtagga actgttagcg aaagacaagg
       61 cggcaatcgt gaggttgtgg ctgaaggaag tcggacgcaa actcctgaac tgacgaacag
      121 aaactagata caaggctgaa ttaggtcgga taagtcttca tcacaagaca aagtccaata
      181 ctgcccgaaa cctatacagt aaatctagca gttacatggg aggaaagttt tcactcttac
      241 cgggggagat ctcatgaggg tgttgtgggc gttgaatgaa aacaaaccag cacaacgatc
      301 atggcgacat gatggtgaat catgagaagt cagccgaggt catagtagta acgacactga
      361 ccgttacgaa ggactgaaca ataagaatct tgaataattt aggaggtgtg agaagatgcg
      421 acgaccgcag aaaacaagaa aacttggcta tcatcaaaga gctagtgtgg aacacgaagg
      481 gtatgatgaa gtgcgcagca cttcttgcgg tgaacagagg aaacaagatg gtgtagaaaa
      541 tctgtttggg agaattctag atcggaataa tctcaatcaa gcgtatcttc aagtagtcag
      601 aaataaaggg gcggctggtg cagatgggat gaccgtcgat caactgcttc cttacttgaa
      661 agagcaccgc gaggaattac tatccaattt acgccaaggg aactataaac ctcagcctgt
      721 tttgcgagta gaaataccga aaccaaacgg cggtgtaaga aaactaggaa ttcccacggt
      781 tattgatcgt atgcttcaac aggcaatcaa tcaagtgtta caaccgctat ttgaaccaga
      841 attctcagat aatagttatg ggtttcgccc aaatcgcagc gcacatgacg cagtgaagaa
      901 agcaaaatct tattatgaac aaggttaccg atatgtggtg gacattgata tgaaagccta
      961 ctttgacacc gtaaatcacg ataagctgat gtatttcatt gagcggaaag taaaggacaa
     1021 acaagtccta aaactgatcc gtcaatactt aatgagtggt gtaatggaaa atggactcat
     1081 cacaacaact gaagaaggaa cgccccaagg aggcaatctc tctcccctgt taagtaacat
     1141 ctacctgaac gaatttgatc aagtgctaga aaatctagga cataaatttg ttcgatacgc
     1201 cgatgactgc aatatctatg ttagaagcaa acgagcagga cagcgtgtga tgaagaaatc
     1261 cattcaattt ctcgaagaac aactaaagtt gacggtaaat cgagaaaaga gtgcggtagg
     1321 aagtccattg aaacgaaagt ttcttggatt ctgcattctg ccaacaaaga aaggtattaa
     1381 aattcgccct cacgcaaaag caaaacagcg tgtgaaaagc aagctcaaac aactcactaa
     1441 aagaaaccgt ggaagaagta tccaacttat tttaaaagaa atcaagcaac taatgactgg
     1501 ttggatcaac tactatggaa ttggtgagat gaaagaattc atgagggagt taaacggttg
     1561 gttgaagcga agaatcagac aatatatttg gaaacaatgg aagaatccaa gaaccaaaag
     1621 aaaaaatctg attcgtttag ggatcgataa agcgaaagcg tatgaatggt caaatacaag
     1681 aaaaggtatt tggtcagtct ccaaaagtca tattcttcat cgatcattaa cagacaaaga
     1741 actggcccgc caaggatatg aggacatctc cttgaagtac cagttcgtac actcaactta
     1801 ttgaaccgcc gtatacgggt ccgtacgtac ggtggtgtga gaggggcgga aggtcagtt
//
LOCUS       DACEUZ010000208         1852 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.210, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000208 DACEUZ010000000
VERSION     DACEUZ010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1852
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.210"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(493..1785)
                     /locus_tag="IXG44_002572"
                     /pseudo
     CDS             complement(493..1785)
                     /locus_tag="IXG44_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
BASE COUNT          577 a          371 c          310 g          594 t
ORIGIN      
        1 ttatattata aattcacatg attatcgaat tttattaagt tagtttttct tgtaaatctc
       61 tgttcccaat gaaactcgag acacttattt taaagaggaa acaatgtggt atttaaatag
      121 ccctcttgta tgggctactc tgtgatactt attagccatt aaactatttt acccacatag
      181 catatatttt gaaatcgttc attccgtaaa ttcaactaaa tatgcctaaa aaaataaaaa
      241 atctaagata gaataagatt tattcagtct tagatggatt tatagcaact ttttcgtaac
      301 aactttaaaa aatacgaaaa gaaagagctg ttttactctg actacgttgc taaattgtcg
      361 tttaatcatt catttaaacc aaatcttttt ttatagaatt cgttttaatc ctcctgaatc
      421 caaaaactat gaaaaccgta attaaatagt taagagtttt taaccaccat aattctctcg
      481 gctattttga aatcagataa attttgatcg gacacatagt tgaataaacc taccgttgtt
      541 acggaaggta aatcgcatac ttttcattct agaggagaag gtctcgttga tcattaactg
      601 attaagcacc atagcccagt tttgaattcg accacctgac cacttcgcgt gtaattcttt
      661 cgtgcgtaaa taaagtagtt ttaggagtgc attctcatta gagaacgctc ctttttttgt
      721 gacttttctg aagctggagt ggacactttc aaccgcattg gtagtgtaca taatttttcg
      781 aatggcacta ccataatcaa atagttgttc aacatgtgca aagttccgtt tccagacatc
      841 tacagcacca gaataatgag accaccgatt ttgaaaactg tcaaaagcag catgtgcagc
      901 gtttagagaa gaagcaccgt agaacttttt catatctcgg cagacttcct tatagtcctt
      961 acttggaata tagcgcaatg catttcgaac aagatgaaca atacagcgct gaacaattac
     1021 cgatggaaag atcgcttttg cgccttcttc gaggccagaa acaccgtcca tcgaaatgaa
     1081 aaagacatct tcgacaccac gtgctttcag ttcatcaaat acttgcatcc agcgattttt
     1141 agattctgtt tgatttaacc ataatcctaa aatctcctta tttcctttga gatcatagcc
     1201 aagaatggtg tgtacagcat attctttggc ttcataattt tctcgtaaag taacatacat
     1261 acaatcaacg aatagaaagg cataacactt tgctaggggg cgggcttgcc attcttccaa
     1321 ttcaggaagg acagcgtcag tgatatctga aatcatttca tgggaaatat caaagccata
     1381 aatagcttcg acggttgcgg caatatctca ttgactcatt cctcgtgcat acatggaaag
     1441 aaccttccct tcgatgtcgg agacatctcg ttttctctta ggaattaact ctggttcaaa
     1501 ggaagcttcc cggtctctag gaacatcaat agctacttca ccaaaactgg ttttaagcgt
     1561 tttagttcca tagccatttc gacggttatc gtgttcctta ggctctttag aatgggcatc
     1621 ataacctaaa tgattattca attctccttg aagcattttt tcaaaaaggg gcccaaacac
     1681 atctttcaaa gcatcttgca tgtcatcgac agattcaggt tgataggcat tcagaatgga
     1741 ttcagctaac ttttcggcat caggatttct tttctttcta gccatcgtgt aatcacctct
     1801 tgatcttatt gtagaaaaag aaaaaccgcg aagcaaccac ctgcctagga tt
//
LOCUS       DACEUZ010000209         1812 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.212, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000209 DACEUZ010000000
VERSION     DACEUZ010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1812
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.212"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(128..604)
                     /locus_tag="IXG44_002573"
     CDS             complement(128..604)
                     /locus_tag="IXG44_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAQ1659263.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(617..>1812)
                     /locus_tag="IXG44_002574"
     CDS             complement(617..>1812)
                     /locus_tag="IXG44_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1659264.1"
                     /translation="GFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLG
                     FFGGSRFIPIVSSLAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRL
                     TGAVGLHHTIYPLFWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFA
                     GRFATMMFGLPAACYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAP
                     WLYVIHAFLDGLSFYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIA
                     WAFIYFFVFTFFIKKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENI
                     ESVEACATRLRVAVTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQI
                     LGKDN"
BASE COUNT          652 a          343 c          255 g          562 t
ORIGIN      
        1 ttggcatggg gaatcattac taataaaata aaaaaatgaa cagaaagaaa acagttcttt
       61 ctgttcacca acttcatttg acaaatatcc tatttagctt attttatagc tttttatata
      121 cactttttta aaaagtgatt tttccacatg aattcccatg agcaattcga ccattatcta
      181 ttattaaatc attgaactga tttttctccg gaaaaacaac tataacagaa gtatctttac
      241 cagattttat tattttgtca aaatccacat caaccaacga ggtttcagga gtaacctcat
      301 cattttcttg aacacaaatc gaaaaaggct ctccttgtaa ttcaactgta tcaattccaa
      361 tatgaatcaa ataatcaatg cctgagttgc tttttatatt aatagcatgt ttagtaggaa
      421 atatatttgt tactcgacca gccaatggag cataaatttt tgaaccatca ggtatgacgg
      481 caaaaccatc acctagtaat ttttgtgaaa agacaggatc atttacttct gtgatggaaa
      541 tgatttttcc ggaacaaggt gcataaaaag tttcttgttt tttctttttg aaaatttcaa
      601 acatattaaa aacctcctaa ttatcttttc ctaatatttg attgatttct tgacttaata
      661 agtcagcttt ccctccaaaa acagcttgga tgccaccctt cacttcgaag acagcagttg
      721 caccagtgtc ttttattgtt tgtttttgaa taatctcccc attcgtaaca gcaacacgca
      781 aacgagttgc acaagcttca acagactcaa tattttcttt tccaccaaga gcttcaataa
      841 tgataagaga ctcttcatgt aatgatgtat ttttgtttat tactttttca gaatgaactt
      901 ctgattcttc ttccattcca ggaatagcta ccttaaattt tttgataaag aatgtaaaaa
      961 caaagaaata tataaatgcc catgcaatac caaaaggaat taattttaac caattagttt
     1021 tgtcattccc ttgaagtaca ccgaataata aaaagtcgat caaaccacct gaaaaggaat
     1081 tccctacacg aatatttaag atatcagcaa aataaaaaga taatccatct aagaatgcat
     1141 gaatcacata aagccaagga gcaacaaata aaaacatata ttcaactggt tcagtaattc
     1201 cagttaaaaa tgatgtaagg gccccgctaa aataaagccc accattcttt ttacgatttt
     1261 tcttaggaat tgcacgatac attgcataac atgctgctgg taatccaaac atcattgttg
     1321 caaatcttcc tgcaaaaaat ctagtaccat aagtaaacag accagtgtgg tttggatcag
     1381 ctagttgggc aaagaaaata ttttgggctc cagcaattgt tgatccagct acagtttcgg
     1441 taccacctaa agatgtatac cagaacaaag ggtaaatggt gtggtgtagt ccaacagcac
     1501 ctgttaaacg caataaaaaa ccatataaaa aagttcctaa actacccatt tgggcaattt
     1561 gttcaccagc aactactaaa ccattttgaa ttggtggcca aattaaataa aagaaagaac
     1621 caataatgat tgcagctaaa ctagaaacaa tcggaataaa tcttgatcct ccaaaaaaac
     1681 ctaagaattg tggtaattca atttttctat aacgattgtg taaaaatgca acagtaccac
     1741 caacaacaat tgatcccact acacctgtat ctagtgttgc atcttttgct gaaaaaagtt
     1801 caagaaaccc ac
//
LOCUS       DACEUZ010000210         1778 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.213, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000210 DACEUZ010000000
VERSION     DACEUZ010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1778
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.213"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            1..72
                     /locus_tag="IXG44_002575"
     CDS             1..72
                     /locus_tag="IXG44_002575"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAQ1659265.1"
                     /translation="MCSLLVGLLSALFSYWLNNRNKK"
     gene            complement(374..619)
                     /locus_tag="IXG44_002576"
     CDS             complement(374..619)
                     /locus_tag="IXG44_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1659266.1"
                     /translation="MRLEKEKMEKFPYEYFQNLEKLEQAIQAIKFDLTDEKLLEYKNM
                     TPERKRSLEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
     gene            965..1249
                     /locus_tag="IXG44_002577"
     CDS             965..1249
                     /locus_tag="IXG44_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1659267.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            1252..1668
                     /locus_tag="IXG44_002578"
     CDS             1252..1668
                     /locus_tag="IXG44_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1659268.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
BASE COUNT          614 a          324 c          267 g          573 t
ORIGIN      
        1 atttgctccc ttcttgttgg gcttttaagc gctttgttca gttactggtt gaataaccga
       61 aacaagaagt aaaacttaat taaggcgact cagcccacaa aaaaagcccc agagaatccg
      121 acctctctgg ggctttcgtg tgtttcgtgt tcacttattt gcaatcaaat tataacagaa
      181 ataaatattt atttcaaaaa tatcctacaa aagtctaaaa tccgtaatta tttcttatat
      241 gcaacattta caaataaagt cgctctgaga gctcgtattt ttcgatttaa gacgttttat
      301 ttagtttttg gtgtatttca ttggtccaag ctaaaaaact cttagaagcg attttagagc
      361 catttaaacc cacttaatta tattctccat gaaattcgct ccatgcgtca aaagctgcgt
      421 ctgaaattcc gccgtcttcg ttcactagtt gcattaattt ttccaacgat cgttttcttt
      481 cgggtgtcat atttttatat tccaacaatt tttcgtctgt taaatcgaac ttgattgctt
      541 gaattgcttg ctctaatttt tctaagtttt gaaaatattc gtaaggaaat ttttccattt
      601 tttctttctc cagtctcaca atttttctag aaataaaatc acttctttgc cgtctatact
      661 ctcaaaaaaa atgtcgtttg aaaaactaaa taaatttttg atcgtgcatt tttaaatttg
      721 gttatcgatt acacgtgcag cactcccccc ccgaatttta aagagcataa ggaaacgtgt
      781 acccactttc taaaaaaagt gggtagatag gctgtgccta tcacgtttct cagaaaaaaa
      841 tttgatatca ctttttcgcc atcgtgggta gcttatgtat agcattttgg caaaaaacag
      901 ctatacaaat atagtatttg tatggtatac ataaattata cataattgca aaaggagatt
      961 ttttatggac attttagttc ggaatattga tccaaagttt gttgcaacaa tagacaaacg
     1021 ttgccaagaa ctcacgaata aaacaggaga aaaatggagt cgaaataaat atctaaaagt
     1081 tttaatcgaa agtgattttg accgtcaact tacgacttac aaaaaagacc aattcgattt
     1141 attgctcgat aaatttattg ctatccaaac agaaaatact caaatattaa gcgagtatgt
     1201 ctacacaaat aatcaaatca ttgaaatgct tatcggagga atgatataac catgagaaaa
     1261 acttctgtcc aaaaacatgt tactttgtac cctagtcatg ttgacgtttt agattccatc
     1321 atcgaaaaaa atccagatat caataactac tcggaagcca ttcgcttttt atgtatgagt
     1381 tatgaacccg ataccaacac gaccactacc agcaatcagg caaaactaaa tgctatggga
     1441 aaagaaattt ccatcatgac tgaaatcatc gcagaatttg ctgatttata tctaaaagat
     1501 acaggcgttc ttattggcac aaattctaaa gtatatcaag aagtgaaaga aaaaatcgaa
     1561 gctaaactaa atccatcaaa tcgaaatcga ccaaagaatc atcaaaaaga aaccagtcaa
     1621 acagtcactt ctagaaattt cgacccaaca aattttctaa aacgataaaa aacagatagg
     1681 agttattcct atctgttttt tatttagaag ccccatacaa tatgctagaa tgtactagca
     1741 atcttagtcg gttgtaaggg acttgtctac ttgcttaa
//
LOCUS       DACEUZ010000211         1774 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.214, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000211 DACEUZ010000000
VERSION     DACEUZ010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1774
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.214"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            23..646
                     /locus_tag="IXG44_002579"
     CDS             23..646
                     /locus_tag="IXG44_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1659269.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1096..>1774
                     /locus_tag="IXG44_002580"
     CDS             1096..>1774
                     /locus_tag="IXG44_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1659270.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQ"
BASE COUNT          626 a          284 c          376 g          488 t
ORIGIN      
        1 aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat cgagagtaca
       61 agaaatgaat gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt attttcgacg
      121 tcttgttttt ctgctcatct tatcattgct gttaacagtt gcaatgtttt tattgccgga
      181 aaaaatccga gaagagcaag taaatagtct cgtttattcg tccataccaa ccagcaaatt
      241 gaaacctttt gattacagca aagctgatca gaaaatcaaa caaagccggg cgatatcggt
      301 gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt ttaatgatcc
      361 tgagcttatg gaagaattga atcgaccgat cttttattat ccgatcgttt atgatgtaca
      421 tgaactggaa caaaaatata acatacgaac ggatgaagtg acatttattt tctttgatgg
      481 aggaaaagaa gcaaatcgga taactgcggg taagggagga ataactgatt ttgataagga
      541 gttgatccct gaactgaatc gacttccgtt agccaatatc aaacaattag aagaagaatt
      601 ggatagaacg ggagtaactt cccaaaattc cgagattgct aattgagaca ttggcgcgat
      661 gaaacggtgg gtctatatag cgctattttt aattatcaga acaagtgatg tttgatcttc
      721 aaaataacaa ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt cgattagact
      781 taaatcaatt cttaaggaaa gggtgacctg taatgaactt atcaaaacgt atttcagcag
      841 aaaacaaaaa aaattggcgc agctggcgta gataagttgt attaatggtg agactttccc
      901 atagatacaa ctttttagag tgcatttcta aagcttgtat ctatgctaga catgaacatt
      961 gactgccgca tagattagaa tctatgcggt tttttgtgta aagacaatga ccgctttggc
     1021 cattgtcttt ttttgtagac tggatacttg cctgattata ataaataaaa agattgactt
     1081 tgcaggagga aaaaaatgaa aaacaaacga ttaataacag ttgtagtaat tattttagtt
     1141 tatttagcag gtgcatttat ttatggaaaa gcctcaggcg aaaaagaaag tacttcgacg
     1201 aaccagacaa aaaaaacaac agttgttggg gtattgcaat acgtgagcca tccggcatta
     1261 gatgagattt atcgaggaat caaagatggt ctgaaagaag aaggattaaa agaaggagaa
     1321 aatcttgaga tcaaatttca aaatggacaa gccgatcaga gcaagctagc cacaatgagt
     1381 caacagctag tacaatctga tccagatgcc ttgattggaa tcgcaacacc tgccgctcaa
     1441 tcattagcaa acacgacttc atcgatccca gttgtgttag gtgctgtaac ggatcctgtg
     1501 ggggctggat tagtcgcttc tttagagaaa cctggtggta atgtaacagg tgtttctgat
     1561 cagccgcctg ttacttcaca aatcaagctt gcttctgatt tgttgccaaa tgcaaaaaaa
     1621 gtcggaatct tgtatgcttc ttcagaggat aattcaaaat accaagtagc acaagcagaa
     1681 gaagctgcaa aaaaagaagg gttagaatca gtaaaatatg cggtgccttc cactaatgag
     1741 attgcacaaa cggttcaagt gatgagccga caag
//
LOCUS       DACEUZ010000212         1763 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.215, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000212 DACEUZ010000000
VERSION     DACEUZ010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1763
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.215"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(430..1002)
                     /locus_tag="IXG44_002581"
     CDS             complement(430..1002)
                     /locus_tag="IXG44_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1659271.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(1018..1623)
                     /locus_tag="IXG44_002582"
     CDS             complement(1018..1623)
                     /locus_tag="IXG44_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HAQ1659272.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT          516 a          360 c          259 g          628 t
ORIGIN      
        1 taatatcaca ataggaatgc tgtttattat attagcagca gtgctgattt ttatgatagt
       61 tatttggttc ttaaatgaaa gatctaaaga caatcaacac cgtagggata tggagataaa
      121 atcaatagag tcaatagatt atttatcgat aataacacta aaaaagccac gttcctaaac
      181 gtgacttacg ctgaacgata tgtgttgttt ggtagacgaa caaatcgttc attataaata
      241 tatgatggta tcctaatata gtaagcgtgg ctagcatacc tttaattact ttacctttta
      301 cagttaccat acacattttt tgagcagaaa tcaagtaaat aaaaaaatat tatatttcac
      361 aacatgctaa tagactcata tctagaacgc attaagtatt tacctgtctg gttcgctgag
      421 acttcttttt taactttctt caattttctt ctttattcta aaaagcgtac tgcgactgaa
      481 accggttata gcttcaactt ccttatacga tttacctgaa gtaagcagtt catatgcgtg
      541 cctcttcttc ggtgtgattg ttgctttcgg acgtccttct ctgaaattag gattattctt
      601 tcttgcaaac atttttcctt cttgggtacg ttccacaatc atatcacgtt ccatttctgc
      661 tacactcaac aacgtccgtg tcaccattcg ccccatggac gtattttcaa tcgttcctag
      721 attcaaaacc ttgatcgtta cgttatcatc gagtaaaggt tcaatgatct ctaatgcttc
      781 tctcgtgtta cgagcaaatc gatcaagctt cgtaacaact atttcatctc caggcttcag
      841 accagccaac agctgattga attttggccg atcccgtttt gtacccgtaa atttttctga
      901 aaaaatattc ttcttctcta cgccgttctc aagcaactgt tgaatttgtt cttttgtact
      961 ttgagaagcg gttgagacac gtgcatatcc atatttcgtc atttggtcca cctactttta
     1021 caaatcatct atcgtatact cagaataccc ttcatagaaa tagctgacat cgattccttt
     1081 catatataac tcgcgatcgt caattttatc agtcagagca ttggaaatta gatagcgtat
     1141 ttccaaatcg tttatgggac ttcgctccat tgcagaaaga tagtctgctt tatcgattaa
     1201 gttccaatct acaacttttt gcaattcttc ctttaaaatg agatccagcc aaattctagt
     1261 acttcggcca tttccttctc gaaatgggtg agcgatattc atctccacat atttcttgat
     1321 aatctcttca aaagaagttt gtggcatctg atcaatatgc tgtaacgaat gttctaaata
     1381 cataactggt gcaaatcgaa aatttccttt tgcaatattg accgatcgga tttttccggc
     1441 aaaatcgtaa atatccgaaa atagaaattc atgaatttca gataaacctt taaatgtacc
     1501 aacttcgatt tcatttattt ttccacaatc gtacaattct ttcgcttttt gtttactgag
     1561 tttttcttca accttagcca actcaacttg attggttaat cccaatttat tctctaaaat
     1621 catgcgaaat ctccttaact ttatgacata agtttatgaa acactctcaa ccccattata
     1681 tcgcgatttt gtttatgtgt caaaaactat taagtttctg accctcgata gatttttcaa
     1741 gtgtctctat tttgcacagt cta
//
LOCUS       DACEUZ010000213         1745 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.216, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000213 DACEUZ010000000
VERSION     DACEUZ010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1745
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.216"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            378..1628
                     /locus_tag="IXG44_002583"
     CDS             378..1628
                     /locus_tag="IXG44_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAQ1659273.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     ETILEFNNSYHFDQIVIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFS
                     RMFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQH
                     FLENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGK
                     GRFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQ
                     VIVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQ
                     SQNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARK
                     FTRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          578 a          334 c          307 g          526 t
ORIGIN      
        1 taatagtttt tattcaaaat gtttctagat tataaaattt attgaataac acattcctaa
       61 cggaatttgt ttcgatattg tgtgatttct cagagtacaa tggatgtatg gaaaggatcc
      121 aacgggaatt tgctatttcc tcctgtagcg tttgtctacc ctgtttagtc tgttacccga
      181 accactgtta tcactaccaa ctcattagct gtttcgataa tggactagac tttcaaatag
      241 tagtttggaa ggcagcttct agctctttta tcaccgtatt ctattaggcg aggttgtggc
      301 tatacagtga gtttctggga aatcccacag tatcgaagca ttttccaaaa tttatatcga
      361 aaggaggtcc ttcttccatg aaattatttg tcggtattga cgttagctct gaaaaactag
      421 atgtttgttt tttaacagac gacaatcaat tgtctatctt atctgaaatc tctgttgcca
      481 atgacatcga aggtgcttca ttcatacgag aaacaattct tgaattcaat aacagctacc
      541 actttgatca aattgtgatt ggcatggagt caacgtccat gtacagcttc catccttcca
      601 tgttttttca tgaagatgag aaactaaaga agctcaacac acttgtaacg attgaaaatc
      661 cttttcgggt gaaacaattt agtcgcatgt ttgatgagaa taaaactgat cgaaacgatg
      721 cattaagaat cgctgatttc ttgcgtattc aacgattcac aacttcccct atcaaagaag
      781 agaaatacat ggccttacag cgtttaacac gtactcgcta tcaacttatt aagcaattga
      841 ttcgtacaaa gcaacatttt cttgaaaatc taacgtacaa atgtaatacg cttgctcgag
      901 aaatgcgaga cgaatcgacg agtcttttta gtgcaaccct catttcactc atgacggaag
      961 atttcacatt ggatgaactt gctgagcttc ctttagaagc tttctgtgat ttacttcaag
     1021 aaaaaggaaa aggccgtttc aagcaaccag aaaaaatcgc taaagcaatt caacgtgcaa
     1081 ttaccatgag ttaccgccta ggtgctcttg cacaagaatc aattaatgtt gttttaagtg
     1141 ttctcgtacg agaaatacga gcacttgaaa aaaatatcaa agaattagat aaagccatcg
     1201 aacaagtaat tgttgtcatt ccagaatatc aatgcttaac cagtattcca ggcgttggta
     1261 aagtctatgc tgccggttta atcgctgaaa ttggtcaaat cgaaagattt gaagatcaga
     1321 caaaattggc aaaatacgct ggattaagtt ggaaagtgaa ccaatctgga aactatcagt
     1381 cgcaaaatac acctctgaca aaacaaggaa atcgatattt tagatactac ttagttgaag
     1441 ctgccaactc tgtaaaaaat tatcttcctg aatacaaagc tttttatcaa agtaaatata
     1501 aggaggttcc aaagcatcaa cacaaacgtg cactcgtcct aaccgcaaga aaatttacgc
     1561 gtctggtgga tacgctacta cgtaaacacc aactctatac gccaccaagg agtgtgatag
     1621 agaaataaca atccgttatt gacgcaaatc cttgaatcaa cccgaaaaaa atttgatttt
     1681 gatcgggtct agtttcgtgc gcttcaaaac atattactca ataaagaaaa tcttaaattt
     1741 catct
//
LOCUS       DACEUZ010000214         1691 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.217, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000214 DACEUZ010000000
VERSION     DACEUZ010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1691
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.217"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            107..502
                     /locus_tag="IXG44_002584"
     CDS             107..502
                     /locus_tag="IXG44_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1659274.1"
                     /translation="MKKKQTKLSLADILTQLTATEDGVVEFERSEISVVDDRYFKMPY
                     FFDQAKVICLCGYDGVRDYFGIRITEEKVVWVNNHTELGALAFEGTVLDNISIVFEEE
                     SFTLECDKLTRYIDPKFYEDKNLAWELAL"
     gene            484..750
                     /locus_tag="IXG44_002585"
     CDS             484..750
                     /locus_tag="IXG44_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1659275.1"
                     /translation="MGTGFIDRVLVQVKEERKITMKYNLLDHKRMWVEKLEEDLEQLE
                     LLGHNKTSTVYKEGLKRTKKAQKELATLESRVQKKEVDLTHTYL"
     gene            760..993
                     /locus_tag="IXG44_002586"
     CDS             760..993
                     /locus_tag="IXG44_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2786 domain-containing protein"
                     /protein_id="HAQ1659276.1"
                     /translation="MADKRKEKIQKLLALAADVNDEESVSALAKAQQLMMDYDLTEDE
                     IFNYHEQQRIEAVETHVIYRGRPQKWLYRLFRN"
     gene            1336..1518
                     /locus_tag="IXG44_002587"
     CDS             1336..1518
                     /locus_tag="IXG44_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterocin"
                     /protein_id="HAQ1659277.1"
                     /translation="MSVSTLGITVDAATYYGNGVYCNTQKCWVDWSRARSEIVDRGVK
                     AYVNGFTKVLGGVGGR"
     gene            1508..>1691
                     /locus_tag="IXG44_002588"
     CDS             1508..>1691
                     /locus_tag="IXG44_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1659278.1"
                     /translation="MEDKKEKLFDLIANAYNSKSYNNVEVKEGFLQDAKKIREDKPYP
                     EIIISVYKHLSKLKAEE"
BASE COUNT          629 a          207 c          353 g          502 t
ORIGIN      
        1 agaaaaagca ggatttgagg taccagcact aagaaagtga cgtcactttt tatactaaaa
       61 cctaaaagtg atatcacttt tagacaatgg aaaaggtgga aataagatga agaaaaaaca
      121 aactaaacta tcgttagcag atattttaac acagttaacc gcaactgaag atggtgttgt
      181 agaatttgaa cgatctgaaa tttcggtagt agatgatcgt tactttaaaa tgccttactt
      241 ttttgaccaa gcaaaggtca tttgcctttg tggctacgat ggggtacgtg attactttgg
      301 aattagaatt actgaagaaa aagtcgtttg ggtaaacaat catacagaat taggtgcgct
      361 agcctttgaa ggaactgtat tggacaatat ctctattgta ttcgaggaag aaagctttac
      421 gcttgaatgc gataagttaa cccgatatat cgatccaaaa ttctatgagg ataaaaacct
      481 ggcatgggaa ctggctttat agacagagtg ctagtgcaag taaaggaaga aaggaagatc
      541 accatgaaat acaatttatt agaccataag cgaatgtggg tagagaaatt agaggaagac
      601 ttagaacagt tggaattgtt aggtcataat aagacttcaa cggtgtataa agaaggacta
      661 aaacgaacga aaaaagcaca aaaagaacta gcaacactgg aaagtagggt tcagaaaaaa
      721 gaagtagact taacacacac ttacctttag gagtagaaaa tggcagacaa gagaaaagaa
      781 aagattcaaa aattactggc gcttgcggcg gatgtaaatg atgaggaatc agtgagcgca
      841 ttagccaaag cccagcaatt gatgatggac tatgatctta ctgaagatga gattttcaat
      901 tatcatgagc agcaacggat agaagcagta gaaacgcacg tgatctatcg tggacgtccg
      961 caaaaatggc tgtatcgttt atttagaaat taagataaaa aaggctagtt aacaggtttg
     1021 ttgactggcc ttttttgata tgtttgtaca tgttttattg tgttttaatt tttctctttt
     1081 ttatagcaag ctccttttat aataaatatt ccgaaaagtg aagaccattt tttatttatt
     1141 aagactatta ttattagtga gataaaattt gattgatctt acagaatatt ggtgacgatt
     1201 atgacactaa agatactatt tatgaaaatt ttgcaaaaat gttctgaaaa ttacatataa
     1261 ttgttttata aaaaagggag gcaattatat gaagaaaaaa tttgttagta ttttttgatt
     1321 ttaggaattg ttttattgag tgtatctact ttaggaatta cagtagatgc tgcaacttat
     1381 tatggaaatg gtgtttattg taatactcaa aaatgttggg tagattggag tagagctcgc
     1441 tctgagattg ttgatagagg agtaaaagct tacgttaatg gttttacaaa agtgctaggt
     1501 ggagtcggtg gaagataaaa aagaaaaact ttttgactta attgcgaatg catataattc
     1561 aaagtcatat aataatgtag aagtaaaaga aggatttctt caagatgcta agaaaatacg
     1621 agaggataaa ccatatcctg aaattattat atcagtgtac aaacatttat ctaaattaaa
     1681 agctgaggaa c
//
LOCUS       DACEUZ010000215         1664 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.218, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000215 DACEUZ010000000
VERSION     DACEUZ010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.218"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            131..505
                     /locus_tag="IXG44_002589"
     CDS             131..505
                     /locus_tag="IXG44_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAQ1659279.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="IXG44_002590"
     CDS             complement(619..1248)
                     /locus_tag="IXG44_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ1659280.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLPLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="IXG44_002591"
     tRNA            complement(1382..1454)
                     /locus_tag="IXG44_002591"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
BASE COUNT          584 a          240 c          284 g          556 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt aggggaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt actt
//
LOCUS       DACEUZ010000216         1633 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.219, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000216 DACEUZ010000000
VERSION     DACEUZ010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1633
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.219"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            146..1630
                     /gene="guaB"
                     /locus_tag="IXG44_002592"
     CDS             146..1630
                     /gene="guaB"
                     /locus_tag="IXG44_002592"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HAQ1659281.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
BASE COUNT          562 a          284 c          378 g          409 t
ORIGIN      
        1 acaatctctt ttaatacaca cagatgtaaa cgctttatat tggtattcta tgagaaagct
       61 aacttgattc caagtagatc attgacttaa atgaaaaaat caattatttt aatagataaa
      121 ctaaattaga ggagtggtac taaaaatgtc caactgggaa acaaagttca cgaaaaaagg
      181 atttactttt gatgatgtct tattgatacc agcagaaagt cacgtgttac caaacgaagt
      241 ggatatgagc gtacaattag ctaaaaatat taagttgaat attccaatca tcagtgcaag
      301 tatggatact gttaccgaca gcaagatggc cattgctatg gcaagacaag gcggactagg
      361 tgtgatccat aaaaacatga cgatcagcca gcaagcggat gaagtaagaa aagtcaaacg
      421 ttctgaaagt ggcgtaatca ttgatccgtt cttcttaacg ccacagcatc ttgtagcaga
      481 cgcagaagaa ttgatgagca aatatcgtat cagtggtgtg ccaatcgttg aaacacttga
      541 aaacagaaaa ttggtaggga taattacaaa ccgcgacatg cgttttgtaa cagattatca
      601 catgccgatt gctgatgtga tgacaaaaga caacttagtg actgccccag taggtacctc
      661 tctaaaagat gcagaaaaga ttttgcaaaa acataaaatc gaaaaattac caatcgtaga
      721 taacgaaggt cgtttaagcg gattgatcac gatcaaagac atcgagaaag tgatcgaatt
      781 tccaaatgca gcaaaagatg aacatggtcg attgcttgta gcagcagccg ttggtgtaac
      841 aagcgataca tttgagcgtg cgaatgcttt attagaagca ggggcagatg caatcatcat
      901 cgatacagct catggacata gtgcaggtgt gatccgaaaa attcaagaaa tccgttcaac
      961 ttttgcagat gcaacattga ttgcaggaaa cgttgcgacg gctgaagcga caaaagcttt
     1021 atatgatgca ggtgtagatg tcgtaaaagt tggtatcgga ccaggatcga tttgtacgac
     1081 acgtgtggta gccggtgtag gtgtacctca gctcacagct atttatgatg cagcatcagt
     1141 tgctcgtcaa tatggcaaag cgattattgc agacggaggg atcaaatatt ctggtgacat
     1201 cgtcaaagca ttagctgccg gcggacatgc agtgatgcta ggaagtatgt tggcagggac
     1261 agatgaatca ccaggtgaat tcgaaatctt ccaaggacgt cgattcaaaa cataccgagg
     1321 tatgggctca ttaggcgcga tggaaaaagg ttcaaaagac cgttacttcc aaggtagtgt
     1381 caatgaagca aataaattag taccagaagg aatcgaaggt cgggttgctt ataaaggaag
     1441 tgttgcagat attattttcc aaatgatcgg cggtttgcgt tcaggaatgg gttatgttgg
     1501 ggcagccaat ctacagcaat tacgtgatga agcgcaattt atccaaatga gcggcagtgg
     1561 attaaaagaa tcacaccctc atgatgtaca aatcacaaaa gaagcaccaa actattcagt
     1621 tgaatcttaa aaa
//
LOCUS       DACEUZ010000217         1594 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.220, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000217 DACEUZ010000000
VERSION     DACEUZ010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1594
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.220"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            <1..1485
                     /locus_tag="IXG44_002593"
     CDS             <1..1485
                     /locus_tag="IXG44_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAQ1659282.1"
                     /translation="CEAKFSENSYGFRPNRSVENAIKATYNRLQISQLHYVIEFDIKG
                     FFDNVNHSKLIKQIWAMGIRDKHLIFILKRILKAPIKMTNGTITYPEKGTPQGGIISP
                     LLANIVLNELDHWVESQWQENPVTKNYVVHINKSGSPCKSNAYKEMKKTKLKEMYMVR
                     YADDFRVFCRYKESAEKAKIAITQWIEQRLKLEVSQEKTRIVNVRKRYSDFLGFKIKM
                     IPRRKKLVVKSHISDKQFKNQKDKLVSQAKKIASPSKGKTELEETRLYNSMVLGMQNY
                     YQIATNVNLDCMKLNRAVMAIFTNRLGTNKRGRLRKTGRQLTTKEATRYGKSKMLRYV
                     AGEGEPIFPVGYIQHRKPMAKVYLANCYTLEGRKFIHTNLSVDKLLMYQLMKLSLENR
                     TIEYADNRISLFSAQHGKCAITLEEFQQANEIHCHHIVPTGAGGNDDYKNLILVKEAV
                     HRLIHAKTEETIQKYIALLKLNKVQLIRLNKYRVLAGNQELNLI"
BASE COUNT          632 a          251 c          307 g          404 t
ORIGIN      
        1 tgcgaagcta aatttagtga aaacagctat ggttttagac ctaataggtc agttgaaaat
       61 gcaataaaag caacctataa ccgcctacaa atcagccagc tacattatgt gattgaattt
      121 gatattaagg gtttctttga caacgtgaac cattccaagc tgataaaaca gatatgggct
      181 atgggaatac gggacaagca cttgatattc attttaaaaa ggatattaaa agctccaatc
      241 aaaatgacta acggtacaat aacatatcca gagaaaggaa ctccgcaagg gggaataata
      301 tccccactac tagcaaacat tgttcttaat gagttagacc attgggtaga aagccaatgg
      361 caggaaaatc ctgttacaaa aaactatgtg gttcatatca ataaaagcgg tagcccatgt
      421 aaaagcaacg cttacaaaga aatgaagaaa acaaagttaa aggaaatgta tatggttcgt
      481 tatgcagatg attttagagt tttctgccgc tacaaggaaa gtgctgaaaa agccaaaata
      541 gcaatcactc aatggattga acaacgatta aaattggaag tatcacaaga aaaaacaaga
      601 attgtaaatg ttagaaaaag atactccgac tttcttggtt tcaagattaa aatgataccg
      661 agaagaaaga agttagttgt taaatcacat ataagtgata aacaattcaa aaaccaaaaa
      721 gacaagctgg tatcacaggc aaagaaaatt gcttctccct caaaagggaa aacggaatta
      781 gaagaaacaa gactttataa ttcaatggta ttaggtatgc aaaattatta tcagattgca
      841 acgaatgtaa atctggattg tatgaaactg aatagagctg taatggctat cttcactaac
      901 agattaggta caaacaaaag gggcagattg agaaaaacag gcagacaact tacgacaaag
      961 gaggcaacaa gatatggtaa atctaaaatg cttcggtatg ttgctggcga gggcgaacct
     1021 attttccctg ttggatatat ccaacacaga aaaccaatgg caaaggtgta tttagcgaat
     1081 tgctacactc tagagggaag aaaatttatc cacaccaatc taagtgttga taaattactg
     1141 atgtaccagt taatgaaatt atctttggaa aatcgcacaa tagaatacgc agacaatcga
     1201 atttcgctat tttctgctca acatggaaaa tgtgctataa ctctggaaga atttcaacaa
     1261 gctaatgaaa tacattgtca tcatattgta ccaactggag ctggtggaaa tgacgattat
     1321 aaaaatctta ttttggtaaa agaggcggta caccgtctaa tacacgctaa aacagaagaa
     1381 acaatacaaa aatatatagc tctattgaag ctaaataaag tccagctcat aagactaaat
     1441 aaatatagag tattagctgg caatcaagaa ttaaacttga tataggtcta ctacataaat
     1501 acttttcaat ataaacacga tggaacgcgg agtgcggtga aagtcgcacg ctccgtgtga
     1561 atggggggaa aagtttgaga tatttataat atca
//
LOCUS       DACEUZ010000218         1583 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.221, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000218 DACEUZ010000000
VERSION     DACEUZ010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1583
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.221"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            28..645
                     /locus_tag="IXG44_002594"
     CDS             28..645
                     /locus_tag="IXG44_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011834674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1659283.1"
                     /translation="MWIFKQEIFAKLFSLDGLGWGILFFIFLFLGSMWIWFVDKKNSY
                     EKVIVKKMNRNEFDDFSKEHRVELYDSHEINSTSPSYYWVFGCEEKPLEIKLLKMLEN
                     PPVIEDFSAKNVEKQMFEQVQIRGQNKISLEPDEYAAFFLETNSGTSMPVFKVKLKMN
                     GRLCTYDLVANMRYGSTGDRRTILKFEQTFRSFLKNRVSQIFNLD"
     gene            809..1141
                     /locus_tag="IXG44_002595"
     CDS             809..1141
                     /locus_tag="IXG44_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1659284.1"
                     /translation="MNNREILAKEMADLKAKEAKIRAKRKQIRQKEMISLAKLYCKKE
                     GFKTPAEALDFLESNKRNHSVNDEVLVSLRKFADGLVWQENGAYWRTNDVKGLTQFMA
                     QFRSENVN"
     gene            1465..>1583
                     /locus_tag="IXG44_002596"
     CDS             1465..>1583
                     /locus_tag="IXG44_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1659285.1"
                     /translation="MAYMVARTQKHKVGSLNGLQNHVDRKTKNHTNKDIDITK"
BASE COUNT          570 a          186 c          313 g          514 t
ORIGIN      
        1 ttattttaaa gtgggaggaa aattgttttg tggatattta aacaagaaat ctttgctaaa
       61 ttatttagtt tagatggatt aggttgggga atcttatttt ttattttttt atttttagga
      121 tctatgtgga tctggtttgt tgataaaaaa aattcttatg agaaagtaat agtgaaaaaa
      181 atgaatcgaa atgaattcga tgatttttca aaggaacata gagttgaatt gtacgatagt
      241 catgaaatta acagtacttc tccatcttat tattgggtat ttggttgcga agaaaaacca
      301 ttggagatta aattgttgaa aatgcttgaa aatcctccag tcattgaaga tttttctgca
      361 aaaaacgttg aaaaacagat gtttgaacag gtacaaataa gaggtcaaaa caaaatttca
      421 ttagaaccag atgaatatgc agcctttttt ttagaaacaa attcaggtac ttcaatgcct
      481 gttttcaaag ttaaattaaa aatgaatggt cgtttatgca cttatgatct tgttgcaaat
      541 atgcgctatg gttctactgg tgatagacgt actattttaa aatttgagca aacctttaga
      601 agttttttga aaaatagagt atcccaaatt tttaatttgg attgaaaaaa ttaaaaattt
      661 ggccaaaaat actacagttt tttagagcaa tcatcttatg atgattgctc tttttttgtt
      721 gtttttgtgt cattcgttgt tcaaatcctc tattcgtaaa ataaatattt taaactagaa
      781 atatgcctta tttttaggag gaatcaaaat gaacaataga gaaatattgg caaaagaaat
      841 ggcggatttg aaagcaaaag aagcgaagat tagagcgaaa cgaaaacaga ttagacagaa
      901 agaaatgatt tctttagcga aattatattg taaaaaggaa ggctttaaga cacctgctga
      961 agcattagat tttttggaat caaataaaag aaatcattca gtaaatgatg aggttttagt
     1021 atcgctaaga aaatttgcag atggattagt ttggcaagaa aatggtgcat attggcgtac
     1081 gaatgatgtt aagggtttga ctcaatttat ggcacaattt cgttcagaaa atgtgaacta
     1141 aattgaggag gggttgaagt gaaaagcaat acttttcact tttggttttt tgcggaataa
     1201 aaattgtgaa aacaatgacg caaaaagcca atggttttga acgaaaaaag gacgtaaaaa
     1261 aggaatgcga aggcagtcgt ttttcgttcg ctttttcgtt tgagagaaac ggagtttctc
     1321 atcggctgtt aagccgatat agccctcggc agagcttaca gactttacgc agtataagtc
     1381 tagtaagtta taccttacat ggaggttttt ccgaatttag cttgtgattc tcgcaataat
     1441 tttaacgaaa ggaagtacac agttatggcc tatatggtgg cacgaacaca aaagcataaa
     1501 gttgggtcgt taaatggact tcaaaatcat gtagatcgta aaacgaaaaa tcatacgaat
     1561 aaagacattg atattacgaa aag
//
LOCUS       DACEUZ010000219         1582 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.222, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000219 DACEUZ010000000
VERSION     DACEUZ010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1582
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.222"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            292..1479
                     /locus_tag="IXG44_002597"
     CDS             292..1479
                     /locus_tag="IXG44_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAQ1659286.1"
                     /translation="MYFLGIDIGKRTHVASIMNEEGKVLLKGFSFPNTTEGAESLIER
                     MVDYSGAPSDFVIGMEATGHYWLSIFSYLHESDYLIHVVNPLQTDGWRKGTEIRKRKN
                     DIIDSVLIADLMRYGSFVETVLSDENVFSLKQLSRYRTYLVGTASDFKRKIIAVLDQV
                     FPEYATIFTKQGVFGKASKELLLAFSAPIDLENISSETLAQYLAELSRNRIKAEKADQ
                     LKQAASNSFGVKFAQEAFTFQLRSMIEQLKFIESQIKETEREIKHIMDTLNSVILTIP
                     GIGPINGATILGEIGDIQKFSNPKKLVAYAGIDASVTQSGQYEATHNVMSKRGSPYLR
                     KALFSAALVASQCDPVFSAFYQKKVSEGKHHLTALGAVSRKLCYVIHAILTKNEPYII
                     VSD"
BASE COUNT          467 a          296 c          315 g          504 t
ORIGIN      
        1 ttttaacctt ccggttttga ttggataacc ctatgaacca tcgttatttt aatttggtat
       61 taactcattt cttttgagat aatatattta ttggattgag aggctaagcc gttcccccct
      121 tgggccattg aacccgcact cgagagactg gctcctctct ctgataagtg gattgcgtat
      181 gagttagatg ttgaaagtcc ttactttact ccgtatctcg tacaaggtag tgacgctttt
      241 cagttgagtg gatttcaatc caaaattctt tttttgaaag gtcagatata catgtacttt
      301 ttaggcattg atatcggtaa acgcactcac gttgcttcta tcatgaacga ggaaggaaag
      361 gtattactaa aaggtttttc atttcctaac accactgaag gagcagagtc attaattgag
      421 cgaatggtcg actattccgg agcacccagt gactttgtta tagggatgga agccacgggg
      481 cactattggc tgtccatctt ttcatacctt cacgaatcag attatttgat tcacgttgtg
      541 aatccccttc aaacagacgg atggcgtaaa ggtacagaaa tacggaaacg aaaaaacgat
      601 attattgatt ctgttttgat tgctgactta atgcgctatg gctcatttgt cgaaacggtc
      661 ttatccgatg aaaatgtttt ttctttgaaa caactttctc gttaccgaac ttatttagta
      721 ggtacagcga gcgacttcaa aagaaaaatt atagccgtat tggatcaagt ctttccagaa
      781 tacgcgacga tttttaccaa gcagggcgtt ttcggaaaag cttctaaaga gttattgctg
      841 gcgtttagtg ccccgatcga cttagaaaac atttcctcag aaactttggc ccaatactta
      901 gctgagttaa gcaggaaccg aatcaaggca gaaaaagcgg accaattgaa gcaagcagct
      961 tctaactcct ttggagtaaa atttgctcaa gaagcgttca ccttccaact acgttcaatg
     1021 attgaacaat taaaattcat tgaaagccaa ataaaggaaa cggaaagaga aatcaagcac
     1081 ataatggaca ctttaaattc tgttattctc acaattcctg gaattggtcc tataaacggt
     1141 gcgactattc ttggtgagat cggagatatt caaaaatttt ctaacccgaa aaaactcgtt
     1201 gcttacgctg ggatagacgc ttccgtgaca caatcagggc aatatgaggc aacgcataat
     1261 gttatgagta aacgagggtc tccatatttg cgaaaagcct tgttttcggc ggctttagta
     1321 gcttctcaat gtgatcctgt ttttagtgcg ttttaccaga aaaaagtttc cgaaggaaaa
     1381 catcatctaa ctgccttagg tgcggtttct cgtaagcttt gttacgtgat tcatgctatt
     1441 ttgactaaaa acgagcctta tataattgtt tctgattagc tattgaacat tcaaaaaaac
     1501 taatggtaag aagcttattt gttgtaccct tttttcggaa ttatctgtta taaaatattt
     1561 tttgatttgg ctaattttta ct
//
LOCUS       DACEUZ010000220         1515 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.223, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000220 DACEUZ010000000
VERSION     DACEUZ010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1515
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.223"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            287..694
                     /locus_tag="IXG44_002598"
     CDS             287..694
                     /locus_tag="IXG44_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1659287.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            708..1109
                     /locus_tag="IXG44_002599"
     CDS             708..1109
                     /locus_tag="IXG44_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1659288.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1111..1482
                     /locus_tag="IXG44_002600"
     CDS             1111..1482
                     /locus_tag="IXG44_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1659289.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
BASE COUNT          575 a          185 c          264 g          491 t
ORIGIN      
        1 tgaggggaag tacatatagt tgttgatata gttgctaata ttgttagcta atttgttagt
       61 aaaactgtat acgaatttag atagtaaaaa aacagtttgt tccttatata gaggcacttt
      121 tactactgat taaatgtcag atatgcttac aaatatactt ataaatatgc ttacaaaatt
      181 agctaatatt tccgttgaca aagctttgtt actattgtta aactaaattg actagcaaaa
      241 gtgaaacata aatttattaa ataaatgtaa aaattaggag aaatcaatgt cttatagagt
      301 acaattaata atttcagaag atgtagaaag tcagcaattt ggcactaatg ttattaataa
      361 agtaattaat ccagctcttt ctattaatgc gcctttaatt cctactgctt tatcattttc
      421 tgtaacagct gtggtgtctg agatagagga tacagaaaag ataaaaatag tagaaattga
      481 agttttaaac aagaatgaaa aacaaatttt ttctacgggt gaagtatcgg taagcttgcc
      541 tcctcaagtt aacgatataa actttaatat aaacgccaga aatgtcttgg ttgaagaagc
      601 aggagagcat tatgctgttg ttaaatttaa tggaactgag attggaaggc aaatctttga
      661 tatcaaggtc aacaaaccag tggaaaaaaa ttaaggagac gatacggatg cttgatatag
      721 taccatcaaa tacacatcgg agtggtagct taataaattt tgctgcgatt tttgcttgcg
      781 catcggtaat tctagcatct ccagttgttg tggatgcaag ttctactccg aaaactaaaa
      841 atgataatca acttgtaatt actacaaaaa atgagattaa cacaatttca agtaatgatg
      901 gcaaaatttt tgatgtgata aatacggttt taaaaagaaa agctgaaatc aatataaaat
      961 atgatgaaga tttaaacttg tatttctttg ttatcaaaac aacatctgaa ttgttttcct
     1021 cagattacga tgttttggat actctcgata atgtattatc tgactataaa tatatgggga
     1081 aatctgtagt agcaactttg ggggaataaa atgtttgact ttaatgaata tttatctatt
     1141 tccaaacaaa ttgaatcaga tgataaatat aattctaaag aatcttatag aagaactgct
     1201 gtttcaagag catactacag tgcttttaaa aaatctgatg agtatttgaa agaaaattat
     1261 gatattattt ataatggaag tagcggaaaa ggaagccacc aaaccgtttg gaatttgttt
     1321 tcaacagtta aggaattgaa taccttagga atacataata gtgggtatag aatgttagag
     1381 aaaagaaaat gtgctgacta tattcctaat gaaaccatta cgaaaacaga tatggcgctg
     1441 atgaatcgag aagcagaaaa aataataaac aaactcacct aatgaatttc aagcgagttt
     1501 gtttgttatt ttttt
//
LOCUS       DACEUZ010000221         1456 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.226, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000221 DACEUZ010000000
VERSION     DACEUZ010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1456
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.226"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(<1..1204)
                     /locus_tag="IXG44_002601"
     CDS             complement(<1..1204)
                     /locus_tag="IXG44_002601"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HAQ1659290.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRIS"
BASE COUNT          422 a          305 c          249 g          480 t
ORIGIN      
        1 ctgagatacg acgtacatag ttacgtgcgt tttctggcag ttctgcaagt gttttgcatc
       61 ctgtaatgtc ttctgaccag cctggcaatt cttcatagat tggtttgcag cggttcaact
      121 ctttcaagct tgctggatag tggtagatta gttcgccatc taattcataa gccgtacaaa
      181 tttttactgt ttccaatcca cttaatacat cgatcgagtt taaagatagg ttggtaatac
      241 ctgaaactcg tttagaatgg cgcataacta cagaatcaaa ccagcctaca cgacgcggac
      301 gaccagttgt cgtaccgtat tctcgtccta cttcgcggat ttgttctcct gtttcatcaa
      361 ataattcagt tggaaatggt ccatcaccga cacgtgacgt ataggctttg cagacgccga
      421 caactttatt gatttttgaa ggccctacac cgcttccgat tgttacccca ccggctactg
      481 ggttagaaga agtaacaaaa ggataagttc cctggtcgat atccagcatt acaccttgtg
      541 cgccttcaaa caacacgcgt ttgcctgcat ccaaagcatc attcaagata acggaagtat
      601 ctgtgacgta ttgtttgatt tgttgtccgt attcataata ttcttcaaaa atatcgtcaa
      661 aatcaagtgg ttctgaatca aacattttta caaattgacg gtttttgtca tctagattca
      721 aacgaagacg ttcttcaaaa atttctttat ctaataagtc tgcaatacgg atacctacac
      781 gagctgcttt atccatatag gcaggtccga ttcctttaat cgttgtacca atcttatttt
      841 cgcccttagc atcttcttgt aattggtcca atttgatatg ataaggcaag attacgtgcg
      901 cgcggtcaga aatacgcaaa ttacttgttt ctacaccttt ttcatgtaaa taagctaatt
      961 ctttaaccaa tgatttagga ttgactacaa cgccgtttcc gatcacactg atcttgtctt
     1021 tataaaaaat cccagaagga atcaaatgca gtttataagt cacaccgtta aacttaattg
     1081 tgtgccctgc attgtcgcca ccttggtacc gtgcaatcac ttcagcattt tcactgagaa
     1141 agtctgtgat ttttcctttc ccttcatcgc cccattgcgt acctactact actacagatg
     1201 acatgtgaac acctcgttct atattattta atcctgaacc attgtatcaa taatagatac
     1261 atatttcaaa taaaatcgaa tgatttttat ttttatttta taaaaacgct taaaaaacga
     1321 actttatctt tttagaatgg ttattaaaaa agaggttgta acagaagtgg tcagcttcaa
     1381 gaaataagaa ggaatgtacg aaaattgctt ctcaaatttt tgtgaaattt cgacttattt
     1441 ttcgaagaag ctactt
//
LOCUS       DACEUZ010000222         1455 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.227, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000222 DACEUZ010000000
VERSION     DACEUZ010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1455
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.227"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            108..1040
                     /locus_tag="IXG44_002602"
     CDS             108..1040
                     /locus_tag="IXG44_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HAQ1659291.1"
                     /translation="MLKLKEKEYIIDDAVSVPLILNKREKNNLKKKMLIPFDSEVKGL
                     LNKLTISNIDSTREKIIKIFDKINNLFKNDNAELRKGLLEFKLQELELHYSYLVKITE
                     EKEQQKAIKEQMIEEEKVRREIDREKKRIDKEQRQFNSEISKLIAYMQKANADVEKEL
                     YANKIQELEEKLKELEVIRENVLQRELNTRAGYVYVISNIGSFGEDIYKIGMTRRLEP
                     MDRIKELSSASVPFEFDVHAMIFSEDAPSLETKLHNHFRKQEVNKINQRKEFFKVSLD
                     EIEKVVLENYNGTVTFTKLAKAEQYRRSLELSKD"
     gene            1112..>1455
                     /locus_tag="IXG44_002603"
     CDS             1112..>1455
                     /locus_tag="IXG44_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1659292.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDD
                     VLVKNVNSNLIQ"
BASE COUNT          640 a          169 c          227 g          419 t
ORIGIN      
        1 atgaaataaa tcaaaagtat gatgaaacat taaaaaatgt ctattcttca cttatcgttt
       61 caaatataga agaaacttca tcttctgaaa taaaaaataa gctttctttg ttaaaattaa
      121 aagaaaagga atatattatt gacgatgctg tttctgtccc tcttatttta aataaacgag
      181 agaaaaataa tttaaagaaa aaaatgctca ttccatttga ttcagaagtg aaaggattac
      241 tcaataaact aactatttct aatatagact cgactaggga aaaaatcata aaaatatttg
      301 ataaaattaa taatttattc aagaatgaca atgctgaatt aaggaagggt cttttagagt
      361 ttaaacttca agaactagaa cttcattact cttatttagt gaaaattacc gaagaaaaag
      421 aacaacaaaa agcaataaaa gaacagatga tagaagagga aaaagttcgt agagaaattg
      481 atcgtgaaaa gaaacgtatt gataaagaac aacgtcaatt caattctgaa atttccaaat
      541 taatcgcata tatgcaaaaa gctaatgcag atgtggaaaa agaattatat gcaaataaaa
      601 ttcaagaact ggaagaaaaa ttaaaagaat tagaagttat acgtgaaaat gtattacaaa
      661 gagaattaaa tactagagcc ggttatgtgt atgtaattag taatattggg tcttttggtg
      721 aagacatata taaaattggt atgactagaa gattagaacc tatggataga ataaaagaat
      781 tgagtagtgc atcggttcca tttgaatttg acgttcatgc gatgattttt tcagaggacg
      841 ctccgtcttt ggaaactaaa ttgcataatc attttagaaa acaagaagtt aataaaatta
      901 atcaaagaaa agaatttttc aaagtttcac tagacgaaat agagaaagtt gttttagaaa
      961 attataatgg aacagtaaca tttacaaaat tagctaaagc tgaacaatat agacgatctt
     1021 tagaattgtc aaaagattaa atatataaaa agccttcggg ctttttcttt taacaattaa
     1081 taggaacgta tattcgaaag gaatagaaat aatgtggtta gaaaaaataa atgacaaaaa
     1141 atttaagtac accgaacgtt atacggatcc cttaactgaa aaaaagagaa aagtgtccgt
     1201 tactttatct agcaattcac gccaagcttg gaatcaagct aacttacttt tgaatgaaaa
     1261 aatcgcagaa aaaataaaac gaaatgaaga attaccacta acttttggag aattaaaaac
     1321 taaatgggat gaaaaataca agccaactgt aaaagaaagt tcctatcgaa caactcaggt
     1381 ttatctatca ttaatttcaa aatatataaa agatgatgta cttgtaaaaa acgttaatag
     1441 taatcttata caaga
//
LOCUS       DACEUZ010000223         1445 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.228, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000223 DACEUZ010000000
VERSION     DACEUZ010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1445
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.228"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            <1..226
                     /locus_tag="IXG44_002604"
     CDS             <1..226
                     /locus_tag="IXG44_002604"
                     /inference="COORDINATES: protein motif:HMM:NF018513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HAQ1659293.1"
                     /translation="LWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKIIKEAIPSLLLI
                     VIIIGGILTGIFTAVEASAIAVIYKWVIRF"
     gene            208..513
                     /gene="kduI"
                     /locus_tag="IXG44_002605"
                     /pseudo
     CDS             208..513
                     /gene="kduI"
                     /locus_tag="IXG44_002605"
                     /inference="COORDINATES: protein motif:HMM:NF002091.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
     gene            533..1336
                     /locus_tag="IXG44_002606"
     CDS             533..1336
                     /locus_tag="IXG44_002606"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HAQ1659294.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIRDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
BASE COUNT          462 a          259 c          344 g          380 t
ORIGIN      
        1 tttgtgggcg ctaggaatca tgctagtagc attcatcgtt gctaaaaaga ataaataccc
       61 tgttgtcgaa aaaggacaga tggctcatgc tggaaaaata ataaaagagg cgattcctag
      121 ccttctattg attgtcatca ttatcggtgg tatcttgacg gggatattta cagcagtaga
      181 agcttcagcg atagctgtca tttataaatg ggttatacga ttttagaacc aggaagtgct
      241 tggaatacga tgccttgtca tacacatgaa cgtcgtatgg aagcttatgt ctattttgat
      301 tatgccaatg aagatacacg tgtgttccat atgatgggca aaccagatga aacaaaacat
      361 ttagtcgtag acaatgaaca agccattatt tcgccaagct ggtcgatcca ttcaggtgta
      421 ggtacaagca attactcatt catctgggca atgtgcggag aaaatatcac ctatacagac
      481 atggatatgg tgcaaatgga tcaattaaaa taagcataag ggagagaaaa tgatggaatt
      541 tagtatggat atgtttcgtt tagatggaaa agtagcatta gtcaccggtg ctgtctatgg
      601 tattggattt gagattgcac gctctttagc tacagcagga gcaaaaatcg tatttaataa
      661 cttgaatcaa gaatcagttg atgaaggtat agcgcattat aaagaagcag gcatcgatgc
      721 aaaaggttat gtctgcgatg tgaccgatga agaagcggtt caagcgatgg ttcaacagat
      781 caaagaagat gtaggttcag ttgatattct agtcaacaat gcagggatca tcaaacggac
      841 accgatgatc gaaatggatg cggcagactt ccgccaagta atcgacgtgg atttaaatgc
      901 cccattcatc gtttcaaaag cagttattcg ggatatgatc gaaaaaggcg gcggtaaaat
      961 catcaatatc tgctccatga tgagtgaatt aggacgcgaa acggtcagtg cttatgctgc
     1021 ggcaaaaggc ggattaaaaa tgctgacgaa aaacatcgct tctgaatacg gtcaatacaa
     1081 tatccaatgt aatggaattg gaccaggtta tattgctaca ccgcaaactg ctccattacg
     1141 cgaaacacaa gaaaacggtg aacgtcatcc attcgatcaa tttatcgttg gacgtacgcc
     1201 agcagcacgc tggggcgagc caatcgattt ggctggacca tctgtcttct tggcttctag
     1261 cgcatctgat ttcgttaacg gccatgtctt gtatgtggac ggaggcatac ttgcctatat
     1321 cggaaaacaa ccttaaaaga gctttagtac accgtttagt ggagaggttg tgaaatcagc
     1381 ggcttgaacg aataccgaag gacagctgat taaacatcat ttattcgaaa aaagaggctg
     1441 ggaca
//
LOCUS       DACEUZ010000224         1424 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.229, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000224 DACEUZ010000000
VERSION     DACEUZ010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.229"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            8..772
                     /locus_tag="IXG44_002607"
     CDS             8..772
                     /locus_tag="IXG44_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HAQ1659295.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVIPLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            895..1398
                     /locus_tag="IXG44_002608"
     CDS             895..1398
                     /locus_tag="IXG44_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ1659296.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TELSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
BASE COUNT          490 a          228 c          341 g          365 t
ORIGIN      
        1 gaggaatgtg aaagagatcc aatcagcaaa aaatccaaga atcaaagaat taaaaaaact
       61 acataaaaag aaatatcggg aagaaaagga agaatatctt ttggaagggt tccatctgat
      121 tgaagaagct gcgtcgtctg atgcagaaat aaaagaactt tttgtgacac agcgaggcat
      181 gaaagaatgg ggagactggc tggccgcaaa caaagagatt tctacttttc tagtttcgga
      241 tgaggtcatg gatgctgtat cggatctacc tacaccacaa ggaatcttag cagtgatggc
      301 gttaacggct gaaggcacgc cagatttttc aggaggatgg ctgttgttag ataatgtaca
      361 agatcctgga aatgtaggga cgatgatccg tacggcagat gcatttgata tggctggtgt
      421 tttattaggc aaaggaacgg cagatattta tagtacaaag gtgctgcgca gcatgcaggg
      481 aagcaattat catttgcctg tgttgcaaaa agatttgaca gaagtgattc ctttaatgaa
      541 agacaaaggg tttacagtgt atggcacaga attaaatgaa gaagcacacg cattagacaa
      601 agtagaaaaa tcagataact tcgcaataat catggggaac gaaggtcagg gggtaagtca
      661 ggaaatcttg tctttgactg atcagaacgt atatatcccg atgaaaggaa atgcggaatc
      721 gttgaatgtt ggggttgcgg cagggattgt tctttatcat ttttctttat gattaaggaa
      781 ctattaagct ttggctattt tatccaaggc gactagaata atccgaacac tggtggtata
      841 cttagatcaa tttcagaaga aaatgaaatt caaaaaggtg gcagatcgat aattatgaca
      901 gaaaaatggc gtgaagacga agaatatatg tcttatgtag aagatctttt agaaacagaa
      961 gcagtgaaaa aattagcgaa ttacacacag catgttcatt ccactcgttt ggagcattcc
     1021 atcagtgttt cttactatag ttatctgctc gcaaaaaaat ggggtggcaa tgctaaagca
     1081 acagcgcgtg ctggactttt gcatgatctg ttctactatg attggcgtac gacgaaattt
     1141 gatgaaggca cacatgcgta tatccatcca agaattgctg taaagaatgc agaaaagatc
     1201 accgaattgt cggatcttga gcgagatatc attttgaagc acatgtgggg cgcaacgatt
     1261 gcaccgccta agtacaaaga aggatatatc gtaacattcg tagacaaata ctgcgcagtc
     1321 aaagaagccg ctcagcctat gtcagcaagt atgcgtaaga gatggcaacg ttactttggg
     1381 aaagaatcct cgatttaata gataaaacca ataatagacc atat
//
LOCUS       DACEUZ010000225         1424 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.230, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000225 DACEUZ010000000
VERSION     DACEUZ010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.230"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(150..1337)
                     /locus_tag="IXG44_002609"
     CDS             complement(150..1337)
                     /locus_tag="IXG44_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HAQ1659297.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          414 a          309 c          249 g          452 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttcact ttta
//
LOCUS       DACEUZ010000226         1355 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.232, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000226 DACEUZ010000000
VERSION     DACEUZ010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.232"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            <1..>1355
                     /locus_tag="IXG44_002610"
     rRNA            <1..>1355
                     /locus_tag="IXG44_002610"
                     /product="23S ribosomal RNA"
BASE COUNT          362 a          295 c          415 g          283 t
ORIGIN      
        1 caagctgtga tggggaggga aataatagta ccgaagttcc tgatgtcaca ctgccgagaa
       61 aagcttctag tgagaaaaca gcggcccgta ccgcaaaccg acacaggtag tcgaggagag
      121 aatcctaagg tgagcgagag aactctcgtt aaggaactcg gcaaaatgac cccgtaactt
      181 cgggagaagg ggtgctgatc atacgatcag ccgcagtgaa taggcccaag cgactgttta
      241 tcaaaaacac aggtctctgc aaaatcgtaa gatgaagtat aggggctgac gcctgcccgg
      301 tgctggaagg ttaagaggag tgcttagcgc aagcgaaggt acgaattgaa gccccagtaa
      361 acggcggccg taactataac ggtcctaagg tagcgaaatt ccttgtcggg taagttccga
      421 cccgcacgaa aggcgtaacg atttgggcac tgtctcaacg agagactcgg tgaaatttta
      481 gtacctgtga agatgcaggt tacccgcgac aggacggaaa gaccccatgg agctttactg
      541 tagtttgata ttgagtgtct gtaccgcatg tacaggatag gtaggagccg tagaaatcgg
      601 aacgctagtt tcgatggagg cgctggtggg atactacccc tgcgttatgg ccactctaac
      661 ccgcaccact aatcgtggtg ggagacagtg tcagatgggc agtttgactg gggcggtcgc
      721 ctcctaaaag gtaacggagg cgcccaaagg ttccctcaga atggttggaa atcattcgaa
      781 gagtgtaaag gcagaaggga gcttgactgc gagaccaaca agtcgagcag ggacgaaagt
      841 cgggcttagt gatccggtgg ttccgcatgg aagggccatc gctcaacgga taaaagctac
      901 cctggggata acaggcttat ctcccccaag agtccacatc gacggggagg tttggcacct
      961 cgatgtcggc tcgtcgcatc ctggggctgt agtcggtccc aagggttggg ctgttcgccc
     1021 attaaagcgg cacgcgagct gggttcagaa cgtcgtgaga cagttcggtc cctatccgtc
     1081 gcgggcgttg gaaatttgag aggagctgtc cttagtacga gaggaccggg atggacttac
     1141 cgctggtgta ccagttgttc tgccaagggc attgctgggt agctatgtag ggaagggata
     1201 aacgctgaaa gcatctaagt gtgaagccca cctcaagatg agatttccca tttctttaag
     1261 aaagtaagat ccctgagaga tgatcaggta gataggtcag gagtggaagt acagtgatgt
     1321 atggagcgga ctgatactaa tcgatcgagg actta
//
LOCUS       DACEUZ010000227         1348 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.233, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000227 DACEUZ010000000
VERSION     DACEUZ010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1348
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.233"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            <1..330
                     /locus_tag="IXG44_002611"
     CDS             <1..330
                     /locus_tag="IXG44_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1659298.1"
                     /translation="DFKPLGLAIKEARLSKGLTREQVGSMIQIAPRYLTNIENKGQQP
                     SFHVVYDLVTLLNISLDGFILGEENSHKSSKRLQVEAQLDCLDEDELVTIGKVIQAIY
                     GEKCKFN"
     gene            complement(844..1011)
                     /locus_tag="IXG44_002612"
     CDS             complement(844..1011)
                     /locus_tag="IXG44_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ1659299.1"
                     /translation="MCPICGNKTRLKMREDTELKNFPLFCPKCRQEILIEITKFRITV
                     ITEPDAKTQSR"
     gene            complement(1099..>1348)
                     /locus_tag="IXG44_002613"
     CDS             complement(1099..>1348)
                     /locus_tag="IXG44_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011997530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein"
                     /protein_id="HAQ1659300.1"
                     /translation="KYCANILNTKLKGNEVILIGEIPARCIQEYRNSLTFFTNGRSVC
                     LTELKGYQVTNIKSAFQPRRPNNRIDKVRHMFNKINLH"
BASE COUNT          440 a          204 c          227 g          477 t
ORIGIN      
        1 gatttcaaac cattgggtct tgcgattaaa gaagcccgtc ttagtaaagg attgactcgt
       61 gagcaggtag gatcaatgat ccagattgcg ccacgctatt tgacgaacat tgaaaataaa
      121 ggacaacagc caagttttca tgttgtatat gatttagtga ctttgctaaa tatttctttg
      181 gatggattta ttttagggga agaaaacagt cataaaagct ccaaacgtct tcaagtagaa
      241 gcacagttgg attgtctgga tgaagatgaa ttagtgacga ttgggaaagt gattcaagca
      301 atttacggag aaaaatgtaa atttaactag ttcaataata cacaatggat taattaatac
      361 tatcaggaaa gattgattac aaagatgtaa tgaatttttt ctgatttttg ttaatcataa
      421 atagaaaatg aatttatctt tgttacactt aggaactaga gcgaatgata aatagttctt
      481 tggttactct tatcgctgca aagtacagtt cattttttgt tgttgggaaa attttctgga
      541 tttattgcat atcacttcat ccgactaaaa atttttgatt cccatagcga agttcgtttt
      601 tgttccttag gaaaaacttg actgatatac tagttttgaa atgacgagta actctgtact
      661 tcatcaataa tcaagtattg aattttaatt gtatactttt tatgcttgag ggatgtctac
      721 tgtaatacag ctactgcaaa aaaaatcagt acacttattt tcttataaag gatatcaaga
      781 actaagctca atggtatgca tagaaaagaa aaagccgata aaatgagatt ttgatatctc
      841 gaattatcgg ctctgcgtct ttgcgtctgg ctctgtaatt acagttattc taaacttcgt
      901 tatttcaatt aaaatttctt gtctgcattt aggacaaaat agagggaaat tttttaattc
      961 agtatcttcc ctcattttta aacgggtctt attgccacat atagggcaca gtatccacct
     1021 agtatttaca atatctatat cctcctgttc taattaacaa ctcaatattc attttaaaaa
     1081 tttctcttta gttcactatc aatgtaagtt gattttatta aacatatgcc ttactttgtc
     1141 tattctatta tttggacgac gtggttggaa agcagactta atgttagtaa cctgataacc
     1201 ttttaactct gttaaacaga cactgcgtcc atttgtaaag aaagttaaac tgtttcgata
     1261 ctcttgaata caacgggctg gaatttcacc aatgagaatg acctcgttac cttttaactt
     1321 agtatttaaa atatttgcac aatatttg
//
LOCUS       DACEUZ010000228         1336 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.234, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000228 DACEUZ010000000
VERSION     DACEUZ010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1336
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.234"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(146..1129)
                     /locus_tag="IXG44_002614"
     CDS             complement(146..1129)
                     /locus_tag="IXG44_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HAQ1659301.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKDPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILETFEKEHPTLFSEQ"
BASE COUNT          377 a          285 c          254 g          420 t
ORIGIN      
        1 atataaacgg tgttcaatca actgaccctc ggtattagct caaaaaacga caaaatatga
       61 agagctattt ttgtttggct gttcttatta cttgatacag gacagctttt tcacgacctc
      121 taattcacgg tgttcaagca agaaatcatt gttcactgaa aagcgttgga tgttcttttt
      181 caaaggtctc taagatctct cgtacttcat cagtagattt cgtattcatc aactgaactc
      241 gcaagtcatt tgctcctgga aatcctttga tatagatttt aaagaaacga tgtagtcctg
      301 tgatggaacg cgggatgatt tctgaataat gatcttgcag atctagttgc agacgaagta
      361 gtccgatcag ttctttttct gtatgttccc tcgggtcttt ttcaaatgca taaggattct
      421 tgaaaatccc tcggccaatc atgatgccat caacgccata tttctcggcc aattcttctc
      481 ctgtctggcg atcggggata tctccattga tcgtgattag tgtttgtgga gcgatttggt
      541 cacgtaaagc aatgatttta ggaatgactt cccaatgtgc atctacttta ctcatctctt
      601 tacgcgtacg caaatggaca gaaagattgg ctatatcttg atgaagtagg tgactgatcc
      661 attcttccat ctcagacatt tccgtatagc cgatacgcgt cttgacgctg acaggcaaac
      721 cgccagcttt agctgcttca atcaattgtg ccgctacttc tggacgaagg atcaagccac
      781 tgccttttcc acgttctgct acattaggga ctgggcagcc catgttgata tcgattcctt
      841 ggaagcccat ttctgccata gcaatgctca tttctctaaa aaattctggc ttgtctcccc
      901 aaatatgggc aaccatcggc tgttcatctt ctgtaaatgt taatcttcct cgtacactgt
      961 cttttccatc tggatgacag aaactgtctg agtttgtaaa ctcagtaaag aagacatccg
     1021 gtgctgctgc atgctttacg acatggcgaa atacaacatc tgttacatct tccattggtg
     1081 ctaaaacaaa aaaaggcttt ggcagctctt tccaaaaatt agtagtcatt tatttgttac
     1141 tctccattct atcaagcagt gaagtcgcaa tctttctcaa aaacattgtc attatactag
     1201 gaaacaaaaa aagaagcaaa gtgaacagct taaaagatgt ttctgattga gcaaaaaaaa
     1261 gactgtgaca aaaatcttat cacagtccat tttccggaat aaatggtgtt caaaagcttg
     1321 catttgcggt aataag
//
LOCUS       DACEUZ010000229         1263 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.238, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000229 DACEUZ010000000
VERSION     DACEUZ010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1263
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.238"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            849..1061
                     /locus_tag="IXG44_002615"
     CDS             849..1061
                     /locus_tag="IXG44_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1659302.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
BASE COUNT          470 a          189 c          209 g          395 t
ORIGIN      
        1 taaatattat acagataata acggaatatt atcatcgaaa cagctaacaa aattgtcata
       61 aaaaacgaga tggttgatat tagaacttgt gtttttgttt aggtctagag ataaagagag
      121 acaatttcca aatatatgta atgaatacaa aaagacgttt gtcgcggatt accgttacaa
      181 acgtctttta ttgattttcc gagatctcat cagtcgccat ttggaatggt tcactcctta
      241 tctagattcg tttaatgctc ggactgcttg ttcgattgta ataccaaacg caacgttatt
      301 ttcgttttgt gcatcaatcg ccataaaacg gttagaagtt gtatcaaaag ctacgcggta
      361 ttgcatattt gtttttgtaa acgtcataaa attcatctcc tttgtaatgt aatttagttc
      421 ttgaaagttt cctttaaact ttcgaactta taccaagttt agcatcgtac gttttttgat
      481 gcaattaaaa agcatttatc attttcctta tttatttttt cttaaatggt ttagctattt
      541 tcttttaatc cgataagtag cccaatcatc aaggttacaa atactattgc ttgaatataa
      601 atcatatgct cacctcttta taaataactt agcaatataa agaaaaaaac gcacgaacaa
      661 cataggaata taaacaaacg tttttgtcac aacgtctaag atcgtgttaa aaaatagtta
      721 tctaaaaagc agaaagatac tcaaaataaa aaataaaaaa atggataaaa acgttttcaa
      781 atacaaggta atttgttatt ataaaataaa gaaaaaacaa aataaaaata aagaaaagag
      841 ggaatgaaat ggtaaaagca gaagtaggaa gatcttatat atgcaaaccg atcggagtat
      901 ctcatgaact aatcggctgt attgaacgta catatatcaa cacggctgtc gtgtatgttg
      961 aaagttatga ccaacgagac tatcaactta tcaatgaaag caagtatcgc atgctcgtaa
     1021 aattctgtga tattagcgca cctgctgaat tggtttctta aacagtattt ttgagaattc
     1081 gcaagaactc ccttataata taaataaaga cagaagggag cgagaagata tgccaaaaag
     1141 aaaaagttca gggaaaatgg gaattattct tgcagtgatc tctgcaattc ctgctatttt
     1201 agctattgtt cgtgcgatga aggaatcaag agaaagttaa gcacacatta tgagcgtcaa
     1261 aca
//
LOCUS       DACEUZ010000230         1247 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.240, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000230 DACEUZ010000000
VERSION     DACEUZ010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1247
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.240"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            200..742
                     /locus_tag="IXG44_002616"
     CDS             200..742
                     /locus_tag="IXG44_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1659303.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            832..>1247
                     /locus_tag="IXG44_002617"
     CDS             832..>1247
                     /locus_tag="IXG44_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HAQ1659304.1"
                     /translation="MDELKRTEIQIIDKKERPKNGFVLSEKTDLENTEAFNKESVKDL
                     RKLPTKDINGKDFTSKDFEKYDLTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIV
                     GVVTDPVDDNGENKEAIEKSKLIHEKTKASYPFLMP"
BASE COUNT          515 a          141 c          238 g          353 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tctggatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccaga
//
LOCUS       DACEUZ010000231         1187 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.241, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000231 DACEUZ010000000
VERSION     DACEUZ010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1187
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.241"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            141..398
                     /locus_tag="IXG44_002618"
     CDS             141..398
                     /locus_tag="IXG44_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1659305.1"
                     /translation="MSNLENKEEKVVNKIVSVVNKLDKELDELDTLSENPEKKHNLKK
                     WLVERKAIHEIKKVLHEADKYEKYDEKELDKEFKEINDLLL"
     gene            complement(630..1043)
                     /locus_tag="IXG44_002619"
     CDS             complement(630..1043)
                     /locus_tag="IXG44_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HAQ1659306.1"
                     /translation="MDQKAFIESIDSTIDRLKKEIRFYNRVIGIGNIIKIVLSASIPI
                     LIDQASEHRSLLLIVSIASAIITIIQSGMSAFNYQDKVQTSTELLMKIEKEKLLYITK
                     TSPYNKTDEENFHLIVTTLQTELNDIISDFNQVNN"
BASE COUNT          453 a          150 c          148 g          436 t
ORIGIN      
        1 ttctgtccac gttcatttta ctccaaagtt ttcctcattt caaaagaaaa tcacagaata
       61 cacgaagaag cgtttctatt tgtataaact atatcaaagt tctatactta aattatcaaa
      121 taaaaaggac gtgaagataa atgtcaaatt tagaaaacaa agaagaaaaa gttgtaaaca
      181 aaattgtttc tgttgtcaat aaactggata aagagttaga cgaacttgat acgttatctg
      241 aaaatccaga aaaaaaacat aacctaaaaa aatggttagt tgaacgaaag gctattcatg
      301 aaattaaaaa agttcttcat gaagctgata aatatgaaaa atatgatgaa aaagaacttg
      361 ataaagaatt taaagaaatc aatgacttat tactataatt gatttatacg actcattttt
      421 gagtcgtcag actgcaaaca acactctaaa tttaggagtt tgtctacagt ctgaataaat
      481 tttaaattta ttgtccataa tgttattatg aatttatagc attaagaaaa gagataagac
      541 tttacatttt tttaggaatg taaagtctta tctcttttct taattgtaca attagtatta
      601 tctgcactac tttctaatta aataaatttt tagttattaa cttgattaaa atcagagata
      661 atatcattta attcagtttg aagggttgta acgattaaat ggaaattttc ttcatctgtt
      721 ttattatatg gggatgtttt cgttatatat aacaactttt ctttttcaat tttcattaat
      781 aactctgtcg aagtctgtac tttatcttga taattaaatg cagacatacc actttgtata
      841 attgtaatta ttgctgaagc aattgaaaca atcaatagta aagacctgtg ttcggaagcc
      901 tgatcaatta atattgggat actagctgat aagactattt ttataatatt tccgatacca
      961 attactctat tataaaatcg aatttctttt tttaatctat cgattgtaga atcaatagat
     1021 tcgataaacg ctttttgatc catcattttc tctccaatca ttttattaaa atcattaaca
     1081 aaaatattat aataggattt aagtaagttc taactaatca catataaaaa taaaaaaaca
     1141 tttgtatttt atcaaagttt tattatactt gtcaatttgt gtacatc
//
LOCUS       DACEUZ010000232         1137 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.244, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000232 DACEUZ010000000
VERSION     DACEUZ010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1137
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.244"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(<1..117)
                     /locus_tag="IXG44_002620"
     CDS             complement(<1..117)
                     /locus_tag="IXG44_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine methyltransferase"
                     /protein_id="HAQ1659307.1"
                     /translation="MSRFFKFGKLHVTKGNGDKLLDILLTASKKLKRSLAPTG"
     gene            complement(122..859)
                     /gene="erm(B)"
                     /locus_tag="IXG44_002621"
     CDS             complement(122..859)
                     /gene="erm(B)"
                     /locus_tag="IXG44_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAQ1659308.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNIRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(984..1067)
                     /locus_tag="IXG44_002622"
     CDS             complement(984..1067)
                     /locus_tag="IXG44_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HAQ1659309.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
BASE COUNT          343 a          185 c          184 g          425 t
ORIGIN      
        1 ccccgtaggc gctagggacc tctttagctt cttggaagct gtcagtagta tatctaataa
       61 tttatctcca ttccctttag taacgtgtaa ctttccaaat ttaaaaaagc gactcataga
      121 attatttcct cccgttaaat aatagataac tattaaaaat agacaatact tgctcataag
      181 taacggtact taaattgttt actttggcgt gtttcattgc ttgatgaaac tgatttttag
      241 taaacagttg acgatattct cgattgaccc attttgaaac aaagtacgta tatagcttcc
      301 aatatttatc tggaacatct gtggtatggc gggtaagttt tattaagaca ctgtttactt
      361 ttggtttagg atgaaagcat tccgctggca gcttaagcaa ttgctgaatc gagacttgag
      421 tgtgcaagag caaccctagt gttcggtgaa tatccaaggt acgcttgtag aatccttctt
      481 caacaatcag atagatgtca gacgcatggc tttcaaaaac cactttttta ataatttgtg
      541 tgcttaaatg gtaaggaata ctcccaacaa ttttatacct ctgtttgtta gggaattgaa
      601 actgtagaat atcttggtga attaaagtga cacgaatgtt cagttttaat ttttctgacg
      661 ataagttgaa tagatgactg tctaattcaa tagacgttac ctgtttactt attttagcca
      721 gtttcgtcgt taaatgccct ttacctgttc caatttcgta aacggtatcg gtttctttta
      781 aattcaattg ttttattatt tggttgagta ctttttcact cgttaaaaag ttttgagaat
      841 attttatatt tttgttcatg taatcactcc tgaagttatt acatctgtaa ataaatacag
      901 aagttaaacg atttgtttgt aattttagtt atctgtttaa aaagtcataa gattagtcac
      961 tggtaggaat taatctaacg tatttattta tctgcgtaat cactgttttt agtctgtttc
     1021 aaaacagtag atgttttatc tacattacgc atttggaata ccaacatgac gaatccctcc
     1081 ttcttaatta caaattttta gcatctaatt taacttcaat tcctattata cacaaaa
//
LOCUS       DACEUZ010000233         1091 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.245, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000233 DACEUZ010000000
VERSION     DACEUZ010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.245"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            6..980
                     /locus_tag="IXG44_002623"
     CDS             6..980
                     /locus_tag="IXG44_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAQ1659310.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGETKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          384 a          135 c          187 g          385 t
ORIGIN      
        1 aaactatgtg tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg
       61 attatgaaat atcttacaat gaagtagtta ctgttactcc aagaaattat aagttgaatt
      121 ttcgaaaggt aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag
      181 ctgactaccc tctttattac gatgcgacaa atgaaaaagg attgagtatg gctgggctaa
      241 atttttctgg gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt
      301 ttgaatttat tccttggatt ttaggacaat gctcaacagt aggagaaact aaaaaattgt
      361 taaaaaatat caatttagca aatataaatt atagtgatga acttccttta tcccctttac
      421 attggctatt agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc
      481 atatatatga taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat
      541 ttaatttaaa caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc
      601 aaataagttt gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat
      661 cctcagtatc tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt
      721 cagagtcaga aagtattagt caatttttcc atatcttagg ttcagtagaa caacaaaaag
      781 gtttgtgtga tgttggtgat ggaaaatatg aatatacaat ttattcttct tgttgcaatg
      841 ttgacaaagg aatctattat tatcgaacat atgaagacag tcaaattact gcaattgata
      901 tgaataaaga agacttagat agtcataagt taattagtta tccaattata gaaaaacaac
      961 aaattaaata tataaattag ttaatgtgtt gtgattgatt atttaatata gatataatac
     1021 aaaaaggcaa agatattttc aaaacatttt ttcttgatgt gatatccttg tcttttttgt
     1081 aatgttttta a
//
LOCUS       DACEUZ010000234         1089 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.246, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000234 DACEUZ010000000
VERSION     DACEUZ010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1089
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.246"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            414..947
                     /locus_tag="IXG44_002624"
     CDS             414..947
                     /locus_tag="IXG44_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HAQ1659311.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
BASE COUNT          350 a          203 c          200 g          336 t
ORIGIN      
        1 cttattaccg cagatgcaag cttttgaaca ccgtttattg agaagttgtg aaatcagcgt
       61 cctgacctga gtattagaga aacaaatggc aaaatagttc ctcatatttt gctatttttt
      121 gagctaatac cgagggtcag ctgattgaac accgtttagt ggagaagttg tgaaaaagtc
      181 gcctactttc gagtcgtaag aaattttttt gaaaatggtt cctcacaact ttgcataaat
      241 ttgacttaat accgagaagt catgatcccc atctaactcc aaatcacaag gaatgagttc
      301 aaaaactagc ttttctcaac aaatattaca taaaaaattc tatgtttttt gttaaattta
      361 tttgcttttt tcaaaagtat gttacagtaa tttcccaagg aggttttaca gaaatgaatt
      421 ttgttgccat ggattttgaa actgccaatc atcagcctta tagtgcttgt tctcttgcgc
      481 tagtcatggt aaaaaatagc cagatcgttg atgaattcta tacattgata cagcctgaga
      541 ctcctttttt ctggcgaaat gtccaaattc acggaatcca tcaagaagac gtacgcaatg
      601 cgccgaaatt cccagaagtc tggcaaacta tccaaccata ttttcaaaaa aatcgtttag
      661 tcgttgcaca taatgctgct tttgatacaa aggtcttagc gggatgctta gattattatc
      721 atcttgaaca gcctaattat ctttctttat gtacagtgaa aagtagccgt cgcctgtttc
      781 ctgaatttcc aaatcatcga ttgaacactg tgtgcgaaaa tttgaacatc caattagatc
      841 atcaccatga tgcattggaa gatagtcgtg catgtgcgga aatcctgctt tatcaagaaa
      901 aaaaattcgg gacagatcca ttgaaaaaac ttgtgactat aaagtaaaag aatacttatg
      961 aaacacacaa agaggtcgtg agcctgccgt ttcactccga gtcacaagga gcaatttcaa
     1021 aaaatagctt ctcatgttta ttaaatattg tgactggtga ccgaggagct ggcaggagaa
     1081 caccgttta
//
LOCUS       DACEUZ010000235         1082 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.247, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000235 DACEUZ010000000
VERSION     DACEUZ010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1082
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.247"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            700..>1082
                     /locus_tag="IXG44_002625"
     CDS             700..>1082
                     /locus_tag="IXG44_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004853597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAQ1659312.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMW"
BASE COUNT          413 a          161 c          244 g          264 t
ORIGIN      
        1 aaaagctaag gcactaataa aagaacgaaa aagtttggtt attaggagaa ctgataatct
       61 gacaggtgga atgatgaagt aacgctctga aacgcctgcc ctgagcctcc gacaagcatt
      121 gtatgaggaa actcagaatg tttgaagctc ggtaaagacg gctgaaagat accgtaatag
      181 ttgatgaaca tatatcttct atcgaaaact atccagaggt gggtagcgta tccaacaggt
      241 cgggggtcta taaaatatct atggttagaa tgttctataa ggaactgacg aaacagcgaa
      301 tgtacgggtc taaatttgaa ctttgtagaa atgcaaaggt acgagaaatc gtagctggtg
      361 tggcaaagta agaatatagg ttatgaaatg ccatataacg ttacaggcgg tatcaagcca
      421 gcaggcttat agctagtgac ctaaggatat atatgtatag ataagattat cggaacgagg
      481 aaaggtattg ggttctcatc aagagaataa actgacgaag aacaataagc agttgaacta
      541 atgctgaaaa gcagagggcg aacctaagaa attcttgtaa tgagaatgga ggaatgccct
      601 caagtcgtgt atatattaga aaagtattat gttgtttcaa taaggattac gagtaagata
      661 aaaaagctca ctcaatttca agtaaaggag tgaagcctta tgcctaaaaa taaaaatgaa
      721 acattgtgtg tagaagattt aagacacgct gaatattacg aaatgcaaaa cacctttgat
      781 gatttatatg ccaaaagtaa gaatggagat attttcactc atcttatgga tattatccta
      841 tcgagagaaa atatattact ggcatatcga aatatcaaag caaatgcagg cagtaaaaca
      901 gcaggaacag acggtacaat aattaaagat attggcaaat tgccagcaga aacagttgtc
      961 aaaaaagtaa gatacattgt tgcaggcagt ccacatgggt atcgacctaa gccagtgagg
     1021 cgaaaagaaa taccaaaacc aaatggtaaa accagacctc taggtattcc ttgtatgtgg
     1081 ga
//
LOCUS       DACEUZ010000236         1035 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.249, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000236 DACEUZ010000000
VERSION     DACEUZ010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.249"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(<1..>1035)
                     /locus_tag="IXG44_002626"
     rRNA            complement(<1..>1035)
                     /locus_tag="IXG44_002626"
                     /product="16S ribosomal RNA"
BASE COUNT          218 a          324 c          234 g          259 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      961 cttggtgagc cgttacctca ccaactagct aatgcaccgc gggtccatcc atcagcgaca
     1021 cccgaaagcg ccttt
//
LOCUS       DACEUZ010000237         1003 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.250, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000237 DACEUZ010000000
VERSION     DACEUZ010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.250"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            <1..>1003
                     /locus_tag="IXG44_002627"
     CDS             <1..>1003
                     /locus_tag="IXG44_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1659313.1"
                     /translation="GIKTSEALYKNTAEIHMQYKDDMDFNSYINALYEISTQENINIS
                     QYSFTAVNQLTILATNPNANKHWKFSKKNILARNDSKVHYTNNKNSKYQLKLPNNFLN
                     INIYPFSHVENIGLSEILYVQGNSNKLIPVLKKYGTPTISNINQSDTFQINTNIVLVI
                     CYLSIFIVITTIIFAFSKMKEITLKKMLGYNSFDTIKDSIKELNLLFCSLIITFILSI
                     FLSIYYSNENFFLSFLLCSIFHFIISFLFIIVTLSIYHLSSIKNYLKNSRPLKLNLCI
                     LNICLFLSMILLLIASTKVINTHNEAENNSLKYWERTTNLYKTNITNQLNRNNTVEEN
                     "
BASE COUNT          397 a          144 c           92 g          370 t
ORIGIN      
        1 ggtataaaga cttctgaagc gctatataaa aataccgctg aaattcatat gcaatataaa
       61 gatgatatgg actttaacag ttatataaac gctttgtatg aaatatctac acaagaaaat
      121 attaatattt ctcaatatag ttttactgct gttaaccaac taactattct tgctacaaac
      181 cctaacgcta acaaacactg gaaattttct aaaaaaaata tattagcaag aaacgattct
      241 aaagtccatt atactaataa caaaaattct aagtaccaat taaaacttcc aaataatttt
      301 ttaaatataa atatttatcc tttttctcat gtggaaaata ttggattaag tgaaatatta
      361 tatgtacaag gaaactcaaa caaattaatt cctgtactga aaaaatatgg aacacctact
      421 atttccaata tcaatcaatc agatactttt caaattaata ctaatattgt tcttgttatc
      481 tgctatctta gtatatttat tgtaattact acaattatat ttgctttttc aaaaatgaaa
      541 gaaatcactc taaaaaaaat gttaggctat aactcttttg atactataaa agattctatt
      601 aaagagttaa acctcttatt ttgttctttg attataactt tcattttatc tatttttttg
      661 agcatatatt acagtaacga aaattttttc ctatcatttc ttctatgtag tatttttcat
      721 tttataatta gttttttatt tatcatagtt actctaagta tctatcattt atctagtata
      781 aaaaattatc taaaaaattc acgaccactt aagttaaatc tttgtatatt aaatatctgt
      841 ctattcttaa gcatgatcct tctgttaata gcttctacaa aagtaataaa tacccataat
      901 gaagccgaaa ataattctct taaatattgg gaaagaacta ctaatttata caaaacaaat
      961 attactaatc aattaaatag aaacaacacc gtagaagaaa ata
//
LOCUS       DACEUZ010000238          998 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.252, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000238 DACEUZ010000000
VERSION     DACEUZ010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..998
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.252"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(81..635)
                     /locus_tag="IXG44_002628"
     CDS             complement(81..635)
                     /locus_tag="IXG44_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ1659314.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADVAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGG"
BASE COUNT          323 a          185 c          107 g          383 t
ORIGIN      
        1 cttccgaaaa ttcagttatc ttcattaatc tcaccttcta agttttcatt atacacaaaa
       61 aatatatcct aatatctcaa ttagcctccc aatttaaaac cacgtaacga tgcccctatt
      121 tcatcttcag ttataccaat gtaccttttt gtaattgctt gcgagctatg attaaaaata
      181 aacattaagg tagcaatgtc tctagttttc ttgtagtagt gatacccaaa agtttttctt
      241 aatgtatgtg ttcctatatc atctcttcct aatttttctg ctacatctgc atatatctga
      301 tagacacgat cccttgttat atgtcgacct tgttgccgac ttggaaataa ccaatcattt
      361 tcttctttac cttcagtata taataaaatc tcttctctca aattatctat aaaaatcggt
      421 tttcgcttac ctgttttatg ttcaacaata ataggcttac ttttacctcg tatgtctcca
      481 acttttaatc ctataatatc tgacatacgt aacccattat tgattccaaa caaaaataaa
      541 aaactatctc tttctggatt acttgttctt cttagccaaa actgaaaatc ttgtatctct
      601 tcatttgtcc ttaacggttg tacattatag ctcactattg tttcccttct taaaaatatt
      661 ctgagttcat gattatatat atattttacc ataatcatga acttatatat atactctgtt
      721 aaaagtgaag aaaatcaagg ggtttagttt atgattttat atcatttcag taactcagaa
      781 tattccgact tttttgtaca aaaaaattaa ccttacttat atgaagattc taagctctta
      841 tttaaatttt agacgggcag ttatccgtag aagttcttag aatagctaat ttacataatt
      901 aaattatatg aaatttaagt ttgaacaata tactaagaac tccccttact ataaagtgaa
      961 ataaattagc atatgaataa ccgtgctacc aaactttt
//
LOCUS       DACEUZ010000239          987 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.253, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000239 DACEUZ010000000
VERSION     DACEUZ010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..987
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.253"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            <1..566
                     /locus_tag="IXG44_002629"
     CDS             <1..566
                     /locus_tag="IXG44_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1659315.1"
                     /translation="EQLYLESETSQTKVTVSHNRLDVYKRRPETVKKGTELLRLETEK
                     YIMNVYLERSELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKDATIDD
                     YYLVFTRFLTCGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYT
                     YDDTEEIQNELDRKRMPYQRFERKKRQ"
BASE COUNT          340 a          168 c          212 g          267 t
ORIGIN      
        1 aagaacaatt gtatttggaa agtgaaacga gtcaaacgaa ggtcacagtc agccataatc
       61 gtttagacgt gtataaacgt cgtcctgaaa cggtaaaaaa aggaacggag cttctacgac
      121 tagaaacaga aaaatacatc atgaatgttt acctagaaag gagtgagctg gtcgaaaact
      181 ttgtcagcta cacgatttgc aggaaacact attactcaga atcagaatat tattcaattt
      241 ttgagagcat ggaattaatg gaagaagaaa tggaagagct aaacagcaaa gatgcgacga
      301 tagacgatta ttaccttgta tttactagat ttttgacgtg tggaacggaa tctatcgtgt
      361 taatgggaat ggattatgaa ctcgctgtac ccgaagcatt tttgagggaa tatgtagaag
      421 aagtagataa cccaggcgac gactggtaca cgactttaac tgatttttta gacacctata
      481 cttacgacga tacagaagaa atccaaaatg aacttgaccg taaacgaatg ccatatcaac
      541 gatttgaaag gaaaaaaagg caataatcat ttataccgaa aagtgagaat gtgtgaaata
      601 aggacaaaaa acctagtaac taagggaatg gttcttattt cgcaccaatc gctccaaccg
      661 cccccgccgc ttaggtggtg cgaaataagc gggcaacacc cgataaccta agagaaagaa
      721 ggagatccat atgaataaaa tcattcctct attgttagtc ctatttgttg gcgaaatact
      781 tatgcgccat gctgcagctg tgcatttaaa cttcgtagta tttttggcaa tactggtatt
      841 ttgtttgatc ttttggcgct ttgtattagc ccttgtgagt ctactattca agatgattgc
      901 agccatattt ttcatagggt tatttcgatg aaaggagaga gtactaatgc caattgcaat
      961 aggaaataaa cggttacccg ttacgtt
//
LOCUS       DACEUZ010000240          929 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.254, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000240 DACEUZ010000000
VERSION     DACEUZ010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..929
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.254"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(57..>929)
                     /gene="menA"
                     /locus_tag="IXG44_002630"
     CDS             complement(57..>929)
                     /gene="menA"
                     /locus_tag="IXG44_002630"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HAQ1659316.1"
                     /translation="FSIAFFHEIHWLNTFLFFIGMLVFDLATTAINNYMDYEKAHSEV
                     YKYEENVIGRRNLSPVLIRNMILAMIAFVFVIGVILTINTGWLLLLMGFICCFIGVFY
                     TFGPIPLSRMPLGEIFSGVTMGLGIFAMVVYVNTFGSDVYDLTVNFSSGQFDLVGNLW
                     AIAAIVLASLPLVFTIANIMLANNLRDLDRDIENHRYTLVFYIGRPAGTVLFQLLMYA
                     CYFVILMGLLSGIYEWPILVTFLTLPIVKKHLDQFKQTLPQPISFSYSIKNMVLFNAS
                     YVLGLLLTIIMQAI"
BASE COUNT          340 a          185 c          161 g          243 t
ORIGIN      
        1 tttggctgtt cttattactt gatgcagggc agttttttca caacctcttc atgctgttaa
       61 atggcttgca taatgattgt tagtaatagc cccaatacat aactggcatt gaataagacc
      121 atatttttga tggaatagct gaaactgata ggctggggta acgtttgctt gaattgatcc
      181 aaatgcttct tcacaattgg caaggtcaag aaggtgacca agatcggcca ttcatagata
      241 ccgctcaaca accccattaa aatcacgaaa tagcaagcat acatcaatag ctggaataag
      301 actgttcccg caggacgtcc gatataaaaa actagtgtat agcgatgatt ttcaatatca
      361 cggtcaagat cccgcaaatt attagccaac atgatattgg caatcgtaaa caccaagggt
      421 aaggaagcta atacgattgc tgctatagcc caaagatttc ctaccaaatc aaattgtcca
      481 gatgaaaagt tgactgtcag atcatagaca tctgaaccga atgtattgac ataaacgacc
      541 atggcaaaaa ttccaaggcc catagtcacc ccactgaaaa tttcccctaa aggcattcga
      601 gataaaggaa tcggtccgaa tgtatagaat actccaataa aacaacagat aaatcccatc
      661 aaaagtaaaa gccaacccgt attgatcgtt agaataacgc cgataacaaa aacaaatgca
      721 atcatagcta aaatcatgtt tcgaatcaat acaggagata gatttctgcg gccaatgaca
      781 ttttcttcat acttatatac ttcagaatga gccttttcat aatccatgta attattgata
      841 gcggtagtcg ctaaatcaaa aactagcatt ccaataaaaa ataaaaaagt gtttagccaa
      901 tgtatctcat gaaaaaaagc gatagagaa
//
LOCUS       DACEUZ010000241          927 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.255, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000241 DACEUZ010000000
VERSION     DACEUZ010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..927
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.255"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            55..315
                     /locus_tag="IXG44_002631"
     CDS             55..315
                     /locus_tag="IXG44_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1659317.1"
                     /translation="MKKIDLINMIGMLIGILVNIVIFTDWLGVLFSNLIPILIIGICG
                     IILSILELFESRNTMNRIFACIILIVNLLPMVYFTFLYFALG"
     gene            complement(589..>927)
                     /gene="tnpB"
                     /locus_tag="IXG44_002632"
     CDS             complement(589..>927)
                     /gene="tnpB"
                     /locus_tag="IXG44_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAQ1659318.1"
                     /translation="IKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKAD
                     WYGRKIIKVDKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTE
                     GLRLHSMGLA"
BASE COUNT          301 a          147 c          144 g          335 t
ORIGIN      
        1 actctcaatt attttaatat aagtacttca gatttaataa aggagaaata tctaatgaaa
       61 aaaattgatt taataaatat gataggtatg ttaataggga tattagtaaa tatagtaatt
      121 ttcacagatt ggctcggggt gttattttct aatcttattc ccatacttat tataggcatt
      181 tgtggaatta tactttcaat attagaactt tttgaaagca gaaatacaat gaatagaata
      241 tttgcttgta ttatattaat tgttaatcta ttgcctatgg tctattttac ttttttatat
      301 tttgcactgg gatgaaataa attaaccaaa agtttctttt tcttataatg tgtatcaata
      361 ttaaaactga aaaagatatg gataatagga tcaaagccct cattggaaca ctcaatatat
      421 tatcagatgt gaactactca ccacttagct aaacctgacg gttcttaacg cttgaagtgg
      481 gagcttcttg ggaatagagc atacttgata gcgtatttct ttactaacag cggtgtctct
      541 tatttaccaa gctatccccg tagttcctac ggttcttgat gattggatct aagccaatcc
      601 catagaatgt agtcttagac cttcggtcag aatattgata ctagcattga tatcacgatc
      661 atgatgagta tgacaaacag gacaagtcca ttctcgaatt tcaagagatt tcttgccatc
      721 tttatgtccg cactctgaac aaatctgact agacggaaac catttatcta ctttgatgat
      781 ttttcgtcca taccagtcag ccttgtattg taatttagtc acaaactttg accaagaaac
      841 atcagaaata cttttagcta atttatgatt tcgcaacata ccttttgtgt ttaagtcttc
      901 aatacagata atatcgtgat ttttgat
//
LOCUS       DACEUZ010000242          901 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.256, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000242 DACEUZ010000000
VERSION     DACEUZ010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..901
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.256"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            <1..>901
                     /locus_tag="IXG44_002633"
     CDS             <1..>901
                     /locus_tag="IXG44_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid biosynthesis protein F"
                     /protein_id="HAQ1659319.1"
                     /translation="LPIQKNLIVFSSFGGRNYSGNPRVISEKIHEKKLNYRIIWLIKK
                     EAIITDLPDYIKRIDSDSFLAAFYLYTAKYWIDDSRKTIKYAKRKKQLYFQTWHGTPL
                     KKIEFDAKDKLPKRYLSYAKKDSESITYLLSGNRYSSKKYVSAFNIVPSKILEVGTPR
                     NDELASSKEQVFDLKKKQINVLFAPTFRNNIEDNGITQLEWLGVDNLREFFKKQQQEL
                     NFMTKFHPNVHSKLATDSKSIEYMKKHQITLINDGIPLEMLFEEIDVLITDYSSIFFD
                     FALTKKPIILFNYDEKEYSLERGF"
BASE COUNT          349 a          116 c          138 g          298 t
ORIGIN      
        1 ttacctattc aaaaaaattt aattgtattt tcaagttttg gcgggcgtaa ctattctgga
       61 aatccaagag ttatttcaga gaaaattcat gaaaaaaaat tgaattacag aattatttgg
      121 ttaatcaaaa aagaagcgat tataactgat ttaccagatt atattaaaag aattgactcc
      181 gattcttttt tagctgcctt ttatttatat acagctaagt attggataga cgatagtcgg
      241 aaaacgatta agtatgcaaa aaggaaaaaa caactttatt ttcaaacatg gcacggaacg
      301 cctttaaaaa aaattgaatt tgatgcaaaa gataagttac ctaaacgtta cctaagctat
      361 gcgaaaaaag atagtgagtc aattacatat ttattatctg gtaatcgcta tagttctaag
      421 aagtatgttt cagcattcaa tatcgttcct tctaaaatat tagaagtagg aactcctaga
      481 aacgatgaat tagcctctag taaagagcaa gtgtttgact tgaaaaagaa acaaatcaat
      541 gtcttatttg ctccaacttt tcgaaataat attgaggaca acggaattac gcaactagaa
      601 tggttaggtg tggataactt acgtgaattt tttaaaaagc aacaacaaga attaaatttt
      661 atgactaaat ttcatcctaa tgttcatagt aaattagcaa ctgattctaa aagtatagaa
      721 tatatgaaaa aacatcaaat tactttaatt aatgatggga ttcctcttga aatgcttttt
      781 gaagagatag atgttttaat cacagattat tcatctattt ttttcgattt tgcgctaaca
      841 aaaaaaccaa ttattctttt taactatgat gaaaaagaat atagcttaga aagaggtttt
      901 t
//
LOCUS       DACEUZ010000243          895 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.258, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000243 DACEUZ010000000
VERSION     DACEUZ010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..895
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.258"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            <1..618
                     /locus_tag="IXG44_002634"
     CDS             <1..618
                     /locus_tag="IXG44_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAQ1659320.1"
                     /translation="PFYKEEESLMPYFALAAQRAGAVGIRANSVRDIIEIRKCVSLPM
                     IGIIKRDYPPESPFITATMREVDELVETGVEVIALDCTLRNRHDGLSINSFITKIKEK
                     YPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGYTEESKKQDGPDIDLIKKLVEEGIC
                     VIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQEIASRFVKALS"
     gene            632..>895
                     /locus_tag="IXG44_002635"
     CDS             632..>895
                     /locus_tag="IXG44_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1659321.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAG"
BASE COUNT          313 a          116 c          194 g          272 t
ORIGIN      
        1 ccattttaca aagaggaaga aagtttaatg ccttattttg ctttggctgc tcaaagggct
       61 ggagcagttg gtatacgtgc aaattctgtt cgagatatta ttgaaattag aaagtgtgta
      121 tcattaccaa tgattggaat tattaaaaga gattatccac cagaatctcc attcattact
      181 gccactatga gagaagtaga tgaattagta gaaaccggtg ttgaggtaat cgctcttgat
      241 tgcactctta gaaatcgaca tgatggacta tctataaatt cttttattac taaaataaaa
      301 gaaaaatatc ctcatcagtt atttatggca gatatttcta catttgaaga aggcaaaaat
      361 gcatataaag caggaataga ttttgtagga actactttaa gtggatatac tgaggaaagt
      421 aaaaaacaag atgggccaga tatagatttg attaaaaaat tggtagaaga agggatttgt
      481 gtaattgctg agggaaggat tcatactcca gaacaagcaa aagagataaa aaaaatagga
      541 gttgcaggta ttgttgtggg tggtgctatt actcgaccac aggaaattgc atcaagattt
      601 gttaaagcat taagttaggg ggagaaataa aatgtttaaa aaattttcac aattaggacg
      661 cgctttcatg ttaccaattg ctatattacc agtagccgga ttactattgg gactgggagg
      721 agcgttaaca aatgagagtg ctataaatgc ttatccattt ttagatcaac cgtggttgca
      781 tacaatttta agtattatga gttatgctgg aaatgcagtt tttgcaaacc tagcactaat
      841 ttttgcaatt ggtattgctg taggacttgc aaatggagat aaaggaacag ctggt
//
LOCUS       DACEUZ010000244          862 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.260, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000244 DACEUZ010000000
VERSION     DACEUZ010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..862
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.260"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(<1..434)
                     /locus_tag="IXG44_002636"
     CDS             complement(<1..434)
                     /locus_tag="IXG44_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1659322.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHR"
     gene            complement(451..855)
                     /locus_tag="IXG44_002637"
     CDS             complement(451..855)
                     /locus_tag="IXG44_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HAQ1659323.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKE
                     MFWSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
BASE COUNT          260 a          139 c          136 g          327 t
ORIGIN      
        1 tgtctatgaa gcttaattct tactaatgac ttcaatttag gaactttgat gaatttacta
       61 tcaatcaaag caactgtacc attttgatta ttcgttgtat aactctgaac aggatttttc
      121 ttacttttga aacgtggaaa tccaacggat ttatcccgaa aaaaattctt gtatgcttta
      181 tctaaattaa gttgggcatt ggctaaagca aggctatcaa tttctttcaa aaacggaaat
      241 tctttcttgt atttagctgg tgtcggaaaa gtcatttttt tagaagaatc atttttaact
      301 tcttcatacg ccttttttcg gtcatcaagc attagattgt agaccttacg gacacaacca
      361 aaagatttgg caaagaagat ttcttgttcc tctgttggat agattctgaa tttgtatgct
      421 tttagttgtt ccataaagtt cacctacttt ctatttatga ttgtttcctt gattttggat
      481 atacttctta atcacatcaa ttggtgcgcc accagttgtc agaagacaaa aacttttaga
      541 ccagaacatt tctttccaaa gaaattgttt gactcttgga aagtcacgtt ttatcaatct
      601 tgagctggca cttttatagg cattgatgaa ttttgtcaat tctgttttag gttgagcttt
      661 gaacataata tgaacgtggt ctttatcatg attccactcc actaaagtaa tgtgatacga
      721 ttctgaaatt ctttcaaaag tagttttggc ataatcagat atttcatcat caatcacttg
      781 tctacgatat ttcgttacta aaacaaggtg gtaataaaga agaaatactg aatgattatt
      841 tgtatctaat tccatgttat at
//
LOCUS       DACEUZ010000245          860 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.261, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000245 DACEUZ010000000
VERSION     DACEUZ010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..860
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.261"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            <1..856
                     /locus_tag="IXG44_002638"
     CDS             <1..856
                     /locus_tag="IXG44_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAQ1659324.1"
                     /translation="KRFYRPLNPFKQTLPKVLCFDEFKSVRSVASAMSFIMMDGQSHA
                     LLDIVENRRLPYLERYFSRFSLATREAVQLIVIDMYAPYVSLVKKLFPNAQLIIDRFH
                     IVQHIGRTFLNHRVKETTVRLKGNSHSQRGLGKKLKRYWKLLQKEEAKLNYEKRVWRA
                     SFKDYLTESEIADRLLAACPNLRQGYQLYQDILYAVKKRDQSLFEDCLTRDISGLPET
                     YTTTLRTFKKFLPQIQNALHYSYSNGPLECLNNHIKVLKRNAYGFRNFYNFKLRIMIR
                     HGKTFLTK"
BASE COUNT          273 a          173 c          165 g          249 t
ORIGIN      
        1 gaagcgattc taccgcccat tgaatccttt caaacagact ttgccaaagg tcttatgctt
       61 tgatgaattc aaatcggttc gaagtgttgc cagtgcaatg agttttatca tgatggacgg
      121 acaaagccat gcattattgg atattgtcga aaatcgacgc ttgccttatt tagaacgcta
      181 tttttctcgg ttttcattag cgactcgtga agccgttcaa ttgattgtta ttgatatgta
      241 tgcgccttat gtttcgttgg tgaaaaagct tttcccgaat gctcagttga tcatcgatcg
      301 tttccacatc gtccaacata ttggcagaac tttcttgaat catcgagtga aagaaaccac
      361 ggtacgactt aaaggaaatt cccactctca acgagggtta gggaaaaaat tgaagcgcta
      421 ttggaaactc ctacaaaaag aagaagcaaa gttaaactat gaaaaacggg tttggcgtgc
      481 cagttttaaa gattatttga ctgaaagtga gattgccgat cgactgttag ctgcttgtcc
      541 caacttacgc caaggctatc agttatacca agacattctt tatgcggtaa agaaaagaga
      601 tcagtcacta tttgaggatt gtttaactag agacattagc ggtctgccag aaacctatac
      661 aaccacttta cgaacgttta aaaagtttct gccacagatc caaaatgcgc ttcactacag
      721 ctattcaaac ggaccattgg aatgcttaaa caaccacatc aaagtattaa aacgcaatgc
      781 gtacggcttt cgcaactttt acaatttcaa actacgaatt atgattcgtc atggcaaaac
      841 atttttaacc aaataaacag
//
LOCUS       DACEUZ010000246          856 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.262, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000246 DACEUZ010000000
VERSION     DACEUZ010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..856
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.262"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
BASE COUNT          328 a          101 c          109 g          318 t
ORIGIN      
        1 attagcaata tataaaagaa ggtatcaaga gtcacagtga atttattccg aaaagtgaaa
       61 agagcaaata taaaatttaa taaaaataag aactaaatag gaaaacgtag acgtaatttt
      121 ctacgttttc cttttcatat atattaaata ttacggaaaa ttatataaac taatttagtg
      181 ttttgtttgt aattcctaat tttgctccac ttttagataa tttttaggtc tatttaactc
      241 attagacatt ctatgctcat ttttctccaa atttgtttca actctttcgt acttaatttt
      301 gcgtatttga aaattcttct tttagagtac ttttattacg atactagtat gatatatatc
      361 tacagcaaac agttaaaatc ttcctaaaat aagcgaatag cattcctaaa acctataaaa
      421 tttttagatg aagcgacaag tggacaatta ataaactact tgaacatgaa attatggatc
      481 acctttcaaa tttagaaaat ggaattctta tacttattgc gcaacattta ttgattgcaa
      541 aattatgtga caaaaagaac tagtagaaga aggaataatg gcttgataat aaagtactac
      601 ctattaaagg tagtacttta tttaatttag tataaattaa catactacta tattttaggt
      661 ttcatattat ataaattaat aatatactac catttgttgg tagtataatt taaaggtaat
      721 aagttattct tttattacta tgtttgggtt ttatagcaaa gaaagtttaa taagagtttg
      781 gacctagcaa acaatattaa tgaggtcatt aaatcttatt atctttagtg atataatgaa
      841 atatctacac gacaaa
//
LOCUS       DACEUZ010000247          790 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.265, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000247 DACEUZ010000000
VERSION     DACEUZ010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..790
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.265"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            <1..781
                     /locus_tag="IXG44_002639"
     CDS             <1..781
                     /locus_tag="IXG44_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1659325.1"
                     /translation="DMLDYYYFEKNLSYNYVIHLKTFTGMIFKFANRRYGLKYNPVNG
                     VSLARKPKTNEEIKKEKDGYLSKEEVQMIASKQKSSHQQSRYSLITKFLFLTGLRYGE
                     LISLTESDYDGNKITVSGTYDYELKIKTTTKNTGSYRTIELSSNAKEIIDQLIEENKL
                     IKNNKDKYIFISKNGNPISIQAYNQSLRAVSKELNIDKKVSSHMLRHSHISLLTELGI
                     PLKAIMDRVGHEDSKTTLKIYTHTTKNMQNQLVEKLGKIEI"
BASE COUNT          339 a          107 c          102 g          242 t
ORIGIN      
        1 agatatgcta gattactact attttgaaaa aaatttatca tataattatg taatacattt
       61 aaaaactttt acaggaatga tttttaaatt cgccaaccgt cgatatggat taaaatataa
      121 tcccgtaaat ggggtttctt tagcaagaaa acctaagaca aacgaagaaa taaaaaaaga
      181 aaaagatggt tatttatcaa aagaagaagt tcaaatgatt gctagtaaac aaaaaagttc
      241 acatcaacag tcacgttatt ctttaattac taaatttttg tttttaactg gactacgtta
      301 tggtgaattg atatctttga ctgaatctga ttatgatggt aataaaatta cggtttctgg
      361 tacttatgat tacgagttaa aaattaaaac aacaactaaa aatacaggtt cttatagaac
      421 cattgaatta tcaagtaatg ctaaagaaat catagatcaa ttaattgaag aaaacaaatt
      481 aattaagaat aataaagata aatacatttt catctctaaa aatggtaacc cgatatctat
      541 tcaagcatat aatcaatctt taagagcagt atccaaagaa ttaaacatag ataaaaaagt
      601 ttcttctcac atgttaaggc attcccacat ttctctactt acagaattag gaatcccatt
      661 aaaagccatt atggatcgtg taggacatga agattcaaaa acaacattaa agatttatac
      721 tcatacaact aaaaatatgc aaaatcaact agtagaaaaa ctaggaaaaa tagaaattta
      781 aattgcccct
//
LOCUS       DACEUZ010000248          751 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.266, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000248 DACEUZ010000000
VERSION     DACEUZ010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..751
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.266"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            36..>751
                     /locus_tag="IXG44_002640"
     CDS             36..>751
                     /locus_tag="IXG44_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ1659326.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLY"
BASE COUNT          280 a           96 c          123 g          252 t
ORIGIN      
        1 taatggagaa aagttgaaat tgtagaggag tttgtatgag ttatatctat ttaattaaag
       61 aaaattatcc atttttaacg aagtctgaaa aaaaagtcgc tgattttatt ttaaattctg
      121 ggaaaactat tatttatagt acgatgagtg atattaagaa aaacacaaaa gttggcgatg
      181 ctactattat tcgcttttgt caaaaaattg gtttttctgg tttttctgat ttaaaaattg
      241 aaattgcaaa ggaagacttt agtcaaaaaa aagaacatcc gactagtgta aagtactatg
      301 atgaaattgc taatagttta acagaagctc tctattccac tattaggctt ttagacgaag
      361 aaaaattgaa tgaagccatt aaaatgatca ctcaatcaaa aaacatatat atatttggag
      421 tgggatctag tggaaatact agtcttgatt tagaaaatat gtttcttcgc gtaggagttc
      481 aagcaaaagc tgtactagac cctcattttc aagcccaagt cgcttcttta ttaacagtta
      541 atgatttagt aatcattttt tctttatctg ggaaaacgaa agatacttat gattcattga
      601 aaatagctaa aaaaaatggc gcaaaaatta ttgctataac taactatatt cattcaccta
      661 taggaaagtc tgcagactta gttttacaaa cagccattga agaatttctt aatggtggat
      721 ctttagctgg aaaaatatca caactttata t
//
LOCUS       DACEUZ010000249          718 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.267, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000249 DACEUZ010000000
VERSION     DACEUZ010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..718
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.267"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            <1..696
                     /locus_tag="IXG44_002641"
     CDS             <1..696
                     /locus_tag="IXG44_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAQ1659327.1"
                     /translation="QLPFLKRYFSHFSREIREAVEWIVIDMYAPYVSLVKKLFPKAQL
                     IIDRFHIVQHIGRTFRNHRIKETNQLLKSKEQKHYQLGKQLKRYWKLLQKDERKLDYT
                     RRLWRPGFKAHLTETDIVDRLLKGSPALRVGYQLYQDFLYAVKERDYVSFEELLTNNI
                     MLPEGYQTTLRTFQKFLPQIKNALQQSYSNGPLECLNNHIKVLKRNAYGFRSFYNFKL
                     RIMIRHGNALIFN"
BASE COUNT          241 a          146 c          127 g          204 t
ORIGIN      
        1 caactgcctt ttttaaaacg ttattttagt catttttcac gagaaattcg tgaagcggtc
       61 gaatggatcg tgattgatat gtacgctcct tatgtatcgc tcgttaaaaa gctgtttcca
      121 aaagcacagc tgattattga ccggtttcac atcgtccaac atattggtcg tacctttcgc
      181 aatcatcgaa tcaaagaaac gaaccaacta ttaaaaagca aagaacaaaa acactatcaa
      241 ttaggtaagc aattaaaaag atactggaaa cttcttcaaa aagatgaacg caagcttgat
      301 tatactcgac gcctttggcg acctggcttc aaagctcatt taacagagac cgatattgtg
      361 gatcgcttac tgaaagggag ccctgctttg cgtgtaggct atcagttata tcaagacttt
      421 ctttacgctg taaaggaacg agactacgta tcgtttgaag aactacttac caataatatc
      481 atgcttcccg aaggctatca gacgaccttg agaacgttcc agaaatttct gccacaaatc
      541 aaaaatgctt tacaacaaag ttattcaaat ggcccacttg aatgtctgaa caaccatatc
      601 aaagtgttaa aacgaaatgc atatggcttt cgtagctttt ataatttcaa attgcgcatc
      661 atgattcgtc atggcaacgc attgattttc aactaaaaaa gatcaagagg aactgatc
//
LOCUS       DACEUZ010000250          701 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.269, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000250 DACEUZ010000000
VERSION     DACEUZ010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..701
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.269"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            132..>701
                     /locus_tag="IXG44_002642"
     CDS             132..>701
                     /locus_tag="IXG44_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAQ1659328.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSL"
BASE COUNT          222 a          109 c          171 g          199 t
ORIGIN      
        1 tttcttttga aaagcgatta gtatttcaat aaggaataaa aatacttcgt ttaattgaca
       61 atctatgctt gaaagttggc aatgcattcg ttaaactaag tgagattgga aacttgtgag
      121 gaggaaataa tatggcaaat ccaacaattg caatcgttgg ccgcccgaac gtcggtaagt
      181 ctacgatttt taaccgcatc gctggtgagc gtatctcgat tgtcgaagat actcctggag
      241 taactcgtga tcgtatttat gcaaaaggtg aatggttagg tcgagaattc agcgtgatcg
      301 acacaggcgg aattgatctt ggcgatgaac cttttatgga tcagatcaag catcaagcag
      361 aaatcgcgat tgaagaagca gatgtaatca tttgtgtagt cagcggacgc gaaggcgtga
      421 cagacgcaga tgagatggtt gcgaaaattt tatacagaag taataaacca gtgattttag
      481 cagtcaataa agtagataat ccagaaatga gaaacgatat ctatgaattt tattccttag
      541 ggctaggcga tccttatcct gtttcaggaa gccatggact agggattggt gatattttag
      601 atgaagcagt gaagtatttc tctgaagaga ctgaagaaga ggacgacgac acgattaagt
      661 ttagtttgat tggtcgccca aatgttggta aatcctcttt g
//
LOCUS       DACEUZ010000251          690 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.270, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000251 DACEUZ010000000
VERSION     DACEUZ010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..690
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.270"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(52..507)
                     /gene="tnpA"
                     /locus_tag="IXG44_002643"
     CDS             complement(52..507)
                     /gene="tnpA"
                     /locus_tag="IXG44_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HAQ1659329.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
BASE COUNT          189 a          138 c          110 g          253 t
ORIGIN      
        1 ggggattttt aaaagaccgc caactgctca tcttcactca cttacttctc tttatttttt
       61 aaatggttct acgtactctc ttttacttat aatatatcga atgcgatcat cagcttcttg
      121 ttcacggatg tatttttcat ttgtcttttg attcaatccg actgtactca catagtatct
      181 cttcgaccaa aaggatttgt ttccgtattt atatttgaga tttgcgtgct gctcgtggat
      241 tagtaccgca ctacgacctt tcagataccc cataaaactg gatatacata tttttggtgg
      301 tactcgaaca agcatgtgaa tatgatctag cattgcatgt gcttctatga tttccacatc
      361 tttcatttcg cacaacttcc taagtatttt tccaagatct tttcgtaatt gcccatagat
      421 tacttttcgt tgatattttg gtgtaaaaat aatatgatac ttacaattcc aacgagtata
      481 tgctaaactt ttatcgtcat ttgacatgaa aaaactcctt tcgatctgat gagcggttgg
      541 tagccactca ctagtatatc gaaaggagtt tttatcgcta aagatacttc ttccacgcgt
      601 agaaacgcgc ggttttcttg tttcgcacgc tttacgcaca aaactgactg aagtcattaa
      661 gaaaataaaa gggtctaaaa cttttaagtt
//
LOCUS       DACEUZ010000252          684 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.271, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000252 DACEUZ010000000
VERSION     DACEUZ010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..684
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.271"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            1..684
                     /locus_tag="IXG44_002644"
                     /pseudo
     CDS             1..684
                     /locus_tag="IXG44_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
BASE COUNT          251 a          123 c          145 g          165 t
ORIGIN      
        1 taagtatact tttgaactta aattaaagat cgttcatgat tacttggatg gtaaaggcgg
       61 aagtgattat ctggcaaaga aatattccat taaggctcca tcacaagtga agcgctggat
      121 caatgcctat caagaatttg gtgaagaggg tctagtaaga aagcgacaaa agaaaaaata
      181 ttctgttcaa tttaagctgg atgcgataga gttatatcta acaagtgagc tgtcctatcg
      241 tgaagttgcc aacacactta acatgaacaa ccctaatttg atcgctagtt ggatgcgtca
      301 attccgtgag ggtgggatcg atggactctc aaaaacgaaa ggatgtccac ctatcttgtc
      361 taagaaaaat gaacccaaaa ataagaaaaa atctacaact aaagcaacgt ctaaagaacg
      421 tgaacgcatc gaggagttgg agaaacgagt ccgttccctt caaattgaaa atgccttttt
      481 aaaagaattg aggaagctgc gaaaacagga agcccaacaa cgacgaatga agcaatcgca
      541 cgaatcatcg caagcctccg aggaccgttt aaattagttg agcttctaga aactttaaag
      601 ttccctaagg caatctttat gtactggcaa aaacgattgg accgtaaaaa tccagatcaa
      661 gagattgaag cggaaatgct taaa
//
LOCUS       DACEUZ010000253          626 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.272, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000253 DACEUZ010000000
VERSION     DACEUZ010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..626
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.272"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            57..272
                     /locus_tag="IXG44_002645"
     CDS             57..272
                     /locus_tag="IXG44_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HAQ1659330.1"
                     /translation="MKKKLVKGSVICSMIGIGFIVIGTNVEAATYYGNGVYCNKQKCW
                     VNWGQAWSKGVKRWGDNLFGSFSGGRI"
     gene            273..614
                     /locus_tag="IXG44_002646"
     CDS             273..614
                     /locus_tag="IXG44_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAQ1659331.1"
                     /translation="MIDIRETKKIIHELYNSLMKRDKTKAILDITDVLLQVYKKIDSE
                     KYPEILINKLVNYIYIVGFDNKIHFLGNDEKLLIELGDISKKAGINSKYKANFTDKSQ
                     FYSYSEKVPIR"
BASE COUNT          244 a           49 c          121 g          212 t
ORIGIN      
        1 tatataattt ctacatagaa gacgttttct aatttttatt ttcgaggtga atattaatga
       61 aaaagaaatt agttaaaggc tcagttattt gtagcatgat tgggattggt tttatagtaa
      121 taggaacaaa tgtagaagct gccacgtatt acggaaatgg tgtgtattgt aataagcaaa
      181 aatgttgggt aaattgggga caagcttggt caaaaggggt taaacgttgg ggagataatt
      241 tgtttggtag ttttagtgga ggaaggattt aaatgattga tattagggaa acaaaaaaaa
      301 taattcatga attgtataat agtttaatga agcgtgataa gaccaaagct atattagata
      361 ttacagatgt tttgttgcaa gtctataaaa aaatagatag cgaaaaatat ccagaaatat
      421 taataaacaa attggtgaat tatatctata ttgttggatt tgacaataaa attcattttt
      481 taggaaatga tgaaaagtta ttaatagaat tgggagatat atctaaaaag gctggaatta
      541 acagtaaata taaagctaat tttacagata aatctcaatt ctatagttat tctgaaaaag
      601 tacctattag ataattatat ttccct
//
LOCUS       DACEUZ010000254          605 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.273, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000254 DACEUZ010000000
VERSION     DACEUZ010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..605
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.273"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            70..>605
                     /locus_tag="IXG44_002647"
     CDS             70..>605
                     /locus_tag="IXG44_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HAQ1659332.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMDATYIPLKRQTVSKEA"
BASE COUNT          227 a          115 c           98 g          165 t
ORIGIN      
        1 acaaaaaagg aagtcccttc tgtagaataa agttaacgac aaccaattca cagaaaagag
       61 gacttcccta tgaatgattt tactacagaa attgtgcaaa ctctagtcac taaaggcgat
      121 ttaaatgaat tattccgttc gcacttagaa aaagcgataa acacactcct acggactgaa
      181 ttaacggctt ttttagatta cgaaaaatat gatcgcactg gttttaattc aggtaattcg
      241 agaaacggtt cttactttcg atcaatcaaa accgaatatg gtgaattaac attggaaata
      301 cctagagatc gtaatggtga gtttaaacaa caaactttac cagcctacaa aagaacaaac
      361 gatacattgg aaaccactat tatccattta ttcgaaaaag gtgttacgat gtctgaaatt
      421 gctgatttga tcgaaaaaat gtacggtcat cactatactc cacaaaccat gtccaacatg
      481 actaaagttc tgactgaaga agtaaatgcc tttaaatcca gagccttaaa tgataagtat
      541 gtcgctattt ttatggacgc tacttacatt ccactaaaac gtcaaaccgt atccaaagaa
      601 gcgat
//
LOCUS       DACEUZ010000255          593 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.274, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000255 DACEUZ010000000
VERSION     DACEUZ010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..593
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.274"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            <1..>593
                     /locus_tag="IXG44_002648"
     CDS             <1..>593
                     /locus_tag="IXG44_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HAQ1659333.1"
                     /translation="SYAIAPTESTYVWNELLQDINSRGVQEVLLFITDGLKGMKDTIH
                     QIYPKAKYQHCCIHVSRNIAHKVRVKDRKEICDDFKAVYQANSKEEANTFLSGMIEKW
                     KKNYPKVTQSLIENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEE
                     SLERFLVSIFDTYNQKFLNRSHKGFQQVTDTLVSMFT"
BASE COUNT          218 a          111 c           97 g          167 t
ORIGIN      
        1 agttatgcga ttgctccaac tgaatcaaca tacgtttgga atgagctgct acaggatatt
       61 aactccagag gagttcaaga agtcttgctt tttattacgg acggcttaaa aggcatgaaa
      121 gatactatcc atcaaattta tcctaaagca aaatatcagc attgttgtat ccatgtatct
      181 cgtaacatcg ctcataaagt acgtgtcaaa gaccgaaaag aaatctgtga tgactttaag
      241 gctgtttatc aagctaactc aaaagaagaa gcgaatacct tcttatccgg catgattgag
      301 aaatggaaga aaaactatcc taaagtgacg cagtcactca tagaaaacca agacttatta
      361 actttttatg attttccacc tagcattcgt agaaccattt actcaaccaa tctaatcgag
      421 tctttcaata agcaaattaa aagatacagc cgtagaaaag agcagtttca aaatgaagaa
      481 tcactagaac gctttctagt cagcattttt gatacataca atcaaaaatt tctaaacaga
      541 agccataaag gttttcaaca ggtaaccgat acattagttt caatgtttac tga
//
LOCUS       DACEUZ010000256          574 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.275, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000256 DACEUZ010000000
VERSION     DACEUZ010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..574
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.275"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            <1..572
                     /locus_tag="IXG44_002649"
     CDS             <1..572
                     /locus_tag="IXG44_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HAQ1659334.1"
                     /translation="IRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIK
                     GRKIYLSPILDLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQY
                     QMPQYQKKLKENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLN
                     EYIRWYNNKRIKLKLNGLSPVEYRKQSIK"
BASE COUNT          248 a           71 c           94 g          161 t
ORIGIN      
        1 aaatccgaat aaagcgatat aaatcttata aaggaacagt aggaaaaatt gccaagaatg
       61 tgttaaaacg aaatttttca gtagatacac ccaataaaaa atgggtgaca gacgtcactg
      121 aattcaagat aaaaggaaga aaaatctatc tatctcctat acttgattta tttaacggag
      181 aaataattag ttatagtata tcaactagtc caacatataa actgattgaa gaaatgcttc
      241 aacaagcaat taaaaaaaag ggaactgagg gttcactgat tctacattca gatcaaggat
      301 ggcagtatca gatgccacaa tatcaaaaaa aattaaaaga gaataacatt attcaaagca
      361 tgtctagaaa aggaaattgc ttagataatt ctgtaataga aaattttttt ggtgtgctta
      421 aatcagagtt tttttatcga gaaaaattcc gatcaattga gatatttcaa agtaaattaa
      481 atgagtatat taggtggtat aataacaaaa ggataaaact aaagttaaat ggactatctc
      541 cagtagaata tcggaaacag tccattaaat agtt
//
LOCUS       DACEUZ010000257          526 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.276, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000257 DACEUZ010000000
VERSION     DACEUZ010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.276"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(213..443)
                     /locus_tag="IXG44_002650"
     CDS             complement(213..443)
                     /locus_tag="IXG44_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HAQ1659335.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
BASE COUNT          195 a           76 c           78 g          177 t
ORIGIN      
        1 tatatttact tcagatggta aggcaacaaa ataaaataca ataggcagat gaactaataa
       61 ataaaatctg tattaaatac agataaatta atataaaaat caaaaggatc ataaactttt
      121 aacaagtgat gaaatgggtg gaaattgatt cttatagaca gtttgtctca taacattgaa
      181 gtattttgca attttagcat tgaaacactt ttttaatctt caacgttgtt cttataaaca
      241 agatgattgg gaatcttttg aattaacact gtatagctta gaacaccctt tatagaaaac
      301 attcgcttag caatcttaat aattccttgt gcccgatgtc gtttagattc agtaggttcc
      361 tgctcatcga tcatggaaaa aatttgaagg atcagatcct taataaattt atttaagaga
      421 ggatgatcca ttgcttcatc catactcgat aaactagtga taatgagcta cacgtttttc
      481 tttcaagttg atcaatagct tccacgataa aaatatcttt agataa
//
LOCUS       DACEUZ010000258          520 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.277, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000258 DACEUZ010000000
VERSION     DACEUZ010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..520
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.277"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            <1..>520
                     /locus_tag="IXG44_002651"
     rRNA            <1..>520
                     /locus_tag="IXG44_002651"
                     /product="23S ribosomal RNA"
BASE COUNT          149 a          100 c          158 g          113 t
ORIGIN      
        1 agcccgttaa tgggtgatgg cgtgcctttt gtagaatgaa ccggcgagtt acgattgcat
       61 gcgaggttaa gttgaagaga cggagccgca gcgaaagcga gtctgaatag ggcgtttgag
      121 tatgtagtcg tagacccgaa accatgtgat ctacccatgt ccaggttgaa ggtgcggtaa
      181 aacgcactgg aggaccgaac ccacgtacgt tgaaaagtgc ggggatgagg tgtgggtagc
      241 ggagaaattc caaacgaact tggagatagc tggttctctc cgaaatagct ttagggctag
      301 cctcggaatt gagaatgatg gaggtagagc actgtttgga ctaggggccc atctcgggtt
      361 accgaattca gataaactcc gaatgccatt cattcatatc cgggagtcag actgtgagtg
      421 ataagatcca tagtcgaaag ggaaacagcc cagaccacca gctaaggtcc caaaatatat
      481 gttaagtgga aaaggatgtg gggttgcaca gacaactagg
//
LOCUS       DACEUZ010000259          512 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.278, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000259 DACEUZ010000000
VERSION     DACEUZ010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..512
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.278"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            94..>512
                     /locus_tag="IXG44_002652"
     CDS             94..>512
                     /locus_tag="IXG44_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ1659336.1"
                     /translation="MHETGIDAPARNVAANMKNNYNGSNPYTTDVIGDGGIVYRVGEQ
                     GYVSWGAGNANPYAPVQIELQRTYDKALFEKNYRAYIEYTRDSAKKYGIPLTLDQGTS
                     LFTKGIISHLWVTNYVWGNHTDPYGYLSQMGVSKEKLA"
BASE COUNT          175 a           85 c          107 g          145 t
ORIGIN      
        1 cccattaata cgtttgctta tactattaat gacgagtata atttagcgcc gaatcaagga
       61 gactccagat tagcaattcc taacaaaatt attttgcacg aaactggaat agatgcacca
      121 gcaagaaacg tagccgccaa catgaaaaat aattataacg gaagcaatcc ttatactaca
      181 gatgttattg gtgacggtgg gattgtttac cgtgtgggtg agcaaggata tgtttcgtgg
      241 ggagctggta acgctaatcc ttatgcgcct gtacagattg aattacagcg cacatatgat
      301 aaagcattgt ttgaaaaaaa ctatcgagct tatattgaat atacaagaga tagtgcaaaa
      361 aaatatggaa ttccattgac tcttgatcaa ggaacttctt tatttacaaa aggaatcatt
      421 tctcatttgt gggtgacaaa ttatgtttgg gggaaccaca cagatccata tggttactta
      481 tcgcaaatgg gagttagcaa agaaaagctt gc
//
LOCUS       DACEUZ010000260          507 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.279, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000260 DACEUZ010000000
VERSION     DACEUZ010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..507
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.279"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(<1..200)
                     /locus_tag="IXG44_002653"
     CDS             complement(<1..200)
                     /locus_tag="IXG44_002653"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ1659337.1"
                     /translation="MTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKEGISVSL
                     KHVQKLMKQLNLRSIVVKKYRP"
     gene            complement(311..>507)
                     /locus_tag="IXG44_002654"
     CDS             complement(311..>507)
                     /locus_tag="IXG44_002654"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ1659338.1"
                     /translation="SVRGLAKEYGLSEATIYKWKNLYLPDQSTGLTGKEVAELRKENA
                     RLNEELEILKKAAAIFSRKT"
BASE COUNT          151 a           91 c           73 g          192 t
ORIGIN      
        1 tgaggtctat attttttaac tacaatcgac cttaaattta attgtttcat tagcttttgg
       61 acatgtttaa gagatactga aatcccttct tttaataaaa cttgatggat ctttgtggca
      121 ccatagcgtt gtttattcgt aaaaaaaatt gttgaaatct tctttttcaa cttattattt
      181 ctgatttctg tagctgtcaa cggtttattc ttataaaaat aatagacact tctagggatt
      241 tctaataacc gacacaacaa agaaacagtg taatccttac accatttttc aataaattga
      301 acaagcgaat ttaggttttc cgagagaata tggctgcggc tttttttaag atttcaagtt
      361 cctcatttaa acgagcattt tcttttctca gttcagctac ttcttttcca gtcagtcctg
      421 tggactgatc aggtaaatat aaattcttcc atttgtaaat cgttgcttca gataagccat
      481 attctttcgc taaccctcga acagaac
//
LOCUS       DACEUZ010000261          506 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.280, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000261 DACEUZ010000000
VERSION     DACEUZ010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..506
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.280"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            4..>506
                     /locus_tag="IXG44_002655"
     CDS             4..>506
                     /locus_tag="IXG44_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ1659339.1"
                     /translation="MNGFAYTINNEFNLGANEGSSQVANNQYILLHETANETATGRNE
                     AQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGSYANANSPVQIELQHTHDKAT
                     FEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHLWVTQNIWGDHTDPYGYLSEM
                     GVSKEKLA"
BASE COUNT          170 a           94 c          109 g          133 t
ORIGIN      
        1 ccaattaacg gatttgccta tactatcaac aatgaattta atttgggcgc aaatgaaggt
       61 agctcacaag tagcaaataa tcagtatatt ttactgcatg aaacggctaa tgaaacagca
      121 acaggacgca atgaagcgca gtatatgcaa cgttcatgga ctagcgctta tactgcttat
      181 attgtgggag acggcggaat tgtttatcaa gtcggtcaac ctggttatgt acagtacggt
      241 gctggttcgt atgctaatgc caacagtcct gtgcagattg agttacaaca cacacatgat
      301 aaagcaacgt ttgagaaaaa ctacaaggca tacgttgaat tggctagaga ttcagcaatg
      361 aaatatggta ttccattaac gttggacact ccttataacc aaccgggaat caaatcgcat
      421 ttatgggtaa cacaaaacat ctggggcgat catacagatc cttacggtta tctttctgaa
      481 atgggcgtaa gtaaagaaaa attagc
//
LOCUS       DACEUZ010000262          491 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.281, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000262 DACEUZ010000000
VERSION     DACEUZ010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..491
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.281"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(21..251)
                     /locus_tag="IXG44_002656"
     CDS             complement(21..251)
                     /locus_tag="IXG44_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1659340.1"
                     /translation="MMEEFIRKNISDEYADFYDQSSKKDKFQIDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
BASE COUNT          165 a           67 c           64 g          195 t
ORIGIN      
        1 ctcattaaaa agcataacca ttactccgat ctgtttttaa aattagtttc tacctctaat
       61 atatatcgag tttttatttt gccttcagag aatactgttt ctttttttgc agttacaggt
      121 tgtttatttt cggaaaaagc taatatagct aagattgaaa catcaatctg gaatttatct
      181 tttttactgc tttgatcata aaagtctgca tattcatcac tgatattttt tctaataaat
      241 tcttccatca taaaaatcac ctcaaaacga ttatacgaac ttacgttctt gttgtaaagc
      301 gatatttgag gggcaaaaaa ggggcataat ttgactgata ttaagttagc tattgatata
      361 tgaatgacat acaaaaatat aaaatctttt aaaaatgctt atatatcaat gtttttgcca
      421 ttcttttata tatctgttat atgtcaaaaa aatgtgttca atttcaattt gtatttgaat
      481 aaagaggttg t
//
LOCUS       DACEUZ010000263          487 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.282, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000263 DACEUZ010000000
VERSION     DACEUZ010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..487
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.282"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(<1..>487)
                     /locus_tag="IXG44_002657"
     CDS             complement(<1..>487)
                     /locus_tag="IXG44_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HAQ1659341.1"
                     /translation="IHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLDPFMD
                     MFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYRNKLK
                     EYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYYNNER
                     IK"
BASE COUNT          142 a           92 c           86 g          167 t
ORIGIN      
        1 gttttatccg ttcattattg tagtaataga tatagctatc gatggctgat tttaattcct
       61 cgaaacagtt ataaactttc ccgtgaaaaa tctcttgttt taataagcca aaaaaattct
      121 ccataagtga attatctaag cagttccctt tccgagacat gctttgaaag attttgtact
      181 ccttcaattt gtttctataa gctttcattt gatacgccca accttgatct gagtgaaacg
      241 tacgacgata gggacaatcg ttagtggtct ggatagcttc ctccaaacct tccattaccg
      301 ctaatgcatt gggcttttca gagatacgat aggataaaat ctcactattg aacatatcca
      361 taaaaggatc tagatacaat ttcttttgac ggataatgcc tttggcatcc acttcatggt
      421 atttaaattc agttgtatcc gtcgtgattt tctgatggca gatactggtg tagaaacgac
      481 gatggat
//
LOCUS       DACEUZ010000264          470 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.283, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000264 DACEUZ010000000
VERSION     DACEUZ010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..470
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.283"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            <1..>470
                     /locus_tag="IXG44_002658"
     CDS             <1..>470
                     /locus_tag="IXG44_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element IS1485 family transposase"
                     /protein_id="HAQ1659342.1"
                     /translation="ALSKRGIQISRRKISRIMKNRGLKSSYTVAYFKVHHSTCNEAKT
                     TNVLNRKFLRDNPLEAIVTDLTYVRVGKKWNYVCFILDLFNREILGYSCGEHKDAVLV
                     KKAFSRIKQPLTEVEIFHTDRGKEFDNQAIDELLTTFDINRSLSHKGCPFDNAV"
BASE COUNT          167 a           78 c           88 g          137 t
ORIGIN      
        1 aagccttatc aaaacgaggc attcagatca gccgacgaaa aattagtaga atcatgaaaa
       61 atagaggatt aaaatcgagc tatactgttg cttattttaa agtacatcat tctacttgca
      121 atgaagccaa aacgacaaac gtattgaatc gtaaattctt aagagacaac ccattagaag
      181 cgatcgtaac agacttgact tatgtacgag tcgggaaaaa atggaattat gtctgtttca
      241 ttttggatct gttcaatcga gaaattctcg gctattcttg tggagaacat aaagatgccg
      301 ttctagtaaa aaaagcattt agccgtatca aacaacctct gacagaggtt gagatttttc
      361 atactgatcg tggaaaagag tttgataacc aagctattga tgaattatta acaacttttg
      421 acatcaatcg atcattgagt cataaaggct gtccttttga taatgccgta
//
LOCUS       DACEUZ010000265          442 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.284, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000265 DACEUZ010000000
VERSION     DACEUZ010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..442
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.284"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            46..>442
                     /locus_tag="IXG44_002659"
     CDS             46..>442
                     /locus_tag="IXG44_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAQ1659343.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEI"
BASE COUNT          138 a           83 c           86 g          135 t
ORIGIN      
        1 atcctttaga attaatttgg ctaccaattc ataggaggat tcaagatgtc tcatactcag
       61 cttatcaaag ataccctaaa tattctagac ctaaatatct atttcgagga aaattgttta
      121 actaaagaaa aatacaaagg gcaaatttgc ttgatttaca aaggaactct tctgtacaag
      181 ccagaagagt gttcccattg tctttgtgtc gtcccctcta ggatcattcg ttgggggacg
      241 acaaccgttc ggttgttact gaatgatgtg tctgagtatc gcacttatct ggagctgaag
      301 aaacaacgat ttaaatgtaa gtcttgccaa agaacattcg tagcggatac ctctgttgct
      361 gaaaaacact gtttcattag tcaaaaagtt cgttggtctg tcattgctcg cttaaaagag
      421 aatacttcca tgacggaaat cg
//
LOCUS       DACEUZ010000266          441 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.285, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000266 DACEUZ010000000
VERSION     DACEUZ010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..441
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.285"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(5..90)
                     /locus_tag="IXG44_002660"
     tRNA            complement(5..90)
                     /locus_tag="IXG44_002660"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(54..56),aa:Leu,seq:taa)
     gene            complement(107..178)
                     /locus_tag="IXG44_002661"
     tRNA            complement(107..178)
                     /locus_tag="IXG44_002661"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(144..146),aa:Gly,seq:gcc)
     gene            complement(190..262)
                     /locus_tag="IXG44_002662"
     tRNA            complement(190..262)
                     /locus_tag="IXG44_002662"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(227..229),aa:Thr,seq:tgt)
     gene            complement(280..361)
                     /locus_tag="IXG44_002663"
     tRNA            complement(280..361)
                     /locus_tag="IXG44_002663"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(325..327),aa:Leu,seq:tag)
     gene            complement(369..441)
                     /locus_tag="IXG44_002664"
     tRNA            complement(369..441)
                     /locus_tag="IXG44_002664"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(406..408),aa:Lys,seq:ttt)
BASE COUNT          113 a          129 c          107 g           92 t
ORIGIN      
        1 gaaatgccga ggaccggaat cgaaccggta cggtgatcac tcaccgcagg attttaagtc
       61 ctgtgcgtct gccagttccg ccaccccggc gcgattgctt tggcaaagcg gaaaacgggg
      121 ttcgaacccg cgacccccac cttggcaagg tggtgctcta ccactgagct atttccgcgt
      181 gtttaaagat gccggctaaa ggacttgaac cctcgaccct ctgattacaa atcagatgct
      241 ctaccaactg agctaagccg gcatgtaaaa cttaaattat gcgggtgaag ggacttgaac
      301 ccccacgccg taaggcgcta gatcctaaat ctagtgcgtc tgccaattcc gccacacccg
      361 caaaaatatg agccgtacag ggctcgaacc tgtgaccctc tgattaaaag tcagatgctc
      421 taccaactga gctaacggct c
//
LOCUS       DACEUZ010000267          440 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.286, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000267 DACEUZ010000000
VERSION     DACEUZ010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..440
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.286"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            11..>440
                     /locus_tag="IXG44_002665"
     CDS             11..>440
                     /locus_tag="IXG44_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAQ1659344.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRA"
BASE COUNT          168 a           57 c           88 g          127 t
ORIGIN      
        1 ggtgcttttt ttgcggaaat atacatttgt ttttaagaaa aaagtagttt cagactattt
       61 aaataacgaa ggcggctaca aatatcttgc acataaatat caaataaatc gtacactggt
      121 tagacattgg gtaaggattt ataactatca tggttgggaa ggcttggttg gaggtggcaa
      181 aagctacact acaaaattta aacttgatgt tatagaatat atggaaacaa atggtctttc
      241 tatccaagaa actgctaaaa aatttaatat cggttcaaat agaactctaa gtaaatggat
      301 agagcaatat gaagaaggcg gtgcttcttc acttgagagc caaaaaaggg gcagaaaaat
      361 tagtatgaat tccaagctaa acattcctaa aaaacttaaa gatgagtctc ttgaagaaga
      421 agttattcgt ttaagagcag
//
LOCUS       DACEUZ010000268          428 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.287, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000268 DACEUZ010000000
VERSION     DACEUZ010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..428
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.287"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            82..>428
                     /locus_tag="IXG44_002666"
     CDS             82..>428
                     /locus_tag="IXG44_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HAQ1659345.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHV"
BASE COUNT          150 a           78 c           84 g          116 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgt
//
LOCUS       DACEUZ010000269          420 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.288, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000269 DACEUZ010000000
VERSION     DACEUZ010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..420
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.288"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(<1..>420)
                     /locus_tag="IXG44_002667"
     CDS             complement(<1..>420)
                     /locus_tag="IXG44_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ1659346.1"
                     /translation="CQVSLTTVIRTLKEFKSYLPKQSKKILPRVLMVDEFRSHASIED
                     KMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEEVEFLVTDMNAAYFQLTKRVL
                     PNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQK"
BASE COUNT          117 a           88 c           83 g          132 t
ORIGIN      
        1 cttttctgtc cggctttacg aagttcattc atttcacgga tacgcaactc attgaacgct
       61 tgattcatgt gtttgacaat atgaaaccga tcaatcacga ctttcgcatt tggcagaaca
      121 cgtttggtga gctggaagta ggcggcgttc atgtctgtca ccaagaattc tacttcttct
      181 ggattggtac aacctaagaa atagcttgtt aatcgaggta atttacgcgt aggcaaaaca
      241 tctattaatt ttcctgtttc gccatccgcg caaataaagc tcatcttatc ttctatggaa
      301 gcatgcgaac gaaattcgtc aaccatcaat actctgggga ggatcttctt agattgcttt
      361 ggtaaatagc ttttaaactc tttcaatgta cgaataacgg tggtcaaaga tacctgacag
//
LOCUS       DACEUZ010000270          408 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.289, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000270 DACEUZ010000000
VERSION     DACEUZ010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..408
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.289"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            66..290
                     /locus_tag="IXG44_002668"
     CDS             66..290
                     /locus_tag="IXG44_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HAQ1659347.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
BASE COUNT          196 a           48 c           81 g           83 t
ORIGIN      
        1 gtctaatcaa aaaaatataa aaattatttt gcgtacatat gattaaagaa aggagagtgt
       61 gacacatgga aacagaatgg caaagacgtg aaagaaaaaa acagaaaaaa gaagagatgt
      121 tacgattatg tcgattatta gatgtaaaag actgggacaa aaacgagcaa attgcacaaa
      181 aagtacgatc aatcatccat aaagaggaaa aagttaaaaa taaagacacg cgtagaactg
      241 aaaatgaaaa gagaaaagta caaaagaaaa gagaaaaatc aaaaagctag aaagaaaaag
      301 aggctgagac agaagcgttt aactccaaga aataagctgg aattcacaaa aatttgaaaa
      361 gcaattttcg tgaatttcag cttatttccg aaggagttgc ttctgctt
//
LOCUS       DACEUZ010000271          399 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.290, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000271 DACEUZ010000000
VERSION     DACEUZ010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..399
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.290"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            <1..382
                     /locus_tag="IXG44_002669"
     CDS             <1..382
                     /locus_tag="IXG44_002669"
                     /inference="COORDINATES: protein motif:HMM:NF024729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HAQ1659348.1"
                     /translation="KRMTVDCVIQTLNKAKIHYHIPEGMILHTDLGSQYTAREVEQWL
                     KTNKIRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDFEEANRALFSYIEGFYNR
                     NRIHSSIHYLTPQEFEELAKEKMA"
BASE COUNT          159 a           75 c           69 g           96 t
ORIGIN      
        1 aaaacgaatg actgtggatt gtgtcattca aacattgaat aaagcaaaaa tacattatca
       61 cattccagaa ggaatgattc tacacactga cttgggcagc caatacacag caagagaagt
      121 ggaacaatgg cttaaaacca acaaaataag gcattcttat agtagaaaag gaacacctta
      181 tgataatgct ggaatcgaat ctttccatgc ctcattgaaa aaagaagaag tctacacgac
      241 tagctactca gattttgaag aagcaaatcg agcgctattt agctacattg aaggatttta
      301 taaccgaaat cgaatccata gctcgattca ctatctaacc ccacaggaat ttgaagaatt
      361 agcaaaagaa aaaatggctt aaccgtctct actaaaatg
//
LOCUS       DACEUZ010000272          395 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.291, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000272 DACEUZ010000000
VERSION     DACEUZ010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..395
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.291"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(35..319)
                     /locus_tag="IXG44_002670"
     CDS             complement(35..319)
                     /locus_tag="IXG44_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HAQ1659349.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
BASE COUNT          111 a           86 c           60 g          138 t
ORIGIN      
        1 agaggttgtg acacgtcttt tgtcacaacc tcttttattt ttgcttcact gtaactgcta
       61 caatatcacc gatttctttt ccgatgtttt tacgaatatc ttttcgtagt ccaaggatgt
      121 agcagactga accatctgga tttttcacgc ccatattcac gatactgcct tcatatggca
      181 cattgtcaaa agttgcttgg actttaactc ttccagcacc gaattctttt cttatatcat
      241 acggaaaaat aatatatgcc ccgcccttgc cgacttcgga agcatagatt tttgattcaa
      301 aagaataaat ttccctcatt ttatttctcc tattttttac atctagtata gcatagcaaa
      361 aaaaagaagg aactgtgaca aaagtcttgt cgcag
//
LOCUS       DACEUZ010000273          384 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.292, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000273 DACEUZ010000000
VERSION     DACEUZ010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..384
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.292"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            <1..357
                     /locus_tag="IXG44_002671"
     CDS             <1..357
                     /locus_tag="IXG44_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HAQ1659350.1"
                     /translation="DTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQ
                     GTEHRTIKYLNNLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKTRKEGTLF
                     GFSVCTEIKVLLGIPA"
BASE COUNT          131 a           76 c           76 g          101 t
ORIGIN      
        1 gacacacaag cagcctatgc ttttcttaag cggttagtga agcagtttga tgaaccgaag
       61 gttgtagtca cagataaagc cccctctatt acaagtgcct ttaagaaact aaaagaatac
      121 ggcttttatc aagggacaga acatcgtacc attaaatacc tgaataattt gattgaacaa
      181 gaccatcgtc cagtaaagag acgcaataaa ttctatcgaa gtttacgcac tgcctctacc
      241 acgattaaag gcatggaagc cattcgagga ttatataaga aaacccgaaa agaaggcact
      301 ctcttcgggt tttcggtctg tactgaaatc aaggtattat tgggaatccc agcttaaatc
      361 atagataccg taagggattt tatt
//
LOCUS       DACEUZ010000274          380 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.293, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000274 DACEUZ010000000
VERSION     DACEUZ010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..380
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.293"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(<1..>380)
                     /locus_tag="IXG44_002672"
     CDS             complement(<1..>380)
                     /locus_tag="IXG44_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ1659351.1"
                     /translation="FLIAAQQLEKMGYVNENYLNGLIAREREFPTGLITKYLNIALPH
                     SECEYVEKPFVFVMQLKNPIYFYQMGDNQEMTVENLFFLGIKEPKEQVGLLQAFMNLF
                     MDETFVENYKKQETPQNIYELITN"
BASE COUNT          113 a           69 c           53 g          145 t
ORIGIN      
        1 atttgtaata agttcgtata tgttttgcgg tgtttcttgt ttcttgtaat tttccacgaa
       61 tgtttcatcc atgaataaat tcataaacgc ttgaagtaat cctacttgtt cttttggttc
      121 tttgattcct aaaaaaaata gattttctac agtcatttcc tgattatccc ccatttgata
      181 aaaataaatt gggttcttca gctgcataac aaaaacaaat ggcttttcga catattcaca
      241 ttccgaatgt ggcagcgcga tatttaaata cttagtaatc aatccagtag gaaattctct
      301 ttctcttgca atcagtccat tcaaataatt ctcattgacg taccccattt tttctaattg
      361 ttgtgcagcg attaaaaaag
//
LOCUS       DACEUZ010000275          376 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.294, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000275 DACEUZ010000000
VERSION     DACEUZ010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..376
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.294"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            82..>376
                     /locus_tag="IXG44_002673"
     CDS             82..>376
                     /locus_tag="IXG44_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HAQ1659352.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTH"
BASE COUNT          133 a           62 c           76 g          105 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcacgac aaaataaaca
       61 actgagagga cgatatgcta tatgaatgat tctatcaaaa aaatgctgcg attaatagag
      121 aaagatttga tgattacaga ggtctcttac gagacctttc agaagaaaaa gacgttgatc
      181 gtcgatgctg ttttctcacc tgctccccat acctgtagaa attgtggttc tactgtggta
      241 gatggaaacg ggaaagtaat tgtagtgaaa aatgggaaga aggaaacgat tgtccgcttt
      301 gaacaataca atcacatgcc tttggttatg cgcttaaaaa agcagcgcta tacctgtaaa
      361 aactgtcgaa cccatt
//
LOCUS       DACEUZ010000276          375 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.295, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000276 DACEUZ010000000
VERSION     DACEUZ010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.295"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            <1..>375
                     /locus_tag="IXG44_002674"
     CDS             <1..>375
                     /locus_tag="IXG44_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ1659353.1"
                     /translation="MTKSFTEEVTSFKERELHDRYAAIYMDATYIPLKRKTVAKEAIH
                     IAVGIRPDGSKKVLSYAIAPTESITIWEEILIDLQERGVKNVLLFITDGLKGMAGAVQ
                     RFYPKARFQHCCVHVSRNIVHKV"
BASE COUNT          104 a           85 c           87 g           99 t
ORIGIN      
        1 atgactaaat catttacgga agaagtcact tcatttaagg aacgtgagct tcatgaccgt
       61 tatgccgcta tttatatgga cgcaacctat atccctttaa agcggaaaac cgtcgccaag
      121 gaagccattc atatcgcagt tggcattcgc ccggacggtt caaagaaagt gttgagctat
      181 gcgattgcac caactgaatc tatcacgatt tgggaagaga ttttaataga cctccaggaa
      241 cgtggcgtga aaaacgtcct ccttttcatc acggatggct taaaggggat ggcaggggcc
      301 gtgcagcggt tctaccccaa agcccgtttt caacattgct gtgtgcacgt ttcccgtaat
      361 attgttcata aagta
//
LOCUS       DACEUZ010000277          360 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.296, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000277 DACEUZ010000000
VERSION     DACEUZ010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..360
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.296"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
BASE COUNT          116 a           73 c           73 g           98 t
ORIGIN      
        1 tagaacacaa acaaacatct tagaaagcgt ctgggcgcaa atctaaggat aaaaacgacg
       61 actgaatcag gacgttctta ccagagcgag tcgaagtgtt tatctatacg ataataataa
      121 cttgaagagg tagtgaaaga gacgtttagc cttattgccg ataggctgtc tcttgaacac
      181 tgtttattca gaataaggga ctgtgacaaa aaccgtgtca cagtcccttt ttccgaacaa
      241 acggtgttca aaagcttgca tctacggcac taagcccaat cagatgaaaa atatggagtg
      301 ctatttttat ctgattgttc ttactgcttg atgcagagca gctttttcac aacctctcca
//
LOCUS       DACEUZ010000278          344 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.297, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000278 DACEUZ010000000
VERSION     DACEUZ010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.297"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            42..248
                     /locus_tag="IXG44_002675"
     CDS             42..248
                     /locus_tag="IXG44_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1659354.1"
                     /translation="MVKFPQFKLNPAASIQFQGHVFDVLAKDNIKKYKKGNIFHIDIQ
                     ISSNLEDIKKAFKIKTIGTAPNFK"
BASE COUNT          155 a           45 c           41 g          103 t
ORIGIN      
        1 gtaaaaaaat aaaaaaatgt gacaagtgtt ttgaatcagc tgtggtaaag tttcctcaat
       61 ttaaactaaa tccagctgcc tcgatacaat ttcaaggaca tgtatttgat gttttagcta
      121 aagataacat aaaaaaatac aaaaaaggta atatattcca tattgatata caaatatctt
      181 ctaatttaga agatataaaa aaagccttta aaattaaaac aattggcact gctcctaatt
      241 ttaaataaca aaaaaattac tagataatct aaaaacagga tggcattatg agaacaataa
      301 ctaaacttcc tatatcacat attttgtaat tagtgaaaag caaa
//
LOCUS       DACEUZ010000279          337 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.298, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000279 DACEUZ010000000
VERSION     DACEUZ010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..337
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.298"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            122..328
                     /locus_tag="IXG44_002676"
     CDS             122..328
                     /locus_tag="IXG44_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAQ1659355.1"
                     /translation="MNNRTFDILKWIAIVVIPALATFVGLVGKGVNWRYTDITVMIIT
                     GFGAFLGSILGVSNRTYKKISSDK"
BASE COUNT          120 a           43 c           82 g           92 t
ORIGIN      
        1 gataaaattg aacaactgga cgggcaaatt gatgccttgc tttcagaaag agaaatattg
       61 atagaagaaa acaagcgatt gaaaaagaaa gtagcccaac caatgaaggg aggtggatag
      121 tatgaacaat agaacctttg atattttgaa atggatcgct atagtagtta tccccgcttt
      181 agcgactttt gtcggtcttg tcggaaaagg tgttaactgg cggtacacag atataacggt
      241 gatgattatt acaggttttg gagcatttct agggagtatt ttaggtgtat caaatcgaac
      301 gtataagaaa atttcatcag ataaatagga ggataga
//
LOCUS       DACEUZ010000280          332 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.299, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000280 DACEUZ010000000
VERSION     DACEUZ010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..332
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.299"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            89..>332
                     /locus_tag="IXG44_002677"
     CDS             89..>332
                     /locus_tag="IXG44_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ1659356.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNSISDINLIYSGQQLTIPTEGS"
BASE COUNT          107 a           70 c           51 g          104 t
ORIGIN      
        1 cttttaccta tgaaatattt tcacgtgtta aagtattcct cgttgaacac aacaaataaa
       61 actttttaac gaataaagga gatttttcat gacatcactt aaaactttac tttttggaac
      121 aactttggct gccggcgctg cattcttcat gggaacgact gctcatgcgg acgaagctta
      181 taccgttcaa tcaggtgata ctctatcaac gatttctcaa aaatacgttg gtgataactc
      241 cttgatcaat gccattgcag aatcaaattc aatttctgat attaacctga tttactcagg
      301 acaacaatta acaatcccaa cagaaggctc tg
//
LOCUS       DACEUZ010000281          323 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.300, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000281 DACEUZ010000000
VERSION     DACEUZ010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..323
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.300"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
BASE COUNT          109 a           44 c           41 g          129 t
ORIGIN      
        1 aattataaaa aaattttgca gtttttctta ctttttttgt cgtttaattg aatgataaaa
       61 accagtttct ttattcagtt aatcaaatat gaacgaatta gatatgaatc attgtatttg
      121 ccgttgcgga aggatttttt aattaaccac agataaacat tgataacgat tcgcttattg
      181 taatcaacag attaaatttc atatgttcat atgtacggat acattttcgg accgattaat
      241 tttaaccatt ctatttattt gcttacctat tagatatcaa taaaaaactg cttaacagct
      301 atttcagctg ttaagcagtt tta
//
LOCUS       DACEUZ010000282          301 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.301, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000282 DACEUZ010000000
VERSION     DACEUZ010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..301
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.301"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            <1..>301
                     /locus_tag="IXG44_002678"
     rRNA            <1..>301
                     /locus_tag="IXG44_002678"
                     /product="23S ribosomal RNA"
BASE COUNT           93 a           59 c           87 g           62 t
ORIGIN      
        1 ataagggcgc acggtggatg ccttggcact aggagccgat gaaggacggg actaacaccg
       61 atatgctttg gggagctgta cgtaagctat gatccagaga tttccgaatg ggggaaccca
      121 acatctttaa taggatgtta cgattgtgtg aatacatagc acattcgagg tagacgcaga
      181 gaactgaaac atctaagtac ctgcaggaag agaaagaaaa ttcgattccc tgagtagcgg
      241 cgagcgaaac gggaaaagcc caaaccagca agcttgcttg ttggggttgt aggactccaa
      301 t
//
LOCUS       DACEUZ010000283          299 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.302, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000283 DACEUZ010000000
VERSION     DACEUZ010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..299
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.302"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            <1..>299
                     /locus_tag="IXG44_002679"
     CDS             <1..>299
                     /locus_tag="IXG44_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ1659357.1"
                     /translation="TIVGIHHKSAVITLVERLSKAIIALKPEGRKAVDIENSINEWLQ
                     SVPKNLFKSITFDCGKEFSNWKSISNTNDIDIYFADPGTPSQRGLNEHSNGLLRK"
BASE COUNT          102 a           63 c           54 g           80 t
ORIGIN      
        1 acgattgtcg gcatccacca taaaagtgcc gtcatcacac tcgttgagcg actttcaaaa
       61 gccattatcg ccttgaaacc agaaggccgt aaggcagttg atattgaaaa ttcgattaat
      121 gaatggcttc aatccgtacc caaaaatctt ttcaaatcaa ttacctttga ttgtggaaaa
      181 gaattctcta attggaaaag tattagtaat acgaatgata ttgatattta tttcgcagac
      241 ccaggaacgc cttcccaacg gggattaaat gaacattcaa acggactcct ccgaaaaga
//
LOCUS       DACEUZ010000284          296 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.303, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000284 DACEUZ010000000
VERSION     DACEUZ010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..296
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.303"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(<1..>296)
                     /locus_tag="IXG44_002680"
     CDS             complement(<1..>296)
                     /locus_tag="IXG44_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ1659358.1"
                     /translation="KIPHFSTFGKNYVRRFRETTVFEDIFAYILEQAVKAGFVTEDNL
                     YLDSTHIKANANKHKFTKEMTHDEAKAYQDELEDEINRQRIEAGKRPFTLDLEK"
BASE COUNT           80 a           60 c           50 g          106 t
ORIGIN      
        1 tccttttcta agtccaaagt aaaagggcgt tttccagcct ctattcgttg acgattgatc
       61 tcatcttcta attcatcctg ataggctttc gcttcatcat gggtcatttc tttcgtaaac
      121 ttatgtttat tggcgttagc tttgatgtgg gtagaatcca gatacaaatt atcctctgtt
      181 acaaaacctg ctttaactgc ttgttctaaa atataggcaa aaatatcctc aaacaccgtc
      241 gtttctctaa atcggcgaac ataattcttt ccaaaagttg agaaatgggg gatttt
//
LOCUS       DACEUZ010000285          292 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.304, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000285 DACEUZ010000000
VERSION     DACEUZ010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..292
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.304"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            <1..185
                     /locus_tag="IXG44_002681"
     CDS             <1..185
                     /locus_tag="IXG44_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAQ1659359.1"
                     /translation="YTFETLQQLDLELFDYVNWWNHLRLHGTLGYETPVGYRNQRLAQ
                     RILDNELGCANASEAV"
BASE COUNT           83 a           63 c           69 g           77 t
ORIGIN      
        1 aatacacatt tgaaacctta caacaattgg atttggagtt atttgactat gtcaattggt
       61 ggaaccacct tcggttgcac ggtacacttg gctacgagac accggttggt taccgtaacc
      121 agagattggc gcagcgaatc cttgataatg agctcggatg tgctaacgct agcgaggcag
      181 tctaacttta actgttagct cctgccgaag atcgtcacat ccgaggaggc tcattgtcaa
      241 ggacaatcgg aatagcatac ggaaagaagt tctgcacctt ataaaatttg tc
//
LOCUS       DACEUZ010000286          291 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.305, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000286 DACEUZ010000000
VERSION     DACEUZ010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..291
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.305"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            76..>291
                     /locus_tag="IXG44_002682"
     CDS             76..>291
                     /locus_tag="IXG44_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ1659360.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTALEYFEQYKENKRRCGRTEIIF"
BASE COUNT          111 a           55 c           50 g           75 t
ORIGIN      
        1 agacaaataa aaaagccatt tatgttacac tcaaattgta atacccgcta aagcatacaa
       61 ggagagtgaa cataaatgac ttatacccat cttacaccaa acgagcttgt aatgatagaa
      121 gcatattttc atcaagaaac tccggttgct atcgttgcga agcagcttaa acgtggacgc
      181 caaacaattt acaatgtcta taactttctc aaatgtggtg gaacagcact tgaatacttt
      241 gaacaataca aagaaaataa gcgacgttgt gggagaaccg aaatcatttt t
//
LOCUS       DACEUZ010000287          291 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.306, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000287 DACEUZ010000000
VERSION     DACEUZ010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..291
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.306"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            <1..>291
                     /locus_tag="IXG44_002683"
     CDS             <1..>291
                     /locus_tag="IXG44_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ1659361.1"
                     /translation="ATISRHSSGKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILS
                     DGQKIDNNKFTSKMEKKLKREQRKLSRRALLAKNKGTNLFEAKNYQKQKRKVA"
BASE COUNT          115 a           44 c           46 g           86 t
ORIGIN      
        1 gctacaatat cacgtcattc tagcggtaaa tactatattt ctttgttgtg taaagaagaa
       61 attatcgaat tacctaaaac taattccaca attggtattg acttaggtat tatggacttt
      121 gcgattcttt ctgatggaca aaaaattgat aataataaat tcacgtccaa aatggaaaag
      181 aaactaaagc gtgaacaacg caaattgtct aggcgtgctt tattggctaa aaataaaggt
      241 accaatctat ttgaagctaa aaattatcaa aaacaaaaac gcaaagtagc t
//
LOCUS       DACEUZ010000288          291 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.307, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000288 DACEUZ010000000
VERSION     DACEUZ010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..291
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.307"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            <1..>291
                     /locus_tag="IXG44_002684"
     CDS             <1..>291
                     /locus_tag="IXG44_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ1659362.1"
                     /translation="ATISRHSSGKYYISLLCKEEVRELPKSNSAVGIDLGIIDFAILS
                     TGQKFDNNKFTSKMEKKLKREQRKLSRRALLAKQKGINLFEARNYQTQKRKVA"
BASE COUNT          110 a           48 c           46 g           87 t
ORIGIN      
        1 gctacaatat cacgtcattc tagcggtaaa tattatattt ctttgttatg caaagaagaa
       61 gttcgcgaac tacctaaatc taattctgct gttggtattg acctaggtat tatagacttt
      121 gcaattcttt ctaccggtca aaaatttgat aataataaat ttacgtccaa aatggaaaag
      181 aaactcaaac gtgaacaacg taagttgtca agacgtgcct tattagctaa acaaaaaggt
      241 atcaatctgt ttgaagctag aaattatcaa acacaaaaac gtaaagtagc a
//
LOCUS       DACEUZ010000289          276 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.308, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000289 DACEUZ010000000
VERSION     DACEUZ010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..276
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.308"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
BASE COUNT          120 a           49 c           30 g           77 t
ORIGIN      
        1 aagggacttc cttttttcta tttacacaaa atattctatt ttgttatcac tgttgaatca
       61 tcatttataa gtgaacgtaa aagataatag atacccaaaa acagaaagaa aaataacggt
      121 aatacacgga gtaaaacaaa cggagtttca aaaatgaacg ctttccaaaa ccacataccc
      181 tccatttccc ataaaccttg ggtaaaatac catgatttaa agttcaatat cttatccaaa
      241 ttccaagaat tactactaaa aaaaaataaa gaaata
//
LOCUS       DACEUZ010000290          275 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.309, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000290 DACEUZ010000000
VERSION     DACEUZ010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..275
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.309"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(58..142)
                     /locus_tag="IXG44_002685"
     tRNA            complement(58..142)
                     /locus_tag="IXG44_002685"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(106..108),aa:Leu,seq:caa)
     gene            complement(163..233)
                     /locus_tag="IXG44_002686"
     tRNA            complement(163..233)
                     /locus_tag="IXG44_002686"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(199..201),aa:Cys,seq:gca)
BASE COUNT           84 a           61 c           57 g           73 t
ORIGIN      
        1 ataaaagatg ttatttaatt tctcaataaa aaactaagat gaagacatct tagtcaatac
       61 cggcggccgg ggtcgaaccg gcacgccctc aacgggcact ggattttgag tccagcgcgt
      121 ctgccaattc cgccacgccg gcaagttgat ttattagtac taaggcggta accggatttg
      181 aaccggtgat gaaggttttg cagacctctg ccttaccact tggctatacc gccattatac
      241 tattatcaat ctataaaata aagaataaat tatag
//
LOCUS       DACEUZ010000291          273 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.310, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000291 DACEUZ010000000
VERSION     DACEUZ010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..273
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.310"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            <1..>273
                     /locus_tag="IXG44_002687"
     CDS             <1..>273
                     /locus_tag="IXG44_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ1659363.1"
                     /translation="RNLDSRTRFEQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYY
                     WVKKLDKPDKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINH"
BASE COUNT          121 a           37 c           43 g           72 t
ORIGIN      
        1 agaaaccttg attcaagaac aagatttgag caagaagaaa tcaaggttaa aacaatcctt
       61 cagctcaaaa aagaatttaa attgaatcta ttactttcaa ttgcacaatt agcgaagtca
      121 acttattatt actgggtaaa aaaattagat aagccagata aatatagcaa gataaagcaa
      181 gaaattacag cgattgtaaa agaatcaaga aactcttatg gttatcgtag agtcacttta
      241 gcgttaaaga tgaaaggata tacaatcaat cat
//
LOCUS       DACEUZ010000292          273 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.311, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000292 DACEUZ010000000
VERSION     DACEUZ010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..273
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.311"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            <1..>273
                     /locus_tag="IXG44_002688"
     CDS             <1..>273
                     /locus_tag="IXG44_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1659364.1"
                     /translation="SDADKYNPEGQKVTTELNKEPDAFEGIKNKEDLPKDTKYTWKEK
                     VDVSAAGNKKGTVVVTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEG"
BASE COUNT          111 a           38 c           64 g           60 t
ORIGIN      
        1 atctgacgct gacaaatata atcctgaagg tcaaaaagtg actacagaat tgaataagga
       61 accagacgca tttgagggaa ttaagaacaa agaagattta ccaaaagata ctaagtatac
      121 ttggaaagaa aaagtagatg ttagcgcagc tggaaataaa aaagggactg ttgtagtgac
      181 atatccagat ggatcatctg atgaagttga agtagatgtt acagtgacag acaatcgttc
      241 tgacgccgat aaatatgagc caacagtaga agg
//
LOCUS       DACEUZ010000293          273 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.312, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000293 DACEUZ010000000
VERSION     DACEUZ010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..273
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.312"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
BASE COUNT          106 a           47 c           44 g           76 t
ORIGIN      
        1 tttccttcca tcagcataaa agttcacctc aaaaagagta tacgaacgaa tgttcttttt
       61 gtgaaggcgg aatttaatat actgaataat agaaaacaag tacttgtgcc aaattatgtg
      121 ccaaaaaaat tcgaattcaa caaaatgtaa acaaatagaa aaccttctat attaagattt
      181 cctgcaataa caaacacaac agaagacgtg taatagttag tcaggaacgt acaaataacc
      241 cctgtatcct ttgcggtaca gggggttatt ttg
//
LOCUS       DACEUZ010000294          263 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.313, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000294 DACEUZ010000000
VERSION     DACEUZ010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..263
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.313"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            <1..>263
                     /locus_tag="IXG44_002689"
     CDS             <1..>263
                     /locus_tag="IXG44_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ1659365.1"
                     /translation="LTDGTNHAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTG
                     KELARVRADGIYRPDVNQAYNTSGNVGYHVSFNMRNFPSKKVYV"
BASE COUNT           89 a           42 c           54 g           78 t
ORIGIN      
        1 ctgacagatg gaacaaatca cgcccacatt gatcagtttg gggaaatcga aaacgcaaac
       61 ttgcatgtgg ctggatggca cattgctaac tataaatacg agtatatttt cattatggac
      121 tacaatactg ggaaagaatt agctcgagta agagctgatg gaatttatag accagatgta
      181 aaccaagctt ataatacttc tggaaatgtt ggttatcatg tatctttcaa tatgcgtaat
      241 tttcctagta agaaagtcta tgt
//
LOCUS       DACEUZ010000295          263 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.314, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000295 DACEUZ010000000
VERSION     DACEUZ010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..263
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.314"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            <1..>263
                     /locus_tag="IXG44_002690"
     CDS             <1..>263
                     /locus_tag="IXG44_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ1659366.1"
                     /translation="LTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTG
                     KELARVRADGIYRSDVNQAYNTSGNVGYHVSFNMRNFPNKKVYV"
BASE COUNT           91 a           40 c           52 g           80 t
ORIGIN      
        1 ctgacagatg gaacgaatta cgcccacatt gatcagtttg gagaaatcga aaatgcaaat
       61 ttgcatgtag ctggatggca cattgctaac tataaatacg agtatatctt cattatggat
      121 tacaatactg ggaaagaatt agctcgagta agagctgatg gaatttatag atcagatgta
      181 aatcaagctt ataatacttc tggaaatgtt ggctatcatg tatcttttaa catgcgtaac
      241 ttccctaata agaaagtcta tgt
//
LOCUS       DACEUZ010000296          254 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.315, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000296 DACEUZ010000000
VERSION     DACEUZ010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.315"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
BASE COUNT           74 a           43 c           54 g           83 t
ORIGIN      
        1 ttgagctaat accgaaggtc agctgattga acaccgttta agaaagaggt cgtgagcctg
       61 tcatttaact ctgagtcaca agtaacaatt tcaaaaaaca gcttctcatc tttgaggaat
      121 atcgtgactt gtggacgagg cgttgataag tgaatcatct ttttgttacc tattttttct
      181 gtaaccaaaa aacgatggaa cgttgttttg attgccctta atgaggacag tcggaacagg
      241 tttgtcattt ttat
//
LOCUS       DACEUZ010000297          249 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.316, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000297 DACEUZ010000000
VERSION     DACEUZ010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..249
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.316"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            <1..>249
                     /locus_tag="IXG44_002691"
     CDS             <1..>249
                     /locus_tag="IXG44_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ1659367.1"
                     /translation="SKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFEFIYPIVEA
                     TYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDT"
BASE COUNT           71 a           50 c           47 g           81 t
ORIGIN      
        1 gtctaaaaac aatacgaatg gtagaaatca atttgcgatg cttacgattg atgatctagt
       61 tcctcaagat catctcgttc gaaagattga tgccgcgctt gattttgaat ttatttatcc
      121 aatcgtcgaa gccacttact ccgatttagg tcgcccaagt atcgatccag tgattctgat
      181 caaattggtg tttattcagt atttattcgg tatccgttcc atgcgacaaa ccattaagga
      241 agtcgatac
//
LOCUS       DACEUZ010000298          247 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.317, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000298 DACEUZ010000000
VERSION     DACEUZ010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..247
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.317"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
BASE COUNT           78 a           43 c           54 g           72 t
ORIGIN      
        1 tttatcgaga ggttgtgaaa tcagcgaatt aacctgagta ttagagaaaa aaatggaaaa
       61 atagtccctc atattttacc gtttgttgaa ctaataccga gggtcagctg attgaacacc
      121 gtttagtgga gaggtcgtaa gcctaccccc cagctcctag ttacaaggac aaattcaaaa
      181 aacagctctt catattttat gaatgttgta cttgtgattc ggaggtggca agggaacact
      241 gtttatt
//
LOCUS       DACEUZ010000299          246 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.318, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000299 DACEUZ010000000
VERSION     DACEUZ010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.318"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            <1..168
                     /locus_tag="IXG44_002692"
     CDS             <1..168
                     /locus_tag="IXG44_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HAQ1659368.1"
                     /translation="GFKVYANYYHARAKHSATAKNGRGGQVRSVQKAGVRAYATCNAT
                     LTGNTGWWNVY"
BASE COUNT           93 a           34 c           48 g           71 t
ORIGIN      
        1 ggatttaaag tttatgcaaa ttactaccat gctagagcta aacatagtgc aacagctaaa
       61 aatggacgag gcggtcaagt acgatcagtt cagaaagctg gagtaagagc ttatgcaaca
      121 tgcaatgcca cacttactgg aaatactggt tggtggaatg tttattaaaa aagagagttt
      181 aaactctctt ttttatttac atttgaaagg aaattcaaaa tatgttaaaa aaaataaacg
      241 ctgttt
//
LOCUS       DACEUZ010000300          244 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.319, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000300 DACEUZ010000000
VERSION     DACEUZ010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..244
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.319"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(<1..>244)
                     /locus_tag="IXG44_002693"
     rRNA            complement(<1..>244)
                     /locus_tag="IXG44_002693"
                     /product="23S ribosomal RNA"
BASE COUNT           52 a           81 c           49 g           62 t
ORIGIN      
        1 ttggacagac atttccgatc gtctgcattc cttagcctcc tgcgtccctc cattgctcaa
       61 acaaaaacaa cgggtacagg aatatcaacc tgttatccat cgcctacgcc tgtcggcctc
      121 ggcttaggtc ccgactaacc ctgggcggac gagccttccc caggaaacct tagtcattcg
      181 gtggacagga ttctcacctg tctttcgcta ctcataccgg cattctcact tctaagcgct
      241 ccag
//
LOCUS       DACEUZ010000301          237 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.320, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000301 DACEUZ010000000
VERSION     DACEUZ010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..237
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.320"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            <1..>237
                     /locus_tag="IXG44_002694"
     CDS             <1..>237
                     /locus_tag="IXG44_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ1659369.1"
                     /translation="TIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWFS
                     RFPKNFFKSITFDCGKEFSNWKAISNQHDIDIYFA"
BASE COUNT           77 a           44 c           37 g           79 t
ORIGIN      
        1 acgatcgttg gcattcatca taaaagtgcc gtcattactt tagttgaaag attatctaaa
       61 gtcattatca cgattaaacc caacggccgt aaggcattag atattgaaac tgcccttaat
      121 caatggtttt ctcgcttccc taaaaacttc tttaaatcta ttacgtttga ctgtggaaaa
      181 gaattttcta actggaaagc cattagtaac caacatgata ttgatatata ttttgcg
//
LOCUS       DACEUZ010000302          236 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.321, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000302 DACEUZ010000000
VERSION     DACEUZ010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..236
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.321"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            26..>236
                     /locus_tag="IXG44_002695"
     CDS             26..>236
                     /locus_tag="IXG44_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAQ1659370.1"
                     /translation="MSYTHLIKETLDILDLSVTFNENCLTKEKYKGQICHIYRGNLIY
                     TAQECIHCKHQIASDIVRWGTTTVRL"
BASE COUNT           86 a           48 c           39 g           63 t
ORIGIN      
        1 aaccaaccca aaaggaggaa tcaagatgtc ttacactcac cttatcaaag aaacattaga
       61 tattctagac ttatcagtta cttttaacga aaattgtctc acaaaagaaa aatacaaagg
      121 gcaaatctgt catatttatc gtggaaactt gatttataca gcccaagaat gcatccactg
      181 taaacaccag attgcttcag atattgtgcg ttggggtaca acaaccgtcc ggttgt
//
LOCUS       DACEUZ010000303          231 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.322, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000303 DACEUZ010000000
VERSION     DACEUZ010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..231
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.322"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
BASE COUNT           84 a           36 c           22 g           89 t
ORIGIN      
        1 tcttgagtat accctttttt cactcttaat tctcttagct tagatcctaa atcctccatt
       61 ttttctccta tgtaaacgat tatttacata aaattttatc ataaaaatac taacgcgcca
      121 aaaaagttct ttattttata aaacacacta aaataaaata attctagtaa aaaagatagg
      181 agaaataagt tatgaaaaaa tttttatgtc tttctgcttt aatgggtgtc t
//
LOCUS       DACEUZ010000304          229 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.323, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000304 DACEUZ010000000
VERSION     DACEUZ010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..229
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.323"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
BASE COUNT           79 a           35 c           45 g           70 t
ORIGIN      
        1 gagaaaaagc atggaatgcg ctgcatcagg tatcgaggct tagaaaaaaa tcaggtgcat
       61 acgatgcttg tttttgccga aatgagtctg aaaaaattgg tcacttagca gtgggagcat
      121 atgtcttcat attttttatt atacaaaaat agacaaaagt atcagtctac actcttagta
      181 aagaggaaac tgatactttt gtctacagta tgatatttct tacatctac
//
LOCUS       DACEUZ010000305          227 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.324, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000305 DACEUZ010000000
VERSION     DACEUZ010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..227
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.324"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            1..227
                     /locus_tag="IXG44_002696"
                     /pseudo
     CDS             1..227
                     /locus_tag="IXG44_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
BASE COUNT           94 a           43 c           44 g           46 t
ORIGIN      
        1 tacgccttct tctttcgata aatggatgaa gcaagcacaa tcaacgggct cattcaaaga
       61 aagagacaac ttaacaccag aacaagcaga attgatcgca ctaagaaaga aaaataagca
      121 actcgaaatg gagaatgata ttttaaagca agcggcgctg atattcggac gaaaagacaa
      181 gtaattgatg ccaacaagca taaatattcc atatcagcga tgtgcaa
//
LOCUS       DACEUZ010000306          224 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.325, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000306 DACEUZ010000000
VERSION     DACEUZ010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..224
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.325"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            <1..>224
                     /locus_tag="IXG44_002697"
     CDS             <1..>224
                     /locus_tag="IXG44_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HAQ1659371.1"
                     /translation="PSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAA
                     ESQTPAKPAAEPTVDTESAGSSSEGVFQFK"
BASE COUNT           70 a           47 c           58 g           49 t
ORIGIN      
        1 ccgtcacctg ttacaggaac ggttaaaaat gtgattgttc cagaaggtac cgtagcaaat
       61 gttggggatg tattagtaga aatcgatgca ccaggacacg aagacaatga aggggacagt
      121 ggtgtagccg cagaatccca aactccagct aaaccggctg ctgaacccac tgttgataca
      181 gaatcagctg gatcgtcaag cgaaggagtc ttccaattta agtt
//
LOCUS       DACEUZ010000307          223 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.326, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000307 DACEUZ010000000
VERSION     DACEUZ010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..223
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.326"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            <1..>223
                     /locus_tag="IXG44_002698"
     CDS             <1..>223
                     /locus_tag="IXG44_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ1659372.1"
                     /translation="NSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDT
                     LEETVIHLFRKGITMSEIADLIEKMYGHH"
BASE COUNT           63 a           56 c           53 g           51 t
ORIGIN      
        1 gtaattctcg taacggctcc tatgaccgta cggtcaagac cgagtacggg gaacttcatc
       61 tccagattcc gcgcgaccgc aacggcgagt tcaagcaaca gactgttcct gcttatagac
      121 ggacgaatga taccttagag gagaccgtca ttcacctctt ccgaaaaggt attaccatgt
      181 cggaaatcgc agacttgatt gagaaaatgt atgggcatca cta
//
LOCUS       DACEUZ010000308          217 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.327, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000308 DACEUZ010000000
VERSION     DACEUZ010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..217
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.327"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            <1..>217
                     /locus_tag="IXG44_002699"
     CDS             <1..>217
                     /locus_tag="IXG44_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ1659373.1"
                     /translation="LNQIAKVGYNSTKKSYFYGLKIHMIVTKTGFPITYSITNPGVHD
                     VKVLETLSEEANLPNILGDKGYISHKI"
BASE COUNT           77 a           40 c           36 g           64 t
ORIGIN      
        1 tattgaatca aatagccaaa gttggctata actcaacaaa aaaatcttat ttttatggtc
       61 ttaaaattca tatgatcgtt actaagactg gctttccgat tacttactca atcacgaatc
      121 caggtgtcca tgacgtgaaa gtgttagaga ctttatcgga agaagcgaat cttcccaata
      181 tcctagggga caaaggatat attagccata aaatcca
//
LOCUS       DACEUZ010000309          214 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.328, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000309 DACEUZ010000000
VERSION     DACEUZ010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..214
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.328"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
BASE COUNT           87 a           25 c           38 g           64 t
ORIGIN      
        1 aaagcttcga aatccattgg catttcgttg gaataccttg atatgattat tcaaatataa
       61 aatatagaga gaagagagag agggaagata tttataaagg gcaaagttat gtttactcaa
      121 cttaggaaat agaaatagat caatttatat tctcttgtca atcgaatatg aaccttagca
      181 ctatatacca aatatagtaa aatgctgaaa gatg
//
LOCUS       DACEUZ010000310          209 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.329, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000310 DACEUZ010000000
VERSION     DACEUZ010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..209
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.329"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            <1..>209
                     /locus_tag="IXG44_002700"
     CDS             <1..>209
                     /locus_tag="IXG44_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ1659374.1"
                     /translation="RWSVVTRLKKNTSMTEIAAQKNLSVSSVYRIMKRFYRPLNPFRT
                     PLPKVLCFDEFKSVRGVSGAMSFIM"
BASE COUNT           51 a           43 c           44 g           71 t
ORIGIN      
        1 cgctggtctg tcgtgactcg tctgaaaaaa aatacctcaa tgacagagat cgccgctcag
       61 aaaaatctat cggtctcttc tgtttaccgt attatgaagc gattttatcg tccgttgaat
      121 ccttttcgga caccgttacc taaggtactt tgttttgatg aattcaaatc agttcggggt
      181 gtttcaggtg ctatgagttt catcatgat
//
LOCUS       DACEUZ010000311          208 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.330, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000311 DACEUZ010000000
VERSION     DACEUZ010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..208
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.330"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            50..>208
                     /locus_tag="IXG44_002701"
     CDS             50..>208
                     /locus_tag="IXG44_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017370594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAQ1659375.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSH
                     TTIYRWVQE"
BASE COUNT           72 a           29 c           46 g           61 t
ORIGIN      
        1 actatcaaat aaggtagaat aatagaaaaa gatagcagga ggaatgacga tgaatcattt
       61 taaaggaaag caatttcagc aggatgtgat tattgtagcc gtgggctact atcttcgtta
      121 taaccttagc tatcgtgaag ttcaagaaat cttatatgat cgtggcatta acgtttctca
      181 tacgacgatt tatcgttggg tgcaagaa
//
LOCUS       DACEUZ010000312          205 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.331, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000312 DACEUZ010000000
VERSION     DACEUZ010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..205
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.331"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            complement(21..94)
                     /locus_tag="IXG44_002702"
     tRNA            complement(21..94)
                     /locus_tag="IXG44_002702"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58..60),aa:Ile,seq:cat)
     gene            complement(108..181)
                     /locus_tag="IXG44_002703"
     tRNA            complement(108..181)
                     /locus_tag="IXG44_002703"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(145..147),aa:Met,seq:cat)
BASE COUNT           59 a           57 c           49 g           40 t
ORIGIN      
        1 tgaaacttac aacgccacaa tggaccttgt aggactcgaa cctacgaccg gacggttatg
       61 agccgtctgc tctaaccaac tgagctaaag gtccaggctc ttaaaaatcg cggcggaggg
      121 gatcgaaccc ccgacctccc gggtatgaac cggacgctct agccagctga gctacaccgc
      181 gaaaaagata ttcattattt aaatg
//
LOCUS       DACEUZ010000313          203 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.332, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000313 DACEUZ010000000
VERSION     DACEUZ010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..203
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.332"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
BASE COUNT           73 a           37 c           41 g           52 t
ORIGIN      
        1 accccttcta gggggattaa aaatacaccc gtcctcacgg gtgtatgggt tactgataga
       61 cttgatttta actggtgtca gaaacttatg tccagaaagg gtgatgaaat tttggcaatt
      121 tacggctatt caagaattag cacaagcaac caagattaca aaacacaaat tcaaacatta
      181 gaaaatgctg gcgcagaaat aat
//
LOCUS       DACEUZ010000314          201 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.denovo.333, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000314 DACEUZ010000000
VERSION     DACEUZ010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..201
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.denovo.333"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            <1..>201
                     /locus_tag="IXG44_002704"
     CDS             <1..>201
                     /locus_tag="IXG44_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ1659376.1"
                     /translation="CQVSLTTVIRTLKEFKSYLPKQSKRILPRVLMVDEFRSHASIED
                     KMSFICADGETGQLIDVLPTRK"
BASE COUNT           60 a           37 c           42 g           62 t
ORIGIN      
        1 ttgtcaggta tctttgacca ccgttattcg tacgttgaaa gagtttaaaa gctacttacc
       61 aaagcaatct aagaggattc tccccagagt attgatggtt gatgaatttc gttcgcacgc
      121 ttccatagaa gataaaatga gctttatttg cgcagatggc gaaacaggac aattaattga
      181 tgtcttgcct acgcgtaaat t
//
LOCUS       DACEUZ010000315         2411 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.guided.192, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000315 DACEUZ010000000
VERSION     DACEUZ010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.guided.192"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            127..243
                     /locus_tag="IXG44_002705"
     CDS             127..243
                     /locus_tag="IXG44_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HAQ1659377.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            259..2178
                     /gene="tet(M)"
                     /locus_tag="IXG44_002706"
     CDS             259..2178
                     /gene="tet(M)"
                     /locus_tag="IXG44_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HAQ1659378.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            2276..>2411
                     /locus_tag="IXG44_002707"
     CDS             2276..>2411
                     /locus_tag="IXG44_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1659379.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     K"
BASE COUNT          816 a          360 c          492 g          743 t
ORIGIN      
        1 agttaataaa tatgcggcaa ggtattctta aataaactgt caatttgata gtgggaacaa
       61 ataattggat gtcctttttt aggagggctt agttttttgc acccagttta agaatacctt
      121 tatcatgtga ttctaaagta tccggagaat atctgtatgc tttgtatgcc tatggttatg
      181 cataaaaatc ccagtgataa gagtatttat cactgggatt tttatgccct tttgggcttt
      241 tgaatggagg aaaatcacat gaaaattatt aatattggag ttttagctca tgttgatgcg
      301 ggaaaaacta ccttaacaga aagcttatta tataacagtg gagcgattac agaattagga
      361 agcgtggaca aaggtacaac gaggacggat aatacgcttt tagaacgtca gagaggaatt
      421 acaattcaga caggaataac ctcttttcag tgggaaaata cgaaggtgaa catcatagac
      481 acgccaggac atatggattt cttagcagaa gtatatcgtt cattatcagt tttagatggg
      541 gcaattctac tgatttctgc aaaagatggc gtacaagcac aaactcgtat attatttcat
      601 gcacttagga aaatggggat tcccacaatc ttttttatca ataagattga ccaaaatgga
      661 attgatttat caacggttta tcaggatatt aaagagaaac tttctgccga aattgtaatc
      721 aaacagaagg tagaactgta tcctaatatg tgtgtgacga actttaccga atctgaacaa
      781 tgggatacgg taatagaggg aaacgatgac cttttagaga aatatatgtc cggtaaatca
      841 ttagaagcat tggaactcga acaagaggaa agcataagat ttcagaattg ctccttgtac
      901 cctgtttatc atggaagcgc aaaaagcaac atagggattg agcagcttat agaagtgata
      961 acgaataaat tttattcatc aacatacaga aagaagtctg aactttgcgg aaatgtcttc
     1021 aaaattgaat attcggaaga aagacaacgt cttgcatatg tacgccttta tggcggaatc
     1081 ctgcatttgc gggattcggt tagaatatcg gaaaaggaaa aaataaaaat tacagaaatg
     1141 tatacttcaa taaatggtga attatgtaaa attgataagg cttattccgg ggaaattgtt
     1201 attttgcaaa atgagttttt gaagctaaat agtgttcttg gagatacaaa gctattgcca
     1261 cagagagaga gaattgaaaa tccgctccct ctgctgcaaa caactgttga accgagcaaa
     1321 cctcaacaaa gggaaatgtt acttgatgca cttttagaaa tctccgacag tgacccgctt
     1381 ctacaatatt atgtggattc tacgacacat gaaattatac tttctttctt agggaaagta
     1441 caaatggaag tgattagtgc actgttgcaa gaaaagtatc atgtggagat agaactaaaa
     1501 gagcctacag tcatttatat ggagagaccg ttaaaaaatg cagaatatac cattcacatc
     1561 gaagtgccgc caaatccttt ctgggcttcc attggtttat ctgtatcacc gcttccgttg
     1621 ggaagtggaa tgcagtatga gagctcggtt tctcttggat acttaaatca atcatttcaa
     1681 aatgcagtta tggaagggat acgctatggt tgcgaacaag gattatatgg ttggaatgtg
     1741 acggattgta aaatctgttt taagtatggc ttatactata gccctgttag taccccagca
     1801 gattttcgga tgcttgctcc tattgtattg gaacaagtct taaaaaaagc tggaacagaa
     1861 ttgttagagc catatcttag ttttaaaatt tatgcgccac aggaatatct ttcacgagca
     1921 tacaacgatg ctcctaaata ttgtgcgaac atcgtagaca ctcaattgaa aaataatgag
     1981 gtcattctta gtggagaaat ccctgctcgg tgtattcaag aatatcgtag tgatttaact
     2041 ttctttacaa atggacgtag tgtttgttta acagagttaa aagggtacca tgttactacc
     2101 ggtgaacctg tttgccagcc ccgtcgtcca aatagtcgga tagataaagt acgatatatg
     2161 ttcaataaaa taacttagtg tattttatgt tgttatataa atatggtttc ttgttaaata
     2221 agatgaaata ttttttaata aagatttgaa ttaaagtgta aaggaggaat taattatgaa
     2281 gtgtaatgaa tgtaacaggg ttaaatacaa ccaaatttta atttggcttt gcgttttatc
     2341 tttttttagc gtattaaatg aaatggtttt gaacgtctca ttacctgata ttgcaaatga
     2401 ttttaataaa c
//
LOCUS       DACEUZ010000316         1855 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.guided.209, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000316 DACEUZ010000000
VERSION     DACEUZ010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1855
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.guided.209"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            2..1504
                     /locus_tag="IXG44_002708"
     CDS             2..1504
                     /locus_tag="IXG44_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAQ1659380.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            1571..>1855
                     /locus_tag="IXG44_002709"
     CDS             1571..>1855
                     /locus_tag="IXG44_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ1659381.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKD"
BASE COUNT          670 a          337 c          408 g          440 t
ORIGIN      
        1 aatgtctaaa atcgaaataa aaaatctgac attcggctac gacagccaag gcacattatt
       61 atttgaacaa gcaaatctaa attttgacac acaatggaaa ctaggactta tcggacgaaa
      121 cggtcgagga aagacaactt tactgaatat tctacaaaac aaactacctt atcaagggca
      181 agtaatccat cagcaagaat ttgcctattt cccgcaacag acaaaagata aagaacgttt
      241 aacctattac gtgttaaatg atattacgga ttttgagata tgggaaatcg aaagagagct
      301 ccaattgatg caaacagatc ctgaaatctt atggagagaa ttcagcacac tatcgggggg
      361 agagaagaca aaagtcctac tggcactttt atttgtggat gacactcatt tcccgttaat
      421 cgatgaacca acgaatcatt tggatatctc tggtagaaaa caagtagcgg cttatttgaa
      481 aaagaaaaaa caaggcttca tcgtggtcag ccatgaccgg ggatttatcg atgaagtagt
      541 ggaccatgtt ttagcaatcg aaaaaagtca actggaactt tatcaaggga atttctctat
      601 ctatgaagaa cagaaaaaac ttcgtgatga atttgaaatg gctcaaaatg aaaaattgaa
      661 aaaagaagtc agtaggctaa agaaaacagc agctgaaaaa gccgaatggt ctcgttcccg
      721 agaaggagat aaaacaaaga aacaagtcgg attcatcgat actgaatcta gacgagtgaa
      781 taaaggagca gtgggtgctg atgctgcacg gacgatgaaa cgatccaaag caatcgtgaa
      841 tcggatggag acccagatca gcgagaaaga aaaactatta aaagatatcg aatatatcga
      901 ttctttgacg atgaatagcc aagcgtctca ccataagcga cttttaagcg tagaagatct
      961 tcaattaggg tatgaaaatc tgttattcga gccaattcat tttacaatcg agcctcatca
     1021 gcgggtggcg atttcaggtc ctaacggtgc aggaaagtca tccattatcc attatcttct
     1081 gggggcattc aacggcaagg ttataggaga aaaataccag ccaaaacatc tgagcattag
     1141 ttatgcaagc caaaattatg aagacaatcg aggaacgttg gcggaatttg cagagaaaaa
     1201 ccaagtagac taccaagcat ttttgaacaa cctccgaaag cttgggatgg aaagagatgt
     1261 ttttcataac aagatcgagc agatgagtat gggccaacgg aaaaaagtgg aattggctaa
     1321 atctttatca cagccagctg aactatatac atgggatgaa ccattgaatt atttggatgt
     1381 cttcaatcaa gaacaattag aacaactgat cttgaacgtg aaacctgcca tgttactagt
     1441 ggaacatgat caaaccttcc tggataaagt atctactgag attatttctc ttgagagaat
     1501 ctaacccttg taatctcaag aacaacaagg tatggttaag gggaactttg ccaacgaagg
     1561 agagaatcac atgctttatg taacgagaca cggagaaaca acttggaacg cacaaggatt
     1621 agtttgcgga cgggcagatg tgccgctgac tgaaaaagga cagatgcagg cacagaaact
     1681 agcagaaaag gtagtagatt tgcctgttcc aatcacgaaa atcatccatt cccctcttca
     1741 acgagcaaga gatacagcac aagcagttgc tgatcgtctc agcttgccgc tgaccgttga
     1801 tgaacgattg gtagaaatgg attttgggga ttatgatgga ctgccaagca aagat
//
LOCUS       DACEUZ010000317         1825 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.guided.211, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000317 DACEUZ010000000
VERSION     DACEUZ010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1825
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.guided.211"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            85..1563
                     /gene="msr"
                     /locus_tag="IXG44_002710"
     CDS             85..1563
                     /gene="msr"
                     /locus_tag="IXG44_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAQ1659382.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            1610..>1825
                     /locus_tag="IXG44_002711"
     CDS             1610..>1825
                     /locus_tag="IXG44_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAQ1659383.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVN"
BASE COUNT          624 a          306 c          388 g          507 t
ORIGIN      
        1 atgcagtttt tttgcaaaca gatttttctg ctctttttta gtatcgataa ttgttgtttt
       61 acatgaaata ggagtgtaaa acatatggaa aatttagcag taaatataac aaatctacaa
      121 gtcagctttg gcaaccagct agaactatct attgattctc ttcgtgtcta tcagcaagat
      181 cgtataggga tcataggaga aaatggtgtc ggtaaatcga ctttgctcaa actaatagcc
      241 ggtgaacttt ttcccgatca tgggaaaatc caaacagaga tcaccttcaa ctacctgcct
      301 caattaacct atctcgctga agcaaaggac ctaaatttgg aattagccag tcatttccag
      361 ttaagactgg aagaaacttc agagcggaaa tggagcggag gggaagaacg aaagatcgag
      421 ttgatacgtc ttctttcttc ttatgaacaa gggatgcttc tagacgagcc aacaacccat
      481 ctagatagaa aaagtattga tcgactgatt gaagagcttc gttattatta tggtacgctg
      541 gtttttgtta gtcatgatcg ctattttcta gatgagctag catcgaaaat ctgggaagta
      601 aaagacggag aaatccgaga gttttcgggg aattatagtg cctatctcac tcaaaaggaa
      661 ttggagaaaa agactcagct acgagaagca gagtcgatca tgaaagagaa aaaacgattg
      721 gaaaaatcga tccaagaaaa gaaaaaacaa gcggaaaagt tggaaaaagt gtccagtaaa
      781 aagaaaaagc aacaaatcag accggatcgg ttgtcttcct ctaaacaaaa agacagtgta
      841 caaaaagcca tccaaaagaa tgcgaaaaca ttagagagaa gactccaaaa aataggagaa
      901 acaaccaagc cgcagcagat gaaacaaatc cgttttccag taccaaaatc tcttgaactc
      961 cacagccgct atcccatcat gggacaaaat gtccaattgg aaagaagtgg gagaacatta
     1021 ctggtaaatg gtgattttca gttttcttta ggtaaaaaaa tcgcgattgt cggtgaaaat
     1081 ggttcaggta agacaacctt attagaacat atccgcaaac aaggagaagg aatccttctc
     1141 tctccgaaag taagctttca agtatatcag caaaagggtt atcaaatgac atctgaagaa
     1201 tccatcattc gttttgtcat gagacaaaca gagttttcag aatcgcttgt ccgtagtttg
     1261 ctgaatcact tagggtttgc tcaggaaact ttgacgaaac cgttatgtac attaagtggg
     1321 ggagaagcga cccgtctgac gattgctttg ctttttacta agccaagtaa tgtgttgctg
     1381 ttagatgaac cgactaattt tattgatatg gcaacgatcg aagctttaga gaagctaatg
     1441 caaatatatc cgggaacgat tttgtttact tctcatgatt cttactttgt cgagcgtacg
     1501 gctgatgaag tttatgaaat aaaagggcag aaaataaaaa aagtacttac gagaaatttt
     1561 taaatgtgct acgatgattt tcgtgacaaa tttaaaggag tggtacagat tgcctacaaa
     1621 taaaaaagaa ggaataattt ttacaacaat gatgtgtttt ttgatggtct tggggatgag
     1681 cgcgtataat ttatggctgc atcaagatct tcatttatca agtttgttga ttggtctagt
     1741 gcctggattc gtggtggctt tcattttgga tgtcttcatc atcggtgtgc tggcgaaaaa
     1801 aattgccttt aaactaccag taaat
//
LOCUS       DACEUZ010000318         1492 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.guided.224, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000318 DACEUZ010000000
VERSION     DACEUZ010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1492
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.guided.224"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            246..1214
                     /locus_tag="IXG44_002712"
     CDS             246..1214
                     /locus_tag="IXG44_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HAQ1659384.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            1207..>1492
                     /locus_tag="IXG44_002713"
     CDS             1207..>1492
                     /locus_tag="IXG44_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HAQ1659385.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAF"
BASE COUNT          462 a          278 c          347 g          405 t
ORIGIN      
        1 gatgctggtg tcatccacca acatcaaaaa gtatagagcc ataatttttt aggaaaatct
       61 caaggttatc tttacttttt cttaggaaat taacaattta atattaagaa acggctcgtt
      121 cttacacggt agacttaata ccgtaagaac gagccgtttt cgttcttcag agaaagattt
      181 gacaagatta ccattggcat ccccgtttta tttggtgcct ttcacagaaa gggttggtct
      241 taattatgaa taacatcggc attactgttt atggatgtga gcaggatgag gcagatgcat
      301 tccatgctct ttcgcctcgc tttggcgtta tggcaacgat aattaacgcc aacgtgtcgg
      361 aatccaacgc caaatccgcg cctttcaatc aatgtatcag tgtgggacat aaatcagaga
      421 tttccgcctc tattcttctt gcgctgaaga gagccggtgt gaaatatatt tctacccgaa
      481 gcatcggctg caatcatata gatacaactg ctgctaagag aatgggcatc actgtcgaca
      541 atgtggcgta ctcgccggat agcgttgccg attatactat gatgctaatt cttatggcag
      601 tacgcaacgt aaaatcgatt gtgcgctctg tggaaaaaca tgatttcagg ttggacagcg
      661 accgtggcaa ggtactcagc gacatgacag ttggtgtggt gggaacgggc cagataggca
      721 aagcggttat tgagcggctg cgaggatttg gatgtaaagt gttggcttat agtcgcagcc
      781 gaagtataga ggtaaactat gtaccgtttg atgagttgct gcaaaatagc gatatcgtta
      841 cgcttcatgt gccgctcaat acggatacgc actatattat cagccacgaa caaatacaga
      901 gaatgaagca aggagcattt cttatcaata ctgggcgcgg tccacttgta gatacctatg
      961 agttggttaa agcattagaa aacgggaaac tgggcggtgc cgcattggat gtattggaag
     1021 gagaggaaga gtttttctac tctgattgca cccaaaaacc aattgataat caatttttac
     1081 ttaaacttca aagaatgcct aacgtgataa tcacaccgca tacggcctat tataccgagc
     1141 aagcgttgcg tgataccgtt gaaaaaacca ttaaaaactg tttggatttt gaaaggagac
     1201 aggagcatga atagaataaa agttgcaata ctgtttgggg gttgctcaga ggagcatgac
     1261 gtatcggtaa aatctgcaat agagatagcc gctaacatta ataaagaaaa atacgagccg
     1321 ttatacattg gaattacgaa atctggtgta tggaaaatgt gcgaaaaacc ttgcgcggaa
     1381 tgggaaaacg acaattgcta ttcagctgta ctctcgccgg ataaaaaaat gcacggatta
     1441 cttgttaaaa agaaccatga atatgaaatc aaccatgttg atgtagcatt tt
//
LOCUS       DACEUZ010000319         1480 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.guided.225, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000319 DACEUZ010000000
VERSION     DACEUZ010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1480
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.guided.225"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            18..926
                     /locus_tag="IXG44_002714"
     CDS             18..926
                     /locus_tag="IXG44_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HAQ1659386.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            923..1465
                     /gene="sat4"
                     /locus_tag="IXG44_002715"
     CDS             923..1465
                     /gene="sat4"
                     /locus_tag="IXG44_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HAQ1659387.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
BASE COUNT          522 a          215 c          329 g          414 t
ORIGIN      
        1 aaacgaggag tatgtaaatg agatcagaaa aagaaatgat ggatttagta ctttctttag
       61 cagaacagga tgaacgtatt cgaattgtga cccttgaggg gtcacgcgca aatattaata
      121 tacctaaaga tgaatttcag gattatgata ttacatattt tgtaagtgat atagaaccgt
      181 ttatatctaa tgatgactgg cttaatcaat ttgggaatat aataatgatg caaaagccgg
      241 aggatatgga attattccca cctgaagaaa agggattttc ctatcttatg ctatttgatg
      301 attacaataa aattgatctt accttattgc ccttggaaga gttagataat tacctaaagg
      361 gcgataaatt aataaaggtt ctaattgata aagattgtag aattaaaagg gacatagttc
      421 cgactgatat agattatcat gtaagaaagc caagcgcaag ggagtatgat gattgctgca
      481 atgaattttg gaatgtaaca ccttatgtta ttaaaggatt gtgccgtaag gaaattttat
      541 ttgctattga tcattttaat cagattgttc gccatgagct gctgagaatg atatcatgga
      601 aggtcggcat cgaaacaggc tttaaattaa gtgtaggcaa gaactataag tttattgaaa
      661 ggtatatatc cgaggatttg tgggagaaac ttttgtccac ctaccggatg gattcctatg
      721 aaaacatatg ggaagcatta tttctatgcc atcaattgtt cagggcggta tccggtgagg
      781 tggcggaaag gcttcattat gcctatccgg agtatgatag gaatataaca aaatatacca
      841 gggacatgta taaaaaatac actggtaaaa ccggctgcct ggatagcaca tatgccgctg
      901 atatagaaga gaggcgggaa cagtgattac agaaatgaaa gcagggcacc tgaaagatat
      961 cgataaaccc agcgaaccat ttgaggtgat aggtaagatt ataccgaggt atgaaaacga
     1021 gaattggacc tttacagaat tactctatga agcgccatat ttaaaaagct accaagacga
     1081 agaggatgaa gaggatgagg aggcagattg ccttgaatat attgacaata ctgataagat
     1141 aatatatctt tactaccaag acgataaatg cgtcggaaaa gttaaactgc gaaaaaattg
     1201 gaaccggtac gcttatatag aagatatcgc cgtatgtaag gatttcaggg ggcaaggcat
     1261 aggcagcgcg cttatcaata tatctataga atgggcaaag cataaaaact tgcatggact
     1321 aatgcttgaa acccaggaca ataaccttat agcttgtaaa ttctatcata attgtggttt
     1381 caaaatcggc tccgtcgata ctatgttata cgccaacttt gaaaacaact ttgaaaaagc
     1441 tgttttctgg tatttaaggt tttagaatgc aaggaacagt
//
LOCUS       DACEUZ010000320         1405 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.guided.231, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000320 DACEUZ010000000
VERSION     DACEUZ010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1405
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.guided.231"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            <1..307
                     /locus_tag="IXG44_002716"
     CDS             <1..307
                     /locus_tag="IXG44_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016204422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1659388.1"
                     /translation="LKDTWLDLSEDNPYSQFLITVMAGVNQLERDLIRMRQREGIELA
                     KKEGKFKGRLKKYHKNHAGMNYAVKLYKEGNMTVNQICEITNVSRASLYRKLSEVNN"
     gene            521..1216
                     /gene="vanR"
                     /locus_tag="IXG44_002717"
     CDS             521..1216
                     /gene="vanR"
                     /locus_tag="IXG44_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HAQ1659389.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1194..>1405
                     /locus_tag="IXG44_002718"
     CDS             1194..>1405
                     /locus_tag="IXG44_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAQ1659390.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYS"
BASE COUNT          505 a          231 c          285 g          384 t
ORIGIN      
        1 actaaaagat acatggcttg atttatcaga agataatcca tacagccaat tcttaattac
       61 tgtaatggct ggtgttaacc aattagagcg agatcttatt cggatgagac aacgtgaagg
      121 gattgaattg gctaagaaag aaggaaagtt taaaggtcga ttaaagaagt atcataaaaa
      181 tcacgcagga atgaattatg cggtaaagct atataaagaa ggaaatatga ctgtaaatca
      241 aatttgtgaa attactaatg tatctagggc ttcattatac aggaaattat cagaagtgaa
      301 taattagcca ttctgtattc cgctaatggg caatattttt aaagaagaaa aggaaactat
      361 aaaatattaa cagcctccta gcgatgccga aaagcccttt gataaaaaaa gaatcatcat
      421 cttaagaaat tcttagtcat ttattatgta aatgcttata aattcggccc tataatctga
      481 taaattatta agggcaaact tatgtgaaag ggtgataact atgagcgata aaatacttat
      541 tgtggatgat gaacatgaaa ttgccgattt ggttgaatta tacttaaaaa acgagaatta
      601 tacggttttc aaatactata ccgccaaaga agcattggaa tgtatagaca agtctgagat
      661 tgaccttgcc atattggaca tcatgcttcc cggcacaagc ggccttacta tctgtcaaaa
      721 aataagggac aagcacacct atccgattat catgctgacc gggaaagata cagaggtaga
      781 taaaattaca gggttaacaa tcggcgcgga tgattatata acgaagccct ttcgcccact
      841 ggagttaatt gctcgggtaa aggcccagtt gcgccgatac aaaaaattca gtggagtaaa
      901 ggagcagaac gaaaatgtta tcgtccactc cggccttgtc attaatgtta acacccatga
      961 gtgttatctg aacgagaagc agttatccct tactcccacc gagttttcaa tactgcgaat
     1021 cctctgtgaa aacaagggga atgtggttag ctccgagctg ctatttcatg agatatgggg
     1081 cgacgaatat ttcagcaaga gcaacaacac catcaccgtg catatccggc atttgcgcga
     1141 aaaaatgaac gacaccattg ataatccgaa atatataaaa acggtatggg gggttggtta
     1201 taaaattgaa aaataaaaaa aacgactatt ccaaactaga acgaaaactt tacatgtata
     1261 tcgttgcaat tgttgtggta gcaattgtat tcgtgttgta tattcgttca atgatccgag
     1321 ggaaacttgg ggattggatc ttaagtattt tggaaaacaa atatgactta aatcacctgg
     1381 acgcgatgaa attatatcaa tattc
//
LOCUS       DACEUZ010000321         1292 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.guided.235, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000321 DACEUZ010000000
VERSION     DACEUZ010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1292
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.guided.235"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            294..1205
                     /gene="vanY"
                     /locus_tag="IXG44_002719"
     CDS             294..1205
                     /gene="vanY"
                     /locus_tag="IXG44_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HAQ1659391.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFYQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYELWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
BASE COUNT          456 a          189 c          271 g          376 t
ORIGIN      
        1 attatctgcg tgcgctgcgg caagatagcc tgataataag actgatcgca tagaggggtg
       61 gtatttcaca ccgcccattg tcaacaggca gttcagcctc gttaaattca gcatgggtat
      121 cacttatgaa aattcatcta cattggtgat aatagtaaat ccagtagggc gaaataattg
      181 actgtaattt acggggcaaa acggcacaat ctcaaacgag attgtgccgt ttaaggggaa
      241 gattctagaa atatttcata cttccaacta tatagttaag gaggagactg aaaatgaaga
      301 agttgttttt tttattgtta ttgttattct taatatactt aggttatgac tacgttaatg
      361 aagcactgtt ttatcaggaa aaagtcgaat ttcaaaatta tgatcaaaat cccaaagaac
      421 atttagaaaa tagtgggact tctgaaaata cccaagagaa aacaattaca gaagaacagg
      481 tttatcaagg aaatctgcta ttaatcaata gtaaatatcc tgttcgccaa gaaagtgtga
      541 agtcagatat cgtgaattta tctaaacatg acgaattaat aaatggatac gggttgcttg
      601 atagtaatat ttatatgtca aaagaaatag cacaaaaatt ttcagagatg gtcaatgatg
      661 ctgtaaaggg tggcgttagt cattttatta ttaatagtgg ctatcgagac tttgatgagc
      721 aaagtgtgct ttaccaagaa atgggggctg agtatgcctt accagcaggt tatagtgagc
      781 ataattcagg tttatcacta gatgtaggat caagcttgac gaaaatggaa cgagcccctg
      841 aaggaaagtg gatagaagaa aatgcttgga aatacgggtt cattttacgt tatccagagg
      901 acaaaacaga gttaacagga attcaatatg aactatggca tattcgctat gttggtttac
      961 cacatagtgc gattatgaaa gaaaagaatt tcgttctcga ggaatatatg gattacctaa
     1021 aagaagaaaa aaccatttct gttagtgtaa atggggaaaa atatgagatc ttttattatc
     1081 ctgttactaa aaataccacc attcatgtgc cgactaatct tcgttatgag atatcaggaa
     1141 acaatataga cggtgtaatt gtgacagtgt ttcccggatc aacacatact aattcaagga
     1201 ggtaaggatg gcggaatgaa accaacgaaa ttaatgaaca gcattattgt actagcactt
     1261 ttggggtaac gttagctttt taatttaaaa cc
//
LOCUS       DACEUZ010000322         1292 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.guided.236, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000322 DACEUZ010000000
VERSION     DACEUZ010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1292
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.guided.236"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            294..1205
                     /gene="vanY"
                     /locus_tag="IXG44_002720"
     CDS             294..1205
                     /gene="vanY"
                     /locus_tag="IXG44_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HAQ1659392.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSKEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYELWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
BASE COUNT          456 a          189 c          271 g          376 t
ORIGIN      
        1 attatctgcg tgcgctgcgg caagatagcc tgataataag actgatcgca tagaggggtg
       61 gtatttcaca ccgcccattg tcaacaggca gttcagcctc gttaaattca gcatgggtat
      121 cacttatgaa aattcatcta cattggtgat aatagtaaat ccagtagggc gaaataattg
      181 actgtaattt acggggcaaa acggcacaat ctcaaacgag attgtgccgt ttaaggggaa
      241 gattctagaa atatttcata cttccaacta tatagttaag gaggagactg aaaatgaaga
      301 agttgttttt tttattgtta ttgttattct taatatactt aggttatgac tacgttaatg
      361 aagcactgtt ttctaaggaa aaagtcgaat ttcaaaatta tgatcaaaat cccaaagaac
      421 atttagaaaa tagtgggact tctgaaaata cccaagagaa aacaattaca gaagaacagg
      481 tttatcaagg aaatctgcta ttaatcaata gtaaatatcc tgttcgccaa gaaagtgtga
      541 agtcagatat cgtgaattta tctaaacatg acgaattaat aaatggatac gggttgcttg
      601 atagtaatat ttatatgtca aaagaaatag cacaaaaatt ttcagagatg gtcaatgatg
      661 ctgtaaaggg tggcgttagt cattttatta ttaatagtgg ctatcgagac tttgatgagc
      721 aaagtgtgct ttaccaagaa atgggggctg agtatgcctt accagcaggt tatagtgagc
      781 ataattcagg tttatcacta gatgtaggat caagcttgac gaaaatggaa cgagcccctg
      841 aaggaaagtg gatagaagaa aatgcttgga aatacgggtt cattttacgt tatccagagg
      901 acaaaacaga gttaacagga attcaatatg aactatggca tattcgctat gttggtttac
      961 cacatagtgc gattatgaaa gaaaagaatt tcgttctcga ggaatatatg gattacctaa
     1021 aagaagaaaa aaccatttct gttagtgtaa atggggaaaa atatgagatc ttttattatc
     1081 ctgttactaa aaataccacc attcatgtgc cgactaatct tcgttatgag atatcaggaa
     1141 acaatataga cggtgtaatt gtgacagtgt ttcccggatc aacacatact aattcaagga
     1201 ggtaaggatg gcggaatgaa accaacgaaa ttaatgaaca gcattattgt actagcactt
     1261 ttggggtaac gttagctttt taatttaaaa cc
//
LOCUS       DACEUZ010000323         1292 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.guided.237, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000323 DACEUZ010000000
VERSION     DACEUZ010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1292
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.guided.237"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            294..1205
                     /gene="vanY"
                     /locus_tag="IXG44_002721"
     CDS             294..1205
                     /gene="vanY"
                     /locus_tag="IXG44_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HAQ1659393.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYELWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
BASE COUNT          455 a          190 c          271 g          376 t
ORIGIN      
        1 attatctgcg tgcgctgcgg caagatagcc tgataataag actgatcgca tagaggggtg
       61 gtatttcaca ccgcccattg tcaacaggca gttcagcctc gttaaattca gcatgggtat
      121 cacttatgaa aattcatcta cattggtgat aatagtaaat ccagtagggc gaaataattg
      181 actgtaattt acggggcaaa acggcacaat ctcaaacgag attgtgccgt ttaaggggaa
      241 gattctagaa atatttcata cttccaacta tatagttaag gaggagactg aaaatgaaga
      301 agttgttttt tttattgtta ttgttattct taatatactt aggttatgac tacgttaatg
      361 aagcactgtt ttctcaggaa aaagtcgaat ttcaaaatta tgatcaaaat cccaaagaac
      421 atttagaaaa tagtgggact tctgaaaata cccaagagaa aacaattaca gaagaacagg
      481 tttatcaagg aaatctgcta ttaatcaata gtaaatatcc tgttcgccaa gaaagtgtga
      541 agtcagatat cgtgaattta tctaaacatg acgaattaat aaatggatac gggttgcttg
      601 atagtaatat ttatatgtca aaagaaatag cacaaaaatt ttcagagatg gtcaatgatg
      661 ctgtaaaggg tggcgttagt cattttatta ttaatagtgg ctatcgagac tttgatgagc
      721 aaagtgtgct ttaccaagaa atgggggctg agtatgcctt accagcaggt tatagtgagc
      781 ataattcagg tttatcacta gatgtaggat caagcttgac gaaaatggaa cgagcccctg
      841 aaggaaagtg gatagaagaa aatgcttgga aatacgggtt cattttacgt tatccagagg
      901 acaaaacaga gttaacagga attcaatatg aactatggca tattcgctat gttggtttac
      961 cacatagtgc gattatgaaa gaaaagaatt tcgttctcga ggaatatatg gattacctaa
     1021 aagaagaaaa aaccatttct gttagtgtaa atggggaaaa atatgagatc ttttattatc
     1081 ctgttactaa aaataccacc attcatgtgc cgactaatct tcgttatgag atatcaggaa
     1141 acaatataga cggtgtaatt gtgacagtgt ttcccggatc aacacatact aattcaagga
     1201 ggtaaggatg gcggaatgaa accaacgaaa ttaatgaaca gcattattgt actagcactt
     1261 ttggggtaac gttagctttt taatttaaaa cc
//
LOCUS       DACEUZ010000324         1252 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.guided.239, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000324 DACEUZ010000000
VERSION     DACEUZ010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1252
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.guided.239"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            43..1197
                     /locus_tag="IXG44_002722"
     CDS             43..1197
                     /locus_tag="IXG44_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAQ1659394.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
BASE COUNT          409 a          231 c          277 g          335 t
ORIGIN      
        1 acaccattga taatccgaaa tatataaaaa cggtatgggg ggttggttat aaaattgaaa
       61 aataaaaaaa acgactattc caaactagaa cgaaaacttt acatgtatat cgttgcaatt
      121 gttgtggtag caattgtatt cgtgttgtat attcgttcaa tgatccgagg gaaacttggg
      181 gattggatct taagtatttt ggaaaacaaa tatgacttaa atcacctgga cgcgatgaaa
      241 ttatatcaat attccatacg gaacaatata gatatcttta tttatgtggc gattgtcatt
      301 agtattctta ttctatgtcg cgtcatgctt tcaaaattcg caaaatactt tgacgagata
      361 aataccggca ttgatgtact tattcagaac gaagataaac aaattgagct ttctgcggaa
      421 atggatgtta tggaacaaaa gctcaacaca ttaaaacgga ctctggaaaa gcgagagcag
      481 gatgcaaagc tggccgaaca aagaaaaaat gacgttgtta tgtacttggc gcacgatatt
      541 aaaacgcccc ttacatccat tatcggttat ttgagcctgc ttgacgaggc tccagacatg
      601 ccggtagatc aaaaggcaaa gtatgtgcat atcacgttgg acaaagcgta tcgactcgaa
      661 cagctaatcg acgagttttt tgagattaca cggtataacc tacaaacgat aacgctaaca
      721 aaaacgcaca tagacctata ctatatgctg gtgcagatga ccgatgaatt ttatcctcag
      781 ctttccgcac atggaaaaca ggcggttatt cacgcccccg aggatctgac cgtgtccggc
      841 gaccctgata aactcgcgag agtctttaac aacattttga aaaacgccgc tgcatacagt
      901 gaggataaca gcatcattga cattaccgcg ggcctctccg gggatgtggt gtcaatcgaa
      961 ttcaagaaca ctggaagcat cccaaaagat aagctagctg ccatatttga aaagttctat
     1021 aggctggaca atgctcgttc ttccgatacg ggtggcgcgg gacttggatt ggcgattgca
     1081 aaagaaatta ttgttcagca tggagggcag atttacgcgg aaagcaatga taactatacg
     1141 acgtttaggg tagagcttcc agcgatgcca gacttggttg ataaaaggag gtcctaagag
     1201 atgtatataa ttttggctct ttgtcaaatg atgttggtaa agagaaatct ta
//
LOCUS       DACEUZ010000325         1176 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.guided.242, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000325 DACEUZ010000000
VERSION     DACEUZ010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1176
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.guided.242"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            <1..102
                     /locus_tag="IXG44_002723"
     CDS             <1..102
                     /locus_tag="IXG44_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HAQ1659395.1"
                     /translation="TPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            95..1126
                     /gene="vanA"
                     /locus_tag="IXG44_002724"
     CDS             95..1126
                     /gene="vanA"
                     /locus_tag="IXG44_002724"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HAQ1659396.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALAVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
BASE COUNT          352 a          221 c          297 g          306 t
ORIGIN      
        1 acaccgcata cggcctatta taccgagcaa gcgttgcgtg ataccgttga aaaaaccatt
       61 aaaaactgtt tggattttga aaggagacag gagcatgaat agaataaaag ttgcaatact
      121 gtttgggggt tgctcagagg agcatgacgt atcggtaaaa tctgcaatag agatagccgc
      181 taacattaat aaagaaaaat acgagccgtt atacattgga attacgaaat ctggtgtatg
      241 gaaaatgtgc gaaaaacctt gcgcggaatg ggaaaacgac aattgctatt cagctgtact
      301 ctcgccggat aaaaaaatgc acggattact tgttaaaaag aaccatgaat atgaaatcaa
      361 ccatgttgat gtagcatttt cagctttgca tggcaagtca ggtgaagatg gatccataca
      421 aggtctgttt gaattgtccg gtatcccttt tgtaggctgc gatattcaaa gctcagcaat
      481 ttgtatggac aaatcgttga catacatcgt tgcgaaaaat gctgggatag ctactcccgc
      541 cttttgggtt attaataaag atgataggcc ggtggcagct acgtttacct atcctgtttt
      601 tgttaagccg gcgcgttcag gctcatcctt cggtgtgaaa aaagtcaata gcgcggacga
      661 attggactac gcaattgaat cggcaagaca atatgacagc aaaatcttaa ttgagcaggc
      721 tgtttcgggc tgtgaggtcg gttgtgcggt attgggaaac agtgccgcgt tagctgttgg
      781 cgaggtggac caaatcaggc tgcagtacgg aatctttcgt attcatcagg aagtcgagcc
      841 ggaaaaaggc tctgaaaacg cagttataac cgttcccgca gacctttcag cagaggagcg
      901 aggacggata caggaaacgg caaaaaaaat atataaagcg ctcggctgta gaggtctagc
      961 ccgtgtggat atgtttttac aagataacgg ccgcattgta ctgaacgaag tcaatactct
     1021 gcccggtttc acgtcataca gtcgttatcc ccgtatgatg gccgctgcag gtattgcact
     1081 tcccgaactg attgaccgct tgatcgtatt agcgttaaag gggtgataag catggaaata
     1141 ggatttactt ttttagatga aatagtacac ggtgtt
//
LOCUS       DACEUZ010000326         1161 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.guided.243, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000326 DACEUZ010000000
VERSION     DACEUZ010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1161
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.guided.243"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            <1..1157
                     /gene="tet"
                     /locus_tag="IXG44_002725"
     CDS             <1..1157
                     /gene="tet"
                     /locus_tag="IXG44_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001574277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Tet(L)/Tet(K)/Tet(45) family tetracycline efflux
                     MFS transporter"
                     /protein_id="HAQ1659397.1"
                     /translation="QLGIKRLLLFGIIINCFGSVIGFVGHSFFSLLIMARFIQGAGAA
                     AFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGMIAHYIHWSYLLLIPM
                     ITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTTSYSISFLIVSVLSFL
                     IFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVSMVPYMMKDVHQLSTA
                     EIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSVSFLTASFLLETTSWF
                     MTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTSFLSEGTGIAIVGGLL
                     SIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVYKHSQRDF"
BASE COUNT          316 a          177 c          238 g          430 t
ORIGIN      
        1 atcaattagg catcaaaagg ttactcctat ttggaattat aataaattgt ttcgggtcgg
       61 taattgggtt tgttggccat tctttctttt ccttacttat tatggctcgt tttattcaag
      121 gggctggtgc agctgcattt ccagcactcg taatggttgt agttgcgcgc tatattccaa
      181 aggaaaatag gggtaaagca tttggtctta ttggatcgat agtagccatg ggagaaggag
      241 tcggtccagc gattggtgga atgatagccc attatattca ttggtcctat cttctactca
      301 ttcctatgat aacaattatc actgttccgt ttcttatgaa attattaaag aaagaagtaa
      361 ggataaaagg tcattttgat atcaaaggaa ttatactaat gtctgtaggc attgtatttt
      421 ttatgttgtt tacaacatca tatagcattt cttttcttat cgttagcgtg ctgtcattcc
      481 tgatatttgt aaaacatatc aggaaagtaa cagatccttt tgttgatccc ggattaggga
      541 aaaatatacc ttttatgatt ggagttcttt gtgggggaat tatatttgga acagtagcag
      601 ggtttgtctc tatggttcct tatatgatga aagatgttca ccagctaagt actgccgaaa
      661 tcggaagtgt aattattttc cctggaacaa tgagtgtcat tattttcggc tacattggtg
      721 ggatacttgt tgatagaaga ggtcctttat acgtgttaaa catcggagtt acatttcttt
      781 ctgttagctt tttaactgct tcctttcttt tagaaacaac atcatggttc atgacaatta
      841 taatcgtatt tgttttaggt gggctttcgt tcaccaaaac agttatatca acaattgttt
      901 caagtagctt gaaacagcag gaagctggtg ctggaatgag tttgcttaac tttaccagct
      961 ttttatcaga gggaacaggt attgcaattg taggtggttt attatccata cccttacttg
     1021 atcaaaggtt gttacctatg gaagttgatc agtcaactta tctgtatagt aatttgttat
     1081 tacttttttc aggaatcatt gtcattagtt ggctggttac cttgaatgta tataaacatt
     1141 ctcaaaggga tttctaaatc g
//
LOCUS       DACEUZ010000327         1047 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.guided.248, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000327 DACEUZ010000000
VERSION     DACEUZ010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1047
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.guided.248"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            155..892
                     /gene="erm(B)"
                     /locus_tag="IXG44_002726"
     CDS             155..892
                     /gene="erm(B)"
                     /locus_tag="IXG44_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAQ1659398.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNIRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          390 a          177 c          167 g          313 t
ORIGIN      
        1 actaaaaaca gtgattacgc agataaataa atacgttaga ttaattccta ccagtgacta
       61 atcttatgac tttttaaaca gataactaaa attacaaaca aatcgtttaa cttctgtatt
      121 tatttacaga tgtaataact tcaggagtga ttacatgaac aaaaatataa aatattctca
      181 aaacttttta acgagtgaaa aagtactcaa ccaaataata aaacaattga atttaaaaga
      241 aaccgatacc gtttacgaaa ttggaacagg taaagggcat ttaacgacga aactggctaa
      301 aataagtaaa caggtaacgt ctattgaatt agacagtcat ctattcaact tatcgtcaga
      361 aaaattaaaa ctgaacattc gtgtcacttt aattcaccaa gatattctac agtttcaatt
      421 ccctaacaaa cagaggtata aaattgttgg gagtattcct taccatttaa gcacacaaat
      481 tattaaaaaa gtggtttttg aaagccatgc gtctgacatc tatctgattg ttgaagaagg
      541 attctacaag cgtaccttgg atattcaccg aacactaggg ttgctcttgc acactcaagt
      601 ctcgattcag caattgctta agctgccagc ggaatgcttt catcctaaac caaaagtaaa
      661 cagtgtctta ataaaactta cccgccatac cacagatgtt ccagataaat attggaagct
      721 atatacgtac tttgtttcaa aatgggtcaa tcgagaatat cgtcaactgt ttactaaaaa
      781 tcagtttcat caagcaatga aacacgccaa agtaaacaat ttaagtaccg ttacttatga
      841 gcaagtattg tctattttta atagttatct attatttaac gggaggaaat aattctatga
      901 gtcgcttttt taaatttgga aagttacacg ttactaaagg gaatggagat aaattattag
      961 atatactact gacagcttcc aagaagctaa agaggtccct agcgcctacg gggaatttgg
     1021 gctctataat ttatgggact gatgaat
//
LOCUS       DACEUZ010000328         1000 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.guided.251, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000328 DACEUZ010000000
VERSION     DACEUZ010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1000
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.guided.251"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            <1..173
                     /locus_tag="IXG44_002727"
     CDS             <1..173
                     /locus_tag="IXG44_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAQ1659399.1"
                     /translation="KTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
     gene            500..997
                     /gene="dfrG"
                     /locus_tag="IXG44_002728"
     CDS             500..997
                     /gene="dfrG"
                     /locus_tag="IXG44_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HAQ1659400.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
BASE COUNT          410 a          110 c          185 g          295 t
ORIGIN      
        1 aaaaaacaaa tgtagaaact cagttaacag atacacaaaa gaaattagac gaagcaactg
       61 ctgaaaactc aagcttgaaa caatatattc aaaagttaga aaaagcaaaa gatgaagtgg
      121 aagatacagc tgataaatct cagcaaattg ttgaagaaca tttgcgtaaa taatctttct
      181 taacaagtaa acctaaatta gtaaagaagc aacagtaatc tatactaagt agttcagttt
      241 tggagtacaa aatgacaaaa tgtacttcaa aatggactac ttttttcatt ttaacttaca
      301 taccaaatta catacaaaat gatttataaa atgcaattga aaatgataat tgaatgaaaa
      361 tttgtgattt tgtggtatta tgataatata acttatagaa aaaattgctt attcaactaa
      421 cgggcaggat aatgagaaaa ggttttttga tagaaataag aaattagatg gtaaattagt
      481 taaaaaggct gtgttataaa tgaaagtttc tttgattgct gcgatggata agaatagagt
      541 gattggcaaa gagaatgaca ttccttggag gattcccaag gactgggaat atgttaaaaa
      601 tactacaaag ggacatccga taatattagg taggaagaac cttgaatcaa tcggaagagc
      661 cttacctgac agaagaaata ttattctgac gagagataag gggtttacct ttaatggttg
      721 tgaaattgtt cattcaatag aagatgtttt tgagttatgt aaaaacgaag aagaaatttt
      781 tattttcgga ggagaacaga tttataattt gtttttccct tatgttgaga aaatgtacat
      841 cacaaaaata catcatgaat tcgaaggaga tacttttttt ccagaagtga attatgagga
      901 atggaatgag gtatttgccc aaaaagggat aaagaatgat aaaaatccgt ataactacta
      961 ttttcatgta tatgaaagaa aaaacttatt gagttaacgg
//
LOCUS       DACEUZ010000329          899 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.guided.257, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000329 DACEUZ010000000
VERSION     DACEUZ010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..899
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.guided.257"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            <1..212
                     /locus_tag="IXG44_002729"
     CDS             <1..212
                     /locus_tag="IXG44_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HAQ1659401.1"
                     /translation="TAKKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRY
                     PRMMAAAGIALPELIDRLIVLALKG"
     gene            218..826
                     /locus_tag="IXG44_002730"
     CDS             218..826
                     /locus_tag="IXG44_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HAQ1659402.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT          263 a          182 c          214 g          240 t
ORIGIN      
        1 aaacggcaaa aaaaatatat aaagcgctcg gctgtagagg tctagcccgt gtggatatgt
       61 ttttacaaga taacggccgc attgtactga acgaagtcaa tactctgccc ggtttcacgt
      121 catacagtcg ttatccccgt atgatggccg ctgcaggtat tgcacttccc gaactgattg
      181 accgcttgat cgtattagcg ttaaaggggt gataagcatg gaaataggat ttactttttt
      241 agatgaaata gtacacggtg ttcgttggga cgctaaatat gccacttggg ataatttcac
      301 cggaaaaccg gttgacggtt atgaagtaaa tcgcattgta gggacatacg agttggctga
      361 atcgcttttg aaggcaaaag aactggctgc tacccaaggg tacggattgc ttctatggga
      421 cggttaccgt cctaatcgtg ctgtaaactg ttttatgcaa tgggctgcac agccggaaaa
      481 taacctgaca aaggaaagtt attatcccaa tattgaccga actgagatga tttcaaaagg
      541 atacgtggct tcaaaatcaa gccatagccg cggcagtgcc attgatctta cgctttatcg
      601 attagacacg ggtgagcttg taccaatggg gagccgattt gattttatgg atgaacgctc
      661 tcatcatgcg gcaaatggaa tatcatgcaa tgaagcgcaa aatcgcagac gtttgcgctc
      721 catcatggaa aacagtgggt ttgaagcata tagcctcgaa tggtggcact atgtattaag
      781 agacgaacca taccccaata gctattttga tttccccgtt aaataaactt ttaaccgttg
      841 cacggacaaa ctatataagc taactctttc ggcaggaaac ccgacgtatg taactggtt
//
LOCUS       DACEUZ010000330          880 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.guided.259, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000330 DACEUZ010000000
VERSION     DACEUZ010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..880
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.guided.259"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            106..591
                     /gene="vanZ"
                     /locus_tag="IXG44_002731"
     CDS             106..591
                     /gene="vanZ"
                     /locus_tag="IXG44_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HAQ1659403.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
BASE COUNT          298 a          124 c          151 g          307 t
ORIGIN      
        1 tagcactttt ggggtaacgt tagcttttta atttaaaacc cacgttaact aggacattgc
       61 tatactaatg atacaactta aacaaaagaa ttagaggaaa ttatattggg aaaaatatta
      121 tctagaggat tgctagcttt atatttagtg acactaatct ggttagtgtt attcaaatta
      181 caatacaata ttttatcagt atttaattat catcaaagaa gtcttaactt gactccattt
      241 actgctactg ggaatttcag agagatgata gataatgtta taatctttat tccatttggc
      301 ttgcttttga atgtcaattt taaagaaatc ggatttttac ctaagtttgc ttttgtactg
      361 gttttaagtc ttacttttga aataattcaa tttatcttcg ctattggagc gacagacata
      421 acagatgtaa ttacaaatac tgttggaggc tttcttggac tgaaattata tggtttaagc
      481 aataagcata tgaatcaaaa aaaattagac agagttatta tttttgtagg tatacttttg
      541 ctcgtattat tgctcgttta ccgtacccat ttaagaataa attacgtgta agatgtctaa
      601 atcaagcaat ctgatctttc atacacataa agatattgaa tgaattggat tagatggaaa
      661 acgggatgtg gggaaactcg cccgtaggtg tgaagtgagg ggaaaaccgg tgataaagta
      721 aaaagcttac ctaacactat agtaacaaag aaagcccaat tatcaatttt agtgctgagg
      781 aattggtctc tttaataaat ttccttaacg ttgtaaatcc gcattttcct gacggtaccc
      841 ccaaactaag atttcataga tagattttct gataacttta
//
LOCUS       DACEUZ010000331          833 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.guided.263, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000331 DACEUZ010000000
VERSION     DACEUZ010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..833
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.guided.263"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            10..804
                     /locus_tag="IXG44_002732"
                     /note="aph(3')-IIIa"
     CDS             10..804
                     /locus_tag="IXG44_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAQ1659404.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
BASE COUNT          262 a          145 c          219 g          207 t
ORIGIN      
        1 aaataataaa tggctaaaat gagaatatca ccggaattga aaaaactgat cgaaaaatac
       61 cgctgcgtaa aagatacgga aggaatgtct cctgctaagg tatataagct ggtgggagaa
      121 aatgaaaacc tatatttaaa aatgacggac agccggtata aagggaccac ctatgatgtg
      181 gaacgggaaa aggacatgat gctatggctg gaaggaaagc tgcctgttcc aaaggtcctg
      241 cactttgaac ggcatgatgg ctggagcaat ctgctcatga gtgaggccga tggcgtcctt
      301 tgctcggaag agtatgaaga tgaacaaagc cctgaaaaga ttatcgagct gtatgcggag
      361 tgcatcaggc tctttcactc catcgacata tcggattgtc cctatacgaa tagcttagac
      421 agccgattag ccgaattgga ttacttactg aataacgatc tggccgatgt ggattgcgaa
      481 aactgggaag aagacactcc atttaaagat ccgcgcgagc tgtatgattt tttaaagacg
      541 gaaaagcccg aagaggaact tgtcttttcc cacggcgacc tgggagacag caacatcttt
      601 gtgaaagatg gcaaagtaag tggctttatt gatcttggga gaagcggcag ggcggacaag
      661 tggtatgaca ttgccttctg cgtccggtcg atcagggagg atatcgggga agaacagtat
      721 gtcgagctat tttttgactt actggggatc aagcctgatt gggagaaaat aaaatattat
      781 attttactgg atgaattgtt ttagtaccta gatttagatg tctaaaaagc ttt
//
LOCUS       DACEUZ010000332          815 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.guided.264, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000332 DACEUZ010000000
VERSION     DACEUZ010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..815
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.guided.264"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            34..561
                     /locus_tag="IXG44_002733"
     CDS             34..561
                     /locus_tag="IXG44_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ1659405.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(624..>815)
                     /locus_tag="IXG44_002734"
     CDS             complement(624..>815)
                     /locus_tag="IXG44_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAQ1659406.1"
                     /translation="VAAVPLYHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVW
                     HLFVMLGAGFMYFSVLFFT"
BASE COUNT          280 a          149 c          177 g          209 t
ORIGIN      
        1 ctatcgctgt ttagaatcag aaaggatgag aaaatgttag ctgcaatttg ggcacaggat
       61 gaaaacggat tgattggaaa agaagaccgt cttccttggc gtttgccaaa tgatctgaaa
      121 ttcttcaaac aaatgacaga agcaaataca ctagtgatgg gacgaaaaac attcgaaggg
      181 atgggtagcc gtccgttgcc gaatcgtcag acgattgttt tgacgagaga ttcttcctat
      241 aaagcagaag gcgtgcatgt catgcatagt gtggaagacg tgcttgctct ggaaaaagaa
      301 acagacggta tctttttcat cgctggaggg tcagctgttt atcaagaatt tctgccattt
      361 tgtacgatat tataccgtac agtcatccat catgtattcg atggagatgc ttattttcca
      421 cctgttgact ggtctgattg gtcactgatc aatactagtc aaggagaaat ggatgaaaaa
      481 aatcgttatc cgcatcaatt tgaaacgtat cagcgtaatg agaatcctgt aaaaagcggg
      541 acaggcgaat tgacgatata aaaaagctat ggatagacta gctgttattt agagctgtcc
      601 catccatagc tttatttttt ttattaagta aagaataaaa cagagaagta cataaaacct
      661 gcacctaaca taacaaacaa atgccaaaca acatgcatat agcggataga attcaagcta
      721 taaaagaatg cgcctatcgt gtaagaaagc ccgcctgcta ccatcaaaat aacaccagtc
      781 attccaagag agtgataaag aggaacagca gccac
//
LOCUS       DACEUZ010000333          704 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain HOU503 isolate Bioreactor Run
            2 Day 5 SAMN10983750-rid9591253.guided.268, whole genome shotgun
            sequence.
ACCESSION   DACEUZ010000333 DACEUZ010000000
VERSION     DACEUZ010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10983750
            Sequence Read Archive: SRR8602228
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705442.1
            Genome Coverage       :: 237x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:32:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,659
            CDSs (with protein)               :: 2,659
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 24 of 47
            Pseudo Genes (incomplete)         :: 14 of 47
            Pseudo Genes (internal stop)      :: 17 of 47
            Pseudo Genes (multiple problems)  :: 7 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..704
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10983750-rid9591253.guided.268"
                     /strain="HOU503"
                     /isolate="Bioreactor Run 2 Day 5"
                     /isolation_source="infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Houston"
                     /lat_lon="29.7175 N 95.4018 W"
                     /collection_date="2017"
                     /collected_by="Amy Prater"
     gene            91..639
                     /gene="aac(6')"
                     /locus_tag="IXG44_002735"
     CDS             91..639
                     /gene="aac(6')"
                     /locus_tag="IXG44_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAQ1659407.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          253 a          110 c          160 g          181 t
ORIGIN      
        1 atactgattg gtagctaaag atttttgagc gagaacattt cgttttttca aaaggactgt
       61 tagaataagc aagaacaagg gggaagacgt atgataatca gtgaatttga ccgtaataat
      121 ccagtattga aagatcagct ttctgattta ctgagactga cttggccgga agaatatgga
      181 gacagctcgg cagaagaagt agaagaaatg atgaatccag aacgaatcgc ggtagcagcg
      241 gtagaccaag atgagttagt aggatttatt ggtgcaatcc ctcaatacgg tatcacaggt
      301 tgggaattgc atccattagt tgtagaaagc tcccgacgaa agaaccaaat aggtactcga
      361 ttagtcaatt acttagaaaa agaagtagct tccagaggag gaatcacgat ttatttaggt
      421 acggatgatt tagaccatgg aacaacgtta agtcaaacgg acctgtatga gcatacattt
      481 gataaagtgg cttccatcca gaaccttcgt gaacatccgt atgaattcta tgaaaaatta
      541 ggttataaaa tcgtaggtgt cttaccaaat gcaaatggct gggacaaacc ggatatttgg
      601 atggcaaaaa cgattattcc tcgaccaaat tctcaataat aaaaagaggt tgtgacagaa
      661 gtaatcagct tcaagaaata agaaggaatt tacgaaaatt actt
//
